LOCUS       DAWHRA010000001        63819 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWHRA010000001 DAWHRA010000000
VERSION     DAWHRA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 63819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.001"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..904
                     /gene="dprA"
                     /locus_tag="WIB30_000001"
     CDS             <1..904
                     /gene="dprA"
                     /locus_tag="WIB30_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ3796157.1"
                     /translation="LAQGLTAPPVLFLRGNVRLLHKPSAAIVGSRYATPQAMRIAKDF
                     GRALGGKGIPVVSGMASGIDTAAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIA
                     EKGLIVSEFPIGTRPYAGNFPRRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGRE
                     VMAVPGSIDNPHSKGCHKLIKDGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDT
                     GRRAVQTAYAPPPAAKMPSEAAAGGTAHGGILDKMGFDPVHPDVLAEQLAMPAADLYA
                     ALLELELDGSVAAMPGGRYQRIRT"
     gene            930..1391
                     /locus_tag="WIB30_000002"
     CDS             930..1391
                     /locus_tag="WIB30_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ3796158.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            1463..3769
                     /gene="topA"
                     /locus_tag="WIB30_000003"
     CDS             1463..3769
                     /gene="topA"
                     /locus_tag="WIB30_000003"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ3796159.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(3842..4537)
                     /locus_tag="WIB30_000004"
     CDS             complement(3842..4537)
                     /locus_tag="WIB30_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796160.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(4557..5108)
                     /locus_tag="WIB30_000005"
     CDS             complement(4557..5108)
                     /locus_tag="WIB30_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796161.1"
                     /translation="MEFLSLYPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            5226..5603
                     /locus_tag="WIB30_000006"
     CDS             5226..5603
                     /locus_tag="WIB30_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3796162.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            5692..6261
                     /gene="rsmD"
                     /locus_tag="WIB30_000007"
     CDS             5692..6261
                     /gene="rsmD"
                     /locus_tag="WIB30_000007"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ3796163.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGMEAASRHAKRIVIADNNRQTVQTLEKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            6384..6635
                     /locus_tag="WIB30_000008"
     CDS             6384..6635
                     /locus_tag="WIB30_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ3796164.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            6733..6816
                     /locus_tag="WIB30_000009"
     tRNA            6733..6816
                     /locus_tag="WIB30_000009"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6767..6769,aa:Tyr,seq:gta)
     gene            6847..6920
                     /locus_tag="WIB30_000010"
     tRNA            6847..6920
                     /locus_tag="WIB30_000010"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6879..6881,aa:Gly,seq:tcc)
     gene            6930..7004
                     /locus_tag="WIB30_000011"
     tRNA            6930..7004
                     /locus_tag="WIB30_000011"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6962..6964,aa:Thr,seq:ggt)
     gene            7053..8237
                     /gene="tuf"
                     /locus_tag="WIB30_000012"
     CDS             7053..8237
                     /gene="tuf"
                     /locus_tag="WIB30_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ3796165.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            8244..8319
                     /locus_tag="WIB30_000013"
     tRNA            8244..8319
                     /locus_tag="WIB30_000013"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8277..8279,aa:Trp,seq:cca)
     gene            8434..8712
                     /gene="secE"
                     /locus_tag="WIB30_000014"
     CDS             8434..8712
                     /gene="secE"
                     /locus_tag="WIB30_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ3796166.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            8714..9250
                     /gene="nusG"
                     /locus_tag="WIB30_000015"
     CDS             8714..9250
                     /gene="nusG"
                     /locus_tag="WIB30_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ3796167.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            9351..9785
                     /gene="rplK"
                     /locus_tag="WIB30_000016"
     CDS             9351..9785
                     /gene="rplK"
                     /locus_tag="WIB30_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ3796168.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            9785..10480
                     /gene="rplA"
                     /locus_tag="WIB30_000017"
     CDS             9785..10480
                     /gene="rplA"
                     /locus_tag="WIB30_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ3796169.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            10710..11210
                     /gene="rplJ"
                     /locus_tag="WIB30_000018"
     CDS             10710..11210
                     /gene="rplJ"
                     /locus_tag="WIB30_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ3796170.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            11268..11639
                     /gene="rplL"
                     /locus_tag="WIB30_000019"
     CDS             11268..11639
                     /gene="rplL"
                     /locus_tag="WIB30_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ3796171.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            11829..16007
                     /gene="rpoB"
                     /locus_tag="WIB30_000020"
     CDS             11829..16007
                     /gene="rpoB"
                     /locus_tag="WIB30_000020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ3796172.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAAEKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            16164..20339
                     /gene="rpoC"
                     /locus_tag="WIB30_000021"
     CDS             16164..20339
                     /gene="rpoC"
                     /locus_tag="WIB30_000021"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ3796173.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            20631..20888
                     /locus_tag="WIB30_000022"
     CDS             20631..20888
                     /locus_tag="WIB30_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796174.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMGMPINIG"
     gene            20885..21034
                     /locus_tag="WIB30_000023"
     CDS             20885..21034
                     /locus_tag="WIB30_000023"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ3796175.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            21213..21584
                     /gene="rpsL"
                     /locus_tag="WIB30_000024"
     CDS             21213..21584
                     /gene="rpsL"
                     /locus_tag="WIB30_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ3796176.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            21702..22172
                     /gene="rpsG"
                     /locus_tag="WIB30_000025"
     CDS             21702..22172
                     /gene="rpsG"
                     /locus_tag="WIB30_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ3796177.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            22191..24296
                     /gene="fusA"
                     /locus_tag="WIB30_000026"
     CDS             22191..24296
                     /gene="fusA"
                     /locus_tag="WIB30_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ3796178.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEVDKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            24382..25566
                     /gene="tuf"
                     /locus_tag="WIB30_000027"
     CDS             24382..25566
                     /gene="tuf"
                     /locus_tag="WIB30_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ3796179.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILSKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            25584..25895
                     /gene="rpsJ"
                     /locus_tag="WIB30_000028"
     CDS             25584..25895
                     /gene="rpsJ"
                     /locus_tag="WIB30_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ3796180.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            26037..26309
                     /locus_tag="WIB30_000029"
                     /pseudo
     CDS             26037..26309
                     /locus_tag="WIB30_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            26573..27217
                     /gene="rplC"
                     /locus_tag="WIB30_000030"
     CDS             26573..27217
                     /gene="rplC"
                     /locus_tag="WIB30_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ3796181.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            27217..27837
                     /gene="rplD"
                     /locus_tag="WIB30_000031"
     CDS             27217..27837
                     /gene="rplD"
                     /locus_tag="WIB30_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ3796182.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            27834..28148
                     /gene="rplW"
                     /locus_tag="WIB30_000032"
     CDS             27834..28148
                     /gene="rplW"
                     /locus_tag="WIB30_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ3796183.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANAAKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            28154..28987
                     /gene="rplB"
                     /locus_tag="WIB30_000033"
     CDS             28154..28987
                     /gene="rplB"
                     /locus_tag="WIB30_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ3796184.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            28994..29272
                     /gene="rpsS"
                     /locus_tag="WIB30_000034"
     CDS             28994..29272
                     /gene="rpsS"
                     /locus_tag="WIB30_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ3796185.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            29281..29610
                     /gene="rplV"
                     /locus_tag="WIB30_000035"
     CDS             29281..29610
                     /gene="rplV"
                     /locus_tag="WIB30_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ3796186.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            29620..30312
                     /gene="rpsC"
                     /locus_tag="WIB30_000036"
     CDS             29620..30312
                     /gene="rpsC"
                     /locus_tag="WIB30_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ3796187.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            30296..30712
                     /gene="rplP"
                     /locus_tag="WIB30_000037"
     CDS             30296..30712
                     /gene="rplP"
                     /locus_tag="WIB30_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ3796188.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            30712..30903
                     /gene="rpmC"
                     /locus_tag="WIB30_000038"
     CDS             30712..30903
                     /gene="rpmC"
                     /locus_tag="WIB30_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ3796189.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            30903..31166
                     /gene="rpsQ"
                     /locus_tag="WIB30_000039"
     CDS             30903..31166
                     /gene="rpsQ"
                     /locus_tag="WIB30_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ3796190.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            31388..31756
                     /gene="rplN"
                     /locus_tag="WIB30_000040"
     CDS             31388..31756
                     /gene="rplN"
                     /locus_tag="WIB30_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ3796191.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            31768..32091
                     /gene="rplX"
                     /locus_tag="WIB30_000041"
     CDS             31768..32091
                     /gene="rplX"
                     /locus_tag="WIB30_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ3796192.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            32101..32640
                     /gene="rplE"
                     /locus_tag="WIB30_000042"
     CDS             32101..32640
                     /gene="rplE"
                     /locus_tag="WIB30_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ3796193.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            32643..32948
                     /gene="rpsN"
                     /locus_tag="WIB30_000043"
     CDS             32643..32948
                     /gene="rpsN"
                     /locus_tag="WIB30_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ3796194.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            32964..33356
                     /gene="rpsH"
                     /locus_tag="WIB30_000044"
     CDS             32964..33356
                     /gene="rpsH"
                     /locus_tag="WIB30_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ3796195.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            33370..33903
                     /gene="rplF"
                     /locus_tag="WIB30_000045"
     CDS             33370..33903
                     /gene="rplF"
                     /locus_tag="WIB30_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ3796196.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGAEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            33917..34270
                     /gene="rplR"
                     /locus_tag="WIB30_000046"
     CDS             33917..34270
                     /gene="rplR"
                     /locus_tag="WIB30_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ3796197.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            34288..34806
                     /gene="rpsE"
                     /locus_tag="WIB30_000047"
     CDS             34288..34806
                     /gene="rpsE"
                     /locus_tag="WIB30_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ3796198.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            34799..34984
                     /gene="rpmD"
                     /locus_tag="WIB30_000048"
     CDS             34799..34984
                     /gene="rpmD"
                     /locus_tag="WIB30_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ3796199.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            34986..35420
                     /gene="rplO"
                     /locus_tag="WIB30_000049"
     CDS             34986..35420
                     /gene="rplO"
                     /locus_tag="WIB30_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ3796200.1"
                     /translation="MFLNTIQPAEGATHASRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIAFGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            35432..36742
                     /gene="secY"
                     /locus_tag="WIB30_000050"
     CDS             35432..36742
                     /gene="secY"
                     /locus_tag="WIB30_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ3796201.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLVVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            36747..36965
                     /gene="infA"
                     /locus_tag="WIB30_000051"
     CDS             36747..36965
                     /gene="infA"
                     /locus_tag="WIB30_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ3796202.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            36986..37099
                     /gene="rpmJ"
                     /locus_tag="WIB30_000052"
     CDS             36986..37099
                     /gene="rpmJ"
                     /locus_tag="WIB30_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ3796203.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            37165..37527
                     /gene="rpsM"
                     /locus_tag="WIB30_000053"
     CDS             37165..37527
                     /gene="rpsM"
                     /locus_tag="WIB30_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ3796204.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            37547..37942
                     /gene="rpsK"
                     /locus_tag="WIB30_000054"
     CDS             37547..37942
                     /gene="rpsK"
                     /locus_tag="WIB30_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ3796205.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            37962..38582
                     /gene="rpsD"
                     /locus_tag="WIB30_000055"
     CDS             37962..38582
                     /gene="rpsD"
                     /locus_tag="WIB30_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ3796206.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            38608..39594
                     /gene="rpoA"
                     /locus_tag="WIB30_000056"
     CDS             38608..39594
                     /gene="rpoA"
                     /locus_tag="WIB30_000056"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ3796207.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            39618..39986
                     /gene="rplQ"
                     /locus_tag="WIB30_000057"
     CDS             39618..39986
                     /gene="rplQ"
                     /locus_tag="WIB30_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ3796208.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            40133..40846
                     /gene="minC"
                     /locus_tag="WIB30_000058"
     CDS             40133..40846
                     /gene="minC"
                     /locus_tag="WIB30_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ3796209.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            40874..41689
                     /gene="minD"
                     /locus_tag="WIB30_000059"
     CDS             40874..41689
                     /gene="minD"
                     /locus_tag="WIB30_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ3796210.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            41693..41956
                     /gene="minE"
                     /locus_tag="WIB30_000060"
     CDS             41693..41956
                     /gene="minE"
                     /locus_tag="WIB30_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ3796211.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            41960..42880
                     /gene="oxyR"
                     /locus_tag="WIB30_000061"
     CDS             41960..42880
                     /gene="oxyR"
                     /locus_tag="WIB30_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ3796212.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(42877..43068)
                     /locus_tag="WIB30_000062"
     CDS             complement(42877..43068)
                     /locus_tag="WIB30_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796213.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            43454..43843
                     /locus_tag="WIB30_000063"
     CDS             43454..43843
                     /locus_tag="WIB30_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3796214.1"
                     /translation="MRYTIKTFQTLADETFPSKNFKILSFNGVARPITIVCPTHGVQT
                     VSAGQAFIRSKHGCPACGLVLSTSVLASRGRSVSILDTATGETLSFPSVQAAAKALNT
                     SYGSIRTKLDGRSSPDNLVCNRYKVMP"
     gene            complement(44213..47050)
                     /locus_tag="WIB30_000064"
     CDS             complement(44213..47050)
                     /locus_tag="WIB30_000064"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ3796215.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     NLDREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQVEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGRVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKNADSIMLARFPEADGGDIVQTVFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(47147..47956)
                     /gene="zupT"
                     /locus_tag="WIB30_000065"
     CDS             complement(47147..47956)
                     /gene="zupT"
                     /locus_tag="WIB30_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ3796216.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(48137..49393)
                     /locus_tag="WIB30_000066"
     CDS             complement(48137..49393)
                     /locus_tag="WIB30_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ3796217.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(49472..50863)
                     /locus_tag="WIB30_000067"
     CDS             complement(49472..50863)
                     /locus_tag="WIB30_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ3796218.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(51220..51996)
                     /gene="lpxA"
                     /locus_tag="WIB30_000068"
     CDS             complement(51220..51996)
                     /gene="lpxA"
                     /locus_tag="WIB30_000068"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ3796219.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFTQSTRGIIR"
     gene            complement(52039..52719)
                     /locus_tag="WIB30_000069"
     CDS             complement(52039..52719)
                     /locus_tag="WIB30_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ3796220.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINKFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(52926..53375)
                     /gene="fabZ"
                     /locus_tag="WIB30_000070"
     CDS             complement(52926..53375)
                     /gene="fabZ"
                     /locus_tag="WIB30_000070"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ3796221.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVS
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(53411..54454)
                     /gene="lpxD"
                     /locus_tag="WIB30_000071"
     CDS             complement(53411..54454)
                     /gene="lpxD"
                     /locus_tag="WIB30_000071"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ3796222.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEWRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVHIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            complement(54487..54987)
                     /locus_tag="WIB30_000072"
     CDS             complement(54487..54987)
                     /locus_tag="WIB30_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ3796223.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(55053..57446)
                     /gene="bamA"
                     /locus_tag="WIB30_000073"
     CDS             complement(55053..57446)
                     /gene="bamA"
                     /locus_tag="WIB30_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ3796224.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKIGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(57503..58843)
                     /gene="rseP"
                     /locus_tag="WIB30_000074"
     CDS             complement(57503..58843)
                     /gene="rseP"
                     /locus_tag="WIB30_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ3796225.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVV
                     GGVEKGSPADKAGLQPDDRLTAADGKPIASWQEWANLTRQSPGRKIALTYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(58878..60113)
                     /locus_tag="WIB30_000075"
     CDS             complement(58878..60113)
                     /locus_tag="WIB30_000075"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ3796226.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFQPEYAVVADAEYAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NSAAVLPVDSEHNAVFQVLPRDYTGRLNEHGIASIILTASGGPFLTTDLGTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(60118..60915)
                     /locus_tag="WIB30_000076"
     CDS             complement(60118..60915)
                     /locus_tag="WIB30_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ3796227.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(60918..61664)
                     /locus_tag="WIB30_000077"
     CDS             complement(60918..61664)
                     /locus_tag="WIB30_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ3796228.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(61720..62277)
                     /gene="frr"
                     /locus_tag="WIB30_000078"
     CDS             complement(61720..62277)
                     /gene="frr"
                     /locus_tag="WIB30_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ3796229.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(62457..63584)
                     /locus_tag="WIB30_000079"
     CDS             complement(62457..63584)
                     /locus_tag="WIB30_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ3796230.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHSVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(63660..63735)
                     /locus_tag="WIB30_000080"
     tRNA            complement(63660..63735)
                     /locus_tag="WIB30_000080"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63700..63702),aa:Thr,seq:cgt)
BASE COUNT        16016 a        14711 c        16772 g        16320 t
ORIGIN      
        1 gctggcgcag gggctgaccg cgccgccagt tttgtttttg cgcggcaacg tgcggctgct
       61 gcacaagcct tccgccgcca tcgtcggcag ccgctatgcc acgccgcagg cgatgcggat
      121 tgccaaagat ttcggcaggg cgttgggtgg gaaaggcatt cccgtcgtgt cgggtatggc
      181 ttcgggcatc gacaccgccg cccatcaggg cgcgttgcag gcagaaggcg gcaccatcgc
      241 cgtgtggggg acgggcatag accgcattta tccgccgtcc aataaaaacc ttgcctatga
      301 aatcgccgaa aaagggctga ttgtcagcga gttccccatc ggtacgcggc cgtatgccgg
      361 caactttccg cgccgcaacc gcctgattgc cgcgctgtcg caggttacgc tggtggttga
      421 agctgcgttg gaatccggtt cgctgattac cgccggcttg gcggcagaaa tggggcgcga
      481 agtgatggcg gtacccggtt cgatagacaa tccgcacagc aaaggctgcc acaaactgat
      541 taaagacggc gcgaaattgg tggaatgcct ggacgacatc ctgaacgaat gcccggggct
      601 attgcaaaat acgggtgctt catcatattc tataaataag gatacgcccg acacgggacg
      661 ccgcgccgtt cagacggcat acgccccgcc gcctgccgca aaaatgccgt ctgaagcggc
      721 ggcaggcggc acagcccacg gcggcatact ggataaaatg ggtttcgacc ccgtccatcc
      781 cgacgtgctt gccgagcagc ttgctatgcc tgccgcagat ttgtatgccg cactgttgga
      841 attggaattg gacggcagcg ttgccgcaat gcccggcggc agataccagc gtatccgaac
      901 ttgaacgcac tttatattaa ggaacacgaa tgaccgaagt catcgcctac ctcatcgaac
      961 atttccaaga tttcgatacc tgtccgccgc ccgaagactt gggtatgctg cttgaagaag
     1021 cgggtttcga taccatggaa atcggcaaca ccctgatgat gatggaagta ttgctcaaca
     1081 gctccgaatt ttccgccgaa cctgccgaca gcggcgcatt gcgcgtgtac agcaaagaag
     1141 aaaccgacaa cctgccgcag gaagtgatgg ggctgatgca gtatctgatt gaagaaaaag
     1201 ccgtcagctg cgaacagcgg gaaatcatca tccacgcgct gatgcacatc cccggcgacg
     1261 aaattaccgt agataccgcc aaagtgctga ccctgctgct tttatgggca aacaagagcg
     1321 agctgcccgt gttggtcggc gacgagctga tgagcgcgct tttactcgac aacaaaccca
     1381 cgatgaactg aagcggcttc agacggcccg cccgaggccg tctgaaacgc cggcatcaaa
     1441 accaccatcc agagaacgac aaatggcgaa aaacctatta atcgtcgaat ccccgtccaa
     1501 agccaaaacc ctgaaaaaat atttgggcgg cgattttgaa atccttgcat cctacggaca
     1561 cgtccgcgac ctcgtcccca aaagcggcgc ggtcgatccc gacaacggct ttgcgatgaa
     1621 ataccaactc atcagccgca acggcaaaca cgtcgatgcc atcgtcgccg gtgccaaaga
     1681 agctgaaaac atctacctcg ccaccgaccc ggatagggaa ggcgaagcca tttcctggca
     1741 tcttttggaa atcctcaaat ccaaacgcgg cttgaaaaac atcaagccgc agcgtgtcgt
     1801 gttccacgaa atcaccaaaa acgccgtgct cgatgccgtt gcccatccgc gcgaaatcga
     1861 aatggacttg gtcgatgcgc aacaagcccg ccgtgctttg gactatttgg tcggctttaa
     1921 cctttcgcca ttgttatgga aaaaaatccg ccgcggtttg agcgcgggtc gtgtgcaaag
     1981 tcccgctttg cgcctgattt gcgaacgcga aaacgaaatc cgcgcgtttg aagcgcagga
     2041 atattggacg gtgcatctcg acagccacaa aggccgcagc aagttcaccg ccaaactcgc
     2101 ccaatacaac ggcgcgaaac tcgaacaatt cgacctgccg aacgaagccg cgcaagccga
     2161 cgtattgaaa gaactcgaag gcaaagaagc cgtcgttacc gccattgaaa agaaaaagcg
     2221 cagccgcaat cctgccgcgc cgttcaccac ttctaccatg cagcaggatg ccgtgcgcaa
     2281 actcggcttc accaccgacc gcaccatgcg taccgcccaa cagctttatg aaggtataga
     2341 cgtagggcag ggcgcgatcg gtctgattac ctatatgcgt accgacagcg tgaatttggc
     2401 ggatgaagcg ttaaccgaaa tccgccatta cattgaaaac aaaatcggca aagaatatct
     2461 gccgagtgcc gccaaacaat acaaaaccaa atccaaaaac gcccaagaag cccacgaagc
     2521 catccgtccg acttccgtgt accgcacgcc cgaaagcgtc aaacccttcc tgagcgcaga
     2581 ccagttcaaa ctctatcaaa tgatttggca gcgcaccgtc gcctgtcaga tgacgcctgc
     2641 caaattcgac caaaccaccg tcgatattac cgtcggcaaa ggcgtattcc gcgtaaccgg
     2701 acaagtgcaa accttcgcag gctttttgag cgtgtatgaa gaaagcagcg atgacgaaga
     2761 aggcgaagac agcaaaaaac tgcccgaaat gagcgaaggc gataaattgc ccgtggacaa
     2821 actctacggc gaacaacact ttaccactcc gccgccgcgc tacaacgaag ccacgctggt
     2881 taaagccctc gaagaatacg gcatcggccg cccctcgacc tacgccagca tcatctccac
     2941 gctcaaagac cgcgaatacg ttacccttga gcaaaaacgc ttcatgccca ccgacacagg
     3001 cgacatcgtc aacaaattcc tgaccgaaca cttcgcccaa tacgtcgatt accacttcac
     3061 cgccaaactc gaagaccagc ttgacgaaat tgccgacggc aaacgccaat ggattccctt
     3121 gatggacaaa ttctggaaac cgttcatcaa acaagtggaa gaaaaagaag gaatcgaacg
     3181 cgccaaattt accacgcagg aacttgatga aacctgcccg aaatgcggcg aacacaaact
     3241 gcaaatcaaa ttcggcaaaa tgggtcgttt tgttgcgtgt gccggttatc ccgagtgcag
     3301 ctacacgcgc aatgtcaacg aaaccgccga agaggccgcc gagcgcatcg ccaaagccga
     3361 agccgaacag gccgaactcg acggacgcga gtgcccgaaa tgtggcggtc gcctagtgta
     3421 caaatacagc cgcaccggca gcaaattcat cggctgcgcc aactatccga aatgcaaaca
     3481 cgtcgagccg ctggaaaaac cgaaagatac cggcgtccag tgtccgcaat gcaaaaaagg
     3541 caacctcgtc gagcgcaaat cccgctacgg caaactgttt tacagttgca gcacctatcc
     3601 cgactgcaac tacgccactt ggaacccgcc cgttgccgaa gaatgcccga actgccattg
     3661 gccggtcttg accatcaaaa ccaccaaacg ctggggcgtc gaaaaagtct gcccgcaaaa
     3721 agaatgcggc tggaaagaac agattgaacc gcccgcgccg aaggagtaag attaggttgg
     3781 tttgaaagag aaaaggtcgt ctgaaaaatt ttcagacgac ctttgctttt ctgtgattgg
     3841 tttatttgaa tccgcgtgtt gttttaaagt ccgataaaat ccggttcatt tcaggcgcaa
     3901 acaaggcgat gtaatcgtaa gatagaccgc gactggcact gggatgggga aagcagacga
     3961 cttcgcaatc ttcaaacgat tggaatttga cattgaaacg tgtaccgtca aattcttttt
     4021 gcaccgtctc cagcggtttg gtctgcttac cgaccaactg ctcgaagcgt ggcagtacat
     4081 tttggttgtt cagaaaatcc gccaacctgc tgcccatgaa gaggatgact ttcggacgca
     4141 gtttttcgat gtggtagaga aaattatcga tgtgctcggg ttgtgtgaac ttgtcgggat
     4201 tgtcgatagt gttgccctgt gtagcagccc agttggtttg aaccagggat ttttcaaatg
     4261 caccgcccaa tccattttcg tctaaggggt gtccccacat ttcaaaccaa ttttttatcg
     4321 tattgtcgta acgccacttc tttgcctgct ctccgaaata gagggatttg tttgcaaatg
     4381 tatggtcgat tttgttttca gggagtttgt attcacctgc tacataagca gcctcatcgg
     4441 ctttactcca accccattca tacccacaaa tcattaaacc atgtttgtcg ttgtagcctt
     4501 tgaacagact gttgctcaaa ttcaaatcct tcatcatgaa ctcttccttt taaaatttaa
     4561 gagcgattga cttcaatgtt tttagatggg gtggaaaaat ccttgtgtag gcaacataaa
     4621 ttcaataaat ttcttgataa ttcgaaacct actaatagcg cacctataaa agctttttca
     4681 ttacgttcag catgacggtc acgtcgttca tattttttac gcttgctgtt ccctgttatt
     4741 acagctaagc caagtgatat ggcgagaatt gcccaaacaa tagtacttaa taacaatttt
     4801 ccccatacta tcaataagga aagaaaaaac cttttagtat tagatcgata ggttataatc
     4861 catgcccatg aaaatgcaat aagagttata cataagatac aagctgacag gatttcttta
     4921 tttttaatta aataacttag agcgatgagg atgacaggtg tcaagaaaat aatagttaca
     4981 tcccgatagc tataaaagaa aactgcccta ttttgaatgt ggagatgtgc acagaatcca
     5041 atatagctaa ggataatagt taatataata aaaaaagacc accaagggta aagagatagg
     5101 aattccatgt tttccttttt ttgtaaaaag gaaaaaattt tatctaagag ttaattgcct
     5161 aaccagccag aagaagttta aaatctatcc caataattca accatctata cagaaagttc
     5221 agcttatgga aacccacgaa aaaatccgcc tgatgcgcga attgaataaa tggtcccagg
     5281 aggatatggc ggaaaagctg gcgatgtcgg caggcgggta tgccaaaatc gaacggggcg
     5341 aaacgcagtt aaatatcccg cgtttggagc agttggctca gattttcaaa atcgatatgt
     5401 gggacttgct caaatcgggc ggtggtggga tggtgtttca gattaatgaa ggtgatagtg
     5461 gtggcgatat tgcgttgtat gcgtcgggtg atgtttcgat gaaaatagaa tttttaaaaa
     5521 tggagttgaa acactgcaaa gaaatgttgg aacaaaaaga caaagaaatc gagctgctcc
     5581 gcaagctgac cgaaaccgtt taaacagata tgccgtctga aaaaagtttt cagacggcat
     5641 attctttgac aggtcttgta taataccgtt tgaacttaca ggtttttgat tatggcggca
     5701 ggcaaacata ccaaacacag caaccgggta cgcattatcg gcgggcaatg ccggggcagg
     5761 aaattgagtt tcacatccgc cgacggactg cgtccgacac ccgacagcgt gcgtgaaaag
     5821 ctgtttaact ggctgggaca ggatttgacg ggtaaaacgg ttttggatct cttcggaggc
     5881 agcggcgcac tcggtatgga ggcggcatcg cgtcatgcaa aacggatcgt gattgcagac
     5941 aacaaccgtc aaacggtaca aacccttgag aaaaacagcc gcgaactggg tttggggcag
     6001 gtgcaaatcg tctgttcaga cggcatcgca tatttgaaga ccgtatccga acagtttgat
     6061 gttgtctttc tcgacccgcc gtttgcatgg caggactggc aaatcctgtt tgatgccttg
     6121 aagccgtgcc tgaacccccg agcattcgtc tatctcgagg cgggtacgct gccggatatt
     6181 cccgattggc tgacggaata tagagaaggg aaatcggggc agagtacatt cgaattaagg
     6241 gttttccaag tggctgaata atatgcgctt tgataatcat ttccgagttg taaacattcg
     6301 tttgcaaccg tccggttcaa aaaaaccttg tgctataatc cgcgcccgcc cggttttgat
     6361 aatttagtgg aaaaggaaaa gaaatgtcgc tttttattac cgacgagtgc atcaactgcg
     6421 acgtatgcga acccgaatgc cccaatgatg ccatttccca aggcgaggaa atttacgaaa
     6481 tcaaccccaa cctctgcacg cagtgcgtcg gacactacga tgagccgcag tgccagcagg
     6541 tttgcccggt ggactgcatc ctgattgacg aagaacatcc cgaaacccat gacgagttga
     6601 tggcgaaata cgaaaagatt atccagttta aataaattct ttttaaaaca tcaaattatg
     6661 tctgttttga aataaaatca aaaaaaaact tgacggaaaa gcaagccgct aataaactaa
     6721 cgttctcttt atggagggat tcccgagcgg tcaaaggggg cagactgtaa atctgttgcg
     6781 aaagcttcga aggttcgaat ccttctccct ccaccaaaat tcttacttgg ggcagtagcg
     6841 agtaatgcgg gtgtagctca atggtagagc agaagccttc caagcttacg gtgagggttc
     6901 gattcccttc acccgctcca aacaattagg cccatgtagc tcaggggtag agcactccct
     6961 tggtaaggga gaggccggca gttcaaatct gcccatgggc accatctctc gattattcat
     7021 ttctttaagg cttagatata taggatattg ccatggctaa ggaaaaattc gaacgtagca
     7081 aaccgcacgt aaacgttggc accatcggtc acgttgacca tggtaaaacc accctgactg
     7141 ccgctttgac tactattttg gctaaaaaat tcggtggtgc tgcaaaagct tacgaccaaa
     7201 tcgacaacgc acccgaagaa aaagcacgcg gtattaccat taacacctcg cacgtggaat
     7261 acgaaaccga aacccgccac tacgcacacg tagactgccc ggggcacgcc gactacgtta
     7321 aaaacatgat taccggcgcc gcacaaatgg acggtgcaat cctggtatgt tccgcagccg
     7381 acggccctat gccgcaaacc cgcgaacaca tcctgctggc ccgtcaagta ggcgtacctt
     7441 acatcatcgt gttcatgaac aaatgcgaca tggtcgacga tgccgagctg ctggaactgg
     7501 ttgaaatgga aatccgcgac ctgctgtcca gctacgactt ccccggcgac gactgcccga
     7561 ttgtacaagg ttctgcactg aaagccttgg aaggcgatgc cgcttatgaa gagaaaatct
     7621 tcgaactggc tgccgcattg gacagctaca tcccgactcc cgagcgtgcc gtggacaaac
     7681 cgttcctgct gcctatcgaa gacgtgttct ccatttccgg ccgaggtaca gtagtaaccg
     7741 gccgtgtaga gcgcggtatc atccacgttg gtgacgagat cgaaatcgtc ggtctgaaag
     7801 aaacccaaaa aaccacctgt accggtgttg aaatgttccg caaactgctg gacgaaggtc
     7861 aggcgggcga caacgtaggc gtattgctgc gcggtaccaa acgtgaagac gtggaacgcg
     7921 gtcaggtatt ggccaaaccg ggtactatca ctcctcacac caaattcaaa gcagaagtgt
     7981 acgtactgag caaagaagag ggcggccgcc atactccgtt cttcgccaac taccgtccgc
     8041 aattctactt ccgtaccacc gacgtaaccg gcgcggttac tttggaagaa ggtgtggaaa
     8101 tggtaatgcc gggtgagaac gtaaccatta ccgtagaact gattgcgcct atcgctatgg
     8161 aagaaggcct gcgctttgcg attcgcgaag gcggccgtac cgtgggtgcc ggcgtggttt
     8221 cttctgttat cgcttaagtt tagaggccaa tagctcaatt ggtagagtat cggtctccaa
     8281 aaccgagggt tgggggttcg agaccctctt ggcctgccaa ataaaaaatt aaccggcctt
     8341 gtgtcggtta atttttttgt atttgttatt tagtaaactc tcttgccatt tacatggatt
     8401 gagaatagac agatgctatg atggataaat aatatgacag aacatacgcc tgaaaaaaag
     8461 aacgttaaag tggatcaact ggttgttcaa gataaagaat ctgcatctaa ttccggtaag
     8521 gaagggtttt ttgcatattt ctcaaattct tggtccgaat tcaaaaaggt ggtttggcct
     8581 aagcgtgaag atgctgtcag aatgactgta tttgttatag tgtttgttgc tgtgctttct
     8641 atatttatct atgcggcaga tacagcaatt tcgtggttat tttttgatgt attgctgaga
     8701 agggaaggtt gagatgtcga aaaaatggta tgttgtacag gcgtattcgg ggtttgagaa
     8761 gaatgtccaa cgaatattgg aagagcgcat tgcccgtgag gagatgggag attatttcgg
     8821 acaaattctg gtgcctgtag agaaagttgt tgatatccgc aatggtcgta agactattag
     8881 tgaaagaaag tcatatcctg gttatgtgct agttgagatg gaaatgacag atgactcttg
     8941 gcatcttgta aaaagcaccc cccgtgtttc cggttttatt ggagggaggg ctaatagacc
     9001 tacgccgatt agtcagagag aggctgaaat tattttacag caggttcaga ccggcataga
     9061 gaagccgaaa ccaaaagttg aatttgaggt cggtcaacag gttcgtgtaa atgaagggcc
     9121 gtttgcggat tttaacgggg tggttgagga ggtcaattat gaacggaata agttacgcgt
     9181 gtctgttcag atatttggta gagaaacacc cgttgagctg gagttcagcc aggttgaaaa
     9241 gattaactga tttttatact tgaaaaaaaa gcaataagag gatagaatca aaaattaact
     9301 tggggagcgg aaatggttcc gcgtcttacc cgtttttagg agttcgttaa gtggcaaaga
     9361 aaattatcgg ctatattaaa ctgcaaattc ctgcaggtaa agccaatcca tctcctccgg
     9421 ttggtcctgc tttgggtcag cgcggtttga atattatgga attttgtaag gcatttaatg
     9481 ctgcaaccca aggtatggag cctggcttac cgattccggt tgtgattact gcatttgcag
     9541 ataaatcatt cacatttgtg atgaaaaccc cgccagcttc tatcttgttg aaaaaggctg
     9601 ccggtttgca aaaaggtagt tctaatcctc tgaccaacaa agtgggtaaa ttgacccgtg
     9661 cccagttgga agaaattgct aaaactaaag atcctgattt gactgctgct gacttggatg
     9721 cggctgtccg tactatagca ggttctgctc gctcaatggg cttggatgtg gagggtgttg
     9781 tataatggct aaagtatcta aacgcttgaa agctcttcgc tcttctgtgg aagccaataa
     9841 attatatgca attgatgaag caattgcttt ggtaaaaaaa gcagcgactg ctaaatttga
     9901 cgagtctgtt gacgtatctt tcaacttggg cgttgatccg cgtaaatctg accaagttat
     9961 ccgtggatcg gtcgttctgc ctaaaggcac cggtaagata acccgtgtgg ctgtatttac
    10021 tcaaggtgca aatgcagaag ctgctaaaga agctggtgca gatatcgtcg gtttcgaaga
    10081 tttggctgct gaaatcaaag cgggcaatct gaactttgac gttgttattg cttctcccga
    10141 tgcaatgcgt attgttggtc aattgggtac catcttgggt cctcgtggtc tgatgccaaa
    10201 ccctaaagta ggtacggtta cccctaatgt tgccgaagca gtaaaaaatg caaaagcagg
    10261 tcaagtgcag taccgtaccg acaaggcagg tattgttcat gcaacgattg gtcgtgcttc
    10321 attcgctgaa gctgatttga aagagaactt tgatgcgttg ctggatgcta tcgttaaagc
    10381 caagcctgct gccgctaaag gtcagtatct gaaaaaagtt gctgtgtcta gcaccatggg
    10441 tttgggtatt cgcgttgata catcaagcgt aaataactaa tcttaaggaa ttttcaagca
    10501 gtttggtttt ctgggctgct tgaatttggg ctacttaaaa ttaagtagat gtccaagacc
    10561 gtagggatcg taagatttaa tcgtaactgc cctacgcaga cggtagtcct gaaacacatt
    10621 gcaagattgc ttgtaagatg tctttttagg ttaccgcgct ggtgggatat cgttttggta
    10681 tcctgtttat aaacagtggg aggtagacct tgagtctcaa tattgaaacc aagaaagtgg
    10741 cggtcgagga aattagcgcg gcaattgcta atgctcaaac cctcgtagtc gctgaatatc
    10801 gcggtatcag tgtttccagt atgactgagc ttcgtgcgaa tgcacgtaaa gaaggcgttt
    10861 atttgcgtgt tctgaaaaat actttggctc gtcgtgcagt gcaaggtact tcatttgcag
    10921 aattggccga tcaaatggtt ggtccgttgg tttacgctgc ttctgaagat gctgttgctg
    10981 ctgctaaagt gttgcaccaa ttcgcgaaaa aagatgacaa aattgtcgtt aaagccggtt
    11041 cttacaatgg cgaagtaatg aatgctgctc aggttgctga gttggcttct attccgagcc
    11101 gcgaagagct gttgtccaaa ctgttgttcg ttatgcaagc tcctgtatcg ggctttgcgc
    11161 gcggtttggc tgctttggca gagaaaaaag ccggcgaaga agccgcttaa tcgattttgt
    11221 ttctgttaat caattatttt ttaatacaat atttggagta aaatagcatg gctattacta
    11281 aagaagacat tttggaagca gttggttctt tgaccgtaat ggaattgaac gacttggtta
    11341 aagcttttga agaaaaattt ggtgtttctg ctgctgctgt tgcagttgca ggtcctgctg
    11401 gtgccggtgc tgccgatgct gaagaaaaaa ccgaatttga tgtcgttttg gcttctgccg
    11461 gcgatcaaaa agtcggcgtg attaaagttg tccgtgcaat taccggtttg ggtctgaaag
    11521 aagctaaaga catcgttgac ggcgcaccta aaaccattaa agagggtgtt tctaaagctg
    11581 aagccgaaga catccaaaaa caactggaag aagcaggcgc taaagtcgaa atcaaataat
    11641 ttgatgcttc ttatgaaggc tggcagtttt ctgccagcct tattttgctt cttaaaataa
    11701 acatcaagta ttgtttacat ttatttgcat agtttttatc aagtcattgc aaataaatgt
    11761 aaatatcaga ttgatgcgta ccgttgtttc agacggccta ttattgaaaa ttacttttcg
    11821 gagtgtgtat gaactattcg tttaccgaga aaaaacgtat ccgtaagagt tttgcaaagc
    11881 gggaaaatgt tttggaagtt cctttcctgc tagcaactca gattgattct tatgcgaaat
    11941 ttttgcaact ggaaaatgct tttgacaaac gtaccgatga cggtctgcag gcggcattta
    12001 attctatttt cccgattgtg agccataacg gttatgcgcg attggagttt gtgcattaca
    12061 cattgggcga gcctttgttc gatattcccg aatgtcagtt gcgcggaatc acttatgcag
    12121 cccccttgcg cgcgcgtatc cgtttggtga ttttggataa ggaagcatct aaaccgacgg
    12181 taaaagaagt tcgtgaaaac gaagtgtata tgggcgaaat tccgttgatg accccgagcg
    12241 gttcttttgt gattaacggc acagagcgtg tgattgtctc ccagttgcac cgttcgcccg
    12301 gcgtattctt cgagcatgac aaaggtaaga cgcactcttc cggcaaattg ttattctccg
    12361 cccgcatcat tccctaccgt ggttcatggt tggattttga atttgatccg aaagatttgc
    12421 tgtatttccg tatcgaccgc cgccgtaaaa tgccggtaac gattttgttg aaggctttgg
    12481 gctacaacaa tgagcaaatc ttggatattt tctacgacaa agaaacgttc tatttgtctt
    12541 caaacggtgt tcaaaccgat ttggtcgcag gccgtctgaa aggcgaaact gccaaggtcg
    12601 atatcttgga taaagaaggc aatgtattgg ttgccaaagg taagcgcatt actgcgaaaa
    12661 atatccgtga tattaccaat gcaggcctga cccgtttgga tgtagaaccg gaaagcctgc
    12721 tgggtaaagc attggctgcc gatctgattg attcggaaac cggcgaggta ttggcttctg
    12781 ccaatgatga aattacagaa gagttgttgg ccaaatttga tatcaacggc gtaaaagaaa
    12841 ttacgaccct ttacatcaat gagttggatc agggtgctta tatctccaac accttgcgta
    12901 cggatgagac tgccggccgg caggcggcgc gtgttgcgat ttaccgtatg atgcgtccgg
    12961 gcgaaccgcc caccgaagag gcggtcgagc aattgtttaa ccgcttgttc ttcagtgaag
    13021 acagctacga tttgtcccgc gtaggccgta tgaaatttaa tacgcgcaca tacgaacaaa
    13081 aactgtccga agcccaacaa aactcttggt acggccgcct gctgaacgaa acgtttgccg
    13141 gtgctgccga aaaaggcggt tatgtcctga gcgtcgaaga tattgtcgcc tcgattgcga
    13201 ctttggtcga gttgcgtaac ggccatggtg aagttgacga tatcgatcac ttgggcaacc
    13261 gccgagtacg ttcggtaggc gagctgactg aaaaccaatt ccgcagcggt ttggcccgtg
    13321 tggaacgtgc tgtaaaagaa cgtttgaatc aggcggaatc agaaaacttg atgccgcacg
    13381 atttgattaa tgcaaaacct gtttctgccg ctattaaaga attcttcggc tccagccaat
    13441 tgagtcagtt tatggatcag accaacccct tgtctgaagt aacccataaa cgccgcgtat
    13501 ccgcattggg tccgggcggt ttgacccgcg aacgtgcagg atttgaggtg cgggacgtgc
    13561 atccgaccca ctacggccgc gtatgtccga ttgaaacgcc tgaaggtccg aacatcggtt
    13621 tgatcaactc attgtccgtt tatgcgcgca ccaatgatta cggtttcttg gaaacgcctt
    13681 accgccgcgt tatcgacggc aaagtaaccg aggaaatcga ttacttgtct gccatcgaag
    13741 aaggccgcta tgtgattgca caggcgaatg ccgatttgga ttcagatggc aatctgattg
    13801 gcgatttggt tacctgtcgt gaaaaaggcg aaaccattat ggcaacgccc gaccgcgtcc
    13861 aatatatgga cgtggcaact ggtcaagtgg tatccgttgc agcatccctg attccgttct
    13921 tggaacatga tgacgcgaac cgcgcattga tgggtgccaa catgcaacgt caggcagtgc
    13981 cttgcttgcg tcctgaaaaa ccgatggtcg gtaccggtat cgagcgttcc gttgccgttg
    14041 actctgctac tgcaatcgtt gcccgccgag gcggcgtggt cgagtatgtc gatgccaacc
    14101 gtgttgtgat tcgtgtccat gacgacgaag cgactgccgg cgaagtgggt gtcgatattt
    14161 acaacttggt taaatttacc cgttccaatc aatctactaa catcaaccaa cgtccggctg
    14221 tcaaagcagg cgatgttttg caacgcggcg atttggtggc cgatggcgcg tccaccgatt
    14281 tgggcgaatt ggctttgggt caaaatatga ccatcgcctt catgccgtgg aacggttaca
    14341 actacgaaga ctcgattctg atttccgaaa aagtggctgc ggacgaccgc tatacttcga
    14401 ttcacattga ggaattgaat gtcgttgccc gcgataccaa gctgggtgcg gaagacatta
    14461 cccgcgatat tccgaacttg tccgagcgta tgcaaaaccg tttggacgaa tccggtatcg
    14521 tttacatcgg tgcggaagta gaagccggcg atgtgttggt aggcaaggta acgcctaaag
    14581 gcgaaaccca gctgacgccg gaagaaaaac tgctgcgcgc catcttcggt gaaaaagcat
    14641 ctgacgtaaa agatacttca ttgcgtatgc ctaccggcat gagcggtacc gttatcgacg
    14701 ttcaagtctt cactcgtgaa ggtattcaac gcgacaaacg tgctcaatcc attatcgatt
    14761 ccgagttgaa acgctaccgt ttggatttga acgaccaatt gcgtattttc gacaacgacg
    14821 cattcgaccg tatcgagcgt atgattgtcg gtcagaaagc caacggcggc ccgatgaagc
    14881 tggccaaagg cagtgaaatc acgaccgaat atctggcggg tctgccgagc aggcacgatt
    14941 ggttcgatat ccgtctgacc gatgaagatt tggccaagca gttggaactg attaaagtga
    15001 gcctgcaaca aaaacgcgaa gaagcggacg agttatacga aatcaagaag aaaaaactga
    15061 cccaaggcga cgaattgcaa cccggcgtac aaaaaatggt gaaagttttt atcgccatca
    15121 aacgccgtct gcaagccggc gacaaaatgg cgggccgcca cggtaacaaa ggcgtggtat
    15181 cgcgcattct gccagtggaa gacatgcctt acatggcgga cggccgtccg gtagacatcg
    15241 tactgaaccc attgggcgta ccttcccgta tgaacatcgg tcagattttg gaagttcact
    15301 tgggttgggc agcaaaaggt atcggcgagc gtatcgaccg tatgctgaaa gagcaacgca
    15361 aagcaggcga gttgcgcgag ttcttgaaca aactctacaa cggcagcggc aagaaagaag
    15421 atttggatgc cctgactgat gaagaaatca tcgaactggc ctccaacctg cgtaaaggtg
    15481 catctttcgc ctctcctgta ttcgacggtg cgaaagagtc tgaaatccgc gaaatgttga
    15541 acttggctta tccgagcgac gatcctgagg ttgaaaaact gggcttcaac gacagtaaaa
    15601 cccaaatcac gctgtatgac ggccgttcag gcgaagcatt tgaccgcaag gttacagtcg
    15661 gtgtgatgca ctatctgaaa ctgcaccact tggttgacga aaaaatgcac gcgcgttcta
    15721 ccggtccgta cagtctggtt acccagcagc ctttgggcgg taaagcccag ttcggcggcc
    15781 aacgtttcgg cgagatggag gtttgggcat tggaagcata cggcgcggca tacacgctgc
    15841 aagagatgct gactgtgaag tctgacgacg tgaacggccg taccaaaatg tacgaaaaca
    15901 tcgtcaaagg cgaacacaaa atcgatgccg gtatgcccga gtccttcaac gtattggtca
    15961 aagagattcg ctcactgggc ttggatatcg atttggaacg ttactaaaca aaagttttca
    16021 gacggccttt cagggtcgtc tgaaaaagtg gtttcagaat aagaatgaag caatcggcat
    16081 ttaggccgtc tgaaatcaaa agtaccgttt ccccatatcg aaaatccgcc atgcggtaaa
    16141 aatacttcct tcaaggagca aaaatgaatt tgttgaactt atttaatccg ttgcaaactg
    16201 ccggcatgga agaagagttt gatgccatta aaatcggtat tgcctctccc gaaaccatcc
    16261 gctcatggtc ttatggcgaa gtcaaaaaac ctgaaaccat caactaccgt acgttcaaac
    16321 ctgagcgcga cggtttgttc tgtgccaaaa tctttggccc ggtcaaagac tacgaatgct
    16381 tgtgcggaaa atacaaacgc ttgaaattta aaggcgtaac gtgtgaaaaa tgcggcgtgg
    16441 aagtaaccct gtccaaagtg cgccgcgaac gcatgggtca tatcgaattg gctgcgcccg
    16501 tcgcacatat ttggttctta aaatccctgc cttcccgctt gggtatggtg ttagacatga
    16561 ctttgcgcga catcgagcgc gtattgtact ttgaagcatt tgtggtaacc gatcccggta
    16621 tgactccgct gcaacgccgc caattgctga ctgaagacga ttactacaac aagctggacg
    16681 aatacggcga cgacttcgat gccaaaatgg gtgcagaagg tatccgcgaa ttgttgcgta
    16741 ccctgaatgt agcgggcgaa atcgaaatcc tgcgccaaga gttggaatcg actggttccg
    16801 acaccaaaat caaaaaaatc gccaaacgct tgaaagtatt ggaagccttc catcgttccg
    16861 gtatgaaact ggaatggatg attatggatg tgctgccggt attgccgcct gatttgcgtc
    16921 cgttggttcc attggatggt ggtcgttttg ccacttccga tttgaacgat ttgtaccgcc
    16981 gcgttattaa ccgtaacaac cgtctgaaac gtctgttgga actgcacgcg cctgacatca
    17041 tcgtccgcaa cgaaaaacgt atgttgcaag aagcggtcga ctcgctgttg gataacggcc
    17101 gtcgcggtaa agccatgacc ggcgccaaca aacgtccgct gaaatcattg gctgacatga
    17161 ttaaaggtaa aggcggccgc ttccgtcaaa acctgctggg taaacgtgtg gactactctg
    17221 gtcgttccgt gattaccgta ggtccatacc tgcgcctgca ccaatgtggt ctgccgaaaa
    17281 aaatggcttt ggaactgttc aaaccgttca ttttccacaa attggaaaaa caaggtctgg
    17341 cttccaccgt taaagcagcg aaaaaattgg tagaacaaga agtgccggaa gtatgggaca
    17401 tcttggaaga agtcattcgc gaacatccga ttatgctgaa ccgtgcgcca accctgcacc
    17461 gtttgggtat tcaagcgttc gaacctatcc tgattgaagg taaagcgatt cagttgcacc
    17521 cattggtgtg tgccgcattt aacgccgact ttgacggtga ccaaatggct gtacacgtcc
    17581 cattgagctt ggaagcacaa atggaggcac gcacgctgat gctggcttca aacaacgtat
    17641 tgtctccggc caacggcgaa ccgattatcg taccttccca agacatcgta ttgggcctgt
    17701 actacatgac ccgcgaccgt atcaatgcca aaggcgaggg cagcctgttt gccgatgtga
    17761 aagaagtgca tcgcgcatac cataccaaac aggttgaact gggtacgaaa attaccgtac
    17821 gtctgcgcga atgggtgaaa aacgaagcag gtgagtttga gcctgtcgtt aaccgttacg
    17881 aaacaaccgt cggccgtgca ttgttgagcg aaatcctgcc gaaaggcctg ccgtttgaat
    17941 atgtcaacaa agcgttgaag aaaaaagaaa tttctaaact gattaacgca tcgttccgcc
    18001 tgtgcggctt gcgcgatacg gttatctttg ctgaccacct gatgtacacc ggtttcggat
    18061 ttgcggcaaa aggcggtatt tccattgccg ttgacgatat ggaaattcca aaagaaaaag
    18121 cggccttgct ggctgaagcc aatgccgagg ttaaagaaat cgaagaccaa taccgtcaag
    18181 gtttggttac caacggcgaa cgctacaaca aggtggtcga tatttggggt cgtgccggcg
    18241 ataagattgc taaagcgatg atggacaact tgtccaaaca aaaagttatc gaccgtgacg
    18301 gcaacgaagt cgatcaagag tcattcaact ccatttatat gatggcggac tccggtgccc
    18361 gtggttctgc agctcagatt aaacagttgt ccggtatgcg tggcttgatg gctaaacctg
    18421 acggctcgat tattgaaacg ccgattacct caaacttccg tgaaggtctg accgtattgc
    18481 aatactttat tgcgacccac ggtgcgcgta agggtttggc ggataccgca ttgaaaaccg
    18541 cgaactccgg ttacctgact cgtcgtctgg tagacgtaac tcaagatttg gtcgttgttg
    18601 aagacgattg cggtacttca gacggctttg tcatgaaggc agtggtacaa ggcggtgatg
    18661 tgattgaagc attgcgcgat cgtattttgg gtcgtgttac cgcgtctgac gttgtcgatc
    18721 cgtcaagtgg cgaaaccttg gttgaagccg gtacgttgct gactgaaaaa ctggtggata
    18781 tgatcgacca atccggtgtc gatgaagtca aagtccgtac gccgattact tgtaaaaccc
    18841 gtcacggcct gtgtgcacac tgttacggtc gtgacttggc acgcggcaaa ctggttaacg
    18901 ccggtgaggc agtcggtgtg attgctgcac aatccattgg cgaaccgggt acccagttga
    18961 ccatgcgtac gttccacatc ggtggtgcgg catcccgtgc ggcagcagcc agccaagtgg
    19021 aagccaaatc caacggtacg gcacgattca gcagccagat gcgctacgtt gccaacaaca
    19081 aaggcgagtt ggttgtcatc ggccgttctt gtgaagtcgt gattcacgac gatatcggcc
    19141 gtgaacgcga acgccacaaa gtaccttacg gtgccatcct gctggtacaa gacggtatgg
    19201 ccattaaagc cggtcaaacc ttggcaacct gggatccgca tacccgtccg atgattaccg
    19261 aacacgcagg tatggtgaaa ttcgaaaacg tggaagaggg cgttaccgtt gccaaacaaa
    19321 ccgatgatgt aaccggtttg tccactttgg tggtgattga cggtaaacgt cgttcctcta
    19381 gtgcttccaa actgctgcgt ccgactgtga aactcttgga cgaaaacggc gtggaaatct
    19441 gtattcccgg tacttctact ccggtatcca tggcattccc cgttggtgcg gtgattaccg
    19501 tacgcgaagg tcaggaaatc ggtaaaggcg acgtattggc gcgtattccg caagcctctt
    19561 ccaaaacccg cgacattacc ggcggcctgc cgcgcgttgc cgagctgttt gaagcacgcg
    19621 tgccgaaaga tgccggtatg ttggcggaaa ttaccggtac cgtttccttc ggcaaagaga
    19681 ccaaagggaa gcaacgtctg attatcactg acgtggacgg tgtagcatac gaaaccctga
    19741 tttctaaaga gaaacagatt ctggtacacg acggtcaagt ggtaaaccgc ggcgaaacca
    19801 ttgtggacgg cgcggtcgat ccgcacgata ttctgcgtct gcaaggtatc gaagcactgg
    19861 cacgctacat tgtccaagag gtgcaagagg tttaccgtct gcaaggtgtg aagatttctg
    19921 ataaacacat cgaagtcatc atccgtcaaa tgttgcgccg tgtgaacatt gcggatgccg
    19981 gcgaaaccgg gttcattacc ggagagcagg tcgaacgcgg cgatgtgatg gcggccaatg
    20041 aaaaagcttt ggaagaaggc aaagagccgg cacgttacga aaacgtattg ctgggtatta
    20101 ccaaagcctc cctgtccacc gacagtttca tttctgccgc atcgttccaa gaaacgaccc
    20161 gtgttctgac cgaagccgcg attatgggca aacaagacga actgcgcggt ttaaaagaga
    20221 acgtcatcgt cggccgcctg attcctgccg gtaccggttt gacttatcac cgcagccgtc
    20281 atcagcaatg gcaaggggta gagcaggaga ctgctgaaac ccaagtaacg gatgaataat
    20341 cttcggcgcg tttattcaat acaaaaccgc aagctttaag cttgcggttt ttctttgtcc
    20401 gattaaggca aaaacaagcg ttttcgtcat tttgaggcgt gtggattatt ccttaggtat
    20461 tttcgggccg gagaccaacg aggtggcggg tgtcgtcggt acgtccggag accaaaataa
    20521 ctttgccagg gatgttggtt tcggcggtca aaaaaagtag cgtcttaatg ttttccattt
    20581 aaacaaatgt cgtctgaaac ttcagacggc atttccttta aaaaataaat atgaaaccca
    20641 gaaatctctt ttttgcaggc tgcctgctga cttcggcgac gtttgccgag gatatcggcg
    20701 tacctgtcga actgattaac gtcggtaatc ggattgcgat gccgtctgaa ggggaaagcc
    20761 tcgccctcct gccgtttgcc gaggatgtac cgccggttcg cgatgcaatg ccgtctgaag
    20821 ttcctaaaag cgcggcaggc ggcgatgttc ggggtgaccg gatgggaatg ccgattaaca
    20881 tcggatgagc gcggctttat ggcataaaaa actgtcgtgg aaaggattta caccccaaat
    20941 aaatttccgt tacaacaaga tcaacagcaa tatgcccgcc ttttattcgc gcagcggcaa
    21001 ggaacggttt gtcagtatag aaaaaacgta ttgacagtat tttcttcagt cgtccgactg
    21061 attgtgaggg atgtcggtaa atatttatcg gcaaacaaga aaatcatctt tcttcttgtc
    21121 gttatgcttg actgtatact tgcaataaaa atataattcc actcttgccg acatggtgtc
    21181 ggcaagtatt taactcaaca ggacgagaaa atatgccaac tatcaaccaa ttggtacgca
    21241 aaggccgtca aaagcccgtg tacgtaaaca aagtgcccgc actggaagcc tgcccgcaaa
    21301 aacgcggcgt gtgcacccgt gtatacacga ctacccctaa aaaacctaac tctgcattgc
    21361 gtaaagtatg taaagtccgc ctgaccaacg gttttgaagt catttcatac atcggcggcg
    21421 aaggccacaa cctgcaagag cacagtgtcg tattgattcg cggcggtcgt gtaaaagact
    21481 tgccaggtgt gcgttaccac actgtacgcg gttctttgga tactgcaggc gttaaagacc
    21541 gtaaacaagc ccgttccaaa tacggtgcta agcgtcctaa ataattactg ggacttaaat
    21601 aggcacgtcg gccgcctaag ctgaacaacg gccgagtaag tgaatactca attgggtatt
    21661 catgggaata gacccgactg aatagattaa aggaaattaa aatgccaaga cgtagagaag
    21721 tccccaagcg cgacgtactg ccagatccta aattcggcag cgtcgagttg accaaattca
    21781 tgaacgtatt gatgattgac ggtaaaaaat ccgttgccga gcgtatcgtt tacggtgcgt
    21841 tggaacagat tgagaaaaaa accggcaaag tagcaatcga agtatttaac gaagccattg
    21901 caaacgccaa acctatcgtg gaagtgaaaa gccgccgtgt aggtggtgca aactaccaag
    21961 ttcctgttga agttcgtcct tcacgccgtt tggctttggc aatgcgctgg gttcgcgatg
    22021 cggcccgcaa acgtggtgag aaatccatgg acctgcgttt ggcaggcgaa ttgattgatg
    22081 cgtccgaagg ccgtggcggt gcgttgaaaa aacgtgaaga agtacaccgt atggctgaag
    22141 ccaacaaagc attctctcac ttccgtttct aattttgaaa ggctaataaa atggctcgta
    22201 agaccccgat cagcctgtac cgtaacatcg gtatttccgc ccatatcgat gcgggtaaaa
    22261 ccacgacgac agaacgtatt ttgttctata ccggtttgac ccacaagctg ggcgaagtgc
    22321 atgacggtgc ggctactacc gactacatgg aacaagagca agagcgcggt attaccatta
    22381 cctccgctgc cgttacttcc tactggtccg gtatggcgaa acaattcccc gagcaccgct
    22441 tcaacatcat cgacaccccg ggacacgttg actttaccgt agaggtagag cgttctatgc
    22501 gtgtattgga cggcgcggta atggtttact gcgcggtggg cggtgttcaa ccccaatctg
    22561 aaaccgtatg gcggcaagcc aacaaatacc aagtgccgcg cttggcgttt gtcaataaaa
    22621 tggaccgtca gggtgccaac ttcttccgtg ttgtcgagca aatgaaaacc cgtttgcgcg
    22681 ccaatcctgt acctatcgtc attccggttg gtgcggaaga caacttcagc ggtgtggttg
    22741 atttgttgaa aatgaaatcc atcatttgga atgaagtcga taaaggtaca acctttacct
    22801 atggcgatat tcctgccgaa ttggtcgaaa ctgccgaaga atggcgtcaa aatatgattg
    22861 aagccgcagc cgaagccagc gaagaactga tggacaaata cttaggcggc gacgagctga
    22921 ccgaagaaga aatcgtaggc gcgttgcgtc aacgtacttt ggcaggcgaa attcagccta
    22981 tgctgtgtgg ttctgcattt aaaaacaaag gtgttcaacg tatgttggac gcagttgtag
    23041 aattgctgcc agctcctacc gatattcctc cggttcaagg tgtcaacccg aataccgagg
    23101 aagccgacag ccgtcaagcc agcgatgaag agaaattctc tgcattggcg ttcaaaatgt
    23161 tgaacgacaa atacgtcggt cagctgacct ttatccgcgt ttactcaggc gtagtaaaat
    23221 ccggcgatac cgtattgaac tccgtaaaag gcactcgcga acgtatcggt cgtttggtac
    23281 aaatgactgc cgcagaccgt actgaaatcg aagaagtacg cgccggcgac atcgcagccg
    23341 ctattggtct gaaagacgtt actaccggtg aaaccttgtg tgcggaaagc gcgccgatta
    23401 tcttggaacg tatggaattc cccgagccgg taatccatat tgccgttgag ccgaaaacca
    23461 aagccgacca agagaaaatg ggtatcgccc tgaaccgctt ggctaaagaa gacccttctt
    23521 tccgtgtccg tacagacgaa gaatccggtc aaaccattat ttccggtatg ggtgagctgc
    23581 acttggaaat tattgttgac cgtatgaaac gcgaattcgg tgtggaagca aatatcggtg
    23641 cgcctcaagt ggcttaccgt gaaactatcc gcaaagccgt taaagccgaa tacaaacatg
    23701 caaaacaatc cggtggtaaa ggtcaatacg gtcacgttgt gattgaaatg gaacctatgg
    23761 aaccgggtgg tgaaggttac gagtttatcg atgaaattaa aggtggtgtg attcctcgcg
    23821 aatttattcc gtctgtcgat aaaggtatcc gcgatacgtt gcctaacggt atcgttgccg
    23881 gctatcctgt agttgacgta cgtatccgtc tggtattcgg ttcttaccat gatgtcgact
    23941 cttcccaatt ggcatttgaa ttggctgctt ctcaagcgtt taaagaaggt atgcgtcaag
    24001 catctcctgc cctgcttgag ccaatcatgg cagttgaagt ggaaaccccg gaagaataca
    24061 tgggcgacgt aatgggcgac ttgaaccgcc gtcgcggtgt tgtattgggt atggatgatg
    24121 acggtatcgg cggtaaaaaa gtccgtgccg aagtaccttt ggcagaaatg ttcggttact
    24181 cgaccgacct gcgttctgca acccaaggcc gcgctactta ctctatggag ttcaagaaat
    24241 attctgaagc tcctgcccac atagctgctg ctgtaactga agcccgtaaa ggctaatcag
    24301 aaaaggccgt ctgaaactga aaataaattt tcagacggcc attgttcttt aatcgatctt
    24361 tatatgtaaa ggaattagct catggctaag gaaaaatttg aacgtagcaa accgcacgta
    24421 aacgttggca ccatcggtca cgttgaccat ggtaaaacca ctctgactgc tgctttgact
    24481 actattttgt ctaaaaaatt cggtggcgct gcaaaagctt atgaccaaat cgacaacgct
    24541 cctgaagaaa aagctcgtgg tattaccatt aatacctcac acgtagaata cgaaactgaa
    24601 acccgtcact acgcacacgt agactgcccg gggcacgccg actacgttaa aaacatgatt
    24661 accggcgccg cacaaatgga cggtgcaatc ctggtatgtt ccgcagccga cggccctatg
    24721 ccgcaaaccc gcgaacacat cctgctggcc cgccaagtag gcgtacctta catcatcgtg
    24781 ttcatgaaca aatgcgacat ggtcgacgat gccgagctgt tggaactggt tgaaatggaa
    24841 atccgcgacc tgctgtccag ctacgacttc cccggcgatg actgcccgat tgtacaaggt
    24901 tccgcactga aagccttgga aggcgatgcc gcttacgaag aaaaaatctt cgaactggct
    24961 gccgcattgg acagctacat cccgactccc gagcgagccg tggacaaacc gttcctgctg
    25021 cctatcgaag acgtgttctc catttccggc cgcggtacag tagtaaccgg ccgtgtagag
    25081 cgcggtatca tccacgttgg tgacgagatt gaaatcgtcg gtctgaaaga aacccaaaaa
    25141 accacttgta ccggtgttga aatgttccgc aaactgctgg acgaaggtca ggcgggcgac
    25201 aacgtaggcg tattgctgcg cggtaccaaa cgtgaagacg tggaacgcgg tcaggtattg
    25261 gctaaaccgg gtactatcac tcctcacacc aaattcaaag cagaagtata cgtactgagc
    25321 aaagaagagg gtggtcgtca cactccgttc ttcgccaact accgtccgca attctacttc
    25381 cgtaccaccg acgtaaccgg cgcggttact ttggaagaag gtgtggaaat ggtaatgccg
    25441 ggtgaaaacg taaccatcac cgtagaactg attgcgccta tcgctatgga agaaggcctg
    25501 cgctttgcga ttcgcgaagg cggccgtacc gtgggtgccg gcgtggtttc ttctgttatc
    25561 gcttaattga aggatattga taaatggcaa accaaaaaat ccgtatccgc ctgaaagctt
    25621 atgattacgc cctgattgac cgttctgcac aagaaatcgt tgaaactgca aaacgtaccg
    25681 gtgcagttgt aaaaggcccg attcctttgc cgaccaaaat cgagcgtttc aacattttgc
    25741 gttctccgca catgaacaaa acttcccgtg agcaattgga aatccgcacc cacttgcgcc
    25801 tgatggacat cgtggattgg accgataaaa ctaccgatgc gctgatgaag ctggatttgc
    25861 cggccggtgt tgatgtagaa atcaaagtcc aataattcgg actataaaaa atccccaagc
    25921 aatcaatgct tggggatttt ttatgttatg ccgagacctt tgcaaaattc cccaaaatcc
    25981 cctaaattcc caccaagaca tttaggagca ccttcttcca gcaaaccgcc caagccatga
    26041 ttgccaaaca catcgaccgg ttcccactat tgaagttgga ccgggtaatt gattggcagc
    26101 cgatcgaaca gtacctgaat cgtcaaagaa cccgttacct tagagaccac cgcggccgtc
    26161 ccgcctatcc cctgttgtcc atgttcaaag ccgtcctgct cggacaatgg cacagcctct
    26221 ccgatcccga actcgagcac agcctcatca cccgcatcga tttcaacctg ttttgccgct
    26281 ttgacgaact gagcatcccc gattacagtc atcaaccata ttccggtttg tcggagaaag
    26341 atgcatacgc tgtgatgacc ggataccgac ccgttaaaag agtccgaccc tatgccgtct
    26401 gaaaattcaa aacgcttcag acggcatatt gaagatattt ctgatatttc tgttgatatt
    26461 tctttgactt gtcagatata atgccgagct tggtacattt gtgccaagtt taactttgtc
    26521 tgaaagacag gccaatcgta gcctgtccct ttactttaaa aggaaaataa tcatgacttt
    26581 aggtctggtt ggacgcaaag ttggtatgac ccgcgtgttc gacgaacagg gtgtttctgt
    26641 tccggtaacc gttttggata tgtctgccaa ccgcgttaca caagtaaaat ccaaagatac
    26701 tgacggctat actgccgttc aagttacctt tggtcagaaa aaagccaatc gtgtcaacaa
    26761 agccgaagcc gggcactttg caaaagcagg tgttgaagcc ggtcgcggtt tgattgagtt
    26821 tgctttgact gaagaaaaac tggctgaatt gaaagctggt gacgaaatca ccgtttctat
    26881 gtttgaagtc ggtcaactgg tcgatgtaac cggtacctct aaaggtaaag gtttctccgg
    26941 cacgattaaa cgtcataact tcggtgccca acgtacttcc cacggtaact cccgttctca
    27001 ccgtgttcca ggctctatcg gtatggcgca agacccgggt cgcgtgttcc ccggtaaacg
    27061 catggccggc caatacggca acaccaaagc aactgttcaa aaattggaag ttgtccgtgt
    27121 tgacgcagaa cgccaactgc tgttggttaa gggtgctgtt ccgggtgcgg tcaacagcga
    27181 tgttgtagtt cgtcccagcg tgaaagtagg tgcgtaatgg aattgaaagt aattgacgct
    27241 aaaggacaag tttcaggcag tctgtctgtt tctgatgctt tgttcgcccg cgaatacaat
    27301 gaagcgttgg ttcatcagct ggtaaatgcc tacttggcaa acgcccgctc cggtaaccgc
    27361 gctcaaaaaa cccgtgccga agtaaaacac tcaaccaaaa aaccatggcg tcaaaaaggt
    27421 accggccgtg cccgttccgg tatgacttct tctccgctgt ggcgtaaagg tggtcgcgcg
    27481 ttcccgaaca aacccgacga aaacttcact caaaaagtaa accgcaaaat gtaccgtgcc
    27541 ggtatggcga ctattctgtc ccaattgact cgtgacgagc gtttgtttgc gattgaggcg
    27601 ttgactgccg aaactcctaa aaccaaagtt tttgccgaac aagtgaaaaa tctgggtctg
    27661 gagcaagtgt tgtttgtaac caaacagctc gacgagaatg tttacttggc ttcacgcaac
    27721 ttgccaaacg tgttggtttt ggaagctcaa caagttgatc cttacagctt gctgcgttac
    27781 aaaaaagtaa tcatcactaa agatgcagtt gcacaattag aggagcaatg ggtatgaatc
    27841 aacaacgttt gactcaagtg attttggcac ctatcgtttc tgaaaaaagc aacgtattgg
    27901 ctgaaaaacg caaccaaatg acgtttaaag ttttggcaaa tgcagccaaa cctgaaatca
    27961 aagcggctgt cgagctgctg ttcggtgttc aagttgcttc tgtaactacc gttacaacta
    28021 aaggcaaaac taagcgtttt ggtcgtactt tgggccgccg cagcgatgtt aaaaaagctt
    28081 atgtaagctt ggctgccggt caagagttgg atttggaagc cgctgctgca gctgcagata
    28141 aggaataaac aaaatggcaa tcgttaaaat gaagccgacc tctgcaggcc gtcgcggcat
    28201 ggttcgcgtg gtaacagaag gtttgcacaa aggtgcacct tatgcaccct tgcttgaaaa
    28261 gaaaaattct actgccggcc gtaacaataa tggtcatatc accacccgtc acaaaggcgg
    28321 cggtcataaa caccattacc gtgttgtaga ctttaaacgt aacaaagacg gcattcctgc
    28381 caaagtagag cgtatcgaat acgatcctaa ccgtactgcc ttcatcgcac tgttgtgcta
    28441 tgcagacggc gagcgtcgtt acatcattgc tcctcgcggt attcaagccg gtgccgtatt
    28501 ggtttccggt gctgaagctg ccatcaaagt aggcaacacc ctgccgatcc gcaacattcc
    28561 cgttggtacg actatccact gtatcgaaat gaaacccggt aaaggtgctc aaatcgcacg
    28621 ttctgccggt gcttctgcgg tattgctggc taaagaaggc gcgtacgctc aagtccgtct
    28681 gcgctctggc gaagtccgta aaatcaacgt aaattgccgt gcgaccatcg gtgaagttgg
    28741 taacgaagag caaagcctga aaaaaatcgg taaagccggt gccaaccgtt ggcgcggtat
    28801 tcgtccgacc gtacgcggtg ttgtcatgaa tcctgtcgat cacccgcatg gtggtggtga
    28861 aggccgtacg ggcgaggccc gcgaaccggt cagcccatgg ggtactcctg ctaaaggcta
    28921 ccgcactcgt aataacaaac gcacggataa catgattgtt cgtcgccgtt actcaaataa
    28981 aggttaattt agtatggctc gttcattgaa aaaaggccca tatgtagacc tgcatttgct
    29041 gaaaaaagta gatgctgctc gcgcaagcaa cgacaaacgc ccgattaaaa cctggtctcg
    29101 tcgttctacc attctgcctg attttatcgg tctgaccatt gctgtgcaca acggccgcac
    29161 ccatgtgcct gtgtttatca gcgacaatat ggttggtcat aaattaggcg aattctcatt
    29221 gacccgtacc tttaaaggcc acttggccga taaaaaggct aaaaagaaat aaggtgaatc
    29281 atgagagtaa atgcacaaca taaaaatgcc cgtatctctg ctcaaaaggc tcgtttggta
    29341 gctgatttga ttcgtggtaa agacgttgcc caagctttga atattttggc tttcagtcct
    29401 aaaaaaggtg ccgagctgat taaaaaagta ttggagtcag ctattgctaa tgccgagcac
    29461 aataacggtg cggacattga tgaactgaaa gtggtaacta tctttgttga caaaggccca
    29521 agcttgaaac gttttcaagc tcgcgccaaa ggtcgcggta accgcatcga aaaacaaact
    29581 tgtcatatca atgtgacagt gggtaactaa ggaaaagcta tgggacaaaa gattaaccct
    29641 acaggctttc gcctggcggt aactaaagac tgggcttcaa aatggtttgc taaaagcacc
    29701 gacttttcta ctgttttgaa gcaggatatc gatgttcgca attatttgcg tcaaaaattg
    29761 gccaatgctt cggttggtcg agtggttatt gaacgccctg caaaatctgc acgcattacc
    29821 attcactccg ctcgtccggg tgtggttatc ggtaaaaaag gtgaggatat cgaggttttg
    29881 aaacgtgact tgcaagtctt gatgggtgta cctgttcatg taaatattga agagattcgc
    29941 cgtcctgagt tggatgctca aattattgct gacggtattg cccagcagtt ggaaaagcgc
    30001 gttcaattcc gtcgtgctat gaaacgagca atgcaaaatg caatgcgttc tggtgctaaa
    30061 ggtattaaga ttatgacttc aggccgtctg aatggtgcgg atattgcccg tagcgaatgg
    30121 tatcgtgaag gtcgcgtgcc actgcatact ttacgtgcaa atgtagatta tgcaaccagc
    30181 gaagcgcaca ccacatatgg tgtattgggt ctgaaagttt gggtttatac ggaaggcaat
    30241 attaaatctt ccaaacctga acatgagagt aaacaaagaa aggcaggtag acgtaatgct
    30301 gcagccaact agactgaaat accgtaagca acaaaagggt cgcaatactg gcatcgctac
    30361 tcgcggtaat aaggtaagtt tcggtgagtt cggcttgaaa gccgtaggtc gtggtcgttt
    30421 gactgcccgt caaatcgaag ctgctcgtcg tgcaatgacc cgtcatatca aacgtggtgg
    30481 tcgtatttgg attcgtgtat tccctgataa accgattact gaaaagccta ttcaagttcg
    30541 tatgggtggc ggtaaaggta acgtggaata ttacattgcc gaaattaaac caggtaaagt
    30601 gttgtatgaa atggatggcg ttccagagga actggctcgt gaagcattcg agttggctgc
    30661 tgccaaattg cctattccta caacctttgt agtaagacag gtgggtcaat aatgaaagca
    30721 aatgaattga aagacaaatc cgttgagcag ttgaatgcag atttgttgga cttgttgaaa
    30781 gctcagtttg gcttacgtat gcaaaacgct accggtcaat taggcaaacc aagtgaattg
    30841 aaacgtgtac gtcgcgatat tgctcgtatt aaaaccgttt taactgaaaa aggtgctaag
    30901 taatgagcga aactaaaaat gttcgtactt tgcaaggcaa agtagtaagc gacaaaatgg
    30961 ataaaaccgt aacagtattg gttgagcgta aagtaaaaca tccgctgtat ggtaagatta
    31021 ttcggttatc tactaaaatc catgcccatg atgaaaataa tcaatatgga attggtgatg
    31081 tggttgttat atcggaatcc cgtccattgt caaaaactaa atcttgggtt gtcagtgagc
    31141 tggttgagaa agcacgttct atttaagaat taaagcaacg tgcttggaat gggaaacgaa
    31201 gtattgcagc aaatttaatt tgcgtgtaaa cttcgtttcc tgtctttcag tttcttctgg
    31261 aagtttcttc cctttcgggg tccaagactg gtttacttga accgcaaggt ttcatttaat
    31321 aagcagcggc tttgctgtaa gttatctgaa agtggtaaat taagttggtt aatttaaagg
    31381 taataacatg attcaaatgc agaccatctt agatgtggct gataactctg gtgcgcgtcg
    31441 cgtaatgtgt atcaaggtat tgggcggatc taagcgtcgc tacgcttctg ttggcgatat
    31501 tattaaagtg gcagttaaag atgcggctcc gcgtggccgt gtcaaaaaag gcgatgtata
    31561 taatgcggta gttgttcgta ctgctaaggg tgtacgtcgt cctgatggtg cgttaattaa
    31621 attcgataac aatgccgccg tgttactgaa taataaactt gaacctttgg gtactcgtat
    31681 ctttggtccg gtaacccgtg aattgcgtac tgagcgattt atgaaaatcg tttcattggc
    31741 acctgaagta ttataaggaa tggcacgatg aataaaatca ttaaaggcga tagggttgta
    31801 gtaattgctg gtaaggataa aggtaagcag ggtcaagtag ttcgagtgtt gggtgataaa
    31861 gttgttgttg agggcgttaa tgttgtaaaa cgccatcaaa aacctaatcc aatgcgtggc
    31921 attgagggcg gtattattac taaagaaatg cctttggata tttctaatat cgcaatcctg
    31981 aatccggaaa ctaataaagc ggaccgtgtt ggtattaagc tgattgaaaa tgaaggcaaa
    32041 gttaaacgcg ttcgtttctt caaatcaaat ggctctatca ttggggcata aggagataac
    32101 atggctcggt tgagagagtt ttataaagag acagttgttc ctgaattggt taaacaattt
    32161 ggttacaaat cagtaatgga agtcccgcgt attgaaaaaa ttaccttgaa tatgggtgtg
    32221 ggtgaggctg ttgctgataa aaaagttatg gaacatgctg tttccgattt agagaaaatt
    32281 gccggtcaaa aaccggttgt tactgtcgcc cgtaaatcta tcgcaggttt taaaatccgt
    32341 gataactatc cggttggttg caaagtaaca ttgcgtcgtg atcaaatgtt tgaattcttg
    32401 gatcgtttga ttactattgc attacctcgc gtacgtgact tccgtggtgt gagcggtaaa
    32461 tcatttgatg gccgtggcaa ttacaatatg ggtgttcgtg agcaaattat ttttccggaa
    32521 attgaatacg ataaaattga tgctttgcgt ggtttgaata ttactattac tactacagca
    32581 aaaaccgatg aggaagcgaa agctttattg tcattgttta aatttccgtt caaaggataa
    32641 tcatggctaa gaaagcactt attaatcgtg atctgaaacg tcaagctttg gctaaaaaat
    32701 atgcggctaa acgcgcggca attaaagcgg taatcaatga ttcgaatgca actgaggaag
    32761 agcgttttga ggctcgtttg aggtttcaat ccattcctcg taatgcggca cctgtgcgtc
    32821 aacgtcgtcg ttgtgctttg acaggtcgcc ctcgtggtac tttccgtaaa tttggtttgg
    32881 gtcgtattaa aatccgtgaa atcgccatgc gtggcgaaat tccgggtgtt gttaaagcca
    32941 gctggtaata ggagtaatta agaatgagta tgcatgatcc tatttccgat atgttgactc
    33001 gtatccgcaa tgcgcaacgt gctaataaag cagcagttgc aatgccttct tcaaaattaa
    33061 agtgtgctat tgcaaaggta ttgaaagaag aaggatatat tgaggacttc gcagtttcat
    33121 ctgacgtaaa gtctatattg gaaattcaat taaaatacta tgcaggtcgt cctgtaattg
    33181 aacaaatcaa gcgtgtatct cgccctggtt tgcgtattta taaagcgtct agtgagattc
    33241 caagtgttat gaatggcttg ggtattgcta ttgttagtac ttctaaaggt gtaatgactg
    33301 atcgtaaagc acgttctcaa ggtgttggtg gtgagttgtt atgcattgta gcctagtgga
    33361 ggaaaagaaa tgtcacgtgt cgcaaaaaac ccagtgactg ttcccgctgg tgtagaagta
    33421 aaatttggag cagaggcatt agttattaag ggtaagaacg gtgaattgtc ttttcctttg
    33481 cattctgatg tagccattga atttaatgat ggcaaattga cttttgttgc gaataacagc
    33541 agtaaacaag caaatgcaat gtctggtact gctcgcgcat tagtcagcaa tatggttaaa
    33601 ggtgtttcag aaggttttga gaaaagattg caattgatag gtgtgggtta tcgtgctcaa
    33661 gcacaaggta aaatcttgaa tctgtctttg ggtttttctc atccgatcgt atatgaaatg
    33721 cctgaaggtg tctccgttca aactcctagc caaacagaga ttgttttaac cggctcggat
    33781 aaacaagttg ttggtcaagt tgctgctgag attcgtgcgt tccgtgctcc tgagccttat
    33841 aaaggtaaag gtgttcgcta tgtaggcgaa gtagtggtaa tgaaagaagc caagaaaaaa
    33901 taattgaggt tcactaatgg ataaacatac aacccgactc cgtcgtgcac gcaaaacccg
    33961 tgctcgtatt gcggacttga aaatggtaag attatgtgtg ttccgaagca ataatcatat
    34021 ttatgctcaa gtaattagtg ctgaaggtga taaagtattg gctcaagcct ctacattgga
    34081 agctgaggtg cgcggtagtc tgaaatctgg aagcaatgtt gaagcagctg caatagttgg
    34141 taaacgtatc gctgagaagg ctaaagcagc aggtgttgaa aaagttgctt ttgaccgttc
    34201 aggtttccaa tatcacggtc gtgtgaaagc tttggctgaa gctgcacgtg aaaatggttt
    34261 aagcttctaa tatttggaga ctttcagatg gcaaaacatg aaattgaaga acgcggtgac
    34321 ggcctgattg aaaagatggt cgcagttaat cgcgtaacta aagtagttaa aggtggccgt
    34381 atcatggctt tctcagcact gactgttgtt ggtgatggtg atggtcgcat tggtatgggc
    34441 aaaggtaaat caaaagaagt accagttgct gttcaaaaag caatggatca agctcgacgc
    34501 tctatgatta aagtaccttt gaaaaacggt acgattcatc atgaggttat tggccgtcat
    34561 ggtgctacta aagtatttat gcagcctgct aaagagggta gtggcgtaaa agccggtgga
    34621 cctatgcgtt tggtttttga tgctatgggc attcataata tctccgccaa agtgcacgga
    34681 tctactaacc catataatat cgtacgtgca acattagatg gtttgtctaa gttgcatact
    34741 cctgctgata tcgcagccaa acgtggcttg acagtggaag acattttggg agttaaccat
    34801 ggctgaacaa aaaaagatta gggttacatt ggttaaaagc ctgattggta caattgaatc
    34861 tcatcgtgca tgtgctcgcg gtttaggttt gcgccgccgt gaacatactg tagaggtttt
    34921 ggatacccct gaaaaccgtg gcatgatcaa taaaatcagc tacttgttga aagtggagtc
    34981 ttaatatgtt tttgaatact attcaacctg ctgagggtgc tactcacgct agtcgtcgtg
    35041 ttggacgcgg tattggtagc ggcttgggta agacaggtgg tcgtggtcat aaaggtcaaa
    35101 aaagccgttc tggtgggttt cataaggtgg gtttcgaggg tggtcaaatg cccttgcaac
    35161 gacgcctccc taaaagaggt tttaaatctt taacagcatc agctaatgca cagcttcgtt
    35221 taagtgaact ggaatcaatt gctgttaatg agattgatat tttggtctta aagcaagcgg
    35281 gtctgattgc atctacagtc tctaatgtta aagttattgc ttttggtgaa atttctaagg
    35341 cagttgcttt gaagggtatt aaagttacca aaggtgcgag agctgctatc gaggctgttg
    35401 gtggtaagat tgaaatgtaa ggtttaatat tgtggctaat caacaaacgt catcaggttc
    35461 atccaaattt ggagatctta agaaacgtct tttgtttcta tttggagcat tgattgtttt
    35521 tcgaattggt gcccatatac ccgtacctgg agttgatgct gttgctttag ctaaattata
    35581 cgaaagcgct ggaaacggca tcctgggaat attgaatatg ttttccggtg ggtcgttaga
    35641 gcgctttagt atatttgcaa taggaattat gccatatatt tcagcttcta ttattgtaca
    35701 gctcgcttct gaaattttgc catcattgaa ggctttaaaa aaagaagggg aggctggtag
    35761 aaaggtaatt acgaaatata ctaggtatgg tactgttttg ttagcaattc ttcaaagtct
    35821 aggtgttgca tctttcgtat ttcagcaagg aattgttgta acaagttcat ttgagtttca
    35881 tgtttccacg gtagtttctt tggtaacggg aaccatgttt cttatgtggc ttggggagca
    35941 aattactgaa aggggtatcg ggaacggtat ttctttaatc attacggcag gtattgcttc
    36001 aggtattcct tcgggtattg caaagctggt tacactgacg aaccaaggtt ctatgagcat
    36061 gcttacggcg ttgcttgttg tatttggtgc cttattatta atttatttag ttgtatactt
    36121 tgaaagtgca cagcggaaga ttcctattca ttatgcaaaa cgccagttta atggtagggc
    36181 gggtagtcaa aatacgcata tgcctttcaa gttgaatatg gctggtgtta ttcccccaat
    36241 ttttgcttcc agtattattc tatttccatc tactctttta ggttggtttg gttcggctga
    36301 tacaaatagt gttttgcaca aaatagctgg attgttacaa cacggtcaat tgctgtatat
    36361 ggctttattt gcagcgacag ttattttctt ttgttatttt tatacggctt tggtttttag
    36421 ccctaaagaa atggcagaga atttaaaaaa gagtggtgct tttgttcctg ggattagacc
    36481 tggtgagcag acctctaggt atttagaaaa agttgtatta cgtttgacat tgtttggagc
    36541 tctttatatt acaactattt gtttaattcc agagttctta actacggttt taaatgtacc
    36601 tttttatttg ggtggcacgt ctttgttgat tctagttgtt gtaacgatgg attttagtac
    36661 acaaataaat tcgtataggc ttactcaaca gtatgataag ttaatgactc gttcagaaat
    36721 gaaatcattt tctcggaaat agaattatgg cgaaagaaga tactatccaa atgcaaggtg
    36781 aaattcttga aactttacct aatgcaacat ttaaagtaaa acttgagaat gaccatattg
    36841 tattgggtca tatttctggg aagatgcgga tgcattacat tcgtatttct ccgggagata
    36901 aggtcacagt agagctgaca ccttatgatt taactagggc tcgaatcgtt ttcagagcaa
    36961 gataaaccaa taaaaggaaa ataaaatgcg tgtacaacca tctgttaaga aaatttgccg
    37021 aaattgcaag attattcgtc gaaatcgtgt agttcgtgta atttgtactg atctccgtca
    37081 caaacagcgt caaggttaat ggaatatttc ttttaatgtg attctgtgat atagtgacac
    37141 actttgccct aaaaaggaaa aaatatggct cgtattgcag gggtaaatat ccctaataac
    37201 gcacacatcg taattggtct tcaggctatt tacggtattg gtgctactcg tgctaaattg
    37261 atttgtgagg ctgcaaatat tgcgcctgat actaaagcaa aagatttgga cgagactcaa
    37321 ttagatgctt tgcgtgacca agttgccaag tatgaagtag aaggtgattt gcgtcgtgaa
    37381 gtaactatga gtatcaagcg attgatggac atgggctgct atcgtggctt ccgtcatcgt
    37441 cgcggcttac catgccgcgg tcaacgcact cgtacaaatg cgcgtacccg caaaggtccg
    37501 cgtaaagcga ttgctggtaa gaaataaatt ttaaggaatt ttattaatgg ctaaagcaaa
    37561 cacagcttca cgtgtacgta aaaaagtacg taaaaccgtg agtgagggta ttgtgcacgt
    37621 tcatgcatct ttcaacaata ccatcattac aatcactgac cgtcaaggca atgcgttgtc
    37681 ttgggctacc tctggcggcg ctggttttaa aggttctcgt aaaagtacac catttgcagc
    37741 acaagttgca gcagaagcag ctggtaaagt tgcccaagag tatggcgtta aaaatttaga
    37801 ggttcgtatt aaaggtccag gtccaggtcg cgaatcctct gtacgtgctt tgaatgctct
    37861 tggtttcaag attaccagca ttactgacgt taccccgttg cctcataacg gttgccgtcc
    37921 gcctaaaaaa cgtcgtattt aatattggag tgatttgaaa catggcacgt tatattggcc
    37981 ctaaatgtaa gttggcacgt cgcgaaggta cggatttgtt tttgaagagt gcgcgccgct
    38041 ctttggattc taaatgtaaa attgattccg ctcccggtca gcatggtgca aaaaaaccgc
    38101 gtttgtcaga ctatggtttg cagttgcgtg aaaaacaaaa aatccgccgt atttatggcg
    38161 tattagaacg tcagttccgt cgttatttcg cagaagctga tcgtcgtaaa ggttctaccg
    38221 gcgagttgct gttgcagttg ctggaatctc gtttggataa tgtcgtttat cgtatgggtt
    38281 tcggttctac ccgagctgaa gcaagacagc ttgtttctca taaggcgata gttgtgaatg
    38341 gacaagttgt caatattcct tctttccaag tgaaagctgg tgatgttgtc tcagttcgtg
    38401 aaaaagccaa aaaacaggta cgtattcaag aagcattggg tttggcaact caaatcggct
    38461 tgccgggttg ggtttctgta gatgcggata aacttgaggg tgtgttcaaa aacatgccgg
    38521 atcgctcgga attgaccggt gatattaatg aacagctggt ggtagagttc tactctaaat
    38581 aatgctagct cagtgaggga cagttaaatg cagaatagca caaccgaatt tttgaaacct
    38641 cgtcaaattg atgtaaatac tttttctgca actcgtgcaa aagtatctat gcagccattt
    38701 gaacgtggtt tcggtcatac cttaggtaat gctttgcgcc gtatcttact gtcatccatg
    38761 aatggttttg ctcctactga agtagctatt gccggtgtat tacacgaata ttctactgtt
    38821 gatggtattc aggaagatgt tgttgacatt ttgctgaata ttaaaggtat tgtgtttaaa
    38881 ctccatggtc gtagccaagt tcaacttgtg ttgaagaaat caggttcagg tgtcgtatct
    38941 gccggtgata ttgagttgcc gcatgatgta gaaattctga atcctggtca tgtcatttgt
    39001 catttggctg ataacggtca aattgagatg gaaattaaag tagagcaagg tcgtggttat
    39061 caatctgttt caggtcgtca ggtagttcgt gatgagaacc gtcagattgg tgcaatccag
    39121 ttggatgcga gcttttcgcc catcagccgt gttagctttg aggttgaacc tgcacgtgta
    39181 gagcagcgga cggatcttga taagttggtt ttggatatcg aaaccgacgg ttctattgat
    39241 cctgaggaag ctgtacgcag tgcggcacgt attttgattg atcagatgtc tatttttgct
    39301 gatttgcagg gtacgcctgt ggaggaggtt gaagaaaaag cacctcctat cgaccctgtt
    39361 cttttgcgtc cggtggatga tctggaattg acagtacgtt cagctaattg tttgaaagct
    39421 gaggatattt attatattgg cgatttgatt caacgcactg aaaccgagct tcttaaaacg
    39481 ccgaatttgg gacgtaaatc tttgaatgag attaaggaag tattggcatc taaaggtttg
    39541 acactgggtt ctaagttgga agcatggcca cctgtaggct tggaaaagcc ttaatgaaga
    39601 attaaaggat aattgatatg cgtcatcgta atggcaatcg caaattaaac cgtaccagca
    39661 gtcatcgtgc tgcaatgctg cgtaatatgg cgaattcatt attgactcac gaagctattg
    39721 taacaactct gcctaaggcc aaggaattgc gccgtgtagt agagccgttg attacattgg
    39781 gtaaaaagcc gtcattggca aaccgccgtt tggcatttga ccgtactcgc gaccgtgatg
    39841 ttgtagtaaa actgtttggc gatttgggtc ctcgttttac tgctcgtaac ggtggttatg
    39901 ttcgggtgtt gaaatacgga ttccgtaaag gtgataatgc acctctggca ctggttgaat
    39961 tggttgacaa accggctgct gagtaatttt agtcatataa cgccatctgc cgaaaagcag
    40021 gtggcgttat ttttgcaata tctgataggt aatagggtat tggctatcat gtttaaaata
    40081 ttaattgaat agctaaggtt tgcgcggtaa acttacatca ttaaaaaatt ctatgatggt
    40141 ttatataatg aatgctttcg atataaagtc gacaaagatg gacgtattgt ctatatcttt
    40201 gcatacgtca gacttgtttg atttggaaga tgtgctggtc aaattgggca agaagtttca
    40261 agagtctggt gttgttccat ttgtgctgga tgttcaagag tttgattatc ccgagtcttt
    40321 ggatcttgct gcattggttt cgttgttttc aaggcatggt atgcaaattt tgggtctgaa
    40381 gcattctaat gaacgttggg ctgctgcggc tatgaagtat catttgctgt tttgtctgtc
    40441 tcattcggaa aatgttaaag aactgggtca ggttgaggtg cagaaaacgg aggatggtca
    40501 gaaagcaagg aaaacagtat tgattacatc ccctgtccgt accggtcagc aggtttatgc
    40561 cgaagatggc gatttgattg ttacgggggc ggtcagccag ggggcggaat tgattgcgga
    40621 tggcaatata catatttatg cgccgatgag ggggcgtgct ttggccggtg ccaagggtga
    40681 tacttctgcc cgcatattta tccactccat gcaggcagaa ctggtttctg tggcgggtat
    40741 ttaccgtaat tttgaacagg atttgccgaa ccatctgcac aagcagccgg tacagatatt
    40801 gttgcaggat aaccgattgg ttatcagtgc aattggctca gagtaattgt ttgatattta
    40861 aaaaggaaat attgtggcaa aaattattgt agtaacttca ggtaagggcg gtgtcggtaa
    40921 aacgactacc agtgccagta ttgcgacagg tttggcatta cgcggatata aaactgcggt
    40981 aattgatttt gatgtgggtt tgcgtaacct cgacctcatt atgggttgcg agcgtcgtgt
    41041 cgtttatgac ctgatcaatg tcattcaggg ggaggcgacg ctcaaccaag ctttgattaa
    41101 agataaaaat tgtgaaaacc tgtttatttt gccggcttcc cagactcggg ataaagacgc
    41161 tttgacacgc gagggcgtag aaaaagtgat gcaggagctg tccggcaaga aaatgggctt
    41221 tgagtatatt atttgcgact ctcctgccgg tattgagcag ggtgcattga tggcgttgta
    41281 ttttgctgat gaagccattg taacgaccaa tcctgaggtt tccagtgtgc gtgactccga
    41341 caggattttg ggaattttgc aaagcaaatc ccataaggca gagcaaggcg gttcggttaa
    41401 agaacatctg ttgattacgc gttattctcc cgaacgtgtg gcaaaaggcg aaatgctgtc
    41461 tgtacaggat atttgcgata ttctgcatat tcctttgctg ggtgtgattc ctgaatccca
    41521 aaacgtcttg caggcatcca attccggaga accggtcatc catcaggaca gcgtggcggc
    41581 ttccgaggca tataaggacg ttattgcccg tcttttgggc gagaaccgtg aaatgcgttt
    41641 cttggaagct gagaaaaaaa gcttcttcaa acgtctgttt ggaggataag gtatgtcatt
    41701 aatcgaattt ttattcggca gaaagcagaa aacggcaacc gttgcccgcg accgccttca
    41761 aatcatcatt gcccaagagc gcgcccaaga aggtcaggct ccggattacc tgccgacttt
    41821 acgtaaagag ttgatggaag tcctgtccaa atatgtgaat gtttcattag acaatatccg
    41881 tatttcccag gaaaaacagg atggtatgga tgtgcttgag ttgaacatta ctttgccgga
    41941 acagaaaaag gtataggaca tgaccttaac cgaattgcgg tacatcgtcg cagtcgccca
    42001 agaacgtcat ttcggcaggg cggcgcggcg ttgttttgtc agccagccca ctttgtctat
    42061 tgccattaag aaattggaag aagagcttgc cgtctctttg tttgaccgga gcagtaacga
    42121 tattattacg accgaggcgg gggaacgtat cgttgcacag gcgcgtaagg tattggaaga
    42181 ggcggagctt atcaggcatt tggcaaatga agaacaaaac gagctggagg gtgcgttcaa
    42241 actcgggctg atttttacgg ttgcgccgta cctgctgccg aaactgattg tttcgttgcg
    42301 ccgtactgca ccgaaaatgc ctttgatgtt ggaagagaat tacacgcata ctttgaccga
    42361 gtcgctcaaa cgcggggacg ttgatgcgat tatcgttgcc gaaccgtttc aagagccggg
    42421 cattgttacc gaacccttgt atgacgaacc gtttttcgtg attgtcccga aagggcattc
    42481 atttgaggaa ctggatgccg tttcgccacg gatgctgggt gaggagcagg ttttgctgct
    42541 gacggaaggc aactgtatgc gggatcaggt actctcaagc tgttccgaat tggcggcgaa
    42601 acaacgtata caggggttga ccaatacatt gcagggcagc tcgattaata caatccgcca
    42661 tatggttgcc agcggtttgg caatcagcgt gttaccggca accgcgctga ccgaaaacga
    42721 tcatatgctg ttcagcatta ttccgtttga gggtacgccg ccaagccggc gggtcgtatt
    42781 ggcgtaccgc cgcaattttg tccgtccgaa ggcgttgtcg gcgatgaagg cggcgattat
    42841 gcagtcgcag cttcacgggg taagttttat ccgcgactag gcgcaggcat tgttttcaaa
    42901 acgccatttc cctgagccga caacacggta tgccaagata ttgccgtcat catcgatttt
    42961 gagtatagca tcgccacgga aactgccgtc ctgaagatat tcgacttttg catcactgtg
    43021 aatgttttca tcagtgccga tgcaatgcca tgtatagtgg attaacaaaa accagtacgg
    43081 cgttgcctcg ccttgccgta ctattgccaa ggcgtaccag cctgatttgt tgatgtgtgt
    43141 actactgcat gggttgttaa gccctgcaag tgatttaagt gtgaagattg agtaatgccc
    43201 actagtcgca ggcgcggggg gcaggtgcta agtcattggt tatctcctta ctatctatct
    43261 aactacaggg tgtattatgc gcctccttgt tgttttgtgc aattggtttt ctgataattt
    43321 atgtaaagat ttggtaaaga cgtatagcct ttttctttgc attgtgcttg cgcagggtat
    43381 caggtaggtc tcaaaaaatt tgaaaatttt tatgttttcg tataaaatct tttgcactta
    43441 tacggagtga gttatgcggt acaccattaa aacatttcaa acccttgctg atgaaacttt
    43501 cccctctaag aacttcaaaa ttctttcgtt caatggggtg gcacgaccta taaccattgt
    43561 ttgccctaca cacggggtgc agactgtttc tgcggggcaa gcatttatcc gttccaaaca
    43621 cggctgtcct gcctgtggtt tagttttatc aacctccgtt ttggcctcta ggggtcggtc
    43681 agtctccatc ctcgacaccg ccacaggcga aacactctcg ttccccagcg tacaagctgc
    43741 cgccaaagcc ctaaacacct cctacggctc tatacgaacc aagctcgacg gacgttcgtc
    43801 ccccgacaac cttgtctgta acaggtacaa agtaatgccg taatctcgaa acttcgtgct
    43861 ataataaact cctagctttt gcaggagttt gttttttatg gatttaaatg tcgaaatcaa
    43921 gaattttggc aaggttgccc atgctagggt ttccattcgc ccgcttaccg tcatcacggg
    43981 ttgcaaccca cactcgcgtt ccccagcgtt actgccgcag gcactgcttt gggtgtgcat
    44041 tttcagcaaa ttaaccacag gctcaagggt cgaacgtcct gtcaggaggt gttgatttct
    44101 acaccgatta tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
    44161 aaagaggccg tctgaaaaac atttttcaga cggccttgtt tattcaatca aatcagtctt
    44221 tcaacttcgc caactgattt tgaacttttg ccattttgtc ttccaattcc gccaaatcgg
    44281 ctttgtcttt ttccaccaga tgcgcagggg ctttttcggt gtagccgggt ttggagagtt
    44341 tggcgttgag tttgtccaag gctttttgca gcttctcggc ttctttgctc aaacgggcgg
    44401 tttcggcggc tttgtcgatt tcgactttca acatcaggcg cgcgccgttg cagacggcga
    44461 cgggcgcgtc ttcgctttcg ggtagggcgg cgacttgctg cgcttcggtc aggcgggtca
    44521 tcatcggcag gtatttgagg tagtctgcca agtcgtccgc gctttcgaca aacagcggag
    44581 ctttgacatt aggctggata cccatttcgc cgcgcaggtt gcggactgcg ccaatcaaat
    44641 cctgcaatac ggtcatttgc tcgaataccg tctgaacaat atcgccgccg tcggcttcgg
    44701 ggaagcgggc gagcatgatg ctgtcggcgt ttttcgcgtc gcacatagga gcgacggttt
    44761 gccacagttc ttcggtaatg aacgggataa tcgggtgcag caggcgcagg gcggcttcga
    44821 gtacgcgcaa taaggtatgg cgtgtggcgc gttggcggct ggcgcagccg gtttgaagct
    44881 gcactttggc aagttccaga taccagtcgc aatagtcgtt ccacacgaag ctgtacaggg
    44941 tttccgccgc caaatcaaag cggtaggttt cgtaggcttg cgttacttgc tcgatggtct
    45001 gattcagacg gcctacaatc cacatatcgg ggaaggagta gccgcgcggt tcggcagcgg
    45061 ttgcgccgta accgcagtct tggttttcgg tgttcatcaa gacgaagttg gtggcgttcc
    45121 agattttgtt gcagaagttg cggtagcctt cggcgcgttt gaagtcgaag ttgaccgaac
    45181 gccccaagct ggcgtagctc gccatagtga agcgcaaagc gtccgcgccc atactcggaa
    45241 tgccttcggg gaagagtttt ttcgtggctt cttccacttt cggcgcggtt tcgggtttgc
    45301 gcaggccggt ggtgcgtttt accagcagtt tttccaagcc gatgccgtcg atcaaatcca
    45361 cagggtcaat gacgttgcct tcggatttgg acattttttt gccttcgtgg tcgcgcacga
    45421 tgccgtggat atacacgtct ttaaacggta ctctgccggt gaagtgggtg gtcatcataa
    45481 tcatacgcgc cacccagaag aagatgattt cgtagccggt aaccaagacg ttggacggca
    45541 ggaaggcttt gagttcgtcg gtttcagacg gccagccgag cgtggagaac ggcacgagcg
    45601 cggaggagaa ccaagtgtcc aatacgtctt cttcgcgggt caagcctgtt ttgccggctt
    45661 gtttttcggc ttccacctga ttgcgggcga cgtaaacatt gcctgcttcg tcgtaccacg
    45721 cggggatttg atgcccccac cacagttggc gcgagatgca ccagtcttgg atgttgttca
    45781 tccattggtt gtaggtgttg acccagtttt cggggataaa gcgtaccgcg ccgctgtcga
    45841 cggctttttt ggctttgtcg gcgaggctca agcctttgaa ttcgctgtca ggctcgccgc
    45901 cgtttggcgt agcggacatg gcgacaaacc attggctggt cagcatcggc tcaatcactg
    45961 agcctgttcg gtcgcctttc ggcgtcatca gcgtgtgcgc tttgatttcg accaagaaac
    46021 cttgttcctg caaatcggca accatttgtt tgcgcgcggc aaagcggtct aagcctgcgt
    46081 atttttcagg cagggcaaag cttggttgcg cttcgccttt gaagttgaac acttcggcgt
    46141 ttgccaatac tttggcttcc aagtcgaaca cattaaccaa gcgcgtatcg tggcgtttgc
    46201 cgacttcgta gtcgttgaag tcgtgcgcag gcgtgatttt cacgcagccg gtgccgaagt
    46261 ctttttcaac gtattcgtcg gcaatcacgg gaatggtgcg gccggtcagc ggcaggatta
    46321 attctttgcc gattaagtgg gtataacgtt cgtcttcggg attgacggca acggcaacgt
    46381 cgcccaacag cgtttcggga cgggtggtgg cgacgatgac ggcttcggca ggattgtccg
    46441 ccagcggata gcggatgtgc cacatagagc cttgttcttc cacgctttcc acttccaaat
    46501 cagacactgc ggtacccaat accggatccc agttcaccaa gcgtttgccg cgataaatca
    46561 agccctgttc atacaggcgt acgaacactt cggttacggt ttcggcgcgt acatcgtcca
    46621 ttgtgaaata ctcgcgcgtc cagtcggcag aacagcccac acggcgcatc tgctgcgtaa
    46681 tcgtgccgcc ggaaacttct ttccattccc acactttttc caagaatttt tcacgatcca
    46741 agttatgacg ggacacgttt tgcgcggcaa gctgacgctc gaccacgatt tgcgtggcga
    46801 tgcccgcgtg gtcggtgccg ggaatccagg cggtgttgca gcctttcata cggtagtagc
    46861 gggtcaggcc gtccataatg gtttggttga aggcatgacc catgtgcagc gtgccggtta
    46921 cattgggcgg cggcagttgg atggaaaaag acggtttggt caaatccata tcaggttgga
    46981 aatagccttg gctttcccag ttttgatagt gcttggattc gatttcggcg ggggagtatt
    47041 tgtctaacat gatggaactt tgtgaaatta aggttatttt tgatgtgcgg attataacgc
    47101 aaaaaggccg tctgaatcat ttcagacggc ctttggcata caggttttaa aaatggaaca
    47161 ataccaggct gacggcaatc accgccatgc ccattgtcag gccgtaaacg gtttcatggc
    47221 cgtctgaata gcgtttggca gccggcagca gctcgtccaa cgccaaaaac accatcacac
    47281 cggctatcac gccgaatacc gaaccaaaca cggcaggcga caaaaacggc tgcaaaacca
    47341 aatagcccaa agccgccccc aacggctcgg ccaagccgga tagcagacac gcccacaccg
    47401 ttttcttacg gctgcgggtg gcaaaataaa ccggcgcggc gatggaaatg ccctccggaa
    47461 tattatggat ggcaatcgcc aaggccaaag gcatcccgac tgctggattt tccaatgtgg
    47521 caaaaaacgt cgccaagcct tcggggaaat tgtgcgcagt aatcgcaaac gccgccatca
    47581 tgccgactcg cgcgatatgg cggcgtttgc tttcttgaaa cgacgggtct tgcgcgtcta
    47641 aagtttcatg cgggttcggc accagacggt caatcagcgc aatgccgccc atcccggcca
    47701 aaaatgccat ggtcgccgcc gcaaacgcgt ggtctttatc ataaatttca gcgaacgcct
    47761 cgctggattt actgaaaatc tccgtcaggg aaacatatac catcgcaccg ccggcaaacg
    47821 ccaaaccaaa cgacaacaca cgcggattgg gcgttttgga aaacatcacc aagccactgc
    47881 ctaatacggt aaacaaaccg gcagccaatg tgatggaaaa ggcaacggcc aaattggaca
    47941 tcgaaaaatc gggcatgaga aaacctgcgc taaaagctgg gacaggttca gactaacact
    48001 ttttaatgta tatgataata gttattattt attttattga ttggatacac ggattttgaa
    48061 acaaaaggcc gtctgaaaaa tgattttcag acggccttta aatttgaaat gccgctaaac
    48121 cttatcgctt tccagcttaa gcctgataac gcgacaggct caaatcgtcg ctgcggattt
    48181 cggtgtcttt gccgctcacg atatcggcgg ttaattttgc cgaacccagc gacatggtcc
    48241 agcctaaagt accgtggccg gtattcagaa acaggttgtc aaagcgggtg cgaccgatta
    48301 acggcgtgct gtcgggcgtc atcggtctga ggccgctcca gaacgatgct tggctcaaat
    48361 cgccgccttc cgggaacaag tcgttgacga ccaaagccaa ggtttcgcgg cgtttttcgg
    48421 gcagtttgat ttcgtagccc gacaattccg ccataccgcc gacgcggatt ctgttgtcaa
    48481 agcgcgtgat ggcgactttg tagctttcat ctaaaacggt ggacaccggt gcgccgtcgg
    48541 aatttgtgac cggcagggtc aaggaatagc ctttgacggg ataaatgggc agattgagat
    48601 ccaactgcgc caaaaccgtc ctgctgaaac aaccgagcgc gcagacaacg gcatctgctt
    48661 caaaccgccc tgtttcggtt tcaacggttt tgatgcgcag cccgttgtgg tcgatgcggc
    48721 tgatggtttg gttgaaatga aaccgtacgc ccttttcctg acacaatttg tataggtttt
    48781 cagtgaagag gcggcagtcg ccggttgcat ccgcaggcag gtgcaggccg ccggcaattt
    48841 tggcggtaac gcgtgccagc gcaggctcga attctgcgca ttcttcgggc ttcagacggc
    48901 ggtacggcac gccgtagcgt tccaaaacgg caatgtcttg ttctgcagct tcaacttctt
    48961 tagcctgacg gaaaatctgc aacgtccctt ttttgcgccc ttcaaaattc atgccggttt
    49021 gcgcttcaaa acggcggaac atttcacggc tgtattcgga aatcctgacc atgcgctctt
    49081 tattggtttg atagtgcgct gccgtgcagt tttgcagcat ccgccacagc cattcgattt
    49141 gatacaggct gccgtcgggg cgaaacagca aaggcggatg gcttttaaac agccatttca
    49201 gtgctttggt cggaataccg ggtgcagccc aaggcgtggt atagccgtaa gaaagctggc
    49261 ctgcattggc aaaactggtt tccatcgcca cacccttggc gcggtcgatg accgttactt
    49321 catgtccggc ctctgccaga taccacgcgg aagacacgcc ggcaacaccc gcacctaaaa
    49381 caagcacttt catgtttctc cctccgactt tttcaaaaca gacttaatat gccgtgccgt
    49441 ctgaatattc ggattcagac ggcctcggat attaatgcgg caattcgccg tttgtgattt
    49501 tttgtttgaa gtcgcgcgtt tcattgacga tgactttcgc catcaataaa agtgcaatca
    49561 ggttgggcaa tgccatcaag ccgttgaatg tgtccgaagc cagccacacc aaatcaaggc
    49621 tcaacacggt acccagcata acggaagaaa cataaccgac gcggtacaaa ccggcaaatt
    49681 tctcgccgaa aacatacacc gcgcattttt cgccgtaata gcaccagccc aaaatggttg
    49741 agtaggcaaa gaaaatcagg ccgatggtaa caatccagcc gccgatgccg ggcagcattt
    49801 tttggaatgt gacggttgtc agtgccgcgc cgctcacttc aggtttgaca aactcgccgc
    49861 ccgcgccgag cagtcccatt accaacacga tgccggtaat cgagcaaacg acgatggtat
    49921 ccaaaaacgt accggtcata gaaaccaagg cctgacggac gggatggtcg gttttcgcgg
    49981 ctgcggcggc aatcggtgca gaacccatgc ccgcctcatt ggagaacacg ccgcgcgcca
    50041 cgccgtagcg gatgaccgta ccgatagcgc cgcccgccac tgcctgcgcg ctgaacgcat
    50101 cggagaaaat cagcttgacg gcaggcatca gtgcatcgga attaatcgcg ataatggaaa
    50161 gaccgcccaa cacataaaac accgccatag caggcacgat gaaagaagcg gctttggcga
    50221 tgcctttaat accacctaaa acgacaacgg cagtcagaac ggtcaacgta atgccggtat
    50281 aggcaggttc gataccgaag ctggtttgca ccgcctgtgc aaccgagttg gactgcaccg
    50341 agctgccgat accgaaggaa gcgaatgtgc cgaacagcgc aaacgcgacg gccatccatt
    50401 tccagttttt gcccaagcct ttttcgatgt aatacatcgg gccgccggac atttcgcctt
    50461 tggaattgtt gacgcggtat ttcaccgcca acacgccttc gccgtatttg gtggccatgc
    50521 cgaaaatggc ggtcatccac atccaaaata ccgcgcccgg gccgccggtt accaccgcag
    50581 tcgccacgcc ggcgatgtta cccgtaccga tggtggcgga cagcgcggtc atcaacgccg
    50641 cgaaatggga aatatcgcct tcgtggcctt cgccgccttc atacttcttt gacggcacaa
    50701 atgcctgttt cagcgcatag cccaacatag tgaactgcaa gcctttcagc ataacggtca
    50761 gcacaatgcc ggtgccgacc agcagcatca acataacaga tccccagacc cagccgctga
    50821 cggtttcgaa aaaggctttg agattgtcta aaaacaattg catggctttc tcctttgtct
    50881 gttttatttt taaaacacca cttttgtagt gtcctataat ttcagcacag aatatccaat
    50941 aagacaatat gttcttttga aaaatacttt tggttttttc gccgaaagca ggccggttca
    51001 agctgcggaa attgtttgca attctttaaa agcagcggcg gaggtcacaa tgaaatgtcc
    51061 gaatggggat gtggtcggcg gcagaaatca tcaatgctgc cgactgccat acttctgaaa
    51121 tctacaaaat gatgcatcga tcaaacaata taccgcttta aaaaaaccga tgccgtctga
    51181 aacgctttcg gggtttcaga cggcatcaaa agggtacggt cagcggatga tgccgcgcgt
    51241 cgattgtgtg aaaaagtctc ggaatacggc aagctcggct tgggtttcgg cgcggcggag
    51301 gatgtctgcc ttggcctctt caaacggaat gccgcgatgg tagagggttt tgtacacgtc
    51361 tttgacggcg gaaatctgtt ctgcggtaaa accgttgcgg cgcatgcctt cgctgttgag
    51421 ccccgccggt tcggcgcggt agcccgatgc cataaagtag ggcggtacgt ctttgtgtac
    51481 gcctgcggcg aaggcggtca tagcgtagtc gccgatgtgg cagaattgga aaaccagcgt
    51541 gtagccgccc aaaacgacgt agtcgccgat ggtaacgtgt ccggcaagcg aggcgttgtt
    51601 ggcgaagatg gtgtggttgc cgaccacgca gtcgtgcgcg aggtggcagt acgccataat
    51661 ccagttgtcg tcgccgatac gggtttcgcc gatgccggtt accgtgccta aattaaaggt
    51721 ggtgaattcg cggatggtgt tgccgttgcc gataatcagc ttggtcggct cgtcgcggta
    51781 ttttttgtcc tgcgggattt cgccgaggct ggcaaattgg aaaatgcggt tgttttcgcc
    51841 gatgctggtg tggccgttga tgacggcgtg cgggccgatt tcggtattcg cgccgatttg
    51901 gacgttgggg ccgataacag tgtacgcgcc gactttgacg ctggagtcga gttcggcttt
    51961 ggggtcgatg acggcggtcg ggtggatgag ggtcatgttt tgaatactcc gctgcttgtt
    52021 aagacaaata atagaaaatt acagtttgtc gatttcatcg cagaccgcaa tcacttgatc
    52081 gctaaaatgg ctaaatactg aagcagtatc tacatccatg taattaagca gttcaaaaat
    52141 agattggtga atatttctag cgtagttggt atcttttatt gcacctttaa aacaaatcgg
    52201 ctcgtgatgt gccaaacgat tacggaattt gttaatattg gaaagttctc gaaaaatcca
    52261 tttttggtta tattgcacac ttggtgtaga tttgggcttt tttggaaaaa ctttcatcaa
    52321 tactttccct gtagcatcaa attgggcatc tttccctcct gcaaacagat accgccaaaa
    52381 accgaatcct agtcctgcaa ccaattggtt gtggtcaaac tttcccccgc tattattttg
    52441 tattttagat aatgcaacct tgattaattc agcagattct ttcgtgccat ttcttaaaaa
    52501 gcaaccttga tattttaatg cgggattcgt ctgtggctgt atgctgttat ataaccagtt
    52561 tctgtcttta aacgcctgct gaaggcaaat gtcaattttg tttcttaaaa caatctcaaa
    52621 tattgaaaca acatgaaaaa gttctcttga taaagataaa ttcaataaat acaattcagc
    52681 tgctttgtct tccccaacgg catttttata ccgttccatt ctgaaatcag aaaaaatact
    52741 acagcattcc ttataatcca cgctaacacc tgattcttta tttttaaatt gacgtttcaa
    52801 actatatttg atacagttag gtaaaactaa tccccaccgt gtccctgggt ttgaataaac
    52861 ctaaaccggt gggttttctt tttattaggt cgtctgaaac tttcagacga cctttttccg
    52921 aacgctcaaa ccacgcgttt ggcgcacata atcacggctt caacggcaac ttgcccgtcc
    52981 actttggcga cggcgttgaa tttgccgatg ccgcgccggc tggtcagcag ctcgacttca
    53041 aagacgagtt ggtcgccggg gatgacttgg cgtttgaaac gggcttcgtc tatgccggcg
    53101 aagaagaaga attcgttttc tttgcgcccg ccttcgctca aaatcgccaa cgtgccgcag
    53161 gcttgcgcca tcgcttcgat gatgagtacg ccgggcatca cgggcaggtc gggaaagtgg
    53221 ccttggaact ggggctcgtt tatgctgacg tttttaatcg cggtcagggt tttcatcggc
    53281 tcgaaggcgg tgatgcggtc gagctggaga aacggatagc ggtgggggat gagtttttgg
    53341 atgtctttgg cttcgatggg gagttgtacg tccatgtctg tcgtattcct tgaataaaat
    53401 cggtttggat ttattgcgtg tctttgccgt ctgaaagctg ctgttccagc gttttgaggc
    53461 gtttgttcat ttcgcttaag cggtggatat ggacggcgtt gcgcgcccat tctttatggg
    53521 tggacatcgg gaagatgccg gcgaggtgtt tgccgctttc ggtaatgctg tgggtgacgg
    53581 acgtgccgcc gccgatggtg gttttgtcgg cgatttcgat gtgtccgacc gtaccgacgc
    53641 cgccgccgat gatgcagtag ctgcctatgg ttacgctgcc tgagatgccg gttttggcgg
    53701 cgatgacggt gtgcgaaccg attttgcagt tgtgtccgat ttggacttgg ttgtcgattt
    53761 tggtgccgtt gccgacggtg gtgtcgctca tcgcgccgcg gtcgatgttg gtgttcgagc
    53821 cgatttctac gtcgtcgccc agcgttaccg cgccggtttg cgggatttta aaccatgaat
    53881 cgccggcaaa agcgagtccg aaaccgtccg cgccgatgac cgcgccgctg tggatttcga
    53941 cgcgtctgcc cagtgtgcag ccgtaataaa cgacggcgtt gggatgcagg acgacttcgt
    54001 cgcccagttt gcaatcgtgt tggacgacgg cgtttgccaa gatgcggcag ccttcgccga
    54061 gcacggtgtt tgcgccgatg tagacgttcg cgccgatttc gcagctggcg ggaacggtcg
    54121 cgcccggttc gacgacggcg gtcggatgga tgccgccgcg cgctttgacg acgggtgaaa
    54181 acaggcgggc gactttggcg aaatagagat aggggtcgtc ggcgacaatc aggttgcgcc
    54241 attcaaatcc gtctgccgct ttggcggaaa cgatgaccgc gcccgcgctg ctgtcgtgga
    54301 cttcggcttt gtatttcgga ttggcaagga agctgatgtg ttccgcctgc gcgtctgcga
    54361 gcgggcgcac ggcggtaacg gaaatgtcct cgccgcgcca ttcgccgccg agccgcgcgg
    54421 tgatttggga cagggtgtag gtggccggaa tcatggtttt cctgttcggt atgccgtctg
    54481 aaagggtcag cgggcgttca tttctttaat gacgctgtca gtaacgtcgt attgggtgtt
    54541 gacgtaaatc acgttctgca aaatgacatc gtaaccttcc tgtttggcga ttttgacgat
    54601 gacgcggttg gcgttttgct ggagggaggc aaactcttcg ttgcggcgga ggttgtagtc
    54661 ttcttcaaac tgcgcctgtt ttttgcggaa cgctgcgacc agcccgcgcc atttttcttc
    54721 ggcttgcgcc ttttttgcgt ttctgagttt gccttcggca agctgccttt ccaaatccag
    54781 accttcgcgt tgcagttttt gcaattcgtc ctgacgggcg gaaaattcgc tgtccagcgt
    54841 tttttgaatc ttgcgcgcct gcttggattc gaggtagatg cgctcggtgt tgataaagcc
    54901 gattttttgg aaggtgtcgg cgtgcgcgcc tgcggtgcag cacaaaccga tcagagccgc
    54961 ggcaaacgcg cgggtcaaac gggtcatggt aaaactcctt cgaatgttgc cgcgaaatgc
    55021 cgtctgaagg gcttcagacg gcatttgcgg gattagaacg tcgtgccgag ttggaattgg
    55081 aagcgttgga tttcgtcttc cggttttttc ttcagcgggt aggcgtagct gaatttcatc
    55141 gggcctaaag gcgagagcca ggtaaccgcg ccgccggcgg aatagcgcaa ttcgttggta
    55201 aaggtggatt tatgggtatt gccggcgccg taaatgtttt gaaccctgcc gccggtcgcg
    55261 gaactgctgt tgtcgtcgta ggttttgccg tcccacacgc tgcctgcgtc ggcaaacagg
    55321 ctcaggcgga cggtgcgcgc gtctttcgcg ccaggcatcg ggaagagcag ctcggcggag
    55381 acgttggctt ttttgttgcc gccgtagctg attttttcgc cgtattcgtc atacactttc
    55441 ggaccgagcg tgccgctttc gtatccgcgc accgaaccca ggccgccgcc gtagaagttt
    55501 tcaaagaagg ggatttcttt ggttctgccg tagccgcccg caatgccgac ttcgccgccg
    55561 agcatcagcg tgaaggtttt gcttaagggg aagaaccagg tttggttgtg ggtggcggag
    55621 tagtattgca gtttgctgcc gggcagggcg atttcggcgt tcacgcccgt caggtagccg
    55681 cgcgtcggcc ataacgcgct gtcggttttg ttgcgccccc agccgacggt acctttgtac
    55741 agccagcctt tgaagctgcc gtctgtgccg tcggttttgc cgtatttctt gataaagtcg
    55801 gcatagtgtt tgggcgcttt gttgtaggtg ttgacggtca ggtgttctgc caccaaaccg
    55861 aaattcacgc ggtcgtattc ggtaacaggc acgctcatgc ggatgcctgc gcctgccgtg
    55921 gtggttttat attgtttgat gctggtcgat gctttgcgcg ggtcgaaggc ttttccgtaa
    55981 acatcgtagc ccaggctgac cccgtctgcc gtgaagtacg ggtcggtaaa cgacagcgag
    56041 ccgttgagcg tggttttgct tcgtgaggcg cgcagggcgg ccgacttgcc cgtaccgaac
    56101 aggttgtctt gggaaacgcc tgcggacatg accaggccgg tatcctgtac ccagcccgcg
    56161 ctcaagtcga gcgagccggt ggaacgttcg gtcaggctca tgttcaaatc gactttgtcg
    56221 ggtgtgccgg caagcgggac ggcgtcaaac tggacattgt cgaagtagcc caaaagctcg
    56281 acgcgctctt tggagcgttg cagcttggag gtgtcgtaag gcgcggattc catttggcgc
    56341 aattcgcggc gcacgacttc gtcgcgagtt ttgttgttgc cggtgatgtg gatttcgttg
    56401 acgtagattt tccggcccgg ttcgatgtgc aggacgaaat cgacggtttt ggtttcggcg
    56461 tttggcagcg gctgtacgct gatttcgctg tatgcgtagc ctgccgagcc catgcggttc
    56521 tgaatctcac ccaaaacggc ggtcatctgc tggcgttcgt accatttgcc gggcttcatg
    56581 gtcagcagct tttccagttc ggctttgggg acttcgttgg tgtcgccttc gatggagact
    56641 ttgccccaac ggaaacgtcc gccttcgtgg acggtgattt tgatggtctg cttggttttg
    56701 tcttcgttgg tttggatgtc ggtatcgagg atgcggaaat cgaagtagcc gttgttctgg
    56761 tagaagtcgg ttactttttc catgtcttgg gcaaatttct gctcgttgaa ttggttgctt
    56821 cgtgtcagcc atgtccaaat gccgccttcg gtcagcgaca tctgccgcat cagtttgcgg
    56881 tcggaataga cttggttgcc ttcaaattcg atgtcggtga ttttggcgga tttgccctcg
    56941 tcaatcgtga tgtcgatgtc gacgcggttg cgggcgagtt tggttacttt gggcgtgatt
    57001 tggatattga gtttgccgcg cccgaggtat tcttctttca ggccggcgac tgcctgattg
    57061 agtgtcgcct gattaaagta ttgcgactgc gccagcccga acgattcgag gtttttctta
    57121 atggcatcgt tttgcagcat ttttgcgccg gtgatattga gcgatccgat ggtggggcgt
    57181 tcgatgacgg tcagcaggag ctgcccgtcc gcagtctcga cgcgtacgtc gtcaaagaaa
    57241 ccggtggcgt acaggctttt gatgatggca ctgccgtgtg tgtcgttgta ggtgtcgccg
    57301 attttgacgg gcaggtagtt gaatacggtg cttggctcgg tacgctgcaa gccttcgacg
    57361 cggatgtctt ggatggtgaa gtcggcaaat gccaaaggcg atatgcccaa catcatcagt
    57421 gcggaagcaa tctgtttcag tttcatcgta agttccttgt ggtgcggaat gcggtttcag
    57481 acggcattcc gaaacgtaaa atctaaccga gcagccgggt aacgtcgttg aagaaggcga
    57541 ccgccatcat cagcatcatg agggcaagcc cgaagcgcaa accgatgttt tggacgcgtt
    57601 cgcccaaagg tttgccgcgt atccattcgg cagtataaaa cacgaggtgg ccgccgtcca
    57661 aaacggggac gggcagtagg ttcagcacgc cgaggctgat gctgaccagt gccaaaaatt
    57721 ccaaataact ttgcaagccg agttcggcgg actgtccggc aatgtcggca atggtcagcg
    57781 gcccggaaat atggctgacg gaggcgttgc cgctgattag tttgccgaaa aatttgaggg
    57841 ttgtccacga gtgggaaacg gttttttccc agcccatgcc gaatgcgcgg ataacagacg
    57901 gacggtagct gcggcggatt tgcgcgtccc acgccctgtc cggctgcgga cggaggccga
    57961 cgcgcccgat cagggtgtgg tcggactgtt cgacagtatc ggggcggatg tcggcggtat
    58021 gggtttgtcc ggcgcgttcg taggttaagg cgattttcct gccggggctt tggcgggtca
    58081 ggtttgccca ttcctgccac gaggcgatgg gtttgccgtc ggcggcagtc agcctgtcgt
    58141 ccggttgcag acctgctttg tcggcgggac tgcctttttc cacgccgccg acaacggttg
    58201 tgattttaaa gggcatcagt ccgatgtagc cttggttttt tgcgatttta ccggcttccg
    58261 gcgtgcctgc ggcatcgatg gtgcggacgg tttgcgcgcc cgatgccgtc tgaacgccga
    58321 cggcgacttt gcctgcctca aggttgagga cgatttcggt ttgcgcgctg ccccaatctg
    58381 caacgggtgt gccgttgacg gattgtattt tgtcgccgct ttggaagccg gcgcgggcgg
    58441 caatggtgtc aggttcgact gtgccgacgt aggggcgcag ttcggttacg ccgaaggaaa
    58501 agctcagtcc gtacagcaaa accgccagtg cgaggttggt cagtgggccg gcggcgacga
    58561 tggcgatgcg cttggcgggg tgttgtttgt caaaagcgta gggtaaatcg gcttctgata
    58621 cttcgccttc gcgcgtgtcg accattttga cgtaaccgcc caacggaatc ggggcgaggc
    58681 accattcggt gtcgccgcgc tttcgggtga aaaacggttt gccgaagccg acggaaaagc
    58741 gtacgacttt gacgccgcac aatctggcaa cgatgtagtg tccgaactcg tgcaggctga
    58801 ccaaaatcag gatggcgaag ataaaagcta gaagggtgtg caaatggttt tcctttgata
    58861 acggtgttcg gacggcatca gcgcagtgtg ccgataaatg cccgcgcttg tgcgcgtgtc
    58921 cgggcatctt gcgccaagag cccccctatg tcgcctatgc cgtctgaaaa gtcttgtgaa
    58981 agacaatggg cgacggtttt ggcaatgtcg gtaaacttaa tctgtccgtc caaaaaggcg
    59041 gcgacggcgg cttcgttggc ggcgttcaat acgcagggcg cggctccgcc tgcgttcatg
    59101 gcttcatagg cgagcttcag gcaggggaag cggtcaaagt cgggcttttg gaaggtcagc
    59161 gcggacaatg cgtcgaaatc aaggtcgccg acacccgaat cgatgcgctc gggcaagccc
    59221 aaacaatagg cgatgggcgt tcgcatatcg ggattgccca gttgtgccaa cacggagccg
    59281 tcgcggtagc gcaccatgct gtgtatcacg gattgcggat ggatgacgac ttcgagcttg
    59341 tcaggcggac agttgaacag ccaatgcgct tcaatcagct ccaaaccttt gttcatcatg
    59401 gtggcggaat cgacggagat tttgcgcccc atgctccaat tggggtgttt gaccgcttgg
    59461 gcgggcgtaa tgctgtcgaa cgtgcctaaa tcggtcgtta aaaatgggcc gccggaagcg
    59521 gtcaggataa tcgaagcgat gccgtgttcg ttcagacgac ctgtgtaatc gcgcggcaaa
    59581 acttggaaaa cggcgttgtg ttcgctgtcg acgggtaaca ctgccgcgct gtttgcacgg
    59641 gcggtttcca taaacaacgc gccggaaacc accagcgtct ctttgttcgc cagataaatg
    59701 gttttgcctt tttgcgccgc tgcgagcgcg gaaggcagcc ccaccgcccc gacgatggcg
    59761 cacatgacac cgctgacttc gtcggcagag gcaacgtcaa ccaatgcctg cgcgccgtgt
    59821 aaaacctgag tcgccgtgcc gtcgcgtttc aacagggctt caagccgggc ggcgtattcg
    59881 gcatcggcaa cgacggcata ttcgggttgg aacgtttgac attgagccgc cagtttctcg
    59941 acctgcttat gccctgccag cgcgaatacg cggaattttt cggggtggcg ggagacaacg
    60001 tccagcgtgc tttcacctat gctgccggta ctgcctaata tggtcaggac ttgtggtgtc
    60061 ataatgagga taactttata ccggatgccg tctgaagcgt tttcagacgg catagaatca
    60121 atttaaaacc gacatcatcg ctgcatagac gctgataacg gcaatcaggc tgtcggtacg
    60181 gtcgaacacg ccgccgtgtc cgggcagcag cttgctgctg tctttgatgc ctgccgcgcg
    60241 cttgagccag ctttccaaaa ggtcgccgca tacgctgaca acggtcagca ccaaaccgat
    60301 taacacggta tcgaaccagc ctgtatcgaa tgccagccag ccggcacttc gtacggcggt
    60361 catgtacact gccacgcaaa ccgcgccgcc gattgcacct tcccagcttt tgccggggct
    60421 gattgccggc gcgattttgt gtttgccgaa cgccttgccg ctgaaatacg cgcaaatatc
    60481 ggcaacccac accaaaccca tcacggcgag cagcggcagg gcatcatcgg gatgcgggcg
    60541 cagggatacg agcgcgaacc aaaacggcat gaccagaagc cagccgacgg cataaacctg
    60601 ccaaccgccg ttgagcctcc atttgaatct caaccataaa ggcataacgg cgagccaaaa
    60661 tgccaaaaca acataccaaa ccaaattagg cagcatccag ccgcccgcat aggcaaccac
    60721 gccgaaaacc aaggttgcgg cgaggtaatg gttggtttta attttgcaca aaccgcccat
    60781 acgggcatat tcccacaagg caatcagggc aatcagtccg caaaatgcag cccacaacca
    60841 ttgcggcgcg taaaacagca tgcccagcat cagcggcagc agccacatgg cggttattac
    60901 ccgttgtttc agcatattca gttcctttgc tgttcgatag gcagttgctc ggaggtgcgt
    60961 ccgaaccgcc gttcgcgttt ttggaacgaa gcgacggcat cgtccaaagc cttgccgtca
    61021 aaatcgggcc acaaaatatc ggtgaaatac agttctgcat atgccatctg ccagagcagg
    61081 aaattgctga tgcgcgtttc gccgccggtg cggatgaaca aatccggttc cggtgcatcg
    61141 cccagcatca agtgtttcgc cagcgtgtct tccgtaatct cggatacgcc ttcggcaatc
    61201 agtttgtttg ccgcctgcaa aatatcccag cggccgccgt aatcggcggc aatgctcagg
    61261 gtcaggccgg tattgtttgc cgtcaacgct tccgcctctt cgatgccttg cagaatctgc
    61321 cggttgaagc gttcgcggct gcccaatatc ttcaggcgca tattgttttc gtgcaggcgg
    61381 cgtacctgtt tttgcaaagc ctgtaaaaac agccccatca ggaacgaaac ttcgtcttcg
    61441 gggcggcgcc agttttcggt tgaaaaggca aacacggtca gatattgcac acccagtttg
    61501 gcgcaatgct tcaccatatt ttccaatgcg tccaaaccgc gtttgtgtcc cattatgcgc
    61561 gggaggaaac gttttttcgc ccaacggccg ttgccgtcca taatcacggc gatatgcttg
    61621 ggaatggcgg tgtgttccaa aacggcctgc gtgctgcttt tcatgtctgc ctttcgcggt
    61681 tcggcattca aatgccgtct gaacgccgaa ccgtgcaggt taaattgcca tcaaatcttc
    61741 ttctttggca gtcaggagtt tgtcggcttc ggtaatgtat ttgtcggtca gtttttgaac
    61801 cgcttcttcg ccgcgacgtg cctcgtcttc ggaaatttct ttgtctttga ggagtttttt
    61861 gatgtggtcg ttggcatcgc ggcgcacgtt gcggatagag acgcggcctt cttccgcttc
    61921 gccgcgtacg actttaatca ggtctttgcg gcgttcctcg gtcagcatgg gcatcggcac
    61981 gcggatcagg tcgccgacag ctgccgggtt cagtcccaag tttgaatcgc ggatggcttt
    62041 ctcgactttg gccgccatat tgccctcaaa cggtttcacg ccgatggtgc gcgcgtccag
    62101 aagcgttacg ttggcaactt ggctgacggg gaccatgctg ccccagtatt cgacttccac
    62161 ttggtcgagc aggccggtat gcgcgcggcc ggtacgcact ttcgccagat tttctttcag
    62221 tacttcgacc gaacgctgca tcttgccttc ggctgttttt tgaatatcgt tgatcatatt
    62281 gttctttcgg tgggataagg tgggcgggag accgtctgaa cgcgtttcaa gccgttcaga
    62341 cggcataaag accgttaacc gcgaatagta ccgttattcg ggcataacga caaggtaggc
    62401 ggattgggga tgccgtctga agcgacaggc gtttcagacg gcatcgtgtc cgaccgtcag
    62461 ccgtgttccc gtgtttcaag caggctttgg cgcaggtgtt ggcgttcgtg ggcatccagc
    62521 catttgcggc gggtgcgttg cagcaggatg acgagggcgg aaatttcctg acgcatattg
    62581 gtgctgagcc agaggaagcc ctgccattgg tagtggaggt gttcggcgag ggcttccagt
    62641 tcggggttga tggcggtgtc gatgcggatg cggcgggcgt gtctgccgtt gataagggcg
    62701 acggtttgtt gcaggtcggt ttggagcagt gtgaagtggc ggtcaagcag ccggatttcg
    62761 ctgccgttga gtttgggaga ttgcagcttg gcggcggtgg tcaggagcag ctcggtggtg
    62821 ttgacgattt tacggtgggc gtgctgcatg gcttccatca tggcggggct gatgcggctt
    62881 tcgcccgatg tggcggcgag atggctgcgg cttttgacca tgcgtgcgtt gatttggcgc
    62941 attttcgcca tgttctcctc gaggcgttcg cgggtcatgc gcctgccgtt gctgatttcg
    63001 gcaatcattt tgctgcagtc ggccaggttg tcggcaagca tgaaacgcca catcagtgtg
    63061 gatttcagcg gcagcagttt ggcggcggcg atggcgatgg ccgcgccgat gaggacgttc
    63121 atggcgcgca tgagtccgct gtcgagccat tcgctgccgt tgtcgccgat gagcatacac
    63181 atcgtcagcc ctgccagcat agggacgtag ccgtttttgc cgaccgccgc ccagccggcc
    63241 agtgcgcttg ccgtgccgac ggtgaggtag aagaggaggt tgccgtggaa ataatgctgg
    63301 ttcagccata aaacgcccaa acccgcgccc agcccgatga ccgtgccgag catacgttcc
    63361 accgccttgg agtaaatcgc cccttgaaac tggagcatgc cgaggacgac gaagacggtc
    63421 atccctatcc actcgccgtg ttggaggtgg agcagccggg cggaggcggt ggcgaacagg
    63481 acggccccgc cgagccggac ggaatggatg aggcggcggt agcggtagcg ttcgtaggag
    63541 ttgagccagc ggctgacgag gcggttgcgt tgcgaggtgt tcatatcggt tgtgccgtct
    63601 gaagcggaaa tgtgaaaaag cacaggcttc ccgaggaagg gagggtctgt gcttggtatt
    63661 ggtgccggag aagggaatcg aacccccgac cttcgcgtta cgaatgcgct gctctaccga
    63721 ctgagctaca ccggcgtttt ttcgtcatga tatatatgaa cggttgtttg tgcaactttt
    63781 cgggcgggcg gcaaggcagt gcgcggtata gtggattaa
//
LOCUS       DAWHRA010000002        31618 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWHRA010000002 DAWHRA010000000
VERSION     DAWHRA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.002"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(93..932)
                     /locus_tag="WIB30_000081"
     CDS             complement(93..932)
                     /locus_tag="WIB30_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ3796231.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1217..1292
                     /locus_tag="WIB30_000082"
     tRNA            1217..1292
                     /locus_tag="WIB30_000082"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Arg,seq:ccg)
     gene            complement(1357..2550)
                     /locus_tag="WIB30_000083"
     CDS             complement(1357..2550)
                     /locus_tag="WIB30_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ3796232.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNRALVQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="WIB30_000084"
     CDS             complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="WIB30_000084"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ3796233.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLAETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3802..4902
                     /gene="aroC"
                     /locus_tag="WIB30_000085"
     CDS             3802..4902
                     /gene="aroC"
                     /locus_tag="WIB30_000085"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ3796234.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLALTESDIQAD
                     LDRRKPGTSRHVTQRREADQIEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVRVNTPDITLSNK"
     gene            4994..5419
                     /locus_tag="WIB30_000086"
     CDS             4994..5419
                     /locus_tag="WIB30_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ3796235.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5493..7478)
                     /gene="parE"
                     /locus_tag="WIB30_000087"
     CDS             complement(5493..7478)
                     /gene="parE"
                     /locus_tag="WIB30_000087"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ3796236.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7545..8069)
                     /locus_tag="WIB30_000088"
     CDS             complement(7545..8069)
                     /locus_tag="WIB30_000088"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ3796237.1"
                     /translation="MLDREGYRPNVGIILINERNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQLSGNR"
     gene            8234..9529
                     /gene="serS"
                     /locus_tag="WIB30_000089"
     CDS             8234..9529
                     /gene="serS"
                     /locus_tag="WIB30_000089"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ3796238.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYEFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9602..10600)
                     /locus_tag="WIB30_000090"
     CDS             complement(9602..10600)
                     /locus_tag="WIB30_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ3796239.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVELNELYARSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSSAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10725..11801)
                     /gene="prfA"
                     /locus_tag="WIB30_000091"
     CDS             complement(10725..11801)
                     /gene="prfA"
                     /locus_tag="WIB30_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ3796240.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITNALIAEHQAELLAAMGD"
     gene            complement(11905..12684)
                     /locus_tag="WIB30_000092"
     CDS             complement(11905..12684)
                     /locus_tag="WIB30_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ3796241.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAAREAGVGRFIVPATRPQDW
                     QDVADLAQRLSENPLWGNIHIALGIHPWFSDDVSEPDFQLLEQTLKAHPTAWVGEIGL
                     DFYDKTQTPPQRERQIQVFVRQLEIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGI
                     VHAFSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLK
                     KEINTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(12799..13788)
                     /locus_tag="WIB30_000093"
     CDS             complement(12799..13788)
                     /locus_tag="WIB30_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ3796242.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGVIAEWDETQGWYNLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13855..14538)
                     /locus_tag="WIB30_000094"
     CDS             complement(13855..14538)
                     /locus_tag="WIB30_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ3796243.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14727..16061)
                     /gene="glmM"
                     /locus_tag="WIB30_000095"
     CDS             complement(14727..16061)
                     /gene="glmM"
                     /locus_tag="WIB30_000095"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ3796244.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16191..17042)
                     /gene="folP"
                     /locus_tag="WIB30_000096"
     CDS             complement(16191..17042)
                     /gene="folP"
                     /locus_tag="WIB30_000096"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ3796245.1"
                     /translation="MARHGWQAGRFEIGLDKPKIMGIVNLTPDSLSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSPEEEWARVAPVLAEVAGWGVPVSLDTRH
                     TAVMEKALALGGIDIINDVAALTDEGALELLARQADTGICLMHMQGLPKNMQINPKYQ
                     DVVGEVTRYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGL
                     PLLIGVSRKSMIGELTGETDAAARVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGINL"
     gene            complement(17182..18075)
                     /locus_tag="WIB30_000097"
     CDS             complement(17182..18075)
                     /locus_tag="WIB30_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ3796246.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGNLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18166..20280
                     /locus_tag="WIB30_000098"
     CDS             18166..20280
                     /locus_tag="WIB30_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ3796247.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGTPAIRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALR
                     NNSIKIETVNGAFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20344..21822)
                     /gene="ubiD"
                     /locus_tag="WIB30_000099"
     CDS             complement(20344..21822)
                     /gene="ubiD"
                     /locus_tag="WIB30_000099"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ3796248.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWGELGL"
     gene            complement(21842..22357)
                     /locus_tag="WIB30_000100"
     CDS             complement(21842..22357)
                     /locus_tag="WIB30_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ3796249.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22364..22843)
                     /locus_tag="WIB30_000101"
     CDS             complement(22364..22843)
                     /locus_tag="WIB30_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796250.1"
                     /translation="MYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAALFITLFFA
                     AVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRR
                     VTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            complement(23078..23323)
                     /locus_tag="WIB30_000102"
     CDS             complement(23078..23323)
                     /locus_tag="WIB30_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ3796251.1"
                     /translation="MTEQEIYRLLRDTLTELFEIEPERITPDTNLYEDLEIDSIDAID
                     LIDRIKRETGRKLQAEDFRNVRTVNDVVQAVLKIQAG"
     gene            complement(23335..23595)
                     /locus_tag="WIB30_000103"
     CDS             complement(23335..23595)
                     /locus_tag="WIB30_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ3796252.1"
                     /translation="MNDLENQIKQLIIDSLALEDITAADIGSEDALFGDGGLGLDSVD
                     ALELGLAVQKHFGFRLDGEQENLREHFANVKTLAAFVKSRQA"
     gene            complement(23592..24350)
                     /locus_tag="WIB30_000104"
     CDS             complement(23592..24350)
                     /locus_tag="WIB30_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ3796253.1"
                     /translation="MDKLDYCRRFFATWLGFVIFGVGGIMMKLVLLPYTLNGTSGSVA
                     RQLAARRIIGTSWRLFVAYLKWSGVLEVSFKGVEKLNRPGQLILANHPSLLDVVLLVG
                     HVPKMNCIVKKDLQHNPAMSSQIKGAGYIPNEESEAMLETVKAVFDSGQSLLVFPEGT
                     RTGWDGRVKMHRGAVSLGLRYAEVITPVCIKMNPPNFKKGQPWYRIPPKRIRYEITVG
                     DDILPQDWLAEKPLPIAARRLNEYLQDYFTRKTI"
     gene            complement(24343..25098)
                     /locus_tag="WIB30_000105"
     CDS             complement(24343..25098)
                     /locus_tag="WIB30_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEZ3796254.1"
                     /translation="MPSIICRFHFDIAAWRVSGSKMRDMAQWAKWAECPDFGDGLPDV
                     RPELPFLPAMQRRRLSKAARLVCDAAWDIASAHPGSPVVYASHDGEMARSFDLWLELL
                     KSHTVSPTSFGLSVHNATAGQWSILRQDMSEQTALAVCADGVETALAEAASLLEEGCG
                     SVLVLAADDPLPEGYAVSATRAPFAYALAMVLTKGTRYSLTLSASDDMPSEAGMLPEA
                     YWSGLEWVRFLLNGSRECRRVYRNREWLWQRNG"
     gene            25253..25786
                     /locus_tag="WIB30_000106"
     CDS             25253..25786
                     /locus_tag="WIB30_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
                     /protein_id="HEZ3796255.1"
                     /translation="MPVFASLPPQYMDTLLECPITNTASLLPHSGRMVLIDRITRYGD
                     DFVEAGAQVSPNHILLLDDKLPYTAFIELMAQAVGAYAGIQARKNARSVRLGFLLGTR
                     KLEIFAQSVPIGTHLLATAHMSIQDAGGMGVFDCELRWTDAPETSSETLPSDGILARA
                     SLNVYSPEHPAGTTDAV"
     gene            25814..26542
                     /gene="fabG"
                     /locus_tag="WIB30_000107"
     CDS             25814..26542
                     /gene="fabG"
                     /locus_tag="WIB30_000107"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ3796256.1"
                     /translation="MTETVLITGSNRGIGKAVAFGLAEDGFDIAVHCRSRRDEAEAVA
                     EEIRALGRNARVLQFDVSDREACREILTADIEANGAYYGVVLNAGLTRDNTFPAFSDD
                     DWDVVLRTNLDGFYNVLHPLVMPMIRRRKAGRIVCMASVSGLTGNRGQVNYSASKAGI
                     IGAAKALAVELAKRKITVNCVAPGLIDTDIIDENVPVEEILKAVPAALMGLPEEVAHA
                     VRFLMDEKAAYITRQVIAVNGGLC"
     gene            26536..27786
                     /locus_tag="WIB30_000108"
     CDS             26536..27786
                     /locus_tag="WIB30_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ3796257.1"
                     /translation="MLNTRRVAVTGIGGITAFGRDWQSIQAAFKAEKNAVKYMDWHER
                     FPELEAQLGAPIENYAPPKHWTRKQLRSMGRVSYLCVDAAEQALADAGLLGDESITDG
                     RMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTYVRMMPHTTAANIGIFFGLKGRI
                     IPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFPSEVYVFDSLYAASRRNGEP
                     EKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGANSDAYHISTPR
                     PDAQGAILAFQTALQHANLAPEDIGWINLHGTGTHHNDNMESRAVAAVFGNNTPCTST
                     KPQTGHTLGAADAIEAAFAWGIADRQSNPEGKLPPRLWDGQNDPNLPAINLTGSGSRW
                     ETEKRITASSSFAFGGSNCVLIIG"
     gene            27840..27915
                     /locus_tag="WIB30_000109"
     tRNA            27840..27915
                     /locus_tag="WIB30_000109"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27873..27875,aa:Ile,seq:cat)
     gene            27990..28748
                     /locus_tag="WIB30_000110"
     CDS             27990..28748
                     /locus_tag="WIB30_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ3796258.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQPPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            28749..29813
                     /locus_tag="WIB30_000111"
     CDS             28749..29813
                     /locus_tag="WIB30_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ3796259.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRDCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
     gene            30200..31537
                     /locus_tag="WIB30_000112"
     CDS             30200..31537
                     /locus_tag="WIB30_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ3796260.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
BASE COUNT         7245 a         8932 c         8182 g         7259 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctataaattc ccataaaaaa acggagcaga tacctgcccc
       61 gtttttattt aatccgaaat tttaatctaa atttagaatt ttgcaccgga ttggtttgcc
      121 atatagtcaa cagccgcttt gacttcgtca tcgctcaaac ctgcattgcc gcctttggca
      181 ggcatcgcgt taaagccttc aagggcgtgt ttgtgcaagg tttctttgcc ttttttgata
      241 cgcggtgccc aatcgtcttt tttgcctatg ccgggaatac cgggaatcga accgccgtgg
      301 cacacctgac aggttgcttc gaagactttt ttaccgtcaa cgccgaccgc aggggctgcc
      361 gcacccttgt cttctgcctt cgcttctgcc ggagctgcac tatcggcagg agcagaagct
      421 gttcctgaag cggcattgtc ggcaggcgca gcctcatcag gattcgggaa agaaccgccg
      481 cttttgttcg ccatgtaagt aatcgcccgt ttgagttcct gatcggtcag gtctgccgca
      541 ccgcctttgg caggcatggc gttaaagccg ttcagcgcgt gttggaacaa ggtatcgaag
      601 ccttgcgcga tacgcggtgc ccaatcgccg ttgtgttcca gtttcggagc gttcggcaca
      661 ttgctgtccg ccgcgtggca ttggatacag attttgccga aaatctgttc gccttggcgt
      721 tcgccgacgg ggatgccgtc gcccatcgtc aattgtccga caggctggat acgggtctgc
      781 gttgctgctt ccgtagtggc atcgacatcg ccgaacgagc cgctgcccgc cagcttaatc
      841 aggaaataaa ggactgcaat aacaataacg ataccgctca caagggtaaa cagtgcagag
      901 ccttgggctt tattgtcgcg gagttgtttc atttggtagg cctcgccgtc aggttaggtt
      961 gtgctgtaaa ttatagtttg gtgtgttaaa cgcagttaac aatattttgc tggattatac
     1021 tgaattcaca gggtctttcc aatcgctatc attgaaaata tgaaaaaatt tgccaacggt
     1081 atctgtataa aacaaacaat cctttgaaaa taattgttta tcctcaagaa aactctcctt
     1141 atgccgccat acgccgcctg ccggcgcaag ataacctttg ccaatttgca gaatttacgt
     1201 taaccttacg ttttacgcac ccatagctca gttggaagag tgtcagtttc cgaagctgga
     1261 ggtcacaggt tcgatccctg ttgggtgcgc caattataaa gagaccgtct gaaagataaa
     1321 tatttttcag acggtctttt gacttacttc aaactcttat ttcaagactt ccgcaaatgc
     1381 gcgggcaaca tagtcggtat tcgacgtatt cagtccggcg acgcacatcc tgccggaatc
     1441 cagcaggtaa acggcaaatt cgtcgcgcag cctgcggact tgttccacgc tcaatcctgt
     1501 gtagccgaac atgccgcgct gtttgatgaa ataagtgaaa tcgcgatcgg ggatttgcgc
     1561 agttaagaca tcataaagtt tctgccgcat cgcacggatg cggtcgcgca tcatataaac
     1621 ctcgttttgc cacaaggcgt aaagttcggg gctgttcatc acgtcggcgg cgatatacgc
     1681 gccgtgcgcg ggcgggctgg agtagatgcg gcggacggtg aatttgagtt gtccgaacac
     1741 caaatccgct tcttccttat tcgggcaaac cacgctcagg ccgccgacgc gctcgccgta
     1801 gagcgacagg ttttttgaga aggaattgct gacgaacaag ggcaattcca tttccaccgc
     1861 tttgcggacg gcgtaggcat cgctgtccaa atcgccgccg aagccttggt aggcaatgtc
     1921 cataaacgga atcagtttgc gcgttttgat gatgtgcaac acttcgtccc attgttgttc
     1981 cgacatatcc acgccggtcg ggttgtggca gcagggatgg aggatcagga cgctgttttc
     2041 aggcagggtg ttgaaaaacg cggtcatttc gtcgaatttc acgccgacag tggcagggtc
     2101 gtaatatggg taagtgccga cctcgaaacc tgcgccttca aaaatgccgc gatggttgtc
     2161 ccaagtcggg tcgctgacgt aggcgcgcgc ttcgggaaac cagcggtgca ggaagtccgc
     2221 cccgactttg agtgcgcccg agccgcccaa ggtttgcacg gtaacgatgc gtccttgcac
     2281 aagcgcgcgg ttgtctttgc cgaacaataa atgctgtacc gcgctgcggt aagtgtccaa
     2341 gccttccatc ggcaggtagg gcgacggcgc aggcgcggcg gcacgggcgg tttcggcgcg
     2401 gctcacggat tccaacacgg gcattttgcc ttcgtcgtcg aaataaatgc ctatgctcaa
     2461 attgactttt tcggggcgcg ggtcgttttt gaaggtttcg accaaactca aaatcgggtc
     2521 gccgggatag tattcgatgt gtcggtacat agtccttacc ttttactttt tcaaaggatt
     2581 ttctttttca acaatacacc actttcgata tggtgcgtaa acgggaattg gtcgaacagg
     2641 gcggcacgtt cgaccgcatg ggtttccgcc aaggtgtcca aattggcacg cagcgtttcg
     2701 ggattgcagg aaatgtagat gatgttgtca aactgcgaca ccagcttcaa agtttcctca
     2761 tcgataccgg cacgcggcgg atcgacgaaa atagtggaaa atgcgtaatc cgtcaaagca
     2821 ataccgccat ccttaaggcg tttaaactca cgttttccgg tataggcttc agtaaattct
     2881 tcagcagaca gacgggcgat tttgatgttg ccgatgcggt tggcttcgat attccattgc
     2941 gccgcgctga cggaggtttt ggagatttcg gttgccaaaa cacgttcaaa cttttcagat
     3001 aaaggaagcg taaagttgcc gtttccgcaa tacagttcga gcaaatcccc atccaatcct
     3061 tctgctgcac cgcaggccca ttcaagcatt ttctgacaca cggcggcatt cggttgggta
     3121 aaactgcctt caatttgccg ataacggaaa tcccggttgc cgaccttcaa agtttccgtt
     3181 acatagtcct gttttaagac tattttctgt cccctgctcc gcccgatgac ggaaatatcc
     3241 aactgttgct gtaacgcttg cgccgcctgc atccactcat tatcaagcct tttgtggtaa
     3301 atcatggtaa ccagcatttc cccgctgagc gtagacaaaa attcgacggc ataccaacgg
     3361 tttctcagtt cggcagattg ggcggcaacg gcaatcaatt ctggcatcag gcggttgacg
     3421 gcttcggaag ccgcttcaaa acggtcgcaa cggatcaggc ttgccccgcc agccttttgt
     3481 cctttttcaa acatggcata aaacatttca ccgccttcgt gccaaatacg gaactcggca
     3541 cgcatacggt aatgtttgtc cggagattcg tacacttccc actcaggaac atccaaacct
     3601 gcaaaaaggg ttttaaggtg gtctttttta tcttgaagct gctgcgtgta gtcgctcata
     3661 tcgctatcct gaaaaatcag accggattat aagccgattc actgcaaaac acgcaacccc
     3721 tttaccggca tataacccgt tttcctccaa cttttatgct aaaatccgcc gtctccaaca
     3781 taccgccgac agaaggaaaa tatggcaggc aacactttcg gacaactctt caccgtgacc
     3841 acgttcggcg aaagccacgg cgcaggtttg ggctgcatca tcgacggctg cccgcccgga
     3901 ctggcattaa ccgaatcaga catccaagcc gacctcgacc ggcgcaaacc cggcaccagc
     3961 cgccacgtta cccaacgccg cgaagccgac caaatcgaaa tcctctccgg cgtattcgaa
     4021 ggcaaaacca ccggcacgcc catcgccctc ttaatccgca ataccgacca gcgcagcaaa
     4081 gactacggca acatcgccca aagtttccgc cccggccatg ccgactatac ctattggcac
     4141 aaatacggca cgcgcgacta ccgcggcggc ggcaggagct ccgcccgtga aaccgccgcc
     4201 cgcgttgctg ccggagccgt tgccaaaaaa tggttgaaag aaaaattcgg cacggaaatc
     4261 accgcctacg ttacccaagt cggcgaaaaa gaaatccggt ttgaaggctg cgaacacatt
     4321 tcccaaaatc ctttttttgc cgccaaccat agccaaattg ccgagctgga aaactatatg
     4381 gacagcgtgc gcaaatcctt ggattccgtc ggcgcgaagc tgcatattga agccgccaac
     4441 gtccccgtcg gcttgggcga acccgtattc gaccgcctcg atgccgaaat cgcctacgcc
     4501 atgatgggca tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg
     4561 caacgcggca gcgagcatgg cgacgagctc acgcctcaag gcttcctatc caaccactca
     4621 ggcggcatct taggcggcat cagcaccggg caagacatct gcgtcaatat cgccatcaaa
     4681 cccaccagct ccatcgccac cccgcgccgc agtatcgaca tcaacggcaa ccccatcgaa
     4741 ctcgccacgc acggcagaca cgatccctgc gtcggactgc gcgccgcgcc gattgcagaa
     4801 gccatgctcg cgttagtcct catcgaccac gccctgcgcc atcgcgcgca aaatgccgac
     4861 gttcgggtta atacgcccga cattaccctt tcaaacaaat aaaaatttag ccaaaacaca
     4921 gactttataa tagaataccg agcattgccg tgccgctttg acgcacgggt ttgtttagag
     4981 gaatacaacc gaaatgacac aagaaaccgc tttgggcgcg gcactgaaat ccgccgtcca
     5041 aactatgagc aaaaagaaac agaccgaaat gatcgccgac catatctacg gcaaatacga
     5101 tgtattcaaa cgcttcaaac cgttggcgct cggcatcgat caggatttga ttgccgcttt
     5161 gccgcaatac gattccgcac tgattgcacg cgtcctcgcc aaccactgcc gacgtccgcg
     5221 ctatctgaaa gccttggcgc gcggcggcaa acgtttcgat ttgaacaacc gtttcaaagg
     5281 cgaagttacc cccgaagaac aggcgatcgc gcaaaaccat cctttcgtgc agcaggcttt
     5341 gcaacagcag tccgcccaag ctgccgccga aacgccgtct gttgaagccg aagcagccga
     5401 atcttccgca gcagaataaa tccccaaatg aaatgccgtc tgaaaaccga tttggtttca
     5461 gacggcattt ttccgtatgc ggcaatcacg gttcaaatat cgagttccgc cgtatcgcct
     5521 tcgcgttcca tccaagcgcg gcgggcggcg gcttcgcctt tgcccatcag tttgacgaag
     5581 atgtcgcgcg tctcgtcatc tgcaccttct gggatttgta cctgcaacag gcggcgggtg
     5641 tcagggtgca tggtggtgtc tttgagctgg tcggggttca tttcgcccaa gcccttgaaa
     5701 cggctgatgg aataggcggt ttctttgacg ccttcttttt gcagccgctc caaaatgctg
     5761 tcgagttcgt tttggtcgag ggcgtagaat ttgcgggcag gtttgctttt gccttgtgcg
     5821 ttgacatcga cgcggaacag cggcggctgg gcgacgtaga tgtgtccgtc ggcaaccagt
     5881 ttcgggaagt ggcggtagaa taaggtcagc agcaaaactt gaatatgcga gccgtccacg
     5941 tcggcatcgg acaggatggc gattttgccg tagcgcaggc cgcttaaatc gggatggtcg
     6001 ttaataccgt gcggatcgac gccgatggcg acggaaatgt cgtggatttc ggcgttgccg
     6061 aagagttggt cggggtggac ttcaaagctg ttgagcactt tgccgcgcag gggcaggatg
     6121 gcttgggtgg ctttgtcgcg ggcgagtttg gctgagccgc cggcggaatc gccttcgacg
     6181 aggaagagtt cgttttcgcg gatgtcttcg ctttcgcagt cggtcagctt accgggcagg
     6241 acggcgacgc cgctgccttt tttcttttca atctttttaa ccgaacgcat ccgcgcctgt
     6301 gcctgacgga tggcgagttc ggcgattttt ttgccgaagt ccacgttttg gttcagccac
     6361 agttccaaag ggtcgcccga tacggtagca acgagtttca gcgcgtcgcg gttggtcagt
     6421 ttgtctttgg tctgaccttg gaactgcggg tcgaggacac gggcagagag gacaaaggca
     6481 gttttgccga acacgtcgtc gctttgcact ttcacgccgc gcggtaagag gttgtgcagg
     6541 ttgatgaagt tgctgacggc gttgaacacg gcttgtttca agcccgcttc gtgcgtgccg
     6601 cccagcgggg tggggatgag gttgacatag ctttcgttgg cacacgagcc ttcttccagc
     6661 caagtcaggg caaatgctgc gccttcgccg atgctgaaat cgccgttgtg ttcgtctgaa
     6721 atgtagtttt cgcaagagaa cagcggtacg gcttcctgcg cgtcggcaat caggtcggtc
     6781 agatagcttt tcaggccgtc ggggtaatgc caggtttggg tgtgcgcttc gtcttcgcct
     6841 ttgaccggac gggtcaggga aacgcgcaca cccggcagca gcacggcttt ggcacgcagc
     6901 aggcgttcga gttcgggaat gctgtaattc gggctttcaa aatatttgcc gtccggccac
     6961 acgcgcactt cggtgccgct gtctttgacg gcgcatttgc ccacttgtgc caacggttcg
     7021 accacgtcgc cgccggcaaa cacgatacgg tggattttgc cttcgcgttt gaccgttacc
     7081 tcaaggcggg tggaaagggc gttggtcacg gatacgccca cgccgtgcag gccgcctgaa
     7141 aaggcatacg cgctgccgcc gtcttttttg ttgaacttgc cgcctgcgtg cagacgggtg
     7201 aacacgagtt cgactacggg tacgccttct tcagggtgca gaccgacggg aataccgcgg
     7261 ccgttgtcgc gtacggaaag cgaaccgtct tcatgaattt gcacgtcgat tgccgtagcg
     7321 aaaccgccca acgcctcgtc cgccgcgttg tcgatgactt cttggcagat atgggtcggg
     7381 ctgtcggtgc gggtgtacat accgggacgt tctttgaccg gttccaaacc tttgaggacg
     7441 gtgatgctgg attcgctgta ttggttgttt ttagccatag gaataatctg aaagtaagaa
     7501 aaacaacgct ttcagacggc ctgaaagcgt tgcgttccgt tgttttaccg gttgccggaa
     7561 agttggcggg cggcaaagtc ttcataactt tccataccgc gcaggaagcg ggaagaaagt
     7621 tctttcaatg cccccaaata aacgtcgcgt ttgaaatcaa tcacttcgtc aacgggcgcc
     7681 caatattggt gccaacgcca gccgtcaaat tcggggtggc gggtggcgcg caggttgaca
     7741 tcgcaatccc ggccggtcag gcgcaggaga taccaaatct gcttctgtcc gcgataagag
     7801 ccgcgccatt cgcggcgtac ccagttgttc ggcacgtcgt aacgcagcca gtcgcgcgtc
     7861 cgcccgacga ttttgacgtg ttgcggcaga agtccgactt cttcgtaaag ctcgcggtac
     7921 atcgcggttt cggggctttc gcccggcttg atgccgcctt gcggaaactg ccaagaatgt
     7981 tcgcgcacgc gcttgcccca aaagacttcg ttacgttcgt taatcaggat aataccgaca
     8041 ttggggcgat agccttccct gtccaacacg gtgtcgccct ccgttaaatt caatcgtacg
     8101 attttcccac aaatcgggcg gttttgacaa atcagacggc atggttcaag catgtcgaaa
     8161 cacagacgga ctcgggaaaa tatcttaaat ttggtttaca ataatgtgtt tcaaattatt
     8221 cgggaatcag accatgttag atatccaatt gctccgcagc aacaccgccg ctgtggcgga
     8281 acgcttggcg gcgcgcggtt acgagtttga tgccgcgcgt ttcaatgcgc tggaagaaca
     8341 acgcaaagcc gtccaagtga aaaccgaaga actacaagcc tcgcgtaaca gcatttctaa
     8401 acaaatcggc gcactgaaag gtcagggcaa acacgaagag gcgcaggcgg ccatggatca
     8461 agtcgcccaa atcaaaaccg atttggaaca ggctgccgcc gatttggatg ccgttcaaaa
     8521 agaattggac gcatggttgt tgagcatacc taacctgccg cacgaaagcg tacctgccgg
     8581 taaagacgaa accgaaaacg tcgaagtccg caaagtcggc accccgcgcg aatttgactt
     8641 tgaaatcaaa gaccatgtcg atttgggcga acctttgggt ttggattttg aaggcggtgc
     8701 aaaactctcc ggcgcacgat ttaccgtgat gcgcggacaa atcgcccgtc tgcaccgcgc
     8761 cttggcacag ttcatgctgg atacgcacac gctgcaacac ggctacaccg agcattacac
     8821 gccttatatc gttgacgata cgacgctgca aggtacgggc caactaccaa aatttgcgga
     8881 agatctgttc cacgttaccc gtggcggcga cgaaaccaaa accacccaat acctgattcc
     8941 gacagccgaa gttaccctga ccaataccgt tgccgacagc attatcccgt ccgaacaact
     9001 gccgctgaag ctgaccgcgc attcgccctg tttccgcagc gaggcgggtt cgtacggcaa
     9061 agacacgcgc ggtctgattc gccagcacca gttcgacaaa gtggaaatgg ttcaaatcgt
     9121 tcatcccgaa aaatcatacg aaacgctgga agaaatggtc ggccatgccg aaaacatcct
     9181 gaaggctttg gaactgccct accgcgtgat taccctgtgt accggcgaca tgggcttcgg
     9241 cgcggcaaaa acgtatgact tggaagtttg ggtgccggcg caaaatacct accgcgaaat
     9301 ctcaagctgc tccaactgcg aagatttcca agcccgccgc ctgaaggcgc gtttcaaaga
     9361 cgaaaacggc aaaaaccgct tggtacatac tttgaacggc tccggtttgg cagtcggcag
     9421 aaccttggtc gccgtgttgg aaaaccatca aaacgccgac ggcagcatca acatccctgc
     9481 cgcactgcaa ccgtatatgg gcggcgttgc caagttggaa gtgaaataag ccttaaccct
     9541 gaacataaat gccgtctgaa acctggttca gacggcattt cctttaaact tttaaaacac
     9601 atcaaccgtc ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc tcgcggatat
     9661 tggacagcgt aacttccgaa atattaccca acgcctcttc cgtcaaaaat gcctgatggc
     9721 cggtaaacag cacattatga caagacgaca ggcggcggaa cacgtcgtcg gtaatcacat
     9781 cgttggattt gtcttcaaaa aacaattcgc gttcgttctc gtacacgtcc atacccaatg
     9841 cgccgatttt ccggcgtttc aacgcctcaa tcgcggcaga actgtcaatc agcccgccac
     9901 ggctggtgtt gataatcatc acgccgtctt tcattttgtc gaacgccgct tcgttcagca
     9961 tatagtggtt ttccggcgtg gaggggcaat gcagcgtgat gatgtccgac cgggcataca
    10021 gctcgttcaa ctccacatac ctgccgccga ttttttccgc ttcggggttg caaaacggat
    10081 cgtaagccag caggttcatg ccgaaaccct ttaaaatccg catggttgca ataccgattt
    10141 tccccgtgcc gataacgccc gccgttttgc cgtacatatt gaaaccggtc agaccttcca
    10201 gcgaaaaatt cgcatcgcgg gtacgctgat aggctttgtg gatacggcgg ttcaacgtca
    10261 gcatcagacc gaccgtatgt tccgcaaccg attcgggcga ataggcaggc acgcgcacga
    10321 ctttcaagcc caactcttca gccgccttta aatccacatt attgaagccg gcacaacgca
    10381 acgccacagt tttcacgcca atttgcgcca atttttccaa cacgggccgg ctgccgtcgt
    10441 cgtttacaaa aatacagacc gcttccgcgc cttccgccat tttcgccgtt ttcgcatcca
    10501 gcataaaatc aaaaaactcc agctcgaagc cgaaatgccg gttggcgcgg gtaaaatgtt
    10561 cgcggtcata gcttttcgta ccgtaaatcg caatcttcat caatatgtcc agttgtcgcc
    10621 tatggttgag aaacggcata atacactgaa ttcaaacaaa tcagtagaat atggcagatt
    10681 aaaattgatt gcatgcacgg catttccatt tcaaaacaca aaactcaatc gcccattgcc
    10741 gccagaagct ccgcctgatg ctcggcaatc agggcattgg tgatttctgc caagtcgccg
    10801 tccatcacaa aatccagctt gtgcagggta aggttgatgc ggtggtctgt taccctgcct
    10861 tggggatagt tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat gagggatttg
    10921 cgttcggcgg cttctttggc ttgggcttcg cgtttttgcg cgtcgttcag gcgggcggcg
    10981 aggactttca tcgcctgcgc tttgttggcg tgttggctgc gcccgtcttg gcactcgacc
    11041 accatgccgg tgggcaggtg ggtgatgcgg acggcggagt cggttttgtt gatgtgctga
    11101 ccgcccgcgc cggatgcgcg gaaggtgtcg atgcgcaggt cggctgggtt cagttcgatg
    11161 tcttccagtt cgtccgcttc gggcatgacg gcaacggtgc aggcggaggt gtggatacgg
    11221 ccttggcttt cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc aaatttcaaa
    11281 cggctgtacg cgcctaagcc gataatacgg gcgataactt ctttatagcc gcccaactcg
    11341 ctttcgttgg cggacacgat ttcaacctgc cagcggttgc gttcggcgta gcggctgtac
    11401 atacgcagca aatcgccagc aaacagcgcg gcttcgtcgc cgcccgttcc ggcgcgtatt
    11461 tcgatgaaga tgtttttgtc gtcgtcggca tctttgggca gcagcagttt ttgcagttcg
    11521 gtatcgagtt cgccgatttt ggctttcgcc gcttcgattt cttcggcggc aaagtctttc
    11581 atttcggggt cggacaacat ttcttcggca tccgccaagt cgctttgggc aagccgatag
    11641 ttttggaaca cttcgacgac gggggtcagt tcagcgtgtt cgcgcgtgag cttgcggtag
    11701 ttgtccatat cggacgtggc ttcgggttgc cctaaaaggt gggtaacttc ttccaaccgg
    11761 tcgccgagtt gttgcagttt ttctaagata gacggcttca taattcttcc ataacaaacg
    11821 ccgcctgaat ttcagacggc atcaacactg aattattata ataggttttc cggacattca
    11881 aaaagataat ctcagatgga taacctaccg tcccaacagg gcatcggcat tgcgctccgt
    11941 taccttggca atctcttcca cacaggttcc gcggatttcc gcagcaatct ttgcaatacc
    12001 cggaatattg gcaggcgtat taatctcttt tttcagcata aacgggctgt cggtttccaa
    12061 tacgaaatcc ccgtcgttca aggctttaag cgtatcgcgc actttacgcg cgttcggatt
    12121 gagcaacaac gaaccgatgc cgattttgaa acccagtttc gtcaacacgc gcgcttcttc
    12181 cgcgctgcct gagaaggcgt gaacgatgcc gccttgggca aagcctgtct gtttgacggc
    12241 ggcggcgatg tcggcggtgg ctttcagatt atggataatc acgcggcggc gcagggtttg
    12301 cgcgatttca agctggcgga cgaaaacttg gatttgccgt tcgcgctgcg gcggcgtttg
    12361 ggttttgtcg taaaaatcca agccgatttc gccgacccat gctgtcggat gcgctttcaa
    12421 tgtttgttcc aaaagctgga aatccggttc ggaaacgtcg tctgaaaacc aaggatggat
    12481 gccgagcgcg atatggatat tgccccaaag cggattttca gacaaccttt gagccaaatc
    12541 cgccacgtcc tgccaatcct gcggccgagt cgcgggaacg ataaaccgcc ctacccctgc
    12601 ctcccgcgct gcggtcagga tgtgcggcag gttttcgcgc agggcggcat cggcaaggtg
    12661 gcagtgggtg tcggtgaagt tcatttcgat tccgcaatgc ggcaggttca cataaaaata
    12721 aggcagggcg gcaagggatg cagtgtacgg gcggcgcata aggcgcggtc aacgccccgt
    12781 cattgccatt tcaatccgct ataacttcag tcttgccaac tctttttcca cctcttcctt
    12841 accccagcct tgcgatacgg cgagggtcat aagcgcggtg gcggtttcga ggttgcattt
    12901 gccgccgttg atgatgcctg agttgcggaa cgcgttgcct tgcgcgtaaa cggcagcggt
    12961 tctgccttgt cggacttggc tgatgttgag cagcagtttg ccttgctgcg taaagtcttg
    13021 cacggcgcgg atgaaacctt cgtctgcggg cgtgttgccg tgtccgtagc tttgcaggat
    13081 aagggcttgg gcgggaagct gtccgagtcc gtcggcaagt tcttggacgg caaagccggg
    13141 gataagcgtg cggacggcga tttttgcctg cgggtcagga tagcggattt tgaggccgtc
    13201 tgaaacggcc gccgcatctt gggacggaat acgaagattg taccagcctt gagtttcatc
    13261 ccattcggcg atgacaccga aatgcggatt gtcgaaaccg gcggcggttt cggtgctgac
    13321 tttgctgctg ccgacggcgg gataaagttt gccgtcaaag gcaataagcg tctgcttcaa
    13381 tccgagtggg aatgcggcaa cggcagtcgc cagattgcgc ggcgcgtcgc tgcccacggc
    13441 gttgtacggc cattgggaac cggtcaggac gatcggcttg tccaaaccct gcagcgcgag
    13501 ggcgaacaga ttggcggtat aggcgagcgt gtccgtgccg tgcagcacca aaacgccgtc
    13561 gtactgcggc agcgcggcgg caatgatgtc cagccaagcg caccagtttt gcagttggac
    13621 ggcggaagaa tcaatcagcg tcccacaaac gtgccaatcg aaatccatgc cgtttgaaaa
    13681 cggcgcgagt gcctgcccga ccagcgcggt atcggggcgc aggccttcgc tgctttgcgt
    13741 catgccgatg gtgccgccgg tataaaggac aaaaattttc tgtttcatgg ctcatcctga
    13801 aaatgcaaag accgcctgaa acgcgaaatg cgtccggacg gtcgaaaatc tgatttactg
    13861 ctgcttctgt gccgcttttt tcgctgcctt ttccgccttg cgtttggcgc gtttttcgct
    13921 caattgtgcg cggtaaagct gataatgtcg gcgtttgcgc caccagaacc acgccagcgc
    13981 cgccgccagc acgcccaatg cgatgaagat gccggattgc aggctgtgca ttttcgccat
    14041 cagccaatcg atgttgtgcg cgccgtactc gcccaagtaa atccaaacgg gcacggaaat
    14101 cagcgcggca agcccgtcca taatcagaaa gcgcagatac gatactttgc ggctgatgcc
    14161 ggcggtaacg aaaacggcag tccgcaaacc gggcaggaaa cgagcgacaa ataacaccca
    14221 gttgccgtat ttgtcgaatt tttcctgaac ctgtgcgtaa cgtttcggcg tcatgatgcg
    14281 cgcaatcggt ttgaacttga ggattttctg cccccagatg cgtccggcgg cgaacatgat
    14341 gccgtccccg accaatacgc cgagcatacc gactgcaaac ataatatgcg gattggtata
    14401 acccataccc gaaatcacgc cgcctgttac caaggtcaaa tcctcgggga tcggcacgcc
    14461 gaaaccgcag atgaccaaaa cgaaaaacac ggccgcatag ccgtattcga caaaaaaggc
    14521 ttccaaaagg gcaaacatag cggatattcc attgtcggag ataaaaagtc agaacaagcc
    14581 gaaacatttt ctgcgcgaaa cggcattcta tcaaagatta tgccgtctga aagcggcaag
    14641 gggcagattc gcgccctctg ccggacgttc agacggcatt taaacaggaa aaccgccgct
    14701 gttgaaacgg ttttgcctga ttttgcctaa aggctgccga tggcggaagc aatacgttcc
    14761 gcaccgtcgc gcgcccaatc cgcctgccgc gcctccacca tcacacgcac gacgggttcg
    14821 gttcccgaag cgcgcaacac gacacgccct ttgccttcga gttctttttc cacttccgcc
    14881 aacacgtctt ttgaggcttc ctgccatttc tgaccttttt ggatgcgcac gttaatcata
    14941 gtttgcggat acggctgcca atcggcgcaa acggtggcga ggtcttggtt cagcgtttgc
    15001 agtgccgcca aaacttgcag cgcggaaata atgccgtcgc cggtgttgtg tttgtccatg
    15061 cacaaaatat ggccgctggc ttcgccgccg atgagccagc cgcgttggtt cagctgttcc
    15121 aacacatagc ggtcgccgac tttggcgcgg cagaaatcca cgccctgctc tttcagggcg
    15181 atttccatcg ccatattggt catgaccgtg ccgaccacgc cgccgatgtt gataccttct
    15241 cgggcgcggg ctttagcaat gacgtaaatc aggctgtcgc cgtcgtaaac cttaccgttt
    15301 ttatcgacca tcatcaggcg gtctccgtcg ccgtctaagg cgatgccgta gtcggcttca
    15361 tgctgtaaaa cggctgcctg gagtgtcttg gtataagtcg caccgcattt ttcattgata
    15421 ttgtagccgt tgggttcgtc gccgatgctg acgacttgtg cgcccagttc gtgaaacacc
    15481 ttgggtgcga caccgtaccc cgcgccgttg gcagtatcga taaccaactt caaaccccga
    15541 aggtcggaat ggctgggaaa ggtagatttg caaaattcga tatagcggtc gtccgcacca
    15601 ctgatgcggc gtgcgcgacc gaggcgtgca gaaggctggg ttttcatttc ttcgtcgatt
    15661 ttggcttcga tttccaactc gatttcatcg ctcagtttca cgccgccctc ggcaaagaat
    15721 ttgatgccgt tgtcggaata ggcgttgtgc gacgcggaaa tcatcacgcc ggcggacagg
    15781 cgcaacgcgc gggtcagata agccacgccg ggcgtgggca gcggtccggt ctgtaccaca
    15841 ttcacacccg ccgccgtaaa accggccacc aaagcggctt ccagcatata gccggaaatg
    15901 cgcgtgtctt tgccgatgag gacggtcggt ttctggtcgg tgtcgtgctg caccaaaacc
    15961 tgccccgccg catagccgag tttcaatacg aaatcgggcg taatcggaaa ctgcccgact
    16021 tcgccgcgca cgccgtccgt gccgaaatat ttttttgcca tgtgttgctc cgagaatgtg
    16081 aaccgttgtc cgagattata cagtctgttt gtgccctgct gtctgcaccg ttgatgccgt
    16141 ctgaagccgc cccgtccttt tcagacggca tgaagtatgc gaaccgctgt ttacagattg
    16201 atgcccaacg cttcccacac cttcaacgca tccgccgtcg ccttcacatc atgcacccgc
    16261 acgatttgcg cgccgcgcgc cacggaagcc agcgccgccg ccacgctgcc gtgtacgcgt
    16321 gccgccgcgt cggtttcgcc ggtcagctcg cctatcatgc tcttgcgcga tacgccgata
    16381 agcagcggca aaccggtttc cgccatcaat tcgggcaaat gccgcatcag cgcgatattg
    16441 tgttgcaggg ttttgccgaa gccgaaaccc ggatcgagcg tgatgcgttg cggggcgatg
    16501 cctgccgcaa tacattccgc cgcccgcgct ttcaaatacc gcgtcacttc accgacaaca
    16561 tcctgatatt tcggattaat ctgcatgttt ttaggcaaac cctgcatgtg catcaggcaa
    16621 atgcccgtat ccgcctgacg cgccagcaat tcgagcgcgc cttcgtcggt caacgccgcc
    16681 acatcgttaa taatatcgat gccgccgagt gccaacgctt tttccataac cgccgtgtgg
    16741 cgcgtgtcca aactgacggg aacaccccac cccgccactt ccgccaaaac aggcgcaacc
    16801 cgcgcccatt cttcttcagg cgaaacataa tccgcacccg gccgcgtcga ttcgccgccg
    16861 atgtcgagaa tgtccgcacc ctctttcaaa agccgttcgg cgtgcgccaa agcggtttgg
    16921 gcgttttgcg aatacgcgcc gccgtcggac aaagaatcgg gcgtgagatt cacgatgccc
    16981 atgattttcg gcttgtccaa accgatttca aaccgtcctg cctgccaacc gtgtcgtgcc
    17041 atctgaactc ctcccaaaat aaaaaacaga ttatatgccg tctgaaaccg tcttgtgcgc
    17101 ttcagacggc accgctattc gggcggcaga cggcatgttg tccgaatatc tgctccgcct
    17161 ttgaatctgc cggtatgcct gctatccgcc cgacttttca aaacaggttc cgacgattcc
    17221 gcacgcgcct gccgcctttg ccaagccgta caggatttcc tgcggcatat cgcggttcca
    17281 taatcccgta atattcgcaa tcacgggcag atggctgatt tggcggactt tcacgatgga
    17341 ttcgacatcc aaacggtagg gatggtcttt ggtatggctc aacacgcccg actcgcccag
    17401 aatcaggtgg cggttgccgc gcgagacgac atattctgcg gcattcaacc aatcttcggc
    17461 agaatggtgc ttgtctttac acagcaccaa gggaatgttc aggtttccgg cttcattcaa
    17521 tacggcaaga tcggacatca gcccgccgcc caaatacagg atgtccgccc ccgcattcaa
    17581 agccgcttcg acatggcgga cgttgcggac gcgcaccaat acgggtttcc ctgcgccatg
    17641 cgccgatgcg gtctgttccg ccaaccgtct gcaccgtccc cgcccttcat ccgcacttga
    17701 agtgtcgtat aagtttgccg aagtgaaaaa cggatctaga aacactgcat ccgcattgcg
    17761 ccatactgac ggttctgcgg cgatacggac ggtttccccg ccgccgaaag ccacgccttt
    17821 ggcggcaacg cggctgtctt ccgcccgatt ttcccgactg acggttttcc atgtatccaa
    17881 aatgcggacg gctttctcga cctccggcag cgtctgcacc tccctgacgc tcaaaaccct
    17941 atcgtcgccg attgcgccga tgacggtacg ctcgtcgccg tgagaaatgt gttctcgcag
    18001 acctctgctg cggataaagg cgacaacgcc ggcaatgtcc gcttcggcgg cacgcctgct
    18061 catgacaata atcatatttc ctcctgacac aagaaacggc ctgcccaaaa taggattttt
    18121 gcaagccgtg ttatactgtg gcgtgtttta cagattgttc gggctatgga tttattatcg
    18181 gttttccaca aataccgtct gaaatatgcg gtggccgtgc tgacgatact gcttttggcg
    18241 gcagtcgggc tgcacgcttc cgtatatcgc accttcacgc ctgaaaacat ccgcagccgc
    18301 ctccaacaaa gcattgcaca cacacaccgg aaaatctcgt ttgatgcgga cattcagcgc
    18361 aggctcctgc cccggccgac cgtcatcctg aaaaacctga ccattaccga acccggcggc
    18421 gaccagactg ccgtttccgt ccaagaaacc aaaatcggat tgagctggaa aaacctgtgg
    18481 tcggatcaga tacagattga aaaatgggtg gtttcgagtg cggaacttgc cctgacgcgc
    18541 gacgggaaag gtgtttggaa catccaagac ctgatcgaca gccaaaaacg ccaagcctca
    18601 gtcaaccgca ttatcgtcga aaacagcacc gtccgcctca atttcctgca ggaacagctt
    18661 atcctgaagg aaatcaacct caacctgcaa tcccccgatt cgtcggggca gccgtttgaa
    18721 agttcgggca tactggtttg gggaaagctg tccgtcccgt ggaaaagcag ggggctgttc
    18781 ctttcagacg gcatcggcac gcccgaaatc tcaccgttcc attttgaagc ttccacttcg
    18841 ctggacggac acggcatcac catttccacc accggcacac ccgccatccg tttcaacgcc
    18901 ggcggagcgg atgccgccgg cctcggcctg cgtgcagaca cttccttccg caacctccac
    18961 ctgaccgcgc aaatccccgc gctggcactc aggaacaaca gcattaaaat tgaaaccgtc
    19021 aacggcgcat ttaccgccgg cggcgaatat gcccgatggg acggttcgtt caaactcgac
    19081 aaagccaacc tgcactccgg catcgccaac atcggcaacg ccgaaatctc cggcagcttc
    19141 aaaacaccgc gccaccagac caacttctcc ctcaattcgc cgctcgtatg gacggaaaac
    19201 aaagggctgg acgcgccgcg cctgtatgta tcgacccttc aggataccgt caaccgcctg
    19261 ccgcaacccc gtttcatcag ccggctcgac ggttcgctgt ccgtaccgaa tctgcaaaat
    19321 tggaatgccg aattaaacgg cacattcgac cgccaaaccg ttgccgcgaa attcagatac
    19381 acacatgaag acgcaccgca tctggaagcc gccgtcgcac tgcaaaaatt gaacctgacc
    19441 ccctatcttg acgacgtgcg gcaacaaaac ggcaaaatat ttcccgacac cctcgccaag
    19501 ctgtccggcg acatcgaggc gcacctgaaa atcggaaaag tccaacttcc cggcctgcaa
    19561 ctggacgata tggaaaccta cctccacgcc gacaaagacc atatcgcgct cagccgtttc
    19621 aagtcagggc tttacggcgg ccataccgaa ggcggcatca gcatcgccaa cacccgtccc
    19681 gccacttacc gcctgcaaca gaatgcaagc aacatccaaa tccaaccgct gctgcaagac
    19741 ctgttcggct tccacagctt cagcggcaac ggcgacgcgg tcatcgacct gaccgcgggc
    19801 ggcgaaaccc gaaaagagct tatccgctcg cttcagggca gcctgtcgct aaatatttcc
    19861 aacggcgcgt ggcacggtat cgacatggac aatatcctga aaaacggcat ttcgggcaaa
    19921 actgccgaca atgccgcacc cagcacaccc ttccaccgat tcacgctcaa cagcgaaatt
    19981 tcagacggca tcagccgcca catcgatacc gaactcttct ccgacagcct ctatgttacc
    20041 agcaacggct ataccaatct ggatacgcag gaattgtctg aagatgtcct tatccgcaac
    20101 gccgtccatc cgaaaaacaa accgattccc ctgaaaatca ccggtacggt ggacaagccg
    20161 tccattaccg tcgattacgg caggctgacc ggcggcatca attcgcgcaa agagaaacag
    20221 aaaatcctcg aagacaccct gctggaacaa tggcagtggc tcaaacctaa agaaccgtaa
    20281 acatcctgcg tacaaaaatg ccgtctgaaa cgcccccgcg cttcagacgg cagaccgtaa
    20341 aacctacaac cccaattccc cccaaatccc atcaatctta gccgtaaccg cagggtcttt
    20401 tttgatgacg cgtccccatt cgcggtcggt ttctcccggc catttgttgg tcgcatccaa
    20461 acccattttg ccgccgagtc cgctgacggg gctggcgaag tcgagataat cgatgggcgt
    20521 gttttccacc aacacggtat cgcgtactgg gtccatgcgc gtggttaccg cccagatgac
    20581 ttccttccag tcgcgcacgt ttacatcgtc atcgaccacg atgatgaatt tggtgtacat
    20641 aaattggcgc aggaacgacc agcagcccat catcacgcgc ttggcgtgtc cggcgtactg
    20701 ttttttcatg ctcaccaccg ccatgcggta ggagcagcct tcgggcggca ggtagaaatc
    20761 ggtgatttcg gggaactgct tttgtaaaag cggtacgaac acttcgttca acgccacgcc
    20821 caaaacagcg ggttcatcgg gcggtttgcc tgtgtaggtg gaatggtaaa tcgggttttc
    20881 gcgcatagtg atgcgttcga ccgtgaacac gggaaaatga tcctgctcgt tgtaatagcc
    20941 ggtgtggtcg ccgtacgggc cttccaacgc ggtttcgttc ggatggatga cgccttccaa
    21001 cacgatttct gcgcgggcag gcacttgcaa atcgttgccg atacatttca ccagctccgt
    21061 ccgcgaaccg cgcagcagtc cggcaaactg gtattcgctc aaagtatcgg gaacaggcgt
    21121 taccgcgccc aaaatggtgg cggggtcgca accgagcacg acagcgacgg gatacggcgt
    21181 atcgggattg agtttgcgga attcctgata atccaacgcg ccgccgcgat gcgacagcca
    21241 gcgcataatc agcttgtttt tgccgattag ttgttggcgg taaatgccga gattttggcg
    21301 ttttttgtgc ggcccgcgcg tgacggtcaa gccccatgtt accagcggcg caacgtcttc
    21361 cggccagcaa tgctgaatcg gaagtttata caaatcaacg ttttcgcctt cccacacgat
    21421 ttcctgacac ggcgcgtttt tcaccacgtt cggcgccatg ctccaaatgt ctttcagtag
    21481 cggcagtttg gaaaacgcat ctttgatgcc tttgggcggt tcgggttctt tcaaatacgc
    21541 cagcgtctgc ccgatttcac gcagcttgga cacgctgtcc gcgcccatgc ccatcgccac
    21601 acgttcgggc gtgccgaaca ggtttgccaa cacaggataa ccgtagcgcg taccgtcggg
    21661 cttaaccggg ttttcaaaca gcaacgccgg cccttcggcg cgcagcacgc ggtcggcgat
    21721 ttcggtcatt tccaaatgcg gggaaacggg gtgcgcgatg cgtttgagtt tgccctgctg
    21781 ctcgagcatg gcgatgaagt cgcgcaggtc tttgtatttc atattcatcc tttttgtcct
    21841 tttatcctga gcaatccgat tcggataccg cccctatcct tgcctgcgct tcggcatatt
    21901 ctatgccgtg ataaaagtcg cgtaccagcg gatgttcgct gccttgatgg agttgcaaca
    21961 aaggacgttg accatcgggt tgggtaacga cattgcaatg caaaccgaag gtgtcggttt
    22021 cataaggggg cagccggttg cagatcatgc cgaaataaac agcgttttcc ggattgtcgt
    22081 gaaagtggct ttgatagtcg ttaaaactct tttcgctgac ggacacccac acgccatatt
    22141 ccagcgtttc ctcatgcccg ataatcggaa tcggcagcac cgcgcggata aagcggtcgg
    22201 tttggtcggg atagcggatg atgcagaaat cagaatcgca ttctgcttga taagcaatgc
    22261 gttcttcttc actgagttga ttatagggat cgggggcggt aaagccgatt gcgggcatat
    22321 cttcatgttt ttcgccgcag gaagtgcaag tgtacataag gtttcagact ttcaaaacga
    22381 gtttgcggta aagccattcg ccggcaaaca gcatccccat caatacatag gcaatcacgc
    22441 ctgtataaac cgcccaccaa tcatatcgcc ccaaccgggc caacaaagcg gcaagcgtcc
    22501 cgttgattat aaaaaatacg caccaaacct gcgttacccg gcgggtatag cgcacggctt
    22561 tttcaggcag gtcgggctgt tgcagccgcg caagtttttc aatcaccgtc tgcccggcaa
    22621 acaggctgcc gccgaacacc gccaacatca tcagattgac gaggacggga taccaataca
    22681 tcgaatcatg ccgcccgaac accaatactg cggcaaaaaa cagtgtaata aacaaagccg
    22741 cataacgctg ttggggcagt ttggcagtca gggcgcgcag cagccacaac ccgcacatcg
    22801 ccgccgccag ccaaacaaac cagcccgcct ccctgccgta tatagtggat taacaaaaac
    22861 cagtacagcg ttgcctcgcc ttgccgtact gtctgcggct tcgtcgcctt gtcctgattt
    22921 ttgttaatcc actataccac aaagcgggat aggcaatgct taatacggtc agaaaaatat
    22981 gtccgaaaaa accgggtttc attttgaatc cgcacaaatg ttttcagacg gcatccgata
    23041 aaaacatgcc gtctgaaaaa taattagaaa tacccgatta gcccgcctga atcttcaata
    23101 ccgcctgtac cacgtcgttg acggtgcgga cattgcggaa atcttcggcc tgcagcttgc
    23161 ggccggtttc gcgcttgatg cggtcaatca ggtcgatggc atcgatgctg tcgatttcca
    23221 aatcttcgta aagattggta tcaggcgtaa tccgttccgg ttcgatttca aacaactcgg
    23281 tcagggtatc gcgcaacaac cggtagattt cttgttcggt catatgtttc atccttatgc
    23341 ttggcggctt ttgacaaagg cggcgagtgt tttgacattg gcaaaatgtt cgcgcaagtt
    23401 ctcctgctcg ccgtccaatc ggaaaccgaa atgtttttgc accgccaagc ccaattccag
    23461 cgcatcgacg gaatccagtc ccagccctcc gtcgccgaac agcgcgtctt cgctgccgat
    23521 gtcggcggcg gttatatcct ccaaagccaa actgtcgata atcagttgtt tgatttggtt
    23581 ttccaagtcg ttcatatggt tttccttgta aaatagtcct gcagatattc attcagccgc
    23641 cgcgccgcaa tcggcaacgg tttctcggcg agccagtctt gggggaggat gtcgtctccg
    23701 acggtaattt cataccgtat ccttttcggg gggatgcggt accacggctg ccccttttta
    23761 aaattgggcg gattcatttt gatacatacg ggcgtaatca cttcggcata ccgcagtccc
    23821 aaagaaaccg cgccccggtg catttttacc cgtccgtccc accccgtcct cgttccttcg
    23881 gggaacacca gcaggctctg cccgctgtca aaaaccgcct ttaccgtttc cagcattgct
    23941 tccgactctt cgttcggaat atagcccgca cctttaatct ggctgctcat tgccggattg
    24001 tgctgcaaat cttttttcac gatacagttc attttgggca catggccgac aagcagcacc
    24061 acatccagca aagacggatg gtttgccaaa atcaactgtc ccgggcggtt gagtttttca
    24121 acacccttga acgatacctc caacacgccc gaccatttca gataagcaac gaacaaacgc
    24181 caagatgttc cgataatccg gcgcgccgcc aattggcggg cgacagaccc tgaagtgccg
    24241 ttcaaagtat aaggcaacaa aaccaatttc atcataatgc cgccgacacc gaaaatcaca
    24301 aatcctaacc aagtcgcaaa aaaacggcgg caataatcca atttatccat tccgctgcca
    24361 cagccattca cgattgcgat atacccggcg gcattctcga ctgccgttca gcagaaagcg
    24421 cacccattcc aaaccgctcc aatatgcctc gggcagcata ccggcttcag acggcatatc
    24481 gtccgaagca gacaaagtca ggctgtaacg cgtccctttg gtcagaacca tcgccaaagc
    24541 ataagcaaac ggcgcgcgag tcgccgatac ggcatatcct tccggcagcg gatcgtccgc
    24601 cgccaaaacc aaaaccgacc cgcatccctc ttccaacagt gatgccgctt ccgccaatgc
    24661 cgtttccaca ccgtcggcac acacggccaa tgccgtctgc tcgctcatat cctgacgcaa
    24721 tatcgaccat tgccccgccg ttgcattgtg caccgacaaa ccgaacgaag tcggcgacac
    24781 ggtatgcgat ttcaacagtt ccaaccacaa atcgaaactg cgtgccattt ccccgtcgtg
    24841 cgaggcataa actaccggac tgccgggatg ggcggaggca atgtcccaag ccgcgtcgca
    24901 taccaaacgc gccgccttac tcaaacggcg gcgctgcata gcgggcagga acggcaattc
    24961 cggcctgaca tcgggcaaac cgtcgccaaa atccggacat tccgcccatt ttgcccactg
    25021 ggccatatcg cgcattttgc tgcccgaaac ccgccaggcg gcgatgtcga agtggaaccg
    25081 gcaaataatc gacggcatag tttctttcaa aaatttacac tgtgccgcat tctaaccaaa
    25141 gcctatcccc ctgacaatgc cgaaattcaa acgcatttct gccccctttc tccgacaacg
    25201 ccgcccctcg gaaaaccgcc agaattagcc tgaatttaca tttatcatta taatgcccgt
    25261 atttgccagc ctgccgccgc aatatatgga cacactgcta gaatgcccga ttaccaacac
    25321 cgcctccctg ctgccgcaca gcgggcgtat ggttctgata gaccgcatta cccgatacgg
    25381 cgatgatttt gtcgaagcag gggcacaggt aagccccaat cacatccttt tacttgacga
    25441 caaactgccc tacacggcat ttatcgaact gatggcacag gctgtcggcg cgtatgccgg
    25501 tatccaagcc cgaaaaaacg cacggtcggt ccggctcggc ttcctgctcg gcacgcgcaa
    25561 acttgaaatc ttcgcccaat ccgtcccaat cggcacgcat ctgctggcaa cggcgcatat
    25621 gtctattcag gatgccgggg gtatgggcgt gtttgactgc gaactgcgtt ggacagacgc
    25681 gccggaaact tcgtccgaaa cactcccttc agacggcatt ttggcgcgcg cctcactcaa
    25741 cgtgtacagc cccgaacacc ctgccggaac aaccgatgcc gtctgaacag gcacacacat
    25801 acggagaaca acgatgaccg aaactgtcct gattaccggc tccaacaggg gcataggcaa
    25861 agccgtcgca ttcggtttgg cggaagacgg ctttgatatc gctgtccact gccgcagtcg
    25921 ccgcgacgaa gccgaagccg tggcggaaga aatccgcgct ttgggcagaa atgcgcgcgt
    25981 gttgcagttt gacgtgtccg accgcgaagc ctgccgcgag attctgaccg ccgacatcga
    26041 agcaaacggc gcgtattacg gcgtggtgtt gaacgccgga ctgacgcgcg acaatacctt
    26101 ccccgcgttt tcagatgacg attgggatgt ggtgctgcgg actaatttgg acggttttta
    26161 caatgtattg catccgctgg ttatgccgat gatacgccgc cgcaaagccg gacggattgt
    26221 gtgtatggca tcagtgtccg gcctgacggg caaccgcggg caggtcaatt acagcgcgtc
    26281 aaaagcaggc attatcggcg cggcaaaagc cttggcggtc gaactggcga aacgcaaaat
    26341 caccgtcaac tgtgtcgcgc cgggtctcat cgataccgat attatcgatg agaacgtacc
    26401 tgtcgaagaa atcttaaagg ctgtccccgc agcgcttatg gggctgccgg aagaagtggc
    26461 gcacgcggtg cgtttcctga tggatgaaaa agcggcgtac atcacgcgcc aggtgattgc
    26521 ggtgaacgga ggtttgtgtt gaataccaga agggtcgcag taacaggcat aggcggcatt
    26581 accgccttcg gccgggattg gcaaagcata caggcagcat tcaaagccga aaaaaacgcc
    26641 gtcaaatata tggattggca cgaacgtttc cccgaattgg aagcgcaact gggtgcgccg
    26701 attgaaaatt acgcgccgcc gaaacattgg acgcgcaagc agctcagaag tatggggcgc
    26761 gtgtcgtacc tgtgcgtcga tgcggcggag caggcgctgg cggatgccgg tttgctcggg
    26821 gacgaaagca ttaccgacgg acggatgggc gttgcctgcg gctcttccag cggcagcacc
    26881 aaagacatcg gcgatgtggg cgaattgttg ctgaccggca cgtcgcgcaa cttcagcgcc
    26941 aacacctatg tgcgtatgat gccgcacacc accgccgcca atatcggcat ctttttcggg
    27001 ctgaaagggc gcatcatccc gacatcgagc gcgtgttcgt ccggcagcca aggcataggt
    27061 tatgcctacg aagccatcaa atacggtctg accgatatga tgctggcggg cggaggcgaa
    27121 gaatttttcc cgtccgaagt gtatgttttc gactcgcttt atgccgccag ccgccgcaac
    27181 ggcgaaccgg aaaaaacccc gcgcccatac gacgcgaacc gcgacgggct ggtcatcggc
    27241 gaaggcgcgg ggattttcgt gctggaagaa ttggaacacg ccaaacggcg cggtgcgata
    27301 atttacgccg aactcgtcgg ctacggagcc aacagcgatg cctaccatat ttccacgccc
    27361 cgccccgacg cgcaaggcgc aatccttgcc tttcagacgg cattgcaaca cgcaaacctt
    27421 gcacccgaag acatcggctg gattaatctg cacggcaccg ggacgcacca caacgacaat
    27481 atggaaagcc gcgccgttgc agcggttttc ggcaacaata cgccctgcac gtccaccaag
    27541 ccgcaaaccg gacacacgct gggcgcggcg gacgcaatcg aagccgcgtt cgcgtggggc
    27601 attgccgacc ggcaaagcaa tcccgaagga aaacttccgc cccggctttg ggacgggcag
    27661 aacgacccca acctgcccgc catcaacctg accggcagcg gcagccgctg ggaaaccgaa
    27721 aaacgcatta ccgccagctc gtcgtttgcc ttcggaggaa gcaactgcgt cttaatcatc
    27781 ggatgaaata agtttgtcaa tcccaccgct atgctataca atacgcgcct actcttgacg
    27841 ggtctgtagc tcaggggtta gagcagggga ctcataatcc cttggtcgtg ggttcgaacc
    27901 ccaccggacc caccaattcc caagcccgga cgtatgtttg ggcttttttg ccgccctgtg
    27961 aaaccaaaat gctttgagaa accttgataa tgaaaaaagt cagcgtattg attgttgcca
    28021 aaaacgaagc aaaccacatt caggaatgta ttgaaagttg ccgtttcgat aaagaagtca
    28081 tcgttatcga cgactacagc accgacaata ctgccgaaat tgccgagggt ttgggcgcaa
    28141 aagtcttcag acggcatttg aatggggatt tcggagcgca aaaaacattt gccatcgaac
    28201 aggcaggcgg agaatgggtt ttcctgattg atgcagacga acgctgcacg ccggaactat
    28261 ctgatgaaat ctcaaaaatt gtccaaaccg gcgattatgc cgcctatttt gtcgaacgcc
    28321 gcaacctttt ccccaaccat cccgccacac acggcgcgat gcgtcccgac agcgtatgcc
    28381 gtctgatgcc gaaaaaagac agttcggtgc aaggcaaagt acacgaaacc gtacaacccc
    28441 cctaccccaa acgccgtctg aagcatttta tgtaccatta cacgtacgac aactgggaac
    28501 aatatttcaa caagttcaac aaatatactt ccatttcagc cgaaaaatac cgagagcagg
    28561 gaaagcccgt gcgtttcgtt agggacatta tcctccgccc gatttggggg tttttcaaaa
    28621 tttatatcct gaacaaaggg tttcttgatg gaaaaatggg ttggattatg tccgtcaacc
    28681 acagctatta cacgatgatt aaatatgtca aactatatta tctgtacaaa tccggcggaa
    28741 aattttaaat ggaaaaagaa ttcaggatat taaatatcgt atcggccaag atttggggtg
    28801 gaggcgaaca atatgtctat gatgtttcaa aagcattggg gcttcgggac tgcacaatgt
    28861 ttaccgccgt caataaaaat aatgaattga tgcacaggcg attttccgaa gtttcttccg
    28921 ttttcacaac gcgccttcac acgctcaacg ggctgttttc gctctgcgca cttacccgct
    28981 ttatccggaa aaaccgcatt tcccacctga tgatacacac cggcaaaatt gccgccttat
    29041 ccatactttt gaaaaaactg accggggtgc gcctgatatt tgtcaaacat aatgttgtcg
    29101 ccaacaaaac tgatttttat caccgcctga tacagaaaaa caccgaccgc tttatttgcg
    29161 tttcccgtct ggtttacgat gtgcaaaccg ccgacaatcc ctttaaagaa aaataccgga
    29221 ttgttcataa cggtatcgat accggccgtt tccctccctc tcaagaaaaa cccgacagcc
    29281 gtttttttac cgtcgcctac gccggcagga tcagtccgga aaaaggattg gaaaacctga
    29341 ttgaagcctg tgtgatactg catcggaaat atcctcaaat cagactcaaa ttggcagggc
    29401 acggacatcc ggattatatg tgccgcctga agcgggacgt atctgcttca ggagcagaac
    29461 catttgtttc ttttgaaggg tttaccgaaa aacttgcttc gttttaccgc caaagcgatg
    29521 tcgtggtttt gcccagcctc gtcccggagg cattcggttt gtcattatgc gaggcgatgt
    29581 actgccgaac ggcagtgatt tccaatactt tgggcgcgca aaaggaaatt atcgaacatc
    29641 atcaatcggg gattctgctg gacaggctga cacctgaatc tttggcggat gaaatcgaac
    29701 gcctcgtctt aaaccccgaa gcaaaaaacg cactggcaac ggcaggacat caatgcgtcg
    29761 ccgcccgttt taccatcaac cataccgccg acaaattatt ggatgcaata taagccgctt
    29821 tcagacggcc tatgccgtct gaaagccttt gatgcaacaa accgccaaat tatattcgtt
    29881 cattggaaag aaacaccccg aatccatcct tcaaaataag aaaatcccaa tatctcccaa
    29941 tattacgcag cctattggca aagttttgca gcgtcttccc cggcttgtgc tgccgcatca
    30001 agtgctttgt tacaatgtat agtggactaa caaaaaccag tacagcgttg cctcgcctta
    30061 gctcaaagag aacgattctc taaggtgctc aagcaccaag tgaatcggtt ccgtactatc
    30121 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta tactatcttc
    30181 acatttctta ataaatttta tgagtaacca tacttcttgg tcgtccaaaa tcggtttcgt
    30241 acttgctgcg gcaggttcgg ccatcggttt gggcgcgatt tggaaattcc cttatacggc
    30301 gggcaccaac ggcggcgcgg tgtttttcct gctgtttttg atatttacta tcttggtcgc
    30361 cctgcccgtt cagcttgccg aattttatat cgggcgcacg ggcggtaaaa atgccgtcga
    30421 ttccttcagg gctctgcgtc cgagtacgca atggctttgg gtcgggcgca tgggcgtttc
    30481 cgcctgcttt attttgctgt cgttttacag cgtggtcggc ggatgggtat taaattatgt
    30541 cgtccacagt ttcacggggg cggttcatac cggcgcggac tttgaagcct tgttcggcgc
    30601 gacgatttcc aatccggcag gttcgctgtc ctatcaggca ctgtttatgc tgattacggt
    30661 ttgggtggtc aaaggcggca tttcagacgg cattgaaaag gcaaaccgtt atctgatgcc
    30721 ggggctgttt atcctcttta ttgcgctggc aatccgttcg ctgacgctgc cgggtgcaat
    30781 ggagggcgtg tctttcctgc tcaaaccgaa ttggtcgtac tttaaagccg atacgatgat
    30841 tacggcttta ggtcaggcgt tttttgcctt gagcatcggc gtttccgcta tgattaccta
    30901 tgcttcgtat ttgggaaaag atcaggatat gttccgctcg gggcatacga ttatgtggat
    30961 gaacctcttg gtttcgctgt tggcaggttt ggtgattttt ccggcggtgt tcgccttcgg
    31021 ttttgaaccg agccaggggc cgggattgat ttttatcgta ttgccggcgg tgtttatgaa
    31081 aatgccgttc ggtacggttt tgtttgcggt atttatgctg ctggtcgttt tcgccacgct
    31141 gacttcggca ttttcgatgt tggaaacggt cattgcctca accatccgcc aagacgagcg
    31201 caaacgcaaa aaacatactt ggcttatcgg cacggctatt tttattgtcg gcatcccgtc
    31261 cgcgctgtct ttcggcgtat ggggtgagtt taaggttttc ggcaaaacca tttttgattt
    31321 gtgggactat gttatttccg ccgtcattat gcctgtcggc gcgttgggcg ttgccgtatt
    31381 taccgcctgg attcaggaca agcagtctgt gttaaaagat gccggcgcgg gcagcaccgt
    31441 accacgggca gtgctgctgc tgtggctgaa taccttgcgc taccttgccc cgattgccat
    31501 tattattgtt ttcgtcaatt ctttggacat cctttaaaag ccatccaaac agcaaaaatg
    31561 ccgtctgaaa gcctttcaga cggcattttt gcttcaggtt cagcctattt cgttcaaa
//
LOCUS       DAWHRA010000003        30665 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWHRA010000003 DAWHRA010000000
VERSION     DAWHRA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30665
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.003"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..133)
                     /locus_tag="WIB30_000113"
     CDS             complement(<1..133)
                     /locus_tag="WIB30_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796261.1"
                     /translation="MPLLFLFLIAMVVRNVTLPGAMEGVAFYLKPDFSKITAELFVFV
                     "
     gene            complement(336..551)
                     /locus_tag="WIB30_000114"
     CDS             complement(336..551)
                     /locus_tag="WIB30_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ3796262.1"
                     /translation="MFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPIA
                     KIWKLVGWMGCSARSASWLITWYSAAG"
     gene            complement(506..640)
                     /locus_tag="WIB30_000115"
                     /pseudo
     CDS             complement(506..640)
                     /locus_tag="WIB30_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            960..1066
                     /locus_tag="WIB30_000116"
                     /pseudo
     CDS             960..1066
                     /locus_tag="WIB30_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1268..1960)
                     /locus_tag="WIB30_000117"
     CDS             complement(1268..1960)
                     /locus_tag="WIB30_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ3796263.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHSLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            complement(2028..4004)
                     /locus_tag="WIB30_000118"
     CDS             complement(2028..4004)
                     /locus_tag="WIB30_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ3796264.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWGELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMCIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4183..4848
                     /locus_tag="WIB30_000119"
     CDS             4183..4848
                     /locus_tag="WIB30_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ3796265.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            4900..5727
                     /gene="mutM"
                     /locus_tag="WIB30_000120"
     CDS             4900..5727
                     /gene="mutM"
                     /locus_tag="WIB30_000120"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ3796266.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPRCGGLVVKETLGQRGTFYCPNCQK"
     gene            5815..6582
                     /locus_tag="WIB30_000121"
     CDS             5815..6582
                     /locus_tag="WIB30_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ3796267.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIRV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(6893..7204)
                     /locus_tag="WIB30_000122"
     CDS             complement(6893..7204)
                     /locus_tag="WIB30_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ3796268.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
     gene            7733..10012
                     /gene="nrdA"
                     /locus_tag="WIB30_000123"
     CDS             7733..10012
                     /gene="nrdA"
                     /locus_tag="WIB30_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ3796269.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            10070..11083
                     /gene="dcm"
                     /locus_tag="WIB30_000124"
     CDS             10070..11083
                     /gene="dcm"
                     /locus_tag="WIB30_000124"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ3796270.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            11076..12263
                     /locus_tag="WIB30_000125"
     CDS             11076..12263
                     /locus_tag="WIB30_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ3796271.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            12324..13457
                     /gene="nrdB"
                     /locus_tag="WIB30_000126"
     CDS             12324..13457
                     /gene="nrdB"
                     /locus_tag="WIB30_000126"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ3796272.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            13558..13674
                     /locus_tag="WIB30_000127"
                     /pseudo
     CDS             13558..13674
                     /locus_tag="WIB30_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            13691..13981
                     /locus_tag="WIB30_000128"
     CDS             13691..13981
                     /locus_tag="WIB30_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ3796273.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLVGEDEGLSDSLSK"
     gene            complement(14022..14300)
                     /gene="ftsB"
                     /locus_tag="WIB30_000129"
     CDS             complement(14022..14300)
                     /gene="ftsB"
                     /locus_tag="WIB30_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ3796274.1"
                     /translation="MKWVTVVLSVSLVCCQYSLWFGKGSIGRNNNLKNQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(14314..15600)
                     /gene="eno"
                     /locus_tag="WIB30_000130"
     CDS             complement(14314..15600)
                     /gene="eno"
                     /locus_tag="WIB30_000130"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ3796275.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMTLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLIVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTSAEFAEYLEGLV
                     NEFPIVSIEDGMDENDWEGWKLLTEKLGSRVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            complement(15647..16087)
                     /locus_tag="WIB30_000131"
     CDS             complement(15647..16087)
                     /locus_tag="WIB30_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ3796276.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKDAV"
     gene            complement(16102..16944)
                     /gene="kdsA"
                     /locus_tag="WIB30_000132"
     CDS             complement(16102..16944)
                     /gene="kdsA"
                     /locus_tag="WIB30_000132"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ3796277.1"
                     /translation="MDIKINDITIGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            complement(16966..17349)
                     /locus_tag="WIB30_000133"
     CDS             complement(16966..17349)
                     /locus_tag="WIB30_000133"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ3796278.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            complement(17498..21637)
                     /gene="mfd"
                     /locus_tag="WIB30_000134"
     CDS             complement(17498..21637)
                     /gene="mfd"
                     /locus_tag="WIB30_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ3796279.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHALPDLAVNRQSDEPLQALKDFQTAFEGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESRLPPGKSNLHGQIQQQPTPSP
                     VGEGWGEGKAVAAQTEFSAAAISPLPSPLPSPLPQEREQSAAAVSDGLKAKSSLHLVA
                     SNLHGQIRQQPTPSPVGEGWGEGKAVAAQTEFSAAATNPLPSPLPQEREQSAAVVSDS
                     LKAAAVSTESSLPPGKSNLHGQIQQQPAPSPVGEGWGEGKAVAAQSAIAVITESDLYQ
                     YVARSRVHNRRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLVTMDLGGETNE
                     MMLLEYAGEAQLYVPVSQLHLISRYSGQAHENIALHKLGSGAWNKAKRKAAEKARDTA
                     AELLNLYARRAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMD
                     RLVCGDVGFGKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVA
                     SLSRFNNSKATKAALEGMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQK
                     EQLKRLRANVDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGS
                     VREAVLRELKRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMR
                     DFLQQRFNVLLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAY
                     LLTPEYITKDAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFT
                     LYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLA
                     VCETVQQINAIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTF
                     GKNNNVDPTEIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLP
                     K"
     gene            21822..23249
                     /locus_tag="WIB30_000135"
     CDS             21822..23249
                     /locus_tag="WIB30_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ3796280.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLGRYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AKHEDAAACLLNDIRKDILDCRYCG"
     gene            23291..23542
                     /locus_tag="WIB30_000136"
     CDS             23291..23542
                     /locus_tag="WIB30_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796281.1"
                     /translation="MPLDGIFGGGRQISGGAKGNARHSAKPPIAADTAFRAAPLRRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            23451..24560
                     /gene="mltB"
                     /locus_tag="WIB30_000137"
     CDS             23451..24560
                     /gene="mltB"
                     /locus_tag="WIB30_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ3796282.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(24683..26689)
                     /locus_tag="WIB30_000138"
     CDS             complement(24683..26689)
                     /locus_tag="WIB30_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ3796283.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            26978..29005
                     /locus_tag="WIB30_000139"
     CDS             26978..29005
                     /locus_tag="WIB30_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ3796284.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGNLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(29096..30649)
                     /locus_tag="WIB30_000140"
     CDS             complement(29096..30649)
                     /locus_tag="WIB30_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ3796285.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
BASE COUNT         7092 a         7126 c         8679 g         7768 t
ORIGIN      
        1 aaacgaagac gaacagttcg gcggtaatct tgctgaaatc aggtttcaga tagaaagcaa
       61 ccccttccat tgcgcccgga agggtaacgt tgcggacgac catcgcgatt aggaacaaaa
      121 acagcagcgg catcagctat tttgccgctt tttcaatgcc gccgataacg cctttgacca
      181 aaatccattg gttcacggcg acaaaaagca gcgtataaaa cgcaatttcc caagggctgt
      241 ttcaatgtgt tcggcaaaga agccttttgt aaccacaccg tcgacggggc tggaaatatt
      301 caaatttcct ccaataatat taacgatata gctgattacc cagccgccga gtaccatgta
      361 ataagccatg atgccgaacg cgccgagcag cccatccagc cgaccagttt ccaaattttg
      421 gcaatgggtt tgccgttcat cgggccgccg aacgcatcca gcgcgttcac gcctttgcgc
      481 cgtccgatga cattttccac caaaatcatc gggatgccga taaccagcat cgcgatacag
      541 aataaaaaca catacgcgca ccaccgtttt caccgaccaa atacgggaaa cgccacgtcg
      601 cgccgaaacc gacagtcgcg ccggcaacgg tcaggatata ggttaatcgg ctggaccagg
      661 tttgacgatt ggtatttgaa ggggaagaca tattgaaacc gtgtccgatt gagataaagc
      721 ggaaattcta cacgcgtttt ttaacaggaa ctgactgatt gcttttcaac tgtctagtga
      781 ttttccatgt aaaaggcata tatttcagtg agtatttctg atgagtataa cccgatgagg
      841 aagaatcgga gtttataaat agattaattt gttattcttc tacatcggtg tatggaaatg
      901 aaattttgtt aattatatta atgatagcat ttaaataatg atgaaagaga gggaatttga
      961 aatatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt
     1021 acggcaaggc gaggcaacgc tgtactggtt taaatttaat ccactataaa aaaaccatct
     1081 atacaagggg agaagtatag atggcaaaac acattacggg gaaaacgtct tactcataag
     1141 cctgcttgaa caggcgttac tcagacaaat ggattatatc gtaaattgat tttttgcgtt
     1201 aaattgggtt aaaccatata tttaatgagt atgtggatga atataaatgg aattcttgga
     1261 tgttatttca gaattttgga cattcgataa atttccattc cccgggtttg aggttttctg
     1321 tttccagatg tgcgaatcgc cggcgatgaa ggtgttgcac gcggttgccg gcggcggcga
     1381 tcatgcgttt gacttggtgg tattttcctt cggtaatggt cagcagcagg gtggtcgggt
     1441 ttttcaaaac ggcatcggcg gcacaaacgg tttcgttttc gtcgtggagc agcacgccgt
     1501 ttttcaaggt ttcgcagagc gtttctcctg tggggtgttt gagcgttact tcgtacagct
     1561 tgggaatttt tctgctcggc gaagtcaggc tgtggttcag tttgccgtcg ttggtaatca
     1621 gcaatacgcc ggtcgtatct gcatccagcc tgccgaccgc ctgcatatcg atgttccgca
     1681 tattgtcggg gaacaggctg aatacgctgc ggtagtgctt gggtttgtgc gaagtttcgt
     1741 aatcttcagg cttgttgagc atgatgtaga aatagggttc gggaacgacg gttactgctt
     1801 ccccgtcaat atccaacgtt tcgacggatg aggaatcgat gtctgcatcg gtgtcgtcca
     1861 tgcaggttcc gttgatgaaa acataaccgc cggcaatcag ccattggcac tgcttgcggc
     1921 ttcctatgcc ttgatattgc aggtatttga taagtttcat gatggtatgg gaaatgtggg
     1981 atgaaaaaga caggactgag aaagtcctgt catgtgccgt atccgaatca gcttccaatc
     2041 agtttgaccc tctgtcccgg attgatggtg ttcaggttgg gattgagccg gcggatgtcg
     2101 tcgatatgga tattgaagcg cgcggcgata cttttgaagg tgtcgccttt gcgcgcggtg
     2161 taggatactt tttcaatacg ggtttgggca ggggctgcct gcgccaggcg gagtacctgt
     2221 cctttttgga tggtgttgcc tttgatgttg ttggcgacaa tcaggtcggc tacactgacg
     2281 ttgtagcgtt tggcaatgtt gaacagggtg tcgccttcta caacgcggtg gatgctggca
     2341 tggagcgggg agatttcggc ttcgccgtcg gcagctttgg ctacgcgcgt ttccaaacgt
     2401 gcccggcgtt tgccgtcggc ggcaacggta tgttgcggag atgaggaaat gttgtgttcg
     2461 gcaactgtgt caggcgtgcc gatgacggcg gagatggttt cttcagcctg tcggcgcagg
     2521 ttgtttcggg caacaagctg catgagttcg tctgccgcct gcatgtcgtt ttgggggatg
     2581 atctgcgcga gtgttgcggt ttgggtttca gacggcatgg cggtctgttt ttcggtttga
     2641 ggttgcgctg cggctgtcgc aagggcaggt tcggcaatac ggacaagggg atcgggttcc
     2701 gtacggacgg ttttctgcgg caaaggtgcg acggtaatgt ccgctgtctg tgcggcggcg
     2761 ggtcggattc gggcaatgcc gacgttcacc gtgcctgccg gcatattgga acggtaggtg
     2821 tcgggcgtat tgtcgatgtc gatggaaacg acggattccg atgccgtctg aagggttttg
     2881 ccgttcttgg cgacaaggat gctgcgtcct gcgttgacaa ggttgccgtt caggttgttg
     2941 aggcgtttga tgtcggcaat gcacatgccg gttgccgtcg agatgtcgga caggctggtt
     3001 ttggcggcag gcgtatagac ttcccatgaa aacaggctgt cgggtgcggc gttgaggtag
     3061 ttgctttgga aggtttgtac ggacgcgaca ggaagcagca gtttgcgttt gcttttgggg
     3121 ataaacgcgg ggacgttgaa tgcggggttt agggcgagca gctcgctttg cgtgatgccg
     3181 gcaagccggg cgatggcttc gttgtcgagc ggacgatccg gttcgactgc ctgaaaatag
     3241 ggtttgttgt ctatgtcgct gatattcatg ccgaaagatt ggggagtggc aataatgttg
     3301 cgcacggcga gcagcttggg gacatagttg cgcgtttcgt tgggcatacg caggttttcg
     3361 taggtcggtt cgagcccttg ggcgcgggcg cggttgatgg cgcgtccgac gttgccttca
     3421 ccccagttgt aggcggcaaa ggcaagcggc cagtcgccga acagtccata gaggtattgc
     3481 agatagttga gtgcggcatc ggtggcggcg taaacgtcgt gcctgccgtc gtaaaccggt
     3541 gttttttcca ggccgtaatg cctgccggta gcgggcataa actgccataa tcctgatgcg
     3601 ccgacgtgtg atttggcttt ggtgacgaac gcgctttcga tgaagggaag cagggcggct
     3661 tcggcgggca tattgcgttt tttgacttcg ttggcgatat ggtacatata gggtctactc
     3721 cggttgatga ccctgttgaa atagctgtgg cttgcgatga atttgctttc gtggcggcgt
     3781 accagttcgg gattgacttc gcccatccgg aagccttggc gcagctcgcc ccacaggctg
     3841 ccggattgga aatattgttt tgtcgggggc aggtcgagta ttgaagagtt taagcgcata
     3901 atcgccaaac cgatttggtg tgatgaggtg ttttgggcgt ataggaaacc cggacaaacg
     3961 gacagacctg atgcggtcag agcgatggtt ttgagtttgg acatgattta tattcggcgg
     4021 aataaacata ttttatcgtc cgatggtact gtttgactga aaaagcgtca agttttacag
     4081 gaactttatg cggattcgga aatgggcggc cgatgtgttt tttgacagaa atcctgtttg
     4141 gaatagaata tcggcaggca atctgtttga aacggacgca atatggattc atggtttgaa
     4201 gatacggcga tggggcggta tgttgcaaaa ttggaacagg atttcttcgg gcggtatctg
     4261 gattcatacc gtttttcggg aatgtgtgcg gttcaggtgg gcggtccgtg gctgagcctg
     4321 tctgaagatg ttgtctgtgt gccgcgagat atgtcgatgt cggcggagaa tatggctttg
     4381 gcggatgttt ctgcggatat gctgcttttt ccgcatacgc ttgaaggcgg tgttccttcg
     4441 caaatcctgt cggaagcgca ccggatattg aaaccgtgcg gacgcttgat gctgacgggt
     4501 ttcaatccgt attcgctctg gggattcagc cgttggtttg acggcgaacg cctgccggaa
     4561 aaacggtttt gtctgccgct gcccgagttg aaaagacggc ttgcggatgt cggtttcgat
     4621 attgaatttg ggaaatttat ggtgtatctg ccgccggttt cgtcgctcgg gcaaatacgc
     4681 ttttggcggt ttatggaaaa ggcgggcgac cgttggtggc cgcagtgtgc cgcagtgtac
     4741 ggtttggttt tggtcaaaag ggcagcgggc gtaacgcccc tgcccgcgtg ggacgggtat
     4801 ttgggcggca aagcccttgc ggcgggtgcg gcaagggttg cggattaggg acagccgtct
     4861 ttcagacggc atctgtcaga agaaatgtcg ggaggggtta tgcctgaatt gccggaagtg
     4921 gaaacgacgt tgcgcggcat cgcgccgcat attgaaggga aaacggtgga agccgtggta
     4981 ttgcgccaat tgaagctgcg ctggcagatt aatcccgatt tgggggagat tttgtccggc
     5041 cggcaggtgt tgtcctgcgg caggagggcg aaatacctgc ttatccgctt tcaaacgggc
     5101 gtgctgctga ttcacttggg gatgtcgggc agcttgcgga tttttacgcc gtcggacgga
     5161 cgtatcggca ggccggacag acacgatcac gtcgatattg tgttttcaga cggcacggtc
     5221 atgcgttacc gcgatccgag aaagttcggc gcgatacttt ggtatgaggg aatcgaagaa
     5281 catcatccgc tgttggaaaa actggggccg gagcctttgt cggaggcatt ttgtgcggat
     5341 tatctgtatg caaggctgaa ggcgcagaag cgcgcggtca aacttgccct gatggacaat
     5401 gcggtcgtgg tcggtgtggg caacatttat gccaacgaga gcctgttcag agcgggcatt
     5461 tcgccccacc gtcctgccaa ccgcctgaaa aagaaagagt gcgcgctttt ggttgaaacc
     5521 gtcaaagcgg tgttgcagcg cgccattgaa acgggcggca gtaccttgag ggattttgtg
     5581 gacagcgacg gcaaaagcgg ctacttccaa caggaatata cagtgtacgg gcggcacaat
     5641 cagccgtgcc cccggtgcgg cggtttggtt gtgaaagaaa ctttggggca gcgcggcacg
     5701 ttttattgcc cgaactgtca gaaataggac tgaaaacggt ttcagacggc attttatcgg
     5761 tatgccgtcc gaacgtttca acaacaaaca ccgattatcg ggaaagaatt gctcatgtct
     5821 tcaaataaag cttcattttt tacacgtctg cgccgcttgt gccgtttggc ggtctggctg
     5881 ttcaaaaccg ggaaaaacct gcgcggtatt gacggcggtt gccccgagtc gcgcaatcgg
     5941 gcggtaatcg agttgggcag gggggttttg gcggctttgg atatcggatt ggaggtgggc
     6001 agacccgcac ccgaacatcc gaacggtgtc ttggttgccg ccaaccacgt gtcctggctg
     6061 gatattttcg cgatgagcgc ggtttatccg agcagcttta tcgccaagca ggaaatcaaa
     6121 agctggccgg tattgggcaa gatggggcag aacgcgggaa cggtgttcat caaccgcaat
     6181 tcgcggcgcg acatcgaacc gattaaccgc gccgtctgcg aaaccttgca acgcggtcaa
     6241 aacgtcagtt ttttccccga agcgcggact tcctccggat tggggctttt gccgttcaaa
     6301 gccgcgctgt tccaatccgc catcgatgcg ggggcaaagg ttttggcggt cgcgctgcgt
     6361 tattatgacg aaacgggaaa aaggacggct cgtccctcat atgccgatgt cggtttgccg
     6421 acctgcctgt ggcgcatcgt gtctatgaaa aaattgacga taagagtcga tttcgtttgc
     6481 gtggcggatg cggcggaaag cgaagaccgt tatgctttaa aagataaaat cgaagaaagc
     6541 atccgtgccg ttgtcgccga cgatgcggat atcgctgtct gaaaccggtt gtcggaatgt
     6601 ggcagtatga ttcgcttttg tggatgtata gtggattaac aaaaaccagt acagcgttgc
     6661 ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt gaatcggttt
     6721 cgtactatct gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
     6781 attaaggctg cggcaacggg aggacctcaa acgaaatggc gtaaggatag tttgttgtat
     6841 ctatggaaaa tgccgtctga acctgtgttc agacggcata ttttatatgg gattaacggc
     6901 ggaaactgcc gccgtcgaaa tcatcgaaag cctcataagc cgcccggcat tggattttct
     6961 gttccgcata aggcagggag cggaatgctt tgcgcgcctt tttgttttcg cgcaattcct
     7021 gcacgttgcc ttgcgatcgg taccacgcgg cacggcgttc caaatatttc ctgcattcgg
     7081 ggtgcagcat ctgcttgttt gccttgcgga tatgtttggt atcgcggtgg gcgggttctg
     7141 acgtaccggc agcggcagtt gtcgatacca gtcccaatat tgcagacaac agtatggcaa
     7201 gcatcttttt gacagacatg acgatccttt cagtttgtca ttctttccag tataggatgg
     7261 aaggatggaa cgggcaaatt gtcggcagtt tcagactcat tcagccgaca aaacttgccg
     7321 tttgatatgc agattcaaat aacacattaa tctcaataat aaattccaat cggtaagaaa
     7381 atggaatttg tcggcggcgg cgcggcaaaa tcatactttg caaaatttaa caatttgcag
     7441 gggcagaaaa caggaagctt tcctttttcg tcggaaaatc cttatttcac cgccttgtag
     7501 ccggagccgg tcaaaaggca aaaaatttac ccgtttttta tcggtaaaga attatcagat
     7561 aaaacaaata ttataggaaa aatacgacag gcgggtttta tcgcgcattg cctgaaactg
     7621 aaaaatacaa ccgttgtcaa gactggagaa aatgccaaaa atccactata ttgtctgcct
     7681 taatttattt gaaaagactg tgtcttgaat atcaagagtg gaagaggaag cgatgaatac
     7741 accgactgat ttgaaagtaa ccaaacgaga cggaagatta gaagccattg atttggataa
     7801 gattcaccgt gtcgtcactt gggcggcgga cggattggaa aatgtttccg tgtcgcaggt
     7861 cgagttgaaa tcgcacatcc agttctacaa cggcatccgc accgacgaca tccacgaaac
     7921 catcatcaaa gccgctgccg atttaatttc ggaagatacc ccggactacc aataccttgc
     7981 cgcgcgtttg gcgattttcc atcttcgtaa aatagcctac ggcgagtacg agccgccgca
     8041 cctttacgac cacgttaaaa aacttaccga tgccggaaaa tacgacaggc atatccttga
     8101 ggattacagc cgcgaagaat ttgacgaact gaacgcctat atcgaccacg aacgcgatat
     8161 gtccttttcc tatgccgctg tcaaacagct cgaaggcaaa tatctggtac agaaccgcgt
     8221 tacccgccaa atttacgaaa cgccgcagtt tttatatgtt ttggtggcga tgtgcctttt
     8281 cagcaaatac ccgaaagagg cgcgcttggg ttacgtcaaa cggttttacg atgccgtttc
     8341 tacatttaaa gtatcgctgc cgactccgat tatgagcggc gtgcgtacgc ctacgcgcca
     8401 gttctcaagc tgtgtgctga ttgaatgcga cgatagtttg gattccatca atgccactac
     8461 cagcgcgatt gtgaaatacg tttcccagcg tgcgggcatc ggcatcaatg ccggacgtat
     8521 ccgcggtttg gacagcgaaa tccggggcgg cgaagcgcgg cataccggct gcattccctt
     8581 ctttaaaatg tttcaggcgg cggtcaaatc ctgttcgcaa ggcggcgtgc gcggcggcgc
     8641 ggcaaccttg ttctacccct tgtggcatat cgaagccgaa agcctgctgg tgttgaaaaa
     8701 caaccgcggt gtggaagaca accgtatccg tcagcttgat tacggcgtgc aaatcaaccg
     8761 cctgctgtac acccgcctga ttaagggcgg caacattacg ctgttttcgc ccaacgaggt
     8821 tccgggattg tacgaagcgt tttttgccga ccaagacgaa tttgagcggc tctatacgaa
     8881 atacgagcaa gaccctgata tccgcaagcg catcattccg gctgccgacc tgttttccac
     8941 gctgatgcag gagcgtgccg gaaccgggcg catctacatt caaaacgtcg atcactgcaa
     9001 tacgcacagc ccgttcgatc cgcgcgtcgc gcctgttcat cagtccaact tgtgtatgga
     9061 aatcgccctg ccgaccaaac cgctggacaa tatcaacgat ccgaacggcg aaatcgccct
     9121 gtgtacactg tctgccttta acttgggcgc attaaacagc ttggacgaat tggaagggct
     9181 tgccgacttg accgttcgtg cgctcgatgc acttttagat tatcagggat atccggtaga
     9241 agccgcgcgt acctctacta tgggccgccg ttcgctcggc atcggcgtga ttaactatgc
     9301 ttattatctg gcgaaaaacg gtgtccgcta cagcgacggt tccgcgctcg gtctgaccca
     9361 ccgtaccttt gaagccatac agtattacct gctcaaagca tcggcaaacc ttgccaaaga
     9421 atacggtgcg tgcacgctct ttaaccaaac cgtttattcg caaggcaaac tgcccatcga
     9481 cacttacaaa aaagatttgg atgccgtctg cggcgagcct ttgcattacg actgggaaag
     9541 cctgcgtgcc gaaatcgtca aatacggcct gcgcaactct actctgaccg cgctcatgcc
     9601 gtctgaaacc agctctcaaa tcgccaacgc caccaacggc atcgagccgc cgcgcggatt
     9661 ggtaacggtc aaagcatcga aagacggcat tttgaaacaa gtcgtgccgg agtttgaaac
     9721 cctgaaaaat gcctatgaaa ccctgtggca gcttcccggc aacgaaggct acctgaaact
     9781 tgtcggcgtg atgcaaaaat tcgtcgatca atcgatttcc gccaataccg cctacgaccc
     9841 gggcaaattc gaaggcggca aagtttctat gaaacaaatg ctcaaagacc tgctgaccgc
     9901 ctacaaatac ggcgtcaaaa ccctgtacta ccataacacc cgcgacggtg cggacgatac
     9961 gcagaccgat attcaggatg acggctgcgc gggcggggct tgtaagattt gatgaaaggg
    10021 ggagttttca gatggccttt agattaataa atcatctgaa atataaaata tgaaaaataa
    10081 aatacaaaat caattagata atatagttac cttaaataat gagaaattta cttttattga
    10141 tttatttgcg ggaataggtg gttttcgcat tgcaatggag aatgttggtg ggcgatgtgt
    10201 attttctagt gaatgggatg ataaagcccg tcaaacctac caagtaaatt ttaatgatat
    10261 tccttatgga gatattacat taaaggaaac caaagcagct attccaagta agtttgatgt
    10321 attaacagca ggattcccgt gtcagccatt ctctatagct ggtgtttcaa agaaaaaaag
    10381 cctaggacga gaaacaggct tcttagataa ggcgcaggga actctatttt ttgatgttgc
    10441 tgaaattatt ggaaaacatc gacctaaaat ttttctttta gagaatgtga aaaaccttgt
    10501 ttcgcatgac aaaggaaata catttaaagt aattaaaggg actttagaag agcttgacta
    10561 tcagatattt tatcaagtta tgaatgcaaa atattatgtt cctcaaaata gggagcgtat
    10621 ttttattgta ggttttgata gacaatattt taataaggaa ataaatttca attttccttc
    10681 cccaccagaa tcacaaccaa aattaaagca aattttggaa gatgatgtag ataattcttt
    10741 tactctttct gataacttat ggctttacct tcaaaattac gctaaaaaac ataaggcaaa
    10801 gggtaatgga tttggttttg gattagttga tttagatgga atatcacgaa ctctatctgc
    10861 acgatattac aaagatggtt ccgaaatact catccctcag aaaggaaaaa atcctaggaa
    10921 gctgacacct agggagtgct cgcgtttaat gggatttcct aaagattttg ttattgatgc
    10981 agtatccaag acagctgcat acaagcagtt tggcaattca attgctgtac cgttggttca
    11041 agctattgct aaacaaatta taaatgagtt aaaaaatgaa tgacttggca gctcagacaa
    11101 tacaagtagt aaataaatcc gatgacttag tagcttcagc aatacagaca gcaaataaat
    11161 caattgcagt atactgtcgt tatattcgtc ccaatgatgt tggtactact ggtagtcacc
    11221 aatcaggatt ttatattcaa aaacattttt cagacgacct ctttgatgta gtttgccaaa
    11281 agggaacaaa taaaactatt tcaatcaaaa ttaattggca agatggaagc gtcactaata
    11341 gcaattttaa atattacggc caaggcacga gaaatgaggc gagaattacg ggttttggca
    11401 agaattttga atttttaagt gataaatata gtggttcctt attggtattg tgcagagctt
    11461 gttataaaga tttattattc catgcttttg ttttatcttc agatgaagat attgaaatat
    11521 ttatcgcaga aactaacatt ttaccaggaa gtttatattt acctaaaaaa caagaagtag
    11581 aggataacct aacaaaatta ttttcactat ttcctaactt ccccaaaact gaagaaatgg
    11641 cagttttggc aagggaaaat attgcattaa ataaaactaa cgttagtaat gtgttaaaac
    11701 aatgggtttc aaaggaatat gaattatttt ccatttttga aaaacgtgaa tttgaaatta
    11761 ttaaatcaaa aattactgat ttggatagct tcattaattt tgcacattct tttacaaatc
    11821 gcagaaaagc aagagcagga aaatctttag agttacattt atcaagaata tttgacgaat
    11881 tttccttaaa atttgagacg caagcgaaga cagaagggaa aaagaaacca gattttttat
    11941 ttcctggaag tgaagagtac catgcgatgg atgaatcagg gaatttcata tttcaaactg
    12001 aaaagctgac aatgctaggt tcaaagacta cttgtaagga tcgctggcgc caagttttaa
    12061 atgaagcaga tcgaattcct cataagcatt tatttactct acaagaaggt atatctgata
    12121 ctcaaattca ggaaatgagt gatgagaatt taaccttagt tgtccctaaa gaatccgtaa
    12181 aaacatttgg aacttttggg aaaacccatg ttttaacttt agaaaatttt attaaatata
    12241 taaagtctca gcaggttagt taaaacttac agatcatttt taattaatat agagaataga
    12301 ttatgtcctg cgaacactta actatgtcat acagcacctt tcccaaaacc aaaaacgacg
    12361 cgctgaatga gccgatgttt tttggtcagc cggttaatgt tgcccgttat gaccagcaga
    12421 aatacgaagt atttgaaaaa ctgattgaaa aacaattgtc tttcttctgg cggcctgaag
    12481 aaatcgatgt ctcgcgcgac cgtatcgact acgccaatct gcccgaacac gaaaaacata
    12541 ttttcatcag caatctgaaa taccaaacac tgctcgattc catccaaggg cgcagtccga
    12601 atgttgcctt gctgcctttg gtttcgattc ccgagttgga aacgtggatt gaaacgtgga
    12661 gcttcagcga aaccatacac tcgcgcagct atacccacat catccgcaat attgtgaatg
    12721 atccgtcggt cgtgtttgat gatattgtcg aaaacgaata cattaccgcc cgcgccgaag
    12781 acattgcctg ctattacgac gacttaatcg aatacaccca gtattacaat ctgttgggcg
    12841 aaggggtgca caatgtcggc ggaaaaccca ttactgtgtc tttgcgcggg ttgaagaaaa
    12901 aactctatct ctgcctgatg tgcgtcaacg tgttggaagc tatccgtttc tatgtttcat
    12961 tcgcctgctc gtttgctttt gccgagcgcg agttgatgga aggcaacgcc aaaatcatca
    13021 aactgattgc ccgcgacgaa gccctgcacc tgaccggcac gcagcatatg cttaatctga
    13081 tgcgttcggg tgttgatgat tctgaaatgg cagaaattgc cgccgagttg caggacgaat
    13141 gtttccaact cttcaaaaaa gcggcggaac aggaaaaaga atgggcggca tatttgttta
    13201 aagacggttc gatgattggt ttgaacaaag aaatcttatc ccaatacgtc gaatatatta
    13261 ccaatctgcg tatgcaggcg gtggggttgc cggccggatt tgaaggcgca aatcaaaacc
    13321 cgattccgtg gattaatgcg tggctgtcgt ccgacaacgt acaggtcgcg ccgcaggaag
    13381 tggaaatatc ctcttatttg atcggtcaga tagattctga agtgaatacg gatgatttgg
    13441 gcgattttga gttgtaatgt tttttgaaaa aatgccgtct gaagccggat gtttcagacg
    13501 gcatttgtat gttgtatgat gggaaaaaca gcgggtagga tcggttattg ttccttttat
    13561 agtggattat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
    13621 atagtacggc aaagcgaggc aacgccgtac tggtttaaat ttaatccact atatttttga
    13681 ttggtcggaa atggcgcgca ttggtacgaa taagggtttt tttgaactct tggagggcga
    13741 gactctgttg gaaggcttgg aacggacggg gcatatggtg gaatatcagt gccgaagcgg
    13801 atattgcgga tcgtgccggg tgaagatttt ggagggaagc gttacttatc gggagccgcc
    13861 tttggctttt ttggggcggg acgagatact gccgtgctgc tgttgcgtcg agggggatgt
    13921 caggctggat tgcggtttgg tgggggagga tgaggggctg tctgacagtt tgtcgaaata
    13981 aaagccgtcc gaacattggt tcggacggct ttttgacggt attaccggtt atgcctgatg
    14041 agtcggtaaa aggtttcgcc gtcttggata taacccagtt ctacccgggc gatttccgaa
    14101 atggcttctt gaccgttttc caaatcatag acttcggctg caagggaatg gttgcgtagg
    14161 gcgagtgtct ggtttttttc ttcctgaacg gcaatctgat ttttcaggtt gttgttgcgc
    14221 ccgatactgc ctttgccgaa ccagaggctg tactggcagc agacaagtga gacggataaa
    14281 acgacagtta cccacttcat acgttaacct tttttatttg cccagttggt agaatgcggc
    14341 tttcccaggg tagtaggcgg cttccgccaa ttcttcctcg atacgcagca gttggttgta
    14401 ttttgccatg cggtcggaac gggacaggga gccggttttg atctgcatac agttggtggc
    14461 tactgccaag tcggcaatgg tactgtcttc ggtttcgccg gagcggtggc tcattacgct
    14521 ggcgtagcgg ttacgtttgg ccaagtcgac ggctttcagg gtttcgctca aagtaccgat
    14581 ttggttgact ttgaccaata gtgcgtttgc cacgcctttt tcgatgcctt cggccaagat
    14641 ttttggattg gttacgaaca agtcgtcgcc gaccaattga actctgctgc ccaatttttc
    14701 ggtcagcagt ttccagcctt cccagtcgtt ttcatccatg ccgtcttcga tggaaacgat
    14761 tgggaattcg ttaaccaagc cttccaagta ttcggcaaat tctgcgctgg tgtaagagcg
    14821 gccttcggct tccaagtggt atttgccgtc tttgtagaat tcgctggatg cgcagtccag
    14881 ggcgaacaat acgtcttcgc ctgctttata gcctgcggct tcagttgctt cgacgatcag
    14941 ttgcagggct tctttgtggc tgttcaggtt aggtgcgaaa ccgccttcgt caccaacggt
    15001 agttgggaag cctttgctgt cgcacagttt tttcagtgcg tggaagattt cggcaccgca
    15061 acgcagggct tcgcggaaag attttgcgcc gacaggcata atcatgaatt cttggatatt
    15121 caggctgtta ttggcatgtt cgccgccgtt gataacgttc atcatcggaa caggcagggt
    15181 cattgggcct gcgccgccca agtagcggta aagcggcagg cctgagtctt cggcagcggc
    15241 gcgtgcaacc gccatagaaa ccgccaaagt cgcattcgca cccaaattgc ctttgttttc
    15301 agtaccgtcc aattcgatca tgatttggtc gatataagat tgctcgttgg catcgatacc
    15361 gatgagggct tgggcgattt ggttgttgac gtgttcgacc gccttcaata cgcccttgcc
    15421 ggaatagcgg gatttgtcgc cgtcgcgaag ttccaaagcc tctttttgac cggtggacgc
    15481 gccgctcggt acggctgcgc gtcccattac gccggattcg agcaatacat cacactcgac
    15541 tgtggggttg ccgcgtgagt ccaaaatttc gcgggcgaaa atatcaacga ttgcgctcat
    15601 gaatgttctc caagtgaagt gaagggaggt aaaaaatggg agggcttcag acggcatctt
    15661 tgccggaaag ccgccaactt aaaactgctg aaagtagggc ggttgccata aaaacaatac
    15721 tggaaatgcc gtgccacatt ccgaaggatc cgcctacaaa cgacaacagc caattttcat
    15781 gtccgtattt cagtgcctcg ataacgggtg taatcaagaa tcggttggcg gcaagggcgg
    15841 acaataaaaa aagcagcagg gcggtttgcc gccgggttag ggcggcaaag gcggcagcca
    15901 gtaccgcgcc ccaaacggca agcccgctcc aagagaggat gtcgaacaat acgccggcaa
    15961 tttcgcccgc ctgcattttg ggcagcattt tgaacagcac cggcgcgacg atataacccg
    16021 ccataatctg cataccgagc cacaaggttg cggcatacag gctgattttt cggaaagtct
    16081 gcatcataag gcgaaaccgt gttactcgat tgttaaaatc ggttgtgatt tgattaaatc
    16141 gtccaatgct ttgatgcgga ttaaaaaatc ttctaaaagg tgcagcggca gcgcgctggg
    16201 gccgtcgcat tttgccagtt tcggatcggg gtgcgattcg aggaacagac cggcaaggcg
    16261 ggttgccatg cctgcaagtg ccaaatccaa agcctgtgcg cgacgaccgc cggatgcggc
    16321 agaaccagca tcgcgggttt gcagggaatg ggtaacgtcg aaaataaccg gcaggttgcc
    16381 gcaggtttgt ttcatcacgc cgaaaccgag catatcgaca acgaggttgt cgtagccgaa
    16441 gctgctgccg cgttcgcata aaatcagttt cccgttgccg gcttcgtgga atttttccac
    16501 aatgtttttc atttgagagg ggctgaggaa ctgaggtttt ttgatgttga cgacgttgcc
    16561 agtttttgcc atggcaacca ctaaatcggt ctgccgcgca agaaaggcgg gaagctggat
    16621 gacatcgcac acttcggcga cgggttggca ctgatggggt tcgtgtacgt cggtaatgac
    16681 ggggatgccg aactctgctt tgactttttc aaaaatcttt aagccttctt ccaagcctac
    16741 gccgcgataa gaatggatgg aggaacggtt tgccttgtcg aaagaggctt taaagatata
    16801 gggaattccg agtttgcggg ttacttcgac gtaatgcgcg caggtttgga gggtggaatc
    16861 caagctttcc aaaacgttga tgccgccgaa taggacgaag ggcgagttgt tgccgatggt
    16921 gatgtcgttg attttaatat ccataatcaa tgtccgtttt cagggttaca gtacggtgtg
    16981 cggcggctcg taggagatga tgtcgcggat tttgttgttt ccgtctacca agacgacttt
    17041 gggctcgtgt gcggcgattt cgggttcgga gagttggacg taagacatga tgatgacgat
    17101 atcgcctttc tgcaccagcc ttgccgcagc accgttcaga caaatcacgc cgctgccgcg
    17161 tttgcctgaa atggtatagg tttcaaaacg ttcgccgttg ttgttgttga cgatggcgac
    17221 tttttcgttg ggatagatgc ctgccgcgtc caacaggtct tgatcgacgg taatgctgcc
    17281 gacgtagttc aaatcggctt cggttacggt ggcgcggtgg atttttccgc caagcatagt
    17341 acggaacatg tatttccttt tggtttgtgt gcggtctgac gggcgcgatg ccgtctggaa
    17401 atctgaatgc cgtattatag tggagtttcc ttcaaattca taatggcggc tttcggacaa
    17461 ggaaaacggt gtttcagacg gcattgcggt gtcggcttta tttgggcagg catctgtttt
    17521 gcaaggtttt taaaacgttt tttacggtgt tgattctgac ctcgatattt tccatctctg
    17581 cggtaaaccg cagcttatcg gcgccggcaa ggcggtattt tttgtcgttc tgaatcagca
    17641 ggatgatttc ggttggatcg acattattgt ttttaccgaa ggttaccgtt accgcttcgc
    17701 cggccgcatc aatggcatcg atacccaatt cttttgccat aagccgtaag tggtggcttt
    17761 cgataagggt tttgacgggt tgttcgggca ggccgaagcg gtcgacgagt tcttcgtgta
    17821 tggcgttgat ttgctgcacg gtttcgcaga cggcgaggcg tttgtagagg acgagacgct
    17881 cgtggatgtc ggggcagtag ctttcgggca gcagggcggg gctgtgcagt ttgatttcgg
    17941 tggtgatgcc cagcggtgcg tcgaggtcgg gctggcggcc ttttttgagg tcgcgcacgg
    18001 cttgtttgag catttcggtg tagagcgtga agccgacctg tatcatttcg ccggactgcc
    18061 cttcgccgag gatttcgcct gcgccgcgga tttccaaatc ctgcatggcg agggtgaaac
    18121 ctgcgccgag ttcgtctgct gccgcgatgg cgtcgaggcg tttttctgcg tctttggtga
    18181 tgtattcggg cgtgagcagg taggcgtagg cttgatggtg gctgcggccg acgcgcccgc
    18241 gaagctggtg cagttgcgcc agcccgaatt tgtcggcgcg gttgatgatg atggtgttgg
    18301 cgttggggat gtcgatgccg gtttcgatga tggtggagca gagcaacacg ttaaatcttt
    18361 gctgcaaaaa gtcgcgcatc acttgttcca gctcgcgctc gcgcagttgt ccgtgcgcca
    18421 cgccgatgcg ggcttcgggc agcagggttt ccagccgctc gcgcatattt tcaatcgtat
    18481 ctacttcatt gtgcaggaaa aacacttgtc cgccgcgttt gagttcgcgc aatacggctt
    18541 cgcgcacgct gccttcgctg aagggtttga caaaagtttt gacggcgagg cggcggctcg
    18601 gcgcggtagt aatcagcgag aagtcgcgca gaccttcgag cgccatgctg agggtgcgcg
    18661 gaatcggcgt ggcggtcatg gtgaggatat cgacattggc gcgcaggcgt ttgagctgct
    18721 ctttttggcg cacaccgaag cggtgttctt cgtcgataat cactaaacct aagtttttga
    18781 atttgatgtc gtcctgcacc agtttgtgcg tgccgataac gatgtcgacc gtgccgtccg
    18841 ccatgccttc cagcgcggct ttagtggctt tgctgttgtt gaaacgcgaa aggctagcga
    18901 ctttcacagg gaaatcggcg aaacggtcgg caaagttttg cgcgtgctgc tcgaccagaa
    18961 gcgtggtcgg ggcgagtacg gcgacctgtt tgccgcccat caccgccaca aacgcggcgc
    19021 gcagggcaac ttcggttttg ccgaagccga catcgccgca cacaaggcgg tccatcggct
    19081 tcgcctgcgt caaatcttta atcacggcgg cgatggcggc tgcctggtct tcggtttctt
    19141 cgtagccgaa gccgtcggca aacgcctgat aatccaactc gttgatttca aacttgtgtc
    19201 ccgattgggc ggcacgtcgg gcgtaaaggt taagtaactc ggcggcggta tcgcgcgcct
    19261 tttcggcggc tttgcgcttc gccttgttcc acgcgccact gccgagcttg tgcagggcaa
    19321 tgttttcatg cgcctgaccg gagtagcggc tgattaaatg cagttgcgaa acaggcacat
    19381 aaagctgcgc ttcgcctgcg tattcgagca acatcatttc gttggtttcg ccgcccaagt
    19441 ccatcgttac caagcccata taccgcccga tgccgtgttc ttcgtgaacg acggggtcgc
    19501 cgatgttgat ttcggcaagg tcgcgcaaca gaccgtctga aacggcggcg tgtttcttgc
    19561 ggcggttgtg gacgcgcgaa cgggcgacgt attggtagag atcggattcg gtgatgacgg
    19621 cgatggcact ttgagcggca actgctttgc cctctcccca accctccccc acgggggagg
    19681 gggcaggttg ctgttggatt tgcccgtgca gattactttt accggggggc aggctgcttt
    19741 cggttgaaac ggcggctgct ttcagactgt ctgaaacgac ggctgcactt tgttccctct
    19801 cctgtgggag agggctaggg agagggtttg ttgcggctgc ggaaaattcg gtttgagcgg
    19861 caactgcttt gccctctccc caaccctccc ccacggggga gggagtaggt tgctgtcgga
    19921 tttgcccgtg cagattactt gcgacgagat gcaggctgct tttggctttc aggccgtctg
    19981 aaacggcggc ggcactttgt tccctctcct gtgggagagg gctagggaga gggctaggga
    20041 gagggcttat tgcggctgcg gaaaattcag tttgagcggc aactgctttg ccctctcccc
    20101 aaccctcccc cacgggggag ggagtaggtt gctgttggat ttgcccgtgc agattacttt
    20161 taccgggggg caggcggctt tcggttgaaa cggcggctgc tttcagactg tctgaaacga
    20221 cggctgcact ttgttccctc tcctgtggga gagggctagg gagagggttt gttgcggatg
    20281 cggaaaattc ggtttggtcg gtaaccgcat cgccttctcc ttcggaggcg gaagtagttt
    20341 gctgttggtt cggcgattgc agtccgccca gtttgaaccc gtatgccaac ggcgcaactg
    20401 taatcatcag cggctcatgc gccgacaaga agccctgcca gtcggacaca ggtttggctt
    20461 tcaaaccgtt ttgctgcaag aaaccgagca tggtttcgcg ccgtcccaaa ctttcggcgc
    20521 acagcaaaat ccgtccttca aacgccgtct gaaaatcctt caatgcctgc aaaggctcgt
    20581 cggattgtcg gttgacggca aggtcgggga gggcgtgttc cttgccggaa acatcgggca
    20641 gcacttgtcc gtagtttttc agacggcctg caaaaacatc agcagagaga tacaaatgct
    20701 gtggaagcaa aggcggatag gtttcgtcgc cctgcgccat cgcgtaacgg gatttgacat
    20761 cgctccaaaa gcggtttgcc tcggcgtgaa catcgcccaa agagacaaac agcgcgtctt
    20821 cgccgatata gtcaaacagc gtttccagct cattttcaaa aaacagcggc agataatatt
    20881 ccacgcccgc gccgaagtgg ccgttgctga cggctttgta cacagccgca tcgttcggat
    20941 taccgtcgac ttcttctcgg aagcggctgc ggaagatttt ttgcgcttcg ctgtcggtgg
    21001 ggaactcgtg cgccggcagc aggcggattt cggaaacagg agaaatggtg cgttgtgttt
    21061 cggtatcgaa agttttgatg ctgtcgattt catcgtcgaa caaatcaatg cggtacggca
    21121 tttcgctgcc catcgggaac aaatcgacaa taccgccgcg cacagcgaac tcgcctgccg
    21181 cgacaacgtg ggaaacatgg ttataacctg catccaccaa atcgcttttc agacggccta
    21241 tatccaaagt ctgccctgtt ttcagccaaa acgtgcgccc cgccagaaac ggcacaggcg
    21301 gcagcttctg catcgccgtg gcaaccggca cgaacaacac atccgccgcg ccgcttttaa
    21361 tctgccacaa cgccgacagc cgctccgaca ccaaatcctg atgcggcgaa aaacgctcgt
    21421 aaggcagcgt ttcccagtcc ggcaggaaca ccgccgtgtc gtgcggacgg aaaaaccgcc
    21481 atgccgtctg aaggcgcaac gcctgttccg catcttgggt cagcacgacc ttgagccgct
    21541 tgtgcggcag ataacgcgcc aaagccaagg gcagcgagcc ttgcgaaaga ttgggccaac
    21601 gggatttttc acggggtttg gggatggggt aggtcatagt gcggcaatcg gcggacagtg
    21661 atgcaaacgg tcattttagc acgggagggg gcgcgatgcc gtctgaaagg tcggcggatt
    21721 gttgcccggc gcactttgtg gtaaagttta gaaaatcaac tctaaactgt ggcaggcggt
    21781 ttgaccggcc gccgattaat gggaaccgac agaggaagcc gatgatacac accgaaccga
    21841 gcgcgcagcc gtcaactatg gacacggctg cttttttaaa gcacatcgaa tccgcattcc
    21901 gccgcatttt ttcagacggt atcgacctga tgcgatacct gcccgaagac aaatggcttg
    21961 ccttgaagca ggcgggtttg ctgttgccct tcctcgacaa aaaacacggc gggcgcaagg
    22021 gcagccagtt tgaaattcag gaagtcttgc ggattgcggg gcattacggc gtgcccgtta
    22081 cgctgcgtac cggcatcgaa ggcgcgctgg tgttgcagcc actgcaagag ttcggcgatg
    22141 aagcgcaagt cgcgcaaggt ttggagatga ttttcaaagg cgagggcggc ggtttgggcg
    22201 ttaccgaacc cgaaacctcc ggcgcggcga ttgcacgcga aatgcagtct tactacgaat
    22261 ataccgacgg acaaaccatt tacgtcaacg ccgcgaaata ctggcagggc aactcgcaaa
    22321 gcgacttcct cctcgttgcc gccaaagagc gcaaaaacgg caaactcgcc aaagtcatcg
    22381 acctgctgct cgtccccaaa acatacatcc gctgcgaaac cctcgcatcc gaaggcttgc
    22441 gcgccgtccg ttacgccgtc aaccgcatcg atgccgaaat gcctgcaacc gccgtgatga
    22501 aactctccca gagcgacgct gccggtttgc gcgcgttcca aaacatcttt atccgcagcc
    22561 gcctgcaact gatcggcatg acgcacggca ttatggaata catccttgaa aacctgggac
    22621 gatacgtccg caacgacatc agattcgtcg attacgaacg ccgcgaaatc cggcgccgcc
    22681 atcaggtttc cgagattctt taccgctacg tctgccattc cgtttcgcct gttgcccccg
    22741 tcgcccatca gctgatggag gcgaacatcg tcaaaaccct cgccacggaa tacacttatg
    22801 ccgccgcgca aatgttgcaa aaactcttgg gcgcgaaggg ttttgaacgc ggacacaccg
    22861 ccggcaatat cgccatcgac atccgtccct tcacgatttt tgaaggcccg aacgatatgc
    22921 tttatgccga aatttacgac cagtttgtcc gcgccaccgc cgaagaaaaa gaagcaggca
    22981 tgaagttgga caaaaaccaa accctgctcg accgcctgca aaccgatgcc cgctttgccg
    23041 ccgtcgcccg cgactacact ttgcctgaag acatccgcag cttcctgcag gaacacaccc
    23101 tgaccgatgc ctgcgccctg caaaaagtct ttatcggcaa aatcatcgcc cgactctttg
    23161 tcttcgtaca ggcgaaacac gaagacgcgg cagcctgcct gctgaacgac atccgcaaag
    23221 acatattgga ctgccgatat tgcggatagc gggcaaatac ggcggcaggc aatgccgtct
    23281 gaaaggggaa gtgcctttag acggcatttt cggcggcggc aggcagattt cgggcggcgc
    23341 aaaaggcaac gcgcggcatt ctgcaaagcc gccgattgcg gcggataccg catttcgcgc
    23401 cgcgcccttg cgccgctttc ccacttaccc tatttgttcg gaacatattt atgaaaaaga
    23461 gaaaaatact gccgctggca atttgtttgg cggctttgtc tgcctgtacg gcgatggagg
    23521 cacgcccgcc ccgggcaaat gaagcccaag ccccccgcgc ggatgaaatg aaaaaagaaa
    23581 gccgccccgc gtttgacgcg gcagccgtat ttgacgcggc agccgtaccg gtatccgaca
    23641 gcgggtttgc cgccaatgca aatgtccgcc gttttgtgga cgatgaagtc gggaaagggg
    23701 atttttcccg ggcggaatgg caggattttt ttgacaaagc ggcttacaag gcggacatcg
    23761 tcaagattat gcaccgcccc tccacatcgc gtccgtggta tgtgttccgc acgggaaatt
    23821 cgggcaaggc gaaatttcgc ggcgcgcgcc ggttttatgc ggaaaaccgc gcgcttatcg
    23881 atgatgtggc gcaaaaatac ggcgtgcctg ccgaacttat cgtggcggtt atcgggattg
    23941 aaacgaatta cggcaaaaat acgggcagtt tccgtgtggc ggacgcattg gcgaccttag
    24001 gctttgatta cccccgccgc gccgggtttt tccaaaaaga attagtcgag cttttaaagc
    24061 tggcaaaaga agaaggcggc gatgttttcg cctttaaagg cagctatgcg ggcgcaatgg
    24121 ggatgccgca atttatgcct tcgagctacc ggaaatgggc ggtggattat gacggggacg
    24181 gacatcggga catatggggc aacatcggcg atgtcgcggc atcggttgcc aattatatga
    24241 agcagcacgg ttggcgcacg ggcgggaaaa tactggtgtc tgcaacattg gcgccgggtg
    24301 cggatgttca ggcaatcatt ggcgaaaaaa ccgccctgac gcggacggtg gcggatttaa
    24361 aggcgtacgg catcatcccc ggcgaagagc ttgcagatga tgaaaaggcg gttttgttca
    24421 aactggaaac cgcacccggc gtgtttgaat attatttggg cttgaacaat ttttatacgg
    24481 tatggcagta caatcacagt cggatgtatg taacggcggt cagggacatt gccaattcgc
    24541 ttggcggccc gggattgtaa tgttttttaa aatgccgtct gaaccacgtt tcggttcgga
    24601 cggcattttt gcatcctttt atggggattt cccgcatcag ggcggtattc ggggcgaatc
    24661 ccgcttcaga cggcattgcc ggtcaaggct tgtccgaagg aggatgtccc tgttctttgg
    24721 cgggcgggag gaaaagtatg cagggattgc ttttaatctg attccaaaaa cttttaatca
    24781 gattgcggat gctgccgatt ttcgtgccgc gcgagcgcag ggcgacgaag agggcgaggc
    24841 tgaagctgac gcagaggttg accaggccga tggcgaggac gctgaaaatg ccgagtacga
    24901 acgtgcccaa accgacgttg ccgctgacgg cggcatagcc gaggttggcg gaggaaaagg
    24961 cgacgtggcg gatgtccagc ggcagcccga ggaggtgtcc gaaatagccg gtcataccca
    25021 agagcatccc gaagatgaag ttgcctgcca gtgagccgta gtgtttgtgg atgtaggcgg
    25081 cgaggacgcg gcggggtccg gggcgcatta ttttacgcag caaggggttg aagggcaggc
    25141 gttggcgcag gttgaggtag tcggcgcggt tgtcgaagaa gcctgcgatg atgccggagc
    25201 agaacagcca cagacctgca atggcggcat accacagcgt cgggtaagcg atgatgtcta
    25261 tggatttgaa ctggtaggcg gcggtgtggg catcgagtat gggctgccgg tacagatggg
    25321 catagccgaa cgatatggcg cacgccaaaa ggatggcgat ggaaacgttg ccgaagacgg
    25381 cgacactttg ggagcggcat acgtcgatga ggagcttggc gagtttgttg tccaccgctt
    25441 tgccgccttc gttgaggtcg acctgttcgg caaagctggc ggcagtcatc gcgggctgtt
    25501 tggtggcgac ggtgcagtgc agcatatgga tgatcataaa gccgatgccg tagttgaacc
    25561 cagccgacaa ggaagtcagg aaggggctga ggccgagtgt gccgatgcgg attttgagca
    25621 gcgccatcag ggcgatgatg atgccgccgc ctgccgccga gtagaacatt ttgaaatatt
    25681 ctttgcggtt gcgggtgatg tagtgttcgc cgtggtggct tttgttttcg gtaatgctgc
    25741 gggcgagcat acggatgctg ctgcggcgca ggtggcgggt gctgtattgt tcgaccgccg
    25801 cgtagatgag ggagttcatc agcgcgatgg tcaggcggtt gctgttgccg gcgccggttt
    25861 ggatgtcggt gagcagcttc agacggtcta cggtctgccg gagccgttcg agcaggtggg
    25921 cgactttgac cgacgaaccg gagccggcgc ccgtcccttt gcggcgcaaa tagtcaatct
    25981 ggctgaaaca ttggtcgaac atcacttcga ggtgggcggt gtcgtaaggc gcggtgccgt
    26041 tgcggtagtg ttcgaccagt ttggcggttt cgcgttggag ggcgacgaag gaagaatcgg
    26101 cttccagcag gcgcggggcg atgcggatga ggtcgggttc gatggcttcg gatgccgtcc
    26161 agacagacag catttcgatg gcgcgcaaac gcgcatcggc aaggcggatg cccgccgtct
    26221 gcaatttgga cggttcggcg tggctccgga tgagttcgta taaaaccagc cattggcgga
    26281 tattgagcgt ttgcagccat ttgtcgtcgt tttcggaatg gaaaagataa aggaagactt
    26341 cgcccaagtt ggcaaaatct ttatatgacg ggctgaagcg ttcgtagatg cgtatgccca
    26401 tttcccgggc aaagctgtgg cgcgagaaga tgccgagttt gatgagtgcc ggataaatgt
    26461 gtatttgcgc gagccagatg taaaaacacc ggctgaagcg ggacagtaat tccctgtctt
    26521 gtttgaatgt gtcgataatc aggtcgaaac gttcccccgc acattttttg ccgccacggc
    26581 gcaaaaattt aatcagtgcg ttgaggacgt tgacaaaatc ggtatgtccc aagctttccg
    26641 aaagcagggg gcgcaggttt tgaggggtaa ttttcttcat atcagccatt gtaccgcatc
    26701 ggcaatccgg cgttaaacgc cccgatagcg gggagggcgc gcctgcggtg ggcggcgcat
    26761 tttccgccgg tttgaaatca aactatatta atgatataat aaatatcgat aaatcaatct
    26821 gatagaataa tccgtattga tttttaattt tttcgattat aacaatttcg gccggtttgt
    26881 gcatttcgtc atccggaagc cggtatttta ccggcaggtt ttgaccgctt tgcccgatga
    26941 ccgggtttgt ctgtttttaa caaaggagtg agaaggtttg tccctgtctg aatttataga
    27001 acgccgaacg tcatttaatc cgatggttat tttgacgact ttgttttttg tgtgtgtttt
    27061 ggtggtattg gttttaaccg tgccggatca ggtgcagatg tggctcgatc gggcaaaaga
    27121 agtcattttt accgagttca gctggtttta tgttttaacg ttttccattt ttctgggttt
    27181 cctgctgata ctctcggtca gcagtttggg aaacatcagg ctcggacggg atgaagatgt
    27241 gccggaattc ggcttcctgt cgtggctggc gatgctgttt gcggccggga tgggcgtggg
    27301 tctgatgttt ttcggcgtgg cagagccgtt gatgcattat ttttcggaca ttacggccgg
    27361 cacgccggaa cacaggcagc agcaggcatt gctgcacacg gtgttccatt ggggcgttca
    27421 cgcttggtcg gtgtacggta cgattgcatt ggctttggct tatttcggtt tccgctacaa
    27481 gctgccgctt gccctgcgtt cttgttttta ccccctgttg aaagaaaaaa tttccggaag
    27541 gttcggcgat gccattgata ttatggcgtt gcttgctact tttttcggca tcatcaccac
    27601 attggggttc ggggcttcgc aactgggcgc cggattgcag gaaatgggct ggattgccga
    27661 aaacagcttc agcgtgcagg ttttgattat cgccgccgtc atgtccctcg ccgtcgtttc
    27721 ggcaatatcc ggcgtgggga agggcgtgaa ggtgttgagc gagttgaacc tgggccttgc
    27781 gtttttgctg ctgttttttg ttttggcggc gggacccact gtttacctgt tgtcggcatt
    27841 cggcgacaac atagggaact acctcggaaa tctggtgcgc ctcagtttta aaacttatgc
    27901 gtacgaacgg gaacacaagc cgtggtttga atcttggacg gtgctttatt gggcgtggtg
    27961 gtgttcttgg gcgccgtttg tgggtttgtt tatcgcgcgc atttcaaagg ggcgcaccat
    28021 ccgcgagttt gtcttcgggg ttttgctcat ccccggcctg ttcggcgttt tgtggtttac
    28081 cgtcttcggc aatacggcga tttggctgaa tgacggggtt gcggggggaa tgctcgaaaa
    28141 gatgacctcc tctccggaaa cgctgctttt taaattcttt aattacctcc ccctgcccga
    28201 attgacgagc atcgtcagcc tgctggtcat ttctctgttt tttgtaactt ctgccgattc
    28261 cgggatttat gtcctgaaca atattacctc tcgggacaaa ggcttgagcg cgccacggtg
    28321 gcaggcggtt atgtggggcg tgctgatgtc tgccgttgcc gttttgctga tgcgctcggg
    28381 cggactcggc aacctgcagt ctatgaccct gattgtttcc ctgccgtttg ccctgctgat
    28441 gctgataatg tgtttcagcc tgtggaaagg cttgagtgcg gataagaaat attttgagac
    28501 ccgggttaac cctaccagtg tattttggac gggcggcaag tggaaagaac ggctggtgca
    28561 gataatgagc cagacgcagg agcaggatat tttaaaattc ctcaaacata ccgcatcgcc
    28621 cgctatgcac gagttgcaac gggagctttc ggaagaatac ggcttgagcg tccgggtcga
    28681 taagatgttt catcaggacg agcccgcaat cgagtttgtc attcgcaaag agacgatgcg
    28741 cgattttatg tacgggatta agtctgtcgg gcaggatgta tccgatcagt tgattaacga
    28801 cggcaacttg ccgcatatcc ggcaccagac aacttacaaa ccttacgctt attttttcga
    28861 cgggcgcgtc gggtacgatg tgcagtatat gaacaaggac gagctgattg ccgacatttt
    28921 gaaaaactac gaacgttatt tgatgttgtt ggatgatgtc ggtcaggaac tgatggcgca
    28981 cgagcaggtg gaattggcag agtaagtgcc ttatctccgg cgtttgccaa aaaaaatgcg
    29041 gtgaatcaga ttcaccgcat ttttttgccg gacggggctt cggacggcac ggcgttcatt
    29101 tgtttccgtc aaactgctcc ttcaacaccc gtttcaatac cttgcccgta gcgttgcgcg
    29161 gcagcccgtc tttaaagtgg atttgtttgg ggattttgaa atttgccagc acggtacgca
    29221 ggtggcggcg gatttcgttc tcgcccaaat ccataccttc cttcaattgg acgaaggcga
    29281 cgatttcctc gtcggcataa cggtctttca cgccgatgac ggcagcggct tcgacggcat
    29341 cgagtttgta gatttcttct tcgatttcgc gcggatagac gttttgacct ttggaaataa
    29401 tcaaatcttt tttgcggtcg acgataaaga taaagccgtc ttcgtctatg gtaacgaaat
    29461 cgcccgtttt caaccagccg ttgacgatgg tttcatcggt ggcggcaggc atattgaggt
    29521 agccccgcat caccgaaccg cccctgacga tcagttcgcc cacttcgccg cgcggcactt
    29581 cgaccaattc ttcatcgacg gctttggctt ccaaaccggg cagggggatg ccgacgctgc
    29641 gggctttttg cctctcgggc gtattgacgg cgacgacggg agaggcttcg ctcagtccgt
    29701 agccttccag caatttggcg cgggggaact tggctttgaa atcgaggatg gtttgttccg
    29761 ccaaaggcgc accgccgctg ataaacaggc gaatgcggtt gaaccatctg aaataccaag
    29821 ggattttcgc cttgctcatt gcggtgtaaa tcgcgggtat gcccaaaaac acggtcgcgc
    29881 gtttgagcag ggcctgtttc aaaacgttgg aaaacgggaa aacggatttg accaaaataa
    29941 tcgaacacgc catataaatc ggcagcagca ccatagccgt cagcgtgaag ctgtggaaca
    30001 tcggcaggaa aacgataaag cggtcgcgct tggagatttt aaagatgcgt tcgatgccgt
    30061 tcaggttggc gaacaggttg gcgtaactga ttagcgcgcc tttgggatgc cccgtcgtgc
    30121 cggaggtgta gatgatgtgt gccaaatcat ttatccgggg ttggcggccc aagtcgggtt
    30181 tttcggggaa gcggcgcacg tcttcaaaaa aggcatcgcc ttccgccatt tcgccagccg
    30241 gacggctttt gtccgtccaa atgatttttt cgacgggcgt ttgcgccttc aagcccgcca
    30301 actcttttga caggcccgcc gaggcgaaca ggaagcgcgc cttgcagtcg ttcaggatat
    30361 acgcgtattc gctgtttttc aaaaatgtgt tcatcggtac ggcgaccgcg ccgatggcgg
    30421 agacggcgaa ataggcggta ataaattctg tggaattgga aaccgccagc gcgaccgtgt
    30481 cgccgaactt cacgccgata ttttgcagat acgccgcgac ggcttcggcc tcctgcttga
    30541 gcgcgcggta ggcggttttt tccttgccgt cgaacactgc cgtgccgttg ccgtttttgc
    30601 ggcaggcggc ggcgagcatt tcgtagaaat tggcataagt ccggttcatg aaacttcctt
    30661 tatgg
//
LOCUS       DAWHRA010000004        28824 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWHRA010000004 DAWHRA010000000
VERSION     DAWHRA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28824
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.004"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            183..539
                     /locus_tag="WIB30_000141"
     CDS             183..539
                     /locus_tag="WIB30_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ3796286.1"
                     /translation="MLSAYFPVFVFILIGLAAGVLFILLGTILGSKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKELGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            530..1012
                     /locus_tag="WIB30_000142"
     CDS             530..1012
                     /locus_tag="WIB30_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ3796287.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1025..1618
                     /locus_tag="WIB30_000143"
     CDS             1025..1618
                     /locus_tag="WIB30_000143"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ3796288.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYVSVMTT
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVQNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1608..2864
                     /gene="nuoD"
                     /locus_tag="WIB30_000144"
     CDS             1608..2864
                     /gene="nuoD"
                     /locus_tag="WIB30_000144"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ3796289.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVTPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2864..3337
                     /gene="nuoE"
                     /locus_tag="WIB30_000145"
     CDS             2864..3337
                     /gene="nuoE"
                     /locus_tag="WIB30_000145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ3796290.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFIADYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3727..5028
                     /gene="nuoF"
                     /locus_tag="WIB30_000146"
     CDS             3727..5028
                     /gene="nuoF"
                     /locus_tag="WIB30_000146"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ3796291.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5028..5465
                     /locus_tag="WIB30_000147"
     CDS             5028..5465
                     /locus_tag="WIB30_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796292.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRGNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            5709..5990
                     /locus_tag="WIB30_000148"
     CDS             5709..5990
                     /locus_tag="WIB30_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ3796293.1"
                     /translation="MRNHPLTRQPAVYILANGKNGTLYIGVTMNLPERVWQHKNHVNI
                     DGFTARYDVHDLVWYQFFENMPEAVAKEKTMKKWRREWKIKLIEEQNTE"
     gene            6302..8563
                     /locus_tag="WIB30_000149"
     CDS             6302..8563
                     /locus_tag="WIB30_000149"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ3796294.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGSLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAN
                     GLGVKNFATRLRQQDKRLSDDLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELAAATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLSSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVMHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8566..9642
                     /gene="nuoH"
                     /locus_tag="WIB30_000150"
     CDS             8566..9642
                     /gene="nuoH"
                     /locus_tag="WIB30_000150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ3796295.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFAKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGLWMISPLNLWK"
     gene            9724..10203
                     /gene="nuoI"
                     /locus_tag="WIB30_000151"
     CDS             9724..10203
                     /gene="nuoI"
                     /locus_tag="WIB30_000151"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ3796296.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            10234..10941
                     /locus_tag="WIB30_000152"
     CDS             10234..10941
                     /locus_tag="WIB30_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796297.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            10977..11648
                     /locus_tag="WIB30_000153"
     CDS             10977..11648
                     /locus_tag="WIB30_000153"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ3796298.1"
                     /translation="MTFQLILFYIFAVIILYGALKTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPTDYNNIRDLGSRIYTEYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGFKPE
                     GEGKA"
     gene            11645..11950
                     /gene="nuoK"
                     /locus_tag="WIB30_000154"
     CDS             11645..11950
                     /gene="nuoK"
                     /locus_tag="WIB30_000154"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ3796299.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDGLKG"
     gene            11984..12559
                     /locus_tag="WIB30_000155"
     CDS             11984..12559
                     /locus_tag="WIB30_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ3796300.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            12683..13024
                     /locus_tag="WIB30_000156"
     CDS             12683..13024
                     /locus_tag="WIB30_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796301.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            13073..15097
                     /gene="nuoL"
                     /locus_tag="WIB30_000157"
     CDS             13073..15097
                     /gene="nuoL"
                     /locus_tag="WIB30_000157"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ3796302.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIVSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYLAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPVYVLFENKYYLDVLYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            15193..16689
                     /locus_tag="WIB30_000158"
     CDS             15193..16689
                     /locus_tag="WIB30_000158"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ3796303.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGSDSRAPFARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLIMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIEQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     QDINCREFAILAILAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            16699..18141
                     /gene="nuoN"
                     /locus_tag="WIB30_000159"
     CDS             16699..18141
                     /gene="nuoN"
                     /locus_tag="WIB30_000159"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ3796304.1"
                     /translation="MNWSDLNLMPAMPEIVLLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFTFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMFAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVIMSLIGAFYYLRVVKVIYFDVPDHDQPVGSNYAAKFV
                     LTVNAFLLLLWGIMPQTVIDWCAKALENTL"
     gene            18253..18552
                     /locus_tag="WIB30_000160"
     CDS             18253..18552
                     /locus_tag="WIB30_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ3796305.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAVLAYILESRAGSVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(18661..19557)
                     /locus_tag="WIB30_000161"
     CDS             complement(18661..19557)
                     /locus_tag="WIB30_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ3796306.1"
                     /translation="MNPTNDLKTWQQRAQAQTELLLERFLPSEGEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSRGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGATACPDLSDAELSVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(19541..19765)
                     /locus_tag="WIB30_000162"
     CDS             complement(19541..19765)
                     /locus_tag="WIB30_000162"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ3796307.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDTDGLKELNLESDE"
     gene            complement(20012..20935)
                     /gene="rsgA"
                     /locus_tag="WIB30_000163"
     CDS             complement(20012..20935)
                     /gene="rsgA"
                     /locus_tag="WIB30_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ3796308.1"
                     /translation="MPSEHPFSDGIPTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            complement(20940..22796)
                     /locus_tag="WIB30_000164"
     CDS             complement(20940..22796)
                     /locus_tag="WIB30_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3796309.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAGATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(22822..22959)
                     /locus_tag="WIB30_000165"
     CDS             complement(22822..22959)
                     /locus_tag="WIB30_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796310.1"
                     /translation="MPSEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(22959..23543)
                     /gene="ruvA"
                     /locus_tag="WIB30_000166"
     CDS             complement(22959..23543)
                     /gene="ruvA"
                     /locus_tag="WIB30_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ3796311.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHAVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(23625..23951)
                     /locus_tag="WIB30_000167"
     CDS             complement(23625..23951)
                     /locus_tag="WIB30_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ3796312.1"
                     /translation="MNIKHLLLTSAATALLSISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(24013..24732)
                     /locus_tag="WIB30_000168"
     CDS             complement(24013..24732)
                     /locus_tag="WIB30_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ3796313.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFTSQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(24729..24902)
                     /locus_tag="WIB30_000169"
     CDS             complement(24729..24902)
                     /locus_tag="WIB30_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ3796314.1"
                     /translation="MKRGGNTVTQAAFERRFMPSENLSGHIPNRRPHCTAVRNVSGGH
                     DAHSSSTQERNTF"
     gene            complement(24862..25326)
                     /gene="trmL"
                     /locus_tag="WIB30_000170"
     CDS             complement(24862..25326)
                     /gene="trmL"
                     /locus_tag="WIB30_000170"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ3796315.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(25391..25999)
                     /locus_tag="WIB30_000171"
     CDS             complement(25391..25999)
                     /locus_tag="WIB30_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ3796316.1"
                     /translation="MAAYRTDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHY
                     EPPVSNMIRALKHLADLGMVQPLADLMMQNPPDLLADECFDFVLPVPLSRERLLQRGF
                     NQSEGIVGLLAQRYGWRILPRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPPPKNC
                     NILLIDDVFTTGATLDELAKTLKKSGANRICCWTLARTPMKK"
     gene            25983..26858
                     /gene="bioH"
                     /locus_tag="WIB30_000172"
     CDS             25983..26858
                     /gene="bioH"
                     /locus_tag="WIB30_000172"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ3796317.1"
                     /translation="MRYAAKSLRQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKS
                     MPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDAPFAQPFDIEAAAD
                     AVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTADEDYPEGLAAP
                     ALGKMVGAFRTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARCGTPSALQEALDAA
                     ERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRRLKGSRLVVMEKAAHAPFLSH
                     AEAFAALYRDFVEGV"
     gene            26860..27648
                     /locus_tag="WIB30_000173"
     CDS             26860..27648
                     /locus_tag="WIB30_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ3796318.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            27869..28106
                     /locus_tag="WIB30_000174"
                     /pseudo
     CDS             27869..28106
                     /locus_tag="WIB30_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28437..>28824
                     /locus_tag="WIB30_000175"
     CDS             28437..>28824
                     /locus_tag="WIB30_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HEZ3796319.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQISEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTST"
BASE COUNT         6493 a         7126 c         7920 g         7285 t
ORIGIN      
        1 acgaattacg gtgtaaaaat ttatatgacc ttataaaatc aaataagaat cgttatcata
       61 acatgattgt atttattggg tttttttggg cgttttgccg atatttacct tttaatggtt
      121 tttgaaattc gctaaaatac gaaattattg tagaaatttt gttaacggat ttgggtgtaa
      181 ccatgttgtc cgcttacttt cccgtctttg tctttatcct catcggcctc gcggccggcg
      241 tactgtttat cctgctcggc acaattttgg gctcgaaacg tcactatgcc gaaaaagacg
      301 caccttacga atgcggtttt gaagcctttg aaaacgcaag gatgaagttc gacgtgcgct
      361 attacctcgt cgccatcctc tttatcttgt ttgatttgga ggtcgcgttc atgttgccgt
      421 gggcagtcgt gttcaaagaa ttgggcgcat acggcttctg gtctatgctg gtgtttatcg
      481 tcgttttgac ggtaggcttt gtttacgaat ggaaaaaagg tgcgctggaa tgggaataga
      541 aggcgttttg aaaaaaggtt tcatcaccac cagcgcggat acggtgctga actatatgcg
      601 taccggttca ttgtggccgg ttactttcgg cttggcctgc tgcgccgtgg aaatgatgca
      661 tgcaggtatg gcgcgttatg accttgaccg tttcggcatc attttccgtc cgtccccacg
      721 tcaggccgac ctgatgattg tggcaggtac gctgaccaac aaaatggcgc cagccctgcg
      781 ccgcgtgtac gaccagctcg ccgagccgcg ctgggtattg tctatgggct cgtgtgccaa
      841 cggcggcggc tattatcact attcttattc cgttgtgcgc ggtgctgacc gcgtcgtgcc
      901 ggtagacgtt tatgtgccgg gttgtccgcc gactgcggaa gccctgattt acggcctgat
      961 tcagctccaa caaaaaatca agcgcacttc caccatcgcg cgtgacgagt aaggagagga
     1021 cgatatggca agcattcaaa acttatacga gaccgtcgtc ggcgtgcttg gcgatcaggc
     1081 aagcaaagtc atttcagctt tgggcgagat taccgtcgag tgtctgcctg aacactatgt
     1141 ttcagtgatg actacgctgc gcgaccatga agaactgcat ttcgagcttc tggtcgactt
     1201 gtgcggtgtc gattacagca cttacaaaaa cgaagtatgg cagggcaaac gctttgccgt
     1261 cgtcagtcag ctgctttctg ttcaaaacaa tcaacgcatc cgcgtacgcg tctgggtttc
     1321 agacgacgac ttccccgtag tcgaatccgt cgtcgatatt tacaacagcg cggattggta
     1381 cgaacgcgaa gccttcgata tgtacggcat tatgttcaac aaccacccgg acttgcgccg
     1441 catcctgacc gattacggct ttgtcggaca tccgttccgc aaagacttcc cgatttccgg
     1501 ttatgtggaa atgcgttacg acgaagagca aaaacgcgtg atttaccaac ctgttaccat
     1561 tgagccgcgc gaaatcacgc cgcgtatcgt ccgtgaggag aactacggtg gccaataaat
     1621 taagaaacta caccatcaac ttcggcccgc aacaccctgc ggcgcacggc gtattgcgta
     1681 tgattttgga gttggagggc gaaaccatcg tccgtgccga cccgcatatc ggccttctgc
     1741 accgaggcac agagaaactg gctgaaacca aaacctacct gcaagccctg ccctatatgg
     1801 accgtttgga ctacgtttcc atgatggtca acgagcaggc gtattgcttg gcggtagaaa
     1861 aacttgccgg tatcgatgtg cctatccgcg cccaatacat ccgcgtgatg tttgccgaag
     1921 taacgcgcat cctcaatcac ttgatgggta tcggttcgca cgccttcgac atcggcgcga
     1981 tgaccgccat cctttacgcc ttccgcgacc gcgaagagct gatggatttg tacgaagccg
     2041 tgtccggcgc gcgtatgcac gccgcctact tccgtcctgg cggcgtttac cgcgacctgc
     2101 ccgacttcat gcccaaatat gagagcagca aattccgcaa cgccaaagta ttgaagcagc
     2161 tcaacgaatc ccgcgaaggc accatgctcg actttatcga cgccttctgc gaacgtttcc
     2221 cgaaaaacat cgatacactc gaaaccctcc tgactgacaa ccgtatttgg aaacagcgta
     2281 ccgtcggcat cggcgttgtc actcctgagc gcgccatgca aaaaggcttt accggcgtta
     2341 tgttgcgcgg ttcaggcgtg gaatgggacg tgcgtaagac acagccttat gaagtgtacg
     2401 acaaaatgga tttcgacatc cccgtcggcg tcaacggcga ctgctacgac cgttacctct
     2461 gccgtatgga agaaatgcgc caatccgtac gcatcatcaa acaatgttcc gagtggttgc
     2521 gtgtcaatcc gggtccggtc attaccacaa accacaaatt cgctccgccc aaacgtaccg
     2581 aaatgaaaac aggtatggaa gacctgattc accatttcaa actctttacc gagggtatgc
     2641 acgttcccga gggcgagacc tacaccgctg tcgaacatcc gaaaggcgag ttcggcgttt
     2701 acatcatttc agacggcgca aacaaaccct accgcctgaa aatccgcgca cccggcttcg
     2761 cccatctgca aggcatggac gaaatggcaa aaggccacat gctcgccgac gtcgttgcca
     2821 tcatcggtac gcaggacatc gtattcgggg aggttgaccg ataatgttat ccgcagaatc
     2881 cttaaaacaa atcgacatcg agttggcgaa atatcccgcc gaccaacgcc gctctgcgat
     2941 tatgggcgca ttgcgtattg cccagaccga aaaaggctgg ctcgcccccg aaaccatcgc
     3001 ttttatcgcc gactacatcg gcatctcgcc tgcacaagcc tacgaagtcg ctaccttcta
     3061 caatatgtac gaccttgagc ctgtcggcaa atacaaactg accgtttgta ccaacctgcc
     3121 ctgcgcccta cgcggcggta tggcgaccgg cgaatacctc aaacaaagac tcggtatcgg
     3181 ctacggcgaa accacgcccg acggcaaatt tacccttgtc gaaggcgaat gcatgggcgc
     3241 gtgcggcgac gctccggtta tgctggtcaa caaccacagc atgtgcagct ttatgaccga
     3301 agaggcgatt gagaagaaac tggcggagtt ggagtaggtc gtctgaaacg acgatttaaa
     3361 cgtaggtcgg atacttgtat ccgacagagt gggtaaaaag gcaaaatgtc ggatttaaga
     3421 atccgcccta ctgaaatacc gaaatgccgt cattcccgcg caggcgggaa tccaccggta
     3481 agattcggtt tctgaattta ataagacatt gcttaccatt gaggatggat tcccgcctgc
     3541 gcgggaatga cgacagacaa gcaagtggtc gagatccaac aaaaacgatt aaaggtcgtc
     3601 tgaaaatatc gatttgataa actagatttt atttcagacg acgttacaag ccggtacaca
     3661 aagacatctt aaggtcgtct gaaacagcgg ccgcaaccga tacgaaaaca aacaggcaca
     3721 ccaaaaatgg ctatttacca atcaggcgtg atttttgacc aagtggatac cgccaatccc
     3781 gactgctgga cattggacga atacgtcaaa cgcggcggct ataccgccct gcgtaaaatt
     3841 ctgtccgaaa acatctcgca aaccgatgtg attgacgaag tcaaaacctc cggcttgcgc
     3901 gggcgcggcg gtgcgggctt cccgactggt ttgaaatgga gctttatgcc ccgttccttc
     3961 ccgggcgaaa aatatgtggt ttgtaacacc gacgaaggcg aaccgggtac atttaaagac
     4021 cgtgacatca tcatgttcaa tcctcatgcc ctgattgaag gcatgattat cgccggttac
     4081 gcgatgggtg cgaaagccgg ctacaactat atccacggcg aaatcttcga aggctaccaa
     4141 cgctttgaag ccgctttgga gcaggcgcgt gccgcaggct ttttgggtaa aaatattttg
     4201 ggttcggatt ttgaatttga actcttcgcc caccacggct acggcgcata tatttgcggc
     4261 gaagaaaccg cattgctcga atcgttggaa ggcaaaaaag gccagccgcg cttcaaaccg
     4321 ccattccctg cttcgttcgg cctgtacggc aaaccgacca ccattaataa tactgaaacc
     4381 ttttcctccg tcccattcat tatccgtgac ggcggacagg catttgccga taaaggtatt
     4441 ccgaatgcag gcggtaccaa attattctgt atttccggcc atgtcgagcg tccgggcaac
     4501 tatgaagtgc cattgggtac gccgtttgcc gaagtcttga aaatggcggg cggtatgcgc
     4561 ggcggtaaaa aactcaaagc cgtcattccc ggcggttcgt ccgcgcccgt attgcctgcc
     4621 gacatcatga tgcagaccaa tatggactac gactcgatct ccaaagcagg ctccatgctc
     4681 ggttccggcg cgattatcgt catggacgaa gacgtgtgca tggtcaaagc ccttgagcgt
     4741 ttgagctact tctactacga cgagtcttgc ggccaatgta ccccctgccg agaaggtacg
     4801 ggctggcttt accgcatcgt ccaccgcatc gtagaaggca aaggtaaaat ggaagatttg
     4861 gatttgctgg attccgtcgg caaccaaatg gcaggccgca ccatctgcgc cctcgccgat
     4921 gctgccgtct tccccgtccg cagctttacc aagcatttcc gtgatgagtt tgtgcattac
     4981 atcgaacacg gcgggccgat gaaagagcat aagtggggag ggtggtaatg gtggaagcta
     5041 aaatttttat tctatacggt gcagccaaca aaggtaagag tacgacactc aatacgcttt
     5101 ttaatcagat ttgtcggaaa ttttctaaat ttctagtctt ttttgaaaga catggaaacg
     5161 gcttagattt tgttgcagta tttgatcatg aaggtcagag aattggtttt tattcatctg
     5221 gtgataatga atacgaggtt aggggaaatt tatacaaact ttattcgcat aattgtgatt
     5281 ttatttttgg cacgtcaagg acacggggtg gtagttgcga tgcagtagga tgttatgcag
     5341 agttattgca tggcgatgta aatataatta attggtgtga aaagtttgag cctacagatg
     5401 aagacaatga gcgtgctgtt aaagagttat ttaagtcatt taaaaatata ataaatgagt
     5461 tatagtttta gttggtttta tattggttaa aagcaaaatg ctaaaaattt aactttgccg
     5521 tcattcccgc gtaggcggga atccatagtg gaatttacag aacccgatat ttgaaaagca
     5581 gttgccgaaa ttcaaaaaat ggattcccgc ctacgcggga atgacggcgg gagtaggcag
     5641 atgttttcag atgaaaacgg ttgtaaatga tattaaaaaa gttgttgttt atattgcagg
     5701 aaaaatgaat acgaaaccat ccgcttacta gacaacctgc cgtatatatt ttggcaaacg
     5761 gtaaaaatgg aacactctat atcggtgtta ccatgaattt gccggaaagg gtttggcagc
     5821 acaaaaacca tgtcaatatt gatggcttta ctgcccgata tgatgtgcat gatttagttt
     5881 ggtatcagtt ttttgagaat atgcctgaag cagttgccaa agaaaaaacg atgaaaaaat
     5941 ggcgacgtga atggaagatt aaactgattg aagaacaaaa tactgaatga ttggacttgt
     6001 cgggcgtgtt gtttgtttag ttttatttct ggaactttaa aaactgtcgt tattccagcc
     6061 ccacctacgc gcagacaggc tacggcggga atcaccgcaa aagttaagaa accaatgttt
     6121 gaaaacagtt accgaaaacc caagaatgga ttcacgcctg tgcgggaatg acggcaaggt
     6181 ggcagtaaac gttttaaaca gtattgattg tcaatgaaac tcaaaaggcc gtctgaaacc
     6241 catttttcag acgacctcca taaaagatta tttatcaaat acccgtaact aggaacgaac
     6301 catgttacaa atcgaaatcg acggcaaaca agtatctgtg gagcagggcg cgacggtgat
     6361 tgaagccgcg cacaagctcg gtacttatat tccgcatttc tgttaccaca aaaaactttc
     6421 catcgccgcc aactgccgta tgtgtctggt gaacgtagaa aaagccccaa aacccctgcc
     6481 tgcctgtgcc acgccggtta cagacggcat gattgtgcgt acgcattcgg caaaagcccg
     6541 agaggcgcag gaaggcgtga tggagttcct gctcatcaac catccgcttg attgtccgac
     6601 ctgcgaccaa ggcggcgaat gccagttgca ggatttggcg gtgggctacg gcaaaaccac
     6661 cagccgctac accgaagaaa aacgttccgt cgtcggcaaa gatatggggt ccttggtttc
     6721 cgccgaggaa atgagccgct gtatccactg cacccgctgc gttcgtttca ctgaagaaat
     6781 cgccggtttg caagaaattg cgatggtgaa tcgcggcgaa cactccgaaa tcatgccctt
     6841 tatcggcaaa acggtggaaa ccgagctgtc gggcaacgtc atcgatttgt gtcctgtcgg
     6901 cgcattgacc agcaaaccgt tccgcttcaa cgcgcgtact tgggaattga accgccgcaa
     6961 atccgtttcc gcccatgacg ctttgggcag caacctgatt atacaaacca aagaccatac
     7021 cgtccgccgc gtgttgccgc tggaaaacga agcgattaac gaatgttggc tgtctgaccg
     7081 cgaccgtttc gcctacgaag gcctgtatca cgaaagccgt ctgaaaaacc cgaaaatcaa
     7141 acagggcggc gagtggatgg acgtggattg gaaaaccgcg ttggaatatg tccgcagcgc
     7201 gattgaatgt atcgccaaag acggcaacca aaaccaagtc ggcatttggg ccaatccgat
     7261 gaatacggtt gaagagctgt atctggccaa aaagcttgcc aatggtttgg gtgttaaaaa
     7321 ctttgccacc cgtttgcgcc aacaagacaa acgtctttca gacgacctta aaggtgcaca
     7381 atggttggga caaagcattg aatctttggc tgacaacgat gccgtattgg tagtcggtgc
     7441 gaacttgcgc aaagaacagc cgctcctgac cgcccgcctg cgccgcgccg ccaaagaccg
     7501 tatggccttg agcgtattgg ccggcagcaa ggaagagttg ttgatgccgc ttctctctca
     7561 agaagccgca catcccgacg agtgggcagg ccgtctgaaa aacctgtctg ccaatgcgga
     7621 acacgccgtt accgccagcc tgaaaaatgc tgaaaaagca gcggtgattt tgggcgcgga
     7681 agtgcaaaac catcctgatt acgccgccat ttatgctgcc gcgcaagagc tggcagccgc
     7741 gaccggcgca gtgctgggca ttttgccgca agctgccaac agcgttggtg cggatgtctt
     7801 aaatgtaaac tctggtgaga gcgttgccga aatggcaaat gcgccgaaac aggcagtctt
     7861 gctgctcaac gtcgagcctg aaatcgatac ggttgacggt gcaaaagccg tagccgcatt
     7921 gaaacaggcg aaaagcgtga tggcatttac gccgtttgtc agtgaaacgc tgctggacgt
     7981 gtgcgacgta ttgttgccga ttgcgccgtt taccgaaaca tcaggcagct tcatcaatat
     8041 ggaaggccgt ctgcaatctt tccacggcgt ggtacaaggc ttcggcgact cgcgtccgat
     8101 gtggaaagtg ttgcgcgtat tgggcaatct gtttgacctg aaaggttttg aataccacga
     8161 taccgctgcg attttgaaag acgcgctgga tgcagaaagc ctgtcgtcca aactggacaa
     8221 ccgcagtaca tggacagggg agggcgttca gacggcatca gaccgcctcg tccgtgtcgg
     8281 cggcgtcggt atttatcaca ccgatgccat cgtacgccgt tccgcaccgt tgcaagaaac
     8341 cagccatgcc gccgtgcctg ccgcgcgtgt aaatccgaac acattggcac gcttgggcct
     8401 gcaagacgga caaaccgctg tcgccaaaca aaacggcgca agcgtatcgg ttgctgtcaa
     8461 agccgatgcc ggtttgcctg aaaacgtgat gcatctgccg ctgcataccg aaaatgccgc
     8521 gctgggtgcg ttgatggaca ctattgaact ggcgggagct tgatcatgca agaatggttc
     8581 caaaacctct ttgccgcaac gctcggtctg ggcgatttgg gcatcaccgt aggcttggtg
     8641 gtatccgtca tcgtcaaaat cgtgattatc ctgattccgc tgattctgac cgtcgcctac
     8701 ctgacttatt tcgaacgtaa agtcatcggc ttcatgcagc ttcgcgtcgg cccgaacgta
     8761 accggcccgt ggggcctgat ccagccgttt gccgacgtgt tcaaactctt gtttaaagaa
     8821 gtaacccgtc cgaagctgtc aaacaaagcc ctgttctaca ttggcccgat tatgtcgctc
     8881 gccccgtctt ttgcggcatg ggcagtgatt ccgttcaacg aagaatgggt gctgaccaat
     8941 atcaatatcg gccttttgta tatcctgatg attacctcgc tgtcggttta cggcgtgatc
     9001 atcgcgggct gggcttccaa ctccaaatat tcgttcttgg gcgcaatgcg tgcttccgcg
     9061 caaagcattt cctacgaaat cgccatgagt gccgcgctgg tgtgcgtcgt gatggtgtcg
     9121 ggcagcatga acttctccga catcgttgcc gcgcaggcaa aaggcatcgc aggcggttcg
     9181 gtattctctt ggaactggct gccgctcttc cccatcttca tcgtctatct gatttccgcc
     9241 gttgccgaaa ccaaccgcgc accgtttgac gtggcagagg gcgagtctga aatcgttgcc
     9301 ggtcaccacg tcgaatattc cggcttcgca ttcgcgctgt tcttccttgc cgaatacatt
     9361 ttcatgattc tgattgccgc gctgacatcg ttgatgttcc tcggcggctg gctgtctccc
     9421 ttcccgcaaa gctggggcat tgtcggtacg ccttccgcat tttggatgtt cgcgaaaatg
     9481 gcggcggttc tgtactggta tctgtggatc cgtgcaacct tcccgcgcta ccgttacgac
     9541 caaatcatgc gtttgggctg gaaagtgctg attcccatcg gcttcgccta catcgtagtt
     9601 ttgggtctgt ggatgatttc gccgctgaat ttgtggaaat aagtttcaga cggcatcttg
     9661 aggccgtctg aacaaagcga ttttgaatac ctaacgaaat ccctgttttg agggaacata
     9721 aatatggcta acttagtaaa aacctttctg cttggcgaat tggtaaaagg tatgggcgta
     9781 acgctcaaaa acttttttgc ccgcaaagac acaatttatt tccccgaaga gaaaacgccg
     9841 caatccgtgc gtttccgcgg tctgcacgcg cagcggcggt atccgaacgg cgaggaacgc
     9901 tgcatcgcgt gtaaattgtg cgaggcagtt tgtccggcaa tggcgatcaa tatcgaatcg
     9961 gaagaacgcg aagacggcac ccgccgcacc aaacgttacg acatcgacct gaccaagtgc
    10021 atcttctgcg gtttctgcga agaggcctgc ccgaccgatg caattgtaga aacccatatt
    10081 tttgaatacc acggcgagaa aaaaggcgac ttgcacatga ccaagccgat tcttttggcc
    10141 attggcgata aatacgaagc tgaaatagcc aaacgcaaag ccgctgacgc gccgtatcgt
    10201 taatgctttg gggcttcttg gaaggtttta aatatggaag gactgattaa tgcattgaaa
    10261 tatttagccg aacatgagcc aatagataat tttgaagaaa ttagaactag aaatagtccg
    10321 attgagttgc caagtggatt aagtaatttt gaacaaaata tttttttaaa agaaaattta
    10381 tccccaaaat tacaaaatga tgatagcttg aagacgcatt attggattat ccgtgaatgg
    10441 ggtgggatta aaagttttaa acaatctgct gaaaatagcc agcttattcg tcaattttta
    10501 tcggaactta attcgggaaa attgagtagt ggtttgttga aaatttcatc attatctaaa
    10561 ttggcttctt ttatagattg tgagcgattc gccatttatg attcacgcgc tattttttcg
    10621 ttgaattggt tgttgtttaa atttacaaat gcagatttgt tttttcagcc acaaggtaga
    10681 aatagggaac tagaaatccg aaatatgaac gtattgtttc atttttctga tatcaaaccg
    10741 aattatcgga aaccagacgt ttcgtttcat caatattgtg gattgttaca agatttggcg
    10801 aaacaagttt atggtaaaca agcaaaaccg tatcacatag aaatgttgtt attcaaaatt
    10861 gcgacaacgt ggatttgtgc ggatatggat caactgatta agtttgattg tttgcgtaac
    10921 caggattttc agactgcttg aaaccatatt tttgattaat aaagaaagca tagactatga
    10981 ctttccaact gattttattt tatatttttg cagtgataat tctttatggc gcgctcaaaa
    11041 ccgttaccgc caaaaacccc gtgcatgccg ctttgcatct ggtgctgacc ttctgcgtga
    11101 gcgcgatgat ttggatgctg atgcaggcgg agtttttggg cgttacgctg gtggtcgtgt
    11161 atgtcggcgc agtgatggta ttgttcctgt ttgtcgtgat gatgctgaat atcgacatcg
    11221 aggaaatgcg cgccggtttt tggcggcacg cgccggttgc cggcgtggtc ggtacattgt
    11281 tggcggttgc cctgattctg attttggtca acccgaaaac cgaccttgcc gcatttggtc
    11341 tgatgaaaga cattcccacc gattacaaca atatccgcga tttgggcagc cgtatttata
    11401 ccgaatacct gttgccgttt gagctggcgg cggtattgct gctgttgggt atggtggcgg
    11461 cgattgcgct ggttcaccgt aaaaccacca atccgaaacg catggatcct gccgaccaag
    11521 ttaaagtacg tgccgaccaa ggccgtatgc gtctggtgaa aatggaagcg gtcaaaccgc
    11581 aagttgaatc tgccgaagag agcgaagttt cagacggctt caagccggaa ggggagggta
    11641 aagcatgatt accttgacgc attatctggt attgggtgcg ctcctgttcg gtatcagcgc
    11701 aatgggtatc tttatgaacc gcaaaaacgt gctggtattg ctgatgtcca tcgagctgat
    11761 gcttttggcg gtgaacttca actttatcgc cttctcgcaa catttgggcg atactgccgg
    11821 acaaattttc gtattcttcg tattgaccgt tgccgctgcc gaatccgcca tcggtttggc
    11881 gattatggta ttggtgtacc gcaaccgaca aacgattaat gttgccgatt tggatgggtt
    11941 gaaagggtaa aggtaggttg ggtcgagacc tgacaagaca ccgatgccgt ctgaaaaccc
    12001 gataggaaaa acgatgaaat ccatagacga acaaagcctg cataatgccc gccgcctgtt
    12061 tgaaagcggc gacatcgacc gtatcgaagt cggtaccacc gcgggcctgc aacagattca
    12121 ccgttacctg ttcggcggct tatatgattt tgcgggtcaa atcagggaag acaacatttc
    12181 caaaggcggt tttcgttttg ccaacgccat gtatttaaaa gaggctttgg ttaaaatcga
    12241 gcagatgccc gagcggactt ttgaagaaat catcgccaaa tatgttgaaa tgaacattgc
    12301 ccatccgttt ttggagggta atggcagaag tacccgcatc tggctggatt tggtgctgaa
    12361 aaaaaacctg aaaaaagtcg tgaactggca aaatgtaagt aaaaccctgt atttgcaggc
    12421 gatggaacgc agccccgtca acgatttaga actgcgcttt ctgttaaagg acaacctgac
    12481 tgacgatgtg gacaaccgtg aaatcatctt taaaggtatc gagcagtcgt attattacga
    12541 agggtatgaa aaaggctgag ggtcgtctga aaagcgattt cagactgttt cagacgacct
    12601 gattcggtag gtgatcagac gggagcggat gagaaaagaa attctgggta agaataatcc
    12661 ggtctgaaat attggaagaa gaatgatgga taaaaatcag ttagaacaag aatttcataa
    12721 agccatgtta aatatttatc aggaggcttt gaatttgccg caaccttaca aggcgacacg
    12781 atttttacaa attgtaaatg aatttggtgg taaagaggcg gcggataaat tattgagtac
    12841 gggggaaaag aagactcaga ccggttttac agagctgatt ctgagtggtg gcggagtcca
    12901 cgccttgaaa tacagtatgg aatatctggt gttacaaaag ccgtggtgtg atttatttac
    12961 tgaagagcaa ttagctgtgg cacgcaaacg attggagcgt gttggatttg tttttccgaa
    13021 gtaattttgt acgaaacaaa catagatttt taaatcaatc ggattcaatc aaatgaacga
    13081 catgacttta tatctcatca ttgcccttgt tccgttggca ggctcgttga ttgcgggttt
    13141 gttcggcaac aaaatcggtc gtgccggtgc gcatactgtt acgatactcg gtgtggcggt
    13201 gtctgccgtg ctgtcggctt atgtgctgtg gggattcctc aatggcagcc gtgccaagtt
    13261 tgacgaaaac gtctatacct ggctgacaat gggcggcttg gatttttccg tcggcttctt
    13321 ggttgatacg atgacggcga tgatgatggt cgtggtaacg ggcgtgtcgt tgatggtgca
    13381 tatctacacc atcggctata tgcacgacga aaaagtcggc taccaacgct tcttcagcta
    13441 tatttctttg tttactttca gcatgttgat gctgattgtg agcaacaact tcatccagct
    13501 cttcttcggc tgggaggctg tgggcttggt gtcgtatctc ttgatcggtt tctatttcaa
    13561 acgtccaagc gcaacatttg ccaacctgaa agcctttttg atcaaccgtg tcggcgactt
    13621 cggctttttg ctcggtatcg gcttggtgct tgcctatttc ggcggcagcc tgcgctatca
    13681 agatgtattc gcttatctgc ccaacgtgca aaatgccacc atccagcttt tccccggtgt
    13741 ggaatggtcg ttgattactg tcacctgttt gctcctgttt gtcggtgcga tgggtaaatc
    13801 ggcacaattc ccactgcacg tttggctgcc tgattcgatg gaaggcccga ccccgatttc
    13861 tgcattgatt cacgccgcaa ccatggttac cgccggtttg tttatggtgt cgcgtatgtc
    13921 gccgatttat gagatgagca gcaccgcgct gtctgtcatc atggtaatcg gtgcgattac
    13981 cgccctgttt atgggtttct tgggcgtgat tcaaaacgac atcaaacgtg tggttgcgta
    14041 ttccaccctg tcgcaactgg gctatatgac cgtggctctg ggcgcgtctg cctattccgt
    14101 ggcgatgttc catgtgatga cccacgcctt ctttaaagcc ttgttgttct tggcggcggg
    14161 cagcgcgatt atcggtatgc accatgacca agatatgcgc cacatgggca atctgaaaaa
    14221 atacatgccg attacttggc tgaccatgct gatcggtaac ttgtcgctga tcggtacgcc
    14281 gttcttctcc ggcttctatt ccaaagattc gattatcgaa gcggcgaaat acagcaccct
    14341 gccgggcagc ggctttgcct attttgccgt cctcgccagc gtttttgtta ctgcgttcta
    14401 cgcattccgc caatacttta tggtgttcca cggcgaagag aaatggcgca gcttgcctga
    14461 acatcatgac gatcatcatg gcgaagaaca tcacagcttg ggcaaaaacg acaatccgca
    14521 cgaaagcccg ttggttgtta ccctgccttt gattttgctt gccattccgt ccgtcatcat
    14581 cggctacctt gccatcgagc ctatgcttta cggcgatttc ttcaaagatg tgattttcgt
    14641 caacgccgac gcgcatccga ccatgcacat catgaaggaa gagttccacg gcgcattggc
    14701 aatggtgtcg cacagcctgc attcgcccgt actctacctt gctatcgcag gcgtgttgag
    14761 cgcatggctt ttgtacgtca aactgccgca cctgccagcg aaaattgcac agacgttccg
    14821 tccggtttac gttttgtttg aaaacaaata ctacctcgac gtcctgtatt tcaacgtctt
    14881 tgccaaaggc acgcgcgcat tgggcacttt cttctggaaa gtcggcgata ccgccattat
    14941 cgacaacggc atcgtcaacg gctctgccaa actggtcggc gcgattgccg cgcaagtgcg
    15001 taaagctcaa accggcttta tctataccta cgccgccgct atggtattcg gcgtattggt
    15061 actgctcggc atgaccttct ggggattgtt ccgataagaa taaggtttca gacggcctta
    15121 aaccttcagg ccgtctgaaa cgaagaaata tccacataaa cacattttta ttttaaccac
    15181 aggttaacca ctatgttttc caactaccta ctcagcttgg caatatggat acccatcgcc
    15241 gcaggcgtgc tggttttggc aacggggtcg gacagccgtg cgccgtttgc ccgcgtgctc
    15301 gccttcatgg gtgcgcttgc cggtttcttg gtaacactgc ccctgtttac cggtttcgac
    15361 cgtttgagcg gcggctatca atttaccgag ttccacgagt ggattccgct tctgaaaatc
    15421 aactacgcat tgggcgtgga cggtatttca gtgctcttta tcatcttgaa tgcgtttatt
    15481 acgctgttgg tggtattggc aggttgggaa gtcattcaga aacgtccggc gcagtatatg
    15541 gcggcattcc tgatcatgtc gggtttgatt aacggcgcgt ttgccgcgca ggatgcgatt
    15601 ctgttttatg tgttcttcga gggtatgctg attccgctgt acctgattat cggtgtatgg
    15661 ggcggtccgc gccgcgtcta tgcgtcggtc aagctcttcc tctacacgct gatgggttcg
    15721 ctcctgatgc tggttgcgat ggtttacctt tattatcaaa caggcagctt ctctattgtc
    15781 gatttccaaa acatcgaaca gattccgttg ggcgtacaac agcttttgtt tgtggcgttc
    15841 ttcctgtcat ttgccgtaaa agtgccgatg ttccctgtgc acacttggtt gccggatgcc
    15901 cacgttgaag cgccgaccgg cggttcgatg gtgttggcgg ccattacgct gaaactgggt
    15961 gcgtatggtt tcttgcgctt tatcctgccg attatgccgg atgcggcacg ctattttgcc
    16021 cccgtgatca tcgtattaag tctgattgcc gtgatttata tcggtatggt ggctttggtg
    16081 caaaccgata tgaaaaaact ggtggcgtat tcgtccatca gccatatggg ttttgtaacg
    16141 cttgggatgt ttttgtttgt tgacgggcag ttggacgact gggcattgaa aggtgcaatc
    16201 attcaaatga tttcgcacgg tttcgtgtct gccgcgatgt ttatgtgtat cggcgtgatg
    16261 tacgaccgcc tgcacacgcg caatattgct gattatggcg gcgtggtcaa tgtgatgccc
    16321 aagtttgcgg cgtttatgat gctgttcggt atggcgaacg cgggtttgcc tgcgacttcc
    16381 ggcttcgtgg gcgagtttat ggtgattatg ggcgcggtca aagtgaattt ctgggtcggc
    16441 gcgttggccg ccatgaccct gatttacggt gcatcttata ccctgtggat gtacaaacgc
    16501 gttatttttg gtgcgatcca caatccgcac gttgccgaaa tgcaagacat caattgccgc
    16561 gaatttgcga ttttggcaat tttggcggtg gctgttttgg gtatgggcct gtatccgaac
    16621 gcatttatcg aagtggtgca tcaggcggca aacgatttga ttgcccatgt ggcacaaagc
    16681 aagatttgag gtgtgtaaat gaactggtct gatttgaatt taatgcccgc catgcccgaa
    16741 atcgtgctgc tgtcgctgct ggtgttattg ttgctggcgg acttgtgggt cagtgatgac
    16801 aaacgccggt ggacgcatta cggcgcgttg gcaacggtgg cggttacggc tgtggtgcag
    16861 ttggcggtgt gggaacaggg cagcacgtct tcgttcaacg ggatgtatat tgcagacggt
    16921 atgtcgcgtt tggcaaaaat ggttttatat gccttgacct ttgccctgtt tgtctatgcc
    16981 aagccctaca accaagtgcg cggtattttt aaaggcgagt tttacaccct gtcattgttt
    17041 gccctgttgg gtatgagtgt gatggtgagc gcggggcatt ttttaactgc ctatatcggt
    17101 ttggaactct tgtcgcttgc cctttacgcc ctgattgccc tgcgccgcga ttccggcttt
    17161 gccgccgaag ccgccttgaa atattttgtt ttgggcgcgc tggcatccgg cctgctgctc
    17221 tacggtattt ctatggttta cggcgcaacc ggttcgctgg aatttgccgg cgtgctcgcc
    17281 tcttccttca atgaagaagc caacgaatgg ctgttgaaac tgggtttggt gtttatcgtc
    17341 gtcgccgtcg cgttcaaact cggtgcggtg ccgttccata tgtgggtgcc cgacgtgtat
    17401 cacggcgcgc ccacttctgt taccgccttg gtcggcactg ccccgaaaat cgccgccgtc
    17461 gttttcactt tccgcatcct cgttaccggg ctgggaaccg tgcatcatga ctggtctctg
    17521 atgtttgccc tgcttgccgc cgcctcgctg ctggtcggca accttgccgc catcatgcag
    17581 accaatatca aacgtatgtt cgcctattcc accgtatcgc atatgggttt catcctgttg
    17641 gcgtttatgg cgggcgcggt cggctttgcg gcgggcctct attacgccat tacctacgcg
    17701 ctgatggcgg cggcagggtt cggagtgttg atggtgttgt cggacgggga caacgagtgc
    17761 gaaaacatca gcgatttggc agggttgaac caacaccgcg tatggcttgc ctttttgatg
    17821 ctgctggtta tgttctctat ggcgggcatt ccgccgctga tgggttttta cgccaaattc
    17881 ggcgtgatta tggcactctt gaaacaaggc catgtttggt tgtctgtatt tgccgtcatc
    17941 atgtcgctga ttggtgcgtt ctactacctg cgcgtggtca aagtcatcta cttcgatgtg
    18001 cctgatcatg accagccggt cggcagcaac tatgccgcca aatttgttct gacggtcaat
    18061 gccttcttgc tgctcctgtg gggcatcatg ccgcaaaccg ttatcgactg gtgcgccaag
    18121 gcgttggaga acacgctgta agccgccgca acggcagccg tgtcagaggc tgccgttttt
    18181 gttaagatat gccgttccgc aacgcggttc agacggcatc gccgccgaca acgcctaaac
    18241 agaaagccca ccatgaccgc atccatgtac atccttttgg tcttggcact catctttgcc
    18301 aacgccccct tcctcacgac cagactgttc ggcgtggccg cactcaagcg caaacatttc
    18361 ggacaccaca tgatcgagct ggcggcaggt ttcgcgctga ccgccgttct tgcctacatc
    18421 ctcgaatccc gtgcaggatc ggtacacgat cagggttggg agttttatgc cacagtcgtc
    18481 tgcctgtacc tgatttttgc gtttccatgt tttgtgtggc ggtatttttg gcacacgcgc
    18541 aacagggaat agacaagcat aggaatgccg tctgaaaccc tttcagacgg catttgtttc
    18601 attcaagtgc aggccggcat cgctgtgccg gcacgtttca gccggcgata tacgccggtt
    18661 ttaatatttg cgggcgactg caaattctgc caactgccgc aggcgcaggg ctttgtcgcc
    18721 gaagggttcg agcagcgcga ccgcttcggc aaccagtttg tgtgcgtatg agcgcgccgc
    18781 ttccaagccc atcagtttca cataagtcgg cttgtcgttg tctgcgtctt tgcccgccgt
    18841 tttgcccaaa gtcgccgtgt ccgcttcaca atccaacaca tcgtcaatga cttggaacgc
    18901 cagccccagt tttgccgcgt aagcgtccaa tacggaaagt tccgcatctg acagatcagg
    18961 acacgccgtc gcccccaata aaaccgccgc acggattagc gcacccgttt tcaggctgtg
    19021 catctgttcc aaatcggctt gaaccatttg tttgccgaca ttcgccaaat cgattgcctg
    19081 accgcccgcc atacccctgc tgccgcccgc tttcgccaac accgacaaca ttgccaactg
    19141 gcgtgcggcg ggcagttctg tcggacggct caacacgtca aatgcctgtg tctgcaaagc
    19201 gtcgccggtc agaagggcgg tcgcttcgcc atatttgatg tggcaagtcg gtttgccgcg
    19261 ccgcaggctg tcgttgtcca tcgccggcat atcgtcgtga accaaagaat agacgtggat
    19321 catttcgatt gccgccattg cctgttctac tgcttcatgc acggcttcgc ctaattccga
    19381 agctgccaga accagcatcg gccgcagacg cttaccgccg tccaaagccg cataacgcat
    19441 cgcttcgtgc agtgtgtgcg gtatttcccc ctcagacggt aaaaaccgtt caagcagcag
    19501 ctctgtttgc gcctgcgccc tctgttgcca cgttttcaaa tcattcgtcg gattcaaggt
    19561 ttaactcctt cagcccgtct gtgtctaaaa cctgtagctt ttgttcgact tgtgccagtt
    19621 tggtttggca gtacctgacc agttcgttgc cttcctgata ggcggcaagc gcgtcttcca
    19681 agggcatttc gccctgcata gactgcgtca gcgattcgag gcgcgacaag gcttcttcaa
    19741 acgatttcgg ggcgtttttc ttcatcgtat ttccttttcg gttgaaaccc cgccctttag
    19801 ggcggcagga tcagacttta tttgggaggg gtgtaaccct ttccaaatca gggcaataca
    19861 tagggcggtg ctttatgtgc cgtcctgtgt gttggaacat agtttcggat gttccggtaa
    19921 aaagcggatt gtagcatttt tgaaaaacgg atgccgtctg aaacccgaat ccggcttcag
    19981 acggcatttt ttccgcccag gcggcaaggc gttacccggg cagttcgtcg gtgatgccct
    20041 gcaaaaaggc gaggcgttcg gggcttgccg ccccggtttg cgcggcggct ttgaaggcgc
    20101 agccgggttc ggcgcggtgg gtgcagttgt ggaagcggca ttgcccgaca aggtggcgga
    20161 aatcggggaa atagcgcggc aaatcggcgg cttggaggtg gtgtaaacca aattcttgca
    20221 aacccgggga gtcgatgagt tgggtttcgc cgttcaaatc ataaagccgg gcgtgggtgg
    20281 tggtgtgttt tcccgagtcg agtgcggcgg aaatgtcgcc ggtgcgggcg gtttggctgc
    20341 ccaaaagggc gttggtcagg gtggatttgc ccataccgct ctgcccgagc aggatgttgc
    20401 tgtgcccttg cagggcgggg cgcaggctgc cggcgttttc cagtgcgcgg gtttcgatga
    20461 cgggataacc cagcgtttcg tagaatttga gtttttcgcg ccaaagggcg gtttcgggca
    20521 ggtcggcttt gttcaggacg atgacggctt caataccggc ggcttcggcg gcaagcaggg
    20581 cgcgttgcag cagccgcacg ctcggactcg ggacggcggc ggttacgatg aggagttggg
    20641 taacgttggc ggcgatgagt ttggttttcc acgcgtcttg gcggtagagc aggctttggc
    20701 gcggtaaaaa atcttcaatc acaacttgtt cggcgttgac ggggctgatg cggacgcggt
    20761 cgccgcaggc gaaatcgacg cgttttttgc gggtgctggc ttcgtaggtt gtgccgtcgg
    20821 gcgtgcggac aatgtagcgg cggccgtagc tggcggtaat ttgggcggtg tcgttcatgg
    20881 tttctttggg gttgggtgtg ggaatgccgt ctgaaaacgg gtgttcggac ggcatcggtt
    20941 cagtcgtgct gccactcgac gtgttcgttg aggaagccgc cgctctggtg cgcccagagt
    21001 ttggcgtaaa gcccgcgttt ttcgaggagt tcggcgtgtg tgccttcttc gatgatgcgg
    21061 cctttgtcga ggacgacgag cctgtccatt gcggcgatgg tggagaggcg gtgggcgatg
    21121 gcgatgacgg ttttgccgtc catcattttg tcgaggcttt cttggatggc ggcttcgact
    21181 tcggaatcga gcgcgctggt ggcttcgtcc aaaagaagaa tcggtgcgtc tttgagcatc
    21241 acgcgggcga tggcgatgcg ctggcgttgc ccgccggaga gtttcacgcc gcgttcgccg
    21301 acgtgtgcgt cgtagccgcg ccgccctttg gcatcggaaa ggtcggggat gaagccggcg
    21361 gcttcggcgc gttcggcggc agaaaccatt tcggcatcgg tcgcgtcggg gcggccgtaa
    21421 ataatgttgt cgcgcacgga acggtgcagc agcgaggtat cttgcgtgac caaaccgatt
    21481 tgggcgcgta aagattcttg ggtaacgccg cttatgtcct gcccgtcgat cgaaaccgtg
    21541 ccgctttgcg gttcgtagaa gcgcaaaagc aggttgacga tggtggattt gcccgcgccg
    21601 ctgcgtccga tcaagccgac tttttcgccc gggcggatgg tgaggttgaa gccgttgagc
    21661 agcggtttgc ccgcttcgta ggagaaatcg acgtgttcga acttgatttc gccttgcgac
    21721 actttcagcg gcagggcgtt cggcttgtcg aggatggtgt gcggtttgga cagggttgcc
    21781 atgccgtcgc cgacggtgcc gatgttttca aacagccgcg cggattccca cataatgtat
    21841 tgcgacaaac cgttgacgcg caacgccatc gcggtggcgg tggcgcccgc gcccacgccg
    21901 acctgtccgt tgtgccaaag ccagatgccc agtgcggcgg tggagagggt cagggaggtg
    21961 ttgacgatga agctgcacga atgcagcagc gtcgccagcc gcatttgggc gcgcaccgta
    22021 accataaatt cttccatcga ctgcttggca taggcggctt cacgcgcgcc gtgggagaag
    22081 agtttgacgg tggcgatatt ggaataggca tcggtaatgc ggccggtcat cagcgagcgg
    22141 gcatccgcct gccatgcggc ggtttgcccc aatttgggaa tcagcaggcg catcaccgaa
    22201 gcgaaaccga caatccagcc gataaagggc agcagcagcc atgagtcgag cgaggcgaga
    22261 atcacgccgg aggtaatgaa atacaccgac acataaacga ccatatcggc aaccgtcatc
    22321 accgcgtcgc gcaacgccag cgcggtctgc atgactttgg cggacacgcg tccggcaaat
    22381 tcgtcctgat aaaaaccgag gctttggttc agcatcaggc ggtggaagtt ccagcgcagg
    22441 cgcatgggga acacgccctg aagggtttgc aggcgcacgt tggacgcggc aaacgcccac
    22501 acgaccgaaa ataccatcat cgccgccatt gccgccagtt cccaaccttt ttcggcaaac
    22561 agttcggcgg gcgcgtattt gccgagccac tccacgattt tgcccataaa ttgaaaaatc
    22621 agggcttcca taatgccgat gccggcggtc agcgcagcca gggcggctat ccatttccgc
    22681 acgccggcca tgctgctcca gacaaaccgc cacaagcctt tttctggcgt tttcggggcg
    22741 gcttcgggat aagggtcgat tcgggactcg aaccaggaaa atattttgtt caacattgtt
    22801 ttcgatttcg gtaaaacagt ttcagacggc atcaaacaca atgccgtctg aaaggaagga
    22861 caataacgcc attttacggg aaaagccgtc gggaagacag cgcgaggcgg aaacgcaggg
    22921 tttcgtcagg gcaaacgccg cgccgccttc agacggcatt atttcagcag gtttttcaaa
    22981 gcaaggcgca cgccttcgcc cacgtccgtc ccctccggaa cgcctttgac cgccgctttt
    23041 gcttcgcgtt cgctgtaacc cagcgcaagc agcgtgctga cgatgtcttc cgtttcgtcg
    23101 gcggcgggtg cggcggcaaa cagcccgtcc gttaccgcat gcgcgaccag cttgccgcgc
    23161 agttccaaga ccatacgttc ggcggttttt ttgccgattc ccggcgcgga agacaggcgt
    23221 ttgacatctt cttcggcaac cgcccgcgcc agctcgtctg ccgtcatcgc tgacaaaatg
    23281 cccaaagccg ttttcgcgcc gatgccgccg accttaatca gttggcggaa ggtcttgcgt
    23341 tcttccgcag tggcaaaacc aaataaaaga tgtgcgtctt cccgaatgat aagctgggta
    23401 aacagttgta cgctttcacc cacgggcggc aggttgtaga aggtctgcat cgatacgtcg
    23461 gcctcatagc cgacaccgtt gacatcgatg acgatttgcg gagggttttt ttcaaccagt
    23521 ttgccggtca gtctgctgat catgtgtgcc gaatcctgaa gtgtcgggtg caaaatgccg
    23581 tctgaaaccg gttcgggctt cagacggcac ggattgtatc aaattcagtc gtcgcggcgg
    23641 gaggaaatca cgcggccggt acgggcatcg acaacgactt tgtattcctg tccgtttttg
    23701 acgatttcga catcatagtg cggacggccg ttgtcgtgtt cgagatcgat gtcggtgatt
    23761 ttgccgccga cacgcgccaa cgctgctttt tcggcttggg cgcggctgat gattttgtct
    23821 tgtttgttgt gttggtgtgc ggcgtgtccg tggtcgtcat cgccgtgtcc gtcgtggtgg
    23881 gcgagcgcgg gggcggaaat gctcagcagt gcggttgcgg cggaggtcaa gagaaggtgt
    23941 ttgatgttca tattttgcct ttgtaaatcg tgggttggaa aatgtggata ttaataaggt
    24001 atcaaataac cgtcagccgg cggtcaatac cgcccgaacc ataccgcgcg cctgagcttc
    24061 ggcttcggcg gcgcgttcct gcgaggtaaa cggtcccatt ttgacgacgt attcgtaacg
    24121 gcgtttttca accgagaggt tcgtacccga tgacgaaacg gcgaagtttt gggcggcttg
    24181 gttcagatag gcttgtgctt cgtgttccgt accgaaagat ttcaagtcga taaagatgtc
    24241 tttgttttcg gcaaccggtg cggattggcc cgggacgatt tgttcgattt tgacgtgtgc
    24301 cgtcccttgg ttgacaaagc ccaatttttg cgcggcggct ttggatacgt cgatgatgcg
    24361 gttgccgtgg aaggggccgc ggtcgttgac gcggacgatg acgcttttgc cgtttttggt
    24421 attggttacg cgcacatagc tggggatggg cagggttttg tgggcggcgg taaaggcgtt
    24481 catatcgtat cgttctccgc cggaagtttt gcgcccgtga aacctgccgc cgtaccacga
    24541 ggcgttgccg gtttgcgtga attcggcgac ttggtttttc ggcgtgtagc gttttccggc
    24601 gactttgtag ctgcggttgg cggaggcgtg cagtttttct gccttgacca ctgcgtcggc
    24661 ggatgccgtc tgaagggagt gtgtgccgaa tgcggcggtg agaaggaaaa gggtttttcg
    24721 ggttaaagtc aaaacgtgtt ccgttcttga gttgaagacg aatgggcatc atgcccgccg
    24781 gatacgttcc gaaccgccgt acagtgcgga cggcggttcg gaatgtgtcc ggataggttt
    24841 tcagacggca tgaacctgcg ttcaaacgcc gcctgcgtaa ccgtgttgcc gccacgcttc
    24901 aaagagaatc acggcgacgg tgttggaaag gttcatactc cggctgccgg gctgcatcgg
    24961 caggcggatt ttttgcgcgg cgggcaggct gtcgaggatg tcggcaggca gtccgcgcgt
    25021 ttccggcccg aacagtaaaa cgtcgccttt ttgaaacgcg gtttcatcgg ggcgcgccgt
    25081 gcctttggtg gtcagggcga aaatgcgcct gcctgcgagt gccttgaggc agtcgtcgaa
    25141 gttttcgtgc accgtcaggc tggcgaactc gtggtagtcg agcccggcgc gtttcatttt
    25201 ggcggaatcc aatgggaagc cgagcggttt gacaaggtgc aaatccgcgc cggtattggc
    25261 gcacaggcgg atgatgttgc ccgtgttcgg cgggatttcc ggctggtata aaacgatggt
    25321 aaacataaat atcaatcact tataggcgcg taaccttgcc acaaggcgga tggggtgtca
    25381 aaaaatttag ttattttttc attggcgtgc gtgccagcgt ccagcagcag attcggtttg
    25441 cgcccgattt tttcagcgtc tttgccaatt cgtccagcgt cgcgccggtg gtaaagacat
    25501 cgtcgattaa caaaatatta cagtttttcg gcggcggtgt gcggatttca aaggcgtttt
    25561 tgatatttcg ccgccgttcg ccgcctttga gcgtgctttg cggcggacgg tggtgtcgga
    25621 atacggtgtg tcggggcagt atccgccagc cgtagcgttg cgccaacaac ccgacgatgc
    25681 cctcgctttg gttgaacccg cgttgcagca gccgctccct gcttagcgga acgggcagga
    25741 cgaaatcgaa acattcgtct gcaagcaggt cgggcggatt ctgcatcatc aggtctgcca
    25801 gcggctgcac catgcccaaa tcagccaagt gcttcagcgc gcgtatcata ttgctgacgg
    25861 gcggttcgta atgcagcgat gcccacatcc ggtcgaatgc gggcggtttt ttctgacagc
    25921 cgccgcatac cgcgccgcct tgaacgtgcc tgaaacacag ggggcagctg tttgccgcgt
    25981 cggtgcggta tgccgccaaa tcgttgcggc agccggcgca gatgccgtct gaaacgccgg
    26041 acgaaccgtg gcataatacg caacgcctga tagtgggcgc gtctgcgatg cgccgccaac
    26101 aagagagaaa atccatgcct gatgccgtca aaaaagttta cctgatacac ggttgggggg
    26161 cgaaccgcca cgtgttcgac gatttgatgc cgcgcctgcc tgcaacgtgg ccggtgtccg
    26221 ccgtcgattt gcccggacac ggcgacgcgc ccttcgcgca acccttcgat attgaggcag
    26281 ccgccgacgc tgttgccgca caaatcgata cgcccgccga cattctcggc tggtcgctcg
    26341 gcggattggt cgcgctgtat ctggcggcgc gccatcccga caaagtccgt tcgctctgcc
    26401 tgacggcgag tttcgcacgg ctgacggctg acgaagacta tcccgaaggg cttgccgcgc
    26461 ctgcattggg caaaatggtc ggcgcgttcc gaacggatta tgccaaacat atcaaacagt
    26521 ttctacaact ccagcttctg cacacgcctg atgcggacgg aatcataggc agaatcctgc
    26581 ccgatttggc gcgctgcggc acgccctccg ccttacagga agctttggac gcggcggaaa
    26641 gggcggatgc gcggcatttg ttggacaaga tagatgttcc ggtactgctg gtgttcggta
    26701 gcaaagacgc gattacgccg ccgcgcatgg gcgagtatct gcaccgccgt ttgaaaggca
    26761 gcaggctggt tgtgatggaa aaggcggcgc atgcgccgtt tttgagccat gcggaagcgt
    26821 ttgccgcgct gtaccgcgac tttgtcgaag gggtttgaca tgaatccgca ggacaaatgc
    26881 tggcaggttc accgccatct cgccgaatat gccgaccaac gcctgacact cgtccgcaac
    26941 gcgcccaagt atatcctgct tgcaggtgcg gatgcggaca tcagccgcag cctgctggcg
    27001 aaacgctatc cgcaggcggt atttgaagaa tacgattccc gtgcggattt tttggcggct
    27061 gccgctgccg cccgcaaagg cggtttttgg caaaggttta cgggtaaggg cgtggtgcaa
    27121 cactgccaat ccccgatcgc gccgctgccc gaagcgtgtg ccgatatgtt gtggtcgaat
    27181 ctcggactgt tggcggcgga acaaatcctt cctgtgctgc acaactgggc gcgcgccttg
    27241 aagacggacg ggctgctgtt ttttacctgc ttcgggcgcg acacttttac cgaactgaaa
    27301 tgccgtctga aagaaaacgg cattgaaagc cgcagcgtga tgtttcccga tatgcacgac
    27361 ttgggcgata tgcttgctga aaacggcttt tacgaccccg ttaccgatac ggcgaagctg
    27421 gtgttggatt acaaaaaggc ggaaacgttt tgggcggata tggacacgct gggcgtttgg
    27481 cgggcgatgg cgtggaacga tgaaaacgcc gcgcgttcgt gtgtcgggac aatatttgag
    27541 cgggaaggcg gtttgggcat tacgctggaa acggtgtacg gacacgccgt gaaaaaactg
    27601 atgctgccgc aaggggagaa cgtggtgcag ttttttccga agagatgatg tgcagatgcc
    27661 gtctgaagcc gtttccaagt ttcagacggc atttgtttgt gaaaaccgac agaactaaag
    27721 gaaatgccga tgtatagtga attaaattta aaccagtaca gcgttgcctc gccttagctc
    27781 aaagagaacg attctctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta
    27841 ctgtctgcgg cttcgccgcc ttgtcctgat ttttgttaat ccactatatg ctgatgccgg
    27901 agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc aggttacccg
    27961 agttgaagaa cacggtggca aaaaaaacac atgcgaccct gctggctttg ggctggcagg
    28021 gcaacaaacc gcttggggcg gaggagctgg cggatttgaa accgctttac aaagacttaa
    28081 agaataatat tggaaatatt gtatgaacaa aaaattaaac tatattttta tgttggactg
    28141 tttagggttg gtgatattgt ttacttgtat aatagctact tttgaaagag attatggatt
    28201 taaaattttt actaattcta agaggcctga attttattat tggattggaa tgttttatta
    28261 tggaattatt tcttgctggt ttgattatca attaatttca acaaaggcga attcgtataa
    28321 aagaaaagtt aaacaatata aaattttttc agtaatattt tcagttttga tatttatttc
    28381 tactatagta aaactttaaa ttttggagca aaaatttatg agcgattcaa ttgaatatgt
    28441 attgggaacg cggtctgcac atgtataagg caagtgccgt cgtgccgacg ggatatgtac
    28501 gggttgggaa taccgcgccg ctggtcggcg aagacacgca acggtatgcc tctttttggg
    28561 gcgacggcta cgacgtgtac cgtcagttga gatggcagca gatatccgaa aaacagagaa
    28621 aggcattcaa aaaagccgcc aaaagcaaaa agaccgtgat gtttgccgga cgggaatacg
    28681 gcatatccaa acaaaatttg agcgatgttt gggacgattt tgaagacgcg atggaactga
    28741 aggcgtttcc ctgcctgtct tcactgtttc tgaccaaatg gcataaaaat ctatatgata
    28801 gtggattaac aaaaaccagt acag
//
LOCUS       DAWHRA010000005        28788 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWHRA010000005 DAWHRA010000000
VERSION     DAWHRA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28788
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.005"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1091
                     /gene="mafB"
                     /locus_tag="WIB30_000176"
     CDS             <1..1091
                     /gene="mafB"
                     /locus_tag="WIB30_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ3796320.1"
                     /translation="GIGDILYGTRYAIDKAAMRNIAPLPAEGKFAAIGGLGSVAGFEK
                     NTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKAAVSGDFSKSYTCSF
                     HGSTLVKTTDGYKAIAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYQETVYIEVSDG
                     LGKIQTLVSNRIHPFYSDGKWIKAEDLKAGNRLLSESGKTQTVRNIVVKPTPLKAYNL
                     TVADWHTYFVKGDKAETEGVWVHNDCPYGSSGAKGKSGSERNTAKRGTFRQETLKNAW
                     DLAADGSQPNSKKCPTCGKDVYGNPHNKEKRNTKDGWDGSHNPSWSNRQHSSDRKEQL
                     NDYNKGVSLECRHCNRSGGNKDERFKKK"
     gene            1088..1633
                     /locus_tag="WIB30_000177"
     CDS             1088..1633
                     /locus_tag="WIB30_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ3796321.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYTGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRKPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNTVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMNRKPFVW"
     gene            1769..2359
                     /locus_tag="WIB30_000178"
     CDS             1769..2359
                     /locus_tag="WIB30_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796322.1"
                     /translation="MVKTADGYKAIARIRVGDRVFAKDETSGVTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGKTQTVRNIIVKPTPLKAYNLTVADWHTYFVKGNQAETEGVWVHNDC
                     PPYKRPNNATTKAQRESVQGKPCVEYGKLAEKMIADHKKPLVVEYYETGTIDKSKMRA
                     IESVQPQCPTCSAKQGGRLSQYSKEQKRKLSNGNKK"
     gene            2343..2696
                     /locus_tag="WIB30_000179"
     CDS             2343..2696
                     /locus_tag="WIB30_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796323.1"
                     /translation="MGIKNEQIIICKKYNTEIYPISDVSKIGVAENVKQANLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            2686..3009
                     /locus_tag="WIB30_000180"
     CDS             2686..3009
                     /locus_tag="WIB30_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ3796324.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQNEFGEMWTCYCSVNIVLSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            3068..3295
                     /locus_tag="WIB30_000181"
     CDS             3068..3295
                     /locus_tag="WIB30_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796325.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            3422..3965
                     /locus_tag="WIB30_000182"
                     /pseudo
     CDS             3422..3965
                     /locus_tag="WIB30_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
     gene            4131..4730
                     /locus_tag="WIB30_000183"
     CDS             4131..4730
                     /locus_tag="WIB30_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ3796326.1"
                     /translation="MAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYQETVYIEISDG
                     IGNNQTLISNRIHPFYSGGKWIKAEDLKAGSRLFAESGKTQTVRNIVVKPTPLKAYNL
                     TVADWHTYFVKGNQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQISAESKIAELTN
                     KIIPGMSKNERLKIKQKIKNIAKNANRKTKGEEHGRRGR"
     gene            4711..5166
                     /locus_tag="WIB30_000184"
     CDS             4711..5166
                     /locus_tag="WIB30_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796327.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVEKNKIISIFCYQELYFMGVNIIGLNFEIFKQLFHYPKLNDID
                     KCYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSIEPYYDE"
     gene            5387..5746
                     /locus_tag="WIB30_000185"
     CDS             5387..5746
                     /locus_tag="WIB30_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796328.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWIFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDS
                     YKVNWKINNGNLIQTTN"
     gene            5758..6093
                     /locus_tag="WIB30_000186"
     CDS             5758..6093
                     /locus_tag="WIB30_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796329.1"
                     /translation="MMADKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLNIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKHP"
     gene            6242..6736
                     /locus_tag="WIB30_000187"
     CDS             6242..6736
                     /locus_tag="WIB30_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796330.1"
                     /translation="MKNRFAKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWRELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMEIFKMSVLEDWERSGKPLDWKKWQRQYRSEAFELVKKLNR
                     SKQE"
     gene            6856..7989
                     /locus_tag="WIB30_000188"
     CDS             6856..7989
                     /locus_tag="WIB30_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796331.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDKLPDFPLNLENLEKEVSRYAEAAARRSGDKRGLSY"
     gene            complement(8076..8351)
                     /locus_tag="WIB30_000189"
     CDS             complement(8076..8351)
                     /locus_tag="WIB30_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796332.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLMCISVFWIVVFNL"
     gene            complement(8368..9135)
                     /locus_tag="WIB30_000190"
     CDS             complement(8368..9135)
                     /locus_tag="WIB30_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ3796333.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACHLNQDLGKSLLDLGFKKIPSVYPE
                     YEAYEDKRSIPENPYIHELQYIKKGENPAIITHQNYHRYGENDYSTDIGSCINGFTVQ
                     YYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(9599..9874)
                     /locus_tag="WIB30_000191"
     CDS             complement(9599..9874)
                     /locus_tag="WIB30_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ3796334.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(9871..11154)
                     /locus_tag="WIB30_000192"
     CDS             complement(9871..11154)
                     /locus_tag="WIB30_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3796335.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(11310..12728)
                     /gene="glnA"
                     /locus_tag="WIB30_000193"
     CDS             complement(11310..12728)
                     /gene="glnA"
                     /locus_tag="WIB30_000193"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ3796336.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDTTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDAGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(13002..13379)
                     /locus_tag="WIB30_000194"
     CDS             complement(13002..13379)
                     /locus_tag="WIB30_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796337.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVLVDSNTATFGDADSRTDNSA
                     WFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPSYAE
                     WNYVIPGTLGEAQYKFVCNGYPR"
     gene            13550..14359
                     /gene="aroE"
                     /locus_tag="WIB30_000195"
     CDS             13550..14359
                     /gene="aroE"
                     /locus_tag="WIB30_000195"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ3796338.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGLGLVGDIVKVQHTEIEGKNILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELSRLFGIEAVPMADLNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAL"
     gene            14362..15063
                     /gene="mtgA"
                     /locus_tag="WIB30_000196"
     CDS             14362..15063
                     /gene="mtgA"
                     /locus_tag="WIB30_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ3796339.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LKRMGSAELPESDTD"
     gene            complement(15266..15341)
                     /locus_tag="WIB30_000197"
     tRNA            complement(15266..15341)
                     /locus_tag="WIB30_000197"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15306..15308),aa:Phe,seq:gaa)
     gene            complement(15532..16266)
                     /gene="lptB"
                     /locus_tag="WIB30_000198"
     CDS             complement(15532..16266)
                     /gene="lptB"
                     /locus_tag="WIB30_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ3796340.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(16340..16870)
                     /gene="lptA"
                     /locus_tag="WIB30_000199"
     CDS             complement(16340..16870)
                     /gene="lptA"
                     /locus_tag="WIB30_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ3796341.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVIIQPSSTQKSE"
     gene            complement(16851..17432)
                     /gene="lptC"
                     /locus_tag="WIB30_000200"
     CDS             complement(16851..17432)
                     /gene="lptC"
                     /locus_tag="WIB30_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ3796342.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(17429..17965)
                     /locus_tag="WIB30_000201"
     CDS             complement(17429..17965)
                     /locus_tag="WIB30_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3796343.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(18183..19157)
                     /locus_tag="WIB30_000202"
     CDS             complement(18183..19157)
                     /locus_tag="WIB30_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ3796344.1"
                     /translation="MAENGKYLDWAREVLHAEAEGLREIAAELDKNFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQCRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            19254..20309
                     /gene="tal"
                     /locus_tag="WIB30_000203"
     CDS             19254..20309
                     /gene="tal"
                     /locus_tag="WIB30_000203"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ3796345.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALETKGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLA"
     gene            20553..20912
                     /locus_tag="WIB30_000204"
     CDS             20553..20912
                     /locus_tag="WIB30_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ3796346.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     gene            20986..21183
                     /locus_tag="WIB30_000205"
     CDS             20986..21183
                     /locus_tag="WIB30_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796347.1"
                     /translation="MTKLVSRNASNERIADRAFDFFSPALMDGSATEEQYNALYDLTL
                     LEEPGMELNKDEIMALINSLK"
     gene            21200..22087
                     /gene="gluQRS"
                     /locus_tag="WIB30_000206"
     CDS             21200..22087
                     /gene="gluQRS"
                     /locus_tag="WIB30_000206"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ3796348.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGKWLIRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPALQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAVTAP"
     gene            complement(22157..23167)
                     /gene="dusA"
                     /locus_tag="WIB30_000207"
     CDS             complement(22157..23167)
                     /gene="dusA"
                     /locus_tag="WIB30_000207"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ3796349.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANM
                     RE"
     gene            complement(23198..23689)
                     /locus_tag="WIB30_000208"
     CDS             complement(23198..23689)
                     /locus_tag="WIB30_000208"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ3796350.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(23762..24520)
                     /locus_tag="WIB30_000209"
     CDS             complement(23762..24520)
                     /locus_tag="WIB30_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3796351.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSEDELHKFYEQQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(24591..25457)
                     /locus_tag="WIB30_000210"
     CDS             complement(24591..25457)
                     /locus_tag="WIB30_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3796352.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(25506..25784)
                     /locus_tag="WIB30_000211"
     CDS             complement(25506..25784)
                     /locus_tag="WIB30_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ3796353.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(25784..26074)
                     /locus_tag="WIB30_000212"
     CDS             complement(25784..26074)
                     /locus_tag="WIB30_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ3796354.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(26077..26607)
                     /locus_tag="WIB30_000213"
     CDS             complement(26077..26607)
                     /locus_tag="WIB30_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ3796355.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            26813..>28788
                     /locus_tag="WIB30_000214"
     CDS             26813..>28788
                     /locus_tag="WIB30_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ3796356.1"
                     /translation="MPAGRLPRRCPMMTKFTDCTRSNRIQPPTHRGYILKNNRQIKLI
                     AASVAVAASFQAHAGLGGLNIQSNLDEPFSGSITVTGEEAKALLGGGSVTVSEKGLTA
                     KVHKLGDKAVIAVSSAQAVRDPVLVFRIGAGAQVREYTAILDPVGYSPKTKSALSDGK
                     THRKTAPTAESQENQNAKALRKTDKKDSANAAVKPAYNGKTHTVRKGETVKQIAAAIR
                     PKHLTLEQVADALLKANPNVSAHGRLRAGSVLHIPNLNRIKAEQPKPQTAKPKAETAS
                     MPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAVRPEPVPAANTAASETAAESAPQEA
                     AASAIDTPTDETGNAVSEPVEQVSAEEETESGLFGGSYTLLLAGGGAALIALLLLLRL
                     AQSKRARRTEESVPEEEPDLDDAADDGIEITFAEVETPATPEPAPKNDVNDTLALDGE
                     SEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQNGILSGALTQDEETQKRADADWNAI
                     ESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTAENKPETVDTDFSDNLPSNNHIGTE
                     ETASAKPALPSGLAGFLKASSPETILEKTVAEVQTPEELHDFLKVYETDAVAETAPET
                     PDFNAAADDLSALLQPAEAPSVEENITETVAETP"
BASE COUNT         7595 a         7235 c         6929 g         7029 t
ORIGIN      
        1 tgggcatagg cgacatactg tacggaacgc gctatgccat agacaaagcc gcaatgcgca
       61 acatcgcccc cttacccgcc gagggcaaat tcgccgccat cggcggcttg ggcagcgtgg
      121 cgggctttga aaagaatacg cgtgaagccg ttgaccggtg gatacaggaa aaccccaatg
      181 ccgccgaaac cgtcgaagcc gtcttcaacg ttgccgcagc agccaaagtc gcgaagctgg
      241 caaaggcggc gaaaccgggg aaagctgcgg ttagcgggga tttctcaaaa tcctacacct
      301 gctccttcca cggcagcacc ttggtcaaaa cgacagacgg ctacaaagcc attgcccata
      361 ttcaagccgg cgaccgcgtc ttcgccaaag atgaaaccag tggaaaaacg ggatacaaac
      421 ctgttaccgc ccgatacggc aatccgtacc aggaaaccgt ttacattgaa gtttcagacg
      481 gcctcggtaa aatacaaacc cttgtttcca accgcatcca cccattttat tcggacggaa
      541 aatggattaa agcggaagat ttgaaagcgg gaaacaggct gttatccgaa agcggcaaaa
      601 cccaaactgt ccgcaacatc gttgtcaaac caacaccgct caaagcctac aatctgaccg
      661 ttgccgactg gcatacttac ttcgtcaagg gtgataaggc ggagacggaa ggggtttggg
      721 ttcataatga ttgtccgtat ggtagttctg gcgcgaaagg taaaagtgga agtgagagaa
      781 atactgctaa acgtggaacc ttcagacaag aaacgctaaa aaatgcatgg gatttggcag
      841 cagatggtag ccagcctaat tcaaaaaaat gtcctacatg tggtaaagat gtatatggta
      901 atccccataa caaggagaaa cgtaatacaa aagatggttg ggatggaagt cataatccat
      961 catggagtaa ccgtcaacat agttctgatc gtaaggagca actaaatgat tataataaag
     1021 gagtaagttt agaatgcaga cattgcaatc gatccggtgg caacaaggat gaaaggttta
     1081 agaaaaaatg attatagttg aacatttaga gcattatttg ggtgaaatcg aaagcggtat
     1141 aaaatgcttg gatcgacgtt atcatctcag cgtttccgta ttcccatcac aaccatatac
     1201 aggggtaact actttttcta ctttaggctt gaatcgatat gacttgaact ataagagtag
     1261 gtttgaatta atttttacct gttctgaaga atggaataaa gaaaatattg cagcttttct
     1321 atcaggagta gccgaatatt tgattgacaa tagaaaacct attttaagag gtgaaattat
     1381 tcaattaccc agggtaatta tcgaaggtag caaaatggat gcactgtatg tttccgcccc
     1441 attttatttc gatgatgatt ttcaggtctg ttatggcgaa cactacaata ccgttttccc
     1501 tttgcttgtc ccgttgtata aacaggaagc ggaattggtg gaaaaaaagg gttggaatgc
     1561 ttttgagcag tttttgctgg ataatgaagt tgacaacctt tcggatatga ataggaaacc
     1621 gtttgtttgg taaaggattt tcttgaattt gattaaaggt cgtctgaaat taagcttgtt
     1681 tttcagacga cctttccttc atttgaaaca gaatattgag aactgagttc ttcaaaaatc
     1741 ctacacctgc tccttccacg gcagcacctt ggtcaaaacg gcagacggct acaaagccat
     1801 cgcccgtatc cgagtcggcg accgcgtctt tgccaaggac gaaacaagcg gggtaacggg
     1861 atacaaaccc gttaccgccc gatacggcaa tccgtaccaa gaaaccgttt acattaaagt
     1921 ttcagacggc atcggcaaaa cccaaactgt ccgcaacatc attgtcaaac caacaccgct
     1981 caaagcctac aatctgaccg ttgccgactg gcatacttac tttgtcaagg gcaatcaggc
     2041 ggaaacggaa ggagtgtggg ttcataatga ttgtccgccc tataagagac caaataacgc
     2101 aacgacaaaa gcacaacgag aaagtgtaca aggaaaaccc tgcgttgaat acggtaaatt
     2161 agccgaaaaa atgattgccg accataaaaa accattagtt gtcgagtatt atgaaacagg
     2221 gacaattgac aaatctaaga tgagagcaat tgaatcagta caaccgcaat gtccgacttg
     2281 ttctgcaaaa caagggggga gattaagcca atattcaaaa gaacagaaaa ggaaactgag
     2341 taatgggaat aaaaaatgaa caaataataa tttgtaaaaa atataataca gaaatatatc
     2401 ctatttccga tgtttcaaaa atcggtgttg ctgaaaatgt caaacaggca aacctgtatc
     2461 cgattaacgg gttaaggcac cgtcccaaag gtgatactaa cggttggtat atctgggccg
     2521 gtgaaaactt ctcctatgat aaaaattttt tcctgccctt gcatactttc catttgcaaa
     2581 tatggcgacc cgaaatcatc ccctttttga cacttccgcc cggttaccgt tttttaattg
     2641 gggaaaacgg ttatgaagac gtatggtttg atgagttgct attaaatgat aattgacgaa
     2701 aaatctactt cagatgtact gaaacaatat caagaactag ggtttgattt gacaaaacct
     2761 atgataatag agttttttat aggaggttct caaaaaaatc tgcaatcaat agaaagccaa
     2821 ctcatttctt atcggcaaga ttttcaaatc agtattgagc aaaatgaatt cggagaaatg
     2881 tggacttgct attgttccgt caatatcgtc ctttcccttg aaaacatttt agctattgaa
     2941 actacgttat ttggtattgc acaaaaaaat gattgtaatt atgaaggttt tggttcgtat
     3001 gggaactaac aaaaagtgta ggttgggttg aaaaaaccat attcccatta aagattaggg
     3061 tgtgtctatg gacataaaat tcaatacttt gggggttatc ctgaatgggg tcaatcctga
     3121 agaaaaattc ataaaaatta ttgatgatca ggaaaatacc gggggatttc tgatactgtt
     3181 atcttcaaat gacaaattct catcatttga cagttacgat gactgggtcg aaaacttaga
     3241 aatattaaaa gaatacctgc aagaatctca ttggataata aagtgggtcg gatagtcggc
     3301 aaacgtagtc cgtctgaaac gcttttactt ttcagacgga ctttctatca atgggaacaa
     3361 ggcgttttgt ctcaatccgg tttatgatta ttcgatacag tgatgggaag ctaagaaaaa
     3421 tatgattaat catattgaaa aatatatagg cactatttct catggctcga aaagcgattc
     3481 gggaagtcag tacaaattaa atatcgcagc tattccctct tcccctaaca gggatttaaa
     3541 aacttatatt actttagggt taagcaaaca tgatctgaat tataaaagca gatttgaaat
     3601 actttttgta tgttctttaa aatatgatga aaatcagatt ttcccatttt taagatggct
     3661 ggcagaaacc atcattgaga ataaaaaaat ccttcttcgg ggggcaggtt gtttatttgc
     3721 ccagaagcat tgttgattca acaaaaatgg atgcactgta tgtttccgcc ccattttatt
     3781 tcgatgatga ttttcaggtc tgttatggcg aacactacaa tatcgttttc cctttgcttg
     3841 tccccttgta taaacaggaa gccgaattgg tggaaaagaa aggttggaat gcttttgagc
     3901 agttcttgtt ggataatgaa gttggcaacc tttcggatat gaatcggaaa ccgtttactt
     3961 ggtaaggatt ttcttaaatt taatcaaagt gccgtctgaa accgtgttta attttcaggc
     4021 gacctttcct tcatttgaaa caggatattg ggaactaagt tcttcaaaaa tcctgtctcc
     4081 gtcccttata cgtcggtgct ttagtcaaaa cggcagacgg ctacaaagcc attgcccata
     4141 ttcaagccgg cgaccgcgtc ttcgccaaag atgaaaccag tggaaaaact ggatacaaac
     4201 ctgttaccgc ccgatacggc aatccgtacc aggaaaccgt ttacattgaa atttcagacg
     4261 gcatcggcaa caaccaaacc ctgatttcca accgcatcca tccgttttat tcgggcggca
     4321 aatggattaa agcggaagat ttgaaagcgg gaagcaggct gtttgccgaa agcggcaaaa
     4381 cccaaaccgt ccgcaacatc gttgtcaaac caacaccgct caaagcctac aatctgaccg
     4441 ttgccgattg gcatacctac ttcgtcaagg gcaatcaggc ggaaacggaa ggggtgtggg
     4501 ttcataattc ttgtccgcct aaaagaacag gaagctccaa gaatgaaaaa catggagatg
     4561 gcggtcgaag tcaaatatca gcggaatcaa aaattgctga attaacaaat aaaattattc
     4621 ccggaatgtc caaaaatgaa cgcttaaaga ttaagcaaaa aatcaaaaat attgccaaaa
     4681 atgccaatcg aaaaacaaaa ggagaagagc atggtcgacg cggtcgttaa aactcccgaa
     4741 tttttacctt ttacttctgt aggaatattc cgttttggtg ctgatataac acagtacaaa
     4801 aacatactgg aaacttttat gtatgaacct cctgatgagt ttggaacaga atattatgaa
     4861 tctcccgact caaatttact catttccgtc gaaaaaaaca aaattatttc gatattttgt
     4921 tatcaggagt tatattttat gggcgttaat ataataggtc taaattttga aatttttaaa
     4981 caacttttcc attaccccaa gttaaacgat attgataaat gttatttatc gaatgaatct
     5041 tatccgacat atgtctatga gtttgatgaa ataggagtac aagcttggga agcaaaaggg
     5101 aaaattgtta ctatcattgc cgggggcaaa gacaattatt cgatagaacc ttactacgat
     5161 gaataacatt aatggcaggc ataggttggg ttgaaaactc aacaacaaaa ggtcgtctga
     5221 aaccgtaatc gatttttcag acgacctttc tgtcaatcca aacagattgt cgaatcttaa
     5281 tccaacctgt gcttgctgta cggaacaggc acggtgttca ttcacaagtt aagtcatata
     5341 ttgaatcaaa aggcggcatt gttaaaacag gtttaggaga ttaaacatgg catcatatgt
     5401 ttccataaaa ggatggattg aatgctctga cgatgatata aaaatcatcc aagaaaatat
     5461 caataatttt tggaataatt gtcccttcaa tatcgaagag aaagaatctg caaaaatata
     5521 taaaagcggg tggattttcc cgacaaatag ttttaattgg agcagctata ttttttttgg
     5581 tgcatgcgta aaatcttatt tcattattta ttttgaaaaa tgtattaaaa caataatgga
     5641 attggatatt gaaatatcag gtttttttga attagattat tatgaagata gttataaagt
     5701 aaattggaaa ataaataatg gcaatttaat acaaacgact aattaaatgg acaaaaaatg
     5761 atggctgata aggaacaagt agaaaagatg atttattctg aaaataaaca gagtgttatt
     5821 gacggtatgc tcggtatgac cttcagttca gaggaggatg aaatcccttg gatttctgag
     5881 aaattgacgg agttgtcgaa gcataaagac ttgaatattg ccagattgtc attaacctgt
     5941 ttcggacatc tcgctaggat gcatgaaaat atcggcgatt gcgataaagt gattgcttta
     6001 ttgcgctcaa aacaaggcaa tccggatttt caaggttttg ccgaagatgc gttagacgat
     6061 atttccttct ttatatttaa aaagcatccg taagcaccgg caaggtcaaa tctggcaaat
     6121 gccgtctgaa gcggtattca gctttcagac ggcattttct ccgattggca caggatgcag
     6181 gggcttgccg gcttgatttt gccgaagttg ttggcacgca atttggaggg ggcgacaacc
     6241 tatgaaaaat agatttgcaa aaatcgttaa ggatattttt agttttaccc aatttgtttc
     6301 cgaagcggaa aataaaattc ctaaccaatc ctctcgttat tacgattgct ggcgggaatt
     6361 ggaaatcttg aatgcttccg cattggaaga ttgggaaata tcgggaaagc ctgaaaaatg
     6421 ggagaaatgg acagaaaaat atcagaccga agcgttacag tcagtcaagc atctatttgc
     6481 cgaagaagtt cctgaaaata atatagtttg cgatatttct cattttttgc attttgccga
     6541 ttgggcagaa gccgacattc ccaatcaatc acgtttatat gccgaatatt ggtgggaaat
     6601 ggaaattttc aaaatgtcgg ttttagaaga ttgggaacgc tcgggaaaac cgttagattg
     6661 gaaaaaatgg caaaggcaat atcgatctga agcatttgaa ttagttaaaa aattgaaccg
     6721 aagcaaacaa gagtaacaag ccgcagcctg cataagggtt tttaaagtac ggcagcaacc
     6781 gtagattaaa ttaaaaatca aacaatcaaa gctcgtctga tataatcgat atattctgtt
     6841 aaacgaaaaa taaatatgtt taaaaaattc aaaccggtac tgttgtcatt ttttgcactt
     6901 gtatttgcct tttggctggg aacgggtatt gcctatgaga ttaatccgcg ttggtttttg
     6961 agcgatacag caactgaaat acctgaaaat ccgaatgctt ttgtggcgaa acttgcccgc
     7021 ctgttccgaa atgccgacag ggcggttgtc atcgtgaagg aatcgatgag gacggaggaa
     7081 agtcttgccg gagctgtgga tgacggtccg ttgcagtcgg agaaggatta tctcgcgctc
     7141 gctatccggc tcagtcgttt gaaagaaaag gcgaaatggt ttcacgtaac ggagcaggaa
     7201 catgggaaag aggtttggct ggattaccat atcggcgagg gcggtttggt tgcggtttcg
     7261 ctttcgcaac gctcgccgga agcgtttgtt aatgccgaat atctgtatcg gaacgatcgt
     7321 ccgttttctg taaatgtgta cggcggaacg gctcacgggg aaaattatga aacgacagga
     7381 gaatatcggg ttgtttggca accggacggt tcggtatttg atgcggcggg gcgcgggaaa
     7441 atcggggaag atgtttatga gcattgcctc gggtgttatc agatggccca ggtatatttg
     7501 gcgaaatacc gggatgtcgc gaatgacgag cagaaggttt gggacttccg cgaagagagc
     7561 aaccggattg cgtcggactc gcgcgattct gtgttttatc agaatatgcg ggaattgatg
     7621 ccacggggga tgaaggcgaa cagtcttgtg gtcggctatg atgcggacgg tctgccgcaa
     7681 aaagtctatt ggagtttcga caatgggaaa aaacgccaga gtttcgaata ttatttgaaa
     7741 aacggaaatc tttttattgc acaatcttcg acggtagcat tgaaagcgga tggcgtaacg
     7801 gcggatatac agacctatca tgcgcaacag acgtggtatt tggatggcgg gcggattatc
     7861 cgcgaagaga aacagggaga caaactgcct gattttcctt tgaacttgga aaatttggaa
     7921 aaagaggtga gccgttatgc agaggctgcg gcgagacgtt cgggcgacaa gcgcggcctt
     7981 tcttactgaa cgcaaatgcc gtctgaaacc ggttttcaga cggcattgga gagtcaaacg
     8041 ctttttgcaa tgccgtatat accgaatctg attcgctaca aattgaaaac cacaatccaa
     8101 aaaacggaaa tacacattaa acgggttttt ttaccgaccg tatcatccca taggttttcc
     8161 acaccttttt tcatccataa attcaaacca tttaagtttg catccttacc gacaattgca
     8221 gcagcatcat ccgcataacg gtcacccaaa ttagccaaat aacgccaacc gccataggta
     8281 tcgccacgga aagcataacc acgcaaggtt tggatttgtt ctggtgttaa aacaacttca
     8341 ccctcattca taataagatc ctttaaatta ttttttactt gaattgttta caaaactctg
     8401 taccaattgc tctacttgtt ggtgataacc taccaactcc tgctgtgtga gctgctgctt
     8461 ttcccgaata aacgggtaat actgtaccgt gaaaccgtta atacaggaac ctatatcagt
     8521 actgtaatcg ttctctccat acctatgata gttctgatga gtaataatcg ccggattttc
     8581 tcctttctta atatactgta gttcatgaat atatgggttt tcaggaatcg accttttatc
     8641 ttcataagct tcatattcag gatagacaga cgggattttt ttaaatccca aatcaagcaa
     8701 cgattttcct aagtcttgat tcaaatgaca cgcatctttt cggataacaa tatcgtccac
     8761 gctatccaaa ccatcgcgta gaaattgaat aaagtgtgct ttgttctgct ggttgtatgg
     8821 ttcgagcacc cagccttggt cggttttttt gtaatagacg ctaatctcta tcaaattaat
     8881 ggagtaagca tcctgcccgg gcgtgtcgga aagccatttt gtggcattgt cttgcgtata
     8941 ccgtgcaaga tagccataag ggataaccat tttggtgccc tgaaaatcaa atgtaaccgt
     9001 atgttcaaat cctgtcattg gctcgggatt gttgaaactg gtttgttcat taacaggttc
     9061 acatgaggtc atagtaaaaa cactcccaat aaacaaaata aaaagttgat aaatggtagt
     9121 agcatatggt ctcataattt caagcctaga aattagttaa agaatatatt atggtagata
     9181 ctaactcttt aagttgacat attgtgcgat atcgagggct gtaccgtatt ttcccatcgt
     9241 attttcccat cgtattttcc catcgtattt tcccatcgta ttttcccatc gtattttccc
     9301 atcgtatttt cccatcgtat tttcccgttc tttatatgag cggcaggcat cctcataaaa
     9361 gaagggatac cgataaggaa aaatacccca gcataaaaaa tatactgatt aatcaataca
     9421 aatccaacaa acagttcatc gtattctttt ctgttggcgt ataaaaaata ctgatatatg
     9481 ccttcacttt catagggcga cgctttatgt accgtcctgt gtgttgaaac gtcggttttc
     9541 cttaccgcct gccccgcttt tccctgattg ctgccaactg ccgtccgaac ctgaaccgtc
     9601 aggtttcaga cggcattttt tctgcctgtt cccacaattt ccgtttttgt gcggcggtca
     9661 gttttttgac ggcgatttcc tgcctgagtg ccgtgccttt atccatcccg cctgcaacga
     9721 tacgcatcgc caccggtttg aatacgcggg tatatttcgc gcctttaccg gttgtgtggg
     9781 cggcaagccg ctgttgcgga ttcgggctga tgccgcaata gaacgcgctg ttttcacaca
     9841 atatcaggta aacgctccag tttgacgcgt tcatctgcct gcatcctgag ttttctcccc
     9901 gttccaaggc gcaactttca gcagcagcag catacccggt agggcgagtg cgaaacacag
     9961 ttggaaaaac ggtacataac cgagccattc aatcagataa ccggcaaagg aattgatgac
    10021 cgtgcgcggg acggcggaca ggctggtaaa cagcgcaagc tgcgttgcgg taaatgcggg
    10081 attggtttca cgcgccatat acgatacgaa cgccgccgtc cccaacccca cgccgaccgc
    10141 ttccgcgccg ataactgccg ccagcatcag cctctcgcct gtgccgaccg tgtcgaaagg
    10201 tccgaaccct gccagccata caaaccccaa aacggttaca gcctgcaccg cgccgaatag
    10261 ccacaaggct ttgtttacgc cgattttcag catccacaca ccgcccaaga tacctgccgc
    10321 cactgccggc cacagtcctg catttttcgc aatcaaaccg atgtctgtct tgctgaaacc
    10381 catatccaga taaaacggcg ttgccaacgc ggttgccata ctgtcgccga gtttgtaaag
    10441 gaagataaac agcagcacgc acaccgccga agcgatgccc ttgcgcgtaa aaaattcttt
    10501 aaacggctct accacggtct gcttcaacgt tttaggaacg gcaggaggca acacgggttc
    10561 gcgcgcaaga aacagcgtca tcagaaggcc gggcagcata aataatgaag tgataacaaa
    10621 tacttctgac cacggcatcc tgtctgccaa caccaaactc aatgaaccgg gaatcagggc
    10681 ggcaatccgg taggcgttca catgaaccga gttgcccaaa cccaattctt cgtctgacaa
    10741 aatctcgcgc ctgaacgcat ccaatacaat atcctgactg gcggaaaaaa aagcgacaag
    10801 caccgacaag ccggcaatca gcggcagatg attacggggg tttaaaaagg cataggccgc
    10861 caaagccgcc agcaaccctg cctgcgtcag cagcatccac ccgcgccgcc gtcccaaaac
    10921 gggcagcctg accgcgtcca tcagcggcga ccacaaaaat ttccaagtaa acggcagacc
    10981 gattaacgcc atcagcccga tgctcttcaa atccacctgc tcgctgcgca accacgccgg
    11041 aatcaggtta atcagaaagt acagcggcag ccccgaggta aaccccgtaa aaatacagac
    11101 aagcatattt cgggaaaaga tttgcccgat aaaacctgat ttcgatgcag tcatcgttcg
    11161 tgcagcccaa aaaaaacaaa agcctattgt agcaaatatc gcccgcctgc tgaaaaatgc
    11221 cgccccgcgc cgccagtttg ccgttcaaat gcaaaatgcc gtctgaaact gtgttcagac
    11281 ggcattgctt ttctgaaaac cagacgtgct tacaggctgt aatacatttc aaattccagc
    11341 gggtgcggcg ccatgcggat gcggcgtacg tcttcttctt tgaaagcgat gtagctgtcg
    11401 atccagtctt tgctgaacac gccgccgcgc aggaggaatt cgtggtcggc tttgagggcg
    11461 gccagtgctt cttccaaaga agcgcaaacg gtcggcacca atgcatcttc ttccggcggc
    11521 agatcgtaca ggtttttatc ggcagggtcg cccggatgga ttttgttttg aatgccgtcc
    11581 aaaccggcca tcaacagggc ggcaaatgcc aaatacgggt tggcggtcgg atcggggaaa
    11641 cgcgcttcga tgcggcgcgc cttgctgctg ttcacggacg gaatgcggat ggaagcggaa
    11701 cggtttttgg cggagtatgc cagtttggtc ggcgcttcaa agtgcggcac gaggcgtttg
    11761 taggagttgg tggacggatt ggtaatcgcg ttcaaggctt tggcgtgttt gatgatgccg
    11821 ccgatgtagt agagcgcggt gtcgctcaag ccggcatagc cgtcgcctgc gaacaggttt
    11881 tgaccgtctt tccaaatgga ttggtgaacg tgcataccgc tgccgttgtc gcccataatc
    11941 ggtttgggca taaaggtggc tgttttgccg aagttgtggg caacgttttg aatcacatat
    12001 ttcatgtctt gggtttggtc ggcgcgtttg accaaggtgg cgaaacgcgt gccgatttcc
    12061 atttggctgc cggtaccgac ttcactgtgg tggacttcga cttcgatgcc gagttcttcc
    12121 aaaatgttta ccatcgcgga gcgcaggtct tgtcccgcgt caatcggagc aacgggcgcg
    12181 taaccgcctt tgacggcagg gcggtggccg gtgttttgac cgtccatatg cagaccgctg
    12241 gcccatgcgc cgctttcgga cgtgatttcg taacgggttt tgtgcatatc ggtttcaaat
    12301 tctacgccgt cgaagacgaa gaattcgggt tcggggccga agtatgccgt gtcgccgatg
    12361 ccggaagatt tcaaataggc ctcggcgcgg cgtgcgatgg agcgcgggtc gcggtcgtaa
    12421 ccctgaccgt cggcagggtc gatgacgtcg caggtaatga cgacggtggt atcgtcgtag
    12481 aaaggatcga cgaaggctgt agacgcatcg ggacgcaact gcatatctga agcctgaata
    12541 cctttccaac cgccgatgga cgaaccgtca aacgcctgtc cgttttcaaa ccactcttcg
    12601 gggtcttcca acacgatgcg cgcaggcacg gtaaagtggt gctgcttgcc tttggtatcg
    12661 gtaaagcgca aatcgacaaa gcgggcttcg ctttcttcaa tcaattttac ggcgttttta
    12721 atagacattt ttcagctcct ggaaaaggga aatagcaaaa tatgcggaac gcggcagatt
    12781 agctcaaagc cagcatgatt ctaccacgtc catgccgtct gaaactggcc ctgcgtcaaa
    12841 ttccaagctg taaaatatag tttcagacgg caatattgtc aacaatatgc ttcggtttgc
    12901 ccgttcccaa gcagaatgcc cgccctttta tccgaatgcc gacaaaacag atgccgtctg
    12961 aaagcagttc agacggcatc tgttgtttgg tttgaaaacg gtcagcgagg atagccgttg
    13021 cagacaaatt tgtactgagc ctctccaagc gtaccgggaa taacataatt ccattcggca
    13081 taggatggtg tataagaagc gatagagttt cccgatttgg aataccacgt tgccgtcaat
    13141 atcctaaaac gtttaccact gcaatccatt tcttccaacg ttttagctgt attaaaaaat
    13201 tttccattgc catacttttg cggcttggcg aaccttgttt caaaccatgc actgttgtcc
    13261 gtacggctgt ctgcatcacc aaacgtggca gtattactat ctaccaatac ccaatccgcc
    13321 gccaccgccg gcgttgccaa ccccaatgca aatactgcca agcacaacat ctttttcatg
    13381 tccgaccctc tcgtccttgt caacaaaata aagttaaatt gtaaaaaaaa acgacttttt
    13441 tcaagagaaa aatattccgt catgctatct gacaaccgtt tcagacggca tcgtttccat
    13501 ctgtacgccg ccgttataaa atagccttcc cgtcttttgg aacacgacca tgaccgccct
    13561 cccccgttac tccgttttcg gcaaccccgt tgcacacagt aaatcgccgc aaatccatca
    13621 gcaatttgcc cttcaggaag gcgttgacat tgaatacgaa cgcatttgcg ccgacatcgg
    13681 cggttttgca caggcggttt cgacattttt tgaaacaggc ggttgcgggg caaacgttac
    13741 cgtaccgttc aaacaggaag cgtttcatct ggcggacgag cattccgaac gcgcattggc
    13801 ggcaggtgcg gtcaatacgc tgattccgtt gaaaaacggc aagctgcgtg gcgacaacac
    13861 cgacggtttg ggtttggtcg gcgatatagt gaaggtgcaa cacacagaaa ttgaaggcaa
    13921 aaatatccta ttgctcggcg cgggcggcgc ggtgcgcggc gtgattccgg ttttgaaaga
    13981 acaccgtcct gcccgtatcg tcattgccaa ccgtacccgc gccaaagccg aggaattgtc
    14041 gcggcttttc ggcattgaag ccgtcccgat ggcggatttg aacggcggtt ttgatatcat
    14101 catcaacggc acgtcgggcg gtctaaacgg tcagattccc gatattccgc ccgatatttt
    14161 tcaaaactgc gcgcttgcct acgatatggt gtacggctgc gcggcaaaac cgtttttaga
    14221 ttttgcacga caatcgggtg cgaaaaaaac tgccgacgga ctgggtatgc tagtcggtca
    14281 agcggcggct tcctacgccc tctggcgcgg atttacgccc gatatccgcc ctgttatcga
    14341 atacatgaaa gccctataaa catgttccgc atcatcaaat ggctgattgc cctgcccgtc
    14401 ggcatcttta tctttttcaa tgcctatgtg tacggcaaca tcattaccta ccgcgccgtc
    14461 gcgccccatc ggactgcctt tatgtcgatg cggatgaagc agtttgaaca ggaaggtcgc
    14521 gatgtcgcac tggattaccg ctggatgccc tacaaacgca tttccaccaa cctgaaaaaa
    14581 gccctgattg cttccgaaga tgcccgtttc gccgggcacg gcggcttcga ttggggcggc
    14641 attcaaaacg ccatcaggcg caaccggaac agcggcaaag tgaaggcggg cggctcgacc
    14701 atcagccagc agcttgccaa aaacctgttt ttaaacgaaa gccgcagcta tatccgcaaa
    14761 ggcgaagaag cggcgattac cgcgatgatg gaagccgtta ccgacaaaga caggattttt
    14821 gaactgtatt taaactcaat cgaatggcac tacggcgttt tcggcgcgga agccgcgtcc
    14881 cggtattttt atcaaatacc cgccgccaag ctgaccaaac agcaggcggc aaaactgacg
    14941 gcgcgcgtcc ccgccccgct ctactacgcc gaccatccga aaagcaaacg gctccgcaac
    15001 aaaaccaata tcgtgctcaa acgcatgggt tcggcagagt tgcctgaaag cgacacggac
    15061 tgagtgccgc cgcaatataa aatgccgtct gaaatatcat tcagacggca tatgctttct
    15121 gcctttaccc tcttctccaa aacgcaacgg ggcggtcatt atcatttttg cccttctaca
    15181 taacggtcag acacaaaacg tgtccgacac ggacaacttc ccaaaaacgg caggcaaaaa
    15241 aaataaccgc ttcaaggcgg ttttttggtg gccagaggcg gaatcgaacc gccgacacac
    15301 ggattttcaa tccgttgctc taccaactga gctatctggc ctttgtctgt ctcgttaaga
    15361 gatgcgtatt aaaccaaatt acgggacgac atgcaagcat atcgggcaat attctttgaa
    15421 aacaggttta agtcaatgtt ttaaatttga attaatttat gtaaaaacaa cataaatccg
    15481 tatttttgcc gcctgcccga cgatattagg tcgcccgtct gaaaaatatt ttcaatattt
    15541 gaagttctta cccagataaa cagaacgaac ctgttcgttt ccgaccaaat catcaggttt
    15601 tcccgatgcc aacaccgtgc cgtctgaaat aatgtaggcc cgatcgcaga tgctgagggt
    15661 ttcgcgtacg ttgtggtcgg taatcagtac gccgataccg cgcgatttga ggaaaccgat
    15721 gattttctgg atgtcgatga cggcaatcgg atcgacgccg gcaaaaggtt cgtccaacaa
    15781 aataaaatgc ggtttcatgg cgagtacgcg ggcgatttcg acgcgccgcc gttcgccgcc
    15841 ggacagcgac ggcgcggggc tgcggcgtaa gtgtccgata ttgaggtcgg cgagcagttt
    15901 ttcgatttcc ctgtcgattt gatttttatc tttggttctg atttccaaga tggcgcggat
    15961 gttttgttcg acggtcattt tgcggaatat cgaggcttcc tgcggcaggt agccgacacc
    16021 gaggcgggcg cgttcgtgta tgggcaggtg gcgcaattct tgtccgtcta gggttacgct
    16081 gcctgcgtcg gcggcgatga gtccgacaat catgtagaag ctggtggttt tacccgcacc
    16141 gttgggcccg agcagtccga tgacttcgcc gctttcgatt tcgagggaga agcttttaac
    16201 gacttggcgt tttttgaaac ttttttgcag gttttgaaca acaaggcggc tgacgtttgc
    16261 actcataaat atctcttcaa tatcttcggt cggcatatgc cgtccgaacc gggtttatat
    16321 ttcagacggc attttgggat tattcggatt tttgcgtact cgaaggctgg atgatgacgc
    16381 tgaccctgcc ggatttggaa gcggatttcg caccggattt tgtactgccg ctgatggtat
    16441 agacttcagt tttggtgttg taggtaatca ccgcaccttc ggcgacatcg ccgccgcgct
    16501 gtactttggc attgccggtc agaacaacgg tgcttcctgc ggaggaatag gtaacgttgt
    16561 ttgcctgtcc gcgcaccgtg cctttgccgc cgtccaatgt ctggctgaag cggactggcg
    16621 aaccttccgc cctcacggat tcgccgcctt tgccgccgcg tgtaacattg acgcgggcgg
    16681 cggaaatatt gagcgtaccc tgtctgatga cgacgtttcc gctgaatgtg gtgctttggt
    16741 tggcttgatc gagcgaacct tggtcggcct caatctgaat aggctgcctg ctgtcgcttt
    16801 gaagggcaaa agcgggggac gccgaaaaaa atgctattaa aacaaatagc ttacatatct
    16861 tttgtatcat aaatcgtggc tttcacttta gatgagaagt tcaacatgcc tgttttgtgg
    16921 tcgtaagtca tgccgcccgc ctgaccgtgc gatgcaccat attggaaact gacaggcgta
    16981 tcggtttggg catattgaga ttcggtatcg acgtgcagct tttcggcttc aactttaccc
    17041 gcctgccgtt tgccgtcggc ggttttggtc agcacaacgt tgtttttaaa aagaacctgt
    17101 ttgttttcgg tatggtaaac ggcttcatcg ctgccgactt cgtacaacaa cctgccttct
    17161 tggaagaaca cgagatgcgg tgagtcgaaa tgaatgtcgc tgctttcggg aaactgtttc
    17221 gcgcccttcg aactcaaatg ttctttcaag tatccctgtt cgtcaaaccg cctgccatcc
    17281 aatccgtcca ttgtgtattg cggttcgtcg ggattgagcc tgacttcttc aatctcgact
    17341 tcgctgatgc gtcccaacca tgccgacagg ctgcccaagg caaccgccaa tatcaatggg
    17401 aacgcaattc cgtaccgcca tcttactttc atttgatgta ctcgttcaaa gccgcgccca
    17461 aagtcccttg cgcctgcata atcaggtcgc acacttcgcg caccgcgccc gcaccgccgg
    17521 ggcgtttggc aatataggcg gcgtgttgca gcgtaaacca atgcgcgtcg gggacggcaa
    17581 ccggcaatcc gcagcgtacc ataacgggca aatcgaccac gtcgtcgccg acaaaggcac
    17641 actcggcttc ttccacgccg acctgagcgc gcagctcttc atacgcggcg cgtttgtccg
    17701 aaatgccttt gaagtagtaa tttatgccca actgtttgac gcggatgccg acggagggcg
    17761 cgtcccgacc tgtgataatc gcagtctgca cgccgcttgc ctgaagcatt ttcagaccgt
    17821 gtccgtccag tgtgtgaaac gatttgattt cttcgccgtt atcgcggata aagatgcgcc
    17881 cgtcggtcaa aacgccgtcc acatccagga tcaacagttt gattttggcg gcgcgcgcct
    17941 gtaattcggg agaaattgcc tgcatgggat ttccttgttt tcagacggca tatcttacca
    18001 caaatgccgt ctgaaacggc tgcaacgcct tatatagtgg attaacaaaa atcaggacaa
    18061 ggcgacgaag ccgcagacag tacagatagt acggaaccga ttcacttggt gcttcagcac
    18121 cttagagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtt tttgttaatc
    18181 cactatacaa tccgcgccgc caaaaggtcg tgcatattca gcgcgccaat cagcacgccg
    18241 tctgcatcgg taaccagaag cccgttcaca tggtttgcct gcatgacttt cagggcttcg
    18301 gtggcgagac gttcggcgga gatggtttta ggatgcgtat gcatgacttc gtctatcgaa
    18361 agaccggtaa aattgtcgca ttcttgaaac aggcggcgca aatcgccgtc ggtgaatacg
    18421 cctttcagac ggcattgccc gtccgttacc gccaacatgc ccagcccttt ttcactcatg
    18481 ctgacgatgg cttctttcaa gggcgtgccg agtcggacgg caggcaggcc gccgcctttg
    18541 tgcataatgt cggcaacgcg caaaagtagg cgtttgccga ggctgccggc aggatggctc
    18601 aaggcgaaat cgtcgggcgt gaacgcgcgt gcgcgcagca ggacgaccgc caacgcatcg
    18661 cccaaagcca tgacggcggt ggtgctggtg gtcggggcaa gccccagcgg gcaggcttct
    18721 ttggaaaccg acgccgtgat gtggatgtcg gcatggcgcg ccatggttga atcggggcgg
    18781 gcggtgatgc agacaagcgt gatgtctttg cgtttgagtg cggggatgat ggcggcgatt
    18841 tcgtcgcttt cgccggaatt ggaaatcgcg acgaccacgt cgttgtccac aatcataccc
    18901 aaatcgccgt gtgccgcttc cgcagggtgg acgaaaaacg caggcgtgcc ggtcgaggcc
    18961 atagttgccg ccattttgcg cccgatatgt cccgacttgc ccatgcccgt gataacgacc
    19021 ctgcccttgc agtgcaacaa cgcgtctgcc gcaaggacga agtttttgtc caattccgct
    19081 gcaatttcgc gcaagccttc cgcttcggcg tgcaacactt cgcgtgccca gtcgagatat
    19141 tttccgtttt ctgccattgc catccgcctt tgctgtaata aaactaccca aaatttccga
    19201 atcggggtag cataagccct caaaccccgc ctatccatcc aaaggaatca gagatgacca
    19261 ttttatcgga cgttaaagca ttaggacaac aaatctggct ggacaacctt tcccgctcgc
    19321 tagtgcaaag cggcgaattg gcgcaaatgt tgaaacaggg cgtgtgcggc gtaacttcca
    19381 atcccgccat cttccaaaaa gccttcgccg gcgacgcgct ttacgccgat gaaatcgccg
    19441 ccctcaagca gcaaaacctc agccccaaac aacgctacga aacaatggcg gttgccgatg
    19501 tgcgtgccgc ctgcgacgtt tgccttgccg aacacgaatc caccggcggc aaaaccggct
    19561 tcgtcagcct cgaagtttcg cccgaattgt ccaaagacgc gcaaggcacg gttgaagaag
    19621 cgcgccgcct ctacgccgcc atcgggtgca aaaacgcgat gattaaagtg cctgccaccg
    19681 acgcaggcat cgatgcgctc gaaaccctcg tttcagacgg catcagcgtg aacctgaccc
    19741 tgctgttctc acgcgcccaa accctcaaag cctacgccgc ctacgcgcgc ggcatcgcca
    19801 aacgcttagc agccggacaa agcgttgccc atatccaagt tgtcgccagc ttcttcatct
    19861 cccgtgtgga cggcgcgctg gacacaacac tgcccgacca cctcaaaggc aaaatcgcca
    19921 tcgcccttgc caaagccgcc taccaagact gggcgcaata cttcggcagc cccgaatttg
    19981 ccgcgctgga aaccaaaggc gcaaaccgcg tgcagctttt atgggcatct accggcgtga
    20041 aaaacccggc ctatcccgac acgctctacg ttgacagcct gatcggcgcg cacaccgtca
    20101 acaccgttcc cgatgccacg ctcaaagcct ttatcgacca cggcacagcc aaagccacgc
    20161 tgaccgaagg cgtagaagaa gcacaagcgc agctcgccga aaccgccgcg ctcggcatcg
    20221 atgtcgaaac cttggcaacg cgtttgcagg aagacggttt gaaacagttt gaagaagcgt
    20281 ttgaaaaact gctcgcgcct ttggcttagg cattttcccg atgccgtctg aaaggctttc
    20341 agacggcatc gcccgtttga ccggacacag tgcggcatat caaggattca ccctaaaaat
    20401 attaatgggc aagaagttaa tgcacgacat tacccactaa cacaaaagga aatagaatca
    20461 tggcaaaagc aatcgaaatc atttcaccca atgaaattta ttcagatctg atttttaagg
    20521 atccggtacc tccccatact attgaatata aaatgattaa cctagaattg aaagccatca
    20581 atctttacga ttgtgccttt gaagatttcg ttcccgaaat caaagacaat ttttgtgtgg
    20641 gaattgattt agacatagga actgatgata gtgatgctgc agatatattt tctgttcgca
    20701 tatgctctcc taaatggatt ttgcataatt gtttccaaaa tcaaagggtt aaatggggag
    20761 cgggtatgat gattatgaat gaatttaatc attctgttat taaatcggaa attgagaaaa
    20821 ttcttaaaga atgttcaaaa gaaacttggg aaaagtcatt gacttattta cttcgttttt
    20881 tttcgtggga atttgaagat tatcagtgct aattaaatca aaaacagcga aaccaaaact
    20941 ttttcgctgt tttatttttc cataaacttt tcaaaatata cagaattgac gaaactggtt
    21001 agtagaaatg caagtaatga gagaattgca gatagagcct ttgatttttt ctcgcctgct
    21061 cttatggatg gttcagctac cgaagagcaa tataatgccc tatatgattt aaccttgctt
    21121 gaagagcccg ggatggaact gaataaagat gagataatgg ctttgattaa tagtcttaaa
    21181 taaaggggct gtaccagata tgtacacagg acgttttgcc cccagcccga ccgggctgct
    21241 ccacatcggc tcgctgctga ccgccgtcgc ttcctatgcc gatgcgcgcg cgcacggcgg
    21301 caaatggctg atccgcatgg aagacctcga tccgccgcgc gaaatgccgg gggcggcaag
    21361 ccatatcctg cacacgcttg aggcattcgg attcgagtgg gacggagaag tcgcctatca
    21421 gagccgccgt tacgccctgt atgaagaaac cctatgccgc ctgaaaaccg ccggactggt
    21481 ctatccctgc cattgcagcc gcaaagactg gcaggccggg gcaaggcggg gcgcagacgg
    21541 gttcgtctat aacggacgtt gccgccaccc cggccaacgc cctgcactgc aaggcaaaca
    21601 gccggcgtgg cgcatccgcg tccccgaccg cattatcggt ttttcagacg gcatcgtcgg
    21661 cggttacgcc caaaacctcg cccgcgacat cggcgatttc gtcctgcttc gtgcagacgg
    21721 ttactgggca taccagctcg ccgtcgttgc cgacgatgcc gaacagggcg ttacccacat
    21781 cgtccgcgga caagacctgc tcgtttccac gccgcgccaa atctatttgc agcagtgttt
    21841 gggcgttccg acaccgcaat atgcccacct gccgctgctg accaacgcac aagggcagaa
    21901 gtggtcgaaa cagacgctcg cccccgcatt ggatttaaac cgccgcgaac aactcctccg
    21961 ccaagtgttc cgttacctca agctgcccga agcaccggaa accgaccgcc ctgccgaact
    22021 gctcgactgg gcggtggcac actgggatat ggacaaagtg ccgaaacacg ccgttaccgc
    22081 cccctaaaca ccgcccgaca aacgcacaaa tgccgtctga agccttcaga cggcatttca
    22141 cacatacagc cccgccctat tcgcgcatat ttgcccgttc gacctctttc cacgcttcga
    22201 gaatcaggct gccgtcgttg tctttcaaga gcgttgcgtc ggaaagcata cgccgccaag
    22261 tccgcgcgcc tttcagaccg tgcatcagcc caaggctgtg gcggacgatg tgacgcaaga
    22321 ttgtgccgcg tccggcttgg atttgggctt ggctgtatgt gtagagacgc tgcaccaaat
    22381 cggcgtattc aatcgggctg cgggtatcgc cgtaaaacag cctgtcccat tcgcgcatca
    22441 ccatcgggtt gtggtacgcc tcgcgcccga ccatcacgcc gtcaacgtgt tgcaggtgtc
    22501 cggcgattgc ttcgttggtg gtgatgccgc cgttgatgat gatttccagc ccgggaaact
    22561 cctgcttgag gcggtaaacg taatcgtatt tcaacggggg aacgtcgcgg ttttctttgg
    22621 gggaaagacc gtccagccaa gcgttgcggg cgtggacgat aaaggttttg caggcggttt
    22681 tgtcgcgcag cgtgccgacg aaatcggcaa cggtttggta ttcggtctgc ctgtccacac
    22741 cgatgcggtg tttgacggta acgggaatct tgaccgcatc ctgcatggcg ttgaggcagt
    22801 cggcaaccag cccgacttcg ttcatcagac acgcgccgaa cgagcctttc tgcacgcgcg
    22861 gactggggca gccgcagttg aggttgacct cgttgtaacc gtatgcctcg gcggctttgg
    22921 cggctttcgc caaatcggac ggatcgctgc cgcccagttg cagggcgacg ggctgctcgc
    22981 cttcgttgaa catcaaaaag cggtctttgt cgccgtaaac aatcgcaccg gcattgacca
    23041 tttcgctgta cagccaagta tttcgggtaa tctggcgggc aaggtaacgg tagtgcctgt
    23101 ccgtccagtc gagcatgggg gcgacggaca ggtggcgcgg gggaagggtg tgtgtattgt
    23161 cgttcattgt cgatatttta tcattgcgga ttcaatttca atccggaaca accttcattt
    23221 ttggggaaat ccgcgtcatg aaagagtcca gcagtatggt taccggccca tcgttgcaga
    23281 gcgacacctg catatgcgtg cggaaacgcc ctgtttcgac atgaatcccg tgtccgcgca
    23341 acagttccgc cgttcgcagg taaagctgct gcgcctgttc tgcaggtgcg gcttgggaaa
    23401 acgaaggccg ccgcccgctt gccgcgtcgg cataaagcgt aaactgcgac accagcagca
    23461 ccgcgccgcc gacatctttc aaagacaggt tcagcttgcc cgcttcgtct tcaaacacgc
    23521 gcaaatgggc gattttgtcg gcgatatagc gtgcatcttt ttctgtgtcg ctatgcgtta
    23581 cgccgagtaa cacgacaaac ccgccgtcga ttttgccaca ggtttccgtg ccggcttcgg
    23641 acacgacatc cacctttgca cctaccgttt tctgtatgac cgcacgcata agccgtcctc
    23701 cttacctgaa cgtgcaatgc cgtctgaaag gcttcagacg gcatcggtat tggaaatgcc
    23761 attacggttt gacaccgttt tcttccaaaa gggcatcgat tttcaagcgg gctttttcct
    23821 gtctcaaacc ctgctccaac tggtttctga ccaactcgaa aggctgcgcg tcggggtttt
    23881 tcccgacctc gctgagtttg aacagataat agcgttcgcc caatttgacc ggatcgcggg
    23941 taacgtcgcc ccgattcatc gcggcaaact gcgaagccag cggctcggga agctgctgcg
    24001 ccataatgaa accgtcaaaa gcctgctcgt cgttcggata acgcttcatc agcccttcaa
    24061 aagacagccc tttgagcagg agctgctgcg cctgacgcgc ctcctcttcg gttgcgaagc
    24121 tgacctgctg caatttgatc atgcggattt gctgttcgta aaacttgtgc agctcgtctt
    24181 cggaaaccgt ttccgaacgt tccagaaaac ggacgtactc ctcggcataa aaagacgctt
    24241 cggcgatttt aaagcggttt tggacatcct tatccttatc caaaccttcc ttcaatgccc
    24301 tgtttttcaa aacttccaaa gtttgtagcc ggcggacggc atcgtttcgg attgcctgcc
    24361 cgtccggttt ttgggactgc tccgcatgcc ggtctgcctg ctgcatgatc tgcgccacca
    24421 gcgtatccac caaagccggg tcgatttcgg gtgctttggc tgccgcaaaa cctgccaaca
    24481 ttgcagcaat aactgcggca gcggtttttt tctgtttcat ggtattccct gtcctgcgcg
    24541 gcggcaaaac gcctgtctga acgtcttgcc gccatatccc gttttcagaa ttattttgca
    24601 ggtttgatgt ttgccttgcc caacagtgca ccgacggcac ggtcaatccg ttccgcctga
    24661 aggttgcccg caatctgtcc tttcatttca tcaaaagaag gcacttttac ctcgcggctg
    24721 tcgttgacat aataaacgcc gtagaaatcg ccgtttttca gcggcgttgc cgtaaattcg
    24781 ccttttttca agtccttaat tgcctgataa agcggcggaa caccctgttc caaatctttc
    24841 agcggcacat atccgaccgg cgcaccggtc tgtttggtac ggtcgttgag ggaatattgt
    24901 ttcaagacgg catcgaaacc ttttttcgcc ttcaagtcgg caaccgcttt ttttgcattt
    24961 tcttccttgt cggtcaggat ttcgcccaac tggacttcct gcgtaccttt gtaaaaaccg
    25021 ctgatattgt catatgcggc ttttacttcc tgctcggaaa ccggttgggt tttggcgata
    25081 tgcaatgcgt atgcctcgcc gttcaagcca tattttaccg cctgccaaac ggttttgaag
    25141 gacggtttct tgtcgtcgcc cgactttttc gcttcggcac gcaatttggc aagcgcattt
    25201 ttaaactctg ccgaccggtc gagtttcagg cgtttcactt cctgtgcgac cacggtattg
    25261 accacttcgt tttccagcag ggattggcgc agttgcggcg tgtcttcggc acggctgttt
    25321 tccgcacgga atgcggcaac ctgcgcatcg atgacggaac tgtcgatttt ctgaccgttg
    25381 acggttgcca gcgtttgggc aaacaggctg ccggaacagg caagcagtgc aacggaagtc
    25441 aggatttttg ctttcataat cttctctctt taaattttaa attgaaatgt tcgggcagac
    25501 ttcattcagt ccgccgtatg gaaatactcg tcaggggtag ccgcccggat gccgagcgcg
    25561 tgaatcatgc cgcctgaaaa caaatctttg agcagcgatt tgaccgtttt ctggcggttc
    25621 aggcggctta cgccttcaaa acggccgcta acgaccaaaa cggcataatg tccgccgccg
    25681 gtattgcccg cgtgtccttt gtgcagatgg ctctcatcgc cgatttccaa caccagcgga
    25741 tcgagcgtct gcaggcgttc gcggatcaaa tcgacggccg gcattatttg aacaccgctt
    25801 taaacggctt gatcagcact tcgctgtaca cgcctgcatg aacataggga tcgtcttccg
    25861 cccaagcctg cgccgcatcc aaagactcga actgcgccac aatcaaactg cccgaaacgc
    25921 ggttggagtc ctccggcaaa ggattcgggc ctgccgtcaa cagccggcct tccgacttca
    25981 gcgtctccag ccgtttgagg tgttcgggac gtgccgccat acgcgcctcg tgcacatcct
    26041 ccccgtctgt tgccagcaac ataaaatatt ccacagtcaa tcctcctttt tcagacaggt
    26101 actcagataa ataccctgaa taataaagaa aacaagcatc agtgcagtcg agccgaacat
    26161 tttatagttg acccattgcg actcgaaccg ggtaaacaca aaccagttgg cgatacccat
    26221 aaaaatcagg aaaccgaccc acatatacgt caatttcgcc catacggcat ccggaagctg
    26281 aatctccctg ccgatactcg ccttcaagcc gtttttaccg gcgaggtggc tgccccacag
    26341 gaacagcgcg cccagccaaa acaaaacgct cggcttccac ataatgaagc ggctgtcgcc
    26401 caaaacaatg gttgcgccgc cgaataccac aatcagcacc aatccgaccc actgcatcgt
    26461 atccagcttt ttatatttcc aatacagaaa agccgcctga accacaccgg caaccaatgc
    26521 gaccgccgtt gcggcaatca tgtttttggt aacggtatag gtggcgaaaa acaggatgac
    26581 ggacaaaagg tcgctgacga atttcatatc caaatgagaa agcaaatcaa ataaacgcta
    26641 atcatacagg cagaaccctg tttttgcacc tgcaaaacca acaaattttg ttttgattga
    26701 tgcaaatatg cacagatgtt tttgaaaaaa gatggagata tgtcataatt tgtaaaaacg
    26761 gcgaatgatt gccgtttaaa atgtggcggc ggtcggtaca ttcacatatt aaatgcccgc
    26821 cggccgactg ccccgccgat gcccgatgat gacgaaattt acagactgta cgcggtcaaa
    26881 ccgtattcag ccgccaaccc acaggggata catcttgaaa aacaacagac aaatcaaact
    26941 gattgccgcc tccgtcgcag ttgccgcatc ctttcaggca catgcgggac tgggcggact
    27001 gaatatccag tccaaccttg acgaaccctt ttccggcagc attaccgtaa ccggcgaaga
    27061 agccaaagcc ctgctaggcg gcggcagcgt taccgtttcc gaaaaaggcc tgaccgccaa
    27121 agtccacaag ttgggcgaca aagccgtcat tgccgtttct tccgcacagg cagtccgcga
    27181 tcccgtcctg gtgttccgca tcggcgcagg cgcgcaggta cgcgaataca ccgccatcct
    27241 cgatcctgtc ggctactcgc ccaaaaccaa atctgcactt tcagacggca agacacaccg
    27301 caaaaccgct ccgacagcag agtcccaaga aaatcaaaac gccaaagccc tccgcaaaac
    27361 cgataaaaaa gacagcgcga acgcagccgt caaaccggcg tacaacggca aaacccatac
    27421 cgtccgcaaa ggcgaaacgg tcaaacagat tgccgccgcc atccgcccga aacacctgac
    27481 gctcgaacag gttgccgatg cgctgctgaa ggcaaaccca aatgtttccg cacacggcag
    27541 actgcgtgcg ggcagcgtgc ttcacattcc gaatctgaac aggatcaaag cggaacaacc
    27601 caaaccgcaa acggcgaaac ccaaagccga aaccgcatcc atgccgtccg aaccgtccaa
    27661 acaggcaacg gtagagaaac cggttgaaaa acctgaagca aaagttgccg cgcccgaagc
    27721 aaaagcggaa aaaccggccg ttcgacccga acctgtaccc gctgcaaata ctgccgcatc
    27781 ggaaaccgct gccgaatccg ccccccaaga agccgccgct tctgccatcg acacgccgac
    27841 cgacgaaacc ggtaacgccg tttccgaacc tgtcgaacag gtttctgccg aagaagaaac
    27901 cgaaagcgga ctgttcggcg gttcgtacac cttgctgctt gccggcggag gcgcggcatt
    27961 gatcgccctg ctgctgcttt tgcgccttgc ccaatccaaa cgcgcgcgcc gtaccgaaga
    28021 atccgtccct gaggaagagc ctgaccttga cgacgcggca gacgacggca tagaaatcac
    28081 ctttgccgaa gtcgaaactc cggcaacgcc cgaacccgct ccgaaaaacg atgtaaacga
    28141 cacacttgcc ttagatgggg aatctgaaga agagttatcg gcaaaacaaa cgttcgatgt
    28201 cgaaaccgat acgccttcca accgcatcga cttggatttc gacagcctgg cagccgcgca
    28261 aaacggcatt ttatccggcg cacttacgca ggatgaagaa acccaaaaac gcgcggatgc
    28321 cgattggaac gccatcgaat ccacagacag cgtgtacgag cccgagacct tcaacccgta
    28381 caaccctgtc gaaatcgtca tcgacacgcc cgaaccggaa tctgtcgccc aaactgccga
    28441 aaacaaaccg gaaaccgtcg ataccgattt ctccgacaac ctgccctcaa acaaccatat
    28501 cggcacagaa gaaacagctt ccgcaaaacc tgccttaccc tccggactgg caggcttcct
    28561 gaaggcttcc tcgcccgaaa ccatcttgga aaaaacagtt gccgaagtcc aaacaccgga
    28621 agagttgcac gatttcctga aagtgtacga aaccgatgcc gtcgcggaaa ctgcgcctga
    28681 aacgcccgat ttcaacgccg ccgcagacga tttgtccgca ttgcttcaac ctgccgaagc
    28741 accgtccgtt gaggaaaata taacggaaac cgttgccgaa acacccga
//
LOCUS       DAWHRA010000006        27901 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWHRA010000006 DAWHRA010000000
VERSION     DAWHRA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27901
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.006"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(261..2183)
                     /locus_tag="WIB30_000215"
     CDS             complement(261..2183)
                     /locus_tag="WIB30_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3796357.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQTLQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPEHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLTKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(2260..2655)
                     /locus_tag="WIB30_000216"
     CDS             complement(2260..2655)
                     /locus_tag="WIB30_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ3796358.1"
                     /translation="MMRRRGKKMLFEENPIDGQFAEYECGAGGIRLARQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            2828..4135
                     /locus_tag="WIB30_000217"
     CDS             2828..4135
                     /locus_tag="WIB30_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ3796359.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            4128..4562
                     /locus_tag="WIB30_000218"
     CDS             4128..4562
                     /locus_tag="WIB30_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796360.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(4621..4890)
                     /locus_tag="WIB30_000219"
     CDS             complement(4621..4890)
                     /locus_tag="WIB30_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ3796361.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(5072..7522)
                     /gene="lon"
                     /locus_tag="WIB30_000220"
     CDS             complement(5072..7522)
                     /gene="lon"
                     /locus_tag="WIB30_000220"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ3796362.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHVHVP
                     EGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            7607..7798
                     /locus_tag="WIB30_000221"
     CDS             7607..7798
                     /locus_tag="WIB30_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ3796363.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVL
                     PPYFQNMTQVFHVFFYFSR"
     gene            7773..8876
                     /locus_tag="WIB30_000222"
     CDS             7773..8876
                     /locus_tag="WIB30_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3796364.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRSALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADMM
                     KTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
     gene            complement(8904..10649)
                     /gene="recD"
                     /locus_tag="WIB30_000223"
     CDS             complement(8904..10649)
                     /gene="recD"
                     /locus_tag="WIB30_000223"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ3796365.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVEIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(10717..11412)
                     /gene="lolD"
                     /locus_tag="WIB30_000224"
     CDS             complement(10717..11412)
                     /gene="lolD"
                     /locus_tag="WIB30_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ3796366.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIHAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(11405..12652)
                     /locus_tag="WIB30_000225"
     CDS             complement(11405..12652)
                     /locus_tag="WIB30_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ3796367.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            12857..13135
                     /locus_tag="WIB30_000226"
     CDS             12857..13135
                     /locus_tag="WIB30_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796368.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(13191..13808)
                     /gene="recR"
                     /locus_tag="WIB30_000227"
     CDS             complement(13191..13808)
                     /gene="recR"
                     /locus_tag="WIB30_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ3796369.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(13875..15413)
                     /locus_tag="WIB30_000228"
     CDS             complement(13875..15413)
                     /locus_tag="WIB30_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ3796370.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEQSLNAAVQNEQADGGSPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(15493..15846)
                     /locus_tag="WIB30_000229"
     CDS             complement(15493..15846)
                     /locus_tag="WIB30_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ3796371.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEISL
                     WLEQVPLEILLNKRGTTWRKLNALMQEEALSSKEGAVRIMSEMPSLIKRPVLEYGGKV
                     HVGFSDESYREIFKR"
     gene            15996..17624
                     /locus_tag="WIB30_000230"
     CDS             15996..17624
                     /locus_tag="WIB30_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ3796372.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            17696..18949
                     /locus_tag="WIB30_000231"
     CDS             17696..18949
                     /locus_tag="WIB30_000231"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ3796373.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYAGFSFHTDKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVEKLRSKTIIDVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            19034..19711
                     /locus_tag="WIB30_000232"
     CDS             19034..19711
                     /locus_tag="WIB30_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HEZ3796374.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
     gene            19924..20232
                     /locus_tag="WIB30_000233"
     CDS             19924..20232
                     /locus_tag="WIB30_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796375.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            20314..21345
                     /gene="ruvB"
                     /locus_tag="WIB30_000234"
     CDS             20314..21345
                     /gene="ruvB"
                     /locus_tag="WIB30_000234"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ3796376.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(21368..22096)
                     /gene="rpe"
                     /locus_tag="WIB30_000235"
     CDS             complement(21368..22096)
                     /gene="rpe"
                     /locus_tag="WIB30_000235"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ3796377.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            22242..22676
                     /locus_tag="WIB30_000236"
     CDS             22242..22676
                     /locus_tag="WIB30_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ3796378.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(22689..23552)
                     /locus_tag="WIB30_000237"
     CDS             complement(22689..23552)
                     /locus_tag="WIB30_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ3796379.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FWEHLPDPHTFPLARLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLSAVQSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(23560..24177)
                     /locus_tag="WIB30_000238"
     CDS             complement(23560..24177)
                     /locus_tag="WIB30_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ3796380.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(24340..24954)
                     /locus_tag="WIB30_000239"
     CDS             complement(24340..24954)
                     /locus_tag="WIB30_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ3796381.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQTDLQEG"
     gene            25160..26932
                     /locus_tag="WIB30_000240"
     CDS             25160..26932
                     /locus_tag="WIB30_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ3796382.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGTKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAV
                     LKIYSQPQQGTTVSLAGVSEESLK"
     gene            26929..27585
                     /locus_tag="WIB30_000241"
     CDS             26929..27585
                     /locus_tag="WIB30_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ3796383.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKVAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         6512 a         7750 c         7053 g         6586 t
ORIGIN      
        1 ttttcaggcg gtatccgcct ccctgatggc tgctgatttt aggtaaatcc gcatcggcgc
       61 acgatcctgc tggggctgat gtatatacgc ttttgccatt tgagttgcaa tggtatacgg
      121 aggcttgcgc cgccgcgccg gaaagggaca ggagacccaa ggcggcaaaa agtctgatgt
      181 ttataccggt aaaaggcggt gataacgccc gaattatacc gttattggca ggcaaagata
      241 agcaccctgt ccgcgcttct ttatcgctcg gcaaactgtt tctcgatttc agtttcaatc
      301 cgttccaatt cttcttgaca agccagccaa acctcttcga tttgggcaag ttgtgttttg
      361 acttttgtca gcagggataa ggtattttgc aatttttctt tgtttgcttc aaggtaagcg
      421 tcttcttgtg ctaaaaatgc ttcacatgcc gtctgaattt cggaaagctg cgccatttct
      481 ttttcggcac ggtctatttt ctgctgtatc ggcttgccgc gtcgggcttt ttcctgacgg
      541 atttgcgctt cgatgcgctt ggtgtctttg cggctttggc tttgtgcgga tgctgcgggt
      601 gcggcggcgg cgttttcctg tgccaaacgc cattggcggt aatcgttcaa atcgccgtcg
      661 aagttcttca gacggccttt gtcgatcagg aggaagctgt cggtagtcgc ttcaagcagg
      721 ctgcggtcgt gcgatacggc gattaatgcg ccttggaaac tttgcaacgc gagcgttaag
      781 gcgtggcgca tatccaagtc caaatggttg gtcggttcgt caagcagcaa caggttcggc
      841 ttttgccaga taatcatggc aagggcaagg cgggcttttt ctccgccgga aaaaggttcg
      901 gttttctgta acgccatatc gccaacaaaa ttgaagccgc cgaggaaatt tcggatttct
      961 tgttcgcgta cttcgggaga aagctgctga atatgccaaa cagggttttg gtcggcgcgg
     1021 atggtatcga gttggtgttg ggcaaaatag ccgatattga gtttttcgga acggacgatg
     1081 ctgccggaga gcaaatcgat tttgcccgcc aaggctttga taaatgtaga tttaccgctg
     1141 ccgttgacac ccaataaacc atagcgcgcg ccgctttcca gcgacagggt aatgtcgtgc
     1201 aaaacagttt tgccttcgta acccaaatct gcgtgttcca gcttcagcaa aggattgggc
     1261 agatgctcgg gattgtaaaa ctcaaaggaa aactcgctgt ccaaatgcgc gggagcgatg
     1321 cgttcgagct ttgccaaagc cttcatgcgg ctttgcgcct gaacggcttt ggtggctttg
     1381 gctttgaagc ggtcgataaa ggattgcaga tgtttgattt gcgcctgctg tttgacatag
     1441 gcggcttgtt gctgcgcgag acgctgcgcg cgttcgtttt ggtaaaaatc gtaattgccg
     1501 ccgtattgcg tgagcttttg ctgggacaat tcgatggttt gggtggttgc cgcattgagg
     1561 aaatcgcggt cgtgggaaat gatgatttgc gtgcagggta aagaggcaag gtggttttcc
     1621 agccacaaga cggtttctaa atccaagtgg ttggtcggtt cgtcaagcaa gagcaaatcg
     1681 gcgcggcaaa tcagggcttg cgcaagattc aggcgcatac gccagccgcc ggaaaaggat
     1741 ttgacgggac ggctgtgttc ttcttgcgaa aaacccagcc cgttcaacaa tttcgctgca
     1801 cgggccggcg cggtataagc gtcgatttct tccaatttgg cgtgatattc cgcctgtttc
     1861 ataccgtcgt tttgcgcttc cgcctgcctc aatgccgtct gaagggtctg taactcggca
     1921 tcgccctgca aaacgtaatc taaagcggaa atatccaaat cgggcgtttc ttgggaaacg
     1981 gaagcgagcc gccagttttt cggaatcgag acatcgccgc cgtcctgagt gatttcaccc
     2041 ttgattaagg caaacaggct cgatttgccc gtcccgtttt tgccgatcaa accgacgcgc
     2101 tgaccgggat tgacggtagc gttggctttg tcgagcagga ctttcaaacc gcgttgcagg
     2161 gtgaggtttt tgatttcaat cataacggaa acatcgtcgg gcgggaaaag cccgtatttt
     2221 acctgaaaat cagtgccgat gccgtctgaa atgggaaatt tacggctgaa gccaagccca
     2281 agccctgcgc ccttccgagt gcaggaaaac caatgtcctg aatgccgaat cggtattcat
     2341 gcattccacg ctgattccga ttcgggaaaa atccgccatg attttgggat ggataaactc
     2401 ctgagccgcg cccgtcccga taatcaatat ttccggatag tcaacaggtt tgatgtcgga
     2461 caacaggttt tccggagtca gatcggacaa ggttcggcat tgcgacaggc agaccgaatc
     2521 cttatgtaca agcacgggtt tatggaaact ttgcctcgcc agccggattc cgcccgcacc
     2581 gcattcatat tccgcaaact gtccgtctat cggattttct tcaaacaaca tttttttacc
     2641 ccgtcgccgc atcatctaca ccgaaagggg tgcaaaatca gacaaattca tgtaggattg
     2701 gcagatttca tctgacccgc ctgccgattt cagacggcat ttgattcaaa gtgcggcaca
     2761 attatatcgg cagcggatat tttcgtcttt caatatttac atttcagtcg gcttacaagg
     2821 agacacaatg aagccagtaa acatcggtct tttaggtttg ggtacggtcg gcggcggtac
     2881 ggctgccgtg ttgcgggaca acgcggagga aatttcccgc cgcttggggc gcgaaatccg
     2941 tatttctgcc gtgtgcgatt tgagtgaaga aaaagcccga caaacctgcc cgtccgcagc
     3001 ctttgtcaaa gatccgttcg aactggtcgc acgtgaagac gtcgatgtcg tcgtcgaatt
     3061 gttcggcggt accggcattg ccaaagatgc ggtgttgaaa gccattgaaa acggcaaaca
     3121 catcgttacc gccaacaaaa aactgctcgc cgaatacggc aacgaaatct tcccgctggc
     3181 ggaaaaacaa aacgtcatcg tccaatttga agcggcagta gcgggcggta tcccaatcat
     3241 caaagccctg cgcgaaggtt tggcggcaaa caggattaaa tccatcgccg gcattattaa
     3301 cggcaccagc aacttcatcc tctccgaaat gcgcgaaaaa ggcagcgcgt ttgccgatgt
     3361 actgaaagaa gcgcaggcat tgggttatgc cgaagccgat ccgaccttcg acatcgaagg
     3421 caacgatgcg ggccataaaa tcaccatcat gagcgcactg gcattcggca cgccgatgaa
     3481 cttttccgcc tgctacctcg aaggcatcag caaactcgac agccgcgaca tcaaatacgc
     3541 cgaagaactt ggctatcgca tcaaactgtt gggcattacc cgcaaaaccg gcaaaggcat
     3601 cgagctgcgc gtccacccta ccctgattcc cgaaagccgc ctcttggcaa acgtcaacgg
     3661 cgtgatgaac gccgtgcgcg tcaacgccga tatggttggc gaaaccttat attacggcgc
     3721 gggcgcgggc gcattgccga ccgcttccgc cgtggttgcc gatatcatcg acatcgcccg
     3781 cctggttgaa gccgataccg cccaccgcgt accgcatctg gcgttccaac ccgcgcaagt
     3841 ccaagcgcaa accatcctgc ctatggacga aattaccagc agctactacc tgcgcgtcca
     3901 agccaaagac gaaccgggca cgctggggca aatcgccgcg ctgttggcac aagaaaacgt
     3961 gtccatcgaa gcactgattc aaaaaggcgt gattgatcag accactgccg aaatcgtgat
     4021 tctgacccac agcacggtcg aaaaacacat caagtcggca atcgcagcca tcgaagcact
     4081 ggattgcgtg gaaaaaccga ttaccatgat ccgcatggaa agcctgcatg actgagccga
     4141 aacacgaaat gccgacggaa gagcaggttg ccgcgcgcaa aaaagcaaaa gccaaaatcc
     4201 gcaccatccg catttgggcg tgggtcattt tggcgttgct cgctttaacc gccctgctct
     4261 cccaatgcgc gatgtccaaa ccgcaggcaa aacagaaaat tgtcgagtct tgcgtgaaga
     4321 atattccgtt tgccgaaaaa tggcaaaacg atttgcgggc ccgcggttta gattcaaaca
     4381 atacccgcct cgccgtcgac tactgcaaat gtatgtggga gcagcctttg gacagattga
     4441 gcgagaaaca gattagatcc ttcggcaaac tcggcgcaca agaacagctt gacctgctcg
     4501 gcggcgcaaa tgcctttgaa gcacgtgaca agcagtgtgt tgccgatttg aaatcagaat
     4561 aatgtggacc gataaaaaag ccgattcttt aaagaatcgg cttttttcat aaaaaacggc
     4621 ttacagtgca tctttcaatg ctttacctgc acggaattta ggcgttttgg cggcggcaat
     4681 ggtcagaggc tcgccggttt tggggttgcg gccttggcgt tccgcacgtt cgcccacgta
     4741 gaaagtaccg aaaccgacca aagtaacggt gtcgccttgt ttcagggcgg tggttactgc
     4801 attggtagtg gcatccaaag ctttttgtgc ggcggctttg gaaatgtcgg cttcttgagc
     4861 aatcgcttcg atcaattcag acttgttcac aatcagtccc ttcctgtctt aaaaaatgat
     4921 gaaatgcccg aatactcggg gtttgtactg cttgagcaac tttcgcttta tagcaattct
     4981 gaaattgccg tgtcaagcaa aaaatacgga atcaccctat ttgacaggct ttcaggacga
     5041 aaccgcattt ttacaacaca tttcctgcgt ttcaatgttt ggttgccctg ctgcggggtt
     5101 ttggttttga agcggattcc gccgccgctt ccgcaccaga aggttctgcc caaggctcag
     5161 gctggctttc caaacccaga gccaatacct cgtctatcca tttgaccgga tggatggtca
     5221 ggccggtttt cacgttttca gggatttctt ccaagtcttt gaggttgtct tttggaatta
     5281 aaacgtgttt gatgccgccg cgaagcgcag ccaacagttt ttccttcaga ccgccaatcg
     5341 gcaggacttc gccgcgcagg gtaatttcgc ccgtcatggc aacatcggcg cgcaccggga
     5401 ttttggtgaa tgcagacacc atcgccaaag tcatggcaat gcccgcactc ggaccgtctt
     5461 tcggcgtcgc accttcggga acatggacat ggatgtcttt tttctcgtaa aaatcaggag
     5521 ccaagcccac tgattccgcg cgggagcgga caaccgacca cgctgcggac acggattcct
     5581 tcatcacatc gcccaactgg ccggtgcact gaatcacgcc cttacccggc aatgctgcgg
     5641 cttcgacggt cagcaattcg ccgccgactt ccgtccacgc caaaccggtt acttgtccga
     5701 tacggttttc gctttcggca acgccgtaat cgaagcggcg cacacccaaa tagtcgtgca
     5761 gatttttctc atttacttta accgccctgg gcttggcttt tccggttttt ttggtttttg
     5821 agtcttcgtc caaggtaatc tgcatcacca ccttgcggca gattttggca atctcgcggt
     5881 cgagcgaacg cacgcccgcc tcgcgggtgt agtaacggat aatatcgcgc accgcgcttt
     5941 cttcgattgc caattcccct tcttttacgc cgttgcgctt catttgcttc ggcacgaggt
     6001 actgcatcgc gatattgatt ttttcgtctt cggtatagcc ggacagacgg atgatttcca
     6061 tacggtcgag cagcggtgtc gggatattca ggctgtttga agtcgcaata aacatcacat
     6121 cgctcaaatc gtaatccact tcggcataat gatcggcgaa cttgttgttt tgctcgggat
     6181 caagcacttc gagcaacgcg ctggcgggat cgccccggaa gtcgctaccc aatttgtcga
     6241 tttcgtcgag caggaacaat gggtttttta cgccggcttt tgccatattc tgcaaaatct
     6301 taccgggcat ggagccgata taggtgcggc ggtgtccccg gatttcgctt tcgtcgcgca
     6361 cgccgcccaa agccatgcgg acatatttcc gccccgttgc tttggcgatg gattcgccca
     6421 aagaggtttt gcccacgcct ggagggccga ccaggcacag aatcgggcct ttgagtttgt
     6481 ccatacgttt ttggacggcg aggtattcca aaatccgttc tttgactttt tccaggccgt
     6541 agtggtcggt atccagcacc aatccggctt tggcgatgtc tttgctgacg cgggattttt
     6601 tcttccacgg cagttcgagc agggtgtcga tgtagttgcg tacgacggtg gattcggcag
     6661 acatcggcgg catcattttg agttttttca gttcggacag gcatttttct tccgcttctt
     6721 tgctcatccc cgcctttttg atgcctgctt ccaaggcatc cagttcgccg ttttcgtctt
     6781 cttcgcccaa ttctttgtgt atcgctttaa tctgttcgtt cagataatat tcgcgctggg
     6841 atttttccat ttggcgtttg acgcgtccgc gtatgcgttt ttcggcctgc ataatgtcga
     6901 gttcggattc cagctgtgcc agcaggaatt ccatccgttt gccgatttcg ggaatttcca
     6961 aaatctgttg gcgttgcgcc agtttcaact gcaaatgcgc tgcgaccgta tcggttagcc
     7021 ggctgttttc ggcaatgccg ttaatgctgc cgataatttc ggcggggatt tttttattga
     7081 gtttggcgta ttgttcaaac tgcgccaaca gggtgcggcg cacggcttcg aggtcggtat
     7141 tgccgcccgt gtcttcttcc acgaccgtct ctatatggga aacgaacaga ccgcccgtgt
     7201 cttcaatggt cagaacacgt ccgcgataca gcccttcgac caatactttt accgtgccgt
     7261 cgggtagttt caacacttgc aggacttgtg cgaccgtacc ggtctgatac aggtcggcgg
     7321 caaccggttc ttctagcgcc gcatcggttt gcgccaacag gaaaaccggc tcctcgcggg
     7381 taatggcgtt ttccagtgcg gcgatggatt tcggcctgcc gacaaacagc ggcagaacca
     7441 tatgcgggta aacgacgaca tcccgcaaag gaagggttgc caaggcggca tattcctcaa
     7501 aatgcttttc tttttgtgtc ataggtactc tcttgtgtct gacagattgc cgattttcgc
     7561 gtacattggg gttgaaggta ttatttcaag catatgtggt ttatttatgg agtttgatgc
     7621 gatgccgtct gaaacattcc ggcttcagac ggcatgggct tggaaagaca aggcgggaac
     7681 aaaaaactgt tctgtgttgc cgctccttgc tgtaccatcc gtatggtttg cggttctgcc
     7741 gccctatttt caaaacatga cgcaggtatt ccatgtcttc ttttattttt cccgataacg
     7801 ggttcgaatg gcgcaacgaa agtctgcaaa agccgcccaa aggttggcat tatgtccgcg
     7861 aaagtggtgc aaacggcatt ttgaaggctg cctatcaaaa tattgcaact gtctggcagg
     7921 gcgatttcca caatgccaaa caggtgcttt ccgcaatgaa gaagaaggtc cgcaaacctg
     7981 ccgccgtccg ttccgatgcg gatatcgccg cccttttcca cgcccaccgt atgaagcagg
     8041 cgcagcagag ccgtattctg aatatgcttg ccgttgaaat ccgccccggt tttgtgttgg
     8101 acaacaaacg cgcgcccgat atacgctccg ctttgctcga cgtgtacgga gaggcggacg
     8161 gcaaaccgtt tttcctgccg ctcaatctgc tgctggggtt tatgggtgcg cacgagtggc
     8221 ataagaaagg ggttgccgtt ccgcagctgg gcggcagcat acacgttcct ttcggcgtat
     8281 tctcgccgtt gcgcggcgaa tacctcgacc tgctcgccca tgcgccgtca acgggttttc
     8341 agacggcatt cgatatcggg acgggctcgg gcgtgcttgc cgccattttg gcgaaacagg
     8401 gcattccttc cgtcatcggc acggatacca atccgaaagc cgtcgcctgc gcccgtgcca
     8461 atattgcccg tttgggcttt gaaaaacagg ttgagatacg ggaaaccgat ctgtttcccg
     8521 aagggtttgc cgatctgatt gtctgcaatc caccctggct tcccgccaag ccgacttccg
     8581 ccgtcgaatc cgcgttatac gaccccgaat ctgcgatgct ggctgcgttt ttgcgggatg
     8641 cgccgaaaca tctgaatccc gacggagaaa tccgcctgat catttccgat cttgccgaac
     8701 atctgcacct gcgtccgtcc gattttctgg ataaggcatt tgctcaggcg ggtttgcgtg
     8761 ttgccgatat gatgaaaacc aagccgaagc acaaaaaagc cgcgaatccg agcgatccgc
     8821 ttgcttttgc gcgaacccgg gaaaccactt tcctataccg tttgaaaaag gcataagggg
     8881 cggcggcgcg gcattcgggc ggattattct tgtgaaaata cccgctcgag catactgccc
     8941 aatgccgtct gacgcgtttt gacggtggcg gcagcttgcc ggaaggcttc ttccccgccg
     9001 aagaatacga acttctctcg cgcgcgggta atggcggtat ataacagctc cttactcaat
     9061 ccggacaatg catcgtcccc ttcgtccgaa ggtgcggcgg aaggcggcag cagccatact
     9121 tcccggtatt ccgaaccttg gcttttgtgg acggtcatgg cgaatgcggg ttcaaattcg
     9181 ggcaggcagc ttaccgctac ctttttaaat ccgtccgcat cggcaaaata ggcggcaagg
     9241 ctgccctgcc gtccgacatc ttccataatc agtccgatgt cgccgttgaa cagttcaagc
     9301 gcgtagtcgt tctgcctgat cataatcggc tctccggcaa aatatgccaa atgttccggt
     9361 atgttcattt tgcggcgtac atggcggcaa taggcttcgt tgaagtcttc cgcatcctgc
     9421 cgccaagctg ccagaaccac gatatccgaa atgcccgcgt atgcggcttc gatattgccg
     9481 tcttttaccg cctgccaata ggctttgtgt gcccggtaca acctttcgac tcgagcgttc
     9541 ggactgcatt ccgaatgttc cagttcgtcc ggaaaccggt caaacaatgc ccacgcccct
     9601 tcatcgcccg atacggcggc acgggcaagg cagccgatgc cgctgttgtc gccgaagcgg
     9661 tggctgaacg acagatgggc ggtgttttgc gccaacacgg gtggatttgc gctgacgctg
     9721 aaaccgtgtt ccggaaggaa gccggccagc ctttggtgcg tttctccgtc caaaacggtt
     9781 ttttgtgaca aaacggacag taccgcccct atttcgacgg acgggagctg gttttcatcc
     9841 cccagcagaa tcacgcgcgc gccggttttg accgctttta aaagttgcag catcaatgcc
     9901 gtatccagca tagaggcttc atcgataacc aatacgtcaa acggcagcgg gtaaacaggg
     9961 ttgaacgccg cctgcatttt gggcgggcgc agcttcagca gtcggtggac ggtttgccct
    10021 tccagtttga gcaaatggcg gcggacggcc tccggcgcgt caaaaccgtt gattgcacgg
    10081 tgcagcgcgc gcgccatatg tgccgccgct ttgcccgtcg gtgcggcaag cgcgatgttg
    10141 ggaagatttt cgtcttcacc gcaaatcagc gccagcagtt tggcaaccgt tgtcgttttg
    10201 cccgttcccg gcccgccggt aatcaccata aaagactgca acagtgccaa ggcggcggca
    10261 tcgcgctgcc cttcgctgcc cgtgccttga aaccattttg cgaggttttg cctcgcgcct
    10321 gccgcgtcgg gggcggatgt gccggctgcc gccaagcgtt ttatctcggc agccaaatcg
    10381 tattccaact gccacatcct gcccaaaaac agccttctgc cttccaaaat caaaggcgcg
    10441 gcggatgttc cgacaacggg tgcgagtgcc gacagcgcgt cagcctcgcc accgctcaaa
    10501 cggataaacg aatgaccgtt ttgcaatgcc tgaaacaggc gttcggtgca gtttgcaagc
    10561 acttcgtcgc cggaacccgc atagtgttcc aaaaaacgga ttgccgccct tgccgccgct
    10621 tgggcaaatt catctgtctg ccgttccatt ttattcctta tccaaatgcc gtctgaaggc
    10681 gtggggcttc agacggcgcg gtgttgtttc ggctgtttag gcgtttgcgc cctgtttggg
    10741 gtgcaggctg ccgtctttca tgaccatcac gcgctcgaag cggccggcga gttcgtcgtc
    10801 gtgcgttacg accaccagcc ccgttcccaa ttctgttttc agttccagca tcatatccaa
    10861 aacattcctg gcgttcgcac gatcgagatt gccggtcggt tcgtcggcaa gcaggcattt
    10921 gggttgggta accaaggcgc gtgcaatggc ggcacgctgc cgctcaccgc ctgaaagttc
    10981 gcccgcgcgg tgcgtcgaac ggtgtttcag tccgaccttt tccagcatcg ccattgccgc
    11041 ctccgcagcc tcttcacggc tttttttgcc gatcagaagc ggcatcatca cattttccag
    11101 tgccgaaaat tcaggcagaa gatggtggaa ctggtacacg aaaccgagat ggcggttacg
    11161 taaatcgccc aaacgccgct ggtttaaggt acgcaaatcc tcgcccatca gcagcaccct
    11221 gccttcagac ggcatatcca gcccgcccaa aatatgcagc agcgtcgatt tgccgctgcc
    11281 cgaagaaccg atgatgccgg tgctttcccc tgcgtggatt tccaaatcca agccgtgcag
    11341 cacccgaacg tccaaaccgc cgtcccgata gcgtttgccc acgccttcgc atttcaaaat
    11401 caactcactc ataacgcaaa gcctccgccg gttgggtttt tgacgcgcgc cggctcgggt
    11461 agagcgtggc aacgaaagac agtcccaaag aaatgcaggc aatcagggca acgtcgccca
    11521 tatcgacatc gctgggcagg tagtcgataa aataaacctg cgaattgatg aggtggacac
    11581 cgagcaggtt ttcaaaaaac gccacgaccc tgccgacgtt ccaacccaaa agcacgccgc
    11641 agaccacacc cgccagcgtg ccgaaaaagc ctgaaaacgc gccctgcacc ataaaaatct
    11701 tcatcacgcc gccaggggaa agacccaaag tccgtaaaat cgcaatgtcc gcctgctttt
    11761 ccgtaaccgc catcaccaag gaagagacaa ggttgaacgc cgccacggcg ataatcagcg
    11821 tcaggatgat gaacatcatc cgtttttcca actcgaccgc ctcaaaatag cttcgattgc
    11881 tgaacgtcca atcgcgcacc caaaccgtat ccctttgcgc ctccggaatc agtgttgccg
    11941 tcaaggcggg ggcgttttgc gggtcggcga gtttcagccg cagccccgca acttccttat
    12001 ccaagcggta cagtacgcgc gcgtcttgga tatgcgtcat cgccaaggag ttgtccactt
    12061 cgtaaacacc cgtcttaacc agaccgacca cggtaaactg tttcaacctc ggtacgactc
    12121 cggcgggcgt aacattgccc tccggcgtga tgacggtaac tttattgccg acttccgccc
    12181 ccaaagcctc cgccaagccg acaccgagga taatgtcaaa ctcgcccgga atcagatctt
    12241 caaatttgcc tgccggcatt ttgtcgccgt attccaccac tttgcgttct tcagacggca
    12301 aaatgccgcg catctgcacg cccctgattt cgcccgcatt ggccagcaat gcctgattgg
    12361 aaacataggg cgcggcagcc aaaatacctt tgcggttttc ggtaaaccga agcaggttgc
    12421 gccaatccgt atccgtatta tcgatatagc cgatttcggc gtgcggcgcg acattcagga
    12481 gctgcccgcg tatttctttc tgaaagccgt tcataaccga caagacgaca atcagcgcgg
    12541 ttacgcccaa ggcgattccg gcaatcgaaa ccatcgtgat aaacgacata aagccgttgc
    12601 gctttttcgc cctgagatac ctcaagccta tccaagcctc tagagaaaac ataacgctac
    12661 cttaaaaatg tctgcaaacg tgccgccccg gacggcggtt tgggggcggc ggcaaaagtt
    12721 tattgtaccg taaaacccag gcagcgtccg aacgcccgag tgcgggggac ggggcggcag
    12781 agcgggcgga aaacttgcac aacgcgtcaa actgccctat cctttccttc agaaaaaccg
    12841 tttcttgagg aaaacaatga atatccgaac tgcttttgct ttgtgcgcca tcgccttatc
    12901 cgccgctgcg gctgcctacg ccaaagaaat caaaatcgat gccaacaaca cgccttattc
    12961 cgaagccgac gcgcaaaagc tggcggcaac ggcagtcggt atgggtgtta aggaacctat
    13021 cagcctgaac ggcggcagcg gcagcattac cgtgtccggc agcagcgcga cgcagtgcgt
    13081 gttcaaagtc ggcaacggag gcgcattgca gattcaaggg ctaaactgca agtaaaccgc
    13141 ccggaaaaat gccgtctgaa ggcttcagac ggcattttgc attggcggcg ttatgccccg
    13201 ccttctttaa tcaggcggcg ttcgtacacc gcctgcgcca gcgttcccgc atcgacatat
    13261 tccaattcgc cgcccaaggg aatgccctgc gacagcctgc tgactttgta aggcaggttt
    13321 ttaaaaaact cggacaggac atacgccgtc gcattgcctt ctgcggtaaa agcggttgca
    13381 ataatgattt cttcgacttc cccgccgccc agccgttgcg ccagcctgtc caatgcgatg
    13441 gcggatacgt ccattcccaa tgccgtattg atttgcccca tcaggacgaa atacagcccg
    13501 tcgtggcagt ttgccgcttc catattcgac acgtcggcag gcatatgcac caccatcagc
    13561 cgccgcccgt cgcgtgtttc atcggcacaa atatcgcaca atccgccttc gcaaaacgtg
    13621 ttgcacatcg cgcaatggta aacctgcttc aatgccgtct gcaaggcatc caccagtttt
    13681 tcagcctctt tgcgcttgtg ttggagcaaa tgatacgcta tccgctgtgc cgatttcggc
    13741 ccgacgttgg gtaaaacctt cagcgcgtcg atcaatcctt ggaaggcatc ttgttttttg
    13801 tggctcatca tattccgccg tatgggaaaa cggccggaat attccgaccg ttattttgtc
    13861 aacaaaagtg tcaattactg accgtcgccg ttgtcgaccg attgcgctcc tttggtctgt
    13921 ttgattttgc cgttgaaata acgtatcaac aagtcgaaag tattggcaga ctgctgttgc
    13981 gccaaagcct gttttgcaag cggaagctgt gcggcgatat catccggcgg ggttacagcc
    14041 tgtacttcga caatcacggg tgccggcaga ccgatcagcc tgacgtaggc gggtttgccg
    14101 tttgccggtt ttgctttcag cagttccgca taagcctcgg gcggcatgga ctgccttgcc
    14161 tgctgtgcgc ccaaaacgga cacttccgac catttcacgt caacagcctt gccgccgttc
    14221 agttgggtaa gcacgtcttt tgccttgttt tcggcaagtt tggcggcttc ggtacggata
    14281 taagcctgac gtaccgcgtc tttggcttcg gcaaacggca gggttttctc ttcgcggact
    14341 tctttggcgc ggacgaccca cgcggtttcg ctgttgatgg tcagcacttc ggaattgtgt
    14401 tttttcttca atacgtcgtc gctgaatacg gcattgatca ggttttcggg cataccggac
    14461 atttgcgcgt cctgcctact cagccaagtt tcttgggttt cgactttcaa accgctgttt
    14521 ttggcggctt cggcaagcga ggaaggatgg ttgaacgcat cgtcgcccaa tttttctttt
    14581 gccttgttga agtcggcaac cgcctttttc attttcaatt cgttttcgac ggcggctttt
    14641 tcctgctcga aagaaggttt ggcttcattt gccggcaaac gcgccacgcg ctcttcaaac
    14701 gcatttttca cttctgtttc gctgacggtc tgtttgtctg caaagtcttt cagattcaat
    14761 gcgacatatt ccaatttgac cgctttggga agcaggtagt cttttttgtt tgcgttataa
    14821 aacttctgca aatcggcttc agacactttg acttgggcga tgaattcgtc ggggttgaaa
    14881 gtgtgcgaac ggatggtgcg gttgacctgc gtcagcctga tcagctgttc cgcctgcgcg
    14941 tcgccgacca atacgccgtt ttggacgagg tttaccaaat tctgcaaggc aaactgatcg
    15001 cggatttctt cgacaaactg gtcttcagac atatggcgtt gcgacaggta gcggtttaaa
    15061 agcgcgtggt cgaatttgcc gtttgcgtcg tggaaattgg gatcgtccac gataatctgc
    15121 ttgatttgtt cggaagaaac cgaaatgccc atcagcttcg cgccctgttt caggtaggcg
    15181 cgttgtagca gggattggaa caccgcgtcg cgcgaagggc tgccgccgtc cgcctgctcg
    15241 ttttggacgg cggcgttaag cgactgctcg ctgatttttt cgtcgcccac ttggacgatg
    15301 tagtcggcgc ccggatggga taccgtgctg accccgaagc cgacgaaggt caatgcaatc
    15361 aggcctaata agacttgggc gggcgttctg tatttttcga tggaatggaa catattttaa
    15421 atcgggatat agaatgggaa attcaagtcg ggtattgtaa cggtttttat acgcgcctgc
    15481 acgaaggctt aactaacgtt tgaatatctc tcgataagat tcatcgctga agccgacatg
    15541 aacctttccg ccgtattcca gtacgggacg tttgataagg ctgggcattt cggacattat
    15601 tctgaccgca ccttcctttg aggacagggc ttcctcctgc atcagtgcat taagtttgcg
    15661 ccatgtagtc ccccgtttgt tgagcagtat ctccaaaggc acctgctcca gccacaggct
    15721 gatttccgct tcagacggcc tctgtttttt gaaatcccga aattcaaact ccaagccgta
    15781 tccggcaagc ctgtttttgg cttttttgac cgtatcgcaa tttgggatgc cgtgaaggac
    15841 tatcatttgg aaaccttttg tctgaaataa taaaacggat attttactat aagtgtctga
    15901 aaatttgccc gtctgtttca gacggcgggg cggttatgtt acaatccgaa aattcgaaaa
    15961 atttaatctc ttgttcaata aaggctttac caatcatgat ttctaccaac ggcatcacca
    16021 tgcagttcgg cgcgaagccg ctgtttgaaa acgtatccgt aaaattcgga gaaggcaacc
    16081 gttacggcct gatcggcgcg aacggttcag gcaaatccac cttcatgaaa atcctcggcg
    16141 gcgatttgga acagacggcc ggcgaagtgg cgattgaaaa cggcgtgcgt ttgggtaaat
    16201 tgcgccaaga ccagtttgcc tacgaagaca tgcgcgtgct ggacgtggtg atgatggggc
    16261 ataccgaaat gtgggcggcg atgaccgaac gtgatgcgat ttacgccaat cccgaagcca
    16321 ccgaagacga ctacatgaaa gccgccgaac tggaagccaa gttcgccgaa tacgacggct
    16381 acaccgccga agcgcgtgcc gccgaactgt tgagcggcgt gggcatttcc gaagatttgc
    16441 acaatgcgaa aatggcggaa gtcgccccgg gcttcaaact gcgcgtattg ctggcgcagg
    16501 cgctgttctc caagccggat gtattgctct tggacgaacc gaccaataac ttggacatta
    16561 ataccatccg ctggttggaa ggcgtgttga accaatacga ctccacgatg attatcatca
    16621 gccacgaccg ccactttttg aacgaagtct gcacgcatat ggcggatttg gactacaaca
    16681 ccatcaccat ctatccgggc aactacgacg attacatgct cgcttctgcc caatcgcgtg
    16741 agcgcgccct gaaagacaac gccaaagcca aagagaagct gcaagagctg caagagtttg
    16801 tcgcacgctt ctctgccaac aaatccaaag cccgtcaggc aaccagccgt ctgaaacagg
    16861 ccgacaaaat caaatcggag atggtcgaag tcaaaccttc cacccgtcaa aacccgtata
    16921 tccgttttga agccgatgaa aaagccaagc tgcaccgtca ggctgtggaa gttgaaaaac
    16981 tggcgaaacg ctttgaaacc cagttgttta aaaacctgaa cttcatcctt gaagcgggac
    17041 aacgcctcgc catcatcggc ccgaacggcg cgggcaaatc caccctgctg aaactcttgg
    17101 ccggcgcgta caaccccgaa tattcagacg gtctgctgcc ggacgaaggc agcatcaaat
    17161 gggcggaaaa agccagcgtc ggctactatc cgcaagatca cgaaaacgac ttcgatgtcg
    17221 atatggattt gagcgaatgg atgcgccaat gggggcagga cggcgacgac gaacaagtca
    17281 tccgcggcac tttggggcgt ttgctcttcg gcagtaacga tgtcgtgaaa aaagtgaagg
    17341 ttctctccgg tggtgaaaaa ggccgtatgc tttacggcaa actgttgctg ttgaaaccca
    17401 atgtcttggt catggacgaa ccgaccaacc acatggacat ggaaagcatc gaatccttga
    17461 acatggcgct ggaaaaatac aacggcacgc tgattttcgt ctcccacgac cgtcagttcg
    17521 tttcttccct ggctacccaa atcattgaat tggacggcaa aggcggatat gaacactact
    17581 tgggcgatta cgaaagctat ctcgagaaaa aaggcgtagc ataaccgccg gttggaacaa
    17641 tgccgtctga agcggcttca gacggcattg ttgataactt taaaatagga agcatatgca
    17701 gacttatctc gtcggcggtg ccgtccgcga ttatcttttg ggcttgcccg tcaaagaccg
    17761 cgattgggtg gtcgtcggcg cagacgcaca aaccatgctg gcgcaaggct tccagccagt
    17821 cggcaaagac tttcccgtgt ttctccatcc cgaaacacac gaagaatacg ccctcgcccg
    17881 caccgagcgc aaaaccgcca aaggttacgc cggcttcagt ttccacaccg acaaagacgt
    17941 tacgctggag caggatttga tgcgccgcga cctgaccatc aacgcgatgg cgcaagatgc
    18001 ggacggcaag attatcgacc ctttcggcgg acaacgggat ttggcggcag gcattttgcg
    18061 ccacgtttcc ccggccttcg ccgaagaccc cgtccgcatc ctgcgtactg cccgctttgc
    18121 cgcgcgttac aagtttgaaa tcgccgaaga aaccataaag ctgatgcggc agatggtgga
    18181 aaacggcgaa gcggacgcat tggttgccga acgcgtctgg caggagtttg cgaaaggttt
    18241 gatggaaaaa aatccgcgca aaatgattga agtgttgcgc gaatgcggcg cgctcaaagt
    18301 cttgctgccc gaagtcaatg ccctcttcgg cgtgccgcaa cgcgccgact accatcccga
    18361 aatcgacagc ggcatccata ccctgatgac gctgcaacgc gccgccgata tgggtttgag
    18421 cctgcccgaa cgctatgccg ccctgctgca cgacttgggc aaagccaaaa caccgtccga
    18481 catcctgccg cgccaccacg ggcacgacct cgccggtgtc gaacccgtgc gcgaagtcaa
    18541 tcagcggctg cgcgcgccga aacattgcgc cgagcttgcc gaattggttt gccgttggca
    18601 catcatcttc catcaggttg aaaaactccg cagcaaaacc attatcgacg tattgaaaaa
    18661 aaccgatgcc ttccgccgcc cagaacgttt tcagacggca ttgaacgtct gcattgccga
    18721 cacgcaaggc cgtctgaacc gcgaacacac gccctacccg caacgcgcgc actggctcgc
    18781 cttactcgaa gccgccaatc aggcggattc gggcaaaatc gccgccgaat gccgcgcgca
    18841 gggaaaagcg caccttatcg ccgaacaaat cgaccgggcg cggctggcac aaatcgcccc
    18901 attgcaaaaa gcgtttcgag cggcgcaaga caaaacagaa aaacattaaa acgtccaatg
    18961 cagccacttt tatagtggat taacaaaggg gcttaggaag caaaatcatc aaaaaacctg
    19021 ataagctcct aatatgaaac taaaaaataa gtatcaaaag ttcagcaaaa tttcagagca
    19081 aaagtttaga gaaataatcc gctgtttcgc tcttgatttg accgcttccg atactgctaa
    19141 aatgaccggc attagtgtac gcggtatcaa tcctattttc ctgaaaatca gacacaggat
    19201 tgctgctctg tgcgaacaaa gctcaccact gtccggtgtg gtcgaattag atgaatctta
    19261 ctttggtgcg cgacgtatca ggggtaagcg cggtcgcgga gcgtcaggta aaaccatcgt
    19321 atttggcata ctgaaacgca atggcgtggt ctatacggaa atcgttcccg acgcttcgaa
    19381 agcctcacta atcaaggtca tacgtggtca catatctgct gacagtgaaa tcaatactga
    19441 cggctggaaa gcatatgacg gtcttgtcga tatgggctat gagaagcatt accgtgtcca
    19501 tcatggttcc aatgagtttg ctcgaggaaa gcaacatatc aatggtattg aatctttttg
    19561 gagttatact aaggggcgtt tggctaaatt tcatggtatt tccaaagaga tgttttatct
    19621 gcatctcaaa gaaacggagt ttaggtttaa ccaccggcat gaagatttag gtaaaatatt
    19681 aatgaagatg cttcgaaata acccaattta atatttttgc ttcctaagcc cctaataaaa
    19741 atcaggacaa ggcgacgaag ccgcagacag tacagatagt acggcaaggc gaggcaacgc
    19801 tgtactggtt tttgttaatc cactataaag ttttgaggac gatacccaat ccaagctttg
    19861 caacagccgc cgccatatcc gctataattc acgcttcagc cattccgccc ccgacataaa
    19921 atcatgaccc tgaaaaccga tttattgcct aaaatcaaca acgaagatta tcaacgcctc
    19981 atcctcaaac acagtgcgga atttagcggt ggcgaaatcc gcctgttgaa cgaaatcctc
    20041 gaaaaattca atttcgacgt tgttcaggcg caggcattgg cgcaagccgt catgcagcaa
    20101 atccgcttcg accccaacgc ctaccacatc gacagcgacg acgaagacac caccggcatc
    20161 tgcccccact gcatcaaccc gcctatgccg cccctgcgcg actatctcgt ttggcgcgaa
    20221 acccgcggat aaaacgcttt tgaccgttat cttttcaatg ccgtctgaaa cgccgccgac
    20281 cgttcggacg gcatacccga caaagggaac actatgctgc aaaccgacaa cctgaccgcc
    20341 gcgcaaccgc aacgcatcgt tgccgcccaa accgcctccg cacaggaaga actgctcgaa
    20401 cgcgccctcc gccccaaaac gctggacgac tacatcgggc aagacaaagc caaagaacag
    20461 cttgccattt tcatccaagc cgccaaaaaa cgcggcgaag cactcgacca cgttttgctc
    20521 ttcggcccgc ccggactggg caaaaccaca ctggcgcaca tcatcgccaa agaattgggc
    20581 gtaaatttgc gccaaaccag cggccccgtc ctcgaacgcg caggcgacct cgccgccctt
    20641 ttgaccaacc ttgatccgca cgatgtattg ttcatcgacg aaatccaccg cctcagccct
    20701 gttgtcgaag aaatcctcta tcccgcgctc gaagactacc ggctcgacat tatgataggc
    20761 gaaggacccg ccgcccgttc cgtcaaaatc gacctgccgc ccttcacgct catcggcgcg
    20821 accacccgcg ccggtatgct gaccaatccg ttgcgcgacc gcttcggcat cgtctcccgc
    20881 cttgagtttt acgaaaaccg agaccttacc accatcgtca gccgttcggc acaactgttg
    20941 cagctcgata tgtccgaaga aggcgcggaa gaaatcgcca aacgcagccg cggtacgccg
    21001 cgcatcgcca accgcctgtt gcgacgcgtg cgcgatttcg ccgacgtgaa aaacaacggc
    21061 acaatcgacg gcggcatcgc cgatgccgct ttaagtatgc tggacgtgga cgcgcagggg
    21121 ctggacgtga tggacaggaa atttctcgaa gccgttttgc acaaattcgg cggcggcccg
    21181 gtcggtttgg acaatgttgc cgccgccatc ggcgaatcta cagacaccat cgaagacgtt
    21241 atcgaaccct accttatcca acaaggcttc ctgcaacgca ccccgcgcgg caggatggcg
    21301 accgaacgcg cctacctgca tttcgggctg cccgtcgaaa aataacgcaa tgccgtctga
    21361 aacagagcta attttcagac ggcatttcta tttcaatcat tggcgcaagg ttcagcctgc
    21421 cgcttttttc cagttccgcc ctcatcgcat caatcaccgc cttatagtct ggtttgccga
    21481 aaatcgcaga accggcaaca aaggtatccg caccagctcg ggcaacggcg gcaatattgt
    21541 cggttttgat gccgccgtcc acttcgatgg cgatgtgccg cccgctttgt gcttcgtacc
    21601 gatccagcat cgcccgcacc cggcggattt tttcaagggt gtgcgggatg aagctttgtc
    21661 cgccgaatcc ggggttgacc gacatcagca aaaccatatc cagcctgtcc aatacgtttt
    21721 ccaacagata tacgggcgtt gccggattca acaccagccc cgcctgacag cccatatcac
    21781 gaatcaggct caagctgcgg tcgatatggc ggctcgcctc gggatggaac gtgatgattg
    21841 atgctcctgc tttggcaaac gactgaatca ggtcgtcaac gggttcgacc atcagatgca
    21901 catcaatcgg cacgcttgca taaggcttca acgccgcgca aaccataggg ccgaaggtca
    21961 ggttcggcac ataatggttg tccatcacgt caaaatggat cagatctgca cctgccgcaa
    22021 tgacgctttc cacctcttct ccaaggcggg caaagtctgc cgacaaaata ctgggtgcga
    22081 tacggtaggc ggtcatattt tttccttcaa tgcctttacg ggcggactca acgcgggaaa
    22141 gccccgaaac agcattatta acaaaagtta actgccataa taccatcttt cacacggcaa
    22201 tcaagtatat tgtccgaaat atctggatta aaggaaaagc cgtgccactg cccgctccct
    22261 gccgtttcgc caaacctgcc gcctcttttt taagtatggc tttgctttcc tgccagcttt
    22321 cccacgccgc cacggcttat atccccctga acgattttca accgaactgc gacatacgcc
    22381 gactcggact gacacagggt cagcacaatg aactgcgtaa aatccgcgcc gccttcaaaa
    22441 tggcgggcga cagggcgcgt ttgaaggtta tgcattccga acacagccgc cgccggtctg
    22501 tcgtcgaaat catttcttcg gatgttttta atcggaacga ggcgcgcgat tatgtcgaaa
    22561 gccgctattt gtccggtatg gattttgcgg tggacgaatt ggaaatccaa caccggttct
    22621 tccatatcct cacaccgcaa cagcagcaaa tgtggctttc ttcctgcctc aaataatccc
    22681 cgaaacgctc acaacgcccg ttgtttcggc agcctgcccg cccagtcgca ggcaaactgc
    22741 cacgcggaac gtcccgagcg gttgccccgc atctgcgccc actgcaacgc cgccgtccga
    22801 gctgcttcgt catatggcac atcaaaatct tccaaccagc tttgcactgc cgacagataa
    22861 tcgttttgat cgaacggata aaagctgagc cacaatccga atcggtcgga caaagatact
    22921 ttttcttcca ccgcttcttt ctgatggatt tctccgcgca cgcctgtcgt acctgcattt
    22981 tcatccaaat attcgggcat caggtgccgt ctgttggaag tcgcgtacac cagcacgttg
    23041 gcgcaacgtt gggacaaacc gccgtccaat gccgttttca atgccttata ggtttcgtcg
    23101 ccgctttcaa atgacaaatc gtcgcaaaac acaatgaatt tttccggacg ttccttcaaa
    23161 agcgtcaaca ggtaaggcag gccgatcaaa tcgcttttat cgacttcgat caggcgcaac
    23221 cccttgtccg catattcgtg cagcagggct ttgaccagcg aggatttgcc tgttccgcgc
    23281 gcgccgctca tcaatacatt gttcgcgggt ctgccgacaa tgaactgttc ggtattacgc
    23341 accagcaatt cggtttgcct gccgactccc gccagccttg ccaaggggaa ggtgtgcgga
    23401 tcgggcaagt gttcccaaaa accttttttg cccgcactct gccagcggaa ggcaagcgcg
    23461 ttccaatccg tatgcccagg ttcgggcgga agcacggcat ccaaacgccg caaaacggca
    23521 taggctttat cgagaaattc gttcaattcc atctctgcct cactttgcat atctttgcgc
    23581 catcagccgt tcgacggtat cgacgattgc ctgcgtattc ggatcgattt cgatgttgat
    23641 cctgtcaccg acctttctgc tgccgaacag cgtccgttcc aaagtttcgg gaatcagatg
    23701 gacattgaaa cggctgtctt cgactttgcc tatggtcagg ctgcaaccgt ccaagccgac
    23761 gaaccctttg gtcaggatat agggtttgag ttcgtgcggg agcgcaaacc aaaccgtgcg
    23821 gttgtacccg tcccgttcga tttcgacaat aggcacggtt gccataatgt gtccgctcat
    23881 gacgtgtccg ccgatttcgt ctccgaaacg cgccgcccgt tcgatgttga cgcaatcgcc
    23941 ttccttcagc agccctaaat tggtttttgc caaagtttcc gccattaaat cgaaactgac
    24001 acggtttcct tcgatttcgg taatcgtcag gcagcagccg ttgttggcga ccgatgcgcc
    24061 gcgttgcaga ttgtccgccg cctcttgcgg aagctcgacg acataagtct gaaatgcctc
    24121 cgacgggcgg tggattgccg tcagttttcc caagccttga acaatgcctg taaacataat
    24181 cctgtttccc tgtgtcggta aaaatggtgc aaattgtagc atctccccgc gaaaaatgcc
    24241 gtctgaaatg ccttcagaca gcattatgcc tccgattcgg gcaaaaaccg cccggtacgg
    24301 cttgaccttt cctttccacg ccggtcggcg gtcttaccct tatccctcct gcaaatcggt
    24361 ttgcgtgttc aagtcggcaa aatgcccgtc aaactcgaat ctgaccgacc gcgccctttg
    24421 ctgctgcaac cagcttctta atgttttcat acccgaaaac agatagggaa tcgcgctttg
    24481 cagaatctgc gggcggatat acataatgtt gtagtgcatc gttatcggcg tttccacata
    24541 atacgcattg cacaacggtg tgcgtttcga cacggtttca aaccttgcca ccaaatcgtc
    24601 cggcagatac ggcatatcgc acggcacaac caaaagccag tcggcagtcg ccaactgcaa
    24661 atcgttggct gcggtacaca atgccgaaag cgggccgaaa tgctgccact gccgtgcgtc
    24721 cggaaaaata tgcggacttc ttcgagcata ttcttccaaa ttccggttgg tgctgatggc
    24781 gatatggctg acctgcggcc tgaccctgtc gatgacatgg tctatcagtg ccttaccccc
    24841 caaaagagca agccccttgt cctcgcctcc catacggctc gcctgaccgt cggccagtat
    24901 cagggcaaaa gttttcattg cggatgttct cttggaaaag ttcgaggttt tcatgattgc
    24961 agtctgccgt tcccaatgac tcaaaatgcc gtctgaagca gacggcaaat aaattcatat
    25021 tatctgaatt ttatcataac atgatttaac actgaagcgg tgcgaattaa gttttcctta
    25081 accaattctt tctgagcagt tgtatctaat tccaaagaat gatattgttt gcattattta
    25141 gaacaatttt tcgccgagca tgatactgcc agcccgtttt tcagacggca tcagcctttc
    25201 cctacgcctg aaactcctga ccggactgtg ggtcgggttg gcggcattgt ctgtcgtttt
    25261 gacactgctg ctctctttgc gtctggaaaa cgcggcctcc gtcatcgaag aggcgggcaa
    25321 cttgagaatg caggcatacc gtctggcata catggcgggt gaaggctcgc cccgtgcgca
    25381 aattgacaat caggttgccg aatttgaaaa aagtttaaaa cgcattgccc aaagcgatgc
    25441 catccatccg ctgattcctt cggacacccc tcttgcttat gatttgatac aatccatgct
    25501 gattatagat tggcaggcac acatcctccc cccgctccag tcctaccggc gaccgactca
    25561 ggtcgatctc taccgctttg ccggaaacat cgaactgttt ttgcaggcat tggaaaatgc
    25621 caacgaaaaa aacacatggt ggctcaggcg ttttcaatgg gcaattatgt tgatgacgct
    25681 ggtgtcgtct gtactgatgc tgttttggca ccagatttgg gttatccggc cgctgcaggc
    25741 gttaagggaa ggtgcggaac gcatcggacg gaggtgtttc gatattccgg ttcccgaagg
    25801 cggtacgccg gaattcaaac aggtcgggcg ttgtttcaat caaatgggcg gcaggttgaa
    25861 aattttatat gatgatttgg aaggacaagt cgccgagcag acacgcagtc tcgaaaaaca
    25921 aaatcaaaac ctgaccctgc tgtaccaaac tacacgggat ctgcaccaat cctacatacc
    25981 gcaacaggct gcagaacatt ttctaaaccg tatcctgccc gccgtaggag cagattccgg
    26041 cagagtttgt ttggacggcg gatccgatgt ttatgtttcc attcatcatg cggattgcgg
    26101 cacagcagct tcggatttgg ggaagtacca tgaggaaatc ttccccattg agtaccagaa
    26161 cgaaacattg ggcaggctgt tgctcagctt tccaaacggc atttctcttg atgaagacga
    26221 ccgcatcctg cttcaaacac taggcaggca attgggcgta tcgcttgccg gcacaaaaca
    26281 ggaggaagaa aaacgcctgc ttgcagtatt gcaggaacgc aacctgattg cgcaaggatt
    26341 acatgacagc atcgcacaag cattaacgtt cctaaaccta caggtacaga tgctggaaac
    26401 cgcctttgcc gaaaacaaac gggaggaagc cgcagaaaac atcggcttca tcaaaacagg
    26461 cgtgcaggaa tgttatgaag atgtccgcga actgctgctc aacttccgta ccaaaatcag
    26521 caataaagaa tttcccgaag ccgttgccga cctattcgcc cgctttacgc aacaaaccgg
    26581 gataacggtc gaaaccgcct gggaaaacgg ttcgttcctg ccgcctcagg aagcgcagct
    26641 ccaaatgatt tttatcctgc aggaaagcct gtccaacatc cgcaaacacg cccgcgccac
    26701 ccatgtaaaa ttcacccttt ccgaacacgg cggacgcttt accatgacca tccaagacaa
    26761 cggacaaggt ttcgacacgg agaaaatagg agaacctacg ggcagccatg tcggactgca
    26821 tatcatgcag gagcgtgcca aacgtatccg cgccgtattg aaaatctatt cccaacccca
    26881 acagggaacc accgtctcat tggcgggtgt atctgaagaa agcttgaaat gaccattaaa
    26941 attatcctga tagacgacca taccctcttc cgcagcggca ttaaagccct tttgtcgcgc
    27001 caacacggtt ttgaagtcat cggcgaagcc gcagacggcc tctcgggcat caaaatgatt
    27061 agtcggctgc aacccgatgt cgtcctgctc gatttggata tgcccggcat gaacggacgc
    27121 gaagcactct cccaaatcat cagcatcaat ccgcagcagg cagtgattat gctgaccgtt
    27181 tccgaagaca gcgacgactt gaccgaatgt attcgcatcg gcgcgcgcgg ttacctgctg
    27241 aaaaacatca acgctgactt tctgctcgaa agcatacgca aagtcgccga aggcgataat
    27301 gtattctcgc ccgagatgac cgccaaactc gttaaaagcc tgatttcccc ccaacctgcc
    27361 caagggacac aggcactctc ctcacttacc cctcgcgaac tggaaatctt gggctatctc
    27421 gccgcaggac acagcaacaa aatcatcgcc cgccacctcg atcttgccga atccaccgtc
    27481 aaagtccacg ttcaaaacct gctccgcaaa ctcaacctca gcagccgggt gcaggccgcc
    27541 gtttacgcca tccggcacaa cgtcccccaa cccgtgctgg aataaggcgg gttcagacgg
    27601 catattaggg gttttaatcc ccgtacggtc attcggataa cagaccacgc atgtaagctt
    27661 atgcccccat aagtacgctt ggcatagcag taatattgtt cggtttagtg ttttccgttt
    27721 gcccctatct gatactgcaa tatcagctat gccgtctgaa aacgcatcat catgatattt
    27781 tcagacggca taataaaaag cggaaatact aatgcagggt aaaatgttcc attccgaatc
    27841 ccataaatat acaatggctt atatcgttta agcatgtgta acccaccctc atatcaatca
    27901 a
//
LOCUS       DAWHRA010000007        27176 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWHRA010000007 DAWHRA010000000
VERSION     DAWHRA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.007"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(467..1222)
                     /locus_tag="WIB30_000242"
     CDS             complement(467..1222)
                     /locus_tag="WIB30_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ3796384.1"
                     /translation="MKYIRLFFLCTALISTNAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYILHNLGFRCTSR"
     gene            complement(1349..2509)
                     /gene="nirK"
                     /locus_tag="WIB30_000243"
     CDS             complement(1349..2509)
                     /gene="nirK"
                     /locus_tag="WIB30_000243"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ3796385.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAASAEAASSAAQAAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMTMEDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAIAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASAPAKSDY"
     gene            2880..5135
                     /locus_tag="WIB30_000244"
     CDS             2880..5135
                     /locus_tag="WIB30_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ3796386.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSTWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFNFFFWTSWSASTNRPDENFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPELNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            5314..5847
                     /locus_tag="WIB30_000245"
     CDS             5314..5847
                     /locus_tag="WIB30_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ3796387.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5910..7094)
                     /locus_tag="WIB30_000246"
     CDS             complement(5910..7094)
                     /locus_tag="WIB30_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ3796388.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDAQRVADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNGGYAVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(7134..7538)
                     /locus_tag="WIB30_000247"
     CDS             complement(7134..7538)
                     /locus_tag="WIB30_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ3796389.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEIVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            complement(8145..8582)
                     /gene="rnhA"
                     /locus_tag="WIB30_000248"
     CDS             complement(8145..8582)
                     /gene="rnhA"
                     /locus_tag="WIB30_000248"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ3796390.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTASKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            8696..9565
                     /gene="tehB"
                     /locus_tag="WIB30_000249"
     CDS             8696..9565
                     /gene="tehB"
                     /locus_tag="WIB30_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ3796391.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSMYCTAADYFH
                     KKYGMSATHSAVVAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGSMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(9615..10421)
                     /gene="thiD"
                     /locus_tag="WIB30_000250"
     CDS             complement(9615..10421)
                     /gene="thiD"
                     /locus_tag="WIB30_000250"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ3796392.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCKAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            10482..10679
                     /locus_tag="WIB30_000251"
     CDS             10482..10679
                     /locus_tag="WIB30_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796393.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            complement(10899..10974)
                     /locus_tag="WIB30_000252"
     tRNA            complement(10899..10974)
                     /locus_tag="WIB30_000252"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10938..10940),aa:Glu,seq:ttc)
     gene            complement(11694..13106)
                     /gene="trkA"
                     /locus_tag="WIB30_000253"
     CDS             complement(11694..13106)
                     /gene="trkA"
                     /locus_tag="WIB30_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ3796394.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(13204..14727)
                     /locus_tag="WIB30_000254"
     CDS             complement(13204..14727)
                     /locus_tag="WIB30_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ3796395.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMNVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            complement(15034..15840)
                     /locus_tag="WIB30_000255"
     CDS             complement(15034..15840)
                     /locus_tag="WIB30_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ3796396.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDTAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(15892..16143)
                     /locus_tag="WIB30_000256"
     CDS             complement(15892..16143)
                     /locus_tag="WIB30_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796397.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            16243..17004
                     /locus_tag="WIB30_000257"
     CDS             16243..17004
                     /locus_tag="WIB30_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796398.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIESGENADEAESGTNEPAVSENGENDAEETLMPV
                     QLNILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            17149..18318
                     /gene="metZ"
                     /locus_tag="WIB30_000258"
     CDS             17149..18318
                     /gene="metZ"
                     /locus_tag="WIB30_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ3796399.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAGYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            18414..18620
                     /locus_tag="WIB30_000259"
     CDS             18414..18620
                     /locus_tag="WIB30_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ3796400.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(18718..18830)
                     /locus_tag="WIB30_000260"
                     /pseudo
     CDS             complement(18718..18830)
                     /locus_tag="WIB30_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(19950..20702)
                     /locus_tag="WIB30_000261"
     CDS             complement(19950..20702)
                     /locus_tag="WIB30_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3796401.1"
                     /translation="MTDILIDNTATEAVRALIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAKPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNSAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
     gene            complement(20806..21273)
                     /locus_tag="WIB30_000262"
     CDS             complement(20806..21273)
                     /locus_tag="WIB30_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ3796402.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            complement(21325..22842)
                     /locus_tag="WIB30_000263"
     CDS             complement(21325..22842)
                     /locus_tag="WIB30_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ3796403.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQALRIPSFSAVADITMIGVLTYLF
                     GGINSGFGILILPFVGSSCLLSYGRYPLLYASYAAILLILNALADSNINMYPLILDAK
                     TVTNTFVLVTGSYFVAMIASLSVRYIDRAGKLAHENHAAYRRIRGLNQIVLNRVQEAV
                     VIINVEYQTVLFNKKAKDLLPTLEIGQHTALFDPITVLWDKAPSRTFERNIDTPALTA
                     RIRAVPMNKEQNKLLILYIRPQSEIQAEALSVKLAALGQLTANIAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAAGCIRLDMQGNHLTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            complement(22863..25166)
                     /gene="parC"
                     /locus_tag="WIB30_000264"
     CDS             complement(22863..25166)
                     /gene="parC"
                     /locus_tag="WIB30_000264"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ3796404.1"
                     /translation="MNTQAHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPPHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESLTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            25314..25997
                     /locus_tag="WIB30_000265"
     CDS             25314..25997
                     /locus_tag="WIB30_000265"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ3796405.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            complement(26070..27014)
                     /gene="tehA"
                     /locus_tag="WIB30_000266"
     CDS             complement(26070..27014)
                     /gene="tehA"
                     /locus_tag="WIB30_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEZ3796406.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAAASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYVGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GVLSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRS"
BASE COUNT         5995 a         6760 c         7773 g         6648 t
ORIGIN      
        1 tttagaacaa cagcaatatt caaagattat ctgaaagtcc gagattctag attcccactt
       61 tcgtgggaat gacgggatgt aggttcgtag gaatgacgtg gtgcaggttt ccgtgcggat
      121 ggattcgtca ttcccgcgca ggcgggaatc tagactttag aacaacagca atattcaaag
      181 attatctgaa agtttgagat tctagattcc cactttcgtg ggaatgacgg gatgtaggtt
      241 cgtgggaatg acgaaaagtt gcgggaatga cgaaaagttg cgggaatgac gaaaagttgc
      301 gggaatgacg aaaagttgcg ggaatgacgg ttcgggcatt ccttaaatta cccgtgtatc
      361 gctgtaaatc ttagagatgg cggaatatag cggattaaca aaaaccagta cggcaaggcg
      421 aggcaacgcc gtactggttt ttgttaatcc actataattg aaggggttat cggcttgtgc
      481 aacggaagcc caagttgtgc aagatatatt tggattgcag gctggtacgg atgccgtagc
      541 ggaggaaggc ggcatagttg gacgagtcgc tcgacccgat agacgcgcca ctgcaaaaca
      601 tttgcgcgtt ggcattgccg gaagaaagca ggctgctgtt gaaatcttcc gtccattccc
      661 aaatcagacc gtgcatatca taaacacccc agtagttcgg gcggcctttg ccgacatcgt
      721 gcaggccttt ccgtccgccg tcggcatacc aatcgagaat agtgcggttg tagccgggtt
      781 cgtttgagcc gtttttctgc gtggcggaag caagtccggc aaattcccat tcgtcaatgg
      841 tcggcaggcg tttgccttgt gcggcgcaat aggcgttggc ggcaaaccag gaaacattgg
      901 ttaccggttg ttttaattcg cccgccttcg gcgcatagct gcggctgccg tttttcatcc
      961 aatgcttcag gtaagcgggt tctgcctgtt tggaaccgat cctgcctttt tgccattggg
     1021 ggtggctgtt gacaaattcg gcaaactcgg cattggtaac gggatattta tccagtttga
     1081 acggtttgac tttaatcaga ccggtatctt ttttcagata aagcgggcgg tagctgccgc
     1141 cttcgatttg aaccatttcg gcagctgccg cattggtact gatgagtgcc gtacagagaa
     1201 aaaataaccg gatgtacttc atatcatgcc tcctgacagg cagttaaaat caatcttccg
     1261 aaaggaaaga ttggttgtta aaaaaccact gccgtgcgta atgaagtacg gcggcagtgg
     1321 ttcgtttgcc taaaaatggg tgttcaattt agtaatcgct ttttgcagga gcagaggctg
     1381 caggagcaga agctgcggga gcggaagcag caggagctgc accgttaccg gcgtaagcgg
     1441 tatcactcaa tttttgagtc atgatttcag ggttttctgc accctctact ttcaattgac
     1501 ccagtgcgcc tttgttgaat gcgcggaaga tagagtggtc aaccaaagtg tagctgcccg
     1561 ggatgtcgac tttgaattcg acgatggcag agccgccggc aggaacgatg gtgctttgta
     1621 cgttttcgtt aatcagtttg ccgccttcca cataaacttt gtcgaagatt tcaccgatta
     1681 cgtggaagga agacaccaag ttaggaccac cgttaccaac gtacatacgt acagtttcgc
     1741 ctgctttggc tttcagggcg ttgtcgccgg cgatagcacc tacgtgaccg ttaaatacaa
     1801 cgtattcagg ttgttcggcg atggctttgt ccatatcgaa cggttgcagg ccttgcgcgc
     1861 cttttttgcc tttggtgtag aagtcgcctt ggacgatgta gaactcttta tccactttcg
     1921 gcagaccttc tttaggctcg accaaaatca gaccgtacat accgttggcg atgtgcatac
     1981 cgaccggcgc gacggcgcag tggtagatgt acaggccagg ttgcagggct ttgaagctga
     2041 atgtggaagt acggcccgga gcggtaaagg ttgcagccgc gccgccgccc tgaccggtag
     2101 ccgcgtggaa gtcgacgttg tgcggaacgg tagaagaagg attgttggag aattccactt
     2161 caaccgtatc gccttcgcgt acgcggatca tgcggcccgg aacatcgccg tcaaatgtcc
     2221 agtagcggta ttccacaccg tcttccatgg tcatggtttt ttcgacggtt tccattttca
     2281 cgcggacttt ggcagggtag tcgcggtcga ttgcgggagg tacttcagga gcgtgggtgg
     2341 taactgcatc gatgacgggc agttcgcctg caggcgtttc ggcggcagct tgtgcggcgg
     2401 aacttgcggc ttctgcggaa gcggcagggg cttgcgcggc aggttcgccg ccgcaggcgg
     2461 ctaatgcgaa taaggaagca atcattgcag ctaaggcttg gcgtttcata atattttcct
     2521 tttgtaagaa aagtagaggg attggttaag aacttggtgt gattatgcgt ttgccccatt
     2581 gggggcattt tgatttaagt caaataattc ataaaatatg aacttaaaat gatacttcta
     2641 atactttata gctactaaaa attacaataa ataggctgat ttgcttaagg gcttcggcaa
     2701 atcaggcaaa ggcagggtag ttgccgacta aagaataatg atagttatta tcatatatgt
     2761 atttgtttta tatgtgagtt tctttgaaca aatgtttatt tgcgcggtaa accgtgctac
     2821 aatctttaac attcatattt tgtgaatttt aatccactat attaaaagga gctctcaaaa
     2881 tgggacagta caagaagctg tggtacttgc tgtttgccgt tctggcggta tgctttacca
     2941 ttcttggcta tatgggcagc gaggtttata agaaagcccc gccttacccc gaacaggtcg
     3001 tttccgcatc aggcaaagtg ctgatgacga aagacgatat tttggcaggt cagtctgcgt
     3061 ggcagagtac cggcggtatg gaagtcggtt cgattctggg acacggcgca tatcaggctc
     3121 cggactggac ggccgactgg ctgcatcgtg agttgtccac ttggttggat ttgaccgcgc
     3181 aacagactta cggcaaaaaa tttgatgaag tttcccctga agaacaagcc gttctgaaaa
     3241 cccgcctggc tgacgaatac cgcaaccaaa gccgtgtgaa agaagacggc agcgtcgtca
     3301 tcagcgatac gcgcgtaaaa gctatcgaaa gcatcctgcc ttactaccac ggtgtttatg
     3361 gcgacgatcc caagctgcaa accactcgtg aacactttgc aatgaaaaac aatacattgc
     3421 caagtcaaga ggcgcgtgaa aaactgttca acttcttctt ctggacttct tggtctgctt
     3481 cgaccaaccg tcctgacgag aattttactt acaccaacaa ctggccgcac gaacctttga
     3541 tcaacaacgt accgaccact gaaaactata tgtggtcatt caccagcgtt gttcttctgc
     3601 tgatgggcat cggtttgttg atgtggggtt attccttcct gaccaaacac gaggaagtgg
     3661 aagtgccgtc tgaagacccg atttccaaaa tccaactgac cccttcgcaa aaagcattgg
     3721 gcaaatacgt cttcctgacg gtcgccctgt ttgtggtaca agtattgctg ggcggtctga
     3781 ccgcgcacta caccgtcgaa ggtcagggtt tctacggcat cgatgaggcg ttgggctttg
     3841 aaatgtccga ctggttcccc tatgccctga cccgtacttg gcacatccaa tccgccatct
     3901 tctggattgc aaccggcttt ttgacggcgg gtttgttcct cgcgccgatt gtcaacggcg
     3961 gcaaagatcc caagttccaa cgtgcaggcg tgaacttcct ctatatcgcc ctcttcatcg
     4021 tggtcggcgg ttcttacgcg ggcaacttct tcgcgttgac gcacatcctt ccccccgaat
     4081 tgaatttctg gttcggacac caaggttacg aatacctcga tttgggccgt ttctggcaac
     4141 tccttttgat ggtcggtctg ctgttatggc tgttcctgat gttgcgttgc acggtttccg
     4201 ccttcaaaga aaaaggcgtg gacaaaaacc tgttggctat cttcgtagct tctatggtcg
     4261 gcgtgggcgt gttctacgcg ccgggtctgt tctacggtga aaaatccccg attgccgtta
     4321 tggaatactg gcgttggtgg gtggttcacc tgtgggtaga aggcttcttc gaagtatttg
     4381 ccactgccgc ctttgccttt gtcttctaca atatgggctt tgtccgccgc agtaccgcca
     4441 ctgcctccac tttggccgct gccgccatct ttatgttggg cggcgttccg ggcacgctgc
     4501 accacctgta cttctccggc tctacctccg cctctatggc aatcggtgcc tgcttctccg
     4561 ctttggaagt cgtgccgctg gtgttgctgg gccgtgaagc atacgaacac tggtcttacc
     4621 aacacctgtc cgactgggcg aaacgcctgc gttggccgct gatgtgcttc gtggcagtcg
     4681 ccttttggaa tatgatcggt gccggcgtat tcggcttcct gatcaatccg ccgatttccc
     4741 tgttctatat ccaaggtttg aacacttctg ccgttcacgc gcacgccgcc ttgtttggtg
     4801 tgtacggttt cttggcattg ggcttcgtat tgctggttgc ccgttatttg aaaccgaacg
     4861 cgcagtttga cgacaaactg atgacttggg gcttctggct gctcaacggc ggcttggtcg
     4921 gtatgattgc catcagcctg ctgcctgtcg gcgcgattca ggcatacgcc tccatcacgc
     4981 acggtctgtg gtatgcccgc agcgaagagt tcctgcaaat ggaaatcctc gataccttgc
     5041 gttgggtacg tacggcagct gacttggtct ttatcggcgg tgcaatctgc gttgccatcc
     5101 aagctactaa aatcgtattc ggccgcgaca aataagtttt gcggtatgaa atgagcaact
     5161 gcacccgttt tcgggtgcag ttttttattg cggattggga cgggaatgga aaaggatgtg
     5221 gattttttat tttaatcggc tatacaattg ctgctttcat gccgtctgaa gcacgtttca
     5281 gatggcatcg gtttacatca gataaggata aaaatgggta tttacgattt tcaaatgaaa
     5341 gatgcggaag gcaatgcggt tgatttgtca ggctatcgcg gcaaggttct gctgattgtc
     5401 aacacggcaa cgcgttgcgg tctgaccccg caatacgagg ctttgcagaa gctgtatgca
     5461 caatataccg cagaaggctt ggagattttg gattttccgt gcaaccagtt ccgcgaacag
     5521 gctcccgaaa gcagcggaga aattgcccaa gtgtgtatga tgaagtttgg cacgaagttt
     5581 aagattttcg acaaaatcga agtcaatgga gcaaataccg cgcccttgta tgcctacctg
     5641 aaatccgtca aaccgcaaga taaaggcaat catctgttta aagatttcgt gttgaagctc
     5701 gcagctttgg gagagaagcg cgacgaaggc gacatcaaat ggaactttac caaattcctc
     5761 gtcaaccgcg acggcgaagt ggtcgagcgt tttgcgccta gtgttacgcc cgaagaaatc
     5821 gaagccgata ttcgggcatt gctgtaatct gccgccgtct tgggaaatgc cgtctgaagg
     5881 cttcagacgg catttgtccg tttgcgggtt tattccgaaa tatcggcaac accgctttcg
     5941 gggaagatag gcaaatccgg cggcgggagc agggtatcca aagtcagggc ggacgcatcg
     6001 agtgtggggt agccttcaaa cgcgaccgca taaccgccgt ttccattcat acggattcgg
     6061 gcgtgcgcgc ctagagggaa agtgattttg tcggcaatgt gtccgaacgg gaagcccgtc
     6121 agcacgggga ttttcgccgt gcgcgaaaca tggttggcaa cggcagaaaa atcataagac
     6181 ggatcataga catctcgaat tttttccata cggaaattgc cgaacacgat ggcgcgctgt
     6241 ttcttcaaaa tacccgaaag atacagcgta ttgagcatac gttcgatgcg gtagggctgt
     6301 tcgccgacat cttcgaggaa caaaatgccg ccgtcgatgt cgggcatata aggcgtgccg
     6361 gcgagcgagg cgaggacgct taagttgccg ccccacaata tgccttcggt ttcgacatcg
     6421 gcgcgttgga tataaggaac atcaacggtc aggcggtttt gggttgcacc cttgataaag
     6481 gcatccatcg taaacgcacc gggggcgggt ttgccaaaat cgctataagc catcgggccg
     6541 gcaaaactca tcatattgcc ttttgccaac aatgccagct ggacggcgca tacgtcgctg
     6601 aatccgaaaa acagcgtgcc gtgttcgcgc atccttgcgc cgagcgaagc aaaatcgata
     6661 tgcggcagaa tccgcgccgc accgtaaccg ccgcgcaagc ccatcagcac tttaggcgtg
     6721 gcgacgcggc cggaagcgac ctcttggaaa tcggcgacac gttgtgcgtc cgtaccagca
     6781 aaccgttgga agcggcggct gcccgcctgt tggttggtta cggtaaaacc gacattgtaa
     6841 aggcgggtta aggctgtgtt gacgcggttg gtgtcttcgg caaatcctga cgaagcgaca
     6901 acgcgcagaa ggttgccgtc cgaactttga cggcgtggcg tttggagagg cacggttcgg
     6961 gctttcacaa cggaatggga agagggaagg ggcggaacgg atgtggcgga tgtgccgcaa
     7021 gcctgaagca gccccgcgcc ggcagcggcg gtgcaggttt tcagaaaacg gcggcgggag
     7081 gtcggttcgg tcataatttc cctttcatac ggtttcagac ggcatcggcg ggcttaaagc
     7141 agctttttaa gctgttccgt ccaatcttgc ggcagggtca taccgtgttt ccggacgggg
     7201 tttgcccaaa tcggcgcggg gaaggacgca tcgttttcaa agcgggcgat aatatgccag
     7261 tgcaggtgcg gcacgacatt gcccaagctg gcgaggttga tttttgccgg ccggaacact
     7321 tggcgcatag cggcttcgac tttgtacact atttccatca attcgccgcg ttccgctgcc
     7381 gaaaggtcgg tcatttcggc aatatgctta cgccaaatga cgcggcagaa tgcaggcgaa
     7441 ccgctgtcgt tatggacggc gatgacgcgc agcttggggg tttgcagcaa aatatcttca
     7501 tcttgtgccg tgcagatggg gcagggttgg gcggtcattt ttccttattc ctgaaagtgt
     7561 ttaaaaaacg gtagggtaat ttttaatatt tcaatctgtc tgtccgcatt gccgttaatt
     7621 gtaccacttc atagggcggt gctctatgtg tcgtcctgtg tgttgaaaca tatcggacgc
     7681 gtttgaacag ttgtgcggca aactgtttta gcggcaggca agagatgccg tctgaagccc
     7741 attgtccgtc attcccacac aggcagttgc tacggttgct gaagcccctt agccgtcgcg
     7801 caggcggttg ctacggttac taaaatcccg tcattcccgc gcaggcggga atccagtccg
     7861 ttcggtttca gttgtttctg ataaatcttg cagcgttggg tttttagatt cccgctttcg
     7921 cgggaatgac ggggcggtcg gatgccgcct gaaactccgt cattcttgca acttttcgtc
     7981 attcccgtga aaacgggaat ctagaacttc aaactttcag ataatctttg aatattgccg
     8041 ttgcccgacg gtttggattc ccgcttgcgc gggaatgacg gtggaggacg atgccgtctg
     8101 aagcccatta gcggtttcag acggcatttt tgccggagcg gcagtcagga aaactgcgct
     8161 gcgccacggt ttgccaaatc gtcggcgcgt tcgttttcgg cgtgtcccgc gtgtcctttc
     8221 acccaagtcc aactgacttg atgccgtccg actagagcgt cgagttcttt ccacaagtcg
     8281 tcgtttttga cgggctgttt ggaggcggtt ttccagccgt tgcgcttcca accgtgtatc
     8341 cagttttcca tgccattttt gacgtattgc gagtcggtgc agatgatgac ggtgcagcgg
     8401 cgtttgagcg atttcagtcc ttcgatgacg gcagtcagtt ccatgcggtt gttggtggtt
     8461 tgcgcttcgc cgccgaaaag ttctttttcg tggctaccgt agcgcattaa cacgccccag
     8521 ccgcccgcgc cgggattgcc tttgcacgcg ccgtcggtgt aaaggtaaac ggtttggtcc
     8581 attattgtgc ctttgtgtgg tgtccagcgt gattataagc ggtttgtgcg ccgtgccgtc
     8641 tgaaagattg cggcggatta aaataggcat ataatgtgca actttatttt tgaatatgaa
     8701 ggagcggata gtgggtcaaa gcggcgagtt gttttgtttc ggacagatgc ccgtatggaa
     8761 ggcggaaaac ctgccggaaa ttttgttgtc gggttattcg tccgaaggag gggaatgggt
     8821 ctgcctgaat gtgttgcagg gcgatgtcga agtccgcacg caggacgggg cggcagaggt
     8881 ttggtcggcg gaaagcggcg attgcgtgtt tgcgccgcag caggtgtttt cggtcaaacc
     8941 gaaaacggat gatgctgaaa tccgtttgtc gatgtattgc acggcggcag actattttca
     9001 caaaaaatac gggatgagtg ccacgcattc tgcggtcgtg gcggcacagg ataccgtacc
     9061 ggcgggcagg gcgttggata tgggctgcgg acaggggcgc aacgcgctgt tcctcggctt
     9121 gaaagggttt gacgtaaccg ctgccgatca caatccggcc gcgttggcaa acgtggcgga
     9181 gctggcagag gcggagggtt tgaacgtccg cacgctggaa tatgatttga acgccgccgc
     9241 cttgcagggc gaatttgatt atattgtggc aacagtggtg ctgatgttcc tgatgccgca
     9301 gcgcgtgcct gacgtgattg ccgatatgca ggcgcatacg gcggcgggag gatacaattt
     9361 gatcgtatcg gcaatggata cggcggattt cccctgtccg atgccgttcc cttttaaatt
     9421 taaagagggc gagctgaagg attattatcg ggattgggaa ctggtcgaat ataaagaaga
     9481 attgggttct atgcatgcca aagacgaaaa cggcaatccg atacggttta aatttgtaac
     9541 gatgctggcg aaaaagcctg aataatcctc aaaaatgccg tctgaacatt ttgtttcaga
     9601 cggcattttt acgtctagtc ccgatacgcc caatgattga ccgggccgtg tcccgcgccg
     9661 atttccaaag ggtttgagat tgccgccgta atgtaggact tggcagtccg tacggctttg
     9721 caaacgtccg agccttttgc caactcggcg gtgatgcagg cggaaaacgt gcagcccgtg
     9781 ccgtgcgtgt gggcggttgg aaagcgcggg ctgtccaatt ccagcgtttc attttgcgta
     9841 aacagccaat ccgtgcagcg tccgcttgtg ctgccgttca aatgtccgcc tttgataacg
     9901 acatttttga caccgtaatc aagcaggatt tttgccgcac gttccgcatc tttacggttt
     9961 tcgatatgca cgccggtcag ggcttccgcc tcgggcaggt tgggggtcaa tacatccgta
    10021 tcgggaagca gcaggcgcgt cagtgccgca acggcggaat cctgcaacag cggcgcaccg
    10081 cctttggcaa tcatcacagg gtcgagtacg cgcctgccaa agctgcagtg tttcagcttg
    10141 tcggcaacgc attcgatgat ttccgccgtg ccgagcatac cgattttgta ggcgcggatg
    10201 tcgaagtctt ccctgattgc ttggatttgt gcggtgatgg tttcggtcgg gacgagatga
    10261 accgccgaca cgcccaaggt attttgcgcg gtaacggcgg tgatgacgca cgttccgaac
    10321 acgccgcgca tctgaaatgt tttcaaatcc gcctgaatgc ccgcaccgcc gcccgaatcc
    10381 gaaccggcga tggtaagcgt ttgcacaaaa gagcttttca tttgttcctc cttaatattg
    10441 cgtccgataa aaagggctaa tgcctgtaaa aaagcacggc gatgccgtct gaaaaccgca
    10501 gcgcgggggt ttgtggtgca ttatatgccg tattgcagga caaggcacag ggcagggcgg
    10561 cggggtgcat cgtagcaact gtatttttgc ccgacggggt gaaaaataca gttgctacac
    10621 ccactgataa tgaaaaaaga gagcaatgcc gtctgaaccg cgtgttgcgg cgagcataaa
    10681 ccggcacgct ttattcgtca tttccacaaa aacagaaacc taaaattccg tcattcctac
    10741 gaaagcggga atccagacct gtcggcacgg aaacttatcg gataaaacgg tttcttcaac
    10801 cctacgctct agattcccgc ttttgcggga atgacgaaaa attgcgggaa tgacgactca
    10861 taataaaaaa cctgcttgaa acaagcaggt ttttgaattg gcacgcccac ggggattcga
    10921 accccggttg ccgccgtgaa agggcagtgt cctaggcctc tagacgatgg gcgcgtggaa
    10981 acggacatta tacatactta tagaaattta gcaatacgat gccgtctaaa caaatgtcag
    11041 gggggcgttt gtctgatcgc atcacaatat aaaaacatat aaaaaacatt ggaatatcaa
    11101 gcaattcatt tttttgcgtg gtaatgccga caacggaagg cagctttatt ttgaggcgca
    11161 aaggtcttcg atgcacacga tgcgctagac ttggcaggtc aatatgggcg tgcgcagtat
    11221 gttctgatgc ggcggattcg cctgaaaaca agccggaacg gtcagatgcg ttccggattg
    11281 tttttttgtc gggggggtgc cgccatatca aaacaggcgg gcgatgccgt ctgaaacttc
    11341 gtcattcccg cgaaagcggg aatctagaac ttttaaattt tcagacggct tttgaatatt
    11401 gccgttgccc gacgttctgg attcccgcaa cttttcgtca ttcccgcgaa agcgggaatc
    11461 tagaacctct aaattttcag ataacctttg aatattgccg ctatcccaag gtctggattc
    11521 ccgcctgcgc gggaatgacg ggtcttttat aacctttgaa tattgccgct atcccaaggt
    11581 ctggatgccc gcttacgcgg gaatgacggg gagggcggat gccgtctgaa gcccacctac
    11641 cattatttcc gcttccatac cgcttggcgg caatatgtcc ggaatggggc ggtttatccg
    11701 aaaaagccca ttttgacctg gatgagtttt tccagttcgt tcaggatgcg ccggcgcgag
    11761 acgaaaaaga tgatgtggtc gccgtcttgg atgacggttt cggtatggtg tcccataatg
    11821 gtttcgccgg ttccggcgcg gacgacggcg gcaatgtggc agccttcggg ccatttgatg
    11881 ccgctgatgc gcctgccgat gatggcggaa gtttttttgt cgccgtgtgc gacgacttcg
    11941 atggcttccg ccgtgccgcg ccggatgggg tggacggcaa cgatgtcgcc gcgccggatg
    12001 tgggcgagta tcgagccgat ggtgatgagg tggggggaga cgacgatgtc gattttgttg
    12061 ccttcgagca aatcgacgta gcttgagcgg ttgacgatgc cgatgacgcg cttcgcgccg
    12121 aggtttttcg ccaaaagggc ggacataatg ttgctttcgt cgtcgttggt cagggcgcag
    12181 aatacgtcga tttcgtcgat gtattcgttg tcgagcaggg tttcgtcggt tgccgaacct
    12241 tgcaggacga gggtgttgtc gaggttttcg gctatccatt cggcacggcg cggccggcat
    12301 tcgatgattt tgacgttgta tgcgtgttcg agctgcttgg cgagacggta gccgatgttg
    12361 ccgccgccgg caatcatgat gcggcgggtg ctggtttctt tggggcgcaa ttcgggtatg
    12421 accgcgccga tgttttcggc ggcggcggca aataggattt cgtcgccttc gatgatgacg
    12481 gtttgcggcg cggggacgat gaggcggttg ttgcggtaaa cggcgcagat ttggcagtcg
    12541 gccccgtcgg gcaaatcttg ggcgatgtcg gcaatgctgc gtccgacaag cagtccgccg
    12601 cgccgcgcct gtatgatgac catccgcacg cggtcgtctg caaaacgtaa aacctgcaat
    12661 gcgcccgggc agtctatcag gccggcaagc tgttcggtaa cgagctgttc ggggctgatg
    12721 gtttcggtta tgccgaatat ggaaaggctg ccgttttcgt tgttttcgag cttggggctg
    12781 aggtattcga ggtattcgct ggaacggacg cgcgcgatgc ggccggggat gttgaacagg
    12841 tcggcggcaa ctttgcaggc gacgatgttg gtttcgtcgc tgcgggagag cgcgagcagc
    12901 aagtcggcat cttccgcgcc ggcgcgttct aatgtgaagg gggatgcgcc gttgccgaaa
    12961 acggtttgga catcgaggcg gctgcctgtt tcctgcaatg ctttttcgtc gatgtcgata
    13021 acggttacgt cgttgttggg tatggcggca aggttttgtg cgacggtaga acctacctgt
    13081 ccgttgccta aaatgaggat tttcacggtg tgggtcgccg agtgatgaaa tggtggattg
    13141 taccgcaacc gggggcggaa atgccgtctg aaggcagatt ttgtgttcag acggcatggc
    13201 gcctcaagat tcgacgggga tgatgccgat tttcgcctgc catttgcgcg gggcggtggc
    13261 gtggatggat tcgcctttgc tatctacggc gacggttacg ggcatgtctt tgacttcaaa
    13321 ttcgtaaatg gcttccatgc ccaattcggg gaacgccaag actttggaag atttgatggc
    13381 ttttgccacg agatacgccg cgccgccgac tgccatgagg tacacggctt tgttgtcggc
    13441 gatggcttcg caggtggcca cgccgcgctc ggatttgccg atcatgccca agaggtcggt
    13501 ttgttcgagc atttggcggg tgaatttgtc catgcgggtg gctgtggtcg gacctgccgg
    13561 accgacgact tcatcgccga ccggatcgac ggggccgacg tagtaaatca ggcggttggt
    13621 gaaatcgacg ggcaattctt cgcctttgtt gagcatatcg acgaggcgtt tgtgtgcggc
    13681 atcgcggccg gtgaggattt tgccgttcaa cagcaatacg tcgccggttt tccagcttgc
    13741 cacttcttct ttggtcagct tgtcgacatc gacgcgtttg ccgttgtcgg ggctgtaagt
    13801 caaatcgggc cagtcttcga cgcgcggcgg cgtgagttcg acagggcctg agccgtccaa
    13861 ttcaaattcg acgtggcggg tggcggcgca gttcggaatc atggcaatcg gtttggaggc
    13921 ggcgtgggtc ggataatcga ggattttcac gtccaacacg gtggtcagtc cgcccaagcc
    13981 ttgtgcgccg atgcccagcg cgttgacttt ttcaaagagt tcgaggcgca gggcttcggt
    14041 ggtggacaat tccgcgccgg acgcggcctt ttcctgcaat tcttgaatgt cgatgtggct
    14101 catcagggac tcttttgcca tcagcacggc tttttcgggc gtgccgccga tgccgatacc
    14161 caagatgccg ggaggacacc agcccgcgcc catggtcggg atggttttca atacccaatc
    14221 gacgatgttg tcggaaggat tgagcatggc gagtttggat ttgttttcag agccgccgcc
    14281 ttttgccgcg caggttactt cgactttacc gcccggcacg atgctcatat ggatgacggc
    14341 gggggtgttg tctttggtgt tttggcgttt gccggccgga tcggcgagga cggaagcgcg
    14401 cagcgtattg ccttcccaag tgtaggcgcg gcgtacgcct tcgttaacca tctcttccac
    14461 gctcatgtcc gcatcccatt ggacgttcat accgactttg aggaagacgg ttgcgatacc
    14521 tgtgtcttgg cagatggggc ggttgttttc cgcacacata cggctgttga ccaaaatctg
    14581 cgtcatcgcg tctttggcgg caggattttc ttccttctgc cacgccttat aaagcgcgtc
    14641 gatgtagtct ttgggatgat agtagctgat gaattggaag gcatcgcaaa tgctttggat
    14701 aaagtcttcc tgtttgatga cggtcatgat tgtgttcctt ttcaaggttg tggcggtaag
    14761 tgtctgctgc tgttttaaac aacctttttg aaggctgttt tttatcaggt tgaaatagtg
    14821 ccagcgtttg tacggtaaag acaatacata attctaatct cttgattatc aatttgtttt
    14881 aataaaatac tgttttactc ggattttgat atttgtaatc tttgcctgtt gctttgcggt
    14941 agggagaaat cagcaggcag ttcattgtag gccgattgat gaacaatgcc gtctgaaagg
    15001 gcttcagacg gcattgtgtt cggacgggcg ggcttatttt gcggcctgtc cgtcttcttt
    15061 tgcaaaatat ttggcgtaaa tcttgtcgta ttcgccgctt tcgcgtactt tttccaacgc
    15121 atcgttcagc attttgacgg ttgcttcgtc gcctttgcgt accgcgatgc cgtagtgttc
    15181 ggtggtgaag tcgggcaggg taacgaagtc catccctttg gccggattgt ttttcacata
    15241 attggcgatg accgcgctgt cgctgaccac ggaatccaag ccgccgtttt ccagttcttt
    15301 gataatcagg ggaacgtttt caaagcgcgc gattttcgga ttgtcgttgc ccaagagttt
    15361 ggatacggag aaatcgcccg tgtagccggt taccacgccg actttgttca tgtttttcaa
    15421 atcttcggaa gaagatactt ttttgccttt cggaacgagg acgacttggg tgatttcaaa
    15481 atacgggtcg ctgaagtcca tagactgttt gcggtcgtcg gtaatggtta cgcccgacat
    15541 cacaacgtcc gcatcgccgt tgtttaaggc ggggaaaagg ctgtcccacg gctggtgttt
    15601 gaattcgatt ttaaaattgc ccgccttcgc catcgcgttc atcaaatcca catcgaaacc
    15661 ttcgacattg cctttcgagt ctaaagattc aaagggggca aactcggcgt tggaagccac
    15721 gcggtacact ttgtcggggt tggcggcagg cgcggcggta tctttgccct gaccgccgca
    15781 ggcagacagc gcgagcgcgg aacaggcaag ggcggcggca atccattttt tcatattcat
    15841 caaaattcct ttcggtaatc aaaaagccta aaaaatcagt ttaacataaa attaacgttc
    15901 tttttcacgg aaaaacgcat aagtaacgtc gtggaacaaa tccgtatgcg ggctgtcctc
    15961 atagctcaat tctgccaagg cgtcctcaaa agcaagctga atcgactcga ccgcgatttc
    16021 ggcggtttcg gcgggaaggg cgcggattaa ggctttgaat gcggtggaca ggacgtggtt
    16081 ctgcatacgc agcacgtcgt tcgcctcttc cagatattcc aaacgctgtt cgatgctgcc
    16141 catgatgtat cctcgcgaat ctgttaaaat gcgccattat atcaaaccgc gctttgggcg
    16201 cgggtttgtc tgcttgcaaa gattaagaac cggaagccga aaatggatgc gaataaagag
    16261 ccgaattggg aaaaacgttt ggaaaacctg cgtcagacgc gccgtatgaa ggcagtgccg
    16321 acttacgcgc cgctgtcccg cccctctgaa accggaacgg acgcgcctgt gccgaaactg
    16381 gacgatgaga gccgagcccg tgtgttgaag gcgtatctga aaaaatggca ggaggaacac
    16441 aatgccgccg ttgccgattg cggcgaggaa gtcgaaaccg acccgatgat cgtccttcag
    16501 gaaaactggc tcaacgcgca ggcttccctg caaatgtcgg tttcggaaaa acacatcgaa
    16561 tcgcggcgga gaatccggtt tgataccaaa aaagacagtg tcgaatttga aaccggacag
    16621 attgaaagcg gggaaaacgc cgacgaggca gaatccggca cgaatgagcc ggcggtttcg
    16681 gaaaacgggg aaaacgatgc ggaagaaacg ttgatgcccg tccaactcaa tatcctgaac
    16741 ccgaaagccg tcaaccggcg cgaagtgttc tgcctgtccg aacaggagct gaccgaacgg
    16801 ctgatcaaac gcctgcgccc gcatctgacc gataccgtca acggtatcat ccgtacggcg
    16861 gtgcaaaagc agatggcggt ttttacctat cagctccagc agatgctgca cgagcaggcg
    16921 ggtgcggtag tcgaagacgt gttggaacac gatgtccgca aaatcctcaa cgacatcaag
    16981 tacgagttaa aatacaaacg ctgattgcca tgccgtctga aagccgtttc agacggcatt
    17041 tgcattctca cccgacttga aagcatcata gaacaggcgc aaaatattaa caaattaaac
    17101 aacgggcgca cgccccgttt ttgaacggac aaaaccgagg aatcaccgat gagcaaaaaa
    17161 ctccatccgc aaacgctcgc catacgcggc ggcaaagaac aaaccgaata ccgcgaacac
    17221 aatcaggcat tgttcctgac cagcagcttc atgtgggaca acgcccaaca cgctgccgat
    17281 ttgttttcaa aaaaaatcaa aggcttcact tatacccgta ccgccaaccc gaccacagcc
    17341 gcctttgaaa aacgcatcgc cgctttggaa ggtgcggaac gcgcggtcgc cacttcgaca
    17401 ggtatgtccg cgattcaggc ggcgtttttt accttcctgc aggcgggcga ccacgtggtt
    17461 tccagccgca gcctgttcgg cacgaccgtc ggctttatca ataacatcgt taccaaattc
    17521 ggcatcgaag tgagccacgt ttcgccgact gatataaacg aatggaaagc cgccgtcaaa
    17581 gccaacacca aactgctgtt tttggaaacg ccgtccaacc ccttgggtga agtggccgac
    17641 ttggaagcct tggcggaatt ggcgcacggc atcggcgcgc tcttggtggt ggacaacagc
    17701 ctgctgtcgc ccgtcggctc gcagcctttg aaacacggcg cggatatttc cgtttcctcc
    17761 gcgaccaaag ccatagacgg acacggacgc gtgatgggcg gcgtgttggc gggttcggaa
    17821 gaactgatgg cgcaggtcgc cggttactgc aactcttgcg ggctggcaat gtcgccgttt
    17881 aacgcatggc agttgttgag cggcgtagaa accctgtcgc tgcgtatgga aaaacagttt
    17941 gacaacgccc tgaaaatcgc gcaatggctg caagcgcagc cgcaggttca agccgtgtac
    18001 tacaccggat tgcccgacca tccgcaggca gagcttatcc gcaaacagca aaacggcggc
    18061 ggcatcgtca tcggctttga agtcgccgac caagaagccg cgtggaaagt cgtggacggc
    18121 gtggaactct tttcccgtac cgccaacctc ggcgacgtgc gctcgaccat cacccatccg
    18181 tggacaacca cgcacggcag gatgcagccc gaagaaaaac tcgccgccaa catccgcccc
    18241 ggattggtgc gcctgtcggt cggtttggaa tatgttggcg atttgattga cgatttgaaa
    18301 caggcactcg cgcgctgatt gcgtgtttca gacggcatcg gcggaaaaac accgcaaatg
    18361 ccgtctgaaa cccttatctt atatggcata cacgtcaaca ataggaaaaa aatatggatg
    18421 aagcggtttt tgccgattgg gcgttgaaaa tctgcctgac cggcttgatt atttttttgg
    18481 gttttatcgt ttggaatttg ggcaaggagt ccaaagcagg caaattcggc atcgccattt
    18541 tgtttttggt tttgggcttg ggcgtgttcg gttttgtatt taaagaactg ctaatcaagt
    18601 ttttggtatt gcccaaatag gctgccgcct gtcaaaatgc tgtctgaaat cataaaaagt
    18661 aatctgcacc ctaaaagttt gatttaaccg ccaactgatt aaggtgcgga tttttatgac
    18721 aaaatatagt ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactatttgt
    18781 actgtctgcg gcttcgtcgc cttgtcttga tttaaattta atccactata aaaatcatct
    18841 ttccgcagtc attcccacgg atgcgaaagc ctgtcttttt gaatcccgga taattttaca
    18901 tcgtcattcc cgcgcaggtg ggaatccaga tcattgggta gcggcaatgt tcaaaagtcg
    18961 tctgaaaaat cagaagttct agattcccgt ttttacggga atgacggaat ttcagacggc
    19021 atccgcccgc cccgtcattc ccgcgcaggc gggaatctag tccgttcggt ttcggtcatt
    19081 tacgataaat tcctgttgct tttcatttct ggattcccac ttccgtggga atgacggcgg
    19141 agcggataaa ttctcgcaat ctaaaacctt gtcatttcca taaaaaacag caacccgaaa
    19201 cagcaactta aaaacagcaa cctgaaacct cgtcattccc gcgcaggtgg gaatccagat
    19261 cattgggtgg cggcaatgtt caaaagtcgt ctgaaaaatc agaagttcta gattcccgtt
    19321 ttcacgggaa tgacggaatt tcagacggca tctgcccgcc ccgtcattcc cgcgcaggcg
    19381 ggaatctagt ccgttcggtt tcagtcattt acgataaatt cctgttgctt ttcatttctg
    19441 gattcctact tccgtgggaa tgacggagga gcggataaat tctcgcaatc taaaacctcg
    19501 tcatttccat aaaaaacagc aacccgaaac agcaacttaa aaacagcaac ctgaaacctc
    19561 gtcattcccg cgcaggcggg aatctagtcc gttcggtttc ggtcatttac gataaattcc
    19621 tgttgccttt catttctaga ttcccacttc cgtgggaatg acggcggagc ggataaattc
    19681 tcgcaatcta aaacctcgtc atttccataa aaaacagcaa cccgaaacag caacctgaaa
    19741 ccttgtcatt ctcgcgcagg cgggaatcta gtccgttcgg tttcggtcat ttacgataaa
    19801 ttcctgttgc ttttcatttc tggattccca cttccgtggg aatgacaaac tttaaggtgt
    19861 gatgatttat ccaaaacagt cgaaacgcaa aaaccggttt ctcgtttgca taagaacggc
    19921 aaatttttcg gtgtcttgtt ttatgggcgt tatcccttgt ccgtcccgta gggcaggtaa
    19981 acgtcgaagc gggtgctttt gccggtgtat tggtaggcag gggcttgtcc ggcaaggtgt
    20041 tcgccgaggc gggggcgttt gacgacgacg cgtttttttg ctgtttggcg tgcggtatgc
    20101 aggagtgccg cttcatcttg cgcttcgccg acgagccggt ggaagtaggt catttctttt
    20161 ttaacggcgg cacttttgcg gcgttcggga tacatggggt cgagatagac gatgtcgggt
    20221 ttgccttgtg tttgaacaag ggcgggcatt tgtactgcgg agttgccgaa atgcagggtg
    20281 atgcgcgcgg cggtgtcttg cgtttcggga ttgaggaggg cgcggcggat gccgtctgaa
    20341 agcaggcagg cgacggcggg atgttgctcg aaggcggtaa cggtcagccc gagcgaggcg
    20401 aggacgaagc tgtcgcgccc caatcctgcg gtcgcgtccc aaacggtcgg tttggcggta
    20461 tggttgacgg ctttggcgat gagttcgccc ccgccttttg tgcgccggta ttgtgccgcg
    20521 ccggaggcaa aatcgacgat gacgctgctt ttttccccga caagcctgag gctgacggta
    20581 tcgtgttcgg caaggaggta actgccttgt tcgggcggtt gggaaacggg cacaaggggg
    20641 aatgcccgta tcagggcgcg gacggcttcg gtggcggtgt tgtcaataag gatgtcggtc
    20701 atggtgtttt gtgtccgttc gggcgtgggg cgaacccgct ttcccgtgcc gtttcagacg
    20761 gcattttttc gggtgggcgg agatggtgcg gatgccgttt tgtcgtcagc cgatgttgag
    20821 gcgttccata cggtagcgca tggaacggaa gctgatgccc aagcgtttgg cggcctgcgt
    20881 gcggttgcct tcggtttgtt tgagggtttg ttcgatgatg tcgcgttcga tttggtcgag
    20941 atagtcctgt atctgcatgg tatcggggtc gaacgcgccg caacgcaggg cttcctgtgc
    21001 ctgatcggca ttgccgaagg cggtgatgac ggcaaccggc gtatcgagca ggcggctgtt
    21061 gatgtgttgg acgacttcga gccccgagcc gtccggcata cgcatatcgg tcaggacgag
    21121 cgaatagcgt tggctgtcga gcttgttttt ggcttcggca acgcctgacg cggtatggac
    21181 gcgcaagccc attttcatca gggtcatttc catcaggtcg agaatgtcgg tttcgtcatc
    21241 gacgacgagg acggggtgtt gcagttcagt cattggtttt ttccggtaat gtgagttcga
    21301 aacatttggc ttccggtagg taggtcaaat cgccgaaatt ggcgtgcgcc agttcgcggg
    21361 cgacatacag cccgaggccg gtgccgtttt ccgccgtggt gtaaaagggt tcgaacaggt
    21421 gttcctgcac ttcgggcggt cggcaaagag gatacagacg gtgttttttt gcgcggggcg
    21481 gatggtgacg gaaatcgagc cgggctgttt gcggctgtgc cgccacgcgt tgttggcgag
    21541 gttccacata atttgccgca ggtgggcagg atcgaaatag gcagtcaggt gattgccctg
    21601 catatctagg cggatgcagc cggcggcatc gggatggccg agcaggaact cttgtttgaa
    21661 ttcttcccaa aacggtatca ggccgatggt ttcgcgttcg gttttgttgc gcttgttgag
    21721 cgaggaaata tcttcgagca ttttgtcgat acggcagacg ttgccgtcga tgattttgca
    21781 caatttggcg ttgaacggat ctgccgcccc cgcttccata ttttcgcgca acaggtcgtt
    21841 tgcatggcgg atggcggaca tggggttgcg gatttcgtgg gcgatgttgg cggtcagttg
    21901 tccgagcgcg gcaagtttga cggacagggc ttctgcctga atttcgcttt gcgggcggat
    21961 gtagaggatg agcagcttgt tctgctcttt gttcatcggc acggcgcgga tgcgggcggt
    22021 cagggcgggc gtgtcgatat tgcgttcgaa agtacgcgaa ggggccttgt cccataaaac
    22081 ggtaataggg tcgaacaggg cggtatgctg tccgatttca agcgtgggga gcagatcttt
    22141 tgccttttta ttgaacagta cggtctgata ctcgacgttg atgatgacga ccgcttcctg
    22201 aacgcggttg agcacgattt ggttcaagcc cctgatgcgg cggtaggcgg cgtggttttc
    22261 atgggcaagt ttgccggcac ggtcgatgta tctgaccgac agcgaggcaa tcatggcgac
    22321 gaaataggag ccggtcacga ggacgaaggt gttggtgacg gtttttgcat ccaaaatcag
    22381 cggatacata ttgatattgc tgtctgcaag ggcattcaat atcagcagga tggcggcgta
    22441 gctggcatag agcagggggt aacgcccgta actgagcagg caggagctgc ccacgaaggg
    22501 caggatcagg atgccgaacc ccgagttgat gccgccgaac aggtaggtca gcacgccgat
    22561 catcgtgatg tccgccaccg cgctgaaact cggtattctc aaagcctgcc attgccaatg
    22621 cggatggatg atggaaaaga aaatcagcca gcaggcaatg gcgagccaaa cataaaacgc
    22681 ccacgccgtc cacgaagcgt gcaggttcgc acggctgccg gtttccagcc cgaggatgtg
    22741 cataatcatc agcggaaaaa cgatggcgac gcggatgatg ttgatcagat tgggaatccg
    22801 gtctttgagt ttttcaagtt cgcgggggtt ggaaatcacc ataattatgt gtggaatcgg
    22861 gtttatttgg gggaagaaag ctgtttcagg ctgcccgata tgggtaatag tttgcctttt
    22921 ttgccgcgtt ttgcctcaag cagggagacg gggatgcggt ctttgtgcgc cgcgccgcgc
    22981 ctgccttcgc tttcaatcag gatttccggc tcggaagaaa cggcggtatg cgtcagcgat
    23041 tcgccggcgt ttaatccgat gatttgcagc cctttgcctt tcgccataat tttcaattcg
    23101 ccgatgggga aggcgagggc gcggttttga ctggtggcgg caatgatttt gcagtcgggg
    23161 ttgatgaacg aggaggcata gacggcaatc ggcggcagga cggtttcgcc actgtctgtg
    23221 gtcatcacca ctttgcccgc ttttacgcgt ccgaccatat cagccaactt ggcgatgaag
    23281 ccgtagccgc cgctgcttga taataaataa tgttgttccg gcaatcctgt caacatcgca
    23341 acgggtttcg cgccgttttg caactcgatc aaggatgaaa ccggcacgcc gtcgccgcgt
    23401 ccgccgggaa tttcggcggc atcgagtgta taggttctgc ccaatgaatc gaggatgacg
    23461 acgggtaaaa ccgttctgcc ttcaagggtt tgtttgaggc ggtcgccttc tttgaacgcg
    23521 gtttggctca aatcgagatt gtgtccggca cggctgcgta tccagccttt ttccgacaga
    23581 atcagtgtga tgggttcgtc ggcggtggtt tgtgtcagca cggcgcgtcc ggcctcttcc
    23641 accagcgtgc ggcgtgcatc gccgtattgt ttcatatccg cctgcatctc tttgataatc
    23701 agcttgcgct tttcgttttc atcgcccaaa aggatgttca gacggccttg ttcttcgcgc
    23761 aattcgttca attctttttc gagtttgaaa ccttccaaac gtgccaactg gcgcaggcgg
    23821 atttccaaaa tgtcttcggc ttggatttcg gtcagcccga acgccgccat caaatcggct
    23881 ttcgggtcat ctgattcgcg gatgacttta atcacttcgt cgatgtgcag aaagactttc
    23941 agacggcctt cgaggatgtg cagccgtttt tccacttggt tcaaacggaa tttcagacgg
    24001 cgtgttacgg ttacggtgcg gaaatccagc cattcctgca aaatcgtttt caggtttttc
    24061 tgcgcggggc ggttgtccag ccccatcatc accaagttca tggacacatt gccttccagc
    24121 gaagtttgcg ccatcagcgt gttgatgaag gtatcggtat cgatgcggct ggatttcggt
    24181 tcgaacacca gtcgcacggg atgttcgccg tcggactcgt cgcgcacgcg gtcgattaaa
    24241 tccagcatca gctttttggt attgagctgg tcttggttga gctgtttctt acccgctttc
    24301 ggtttcgggt tggtttgctc ttcgatttcg gcaaggattt tggcggaatt ggcgttcggc
    24361 ggcagctcgg ttacgatgac gcgccactgt ccgcgcgcca atttttcgat ttcataacgc
    24421 gcacgcacgc gcacgctgcc cttgccggtt tcataaatcc ggcgcaattc gtccgccggc
    24481 gtgatgattt gaccgccgcc ggcaaaatcg ggggcaggaa tatattgcat caggtcggcg
    24541 gtttccagcg tcggcttttt caacaacgca atcgccgcct gcgtcacttc gttcaaattg
    24601 tgcggcggaa tctcggtcgc catgcccacc gcaatgcctg acgcgccgtt gagcaacacc
    24661 ataggcaggc gggcgggcag gtgcagcggt tcgtcaaacg cgccgtcgta gttcggcaca
    24721 aaatccaccg tcccctgatt gatttcggac aacagcaatt ccgcaatcgg cgtcagccgc
    24781 gcttcggtgt aacgcatcgc cgccgccccg tcgccgtcgc gcgagccgaa gttgccgatg
    24841 ccgtcgatta agggatagcg caaggtaaaa tcctgcgcca tccgcaccat cgcctcatag
    24901 gcggaactgt cgccgtgcgg gtggtattta cccaaaatct cgccgaccac gcgcgccgat
    24961 ttcaccggct tcgcccccgc cgtcaaaccc atatcgcgca tggcaaacaa aatgcgccgc
    25021 tgcacgggct tctggccgtc tgaaacttca ggcagcgcgc ggcctttgac cacgctcatg
    25081 gcgtattcga gataggcgcg ttcggcgtat cggccgagca tcagcgtatt ggaatcggta
    25141 tgtggggcgt gcgcttgcgt attcattgtg tatgcggaat gtaaagcaaa ggacattatt
    25201 gtaaatcaaa acggcaaacc atagtgggcg cgtgtctgaa tctgaatcaa atacgggaaa
    25261 tgtgaaaata tgttataaat aaggctttcc cactttcaca cattggagac gatatggaac
    25321 tggtatttat ccgccacgga caaagcgaat ggaacgcgaa aaacctgttt acaggctggc
    25381 gcgacgtgaa gctgagcgag caggggcttg ccgaggctgc cgccgccggc aagaaactga
    25441 aagaaaacgg ctatgagttc gacatcgcct tcacatccgt cctgacccgc gcgattaaga
    25501 cctgcaacat cgttttggaa gaatccgacc aactgttcgt accgcaaatc aaaacgtggc
    25561 ggctgaacga acgccactac ggccaactgc aaggcctgga caaaaaacaa accgccgaaa
    25621 aatacggcga cgagcaagtc cgcatctggc ggcgcagcta cgacaccctg ccgccgcttt
    25681 tggacaaaga cgatgagttt tccgcacaca aagaccgccg ctatgcccat ctgcctgccg
    25741 atgtcgtacc cgacggcgaa aacctgaaag taacgctgga gcgcgtattg ccgttttggg
    25801 aagaccaaat cgcccccgcg attttgagcg gcaaacgcgt cttggtggcg gcgcacggca
    25861 actccctgcg cgcgctggca aaacacatcg agggcatttc cgacgaagac attatgggct
    25921 tggaaatccc gaccggtcag ccgttggtgt acaaattgga cgacaatttg aaagtcatcg
    25981 aaaaattcta cctgtaaggg tgtgaaataa aaaatgccgt ctgaatccca gcgtttcaga
    26041 cggcattttt gacaaggcgg caaaacgcat cagctgcgta ccaagaaacg ccccgtcgct
    26101 atccggtgca gcgtaccgac aagcagcagc gccacgcctg ccgatccggc ggtggcaagc
    26161 gtcagcccca agcccgacaa tacgccggaa gccgccagat gcagaccgca tcctgccatt
    26221 gccgcgaaac cgaatgaaaa tccccaaaag ctcatcgtaa aaccaccttc ccaaaaccag
    26281 cgggtcaggc gcagcaagaa caaaagctgc aggcagccgt agccgattaa tgccaacgca
    26341 aaaccgtcga ctttaccgcc gacggcaaaa tacgcgccgc agccgacaaa ggcgggcgca
    26401 agctggatgc cgaccacgcc gcgcgccgcc gtgccgaccg gtgccgccgt gcgcagcctg
    26461 cccaagatgg aggcttccaa gctcagccag gaaaacatac ccgcgccgaa aaacaaagct
    26521 gcataatcat gatgccccaa cgccgccaga gatgacgcgc tgacaaagtt tgtcgcaacc
    26581 gtaggcagat aaataatcgg cgtcgtcgcc tccaaggaat gcaggccgcg ccacagaccg
    26641 gccgcccgat acatcgaaaa agccaactgt ccggcaacgc cgacataaat caggactgcc
    26701 gccgcgcctg cctgataggg cttcagcgcg agtccctcca gcatagcggt aatcggaatc
    26761 gcgctgatga agcagcattg caccaagtcg cgtaaatcag acaaaaaatc gtttcggtac
    26821 gcaaacattt tgatcaggta tgccgccacc agcaagagcc agacgaccga agccgccgca
    26881 agcagcgatt cggcggccaa ggcgggcagc agcccgacag acgcgccgta acgccacgac
    26941 agccccaagg caaacaagcc cagcgcgatg ctgaaatagc tcaaaggaat cggaaatttc
    27001 ttagatgcag acataaacac ccttcagacg gcatgccgtc gtaacgcaca gatatagtga
    27061 attaacaaaa accagtacga cgttgcctcg ccttagctcg aagagaacga ttctctaagg
    27121 tgctgaagca ccaagtgaat cggttccgta ctatccgtac tgtctgcggc tcgccg
//
LOCUS       DAWHRA010000008        26883 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWHRA010000008 DAWHRA010000000
VERSION     DAWHRA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.008"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(380..1177)
                     /locus_tag="WIB30_000267"
     CDS             complement(380..1177)
                     /locus_tag="WIB30_000267"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ3796407.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPAREIPLNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVNGVSTVLARLPLTAP
                     QIESEPESEPASQAASVETAANPSAEQRQIEATAAKIQAAVQDVLKD"
     gene            1332..4181
                     /gene="uvrA"
                     /locus_tag="WIB30_000268"
     CDS             1332..4181
                     /gene="uvrA"
                     /locus_tag="WIB30_000268"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ3796408.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPTVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPDRMLIL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIHGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIAKGSPEQVAKVKGSYTGKYLKVVLR"
     gene            4215..5810
                     /locus_tag="WIB30_000269"
     CDS             4215..5810
                     /locus_tag="WIB30_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ3796409.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(6117..6332)
                     /locus_tag="WIB30_000270"
     CDS             complement(6117..6332)
                     /locus_tag="WIB30_000270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796410.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFLPFWGSQRPLQNSPK
                     SPKFPPRHLGDFGEFCKGLGLSVPVCA"
     gene            complement(6361..7251)
                     /gene="sucD"
                     /locus_tag="WIB30_000271"
     CDS             complement(6361..7251)
                     /gene="sucD"
                     /locus_tag="WIB30_000271"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ3796411.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(7262..8428)
                     /gene="sucC"
                     /locus_tag="WIB30_000272"
     CDS             complement(7262..8428)
                     /gene="sucC"
                     /locus_tag="WIB30_000272"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ3796412.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(8527..8814)
                     /locus_tag="WIB30_000273"
     CDS             complement(8527..8814)
                     /locus_tag="WIB30_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796413.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(8939..10372)
                     /gene="lpdA"
                     /locus_tag="WIB30_000274"
     CDS             complement(8939..10372)
                     /gene="lpdA"
                     /locus_tag="WIB30_000274"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ3796414.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            10569..10682
                     /locus_tag="WIB30_000275"
                     /pseudo
     CDS             10569..10682
                     /locus_tag="WIB30_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10737..11936)
                     /gene="odhB"
                     /locus_tag="WIB30_000276"
     CDS             complement(10737..11936)
                     /gene="odhB"
                     /locus_tag="WIB30_000276"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ3796415.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAAPA
                     EAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAPA
                     VALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKF
                     EKEHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVP
                     ILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINP
                     PQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDP
                     ARLLLDL"
     gene            complement(12036..14864)
                     /locus_tag="WIB30_000277"
     CDS             complement(12036..14864)
                     /locus_tag="WIB30_000277"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ3796416.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGSADEAMLKKQVSVLRLISAYRIQG
                     VGAAKLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(15069..16352)
                     /gene="gltA"
                     /locus_tag="WIB30_000278"
     CDS             complement(15069..16352)
                     /gene="gltA"
                     /locus_tag="WIB30_000278"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ3796417.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSRVAEYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(16464..16712)
                     /locus_tag="WIB30_000279"
     CDS             complement(16464..16712)
                     /locus_tag="WIB30_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ3796418.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(16716..17423)
                     /locus_tag="WIB30_000280"
     CDS             complement(16716..17423)
                     /locus_tag="WIB30_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ3796419.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(17543..19306)
                     /gene="sdhA"
                     /locus_tag="WIB30_000281"
     CDS             complement(17543..19306)
                     /gene="sdhA"
                     /locus_tag="WIB30_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ3796420.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDANTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(19309..19650)
                     /gene="sdhD"
                     /locus_tag="WIB30_000282"
     CDS             complement(19309..19650)
                     /gene="sdhD"
                     /locus_tag="WIB30_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ3796421.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(19644..20021)
                     /gene="sdhC"
                     /locus_tag="WIB30_000283"
     CDS             complement(19644..20021)
                     /gene="sdhC"
                     /locus_tag="WIB30_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ3796422.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(20300..21703)
                     /locus_tag="WIB30_000284"
     CDS             complement(20300..21703)
                     /locus_tag="WIB30_000284"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ3796423.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(21952..22689)
                     /locus_tag="WIB30_000285"
     CDS             complement(21952..22689)
                     /locus_tag="WIB30_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ3796424.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(22968..25244)
                     /gene="metE"
                     /locus_tag="WIB30_000286"
     CDS             complement(22968..25244)
                     /gene="metE"
                     /locus_tag="WIB30_000286"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ3796425.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAAGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHANTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGIIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(25381..26259)
                     /gene="metF"
                     /locus_tag="WIB30_000287"
     CDS             complement(25381..26259)
                     /gene="metF"
                     /locus_tag="WIB30_000287"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ3796426.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            26461..26736
                     /locus_tag="WIB30_000288"
     CDS             26461..26736
                     /locus_tag="WIB30_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ3796427.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            26736..26861
                     /gene="ykgO"
                     /locus_tag="WIB30_000289"
     CDS             26736..26861
                     /gene="ykgO"
                     /locus_tag="WIB30_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ3796428.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
BASE COUNT         6731 a         6803 c         6515 g         6834 t
ORIGIN      
        1 ggaaaggttt ctgtttttta agtgttaggt aactaataag tcgtcattcc cgcgcaggcg
       61 ggaatccaat tcgttcggtt tcagccgttt ccgataaatc gccttagcat ttgggttcta
      121 gattcccact ttcgtgggaa tgacggcgta aaggtttctg ttttttaagt tttaagtaac
      181 taatgagttg tcattcccgc gcagacggga atccaattcg ttcggtttca gccgtttccg
      241 ataaatcgct gtaacgttaa gttcccggat tcccattttc gtgaacataa cggtgtgaac
      301 attagcaaaa attcaaaaaa ccaaacttcc tcattcgtgt gaatgatggt actgatattt
      361 tctattttag ttcgcaaaat taatctttca acacatcttg cacagccgct tgaatcttag
      421 ccgccgttgc ttcaatttgc cgttgttcgg cagatgggtt tgccgctgtt tcaaccgaag
      481 ccgcttgtga agctggctca gattcaggct cggattcgat ttgcggcgca gtcagcggca
      541 aacgcgccaa tacagtgctg acgccgttta ctttatcgcc aatcgccact tgcgcctgcg
      601 catcgacagg cagatacata tccacgcgcg agccaaaacg gataaagcca tagcgttcgc
      661 cgcgagacag tttcgcacct gcttgggtgt agcacaaaat acggcgcgcc accaaaccgg
      721 ccacttgaac aaaagtaatt tcacgacctg aagccgtagt cgccaacacc gcattacgtt
      781 cgttttccgt gctggctttg tccaaatccg cattcacgaa tttgcctttg ttatagacca
      841 ctttcgttac cgtacaatcg gcaggcgatt tttgcgaatg cacgttgaac acgttcataa
      901 aaatactgat tttcaaagca tcgacatcac gatacggatc gcgtgcgcgt tcgaccacca
      961 cgatacggcc gtcaaccggg ctcaacaccg cttcaggatt tagcggaatc tcacgcgcag
     1021 ggtcgcggaa aaactgcaat gcaaatacgg taaacaccca aaacggcaaa gaccaccagc
     1081 cgcaacatat cgacaccagc aggctcaaag ccaaaccgcc gccaataatc ggccagccct
     1141 cacgggcgat aatcgggtgg gggtaaagac ggttcatttg tcatcctttt gttcaagcgg
     1201 gttaaaattg ccgacattat agcggaatct gccgtactcc gctggcaaat gccgtctgaa
     1261 cgcggcttca gacggcatcc gagaaagtgc tacaataccg gttttccttt atttttcagg
     1321 cggcatccat tatgtgcaac catcatcccc aacattcgca cgacaacgac accatccgca
     1381 tccgcggcgc gcgcacgcat aatttgaaaa acatcgattt agacattccg cgccacaaac
     1441 tcgtggtggt aacaggattg tcgggcagcg gcaagtcgtc gctggcgttt gacacgctgt
     1501 atgccgaagg gcaacggcgt tatgtcgaga gcctttccgc ctatgcgcgg cagtttttgc
     1561 agatgatgga caaacccgat gtcgatttaa ttgaaggtct gtcgcctgca atttccatcg
     1621 agcaaaaatc caccagccac aacccacgct ccaccgtcgg tacggtaact gaaatccacg
     1681 actatctgcg tcttttatac gcccgtgtcg gtaccccgta ttgtcccgaa cacaagctgc
     1741 cgctgtcgag ccaaaccgta tcgcagatgg tcgatgccgt attgaaactg ccggaagaca
     1801 cgcgtgtgat gattcttgcc cccaccgtgc gcgagcgtaa gggcgagttt gtcgatttct
     1861 ttgccgactt gcaggcgcag ggttttgcgc gggtgcgcgt ggacggcgag gtctatcagt
     1921 tggacgaagt gccgaagctg gaaaaaaaca tcaagcacaa tatcgacgtg gtcatcgacc
     1981 gcgtgaaagt gaaggcggac atcaagcagc ggctggcgga aagttttgaa accgcgctgc
     2041 gccacggcaa cgagcgtgcg ctggcgatgg agatggacag cggcgaagaa cattggtttt
     2101 ccgcacgttt cgcctgcccc gtgtgttcat acagcctgcc cgaattagag ccgcgcctgt
     2161 tttccttcaa caacccgatg ggttcctgcc cgacttgcga cggcttgggc aacaccaatt
     2221 tcttcgaccc cgaaaaagtg gtcgcccacc ccgaattgtc gctggcttcg ggcgcgattg
     2281 acggttggga caagcgcaac cagttctatt tccaaatgat tcagtcgctg gcgcggcatt
     2341 acggtttcga cgtgcaggct gcttgggaaa cgctacctga aaaagtgaaa aaagtcgtgc
     2401 tgcacggctc aggcaaggaa gtcattgatt tcacctacct atccgaacgc ggcaccacct
     2461 tcaaccgcag ccacgccttc gaaggcatca tccccaatct cgaacgccgc taccgcgaaa
     2521 ccgacagcga aaccgtgcgc gaaaaactgc gcgaatacca aaaccaccgc gcttgcccga
     2581 gctgcggcgg cgcacgtttg cgcaaagaag cccgctacgt ttacgtcggc ggcgagccgt
     2641 tgcacgaagt ttccgcctgg ccgctgacca aaacccaccg attctttgaa acgctggatt
     2701 tggacggcaa caaaaaacaa atcgccgaaa aaatcctcaa agaaatcacc gagcggctcg
     2761 gctttttgat taacgtcggg ctggattacc tgaatctctc ccgctccgcc gaaacccttt
     2821 ccggcggcga agcccagcgc atccgccttg ccagccaaat cggcagcggc ctgaccggcg
     2881 tgatgtacgt tttggacgaa ccctccatcg gcctgcacca gcgcgacaac gaccgcctgc
     2941 tcgccaccct caaacgcctg cgcgatttag gcaacagcgt gattgtggtc gaacacgacg
     3001 aagacgccat ccgcgaagcc gatttcgtcg tcgatatggg cccgggtgcg ggtgagcacg
     3061 gcggcaacgt actgattgcc gatacccccg aaaacgtcgc caaatgcgaa aactccgtta
     3121 ccggacaata cctcagcggc aaaaaatcca ttgccgtgcc gtctgaacgc acgcccgtca
     3181 atcccgaccg aatgctcatc ctcaaaggcg cgcgcggcaa caacctcaaa aacgttaccc
     3241 tcgaactgcc gctcggtttg attacctgca tcaccggcgt atccggcagc ggcaagtcca
     3301 ctctgattaa cgacaccctc gccaaaatca ccgcccgcga actcaaccgc gcccaagaag
     3361 aacccgcccc atacgacgac atccacggtc tcgaacacct cgacaaagtc atcaacgtcg
     3421 accaatcccc catcggccgc accccgcgct ccaaccccgc cacctacacc ggcctgttca
     3481 cccccatccg cgaactcttc gccggtgtac ccctctcgcg cgaacgcggc tacaacgtcg
     3541 gcagattctc cttcaacgtc aaaggcggcc gctgcgaagc ctgccaaggc gacggcgtca
     3601 tcaaagtcga aatgcacttc ctgcccgacg tgtacgtccc ctgcgaagtc tgccacggca
     3661 aacgctacaa ccgcgaaacg ctcgaaatcc aatacaaagg caaaaacatc agccaagtcc
     3721 tcgacatgac cgtcgaagaa gcccgcgaat ttttcgacgc cgtccccacc gtatcgcgca
     3781 aactgcaaac cctgatggac gtaggtctcg gctacatccg cctcggccaa tccgccacca
     3841 ccctctccgg cggcgaagcc cagcgcgtca aactcgcctt ggaactctcc aaacgcgaca
     3901 ccggcagaac gctctacatc ctcgacgaac ccaccaccgg cctacacttc gccgacatcg
     3961 ccctgctgct ggaagtcata ggccgtctga aaggcaaagg caactcgata gtgattatcg
     4021 agcataatct ggatgtgatt aaaacagcgg attggattgt ggacttgggg ccggaaggcg
     4081 gagatggcgg ggggaggatt attgctaaag gtagtcctga acaggtggcg aaagttaagg
     4141 ggagttatac tgggaagtat ttgaaggtgg ttttgaggta agtttaattc aaaattaaat
     4201 aggagattat taagatgaaa ccgttaaaaa cattagaatt tggatttgtg gatgctgcaa
     4261 actacagaag aagagaaaat aaagatttat ttaaccgaat atttgtaaaa ggagaatatt
     4321 tggatgaatt atgtgaacca aatatttcgt ttttaatcgg agaaaaggga actggaaaga
     4381 cagcatatgc tgtttattta actaataact tctataaaaa catacatgcc actactaagt
     4441 ttgttcgtga aaccgattat tcaaaattta ttcagctaaa gaaagcaaga cacttaactg
     4501 tttcagattt tacaagtatt tggaaagtca ttttatatct gttgatatca aatcaaatca
     4561 aatgtaaaga aaacggaata ttatcttcaa tatttaataa atttaaagcc ttagatgagg
     4621 ctataaatga atattattat ggcgcttttg atccggaaat tgtacaagca ataactttaa
     4681 tagaaaattc aaaagaagct gcggaaatga tttttggaaa atttgttaaa ctaggtgaag
     4741 aggaatccca acaaataact tttacagaaa gtaaattcca agcaaattta ggttttattg
     4801 aaagaaaatt taaagatgct ttatctcagt taaagctaaa agataatcat attttgttta
     4861 ttgatgggat agatattaga ccatcacaga ttccatttga tgaatatcat gagtgtgtaa
     4921 aaggtcttgc taacgccata tggatgttaa ataatgatat cttcccttcc attaaagata
     4981 gtaagggaag gatgagagtt gtgttattga ttagacctga tatctttgat tcattaggtt
     5041 tacaaaatca aaataccaaa cttcaagata attcagtatt tttagactgg aggacggatt
     5101 ataaatctta tagaagttca aagatttttg gcgtttttga tcatcttttg agaacccagc
     5161 aagaaaaaca agatagttta gaaaaaggca actcatggga ttattatttt ccatggaatg
     5221 ctcctaattt acatgatgag tataaaaatt taacttcatt tattagcttc ctaagaaaat
     5281 cgtattatcg acctcgcgat attcttcaga tgcttacttt gctacaaaaa aataagaaaa
     5341 gtaaggaaga ttatgtcgta gcagaagatt ttgataatac ttcttttcaa agagaatact
     5401 cgatatattt acttggtgaa atcaaagatc atcttttgtt ttattatagt caaagtgatt
     5461 atcaaaattt cctgaaattt tttgaatttt taaacgggaa agatagattt aaatatagtg
     5521 attttttaaa agcatttgaa cgtttgaaaa agcacttaca aacaacatca gtggaaatac
     5581 ctaaatttat gagtactgct aatgagtttt tgcaattttt atttgacttg aatgttattg
     5641 cttatttaga taacccagaa gatgaaacga aaccatatat ccattggtgc tttaaagata
     5701 gaaattatgc aaatatttct cctaaaataa aaactgaaac tgaatattta atattttcag
     5761 gattatcaaa agcccttgat gttggtactc catttaagaa caaacagtaa cttgtatccc
     5821 aaccaaccgt gtgcgctacg cacacggttg gtagctgacg tgttgcagtt tcaacagaga
     5881 aagccgtagc ctgcataccc aaccgaccgc gtgcgtggct gtgccacacg ccctacctgt
     5941 gaattacacg gaacttcaaa gccaatacga gagctttgca aaattccttt ccctcccgac
     6001 agccgaaacc caaacacagg ttttcggctg ttttcgtttc aaatatccac taattttacc
     6061 ccaatacccc cttaatcctc ccaggatacc cgataatcag gcatccgggc cgccttttag
     6121 gcgcaaacag gcacacttag cccgagacct ttgcaaaatt ccccaaaatc ccctaaatgt
     6181 cttggtggga atttagggga ttttggggaa ttttgcaaag gtctctgact gccccaaaag
     6241 ggcaaaaata atgtgggtat gttgaggctg cctctttttg aaatgacaaa caaaatgccg
     6301 tctgaaaccc gatttccggt ttcagacggc atttgatgtt caatcagttg tcaaacctga
     6361 ttatgccaaa ccttttgctt tcaacacttc cagcatggta gtgcccaact cggcagggct
     6421 gcgggtgtaa gcgataccgg ctttttcgaa agcggcgaat ttttcttccg cagtaccttt
     6481 gccgccggag ataatcgcac cggcgtgacc catgcgtttg cctttaggtg cggtaacacc
     6541 ggcgatatag ccgacaacag gtttgcttac gttggattgg atgtattcgg ctgcttcttc
     6601 ttccgcagta ccgccaattt caccgatcat gatgatggcg tcggtatccg ggtcttcttg
     6661 gaaaagtttc agtgcgtcga tttggttcat acccggaatc gggtcgccgc cgataccgat
     6721 acaggttgat tgacccaagc ccagtttggt ggtttgtgcc acggcttcgt aagtcaatgt
     6781 accggaacgg gaaatgatgc cgatgcggcc gggagtatgg atgtggcccg gcataatgcc
     6841 gattttgcac tcgcccggag taatcacgcc cgggcagtta gggccgacca aacgtgttcc
     6901 gttaccgttg gtttccaagt agcgtttggc tttgagcatg tccaaagtcg gcacgccttc
     6961 ggtaatcaca acgaccaagc ctacgcctga atcaactgct tcaacgatag aatccaacac
     7021 aaacggagca ggaacgtaaa tcacggatgc atccgcgccg gtttctttaa cggcttcttt
     7081 catggtgttg aacacgggca ggttcaggtg ggtttgaccg cctttgcccg gggtaacgcc
     7141 gccgacaact ttagtgccgt aagccagagc ttgttcggag tggaaagtac cgtttttacc
     7201 ggtgaaacct tgaaccaata ctttagtgtc tttattaatc aatacgctca ttcttttctc
     7261 cttaggcgtt tacggctgca acaatttttt cggctgcgtc attcaggccg tctgcagaag
     7321 tcagtttcag acctgattcg ttcaggattt tcgcgccgag ttcggcgttg ttgccttcca
     7381 aacgaacaac gacaggaacg ttgacgttga tttctttaac ggctgccacg atggcttccg
     7441 caatcatgtc gcaacgtacg ataccgccga agatgttgat caatacgcct tgaacggatt
     7501 tgtcttccag aatcagtttg aacgcttcaa ccacgcggtc tttggttgcg ccgccgccaa
     7561 cgtccaagaa gttggcaggt tggccgcctt tcagtttgat gatgtccata gtggccatcg
     7621 ccaaaccggc accgttcacc atacagccga tgttgccttc cagggcaaca tagttcaggt
     7681 caaattcaga agctttcaac tcgcgttcgt tttcttgaga tttgtcgcgc aattcggcga
     7741 ttttcggcag gcggtagagc gcgttgctgt cgatgccgat tttgccgtcc acgcaggcga
     7801 gcgcgccgtt ttcgcgaact gccagcgggt tgacttcaaa cagggcgaag tcattttcga
     7861 caaacgcttt gtacgcaccg gtcatcagtt tgacgaactc gttgatttgt ttgtctttca
     7921 agcccagttg gaacgcaact tcgcgagctt ggcaaggttg caggccgacc agcggatcaa
     7981 cggttacttt gaagattttt tcaggagttt cggcagcaac tttttcgatt tccacgccgc
     8041 cttcggtaga ggccatgaat gtaatgcggc gggtagaacg gtcaaccact gcgcccaagt
     8101 acagctcggt ttgaaccgga tacatgtctt cgcaaaccaa aacactgttg acaggttggc
     8161 cgttggcatc ggtttggtaa gttaccaagt tggtgccaat caggctttca gccacttctt
     8221 tagcttcttc gcggctttta acgactttta cgccgcccgc tttaccgcgg ccgccggcgt
     8281 gtacttgtgc tttgacaaca gcgaatttgc cgcccaattt gtcgtaagct gcagcggctt
     8341 cttcgccgtt gtgtgccaaa ataccgcctt gtacgggcaa accgtagcta gccagcagtt
     8401 ctttagcctg atactcgtgt aaattcatgg atttctcctt tagattagtg gaatgccccg
     8461 agtctgcctc caaatggcaa gcccgtgcgt ttcagactac ctttcggcgg tcggaaaatc
     8521 gtcgtttcac ttgctgaaag gcaaccacat ccgcgccagc agatacatgg ctgcaccgcc
     8581 tcccccgaga ataaaaaaga cgatgccttt tttacgcgaa ttcggacaga gcagataaac
     8641 ggcaaggctg aaactgataa acaacaatgc cacgcgccaa accaattctt tatctttgaa
     8701 acggttgagc gtataagttt cggtcatcat cacatacatc tgccacaatg cggccataca
     8761 catgctgatg atgcccgccg gtataaagaa aataccgact atttctttgt tcataatgaa
     8821 tgtaaatgct gtttgtttgt ctgacggaat tataaatcag aaaacggagc tctcggaaca
     8881 ttcaatttgt cgaaggcctt aaggccgtct gaaaaatttc agacggcctt aatgtctatc
     8941 aaccgtgcaa agcgcgtttg tcggccgcca atgcagcttc gtgaaccact tcggacaagg
     9001 ttgggtgggc atggataatg cgggcgatgt cttcgctgct ggcgaagaat tcgagcgcag
     9061 tcacgccttc ggtaaccaat tcgctgacaa ccggaccaat catgtgtacg cccaagatgc
     9121 ggtcggtttt ggcatctgcc aacactttaa ccgtaccttt ggctttgccc attgccaatg
     9181 cgcgaccatt cgcaccaaaa cctgaagtac cttttttgta ctccacgcct tcggctttga
     9241 gctgctcttc ggttttaccc acccaagcga tttcaggatc ggtgtaaatc acgaacggta
     9301 cgttgttgaa gtcgatatgc ggtttttgac cggcaatgcg ttcggcaacg gcaacgcctt
     9361 cgtcgctggc tttgtgtgcc aacatcgggc cgcgaaccac gtcgccgatt gcccatacgt
     9421 taggcaggtt ggtacggcat tcgccatcta ctttgataaa gccgcgctcg tctttttcca
     9481 agcctacggc ttccgcgttc aggcctttgg tgtttggaat acggccgatg gcaacgatca
     9541 gtttgtcgaa tacttcggtt ttggcttcgc cagcagcagt ttcgtaagca acggaaacac
     9601 ctttgccttc agacttgatg tcgccgattt tcacgcccaa ttcgatgctc agaccttgct
     9661 ctttggtgaa gtatttgaag gcttctttgg cgatttgttg gtcggcggca gccaggaagg
     9721 tcggcgcggc ttcaagaatg gtaacttccg cacccacgcg gttccatacg gaacccattt
     9781 ccaaaccaat cacaccggaa ccgatcacgc cgagtttggc aggtacttcg gtcaggttca
     9841 atgcgccttc gttgtccaat acgttcacat tgtcgatagc gacttgtggc agcggacgcg
     9901 gtacggaacc ggtcgctacg atgacgtgtt tggcttcgat aacggttttc tctcctttgt
     9961 tatcgacttc gatttggtaa gcatcgccat ttttaccggc aaaggaagcc gtaccgaaca
    10021 gactggttac tttatttttt tggaacagga atttcacgcc gccggtcagt ttggtcacga
    10081 tggcatcttt gcgctcaatc attttggccg cgtcgaattt tacgtcaccg acagtgatac
    10141 cgtgttcggc aaaatcgtgt tgcgcagcgt ggaaatgttc gctggattgc aacagggctt
    10201 tagaagggat acaacctacg ttcaagcaag taccgcccaa tgcaggggca ttgcctgctt
    10261 tgttaacgcc tgcatcgaca caggcagttt tgaaacccag ttgtgcggca cggatggcgg
    10321 caacgtatcc gcccgggcct gcaccaatca ctactacatc atattgagac atcgtgtcat
    10381 cctttgcttt tagcagtatc tcaaaataaa aacgatactt ttcgtttctt ttttattttg
    10441 aatgactgcg ttttgatggt tttaagaaaa agccgtctga aagtccatat ctgccgattc
    10501 attcgaatca aatgtttcag acgacctcaa tcgttcgttg agcctattgt agcacgatac
    10561 tacattacgc tacatctaaa tatagtggat taaatttaaa tcaggacaag gcgacgaagc
    10621 cgcaggcagt acagatagta cggcaaggcg aggcaacgcc gtactggttt aaatttaatc
    10681 cactataaaa attcagacgg ccttttcatt aacaaataaa aggccgtctg aaacgattac
    10741 agatccaaca acaggcgggc cgggtcttcc aacgcgtctt taatggctac caaggtcaat
    10801 acagcttcgc gaccgtcgat gatacggtgg tcgtaagaca gagccagata catcattgga
    10861 cggacaacaa cttgaccgtt ttcaaccaca gcgcgctctt tagtggcatg catacccaaa
    10921 atcgcagatt gaggcgggtt gatgatcggg gtagacatca tagaaccaaa agtaccgccg
    10981 ttggtaatac tgaatgtacc gccggtcaga tcttcgatag cgattttgcc gtctttggct
    11041 tttttcgcgt aatcaacaat tgcttgttcg atgtcggcaa tgctcatttg gtcggcatcg
    11101 cgcaggattg gcacaaccaa accgcgtggg ctgccaatcg cgataccgat gtcgaagtag
    11161 ccgtggtaca cgatgtcttt gccgtcaaca gaagcattca caaccgggta ttttttcagg
    11221 gcggcgacag cggctttaac gaagaaggac ataaagccca atttcacgcc gtgttctttc
    11281 tcgaattttt ctttgtactt cgcacgcaag tccatgattg gtttcatgtt gacttcgttg
    11341 aatgtagtca gaatggcgtt ttcttgttga gaagccaaga ggcgttctgc aacacgggca
    11401 cgcaggcggc tcattggtac gcgttcttca ggacgtgcgc cggcaggaag tgcaacggca
    11461 ggggcaggtt tggctgcggc attttgtacg tcttctttca atacgcgacc gtcacggccg
    11521 gaaccttgca atgcgttcac gtcaacaccg ctctctgcag ccagtttggc ggcggcaggc
    11581 atagcggcgt tgttttgtgc agcagcagga gcggcggccg gggcagcttc ggcaggagcg
    11641 gcggcaggtg cttcagcagc ggcagtagca gcggcaggtg cttcagcagc ggcagtagca
    11701 gcggtatcga tacgtgccaa aacttggtcg gcaacaacgg tttcaccgtc ttgcgctacg
    11761 atttcaacca atacgccggc ttgtggagaa ggtacttcca aaaccacttt gtccgtttcg
    11821 atatcgatca ggatttcgtc acgggcaact gcttcgccaa cttttttctt ccattccaag
    11881 agcgtgcctt cagatacgct ttcagacagc ataggtactt ttacatcaat aatcattttg
    11941 tgtctccaat ggccctttca gacggcctgt tgtgtgtttt tcattctgca tatgggtgtt
    12001 ccgcctgagc agcctcagac ggaatgctac ttggcttaca atgccaaagc gtcttcaacc
    12061 aattgtttca attgagcaat gtgtttgctt gagtagccca ctgcaggcga tgcgctgctt
    12121 ggacgaccag cataagacag tttttgctct tcgctgataa cgtcttcgat gcggtggcgg
    12181 atttggtaga acgcgccttg gtttttcggc tcttcttgcg cccaaaccac agattttgcg
    12241 ttcggatatt tcgccagttc ggctttaacc tcatcgtatg ggaacggata cagctgctca
    12301 acgcggacaa tcgcaacatc atcttccagt ttacgttcgg ctcgaccggc ttccaagtca
    12361 tagtaaacct gaccggcaca caataccacg cgtttcacgc tgtcgttgct tgcgcgttcg
    12421 gcggtatcgc cgataaccgg acggaaagtc gaaccttcgg tgaagttttc cagcgggctc
    12481 attgcccctt tgaatcgcaa caggcgtttg gacatgaaaa ttaccagcgg tttgcggtat
    12541 gaacctaaga cttgacgttg caagaggtgg aacatttgcg acgcttcaga tggcatgatg
    12601 acttgcatat tgttctcaga acacagttgc aaccaacgtt ctacgcgtgc agaagagtgc
    12661 tcagggcctt gaccgtcgta accgtgcggc aggatggtgg tcagaccgca caagcgaccc
    12721 catttggttt cgcctgaaga caggaattga tcaatcgtca cttgcgcgcc gttggcgaaa
    12781 tcgccgaatt gcgcttccca aatggtcagc ttgtcaggtg cggagcaggc aaagccgtac
    12841 tcgaacgcca tcacggcctc ttcgttcaaa atggagtcga taaccaggaa ctcgcccatt
    12901 ccttcgccca tatggcgcag aggaacataa gtaccgtcgt cccatttttc gcgtttttga
    12961 tcgtgcaata cggcgtggcg gtgcgagaac gtaccgcggc cggagtcctc accggagata
    13021 cgcacgccat gacctttggt tacgaggctg gcgtatgcca aggtttcggc cataccccaa
    13081 tctatggcct gtttgccgga tgccatggct ttacgcgctt caatcacacg ttttgcagtc
    13141 ggatgcaggg caaagccttc cggtacggcg gtaaacttct cagtgagacg ctcaatatct
    13201 gcggcaggca aaccggtttc gatgtgttcg cgccaatctt tgccttggta tttgctccag
    13261 tcaatttgtg tgcgttggaa gttgctcaac gttgtttgct cgacgtgttc gcctttatcc
    13321 aaagcatcac ggtaggcttg gatgtaaccg tcagcctcgg cttgggttac cacgccttcg
    13381 gcaatcagtt gctcggtgta caaagcacgc gcacccgggt gttgcgatac ttttttgtac
    13441 atcatcggtt gggtcaaggt cggatcgtcg ccctcgttgt ggccccattt acggtagcag
    13501 acaacgtcaa tcacgatgtc tttatggaat tttttgcggt aatccaaagc ggcttggata
    13561 gcaaagcaaa cacgctcagg atcatcgccg ttcacatgga taaccggggc ggaaaccatt
    13621 tttgcgatat cggtacagtg tacggttgaa cgggtatcgc ggatatcgga agtggtaaag
    13681 ccgatttggt tgttgatgac gatatgaacc gtaccgccgg tggtataacc gcgtgtttta
    13741 gacaggttga atgttgcttg gttgacaccc agaccgataa atgcggagtc gccgtgaatc
    13801 aataccggca agactttgtc gcggccgttt tcgcccaaac gtttttgttt ggcgcgcgca
    13861 gaaccttcca ccaccgggtt gacgatttcc aagtgtgacg ggttgaacgc caaagaaaca
    13921 tgcatcgggc cgtgcggggt agcaatatcg gagctgaagc ccatatggta tttcacgtcg
    13981 ccgctgggca gtttgatttc ggcacgacct tcaaattcgg caaacaaatc gccgggtttt
    14041 ttgcccaaaa tgttcaccaa aacattcaga cggccacggt gcgccatacc gatgataact
    14101 tcttccacac cgtctttacc ggcgttttga atcaggtagt tcaaaccggc aatcgcgctt
    14161 tcgccacctt cgacaccgaa acgtttctga ccgacgtatt tggtatgcag ataacgttcc
    14221 aaagtttcgg cagcggtcat ttctttcaga atacggcgtt tttgatcggc attgtagtgc
    14281 ggcgtggaca atacgctttc aaaataattg cgtacccaac ggcgctcttc ggtattggga
    14341 atatagatat attccaatgc gatgtgaccg cagtaggttt gtttgaggtt gctgatgatt
    14401 tgggataaag gcagtttgcc ccgattggca aaatcgccct cgcccatatt gaattggagc
    14461 gccatatcgg catctgacag accgtggaat ttcggatcga gggcttcaat atcgcgcggc
    14521 gggatacgtt tgagcggatc aagtttggct gcacccacgc cttggatacg ataagcagaa
    14581 atcagccgta aaacgctgac ttgctttttc agcattgcct catccgcact gcccgcaacg
    14641 gcagatgcaa ttttcttttt cgccaaagta acaaatgatt cgcgaatcgg tgtgtgtgcg
    14701 acatcgacag caaccgtccc cggctgtttg ctcaaatcgg tgaaatactg cttccatttt
    14761 tcatcaaccg catcggggtt ttccaaaaaa gcctcgtaca attcctcaat gtaaggtgcg
    14821 tttgaaccga acaggtaaga gaaattgagt ttttcgtcca tcatggcgtg cgctcttttc
    14881 tttaaaatat aaacaaaggg tagaagtccg cgtcctgacg gctaaactga tgccgtctga
    14941 aaagacattt tattctaccc tattttaaac cggattgctt gtcttgattt catccgacta
    15001 caatcagacg gattcgggaa acaatccggt tctctgatat tgcctgtttg acaatatatt
    15061 aatgtatgtt acctctcgcc tggcggcaca taatcgcggc gttccgaacc ggtataaagc
    15121 tggcgcgggc ggccgatttt cagcgaagga tcgctaatca tctcgtgcca gtgcgaaatc
    15181 cagcccacgc tgcgcgacag ggcgaagatg acggtaaaca tttcggtcgg gatgcccagc
    15241 gcggacagga cgatgccgga atagaaatcg acattggggt acagtttgcg ttcgataaag
    15301 aacgggtctt tcagcgcaat ctgttccaat tccatcgcca gcttgaattt cggactgtct
    15361 tccaagccca attccttcaa aacttcatag caggtttcgc gcataatgct ggcgcgcgga
    15421 tccatattgc ggtacacgcg gtgtccgaag cccatcagac ggtatttgcg ttgtttcacg
    15481 ccttccatat attcggcaac acgggacaca tctccgattt catccaacat tttcaacacg
    15541 gcttcattag caccgccatg ggacgcaccc cacaggcagg cgataccggc agcgatacag
    15601 gcaaacgggt tcgcacccga agagcctgcc agacggacgg ttgaagttga ggcgttttgc
    15661 tcgtggtcgg catgcaaaat aaagatgcgg tcgagtgcgc gtgccaaaac gggattgggt
    15721 ttgtagtctt cacacggcgt ggcgaacatc atatgaagga agttttcgga ataagaaaga
    15781 ttattcttcg gataattgaa cggcagaccg tttgaatagc ggtagcacat tgccgcaatg
    15841 gtcgggattt tggaaatcag gcggtaaatc gcgattttgc ggtgttcggg attgctaatg
    15901 tccaagctgt cttggtagaa cgcagacagc gcgccgacca cgccgaccat catcgccatc
    15961 ggatgcgcgt cgcggcggaa cccccggaag aaccaagtca gctgttcatg caccatcgtg
    16021 tggcggcgga cggtattgtc aaattctgcc ttttgctcgg gagtcggcag ttcgccgtaa
    16081 atcaacaggt agcagacttc caaataatcg gacttttcgg ccagctgctc gatggggtat
    16141 ccgcgataat aaagcaagcc ttgatcgccg tcgatgtaag taattttaga ctcacagctt
    16201 gcggttgaaa caaatccggg gtcgaaggaa aacaaacctg tattttttgt cagcccccga
    16261 atgtcaacca catcgtgccc gatgctggct tccaataccg gcagctccaa acctgcctga
    16321 cccggtacgt tgagtttgat tgatttggac atattattgc tcctttgcaa gttataaccc
    16381 acgttactgc atccgcagca cgttctttgc cttcagacgg cttttgcatt ttgaaatctg
    16441 cctgcaggca gatttcagat gcctcatgcc cgtctgattt tttcaagcat cgggataagg
    16501 tgccctttgt ccgtttccga atgcccgtta atcaaggcaa gcaattcttg atcttgaaat
    16561 tcaaggattt cggaaaactc ggacagctct ttatcgctca aatgctcgaa ttctttttcc
    16621 ataaacctgc cgaagattaa atctaactcc aacaatcccc ggcgggtttg aaaacggatt
    16681 ttccgtttgg caatatcgtc aaaaaccatc atttcttaaa cggcccgttt caacataatc
    16741 tctttaatct taccgatggc tcgggtcgga ttcaagtgtt tgggacatac gtctacgcag
    16801 ttcataatgg tgtggcaacg gaacaaacgg tatgggtcgt tcagattatc caaacgctca
    16861 ttagtgatgg tatctcggct gtccgcaatg aagcggtaag cattcagcaa accggacgga
    16921 ccaacgaatt tatcagggtt ccaccaaaat gacgggcagg cagtcgaaca gcaggcgcac
    16981 aaaatacact cgtacaaacc gtccaactct ttacgctctt cctgagtttg cagacgctct
    17041 ttgtccgcat caatcggatt atcgttgaca acataaggtt tgacggaatg gtattgtttg
    17101 aagaactggg tcatatccac aatcaggtcg cggataacag gcagacctgg cagaggacga
    17161 attttaaccg gctgtttcaa gccacgcaga tcggtcaaac acgccaagcc gtttttgccg
    17221 ttgatgttca taccgtcaga accgcaaatc ccttcgcggc aggagcggcg gaaagacaag
    17281 gtatcgtctt gtgctttcag gcgcactaaa gcgtccaaaa gtttcacgtc ggtcggttcc
    17341 aattccaact cgtaacgctg catataaggc ttggcatcaa catccgggtt gtaacggtaa
    17401 atttcaaaac tcattttttc catgagaagt gttccttttc tcatcaactg cccaatgatt
    17461 ttgtattata aattcaagca gttcaatgtg ggtatggtta gatttcagac gacctttgag
    17521 gcgaagaccg tctgaaaacg catcaataaa cgcgcttggc cggtttgatg tattccacgc
    17581 tcaaaggctt ggtgtgcacc ggtttgtagg acaaggtatt ggcatctgaa tggtacagcg
    17641 tatgtttcat ccagttttca tcatcgcgct caggatggtc gtctgaagcg tgcgcaccgc
    17701 gtgattcttt acgtgcttcg gcagacacca aagtcgcttt cgccacttca attaggttat
    17761 ccaattccaa agcctcgata cgcgcggtat tccacacttt gctcttgtct ttgatttcgg
    17821 tacgtttcac acgctcggca atcgccatga cttctcgaac gcctttgctc agaatctcat
    17881 cagtacggaa cacgccggcg tgcaattgta cggagcgttg cagttcgcgg cgcaatgcat
    17941 caacgttttc accatcagtt tgattgtcca aacgctcgat acgttggcgg gtcagctcgc
    18001 ccgcatccgc aggcaaaggt ttccagtcgc tttgctcttt gatgaattta atcatgctgt
    18061 cgccggcagc tttaccgaat accactaagt ccagcaggga gttcgtaccc aagcggttcg
    18121 caccgtgtac ggaagcacag gcgcactcac ctgccgcata cagacctttt acaggcactt
    18181 cgtattcgtc gccttgagga acgacaactt cgccatggta gttggtcgga ataccgccca
    18241 tcatatagtg ggtagtcggc acaacgggaa tcgggtcttt aatcggatcg ataccagcga
    18301 actgaatgga aatctcacgg atgcccggca gtttttccat aattttttct gcgccgatat
    18361 ggtcgatttt cagtaagaca tggtctttgt ttttaccgca gccgcgacct tcgtagattt
    18421 ccatcgccat cgcgcgggaa acaacgtcgc gagaagccaa gtcttttacg gtcggcgcat
    18481 agcgttccat aaagcgttcg ccgtcggcat tcaacagaat accgccctcg ccgcgtacgc
    18541 cttcggtaat caacacgccc gcacctgcca cgccggtcgg gtggaattgc cagaattcca
    18601 tatcttccaa cggaataccc gcacgcgcgc aaatgcccaa accatcgccg gtattcatat
    18661 aggcattggt agaagaagca taaatacgac cgccaccgcc ggtagcaaac atcacagctt
    18721 tagcgtggaa aatataaact tcgccggttt ccatttccat ggcggttacg ccgacgacat
    18781 cgccgttttc atcacgaatc aaatcttgtg ccgtccattc cacaaagaat tgcgtattgg
    18841 cacggacgtt ttgttggtac aaagtatgca gcatcgcatg acctgtacgg tcggcaaccg
    18901 cacaggcgcg ttctaccgcg cgtttaccgt gttcggcagt atggccgccg aaaggacgct
    18961 gataaatttt accgctttcc acacggtcaa aaggcatacc catgtgttcc aactcaatta
    19021 cggcttcagg cgcggcgcgg cacataaact caatcgcatc ttggtcgccc aaccagtcgg
    19081 aacctttcac ggtatcgtac atgtgccagt cccaacggtc ttcctgcaca ttacccagag
    19141 aggcggaaat accgccctgc gccgctacgg tatgcgaacg ggtcgggaac actttagaca
    19201 aaacggcaca attcagaccg gatttggata attggagggc tgcgcgtaaa cctgcaccac
    19261 caccgccgac aatcacggca tcaaacttgc gaacaggaaa acccatactt acccccaaat
    19321 tactttaatt gaatacacca agcagccgac cagccagacg atggtggcaa cctgcaaaaa
    19381 caaacgcacg ccgaagggtt tgatatagtc catccacaaa tcgcggatac ccacccaagc
    19441 gtgcaagaat acggcgatga agctcacttg ggtaaatact tttacccaag tttgactaaa
    19501 aaatgcctgc catgccgaat attctttagg cagggaaaat agaaccacta aaagtgcaac
    19561 ggtataaatc aacataataa ccgcagtcgc acgttgcatc gcccaatcgc gcaaaccgta
    19621 atgggcaccg gtcaatttac gttctaccat aacaacgctc ccaaaacgac agtcaaaacc
    19681 aatgcagaag caaatacggc tttagcggta ttgcgcgcag tattcagctc aaggcctttg
    19741 tgcgcatcca aaaataaaaa gcggataccg gcgagagaat ggtgcagata agcccacaat
    19801 acaccgatta aaaccagctt gaccaaagga tgggaaacaa tggcacggta agtttcaaat
    19861 gcagactctt gactcagggt accggacagg aaatacagca ggaaaggcag cataataaac
    19921 agcccgaccc cgctgatgcg gtgaaggatg gaaactatcc cgggtatcgg cagacggatg
    19981 ttcggcaaat ccagataaac aggacgcggt ttggcagaca tagtcaattc ctctgtagtt
    20041 atatatcgac tcaaaaaccg taaacccatt gctacactgg gttacataaa cgttaattta
    20101 cctgttttat cccattttga acagtgtatt ttaaaaacaa actgatagat tttgacaaac
    20161 acgcgtggaa acagcagatt aaaagaaaga tttttagttt tgccgccccc ttcccgacac
    20221 ggtatcagca tccgacccca tccccaaaaa caaaatgccg tctgaaaaaa tttcagacgg
    20281 cattgttttg ccatatcggt caggctttca atttcgcatc ggcatcgcgc aacgcggtac
    20341 gcagaccttc ctcgataacg ggatggtaga acggcatatc cagcatttgc ggaacggtca
    20401 tcttcatttg atgcgcccat gccagcaagt gcgccaaatg ttcggcggca gggcctacga
    20461 tttccgcgcc gataaagcgg ccggtggctt tttcggcata caggcgcata tggcctttgt
    20521 tcaccagcat cacgcggctg cggccttggt ttttgaacga tacttcgccg atgacaaatt
    20581 cgtcggcttg gtattgcgcg gcaacctgcg cgtatttcag accgacaaag ccgatttgcg
    20641 gactggtaaa caccacgccg atggtgctgc gccgcaaacc gccgccgata ttcgggtagc
    20701 ggcccgcgtt atcgccggca atcttgcctt ggtcggcagc ttcatgcagc agaggcagtt
    20761 ggttggacgc gtcgcccgca ataaagatat gcggaatgct ggtctgcatg gtcagtgggt
    20821 cggcaacggg tacgccgcgc gcgtcttttt cgatattgat gttttccaaa ccgatattgt
    20881 caacgttcgg acggcggccc acggctgcca gcatatattc ggcaacaaat acgccttttt
    20941 cgccgtcctg ctcccaatgg acttctacat tgccgtctgc atctagtttg acctcggttt
    21001 tagcatccag atgcagtttc aattcttcgc cgaacacggc tttcgcctcg tctgaaacga
    21061 cggggtcgga aatgccgccg atgattccgc ccaaaccgaa aatttcaact ttcacgccca
    21121 aacggtgcaa tgcctgaccc agttccaaac cgataacacc cggcccgaac acggcaacgc
    21181 gcttaggcag cgtatcccat gagaaaacgt catcgttgat aatcaaacga ttgcccaaag
    21241 actgccattg cggcaggatg acgggacgcg aaccggtagc aatcacgaaa ctttttgccg
    21301 taatttgagt atgctcgtcg atttggacgg tatgctcgtc gataaattta gccgaaccca
    21361 tgatgcgctt gtcggcaggc cactcttcca catcggcaac gacaaagccg acaaaacggt
    21421 cacgctcgga tttaacgcgc tgcatgacct cttcaccgtt gacgacgatg ctgtctttgt
    21481 ccaaatgcac gccgaacggg tcggtatgca atgcgtggtg gcgcgcttct gcggcggcaa
    21541 tcaagagttt ggaaggcata caacccacgc gcgcgcaggt tgtgccgaac acattgtttt
    21601 caatcaggta aacattatcc gaatgtaaac gggcattgcg aaacgcaccc ataccggcag
    21661 taccgccgcc gattacgacg acatccgctt gaattttttt cataatcttt gtccttccgt
    21721 tttcgtctga aacattgccg acacagaaca tatcggtttc agacggcatt taaatatttt
    21781 cccaatcaaa aacatcattc agataaataa aaacaaaatt tcgattgagt gcaacaatat
    21841 ccaaattctc ttataaatat agagttattc ggatcggcag tgtagaaccg cccgaataac
    21901 tctatccggt atatcagaca gtttaatcaa accgccttaa gcaaacagct atcagttttt
    21961 agccaagtaa gcttccaaat cttcgctgcc gccgatgtat ttgccgccga tgaagacttg
    22021 aggggcagtc atcttgccgg taatggcgcg aacggaagta acggttgcat ctttgcccaa
    22081 tacgatttct tcgtaagaca aacctttgtc ttgcaaagct tgtttggctt tggcgcagaa
    22141 ttggcaacct ggtttagtga aaattgccac agactcttga gctttccaat caggagcaac
    22201 gaatttcagc atagtatctg catcggatac tttgaacgga tcgcctggtt cttcaggctc
    22261 gatgaacatt ttttcaacca cgccgtcgtt aaccagcatg gagtaacgcc aagagcgttt
    22321 accgaagccc aagtcttctt taccgaccag catacccata ccttcggtaa attcgccgtt
    22381 gccatcagga atcatgtaga tgttgtcgga ttcttcttcg gcagcccaag cgttcattac
    22441 gaacgtatcg tttacagata cgcagtagat tgcgtcaacg ccgttttctt tgaacgcgcc
    22501 gaacaattcg ttgtaacgcg gcaggtgtga agaagaacaa gtcggggtaa atgcaccggg
    22561 cagggagaat acgactactt ttttgccttt gaacaaatca tcggtagaca catctttcca
    22621 agtgtcgccg acgcgggtgc ggaatactac ggaaggtact ttttgaccgg tacgatcttg
    22681 caaagccatt ttaagctcct taatagattg gtttgagaaa taattgtgaa tcgctgggca
    22741 ttctacccca tgcaaaatga tttgtataat ttatagttca aataatataa attatttaaa
    22801 actatcaaac acaactccga tttgcctgtt tcccacatat aaagccgaat tagaaaaaaa
    22861 caagctgcaa tgaaaagatt ttccggcaac tgcgcccttg cgccgcaaat caggacaaaa
    22921 taccgtctga aaggctttca gacggtattt attcatacgg tctcggctta tttcgccaat
    22981 tcggcacgca atttgtgggt tacgttcatc atcacttgga gttgttccag agtttctttc
    23041 cagccgcgtg ttttcaggcc gcagtccggg ttaacccaca gacgttcaac cggtacaacc
    23101 tcgatggctt tgcgcaacag gtgctccact tcggcttctg tcggtacgcg cgggctgtgg
    23161 atgtcgtaaa cccccgggcc gatgtcgttc gggtatttga attcgccgaa cgcggtcaag
    23221 agttccatgt cggaacgtga agtctcgatg gtgatcacgt ccgcatccat tgcagcaatc
    23281 gcaggcagga tatcgttgaa ctcggagtag cacatatgag tgtggatttg tgtgctgtct
    23341 tcgcaaccgg cagaggacag gcggaaggat tcgcccgccc agttcaggta ggcatcccaa
    23401 tcggcgcgtt tcagcggcaa gccttcgcgg atggcaggtt cgtcaatttg gatgactttg
    23461 atgcctgctt tttccagatc caatacttcg tcgttcagag ccagtgcgat ttgtttgcac
    23521 acggtagagc gaggaatatc gttgcggaca aaagaccatt gcagaatggt tacagggccg
    23581 gtcaacatac ctttcatcgg gcgtttggtc aggctttgtg cgtaagtaga ccaggcgaca
    23641 gtcatggctt caggacggct tacgtcaccg aagataatcg gtggtttaac acaacgtgag
    23701 ccgtagcttt gtacccagcc gtattgggtg aatgcaaaac cgctcaacaa ttcgccgaag
    23761 tattcgacca tgtcgttacg ctcggcttcg ccgtgtacca gtacgtccaa atccagtttt
    23821 tcttgttctt caaccaccaa ggcgatttct tttttcatcg cggcttcgta atcggcggca
    23881 gacagttcgc cttttttgaa ggctgcgcgt gcttggcgga tttcggtagt ttgcgggaaa
    23941 gaaccgatgt tggtcgtcgg cagcagaggc aggttcaacc atgcttgttg cgctttgata
    24001 cggtcggcaa atggagattt gcgttggtct gcgttggcag gcaaatcggc caggcgtttg
    24061 gcaacgtctg cacggtggat ttcgctgctg ttggcgcgtg aatcggcagc agcttggctg
    24121 gcggccagtt cttcggcaac ggaatcgcga ccttcgttca atgcggcttt cagaacgcgc
    24181 aattcttggg ttttttgcag ggtaaatgcc aaccaagagt acaggtccgg tttgttggct
    24241 ttcagttttt cttcaactga caagtcaaat ggagtgtgca gcagagagca agagctggaa
    24301 atccacaaac ggtcgcccag tttggcttgc agaggctcaa cagtttccaa aactttgttc
    24361 aggttggcgc gccagatgtt gcggccgtcg atgatgcctg cagacagaac tttgtcgtag
    24421 tcggcaaatg cgtccagttg ctcgggagcg cgtaccaagt cgatgtgcag gccgtcaaca
    24481 ggcagggatt tcaacaatgc ggcgtgttcg gcaacagaac cgaagtaagt gctcagcagg
    24541 attttggcac ttactttgct caaagtagcg taaacgtctt tgtaggcttc tacccattct
    24601 ttaggcagat cgacagtcaa agcaggctcg tcgatttgaa tccactcggc accggcttca
    24661 accaaagcag tcaggatttc aacgtaaaca ggcaacagtt taggtaacag gctcagacgg
    24721 tcgaattcaa cggcgccttt ttctttacca acccacagga aagtcaacgg acccacaacg
    24781 gtcggtttgg ctttcaagcc caaagcttgg gcttcttgca gttgttggac gtagtgtttg
    24841 gcgttggctt tgaattcagt attggcgtgg aattcaggca ccaagtagtg gtagttggtg
    24901 tcgaaccatt tggtcatttc gatagcgaat tggtctttgt taccgcgcgc cagttggaag
    24961 aattgttcca aagacaggtt ttggctgtcg aagccgaagc gggcgggaat cgcgccggtg
    25021 gcgacttgca ggtcgaggat gtggtcgtag aaagtgaaat cgcctgcggc aacgtaatcg
    25081 gcgttggcag cagcttggtg tttccagttt ttctcgcgca agtctttagc aacagccagc
    25141 aattcttgct cgctgatttc tttgcgccag tatttttctt gggcgaattt caattcgcgg
    25201 aaggcgccga cacgcgggaa gcctgagaaa tgtaatgttg tcatgttgaa ctctccagtt
    25261 ggattttgta aggaccggtt caatccgaat cggccggtat tgtaaatcag aatggctagg
    25321 atgggggcgg cgttttgctt ttcagacggc ctcggatgcc gtctgaagcc gttttcagct
    25381 ttaagggcgc acgcctaaaa tatggcagat ggcgtaagtc agctcgctgc ggttaagcgt
    25441 atagaagtgg aaatctttca cgccttcgcg ggacaggact ttgaccatat cgatggcgat
    25501 acttgccgcc accagattgc gcgtaccttg gtcgtcgtcc aaaccttcat acatttgcga
    25561 cagccagctt gggattttga cgttggttac ttgagccatt ttaccgagct gcttgaagtt
    25621 ggtaacaggc aaaataccgg ggacgatttc cacatcgata cccaacatca cgcagcggtc
    25681 gcggaagcgc aggtagcgtt ctacgtcaaa gaaaaattgg gtgatgacgt ggttcgcgcc
    25741 cgcatcgatt ttgcgtttca aattaatcag gtcggcttgt gcggatttcg cttcgggatg
    25801 cacttcggga tatgctgcta cagagatgtc gaagtcggcg acggagcgta atagcttaac
    25861 caagtcttcg gcataaaacg gttttttctc gtaaccgggc ggctcgtcgc cgcgcagggc
    25921 gacaatgcgg cggatgccgc tgtcccaata gtctttggcg atttggcgca attcgtcggg
    25981 agaagcgtcg atgccggtca ggtgcggcgc ggcttccaag ccggtttctt gcttaatgcg
    26041 tttgacgatg ccgtgcgtgc ggtcgcgttc gccggaattt gcgccgtagg ttacggatac
    26101 gaacttggga tgcagggttt gcagacggtg gatggaatcc cacagcatcg tttccatttg
    26161 ttcgtttttc ggcgggaaaa attcaaacga aacgttgatg tcgcctttca aatcggaaag
    26221 gctgttgttt aacgcattga tttcttttgc gtaattcatg gctgcgtacc ttttattatc
    26281 ttttatatga tattttcagg caacataata aagttgcgcc aaacatgagt caatttcaaa
    26341 attttcagat ttatcatgaa aatttctaat atattacaaa aatagttaaa tttccttgta
    26401 caagccttct aaatttatga taaagtttgt tatgttataa caattcaact aaggcgatat
    26461 atgaaaccga atattcatcc tgacaattat cgaaccgtcc tttttttcga cagcagcgcc
    26521 aatgaaggct ggttaatccg ctcctgtgcc gaaacacacg gaaaaacgat ggtttggaca
    26581 gacggcaaag aatatccgct gttttcgctg gatacttctt ccgcatccca tcccgtctat
    26641 accggcaagc agcgcaacgt caacaccgaa ggccgtgcca gcaaattcaa ccaacgtttc
    26701 caatccgtca tgtcatcgtt tagaaaggac aaataatgca ggttctgtct tcactcaaaa
    26761 ctgccaaaca acgccaccgc gactgccaaa tcgtcagacg caggggcaaa gtttacgtca
    26821 tctgcaaaag caatccgcgt tttaaagcgc gccaacgctg accccctacc ccttcctgct
    26881 tcg
//
LOCUS       DAWHRA010000009        26504 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWHRA010000009 DAWHRA010000000
VERSION     DAWHRA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26504
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.009"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..579)
                     /locus_tag="WIB30_000290"
     CDS             complement(<1..579)
                     /locus_tag="WIB30_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase"
                     /protein_id="HEZ3796429.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLNAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPHSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKN"
     gene            complement(704..862)
                     /locus_tag="WIB30_000291"
     CDS             complement(704..862)
                     /locus_tag="WIB30_000291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796430.1"
                     /translation="MPSEALFALRTASPHHANGLNARNGFRPFFFRNRYKTENRRIRS
                     RPHIAFSF"
     gene            complement(875..1531)
                     /locus_tag="WIB30_000292"
     CDS             complement(875..1531)
                     /locus_tag="WIB30_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ3796431.1"
                     /translation="MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFVLFLLVFRLCWGIWGSDTARFSRFVQGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLILPMITGFKYIEGKTSIRFAGKAALAAALSVASLAAAAILLLS
                     "
     gene            1595..2485
                     /locus_tag="WIB30_000293"
     CDS             1595..2485
                     /locus_tag="WIB30_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ3796432.1"
                     /translation="MFPVFHLSGESRRQMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDTLPFAVIAGFPEIMLDTADSLAKRQD
                     INAWVVGCGLGTGRAAVGTLAGILTEHTDKPVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITKSM"
     gene            2755..4566
                     /gene="typA"
                     /locus_tag="WIB30_000294"
     CDS             2755..4566
                     /gene="typA"
                     /locus_tag="WIB30_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ3796433.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(4976..5066)
                     /locus_tag="WIB30_000295"
     tRNA            complement(4976..5066)
                     /locus_tag="WIB30_000295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5030..5032),aa:Ser,seq:gga)
     gene            complement(5105..5191)
                     /locus_tag="WIB30_000296"
     tRNA            complement(5105..5191)
                     /locus_tag="WIB30_000296"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5155..5157),aa:Leu,seq:cag)
     gene            complement(5227..5313)
                     /locus_tag="WIB30_000297"
     tRNA            complement(5227..5313)
                     /locus_tag="WIB30_000297"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5277..5279),aa:Leu,seq:cag)
     gene            5402..7777
                     /gene="rnr"
                     /locus_tag="WIB30_000298"
     CDS             5402..7777
                     /gene="rnr"
                     /locus_tag="WIB30_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ3796434.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPVHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDVIDADAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWISDGIDHPYKAQIDTLYKLFKILQKKRFERGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESRKKAKKPVPIKVKKRKGKS"
     gene            complement(8116..9579)
                     /gene="guaB"
                     /locus_tag="WIB30_000299"
     CDS             complement(8116..9579)
                     /gene="guaB"
                     /locus_tag="WIB30_000299"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ3796435.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(9726..12284)
                     /gene="glnD"
                     /locus_tag="WIB30_000300"
     CDS             complement(9726..12284)
                     /gene="glnD"
                     /locus_tag="WIB30_000300"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ3796436.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAALKTLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLIPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATNLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFNLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAVKTVKQLSGILTPMLRSRVSSAPMRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTKEESDLLAWLVENHLLMSTVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILFKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLT"
     gene            complement(12333..12653)
                     /locus_tag="WIB30_000301"
     CDS             complement(12333..12653)
                     /locus_tag="WIB30_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3796437.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            12669..12938
                     /locus_tag="WIB30_000302"
     CDS             12669..12938
                     /locus_tag="WIB30_000302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796438.1"
                     /translation="MVKVKHLLLRILLHIKAKKRGGGSHTQTHTHQEERGKIKTDWET
                     ANKQSRFHIGIIKQKPLSGIDSNQFRFYSQTEKEKRHIRLYGYAV"
     gene            complement(12978..13451)
                     /gene="bfr"
                     /locus_tag="WIB30_000303"
     CDS             complement(12978..13451)
                     /gene="bfr"
                     /locus_tag="WIB30_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ3796439.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(13479..13943)
                     /gene="bfr"
                     /locus_tag="WIB30_000304"
     CDS             complement(13479..13943)
                     /gene="bfr"
                     /locus_tag="WIB30_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ3796440.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVSRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            14298..14501
                     /locus_tag="WIB30_000305"
     CDS             14298..14501
                     /locus_tag="WIB30_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796441.1"
                     /translation="MPSEGLSDGMPARTDAAWSGCRFEGVFNAKGNLSYDVFAAGALK
                     KPGILGRSARLPGSISAIYSKPY"
     gene            14519..14986
                     /locus_tag="WIB30_000306"
     CDS             14519..14986
                     /locus_tag="WIB30_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEZ3796442.1"
                     /translation="MKIENIDIISPELFPQETFNETEAFGALVWLWAVSPIYQHAGVQ
                     EAAVNILPVLKNGQFALFSSNGHPVAYCTWAYFDEETEWQYLQSNDVLRHSENWCSGN
                     RMWLINWFAPFGDSRMMKRILVHLFPKREIRWLYHRGSEKGKRIMRFPALSKQ"
     gene            complement(15027..15383)
                     /locus_tag="WIB30_000307"
     CDS             complement(15027..15383)
                     /locus_tag="WIB30_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796443.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPPPDDSVYWKYSRIEQEYPAMM
                     SKNINLRIYSYEEYSKQIDDLFAKEKKDWKECGYDPIGGGGGSKADACMRKRGWYRVG
                     DDIYPENKKYEWPREN"
     gene            complement(15380..15718)
                     /locus_tag="WIB30_000308"
     CDS             complement(15380..15718)
                     /locus_tag="WIB30_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796444.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPNPWDAASFWELKNYANPYPGS
                     ASAALDQYPSKARRRQLKDMQECGYDPIDGGKSEADACLRKKGWCRKGFDPYPENKKY
                     EWPREEGKTK"
     gene            complement(15715..16077)
                     /locus_tag="WIB30_000309"
     CDS             complement(15715..16077)
                     /locus_tag="WIB30_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ3796445.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPPPDDSAFWRLTNYAKLYPGLT
                     SATLDQYPPEERRRQLHDSFAREKKDWKECGYDPIGGGGGSEADACMRKRGWYRVGND
                     IYPENKKYEWPREEGKTK"
     gene            complement(16074..22895)
                     /locus_tag="WIB30_000310"
     CDS             complement(16074..22895)
                     /locus_tag="WIB30_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ3796446.1"
                     /translation="MNKRCYKVIFNKKRSCMMAVAENVHRDGKSMQDSEAASVRVTGA
                     ASVSSARAAFGFRMAAFSVMLALGVAAFSPAPASGIIADKSAPKNQQAVILQTANGLP
                     QVNIQTPSSQGVSVNRFKQFDVDEKGVILNNSRSNTQTQLGGWIQGNPHLARGEARVI
                     VNQIDSSNPSLLNGYIEVGGKRAEVVVANPSGIRVNGGGLINAASVTLTSGVPVLNNG
                     NLTGFDVSSGKVVIGGKGLDTSDADYTRILSRAAEINAGVWGKDVKVVSGKNKLDFDG
                     SLAKTASAPSSSDSVTPTVAIDTATLGGMYADKITLISTDNGAVIRNKGRIFAATGGV
                     TLSADGKLSNSGSIDAAEITISAQTVDNRQGFIRSGKGSVLKVSDGINNQAGLIGSAG
                     LLDIRDTGKSSLHINNTDGTIIAGKDVSLQAKSLDNDGILTAARDVSVSLHDDFAGKR
                     DIEAGRTLTFSTQGRLKNTRIIQAGDTVSLTAAQIDNTVSGKIQSGNRTGLNGKNGIT
                     NRGLINSNGITLLQTEAKSDNAGTGRIYGSRVAVEADTLLNREETVNGETKAAVIAAR
                     ERLDIGAREIENREAALLSSSGDLHIGSALNGSRQVQGANTSLHNRSAAIESSGNIRI
                     ATKDLQNTNEHLRFHTEETHREHRIEYQAEGRTERYPEGSQKELGWEIFEDESLHMRT
                     PDGSPHSVWYKYDYERITAESKITESKPGQIISGGNLVLDAAKLKNHNSRIIAGGRLI
                     VGTPESALDNDETLGTKTITDKGDLHRYHRHHKKGRDSTGYSRSPYEPAPEVSSIRMG
                     ISAYKGYAPQQASDIPGTVVPVVAENGIHPTFTLPNSSLFAIAPNNKGYLIETDPAFT
                     DYRKWLGSGYMLAALQQDPNHIHKRLGDGYYEQKLVNEQIAKLTGYRRLDGYTNDEEQ
                     FKALMDNGITIAKELQLTPGIALSAEQVARLTSDIVWLENETVTLPDGTTQTVLKPKV
                     YVRARPKDMNGQGALLSGSVVDIGSGAIENRGGLIAGREALILNAQNIKNLQGDLQGK
                     NIFAAAGSDITNTGSIGAENALLLKASNNIESRSETRSNQNEQGSVRNIGRVAGIYLT
                     GRQNGSVLLDAGNNIVLTASELTNQSEDGQTVLNAGGDIRSDTTGISRNQNTIFDSDN
                     YVIRKEQNEVGSTIRTRGNLSLNAKGDIRIRAAEVGSEQGRLKLAAGRDIKVEAGKAH
                     TETEDALKYTGRSGGGIKQKMTRHLKNQNGQAVSGTLDGKEIILVSGRDITVTGSNII
                     ADNHTILSAKNNIVLKAAETRSRSAEMNKKEKSGLMGSGGIGFTAGSKKDTQTNRSET
                     VSHTESVVGSLNGNTLISAGKHYTQTGSTISSPQGDVGISSGKISIDAAQNRYSQESK
                     QVYEQKGVTVAISVPVVNTVMGAVDAVKAVQTVGKSKNSRVNAMAAANALNKGVDSGV
                     ALYNAARNPKKAAGQGISVSVTYGEQKNTSESRIKGTQVQEGKITGGGKVSLTASGAG
                     KDSRITITGSDVYGGKGTRLKAENAVQIEAARQTHQERSENKSAGFNAGVAIAINKGI
                     SFGFTAGANYGKGYGNGDETAYRNSHIGSKDSQTAIESGGDTVIKGGQLKGKGVGVTA
                     ESLHIESLQDTAVFKGKQENVSAQVTVGYGFSVGGSYNRSKSSSDYASVNEQSGIFAG
                     GDGYRIRVNGKTGLVGAAVVSDADKSKNLLKTSEIWHKDIQNHASAAASALGLSGGFS
                     YSPKPTSGQYSTKKEAEIGKIGGKPVSLMRFDQVSAKDDELNEKYRSERIEKGETFKE
                     ANLNQNNAGGLKFGLKQNDIHSNDKYALAKMGLGNLLGNAKESESRQSITRSVISEGD
                     WQIASAQGRKNIAGIEKGTSSAHKALAKADREGLLKEVELNRDVAKEFINETLIGGIA
                     DEAYRSQFIAEHRLMTFKMDENGEPIEDKQLEEDINKQFDNSVKLKKEFASFKDYWEA
                     YKAIGGNIYELREVSDQERKNLKTARYTDPETGKTVEKIVVGVNGIFNNIQAAAKFAA
                     QQYVGRFNPEKNRYERTYENVYFLHNPETNGRGFSKLPEIAVAAFHKMLEGAKIGNKT
                     VIGLSNSGLALGNIMEDYGKDKNGLFVGSHSRGTLVVDNVLNTLNTQANRDKKILSNT
                     ELKMVGPAANVVRADKRLFQLQQGVTTPRTADFARQSIQIENHELDLIGMLIGRNPAT
                     VGTNTRQKSQWQAIRDIIGDYTSPHNCYGMANKQCVTDGYRDPENKQTQSPTGVFERG
                     VSNEIEIMYRPVRIYDLQHPKGKTK"
     gene            complement(23405..24388)
                     /gene="lipA"
                     /locus_tag="WIB30_000311"
     CDS             complement(23405..24388)
                     /gene="lipA"
                     /locus_tag="WIB30_000311"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ3796447.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(24381..25004)
                     /gene="lipB"
                     /locus_tag="WIB30_000312"
     CDS             complement(24381..25004)
                     /gene="lipB"
                     /locus_tag="WIB30_000312"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ3796448.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(25006..25281)
                     /locus_tag="WIB30_000313"
     CDS             complement(25006..25281)
                     /locus_tag="WIB30_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ3796449.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            25466..>26504
                     /locus_tag="WIB30_000314"
     CDS             25466..>26504
                     /locus_tag="WIB30_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ3796450.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQK"
BASE COUNT         5706 a         6502 c         7337 g         6959 t
ORIGIN      
        1 atttttaacg agggcggcaa aacggccgtt gaactgcttt tcccaaaaac ggcggcgttc
       61 ggtaacggat ttcagtttgg ctttgaccgc atcgcgccat cttccggaga tttccgccat
      121 atcgcccaaa gaatgaggca gcagggcttc cagcctttca cgcagcagtc gggcgaggac
      181 gggcgcgctg ccggagctgg aaacggcaat ctgaaccggg ttgcggtcga taaccgacgg
      241 gaagatgaag ctgcaatggt cgcggtcgtc caccacgttg accggctttt ggcagctttc
      301 ggcaagatgg aaaacgcgcc ggttgagggc ttggtcgctg cttgccgcaa tgatgaggaa
      361 aaccgtgcgg atgtgttcgg cacgaaattc ttcggcaagc cacaggattt tgttttccgc
      421 cgccaacgcg gagagttcgg cattcaggtg ttttgcggca accctgacct ctgcgcccgc
      481 attcagcagc aggctgattt tgcgtgcggc gactgcgccg ccgcctacga ccaatacggg
      541 gcggccggcg aggttggcga aaatggggaa ataattcact ggctgactcc tttgctgttt
      601 gcccgcacct tgtttccgat acggtgcgtc gcggcatttt tgtcggaacg cgagtcattt
      661 tagacaaaag aatttccccg gttaaataat aaaaaggtat ttgttagaag ctgaaagcta
      721 tatgggggcg gctgcggatg cggcggtttt ccgttttata acggtttcgg aagaaaaacg
      781 gcctgaagcc gtttcgggca ttcagaccgt ttgcgtggtg aggggatgcc gtccgaaggg
      841 cgaaaagggc ttcagacggc attgatgtcg ggtttcagga caggagcagg atggcggctg
      901 cggcaagcga ggcaaccgat aatgcggcgg caagcgcggc tttgcctgca aagcggattg
      961 aggttttgcc ttcgatgtat ttgaagccgg ttatcatcgg gaggatgagg tttttctttt
     1021 tgaatacgcg gtatgcggcg acggcggcga tgtggattgc agaaaaaacg gcgagcagct
     1081 tgaaaaagtt gaggtggatt ttccgcataa ggctgcccgt atgttcggaa accaaatggt
     1141 tgaggtagcc gttggtgctg aaggtgtttt catcggcggc aaaaagcccg gtgccgactt
     1201 ggaaggacac ggcggccaaa agcgcaacga ccatcagtgc gcccaagggg ttgtgtccgg
     1261 gctggatgtg ttcgggaata ccgtttttca gatagccgcg tatgcctgcc cagccttgga
     1321 cgaaacggga aaaacgggcg gtatcgctgc cccaaatgcc ccagcagagg cgaaatacga
     1381 gcaggaaaag gacgaacagc ccgacgcgcg tgtgccattg cagcatatcg ccgccggctt
     1441 tcgcgctata ccacataaag ggcagggacg cggcaagcag ccagtggaaa aggcgggtgg
     1501 ggaggtccca gactttggtt ttgtttttca taatcggttt ccggcggtag aatcggtttg
     1561 ttttcgagcc ttattttaac cgattggagg ggcaatgttt cccgtttttc atctttcagg
     1621 cgagagccgc cgccagatgc ttcagacggc attgcgtttt ccccatgttt tcaaagcccg
     1681 tgcggaagat tcgcacaaag ggactttcgg cacgctcgcc gtagtcggcg gatcggcagg
     1741 gatgagcggc gcgcccgtat tggcggcatc ggcggcaatg tatctcggct gcggcaaagt
     1801 gtgggcgggt ttcaatcagg atacgctacc ttttgccgtt attgccggtt ttcccgagat
     1861 tatgctggat acggcggaca gtttggccaa acgtcaagat ataaacgcct gggttgtcgg
     1921 ttgtggattg ggtacaggta gggcggcggt cggaacgctt gccggaattt tgacggaaca
     1981 cacggacaag cccgtcgttt tggatgcgga tgcgctgaac atattatcaa ccgatgccga
     2041 aacccgaaat ctggcgcgcg ggtgtaaaaa cctgatttta acgccacacc ccgccgaagc
     2101 cgcgcgcctg cttggaacga cggttgcgca ggttcaggcg gatcggacgg cggcagtgag
     2161 gaagataggg gcaattttcg gcgcaaccgt ggttttaaag gggcacaaaa cattggttgc
     2221 ctcacccgat acggaaatct atgtcaacga aagcggcaac gcgggattgg caacggcggg
     2281 cagtggcgac gtattgggcg gcatcatcgg cagtctgctc gcacagggcg tgccggtttt
     2341 tgaagccgcc tgcgcgggcg cgtggctgca cggcgcggcg gcggatgtca taaaagaatc
     2401 ggcaggcatt gcggcagggc tgttggcagg ggaaatcgct ccggcggcaa ggtggctgcg
     2461 caaccggata actaaaagta tgtaagaaga tatagtggat taacaaaaac cagtacatcg
     2521 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg
     2581 gttccgtact atttgtactg tctgcggctt cgtcgccttt tcctgatttt tgttaatcca
     2641 ctataccgtc tgaaaggcaa gggcttcaga cggcatcttc atttcccaaa tactgtccgg
     2701 taaagcgtgg tataatgcgt agttatctct tacagttttt gaaaaacatt aattatgaaa
     2761 caaatccgca acatcgccat catcgcccac gtcgaccacg gcaaaaccac attggtcgac
     2821 caactgctgc gccaatccgg cacattccgc gccaaccagc aggttgacga gcgcgtgatg
     2881 gacagcaacg accttgaaaa agaacgcggc atcaccatcc tcgccaaaaa caccgccatc
     2941 gattacgaag gctaccacat caatatcgtc gacacgccgg gacacgccga cttcggcggc
     3001 gaagtagagc gcgttttggg gatggtggac tgcgtcgtct tgttggtgga cgcgcaggaa
     3061 ggcccgatgc cgcaaacccg tttcgtgacc aaaaaagcct tggctttggg gctgaaaccg
     3121 attgtcgtca tcaacaaaat cgacaagccg tccgctcgtc cgagctgggt tatcgaccaa
     3181 actttcgagc tgttcgacaa cttgggcgcg accgacgagc agttggattt cccgattgtt
     3241 tacgcttcag ggttgagcgg tttcgccaaa ttggaagaaa ccgacgagag caacgacatg
     3301 cgtccgctgt tcgatactat cttaaaatat acgcctgcac cgagcggcag cgcggacgaa
     3361 acgctgcaac tgcaaatttc ccaactcgac tacgacaact acaccggccg cctcggtatc
     3421 ggtcgtatct tgaacggacg catcaaaccc ggccaaaccg ttgccgtcat gaaccacgat
     3481 cagcaaatcg cccaaggccg catcaaccag cttttgggtt tcaaaggttt ggaacgcgtg
     3541 ccgcttgaag aagccgaagc cggcgacatc gtgattattt ccggtatcga agacatcggt
     3601 atcggcgtaa ccatcaccga caaagacaat cccaaaggcc taccgatgtt gagcgtggac
     3661 gaaccgacgc tgacgatgga ctttatggtc aacaccagcc cgctggcggg tacggaaggc
     3721 aaattcgtaa ccagccgcca aatccgcgac cgcctgcaaa aagaattgct gaccaacgtc
     3781 gccctgcgcg tggaagatac cgccgatgcc gacgtgttcc gcgtatccgg gcgcggcgag
     3841 ctgcacctga ccattttgct ggaaaacatg cgccgcgaag gctacgaact cgccgtcggc
     3901 aaaccgcgcg tcgtgtaccg cgacatcgac ggtcaaaaat gcgaaccgta tgaaaacctg
     3961 accgtggatg tacccgacga caaccaaggc gcggtaatgg aagaactcgg ccgccgccgt
     4021 ggcgaactga ctaatatgga aagcgacggc aacggacgca cccgcctcga ataccatatt
     4081 ccagcgcgcg gcttgatcgg tttccaaggc gaatttatga ccctgacgcg cggggtcggg
     4141 ctgatgagcc acgtgttcga cgattacgcg cccgtcaaac ccgatatgcc cggccgccac
     4201 aacggcgtgc tggtgtccca agagcagggc gaggcagtcg cttacgcctt gtggaatctg
     4261 gaagaccgcg gccgtatgtt cgtatcgccc aacgacaaaa tctacgaagg catgattatc
     4321 ggcatccaca gtcgcgacaa cgatttggtg gtcaacccgc tcaaaggcaa aaaacttacc
     4381 aacatccgtg ccagcggtac cgacgaagcc gttcgcctga ccacgccaat caagctgacg
     4441 ctggaaggtg cggttgagtt tatcgacgat gacgaactcg ttgaaatcac gccgcaatcc
     4501 atccgtctgc gcaagcgtta cttgagcgaa ttggaacgcc gccgccactt taaaaagctg
     4561 gattgatgtt tactggatta atgtttaaat gatacgcgat gccgtctgaa aaatttcaga
     4621 cggcattttt tattcggacg ggctttgcgg cttcttgaag ctgtttcaga cggcgttttt
     4681 cctacccaat caagaaactg ccgccatttt tccagcggta tatcgccccg tcgcgtttcg
     4741 gtatcgggtt cggcttcccg gcagcatgtc gaacaatgcc gtttgaagga atacgccttt
     4801 tgagtcctta caggctgagg aagagggtaa aacataccgc aaaacatacc acaaaaaacg
     4861 gtaacggata gttgtaagcg gttgatgacg attatagaca ataacgggtt ttcccaatga
     4921 aattattgtt tgaataataa aaaatcccaa accgtaaaag tttgggattt gtatttggca
     4981 gagaggaagg gattcgaacc ctcgatacgc tattcacgta tacacgcttt ccaggcgtgc
     5041 gacttaaacc actcatccac ctctctaatg gcggaaatta tcccaatcgg gataatttat
     5101 tatttggtgc ccgggagaag actcgaactt ccacacccat gaggatacca gcacctgaag
     5161 ctggcgcgtc taccaattcc gccacccggg caatctaaat attgaaataa agcaaagcat
     5221 ttgatttggt gcccgggaga agactcgaac ttccacaccc gtgaggatac tagcacctga
     5281 agctagcgcg tctaccaatt ccgccacccg ggctttgctt tatttgctgt tttgcggtac
     5341 agtgtcctgc cgcaaagaag tcgctattat atagttcata aagagaatgt caacagtcca
     5401 aatgaataaa aatattaaat ctttaaattt acgggaaaaa gacccgtttt taagtcgtga
     5461 aaaacagcgt tatgaacatc ctttgcccag tcgggaatgg ataatcgaat tgttggagcg
     5521 caaaggtgtg ccttcaaaaa tcgaatcgct tgcgcgcgag ctgtcgatta cggaagacga
     5581 gtatgtcttt tttgaacgcc gtctgaaggc gatggcgcgg gacggtcagg ttttaatcaa
     5641 ccgtcggggc gcggtttgcg cggcggacaa attggatttg gtcaaatgcc gcgtcgaggc
     5701 gcataaggac ggtttcggtt ttgccgtgcc gctcacgccc gccaaagacg gtgattttgt
     5761 tttgtatgaa cgccagatgc gcggcattat gcacggcgat attgtcactg ttcgtcctgc
     5821 cggcatggac cgtaggggcc gccgcgaagg gacagttctg gatattgtcg aacgcgcgca
     5881 aagcaaagtg gtcggccgtt tctatatgga taggggcgtg gcgattttgg agccggaaga
     5941 caagcgtctg aaccaaagca tcgtattgga accggacggc gtggcgcgtt tcaaacctga
     6001 atccggtcag gtcatcgtcg gcgaaattga ggtttatcct gagcaaaacc ggccggcagt
     6061 ggcaaaaatc atcgaagttt tgggcgatta tgccgacagc ggcatggaga ttgaaattgc
     6121 cgtgcgcaag catcatttgc cgcaccaatt cagtgaagcg tgtgccaaag ctgcgaaaaa
     6181 aattcccgtc catgtacgca aaagcgattt gaaaggccgc gtcgatttgc gcgacctgcc
     6241 tttggtaacg atagacggcg aaacggcgcg cgatttcgac gacgcggtgt ttgccgaaaa
     6301 agtcggacgc aattaccgtc tggtcgtggc gattgcggat gtcagccatt atgtccgccc
     6361 tgacgatgtg attgatgcag atgctcaaga acgcagtacc agcgtatatt tcccgcgccg
     6421 tgtgattccg atgctgccgg aaaacctgtc taacggcatt tgctcgctca atcccgatgt
     6481 cgagcgtttg tgtatggtgt gcgatatggt cgttacctat gcgggcaata tcaaagaata
     6541 ccgcttctac cccgccgtaa tgcgctctca tgcccgcctg acctacaacc aagtttggaa
     6601 atggatttca gacggcatcg accatccgta caaagcccaa atcgacaccc tttacaaact
     6661 cttcaaaatc cttcagaaaa agcgtttcga acgcggcgcg gtggagtttg aaagcgtcga
     6721 aacccagatg attttcgatg acaacggcaa aatcgaaaaa atcgtccccg ttgtccgcaa
     6781 cgatgcccac aagctgattg aagaatgtat gctggcggcg aatgtttgcg cagcggattt
     6841 cctgttgaaa aacaagcata cggctttgtt ccgcaaccat ttgggcccca cgcccgaaaa
     6901 actcgccacc ctgcgcgagc agctcggtct gttggggctt caacttggcg gcggcgacaa
     6961 cccgtcgccg aaagactatg ccgcgcttgt cgaacaattc aaaggcagac ctgatgccga
     7021 attgctgcaa gtcatgatgt tgcgctccat gcagcaggcg gtttacgaac cgcattgcga
     7081 cggacacttt ggtcttgcct acgaagcata cgcccacttc acctcgccca tccgccgcta
     7141 tcccgacctg accgtacacc gcgccatcaa agccgtgttg aatcagcaaa cctacacgcc
     7201 aaaaaaaagc tggcaggctt tgggcgtgca tacctcgttc tgtgagcgcc gtgccgacga
     7261 cgccagccgc gacgtggaaa actggctgaa aacctattat atgcgcgata aggtcggcga
     7321 agtattcgaa ggtaaaatct ccggcatgac cagttttggt atctttgtaa cactggacgg
     7381 catccacatt gacggcttgg tgcatatcag cgatttgggc gaagactatt tcaacttccg
     7441 ccccgaaatc atggcaatcg aaggcgaacg cagcggcatc cgtttcaaca tgggggacag
     7501 ggttgccgtc cgggtcgccc gtgccgattt ggatgacgga aaaatcgatt ttgtcctgat
     7561 tgccgggggg agcggcaggg ggcggaaagt taaatcatcc gcgtctgcca aaccggcagg
     7621 gacggcgggg aaagggaagc cgaaaaccgc cgccgagaaa aaaacagccc gaggcggcaa
     7681 agtaagggga aggggcgcgt ctgccgccgc agaatcgagg aaaaaggcaa agaaaccggt
     7741 tccgattaag gtaaaaaaac ggaaaggcaa atcataatgc tgacggggcg gcttggggag
     7801 ggcggggcat actttcccga agccgccgcc cgattttttg atatggcggc cggcgcatca
     7861 tttcaggcca atcgaaacgc ccgtattgaa agattgcgtt tatttccacc gccgttttaa
     7921 aggccggcgg tattcggcag acggggcgca aacggcgttc agacggcatt ttcattcttt
     7981 cggcgtgtcc gtccgaatgg ctttgcccgt ccgcgcaatc agcccgtgcg gcttgccttg
     8041 aacggacaaa aaatgccgtc tgaaacccga aaatcaggtt tcagacggca ttttgccttg
     8101 aaaaggctgt tcaaatcagc ggtggtagtt cggtgcttct ttggtaattt gaacgtcgtg
     8161 aacgtgcgat tcgctcatac ctgcggaagt gatttccaca aattctgctt tttcgtgcat
     8221 ttcggcaata ttggcgcaac ccaaataccc catgctggag cgcagtccgc cggtcagttg
     8281 gtggatgatg ttcacaatcg ggcctttgta gggaacgcgg ccttcgatgc cttcggggac
     8341 gtatttgtcg gtgctgtcgg ttttgtcttg gaagtagcgg tcggcagaac cttggctcat
     8401 cgcgcccaat gaacccatac cgcgatagga tttgtacgag cggccttggt agagttcgat
     8461 ttcgcccggc gcttcttccg tgcctgcaaa cataccgccg agcatgacgc tgtacgcgcc
     8521 tgcggcgagg gctttggcga tgtcgccgga gaagcggatg ccgccgtcgg caatcagcgg
     8581 aacgcccgtg cctttgaggg cttcggcaac gttgtgaatg gcggtcagtt gcggcacgcc
     8641 gacacctgcc acgatacggg tggtgcaaat cgatcccgga ccgataccga ctttgacggc
     8701 atccgcgccg gcggcgacca aatccaatgc ggctttggca gtggcgatgt tgccgccgat
     8761 gacttggatg tgcggatagg tttctttcac ccatttcacg cggtcgatca cgccttggct
     8821 gtgcccgtgg gcggtatcga cgacgatgac atcgactcct gcttctacca atgcttttac
     8881 gcgctcttcg gtgtcgccgc cggtgccgac tgccgcaccg acgcgcagac ggccctcgga
     8941 gtctttgttg gcattgggaa attcggtggt ttttaaaata tctttgacgg taatcagacc
     9001 tttgagttcg tctttttcgt tcagaaccaa aacgcgctcg actttgtgcg tgtgcatcag
     9061 ttcgcgcgct tcgtctatgc ttgtgccttc ggggacggta accagacgtt cgcgcggggt
     9121 cataatggcg gaaacgggca aatcgacgcg gttttcaaaa cgcaggtcgc ggttggttac
     9181 gatgccgacg actttgccgt tttcaacgac gggcaggccg gacattttgc gtttgcgctg
     9241 cgcgcgcatt tccaagactt cgcggatgag cgtcgtcggc gcaacggtta cggggtcttt
     9301 gaccacgccg ctttcgtggc gtttcacttt ggaaacggcg cgcgcctgca tctccggcgg
     9361 catattttta tggatgatgc cgatgccgcc ttcctgtgcc atcgaaatgg cgaggcgcgc
     9421 ctcggtaaca gtatccatcg cggcggaaag cagggggagg ttgagcgtga tttcgcgggt
     9481 gagcttggtt tgaagtttaa cgtctcgcgg cagcacggtc gaatgtgcgg gaaccaacaa
     9541 aacatcgtcg aaagtatagg ctttttctac gatacgcata atgctcggtc tttcagtttg
     9601 tgcaagatgc acggcattat agcacgttac cggcggcttg acagtttata ttgtttgcaa
     9661 tatcccccgc cttcagacag cgtacggcgc ggcggcggga taccgtctga aaaccggcag
     9721 ggtgcttatg tcaactgttc cagcaaagcc tgcttcaatg aggactggat ttttgggttt
     9781 ttcaggtcgg ggctaaaaac ggtaaaactg tcttcggcgc gttcatccag tgtggagatt
     9841 ttggcatagc gcaggctgac gttgtgggcg aaaaagactt ccgccatatc ggcgagcagg
     9901 aaggggcggt tgacggcggt aatttcgacg gagtaccagt ccggatagtc ttcttcgggg
     9961 gtgatggtga tgcttggcgc gatcggcatg tagcggctgc ggcggctgat gcggcggcta
    10021 tggctggaga tttcggcaac ggtgtgtccg tggataaagc tgttgagttc ggcttcgagc
    10081 gcgctttgga tgtcggggta gtcttcgggg gcgtgctgcg agggaatttg cacgatgaag
    10141 gtgtcgagga tgtagtcgtg ttcggtgata aaggcgcggg cggcgagaat gtcgaagccg
    10201 tggcggctga agatacggca gagacgggca aacaagcgcg gcccgttggg catgaaaacc
    10261 ataacttgaa agctgtcgct tttgaacagg atgcggctgc ggacgatggg ggtttcaaaa
    10321 tcgtggacta ggttggcggc gtgccacagg atttcgcggg actggtggcg ggcgaagtag
    10381 gcggaaccga gcgcgttcca tagttttttc tgctgttttt cggggacggc ggcgcgggtg
    10441 agtaagtcgg cggcttcctg ccggcggcgg ccgaagaggg tgtgcgggtt gccgccgttg
    10501 cctgtaaggt agcgtccggc ggcatggaag aggctttcca gcaggctggc gcgccaggcg
    10561 ttccatagtt tgggattggt gccgcgtatg tcggaaatgg tcaggaggta gagtgcgctg
    10621 aggcgttcgt gggtttgcac gcgtttgcag aaggcatcga gtacgtcggg gtcttggatg
    10681 tcttcttttt gggcgacggt ggacataaga agatgatttt cgaccagcca ggcgagcagg
    10741 tcgctttctt ctttggtcag gaagtggtcg gcggcaaatt ggcgtgcgtc cgcgatgcct
    10801 tgtatggcat ggtcgccgcc gcgtcctttg gcgatgtcat ggaaaaaggc ggcaaggtag
    10861 aggatgtctt gtttttcaaa ggactgcatc agtgcagagg cgtagggcag ctcgtggctg
    10921 tgcatatcca aggcaaggcg gcggacgttg cggacgacgg tgaggatgtg gtcgtccacg
    10981 ggatagatgt ggaacaggtc gtgctggagc aggccgacga ttttttccca cgcgggcagg
    11041 tagcgtccca atacgccata gaggttgagg aagcgcaggg tttgggtcag tccgttgccg
    11101 ttgcggaaaa agccggcgaa gcggtggcgg ttttcagaat tttggtagaa gctgcggttg
    11161 attttgcgcg tcgcgcccca ccatgcgcgc agggtttgcg gttcgagcgc ggtaatatcg
    11221 ttgcgctgct gcatgatttc gacgattttg aaaatgtgtt cgggccgtct gaaaaaaata
    11281 tcggtgtgcc gcgcggcgat ttggttgttg acttggatgt agtcgtcgtc aatccgcagg
    11341 gtaacgcgca tcggcgcgga ggaaacgcgg cttcgcagca taggcgtgag gatgccgctc
    11401 agttgtttga cggttttgac cgcacggtaa aacacgcgca tcagttcttc gctttggcgg
    11461 cggaggttca agccttcata acccatgctt tcggcgactt gcggctgcaa attgaacagc
    11521 aggcggtctt cggcgcgctt ggcgtttaaa tgcagatgga tgcggatgtg ggcgaggcgg
    11581 cggtagccgt gcgaaagcat accggcttcg gcacgcgtca aaatccgctg tttgagcagg
    11641 tcgggcaggt tggtcgccaa gccttgtgct ttcgctatcc aaagcagggt gtggatgtcg
    11701 cgcagaccgc ccggacagct tttgatgttt ggttccaata ccgcccctga gccttgcgat
    11761 ttggcgtggc ggtgttccat ctccaccagt tttgcctcga caaacgccgc cacattgcgc
    11821 tgcgcgttca ttttttccgc tagtttatcc gccgtttggc ggttgccaaa caaaaaccta
    11881 gcctctaaaa acgctgtgtc ccccgtaata tcattgcgca cgctttcaca tagttcatca
    11941 acgctgccgc tttttacaga cggtatcagt ttgcagtccc acagggtttg gacaaaccgg
    12001 gcaatctgtt cctgaatgcc gtctgaaagc ggggcagggg agacaaccgc caaatccaca
    12061 tccgaacagg gatacagttc gccgcgtccg aagccgccta ccgccatcag gcataacgcg
    12121 ctgttttgaa aatactctgc ccacaatgcc gccaacaagg ttttgagtgc cgccgtatat
    12181 tccctgaaaa ataccgacac gcggttggct ttcaaataat gcgcttcggc ggcatcgcgc
    12241 tgctgtttga aggtttccag tgctgaagac aggtttgcag gcatttttat tctttcgatt
    12301 ggcgggaaaa ggggaggcgg atggttcggc ggtcaaatac cgctttcaga cggcatttgt
    12361 cgggtatcgg cggaattggt catcaggttc aaccgttcct gcggcggcag cagcaactga
    12421 tacgccgcca cgcccaaagc cgccgccatt ttttcgatat tcgacagggc gatgttccag
    12481 cgtttgcgtt cgactgccga cacataagtc ctgtccaaac cgcattgccg cgccaattct
    12541 tcttgcgacc aacccttgtt cacgcggaaa agccgcatat tgtatgccaa taccgcccgc
    12601 aaatcctgtt cgtcaggcag ttcggcaggc agagtcaatt tgttgcccat cattatgttt
    12661 ccggataaat ggttaaagtt aagcatttgc tgttacggat tttacttcac ataaaagcca
    12721 aaaaaagggg tggcggcagc cacacccaaa cacacacaca tcaagaggaa agaggtaaaa
    12781 tcaagacaga ttgggaaaca gcaaacaagc aatctagatt tcatatcggt attatcaaac
    12841 aaaaaccctt atcaggtatt gattcaaatc aatttagatt ttattcgcaa accgaaaaag
    12901 aaaaacggca tatccgttta tacggatatg ccgtctgatg gtgcggggga ctgctgtatt
    12961 tggcagtagt ttgtgtttta gtcctcttgc gccgctgttt gcaggtagtt gggcagaccg
    13021 attttgccga taagctcttg ctgggtctcc agccagtcta tatgttcttc gttggtgtct
    13081 ttttgttttt ccaacaaatc gcggctgacg taatcctgtt gcgcttctgc tgtggcgatg
    13141 gcggcaagca gggcttcgtg tttttcctgt tctttggtca aatcgcaggc gatgatttct
    13201 tcggtggact cgccaatcag aagcttgccc agttcttgca ggttcggcag accttcgagg
    13261 aacagaatgc gctcgatcaa atcgtcggca gctttcattt caacgatgga ctgtttgaag
    13321 aaatgttcgc ccagttcttc aaagccccag tttttcaaaa tacgggcgtg aaggaaatat
    13381 tggttgatgg ttaccagcag caagcctaag tttttgttca gctcgcggat aaccaaacgg
    13441 tcgcctttca tacggactcc ttttattccg aatacggatt acagttggct ttggtagtag
    13501 ttgccttcgc caatcagctc gatcaggcgc agctgctgtt ccagccagtg ggcgtggtct
    13561 tcttcggtat ctttcagttg ggcgaccatc aaatcgcggc tgacatagtc ttgcgcctct
    13621 tcgcacagtt taatgccttt tttcaaagcg tcgcgcactt cgtattcggt ttgcaggtcg
    13681 gctttgaggc aggaaaccac gtccgtgccg atattcagct cggcgcgcgc cattttcggc
    13741 gtaccgccca gcatcaggat gcgtcggatg aagtcttcgg cgtgtgtggt ttcttcttcc
    13801 atctcgtgat tgagacgttc aaaaagtttg gtgtagcccc attcggagta gaggcgggag
    13861 tggataaagt attggtcgcg tgccgccagc tcgccagaca gcaattcgtt catataatca
    13921 acaacagctt gattgccttg cataatatct ctctttccgt aacttggttt tcggtatgtc
    13981 tgaacagcat ctgtgtcgct attcgggatg cgtgcattgt atgcaaaagc tgtcggcatg
    14041 acaaattttc tatttaaaat acaaacaatt atcaaaagaa atagggcgta ttccccgatg
    14101 ctttccaaat cagtatgctg tttcttatcg gtttttttgc tgctaaaact aagaatccat
    14161 ctcatccata aagaacattt acacttatta accatagcaa aaaacaaata gagcacggtt
    14221 ttttatcaaa atttataatg aatcgttctc attaaccgac agattctttt gagattatcg
    14281 gattttggga ataaattatg ccgtctgagg ggctttcaga cggcatgcct gcccggacag
    14341 acgctgcttg gagcggctgt cggtttgaag gtgtgtttaa tgcgaagggc aatctgtcgt
    14401 atgatgtgtt tgcggcaggg gctttgaaga agcccggtat tttaggacga agcgcgcggc
    14461 tgccggggtc aatatccgct atttattcca aaccttatta aaacttaaat ttaaaatcat
    14521 gaagatagaa aatatcgata ttatttcgcc ggaactgttc ccgcaggaaa cattcaatga
    14581 aaccgaagca ttcggcgctt tggtatggtt gtgggcagtt tcgcctattt atcagcatgc
    14641 cggcgtacaa gaagccgccg tcaatatctt gccggtattg aaaaacgggc aatttgcctt
    14701 gttcagcagc aacggacacc ccgtcgccta ctgcacttgg gcttatttcg atgaagaaac
    14761 cgaatggcaa tatctccaat ccaatgatgt tctgcgccac tcggaaaact ggtgcagtgg
    14821 aaacagaatg tggcttatca actggttcgc acctttcggc gacagccgta tgatgaaaag
    14881 aattctggtg catctgtttc cgaaacgtga aataaggtgg ctgtaccacc gggggagtga
    14941 aaaaggaaaa cggattatga ggtttccagc gttgtcgaaa caataaaaca aggctgcctg
    15001 aaagtttccc ttcaggcagc cttgtttcag ttctctcgag gccattcgta ttttttgttt
    15061 tcgggataaa tgtcgtcacc tacacgatac caacctcttt ttctcatgca ggcatctgct
    15121 ttacttcctc ctcccccacc tattgggtca tagccgcact ctttccagtc ttttttttcc
    15181 ttagcaaaaa gatcatctat ttgtttgcta tattcttcat aagaatatat gcgtaaattg
    15241 atatttttac tcatcattgc cggatattct tgttctatac gggaatactt ccagtatacc
    15301 gagtcatcgg gcggaggttt gaatccccca aacgaacagg cagccaatcc catagccaaa
    15361 gagattgata cgatatattt cattttgttt ttccttcttc tcgaggccat tcgtattttt
    15421 tgttttcggg ataagggtcg aaacccttac gacaccagcc ttttttcctc aggcaggcat
    15481 ctgcttcaga ctttccgccg tctattgggt catagccgca ctcttgcatg tctttcagtt
    15541 gccttcgtct tgctttcgat ggatattggt caagtgccgc cgaggctgat cccggatagg
    15601 gattggcgta atttttcaat tcccaaaatg acgcggcgtc ccacggattt ggtttaaatc
    15661 ccccaaacga acaggcagcc aatcccatag ccagagagat tgatacgata tatttcattt
    15721 tgtttttcct tcttctcgag gccattcgta ttttttgttt tcgggataaa tgtcgttacc
    15781 tacacgatac caacctcttt ttctcatgca ggcatctgct tcactcccac cgcccccacc
    15841 tattgggtca tagccgcact ctttccagtc ttttttttct ctagcaaaag aatcatgcag
    15901 ttgtcttcgt ctctcttcag gtgggtattg gtcaagtgtc gctgaggtta atcccggata
    15961 aagtttggcg taatttgtca atctccaaaa tgccgagtca tcgggcggag gtttgaatcc
    16021 cccaaacgaa caggcagcca atcccatagc cagagagatt gatacgatat atttcatttt
    16081 gtttttcctt tcggatgttg cagatcataa atgcgtacag gacggtacat aatttcgatt
    16141 tcgttggaca ctcctctttc aaatacacct gtcggacttt gggtttgttt attttcaggg
    16201 tctcgataac catctgttac acattgttta ttagccatac cgtagcaatt atgcggcgag
    16261 gtgtaatccc ctataatatc ccttatagcc tgccattggc ttttttgccg ggtattggtt
    16321 ccgactgttg ccggatttct gccaatcagc atgccgataa ggtctaattc gtggttttct
    16381 atttggatgg actgacgggc gaaatctgcc gttctgggtg tcgttacccc ctgctgcagt
    16441 tgaaacagcc ttttatctgc ccggacaacg ttggcggcag ggccgaccat tttcagttcg
    16501 gtattggata aaattttctt atcccgattg gcttgggtat tcaaggtatt gagtacattg
    16561 tcgaccacca gggttccacg gctgtgcgag ccgacaaaca ggccgttttt atccttcccg
    16621 tagtcttcca tgatattgcc taaagccaag cctgaattgc tcaagccgat aacggtctta
    16681 ttgcctattt ttgccccttc gagcatttta tgaaaggccg caacggcgat ttcgggcaat
    16741 tttgaaaagc cccgcccatt ggtttccgga ttgtgcagga aataaacatt ttcataggtg
    16801 cgttcatatc ggtttttctc gggattgaaa cgccccacat attgttgggc ggcaaatttg
    16861 gcggctgctt gtatattatt gaaaatgccg ttgacgccga caacgatttt ttcaacggtt
    16921 ttgccggtct ccgggtcggt gtaacgggca gttttcagat ttttccgttc ctggtcggat
    16981 acttcgcgca attcgtagat atttccccca atggctttat aggcttccca ataatcttta
    17041 aatgaggcaa attctttttt caactttact gagttgtcaa actgtttatt tatatcctct
    17101 tcaagctgct tatcttcaat cggctcgccg ttttcatcca ttttaaaggt catcaggcgg
    17161 tgttctgcaa taaactggct gcggtaggct tcgtctgcaa tgccgccgat cagcgtctca
    17221 ttgataaact ctttggcaac atccctattg agttcgactt ctttcagcaa gccttcgcgg
    17281 tctgcttttg ccaatgcctt atgtgcggat gaagtgcctt tttcaatacc ggcaatattt
    17341 ttcctgccct gcgcggaagc aatttgccaa tctccctcac tgatgacgga gcgggtaatg
    17401 gattggcggc tttctgattc tttggcattg cccaacagat tgcccaaacc catttttgcc
    17461 aaggcgtatt tgtcgttgct gtgtatatcg ttttgcttca aaccgaattt caagccgccg
    17521 gcgttgtttt gatttaaatt tgcctcttta aaggtttccc ctttttcaat acgctcactt
    17581 cgatattttt cgttcaactc gtcgtctttc gccgaaacct ggtcaaaacg catcaggctg
    17641 accggtttgc cgccgatttt accgatttct gcttcttttt tggtggaata ctgtccgctg
    17701 gtaggcttcg ggctgtatga aaaaccgccg ctcaagccca aggcggaagc agccgccgaa
    17761 gcatggtttt gaatatcttt atgccagatt tcgcttgttt tcagcaggtt ttttgatttg
    17821 tcggcatctg aaacaacagc ggcgccgacc aatccggttt tgccgtttac gcgaatccga
    17881 tagccgtctc ctcctgcaaa gataccgctt tgctcgttga cggatgcata atccgagctg
    17941 cttttcgagc ggttatagct gccaccgaca ctaaagccgt agcctaccgt aacttgggcg
    18001 gaaacatttt cctgtttgcc tttaaacacg gcggtatcct gcaaactttc gatatgcaga
    18061 ctctctgccg ttacgccaac gcctttgcct ttaagctgcc cgcctttgat gacggtatcg
    18121 ccaccgcttt caatagcggt ttggctgtct ttgctgccga tatggctgtt gcggtaggcg
    18181 gtttcgtcgc cgttgccgta acctttgccg tagtttgctc cggctgtgaa gccgaaactg
    18241 atgcctttgt tgatggcgat ggcgactccg gcattaaagc ctgcggattt gttttcgctg
    18301 cgttcctgat gcgtttggcg ggcggcttca atctgaacgg cattttctgc tttgaggcgt
    18361 gttcctttgc cgccgtacac atcggagccg gtaatcgtga tgcgggagtc tttgcctgcg
    18421 cctgaagcag tcagggaaac tttgccgccg ccggtgattt tgccctcttg cacctgcgtg
    18481 cctttgatgc ggctttcgga ggtgttcttc tgttcgccgt aggtaacgga gacgctgatg
    18541 ccctgaccgg ctgctttttt gggatttcgg gcggcattat agagtgccac gccggaatct
    18601 actcctttat tcaaggcgtt ggcagcagcc atggcattga cccggctgtt tttgcttttg
    18661 ccgacggttt ggactgcttt tacggcgtca accgcgccca ttacggtatt cacaaccgga
    18721 acgctaatgg cgacggttac gcctttttgt tcgtaaacct gcttactctc ttggctgtaa
    18781 cggttttgtg cggcatcgat gctgattttt ccggaggaaa tgccgacatc gccttggggc
    18841 gaggatatgg tcgaaccggt ttgggtgtaa tgttttcctg ccgaaatcag ggtattgccg
    18901 ttcaggctgc cgacgacgct ttctgtgtgg ctgacggtct cggatcggtt ggtttgcgtg
    18961 tcttttttgc tgcccgccgt aaagccgatg ccgccgctgc ccatcagtcc ggatttttct
    19021 tttttgttca tttcggcact gcggctgcgt gtttcggctg ctttaaggac gatattgttt
    19081 tttgccgaga gaatggtatg gttgtctgca atgatattgc tgccagtaac ggtaatatcg
    19141 cgtcctgaaa ccagaatgat ttctttgccg tccagcgtgc cggatacggc ttgtccgttt
    19201 tggttcttga gatggcgggt catcttctgt ttgatgccgc ccccgcttct accggtgtat
    19261 ttcagggcat cttcggtttc ggtatgggct ttgccggctt cgactttgat atcccgtccg
    19321 gctgccagtt tcagacggcc ttgttcgctg ccgacctctg ctgcacggat acggatgtct
    19381 ccttttgcat tcagactgag attgccccgg gtgcggatgg tgctgccgac ttcgttttgt
    19441 tctttgcgaa tcacatagtt gtcggaatca aagatagtgt tctgattgcg ggaaatgccc
    19501 gtcgtatccg agcggatgtc gccgccggca ttcagtacgg tttgaccgtc ttcagattga
    19561 ttggtcaatt cggaagccgt caggacgata ttgttgcctg catccagcaa gacgcttcca
    19621 ttctgcctgc cggtcagata aatgcctgcc acccggccga tattgcgtac cgagccttgc
    19681 tcattctgat tgctgcgggt ctcgctgcgg ctttctatat tgttactcgc tttgagcagc
    19741 agggcgtttt ctgcgccgat gctgccggta ttcgtaatgt cgctgcctgc tgcggcgaag
    19801 atgtttttgc cctgcaaatc accttgcaga tttttaatat tctgtgcgtt taaaattagt
    19861 gcttcgcgcc cggcaattaa gccgccccgg ttttcaatgg cgccgctgcc gatatcaaca
    19921 acgctgccgg acagcaacgc cccttgtccg ttcatatctt tggggcgtgc gcggacatag
    19981 actttgggtt tcaatacggt ttgagttgtc ccgtcgggca gggtaacggt ctcgttttcc
    20041 agccaaacaa tgtcggaagt cagacgggca acctgttcgg cagacagggc aatacccgga
    20101 gtaagctgca attctttggc tatggtaatg ccgttatcca tcaaagcctt gaattgctct
    20161 tcgtcattgg tataaccgtc caagcggcgg tagcctgtca gcttggcgat ttgttcgttt
    20221 accagtttct gctcgtaata gccgtcgccc aaacgcttgt ggatatggtt cgggtcttgt
    20281 tgcagtgcgg caagcatata gccgctgccc agccatttgc ggtagtcggt aaaggcaggg
    20341 tcggtttcaa tcaaatagcc tttgttgttt ggcgcaatgg caaacaagct gctgttcggc
    20401 agagtaaatg taggatggat gccgttttca gcaacaacgg gtacaacagt gccgggtata
    20461 tcagatgcct gttggggcgc atagccttta taggctgaaa tacccatacg gatggagctg
    20521 acttcggggg ccggttcgta gggagaccga ctgtatcccg tagaatcccg ccctttcttg
    20581 tgatgacggt ggtaacggtg caagtcccct ttatcggtta tggtttttgt tcccaaagtt
    20641 tcatcattgt ccaatgcgga ttcgggcgta ccgacgatca atctgccgcc ggcaataatc
    20701 cggctgttat gatttttcag cttggcggca tctaaaacca aattgccgcc actgatgatt
    20761 tgaccgggct tggattcggt aattttgctc tcggcggtga ttcgttcgta atcgtatttg
    20821 taccagacag agtgggggct gccgtccgga gtccgcatat gtaaagactc atcttcaaat
    20881 atttcccagc ccaactcttt ttgagaacct tccggatagc gttctgttct gccttccgcc
    20941 tgatattcga tacggtgttc gcgatgggtt tcttccgtat ggaaacgcag atgctcattg
    21001 gtattctgca aatcttttgt agcgatacgg atattgcctg atgattcgat tgccgcactg
    21061 cggttgtgca gtgatgtatt tgctccttgc acctgtcggc ttccattcaa tgcagaaccg
    21121 atatgaagat cgccggagct ggacaataat gccgcctctc ggttctcaat ttcccgcgct
    21181 ccaatatcca accgctcccg cgctgcaatt accgccgctt tggtttcgcc gttgaccgtt
    21241 tcttcccggt tcaacaaggt atctgcctca actgccacac ggctgccgta aattctgccc
    21301 gtgccggcat tgtccgactt ggcttcggtt tgcagcagcg ttataccgtt gctgttgatt
    21361 aaacccctgt tggtgatgcc gtttttgccg tttaacccgg tgcggtttcc ggattggatt
    21421 ttaccggaga cagtgttgtc gatttgggcg gcggtcaggg atacggtatc gcctgcctgt
    21481 atgatgcggg tgtttttcag acggccttgg gtggagaacg tgagtgtgcg tccggcttca
    21541 atatcgcgtt tgccggcaaa atcatcatga agagaaacgg aaacatcgcg tgcggcggtt
    21601 aatatgccgt cgttgtccag tgattttgcc tgtaaggaaa catctttgcc cgcaataatc
    21661 gtgccgtctg tgttattgat atgcaggctg cttttgcctg tatcgcgaat atccagcaaa
    21721 ccggccgagc cgattaagcc tgcttgatta tttatgccgt ctgaaacctt caatacactg
    21781 cctttgccgc tgcggataaa gccttggcgg ttatcaacgg tttgagcgga aatggtgatt
    21841 tcggcagcat caatcgaacc gctgttgctc aatttgccgt ctgcgcttaa cgtaacgccg
    21901 cctgttgcgg caaaaatccg acccttgttg cggattacgg cgccgttgtc ggtgctgatt
    21961 aaagtgattt tgtctgcgta catcccaccc agtgtggcgg tgtcgatggc aacggtagga
    22021 gtaacagaat ccgaagaaga tggcgcagaa gctgttttgg caagagagcc gtcaaaatcc
    22081 aatttgttct tacccgaaac caccttgaca tctttacccc aaacgcccgc attgatttca
    22141 gcagcacgac taaggatacg ggtgtaatcg gcatcagagg tatccaaacc tttgccccca
    22201 atcacgactt tacccgaaga aacatcaaag cccgtcagat tgccgttatt caaaacagga
    22261 acgcccgaag tcagcgtaac cgaagcggca ttaatcaatc cgccgccatt cacacggatg
    22321 cccgacggat tggcaacgac tacttcggcg cgtttgccgc cgacttcgat ataaccgttc
    22381 aacaacgaag gattactgct gtcaatctgg ttcacaatta cccgcgcttc gccgcgtgcc
    22441 agatggggat tgccttgaat ccatccaccg agttgcgttt gcgtattgct gcggctgttg
    22501 tttagtatta cgcctttttc atcaacatcg aactgcttga atcggttaac agaaacgcct
    22561 tgggatgacg gagtttgaat attgacttgc ggcaaaccgt ttgctgtctg aagaataacg
    22621 gcttgttggt ttttaggggc ggatttgtcg gcaatgatgc cggaagcagg ggcaggggaa
    22681 aacgcagcaa cacccaaagc caacatgaca gaaaaggcag ccatacggaa accgaaggct
    22741 gcccgggcag aagaaacaga agcggcaccg gtcactcgaa ccgaagccgc ctcactatcc
    22801 tgcatactct tgccgtcacg atgaacattc tctgctacag ccatcataca actgcgtttc
    22861 ttgttgaaga taaccttgta gcatcgcttg ttcatgatgg gttttcttaa atgaaatgta
    22921 aggaatagtt aaggacaaaa tataggaaat ttgaattaaa ttgtcaataa aaaactgaca
    22981 gcgtaccctg ataatcagat gttgtacgct aattgtgaga agtatgtcgg gatgattatt
    23041 ttgaagtttt ctttttattc aaaagagtta ctatgaaagt taaccgggcg taaataggct
    23101 tcaataaaca ggttatcgtt atttttcaat tgggaaaaaa tcttccctaa cccatccatt
    23161 cgccaaatta ttatgtggga attcggcaat ttcaataacg gctgttgatc catatctgct
    23221 cggtcattta gcgttataaa gtcgcaaata gcgtcagaat ttacaagatt atcggatttt
    23281 gggaataaat tatgccgtct gaagggcttt cagacggcat aggcggctaa cacgggtgcg
    23341 gcttgcgcca aacggcgcgg caggcgcagg gaatcgcttt ttactttggc gcggtgttcc
    23401 gggtttaatg atgcccgcaa ccgccgtggc tttccctcaa ggcttccgcc gcctgttcgt
    23461 cggcgtggta gctggaacgt accatcgcgc cgatggcggc attgctgaag cccagttcgt
    23521 atgcttcttt ttcaaagatt ttgaactgct cgggcgtaac gtagcgcagg acgggcaggt
    23581 gtccgtctga aggctggagg tactgtccga tggtaatcat ttcgatattg tgcgcccgca
    23641 tatcgcgcat aatttcacgc acgtcttcgt ctgtttcgcc caagccgacc atgatgccgg
    23701 atttggtcgg gatgtgcggc atcatttctt tataacgttt taataagtct aaagaatgtt
    23761 gataattggc accgggacgg gcttttctgt acaggctcgg atgggtttct aggttgtggt
    23821 tcatcacgtc gggcggggtt tcggcaagga ttttgagtgc gatgtccaag cgtcctcgga
    23881 agtcggggac gaggatttcg attttggtgt tcgggctggt ttcgcggatg gctttgatgc
    23941 agtcggcgaa atgctgtgcg ccgccgtcgc gcaggtcgtc gcggtcgacg gaggtgatga
    24001 cgacgtaacg caggttcatg gctttgacgg attcggcgag gtttctcggt tcgtcggggt
    24061 cgagcatatt gggccgaccg tgtcccacgt cgcagaacgg gcagcggcgg gtgcagatgt
    24121 cacccataat catgaaggtc gccgtgcctt tgctgaagca ttcgccgatg ttggggcagg
    24181 aggcttcctc gcaaacggtg tgcatctttt gttcgcgcaa aatgtctttg atttcaaaga
    24241 atttgcgcga tgggagtttg gcgcgtatcc attcgggctt tttcagtttt tcctgaaggg
    24301 ggacgacttt gatggggatg cgcgcggttt tgtccgcgcc tctgagtttg atgccgcgtt
    24361 tggggtcgtc ggttttgatt tcactcattg ttgtctgctt tcggtgtgaa ttgtgtttca
    24421 aggtgtgcgg tgagtttggc ggcgacttcg tccggcgtgg ggcagggttg gacaaaatcc
    24481 gcgatttgcg tcatttccat accggcgtag ccgcaggggt tgatgtgggt aaacgggctt
    24541 aaatccatat tgacgttgag cgcaagcccg tgatagacgg agccgttttt gatacgcagc
    24601 cccagtgagg cgattttgcg ttctccgaca taaacgccgg ggcgtttggg gtctgccgcc
    24661 gcttcgatgc cgtattctgc caatgtggcg atgatgctgt tttcaagcgc ggaaacgatg
    24721 tttctaacac tggttttgcg ccgtttgaaa tcaatcatcg tataaacgac caattgcccg
    24781 ggcccgtgat aggtaatctg cccgccccgg tcgatttgga cgacgggaat gtcgtcgcga
    24841 atcagcaggt gttcgggttt tcccgccagt ccttgtgtga acacgggcgg gtgttcgacg
    24901 acccacagtt cgtcttcggt gtcggcattc cgtccggcat taaaggtttt catcgcttca
    24961 aaagtcggca gatattcgac caaacctttg tgtatgattt tcatctcaaa gtaccacttt
    25021 gaccagttcg tgcgaagtca gcgcacggta gatgttgtcc aattgttctt ggttttcaac
    25081 ctttacctgt acggtggcgc cagtatagtt gcctttgctg ctcggacgcg tggtgatgtg
    25141 gtgcgcctgc gtgtcggggg cgtggaggcg gacggtgtct aaaaccgcct gctcgaactc
    25201 gggatgcacc gcgcccatta ctttcaatgg gaaggtgcag ggaaattcga tgagggatgt
    25261 tttgtttttt tgttcggtca tgatgtgctg ccttgtcgtg tacggtatgc cgtctgaagg
    25321 cgggtttgcc tttcagacgg catcggatgt gcgttatttt agcctaaacc gcgataacag
    25381 gctatcggga aggcggaggc ttttttgacg gcgcggcggt tctgctatac tggcgcacaa
    25441 tattattttt agaagggtgg tttttatgta tcggaggaaa gggcggggca tcaagccgtg
    25501 gatgggtgcc ggtgcggcgt ttgccgcctt ggtctggctg gttttcgcgc tcggcgatac
    25561 tttgactccg tttgcggttg cggcggtgct ggcgtatgta ttggaccctt tggtcgaatg
    25621 gttgcagaaa aagggtttga accgtgcatc cgcttcgatg tctgtgatgg tgttttcctt
    25681 gattttgttg ttggcattat tgttgattat cgtccctatg ctggtcgggc agttcaacaa
    25741 tttggcatcg cgcctgcccc aattaatcgg ttttatgcag aacacgctgc tgccgtggtt
    25801 gaaaaataca atcggcggat atgtggaaat cgatcaggca tctattattg cgtggcttca
    25861 ggcgcatacg ggagagttga gcaacgcgct taaggcgtgg tttcccgttt tgatgaggca
    25921 gggcggcaat attgtcagca gtatcggcaa cctgctgctg cttcccttgc tgctttacta
    25981 tttcctgctg gattggcagc ggtggtcgtg cggcattgcc aaactggttc cgaggcgttt
    26041 tgccggtgct tatacgcgca ttacaggcaa tttgaacgag gtattgggcg aatttttgcg
    26101 cgggcagctt ctggtaatgc tgattatggg cttggtttac ggtttgggat tggtgctggt
    26161 cgggctggat tcggggtttg ccatcggtat gcttgccggt attttggtgt ttgtccctta
    26221 tctcggggcg tttacgggat tgctgcttgc caccgtcgcc gccttgctcc agttcggttc
    26281 gtggaacggc atcctatcgg tttgggcggt ttttgccgta ggacagtttc tcgaaagttt
    26341 tttcattacg ccgaaaatcg tgggagaccg tatcgggctg tcgccgtttt gggttatctt
    26401 ttcgctgatg gcgttcgggc agctgatggg ctttgtcgga atgttggcgg gattgccttt
    26461 ggccgccgta accttggtct tgcttcgcga gggcgtgcag aaat
//
LOCUS       DAWHRA010000010        26444 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWHRA010000010 DAWHRA010000000
VERSION     DAWHRA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.010"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(10..1305)
                     /locus_tag="WIB30_000315"
     CDS             complement(10..1305)
                     /locus_tag="WIB30_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ3796451.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAIELVDERVRKHTIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFRPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1431..2318)
                     /locus_tag="WIB30_000316"
     CDS             complement(1431..2318)
                     /locus_tag="WIB30_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ3796452.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVVYSFNESKLVTVWGG
                     FSTRWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2318..3283)
                     /locus_tag="WIB30_000317"
     CDS             complement(2318..3283)
                     /locus_tag="WIB30_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ3796453.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNMLIKIGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WIB30_000318"
     CDS             complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WIB30_000318"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ3796454.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4618..4725)
                     /locus_tag="WIB30_000319"
                     /pseudo
     CDS             complement(4618..4725)
                     /locus_tag="WIB30_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WIB30_000320"
     CDS             complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WIB30_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ3796455.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WIB30_000321"
     CDS             complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WIB30_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ3796456.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WIB30_000322"
     CDS             complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WIB30_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ3796457.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WIB30_000323"
     CDS             complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WIB30_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ3796458.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8694..9803
                     /locus_tag="WIB30_000324"
     CDS             8694..9803
                     /locus_tag="WIB30_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ3796459.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRYDI"
     gene            10166..11230
                     /locus_tag="WIB30_000325"
     CDS             10166..11230
                     /locus_tag="WIB30_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ3796460.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKITVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            11371..11694
                     /locus_tag="WIB30_000326"
     CDS             11371..11694
                     /locus_tag="WIB30_000326"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ3796461.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            11766..12089
                     /locus_tag="WIB30_000327"
     CDS             11766..12089
                     /locus_tag="WIB30_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ3796462.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            12136..12339
                     /gene="tatA"
                     /locus_tag="WIB30_000328"
     CDS             12136..12339
                     /gene="tatA"
                     /locus_tag="WIB30_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ3796463.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            12343..13029
                     /gene="tatB"
                     /locus_tag="WIB30_000329"
     CDS             12343..13029
                     /gene="tatB"
                     /locus_tag="WIB30_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ3796464.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDFRPKHR
                     AKPKLRVRKS"
     gene            13043..13813
                     /gene="tatC"
                     /locus_tag="WIB30_000330"
     CDS             13043..13813
                     /gene="tatC"
                     /locus_tag="WIB30_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ3796465.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            13866..14471
                     /gene="maf"
                     /locus_tag="WIB30_000331"
     CDS             13866..14471
                     /gene="maf"
                     /locus_tag="WIB30_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ3796466.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALPLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            14487..14840
                     /locus_tag="WIB30_000332"
     CDS             14487..14840
                     /locus_tag="WIB30_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ3796467.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            14830..16644
                     /locus_tag="WIB30_000333"
     CDS             14830..16644
                     /locus_tag="WIB30_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ3796468.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            16834..17511
                     /locus_tag="WIB30_000334"
     CDS             16834..17511
                     /locus_tag="WIB30_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ3796469.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            17525..18931
                     /locus_tag="WIB30_000335"
     CDS             17525..18931
                     /locus_tag="WIB30_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ3796470.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            19049..21475
                     /locus_tag="WIB30_000336"
     CDS             19049..21475
                     /locus_tag="WIB30_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ3796471.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDNLSTAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISLITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HPKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLGPEARYTRFMTHTNELPSATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRIMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            21520..21765
                     /gene="rpsP"
                     /locus_tag="WIB30_000337"
     CDS             21520..21765
                     /gene="rpsP"
                     /locus_tag="WIB30_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ3796472.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            21781..22290
                     /gene="rimM"
                     /locus_tag="WIB30_000338"
     CDS             21781..22290
                     /gene="rimM"
                     /locus_tag="WIB30_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ3796473.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            22290..23039
                     /gene="trmD"
                     /locus_tag="WIB30_000339"
     CDS             22290..23039
                     /gene="trmD"
                     /locus_tag="WIB30_000339"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ3796474.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKTQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            23055..23420
                     /gene="rplS"
                     /locus_tag="WIB30_000340"
     CDS             23055..23420
                     /gene="rplS"
                     /locus_tag="WIB30_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ3796475.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            23738..24463
                     /locus_tag="WIB30_000341"
     CDS             23738..24463
                     /locus_tag="WIB30_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ3796476.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMQRFADRQIAYLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            24499..25374
                     /locus_tag="WIB30_000342"
     CDS             24499..25374
                     /locus_tag="WIB30_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ3796477.1"
                     /translation="MNPYDLLITPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            25451..26371
                     /locus_tag="WIB30_000343"
     CDS             25451..26371
                     /locus_tag="WIB30_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ3796478.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHDHEGHHHNHGEFDPHVWNDPVLMSTYAQNVAEALIKA
                     DPEGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEF
                     IAPQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKL
                     YSDALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         6852 a         7907 c         6081 g         5604 t
ORIGIN      
        1 ctgcgacgct taacggctgt ctttccactg ataatatttc gagccgagga aagaaccgag
       61 tttgcgcaaa aacggttgca ggataatatt cggctggcgc aaccgctcaa acggcttcag
      121 acggcattcg tcccctaaaa ttgcttcggc aaccgccaga cctgcaatgc ctgttatcgc
      181 catcccgtgt ccggaataac cttgcgtata aaaaacattc ggggctaaac gtccgaaatg
      241 cgggacaagg ttggcggtaa tgtcgcactc cccgccccac gaatattcga ttttgacatc
      301 ggcaagctgc ggaaaaactt taagcatatc ttggcggaca agctcggtca tacgcgcggg
      361 attgtcgata aactcgttat ccttaccgcc gaaaagcagt ctgccgtccg cgctgaggcg
      421 gtaataatcc aaaatatggc ggttgtcgca tactgccata ttgttgcgga taagcccttt
      481 tgcacgcgcg cccaagggtt ctgtggcaat aataaaggtg ctgacagcaa tcgccttgcg
      541 ttccaaaggc cggaatatcg gattcaaacc ggcataagta ttgacggcgt acaccagatt
      601 tttgcattcg acgctgcctt cgggggtgta aaccagccaa ccgttttgat aaggttcaat
      661 gcgtatcatg ggagaatgct caaaaatctt cgtaccagct tcggcagcgg cgcgggcgat
      721 gcccaacgtg taattgagcg gatggagatg cccggacaag ggatcgaact gtgcgccttg
      781 gtacatatcg ctgtcaagct gctgcttcag ttcagtgtta tcccagagtt gataatgact
      841 cgcaccgtaa tgccgttggg cgtgttcatg ccattgttgc aattcttccc aatgctgcgg
      901 acggacggca accgtggcat aaccgcgctg ccaatcgcaa tcgatggtat gtttgcggac
      961 gcgttcgtcc accagttcga tcgcctgcaa agactgttgc caaaaccatt gcgcctgttc
     1021 caaaccgacc tgtttttcaa tttcctccat accgcaggcg taatcgctga taacctgccc
     1081 gccactccgt cccgacgcgc cgaaaccgat acgcgcggct tccaacacaa ccgtttcatg
     1141 tccctgctcc gccaagggca atgcagtgca caaaccactc aatccgcctc cgatgataca
     1201 ggtatcggtt ttcagacggc attgaagttt cggataaaca gtatgaggat taaccgaact
     1261 gaaataataa gaaggcagat attcttgaaa atcagggcga atcattgtgt ttgctttatc
     1321 gggtatattt tcggacggaa tgatacaggc tgtcgagcca tatcgtccaa acagaaaatc
     1381 ggttgaagaa aacaggctga cccagtcatg cggtcagcct gccttattaa ttaatttgct
     1441 ttctcggcag ccaatttttc ttggcggtag gcttctgccg cttctcggtc acgcttggtt
     1501 gcctgcctca tcatccaata attgacgatg atgaccaatg ttccgatgat gccgattagg
     1561 atggttgcca aaacgttcat ctgcggatcg agacccaact tgattttgga gaaaatcacc
     1621 tgcggcaatg tggatgaacc ggggccggag aggaatgagg taatcaccaa atcatccaaa
     1681 gacagggtaa tgccgagcag aaagcctgaa gcgatggcag gggcaatcaa aggcaaagtg
     1741 atgacaaaaa agattttcag cggacgcgcg cccaaatcca ttgcggcttc ttcgagcgat
     1801 tggtcaagct caaccagacg cgaacggata acaacggtaa tgtatgccat gcacagcgtc
     1861 gtatgtccta agaaaatagt gaaaaagcca cgatcgaagt agagatgttg taaccattcg
     1921 ctgccctgca aaaatatctg cacctgaata atcagcagca gcatagacag gccggtaatc
     1981 acgtcgggca tcaccatagg cgcggaaatc ataccggcaa acagcgtgct gccgcgaaaa
     2041 cgtttgatac gcgccatcgc atagcccgcc agcgtgccca aaacgacagc ggcaagcgaa
     2101 gacacgacgg caatacgcag cgacagccaa gcagcttcca agatggtgtc gttttccaac
     2161 aatgcgccgt accacctggt cgaaaagccg ccccaaacgg taacgagctt ggattcgtta
     2221 aacgaataga caaccaaaac gaccagtggg atatacagaa acgccagcga caatgccaac
     2281 atcagtttca agaaccaaga caatttggat ttctgcatta tttggctcct tcttccaatt
     2341 cgcggttttc ataatgctga aacagggcaa tcggcacgac cagcagcgcg accatcacga
     2401 cggcgacggc ggaagccagc ggccagttgt tttgatcgaa gaacgcctgc cacaagactt
     2461 taccaatcat caggttttcc gaaccgccga ccagctcggg aatgacgaac tcgccgacag
     2521 cagggacgaa aaccaacatc gaacctgcaa taatgccggt tttagacaga ggcagtgtaa
     2581 tcgtcaagaa cgacttgacc ggtcccgcac ccaaatcgga agctgcttcg agcaggcggt
     2641 tgtcgagttt caccagctgc gtgtaaagcg gcagaatcat aaacggcaga taggcataaa
     2701 ccatcaccag gtttaacgaa aacgcattgt agaacaaatc taatggctcg ctgataatac
     2761 ctattttaat caacatattg ttgacgatgc cgttgtgccc gagcaggccc atccacgcat
     2821 agacgcgcaa taggaaagat gtccaaaagg gcagcataat ggcaagcagc aaaccattgc
     2881 ggacagaagg attggcacga gaaatcgcat aggcggtcgg ataaccgacc aacaggcaaa
     2941 tcactgtcgt catcaatgcg gttttgattg aagaccagta ggtcatcaga tagatattgc
     3001 tgttttcact gtcgccgaac gggttaaggg tatgccaaaa attttggaaa atatcggcat
     3061 agttttgata actgatggcg atattcagac gacctaaatc ttcgtcgatg ctggtcagcg
     3121 gcgtaaacgg cggaatggcg atttcttgtt cggcaaagct gattttcagc acgatggcga
     3181 acggaatcag aaacagcacc aaaagccaaa tatacggtac ggcaatcacc gcacgctgcc
     3241 ccggacggcg gaacagtttg tttttcagtt tattaaggtt cattgcattc cccttaaatc
     3301 aacggaacag tggcgtaggc tggttttccg gccagctgat atagacggtt tcgtcccaag
     3361 ttggcggtgt aatgttgcgc acataccagt aaggggcggg gacttggctc ttgacgacgc
     3421 gcccgttggc gagtttgata tggtatatgg cgaagctgcc caaataggcg atttctttta
     3481 ccgtgccttt cgcccagttg tagtcgccca aatattcggg tttttcttta tataaatcaa
     3541 tatcctctgg tcgaatacta acccaaaggt cctgctcgct cggaccaccc aaaccgtggt
     3601 cgatgcggac gtggttttcc aaaccttcgc attcgataac ggcatagtcg gcatgatctt
     3661 caatcaccac accgtcaaaa atattggttt cgccgataaa ctcggcagtg aagcggctgt
     3721 tgggatagtc gtacacgtcg ctgggtgtgc cgacttgctg caactgaccg tcggacataa
     3781 tggcgatgcg ggtcgccatc gtcatcgcct cttcttggtc gtgtgtaacc ataatacagg
     3841 ttacgccgac ttgttccagc gtattgacca actcaagctg ggtttgttgg cgcagttttt
     3901 tgtccaacgc gccgaggggc tcatccagca gtagaatttt cggacgtttt gccagactgc
     3961 gtgccaaagc aatgcgctgt tgctgaccgc cggacaattg gtgcggtttg cgtttagcaa
     4021 atttagtcat ctgaaccagg cggagcattt cttcgacgcg cgcggcgatt tcgcctttag
     4081 gcattttgtc ctgtttcaga ccgaaggcaa tgttttgttc tacggtcata tgcggaaaaa
     4141 gcgcgtaact ttggaacatc atattgatgg ggcgatcata gggtgcaagt ttggtaatat
     4201 cctgaccatc aaggataatt tttccctgat tgggactttc catacccgcc agcatacgca
     4261 gcagcgtgga ttttccgctg ccggaactgc ccaaaagagc gaaaatttcg tgttgataaa
     4321 tgtccaagtc gatgttatcg acagcgtaat tgtcaccaaa ctttttcacc aaaccttgga
     4381 tttgcagata aggcttggtt gaagacgcag tggttgcggt cataatggca atactccaat
     4441 aaaaagacga gtaccggcaa aacggatgtt cgaatgggtg ataaaaagct gtttgattgc
     4501 tggcgggagt taaacgtttg atgccgtctg aaactcttgt aaagcgcacg ggcagcatga
     4561 aatggaacaa gattccaaag aactttatat tatattagtt tatgcggttt tcgggcaata
     4621 tagtggatta aatttaaacc agtacagcgt tgccttgcct tgccgtacta tttgtactgt
     4681 ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctataaatat ggatttgagc
     4741 ttgtcggaaa gcaacagaaa agaaaacacc gcccattttt ctgggcggtg tcggaaagcg
     4801 taattattta cgcagaccca agcgggtaat caacgcgcga tacgtatcgg gctgggtacg
     4861 gcgcaagtag gccagcaggc ggcggcgttg gctgaccatt ttcaacaggc cgcgacggct
     4921 gtggtggtct ttggggttgg ctttgaagtg gggggtcagg tcgttgatgc ggaaagtcaa
     4981 cagagcgact tgtacttcgg aagagccggt gtcgccttct ttgcgttgga aatctttaac
     5041 gatttgtgct ttttgttcta cggtcagtgc cataatgaaa actccaaaaa tataagaatc
     5101 cccataggga ttcagacaag tttgccaagc ctgaagacaa cggcaaactc cctatgccca
     5161 agataggaca acgtggcatt atgacacaat tcccgcattt ctgcacaata ttttaagacc
     5221 gtggaagtaa ccgttaaaaa tgccgtctga agcattgtct gcttgagacg gcaatgttca
     5281 gacggcatat gcgcttcaaa ccacgatttc ttctttcttc ttcggttctt taccgatatt
     5341 gtcgcggctc aaaccgaaca ttagcagaag cgggctggca accaatacgg aagaataaat
     5401 gccgaacacg atgccaatgg tcaacgccat agaaaagccg tgcaaggccg caccgccgaa
     5461 caccagcatg gatacgacca tcgcctcggt cgaaccgtgg gtaatgatgg tgcggctcat
     5521 cgttgcggta atcgcgttgt cgatgacttc cggcacagca tgtccgcgca tcgccggctt
     5581 gcggaagttt tcacggatac ggtcgaagac gacgacggat tcgttcacag aatagcccaa
     5641 tacggcaagg atacccgcca agacggtcag cgaaaattcc cattggaaga aggcaaagca
     5701 gccgagaata atcacgatgt cgtgcatatt ggcgataatg gcagatacgg caaaacgcca
     5761 ttcaaaacgc atcgacaggt aaataatgat gccgataacg acaaaaccta aagccatcaa
     5821 tccattactt accaattcct caccgacttg cgggccgata aattcgactt ggcgcaaggt
     5881 aacgtcggga ctgtcttttt tcagcaaatc cataacctga ttggacaact gtgcggaagt
     5941 aacaccttct ttgttcggca ggcggatcat gatgtgtttg ttcgtaccca atgcctgaac
     6001 ctgtacatca cctattttca gcgtatcgag gcgttcgcgc atcttattga catccgcacc
     6061 ctgctgatat tggacttcca ttaccgtacc gccggtaaat tcgacagaga aattcagacc
     6121 tctggtaacc aaaaagaata cggcggcaat aaaggtaacc aacgaaataa aggtcgtcag
     6181 tttgccgtag ctcataaacg gaatatcgcg tttgatttta aagagttcca tagcttactc
     6241 cttacctgcc gcagtttcgg cttcaggttt ccataccgca ccgatggaaa tattctgcaa
     6301 tttgcgtctg cgtccgtacc acagattgac caacgcacgg aatacgacga cggaggaata
     6361 catcgaagtc agaataccca aacagtgtac gaccgcaaaa ccgcgtaccg ggccggaacc
     6421 gaataccaaa agcgcgatac cggcaatcag cgaagtcagg ttggaatcga caatggtcgc
     6481 ccatgcgtgt tggaaaccga gattgattgc ctgctgcggc ggcacgccgg cacgcaattc
     6541 ttcgcggata cgttcgttaa tcaagacgtt ggagtcgatt gccataccca aagtcaacgc
     6601 cagcgcggcc atacccggta acgtcaacgt tgcctgcatg gcagacaaaa taccgattag
     6661 gaacagtatg ttggcactca atgcaatggt agaaaagaaa cccatcagac gatagtaaac
     6721 caccatgaat gcagcaacga tggcaaaacc ccataaagtc gaatggaagc ctttttcgat
     6781 gttctcctta cccaaagacg gaccgatggt acgttcttcg acaatctgca tcggcgcggc
     6841 aagagaaccg gcacgcaaca gcaaagacgt atcattggct tcggctgtcg tcatgcttcc
     6901 ggaaatttcc acgcgtccgc cggtaatggc agtacggata accggcgcgg ttacaacctc
     6961 ggattttcct tggtcgatca aaaccatcgc catgcgtttg ccgacatttg cggcagtcag
     7021 ttcgccaaaa atgctgccac ccgcgctgtc caagctcaga ctgacggcag gtgcgcccat
     7081 ttggtcgaaa ctcggttgcg catcgttgat gttgtcgccc gtcagctcga cctgtttgct
     7141 gattagcaga gtttcgggac gatctccgcc gcttgaaagc agctcataac cgctcggcac
     7201 gttgccttcc aatgcctcgc gcaacttggc aggatcgtcc tccaccatac gcaattccaa
     7261 agtcgcggta cggccgatga tgtcttttgc cttggcagta tcctgaacgc ccggaagctg
     7321 cacgacgata cggtcggcac cggactgctg gatgacgggt tcggccacgc ccaactcgtt
     7381 cacacggttg tgcagggtag tgatgttctg tttgaccgca tcggaacgca ctttattgac
     7441 cgcctcttcc gaaagtgtca agacgatatt gctgccgtct gaattcagcg ttgcttcagg
     7501 aaacagcttg cgtaactgcg gcagtgcctt ttgcacatca cctgcatcct gcaaagggac
     7561 ggtcaggctg tttccagcct gacgcaccgt gccgctgcgg attttttcgc ggcgcagttc
     7621 gcggcggatg tcgcccgaat aacgttcaaa cgttttctgc atcgccgctt tcatgtccac
     7681 ctgcatggtg aaatgcacac cgccgcgcaa gtccagaccc aaaaacatcg gattggcttt
     7741 gattttcgcc atccactcag gactgtctgc caaaaggttg agtgcggtaa tataaccttc
     7801 gcccaaagta ttttcgatga cgtcgcgcgc tttaagctgc gtttcggtgt ctttgaaacg
     7861 cactttcagt gaattgtcca caacaaacat cccgtcggtc tgaatacccg cgttttgcag
     7921 cgcggcatcc actttggatt gagtctgttc gttgatgatg atggcttgtc ggttggtcga
     7981 tacctgcacg gcgggtgttt cgccgaatag gttgggcagc gaatacactg cggcaaccgc
     8041 aatcgtgaac acaatcagca gatatttcca taaaggataa cggttcatca ttgttcctta
     8101 atggttggaa ccccaccctt tcggtggtgt cggaatcggg ctatttcaga agaggcaaaa
     8161 acccttccca gccaggcaag accggaaagc ggcatcctga atatgccgcc ctgcgtgtcg
     8221 gaacatggtc aagccttcgg ttggaattca aaacaaagtg ccgcattcgg gcttttccga
     8281 tgcggcttgt cggcacaaat caatcgactt ttgcggcaat cgcattgcgt tctacttcga
     8341 cctcgatttt tgtaccctgt ccgatatcca cggtaaaaaa ctgttcgccg actctggtta
     8401 ccttaccctt gaaacctgcc gccaagacca ctttgtcgcc gactttcaag gcggcaagca
     8461 ttgcctgatg cgctttgaat ttcttttgct gcggacgcat gatcaggaag tagaacacca
     8521 ccataatcaa cactaaagga gcaaattgtg caacagcttg attcataatt tatccgttct
     8581 ttctaatatg gttgaaaatc gagaggggta tataataaca taagaccgta aacaatatat
     8641 cgggtttgcc gtccgtaccg accgtatacc gcagcctgcc cgtccacaaa cccatgtcct
     8701 tgacccgcct aatcttgaaa ttctatgcac tgttgcgcct ttttttgggc aaaaacgccc
     8761 gcaccgcatg gatttcgcat cccgcctgtg ccgggcacga acccggcgca aaccatcccg
     8821 attcgcccga ccgcatcctc tgcatcgagc aggcattgcg ccgcgccggt atttggcagc
     8881 acctccaaac catagaggcg gaagaaatca gcgatacgcg cctcgcactt gtccactcga
     8941 gcaaatatct gaaccgtttg gaatcttgcc tgccccaaaa aggcaagatt tcccgcctgg
     9001 ataacgacac tgcaatcagc acaggctcgc tgtctgccgc acgctttgcc gccggttcgg
     9061 cagttcaggc agtcgacatg gtcatgaacc gtaaagcatg gcatgccttt tgcgccgccc
     9121 gcccgcccgg acaccatgcg ggcagcggca aagccggcgg attctgcctg ctgaacaacg
     9181 ttgccgccgg cgtcatgcat gccattgccg aataccgcct gaaacgcatt gccgtcatcg
     9241 atttcgatgt ccactacggc gacggtacgg cagaaatatt caaagacgat ccgcgcatcc
     9301 tgtttttcaa cctatttgaa accgaccttt tccccttccc cgaaaacaac gatatgcccg
     9361 acggcggcaa tatggtgcac ctgcccttgc cgccgggaac gggcagccgc acattccgcg
     9421 aagccgtccg caggcagtgg ctgccccgac ttgccgcatt caaacccgaa ctggtgctgc
     9481 tgtcggcagg attcgacgca caccgtctag acgaatcggg caggctcaac ctgcacgagg
     9541 cggattttgc ctggctgaca cacaaaatca ttcagacggc atcgggctgc cccggcaaaa
     9601 tcgtatccgt actcgaaggc ggctacaccc ttgaacctct ggcacaatct gctgccgagc
     9661 atatccgcgt gctggcaggg ctgggcaaat ccgatgccgc aaccgcctat cagaaaacac
     9721 tggacccaac aaaaaaacgg tttgccaaac cgaaaaccgg acaggtgcgc caacctaccc
     9781 aatccgaccg ttacgacata tagtggatta acaaaaatca ggacaaggcg acgaagccgc
     9841 agacagtaca aatagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt
     9901 ctctttgagc taaggcgagg caacgccgta ctggtttttg ttaatccact ataaatgccg
     9961 tctgaagccg attcggtttt cagacggcat cattatttga cctgacgcaa acagaaagcc
    10021 ttccgcgtgc ttaaacacat ctcttgtagt aaaatatcta aacagattca actggaaacg
    10081 gtcaggcttc ttcacgccat tcccatttca accgccgcct gccagattca gacggtattc
    10141 ctacttgaaa acaaggagcg ttacaatgaa agcagcacgt ttttacgata aaggcgacat
    10201 ccgcatcgaa gacatccccg aaccgaccgt cgcccccggc actgtcggca tcaatgtcgc
    10261 ctggtgcggc atctgcggta ctgacctgca cgaattcatg gaaggcccga ttttcattcc
    10321 gccttgcggt catccgcacc cgatttccgg cgagtccgca cccgtcacga tgggacacga
    10381 gttctccggc gtggtctatg ccgtcggcga aggcgtggac gacatcaaag tcggccaaca
    10441 cgtcgtggtc gaaccctaca tcatccgcga tgacgtaccg accggagaag gcagcaacta
    10501 ccacctctcc aaagatatga actttatcgg cttgggcggc tgcggcggcg gtctgtccga
    10561 aaaaatcacc gtcaaacgtc gttgggtgca tccgatttcc gacaaaatcc cgttggatca
    10621 agccgctttg atcgaaccgc tgtctgtcgg acaccacgcc tatgtacgca gcggcgcgaa
    10681 agcaggcgac gtcgcattgg tcggcggtgc aggtccgatc ggtttgctgt tggctgccgt
    10741 gttgaaagcc aaaggcatca aagtcatcat caccgagttg agtaaagcac gcaaagacaa
    10801 agcgcgcgaa tccggcgttg ccgactacat cctcgacccg tccgaagtcg atgttgtcga
    10861 agaggtgaaa aaactgacca acggcgaagg cgtggacgtg gcattcgaat gtaccagcgt
    10921 gaacaaagtg ttggatactt tggtcgaagc ctgcaaacct gccgccaatt tggttatcgt
    10981 atccatctgg agccaccctg caaccgtcaa cgtccacagc gtcgtgatga aagagctgga
    11041 tgtgcgcggc acgattgcct actgtaacga ccacgccgaa accatcaaac tggtcgaaga
    11101 aggcaaaatc aacctcgaac ccttcatcac ccagcgcatc aagctggacg aactggtttc
    11161 cgaaggcttc gagcgtctga tccacaacaa cgaatccgcc gttaaaatta ttgtgagtcc
    11221 aaacctgtaa gcaaattaca tttgcaacag cacaaatgcc gtctgaaacg ctcagacggc
    11281 attttccaac gaagcgcaac aggcgtattg cccgagagca gccgtttagc ctatcattcc
    11341 gaacaaacag aaacaaacag aaggaaaatc atgggagatt ccgtactatc cgccatccaa
    11401 caaaccatca cccagcgaaa atctgccaat ccgtccgaat cttacgtcgc acagctcttg
    11461 cataagggcg aagacaaaat cctaaaaaaa gtgattgaag aggcgggcga agtgttgatg
    11521 gcatccaaag acaaaaaccc gtcccacctg gtttacgaag ttgccgactt atggtttcac
    11581 accatgattc ttctgacaca ccacgacctg aaggcggaag acgtattgga cgaacttgcg
    11641 cgccgccaag gtttgtcggg cttggccgaa aaagccgctc gcacagaatc ttgaatttat
    11701 attaaaatcc gcactttccc acattcaatc cgtctgaccg cttttcagac ggcatcggag
    11761 ccgttatgga caactgtatt ttctgcaaaa tcgccgccaa agacattccg gcgcaaaccg
    11821 tctatgaaga cggcgaaatg gtttgtttca aagacatcaa ccccgctgct ccggttcatc
    11881 tgctgctgat tcccaaagtc catttcgatt cgttggcaca cgccgcgccc gaacatcagc
    11941 cccttttggg aaaaatgatg ctgaaagttc ccgaaatcgc caaagcggca ggactggcag
    12001 acggcttcaa aaccctgatc aacaccggca aaggcggcgg acaagaggtt ttccacctgc
    12061 atatacacat catgggcaca cccgtataaa ccgttatttc acaatcaacc cctaatactt
    12121 acttaaggat acatcatggg cagtttttct ctgacgcact ggattatcgt actgattatc
    12181 gtcgttttga tattcggcac caaaaaactg cgcaacgtcg gcaaagacct cggcggtgcg
    12241 gttcatgact tcaaacaggg gctgaacgaa ggtacagacg gcaaagaagc ccaaaaagac
    12301 gatgtaatcg aacacaaaaa agacgaagac aaagcgtaat ttatgtttga tttcggtttg
    12361 ggcgagctgg tttttgtcgg cattatcgcc ctgattgtcc tcggccccga acgcctgccc
    12421 gaggccgccc gcaccgccgg acggctcatc ggcaggctgc aacgctttgt cggcagcgtc
    12481 aaacaggaat ttgacactca aatcgaactg gaagaactga ggaaggcaaa gcaggaattt
    12541 gaagctgccg ccgctcaggt tcgagacagc ctcaaagaaa ccggtacgga tatggaaggc
    12601 aatctgcacg acatttccga cggtctgaag ccttgggaaa aactgcccga acagcggaca
    12661 cctgccgatt tcggtgtcga tgaaaacggc aatccgcttc ccgatgcggc aaacacccta
    12721 tcagacggca tttccgacgt tatgccgtcc gaacgttcct acgcttccgc cgaaaccctt
    12781 ggggacagcg ggcaaaccgg cagtacagcc gaacccgcgg aaaccgacca agaccgcgca
    12841 tggcgggaat acctgactgc ttctgccgcc gcacccgtcg tacagaccgt cgaagtcagc
    12901 tatatcgata ccgctgttga aacccctgtt ccgcatacca cttccctgcg caaacaggca
    12961 ataagccgca aacgcgattt tcgtccgaaa caccgcgcca aacctaaatt gcgcgtccgt
    13021 aaatcataaa gagagcaatc cggtgtccga aacacaaaac gaacaacccg tccaaccgct
    13081 tgtcgagcat ctcatcgagc tgcgccgccg cctgatgtgg acggttgtcg gcatcttagt
    13141 ctgctttttc ggcctaatgc cgtttgccca acaactctat acttttatcg ccgacccgct
    13201 gatggcaaac ctgcccaaag acaccagcat gattgccaca gatgtcatcg caccgttttt
    13261 cgttccggtc aaagttaccc tgatggcggc atttttaatt tcgctgccgc atacgctcta
    13321 ccaaatctgg gcatttgtcg cccccgcact ctaccaaaac gaaaaacgcc tgattacgcc
    13381 gctcgtcctc tccagcgtca gcctgttttt catcggtatg gcatttgcct actttttggt
    13441 tttccccgtc attttcaaat tccttgccgg cgttacccct gtcggggtca atatggcgac
    13501 agacatcgac aaatacctct ccttcatctt ggggatgttt gtcgcattcg gtacaacgtt
    13561 tgaagtcccc attgtcgtta tcctgttaac caaaattggt gtggtaacaa ccggacagct
    13621 caaacgcgcc cgcccctatg tgattgtcgg cgcgtttgtc attgccgcca tcatcacgcc
    13681 gcccgatgtg atttcacaaa ccctgcttgc cattccgctg attctcttat acgaagcagg
    13741 tatttggttc ggacgctttt tcacgccacg ttcagaacag gatggcgaca tacagccgcc
    13801 tgcaacaacc tgacactatg ccgtccgaac ctccgcctca taccgccaca gattaaggaa
    13861 tacctttgaa taccctctat ttaggttcaa acagcccgcg ccgaatggaa atcctgacgc
    13921 agttgggcta ccgcgtcatc caactgcccg ccggcatcga cgaatccgtt aaagccggcg
    13981 aaacaccttt cgcttacgtt caaaggatgg cagaagaaaa aaaccgaacc gccctgcccc
    14041 tcttttgcga aaccaacggc acaatgcccg attttcccct gattaccgcc gacacctgcg
    14101 tcgtttcaga cggcatcata ttaggcaaac cccgctccca agccgaagca atcgaatttt
    14161 taaaccgatt gtccggcaaa caacataccg tcctgactgc tgtctgcatt cattatcgcg
    14221 gcaaaacgtc aagccgcgtc caaaccaacc gcgtcgtttt caagcccctg agttcggaag
    14281 aaatttccgc ctatgtgcaa agcggcgaac cgatggacaa agccggcgcc tacgccgtac
    14341 aaggcatagg cggcatcttt atccaatcta tcgaaggcag cttcagcggc attatggggc
    14401 tgcccgttta tgaaaccgtt tcgatgttgc aggatttggg ataccgtacc ccacctttca
    14461 tccgagccta acccgtaaag accgccgtga acagacaaac cgcttacctc cttacctctt
    14521 tcagcctgat cgcactgata atcctgtccc tttcctggga actgtggatt gcaccgttgc
    14581 gcccgggcgg ctcgtggctc gcgctcaaag ccctccccct ctgtctgccg ctttcaggca
    14641 tcttgaaaaa gaaaatctat acctaccaat acagctccat gctggttctg atttactttg
    14701 ccgaagccgt catgcgcctg ttcgacgcct atcccgcaga acagatttgc gccgcgcttt
    14761 ccgcagtatt cagcatcatc ttcttcatat cctgcctgtc gttcgtcaaa caatacaagg
    14821 aaacaaacaa tgcccgctga aacgaccgta tccggcgcgc accccgccgc caaactgccg
    14881 atttacatcc tgccctgctt cctttggata ggcatcgtcc cctttacctt cgcgctcaaa
    14941 ctgaaaccgt cgcccgactt ttaccacgat gccgccgccg cagccggact gattgtcctg
    15001 ttgttcctca cggcaggaaa aaaactgttt gatgtcaaaa tccccgccat cagcttcctt
    15061 ctgtttgcaa tggcggcgtt ttggtatctt caggcacgcc tgatgaacct gatttacccc
    15121 ggtatgaacg acatcgtctc ttggattttc atcttgctcg ccgtcagcgc gtgggcctgc
    15181 cggagcttgg tcgcacactt cggacaagaa cgcatcgtta ccctgtttgc ctggtcgctg
    15241 cttatcggct ccctgcttca atcctgcatc gtcgtcatcc agtttgccgg ctgggaagac
    15301 acccctctgt ttcaaaacat cattgtttac agcgggcaag gcgtaatcgg acacatcgga
    15361 cagcgcaaca acctcggaca ctacctcatg tggggcatac tcgccgccgc ctacctcaac
    15421 ggacaacgaa aaatcccgcc cgccttgggt gcaatctgcc tgattatgca gaccgccgtt
    15481 ttaggtttgg tcaattcgcg caccatcttg acctacatag ccgccatcgc cctcatcctt
    15541 cccttctggt atttccgttc ggacaaatcc aacaggcgga cgatgctcgg catagccgca
    15601 gccgtattcc ttaccgcgct gttccaattt tccatgaaca ccattctgga aacctttacc
    15661 ggcatccgct acgaaactgc cgtcgaacgc gttgccaacg gcggtttcac agacttgccg
    15721 cgccaaatcg aatggaataa agcccttgcc gccttccagt ccgccccgat attcgggcac
    15781 ggctggaaca gttttgccca acaaaccttc ctcatcaatg ccgaacagca caacatatac
    15841 gacaacctcc tcagcaactt gttcacccat tcccacaaca tcgtcctcca actccttgca
    15901 gagatgggaa tcagcggcac gcttctggtt gccgcaaccc tgctgacggg cattgccggg
    15961 ctgcttaaac gccccctgac ccccgcatcg cttttcctaa tctgcacgct tgccgtcagt
    16021 atgtgccaca gtatgctcga atatccttta tggtatgtct atttcctcat ccccttcgga
    16081 ctgatgctct tcctgtcccc cgcagaggct tcagacggca tcgccttcaa aaaagccgcc
    16141 aatctcggca tactgaccgc ctccgccgcc atattcgcag gattgctgca cttggactgg
    16201 acatacaccc ggctggttaa cgccttttcc cccgccactg acgacagtgc caaaaccctc
    16261 aaccggaaaa tcaacgagtt gcgctatatt tccgcaaaca gtccgatgct gtccttttat
    16321 gccgacttct ccctcgtaaa cttcgcccta ccggaatacc ccgaaaccca gacttgggcg
    16381 gaagaagcaa ccctcaaatc actaaaatac cgcccccact ccgccaccta ccgcatcgcc
    16441 ctctacctga tgcggcaagg caaagttgca gaagcaaaac aatggatgcg ggcgacacag
    16501 tcctattacc cctacctgat gccccgatac gccgacgaaa tccgcaaact gcccgtatgg
    16561 gcgccgctgc tacccgaact gctcaaagac tgcaaagcct tcgccgccgc gcccggtcat
    16621 ccggaagcaa aaccctgcaa atgacccgcg ccggcggatg cggataccgc ccgaaatgta
    16681 aagctccatg caagacattg caaaaaacag caaaccggta gggaaatacg ctatcaaaaa
    16741 acattgcagc cgtgttaaga taaaccgtca aacaatcttt tcacgccccg cccgaaacag
    16801 ggtcggggca tacccttacg aaaaggaaac accatgagcc gcgtattact cgtagatgac
    16861 gatgccctgc taaccgaact gctgaccgaa tacctgagcg ccgaaggtct gaacgtccgc
    16921 agcgttcccg acggggaagc aggcgtacag gaaatcctga gcgggcagta cgatgtagtc
    16981 gtattggatt ccatgatgcc caaaatgaac ggcttggatg tcttgaaaaa cgtacgcgcc
    17041 cgaagcaccg tccccatcat catgctgacc gccaaaggcg acgacatcga ccgaatcatc
    17101 ggcttggaaa tgggcgcgga cgactatgtc cccaaaccct gcacaccacg cgaactcttg
    17161 gcacgcatca atgccatcct gcgccgcgca caacacagcg gcgaacagaa caacgcaccc
    17221 aacagcatct ccgtcagcga tgtcgtcctg taccccgcca aacgccaggc atccgtcaaa
    17281 gacatgccgc tcgaactgac cagcaccgaa ttcaacctgc tcgaagtcct gatgcgccat
    17341 gccggacagg tagtcagcaa agaaaccctg tccgtcgaag cactcgaccg caagctggca
    17401 aaattcgacc gcagtatcga cgtacacatc tccagcatcc gccacaagtt gggcgatgcc
    17461 tctctgattc aaaccgtacg cggcttgggc tacctgtttg tcaaaaactg aaataaacag
    17521 ataaatgaaa ctgttccaac gcatcttcgc cacattttgc gcggttatcg tctgtgcaat
    17581 ctttgtggcg agtttttctt tctggctggt gcagaacacc cttgccgaaa accagttcaa
    17641 ccaacgccgc accatcgaaa ccactttgat gggcagcatc atttccgcat tccgggcacg
    17701 cggggacgcg ggtgcgcgcg aaatcctgac ggaatggaaa gacagccccg tctcatcggg
    17761 cgtgtacgtt atacagggcg acgagaaaaa agatatcctg aaccggtata tcgacagcta
    17821 taccatcgaa cgcgcccggc ttttcgccgc cggacacccg cattccaacc tcgtccatat
    17881 cgaatacgac cgcttcggcg aagaatacct gttcttcacc aaagactggg acaaactcca
    17941 agcccgccgc ctgcccagcc ccctgttgat ccccggcctg ccgctcgccc cgatttggca
    18001 cgaactcatc atattgtcct tcatcatcat cgtcggactg ctgatggcat atatcctcgc
    18061 cggcaacatt gccaaaccca tcagaatctt aggcaacggc atggacaggg tggcaaacgg
    18121 agaacttgaa acccgtatct cccaacaggt cgacgaccgc gacgacgaat tgtcccatct
    18181 tgccatccaa ttcgacaaaa tggtggaaaa actcgaaaaa ctcgttgcca aagaacgcca
    18241 cctgctccac cacgtttccc acgaaatgcg ctccccgctt gcccgtatgc aggcaattgt
    18301 cggactgatt caggcgcagc cccaaaaaca ggagcaatat ctcaaacggc tggaaggcga
    18361 actgacccgc atggatacgc tggccgggga actgttaacc ctgtcccgtc tcgaaacttc
    18421 caatatggct ttggaaaaag aaagcctgaa actcctgccc ttcctgggca acctggtaga
    18481 agacaatcaa agcattgccc agaaaaacgg acaaacggtt accctgtctg ccgacggaaa
    18541 aatccccgaa aacacaacca tccttgccaa cgaaagctac ctgtaccgcg ccttcgacaa
    18601 cgtcatccgc aacgccgtca actacagtcc cgaaggcagc accatcctga tcaacatcgg
    18661 acaagaccac aaacactgga taatcgacgt taccgacaac ggccccggcg tggacgaaat
    18721 gcagctcccg cacatcttca ccgctttcta ccgtgcagac tccagtgcga acaaacccgg
    18781 aacaggactg gggcttgcat tgacccaaca tattattgaa cagcactgcg gaaaaatcat
    18841 cgccgaaaac atcaaaccga acggtctgcg gatgcgcttc atcctgccca agaaaaaaac
    18901 cggttccaaa acagaaaaaa gtgcgaactg accataatgc cgtctgaacc ggcttcagac
    18961 ggcattgcac aaacagttac cccaaaacaa acacacagcg ggtgcatctc caacaatcac
    19021 gcacataccg tccgcaaagg aatatatcat gtcggcacaa accgatccgg gctacttttt
    19081 catgcccaac cacattattc tgataggcgc gagcgaacgt ccgtacagcc tgggcgaacg
    19141 cgtactcagc aacctgctga gtacgccctt tcaaggaaaa atcacccccg taaacccgcg
    19201 ccaccacacc atagccgggc ttcccgccta caccagcctc aacaaaatcc ccggcaatgc
    19261 agacctgatt attgccgtta ccctacccga cagttacgac accctcttca aaacctgccg
    19321 caaaaagcag ctccgacaca tcatcctcat acaggactgg gacaacctgt ctaccgcaga
    19381 actgcacacc gccgaaactg ccatccgcaa acaccacggc aacggactca acatcaccgc
    19441 ctgcaccacc gcaggcatcc aactgccctc actcggactc aacatcagta cccaagacgg
    19501 atacgccgca ggccataccg ccatactgac cggcaatgcc gccgtcagcc gccaaatcga
    19561 caacatcctg aacaaactcc gtcaaggcac atcccgccac atcagcctgc atcccggcat
    19621 cagcctcatc acatccgccg attggctcaa ccgcttcgga cacagcctgc acaccaaaac
    19681 cgccgtccta caccacaacc ctgaagagga tcagcgcaaa ctgttcagcg caatccgcca
    19741 atttacccgc catacgccgc tgattctcca catcacctgc cgcacgacag aaaccgaccg
    19801 tgccgtactg cactgcctcg cccgacactg caacttcctc atcagtttca acgccgacga
    19861 cctcgaagcc gcactgcgcg cccaactgtc cgaccttcca cccctgtccc gactcgacat
    19921 cctgtccgac acgcctgccg aatggctgaa cgcgcacgcg ccaaaaaccc tcgccctcca
    19981 ctttcccaac cttccccacc acatccgcaa cggacacctg accggcacac ccacaccctc
    20041 aatctgccac gacatcgcct cacgtcagct tgcccgcccc gacacccaag ccgtcctgac
    20101 catcctcagc ccctccggac acgaggatta caaaaaaaca gcacgcgccc ttatccgcct
    20161 gtccgaacag accgccaaac ccctgctcgt cagcagcccc ttttcagacg gcataacaca
    20221 tttcgacacc cccactcagg caatccgcac cctttcctac cgcaacaccg ccgccgccct
    20281 gaaacaggca cagctcgaca ttgcaccgcc gcagccatgc cgtctgaaaa caccgcaacc
    20341 ccaaaacatc aaaaaagccc tcgaagccgc cgaccccgcc ctaatcgccg aagccctgca
    20401 cctccccccc taccggcaca ccacccataa cgccgtccaa ttccaattcg gcagccaccc
    20461 cctctatggc gacatcctga ccgcacgctg caacggacaa accactgccg tactcccgcc
    20521 gtttaccacg ctcgacagcc gccaccttgc ccgctttgcc gaactcgacg gcacacaaac
    20581 cctcgaccag ttcctgcaca cactgaccat cattcccgaa taccgccaac acattctcgg
    20641 catcaccctc aacctaaacg gcggacaata cagcagcgat ttcatcttaa agacacccga
    20701 aacacacgac acgcccaaac gcaagaacac aggcaaagcc gcccaaaccc tcgaacatgc
    20761 cgccgcaaaa atgcagagtg ccgccgcata cctgaaacac aaaaacccga cagccgccga
    20821 atttctccgc cacacaagcg aagccgccgc agaactgctc ggcagcaaaa ccgaaaccgg
    20881 agcagccgta cccaacgtac ttgcccccta tcccgcagca caccccaaaa cactgtccct
    20941 aaaaaacaac acgaccgtta ccattacccc ccttttgccc gaagacgcag aagccaaaca
    21001 gcagttcgtc cgcagcctcg gtcccgaagc acggtacaca cgcttcatga cccacaccaa
    21061 cgaactgccc tcagccacgt tggcacgcct gtgcaacccc gattaccact gtgaagccgc
    21121 atggacggca aaggatgccg acagcaacat cgtcgccgtc gtccgccaca gccgcctgaa
    21181 tcgcaacgaa tgcgaattcg gcatcacact ggcggaacat atgcgcggca gcggactggc
    21241 acagaaaatg atggaactca tcatccaaac cgccgcacag caaggctacc ggattatgag
    21301 tgccgacatt ctcaaaacca atacccccat gatcaaactt gctgaaaaat caggatttac
    21361 cctcaaggaa tcggacaccg aaaaaaacct gtaccgcgca tatctgaacc ttgcggcaga
    21421 caaaacaaca gaaaaaacaa ataaaaactt gcgcaccgac cacaaaataa cctaaaatcg
    21481 gcagtttcca tatatccgat tttctcaaaa ggactcaaaa tggtagttat ccgtttggca
    21541 cgcggcggct cgaaacaccg ccccttctac aacgtcatcg ttactgactc acgcagccgc
    21601 cgcgacggcc gcttcatcga acgcgtaggc ttctacaacc ccgtagccaa cgaaaaacaa
    21661 gagcgcgtcc gcctcaatgc agaccgcctg aaccactgga ttgcacaagg cgcgcaagtc
    21721 agcgactccg ttgcaaaact gattaaagaa caaaaagccg cctaatccgc gtttgccgcc
    21781 atgacagaca ctcaaaaccg ggtagccatg ggctacatca aaggcgtatt cggcataaaa
    21841 ggctggttga aaattgccgc caacaccgaa tattccgaca gccttttgga ctaccccgag
    21901 tggcatttgg tcaaggacgg caaaaccatc agcgttaccc ttgaagccgg aaaagtcgtc
    21961 aacagcgaac tccaagtcaa attcgaaggc ataaacgacc gcgacttggc attctcattg
    22021 cgcggttaca ccatcgaaat accccgtgaa gcattcgccc cgacagaaga agacgaatac
    22081 tactggacag acttggtcgg catgaccgtc gtcaacaaag accataccgt tttaggcaag
    22141 gtaagcaacc tgatggaaac cggcgcaaac gacgtattga tgattgacgg agaacacggg
    22201 cagattctga ttccgttcgt ttcccaatat atcgaaaccg tcgataccgg cagcaagacc
    22261 attactgccg actggggttt ggactactga tgcttatcca ggcagttacc attttccccg
    22321 aaatgttcga cagcattacc cgctacggcg taacgggacg cgcgaacaga cagggaatct
    22381 ggcagtttga agcagtcaat ccccgaaagt ttgccgacaa cagattgggc tacatcgacg
    22441 accgcccgtt cggcggcggc ccgggaatga ttatgatggc tccgccgctt catgcggcaa
    22501 tagaacacgc caaaacacaa tcctcccaag ctgcaaaagt catctacctc agcccccaag
    22561 ggaaaccgct gacacaccaa aaagcggtag aactggcaga acttccgcat ctgattctgc
    22621 tgtgcggacg gtatgagggc atagacgaaa ggcttctgca aagcagcgtc gatgaagaaa
    22681 tcagcatcgg agacttcgtt gtttccggcg gagagcttcc cgccatgatg ctgatggatg
    22741 cggtattgag gctcgtaccc ggcgtattgg gcgatatgca gtctgccgaa caggattcgt
    22801 tctcaagcgg cattttggac tgcccccact acaccaaacc cttagaattt caaggtatgg
    22861 ctgttccgga agtattgcgt tccggcaatc atggcttgat agcggaatgg cggttggaac
    22921 aatcgctgcg ccgcaccttg gagcgcagac ccgatctttt ggaaaagcgc gttttaatcc
    22981 caaaggaatc ccgcctctta gaaaccatcc ggcaagagca acgggaaatc caatcataat
    23041 ttaggaaaaa aacaatgaac ctgattcaac agctcgagca agaagaaatt gcccgcctga
    23101 acaaagaaat ccccgaattc gcaccgggcg acaccgtagt cgtatccgta cgcgtcgtgg
    23161 aaggtacccg cagccgtctg caagcctacg aaggcgttgt cattgcacgc cgcaaccgtg
    23221 gtctgaacag caacttcatc gtccgtaaaa tttccagcgg cgaaggtgtg gaacgtactt
    23281 tccaactgta ctcccctacc gttgagaaaa tcgaagtcaa acgccgtggc gatgtccgcc
    23341 gtgccaaact ttactatctg cgcggtctga ccggcaaagc tgcacgcatt aaagaaaaat
    23401 tgcctgcacg caaaggttaa taagcgattt cccccaatgc cgtctgaacc ttcagacggc
    23461 atttcttatt gctgcgatgc cggcaagtca acgatatgcc tgcccctcat ccaccttgat
    23521 taacaatcac tcccgcctat tcaagcataa tttacagacc aaacgttata cagtatcata
    23581 tcagccatat aacgataaac gctttaccgc cttccctttc aaaaccgcaa atcatcatga
    23641 caccttccct tttactatca ggattgactt tccgcctcat ccttgccctg attgccgtat
    23701 cccttttatg gggcgtttac ttttgggcag tatccgcatg agcatcattg tcgaaaacct
    23761 gaccgtcagc taccgccgcc gacctgccgt gcaccatgtg gacattactt ttgaagaaca
    23821 tagtatgtgg gcggttttcg gtcccaacgg cgcagggaaa tccacctttc tcaaatcctt
    23881 aatgggattg cagcctatcg atacgggcag catccggctg gacggattga cccgtcagaa
    23941 cattgcctac cttccccagc agtccgatat cgaccgctcc cagcctatga ccgttttcga
    24001 cttggcggca atggggctat ggtatgaaat cggctttttc aaagggataa ataccgctca
    24061 aaaacaacgc gttcacgaag ccttggagcg cgtcggaatg caacggtttg ccgaccgtca
    24121 gattgcctat ctctcaaacg gacaatttca gcgtgtcctt tttgcccgaa tgctggttca
    24181 aaatgccaaa ttcctgctgc tcgacgaacc cttcaatgcc gttgatgcac ggacaaccta
    24241 cgagcttctc gacgtattgc agaaatgcca ttgcggcgga cacgccatca tcgccgtact
    24301 gcacgattac gaacaagtcc gtgcctactt tcccaatacc ctgctgctcg cccgcgaaaa
    24361 aattgcggca ggcgcaaccg agaccattct gacagaaccc tacctcgccc aagccaacgc
    24421 caaaatgcag caacaggaaa gccccgagtg gtgcgcctca taaatgccgt ctgaaaccat
    24481 gtccccgtac caaacactat gaacccctac gacctgctta tcacaccctt tgcagaattc
    24541 gactttatgc gctacgccct cgcatccgtc ttctgcctgt ccctcagtgc cgcacccgtc
    24601 ggcgtattcc tcgtcatgcg ccgtatgagc ctgataggcg acgcattgag ccacgccgtc
    24661 ctgcccggtg ccgccgtcgg ctacatgttt gccggcttaa gcctgcccgc catgggtttg
    24721 ggcggcgtag ccgcaggtat gctgatggca ctgcttgccg gactcgtcag ccgcttcacc
    24781 accctgaaag aagatgccaa ctttgccgcc ttttatctca gcagcctcgc catcggtgta
    24841 gtcctcgtca gcaaaaacgg cagcagcgtc gatttgctcc acctcctttt cggctccgta
    24901 cttgccgtcg atattcctgc cctgcaactc atcgccgccg tatccaccct cacactgctt
    24961 acccttgccg tcatctaccg cccgctcgta ctcgaaagca tcgaccccct gttcctcaaa
    25021 tccgtcggcg gcaaaggcgg gctttggcac gtcctctttc tcgtcctggt cgtcatgaac
    25081 ctcgtatccg gctttcaagc cctcggcaca ctcatgtccg tcggactcat gatgctgcca
    25141 gccattaccg cccgcctatg ggcgaagcac atgggcgcac tcatcctcct atccgttctg
    25201 acagccctgc tgtgcggctt gagcggactg cttatttcct accacatcga aattccctcc
    25261 ggtcccgcca tcatcctctg ttgcagcgtc ctttatctct tttccgtcat actcggcaaa
    25321 gaaggcggca ttctgaccaa atggctcaaa aaccaccgcc accacaccac ctgaccccgc
    25381 aagccgcttg aacaaacagg cggcttgttt taccataaca gatgttacgt tatatcacag
    25441 gagaacagtt atgaaacacc tcaaactcac ccttattgcc gcattgctaa ccaccgccgc
    25501 aactgccgcc cccctgcccg tcgtaaccag cttcagcatt ttaggcgacg ttgccaagca
    25561 aatcggcgga gagcgcgtat ccatacaaag tttggtcgga gccaaccaag atactcatgc
    25621 ctatcacatg accagtggcg acattaaaaa aatccgcagt gcaaaactcg tcctgctcaa
    25681 cggcttagga cttgaagccg ccgatgtaca acgtgccgtc aaacagagca aagtatccta
    25741 tgccgaagcg accaaaggca tccaacccct caaagccgaa gaagaaggcg gacaccatca
    25801 cgaccacgat catgaccacg accacgaagg acaccaccac aaccacggcg aattcgaccc
    25861 gcacgtctgg aacgaccccg tcctcatgtc cacctatgcc caaaacgtcg ccgaagccct
    25921 gataaaggcc gaccccgaag gcaaagttta ttatcaacaa cgcttgggca actaccaaat
    25981 gcagcttaaa aaactgcaca gcgacgcaca agccgcattt aacgccgtcc ctgccgccaa
    26041 acgcaaagtc ctgaccgggc acgatgcctt ttcctatatg ggcaaacgtt accatatcga
    26101 attcatcgcc ccgcaaggcg tgagcagcga agccgagcct tcagccaaac aagtcgccgc
    26161 catcatccga caaatcaaac gcgaaggcat caaagccgtc tttaccgaaa acatcaaaga
    26221 cacccgcatg gttgaccgca tcgccaaaga aaccggtgtc aacgtcagcg gcaaactgta
    26281 ttccgacgca ctcggcaacg cgcccgccga cacctacatc ggaatgtacc gccacaacat
    26341 caaagccttg accaacgcga tgaagcaata aaaccgtttg attggtttga atttgaaaag
    26401 gcaaaaggcc gtctgaaaat tatagtggat taacaaaaat cagg
//
LOCUS       DAWHRA010000011        25458 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWHRA010000011 DAWHRA010000000
VERSION     DAWHRA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.011"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(105..1889)
                     /gene="lpdA"
                     /locus_tag="WIB30_000344"
     CDS             complement(105..1889)
                     /gene="lpdA"
                     /locus_tag="WIB30_000344"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ3796479.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMINTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRVPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1907..2344)
                     /locus_tag="WIB30_000345"
     CDS             complement(1907..2344)
                     /locus_tag="WIB30_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796480.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2438..4045)
                     /gene="aceF"
                     /locus_tag="WIB30_000346"
     CDS             complement(2438..4045)
                     /gene="aceF"
                     /locus_tag="WIB30_000346"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ3796481.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPAA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIMGDDIKAFVKSVMQ
                     GGAAKPAAASASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4195..6858)
                     /gene="aceE"
                     /locus_tag="WIB30_000347"
     CDS             complement(4195..6858)
                     /gene="aceE"
                     /locus_tag="WIB30_000347"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ3796482.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDTQLKSSGDREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVVT
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7552..9264
                     /locus_tag="WIB30_000348"
     CDS             7552..9264
                     /locus_tag="WIB30_000348"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ3796483.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERTAAQAELTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKVADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            9327..9983
                     /locus_tag="WIB30_000349"
     CDS             9327..9983
                     /locus_tag="WIB30_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ3796484.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9993..10448)
                     /gene="ruvX"
                     /locus_tag="WIB30_000350"
     CDS             complement(9993..10448)
                     /gene="ruvX"
                     /locus_tag="WIB30_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ3796485.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10441..10989)
                     /locus_tag="WIB30_000351"
     CDS             complement(10441..10989)
                     /locus_tag="WIB30_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ3796486.1"
                     /translation="MNLSNHFLIAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10991..11551)
                     /locus_tag="WIB30_000352"
     CDS             complement(10991..11551)
                     /locus_tag="WIB30_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ3796487.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENLDKR"
     gene            11879..13684
                     /locus_tag="WIB30_000353"
     CDS             11879..13684
                     /locus_tag="WIB30_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ3796488.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13804..15288
                     /locus_tag="WIB30_000354"
     CDS             13804..15288
                     /locus_tag="WIB30_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ3796489.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLNGAVGVSAAFLPPDALVVSTKGREG
                     TEGFALKARPEDYIFKAGTDPLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            15545..17644
                     /gene="uvrB"
                     /locus_tag="WIB30_000355"
     CDS             15545..17644
                     /gene="uvrB"
                     /locus_tag="WIB30_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ3796490.1"
                     /translation="MPSERRFRPIPTARFVRKRITGKIMEVIQYPNSPFKLHQPFPPA
                     GDQPTAIAGLLEGLSDGLAYQTLLGVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQ
                     LYAEMREFFPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNL
                     MTRNDVIIVATVSAIYGIGDPTEYQQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDF
                     KRGSFRVRGDVIDVYPAESSENALRISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPS
                     SHYVTPRDTVLRACESIKEELRERIEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCK
                     GIENYSRHFSGKKEGEPPPTLMDYLPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLV
                     DYGFRLPSARDNRPLKFHEFEKVMPQTIFVSATPAKYEEEHAGQVVEQVVRPTGLVDP
                     QIIIRPVATQVDDLMSEINDRIEKGERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSD
                     IDTVERVEIIRDLRLGLFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQ
                     TIGRAARNVNGVAILYADKITDSMKAAIDETERRREKQIKFNEEQGIVPQQIKKQVKD
                     IIDGVYHEEDGSKGRLKGKNKVKVGEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAV
                     LRDRIRDIKENLLFGAE"
     gene            17757..18119
                     /locus_tag="WIB30_000356"
     CDS             17757..18119
                     /locus_tag="WIB30_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796491.1"
                     /translation="MSQLDFNLELFIKNSNFDDYKIFDIVKEAIETQDEIGSILRTHL
                     FFERILEAWIYAKCNKEDFFNDTQIGFRTKLKIARNLGLFSEVYDIGERLNKIRNTIA
                     HHVLREVNQENLTLYMKV"
     gene            complement(18747..19463)
                     /gene="trmB"
                     /locus_tag="WIB30_000357"
     CDS             complement(18747..19463)
                     /gene="trmB"
                     /locus_tag="WIB30_000357"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ3796492.1"
                     /translation="MTDTAENQTQNNWQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(19585..20988)
                     /locus_tag="WIB30_000358"
     CDS             complement(19585..20988)
                     /locus_tag="WIB30_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ3796493.1"
                     /translation="MNNPDLPYRQALECLSQKQYNFTEVRRLLTEAFSAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALEWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKAAAQGLPEAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVEPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            21051..21152
                     /locus_tag="WIB30_000359"
                     /pseudo
     CDS             21051..21152
                     /locus_tag="WIB30_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(21211..23097)
                     /gene="uvrC"
                     /locus_tag="WIB30_000360"
     CDS             complement(21211..23097)
                     /gene="uvrC"
                     /locus_tag="WIB30_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ3796494.1"
                     /translation="MPSETVFQVIDMNKETRFPEHFDIPLFLKNLPNLPGVYRFFNES
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVREAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVRLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(23161..25323)
                     /locus_tag="WIB30_000361"
     CDS             complement(23161..25323)
                     /locus_tag="WIB30_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ3796495.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKAGKAEF
                     AELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
BASE COUNT         6023 a         6296 c         6931 g         6208 t
ORIGIN      
        1 gtcggagaag atattttaat ccgctgtaaa atagaaaatg ccgtctgaac atttcagacg
       61 gcattttctc aaataaacct tatcggctgt ttattcagtc ggatttattt tttcttttgc
      121 ggaggcaggt cggtacaagt acccaatgcc acttccgccg ccataccgat ggattcgcca
      181 agggttgggt gcgggtggat ggttttaccg atatcggctg catcacagcc catttcgatg
      241 gccaagcaga cttcgccgat catgtcgcca ccgttcggac cgacaatgcc gccgccgatg
      301 atgcggccgg tttcggcatc gaaaatcagc ttggtaaagc cgttgtcgca accgttggca
      361 atcgcacgac cggaagccgc ccacgggaag ttggctttgg tgattttgcg gccggaggct
      421 ttggcggaca actcggtttc gcccacccac gccacttcgg gggaagtgta ggcaacgccc
      481 ggaatcacgc gcgcgtcgaa gtaggctttg tggccggcgc agttttcggc ggcaacgtgg
      541 ccttcgtgaa cggctttgtg cgccaacatc ggctgaccga cgatgtcgcc gatggcgtag
      601 atgtgcggca cattggtacg catttgtttg tccacttcga tgaagccgcg atcggtaacg
      661 gcaacgcctg ctttttctgc gctgatgagt ttgccgttag gcacacgacc ggcggcaacc
      721 agtacggcat cgtaacgttg tggctcttta ggcgcatttg caccttcaaa ggtaacgtaa
      781 acaccatctt ctttaggctc gactgcaacg gttttggtgt tgatcatgat gttatcaaaa
      841 cgatattcgt tttgtttttg ccatacttta accaagtcgc ggtctgcgcc ttgcatcagg
      901 ccgtccatca tttcaaccac atccaaacgc gaacccagcg tgctgtaaac cgtacccatc
      961 tcgaggccga taatgccgcc gccgataatc agcagtttgc ccggtacttc tttcagagcc
     1021 aatgcgccgc tggaatcgat gatgcgcgga tcttcaggaa tgaaaggcag tttggttacg
     1081 cggctgcctg ctgcaatgat acagtttttg aaggcaacga tttttttctc gccggtaggg
     1141 gctgcctgtt cgtacgcgtc gccggcagtc agcgacactt ccaagtggtg cggatctaag
     1201 aattgcccgt cgccttggat aacgtccact ttacggcttt tcgccatacc tgccaaaccg
     1261 cccgtgaggc gggaaaccac gccgtctttg taggcgcgaa gcatatcgat gtcgagttcc
     1321 ggctcggggt atttgatacc gttggcagcc aagtggcgca cttcgtcgat aacggcggca
     1381 ttgtgcaaca aggctttgga agggatacag ccgacgttca ggcaaacgcc gcccaaagtt
     1441 ttgtaacgtt cgacgatggc gactttcaag ccttcatcgg cagcggcaaa tgcagcggag
     1501 taaccgccgg gaccgccacc caatacgacc acgtcgtact cggcatcggc agaaccgccg
     1561 aattgcgcgg cttgcggagc aggagcggca gctttagggg cttcttgcgc cggggcggca
     1621 gccgcttcgg ctttaggggc ggcagccgca ccttcagctt cgacgacgac aatcaaaccg
     1681 ccttcggaga ttttgtcgcc gactttgact ttgacttctt tgaccacgcc cgcgacttcg
     1741 gcaggcacgt ccatcgtcgc tttgtcggtt tccaaggtaa tcagggtatc gtccacagca
     1801 atggtgtcgc ctacatttac ttcaaccgca ataatatcta cattttcgtg tccgccaatg
     1861 tcgggcactt tcaattcaac taaagccatt atattgacct ttcaacttag ttaatttcta
     1921 tcaactcttt agcaataatc acttcttcag gaatacagcc acaatcttca gctctgattg
     1981 ttacttcctt ctcatttggg ttttctggat gttcaacctc atattcaaaa atagaatatt
     2041 gaccaaacaa atccctattt aaaacatata tatagccatt ttcgatgccg gaacttgttg
     2101 caaatttctt ggcaatttcc ttgtctgttg tcgtagatat ataacatccg tcatatagac
     2161 ctgtttcaat ttgatgggcg taaactgcat tcttcacact tggaccatgt gtagctttac
     2221 catcatattt aaacttacca tcataacgaa ttgcaacttc agctttatta cctttaggtt
     2281 ttaactgtcc attattttgc tcatcttgtt ggcaactaat gcctctatat aagaaatttc
     2341 ccataagtta ttaatttaaa ggttgaattt aacaccatta ctcatcaatc tcaagcaatc
     2401 caaacattat cagactgctt gagagggatg ttttatttta taaggtaatg cggcggaagt
     2461 ctttcaacag cttcgccaag aatacggtga agcgcatacc ggccgcaccg tcgatgacac
     2521 ggtggtcgaa ggacaggctc aacgggcaca tcaggcgcgg ggcaaactct ttgccgttcc
     2581 aaacaggttt gatttgggat ttgcacacgc ccaagatggc gacttcggga gcgttcacaa
     2641 ttggcgtgaa gcctgtgccg ccgatgccgc ctaagctgga aatggtaaag cacgcgcctt
     2701 gcatttcttg cggtttgagc ttgccttcac gggctttttt ggacaattcg gtcaattctt
     2761 ggctgatttg tttcaagcct ttttgatcca cgtctttgat gacgggaaca accaagccgt
     2821 tcggcgtatc ggctgcgaaa ccgatgttga agtagttttt cagcaccagg ttgtcgccgt
     2881 ccagtgaggc gttgaattcg gggaatgctt tcaacgcgga aacagaggct ttgatgatga
     2941 acgccaacgg ggacagtttc acgccttcgc gttcccattc tttgttcagc tgtttgcgga
     3001 attcttccag ctcggtcatg tccgcttctt cgtgtacggt aacgtgggga atcacaaccc
     3061 agttgcggga caggttttga ccggaaattt tcttaatgcg ggacaattct ttaacttcga
     3121 cattgccgaa tttggagaag tccactttag gccacggcag taagtccaga ccgccgccca
     3181 aagatgcgct ggctgcggca ggttttgccg cgccgccctg catcacggat ttcacaaagg
     3241 ctttgatgtc gtcgcccatg atacggcctt tcaagccggt gcctttgact tggcccaaat
     3301 ccacgcccaa ttcgcgcgcc agtttgcgtg cggaaggacc ggcgtgtgct ttggcgaaag
     3361 cggcctcgtc gattttggca gcggcaggtg cagaaggtgc aggcgcggcg gcgggtgcgg
     3421 cggcagcagt cggaggcgga gcggcagccg gtgcggcagc ttgagcggct tgagcagggg
     3481 ctgctgcggc agagccgacg gtttctactt cgataatggc agagccttcg gatactttgt
     3541 cgccgacttt taagaatacg gctttaacga caccggcagc ggtacaaggt acgtccattg
     3601 tcgctttatc ggtttccaaa gtaatcagcg tgtcgtcttc ggcaacggtg tcgcccactt
     3661 tgatttcaac ggcgattaca tccacatcgg tatggccgcc gatatcggga acggctactt
     3721 gaacggttgc accgcctgcg gcagcgggtg cggcagcagg tgcaggttgt gcttcggcag
     3781 cagccggggc ggcttcggcg gcggcggcac cggtttcaac ggtcagaatt acgccgcctt
     3841 cggagatttt gtcgccgact ttgactttca cttctttcac gacaccggcc gcatcggcag
     3901 gcacatccat cgtggctttg tcggtttcca gtgtaatcag ggtgtcgtca acggcgatgg
     3961 tgtcgcccgc tttaacttct acggcgatga tgtcgacgtt ttcgtgaccg ccgatatcgg
     4021 ggactttgat ttctacgata ctcatttgag ttcctttaat cttgttcaat tggatgccgt
     4081 ctgaaagctg cttcaggcgg catcggtacg gtttgtctgc tcaaaccatt cgggcattcg
     4141 gttttcagac ggcctgatgt cggaatgggg caaacagacc gtctgaaaca tcaatcagcg
     4201 tttccagcta ggagctgaat cggctttgat gccgtatttc tcaatggctt gttgaacggt
     4261 ttctttgctg actttgcctt gttccgccaa tgcgctcaat gctgccacgg caacgttgta
     4321 gcgatccact tcaaagaagc ggcgcaggtt ggcgcggctg tcggagcggc cgaagccgtc
     4381 agtacccaag acgtggtagt cgttcgggat gtaggcgcgg atacggtcag catagctgcg
     4441 gatatagtcg gtagcggcaa taaccggacc gtcatgacct tgcaatttgg aagtaacgaa
     4501 aggtactttt tcggcttcca gcggatgcag gcggttgaag cgttctacct cgacagcgtc
     4561 gcggtgcagc aggttgaagg atgggcaaga ccaaatgtct gcttccacgc cgaagtcggc
     4621 tttcagcagc tcggcaccgg taaccacttc ttgcaaaatc gtaccggaac ccatcagttg
     4681 gactttctta tcgcctttgc cgccggcttt cagcaggtac atacctttca agatgtcttg
     4741 ttccgcacct tcgggcatat ccggatgggt gtagttctcg ttcatcaggg tgatgtagta
     4801 gaacacgtct tcattattgg catacatacg gcgcagaccg tcttgtacga tgacggcgac
     4861 ttcgtattgg aaagtcgggt cataagatac gcagttcgga atcaggtcgg cctggatgtg
     4921 gctgtggccg tcttcgtgtt gcagaccttc gccgttcagc gtcgtacggc cggcagtacc
     4981 acccaacagg aagccgcgtg cgtgcatatc acctgccgcc caagccaagt cgccgatacg
     5041 ttggaaaccg aacatagagt agtagatgta gaaaggaatc atcgcaaagt tgctgttggc
     5101 gtagctggtt gcagcagcga tccagtcagc cattgcgccc ggttcgttaa taccttcttg
     5161 caagatttga ccgtcaacgg attctttata gaacatcagt tggtctttgt cttgaggggt
     5221 atattgctga cctttcggat tccaaatacc gtattggcgg aacatgcctt ccataccgaa
     5281 agtacggctt tcgtcgggaa cgataggtac gacgcgtttg ccgatttttt tgtctttcaa
     5341 caaggttgac aaaatgcgga caaatgccat agtggttgag aattcgcggt cgccgctgga
     5401 tttcagttgg gtatcaaatg cggacagctc cggcacttcc aaaacttctt gggtaggttt
     5461 gcgttgcggc aggtagccgc ccaaagcatc gcggcgtgcg tgcaggtatt tgtattcttc
     5521 cgtatcgggg gcaaaagtca ggtaaggcag atcgccgctt tcgatttgtt cgtcggtaac
     5581 cggaatgtca aagcggtcgc ggaattgttt cagggacgct ttgtccattt ttttggcttg
     5641 gtgggcaacg ttctgacctt cgccggatgc gcccataccg taacctttaa tggttttcgc
     5701 caagatgacg gtaggtttgc cgtcggcatg gttcgctgcg cggtcgtagg cgttgtacac
     5761 tttttgcggg tcgtggccgc cgcggttcaa tgcccagagt tgctcatcgg tcatatcggc
     5821 aaccaatgct ttcagttcgg gcgtattgaa gaagtgttcg cgcacatacg cgccgtcttt
     5881 ggatttgtaa gtttggtagt cgccgtccaa acattcttcc atacgttggc gcaggatacc
     5941 gtctttgtct ttcgccaaga ggcggtccca gcggcggccc caaatgactt tgacgacatt
     6001 ccagccggcg ccggcaaagt tgccttccaa ttcttggatg attttgccgt tgccgcgtac
     6061 cggaccgtcc aagcgttgca gattgcagtt gatgacgaaa atcaggttgt ccaagccttc
     6121 gcgtgcagcc agtgcgattg caccttgaga ttcgggttcg tccatttcgc cgtcgccgca
     6181 gaaacaccat actttacggc ctttggtttt tgccaaacca cgcgattcca agtatttcag
     6241 gaaacgcgcc tgataaatcg ccatgatggg ccccaagccc atggatacgg tcgggaactg
     6301 ccaaaagtcg ggcaagaggt gggggtgcgg ataggaaggc agaccgtgtc cgtccacttc
     6361 ttggcggaag ttgttcagct ggtcttcggt cagacggccc tcgacgaatg cgcgtgcata
     6421 gatgcccgga gcgacgtgac cttggaagaa gaccaaatcg ccttcttcgc cttcgccttt
     6481 ggctttccaa aagtggttga aaccgacttc gtacatggtg gcggcagatt ggaaagatgc
     6541 gatgtgccca cccagttcca aatctttctt gccggcgcgc aatacgatgg cggcggcgtt
     6601 ccagcgcacg aatgcgcgga tgcggtgttc gatgttttgg tcgcccggaa tgcctttttc
     6661 gttttcaacc gaaacggtat tcaaatacgg ggtggtcgtg ccgtgtggca tacgtacgcc
     6721 cttgtcgcgg cagtatttga ccaggttttc caagaggtat tgtgcgcgtt cgccgccttc
     6781 atattcgagg acggagctta acgcgtccag ccactcttgg gtttcgatag ggtcaacatc
     6841 gtgtaattgg gtggacatag tatctatcct ttatgttgag tgtttaatcg gcagggaggg
     6901 tgtagccaac ccctttattt tcgtttgtga aaaataataa gcaaaaacga aaatttctaa
     6961 atcaaaccga aaaaaataat ttaagtttaa tgttttcaaa aaattataaa agttttggaa
     7021 acaagggaaa acttgaaaaa aatgttcgat ctgctccaca cgaaatccta tcagcaatcg
     7081 ggtctgctgg caaataacca tgcctgtaaa agcaggtttt atactgaagt tggcgtttgg
     7141 taaggtcaag tctttttgat ttcgaaatat tctgtttgat tttggcgata ttttttcgga
     7201 attgtggtat atcgaatcat tatgtctctg tcggcaattt tcctattgta gaaaatctat
     7261 acacaagctt tgtggataac cgtgttgata agccacggct tgtgttaaaa aagtcttacg
     7321 caacaaatgt acgcttattt tgattaaaaa ataggcacat ttaaatctat aaaaatcatg
     7381 tgcttatgtt caatatgatg tttctggtca tgtttttgcc gtctgttatg ggatttgtcc
     7441 cgcgcttggg gcagggggta aattttggag gttggtgcaa aacctgcttt tttcgcgtta
     7501 caatctgcac ccgatttact tttgaatttg cctgtttgga aaaatacttt tatgaaagcc
     7561 agccaattct ttatctctac tttaaaagaa gcccctgccg aagccgcgct tgccagccac
     7621 aagctgatga ttcgcgccgg tctgattaaa gccaacgcgt ccggccttta tacttggatg
     7681 ccgatggggc tgcgcgtgtt acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgt
     7741 gcgggcagcg tggagttgct gatgccggtg gtgcagcctg ccgagctgtg gcaggaatcc
     7801 ggccgctggg agttttacgg taaagagttg ctgcgcctga aagaccgcca cgaccgcgat
     7861 ttctgcatgg gtccgacctg tgaagaagtc atcgccgaca tcgtgcgcaa agaaatcaac
     7921 agctacaaac agctgccgaa aaacttttac cacatccaaa ccaaattccg cgacgaagtg
     7981 cgcccgcgtt tcggcgtgat gcgcgcgcgc gagttcgtca tgaaagacgc ttattccttc
     8041 catgccgact acgcctcgct tcagacgacc tatgatgcca tgtatgacgc ttactgccgc
     8101 atcttcaccc gtctgggctt ggcattccgc cccgtcgccg cagacaccgg cagcatcggc
     8161 ggtacgggtt cgcacgagtt tcaagtgttg gcggaaagcg gcgaagatgt gattgcgtac
     8221 agcgacactt ccgattacgc cgccaatatc gagttggcac cgaccttgcc gcttaaaggc
     8281 gaacgcaccg ctgctcaggc cgagttgacc aaagtacata caccaaacgt caaaaccatt
     8341 gagtctttgg ttgaattcct gaatattccg gttgaacaaa ccctcaaatc catcgtggtt
     8401 gaaggcgaaa acgaaggcga aatcgtttta ttgctgttgc gcggcgacca tgagttcaac
     8461 gacatcaaag ccgaaaaact cgcaggtgtg aaatcgccat tgacgatggc aagccctgcc
     8521 gcgattgtcg aacagttcgg cgcaaacggc ggctcgctgg gtccggtcgg ctttgcaggt
     8581 aaagtttatg ccgatttcgc cactgaaaaa gtcgcggact gggtcatcgg tgcgaacgaa
     8641 gacggctacc actacaccgg cttcaacttc gggcgcgatg ccgccgagcc tgaattcgtc
     8701 gatttgcgta atgtggtcga aggcgacgaa agccccgacg gacaaggccg tctgaaactg
     8761 gcgcgcggca ttgaagtcgg acacgtcttc cagttgcgcg acaaatatac taaagccatg
     8821 aacgtcagct tcctcgacaa caacggcaaa tcgcaaatta tggaaatggg ctgctacggc
     8881 atcggcatca cccgcgtcgt tgccgccgcc atcgagcaga ataacgacga aaaaggcatc
     8941 atctggacca aagcaatggc gccgtttgaa gtcgttatcg tgccgatgaa ctacaaaaaa
     9001 tcagacaccg tacgcgaagc cgccgacaaa atctatgccg aattgctggc ggcaggcgcg
     9061 gatgtgctgc tggatgaccg cgacgaacgc gcaggcgtat tgctgaatga ctccgagctg
     9121 ttgggtattc cgcaccgcat cgtcatcggc gaccgcgctt tgaaagaagg caatgtcgaa
     9181 tacgccgaac gccgcaacaa cgaagcgcag gcagttgcaa tcggagaaat tgttgcgcgt
     9241 gtaacagctt cattaaatgg gtaagcttgg tcaaatgccg tctgaaaata atttcagacg
     9301 gcatttttat ttggggagaa agtgaagtgg cctcagtatt tttagaagga gaagcagtcg
     9361 aggtcggcaa tattttctgt atcggcagga actatgccgc acatattgaa gagttaaaaa
     9421 acgaaatacc ttccgaacct gtggtgttta tgaagccgtc aggcagcatt ttgaacagcg
     9481 gcggtacaat cttactgccc gagttcagta gggatgtgca gtttgagtgc gaactcgtgc
     9541 tgctggtcgg taaggattca gacggcacgg gcgagggtaa agatattttg ggatgtgtcg
     9601 caggatatgg tgtagggctg gaccttacgg cacgggatat ccaatgccgt ctgaaggaaa
     9661 aagggctgcc ctggctgaag gcaaaagggt tcaggcattc cgcgtgcgtg tcggattttg
     9721 cggcggcagg cagaatcggc aatcccgaga atgtgttgtt ttcattaaaa caaaacggcg
     9781 tattgaaaca gcgcggggat accggtttga tgatttaccc gattcgggaa attttacaca
     9841 aactggcggc agattacggt ttggggaaag gggatttggt gtttaccgga acgccctccg
     9901 gcgtgggcgc aatcggtgcg ggggacaatt tggcattgga gctggacggc ttggtgcgcg
     9961 cctctttcac ggtagggtgt taaccgcgcc gcttaacctt cacgtccgtt gaaacattcc
    10021 gccggaccgc cctcgaaaaa accgtgcaag atggcttgcg ccgccacttg gtcgagcacc
    10081 gatttgcgtt ttttgccgaa gacctgtgct tccgaaagca ggctttcggc atagacggac
    10141 gacagccgtt cgtcaaccca atagacgggg agattgaacc tgccgttcag cctgcgtccg
    10201 aacttgcgcg acaggtgcgt catttcatgt ttcgtgccgt cggtatgcac gggcagtccg
    10261 acgacaaaat aacgcggctg ccattcttga accagcttgg cgattgccgc gaacttttca
    10321 tcgttgctgc cgccggtaac ggtgctcaaa ggatgggata gccctaattc cgcgtctcct
    10381 tgtgccacgc cgatacgcgc ttcgccgaag tcgaatgcca gtgccgttcc ttttggaatt
    10441 ttatgcatgg ccggctcccg aaaacagggc gagcgggtcg atgccgagtt tggcgaatgc
    10501 ggcggcgtaa cggtgttcgt aggggatgtc gaacaggatg tgttcgtcgg cgggaacagt
    10561 cagccacgca ttgtcggcaa gttcgcgttc gagctgccct ttgctccagc ttgaatagcc
    10621 tatgctgatc aaggctttgt caaccgcacc ttcgcgtgaa atattttcaa tcacgtctcg
    10681 ggaagaagtt agcgcgatat tgtctgaaac gccgatactg ctttgccagt tgccgatcgg
    10741 ggtatgcacg acataaccgc gctcgacctg caccggaccg cccatcatca cgctgtcgtg
    10801 ctgcatccgc atggggatgt ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg
    10861 agagggtttg ttgatggcga tgccgagtgc gccgtcttca tcgtgtttgc agatatagac
    10921 gaccgattgt gaaaaaaacg cgtcttccat atcgggcatg gcgatgagaa agtggttgga
    10981 gaggttcata tcagcgtttg tcgagatttt ctatcgggtt ggaaatcatg caggcgccgg
    11041 caagcaggtt gccggaagta tcggcttgca ccccatcggt ttgcggtatg ccgccgccga
    11101 aacaggaaac cgcgcttaag gaatttttcg gcgagccttg gatgatttta tcgctgtaca
    11161 ccaaataggc gaaggttttg cgtttggggt cgtaataacg gacaatctgc cggctcttga
    11221 acgcgaagct cgcaccgtgt ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa
    11281 aagaaatcga agatgccgtc tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca
    11341 aattgaccat ttctttcaag ccgccttttt ttgcatacga aatataacag gcaacccctt
    11401 gaacgtcggg atcgtcgaat ccttccactt cgatacggtc gtttttgccc agcatgttga
    11461 aaacggtact tgcccgtccg attttatcgg tttcgccgct gccgcaggca gtcagcagga
    11521 cggcggcaga cagcagtagc agtctgttca tcaaaatttc cttatcggtt gaatccccgt
    11581 tttccgtgaa ggcggcaggg cttgctttgt ttggaggggc gcacgttccc aaaatcgggc
    11641 aacatacggt atgcggctta atgtgctacc ctgtgtgtcg gaacacaata tccgttgcgg
    11701 cattgaaaat atgacgcaca atataaggct gtttgagccg attgcaagta tccgcgcaaa
    11761 gaaggcgcaa gattgggcaa acggcatttt cagacggcat aactgacagt ataatccaaa
    11821 cgtccggctg ctttcgtgca gccttcagta ttatccaact ttttccgaag gtcgaaccat
    11881 gcgctacaaa ccccttctgc ttgccctgat gctcgttttt tccacgcccg ccgttgccgc
    11941 ccacgacgcg gcacacaacc gttccgccga agtgaaaaaa cagacgaaga acaaaaaaga
    12001 acagcccgaa gcggcggaag gcaaaaaaga aaaaggcaaa aatggcgcag tgaaagataa
    12061 aaaaacaggc ggcaaagagg cggcaaaaga gggcaaagag tccaaaaaaa ccgccaaaaa
    12121 ccgcaaagaa gcagagaagg aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg
    12181 ggataagaaa tcgaaggcgg aacacaaaaa ggcacatggc aagcccgtgt ccggatccaa
    12241 agaaaaaaac gcaaaaacac agcctgaaaa caaacaaggc aaaaaagagg caaaaggaca
    12301 gggcaatccg cgcaagggcg gcaaggcgga aaaagacact gtttctgcaa ataaaaaagt
    12361 ccgttccgac aagaacggca aagcagtgaa acaggacaaa aaatacaggg aagagaaaaa
    12421 tgccaaaacc gattccgacg aattgaaagc cgccgttgcc gctgccacca atgatgtcga
    12481 aaacaaaaaa gccctgctca aacaaagcga aggaatgctg cttcatgtca gcaattccct
    12541 caaacagctt caggaagagc gtatccgcca agaacgtatc cgccaagagc gtatccgtca
    12601 ggcgcgcggc aaccttgctt ccgtcaaccg caaacagcgc gaggcttggg acaagttcca
    12661 aaaactcaat accgagctga accgtttgaa aacggaagtc gccgctacga aagcgcagat
    12721 ttcccgtttc gtatcgggga actataaaaa cagccagccg aatgcggttg ccctgttcct
    12781 gaaaaacgcc gaaccgggtc agaaaaaccg ctttttgcgt tatacgcgtt atgtaaacgc
    12841 ctccaatcgg gaagttgtca aggatttgga aaaacagcag aaggctttgg cggtacaaga
    12901 gcagaaaatc aacaatgagc ttgcccgttt gaagaaaatt caggcaaacg tgcaatccct
    12961 gctgaaaaaa cagggtgtaa ccgatgcggc ggaacagacg gaaagccgca gacagaatgc
    13021 caaaatcgcc aaagatgccc gaaaactgct ggaacagaaa gggaacgagc agcagctgaa
    13081 caagctcttg agcaatttgg agaagaaaaa ggccgaacac cgcattcagg atgcggaagc
    13141 aaaaagaaaa ttggctgaag ccagactggc ggcagccgaa aaagccagaa aagaagcggc
    13201 gcagcagaag gctgaagcac gacgtgcgga aatgtccaac ctgaccgccg aagacaggaa
    13261 catccaagcg ccttcggtta tgggtatcgg cagtgccgac ggtttcagcc gcatgcaggg
    13321 acgtttgaaa aaaccggttg acggtgtgcc gaccggactt ttcgggcaga accggagcgg
    13381 cggcgatgtt tggaaaggcg tgttctattc cactgcaccg gcaacggttg aaagcattgc
    13441 gccgggaacg gtaagctatg cggacgagtt ggacggctac ggcaaagtgg tcgtggtcga
    13501 tcacggcgag aactacatca gcatctatgc cggtttgagc gaaatttccg tcggcaaggg
    13561 ttatatggtc gcggcaggaa gcaaaatcgg ctcgagcggg tcgctgccag acggggaaga
    13621 ggggctttac ctgcaaatac gttatcaagg tcaggtattg aacccttcga gctggatacg
    13681 ttgatcggat tgccgaaacg gtaggcttta tgcaaatcgt gccgatatgg tgcgctgttc
    13741 cgtgaagttt gccgtacctg tattttccgg tttttggaaa tcaagagaga aagttaaaga
    13801 agaatgtcga aacctgtttt taagaaaatc gcactttata ctttgggtgc aatcagcggc
    13861 gtggccgtca gtctggcggt gcagggtttt gccgccgaga aggacaggcg ggataacgaa
    13921 gtcctgccgg tgcaatccat ccgcacaatg gcggaggttt acggtcaaat caaggcaaac
    13981 tactatcagg acaaacccga tgccgatttg tttgaaggtg cgatgaaggg tatggtggcc
    14041 ggtttggatc cgcattccga atatatggat aaaaaaggtt atgccgagat aaaggagtcc
    14101 accagcggcg aatttggcgg cttggggatg gaaatcgggc aggaagacgg atttgtcaaa
    14161 gtggtttcgc cgattgagga cacgcctgcg gaacgggcgg gggtgaaaag cggcgatttc
    14221 attgtgaaaa tcgataatgt ttcgacgcgg gggatgacag tcagcgaagc ggtgaagaaa
    14281 atgcggggca agccgggtac gaagattact ttgacgctgt cgcgcaaaaa tgccgacaag
    14341 ccgatagtcg tcaacctgac ccgtgccatt attaaagtga aaagcgtccg ccatcacctg
    14401 atcgaacccg attacggcta tatccgcgtg tcgcagttcc aagagcggac ggtcgaaagc
    14461 gtcaataccg ccgcaaaaga gctggtaaag gaaaataaag gaaaaccgct caaggggctg
    14521 gtgttggatt tgcgcgacga ccccggcggg cttttgaacg gcgcggtcgg cgtgtcggcg
    14581 gcgtttttgc cgcctgatgc gctcgttgtc agtacgaaag ggcgggaagg tacggaaggc
    14641 tttgcgttaa aggcgcgtcc cgaagattat atttttaaag ccggcacgga tcctttggcg
    14701 ggtattcctg ccgagttgaa aaccataccg atgacggttc tgattaattc gggttcggct
    14761 tccgcgtcgg agattgtcgc aggtgcattg caggatcata aacgcgcggt tatcgtcggt
    14821 acgcagagct tcggcaaagg ttcggttcag actttgattc ctttgtccaa cggcagcgcg
    14881 gtcaagctga caacggcgct gtattatacg ccgaacgacc gttctattca ggcgcagggg
    14941 attgttcccg atgtcgaagt aaaagataag gaacgcattt ttgaaagccg cgaggcggat
    15001 ttggtcggac acatcggcaa tcctttgggc ggcgaggatg tgaacagtga aacccttgcc
    15061 gtgccgcttg aaaaagatgc ggataagccc gctgtaaaag aaaaaggtaa aaagaaaaag
    15121 gacgaggatt tgtcttcaag gcggattccc aaccctgcca aagacgacca gttgcggaaa
    15181 gctttggatt tagtcaagtc gcccgagcag tggcagaagt ctttggggct ggcggcgaaa
    15241 aagccggttt caaataaaga taagaaagat aaaaaagata agaagtaggt ttttacgatg
    15301 ccgtctgaaa ggatttcaga cggcatatta tgtttgaaag gacaggttat gagcgatttt
    15361 ttaccggaat ttgaaccgtg cggcatttat agtggattaa caaaaatcag gacaaggcga
    15421 cgaagccgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac tggtttttgt
    15481 taatccacta tacttgggta gcgattgggt ttcagacggc atacattcag gatttcaccg
    15541 ccttatgccg tctgaaagac gtttccgacc catccctact gcacgctttg tccgcaaacg
    15601 catcacaggc aagattatgg aagttatcca atacccaaat tcccccttca aactccacca
    15661 acccttcccg cccgcaggcg accagcccac cgccattgca ggcctgctcg aagggctttc
    15721 agacggcctt gcctatcaaa ccctgctcgg cgtaaccggt tcgggcaaaa cctacaccat
    15781 ggcgaacgtc atcgcccaaa gcggccgccc cgccatcatc atggcgcaca acaaaaccct
    15841 tgccgcccag ctttacgccg aaatgcgcga atttttcccc gaaaacgcag tggaatattt
    15901 cgtctcctac tacgactatt accagcccga agcctatgtg cccagccgcg atttgttcat
    15961 cgaaaaagac agcgcgatca acgaacacat cgagcagatg cgcctttccg ccaccaaaaa
    16021 cctaatgacg cgcaacgacg tgattatcgt cgccaccgtg tccgccattt acggtatcgg
    16081 cgacccgacc gagtatcaac aaatggtgtt gtccgtcaaa gaaggcgata ccatcgagca
    16141 gcgcgacatc atcgccacgc tcgtttccat gcaatacgaa cgcggcgatt tggacttcaa
    16201 acgcggcagc ttccgcgtgc gcggcgacgt gattgacgtg taccccgccg aaagctccga
    16261 aaacgcctta cgcatcagcc tgtttgatga cgaaatcgac cgcctcgata tgttcgaccc
    16321 gctttcaggc agccttatcc agcgcgtcgg ccgctacacc gtcttcccgt ccagccacta
    16381 cgttaccccg cgcgacaccg tcttgcgcgc ctgcgagtcc atcaaagaag aattacgcga
    16441 acgcatcgaa tttttcgccc gcgaacaacg ccccgtcgaa caacaacgca tcgaacagcg
    16501 cacccgtttc gacctcgaaa tgctctacga aatgggcttc tgcaaaggaa tcgaaaacta
    16561 ctcccgccac ttttccggca aaaaagaagg cgaaccgccg cctacgctga tggactacct
    16621 gcccgacaac gccatcatgt tcatcgacga aagccacgtt accgtaaccc aaatcggcgg
    16681 catgtacaaa ggcgacgcat cgcgcaagca aaacctcgtc gactacggct tccgcctgcc
    16741 ttcagcccgc gacaaccgcc cgctcaaatt ccacgaattt gaaaaagtca tgccgcaaac
    16801 catcttcgtt tccgctaccc ccgccaaata cgaagaagaa cacgccggac aagtggtcga
    16861 acaagtcgtc cgccccacag ggctggttga tccccaaatc atcatccgcc ccgttgccac
    16921 ccaagtcgac gacttaatga gcgaaatcaa cgaccgcatt gaaaaaggcg aacgcgtact
    16981 cgttactacc ctcaccaaac gcatggcgga gcaactcacc gactattaca gcgaactcgg
    17041 tatcaaagtg cgctacctgc acagcgacat cgacaccgtc gagcgcgttg aaatcattag
    17101 agatttacgg ctcggcctgt ttgacgtact cgtcggcatc aacctcttac gcgaaggcct
    17161 cgacatcccc gaagtctctc tcgtcgccat cctcgacgca gataaagaag gcttcctgcg
    17221 ctcccaccgc agcctgattc aaaccatagg ccgcgccgcg cgcaacgtga acggcgtcgc
    17281 catcctgtac gccgacaaaa ttaccgactc catgaaagcc gctatcgacg aaaccgaacg
    17341 ccgccgtgaa aaacagatta aattcaacga agaacagggc atcgtgccgc agcagattaa
    17401 aaaacaggtc aaagacatca tcgacggcgt gtaccacgaa gaagacggca gtaaaggccg
    17461 tctgaaaggt aaaaacaagg ttaaagtggg cgagattcac aacgaagaag atgcgattaa
    17521 agaaatcgcc aaactcgaaa aagccatgca gcaggcggct agggatttgc agtttgaaga
    17581 ggcggctgtg ttgcgggata ggattaggga tattaaagag aatttgttgt ttggggcgga
    17641 gtgagactta gactttgtcg tggatataaa aatagtagtt tattatttaa tttagatatt
    17701 agaataacta ttatattaac tagaatatag atttattttt gaaatgggca cttattatgt
    17761 ctcaattaga ttttaattta gaattattta ttaaaaattc aaattttgat gattataaga
    17821 tttttgatat cgtgaaagaa gctattgaaa cacaggatga aataggttca atcttacgaa
    17881 cacatttatt ttttgaaaga attttagagg catggatata tgcaaaatgt aataaagaag
    17941 attttttcaa tgatacacaa attggatttc gcacaaaact aaaaattgcg cgtaatcttg
    18001 gcttatttag cgaagtatat gatatagggg agagattaaa taaaattaga aatacaattg
    18061 ctcaccatgt actaagggaa gtaaatcaag aaaatttaac tctttacatg aaagtttaag
    18121 agattctttt aaaaataaaa tcatagtaaa ccaccaggaa tggaaaaatg gtgatatagg
    18181 tttaaaattt tataaagaaa atgaaaagga agaaatatct atatcatgga atgataaaag
    18241 cctaaagaag catatcatgc ttgctgtaat ctgcacttta actttgtcat atttagtcat
    18301 taaatcaaaa gatatatagc aacaagcgta ggtcggatac ttgtatccga caaaaccttc
    18361 aacattcccg tcgttgcaat ccattgcagc aatgcccaaa aggtcggatt cgagaatcca
    18421 atctacaaaa tcattacgag cataacacta tgaaatccct tcgtttttac cgcaatggcg
    18481 gcacttactt ttttacggtt gtaaccagca agccgtagcc tacatcacac caaccgcgtg
    18541 cgtagctatc acatactacc tgcgggcggc acaaacttta aaaccgatgt gcataaaacc
    18601 tataaggatg cgtgcggtac acacgcgttt tgccgttcaa actattgttt taaactttat
    18661 atcgaaacat cacttgctgc tttatcttga aaaaatgccg tctgtaagca aacatgtttc
    18721 agacggcatt ttcaaacagt ggtttgttat ccgatccgtt tgaataccaa gtcccaaacg
    18781 ccgtgtccga ggcgtttgcc gcgcgcttcg aatttggttt cggggcggta gtccggggtg
    18841 ggggcgtagt ctgccgccgt attttgcagg ctgtcgaagc tactgaggac ttcaagcatc
    18901 tgctgtgcat attcttccca gtctgtcgcc aggtggatat agccgccggt tttgagtttg
    18961 ggcagtagtt tggcgatgaa gggggcttgt atcagacggc gtttgtggtg gcgttttttg
    19021 tgccacgggt cggggaagaa tatgtggatg ccgtcgagcg agccgtcttg cagcatattt
    19081 tcgacaactt ctacggcatc gtgccgcatc acgcggatgt tttctaaatg gttttcgtct
    19141 atgagtttga gcaggttgcc tacgccggga ccgtgtacgt cgatggcgag gaagtcggtt
    19201 tcgggcaggc ggcgggcgat ttctgcggtt gccgtcccca taccgaagcc tatttcgagg
    19261 attttgggtc ggctgcttcc gaaacgggca tcaagatcgg ccggtgttgc ttggtagtcg
    19321 atgccgaagc tgtcccataa ggtatcgatg gcgcgttgct gcgcggcggt catatggctt
    19381 tggcggagga cgaagctgcg gatgctgcgg gggtgtccgg cttgccagtt gttttgtgtt
    19441 tggttctcgg cggtgtctgt cataatgctt tcgtacgctt gtgcaatcag gatgccgtct
    19501 gaatgtgaaa tgcctgttcg gacggcggga agggaaaggg gcgattatac ctgtttcgtt
    19561 tcggcagggt aggtgtaacg ctgttcagac ggcattttgc cattgttgca gcagttcttg
    19621 caggacgctt ttttgcccgt atccgttgtt tatggcttct tgcaggtagc ggcaggcttt
    19681 ttctttgtcc ggctcgacgc cgtgtccgct gtaatggatg caggcgaggt tgtagaacgc
    19741 catactgtct tttttcgcgg cggctttttc aaaccatttg cgtgcttcat tgtagtcggc
    19801 ggtcataccc tgtccgaagt aatacatgct gcccagattg gtttgggctt tgctgtgtcc
    19861 gagttctgcg gcttctgtgt agagtttgag cgcggctgcg tattcgggcg cgcattgtaa
    19921 tccgtaatgc agggcttggg ctttttgata aagccgttct gccctttgcc gcctgatggc
    19981 ggaatcttta atgccgccgt attggtcagg atggtttaac gcgctgtcgg atatgagttt
    20041 ctgataggcg gaaaggctgc cggcttcggc tgcctgccga taataatgcc gggctttttc
    20101 cgtatcgggg gctatgccca aaccgtagcg gcagatgtcg cccagtatcc gcaaaccttc
    20161 gggatggcgt tcggcggcgg ctgcttcggc atgatgcagg gcaagtttgt ggtttctttc
    20221 cagatgcttg ccttgcaggt agatgtcggc aagtgcggca tgggcatcgg ggtcattttc
    20281 ctgtgcggcc tgttgaaacc agtgaacggc ttcatcagga gccgtaggta ggatgagctc
    20341 ggcaagtggg gtgtaggcag gaatgtatcc ttgttgtgcg gcggcgcgca aatgattgac
    20401 ggcctgtgcc gtgtcgactt tcgtcccttg cccgaaacga tattgcagcc cgagctgcca
    20461 atgtgcctcc ggcaagcctt gtgccgccgc ttttcggtag agcaggtgcg ccgtttcaaa
    20521 atccggcggt gtgccgtacc ggcagtattg ggcaagcagg tattgcccgt gggggttgtt
    20581 gttttttgcg cttgtttttg cccattccaa ggcttcttca aaatgtcggg acgcgtacag
    20641 aaggtacatc aggcggactt gcgcttcggg cagtccttct tctgccaacg gtctgtaaag
    20701 cggtatcagg gtttccgggg gtgcgccttc caattcttgg atatatgcca gattgtaacg
    20761 cgcgtaggga tgtccctgtt cagcggcgat gcggagcatt tccatacctt gttcgcggtc
    20821 ttggtagggg ctgtccgcgt ccatcaggtg ttttgccaac tcgaatgcgg cggcgggatg
    20881 acctgccgag aacgcttctg tcagcagtcg gcggacttcg gtaaagttat attgtttttg
    20941 agacaggcat tctaaggcct gccgataggg taagtcggga ttgttcatgt cagatcgttt
    21001 tccgttttgt cgaatcatcg ttattcgtta tgaagttagg tttatagtgg attaacaaaa
    21061 atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggcaaggc gaggcaacgc
    21121 cgtactggtt taaatttaat ccactatata tcggtatacc gacctgctgt atcatatttt
    21181 ttacagacgg attttgtctt tgacagcatg ctaatgcaga tgattgtaaa tcgtttccgc
    21241 caatgccttg ctgatgcctt ccactttttc caagtcctcg cggctggcgg caatcacgcc
    21301 gcgcagaccg ccgaagcggg tgagcagggc ttggcggcgt ttgctgccta cgccggggat
    21361 gtcgcttaag gaggaggtaa cgcgggcttt gtcgcgtttt ttgcggtgac cggtaatggc
    21421 gaaacggtgc gattcatcgc gtacggtttg caatagatgc aaggccgggc tgttgggcgg
    21481 caggcggaag acttcgccgg taaaaggcag tatgagctcc tccataccgg ctttgcgctc
    21541 cgggcctttg gcaatgccga ccaaagggat gtgcagcccg agttcttccc ataccgatac
    21601 ggctacgccg atttgccctt tgccgccgtc aatcaacacg gcatccggcc atttgacggt
    21661 ctcgccgttg gcttcggcct cctgcatttt gccgtaacgg cgcgtcaaca cttcgcgcat
    21721 ggcggcgtag tcgtcgccgg gtttggcggt cgtgatgttg tagcggcggt attgcgaagg
    21781 ctggatgttt tgctcatcgt acacaacgca ggacgcaata gtggcttcgc cttgtgtgtg
    21841 gctgatgtcg aaacattcaa ggcggttgag gccgtctgaa tccatgccga ggatttttgc
    21901 cagttcatca atgcggtgtt gctggctgct ttgttgcagg cggcgttgtg caatcgccat
    21961 ttgcgcgttt tgttccgcca ttttcaaccg gactttgcgt tcgcctatgg tcttggtgac
    22021 aaattgcatc tgcttgccgt gttcgccttc caaagcctct tttagcgcat cgggaacggg
    22081 aaagttgctg atgatgatgt cgggtttgct tttgcccaga tagtgttggg cgacgaaggc
    22141 ttcggcgtaa tcttgtccgt ttggctcggg atcgtttttg gtgtcgggga aaaagctttt
    22201 gtcgccgacg tgccgtccgc cgcggatgct gacccagtgt acgcaaacca ggccgtctga
    22261 aaccgccagt gcaagcaaat cgatatcgtt gggattgttc ggatttttac tgtcgataaa
    22321 ctgattactt tgcatgatgc cgagcgcttg gatttgatcg cggtaacgtg cggcttcttc
    22381 gaattgtaga ttagcggcgg cggtttgcat tttgtgttgc agggtacgcg tcaattcgtc
    22441 agttttgcca ttaaggaaag tcgcggcttc acgcacgctg tcacgataat cttcttcact
    22501 gatgtggcct acacaaggcg cggtgcagcg tttgatttgg taaagcagac aaggacggtc
    22561 gcgatgctcg aatacactgt cttcgcaggt acgcagcata aagacttttt gcaacacttg
    22621 aatgctgtcg cgcacggcgt tgctgttggg atatgggccg aaatattgat taggcttttt
    22681 cagcgtgccg cggtaatacg ccatttgcgg atattgatgg ccgctgagca tcaaataagg
    22741 atagcttttg tcatcgcgga aaagaatatt gtatttgggc gacagggctt tgatgaagtt
    22801 gttttcgaga atcagggctt cggattcgga gcgggtgatg gtggtttcga tgtggtgaac
    22861 ctgtttcacc atcaatgcga tgcgcgggga atggtcgttt ttctggaaat agccggacac
    22921 gcgccgcttg aggttgacgg ctttgccgac gtataagacg ttgccgcttt cgttgaaaaa
    22981 acggtatacg cctggcaggt tgggcaggtt tttgaggaaa agtgggatgt cgaagtgttc
    23041 cggaaaacgg gtttctttat tcatatcaat tacttgaaaa acggtttcag acggcatatt
    23101 accatatcgc tggagacagc cttatccgtt cggacaaggc tgcagggcgg ttacatgctg
    23161 ctaatcgatt tttacccgtt tcaggcgcaa ggcattgctc aacacggaaa ccgagcttgc
    23221 cgccattgcc gcgccagcga tgacgggatt taaaaagcca agcgcggcga gaggaatgcc
    23281 caaaatattg tagaagaagg cgaaaaacag gttttgcttg atgtttttca aagtggcttg
    23341 cgacaccagc agagcatcgg cgagttggtt gaccgaatgc tgcatcagcg tggcggatgc
    23401 ggtatgttcg gcaacgtccg ctccgccttt catggcgaag ctgacgttag cggcggcaag
    23461 cgcgggcgcg tcgttgatgc cgtcgccgac catcgccacg gttttgccgg cggctttgag
    23521 tttttgcact tcggcagctt tatcgcgcgg actcatgttg ccgaaggcgt gtgcgatgcc
    23581 cagttgtttg gcgacgtatt cgaccgtgcc ttggttgtcg ccgctcataa tatagacatc
    23641 gatattgtgt tttttcagac ggcctatggc ttcggcggta tcggctttca acgcgtcggc
    23701 aagtgcgaat gcgccgatgg gtttgttatc gactgagacc gcaacaatgc ttgcaatatc
    23761 ccaaacgccg tctaaaaact tcggcaaggc cagttcggca aattcggctt tgcctgcttt
    23821 caccaaaccc acgccttcca cttcggcggt aatgcctgcg ccgacaacgg tttgtgcgtt
    23881 ttgtgcggcg ggaatgtcca aaccgcgcgc ttgggcggcg gagacgatgg cacgggcgag
    23941 cggatgggcg gcgttttgtt cgacggcggc ggcgatgcgg tacaaagcgt cttcgtcaaa
    24001 gccgctgtcg ggaacgcaat aaacggcggc aacctgcggg ctgccttcgg tcagcgtacc
    24061 ggttttgtcc aacacgacgg catcgacgtg ggcggcttcc tccattgctg ccgcgtcttt
    24121 aaaccaaata ccgtgtttaa ccgctttgcc cataccgacc ataatcgcgg caggggttgc
    24181 cagacccagc gcgcacgggc aggcaatcac caaaacggcg acggcgtgca tcagcgcaac
    24241 cgtccaatcg cccttaatca gccaagtaac aataaaagtc aacaacgcaa tgcccacgac
    24301 ggcaggcacg aataccgcag ccgctttatc ggctacgcgc gcaatcggtg ctttactgcc
    24361 ttgtgcttca gagagcgcgt tcatcatgtc gccgagctgg gtttggctgc cgagctgcgt
    24421 ggcgcggtac accacactgc cttcggtcat taacgcgccc gccaacactt tgccgcccgc
    24481 ctttttttct tcaggattgg attcgccggt aagatggctc tcgtccgccc aaccgctgcc
    24541 gctttcaatg atgccgtctg cggcaatgcg ttcgccgtgg ttggcgcgga taaggtcgcc
    24601 gatttgcact tggtcgatgg gaagctgttt ccattcgccg ttgcgttgca cgttgacttg
    24661 ggttggtgta agtttgagca gcaagcccaa gctgttgagg ctggattttt tggtacggtg
    24721 ttccaaaaat ttacccagtg acacaaaacc gatcaccatc acgcccactt caaaatacac
    24781 atgcgccata ccgtacgccg cgtgcgggct gaaaaacagc atatagacgg aatacaggta
    24841 aatcgagacc gtgccgatgg taaccagcac gtccatattc gccagtccgc ccttaatgct
    24901 cgcccacgcg cttttgtaaa acgggattgc cagccaaagc tgcaccacgc ttgccaatgc
    24961 gaactgccac aacggcggaa tcatccaatc gtgtctgccg atcatcatcc ccgccatgcc
    25021 gataaggaac gggacgttga tggtgaacag cagccacagc cgccagccga tatggtgttc
    25081 tgcttcgggt tgcggcaatg tatcttccgt tttttccttc gcgccgtaac cggttttctc
    25141 aatgattttg gcaatgtcgg ctactgaggt tttgctgtcg tcaaacacta cctgcgcctc
    25201 ttcgctggcg aagtttaccc ccgccgattc gacaaaatct tttttgttca acactttttc
    25261 aatgcgcgaa gcgcaggcct ggcaggtcat gccttcgatt tggaaacgga ttttttgttg
    25321 cataagcgtg attcctgctt tgatattcag acggcagtta aggctgcggg atgccgtctg
    25381 agcgccggta tggtcgaaat ttctcaaacg cgccgataat tcgagtagaa tccgaaaact
    25441 tgacacagca aaggaaaa
//
LOCUS       DAWHRA010000012        25352 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWHRA010000012 DAWHRA010000000
VERSION     DAWHRA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.012"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..920)
                     /locus_tag="WIB30_000362"
     CDS             complement(<1..920)
                     /locus_tag="WIB30_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ3796496.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANNTLLIMRPYQ
                     IAAAERILWKINSSALAKNWSKPESGGYVW"
     gene            complement(1350..1922)
                     /locus_tag="WIB30_000363"
     CDS             complement(1350..1922)
                     /locus_tag="WIB30_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ3796497.1"
                     /translation="MGEVFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENGRILDNS
                     LQHISKSAVKGGKLFPANSIIMATSATIGEHALITVPFLANQRFTSLSLKPEFADKLS
                     IYFLYYYCFNLSEWCKKNTTTSSFASVDMNGFKRFPIPIPPLPEQEKIVAILDKFDTL
                     THSISEGLPHEIALRRKQYEYYREQLLAFR"
     gene            complement(1963..2559)
                     /locus_tag="WIB30_000364"
     CDS             complement(1963..2559)
                     /locus_tag="WIB30_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ3796498.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGDS"
     gene            complement(2725..3735)
                     /locus_tag="WIB30_000365"
     CDS             complement(2725..3735)
                     /locus_tag="WIB30_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ3796499.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(3732..5282)
                     /locus_tag="WIB30_000366"
     CDS             complement(3732..5282)
                     /locus_tag="WIB30_000366"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ3796500.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSVSKLIARLAVHGQ
                     EKVNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYN
                     KFHIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKS
                     KADFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNL
                     FYGTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADV
                     PHIAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREID
                     EVIAEIEA"
     gene            complement(5355..6359)
                     /gene="rfaD"
                     /locus_tag="WIB30_000367"
     CDS             complement(5355..6359)
                     /gene="rfaD"
                     /locus_tag="WIB30_000367"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ3796501.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEEFIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(6400..7518)
                     /locus_tag="WIB30_000368"
     CDS             complement(6400..7518)
                     /locus_tag="WIB30_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ3796502.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFYKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDISDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KSYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(7520..8755)
                     /gene="dcm"
                     /locus_tag="WIB30_000369"
     CDS             complement(7520..8755)
                     /gene="dcm"
                     /locus_tag="WIB30_000369"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ3796503.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFSGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGAKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(8792..9763)
                     /gene="hldA"
                     /locus_tag="WIB30_000370"
     CDS             complement(8792..9763)
                     /gene="hldA"
                     /locus_tag="WIB30_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ3796504.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(9818..10558)
                     /gene="pyrF"
                     /locus_tag="WIB30_000371"
     CDS             complement(9818..10558)
                     /gene="pyrF"
                     /locus_tag="WIB30_000371"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ3796505.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(11084..11731)
                     /gene="adk"
                     /locus_tag="WIB30_000372"
     CDS             complement(11084..11731)
                     /gene="adk"
                     /locus_tag="WIB30_000372"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ3796506.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            11947..12786
                     /gene="htpX"
                     /locus_tag="WIB30_000373"
     CDS             11947..12786
                     /gene="htpX"
                     /locus_tag="WIB30_000373"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ3796507.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(13087..13647)
                     /locus_tag="WIB30_000374"
     CDS             complement(13087..13647)
                     /locus_tag="WIB30_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796508.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(13761..14153)
                     /locus_tag="WIB30_000375"
     CDS             complement(13761..14153)
                     /locus_tag="WIB30_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3796509.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(14361..14753)
                     /locus_tag="WIB30_000376"
     CDS             complement(14361..14753)
                     /locus_tag="WIB30_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3796510.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(14870..15280)
                     /locus_tag="WIB30_000377"
     CDS             complement(14870..15280)
                     /locus_tag="WIB30_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3796511.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(15294..15731)
                     /locus_tag="WIB30_000378"
     CDS             complement(15294..15731)
                     /locus_tag="WIB30_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3796512.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(16142..17440)
                     /locus_tag="WIB30_000379"
     CDS             complement(16142..17440)
                     /locus_tag="WIB30_000379"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ3796513.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGVGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(17544..18695)
                     /locus_tag="WIB30_000380"
     CDS             complement(17544..18695)
                     /locus_tag="WIB30_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ3796514.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVVDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            19030..20409
                     /gene="norM"
                     /locus_tag="WIB30_000381"
     CDS             19030..20409
                     /gene="norM"
                     /locus_tag="WIB30_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ3796515.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGTVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            20484..21524
                     /gene="murB"
                     /locus_tag="WIB30_000382"
     CDS             20484..21524
                     /gene="murB"
                     /locus_tag="WIB30_000382"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ3796516.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLCDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVELANADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVIGAEKAAALLEQHPDMPHYPQPDGSVKLAA
                     GWLIEQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            21536..22186
                     /locus_tag="WIB30_000383"
     CDS             21536..22186
                     /locus_tag="WIB30_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ3796517.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            22241..23038
                     /locus_tag="WIB30_000384"
     CDS             22241..23038
                     /locus_tag="WIB30_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ3796518.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAVKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHANKPVWFCLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDEIALPDPQTESARQILAAEQHLLDSGVPNI
                     DILRLGGLYCAERHPVGRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKN
                     IIEPRHPTRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            23089..23655
                     /locus_tag="WIB30_000385"
     CDS             23089..23655
                     /locus_tag="WIB30_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ3796519.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            23675..24565
                     /locus_tag="WIB30_000386"
     CDS             23675..24565
                     /locus_tag="WIB30_000386"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ3796520.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            24590..25348
                     /locus_tag="WIB30_000387"
     CDS             24590..25348
                     /locus_tag="WIB30_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ3796521.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
BASE COUNT         5928 a         6537 c         6214 g         6673 t
ORIGIN      
        1 tgccaaacat agccgccgct ttcgggctta ctccaattct ttgccagagc cgaactgttg
       61 attttccaca aaatgcgttc ggcggcggca atctgatacg gccgcataat cagcagcgta
      121 ttattcgcat caaacacgct gtaatgcagc aaaacgctca gcaatacgct tttctgcagg
      181 aacgtggcgg taaagtcttt caaatcctta atcggatgat tgtccgaccg cgcccaattc
      241 atcgtgaaat cgaagctatt tttgtcgcgc ttggtggtgt tggcgaaata gcgcgtgtcc
      301 gtgccgttgg aaatcacgaa gatttgcagg aatttgaaca gcgaattttc gctgttgaag
      361 ctctctttgc tgtaacggtg cacctgattg aatgcctcgc gcaccgccac accgcgcttt
      421 ttcaattcaa tctgcaccag cggcaggccg tttaccaaca cggtaacgtc gtaacggttt
      481 gcatgcgtgc ccgtctgctc aaactggttg ataagctgca catggttgcg ggcaaggttt
      541 ttcttgtcca gcagataaat gtttttcaga cggccgtcat caaaagcgaa atcgtaaata
      601 tggtcgtcgt ggattttgcg ggttttatcg gtaatgtttt cagacggcct gtccagatat
      661 tccgtcaaaa accgcgccca ttcgccgtct gaaaacgcca catcgttcag ccgctgcaac
      721 tgcgcgcgca ggttttccag cagcctgctt tggctgttca aatccttgcg gtattcgtaa
      781 ccctgattct gcaaatcggc gattaactcc gcttccaacc ggttttccga ttggtagctg
      841 cccgactgtt cgattttttc atattggtcg agcacgatga aattcggcgt ttcggcgatg
      901 ggtttggttt cgaggttcat agttcgtcct ttgcagggtc gggttttaaa cagtttgatg
      961 ttcggattgg gctgcctgaa aatgtgcagg ctgctttggt cggcttcggc ggaatgcggg
     1021 gtggttgtgt ccgccgtagg gtgggcttca gcccaccgcc ccacctgccg ttgttttact
     1081 gttgttgcag cttgctgctg tttcggtggg ctgaagccca ccctactttg tctgcgactt
     1141 ctaccccacc gatgccgtct gaatcatccc aatcgggttt ttctctttgt tttgagcatc
     1201 tatcgccata ttttccggtt tccacacgct gcggcggaat gcggggcggt tgccgctcac
     1261 cgtagggtgg gcttcagccc accgccccgc ctgcccttgt tttactgttg ttgcagattg
     1321 ccgttttttt cggtgggctg aagcccaccc taccgaaagg caagcagctg ttcgcggtaa
     1381 tattcgtatt gtttgcggcg cagggcgatt tcgtgcggca ggccttcgct gatggagtgg
     1441 gtcagggtat cgaatttgtc gaggatggcg acgatttttt cttgttcggg gagtggaggg
     1501 atggggattg ggaagcgttt aaaaccattc atatccacag aagcaaaact cgatgttgtc
     1561 gtattctttt tgcaccattc actaagatta aaacaatagt aataaagaaa ataaatgctg
     1621 agtttatcgg caaattcagg ctttaaagat aaactggtaa atctttggtt ggctaagaat
     1681 ggaacggtta ttagtgcatg ctcacctatg gttgcagatg tagccatgat aatagaattg
     1741 gctggaaata gcttaccgcc ctttactgcg ctttttgata tatgctgtaa tgaattatct
     1801 aaaatacgac cattttccct aatatcttcc attctaaacc aagggattga gccattttcc
     1861 caatattctt tatttgattt ggaaggggta tagccattct tcaagtcaaa cacctccccc
     1921 aacgtcttcc aaaccacatc tttcagacgg cattgatagc catcagctat cccccccgat
     1981 ttgattgtca aaatctaaaa gaaagtcgcg gtaataccgg tattggcgtt tgcgcagggc
     2041 caattcagct tccagctcgg tgaatttgtc aagtattttt acaatttttt gttgggtttc
     2101 caggggtggg atagggatta ataatttttt caagttactg gcatttagag caggaatacc
     2161 tgtaaatgta cataaggcat gaatctcttg ttcaaattca agtaatatat gatagagaaa
     2221 acgaacatcc aattctgttc tattcttaat agtaactgca taatttaagt tgccacgaaa
     2281 atatcttcct tcttgccacg ttattgagcc gactcccgct cctcttgttg tgatgccgat
     2341 agggtcattt tcagtgttcc attcatcaat atagccaagc ggctctttgc cactgttaat
     2401 aactggataa ctaccaatat tatttgagat tttctgctta cttacagctt gtcctgtttt
     2461 tatgatagct atcccatttt ctccacccaa cggcttccac tccaccggtg ccgtctgaat
     2521 catctcaatc aattttttcg ctttgctttg catatccatg gttgttttcc gtttctttcc
     2581 cgctgtggcg ggatgtgggg cggttgtggg gtgcttcggc tcactctgtc cgtttgccgt
     2641 tatttctgcg ggctgaagtc cgtcctgctt tcaggctgcc tgcaatggtg tgcaggctgc
     2701 tttttcaggt agcctgaatg gtagttattt tgtgcggtct tttttctgtt tgatctgttg
     2761 cacggctgtt ttttccaacg ctttaacgct ttccagatag gcttgctcca ccggcgacag
     2821 gatgcgcgct tcataggcgc ggtattcgcg ttcggctttc tgctctgcct gtttgcggct
     2881 gatgctgcct gcaccctgta atgtgccttg tccgtacagc ccggaaaatt tgtccaattc
     2941 ggctatccag tcgcgcatat acatggggct ttgctcctgc gctttgattt ccgctaggtc
     3001 gaagaaggcg gaaaccagac ggttcaggcg gaacagttcg tcttcggtca gatagttttt
     3061 ggcgattttg gcttcattca gcgtggggat tgcgccttga aaggtggtca gccccataaa
     3121 gtctttgctg ctgtcggcac ggctgtatat cagctcagct gcggtttgcc ggctggcggc
     3181 atagtgcagt ttgttttgaa cggcggcaaa aaaggtttgg ctttcgctgc ttttggggtt
     3241 gtagtcttgg ctggtggcat ataaatcaag cacttgccgg tatagggctt tttcgctgct
     3301 gcggatgtcg cgaatgcggt tgagcagttc tttccaatag tcgccgccgc ctgtgccttt
     3361 caggcgttcg tcgtctatgg caaagccttt ggtcagatat tcgtccagcc gttcggttgc
     3421 ccattggcgg aattggatgc cccgcgcgga acggacgcgg tagccgacgg caataatcat
     3481 gggcagggaa taatgggcga ttttgcggtt taccttgcgc ccgttttcat tttgaactgt
     3541 caactggaag ttgacagttg ccttctcttc caattcttgc tctgcaagaa tggttttaat
     3601 gtgtttgctg atattttgtt tggtggtttg gtacagttct gccatgtccg cctgcgtcag
     3661 ccaaagctgt ccgccaaatt cctgcaaggc aaattgggca gtgccgtcgt tggcggtgta
     3721 taggatgatg ctcatgcttc aatctctgca atcacttcgt caatttcacg ccgcagccgt
     3781 tcgattttgg caacggtttc gctgatttcg gcgttaagct gtttgatgtc gataatttcg
     3841 cgggtgtctt cgggttcaac atagctgctg acggcgaggt tgtagccgtt gtctttgacg
     3901 gtttgctggg cggcgttttg ggcgatatgc ggcacatcgg ctttatcggc gaagagtttg
     3961 acgatttcgg caatgtgttc ttcggttaag acgttgttgt tggtttcttt tttaaagaag
     4021 ccgcctgcgt cgatgaattg gatgtcggta ttgtctttgt gtttggacaa aaccagtata
     4081 ttgacggcga tgccggtgcc gtaaaagaga ttgggcgcaa gggcgatgac ggtttccacg
     4141 tagttgccct ccaccagata ttggcggatt ttctgctctg cgccgccgcg atagaaaatg
     4201 ccggggaatg agacgatggc ggcgcggcct ctgccggaaa ggtagttcag tgcgtgcaga
     4261 atgaaggcaa aatcggcttt ggatttcggg gcgagtacgc ctgcaggggc aaagcggtcg
     4321 tcgttgatta aggtggggtc gccgctgcct atccagttga tggaataggg cggattggaa
     4381 acgacggcat caaagggttt gctgtctttg agtttgggat tggtcagtgt gtcgcccaat
     4441 tcgatgtgga atttgttgta attgacgttg tgcagaaaca tattcatgcg ggcgaggttg
     4501 taggtggtgt ggttgatttc ctgcccgaag aagccttctt cgatgatgtg ctcgtcaaac
     4561 tgttttttcg cctgcaagag caggctgccc gagccgcaag ctgggtcgta gattttgttt
     4621 actttctcct gcccgtgcac cgccagccgc gcaatcagct tggatacgga tacgctttgc
     4681 ggggtgaaaa attcgccgcc ggatttgcct gcgttggcag cgtagttgga aatcaggtat
     4741 tcgtaggcat cgccgaaaag gtcgatgtgg tggtcttcaa aactgccgaa atcgagttcc
     4801 gccacgcctt ttaggacggc ggcaaggcgt ttgttcttgt cggcaacggt gctgccgagc
     4861 cggctgctgg tggtgtcgaa gtcgtcaaac aggcctttaa tgtcttgttc ggacggatag
     4921 ccggaggcgg agctttcaat cgcggtaaaa atttctttca gcttggtgtt gagctcttcg
     4981 ttttgatggg cttcggcggc aatattgcaa aaaagctggc cggggtagat gaaatagcct
     5041 ttgactttga cggcatcgtc tttgatttcg ggcgtgatga tgctgtccgg catagcggcg
     5101 taatcaatac tgctgtcgcc tgcctgcata tagtcggtaa agttttcgct gataaagcgg
     5161 tagaaaagtg tgccgagaac gtattgttta aagtcccagc cgtccaccgc gccgcgtact
     5221 tcgtcggcaa ttttccaaat ttggcggtgc agttgggcgc gttgttgcat ttcggtcatc
     5281 atcgaaatcc atataaaagt taaacaaatc gaaatcgcct gatattttca gacgattttt
     5341 tacgggcatt caaattaagc caaattttcc agcatccatt tgacgtagcg gtcgacgcct
     5401 gatttgacat cgaaaaattc ttccttatat ccggcttcgc gcaatttggt gatgtcggct
     5461 tgggtgaagc tttggtattt gcctttgagc gcgtcgggaa atgggatgta gcggataaat
     5521 tcttcttcta ccaactcttt taaactcatt tcaggtttgc cttcggcggc gcggcaggcg
     5581 ttgacggtgg cagcggcgag ttcgttaaac tgttggctgc gacctgttcc taggttgtag
     5641 atgccggaaa gttcgggatg gtcgaagaag tagaggttga ctttggcaac gtcttcgacg
     5701 ctgacaaagt cgcgggtttg ttcgccgttg ccgtagccgt cgttgctgcc gaacaggttg
     5761 acgtaaccgt gttcgcggta ttggtggaaa tggtggaagg cgacggatgc catacggcct
     5821 ttgtgttgtt cgtgttgtcc gtacacgttg aagtagcgga agccgacgac ttgggcggtc
     5881 aagccttctt tcatacggcg acgcaatact tggtcaaaca ggaatttgga gtagccgtac
     5941 acgttgagcg gtttttcgag ttcgcgctct tcgcggaaga tttcgccttt gccgtaaacc
     6001 gccgcactgg aggcataaag gaaggggatg cgttcgtcct gacaccagtc cagcaaatcc
     6061 agcgtgtact ggtagttgtt gtccatcata tacaaaccgt cgtggttcat cgtatcggaa
     6121 cacgcgcctt gatggaaaac ggcttcgatg ttttgataag gtaaaatgtg ttccctcact
     6181 tggcggatga attcatgttt atcgagataa tgggcgattt cgcactcggc aaggtttttg
     6241 aatttttcgc ctttgctcaa attatcgacg gcaacaatgt cagtaatacc gcgttgatta
     6301 agtgctttga cgatgttgct gccgataaag ccggccgcgc ctgttacgat gatggtcata
     6361 ttcggtttcc tttgttttga gtaaaattaa taaacttagt tacaagaaat catcgatatt
     6421 gtcctgaaac acaaatttgc gatgatattc caaatatttt tctcttcctt cgggatatat
     6481 tctcatctta ttttttgcat ttggattgat attaagtttt tcccatgtgt aacggcttag
     6541 tcgagtaccg caaatcaaac gaccatcatc caagaaagta atataacctt gatcgaacaa
     6601 ccaatcataa gtcggcgtta aagctaaccc atttaaataa tctaatgcct ctttctcatt
     6661 tttctcagta atacaaacca tataagattt aatatgacta gcccttaaca aaatctcatc
     6721 tgtaattaat gtaaatggac attgcggcat atgatttata acatccttgc gatatttctg
     6781 ctgccctttt ctacttttct gtttaatgat ttcttgctct acgaattgat taaattctga
     6841 agccggtatt tctaattttt ctcttgataa gagttgcggg tgcacgatag agcgaaattg
     6901 ataatccaaa aatattcgga aataaaataa tggttctgaa tcttctatat ctttgacagg
     6961 caatagttta agaattgata aataactaat tttgggcaat acaatcttac gccaaattga
     7021 ccatatatca tcctccgaac ggatataacc cctacggtaa cctttagaat tttccaaatg
     7081 gtctgatata tcatggattg aaaagaaaat attttctgta ggcagattat taatacttaa
     7141 aacgtaatca tcataaaacc ttgaaatatc atgatgatat ttctgttctt gtttgtgata
     7201 ttcaactttt gcatcaacca aataacgctg taaatttttc ttataaaaaa aacaattttt
     7261 ctgaatgaaa ggatattcta ccctccttcc attttttgtc tcagtaatcc cccaattttc
     7321 atactcaaaa aatgtactta gttttcgacc actatcatca atataactac ctacatattt
     7381 gcgtgcttcc ccattaccac caaaaacaga taacttgtta ttcctgtgaa taaaactatc
     7441 ttccgcacgt aaattttgaa tactatcgac aataaaatac tctatgtttt caatagtaat
     7501 tctttgaaaa tccggcattt tactctccat aaagcgtaat atccatttgt tttaatatag
     7561 cgattaatac atcaacaaca atactgtttc ccgcttggcg atacatttga gtatcactaa
     7621 ctaaaatttt aaaatcatct ctaaaaccca ttagccgcaa acattcccta ggtgttaatt
     7681 tacgaatacg tccacgatta tgagtaacat aattatcaac cccggcacga tgcattttat
     7741 gcatagtttg caataatggg cgagctacag gcaaatcagt ttccgtactg gttttaaaat
     7801 tttttgttcc tcctgccaaa acataatttt tgattttttc agaaagataa tatttttcct
     7861 caacgttatt tacatcaaaa ataaagtcat caaattcaga ttcgcgggct gcctgaaaaa
     7921 taaaatcacc atgccaatta aattgttgat ttgctttttg gcataaggca atttctccat
     7981 taatctgtgt ataacgtttt tgtctatttt tagaactggt aacaaatttc gcaccctttt
     8041 cacgtaaaaa atatttgcta tcagtatagt cctccaaaaa atcttgcata gtatgttcta
     8101 attcaatctt ttcaggaaac tgaaaaccat ttataggagg ggtatgaaag ccaacaacaa
     8161 aaatacgctc acgatgttga ggaatcccat aatccttact attcattatt tggaaatata
     8221 agtcataacc aagtgaataa aaaacacttt ttacaacttt ccaagttttt ccattatcat
     8281 gattaagcaa gccctttaca ttttcataaa taaatatctt tggttggact tcatccacaa
     8341 cgcgagcaaa ttcatagaat aatgttcctc gtgtatcttc taatcctgca cgtttgccaa
     8401 ccatggaaaa tgcttggcaa ggactgcctc caactaaaat atcaacttga tttctaaact
     8461 ttctcgcatc aaattgagta atgtcgttat gccaaaaatc ttcatttaat ttatagtttc
     8521 caagataact ttttttaacg tatggatcaa tatctcctga aaaaacaatg gtatggttta
     8581 aatttaatcg gtggaatgcc tgttcaaccg caccaatccc gctgaatacg gttgctaatc
     8641 taatatgtga atcaggttta agaaaagttt tagatttcca accttgttga ctgggaaaga
     8701 gcaaagtttt ttgtaatcga gtatcgtgtg tctgtgccat tgtcgaaata gtcatactta
     8761 tatcgttctg tttatcttat caatatgaaa actacattgt tgattgccct gacaatgcct
     8821 tgatcaattc ggcaaacgag caaaccgccg taccgagttt cgccacgaca accccggccg
     8881 cagtattggc aaggtacatg gcttcgggca tggtgcagcc tgccgccaaa cccaagccca
     8941 ttccggcaat gacggtgtcg cccgcaccgg atacgtcgta aacttcttgg gcgcgggtgg
     9001 gctggtaaat cggttcgcct tcgctgaaca aggtcatgcc ttcttcgctt cgggtcagta
     9061 aaacggcggt caggtcgagg tggcggcgca ggttttgcgc tttttcggtc agctcgcttt
     9121 cgtttttcca actgccgacc acttctttca attcggcgcg gttaggcgta atcagagttg
     9181 caccgacata tttttcgtaa tcgtcgcctt tggggtcgat taagacggtt ttgccggcgt
     9241 gtttcgccca atcgatcata tcggagatat gcgacaggcc gcctttgccg tagtctgaaa
     9301 aaatgattgc gtcgtattcg ggcaagattt cgcggtattt ctgcttgatt tgttccaaca
     9361 cttcgcagtt gggatgttct tcaaaatcaa gacggataag ctgctggttg cgggcgacga
     9421 cgcgcagttt gacggtggtg gcgatttgtt tgtcgcgcat cagataggag gcgacgccgt
     9481 cctgcaccat cagcgcatcg agcgcgtcgg cggcttcgtc gttgccggtt acggacaaca
     9541 gccctgccct gccgcccaac gaagcgatgt tgcgcgcgac atttgccgct ccgcccgcgc
     9601 gttggtcgat tcgtccgatt ttcgccaccg gcacgggggc ttcgggcgaa atacgggaca
     9661 catcgccgaa ccaatagcgg tcgagcatca cgtcgccgac aaccaggact ttggcttgcg
     9721 cgaaacggga tttgagggtt tcttgttgga acttggcgga catattgcct ccgttgtatt
     9781 ttttcagacg gcctcagcct gtaaggccgt ctgaaaatca gtttgcttca aggtttgcca
     9841 cgcggttcac ttcgcgcaat acggctaccg gatcggcagc ttgggttaca ggacgaccca
     9901 ttaccaaata agtcgaaccg gcagccaagg cttcggccgg tgtcatgatg cggcgctggt
     9961 catcattatt gccggcaacg tccaagcgga tgccgggcgt gaccaagaca aaatcctgtc
    10021 ccaattcgcg gcgcagcggc gcggcttctt gggcggaaca gaccacgccg tccaagcccg
    10081 aactttgcgc cagttttgcc aagcggatga cttgttcttc aggggcggtg ttcaaaccga
    10141 tttccgccaa atcactttgt tccatgctgg tcaacacggt tacgccgatt aagagcggct
    10201 tcgtgccgta tccggcaacg gcttctgctg cggcttccat catacggcgg ccgcccgatg
    10261 cgtgcatatc gaccatccaa acgcccatat cggcagcgac tttgcaggct tgcgcgacgg
    10321 tgtggggaat atcgtggtat ttcaaatcga ggaaaagttt gaaaccttga ttgattaagc
    10381 tttctgccaa attgcgtccc gtcgcggtaa acagctcttt gccgattttg atttgacaca
    10441 atgtcgggtc aaggttgcgg acgaatccga gcgtgtcttt ttcgttggaa aaatcaaggg
    10501 cgacgataac gggggtgcgt tgttgcggag tttggaagtc ggagattaaa ggattcatgg
    10561 tgttttcgtt tcgggttttc agacgacctt tatcgtcttt ttcgcagtat agtggattaa
    10621 ctttaaaccg gtacggcgtt gtctcgcctt agttcaaaga gaacgcttct ctaaggtgct
    10681 gaagcacaaa gtgaatcggt tccgtactat ctgtactgcc tgcggcttcg tcgccttgtc
    10741 ctgatttttg ttaatccact ataacaacgt aaattcaaac cgtaggtcgg gcattgatgt
    10801 ccgacctacg cggactcaag cctccgacaa actgtttcgg ataaatttga accggtacat
    10861 cagccgtcat tcccgcgcag gcgggaatcc attgataaaa ctcagttcat cagatttaaa
    10921 cagcgattgc ccaaattcag aaatggattc ccgcctgcgc gggaatgacg ccaaatgatt
    10981 atttttcggt tgcaacacgc tgtttatcaa aaacaggtcg tctgaaaacc tgattttctg
    11041 ttttcagagt gcgaagcctg cccgtgggta cgaccttttc gatttatttg cccaatgcgc
    11101 ccaatacttc ggctttcacg gcttctactg cttgggtgcc gtcaactttg atgtatttag
    11161 gcgcgtgttc gccttccagt ttgctgtaaa aatcgaccaa aacttcggtt tgctcgtggt
    11221 aaacggcaag gcgttttttc acggtttctt ctttgtcgtc gtcgcgctga atcaaatctt
    11281 cgccggttac gtcgtctttg ccttcaactt tgggcgggtt gtaggtaacg tggtaagtac
    11341 ggccggaagc caaatgcacg cggcggccgc tcatgcggtc gacaatcacg ctgtcaggca
    11401 catcgatttc aacgactgca tccaaatcca cgcctgcttc aaccatcgct tcggcttgtg
    11461 ccaatgtgcg cgggaaaccg tcaaacaaga aaccgttttt gcagtcgtct tgcgcgatgc
    11521 gttctttgac catgccgata atgatgtcgt cgcgcaccaa gccgccttcg tcaatgattt
    11581 ttttcgcttc caaacccaag ggcgtgcctg ccttaatcgc ggcacggagc atgtcgccgg
    11641 tagagatttg cggaatgccg aacgctgcgg tgatgaattg cgcctgagtg cctttgcccg
    11701 cgcccggcgc gcctaaaagt aatgctttca tgaagtgatc ctttgaaatg tgtttattgg
    11761 aatcaaacag atggtttacg attctacacg gcttggcaca atagttcaaa aaccaactgc
    11821 acaaactgcc atttgcaagc aaatgttttg catacctttg ccgagtctga tattcacaag
    11881 ccggcaactt tttgggacaa tcccccaaaa tttcaaaaca tcacacccta ccaaaaagga
    11941 acatccgtga aacgaatttt tctgtttttg gctaccaata tcgctgtttt ggtcgtaatc
    12001 aacattgttt tggcggtttt gggcatcaac agccggggcg gcacgggcag cctgttggcg
    12061 tattccgccg tcgtcggctt cactggttcg attatttcgc tgctgatgtc caaatttatc
    12121 gccaaacaat cggtcggcgc ggaagttatc gacacgccgc gcaccgaaga agaagcctgg
    12181 cttttgaaca ctgtcgaagc ccaagcgcgg caatggaacc tgaaaacgcc cgaagtcgcc
    12241 atctaccact cccccgaacc caatgccttt gccacgggcg catcgagaaa cagctccctg
    12301 atcgccgtca gcaccggttt gctcgaccat atgacgcgtg acgaagtgga agccgtattg
    12361 gcgcacgaaa tggcacacgt cggcaacggc gatatggtta cgctgacgct gattcaaggc
    12421 gtggtcaata cctttgtcgt gttcctgtcg cgcattattg ccaacctgat tgcccgaaac
    12481 aacgacggca gccagtccca gggaacttat ttcctggtca gcatggtatt ccaaatcctg
    12541 ttcggcttcc ttgccagctt aattgtcatg tggttcagcc gacaacgcga ataccgcgcc
    12601 gatgcgggcg cggcaaaact ggtcggcgcg ccgaaaatga tttccgccct gcaaaggctc
    12661 aaaggcaacc cggtcgattt gcccgaagaa atgaacgcaa tgggcatcgc cggagatacg
    12721 cgcgactccc tgctcagcac ccacccttcg ctggacaacc gtatcgcccg cctcaaatcg
    12781 ctttaaaccg atttgaaacg gcaaaaaccg tacgcgcaag cagtacggtt ttttgtatat
    12841 gctgtctgaa aacatcaggt ttccataccg acaccaaccg tatccatttc cgcaccggat
    12901 acgccgcatt gatataccct gataaaccgt atccggacag aaaatatagt ggattaacaa
    12961 aaaccagtac ggcgttgcct cgccttagct caaagaaaac gattctctaa ggtgctgaag
    13021 caccaagtga atcggttccg tactatttat actgtctgcg gcttcgtcgc cttgtcctga
    13081 tttttgttaa tcccctatat cagataaaac agcggtaaaa ttgccgtctg aaaccaactc
    13141 tgcgccatac gggacacgct ctgcacgtct atttgcctcc gatatatcct ccctactgta
    13201 ggaacgtctg ccgatttgca cgaaatcggc acctctagca cccttcagga tggcggacgt
    13261 tatccgttct tgggattctg caagacggtc gacggcatta tacagacgct cggtctgata
    13321 cccttccgaa gcaacctgct tcaggtattc caaataaccg taacccaacg cccctccgac
    13381 aagcagggtc gtcaacgcta ccagcgttgc cgaacggatt ttagggggca tatcttgaag
    13441 catatctcta ccgaaccgaa ccaaagtctc tttaaagtcg gactctacac gcgtagaacc
    13501 ctcggaaatc ttgaagttca actcatactg cttccgtttg tccgctgtca accgcttgag
    13561 gttgtcatcc ccgtattgga cggttgacac aaccctgtac agactgtcct gtatttcgac
    13621 cagcgtctgt gcaaagttgg cggtcataac tggattccca acccttgccg ccgattctga
    13681 tactcaaaac ggtatccagg cgttccagca gggcttcggg agacagcagt gcgtattctt
    13741 caatattttc cataggttga ttatagcaga accttatacc tgttacagac aaggttgtcg
    13801 ggatttgaac gtccgtcgag cttggttcgt atagagccgt agggggtgtt tagggctttg
    13861 gcggcagctt gtacgctggg gaacgtgagt gtttcgcctg tggcggtgtc gaggatggag
    13921 acaggttttt ggttttgggg aggcgtattt tccttcattt taagtttccc gcactccgga
    13981 cacccatact ttgactttat agctgtgtcg taatacaaaa gttggacaac cccgtgcgta
    14041 ggacatctaa cactggaagg tttataaatg ccactgaact ccaacaattc taaatgtgga
    14101 aatttctctt gaaagcgtga tttggcggta tcgaaagtca ttcgcttacc cataatttgt
    14161 ctccataggt tatgtgcaaa tggattacac aaccaaaata tagaaatttt caaatttttt
    14221 gagacctacc tgaaatcctg cgcaagcaca atgtaaagaa aaatggtata cgtctttact
    14281 aaatctttac ataaattaac tctaaccggg atttgaacgt ccgtcgagct tggttcgtag
    14341 gatttagggt gttgtgaacc ttacctatcg tacccaagca ctttgtacct gtttgaaata
    14401 aggttgtcgg gcgacgttcg acccttgagc ctgtgattga tttgttggaa atgtacacct
    14461 aaagcagcac ctgcggcagt aacgcttggg aacgtgagtg tttcgttggt cgttgtgtcg
    14521 aggatgagga ggggcttccc tacatttgga ggtgtttggg tttttgacat cttagcacac
    14581 tcaggacacc cgtactttga ctttataagg gcattaaatg tcgaataacg aacgttcccg
    14641 tgcataggac aattaacggt tactgggtaa ttcacaccgt caaagccggt tagagagata
    14701 ttagggaata tatcagtaag ttcagaagct gcttggttga aagatttggc catttgagac
    14761 tccaaaattg attgtcaaat ggatttaata gcaaatttat aaaaatgtca aatttttcga
    14821 gaagcacctg atagtggcga ggggtataca ctgttaaaaa gtgtatacgt catttagtgc
    14881 agatgtgaac cttatacctc cctgcaagta aagtctctgt gtgaacccga ccttttatac
    14941 ggtcagtgat agctgctggg tttataccca atgaccttgc agcagatgct cttgagggga
    15001 atgtaagtgt ttcgccagtt acagtatcct ccaatactgt atttttagat aatttatcta
    15061 ttgtatcttc tggaattttg tgggaattga caccataaga tgcacatttt ggacacccac
    15121 ttttagagtc caacatactt ttgaatgtag agcaagtaac cctcccgtgt ataggacaga
    15181 cgattaccgt cgggtaacga acgccattga actccacaag attcaaatgt ggaaatttag
    15241 aggttagctt agaaactgct tgagtaaatg taagtgccat agtggttgtg aacttacttt
    15301 accaacctat aacgatacct aatcaatcgg tcaaacggag tgcgtccgct taaacgtaca
    15361 gctaaagtgc tgttggagca accgataaat ttactggcag ccagtgtact tgcaaactcg
    15421 tagatttccc ctgtctctgt gtcctccaac ctaatggcaa cggcgtttgg agagggcttt
    15481 tcgggatttc gagtacattc aggacatcct gttgaggatt tcatggcagc ttgtagattc
    15541 ccgtaaacaa ccgacccatg caaagggcat tggattgtta ccggttcgta gtttttagtg
    15601 aacttcaaaa ccgtcttatc aggaaattta ctggaaaacc ttaactgaac atcttgtagc
    15661 gagagtgatt ttggcattat agacctttct aaaatgaaag cattatacaa ccaaaatata
    15721 gaaattttca aattttctct gaacgcactg gtatccgatg gagctaaatg taaagaaaag
    15781 ggtatatgtc cttactaaat atttacacat tttaactgta tagtgtttga caaacaacag
    15841 gcataggcgc ataatgcacc cgtagttaga tagatagtaa ggagataacc aatgacttag
    15901 cacctgcccc cctctctctc gcgcaccccg cccctcgctt gcggctaatg ggctttgcgc
    15961 gacatataca ctttcatctc ctgcatggct taaccgccta tgcaatagta cacacatcaa
    16021 caaatcaggc tggcacgtct tggcattggc tgacgtacca ttgtagcccg aaatgttgat
    16081 accctactat ttatgaccaa cggcggatta gggcttcaga cggcaatttt actgcaaaac
    16141 cttatgcgaa cggatgatgc agcacaatcg tttcttctcg gtcggggccg gtggagacga
    16201 tggcgaccgg cgcgccgcag acttcttcaa tccgtttcaa atatgctttg gcgttttcgg
    16261 gcaatgcgcc gtagtccttc acgccgaaag tggattcgcg ccagccgggc atggtttcgt
    16321 aaatcggctt gcaggtttcc accgcatcgg aaccgcaagg caggatgtcg gttttgccgc
    16381 cgtcgggcaa ttcatagccg acgcagatat tgatggtttc aacgccgtcc attacatcga
    16441 gtttagtaat acacataccg gaaatgccgt tgatttggat ggagcgtttc agggcggcgg
    16501 catcaaacca gccgcagcgg cgtgcgcgtc cggttaccga accgaattcg tgtccgcgtt
    16561 ctgccaaacc tacgcctact tcgtcgaaca attcggtcgg gaacgggccc gaaccgacgc
    16621 gcgtggtata ggctttgacg atgcccaaaa cataatccag catttgagga cctacgcccg
    16681 cgcctgccga agctgcgccc gccagacagt tggacgaggt aacgaaggga taagtgccgt
    16741 agtcgatgtc caacaacgca ccttgcgcgc cttcaaacag cagtttttcg ccgtttttgt
    16801 ttttctcgtt caacacgcgg gacacgtcgg taatcatcgg cgcaatgcgc ggcgcgactt
    16861 tttcgataac cgccatcacg tcttccgctt taaccggctc ggcattgtgc agatgttgca
    16921 gttggacgtt gtaataagca aggacggcat ccagtttttc acgcagtttt tcaggatgca
    16981 gcaaatcggc ggcgcgaatg gcgcggcgtg ccactttgtc ttcgtaggca gggccgatgc
    17041 cgcggccggt cgtgccgatt ttgcctttgc cgcgcgatgc ttctcgggct tggtcgagcg
    17101 cgatgtgata aggcaggatc agcgggcagg tcggcgcgat tttcagacgg ccttcgacgt
    17161 ttttcacgcc tgccgcgttc aactcgtcga tttcgcccaa cagggcttcg ggggagacga
    17221 caacgcccga accgatgaag cagtccagac tttcatgcag gatgccgctc ggaatcaggc
    17281 gcaaaatggt ttttttgccg ccgacaacca aggtatgacc cgcattgtgg ccgccttgga
    17341 agcgcaccac gccgccggct tcttccgcca gccagtcaac gattttacct ttaccctcgt
    17401 cgccccactg tgcgccgatt actacaacat ttttagccat agccatataa cctatcgata
    17461 ttaaaaatta ttgtgggaat ccccaaaccg gtgcggcttt gcgccccgcc acgatttgcc
    17521 tttcatccgc catgaacggg tatttagcgt ttcacgacct gccaaacgcc gtccgtcttt
    17581 ttcagacggc ctgcaagctc ttccgaaaca ttgtgtccga taccgtagtc aaccacgaca
    17641 cactgccctt gttcacgcaa ggcttcgacc gcttcgcgcg ccgcttcggc atcttccgca
    17701 tcgaccaaca cggcgggctg ccgttcgatg gcgggcaaac gcccgataaa gctgcgcaag
    17761 tcgaaactga atcccgttgc cgggcgcgcc ctgccgaaat atccgcccaa tccgtcataa
    17821 cgcccgccgc gcgcgaccgc gtcgtggaaa tcggcggcat aggcggcata cagcaagccc
    17881 gtgtggtaat tgtcgacacg cagctcggac aagtcgatat ggatttcaca atcggggaat
    17941 gcgtcgcaca ccgcctgcaa ttcgcccaac gcgccgccga ccgccgacaa atccggcaac
    18001 cgtccgcgcg cgtcggacaa cacttcacgc ccgccgtaca ggcgcggcaa cagcgagaat
    18061 gcttttgccc acatgccgtc cagcttccaa gccttgacct gcgcttcgac cgccccggta
    18121 tctttatcct gcatcaaggc aagcagcgtt gcggactgcc ccgcatccaa atgtgccgca
    18181 tcggacaagg cgcgaaatat gccgatatgc cccagcgaaa gcagcacttt gcccatatcg
    18241 gcaattttca tgcttttcag cataaggtct atcagctcga tgtcgccacg gatgtcggca
    18301 aaaccgtaca tttccgcccc tgcctgcaag ggttcgcgca tattcagcag accgtcggac
    18361 tgcgcgtgca acaccggacc ggcataacac aaccgattaa tcccttggtt ggcggataaa
    18421 agatgggcat cgatacgcgc cacctgcggc gtgatgtcgg cgcgtatgcc caactgcctg
    18481 ccgctgagcc tgtccgttac caaaatggtt ttcagggaaa gccccgcatc gatatgcgtc
    18541 agcagggaat gtgcgtactc catcagcgga ggctgtacca gttcataacc gtgtacgcgg
    18601 aacagtgcca acaactgctc cctcgcgctt tcaagctgcc gcgcgttcgt gggcagtacg
    18661 tcggcgatat gttccggaag ctgccatgtc tgcatgagaa tctgccttct attttatcat
    18721 gtaaatacaa aaggctgccc gatccgcaat cagacggcat ctttgccgca acgcatcacg
    18781 ccgctgtcaa aacggcgatg cttccttccg attcagaatc cgaacagccc ctgtccgacg
    18841 gctcaatgcc gtcaagtcgt taaaaccaaa ctttaccata aaatacacac aatctgaata
    18901 tacttttata ccctgtatcc gccgtctgac ggcacggcat ttttttacta tataatgtgc
    18961 gccattattt ctcatcactt cctgctgccg ttttctgctt cagacggcat ttttgttgat
    19021 tgaacactta tgctgctcga cctcaaccgc ttttcctttt ccgtcttcct gaaagaagtc
    19081 cgcctgctga ccgctcttgc cctgcccatg ctgttggcgc aggtcgcgca ggtgggcatc
    19141 ggttttatcg atactgtgat ggcgggtggt gcaggcaagg aagacttggc ggcggtggct
    19201 ttgggcagca gcgcgtttgc cacggtttat attaccttta tgggcattat ggcggcgctg
    19261 aacccgatga ttgcccagct ttacggcgcg ggtaaaaccg acgaagtggg cgaaacgggg
    19321 cggcagggga tttggttcgg gctgtttttg ggcgtgttcg gcacggtctt gatgtgggcg
    19381 gcgattacgc cgttccgcaa ctggctgacc ttgagcgatt atgtggaagg cacaatggcg
    19441 cagtatatgc tgttcaccag cttggcgatg ccggcggcaa tggtacaccg cgcactgcac
    19501 gcctacgcct ccagcctgaa ccgcccgcgc ctgattatgt tggtcagctt tgcggcgttt
    19561 gtgttgaacg tgccgctgaa ctatattttc gtttacggca aattcggtat gcccgctttg
    19621 ggcggcgcag gctgcggcct ggcgacgatg gcggtgtttt ggttcagcgc gctggcattg
    19681 tggatttata tcgccaagga aaatttcttc cgcccattcg gactgacggc gaaattcggc
    19741 aaaccggatt gggcggtgtt caaacagatt tggaaaatcg gcgcacccat cgggctgtct
    19801 tattttttgg aagccagcgc gttttcgttt atcgtgtttt tgattgcgcc tttcggcgag
    19861 gattatgtgg cggcgcagca ggtcggcatc agtttgtcgg ggattctcta tatgattccg
    19921 caaagcgtcg gctcggcggg gacggtgcgc atcggctttt cgcttgggcg gcgcgaattt
    19981 tcgcgggcgc gttatatttc gggcgtgtca ctggtgttag gatggatgct cgccgtgatt
    20041 accgtgcttt ccttggtatt attccgttcg ccgctggtaa gtatgtacaa caatgatccg
    20101 gcggttttaa gcatcgccgc caccgtctta ctgttcgccg gcttgttcca accggcagac
    20161 ttcacccaat gtatcgcctc ctacgccttg cgcggctaca aagttacaaa ggtgccgatg
    20221 ttcatccacg ccgccgcctt ttggggctgc ggtctgctgc cgggctacct gctcgcctac
    20281 cgtttcgata tgggcattta cggcttctgg acggcattga ttgcctcgct caccatcgcc
    20341 gccatcgcct tggtgtggtg cttggaattg tgcagtaggg agatggtcag atcgcataag
    20401 gctgtctgaa aactccgccg attaaaatat gaaactatgg gctgaagccc gccactgaca
    20461 atacccattt taaagttcaa accatgcaac ccatccgata ccgaaccgac ctcaccccct
    20521 acaacacctt cggtcttcgc gcccaagccc gagcctttat tgcgcttgaa cacgccgacg
    20581 ggttgtgcga catcgtccga ctgcccgagt tcgaccgcga tactgtttta tggctgggcg
    20641 gcggcagcaa catccttttg atgcaggatt acgccggact ggtcgtacac atggaaaaca
    20701 aaggcatacg cgagattgcg cgttcagacg gcatggttct gattgaagcg caggcgggcg
    20761 aaatttggca cgattttgtc ctgcacaccg ttgcgctggg tttgagcggt ttggaaaacc
    20821 tgagcctgat tccgggtacg gtcggcgcat cgcccgtgca gaacatcggc gcatacggcg
    20881 tggaggcgaa agacgtgatt cacagcgtgc gctgctttga tttggatacg gagacctttg
    20941 tcgagcttgc caatgccgac tgccgcttcg cctaccgcga aagcctgttc aagcaggagg
    21001 gtaaagggcg ttatgtgatt gtttcggtcg tatttgcatt aaaaacgcat tttgtgccga
    21061 acttgggtta cggcgatttg gcggccgccg ttgccgaact gagcgcgggc agggtgccga
    21121 cggcgaaaga tgtttccgat gcagtgtgtg caatccgcaa cagtaaactt cctaatccta
    21181 acgtgcttgg taatgtcggc agtttcttta aaaatcccgt catcggcgcg gaaaaagctg
    21241 ccgccctgtt ggagcagcat cccgatatgc cgcattatcc gcagccggac ggttcggtca
    21301 aactcgccgc cggctggcta atcgagcaat gtcgtctgaa aggcttccaa atcggcggcg
    21361 cggcggtaca tgacaggcag gctttggtct tagtgaacaa aaacaacgcc tcggcaaacg
    21421 atgtccggca gttggcgcaa cacatcaaat ttacagtatt tgctcggttt caggtagaat
    21481 tgcacgccga acccaattgg ctgcctgctt cgttcagcct gtaaatccaa ggagtatgcc
    21541 cgtgacccgc cccgccaaaa tcaatactta cacacgcatc atcgacgcca gccttgcgct
    21601 tttcaacgag gaaggcgagc gcaacatcag caccaaccat attgccgccc acttgggcat
    21661 cagtccgggc aatctctatt accacttccg caacaaagac gaaatcatcg tccaactgtt
    21721 caaacgttac agcgaagccc tgctggaata cctcaacgaa gccgtgttgc cgtctgatgt
    21781 ggaagactcc atcaattata tggcgggcat ttacgacgtg atgtgggaat accgcttcct
    21841 ctttagcgat gtaaacaccc tgcttgcccg cagtgccgaa ttattgggcg aacacaacac
    21901 cttcacccaa gccaaagtct ccccgctctt ggtcaacctg ctgacccagc tcaacggtct
    21961 gaacatcatc caagccgacc aaaccgccat gaacgatctc gccgtcaata tgtggatggt
    22021 cacgaaatac tggttcgact tcgacagctc cctgcgcggc cgcaccaagc tgaccgaaga
    22081 ctccaaagca cgcggcatcc gccgcacctt aagcctcctt cgtccttatc ttttgcccga
    22141 acaccgcaag aaatacgacc ggaaaatcgg caacggcaac ccgtaaaccc acagcgtccg
    22201 aatcagcctt cagacggcac ttacccgacg gtatcagcga atgcgcgcag tccccccgcc
    22261 ccatatctcc atcacaggcc taggttacct cggcctgccg ctggcacaaa agttttacca
    22321 acacggcagc cgcgttgccg ccgtcaaacg cagcctgact tcggacgata tcaatctgcc
    22381 catacacctc gataccatcg acctcaatca agacagcgcg tttcaaagcg cgaaccttgc
    22441 ccgagataca agcttttggc ggcaccatgc caacaaaccc gtttggttct gcctcttgcc
    22501 gccatcatcg ctgacacatt acgccgatac cgtcaaacaa tgggcagaac ttgcccgggc
    22561 gtgcaacgtg caacacctga ttttcacaag cagtaccagc gtttacggcg atacagcgcg
    22621 cgaatgcgac gaaatcgccc tacccgatcc gcaaaccgag tccgcccgcc aaatcctcgc
    22681 cgccgaacaa cacctgctcg acagcggcgt tccgaacatc gacatcctgc ggctgggcgg
    22741 gctttattgc gccgaacgcc atcccgtcgg ccgccttgtt caaaagcaaa acatcccggg
    22801 cggcaaccgc cccatcaaca tcgtccaccg taatatcgcc gtcgaaagcc tgtttcagac
    22861 ggcatttaac cccggcggca ggcggctgaa aaacattatc gaaccgcgcc acccgacacg
    22921 acgcgaattc tatacggaag aagccgccaa actcggcttg cccgcgccgg attttgcacc
    22981 cgacgacagc gtgggcaaaa tcatccgtac cgtttgcgat aacggcttaa gcctgtaaaa
    23041 taagcggcaa cagcaacaaa caggcattct ccgccccgat cagaaaatat gtccgccctc
    23101 ctccccatca tcaaccgcct gattctgcaa agcccggaca gccgctcgga acttgccgcc
    23161 tttgcaggca aaacactgac cctgaacatt gccgggctga aactggcggg acgcatcacg
    23221 gaagacggtt tgctctcggc gggaaacggc tttgcagaca ccgaaattac cttccgcaac
    23281 agcgcggtac agaaaatcct ccaaggaggc gaacccgggg cgggcgacat cgggctcgaa
    23341 ggcgacctca tcctcggcat cgcggtactg tccctgctcg gcagcctgcg ttcccgcgca
    23401 tcggacgaat tggcacggat tttcggcacg caggcagaca tcggcagccg tgccgccgac
    23461 atcggacacg gcatcaaaca aatcggcagg aacatcgccg aacaaatcgg cggattttcc
    23521 cgcgaatccg agtccgcaaa catcggcaac gaagcccttg ccgactgcct cgacgaaata
    23581 agcagactgc gcgacggcgt ggaacgcctc aacgaacgcc tcgaccggct cgaacgcgac
    23641 atttggatag actaaccttc agacggcatc cgacatgaac agcccttttc acaacatcgg
    23701 catcgtaacc cgccccaaca cgcccgacat ccaagacacc gcacacacgc tgattacctt
    23761 tttgaagcag cacggcttta ccgtctatct cgacgaagtc ggcataaagg aaggctgcat
    23821 ctatacccaa gacaccgtcg gctgccatat cgtcaacaag accgaactgg ggcaatactg
    23881 cgacctggtc gccgttttag gcggagacgg cacctttctc tccgtcgccc gcgaaatcgc
    23941 cctgcgcgcc gttccgatta tcggcatcaa ccaagggcat ttgggcttcc tgacccaaat
    24001 tccccgcgaa tatatgacgg acaagctatt gcccgtttta gaagggaaat accttgccga
    24061 agagcgcatc ctgattgagg ccgcactcat ccgcgaaggc aaaaccgccg aacgcgccat
    24121 cgccctcaac gatgccgtcc tctcccgtgg cggtgccgga cagatgattg agtttgaagt
    24181 cttcgtcaat cgggaattcg tctataccca gcgttcggac gggctgattg tctccacccc
    24241 caccggatcg accgcctatt cgcttgccgc cggcggcccc atcatgcagg caggattaca
    24301 cgccttcacg ctcgtcccca tctgcccaca atccatgacc aaccgcccca tcgccattcc
    24361 agacacgtcc gaaatcgaaa tcctcgttac ccaaggcggc gacgcgcgcg tccatttcga
    24421 cggtcaaacc catatcgacg tgcaaaacct cgaccgcatc accatccgcc gctaccgaaa
    24481 tcccttacgc attttgcacc cgaccgacta ccaatatttc aaaaccctgc gccaaaaact
    24541 gcactggggc gagcaattag tctaagccgg cctttacccg aaagtatcca tgaatcccaa
    24601 aatttcctcc gaccatacag aagatgccgt gcgcctctcc aaacgcatgg cgcaactggg
    24661 gctttgttca cgccgcgaag ccgacggcta tatcgaacag ggttgggtaa cggtcaacgg
    24721 caaaaccgcc gtactcggtc agaaagtttc accggcagac cgtatcgaac tgaacaagaa
    24781 agcccacgaa cagcaggcgg cacgcattac catcctgttg aacaaacccg tcggctatgt
    24841 cagcgcacaa gcggaaaaag gctataaatc cgccgccgaa ctgattaccc ctgaaaatca
    24901 ctgggaaggc gataccggcc gcatccgttt cgatccgaaa cacaaaatcg gcctcgcccc
    24961 cgccggcagg ctggacatcg actcggtcgg attgctggta ttgactcagg acggccgtat
    25021 cgccaagcag cttatcggcg aaaacagcgg cagtgaaaaa gaatatttgg tgcgcgtgcg
    25081 cggcaaattg gacgaaaaag gacttgcctt actgaatcac ggattgagtt tggacggcga
    25141 gaaactgcgt cccgcccaag tagaatggca aaacgaagac caactgcgct tcgtgttgaa
    25201 acagggtaaa aagcggcaaa tccgccgtat gtgcgaactg gtcggactgc gcgtcgtcgg
    25261 gctgaaacgc atccgcatgg gcaaggtcaa actcggcagg ctgccgcccg gcaaatggcg
    25321 ttatctcgct cccggcgaat cgttttaaat aa
//
LOCUS       DAWHRA010000013        23529 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWHRA010000013 DAWHRA010000000
VERSION     DAWHRA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23529
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.013"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..143)
                     /locus_tag="WIB30_000388"
     CDS             complement(<1..143)
                     /locus_tag="WIB30_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796522.1"
                     /translation="MPSERLSDGIFPCVLNRVHETPDAFGGNVGCRAMFGGDQRTKCA
                     GKGL"
     gene            complement(161..718)
                     /locus_tag="WIB30_000389"
     CDS             complement(161..718)
                     /locus_tag="WIB30_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ3796523.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(794..2428)
                     /locus_tag="WIB30_000390"
     CDS             complement(794..2428)
                     /locus_tag="WIB30_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ3796524.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESAVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2709..3815)
                     /gene="serC"
                     /locus_tag="WIB30_000391"
     CDS             complement(2709..3815)
                     /gene="serC"
                     /locus_tag="WIB30_000391"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ3796525.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGATVVIIRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTEDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4180..4611
                     /gene="rimP"
                     /locus_tag="WIB30_000392"
     CDS             4180..4611
                     /gene="rimP"
                     /locus_tag="WIB30_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ3796526.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTISFDGKTVQIELGNIDKARLRPEFKF"
     gene            4639..6141
                     /gene="nusA"
                     /locus_tag="WIB30_000393"
     CDS             4639..6141
                     /gene="nusA"
                     /locus_tag="WIB30_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ3796527.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDYLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6153..9041
                     /gene="infB"
                     /locus_tag="WIB30_000394"
     CDS             6153..9041
                     /gene="infB"
                     /locus_tag="WIB30_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ3796528.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAATRAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAGKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
     gene            complement(9253..10647)
                     /locus_tag="WIB30_000395"
     CDS             complement(9253..10647)
                     /locus_tag="WIB30_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ3796529.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLETHTDTRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTYRPETSRTERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(10709..12058)
                     /locus_tag="WIB30_000396"
     CDS             complement(10709..12058)
                     /locus_tag="WIB30_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ3796530.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVVAATFAFFAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAMLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVVEGRLNGNIADARAWSGLLVGSIACYGILPRFLAWAVCKILLKTSK
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKTNDRT"
     gene            12201..12827
                     /locus_tag="WIB30_000397"
     CDS             12201..12827
                     /locus_tag="WIB30_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ3796531.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12891..14309)
                     /locus_tag="WIB30_000398"
     CDS             complement(12891..14309)
                     /locus_tag="WIB30_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ3796532.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            14636..15364
                     /locus_tag="WIB30_000399"
     CDS             14636..15364
                     /locus_tag="WIB30_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ3796533.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15423..15911)
                     /locus_tag="WIB30_000400"
     CDS             complement(15423..15911)
                     /locus_tag="WIB30_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ3796534.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRGRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSVAYFLAVALTVWWAWAR
                     AK"
     gene            complement(16669..17133)
                     /locus_tag="WIB30_000401"
     CDS             complement(16669..17133)
                     /locus_tag="WIB30_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ3796535.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            17458..18516
                     /gene="alr"
                     /locus_tag="WIB30_000402"
     CDS             17458..18516
                     /gene="alr"
                     /locus_tag="WIB30_000402"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ3796536.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(18580..19935)
                     /locus_tag="WIB30_000403"
     CDS             complement(18580..19935)
                     /locus_tag="WIB30_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ3796537.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAASKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(19950..20222)
                     /locus_tag="WIB30_000404"
     CDS             complement(19950..20222)
                     /locus_tag="WIB30_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ3796538.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(20348..21085)
                     /locus_tag="WIB30_000405"
     CDS             complement(20348..21085)
                     /locus_tag="WIB30_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ3796539.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(21366..22265)
                     /gene="prmB"
                     /locus_tag="WIB30_000406"
     CDS             complement(21366..22265)
                     /gene="prmB"
                     /locus_tag="WIB30_000406"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ3796540.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            22410..23003
                     /locus_tag="WIB30_000407"
     CDS             22410..23003
                     /locus_tag="WIB30_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796541.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHATSKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            23115..>23529
                     /locus_tag="WIB30_000408"
     CDS             23115..>23529
                     /locus_tag="WIB30_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ3796542.1"
                     /translation="MTKLYAEIAKMETQDDGTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFDAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANPDAV"
BASE COUNT         6211 a         6385 c         5818 g         5115 t
ORIGIN      
        1 agacctttgc ctgcgcactt ggtgcgctgg tcgcctccga acatggcgcg acacccgaca
       61 tttccgccga acgcatcggg cgtttcatga acccggttta aaacgcatgg aaaaatgccg
      121 tctgaaagcc tttcagacgg cattgtgctt gagattccgt ttaccaatgg ctgacaaacg
      181 cttccaaatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg accatcatca
      241 gcccgatgat tttatcctta tccgcacatc cgaaagcctc acgcaacaag gggctgttga
      301 cccacatccc cgtaatccag acattgtcga aaccttgcgc cgttgccgcc agttgcagcg
      361 catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc ttaggcacat
      421 cgcggttcgg cgcaaacgtt accccgataa ccatcggcgc catattgccc actttttcag
      481 cctttttcat cgcatcgtcg ccgaaattca attcggcaac cgtttgcttc aacacatcgc
      541 gaaaacgttg caatcctacc tcgccttgaa tcacggtaaa acggaagggg cgcatattgc
      601 cgtgatcggg aacttgggtt gccgcctgaa atatttgttc caactccgcc gcatcggggg
      661 cggggtgctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa gcatccatat
      721 cgttatactc cggtcatcgg tcgggtgttc ggacacatat gccgtccgaa ggcttcagac
      781 ggcatatccg gcatcagcgc ggacggcggc aggctgccaa tatatccatt tccttccgat
      841 aggtttggct gttggaaatg tccatcagcc ccaataccgt gctgaaatag tggtcgtgcg
      901 aatattcgtt ttccgccgct ttttgtttga ggcattggaa atctatgccc ccgtgttggc
      961 ggaaggcttt ggaaaaccac ataaccatcg ggatatgcgt ctgcccggaa ggcgcgatgg
     1021 cgtaaggcgc ggcgtgcagg tacatcccgt tttcgcccaa actttcgccg tggtcggaaa
     1081 cataatgcac cgcgctttcc aaatcgtcgc ggttttcaag tttgcggata accttgtcga
     1141 taaactggtc cacatacaaa accgtattgt cgtaagtgtt gaccagcgtg gcgcgggtgc
     1201 atttgttgat ttcgttggtg tcgcaggtcg gcgtgaattt gcgttcggct tcggtatagc
     1261 gttcgtaata cgtcggcccg tggctgccga tggtatgcag gattaaaacc gcgtctttat
     1321 cgtttttgtt gaggacttcg tcgaacttag tcagcaggat attgtcgaga cactcgccgt
     1381 tgcggcagta ttcgggcagg ttgagcgagg taacgtcggt attcggcact ttgccgcaca
     1441 cgcccttgca gccggaatcg ttttccaacc aggtaacttc cacgccggcg cgctgcacga
     1501 tgtccagcag gttgtcttgg tgttcggctt tgatttcgtc ataatccgtg cggtcgaagg
     1561 ttgagaacat acacggcagg gagtgcgcgg tcgatgtgcc gcagcttctg acctgcggga
     1621 aattgacaat ttcatcgccg cgcgcggcaa gcagcggcgt agtttggcgg ctgtaaccgt
     1681 tcaaacccca gttggcggca cgcgtggtct cgcccacgac cagcaccacg aaacggcgca
     1741 ggctgccggc cggccggttt tgcacgaccg ccatatccaa ttgcgtataa ggaatattgg
     1801 aacgcttcca atctttgtat ttcgacacgc ccgcgccgat gaaattagac ggcacaatca
     1861 gatgggttac tgatttattg ttgcggaaaa acgaggcgta atcctgatat tgcaacattg
     1921 cgatgcccaa cgcgcacaaa aaggaaacgg cggcaagcac aaggcgcgtc aaaagctcct
     1981 tataccaaac gcggtattta accttgacgg cgatatacgc cagcgcgggc aatacgccca
     2041 aacatacaat ccacagcaca tagcccggcg taatcaggcg cgcgctttcg gcagccgtag
     2101 tttgcaagac attattcaac atcgacttgt tgaaatagat attgaaaaat atttcttggt
     2161 aagacaccgc cgcactgata accaatatca acggaatcaa taccttatgc acgaaaggca
     2221 gggcaatgac gtgaaaaacg aaattactta aaaaaaacag caccaccggc atcgtataga
     2281 ggaagatatc cgccccggtg ccgttaaaag gatgaagctc gacaactttg gcaaaaaagg
     2341 cgtaattcaa taccagcgag gaatacaggg aaaggaaggc aatcagcgcg gaagagccga
     2401 gcttcggcct caggttcggt tttatcattt ggaatgtgtc ggataagggt tggaaaaggc
     2461 atccggcatt tggaatccgg attattgaaa aagattctta attataaggc aacggagcaa
     2521 agcagggcaa gaaaacggaa ggcgacgcag gctgtcggat acacatctcg gcacaaacag
     2581 catcacgccc ggattgaaaa ggacggatag ccgccggcag cacagtttta ccccgcatcc
     2641 caaaacgccg ccggagaacg ccgaaacagc cgatgccgtc tgaagccgct tcagacaaca
     2701 tcgggacatc aaccgtaacg ccgttggaaa tcgcgcataa aatctgccaa agcccgcacg
     2761 ccttcgagcg gcatcgcatt ataaatgctg gcacgcatac cgccgacggt tttatagccc
     2821 ttaagcaggc acaagccctg caattcggct tccagcacaa aacggcggtc aagctcctca
     2881 tcctccgttt ggaacacgac attcatttta gaacgcgcat tcggacggat acggttgata
     2941 tagaaaccgc cgctgccatc tatcgtctca tacaaggttt gcgccttcag ccgattgacc
     3001 gcttcaattt ttttcacacc gccctgcgcc tgcagccagc ggaacaccag ccccgacata
     3061 taaatcgcgt aagttgacgg cgtgttgtac ataccgtcgc ggttgatgtg cgaacggtag
     3121 ttgaacacat cgggaatatc gttcggacaa cgctcgagca aatcctcgcg gataatgacc
     3181 acggtcgcac cggccgggcc gatgtttttc tgtgcgcctg cgtaaatcag tccgtagtcg
     3241 gcaacatcaa actcgcgcga caaaatctcg ctggacatat cgcacaccag cggcggcatg
     3301 ccttctgaaa ggcgcggcac ttcacggtat tgcagcccgt tgaccgtttc attgacggca
     3361 aaatggacaa acgccgaatc gggtgcaaca tcccacgttt ccacaggcgg caggtcgaga
     3421 tagtcgaact gctcgccgcc atgcgccgcc aaacggattt ccgtatcggt caaacggctc
     3481 atctgttcat aagcgatacg gctccagttg cccgttacca ccgcgtcggc agtgcggaaa
     3541 ccgtgtgcca gattcatggc tgccatatta aattgggttg ttgctccgcc ctgcagaaac
     3601 aatatcttat agttgtcagg cactttcaaa agctgcctca aatcctgttc cgcatgatgc
     3661 aggatgctca aaaacatttc cgaacggtgg ctcatcgcca tcacgggaaa acccgtaccg
     3721 ttgtagtcca acatttcctg ccgcgccgtt tccaacacgg cttcgggcaa tacggcaggg
     3781 ccggcggaaa aattgtaaat cggataaaga gacatgatgc agccttgatt ctgaacaata
     3841 acccgcctga ttttaggctt gcagcaggct ttgtgcaagg atgggaaata cctgtcctcc
     3901 gccccgcttc atgccgcccg aacacggaaa acaacatcaa tatattgatt tacaaatata
     3961 aaaatcatgc tagcgacaaa tagatacatt tgttttgtca acaatattca cgatttccca
     4021 ttacaaacct cccttacacc cgcttttttc cgtcccaaaa acacaaaata aatcaacact
     4081 ttcatttctc cgcaaaagcg gttataatca cgccgatttt tcaactttga cgaaaaatgg
     4141 ccacggggcc atttttttat tatgtacata gggagcagca tggatatcca aaccatcctc
     4201 gaaaaaaccc tgcccggcct gggctacgaa ctggtcgatt tcgaactgac cgcgcaagga
     4261 acattgcgcg tgttcatcga caaagaaagc ggcattaccg tcgaagactg cgcaaccgtc
     4321 agcaaccact tgagccgcgt cttcatggtt gaagacatcg actacaaaaa cctggaaatt
     4381 tccagccccg gactcgaccg ccccttgaaa aaagccgccg acttcgtgcg ctttgccggt
     4441 cagaatgcca aaatcaaaac ccgcctgccg atagacggtc agaaaaactt tatcggtaaa
     4501 atcgaaggct gcgaaaatga taccgttacc atatccttcg acggcaaaac cgtacaaatc
     4561 gaattgggca acatcgacaa agcccgtctg cgccccgaat tcaaattcta aaacacagca
     4621 atattggaga tgttcaaaat gagtcgtgaa atgttacagc tggcagaagc actggcaagc
     4681 gaaaaaaacg ttgatgcgga agtcgtcttc caagcactgg aattcgccct gtctaccgcc
     4741 gccaagaaaa aggcagaccg cgaacacatg gatgtccgcg tacaaatcaa ccgcgacacc
     4801 ggcgaatacc aaaccttccg ccgctggctg attgtcgccg atgaagacta tacctatccc
     4861 gatgtcgaaa aaaccatcga ggaaatccaa gaggaaattc ccggcactac catccaaatc
     4921 ggcgaatact acgaagagca gctgcccaac gaaggcttcg gccgccaagc cgcgcaaacc
     4981 gccaaacaaa tcatcctgca acgcatccgc gatgccgagc gcgagcaaaa tctgaacgag
     5041 tttctcgccg tcaaagaaga catcgtgtcc ggcacggtca aacgcgtcga acgccacggc
     5101 atcatcgtcg aagtcgttgc cggcaaactg gacgcgctga ttccgcgcga ccaaatgatt
     5161 ccgcgcgaaa acttccgcag cggcgaccgc atccgcgccc tcttcctgcg cgtcgaagaa
     5221 atcggcaaca ccggccgcaa acaagtcatc ctgagccgta cttccggcga tttcctcgtc
     5281 aaactgtacg ccaatgaagt acctgaaatt gcagacggca tgcttgaaat ccgcgctgtc
     5341 gcccgcgacc cgggacaacg tgccaaagtc gccgtcaaag ccaacgacca gcgcatcgat
     5401 ccgcaaggca cctgtatcgg cgttcgcggt tcgcgcgtca acgcggtcag caacgaattg
     5461 tccggcgaac gcatcgacgt tgtcctttgg tcgcccgagc ctgcccaatt cgtcatgagc
     5521 gcgctctcac ccgccgaggt cagccgcatc gtcatcgacg aagacaaaca cgccgtcgat
     5581 gtcatcgttg ccgaagacca gctcgcgctc gccatcggac gcggcggtca aaacgtgcgc
     5641 cttgcttccg acctgactgg ctggcagctc aacatcatga cctctgcaga agcagacgag
     5701 cgcaatgcgg cagaagatgc cgccatccgc cgcctgttta tggattactt gaacgtggac
     5761 gaagaaaccg ccgacgtact ggttcaggaa ggttttgcaa ccttggaaga agtcgcctat
     5821 gttcctgccg ccgaactgct tgccattgaa ggatttgacg aagaaatcgt cgatatgctc
     5881 cgcaaccgcg cccgcgatgc catcctgacc atggcgattg ccgccgaaga aaaactgggc
     5941 gaagtatccg acgatatgcg caacctcgaa ggcatagatg ccgatatgct ccgcagcctt
     6001 gccgaagcag gcattaccac ccgcgacgac ttggcagagc ttgctgtgga cgaactgatt
     6061 gaaatcaccg gtgtaaacga agaaaccgca aaagccgtca tcctgaccgc acgcgaacac
     6121 tggtttaccg aagacaaata aagggggtac agatgagtaa cacaaccgta gaacaatttg
     6181 ccgccgagct gaaacgcccc gtcgaagacc tgttgaaaca gttgaaagaa gccggcgtca
     6241 gcaaaaacag cggcagcgat tccctgacgc tggacgacaa acagcttctg aacgcctacc
     6301 tgaccaagaa aaacggcagc aacggcggca ccatcagcat ccgccgcacc aaaaccgaag
     6361 tcagcaccgt tgacggcgta aaagtcgaaa cacgcaaacg cggacgcact gtcaacattc
     6421 cttctgccga agaattggca gcacaggtaa aagccgccca aacccaagcc gcacctgtcc
     6481 ggccggagca gacggcagaa gacgcggcaa aagcccgagc cgaagctgcc acacgtgcag
     6541 aagcccgtgc caaggcagaa gcggaagcgg caaaactgaa agcggcaaaa gcaggcaaca
     6601 aagccaaacc tgccgcgcag aaacccaccg aagcaaaagc cgaaaccgca cccgttgcgg
     6661 cggaaaccaa acccgccgaa gaaagcaaag cgggaaaagc ccaagccgac aaaatgccgt
     6721 ctaaaaaacc cgccgagccc aaagaaaaag ccgccaagcc gaaacacgag cgaaacggca
     6781 aaggcaaaga tgccaaaaaa ccggcgaaac ctgccgcacc tgccgtgccg caacccgtgg
     6841 tcagcgcgga agaacaggcg caacgcgatg aagaagcacg ccgtgccgcc gcacttcgcg
     6901 cccaccagga agccctgttg aaagagaaac aggaacgcca ggcacgccgc gaagccatga
     6961 aacaacaggc agaacaacag gcaaaagccg cacaggaagc caaaaccggc agacagcgtc
     7021 ccgccaaacc tgccgagaaa ccgcaggcag ccgcgccggc cgtcgaaaac aaacctgtca
     7081 atccggcaaa agcgaaaaaa gaagaccgcc gcaaccgcga tgacgaaggt caaggccgca
     7141 acgccaaagg caaaggcgga aaaggcggac gcgaccgcaa caatgcacgc aatggcgacg
     7201 acgagcgcgt acgcggcggc aaaaaaggca aaaaactcaa actcgagccg aaccaacacg
     7261 ccttccaagc accgaccgaa cctgtcgtac acgaagtttt ggtgcctgaa accattaccg
     7321 ttgccgactt ggcacacaaa atggcagtca aaggcgtgga agtggtcaaa gccctgatga
     7381 agatgggcat gatggttacc atcaaccaat ccatcgacca agacaccgcc ctgattgtcg
     7441 tcgaagaact cggacatatc ggtaaacctg ccgcagcgga cgaccccgaa gccttcttgg
     7501 acgagggcgc ggaagcagtg gaagccgaag cattgccgcg tccgcccgtc gttaccgtga
     7561 tgggccacgt cgaccacggc aaaacctcgc tgctggacta catccgccgt accaaagtgg
     7621 tacagggcga agcgggcggc attacgcagc acatcggcgc gtaccacgtt gaaacccctc
     7681 gcggcgtgat taccttcttg gacaccccgg gccacgaagc ctttaccgct atgcgcgcac
     7741 gcggtgcgaa agcaaccgac atcgtgattc ttgtggtcgc cgccgacgac ggcgtgatgc
     7801 cgcaaaccat cgaagcgatt gcccacgcca aagcggcagg cgtaccgatg gtggttgccg
     7861 tcaacaaaat cgataaagaa gccgccaacc cagagcgtat ccgccaagag ctgaccgcac
     7921 acgaagtcgt gcctgacgaa tggggcggcg atgtacagtt tatcgacgtt tccgctaaaa
     7981 aaggcctgaa catcgatgca ttgctcgaag cagtcttgct cgaagccgaa gttttggaac
     8041 tgactgcccc tgtcgatgca cctgccaaag gtatcatcgt tgaggcacgt ttggacaaag
     8101 gccgcggcgc ggttgccaca ttgctggttc aaagcggtac gctgaaaaaa ggcgatatgc
     8161 tgctggccgg tacggcattc ggcaaaatcc gcgcgatggt cgatgaaaac ggcaaatcca
     8221 ttaccgaagc cggcccgtcc atccccgtcg aaatcctcgg cttgtccgac gtaccgaatg
     8281 cgggtgaaga cgcgatggta ttggcggacg agaaaaaagc gcgcgaaatc gccctcttcc
     8341 gccaaggcaa ataccgcgac gtgcgccttg ccaaacagca ggcggcgaag ctggaaaata
     8401 tgttcaacaa tatgggcgaa acccaggccc aatccttgtc ggtcatcatc aaggcagacg
     8461 ttcagggttc ttacgaggct ttggcgggca gcctgaaaaa actgtccacc gacgaggtga
     8521 aagtgaacgt attacacagc ggcgtgggcg gcattaccga atcggatgtc aaccttgcca
     8581 tcgcgtccgg ggcgttcatt atcggcttta acgtgcgtgc agatgcctct tcgcgcaaac
     8641 ttgccgaaaa tgaaaatgtg gaaatccgct actacaacat catctatgat gccatcgacg
     8701 acgtgaaagc cgccatgagc ggtatgcttt ccccggaaga gaaagaacag gttaccggta
     8761 cggtcgaaat ccgtcaggtc atctccgttt ccaaagtcgg caatattgca ggctgtatgg
     8821 ttaccgatgg tgtggtcaaa cgcgattccc atgtccgcct catccgcaac aacgtggtta
     8881 tccataccgg cgagctggct tcgttgaaac gctataaaga cgacgtaaaa gaagtccgca
     8941 tgggctttga gtgcggtctg atgctcaaag gctacaacga gattatggaa ggcgaccaac
     9001 tggaatgctt cgacatcgtc gaagttgccc gcagcctgta attcctttgc aaataaaatg
     9061 ccgtctgaag ccttcagacg gcatcggaaa acgggttctg tatcatacag aacccgtttt
     9121 ttgtcgcaaa tcggcttcag cgaccctcct gccttatccc gatttgaatc tgacttgcca
     9181 tacaaacagg cttcagacgg cattatagtg aattaaattt aaaccggtac agcgttggct
     9241 cgccttgccg tactatttgt actgtctgcg gctcgccgcc ttgtcctgat ttaaatttaa
     9301 ttcactatat tttcccgaca ggcggatttg aagtttctcc acatatccgg cgcgttcggt
     9361 acggctggtt tcggggcggt acgtattgag cgacgaccat ttccaatgac tgcgggcttt
     9421 gttgagttcg ggcgggagtc tggcggcgtc ccacggggct ttgcggctgt gcagctcgat
     9481 atccgactgt gccgcgtgtc cgcgcgtttg caggacgtgg agcaaatcga gggcgcgggc
     9541 ggcgagcagg gtcaaggttt cagggtcggt gtgcaaggtt tggcggccgg agagtttgtc
     9601 ggaaatggtg cgggtattgg gcaggatgcc gcccaaaaag ccgccgattg ccgtacccaa
     9661 tccgagcgag ccgccgagtg tggcgatgtc cagccccaag ccgatgagcg cgccggttgc
     9721 cgcgcccgtg ccggtgcgga tgccgtattg tttgagcaat tcgctgtcga acgggtcttg
     9781 gcggaaggct tgcggcatcc agtcgccgcc gtcgatttcg ctgtggtaga aacggtagag
     9841 ggcaaacagc cgctgctgca tctgccgttc gagttggcgt atttccgcct gcatggtttg
     9901 cagcacggtg gcggtatcct cgttttcgtc cacttcctgc ctgaaggcgg cggcatcgag
     9961 caggaaatcg gcgatttcgc ggcgcgcttc gccgtccagc cgctgccatt cgcgccggcg
    10021 catggctgtc aggcggtcaa gtgtgctgcg ttcgggcaac atggtggcga ggttttccca
    10081 caggcgtagt tcgccttcaa aatcaaaggc gacggtgtcg aaccctgcga aaacgtgcag
    10141 gtttctcctc gccagcatgg ttgtccacga ttcgggaagc tgtccgccgg taaagttgaa
    10201 cacgggcata accggtttgg cacaccatga aaggatggtc aattcatccc gatatttgtc
    10261 gaggacgggt tcgcgcgcgt cgatgacgta cattgccata tcgctttgca agacttgccg
    10321 taagactttg gcttcctgat tgaaatcatg gtgcgcaccg tggctgccga gaaactgttg
    10381 cagccgctcg atgccgtctg aacgcgtatc cgtatgggtt tccagccatt ccaaaacacc
    10441 gcccgcgtct tcgagtccgg gcgtgtcata caggaaaacc agcatgtccg cgccgtcgct
    10501 gatggcggct tcttcgacat ggcgcgtagt ggacggggca tttttgactt cgccgaagcc
    10561 gctgtcgcgc aatagggtgc gcaggagcga ggttttgccg gtattggtat gcccgacgac
    10621 ggcgagggaa aggggttgtt tgttcatgat gtttttgaag aatggatttt cagacgacct
    10681 tttaaggatg gcggctttat ggggcgtttc aagtgcggtc gttggttttc agacggcctt
    10741 cctgcgccgc cctgtcaggc tcaagccacg ccgcgccgca ttcggtcagc gcgttacgcc
    10801 aatgttccag cttttccgaa aggtcgtctg aaagcccctg ttccgccaaa agctgcacca
    10861 ccgcgccgcc ctgcgccgct tccgaaagtc ggacgatctg ccgcaacacg ccgcggtcgg
    10921 gcacagtttg ggcgcgcacg ccgataagca gttgcgccgg tttctgcttc agctctgtct
    10981 ccagcgcggc aacctgttcc cgattggcgg caacgccctt atccagccat tcctgcgcca
    11041 gcctgccctc gaaccattcg ccgtcctgcc attcggtctc cagcatgacc gcccatttcg
    11101 gcgcatcgtt caagacgatt ttcggcgaaa cggcggacac ggtttcccga cgcgtatccg
    11161 catcggtgat tttgttctgc cagcggcgga tgaccgcctg ataatagggt ttttccaaat
    11221 ccaagccgtt tttgcttgtt ttcaaaagga ttttgcatac cgcccaagcc aagaagcgcg
    11281 gcaggatgcc gtagcaggcg atactgccga ccagcagccc cgaccaagcc cgcgcatcgg
    11341 caatattgcc gttcagacga ccttcaacta ccgcccgcgc atcggggaca gggaaaccga
    11401 gtttcgacgg cagccatgcc aacatttcca ccgcgcgtac cgaagcggca ttgctcaaca
    11461 gcgtgctttc ccagttgaac gtatattggc gcaccaaaag cagcaacaat accgacacca
    11521 gcattccgag cagcgtgcag agccacaggc tgtgcgacgt tgcgcctatt ttccaacgta
    11581 ccgaaggttg ccgccactcg tccgcataca gccgcaacat cgcctgattg acagggtctt
    11641 tgccccgaaa ccacgtcgcc ggactgctga aaaaacgccc cactttcacg cgcaggaaca
    11701 acattgccaa ccatactgcc agcatcagcg tattcatgcc caacacgccc gccaaaacca
    11761 aaaagaaatt cagcccctga ttgtccatta gaagataagt gcctgaaaaa ccggcaaaaa
    11821 acgcaaacgt cgccgccacc acccacaacc agaacgaccc cgcacgcaca cgttccaacg
    11881 tctcccgcag catacggttc ctgtcaatca tctccgcccg acggatgatt ttttcctccg
    11941 tactgccgtc cacgcggcgc aaagcctccg tcgcctgcac gggatcgccg ctgaaaataa
    12001 agccgccttc ttccaaaata cggaccagct caaccagttt tcgggatgga ttcaacataa
    12061 aatgccgtct gaaaataaaa aacagatttt aacacacgca ttttcaagaa tattcacagt
    12121 gtaggcaaag agtaaatccc acacagaggc aaaagtatcg gcgtaaactg actgcctcta
    12181 ctttcccgaa agattgtgcg atgtatacag gcgaacgctt caatacttac agccatttga
    12241 gcggtttgat tctggcggcg gcaggtttgg tgctgatgct gctgaaaacc ataggacacg
    12301 gggacggcta ccgtatcttc agcgtatcgg tttacggcat cagccttctt ctgctctatt
    12361 tgagttcctc gctgtaccac ggaattgcag ccggaaaact gaaaagcatt ttgaaaaaaa
    12421 ccgaccactg catgatttat gtcctgattg ccggaagcta cacaccgttt gcactggttt
    12481 ctttgagaaa cgggccgggc tggacggtat tttcactgtc ctggctgctg gcggctgcag
    12541 gaatcgcaca agaactcacc atcggacgga aaagcgaaaa acgtctgctg tctattgtga
    12601 tttatgtcgt catgggttgg atggtcttgg cggtaatgaa atccctgaca gcctcactcc
    12661 cgtcggcagg actggcttgg ctggcggcag gcggtatgct gtacagcgtc ggcatttact
    12721 ggtttgtaaa cgatgaaaaa atccgacacg ggcacggaat ctggcatctg ttcgtattgg
    12781 gcggcagcat cacccaattt gtcagcgtgt acggttacgt aatctgaatg ccgtctgaaa
    12841 agcaaaacct cccgatcctg aagattggga ggttttctgt ttgccggaca tcagcccttg
    12901 tcgtggaact cgtgaaattc atactgatag gacaaatccc gacccgcttt tttctgtgcc
    12961 aaataatcat cataaatggc acggatttcc ttacgcaaca aaaacagggc tatcaagttc
    13021 gggataacca taaagccgtt gaacatatcg gacaggctcc aaaccaaatc gactttgccg
    13081 agcgtaccca aaacgatggc aagcagaacc aatgcgcgat agatgcccaa gtgtcttccc
    13141 ctgaaaagaa aacggatgtt ggactcaccg aaataatacc agccgataat ggtcgtcaag
    13201 gcgaaaaagg tcagacacac ggcaagcaat tgcgaaccga agcccggaaa tgccttgtta
    13261 aaggcaaatt gagtaaccgc cgcgccctgt tcgcccgaaa ggttggcatc agtcagcagg
    13321 ataatcaatg ccgtagccgt acataccaaa atcgtatcga taaacacacc gacaaatgcc
    13381 gccatacctt gctgcacagg atgcttcaca tcagcggttg catgtgcatg cggggtagaa
    13441 cccatacctg cttcgttgga aaacagaccg cgcgccacgc cgaaacgtat cgcttcacgc
    13501 ataccgatac ccgcagcacc gcccaaaacg gcttcaggat tgaaggcggc ggtaaagatg
    13561 tggttgaaca tcggcacaat atggtcggaa aattcaaaca ggataacgac ggcgcacaaa
    13621 atataaacaa ccgccataaa cggcacgaca aactgggcaa tattggcaat acggttcacg
    13681 ccgccaatca caaccatgcc cgcaaggacg gcaagcacaa taccgactgc caaagaaggc
    13741 acatcaaatg caatggtaac ggcagaagca atggagtttg cctgtgtcgc attaccgata
    13801 aagcccaatg cgataatcaa cgcaatggag aaaaagcccg acaggaaacg cgccgcgccc
    13861 ctgccgattt tcggagtcag accgtgcgta atataaaacg ccggcccacc gatgtatttg
    13921 ccgtggctga cgacgcggta tttctgcgcc agcagtgcct ccgcaaaaat cgtggacatc
    13981 cccaaaacgg cagaaaccca catccaaaaa atcgcgcccg gcccgcctgc ggtgatggcg
    14041 gtcgccacgc cggcgacgtt gcccgtaccg atttgcgcag atatggcaac cgccaacgcc
    14101 tgaaactgcg ataaagactt gtcgtcttta tcgcctttgg caaacaagcc gccgaatacg
    14161 gatttgaatc ccgcgcccag cttggtaatc tgcggcgcac cgagatacag cgtaaaaaac
    14221 aggccgatac ccaaaagcgc gtaaatcagc aggtagtccc aaaggaaccg attgactgta
    14281 cccaccagaa cagacaatat attttccata aaataaacct tatcttacaa ttaaaatgac
    14341 tgccttccaa aagacattcc aataaggaaa cacggcgagc agaccgtatt tgccgcaaca
    14401 gatgccttaa attgtcaaca atcggggaga agctgcgcca tcataccgta aaatatcgtt
    14461 aattaaaaca tttctttatt tttaagcgga aagcggagga aatcgctttc agacggcatc
    14521 gacaacggca cggcataaaa caggatattt tgggtacttg caacttatgt taaaatgccg
    14581 accgtaaaaa atctgacaaa aacagattaa ttatttgaaa taagaaagga aatttatggc
    14641 aggccatagc aagtgggcga atatccagca taaaaaagcc cgtcaggatg ccaaacgcgg
    14701 caaaatcttc acccgtttaa tcaaagaaat caccgttgcg gcgcgtatgg gcggcggcga
    14761 tcccggcgca aatccgcgcc tgcgcctggc tttggaaaaa gcagccgaaa acaatatgcc
    14821 caaagacaat gtgcaacgcg ccatcgacaa aggtacgggc aacttggaag gcgtggaata
    14881 catcgaattg cgctacgaag gctacggcat cggcggcgcg gctttgatgg tggactgcct
    14941 gaccgacaac aaaacccgca ccgttgcgga cgtacgccac gcgtttacca aaaacggcgg
    15001 caacttgggt accgacggct gcgtggcgtt caacttcgtg catcagggct atttggtatt
    15061 cgaacccggc gttgacgaag acgcgctgat ggaagcggct ttggaagccg gtgcggaaga
    15121 cgtggttacc aacgacgacg gttccatcga agtcattacc gcgccaaacg attgggcggg
    15181 cgtaaaatcc gctttggagg cggcaggtta caaatccgtt gacggcgacg ttacgatgcg
    15241 cgcccaaaac gaaaccgaac tctccggcga cgatgccgtc aaaatgcaaa aactgattga
    15301 cgcactggaa gatttggacg acgtgcaaga cgtttacact tccgccgtat tgaatttgga
    15361 ctgatacgca gcacagcaga catacaataa aatgccgtct gaacctttca gacggcattg
    15421 atttatttag cccttgccca cgcccaccaa accgtcaggg caaccgccag aaaatacgcc
    15481 acactccaaa caggcaaagc aacccccagc agataatccg gttcggcaca atttccgaac
    15541 ccgcgcacga caggctcgaa ccaatcaaac aaaggccagc ccttcaatcg aaacgtccac
    15601 ggcgcaccgc acgaaggagc cgtacccggc ggcagcgact gcagccacaa ctgatatgcc
    15661 gcaacagaaa tacccgtaac ggcaggaatg ctgataaaga cagcaccgaa caaaccgcct
    15721 gcccttcctc ttggtctgca catcaggaca attgccgtac acaatgcggt tgccaaaacg
    15781 cataaccgct gactgataca caaaacgcaa ggctccatac ccaaaacata ctgtgccgcc
    15841 aaagaaccgg caaatgcaca gaccgaaacg gcaaacagca gccaaacggc ttttctaaat
    15901 aacggggtca ttttctcaac acaccaatca aaataccgat atgccgattt tgctggatat
    15961 atcccgagat ggcaagggac gaatcgacac cggcgcaggt gcgggcgatt tcggttttcc
    16021 cgatatgcac aaaacctgac aagggacaaa acggcgattt gcccgcacaa tgcccacaaa
    16081 aaaatacatc cggaggattt gaatttcagc aaatttagcg aatcaaaagt ttatttcaat
    16141 gaaatcatat gatttttttg aataagcgga ttgatgggtt tttgaaggaa tttgttaccg
    16201 gatagccatc gggcaagttt tttgcaaaat ttgaatcggt cgggtaaatt ttcaaaaaat
    16261 aattgacagc ggataagaaa cggcggataa ttcccgccgt cgagttgctt gatgcagctt
    16321 gatttttctc ctctatttct cctttgtaga cttggcacac attcaactgg atgtgtgcat
    16381 ttttttatct ccgctttttt tgaaatttaa ttacttttat tgtttggaat tccattcttt
    16441 aagaaattta acaagaaaaa cgcttgcttt ttgaccggaa aagccgcata attcattccg
    16501 ttagctggtt cgagtagtca gttaatagtt tctcctctat ttctcctttg tagacttggc
    16561 acacattcta ttgaatgtgt gcattttttt atctgaagca acaagcctct gtgcgtgatg
    16621 ttgttatgtt tcatttaggt gtcaaaccgc atatccggtc tgaaatattc aatccaaatc
    16681 caaaaccgga ttttctttga cctcctccat cacaacataa ctcctactct ccgaagcggc
    16741 aggcagttgc aataagatat tgcctagcat atcccgatag gcagacatat cgggcaaacg
    16801 tactttaatc agatagtcgt attcgcccga caccaagtgg cattccataa tttgcggaat
    16861 tttcagcact tcttttttga aatcttcgaa aatattgccc gatttggatt gcagcttcag
    16921 ctcgacaaaa accaatagcg gtttgcccaa cagatgggga ttgaggtggg cgtgatagcc
    16981 ggaaatataa tgttcccgct ccaaacggcg caccctctct gtaacgggcg tggtggacaa
    17041 gcctaccttc tcggcaagct ccgtcatcgg gatgcgggca ttctgttgga ggatcttaag
    17101 aatgcggaaa tcgattttat ctagttcttt catttggatt gccttgtatt tattattgat
    17161 tttaacaaat agagtatata gtggattaac aaaaaccagt acggcgttgc ctcgccttgc
    17221 cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
    17281 atatttggga aagcgattat atcaggaaaa gcaaaccgcc ttcctacctg aaaactgctg
    17341 cttcggcttg aagacacaag ggtctttaat attttaaaag ccttgccgtt ggattataat
    17401 tccctaattg atttcttaat tttgctaata aacacttgtt tggtaaggaa tgaatttatg
    17461 cgccctttga acgtgcagat caggttgggc aaccttaggc acaattatcg gattttaaag
    17521 gaaatgcacg gaggcaagct gttggcggta gtgaaggccg acgcatacgg acacggtgcg
    17581 gtcagatgtg ctttcgcgct ggcagacttg gcagacggct ttgccgtggc gacaatcgat
    17641 gaaggaatca ggctgcggga gagcggcatt acccatccga ttgtcctttt ggaaggcgta
    17701 tttgaagcat cagagtacga agcggtcgaa caatactcgc tctggccggc agtcggaaac
    17761 caatggcaac ttgaggcttt gctttcccgc cattggaaaa aacctgtcaa ggtctggttg
    17821 aaaatggatt cgggaatgca ccgtaccgga tttttccctc atgattacgc ttcggcatat
    17881 gcggcattga aacagtcgga atatgtggac agtattgtca aattctcgca tttctcctgt
    17941 gcggacgaac ccgaaagcgg tatgacggaa atacagatgg aagcattcga tttgggtacg
    18001 gaagggctgg aaggcgaaga aagccttgcc aattcggcgg ctattttgaa tgttcccgaa
    18061 gcacgcaggg attgggggcg cgcgggcttg gcgttatacg gcatttcccc gttcggaggc
    18121 agcgatgaca ggctgaaacc cgtgatgagg ctttcaaccc gtatttttgg cgaacgcgtt
    18181 ttacagccgc actcccctat cggttatggc gcaacatttt ataccagcaa atctacgcgc
    18241 gtcggcctga ttgcatgcgg ttatgcggac ggttatccgc gccgcgcccc aagcaattcc
    18301 cccgtcgccg tcgacggtaa attgacccgg gtcatcggca gggtctctat ggatatgatg
    18361 accatcgagc tggatgcttc gcaagaaggt ttgggacacg aggtcgaact gtggggcgat
    18421 acggtcaaca tcaatactgt tgccgaagcg gccggaacca tcccttacga attgatgtgc
    18481 aatatcaaac gtgcaaaatt cacttatatc gagtaatcga gtccaaacaa aaatgccgtc
    18541 tgaagccttt cagacggcat ttccccatca aaaccgcaat cagtttttca tcgattggac
    18601 cggagcggga attctgccgc cccgtttgac gaacacttcg cacgaacctt cttttaccgg
    18661 catcacaggt gcgtagccca acaggccgcc aaactcgacg ctgtcgccga cggttttacc
    18721 ggttaccgga ataatgcgca cggcagtggt tttactgtta atcataccga tggcggcttc
    18781 gtcggcgata atgccggaaa tggtgtgcgc gggtgtgtcg ccgggaacgg caatcatatc
    18841 caagccgacc gaacaaacgg cggtcatcgc ttcgagttta tccaacgtca gcacgcctgc
    18901 ttcggcggcg gcaatcatac cttcgtcttc ggaaacgggg ataaacgcgc cactcaaacc
    18961 gccaaccgcg ctcgaagcca tcatgccgcc ctttttcacg gcatcgttca gcaatgccaa
    19021 agctgctgtt gtgccgtgcg taccgcagac gctcaaaccc atttcttcaa gaatgcgcgc
    19081 caccgagtcg ccgacggcag gggtcggtgc cagcgacaag tcgagaatac caaacgggat
    19141 attcagcatt tttgaggctt cgcggccgat gagttcgccc acgcgggtaa ttttgaaagc
    19201 agttttcttc actacttccg caacttcggt caatgtcgtt gcatctgaat tttccaacgc
    19261 ggcttttacg acacctgggc cggatacgcc gacattgata acggcatccg cttcgcccga
    19321 accatgaaac gcgcccgcca taaacgggtt gtcttccacc gcattgcaga acacgacgat
    19381 tttcgcacag ccgaaacctt cgggcgtaat ttcagccgtg cgtttgatgg tttcgcctgc
    19441 cagcttgacc gcatccatat tgataccggc acgcgtactg ccgacattga tggagctgca
    19501 cacgatgtcg gtggtcttca tcgcttcggg aatggaacgg attaacacct cgtctgaagg
    19561 cgacatgcct ttttgcacca gcgcggaaaa gccgccgata aaggacacgc cgatggcttt
    19621 ggcagccttg tccaaagtct gcgccacgct gacgtaagaa tcggctttgc tcgccgccgc
    19681 gatttgggca atcggcgtga cagaaatgcg ctgattcaca atcggcactc catatttggc
    19741 agacagatat tttgccgtcg ccaccaagtc tttgccgacc gtggtgattt tgttgtaaat
    19801 gttttggttt aacacgtcga tgtcggtgct gatgcagtcg tgcaaatcaa tgccgatggt
    19861 aatggtgcgg acatcgaaat tctggtcggc aaccattttg acggtttcta aaatttcgcc
    19921 ggattggata ctcatcacat tcctccggct caaatgcggt gcatcgcttg gaagatttct
    19981 tcgttttgca tacggatgtc gagcgcgagt tttttgctct cttccgcaaa caaatccaaa
    20041 acctcctgcc gcgatttgct gcattttgaa gtgtccacca agataatcat ggtaaaaaaa
    20101 tcatccatca gctgttggct gatattgagg atgttgattc ggttttccgc caaaattttg
    20161 gaaacatcgt acacgatgcc gacgcggtct ttaccgatga cggtgatgac tgaattgttc
    20221 acaggcttac tccttgcaga tatccgttaa agtccgaaat tataccaccg ttggattttg
    20281 aagaaatatt gtcaacaata tatacataca aaatgccgtc tgaaactatt tcagacagca
    20341 tcaagattca gggttcgatt aaataaccat ccttatccca ctgggttttc ctgaccaact
    20401 tgtcatcctg ataaacagcc tcgctctttt tagaaccatc ttcataccac tccaaaacca
    20461 ccccgttgcg ttgatggtgg cggatagaca gttccgagag taatcggccg ctttcatccc
    20521 aagtcagaat tttggcaggc tcatcgttga ccataaccat ttccgtcttg atactgccgt
    20581 cggcatacca ttgcttccat acgccgtttg ccttattttg cttaaactgg atttcgcttt
    20641 ccttgccgcc gttacggtaa tagcggtacc ccgtaccttc actcaaacca tttttataag
    20701 gcataacggc agatttttta ccgttcggat accagttgac ccactccccg tccggcttac
    20761 ccttgctgaa gccccccgcc atttttttct gaccattaaa atgccacaaa atcaacatac
    20821 cgttttgcag ggtaggcaca aaagatttga tttgcgttga agcaacgata taaggttcgg
    20881 aatatttctt catcgacgga taataaaaat cctgcgcgtg cgcaataccc tccgccacac
    20941 tatattgcct gatataagcg gcagaagaca tcgtcgccgt cagtttcccg ttctgattaa
    21001 aataaacaga ataggtctgc gccggcaaag cggccgaaaa acccaacagg acagttgaaa
    21061 atacaatccg agataatttt ttcattgcaa tagcgatata aaaacaaggc tgtgttttag
    21121 taatcttttg atttcaatta tttgcaaggg aaaagacaat tattttccgg ttaggaataa
    21181 acctattcta ttgaatatat tgaagccaag tacgcctatc aacactatat taaaacactg
    21241 ccaaaaacaa ttaacttata aacaatatgg taaggatttc tctgccaagc atcaaacccg
    21301 agacaacgta tcgtaaaaat gccgtctgaa aacaaatcgt cttcagacgg catttcccct
    21361 tcaactcact cttcacccaa taactgctcg cgcgtcaaga ggaaaacaaa accgtcgccc
    21421 ccgctggttt ccaaccaagt aaaaggcaac tccggatacg ctgcttccaa tacatccctg
    21481 ttatgcccga tttccaccag caatacacct ttgggattca gaaactttgc cgcattcaga
    21541 agaatctgcc tggtggcatc caacccgtcc gccccgctgc ccaatgccaa ttccggttcg
    21601 tgcaaatact cttcaggcaa taactcaacc gattccgcat ccacataagg cggattggaa
    21661 acaatcaaat cataagtgcc ttccaatcct tcaaacaaat ccgtatgaat aagccggatg
    21721 cgttcttcca aaccataatc ttcgacatta atccctgcca cttccaaagc atccaagctc
    21781 acatcaaccg catcaatttg ggcatcagga taatgatgcg ccatctgaat ggcaaggcaa
    21841 ccgcttccgg tgcaaagatc caaagcatta tgcaccaact catcgtattc tatccaagga
    21901 cgaagtccgt cacccaacaa ttcataaata aaagaacgag gtatgattac gcgctcatcc
    21961 acatagaaat caaactctcc ctgccatgcc tggtgtgtca aataagcggc tggaatgtgt
    22021 tcgacagcac gacgctcaat aaccgtcagc acttcctctt tttcagcttc caagagtttt
    22081 gcatcaagat atggggcaag catatccaaa ggcaaattca aagtatgcag aatcaaataa
    22141 gctgcttcat catgcgcatt atccgttcca tgaccaaaaa agagccctgc ctcattaaaa
    22201 cggctgactg caaaacgtaa aacatcgcgg atagtcgtca attcttgtgc tgcctgatta
    22261 aacataatat gaaccattct gcgtatagat acttttaatt ataacagaaa caacaagcaa
    22321 accttttcat atcgccaaat aaccacccaa tctacccata caactacata aatgcccgcg
    22381 cgaaaaccat cgcccgaacg gaaacgacaa tggccgacgg tatgggcaac ctgattggct
    22441 gggaaaaaac ggggcttgtt gccggtaagc agtggataac cgcaaaagac gacaaggtgt
    22501 ccgatgtctg caatgccaac ggcgagatgg gcgtaatcgg gctttacgag cctttctcac
    22561 acggcgcatt gacgataccc ggtcatccga actgccgatg cgaggttgtt tccgtatcgg
    22621 gtggcgaatt gggggaattt gccgaaaaaa aggagcttcg taaagcggct atgcagtatg
    22681 cgcgggataa ctttatcggc aaaagctatg tcaatgaaaa cagcgggcat gaactgaagg
    22741 taacttggca aggtgtgaaa cacgctacgt caaaggcaaa tcaggcggaa ttatccatca
    22801 tgacaaaact tgatgactta ttgcgctacg caaaatatga gggttcttat tcggatagga
    22861 aaggtcatcc taatattatt gcagcacata agtatcgtgc cgttgccaag gttgggaatg
    22921 agtctttaaa tatcggtgtg attgtaaggg aatttccaga cgaccataaa cattacgacc
    22981 atttcatctt gaaggatgaa taaagccctt ttgcagtgtc gttctggagc ggatagcgtt
    23041 aaggcaagta cacttccagc cttgaaaaag ggctttaaat tcagcatgcc atttatacag
    23101 gcaggagtaa acccatgaca aagttatacg cagaaatcgc caagatggag acgcaggacg
    23161 acggcacggt caaggtttgg ggttacgctt caagcgagga gatcgattcg gacggcgaag
    23221 tcatcgcggc ggcagctatg aaggcggcga ttcccgatta tatgaagttt gacgcggggc
    23281 gcgagatgca cggctcaaac gctgcgggaa cggcaattga aatcaacgtt gaagacgacg
    23341 gcataacctt tttcggtgct catatcatcg accccgttgt cgtgtcgaaa gtcaaaacag
    23401 gcgtttacaa aggcttttcc atcggcggca gcgttaccgc ccgcgatgat ttgaacaagt
    23461 cgcaaatcac gggcttgaag ctgacggaaa tcagccttgt cgagcgccct gccaatcccg
    23521 atgcggtgt
//
LOCUS       DAWHRA010000014        22990 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWHRA010000014 DAWHRA010000000
VERSION     DAWHRA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22990
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.014"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            1..167
                     /locus_tag="WIB30_000409"
                     /pseudo
     CDS             1..167
                     /locus_tag="WIB30_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
     gene            complement(431..1345)
                     /locus_tag="WIB30_000410"
     CDS             complement(431..1345)
                     /locus_tag="WIB30_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ3796543.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPNVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1601..3070
                     /locus_tag="WIB30_000411"
     CDS             1601..3070
                     /locus_tag="WIB30_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ3796544.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3322..3693
                     /locus_tag="WIB30_000412"
     CDS             3322..3693
                     /locus_tag="WIB30_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796545.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3766..5175
                     /locus_tag="WIB30_000413"
     CDS             3766..5175
                     /locus_tag="WIB30_000413"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ3796546.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGQTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDTTIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            5221..6015
                     /locus_tag="WIB30_000414"
     CDS             5221..6015
                     /locus_tag="WIB30_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796547.1"
                     /translation="MTLPMQETCFSILLDELAAKQETAIASHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLAGIPLDAQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDE
                     ENISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSEQP
                     AEIQRLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADIPP"
     gene            6203..6979
                     /locus_tag="WIB30_000415"
     CDS             6203..6979
                     /locus_tag="WIB30_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ3796548.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            7055..7161
                     /locus_tag="WIB30_000416"
                     /pseudo
     CDS             7055..7161
                     /locus_tag="WIB30_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7232..7375)
                     /locus_tag="WIB30_000417"
     CDS             complement(7232..7375)
                     /locus_tag="WIB30_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796549.1"
                     /translation="MENNKVKSDYAAKQESLGKVDENKNETMKIPPQVGTHMLGFSFI
                     FKC"
     gene            complement(8052..10523)
                     /locus_tag="WIB30_000418"
     CDS             complement(8052..10523)
                     /locus_tag="WIB30_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ3796550.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVREAAVVKLQ
                     AGDVVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRVSSGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LVVLGNAMRLHKQVDMPSEKMPSEQ"
     gene            complement(10520..11698)
                     /gene="hflX"
                     /locus_tag="WIB30_000419"
     CDS             complement(10520..11698)
                     /gene="hflX"
                     /locus_tag="WIB30_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ3796551.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11703..12983)
                     /locus_tag="WIB30_000420"
     CDS             complement(11703..12983)
                     /locus_tag="WIB30_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ3796552.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(13136..13861)
                     /locus_tag="WIB30_000421"
     CDS             complement(13136..13861)
                     /locus_tag="WIB30_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ3796553.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13934..14611)
                     /gene="radC"
                     /locus_tag="WIB30_000422"
     CDS             complement(13934..14611)
                     /gene="radC"
                     /locus_tag="WIB30_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ3796554.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14734..16083)
                     /gene="gshA"
                     /locus_tag="WIB30_000423"
     CDS             complement(14734..16083)
                     /gene="gshA"
                     /locus_tag="WIB30_000423"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ3796555.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFSKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            16343..17752
                     /gene="leuC"
                     /locus_tag="WIB30_000424"
     CDS             16343..17752
                     /gene="leuC"
                     /locus_tag="WIB30_000424"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ3796556.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17849..18103
                     /locus_tag="WIB30_000425"
     CDS             17849..18103
                     /locus_tag="WIB30_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796557.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            18165..18806
                     /gene="leuD"
                     /locus_tag="WIB30_000426"
     CDS             18165..18806
                     /gene="leuD"
                     /locus_tag="WIB30_000426"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ3796558.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEERVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18981..19241
                     /gene="dcm"
                     /locus_tag="WIB30_000427"
     CDS             18981..19241
                     /gene="dcm"
                     /locus_tag="WIB30_000427"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ3796559.1"
                     /translation="MQQIKFIDLFSEMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFA"
     gene            19287..19802
                     /gene="dcm"
                     /locus_tag="WIB30_000428"
     CDS             19287..19802
                     /gene="dcm"
                     /locus_tag="WIB30_000428"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ3796560.1"
                     /translation="MARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETL
                     EALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPT
                     ESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKE
                     RRKKNGLQKSA"
     gene            19817..20251
                     /locus_tag="WIB30_000429"
     CDS             19817..20251
                     /locus_tag="WIB30_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ3796561.1"
                     /translation="MPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDQNDVAPTLVAMNMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            20267..20428
                     /locus_tag="WIB30_000430"
     CDS             20267..20428
                     /locus_tag="WIB30_000430"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ3796562.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            20429..20998
                     /locus_tag="WIB30_000431"
     CDS             20429..20998
                     /locus_tag="WIB30_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ3796563.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPG
                     FDIADFKMYSDKIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRLGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKK
                     KFEEAYKKHYNRSISIPRWHEIAHKYKKK"
     gene            21032..22102
                     /gene="leuB"
                     /locus_tag="WIB30_000432"
     CDS             21032..22102
                     /gene="leuB"
                     /locus_tag="WIB30_000432"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ3796564.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLNILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            22265..22828
                     /locus_tag="WIB30_000433"
     CDS             22265..22828
                     /locus_tag="WIB30_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ3796565.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5616 a         5983 c         5919 g         5472 t
ORIGIN      
        1 gctttgaaaa aacaataccc caatgcttcg aaagaactat acagggtggg ggttcaatcc
       61 acgactggaa cagtacgcat ttcgccaaac ggcaaaatcg cattaacgga attgaaaact
      121 tttggaattg tgcaaaacgg tatttgcgtc agtttatagt ggattaacaa aaaccagtac
      181 ggcgttgcct cgccttagct ccaagagaac gattctctaa ggtgctgaag caccaagtga
      241 atcggttccg tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
      301 tccactataa cggcatttcc aaagggcatt ttaagccgta tttgagagaa tgcggatggc
      361 gttttcagtg ttttcagcgt gataaaattc aaatttctat tttaaaacaa tcagcaaagc
      421 aggatttatt tcagttgtcc gagacaacgg gcggctgcac cgcctgcact ccccatactt
      481 tttcaatgca ctcgagcagg agtgagacgt tgggattatc ctcgtattct tccgcataaa
      541 tgaaattgag cggcatactg tggcggcacg attcgataat agccacgccc gcgccttctt
      601 ttgccgccgc ttcggctttg tttcccggca ccattgccac acctataccg cctgcaacca
      661 aatcgataat ggtttggggg tagtcgcaca agatctgttt tttgggtgag agccggttgc
      721 tgcgccaaaa ctggtgcagg tgcttcctac ttccggacac gcccgacatt tctatccata
      781 cgcattcttg caggctctgc ggaagggagc gggtcagatg gggatattgg ctttgcgggc
      841 aaatcagcgc gtaggtcagg ttttgcagga atacgctgcg gatgccgcgt tgggcggcgt
      901 tgccgagtat aaagccgccg tgcagggttt tgtgttggat gcgcgagagg atttcgccgc
      961 tcattccgta ttggatgtgc aggcgcgttt tgggggctgt ttgaccgata ttgtccgtca
     1021 gcgcgacgag ttttgccgaa tcgatggggt ggataatgcc caaactggtc tcttccgaat
     1081 aatcgcctgc cagcgttttg gcaaaatgct ccagcttgtg tttgtattgc agcagggatt
     1141 ccgcttcggg caacagtatt tcgcccgccc gcgtcaatac catgcctttc cccgtgcgcc
     1201 tgaacagcgg cgtgccgaca tattcttcaa gggctttaat ttgggcagaa acggcaggct
     1261 gggaaaggaa aagtcgtttg gcggcttggg taaggttgcc ctcgtgcgcg acggcgacaa
     1321 atgattttaa ttgtacggca tccatatatc cctccttgtg cggatgtttt ctatatttgt
     1381 gcaatcgaaa tcttttaggt ggattgttgc tgaaaattaa ctttttaatc aagtggtttg
     1441 taaattgtat cagttttccg gatgatggtt atcaaaaaaa aagattggtt ttattgcctg
     1501 tttgggcttt aaatggggtt acggcttccg aacgcagccc gtatcaaaaa gaaaagtcat
     1561 gcgccccttt tacgaggcgc gatatataag gaggaaggtt atggaaaaac ataatgggac
     1621 ttatcgggat ttgcaccgtc cggcttcgga atttgcgacg cgggacgaat atttggaaca
     1681 tgaattgcag attatgcaac caaaacgctg gcggcccaac ctgccctttc gcgattaccg
     1741 cttcgagtgg gaggatttga ttcctgcgat ggcgggaacg attggaaaag tggtgatggt
     1801 gggggcggtg gcggcggcgt ttgccgcacc tttggggctg cctgacagct ttgtactgga
     1861 aaatgtgcgc tatgagcttt taatcgccgc cgcgtttatc ttattggtat cgggcttttt
     1921 tctgcccggc gccaacctgc ccggtacgca cgggccgctg attccgatga ttcccatcgt
     1981 tgtgtcggca ggcgggcatc ctttggcgtt cggcatttcg attgcggttt taggtctgct
     2041 gatggcttta tttcgcggcg gcagtattat ggcgaagctg acaagcaacg gcgtatgcgg
     2101 cggattatta ctctatttgg gctttatcgg cacgacgggg caggtaaaaa aattgttttc
     2161 gtgggcaggc ggttttaata tgccctacat cgcttttacc gtcattattg taacgattgt
     2221 gatgtacgct ttgttggagc attggaaaaa acgctggtta gccgtgcctt tgggatgctt
     2281 gattgccggt gtggtggcat ttgcattggg tgcgccgttt gagtttcaca ccgcccccgg
     2341 cctgcctcca atgagtcctg cttattggtg gggtgaaaac agcggctggc atctggggtt
     2401 gccgacggca gaaagttttt tggttgtctt tccatttgcg gtattggctg ttgcaatgtg
     2461 gtcgcccgat tttttaggac atcaagtgtt ccaaaaaatc agctatccgg aaaaaaccga
     2521 taaggtattg atgaatatag acgacaccat gacaagttgt tctgtccgtc aagcagtggg
     2581 ttctatttta gggggtgcaa attttacctc ttcttgggga acttatatcg taccggcatc
     2641 gattgccaaa cgccccattc cgggcggtgc ggttttaacg gcggttttat gtattatcgc
     2701 cgggttgtgg ggctatccga tggacttggc gatttggcag ccggtattga gcgtagcctt
     2761 ggtcgtaggc gtatacttac cgcttttgga agcgggcatg gaaatgacgc gcaaaggcaa
     2821 aaccacccaa tccgccgcca tcgtggtgtt ctcttccgcc ttggtcaatc cggttttcgg
     2881 ctgggcgttg acgatgctgt tggataattt gggcttaatc ggctgcaaag agcgcagtgc
     2941 gcaattaggt ttcgccggac gcgtgttgat acccgcagta ggtttcttga tcttgtgtgt
     3001 ggcgatgggt gcggtcggga tgctgcccgg tatcccgccg tttttggaac acttcaaatc
     3061 tttgggctag gctgaaatcg gaaatgccgt ctgaaccgct ttcagacggc atttttgcaa
     3121 acaggcaaaa tgacggcggc gggatttttt attttcccga ttgaagtata atgttgccgg
     3181 gcttcaaccg gatattcaaa acggtttgtt ccaacactcg gaacggcgca taaaacgccg
     3241 cccttcgcgt tatcccgaac gggacggcta atcagattct atcgccataa aaggcggggt
     3301 ttcaaccgaa aaggaattga gatgaataaa accttgtcta ttttgccggt ggcaatctta
     3361 ctcggcggct gcgccgccgg aggcggtaac acattcggca gcttagacgg tggcacaggc
     3421 atgggcggca gcatcgtcaa aatggcggtt gggagccaat gccgtgcgga attggacaaa
     3481 cgcagcgaat ggcgtttgac cgcgctggcg atgagtgccg aaaaacaggc ggagtgggaa
     3541 aacaagattt gcgcttgcgt cgcccaagaa gcacccgaac ggatgaccgg caacgatgtg
     3601 atgcagatgc tggctccgtc cacgcgcaat caggcacttg ccgccctgac cgccaaaacg
     3661 gtttccgcct gcttcaaaca cctgtaccgc taaccttctt cagacggcat gaggcagatg
     3721 ccgtctgaag ccgttttcct gcaatttcga cagacgagaa aaatcatgaa atacatcagc
     3781 acgcgcggcg aaaccgcaca caaaccgttc agcgaagttt tattgatggg gcttgccccc
     3841 gacggcggcc tgatgctgcc ggaacattat ccgcaaatcg ggcgggaaac cttggacaaa
     3901 tggcgcggtt tggcttatcc cgaattggcg tttgaaatta tgcgcctgtt cgttaccgac
     3961 attccggagg acgatttgcg cgatattctg aaccgtactt acacggaagc ggcgttcggt
     4021 actaaggaaa tcacccccgt ccgcacgctt tcagacggca tcaaaatcca agccttgtcc
     4081 aacggccaga cgctggcgtt caaagatatg gcgatgcagt ttttgggcaa tgcgtttgaa
     4141 tatgttttaa acaaagaagg taaaaaactc aatatcttgg gcgcaaccag cggcgatacg
     4201 ggttcggctg cggaatatgc cttgcgcggc aaaaaaggcg tgaacgtatt tatgttgtcg
     4261 cccgacggta aaatgagcgc gttccaacgc gcgcagatgt acagcttgca agacgagaat
     4321 atccacaata tcgccgtgaa ggggatgttt gacgactgtc aggacattgt gaaggcggtg
     4381 cagaatgatg ccgcgttcaa ggaaaaatac catatcggta cggtcaattc gatcaactgg
     4441 ggacgcatcg tcgcgcaagt ggtttattac tttgcaggct atttcaaagc gacgcaaagc
     4501 aatgacgagc aagtcagctt ctgcgtgccg agcggcaact tcggcaacgt ttgcgcggga
     4561 cacatcgcca aacagatggg tttgcctatc cgccgcctga ttgtcgcgac caatgaaaac
     4621 gatgtgttgg acgagttttt caaaaccggc gcgtaccgtc cgcgcaacag cgcgcatact
     4681 tatgttacct ccagcccgtc gatggacatt tccaaagcgt ccaacttcga gcgtttcgtg
     4741 ttcgacctga tggatcgcga tcctcaggaa atcaatacgc tgtgggcgga agtggctgca
     4801 ggcaaaggct ttgacctgcg atttgccttg gacaaagtcg gtggcaaata cggctttact
     4861 tccggcaaat ccacccacgc cgaccgcctc gccaccatca aacaggttta cgagcaagac
     4921 caagaactca tcgacccgca cactgccgac ggcgtaaaag tcgcccgcga agtgcgtgaa
     4981 gaaggggaaa cggtggtttg tttggaaacc gcattggcgg cgaaattcga tacgaccata
     5041 cgcgaagccg tcggcgatgt cgccattccg cgccccgccg cactggaagg tttggaaaac
     5101 ctgccgcagc gcgtgcaaac cgtgccgaac agtgcggatg cggtaaaagg catcatcgaa
     5161 caaacccttg cctgatattc cgtgcagaag cattccgccc gaacttaaga ttgaaagaaa
     5221 atgaccttac ctatgcagga aacctgtttt tccatactgt tggacgaatt ggcggcaaaa
     5281 caggagaccg ccatcgcctc ccacctgctt gccgacggga gcaaggtatg gatacgcaaa
     5341 gcaggcaggc acaatgcacg atggcgttac gcgctgctcg gtatggttgc ccgatatttg
     5401 aaactggggg tgttgaagcc ggtccccagc ctcggcggcg agcctgccat tgcaaccgaa
     5461 tcaaaacgcc tgtacgaatt gcgcgcggcg gggatagccg ttcccgaatt gctcgccgtc
     5521 aggaaaaatg cccttatgtt cggcaatttg gcaggcatcc cgctcgatgc gcaaatccgc
     5581 caagaagccg aagccggaaa ggcggacgct tggcttgccg gtttggaagc cattgcacgc
     5641 gtgcataaaa aacagcagtt cctcagtcag gcgtttgcgc ggaatatgat gtcggacgag
     5701 gaaaacatca gctttttgga ttttgaagac gacccttccg aagtcctgac catagcgcaa
     5761 tgccaagccc gcgattggct ctgctacatc cattcgaccg cgctgatatt gaaaaacggc
     5821 ggactgctgg aagcggctgc ggaaaaatgg ggcggcgtac tgtctgagca gcctgccgaa
     5881 atacaaaggc tgattgccgc caccgtcaaa ccgatcctcc cgatacgcag gctggaacac
     5941 ccccgctggg gacgggacgc gctcaggctg gcagcctcga tttccctgat ttcccttgcc
     6001 gatataccgc cataagccgc cgcccgcccc gcgatgccgt ctgaagccct tcagacggca
     6061 tataaccaac gcgcatcaag cattgtcgca accatccgtt tcgtttagaa ttttggcttt
     6121 accccgaatc gggcagacat tttcactctg ccgcacatcc cgaacaagca ctatccattt
     6181 cttttactga caggaaaccc aaatggcagc attcaatacc caaaaagtat tgtccgtaca
     6241 ccactggaca gacgcatatt ttacctttac ctgcacccgc gacgaatcgt tgcgctttga
     6301 aaacggacag ttcgttatgg tcggattgat ggtggacggc aagccgctga tgcgcgcata
     6361 cagcgtcgcc tccgccaact gggaagaaca cctcgaattt ttcagcatta aagtccaaga
     6421 cggtccgctg accagccgcc tgcaacacct caaagtcggc gacgacgtgt taatcagcaa
     6481 aaaaccgacc ggaactctgg ttgccggcga cctgaatccg ggcaaacacc tttacttgtt
     6541 gagcaccggt accggcatcg cccctttctt gagcatcacc aaagaccccg agatttacga
     6601 gcaatttgaa aaaatcatcc tcgtacacgg cgtgcgctac aaaaaagatt tggcgtacta
     6661 cgaccgcttt accaaagaat tgcccgaaca cgaatacctc ggcgacttgg ttaaagaaaa
     6721 actgatttac tacccaattg tttcccgaga agaattcgaa caccacggcc gcctaaccga
     6781 cctgatggtg agcggcaaac tgtttgaaga catcggcctg cccaaaatca acccgcaaga
     6841 cgaccgcgcg atgctgtgcg gcagccccgc gatgctgaaa gacacctgca aagttttgga
     6901 cgatttcgga ctaaccgttt ctccgaaaac aggcgtgcgc ggcgattacc tgattgagcg
     6961 cgcgtttgtg gatcaataag ctgcttgaaa atgccgtctg aacggcatca gcgataaaaa
     7021 aacccgaatc ctgccaatca ggattcgggg tttttatagt gaattaaatt taaattagga
     7081 caaggcggcg agccgcagac agtacaaata gtacggcaag gcgagccaac actgtaccgg
     7141 tttaaattta attcactata tagtgggtaa aaattagaaa tgcacatttc cgtcattcac
     7201 gcgcaagtgg gaatccagac ctatccgtta attagcattt gaaaataaaa gaaaaaccaa
     7261 gcatatgggt tcccacttgc gggggaatct tcatcgtctc atttttgttt tcatcaactt
     7321 ttcctaaact ttcctgtttg gctgcataat cagacttgac tttgttattt tccatattga
     7381 gcttctctta atttgaaaaa attaacataa acatttaaaa ataaaacaaa attatataat
     7441 aaaaaaaata atgagaatat aaaggataaa aagagagtta agttatttaa tcggacttgt
     7501 tctctataaa tcataagaca ccaaataagc cataataaat ttagtaaata aaaactgctt
     7561 atacccaact ttcttttctt ggaacctatt attatttcaa ctccggatga aaaaatagaa
     7621 tttaaaaatt tttttctttg atatatcgtt aatatctccc tagtttttct taaaaaaaat
     7681 ataatcatat atatactaca caacattggt aataaataaa ttaaaagcga aaggtaatta
     7741 aattgataat tgtaaattat ataataaaca acatacgaag aaaaaattat tatagatgat
     7801 ttactatacg aataaaaaaa tatcctacct agcccgaacc ttcttataat ttgtactatg
     7861 tgattatcca tttcaatatc catttaagct ttcaattaat tttctatatt tttcaatggc
     7921 atgtatcatg ctatgcttgg gaaatatcat cttggttgat gatcaagcca tgtatgcaaa
     7981 gattggaata tggtatttgt cgtgctaata atgcccccgt tactgttata cgtctaaagc
     8041 aacgctgtcc gtcattgttc ggacggcatt ttttcagacg gcatgtccac ctgcttgtga
     8101 agccgcatag cattgcccaa tacgaccagc gaactgaagc tcatacccag tgcggcaatc
     8161 cacggttgga catagcccaa aacggcaagc ggtacggcaa tgatattgta cgcgcccgcc
     8221 catatcaggt tttgccggat aatatggcgg gtgcgccgcg cctgatcgag caggtgggcg
     8281 acggtacgca aatcttcgtt caataacaca atgtccgcgc cgtccctcgc aatatccgtc
     8341 ccgcccgctg cggcggcgga tacgtctgcc tgcgccaaaa cgggcgcgtc gttgatgccg
     8401 tcgcctatca tcagcacttt tttcccttct ttttgcaagg ctttgacgta ttccagtttg
     8461 tcctcgggca tggcttgggc gcggtagtgc gcgacaccca gggcgcgtgc ggtttcggca
     8521 acggcggttt cgcggtcgcc gctgaggatg tgcagggtca ggtttttgcc tgccaactgc
     8581 cgcaccgcct ccgccgcgct gtctttcaag gggtcggtca ggtagaacac ggcttggaaa
     8641 ccgctttgac tgccgaggta aaccgcgctg ccgccgcctt ctgtttgcgg ttctttacct
     8701 gaaatttcgg cgacatagga tgccctgccc aatgcccaaa cctgtgtttc gccgttgacg
     8761 gtcagttgcg cgcccacgcc ttcgccgatg cggttgaggc gttgtttaat agcgatgtcg
     8821 gggacgctgc cgcttgaaac acggcagttg aggatggcgc gggcaagggg atgttcggac
     8881 tgttgttcta aagcctgcgc caccgcgaga acaaaggctt cgtctgtgcc tctcaacaat
     8941 gagatacggc ggacggcggg tttgccttgg gtcagcgtgc cggttttgtc gaagatgatg
     9001 tcggtggttt gggcgagggt ttcgattgcc tgctttccgc cgattaaaat gccttcgcgc
     9061 gccagcgtac cggtagaagc tgccagcgcg gtcggcgtgg caagcgataa ggcgcagggg
     9121 caggtaatga ccagcagggc gacggtaatc cacaatgcgg tgtgcgcgtc ggcgtacagc
     9181 gtccagccga tgaagacggg gacggcaagc aggagttcgc cgaatatgaa agacgaggcg
     9241 tattgttccg ccaactcggc agtgcgcggt ttttgcgcta aggcgcggtc gagcaggcgg
     9301 acgatgtgcg acagtcgcgt gccgccgccg gtgcggtcgg tgcgtataat cagggggctt
     9361 tgcgtgttga gtgtgccggc ggttactttt tcagacggca ttttggcgac gggcaggctc
     9421 tcgccggtca gcatagattc gttgacggca ctgcttcctt ccagcaccgt gccgtcaacg
     9481 gggatggttt cgcccggttt gaccagcacc acatcgcctg cttggagctt gacgacggcg
     9541 gcttcgcgga catcctgtac ggcaggatag ccgggcatcc ggtggcagaa ggcgggtatc
     9601 agcttcacca gcctttcggc ggcatcgcct gccttgcgtc gggcaatgtg ttccataaag
     9661 cgtccgccca gcaggaaaaa cagcagcatg gctatggatt cgaaatacat cccctgcccc
     9721 gcattggtcg caaggctgta aacgccggcg ataaaggtca tgatgatggc gacggcaatc
     9781 ggcgtatcca tgccggcgcg gcggtttttc aggtcgcgca acgcgccttg ataaaacggg
     9841 acggcgcaat agaatacgac gggcagcacc attaaaaagc cgccccaatg gaggatttgc
     9901 aggaaatcgg gttcgatgtc gccgccgtaa aggtaggtcg gcagcgcgaa catcatcgtc
     9961 tgcatcatcc ccagcccggc aacggcgagg cggacgatgt attgtttgcg ttctttttgg
    10021 ttggcggctt cgattttttg cgcgtcatag ggtgcggcgg tgtagcctat ctgccggatt
    10081 ttcaacagaa tatcggaaag gcggattttg ccgtcgtccc agacgacgcg gcagcggtgc
    10141 gtgctgtaat tgaggtcgat gcggacgatg ccgtctgtac gcaaaagctg ctgttcgatc
    10201 agccagacgc aggcggcgca ggtgatgccg ccgagcatta aaaccgcctc gcgcgtgccg
    10261 ccgtgggttt ccacaaagtc ggactggact tcgggcaggt cgtacaggcg gatttggtcg
    10321 aggatttctt ggggcggcag ctcggttttt tgcgcgtcgg cggtgcgttg tttgtaataa
    10381 ctgcccaagc ccgcgtcaat aatgctttgt gcgaccgcct gacagccggc gcagcaggtt
    10441 tctcggtctt cgccttcgta gcggacggtc agatgcaggt tttcgggaac atccagcccg
    10501 cagtggaaac aggttttttt catggcattt cggtttcgtc tgtgtttggt gcggcggcac
    10561 aagactcggc aatggcttcg cgcagtgcgt ctataccggt attttcagca acggaaatgc
    10621 ggacggcggc aatttttccc gcagcgtcgc gccatatgcc cgtgttttgt tcttcagacg
    10681 gcagcaggtc ggttttgttg tacaccttga tgcacggaat atcgccggca tggatttctt
    10741 gcagtacgtt ttccacgtct tcaatctgct gtccgctgtt cggggcggcg gcatcgacga
    10801 cgtgcagcag cacatcggct tgcgcggttt cttccagcgt ggcggaaaat gccgaaatca
    10861 gtttgtgcgg caaatcactg acgaatccga cggtatcggt caggataatg ctgcattcgg
    10921 gactgatgta cagccgccgc gccgtggtgt cgagtgtggc gaaaagctgg tctttcgcat
    10981 atatgcccga cttggtcagc cggttgaaca ggctggattt gccgacattg gtatagccga
    11041 ccagcgcaaa cgttttgatt gtgcccgatt cgcggggctt gcggcgcagg gcgcgctgtt
    11101 ttttgaggtt gacaagctgt tttttcaagg cattgatccg atgggcgatc aatcggcggt
    11161 cggtttccag tttggtttcg ccggggcctt tcatgccgat accgccgcgc tggctctgca
    11221 gatggccgta accgcgtatc aagcgtcccg ccaaatggct caattgcgcc aactcgactt
    11281 gcagcctgcc ttcctgcgtg cgggcgcggc gggcgaaaat cgccagaatc agccctaccc
    11341 tgtccaatac gcggcatttc agttcttttt caaggttgcg ttcctgcgtg ggcgtgagtt
    11401 cgtggttgaa tacgaccaaa tcgatgccgt ctgcggcaac tgcttctgac agctccgccg
    11461 ccttgcccgt gccgacaaac agcgcggtgt gcggacggtc gcgtttggca gtctccacgc
    11521 gcacggaatc gccgcccgcc gctttgacca gctcgacagc ttccgccaat gccgtctgaa
    11581 aaccgttcag acgggtggca ctgctgcccg tgccatcttt gtccaacatt acgcccacca
    11641 gcatgacgcg ttcgggttgc gcttgggtac tgccgttgcg gcctgttctg cctgtcatgt
    11701 attcaatcct gtaaataaag ggttacgcct cgttccgttt ctccttccgg tgcaagcgtg
    11761 atgcggctgc ccgccattcc gtcaacgctg aagacgatgc cgcccgcctc gtgccttcct
    11821 atggacaggc ggctgccggg tcgctgccgg taggtataac gggtacacgc ttccaaacac
    11881 cagcgtccgc gccagtggat gaaatatttt tcgccttttc taaccgcaag tgtctcgccg
    11941 ccgtttacct caaaataagg gtaatcgggt tttcgggttc taaactgcca tttcgcctgc
    12001 gcgcgccgtc cgttgcggac atagtcgaat accgccgtat aaagcgtacc gtattccaaa
    12061 ggtttgagcg gaaaaagcgc gaattggtac gcggtcagcc tgccgttggg gtcgttgccg
    12121 gcggttaaaa ccctgacggg gcggatttcg tttttaccct gatacagctt gaaacttttc
    12181 atcgtaattt tgcctgccgc ctcggaaaaa tcaatgctgg caggattgcc cgtgatttca
    12241 tattccggca cggggtctgg acgctcgccg tggaaataag gcagtgcgcc gctgccgacg
    12301 ggataggctg tatagagcag ctcctgtgcg ggcatggctt cgtcggtgta cacgaccgca
    12361 ccgttatggc aggcgttgcg gtaatatttc cgtcctgctt ccggctgatt tctgccttgg
    12421 gcgcaatgcc gctcaaacct gccgttgccc tgattgaata cgagaacggt tttaccgttt
    12481 tcgcgcacaa atgccgctcc tgcctcatcc gtatggcggt caagtaggga aaggcggtgg
    12541 tagattgcgc tcattaatcc gtccacttgg cgttgctgcg tgcggatgtc gctgtcggac
    12601 gattcggcgg cttcctcttc cgtgctgatg ttttcatgca cgccgttgta gagatacccg
    12661 gcaaggcgtg tgcgttcggt cagcttttgt gcggtgtagt gcggattgtc gggatggtgt
    12721 tcgccgtgtc cgtcttcggg attgagcgtg agatagcgtg cgtgcctgcg ggcggaattt
    12781 tccaaaaccg gcgcgtgtgc cagcttgtgc aaaccgattt gggcgcggat gcggttgagg
    12841 tagtgcagcg catcgccgcc gtccctgatt tgcggtgcgg acggatagac aagttcgccc
    12901 gcgggcaggg attggttttg ggtatgatag aaaacgcctg ccgccgaacc caacaggagc
    12961 agccgaataa aaagggattt cataatgccg tcctcccgac gcggcaacgg ggtgttttat
    13021 caaaacggca aacttgccaa tgcggtttcc gcttgccgat tcccaaaccg gcaaaccttt
    13081 ccccgtgccg atgccgaacc cgatgccgtc tgaaccttca gacggcatcg ggtgtttatt
    13141 tgtccacccg gatgggtgca ggttcgtatt gtgtcgattc gtcgccgtaa tacagcacgc
    13201 cgagtttgat ggggatgcgt ccctgcgatt tgcggtgggc attggaatcc ctcagggaat
    13261 aggcgcaacc gcaatattcc tgctggtaga agttttcgcg tttgctgatt tcaatcatgc
    13321 gcgcgctgcc gccgcctttg cgccagttga aatcccaata caccacatca tcgtaaggcg
    13381 cggcggcgcg gtgtccgcag tcgttgattt gcgccatatt tttccagcgt gaaatgccca
    13441 gcgaactggt aaagacgggg aacccgtgtt catgcgcgta ttgcgcggct ttttcaaaac
    13501 gcatatcgaa acacatggtg cagcggatgc cgcgttccgg ctcaaactcc atgcctttgg
    13561 ctttggcaaa ccattctttg cggtcgtttt cgtagtcgtc gtctttatcg atgaaaggaa
    13621 tgccgaactt ttccgcaaag cgcacgtttt cctcttttcg gagcatatac tctttatgcg
    13681 gatggatatt gggattgtaa aaataaatgg tgtaatcaat gccgctggcc agcatggctt
    13741 ccatcacttc gccgctgcac ggggcgcagc aggaatgcag caagactttt ttatgtccgc
    13801 cgggcgggac gagtatgggg cggtcaatgt cggtaacggt aggtttgttt tgtgtttcca
    13861 ttaacttaac aatatctgtc gaatacggtt gaagcggcta ttttatcaga gccgccgcat
    13921 gtaaaacagg gtgtcagggc atcagcccga gctgcctgaa cgaacggacg gtttgcgccg
    13981 ttacgataaa atggtcgagc agcgacacat cgaccagcga catggcctgt gccagccgcc
    14041 ttgtgaacat gatgtcttcc tgcgaaggtt cgggcgagcc gcccggatgg ttgtgcgcaa
    14101 taatcaggct gtcggcatat tcgtccaatg ccagtttgac gatttcgcgg atgtaaatcg
    14161 tgttttccgc aaccgtaccg cgcgacagct ctctgaccgc aatcagttgg ttttggcggt
    14221 tcagcagcag cgcgacgctg acttcgactt tttcctgccc caaatgaaag cgtaaataat
    14281 cggcaacggt atccggatcg gacaggacga tgctctcctg caattcttcc gccaatatcc
    14341 gccgcccgat ttccctgact acggcaaact gtgtgaaact tgccgtcccc atccctttgt
    14401 atgccgacag tttgccgacc tccgcgctca tcagcctccc caaactgccg aactcctgca
    14461 gcaaataacg cgccaaatcg accgcactca ttccgcgcgt gccgacgcgc agcaggattg
    14521 ccaaaagttc ggcatcactc aaagccgccg ccccgcgttc caacagcttt tccctgggtc
    14581 tttcgccttc cggccattgc ttgatgctca tgttttccct tttttaaata gtctgagatg
    14641 ccgtctgaaa caaatgttcc cacacgccaa atatccgaaa tgccgtctga accggatttc
    14701 cgcttcagac ggcatctgta tttcaaaaca gtcttacgcc gtttcttcca actcgatgga
    14761 cgccgccaga agcgacaggc gtgccattac gccgtaaacg tagaggcggt tgtgttcgtt
    14821 gtccacgtcg cagtcttttt cagagcgcgg catacccagc gagtcccatt gttcgaatac
    14881 gcgcttgcag gcttcgggag cttcttggga gttgtcgccg ttgccggtgg ggatgctgtt
    14941 ggacagcgga acaaacacca taccgccggc gttgaggttt tcgtccgcac cgcgcccttc
    15001 gtgtacgcgg aaaaagccgc cgatgacgaa gcggtccatc atatagacga cgggttcgca
    15061 caccgcgccg tttaaggttt cataagtata aatcccttct tggacaatca cttcgctgac
    15121 ttccaagcct tctttgactt tcgccatttt attgcggttt ttacggttca atccgcgcac
    15181 ttcgtcggcg gatttgacgc tcatcacgcc catgccgtaa gtgccggcat cggctttgac
    15241 gatgacgaaa ggtttgtcgg taatgcccga ttcgtcgtat ttggcttgaa ttttcgccag
    15301 cacacgttct accgcttccg ccaacgcgtc ttcgccttca cgcccttgga agtccaaacc
    15361 gccgattttt tcaaaataag ggttaatttg ccattcgtcg atgccgatta acttggaaaa
    15421 ttcggcggta acttggttgt acgcgccgaa atgatttgtt ttgcggcgcg tcgtccaacc
    15481 gccgtgcaac ggcggcaaaa cggtttggct gatgcctttg aggatgtcgg gaatgccggc
    15541 agacaaatcg ttgttcaaca aaaccacgca aggcgaaaag ccgtctgcaa gatggacgcg
    15601 gtcgcgggta cgcaataaag gttccaacag gattttgtcg cccaatgcag tttcaaattc
    15661 ggtcggttcg gttacttccg gattcaggct gcccaagcgc acttcatacc ctgccgaacg
    15721 caaaatctcg ccgagggcgt aaatgttttg caggtaaaac gtattgcgcg tgtggttttc
    15781 gggaataatc aatacggatt tcgccgtttc gcaggcgcgt tgcaccgcat cttgcgcggc
    15841 aaccgccgcc agcgggataa agttgggatt cagattattg aagccgccgg ggaacaaatt
    15901 catatcgata gacgaaattt tgtaaccggc attgcgtata tcgaccgaac cgtaaaacgg
    15961 cgggcggtgt acgctccact gcgtgcggaa ccacgcctcg attttggcgt gattggacag
    16021 gatttttgcc tcaaacgcct gaagttgcgc cgaatgttcg ggcgacatca ccggtaattt
    16081 catctttcgt ctgcctctgt gtgttttttg acaagatgca gatgataatg cctttgaaag
    16141 aatgacgaca agtattcttt cgtatcgaat catgctttag atataactgt ttactgaaat
    16201 ggacaatacg tctaattttt tcgttaaaat tgtttgcaaa ataacaccgg ctcaaaaatt
    16261 agacaaaatt tgttgcgcgg tataaagaat acgtctaaaa tccgcccaat ccgcattacc
    16321 atttatccaa aagaacaaca tcatgacagc acaaaccctc tacgacaaac tttggaacag
    16381 ccacgtcgtc cgcgaagaag aagacggcac cgtcctgctc tacatcgacc gccatttggt
    16441 gcacgaagtt accagccctc aggcatttga aggcttgaaa atggcggggc gcaagctgtg
    16501 gcgcatcgac agcgtcgtct ccaccgccga ccacaacacc ccgaccggcg attgggacaa
    16561 aggcatccaa gacccgattt ccaagctgca agtcgatact ttggacaaaa acattaaaga
    16621 gtttggcgca ctcgcctatt ttccgtttat ggacaaaggt cagggcatcg tacacgttat
    16681 gggccccgaa caaggcgcga ccctgcccgg tatgaccgtc gtctgcggcg actcgcacac
    16741 ttccacccac ggcgcattcg gcgcactggc gcacggcatc ggcacttccg aagtcgagca
    16801 cacgatggcg acccagtgca ttaccgcgaa aaaatccaaa tccatgctga ttgccgttga
    16861 cggcaaatta aaagcgggcg ttaccgccaa agacgtggca ctttacatca tcgggcaaat
    16921 cggcacggcg ggcggtacag gctacgccgt cgagtttggc ggcgaagcca tccgcagcct
    16981 ttctatggaa ggccgcatga ctttatgcaa tatggcgatc gaagccggtg cgcgctcagg
    17041 catggttgcc gtcgaccaaa ccaccatcga ctatgtaaaa gataaaccct tcgcacccga
    17101 aggcgaagcg tgggacaaag ccgtcgagta ctggcgcacg ctggtgtctg acgaaggtgc
    17161 ggtattcgac aaagaatacc gtttcaacgc cgaagacatc gaaccgcaag tcacttgggg
    17221 tacctcgcct gaaatggttt tagacatcag cagcaaagtg ccgaatcctg ccgaagaaac
    17281 cgatccggtc aaacgcagcg gtatggaacg cgcccttgaa tacatgggct tgaaagccgg
    17341 tacgccatta aacgaaatcc ccgtcgacat cgtattcatc ggctcttgca ccaacagccg
    17401 catcgaagac ttgcgcgaag ccgccgccat cgccaaagac cgcaaaaaag ccgccaacgt
    17461 acagcgcgtg ttaatcgtcc ccggctccgg tttggttaaa gaacaagccg aaaaagaagg
    17521 cttggacaaa attttcattg aagccggttt tgaatggcgc gaaccgggct gttcaatgtg
    17581 tctcgccatg aacgccgacc gcctcgcacc ggggcaacgc tgcgcctcca cctccaaccg
    17641 caattttgaa ggccgtcaag gcaacggcgg acgtacccac ctcgtcagcc ccgctatggc
    17701 agccgccgcc gccgttaccg gccgctttac cgacatccgc atgatggcgt aagtattttc
    17761 aggcggtctt taagaatttg acgcggccgt ctgaaatata tcccgtaagg cagaaacacc
    17821 tttaatattc aaaccaagga gtttaaccat gaacaaactt ttcattaccg ccctgtccgc
    17881 cctcgccttg tccgcctgcg ccggcacttg ggagggcgcg aaacaagaca ccgcccgcaa
    17941 ccttgacaaa acacaggccg ccgccgaacg cgccgccgaa caaacaggca acgccgtcga
    18001 aaaaggctgg gacaaaacca aagaagccgt caaaaaaggc ggcaatgccg tcggacgcgg
    18061 catttcccat ctcggcggaa aaatcgaaaa cgccaccgaa taaccgattc gcgatgccgt
    18121 ctgaaaccgc ttcagacggc atcgtcagca caagacgcaa acccatgaaa gcctttacca
    18181 aaattaccgc catcgtcgcc ccgctcgacc gcagcaacgt cgataccgac gccatcatcc
    18241 ccaaacaatt tttaaaatcc atcaaacgca gcggcttcgg ccccaatgcc tttgacgaat
    18301 ggcgttacct cgaccacggc gaaccaggca tggacaacag caaacgcccg ttgaaccctg
    18361 atttctcgct gaaccagccg cgttaccaag gcgcgcaaat cctgttgacg cgtaaaaact
    18421 tcggttgcgg ctcttcacgc gaacacgccc cttgggcatt ggacgactac ggcttccgcg
    18481 ccgtcatcgc ccccagcttc gccgacatct tctttaacaa ctgctacaaa aacggccttt
    18541 tacctatcgt gttgaccgaa gaacgggttg accgactttt caaagaagtt gaagccaacg
    18601 aaggctatca gctctccatc gaccttgccg aacaaaccct gaccacacca agcggcgaaa
    18661 cgttcacatt tgacattacc gaacaccgca aacactgcct cttaaacggc ttggacgaaa
    18721 tcggtctgac cctgcaacac gccgacgaaa ttcacgcctt tgaagaaaaa cgccgccaaa
    18781 gccagccttg gctgtttaac ggttaaaacc tataccgtct gaaggccgtt cagacggcat
    18841 tcctgataac atataattgt tgcggctaat gtaaacagag gggcgataaa ttgctttcat
    18901 cttttaagcg ggcttagcga cactctaaat taatgcaata atgcaactct ccatttcaaa
    18961 taaattttta gatttttaat atgcaacaaa ttaaatttat tgacttattt tccgaaatga
    19021 gtggcatcag gaaaggattc gaacaagcct gtcggaaaca gtcggttgct tgcgaatgtg
    19081 tttttacttc agaaatcaaa ccggcagctt tggaagtatt gaagcaaaac taccctgatg
    19141 aagtgccgta tggagatata acgaaaattg aaacagggga tattcccgat tttgacatcc
    19201 tgttggcagg cttcccttgt caggcttttt ctttcgcctg aaaaagatta ggctttgaag
    19261 atacacgggg aacgcttttc tttgatgtgg caaggatttt aaaggcaaaa aaaccaaaag
    19321 gttttatttt ggaaaatgtg gaaggattgg tgacacacga tagaaaagat tcgacacaaa
    19381 aaatagggcg caccctgacc gttattttgg aaaccttgga agcattgggc tactatgttt
    19441 cttggaaagt tttaaatgca aaagatttcg gcattcccca aaacaggaag cgcatttatc
    19501 tgacaggcag tttgaaatcc aaacccgact tatctttcga aacaagccca agtccgaaat
    19561 taaaaaatat tttggaatcg ggactgccta ccgaaagcag ccctttcatc aaaaaattgc
    19621 taaaaaaatt tcccccgtcc gaactgtacg gaaaatcagt aaaagacaaa cggggaggga
    19681 aaaacaatat tcacagttgg gatattgaat taaaaggcgc agtaaccgaa gaagagaagc
    19741 aattgttaaa tatccttcta aaagaacgga ggaaaaaaaa tgggcttcag aaatcggcat
    19801 agattggatg gatgggatgc ctttgacaaa agcgcaaatt tcaactttct ataaacaccc
    19861 cgatctccaa aatattttgg acagcctgac agataaaggc tatttggttt tagagcatcc
    19921 gaaacaaaaa attggcgggc aaagaatcaa agatgaatcc ctgcccaaag gctacaatat
    19981 tgtttcaggc aaaaaatctt ttgaaatcaa taaaatatta gatcaaaacg atgttgcgcc
    20041 aaccttggtt gcaatgaata tggagcacct tttcgtcgtt gacaacggcg gtttgcgtac
    20101 acttaccgga aaagaagggt tgcgcttatt cggctatccg gacgattatt cgtttgatat
    20161 tcccaaaaaa gacagatgcg atttattggg taataccgtt gccgtccctg tgattaaggc
    20221 ggtatctgaa agacttctgc atactttata aaccgacagg gataaaatga taaaacttac
    20281 tgcacaacaa atatttgata agttgctgga tgaagaaaaa atcttatcgg ccaatggtca
    20341 aattagattt ttcttaggcg atgtggatat tatcgtcaaa caaaaagatg ttgtcggcaa
    20401 catcattcag gaatggctcg gcggatgatt gagaaaaagg gaaattgaat ttgatgtttc
    20461 aaccaatacc caaatgcctc ccgatttctt tttaaataaa aaagaccgca gcagagaatt
    20521 gttggaagta aaggcgttca accgaaatgc cggcccgggt tttgatattg cagattttaa
    20581 aatgtattct gataaaatca ttcataagcc ctatatgctg gatgtagact atttaatatt
    20641 cggttacgat atggacgaca acggcaatgt aaccatcaag gatttatggc ttaaaaaagt
    20701 atggcaaatt acgagaagca tggatgggtg ggcaattaat cttcaagtca aaaaaggcgt
    20761 ggtgcataaa atccgcctgg gtgtttggta cagcataaat aaaaagaata tgcctatgtt
    20821 tgaatgcttg gaagattttg tttccgcaat tgaagaaacc gtttatcaaa acccggcaac
    20881 acggcataac gcatccttat ggaaaaagaa atttgaggaa gcctataaaa aacactacaa
    20941 ccgatcgatt tccatacctc gttggcacga aattgcacac aaatacaaaa agaaataacc
    21001 cttcaaccat caacccataa ggaaacttga aatgaccaaa catatcgcca tcctccgagg
    21061 tgacggcatc ggtcccgaaa tcgtcgccga aaccgtccgc gtactcgaca aactcatcgc
    21121 ccaaggtttg gatgccggct acgaatacgc cccattgggc ggcgaagcct atgacgaata
    21181 cggtcatcct tatcccgaat ttacgcaaaa cctctgccgc aaagccgatg ccgtcctgct
    21241 tggtgcagtc ggttctcctc aatacgacaa cctcgaccgt ccgttgcgcc ctgagcgcgg
    21301 cctgttggca atccgtaagg atttgaacct gtttgccaat ttgcgtcctg ctgttttata
    21361 tcccgaatta gccaacgcct ccacgctgaa gcctgaaatc gttgctggtt tgaatatttt
    21421 gattgtgcgc gaactcaccg gcgacatcta tttcggcgaa ccgcgcggca tccgcgttct
    21481 ggaaaacggc gaacgcgaag gctacaacac catgaaatac agcgaaagcg aaatccgccg
    21541 catcgcccac gtcgctttcc aatccgccca aaaacgcagc aaaaaagtct gctccgtagg
    21601 caaagccaac gttttggaaa ccaccgaact gtggcgcgaa atctttgaag aaatcggcaa
    21661 acaatatccc gatgtcgaac tttcccatat gtatgtggac aacgccgcta tgcagctcgt
    21721 gcgcgcgccc aaacagtttg acgtgattgc caccggcaac atcttcggcg acatcctctc
    21781 cgacgaagcc tccatgctga ccggctccat cggtatgctg ccttccgctt ctttggacga
    21841 aaacggcaaa ggcctgtacg aaccgtccca cggctccgct cccgacatcg ccggacaaaa
    21901 caaagccaac ccgctggcca ccatcctctc gctcgcaatg ttgctgcgtt acagcctgaa
    21961 cgacgaagcg cgcgcgcaac aagttgaaaa cgccgtccaa aaagtgctgc aacaaggctt
    22021 gcgtaccagc gacatttacg aagaaggcac aaaactcgtt tcctgctccg aaatgggcga
    22081 tgcggtactc gccgccttgt aaaaaaacaa agcgcggatc acaccgcgct ttttcatctg
    22141 cccttttccg ctcgccccgt tcgtcaaccg cctgcaaagc caccgtttac cggcaggaaa
    22201 tgccataaat ccccgcctat gttaggataa acgcctttac tggctcacac aaaggattat
    22261 catcatgaaa aaaatcatct tcgccgcact cgcagcggca gccgttggca ctgcctccgc
    22321 cgccacctac aaagtggacg aatatcacgc caacgcccgt ttcgccatcg accatttcaa
    22381 caccagcacc aacgtcggcg gtttttacgg tctgaccggt tccgtcgagt tcgaccaagc
    22441 aaaacgcgac ggtaaaatcg acatcaccat ccccgttgcc aacctgcaaa gcggttcgca
    22501 acactttacc gaccacctga aatcagccga catcttcgat gccgcccaat atccggacat
    22561 ccgctttgtt tccaccaaat tcaacttcaa cggcaaaaaa ctggtttccg ttgacggcaa
    22621 cctgaccatg cacggcaaaa ccgcccccgt caaactcaaa gccgaaaaat tcaactgcta
    22681 ccaaagcccg atggcgaaaa ccgaagtttg cggcggcgac ttcagcacca ccatcgaccg
    22741 caccaaatgg ggcgtggact acctcgttaa cgttggtatg accaaaagcg tccgcatcga
    22801 catccaaatc gaggcagcca aacaataaat cttgatgcga atcagcacaa atgccgtctt
    22861 catcaaaccg ccccgccaac ggtaagccga tatggcggag cacaatccgc cgaaagatat
    22921 ttcagcggca ggcagggctt gccggaaagt ccggaaagta tagtggatta acaaaaacca
    22981 gtacggcgtt
//
LOCUS       DAWHRA010000015        22327 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWHRA010000015 DAWHRA010000000
VERSION     DAWHRA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22327
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.015"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(201..1976)
                     /gene="ptsP"
                     /locus_tag="WIB30_000434"
     CDS             complement(201..1976)
                     /gene="ptsP"
                     /locus_tag="WIB30_000434"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ3796566.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTARRQLAERGDAFGDVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQINSVSVEEEADFKGRK"
     gene            complement(1976..2245)
                     /locus_tag="WIB30_000435"
     CDS             complement(1976..2245)
                     /locus_tag="WIB30_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ3796567.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFQSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2314..2751)
                     /locus_tag="WIB30_000436"
     CDS             complement(2314..2751)
                     /locus_tag="WIB30_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ3796568.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2832..3395)
                     /locus_tag="WIB30_000437"
     CDS             complement(2832..3395)
                     /locus_tag="WIB30_000437"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ3796569.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3464..4288)
                     /locus_tag="WIB30_000438"
     CDS             complement(3464..4288)
                     /locus_tag="WIB30_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ3796570.1"
                     /translation="MIKKTIGGIIPIFMAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISAAVRS
                     ASSDWRGIRLHVFDVPKAQGNLYRRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRKAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRVRTDR"
     gene            4380..5012
                     /locus_tag="WIB30_000439"
     CDS             4380..5012
                     /locus_tag="WIB30_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ3796571.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5218..7002)
                     /gene="rmuC"
                     /locus_tag="WIB30_000440"
     CDS             complement(5218..7002)
                     /gene="rmuC"
                     /locus_tag="WIB30_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ3796572.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRLQGEFADLNAQLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(7341..8129)
                     /locus_tag="WIB30_000441"
     CDS             complement(7341..8129)
                     /locus_tag="WIB30_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ3796573.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8144..9493)
                     /locus_tag="WIB30_000442"
     CDS             complement(8144..9493)
                     /locus_tag="WIB30_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ3796574.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9512..10093)
                     /gene="petA"
                     /locus_tag="WIB30_000443"
     CDS             complement(9512..10093)
                     /gene="petA"
                     /locus_tag="WIB30_000443"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ3796575.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10216..10965)
                     /locus_tag="WIB30_000444"
     CDS             complement(10216..10965)
                     /locus_tag="WIB30_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ3796576.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11096..12025
                     /locus_tag="WIB30_000445"
     CDS             11096..12025
                     /locus_tag="WIB30_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ3796577.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(12280..12672)
                     /gene="rpsI"
                     /locus_tag="WIB30_000446"
     CDS             complement(12280..12672)
                     /gene="rpsI"
                     /locus_tag="WIB30_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ3796578.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12682..13113)
                     /gene="rplM"
                     /locus_tag="WIB30_000447"
     CDS             complement(12682..13113)
                     /gene="rplM"
                     /locus_tag="WIB30_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ3796579.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13291..13470)
                     /gene="ssrS"
                     /locus_tag="WIB30_000448"
     ncRNA           complement(13291..13470)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WIB30_000448"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13476..13781)
                     /locus_tag="WIB30_000449"
     CDS             complement(13476..13781)
                     /locus_tag="WIB30_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ3796580.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13792..14121)
                     /locus_tag="WIB30_000450"
     CDS             complement(13792..14121)
                     /locus_tag="WIB30_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796581.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(14176..15165)
                     /locus_tag="WIB30_000451"
     CDS             complement(14176..15165)
                     /locus_tag="WIB30_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ3796582.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15288..17990)
                     /gene="ppc"
                     /locus_tag="WIB30_000452"
     CDS             complement(15288..17990)
                     /gene="ppc"
                     /locus_tag="WIB30_000452"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ3796583.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNGDVMALSDKSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAVLLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18033..18926
                     /locus_tag="WIB30_000453"
     CDS             18033..18926
                     /locus_tag="WIB30_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ3796584.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALAGRLKRINHTVNVHTVNEKLDGCRL
                     TDLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGTER"
     gene            complement(19336..19560)
                     /locus_tag="WIB30_000454"
     CDS             complement(19336..19560)
                     /locus_tag="WIB30_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ3796585.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDTQEPYSLRDEIPPHY"
     gene            complement(19561..20949)
                     /locus_tag="WIB30_000455"
     CDS             complement(19561..20949)
                     /locus_tag="WIB30_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ3796586.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLTGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(21035..21856)
                     /gene="prmC"
                     /locus_tag="WIB30_000456"
     CDS             complement(21035..21856)
                     /gene="prmC"
                     /locus_tag="WIB30_000456"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ3796587.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(21846..22307)
                     /locus_tag="WIB30_000457"
     CDS             complement(21846..22307)
                     /locus_tag="WIB30_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796588.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAAFWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVASIGTFAGKNKERKAASAADGTKNDV"
BASE COUNT         4868 a         6230 c         5866 g         5363 t
ORIGIN      
        1 tttacccgac aagtttccgt gccgacagac ctagattccc gcctgcgcgg gaatgacgga
       61 ttttaggttt ctgattttgg ttttctgttt ttgagggaat ggccgatttt gggtttctgt
      121 ttcggttttc tattttgcaa gaatggcaaa atttcagatt gcgggcattg ttaagtattt
      181 ctatttttca cctgccgtat ttatttccgc cctttgaagt cggcttcttc ctcgacagac
      241 acgctgttga tctgtttgat cagcttttcc gacttctctt cgtcttcgca gcggatgact
      301 ttcacaatat cgctttcgag ctgcccgaca ttgctgtgca gaatgatgtt tttgacgggc
      361 aggatgttgt tggggttcat ggaaaaacgg cgcagcccca tacccaataa aacgcgggta
      421 aacgcggtat cgcccgccat ctcgccgcat acggacacgt ctttgtccat gcggttggcg
      481 gtgcggatga cgtgttgcag cattttcagc acggcgggat gtccgggctg gtaaagatgg
      541 ctgacgctgt cgtcgccgcg atcgacggac aagatgtatt gaatcaggtc gttggtaccg
      601 acggagatga aatcgaccag tttcaaaata ctgccgacgg tcagcgcggc agacggaatt
      661 tcaatcatgc agccgatgcc gacatcgccg aaggcatcgc cgcgttcggc aagctggcgg
      721 cgggcggtgt ccaaatgaat caggcactgg cgcacttcgg atacggaggt aatcatcggc
      781 cacatcatcc gcacagggcc gtgtaccgcc gcacggagga tggcgcgcat ctgggtgcgg
      841 aacatgaccg gttcggcaag gcacaggcgg atgccggtca tgcccagcgc ggggttgagg
      901 ctgccgttgg gcgtgctgtt tttcccgaac cagcgggggt ttttatccac gcccaaatca
      961 acggtgcgga tggtcacgct tttgcccttc atttttttga caatcgcgct gtacacttcg
     1021 tactgctcgt cttcagacgg catcgtatcg cggttcaggt aaagaaactc gctgcggaac
     1081 agcccgatgc cgtctgcgcc gaggttgtgc agcggtttca cgtcttcggc ggattctata
     1141 ttgcccacaa gctcgatgca gaccccgtcg gcggtggcgg cggcggtttt tttgagcttg
     1201 ttcaaatcgc gtttgtggct gcggtattcg cgggcacggc ggcggtattc gttcaacacc
     1261 gactcatccg gcgcgataat caacacgccg ttgataccgt ccacaatgac cgtttcgcct
     1321 tcggtaatca gtttgcgcgc gttgtgcagc ccgacgacgg acgggatgtc caagctcctg
     1381 cccaaaatcg ccgtatgccc ggtggggccg ccggcatcgg taacgaaggc ggcaatgcgc
     1441 tgctctttaa acaaaaccgt gtcggcgggc gaaaggtcgt ttgcaatcag aacggtttcg
     1501 tcaaacaggt tgtcggcaac ttccaactcg ttgccctgcc cgatcaggtt gttgtggatg
     1561 cggcggacga cttgcagcat atcctgcttg cgttcgcgca aataggcatc gtccatattg
     1621 tcgaattggg cggcgagttt gtcgctctgc tgcttcaatg cccactcggc gttgattttt
     1681 tgttccctta aaatatcgac gggttcgcgc gacaaggtaa catcggtcaa gagcatcagg
     1741 tgcagcgaga tgaacgcgcc caactcggtc ggggcgtttt cgggaattgc gctgcggagc
     1801 tgttccaact ctttgcgcgt ggctttgacg gcggcatcga aacgttcggc ttcggcatcg
     1861 gtgtccgcct ccgcaacatc atactgcggc acttcctccg taccgcgcgc aatcaggtgg
     1921 gcgcaaccga cggcaatgcc tttgcccgcc gccacgccgt gcagcacgat actcattatt
     1981 cgccctcgcc gaagtagccg ttgattaagt cggtcagggc gcgcatcgct tccgcctcgt
     2041 ccgcgccgtc cgtctccagt tcgatgaccg tacccttggc ggcggcgagc atcatcagcc
     2101 ccatgatgct tttgccgttg acgcggctgc cgtttttcgt tacccagact tcgctttgaa
     2161 actgagacgc ggtttgggtg aatttgctgg aggcacgggc gtgaagtccg agtttgttga
     2221 tgatttcgat ggattgtttg agcatttcga ttcccgtgtt atgtatatcg gcagcagacg
     2281 ccgtttaaaa tgttttcctg ccctgccgct tcttcagacg gcatcgccgc tgcgccggca
     2341 caccaaatct tcgggcgcgg acgtgatggc gaaaatgcct tttaccgccg cctccctgac
     2401 gcattcggta aaggcggcaa ggtcttccgc cgccggcgaa tattggacgg ccttaaccat
     2461 catcggcgcg ttcagcccgg tcaaaatcgc cgatttgttt tcgcgcacga ggcggcgggc
     2521 tgcattgcag ggggtcgcgc cgaaaatatc ggtcataatc agcacgccgt cgttgtcggg
     2581 aaactcctga agcgcggcaa tggcgttgtt gatgatgtcg tcttggtctt ccgtcggttg
     2641 cacgccgagt atgcggacgt tttcaggcag tccgcccgga aaaaaatgat gtgccagctt
     2701 gcggtaggct tcgcctatgg tttcgtgtgt gatgattaaa agccctatca tattatgcgt
     2761 cctgttcctc gttgtcctac cggcgtatgg gcgcgatgcc gtctgaaagg ccttcagacg
     2821 gcatcgcgtc cttattttcc gtccaatgcg taaatctcgc ccagattgcg ccagcagccc
     2881 gccgcatcca tgccgtaacc gaaaacataa cggttcggca catccagtcc gacataatcg
     2941 gctcggatag gcttgggttt gtcgatcagc ttgttggcga acaccgccgc acggcagctt
     3001 gccgcaccca tttccaaaag tttggcttga atggcggaca tcgtatgccc ttcgtccaaa
     3061 atatcgtcca gcacgacgac gtgcctgccc cggatttgtt ccgcatcggg catacgcttc
     3121 cagttgaacg cgccgccctc cagcttgtcg ccgtaacggg aaacgtgaac ataatcaaaa
     3181 tctaagggaa aacgcaacag cggcagcaac tgccccgtaa acaccaccgc gccgcccatc
     3241 acgggcagca gcagcggata tttgccgccc aaatcgcgcg taatctcgtc cgccactttt
     3301 tgcagtgcgg cacggcattg gccttggtcg aacaaaagat cggcgttttc aagcatcgcc
     3361 tgtgtttcaa ggcgtttggt ttctaaatcg gtcatatgtc ggaatcggtc ggtaaaagga
     3421 aaattataaa ccaaagtatc ggctgccgtc cgaaccgtcc tgctcagcgg tcggtacgca
     3481 cgcgcacaaa tgtggcaaat ttcggcgtac ctttctgcgt aaagccacgg tagcggtagg
     3541 taatcagcgt gccgattttg ggcgggttgt cgcggtcttt gtctttgaaa ccgctgccga
     3601 tgcggaattc gccgtgccgg tttttgcagc cgaccgcgcc caaccgtccg gcttttcgcc
     3661 ctttgccctc atagtgccgc gttaccgtgc attcgtcgtc gtattggctt ttcagcttca
     3721 ataattggct gctcctgccg ccgctgtaac gggattcggg ctgacgcagc atcacgcctt
     3781 cgccgccctg cgcttcgatt tgttttaaaa agtccatcgc gtgctgccgg tcgcgcactt
     3841 tgatttgcgg gatgatggta atcggcgcgt tcggatgcgt tttcagccat tgcgttgcga
     3901 ctgccaaacg ccggtagagg ttgccctgcg ccttgggtac atcgaaaacg tgcaggcgga
     3961 tgccgcgcca atctgaagaa gcagaacgca cggcggcgga aatctgctcg aactgaccgc
     4021 gtccgctata caattcgccg tccaaaggat aaggcggaaa ctgagcggta aaacctttgg
     4081 ggggcgtaaa cgcatagccc tgacggctta tcaggtgctt tccgtcccaa tacgcgcgca
     4141 cgccgtcgag tttctcgctc atcgcccagc cggcaatatc ctgccctttg tattcctgcg
     4201 ccagcatcaa atccgccgcg cctgccgatg cagggatgaa aaccgccata aaaatcggta
     4261 tgatgccgcc gattgtcttc ttaatcaccc gattccccca atatccaaac aggcggcaaa
     4321 ccgccataaa acaaacggca aacccgatgc cgtctgaaaa accgtttagg aacacgccga
     4381 tgaccctacg ttacgaaatc ttccccgtta cccccttccg ccaaaactgc accctgattt
     4441 gggacgacga aagtggcgaa gccgtcctga ccgatgtcgg cggcgatgtg ccgttcctgc
     4501 tgcaagcgtt ggcaaaccgc aaacttacgc tcacggcaat ctggctgacg cacggccatc
     4561 tcgatcacgc gggcggcgtg gtcgaaatgt tgaaaacgca taaagtccct gtcctcgggc
     4621 cgcatccgga cgatgaattc ctgctccaat cgctgccgca aaccaccgcg caatacggat
     4681 ttcccgtctc gcccgccttt gcgccgaacc gttggctcga agaaggcgaa acgctcacgg
     4741 tcggacgcta tgcctttcaa gtgctgcata ttccgggcca tacgccggga catatcgtct
     4801 tttattgtgc cgaggcggaa ttgctgattg cgggcgacgt gctgttttac gaaaccatag
     4861 gcagaaccga ttttccgcgc ggcaaccacg ccgacttaat caataatatc cgcaacaaat
     4921 tattcaccct tcccgaaacc gtgcaagttg tcgccggaca cgggcgtatg acttccatcg
     4981 gacacgaaaa gcggcacaat ccgtttttct aaccgccttc cctacggtct tcagacggca
     5041 tcatctgcac tgatgccgtc tgaaacgcaa aaggctcaga caaccgccgc cttgccggtg
     5101 tacctcgccg gacaaggctt tggtaagtac ttcaaacaaa ccccaaatca gaaccggtac
     5161 agttacgccg cgctttcggc attcccgccc ctgctgaaac aagcttttcc gcacaagtca
     5221 gactgcttca tcttctgcca catattccaa agattccgac aacgccgttg tttcattggc
     5281 acgttcggtc aaatcgcgtg caagctgctt gtttgccttc acacccaaca gacgcagttt
     5341 ctcggcgcgg ccgaccagat tcccgcgccc ttcggcaagt tgcttgaatg ccgtctgaaa
     5401 gctgttttgc gcctgatcga tgcctttgcc gacgctttcg agcgtctgta caaagccgac
     5461 aaatttgtcg tacagcttgc cgccttcgtc cgcaatcgcc agcgcgtttt ggttttgctg
     5521 ctcgttgcgc caaatattcg ccaccgtccg caaagtcgcc agcagcgtac tggggccgac
     5581 cagcataatc cgtttgtcga aacactcttg gaacaagccc gcgtcattct gcaacgccaa
     5641 caggtaggcc ggttcgacag ggataaacat aaagacgaaa tccaatgtgt tcacaccttc
     5701 caaatcggtg taatccttca gcgacaagcc tttcatgtgt gcacggatgc tggcaacgtg
     5761 tgccgccagt tcgcgtgccg ccgtatccgc atccgccgcc tgcgtgtagc gcacataagc
     5821 cgtcagcgaa accttggaat caatcacaat ctgcttgttg tcgggcaggt tgaccaaaac
     5881 gtcgggctgg aggcggcgcg tgccgccgtc ttcctctttt cggacggatg ccgcctgaac
     5941 cacatattcc cgccctttct gaaggccgga attttccaaa accgtttcca gaatcatctc
     6001 gccccaattg ccctgaactt tattctgcgt accggtcagc gcgttggtca gggcctttgc
     6061 ctcgctgtgc agctgcgcgt tcaacccctg aagccttttc aattcgtttt ccaacgtcag
     6121 ccgctcgcgc gattctttat cataggtttg cttgaccaac tcgccgaaac cgtggatgcg
     6181 ttcgtttagc gggttcaaaa cctgatggag ctgctcgcgg ttctgctcgg taaaacggcg
     6241 gcttttttct tccaaaatcg tgttggcaag attttgaaac tgatcgctca aactcttgcg
     6301 cgcctcgccc agtaaggaca gcttctcttc agaagcaagg cgttcctgtt cgatttgcgt
     6361 tgccaaacgt tcgttttcaa ccgccaaacc ctgtgccttt tcctgcaact cggtatgcga
     6421 ctgcctcaac cgctccgctt ccgcctcttt ttcctgcaaa tgggcaatct gtttttcggc
     6481 tgcggcaaaa cggttgccca catcggaaag gtcgttttgc acgtcgcgga cagtttggcg
     6541 gctttcttcc aaatcggttt cgattctttg gcggatttgg cgttccaagg catattggtc
     6601 ggcaaacgcc ttccgttcct gtccgagctg cgttgccaaa cgttcgtttt caaccgccaa
     6661 accctgtgcc ttttcctgca actcgatata cgactgcttc agccgcgccg actccgcctc
     6721 tttttcctgc aaatgggcaa tctgcttttc ggctgcggaa aaacggttgc ccaaagcata
     6781 attttcgtcc tgcaaatgcc ggtatttccc gtccaacacc gccaattccg acacggtttt
     6841 gccgtgtgcc tgttcgacaa aatcacatct tgccgccttt tccgccagtt gcgcgttcaa
     6901 atcggcaaac tcgccctgaa gcctgccctt catcagcaac catgtaaaca acacgcccga
     6961 caccaacgcc gccaaaggca gcaaaacagt catcagttcc atcaatcatc ctaatattca
     7021 aacattttca caccggacaa aaccgccgct tattccgatt ctacctgttt gttcgacata
     7081 gctccaaaaa atatagcgga ttggctttaa acctgttcga catcgcctta ccatgctgct
     7141 tgcggtttca gaccttttcc taattcaata tcaatctgcc acaaaccctg attaagttcc
     7201 cgatgtctga catttttaga atgatgccgt ctgaaatgtt gcagctatgt tcagacggca
     7261 tacggattca ggcttttcaa acggcaggca aaatgaaaaa agggcaaacc ctaaaggatt
     7321 tgcccttttg ttccaaacgc ttagtgtacg tctttccaat attctttttt caggaaatac
     7381 gccaaaggca gcataaccgc aaataggaaa atcatcacga catagcctat acgtttgcgt
     7441 tgcagttgtg caggttcgcc catgtacaca aggtaattga ccaaatcgcg tacatatgcg
     7501 tcgtactctt tttggatcac tttgccgtta ggcagacggc ggctgtgcaa accggtagat
     7561 tcccaataca gcttaggctt catctcgccg tgttcgtctt ttaccataac cggctgacct
     7621 ttggcatcca actcaacggc ttgaacgcct tgctgttccc acaacgggtg gggcataccg
     7681 actttatcga atacagtatt gttccagccg ctcggacggg tcggatcttt atagaagccg
     7741 cgcatatagg cgtaaaggta gtctgcacct ttggaacgcg caatcaacgt caaatcgggc
     7801 ggagcagcac caaaccattt tgccgcatct ttcgggttca tcgccgaatg catgacatcg
     7861 ccgacattat cggtggtaaa catcaggttt ttcttgattt cttcgtcagt caaaccgatg
     7921 tctttcaaac ggttgaaacg cataccgctt gccgagtggc aggacaggca atagttggta
     7981 aagatttgtg cgccgcgctg caggctgact tggtcgcgca aatcgatatc gactttctca
     8041 tagtgtgcat gaccgcccga agcaaatgcc gcacccatag gtactgccaa cagcaaggca
     8101 gcaaaccaat ttttcatagc ttgtttcatt ttgactgccc tcatcagatg ttggttgcaa
     8161 acaggtaagc acccacggcg gtaatgccga cgtaaacaaa gaacatgatt ttttgtttgg
     8221 cggtgctcat ggttacgcgt tcgggaaccg gtttgttttt atccagcttg gtatagaacg
     8281 gcatacccag gaagaatgcg aaatagacga aggacaggat gcgcgcgacc aaagtacgcg
     8341 tatcggttgc caccatcgcg cccaaaatac ccaaaccgat aaaggaaatg atgaacaaga
     8401 ccaatgcggt tttgaaaatc gggccgcggt aacggacgga tttcacctcg cccctatcca
     8461 gccaaggcag cagggcaatc aacacgaccg ccgcgcccat accgattaca ccccaaacct
     8521 gcgttcccaa gaacgaggga atcgcacgca aaatggcgta gaacggtgtg aaataccata
     8581 ccggcgcaat gtgcggaggt gttttcagcg catttgccgc atcgaagttc ggcgcttcga
     8641 ggaagtagcc gccgccttcc ggtgcgaaga acaatacggc acagaagaca atcaggaata
     8701 caaccacacc caagatgtct ttaacggtat agtaaggatg gaacgggata ccgtcgcgcg
     8761 ggataccgtt ttcgtctttg tttttcttga tttcaacgcc gtcagggttg ttagaaccca
     8821 cttcgtgcag ggcgatgatg tgcgccacaa ccaagccgag caataccaga ggtaccgcga
     8881 taacgtgcag ggcaaagaag cggttcaggg taacgtcgga aacgttgaag tcgccgcgaa
     8941 tccaagtgga caaatccgga ccgataacag ggatggcgga gaacaggtta ataattacct
     9001 gcgcacccca aaaggacatc tgaccccaag gcagcaggta gcccataaag gcttccgcca
     9061 tcaatgccaa gaaaatcaga gaaccgaaaa tccacaccaa ctcacggggt tttttgtacg
     9121 aaccgtaaat caaaccacgg aacatgtgca gataaacaac aatgaagaag aacgatgcgc
     9181 cggtggagtg catatagcgg ataatccaac cgccggacac gtcgcgcata atgtattcta
     9241 ctgcggtaaa ggcggcaggc agatggtagg cgttcaggtt gccgtcgggt ttgtagttca
     9301 tggtcaggaa aatgccgctg acgatttgaa tcacgaggac aagcagggcc aaagagccga
     9361 agtagtacca aaaattaaag tttttcggtg cgtagtattg agccaaatgc tcattccaca
     9421 ttttagacag ggggaaacga gcgtccatcc agcctaacaa tgcttttgct ttgctattgg
     9481 tttggtttgc cataattatc gttccttatt cttagtcttc gcccaccaag atagttgtgt
     9541 cgctcaagta tttatatggc gggacaacca ggttggtcgg ggcaggaaca cctttatata
     9601 cgcggccggc caagtcgaat ttcgaaccgt ggcaggggca gaagaagccg cctttccagt
     9661 ctgcacccaa atcggcaggg gcaatgtcgg gacggaaggt gggcgagcag cccaaatggg
     9721 tgcagatacc gatggcgaca aggatgttcg gcttaatcga acgggtctcg tttttagcat
     9781 actccggctg ctgttccgca tcggaattgg gatcggtaag ttcgccgttc aggcctttca
     9841 ggtctttaag ctgctgatct gtacggttga gcacccaaat cggtttgcct tgccactcgg
     9901 cggtcagcag ctgacccgct tcgattttac tgacatccac ctcgacggca gcaccggcgg
     9961 ccttggcttt ttccgaaggg aaaaaactgg ccacaaacgg cgttgccaca cccaatgctg
    10021 ccactccgcc cgcgccgcag gtcgcgagtg tcaggaaacg gcggcggccg ttgttgattt
    10081 cttgattatc cattattcag tcgtcctaat attttgggaa taccgagcca ttaaacgttg
    10141 caattttacc cagtttgcag tgatactcaa agcattattt aaaataaggt aaagttttat
    10201 gatatttctc aagactcaag ccggattgtt ttcgtcaaaa tggcacactt ccaacccgaa
    10261 aacctctgcc gccgattctg ccagcgcgcg tacgccgtaa cgttccgtcg cgtgatgccc
    10321 tgccgaaatg aaagccgtac ccgtttcatt ggcaaggtgg tattgggctt cagagatttc
    10381 ccccgtcaaa tacagatcga caccttcgtc tattgccgtc tgaaaaaacc cctgcgcccc
    10441 gccgctgcac catgcaaccc gtcggatttc gcgttcgggt ttgccgatga cgacaggctt
    10501 acgttgcaaa actgtttcaa tatgcgccgc caatgcgccg agtgtcttgg cttgtttcag
    10561 gctgcccgag ttgagcaggt tttgttcgcc gaaccgtttt tctgtcgcaa aacccaatct
    10621 gtcggcgagt tgggcattgt tgcccagtgt gggatgtgca tccaggggca gatggtagcc
    10681 tgccatattg atgtcgtgcc gtaacagtgc ggcaatccgt tcttttttcc aaccagtaac
    10741 ggtcggcaac tcgtttttcc agaacatacc gtgatgtacc aaaagcaaat ctgccttctg
    10801 ctccacagca aaatcaatcg ctgccctgct tgccgttacc gacgtaacga ttttcccgat
    10861 atattccctc ccttcaacct gcaaaccatt gggggcgtaa tctttaaacg atgccgtctg
    10921 caacgtttcg tcgcaccagg ctaaaaaatc tctgcgcaaa accatatttt ttcctgtcat
    10981 ttccaaacaa gcgggcattc taaccgcaaa tgcccgaaac gcccattttt ccgatttgca
    11041 cccgcatatg aattatttta atatgcgccg gttcaatatg ccgtctgaag cccccatgga
    11101 ttccattatc gaattgcgcc acctcaaaac cctgctggca cttgaagaaa ccggcagcgt
    11161 ctcccttgcc gccaaacggg ttttccttac ccaatccgcc ctttcccacc agatccgtat
    11221 gctcgaaaac cactacggca cgccgctgtt cgaacgcaaa tccacgccct tgcgctttac
    11281 cccggtgggc gaaaggctgc tgcgcctcgc ccacgaactg atacctcaag ttgctgttgc
    11341 agaacgagat ttggcgcgaa tcacagaagg ggaggcggga gagctgcgga ttgccgtcga
    11401 atgccatacc tgtttcgact ggctgatgcc cgccatgggc gaattccgcc cgatgtggcc
    11461 ccaagtcgaa ttggatatcg tatcgggatt ccaagcggat cccgtcggac tgctgctgca
    11521 acaccgtgcc gaccttgcca ttgtttccga agcggaaaaa caaaacggta tttctttcca
    11581 accgctgttt gcctacgaaa tggtcggcat ttgcgcacca gaccatccgc ttgccgccaa
    11641 aaacgtttgg acggcggaag actttatcgg ggaaaccctg attacttatc ccgttcccga
    11701 cgagatgctg gatttgccca aaaaaatcct gattccgaaa aacatcaacc cgccgcgccg
    11761 acacagcgaa ctgaccatcg ccattatcca actggttgcc agcagacgtg gcattgccgc
    11821 ccttccctat tggacagtca tgccctacct tgaaaaaggc tatgtcgtcc accgccaaat
    11881 tactgccgac ggactgcaaa gcaaactgta tgccgccatc cgtaccgaag atacggacaa
    11941 gagctatctg aacaattttt gccaaatcat acgcgaacgc ggttttgcag atttgcccgg
    12001 actgagcgaa ctggaaccgg tctgacccct tattcaacca tacccggcag tttttctatt
    12061 ttttcatgta tagtggatta acaaaaacca gtacggcgtt gcctcgcctt gccgtactat
    12121 ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atactgtttt
    12181 tgatttttgc ccaatctgta atctttagat tgccaatggg aaaccgtcta ctacaaataa
    12241 aaaaccctgc gataagcagg gttttttgaa tttccaacat taacgtttgg agaattgttt
    12301 tgcacggcgt gctttgcgca gacccggttt tttacgttcg acttcgcggg catcgcgagt
    12361 aacgaaaccg gcttgagaca aggcgggttt caacgcggca tcgaagtcga tcagggcacg
    12421 ggtaatgccg tggcggattg cgccggactg gccggtttcg ccgccgccaa caacattgac
    12481 tttgatgtcg aaagattcgg cgttttcagt cagaaccaag ggttggcgaa caaccattcg
    12541 gctggtttcg cgtgcgaaga attcgtcaac gggacggccg tttacgatga tttgacctgt
    12601 acctttaatc aggaatacac gagccactga acttttgcgg cggcctgtgc cgtagtagta
    12661 tttaccgttc atgtcgcgtc cttatttcag ttccaaaact ttgggttgtt gcgcagcatg
    12721 ggcgtgttcc gcacccgcat acactttcag ttttttaatc atggcgtaac ccaaaggacc
    12781 tttgggcagc atacctttta cagcttgttc caaagcgcgg cccgggaatt gctcttgcat
    12841 ttcgcggaaa gtgcgttcgt agataccgcc tgggaaaccg gaatggcgga agtatttttt
    12901 atcttcgaat ttggcaccgg ttacacgcag tttgtccgca ttgataacaa tgatgtaatc
    12961 gccggtatcg acgtgggggg tgtattcagg tttgtgtttg ccacgcagac ggctggcgac
    13021 ttcggccgca acgcgaccca agactttgtc ttgggcatcg atgacgaacc attcgcgctt
    13081 cacctcgtgg ggtttcgctg aaaaggtttt catagtggaa atccagatag atatagaaag
    13141 ttgtaaattt taaagacagg attcgatttt gtcaatcgca ttaccgcgtt acggaaggat
    13201 tttccggatt tcggcagact gcatactgct ttttcgggcg gggcggcggc caatgtgaaa
    13261 aaccgcatcg ttgcgatgcg gttttgaatg ggaatccccg cgagagccgt ttcggccgaa
    13321 tccgcttgaa ccttgctgac aaggcggctg cctcgggtag tttcgggtgc gtccgcaaaa
    13381 ggacgctcgc gcccactact gctcccggca accttaagcg aacttattgg ttcaaaggaa
    13441 tatatgcctt cgcggacacc gcagggaaaa aggggttatt cctgcgccaa gcgggatagt
    13501 gctttttggc aggcgttgtc catatcggct attttacgcg caaaatcgcc gattgccaaa
    13561 tcgccgccgt tcagggaggt tttcaacagg tcgtggacga cgttgagcgc ggccataatg
    13621 acgatttttt cgctgtccgc gacgcgtccg ccttcgcgga tggcttcggc tttgccgttg
    13681 agcattccga ctgcctgcaa cagtgtgtct ttttcttctg ccggcgtgtt gacggtcagc
    13741 cgggcgtgca tgacttcgat gtggacttgt tcgatgttca tcctttaatc cttattgctg
    13801 cgtttcctgc cgttggggga ggcgcgctgc cagtgcgctg attttttccc tgctctgttc
    13861 gagcaggctg cggtatcgtg tattttcttc tgtcaggctg tcaattttgt tttgcaggtc
    13921 ttctttgagt ttgccgactt ggacgagcag ggcttcgctg agttcgtcga cggcggtttc
    13981 gtgttcgagt ttttgccgct cgtgcgcccg tttgagttcg gcgacggttt ctttgaggcg
    14041 gcggttttcg ctgacgaggg tttcgaattt ttgtaccagc gtataaacgc tgctttcgag
    14101 tttttcgata ttttgtttca taaccttacc tgtccgtatg ccgtctgaag gcttcagacg
    14161 gcatctgtct gttgtttatt cgaaacgcgc gctgcgttcc atcagtcttt cgacgacctg
    14221 ctgaggggtc atttctttgc ggatgagttg cagcagggtt tgggtaatcg gcatgtcgat
    14281 ttggtactta caggcagtat tgaagacttc ttctatcgtg ctgacccctt cggaaacgtg
    14341 tccgatttcg accagcacct gatgcagttc cttgccttct gccaaaccca agccgacgcg
    14401 gcggttgcgt gagagtgcgc cggtgcaggt caagatgagg tcgccgatgc cagcaagccc
    14461 catcatggtt ttgggctgtg cgcccattgc ggaggcaagg cgggtgattt cagctaatcc
    14521 gcgtgtaacc agtgcggcac gggcgttaag cccgtactcc agaccgtcgg acaatccggt
    14581 ggcaatcgcc atgacgtttt tgactgcacc gccgactgcc acgccgataa cgtcggtact
    14641 gccgtaaagc ctcatgacgg tcgtgttgag ctgcggtacg agttcttcaa tccactcttg
    14701 gttttcggag gcgaggacga cggcgcaggg cagttgtttg gcgagttcct gtgcaaaact
    14761 cggaccggaa agtacgccga tttttttatt gtcgggcaat acttctttca atacttgaaa
    14821 ggtcagcagc cctgtatcct gctcgaatcc tttgcaggcg gcgaggacgg ggaggtgtcc
    14881 cgcgccgtac tgtttgagca gctctgcgct gcttctcaat ccggcaacgg aggttacgat
    14941 aaggacaagt ccgctgtctt tgagcgcgtc tgccaaatcc gcacacactt ccaaggtttc
    15001 gggaaaggaa aagccgggca gtccgcgttt gttttcacgc gcttcctgca tttggcggac
    15061 ttggtctgcg ttgcgcgtcc acagggatac gcggttgccg tgttgggaaa aatgcagggc
    15121 gagcgccgta ccccacgaac ctgcgccgat aacggtaatt ttcattggtc gtctttcaac
    15181 atatcactgc cgttcacttt aaaacaatcg gtgtttctct gcaagtgcgg tcagggaaat
    15241 gccgtctgaa aggcgttcag acggcatttt gccccgatgc ggcactatca gcctgtattg
    15301 cgcaaacctt gcgccacgcc gttgatggtc aggtgaacca tcagcagcgc gtgcggattg
    15361 tcgggttctt tacgcaggcg tttgagcatg gcgacctgca ggccgttgag cgcgttcaaa
    15421 tatggaatcc tcaaggcaag cgaacgggcg aggctgcggt tgtcgcgtaa aagttcttcg
    15481 gtttgcagca ggtcgagcag ggctttgcgg ctgcgttggt attcttcctt aatcatgccg
    15541 aagatgacgg cggctttttc cggcgattcg ctcaagccgg catagttttc cgcgagggtg
    15601 atgtcggttt tcgccatcac ttgctccata ttggaaagca tggcttggaa gaacgggttg
    15661 ctttgggcgt gttcgcgcag ggcggcgagc gtttcgggtt tgtcttcgca caaggtttcc
    15721 accgcgctgc cgaaaccgta ccaagccggc agcatgaggc ggttttgcat ccaggaaaat
    15781 acccacggaa tcgcgcgcaa gtcctgaatc cgcgccaagg ttttgcggct ggcgggacgg
    15841 ctgcctaggt tgagggtggc gatttcctga atcgggctgg tttgcagaaa gtagtcgatg
    15901 aagtcgggat gggtaatcag ttcgcggtag tatttgaacg atacgtccga caatgcctgc
    15961 atcagtttgg catcagggtc ttttttatcc ggcaggatgc tggcttccaa agtcgcggca
    16021 accagggttt ccaagttgcg ttgggcattg ccggggtcgg cgtatttggc ggtaatgact
    16081 tcgccttgtt cggtgatgcg gatttgtccc gccacgctgc ccgccggttg ggcgagaatg
    16141 gcttggtaag aagggccgcc gccgcgtccg acgctgccgc cgcggccgtg gaacaggcgc
    16201 atgcggacat cgtatttttt gaagagttcg accaagccca attccgcctg atacagacac
    16261 catgagctgg taacgtagcc gccgtcttta ttggagtcgg agtagccgag catgatttct
    16321 tggatattgc cgcggctttc gagcagggca tcgtaccagt cgaggcggaa catggtttcc
    16381 ataaccggac aggcgttttc caacgcttca atggtttcaa acagcggcac gatattgatg
    16441 cggctgcgcg gcttgccgtt ttccaccgcc aacaggccgc tttctttcag cagcaatgcc
    16501 aaggcaagca ggtcgctggg ttgttcgcag ttggaaataa tgctttgtgt aacggcatct
    16561 tcgccgaatt cgtctttgat tttgcgcgct tcgttaaaaa tcgccagttc gtggcgggta
    16621 tggtcgctgt atgtgatgaa cgggctgtac agcggacgtt gatggctcaa ttcgcgcaac
    16681 aggacggctt gtttttgctc ttcgttcagg ctgttgtagt cttccaagcc tgcgtgttgg
    16741 aaaagctcgg caaccacatc ggcgtgtttg cctgcgtgtt ggcgcaggtc gagcggcatc
    16801 atgtggaagc cgaacacgga cacggaacgg atgaggtctg ccaaacggcc ttcggcaagc
    16861 aggcggctgc cgttgtcgat aagggaacgt tgcaattttt tcaaatcatc gagaaattct
    16921 tgtgccgaag cataaggctc gaggaagccg aatttgcaac ccatacccaa accgagcgcg
    16981 cgcgctttgc ccatggcgcg cgccatgatg taggcgatgg cgcggcggta gggttcttcc
    17041 gtgcgggcga tttcttcgtc gggcgatttg tcggacaacg ccatcacatc gccgttgact
    17101 ttgacgcggc ggatggagag cggcagttcg cggtaaagtt tgtcgagttc gctgcggtaa
    17161 aaacggaaca cggcatcggc atgccgtctg aacgcaaagc gcagggtttc ggcagaaacg
    17221 aacggattgc cgtcgcggtc gccaccgatc cagccgccga ttttgaggat gttcggaacg
    17281 cggacatcgg gataggccgt ctgaaagtcg tgttccatct tgcggtagag tttgggcagg
    17341 gcttcaaaaa agctcatcgg gaagatggac acgccgttgt tgatttcgtc gttgacgctg
    17401 agtttgtggc ggcgcgtttc gctggtctgc cacaagccca gcaggatagt gtcgatttcg
    17461 cggcgcagcc gtgccagcgc gtcggcattg gtgcagcgtt cgcgttgcgg caacagtgcg
    17521 cggatgcggc ggttgaagct taagacggtt tggcgttgca cttcggtcgg gtgcgcggtc
    17581 aaaacggcgg taacggacgt attgtccaac tgccgctgca ccgatttgcc gtcggctttc
    17641 cccgctttga gcctgcggac ggtttccgtc aggctgcctt ccgcgccgcc gcgtccggct
    17701 tcttcgtgga tttggcggcg gcgttcgtgg tgcacgtctt cggcgatgtt caaaatctgg
    17761 gcgaacaggc cgcaggccaa ggttaaatcg tgggtttgtt gttcgtccaa ttgcggcaat
    17821 actttttcaa tcaatgccgc gctgtcgtcg gaagtggaca agagtttgac tgtttcgaca
    17881 accaacggcg aggcttcttc gtgcaggagg ttgaacaggg attgtttcag aaattccgcg
    17941 tccgccgcca aagccgcgtc cttcggattg ttcagaatat gcagttgcat gatttttctc
    18001 tcattgccgt aaatactgta aatgtacctc aaatgccgca tccgtgccaa accgttcaca
    18061 ctttaaccgc ccgtgtcccg aaatgccgtc tgaagttgaa cgccgcccga cggcagcgtt
    18121 acaatcgccc gcaactgttt tttccgaaca tcatcatgac cacgaccgaa cacgacaacg
    18181 acgacgaact cctgctgcgg tacagccgcc acatcctctt ggacgaaatc ggcatcgaag
    18241 ggcagcagaa actttccgcc gcgcatattt tggtcgtcgg ctgcggcggt ttgggtgccg
    18301 ccgccctgcc ctatctcgcc gcttccggca tcggcacgct gaccatagcc gattccgaca
    18361 cggtcgaact gcacaacctg caacgccaag tcgcatttga cgagggcgat gtcggcaagc
    18421 ccaaagccga agccttggca ggccgcctga aacgcatcaa ccataccgtc aacgtccaca
    18481 ccgtcaacga aaaactcgac ggctgccgcc tgaccgattt ggttcaagcc gccgacatcg
    18541 ttttagactg ctgcgacaac tacgccacgc ggcaagccgt caaccgtgcc tgcgtgcaaa
    18601 cgaaaacacc gctggtttca ggagcagcgg tgcgctttga agggcaactt gccgtgtacc
    18661 gtcccgactt gcccgactcg ccgtgttacg cctgcctgtt tgacggcgga tcggcttcag
    18721 acggcatctg ttccctcttc ggcgtattct cgccgctggt cggcatcatc ggcagtaccc
    18781 aagcggcgga ggctctgaaa atcctgctgg atgcgggcga accgtcgcac ggcaggctgg
    18841 cggtttaccg tgccttggaa gggggctggc aatatttcga cctgccgcgc aaccccgaat
    18901 gcccgatttg cggcacggaa cggtaaaacc ctgccgccgt ttcagacggc ataaagacgg
    18961 atgcggcgga acggttttaa aaatttaaaa atttacattt ctttgcaaaa aaaaaaaaaa
    19021 aaaaataaac ttaccttata attgcagttg tcttagcaat gtctgtttcg cagactcatt
    19081 gagtaagacg ttttccccgt aatgtgtttg gccgtctgtc ccctttgggt tcggacggct
    19141 tttttttggc tgtgtatgaa tacccggttg gttttatctg tttacagggg aaaaccgctt
    19201 atttccgttc gggcggaaaa cggttccatc ggataaaagg cattttgtcc gactgattaa
    19261 agttacatct taggtttgca tcggcaaaat attcaaacac agcctttttt aaggaaatcc
    19321 ggatacggcg gcacatcaat aatgcggcgg aatctcgtcg cgcagggaat acggctcttg
    19381 cgtgtcggga ttcctgtcct gcattttttg atacagcagc ctcaactgag cctgctgcaa
    19441 atccagcgtc tgccgcaatt ccgccaccat cgcgttcagg ccggcgatta cgtcctcctg
    19501 aagcgcggat tggatttcca gctctgtgat gcggtgttcc aactcttgaa ccgcgtccat
    19561 ttacagcacc atcgcggcaa tccagccggc aatcagcagc gggatattgt agtggatgaa
    19621 ggtcgggata acggaatcgc ggatgtggtc gtgctgcccg tcggcgttca gccccatcgt
    19681 cgggcccagc gtggaatcgg acgcaggcga accggcatcg cccaacgccc ccgccgtgcc
    19741 gacaatggcg acggtggcaa gcggcgaaaa acccaaaccg acacacaaag gcacataaat
    19801 cgcggcaata atcggcaaag tggaaaagga cgaaccgatg cccatcgtta ccaaaagccc
    19861 caccaccagc atcgccaatg ccgccatacc tttgctgttg ccgaatatcg ccatactgct
    19921 ttccaccagc ggctggatat gcccggtcgc attcatcacg gcggcaaaac cctgcgcggc
    19981 aatcataatg aagccgacca tcgccatcat cttgatacct tcgccgaata cgtcgtttgc
    20041 cttgtcgcgg ttgatgaccc ccaacatcat aaatacggcg aaaccgagca tcgcgcccaa
    20101 caccagcgag tcttcataca tcaactggat ggcaaaacat acggcaatgg cggcggcggc
    20161 ggccaggctg cggtaggcgg acggctgcgg acggtttgcc gcatcggcgt tgcccgccgt
    20221 atcggcattg ttgctttggt acaggcgcgg tttgcggtaa tggacaaacg ccagcaggag
    20281 tccggtcagc attcccaacg cgggaatcgc cattgccgcc attacgttga tgtttttcac
    20341 atcaagctgc ggcgcggcgg aatggatgtt gcccaacagg atttcgttca aaaaaatcgc
    20401 gccgaagccg taaggcagga acatataggt cgtaaccagc ccgaaagtaa tgacgcacgc
    20461 aatcagacgg cggtcgattt tcaggcggtt gaacaccaaa agcagcggcg gaacaatcat
    20521 cgggataaag gcgatgtgga taggtatgat gttctgactc ataatgccca tcacaaggat
    20581 gatggaaagc agcagccatt tgaccgcgcc ctcgcccgaa cgcacgctgt cgggcatacc
    20641 gccccggttc agcttgcgga cgaccgcgcc ggcaagctgc tgcggcaggc cggaatgggt
    20701 aatcgccatt gcaaacgcgc cgagcatcgc ataagaaagc gcaatcttcg caccgccttc
    20761 caaacctttg ttgaacacgg ggataatccc cgcctgactg acctgtcccg ccgcatcggc
    20821 aatgttttgc agcggcatac ccgccaccgc gccgccgaca aacgcgccga ccgtcaggct
    20881 caataccacg tgcacgcgcg acagcgacag caccagcata acgattacgg caactacgac
    20941 tgcattcatt gtttatcgct ccaaacctat aaatgtttac atatcgaaac acatcataac
    21001 ccaataatgg aaaacccgcc aattttgcaa acaattattt caaatgcttc atatacttcc
    21061 ccagcgtaac cctgtccaaa cccgccaagt ccggcagggt ttccactcct gaaaaaccat
    21121 tctccgccaa cacgccgcgc accgccgcgc cctgatcgaa accgtgttcc aacaacaaaa
    21181 aaccgccttc cgccaaacgg tcgggcgcgc cttgcgccaa ggtgcggatg cagcttaggc
    21241 cgtctgaaaa gtcggtcagc gcgatttgcg gctcaaaccg caaatcgcct tgcaacaaat
    21301 gtttatcgcc gttttcgata tagggcgggt tggacacgat gatgtcccat ttcccttcag
    21361 acggcatatc ggtgtcgaac cacgaaccgt gtgcaaattc gacccgcgcg cccaaatccg
    21421 ccgcattttt ccgcgccgtt tcaagggcgg gcgggctgat gtcggatgcg cgcacaaacg
    21481 catcggggcg ttcgagcgcg acggttacgg caaccgcgcc gctgcccgtc cccaaatccc
    21541 acacgcgccc gttttcgggc aggcgcgcca atacggcttc gaccaaatgt tcggtttcgg
    21601 ggcgcggaat cagcacgctc ggattgactg taaagcgtct gccataaaat tcgcgcacac
    21661 ctaaaatata ggcaaccggc tcgccgttca gacggcgttg cgccagcctg tccgcccgct
    21721 gtcggacttc gtccggcatt tcttccccgc cccgcgtcaa caactgcacg cgcgtatatt
    21781 ccgaaacata ttgcagcagc attcttgctt catttttagg cagttttgac aagcccaacc
    21841 atttatcaaa cgtcattttt cgtcccgtct gccgccgatg cggcttttct ttccttattc
    21901 tttccagcaa acgtaccgat ggaggcaacc gcaaatgcgg cataccacaa ataaaatcct
    21961 gcaccgtagc gcacaatatc cgatgtattc cctgcttcat cgacgtatac ggctttcaca
    22021 ctgaaagcca ccaacgccaa gccccaaaat gccgcatgga caggcacgac cttcttccgc
    22081 aacgccagca aaacaatggc cgccaaccaa acataattcg catagaccgc acaatacctg
    22141 atatccaaag aagcaaatat cgaccccaaa atcaaaacgg tcaaaccctc catgcttcca
    22201 tgattgccca aataaaatgc aacattggat aaagacgcta tccacagggc aaccgacacc
    22261 agcaacatca ctatgggaaa acttgatttc cgattctgtt cctgcatggt tttatcctaa
    22321 tgtaaaa
//
LOCUS       DAWHRA010000016        21329 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWHRA010000016 DAWHRA010000000
VERSION     DAWHRA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21329
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.016"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="WIB30_000458"
     CDS             complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="WIB30_000458"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ3796589.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAKADEGEYFGALFQYVGKDG
                     DVQDLQSVIGRLKAKGTIVAVSADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGIKVVHEVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSAYEDLVDLYRAFTGKDAFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHVVTDMHNASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVERGEWPKEDNPLVNAPHTASDVTGEWTHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3122..3481)
                     /locus_tag="WIB30_000459"
     CDS             complement(3122..3481)
                     /locus_tag="WIB30_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796590.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3429..3620
                     /locus_tag="WIB30_000460"
                     /pseudo
     CDS             3429..3620
                     /locus_tag="WIB30_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
     gene            complement(3756..4397)
                     /locus_tag="WIB30_000461"
     CDS             complement(3756..4397)
                     /locus_tag="WIB30_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ3796591.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4387..4938)
                     /locus_tag="WIB30_000462"
     CDS             complement(4387..4938)
                     /locus_tag="WIB30_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ3796592.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIDSDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4942..5460)
                     /locus_tag="WIB30_000463"
     CDS             complement(4942..5460)
                     /locus_tag="WIB30_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ3796593.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDNTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5460..7121)
                     /gene="pqiB"
                     /locus_tag="WIB30_000464"
     CDS             complement(5460..7121)
                     /gene="pqiB"
                     /locus_tag="WIB30_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ3796594.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATRGGGLDDLQAKVVDLLDKFDKLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLAGNPQTQNIPNELNQTLKELRQTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7114..8487)
                     /locus_tag="WIB30_000465"
     CDS             complement(7114..8487)
                     /locus_tag="WIB30_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ3796595.1"
                     /translation="MHPDWVYRLFSFIMPPLIFATFPMKPFAENIPHSLRGNCCDEAL
                     PPHTVDCPECGCRADVPRLDSGEAAFCPRCGHKLFRVGRHPFSAPPAYAAASLILMAF
                     AYGMTYIEVGIPGAASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVY
                     AALIRKQAYPALRLATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGPAFYLMF
                     ALSVMLIRTSVSVPQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDGAESPCGVC
                     GAELYRRRPKSLSISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDE
                     GDRLIAAVIFSASILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMI
                     DIFVIIILMCSFHTYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAF
                     NETEKHD"
     gene            8911..9540
                     /locus_tag="WIB30_000466"
     CDS             8911..9540
                     /locus_tag="WIB30_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ3796596.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGDKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGACEAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            9715..12091
                     /gene="hmbR"
                     /locus_tag="WIB30_000467"
                     /pseudo
     CDS             9715..12091
                     /gene="hmbR"
                     /locus_tag="WIB30_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
     gene            complement(12299..12421)
                     /locus_tag="WIB30_000468"
                     /pseudo
     CDS             complement(12299..12421)
                     /locus_tag="WIB30_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            12814..13062
                     /locus_tag="WIB30_000469"
     CDS             12814..13062
                     /locus_tag="WIB30_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ3796597.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            13059..13445
                     /locus_tag="WIB30_000470"
     CDS             13059..13445
                     /locus_tag="WIB30_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ3796598.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(13625..14980)
                     /gene="yegQ"
                     /locus_tag="WIB30_000471"
     CDS             complement(13625..14980)
                     /gene="yegQ"
                     /locus_tag="WIB30_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ3796599.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FAVSDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            15305..16093
                     /locus_tag="WIB30_000472"
     CDS             15305..16093
                     /locus_tag="WIB30_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ3796600.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAEAGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGWEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            complement(16139..16738)
                     /locus_tag="WIB30_000473"
     CDS             complement(16139..16738)
                     /locus_tag="WIB30_000473"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ3796601.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKLGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            16866..17483
                     /gene="gmk"
                     /locus_tag="WIB30_000474"
     CDS             16866..17483
                     /gene="gmk"
                     /locus_tag="WIB30_000474"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ3796602.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTGAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            17542..17748
                     /locus_tag="WIB30_000475"
     CDS             17542..17748
                     /locus_tag="WIB30_000475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ3796603.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            17840..19996
                     /locus_tag="WIB30_000476"
     CDS             17840..19996
                     /locus_tag="WIB30_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ3796604.1"
                     /translation="MPAPQPSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(20063..21247)
                     /gene="coaBC"
                     /locus_tag="WIB30_000477"
     CDS             complement(20063..21247)
                     /gene="coaBC"
                     /locus_tag="WIB30_000477"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ3796605.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILKGKKVLITAGATFEAIDPVRGITNISSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAENMHRAVHRLIDKQDAFISVAAVSD
                     YRVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMTYAR
                     EKRIKKKLPMIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMRIVERLAVYL
                     SK"
BASE COUNT         5179 a         5218 c         5785 g         5147 t
ORIGIN      
        1 tttgtccgtg tggaaatgac atcaaagggt gtgaataggc ttttcatctt tgaaatctgt
       61 ttgcacgatc ggatgaataa aaagtaggat gataaaaaag ctgatgtatg gatactggtt
      121 taactcattt gttttaaagt tatagtcgag atacgtgtat tggtgcaacc agtctgccgt
      181 cgacgaaaat gccgtctgaa agtgtttcag acggcatttg tctccggggt ttcaacggtc
      241 agtcttcata attttccatc ggtgggcagc tgcacacgag attgcggtcg ccgtacacgt
      301 cgtccacgcg gttgacgaag ggccaaaact tgtgttcgcg gacgaacggc aacgggaaga
      361 cggcttcttc gcgggaatac ggatgcgtcc attcgccagt tacgtcggat gcggtatgcg
      421 gagcgttcac caacgggttg tcctctttcg gccattcgcc gcgctcgact ttcaacactt
      481 cctgtttgat ttgtttcagg gcggcgatga agcggtcgag ttcggctttg ctctcgcttt
      541 cggtcggctc gatcatcagc gtgccggcaa cggggaagga gacggtcggg gcgtggaagc
      601 cgtagtccat caggcgtttg gcgatgtcgg tttcggtaat gccgctttcg gctttgagtg
      661 gacgcaagtc gacgatacat tcgtgcgcga cgcggccgtt tttgcctgta tacagaatcg
      721 gatagtcttc gctcaagcgt ttggcaacgt agttggcgtt gagcaatgcc cagcgtgttg
      781 cctgctccat gccttgtttg cccatcatgg tcaggtacat ccaagtaatc ggcaggatgg
      841 acgcagaacc gaaagctgcg gcagccacgg cagtttgatc ggcactggcg ttgtgcatat
      901 cggttaccac atggcctggt gcgaacggag ccagatgggc tttcaagcca atcggaccca
      961 tgcccggacc gccgccgccg tgagggatac agaaggtttt gtgcaggttc atgtgcaaca
     1021 cgtccgcgcc gacttcggca ggctgcatga tgccgatttg ggcgttgagg ttggcgccgt
     1081 ccatgtaaac ctgtccgccg ttttcatgga cgatgcggca gatgtcgcgg atgccttctt
     1141 cgtacacgcc gtgggtggac ggataggtaa tcatgatggc ggacaaagcg tcgcggtgtt
     1201 gctcggcttt ggctttcaaa tcgtcgatgt tgacgttgcc gtgttcgtcg gtatcgacga
     1261 cgacgacttt caaaccgagc atggcggcgg tggcggggtt ggtgccgtgg gcggatttgg
     1321 gaatcaggca gatgttgcgc tgcgcttcgc cttgcgcttc ctgatagcgg cggatggcaa
     1381 gcataccgct gtattcgccc tgcgcgccgg agttgggctg gaaggaaatt gcatcaaaac
     1441 cggtgatgga tttcaggctg ttttccatat cggcgagcaa ttcgcggtag ccggcggttt
     1501 gcgcttcagg agcgtaagga tggatgtcgg agaactccgc ccaagtaatc ggcaacattt
     1561 ccgcagtcgc gttgagtttc atggtgcagc tgcccaatga aatcatgctg cggttcatcg
     1621 ccaaatcgcg gtcttcgagt tttttcagat agcgcaacat ttcgtgttcg gtgtggtaac
     1681 ggttgaacac aggatgttgc agaatgtcat cttgacgcag caattcggcg ttcagacggc
     1741 ctttgacatc atcggcaaat gcgaacgcat ccttgccggt aaacgcgcgg tacaaatcaa
     1801 ccaaatcttc gtatgccgat gtttcatgga atgcagccgc aacttgagta tcgttgacgc
     1861 ggcgcaggtt gtaacccgac tctaaagcag cggcaaacac ttggtctgct ttctctttgt
     1921 tgccgaaatc gacggtaacg gtatcgaaga agacttcgtg aaccactttg atgccgtctg
     1981 aaaccagtgc atcggcaaag gcagaagcca gcgcgtgaat gcggttggcg atgcgtttca
     2041 cgccttcagg gccgtggtaa acggcataca taccggccaa attcgccagc aatgcctgcg
     2101 cggtacaaat attggacgtg gctttttcgc ggcggatgtg ttgctcgcgg gtggacaacg
     2161 ccatacgcaa ggcaggtttg cccgatgcgt ctttggatac accgatgata cggcccgggg
     2221 cggaacgttt gaactcgtct ttaaacgcga aataagcagc atgcggaccg ccgaagccca
     2281 tcggcacgcc gaagcgttgc gtgttgccca aagcaatatc tgcacccaat tcagccggag
     2341 acttcagcaa aaccaagctc atgatgtcgg cggaaacggc gacaatcgtg cctttggctt
     2401 tcagacggcc tataacgctt tgcaagtctt gcacgtcgcc gtctttgccg acgtattgga
     2461 acagcgcgcc gaagtattcg ccttcatccg ctttggcaaa atcgccgacc accaattcaa
     2521 agccgaaata cttggcgcgg gttttcatca cgtccaaagt ttgcggatac acgcgctcgt
     2581 ccacgaagaa acgctcggat tttaccttgc ccacgcggtg cgccatcgcc atcgcttcgg
     2641 cggcggcagt tgcctcgtcc agtaaagacg cgcccgccac agggaaaccg gtcaaatcga
     2701 tgcacacttg ttggaagttc aacaatgctt ccaaacgacc ctgcgcgatt tccgcctgat
     2761 acggcgtgta ggcggtatac cagccaggat tttccaatac gttgcgcaaa atcacattcg
     2821 gcacgcgggt cgggtaatag cctaaaccga tataggactt gttgatcacg tttttcgacg
     2881 caatgccttt caatttcgcc aatgcgtccg cctcggtcaa ggcctcgggc aaatcgagtt
     2941 cagacggcat acggatgctt tgcggcacgg tgttgccgac aaaatcgtcc atacttttct
     3001 cgccgacagc ggcaagcaac gccgcttcgt cgccgaaact caaatgccgc gcggcaaatt
     3061 cgttggggtt gaacagttcg gacagtttca tttctactcc ttcgggtttg tagggctgcc
     3121 atcaggcaga atttttagac atcggatgat tcgggcaagg gtagcacaaa agacgggata
     3181 atggcggaca atgcgccaga atgcagcagg tcaggacaag cagcgccaca agcggcagcg
     3241 ataagggaaa gccgccgccc aaaaccatga aacctgcagg caccagccaa gccaatggat
     3301 acgccggaag cggaaaaaca atattgagca gaaccaaaat cacgacaaac agcggcagac
     3361 ccgccatcag gttggcacgc ctgtatgttg cgtacgggtc ggatgcttct tcaggttcgc
     3421 aatattccat gatttcagcc tgtccgggct ttatccacag caggctcgga cggtcggtca
     3481 tgccgtctga accggcaggc cgtctttttc aaaaaacgcc ataccgctgc aacttcatgc
     3541 ggctgtatgg cgtaggcttt gcacttcaga cggcatatgc gtcccgaagc agtttggtgt
     3601 tccagtcggt tcatgattat agtggattaa atttaaacca gtacggcgtt gcctcgcctt
     3661 gccgtactat ttgtactgtt tgcggcttcg tcgccttgtc ctgatttttg ttaatccact
     3721 atatataaac aacttgggtg cggataaacc tttgtttatc taaacccctg ttttctcaaa
     3781 aattgattca aatcttcggc aaatttccga tagcctttgc cgttggcgtg gatttggtcg
     3841 gatttcagat tattatcgcc caaaatttcc gcccacgcgc cgccgaacag cggaatgccg
     3901 tattcctcgg acaaatcctc atacagcgga tgatcgctca aatgcccgaa caacgcaccc
     3961 agtgtgatgt gcggcacgcc gacgaggacg gcggggatgt tttccttctg cacggtttcg
     4021 atgattttcg cgatattggc gcgggtctgc tccttgggaa ctttgcgcag aaagtcgttg
     4081 ccgcctatgc cgacaatcac aagcttgggt ttgcgtgcca acagcgcggg caggcgcgac
     4141 agggcttggg cagatgtatc gcccgatacg ccgccgttga caatattcca acccgtcagt
     4201 ttttgcagtt gcgcggggta ggattcgcca gggtttgcgc cgtagccgaa ggtaagcgaa
     4261 tcgcccaagg caagtacggt gcttccttcg ggaattttgg cgtgggttcg ggcggatttt
     4321 ctgccgcagg cggtaagcag caacgcgcct gcgccgagga ggaaggttct tctgttcatt
     4381 ggtttttcag atggcatcga gccatttcgg gtggtatttc agggtttcac ggtaaaccgg
     4441 acagctttcg gcgtgcgcgc ctttgaggta gccggtactc atcagaaatt cgccgacgat
     4501 ttcgccgccg acgaatttga aatgtttttt aaagagttta acccattcgt ctttgcttcg
     4561 cggatggtgc gcgtcgagcc agttcttgaa cgagccgtat tcttgttgca acgcttggat
     4621 ttgccgtgca ttgaaaatgg cggcatcgat tttcaggcgg ttgcggacga tgcccgcgtc
     4681 ggcaagcagg cgttcgcggt cggtgtcgtc gaaggcggca accgtatcga tgtcgaaacc
     4741 ttcaaatgcc gtctgaaacg cctgccgctt tttcagcatc agcgtccagc ttaatcctgc
     4801 ctgattgatt tccaacacca gccgttcaaa cagtccgttg tcgctgtcaa tcggaaaacc
     4861 gtattgcgtg tcgtggtaat gtttgttcgg gttatcggtg ttttcgggaa gtgaggcggc
     4921 aaattcgcaa tagttcacgg tttactcgac catctgttgc gccgcctgtt tcagtccctg
     4981 ttcgagtgcg gcggtcatcg cggcgtagcc gtcaccctgc tgttcggttt cgatatggaa
     5041 gggtctgttc gtaccgtcgg gtaggacggc gtagccgctg atgagggttt tgcccgtgta
     5101 gctgccttgg aatgcgtcga tatagaccgt ccatttttcg gtactgccgc tgcgtgaggc
     5161 aggaacaaag atgcgtgtgt tgtccaaacg gttgaatgca ttgctcaacg ccgcttcgag
     5221 catatcgtcc aaggtgtctg cccaaacgtg gttttgtgcg gtgttgaggc ggtaggggtc
     5281 ggtttgatag accagtccgc cgcgtttgag cggttcggca agacggactt cgacggcggt
     5341 ttcgccgcct tgtgttgcag gacggatgta gcggctgtcg ggcaacacga aatattgtgt
     5401 gctttgcaca gtaccgcagg cggcaagcgt cagggcggcg gcaatcggga agaggcgcat
     5461 tatcggcttc ctttcgggat ggggtctttg ctgctgctgt tgaaaatcag cgcgttgggt
     5521 ttttctttca aagtgttaat gacgggttga acgtctttta aggttttgtc caaactttgc
     5581 aacgtatttt gtacgtcgcc gtagataggc gattgaggcg atacgccttg cagggtttga
     5641 cgtaattctt tcagggtttg gttcagttcg ttcggaatgt tttgcgtctg cggattgccg
     5701 gccagtttgt caatggagct tagggcggca ttggcagatt tgagtgcgga cttgagctcg
     5761 gcaagcgaac cgttcaattc ggcaaccgtc ttatccaaag gcagtttgtc gaacttgtcc
     5821 agcaaatcca ccactttcgc ctgcaaatcg tccaaaccgc cgccccgtgt ggcgatgacg
     5881 gtatcgcctg cataaacggt atgcggtcgc agcttgggcg aagacgaagg ctgatcgttc
     5941 aactcaatca ttttgctgcc ggtcagcagg ttgttgctgg agatggtggc ggtcaggcct
     6001 ttgtttaaag ccgtctgaaa ttgttgtttc caatgctctt tgctttgttc gtcggcattg
     6061 atttccaaac gggaaggctc gatgcggatg cgtacgggaa tccagccgtt ttcaaataaa
     6121 tgcaggctgt cgttgcggtc gaaataaggg acatcggaaa ccacgccgac attcagccct
     6181 ttgtactcga cgggcgaacc gacggtcagt ccgcgcacgg attgtttgaa aaacgcggtg
     6241 tagtacagcg agcggtcgtc aggcaggttg gcgacttcgc tgcggctgtc gtaaagcgtg
     6301 aagctgtctt cgcttttaac gtttttactg tttttggttt tcggcgaatc aaatgaaatc
     6361 gcgcccgaca gcagggcagg cagaggggcg gaattgagtt tgatgccgct gcctgtggtt
     6421 tcgatattga tgccgctttc cagccaaaaa cggctggcgg aatgaatcag tttgtcgttg
     6481 gggctttgga tgaagatggt gtaatgcacg ctttggtcgg acgggtcgaa atgcgcgctt
     6541 tcgacttgcc cgaccataaa gttttcatac aaaacagggc tgttgacgtt gaggatgcgg
     6601 tcgtttttac caatcaaatt caagcgcagc ccgctttgcc cgatggcggt aacgggcgga
     6661 atgtcctgca cttggaacac gtcttttgcc tcgtcgcttt tgccgggtgt aaaagcgatg
     6721 tacgaacccg aaagcagcgt acccaaaccg gttacgccgc tttggtcgat acgcggcttg
     6781 accacccaaa actgtgtgtc gctgcggatg aggccggata cgtccgcatt gagttgggcg
     6841 gttacttcca cgcctttctg gtcgtcgcgc agtttgattc gggtaacgcg tccgacatcg
     6901 atgctcaata ccttaatgac cgtattgttg acctcaatgc cttccgcgct gtccatcaag
     6961 agcgtaacca caggccccct gttgcggatt tccttaatcc acagccagcc accggcaatc
     7021 agcgcaatca gcgggaccag ccagacggcg gagagcaagg tgttgttttt gcggacgcgt
     7081 gcttgggcgt gtccgtttgg aggagggctg ttgtcagtca tgtttttccg tttcattgaa
     7141 agcaatgccg tctgaagcgc gtttgtccca aagcaggcgc gggtcgaaat aataggcgga
     7201 cagcatcgtc agaatcacga ccaggcagaa atagactgcc gcactgcccg gaatgacgcg
     7261 cgcggcataa gtgtggaacg aacacatcaa aataataatc acaaaaatat caatcatcga
     7321 ccagcggccg accgcttcgg tgatgcggta gaggtgcgac aatttctttg cgcccgttgg
     7381 caaagcgaag cgggcggacg caatcaaaac cgacattgcc gcaatcttca gtaccggcac
     7441 caaaatactc gcgctgaaaa taaccgccgc aatcagcctg tcgccctcgt cccacatata
     7501 agcgatgccg ttaaggatgg tattgacctc cgtggcggca ggattggacg aaatcataat
     7561 cggcaggata ttggcaggga aatacaaaat aaccgccgcc gtcagaaacg ccgacgaaat
     7621 actcagactt ttcggccgtc ggcggtacag ttccgcaccg cacacgccgc agggggattc
     7681 ggcaccgtct cggaaataca ggcagcggct gcaacaggtt ttgccttccg atgccgtctg
     7741 aaccgcatta tcccccgtca gccgcccgat ttgaaaatat acccaatgct ggggaacgga
     7801 taccgaagtc cgaatcagca taaccgacag cgcgaacatc agataaaacg ccggcccgaa
     7861 gcgaacctct gccacagacg agagcttgat atacgccacc aaagtggaaa caaaaaacac
     7921 atccaccatc atcgcctgcc tcaagcgcac catcacacgc gttgccaaac gcagcgcagg
     7981 atacgcctgt ttccgtatca gcgcggcata gacatacagg cacagcagca gaaacagaac
     8041 cggcgcgccg aaggtcagca caaacatcac ttcggccaaa aaaccataat cctgaaacac
     8101 catcaggcgc atcatctcgg gcagcgaaag gacggatgcc gcacccggta tcccgacctc
     8161 gatatacgtc ataccgtaag caaacgccat caaaatcagc gaagccgccg cataagcggg
     8221 cggggcggaa aaaggatgcc tgcccaccct gaagagtttg tgtccgcaac ggggacagaa
     8281 cgccgcttct ccactgtcca accggggtac atccgcgcgg cagccgcatt ccggacaatc
     8341 taccgtatgc ggcggcaggg cttcgtcgca gcagttgccg cgaaggctgt gggggatatt
     8401 ttcggcaaac ggcttcatcg ggaaagtggc aaatataagt ggcggcatta taaaggaaaa
     8461 aaggcgatac acccaatcag gatgcaaaac cggcaggttc ggcagtatga acaaaccctt
     8521 ggtgtccggc aaaaatattt tttgagcggt tgtccgccga ctgccgaacc catttgataa
     8581 aatcccgatt ctgtaaagaa tagtaaaata cctgcttaaa aacatcacgc aaatgatatt
     8641 ttttccaaaa atatgtgata ataaatttcg tttatgattc tttattattt ttatagtgga
     8701 ttaacaaaaa tcaggacaag gcggcgaagc cgcagacagt acaaatagta cggaaccgac
     8761 tcacttggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg aggcaacgcc
     8821 gtactggttt ttgttaatcc actatatatg cgggcaagtg cggattttgc ccgcaccgca
     8881 tccttatccc ttaccgacaa aggaaaaaat atgagtgaaa ccgaaaatca agcattgaca
     8941 tttgccaaac gcttgaaggc ggataccacg gcggttcacg acagcgtgga taacctcgtt
     9001 atgtctgtcc aaccgtttgt cagcaaagaa aactacatca aatttttgaa actccaatcc
     9061 gttttccaca aggctgtcga ccacatctat aaagatgccg aattaaacaa agccattccc
     9121 gagctggaat acatggcgcg atacgatgcc gtaacgcaag accttgcaga tttgggtgac
     9181 aaaccttacg aatacggcaa accgctgccg catgaaaccg gcaacaaagc aatcggctgg
     9241 ctttattgcg ccgaaggatc caatttgggc gcggcatttt tgttcaaaca cgcccaaaaa
     9301 ctcgattaca acggcgaaca cggcgcgcgc cacctcgcac cccatcccga cgggcgcggc
     9361 aaacactggc gcgctttcgt cgagcatctg aacgctttga acctgactcc cgaagccgaa
     9421 gcggaagcca tccaaggcgc gtgcgaagcc tttgcattct acaaagtcgt gttgcgcgaa
     9481 accttcggct tggcagccga tgccgaagca ccggaaggaa tgatgccgca caggcactaa
     9541 aaaataatcg aaccaaataa acaaggtctc ggcatagctg tttgcaggga cctttaatta
     9601 cacggcgcgg ctttgtttac atggattact gtcttattaa atattaatga ttatcataaa
     9661 atctattatt cgctaaccga tggatgaaca atccatacat cttgagttga taatatgaaa
     9721 ccattacaaa tgctccctat cgccgcgctg gtcggcagta ttttcggcaa tccggtcttg
     9781 gcagcagatg aagctgcaac tgaaaccaca cccgttaagg cagagataaa agcagtgcgc
     9841 gttaaaggtc agcgcaatgc gcctgcggct gtggaacgcg tcaaccttaa ccgtatcaaa
     9901 caagaaatga tacgcgacaa taaagacttg gtgcgctatt ccaccgatgt cggcttgagc
     9961 gacagcggcc gccatcaaaa aggctttgct gttcgcggcg tggaaggcaa ccgtgtcggc
    10021 gtgagcatag acggtgtaaa cctgcctgat tctgaagaaa actcgctgta cgcccgttat
    10081 ggcaacttca acagctcgcg tttgtctatc gaccccgaac tcgtgcgcaa catcgaaatc
    10141 gtgaagggcg cagactcttt caataccggc agtggtgcat tgggcggcgg tgtgaattac
    10201 caaacgctgc aaggccgtga tttgctgttg gacgacaggc aattcggcgt gatgatgaaa
    10261 aacggttaca gcacgcgtaa ccgtgaatgg acaaatactc tcggtttcgg tgtgagtaac
    10321 gaccgcgtgg atgctgcttt gctgtattcg caacgtcgcg gtcatgaaac cgaaagtgcg
    10381 ggaaaccgag gctatgctgt ggaaggggaa ggcagtggcg cgaatatccg tggttcggca
    10441 cgcggtatcc ctgattcgtc caaacacaaa taccacagct ttttgggtaa gattgcttac
    10501 caaattaacg ataaccaccg catcggcgca tcgcttaacg gccagcaggg acataattac
    10561 acggttgaag agtcttataa cctgaccgct tcttcctggc gcgaagccga tgacgtaaac
    10621 agacggcgca atgccaacct cttttacgaa tggatgcctg attcaaattg gttgtcgtct
    10681 ttgaaggcgg acttcgatta tcagaaaacc aaagtggcgg cggttaacaa caaaggctcg
    10741 ttcccgatgg attattccac ctggacgcgc aactataatc agaaggattt ggacgaaata
    10801 tacaaccgca gcatggacac ccgattcaaa cgttttactt tgcgtttgga cagccatccg
    10861 ttgcaactcg gggggggggc gacaccgcct gtcgtttaaa actttcgtca gccgccgtga
    10921 ttttgaaaac ctaaaccgcg acgattatta cttcagcggc cgtgttgttc gaaccaccag
    10981 cagtatccag catccggtga aaaccaccaa ctacggtttc tcactgtctg accaaattca
    11041 atggaacgac gtgttcagta gccgcgcagg tatccgttac gaccacacca aaatgacgcc
    11101 tcaggaattg aatgccgagt gtcatgcttg tgacaaaaca ccacctgcag ccaacactta
    11161 taaaggctgg agcggttttg tcggcttggc ggcgcaactg aatcaggctt ggcgtgtcgg
    11221 ttacgacatt acttccggct accgtgtccc caatgcgtcc gaagtgtatt tcacttacaa
    11281 ccacggttcg ggtaattggc tgcccaatcc caacctgaaa gccgagcgca gcaccaccca
    11341 caccctgtct ctgcaaggcc gcagcgaaaa aggcatgctg gatgccaacc tgtatcaaag
    11401 caattaccgc aatttcctgt ctgaagagca gaagctgacc accagcggca ctcccggctg
    11461 tactgaggaa aatgcttact acggtatatg cagcgacccc tacaaagaaa aactggattg
    11521 gcagatgaaa aatatcgaca aggccagaat ccgcggtatc gagctgacag gccgtctgaa
    11581 tgtggacaaa gtagcgtctt ttgttcctga gggttggaaa ctgttcggct cgctgggtta
    11641 tgcgaaaagc aaactgtcgg gcgacaacag cctgctgtcc acacagccgc tgaaagtgat
    11701 tgccggtatc gactatgaaa gtccgagcga aaaatggggc gtattctccc gcctgaccta
    11761 tctaggcgcg aaaaaggtca aagacgcgca atacaccgtt tatgaaaaca agggctgggg
    11821 tacgcctttg cagaaaaagg taaaagatta cccgtggctg aacaagtcgg cttatgtgtt
    11881 tgatatgtac ggcttctaca aaccggctaa aaacctgact ttgcgtgcag gcgtgtacaa
    11941 cctgttcaac cgcaaataca ccacttggga ttccctgcgc ggtttatata gctacagcac
    12001 caccaatgcg gtcgaccgcg atggcaaagg cttagaccgc taccgcgccc caggccgcaa
    12061 ttacgccgta tcgctggaat ggaagtttta atctggtatt attgaattaa tcgccttgtt
    12121 gaaaattaaa gccgtccgaa ttgtgttcaa gaattcattc ggacggcttt ttaccgtaaa
    12181 tctgtgtgat ggggttttat attgatacag tatccgaact caggcagggg gtaatgttgc
    12241 aggattaaaa atgccgtctg aaaaatgttt cagacggcat tttttggttg ccgatatgtt
    12301 aacggacgac gttggcgcgc atgaccacgc ggacgtggaa cgagcctgcc tgacagtcgt
    12361 attgtccgcc ttgcagcggc tgcttgtcga agtagctgtg gaagccggta acgccgcgtc
    12421 cgcaagggtg gcggcagatg ccgcattgtg tttataatgc cgtgtcagac gtttgattta
    12481 tgggaaaata atgatgctta ccaatcagga agtaaccggt gtcaaactgg gataccgcaa
    12541 agccgtttta tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca
    12601 gatagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagc
    12661 taaggcgagg caacgccgta ctggtttttg ttaatccact ataaatagca aggtgtgaat
    12721 aaaacggcgg atataatcaa atatatgtaa tgagattccc aaaatcttgg taatgatgta
    12781 tattagtcaa gttagctaat catgagaagt gttatgtttc aagcaaatat tcatcaggca
    12841 aaaaccaatt tgagtcaatt gattcaaaga gcagaagcag gggaaatcgt tattattgcg
    12901 aaggcaggta agccttgcgt ccaactaatc ggtattgaaa aaccggcacg aaatgcagga
    12961 aggttgaaaa aattcagtca tatggaaaat acggatattt cacgtattct tgaggatgac
    13021 aatgaaacgg cagctttatt ttttgaggag tcggctctgt gagaaagatt ctgcttgata
    13081 cccatgcgtt gctgtggtgg ttgttggatg acaagaaact ggggatatct gcacgcaaac
    13141 tgatagaaaa tccgagaaat gcgatctttg tcagtgcagc aagtatttgg gaaatttcca
    13201 tcaagcagaa caaggggttg ttgaaattac cggaagagtt ttttgatgtg cttcaagaag
    13261 aggattttga aatgttgcct ataggtctgt ttcatgcaaa acaggctgga agccttccgg
    13321 agatacacaa agaccctttc gataggatgt tgattgcaca aacccaagcg gaaggctttg
    13381 aactgatgac tgtcgatgaa tatattccgc aatatggaat tagagttgtc aacgcatcaa
    13441 gctgacaaat acaaaaactg tgtacacaag aaagaccgcc gaggcggtct tttcggttat
    13501 tgtcttcgct taaaagccag acttctgtcg tggactatag gtgtgtcccg atctgaatct
    13561 gccgcacaat aaggcaaggg gattaaaaat gccgtctgaa aaatgtttca gacggcataa
    13621 tggcttaggg gttcaacacg cgtgcgatga gggctttttc tttaccctgc atattgggga
    13681 ttttgacctg aatgccgttg cccggggcaa catcgacagg ctggcctttg cgggtcattt
    13741 gttccaattt gatggtttgg ttgccgctcg ggtggatgat ttcgagtgaa tcgctgacgg
    13801 caaagcggtt tttgacttcc actgttgccc agccgttttc atcgatttcg gtaacgtgtc
    13861 cgacgtattg gctttgtttg gcggtggaat ggccggtcag gtagttttga taatcctgag
    13921 tttggtggcg ttcgaggaag ccgctggtgt agccgcggtt ggcgaggcct tcgagttcgc
    13981 tcaacaggct gtaatcaaac ggacggcctg cgacggcatc atcaatcgct ttgcggtagg
    14041 actgggcgac gcgtgcaaca taatagagcg atttggtacg gccttcgact ttgaggctgt
    14101 ccacgccgat tttggcgagt ttttcgacga cttcgatacc gcgaaggtct ttggaattca
    14161 tgatgtaggt gccgtgttcg tcttccataa tcggcatcat ttcgcccggg cggttggatt
    14221 cttcaatcag gaaaactttg tcggcgtagg gatggcgttt ttgaccgttg atgccttcaa
    14281 agttttggtt ggcttcttct tgggcttttt caaagttgaa accttgcaga agctgggcat
    14341 cgcctgcatc gctttccgtg gcattgtgaa ccttgtaatc ccaacggcag gagttggtgc
    14401 aggtgccttg gttggggtcg cggtggttga aatagcccga caataggcaa cggcctgaat
    14461 aagcgatgca caatgcgccg tggatgaaga cttcgagttc gatgtcgggg cattcttggc
    14521 ggatttcggc gatttcttcc atactcaatt cgcgcgacag aataatgcgt tcgacgccga
    14581 tgttttgcca gaatttcacg ccccaatagt tggtggtgtt cgcctgtacg gacagatgga
    14641 tcggcatttc cggccatttt tcgcgcacgg tcataatcaa acccggatcc gccataatca
    14701 gcgcgtcggg tttcatggca atcagcggct ccatgtcggc aacgaaggtt ttgagtttgg
    14761 aattgtgcgg cagggtgttg acggttaaaa agaatttttt gttgcgctcg tgcgcttctt
    14821 taatgccttg ttctaaaaca tcaagtttgg caaattcgtt gttgcgggcg cgcagtgagt
    14881 aacgcgggct gccggcgtaa acggcgtctg cgccgtagtc gtaggcggcg cgcattcttt
    14941 ccaatccgcc ggcgggcaat aagagttcgg gtgctttcat cgtgtttcct tttcggttga
    15001 aaccccgccc tttagggcag tagaatcaga ctttatttgg gaggggcgta accccttccg
    15061 aatcagggca acatataggg cgacgcttta tgtgtcgtcc tgtgtgttga aacatgattt
    15121 gtatgctttg taaaaatgtt tcagacggca ttgctgcaaa tgccgtctga aaagggtttg
    15181 aggatgggcg gattatccgc ctttttgtat tttcggtcaa ttttgacggg aaatttataa
    15241 ggatttatca agtatttgcc tgcttgggat ataatgcggt agtttgaatg cgagagagaa
    15301 tgtgatgaat cctaaaatcg tgtttttcga cattgacgat acgctgtacc gaaaatatac
    15361 ggatactttg cgcccttccg tgaaaacggc ggtggcggct ttgcgcggca aaggtatatt
    15421 gacggcgttg gcaacggggc ggtctttggc gacgattccc gaaaaggtca gggatatgat
    15481 ggcggaagcg ggaatggatg ccgtggtaac gataaacgga cagtttgcgc tgctgcacgg
    15541 aaaaaccgtg cgcgaagtac cgatggatgc cggtttgatg ggcagggttt gcgcgcattt
    15601 ggatggcttg ggcatggatt atgcgtttgt cggcggagag gggatcgctg tgtccgcgct
    15661 gtcggaatgc gtgtgccgcg ccttgaagca tatcgccagc gatttttttg ccgataagga
    15721 ttatttttca agcaaaccgg tgtatcagat gctggtgttt gcggaggaaa acgaaatgcc
    15781 gctttggtcg gatattgtgg aacgggaagg cttgaaaacg gtgcgctggc acgaggaagc
    15841 ggtcgatctg ctgcctgcgg gtgcgtcgaa aacggacggc atcagaagcg tggttgaagc
    15901 attgggatgg gaaatggcag acgtgatggc gttcggcgac ggtttgaacg atgtggaaat
    15961 gctgtcagaa gtcgggttcg gcgtggcaat gggcaacggg gaacaggcgg cgaaagaagc
    16021 ggcgaaatat gtttgcccca gcgttgatga agacggcgtg ttgaggggct tgcaagattt
    16081 gggcgtgatt tgaacgcatc ataacaaccc tgccggtttc agacggcagg gtcggttttc
    16141 agcccttcat acagccttcg ttttgaagca gggtaaataa gggcgcgccg cttgcacgga
    16201 tattcttgcc gccttgaagg tcggtaaatt ccaaaatggc ggcggcttcg acaatttctc
    16261 cgccgagttt gcggatcagt tccagtccgg caagcatcgt gccgcccgtg gcaaccaaat
    16321 catcgaccag cagcacgcgc gaaccgagtt tgacggcatc ggtgtggatt tccaccgcag
    16381 cttccccgta ttcgagcgcg tagctttgcg atacggtttc aaaaggcagc ttgccttttt
    16441 tgcggatggg gacgaaaccg acgttgagct ggtaggcgag tgccgcgccg ataatgaagc
    16501 cgcgcgcgtc caaaccggca acgatgtcga ttttctgatc catatagcgg taaaccaata
    16561 aatcaaccaa aaggcggaag tattccgcgc tttgaaggac gggcgtgatg tcgtggaata
    16621 agatgccttt ttgcggccag ttttcgattt tgcggatttt gtcggcaagc gcgccgacac
    16681 tcatagcttc gggatgaacc agcattgcgt gttccaagtt tgatgttttt aacgccatat
    16741 tgtagccgtc agcctgtcgc ttgccaaatc aatcaaaaca atagcggcaa aatatgggca
    16801 tcggcaaata ggaaactggg tgtccattgt gatacaatcg gctggaaaat atacaggtaa
    16861 aaaatatgtc cgcttatcga aaaggcaaca tctttatcat ttcggccgct tccggcacgg
    16921 gcaaaaccac gctggtgtcg cggctgttgg caaaccataa cggtttgcgc gtttccgtgt
    16981 cgcacacgac gcgcccgccg cgtgaaggcg aagcaaacgg cgtacattat cactttgttt
    17041 ccaaagaaga gtttgagtcg cttatcgcgc aggaagcttt tttggaatac gccgacgtat
    17101 ttggcaacta ttacggcaca ggcgcggagg gtgtgaatgc gttggcggcg gcaggctatg
    17161 acgtgatttt ggaaatcgac gttcagggcg cggcgcaggt tcgcgacgcg ctgcccgaag
    17221 ccgtcggcat ctttatcctg ccgccttctt tcgacgtact tgccgcgcgc ctcaacggac
    17281 gcgggacgga cagtcgggaa gttatccaaa ggaggctgtc gaaggcaagg catgaaatcg
    17341 agcagtccgt attgtttgac tttgtcgtgg tcaatgacga cttggcgcga gcggaggagg
    17401 atttgcgcca tattgtgaat gcctgccgtc tgaaaaggtc gcggcaactg gggtttattg
    17461 cagatttgtt ggaaaattcc tagaaaacgg cgaaaatacc cggtttccca atttaaatat
    17521 ttttgaaaga aagcaaataa tatggcacgt attaccaccg aagactgtac cggaaaaatt
    17581 tccaaccatt ttgacctgac attggtagcg gctcgccgcg cccgccagct tgagaacggc
    17641 aacacgccgc ttgtggacga tgtccgcaat aacaaaccga ccgttaccgc cttaagggaa
    17701 atcgccgccg gacatatcgg tacagaactg ttgacgcgca ataaataaat tctgccggaa
    17761 acgcacgccg gaacactttg ccgccgtgca gtccgacggt ttgaaatgaa aaacacatac
    17821 ataccgaaag ccgtctgaaa tgcccgcccc ccaaccttcc gccccttacg accccctgac
    17881 cgccgaagcg cgtgccctgc ttttccatac cgcctcctac ctcaagcccg aggaacaggc
    17941 ggagcttgaa aaagctgtcg cctatgcgtt tcgcgcccac gacgggcaaa cccgcaaaag
    18001 cggggagccc tacatcacgc atccgattgc cgttgcgacg cagctcgccc tttggcatat
    18061 ggacatacag ggtctttgtg caggcgtgat gcacgacgta ttggaagata cgggcgtgac
    18121 aaaaggggaa atggcggcgg tgttcggcaa tacgattgcc gagatggtgg acggtctgtc
    18181 caagcttgaa aaactcaaat ttgaagatca tgcggagcat caggcggaga gtttccgcaa
    18241 actgattttg gcaatgacca aagatgtgcg cgtgattgtc gtcaaacttg ccgaccgcct
    18301 gcacaatatg cggacgctcg gttcgatgcg cccggacaaa cgccgccgga ttgcaaggga
    18361 aacccttgaa atctatgcac agattgccaa ccgtataggt ttgaataacg catatcaaga
    18421 gcttcaggat ttatcgttcc aaaacctgca tcccaaccgc tacgagactt taaaaaaagc
    18481 gatggacaag agccggaaga accggcagga cgttgtcggc aaagtcttgc gcgcattcgg
    18541 ccagcggctg gtaggcgcga atatagaggc caaaatcaaa ggcagggaaa aaaacctgta
    18601 cggcatccat cagaaaatga tggcgaaaaa gctgcgcttt gccgaggtta tggatattta
    18661 cggtttccgc gtcattgtca acagcattcc agcctgttat gccgcactcg gcgcattgca
    18721 caccctctat cagcccaagc ccgggcggtt caaagactat atcgccattc cgaaaagcaa
    18781 cgggtatcaa agtctgcata cgactttggt cggcccttac ggcttgccga ttgaagttca
    18841 gatacgtacc aaggaaatgg atgctgttgc cgaaggtgga atcgccggac attggagcta
    18901 taaatcatat tctaagacgg tcgatcaggc ggtgcttcac acaaaccggt ggctgaaaaa
    18961 tatcttagat ttgcaggcaa gcagtgccaa tgccattgag tttctcgaac acgtcaaagt
    19021 cgatttgttt ccgaacgaaa tctacatcct tacgccaaaa ggaaaaatcc taactttgcc
    19081 caaaggggca acgcctgtcg attttgctta tgcggtgcat accgatatcg ggcacaaaac
    19141 cgttgccgca cgtatcaaca atatcatgat gccgttgcgt acgaagctca aaaccggtga
    19201 ttctgttgaa attatcacat ccgaacacgc caaacccaat cccgcgtggt tgaatttcgc
    19261 cgtgtcaggc agggcgcgca gcgccatacg ccaatatatt aaaaacctta accggcacga
    19321 tgcggtcgtt ttgggagaga gcctcttaca aaaagccctg tccagtttgc tgcccaaaga
    19381 tgtcctgctt tcagacggca tcaaggaaaa atatcttgcc gatctcaacg acaagcagac
    19441 atcgtttgaa gaagtgctgt acaacgtagg gatggggcat accctgcctg tttatgtcgc
    19501 catgcacatt gccgagttgg caggggagca tttcggcagc gaggtcaggc tcagttccat
    19561 taaagtcgat gggcaggaaa gcgggcatat tcattttgca gagtgctgcc accctgttcc
    19621 cggcgattcc atccgtttgc tgttggttaa gggaaaaggc atgattatcc atagggatac
    19681 ctgcccgacg ttgttgaagt ccgatcccga acagcagctg gatgcagact gggaaaatat
    19741 gaacgggcag aactaccgtg tcgggcttca agtccaatcg gaagacagcc acggcctgtt
    19801 ggcattaatg gcgcaagcga tttccgattc cggtgcagac attgagtcgg tcgaaacacc
    19861 gtctaaatcc cagtcgggaa cggaaggttt tgtcgaattc aaattcttat tgaaagtcaa
    19921 gaatctgaat caattgaatc agattattca aaatctgcat agtattccat atatccgcaa
    19981 agtcatcaga agttgaagca ggtaaaacgg gttgcctttt gattaacagg caacccgttt
    20041 tatggtttat ccgttcaatt gcttatttgc tcaaatatac ggcaagcctt tcaacaatcc
    20101 gcattgccgc ttcatcttta cttgtttcgg gaaaagacag ttccccgtcg tccccgataa
    20161 tggtaatccg gttggtcggt ttgcccattg cgattgaaac atcattggca acgatcatcg
    20221 gtagcttttt cttaatacgt ttttcccgcg catatgtcat tacattctcc gtttcagcgg
    20281 caaaaccgat gcagaacggc gggttcggta atgaggcaat agaagccaaa atatcggggt
    20341 tctcatccaa ttcgatggat aacggtttgg catttttatc ttttttgaat ttttgagtac
    20401 tcctattctt aaccctatag tctgagacgg cggcaacaga aataaaagca tcttgtttgt
    20461 cgattaaacg atgcactgcg cgatgcatat tttcggcact gacggcttga accgtatcgg
    20521 atatgccgaa aggcagcgcg gtttgaagct gtccgtgaat caggctgact tctgcaccgg
    20581 cggcacggca cgcccgcgcc aaagccacgc ccattttccc gctggagata tttgtgatgc
    20641 ctcggacagg gtcaatggct tcaaatgtcg cacctgcggt aatcaagact tttttgccct
    20701 ttaaaatttt cggtgtccat aaatccggaa gcagatccag caattcggca ggttccggca
    20761 tccttcccat accattttct ccgcaagcct gttcgcccaa gcccggcata tagacagtaa
    20821 tgccgtctga aaccagttgt gcgatattcc gttggttggc agggttgagc cacatttcca
    20881 cattcatcgc gggcgcgatg gcaagcggac atttccgtgc ggctgccaga ctggtcagta
    20941 ggttatctgc cacgccgtta cagatttttg ccacggtatt catacttgcc ggcgcaatca
    21001 gaaaaacatc cgcattccgg gtcaggttga tatgttccat accgtttgaa ccgttgccgc
    21061 cgtgcgtgtc ggtcaggaca ggattgccgc ttaaagcctg aaaagtcagc ggagaaacaa
    21121 attcagttgc cgagcggctc ataaccaccg taaccgaatg cccctgtttt ttcagcagtc
    21181 gcaccaactc gcaagactta tacgccgcaa tactgcccgt tacacctaaa agaatatgtt
    21241 tgcccatttt gacaatttct aattacgaag aaaaaacagc agccattcta taacaaagta
    21301 cggatacatt agataccgtt tggatacaa
//
LOCUS       DAWHRA010000017        21267 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWHRA010000017 DAWHRA010000000
VERSION     DAWHRA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21267
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.017"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..891)
                     /gene="mafA"
                     /locus_tag="WIB30_000478"
     CDS             complement(<1..891)
                     /gene="mafA"
                     /locus_tag="WIB30_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ3796606.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVE"
     gene            complement(1075..1794)
                     /locus_tag="WIB30_000479"
     CDS             complement(1075..1794)
                     /locus_tag="WIB30_000479"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ3796607.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVITGEDEGTLVHC"
     gene            1887..1994
                     /locus_tag="WIB30_000480"
                     /pseudo
     CDS             1887..1994
                     /locus_tag="WIB30_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2116..2970)
                     /locus_tag="WIB30_000481"
     CDS             complement(2116..2970)
                     /locus_tag="WIB30_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ3796608.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(3099..3827)
                     /gene="rpsB"
                     /locus_tag="WIB30_000482"
     CDS             complement(3099..3827)
                     /gene="rpsB"
                     /locus_tag="WIB30_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ3796609.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(4004..4237)
                     /locus_tag="WIB30_000483"
     CDS             complement(4004..4237)
                     /locus_tag="WIB30_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796610.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            4315..4896
                     /locus_tag="WIB30_000484"
     CDS             4315..4896
                     /locus_tag="WIB30_000484"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ3796611.1"
                     /translation="MRNEEKHALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     AKLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVGTLPREAHDLPLDGFVSEAGILCF"
     gene            4957..5547
                     /locus_tag="WIB30_000485"
     CDS             4957..5547
                     /locus_tag="WIB30_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3796612.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHEMGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            6084..7550
                     /locus_tag="WIB30_000486"
     CDS             6084..7550
                     /locus_tag="WIB30_000486"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ3796613.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSTPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(7738..8112)
                     /locus_tag="WIB30_000487"
     CDS             complement(7738..8112)
                     /locus_tag="WIB30_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ3796614.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(8350..8649)
                     /locus_tag="WIB30_000488"
     CDS             complement(8350..8649)
                     /locus_tag="WIB30_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ3796615.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            complement(8804..9583)
                     /gene="map"
                     /locus_tag="WIB30_000489"
     CDS             complement(8804..9583)
                     /gene="map"
                     /locus_tag="WIB30_000489"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ3796616.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            complement(9623..10273)
                     /locus_tag="WIB30_000490"
     CDS             complement(9623..10273)
                     /locus_tag="WIB30_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796617.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPRPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKLHKEILDNLF"
     gene            complement(10281..10889)
                     /locus_tag="WIB30_000491"
     CDS             complement(10281..10889)
                     /locus_tag="WIB30_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ3796618.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGGAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLHQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(10953..11546)
                     /locus_tag="WIB30_000492"
     CDS             complement(10953..11546)
                     /locus_tag="WIB30_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ3796619.1"
                     /translation="MTTLQERVATHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPYPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(11551..11898)
                     /locus_tag="WIB30_000493"
     CDS             complement(11551..11898)
                     /locus_tag="WIB30_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ3796620.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQLNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            11944..12819
                     /gene="rsmI"
                     /locus_tag="WIB30_000494"
     CDS             11944..12819
                     /gene="rsmI"
                     /locus_tag="WIB30_000494"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ3796621.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(12854..13259)
                     /locus_tag="WIB30_000495"
                     /pseudo
     CDS             complement(12854..13259)
                     /locus_tag="WIB30_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(13378..13524)
                     /locus_tag="WIB30_000496"
                     /pseudo
     CDS             complement(13378..13524)
                     /locus_tag="WIB30_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            13766..14920
                     /gene="obgE"
                     /locus_tag="WIB30_000497"
     CDS             13766..14920
                     /gene="obgE"
                     /locus_tag="WIB30_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ3796622.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAACPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLIEKKRIEAEKAEAERAATNVEIAEQQPKTDTGVFKPE"
     gene            15003..16052
                     /locus_tag="WIB30_000498"
     CDS             15003..16052
                     /locus_tag="WIB30_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ3796623.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            16740..18161
                     /locus_tag="WIB30_000499"
     CDS             16740..18161
                     /locus_tag="WIB30_000499"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ3796624.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAAFDLSENANDYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            18244..19023
                     /gene="xth"
                     /locus_tag="WIB30_000500"
     CDS             18244..19023
                     /gene="xth"
                     /locus_tag="WIB30_000500"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ3796625.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            19010..19354
                     /locus_tag="WIB30_000501"
     CDS             19010..19354
                     /locus_tag="WIB30_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796626.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICMPCLLIASKCLGWKGWRLLANVLACLAVCALLSVPAA
                     LLIAFTLQDLLK"
     gene            19364..19894
                     /locus_tag="WIB30_000502"
     CDS             19364..19894
                     /locus_tag="WIB30_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ3796627.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(20123..21238)
                     /gene="asd"
                     /locus_tag="WIB30_000503"
     CDS             complement(20123..21238)
                     /gene="asd"
                     /locus_tag="WIB30_000503"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ3796628.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGAETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         4858 a         5177 c         5922 g         5310 t
ORIGIN      
        1 ctctacggat ggggcagagt tacccgtatg attgccgtat ggtcggatat cggagaaatc
       61 gaccattaat ccttccgtcg gtttaattcc tttgcttact ttatacggcc ccatccacaa
      121 tgcgtaattt tctttatagg cagcttcaaa cgcattggtt ttgggtttga tgagcaattt
      181 tttattggtt ctgtctactg cgaaatattc cagttttgtt tgggctttca gtgtttcggc
      241 attgtatagg tgcatttcgg ttctgttgcg tatcgttccg aatacgtcga tgttaataaa
      301 cacatccgta tcggcattgg caggagaaac aacgtctatg ccgcgcagga aaaatacggt
      361 ctgtaccaag tgggaaagaa aggcagtgtc gcgcgggtta gtcgtcaagg tttcatttcg
      421 ataatccccc atcccgccaa tatttaagcc cagactgctt ttacttccgc taccgtctga
      481 ttgggtgcgc gagagtgcag gggcattaag tgtagataaa gaagtggtta aacctgtcaa
      541 accgcctgat gttgtttcag cggtggtttc gtaacgtgga taggtgtaat cggtacggac
      601 ggcagggctg tttatgtatt cgccacgaat cagtgcatca atggagtagc gaccccctgt
      661 caaactgcct gaaccttggt cgcccatagt tgcaatgtac aatgcaactt ttcgtccgtg
      721 taatgcctgt aaatccatgt ctttaacggc agctctggca gaagcggcca caagttcttg
      781 ttcgaccgca aagcgtttac ctccgccatg cgatggaata cctgtcagtg tcccgcaggc
      841 ggataaaata aaaactgaaa aaagaatagg tatcagcagc cgtgcttgca tagattttct
      901 cctttgatga aaaacaaatt gtatcaaaat tgtaaatata gtggattaaa tttaaaccag
      961 tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa tccactataa tctccatata
     1021 ataaatggct gtctgaagcc catatgcgac tatatctgcc gacactatgg tcaatcagca
     1081 gtgaaccagc gttccctcgt cttcgccggt aatgacgcgt ttgagcgagc cttctttggc
     1141 gatgccgaag acgacgatat tgagcttgcg ttcgcggcag agggcgaaag cggtcgcgtc
     1201 catgactttg aggtttttca acaaggcttc gtcaaaagta atggtttcgt agcgcgtggc
     1261 ggacgggtct tttttcgggt ctgcggtgta cacaccgtcg acgttggtgg ctttgagcat
     1321 cacgtcgcag ttcatttccg caccgcgcaa tgcggcggca gtgtcggtcg tgaagaacgg
     1381 gttaccggta ccggcggcaa aaatcacgac tttgccttct tccaaatatt ggatggcttt
     1441 ggggcgggcg taggtttcag cgatttgctg catagacagt gcggattgta cgcgcgcttt
     1501 gatgcctaaa gtttcaaatg cgtctttgag tgccaacgcg ttcatcacgg tcgccatcat
     1561 gcccatgtag tcggcggtgg cgcgatccat gctgcctgct tgggcggata cgccccggaa
     1621 aatattgccg ccgccgacaa caataccgac ttgcacgccc attttaacga cttcggcaat
     1681 ttcgccgaca gtttgaacga tggtatcgtg attgatgccg aacggatcgg aacccatcag
     1741 ggattcgccg gagagtttca gtaatacgcg tttgtatttg atttgctgtg tcatggatac
     1801 cttgctttct tgaatgtcgt cggtcggagt tgggtcgtct gaatttcaaa cggtcggaat
     1861 tacaatgata tggcgtgtcg ttctgttata gtggattaaa tttaaattag gacaaggcga
     1921 cgaagccgca gacagtacaa atagtacggc aaggcgaggc aacgctgtac tggtttaaat
     1981 ttaatccact atattgtttt gttttcagac ggcctttata ttcggtcgtc ttaaaatagg
     2041 taccgtaaac ggttttctca aaaaaaagca ccctgattcg tttggaatcc aggtgctttc
     2101 tttttcataa gtgccttaca ctttggcggc tgcggctact tcggctgcgt agtcgacaac
     2161 ggctttctcg ataccgtcgc ctactttgta gcggacgaag ctgaccactt cagtgccgtt
     2221 ttctttggcg aattgggcaa cagtttgatc ggggttcatc acgaatgctt ggccgttcag
     2281 agtgatttca gccaagaatt tgcggatacg gccttcaacc attttagcgg cgatgtcggc
     2341 aggtttgccg gaagcgatgg cttgttcggt gtagatgtgg cgttcttttt caacggtttc
     2401 ggcatctact tcggcttcgc ttacgcattg tggtttagcg gcaacaatgt gcataccgat
     2461 tttgcgtgct acgtcttcag agcctttgta ctcaaccaat acgccttcgg tcgccaatgc
     2521 gccgtggatg taggcaacca gttggttggc agtgtcgatc acttggaagc ggcggacaga
     2581 catgttctcg cccaatttgg cgatgatggc tttgcgttct gcttcaacca gttcgctcag
     2641 ttcttcaaca gaagccggtt ttttctcggc agcagttttc gcaacgaagt tggcaaattc
     2701 tacgaagccc gcgtctttag caacgaagtc ggtttcgcag tttacttcga ccaatgcgcc
     2761 gacattgccg ttgatcgcgt aagccaatac gccttcggca gcggtacggc cggccagttt
     2821 accggctttc gcaccggatt tgatacgcag gatttcttcg gctttgtcga agttgccttc
     2881 ggcttcaacc aaggcttttt tgcattccat catgcccagg ccggtagcgg cgcgcaggtc
     2941 ggcaaccatt tttgcagtaa tttctgccat tttgaatctc ctagattttt gggaaatacg
     3001 gaaaggattt tccgcattcg ggaatcgtgt gccgcgaacg gacgttcaga cggcatattt
     3061 gagaaaaggg gcataacgcc cctcttcggt ttgccggatt actcggcagc ggcttcttgg
     3121 gcagcggcta cggtttcttg cagcgcttgg tttttgcctt ccaaaactgc gtcagcgatg
     3181 ccgcggcagt acaggcggat ggctttggcg gagtcgtcgt tgccggggat gacgtatttc
     3241 acgccgtcag ggctgttgtt ggtatcgact acggcgataa cggggatgcc cagtttttca
     3301 gcctcgacca gagtaccttt ttggtagccg gtatcgataa cgaaaatcgc gtcgggcagg
     3361 cctttcatgt ttttgatacc gcccaaagaa cgttccagtt tttcaacatc gcgttgcatt
     3421 tccagaattt cttttttgct gaaaccgctt tcggcagcat tttccaaggc tgcggttttt
     3481 tcttccaggc gtttgatgga ttgcttaacg gttttgtagt tggtcagcat accgcccaac
     3541 cagcggtaat cgacgaaagg cataccggcg cgggtcgctt cttcgcggat gatgtcgcgt
     3601 gcctggcgtt tggtacctac gaacaatact gtacctttgt tggcaaccaa acgacgtacg
     3661 gcttcttgcg cgtcttggaa catcggcagg gttttttcca ggttgacgat atggattttg
     3721 ttgcgcgcac cgaaaatgta ttgagccatt ttcgggttcc agaaacgggt ttggtggccg
     3781 aagtgaacgc cggcttcaat catctgacgc atagtaattt gagacatttt gatttccttg
     3841 aaagggttaa gggcacacat tcaatcgcat agaacttgat aggcgatgcc tattgtcaag
     3901 cacccttcgg cgaaagtggg cataagtttt aaaaaataaa agagtgtttc cgaaatggaa
     3961 aactgacgga ttatagcgga taagaggccg tctgaaaagg gattcagggt tttgtgccgc
     4021 tgtctttttg ccgttgtgca cgctgctgtt cgaattctgc ctgttcctgt tgcttcatca
     4081 agcggttgcg gcggcggacg gttacgacga agaccgtgaa cacggtgggc agcaccgccc
     4141 aaaaaaccaa ataaatcaag gcgcgcgcaa tactcggctg cgcggcggaa tacatcacgg
     4201 caacaaaaag atagccgatg gcgacaatgt gccacattgt gtgcgtcctg tttgaatgtt
     4261 aacaaaatcg ttataatcgc aacattctac cgcaatcctg aaaggccgtc tgaaatgagg
     4321 aacgaggaaa aacacgccct gcgccgcgaa ttacgcgggc ggcgttcgca aatggggtgg
     4381 gacgtgcggg cggcggcaac ggtaaaaatc aaccgcctgc tcaaacgtta tatcaagcac
     4441 ggtcggaaaa tcggcgtgta ttggccgatg ggcaaggaat tgcgtttgga cggctttgtc
     4501 cgcgcggcgc aaaaacgcgg cgcaaaactt tatctgcctt atatcgaacc gcgttcgcgg
     4561 cggatgtggt ttacgccgta tcctgaaagc ggaatggaac gggagcgcat acggggcagg
     4621 gcgaagttga acgtgccgca gtttgcaggg cgcaaaatcc gcgtgcacgg tttgtcggta
     4681 ttgctcgtcc cgcttgtcgg catagaccgc gaaggctacc gcttggggca ggcaggcggc
     4741 tattacgatg cgacgcttgc agcgatgaaa taccgtttgc aggcaaaaac cgtgggcgtg
     4801 ggctttgcct gccaactggt cggcacgctg ccgcgcgagg cgcacgacct gccgctggac
     4861 ggttttgtgt cggaggcggg gatattgtgt ttttagccgt tcagacggca ttccgggcgg
     4921 aaatgccgtc tgaaggcgga atattgggag aaaaggatgt cggaaaggat aatactgccg
     4981 gtcttgtcgt tgggcggcgt tcgtctcgaa ccgttggatg tgcatcatga aatgggtttg
     5041 cgcgaggcgg tttgcgatgg ggaagtttgg aagctgggag taacgtccgc gccccatccg
     5101 gatagggtgg cagactatat cgggacagca ttggcaacgc gtttggcgtt tgcggttgtc
     5161 gatgaagagg cgggcagggt ggtcgggaca acggcgtatt atcattttga accgcagatt
     5221 ccgcgtttgg atatcggatt cacatggtat gcggcatcgg cacggcggac acggataaac
     5281 acctgttgca aaatcatgct gttggattac gcatttgatg ttttggcttg ccgttgcgtg
     5341 ggatggcgca cggatattct gaaccttgcc tcgcaacgtg ccatcgaacg gctgggtgcg
     5401 gaaaaagacg gcgtgctgcg tatgcatatg ctccgtaaag acggcagtgt gcgcgatacg
     5461 gtcgtgtaca gtatgctgag ggaagattgg tgcaaaaaca gggaaatcct gaccggcagg
     5521 ttggcagggt atggcgtgca ggtgtgaggc agtattttca aatttatagt ggattaacaa
     5581 aaaccagtac ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctgaag
     5641 caccaagtga atcggttccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
     5701 tttttgttaa tccactataa aaaacttaac gcaatatgct gacaatcttt taaaaattaa
     5761 tatataaaca attttggttt tttatctatt aaaaatttga tatatataga taaataacta
     5821 tttttatttt tcatttcgca gttcccgggc ttggcgggcg acagaaaaac tttgcatttc
     5881 ccagtaaaat ccactacatt cacgataaca tattacaagc ccctgaagcg gtgtcggaag
     5941 ccatacttgc aacgcattcc aaccccgaaa cagcatactc ggacacacac cattgccggt
     6001 tttccctgct tttgcgattc aggcgtttgt agtacggcaa ataaccataa gggcagaaga
     6061 tgcctgtttt ttaggagtaa accatggctg aagcgacaga cgttgtcttg gtgggcggcg
     6121 gcattatgag cgcgactttg ggcgttttgc tcaaagaact cgaaccgtct tgggaaatca
     6181 ccctgattga acgcttggaa gatgtggcgt tggaatcgtc aaacgcgtgg aacaacgccg
     6241 gcacggggca ttccgcgctg tgcgaattga actatgcgcc gttgggtgca aatgggatta
     6301 tcgatccggc gcgcgccctc aatattgccg aacagtttca tgtcagccgc cagttttggg
     6361 cgacgttggt cgcggaaggc aagttggaag acaattcctt catcaatgcc gtgccgcata
     6421 tgtctttggt gatgaatgaa gaccattgtt cttatcttca aaaacgttat gacgcgttta
     6481 aaacccaaaa actttttgaa aatatggaat tttccaccga tcggaacaaa atttccgatt
     6541 gggctccgct gatgatgcgc ggccgggacg aaaaccaacc cgtcgccgcc aactactccg
     6601 ccgaaggcac ggatgtcgat ttcggacggc tgacgcgcca aatggtgaaa tatttgcagg
     6661 gcaagggcgt aaaaaccgag ttcaaccgcc acgtcgaaga catcaaacgc gaatccgacg
     6721 gcgcgtgggt gctcaaaacc gccgataccc gcaaccccga cgggcagctc accctccgta
     6781 cccgcttcct cttcctcggc gcgggcggcg gcgcgctgac cctgctgcaa aaatccggca
     6841 tccccgaagg caaaggctac ggcggcttcc ccgtgtccgg cctgttcttc cgcaacagca
     6901 cccctgaaac cgccgaacaa cacaacgcca aagtgtacgg gcaggcttcc gtcggcgcgc
     6961 cgccgatgtc cgtcccgcac ctcgacacac gcaacgtgga cggcaaacgc caccttatgt
     7021 tcggccctta cgcaggcttc cgttccaact tcctcaagca aggctcgctt atggatttgc
     7081 cgctgtccat ccatatggac aacctctatc ctatgctgtg cgccggctgg gcgaatatgc
     7141 cgctgaccaa atacctgctg ggcgaattgc gtaaaaccaa agaagaacgc ttcgcctccc
     7201 tgctggaata ctaccccgag gcaaaccccg acgactggga actcatcacc gcagggcaac
     7261 gcgtccaaat cattaaaaaa gactccgaaa aaggcggcgt gctccagttt ggtacggaga
     7321 ttgtcgccca cgccgacggc tcactcgccg cattgctggg cgcgtcgccg ggcgcatcga
     7381 ctgctgtgcc gctgatgatc cggttgatgc accaatgctt ccccgagcgc gccccgtctt
     7441 gggaaggccg tctgaaagag ctggtaccgg gttacggcat caagttgaac gaaaaccccg
     7501 aaagggcgga tgaaattatc gcctataccg cgaaagtgtt ggatatttaa gccgaatctt
     7561 tcaagaaaag ccgtaccccc atatggtgtt gatgccgtcc ctgccggtta tgcccttcat
     7621 ccggcagggc aggttttgat acacggggca ggtaatcgtt ttttcccttt tgcgaataat
     7681 ggaacggtca aaaaaacagc gttttcattt ctgaaaacgc tgttttttgt tgcctgatta
     7741 acgtggggaa cagtctttga agacgatttg gttgtcaggt gcggtaatca taatgggctg
     7801 tttgcggtaa gatttgccat ccatcacgcc ggtacccaaa acataaccgc cttctttgcc
     7861 gtagaatgtt tccacattgt cggatttatc caaattgaca ggcatttgca cacgtttgcc
     7921 gttgatgacg gcggaagcgt atgtggtcag accctgtttg ttgaagccgt aggttacttt
     7981 gactttttta ccttgctggc agacgtagct gacggttttt ttggcaacgg tggggttgtt
     8041 cgtgccggca gcagcagcca tactgctgag cgcgattgcg gaagacagga ttgcggtggt
     8101 cagaagtttc atttttattc ctttggatgg atgtggagtt tgcccgtttc gagctgtttg
     8161 acattctaat gtcggcgtat cctgaaataa acaggaaatt ggataaagtt tgtaaaaagg
     8221 cgggttcggg cggttcggcg gtttgaggca ggggcggcag gcgggatacg gcggcctgtc
     8281 cggtgtttca gacggcattg tttgcgtttt tcggaaatga aaaactgtcg ggcatccgga
     8341 ggttgcggtt taactttgtt tttttctttc ggacttggtc tgtttggaca attcggcata
     8401 caggtcggga tggtgttttt tcaacagggc ggcgatttcc atatcttttt tgttgacttt
     8461 cgccaaatcg atgcattcga tatggacgag gcggagcagt tcgcgtacgg gtttgggcat
     8521 tttgcggccg gtttcgtagc gcgatcctcc ggactgggta acgccgacct tgccccagaa
     8581 gtctatctgg ttcagcccgg tctttttacg gatggcttta acatcttcta tattttcaaa
     8641 tattttcata aaacttcctt taagtttgtc tggttgcggg gcaaattccg atttgctttg
     8701 ttttcgtaat gtgccgggtc aagcctgttt ttatattata tatcatatct gccgtttctt
     8761 tcagacggca ttgtttgttt ttatggggcg gatacgtccg gtttcagggt ttgccggagg
     8821 cggggctgac ggtgaggatt tcgtagccgg tttcggtcac caaaacttcg tgttcccatt
     8881 gggcggagag ggagcggtct ttggtaacca ccgtccagcc gtcgttgagg atacgcaggt
     8941 ggcgtttgcc ttggttgatc atcggttcga cggtaaaaat catacccggt tttagaacgg
     9001 ggccctgccc tttttttccg tagtgcaaca cttgcggggc ttcgtggaaa ccgcgcccga
     9061 tgccgtgtcc gcagaattcc tgtacgacgg aatagccggc gttttcggca acctgttggc
     9121 aggcgtagcc tacgtcgccc agtgtcgcgc cgggtttgac ggcttctatg cccgccatca
     9181 tggaggcgtg ggttacgtcg atcagccgtt gggcgatggg ggagactttg ccgacggtaa
     9241 acatacggct ggagtcgccg tggaagccgt cttttttgat ggtgaggtcg atgttgataa
     9301 tgtcaccttc tttgagcggc ttgtcgtcgg gaatgccgtg gcagatgacg tggttgacgg
     9361 aggtgcagca ggatttgggg tagggcgggt tgccgtagtg caggggggcg ggataaccgc
     9421 cttggacgtt gacgtggtag tcgtaaacga gtttgtcgat ttcgtcggtg gttacgccgg
     9481 gtttgacgaa ttgtccgatg tagtcgaggg cttcggcgac gagtttgccc agctcgcgca
     9541 ttttttcgat ttcttcgggg gttttgatga tgatgccgtt cataatgctt ccttggattg
     9601 cgggtgtgcc tgccgtgccg ggtcagaaga ggttgtcgag aatttcttta tggagtttag
     9661 gcggcgtgtt tttcggggtg tccggtttgg tatggttttc tttgggcgtt tctttttctt
     9721 tgggtgtttc tttgggctgc gcctgttttt cgggtgtttc ttttcgaggt tcgggacggg
     9781 ggacgtttgt aggcagtacg ggggtttctt tgagtgtgtt ttcagcttgt gttccggcgt
     9841 cgtccggctg tctgtcggtt tgagctgtgt cggcagggcg cggtttgacc cggtttttct
     9901 tgggtgcggc aggggacggc agtccgctgc cgctttcgtc tgtgccgtcc ggctgtgcgg
     9961 gttcggatga ggcggcgtgg tggtgttcgg gttgggcggc atcttgtccg actacgccgt
    10021 ttggcagcca gaattcggtt tcgcgggggc tgtcggtgtg ttgcggttcg gtttggaggg
    10081 ttttgtcgcc gttcgggttg aatgtgctga cgatgcctat tgccaatgcg ccgatggcgg
    10141 tgatacagat gagtaatacg gcgcgtatca ggagtttggg ggtcagcctg aagggtttgt
    10201 tcggtttttt tgccattttg attgtgcttt tggggtgtcg ggcaatgccg tctgaaggcg
    10261 gttcagacgg cattgccgag tcagcgttgg acgtagtttt ggtagagggt gatgactttt
    10321 tgtacgccga cggtggtgct gactttttgg gtaatctgcg cctgttcttc gggggtgagg
    10381 atgcccataa cgtaggttac gttgccgtag gtaacgattt tgacgcgcgc ctgtgtggcg
    10441 gggctgatgc ccaacagcgt ggcgcggact ttggatgtgt tccaagtgtc gccggcgatg
    10501 tcgccggcag tgcgcggcag ggaggcgacg gtaatgtagt tgtacacgcc ttcggcggcc
    10561 tgttcggaac gtgcaatctg accgacgaac tgtttctcgc cttcggtggc gacttgtccg
    10621 agcagcagca ggtggcggtt gtagccgacg acggagattt ggggcgtgta gcctttggtt
    10681 tggttgtttt ggtgcaggta ggaacgggcg gtggtttcga tacgcaacgc cataacgttg
    10741 tcgtcggttt gcgcgccggt ggttcggcgg tcgacggcgg atttcgcgcc gaccgccgcc
    10801 ccgccgatta ctgcgctgac gcagccgcta agggcaaggc tgaaaatggc ggcaatcagg
    10861 gtgcggacgg tgtgcggttt gggtttcatc gggggcttcc tttcttgggc gtttcagacg
    10921 gcattgcttt gcgccatgcc gtctgaaaag ggttacattc cttccagcag tacggagtcg
    10981 atacagtcgc acatggcgtg tatcagcagg atgtggtttt cctgaatacg ggcagttcga
    11041 ggatagggaa cgttgagtaa aacgtcggtg tctttgagta tggcggcgat tttgccgccg
    11101 tcgcggccgg tcagggcgat gacgtgcata tcgcgttcgt gtgcggcttt gacggcttcg
    11161 atgacgttgg cggaattgcc ggaagtggaa atgccgacga gaacgtcgcc ggcgcgtccg
    11221 agggcgcgta cttgtttgct gaagacgtgg tcgaagccgt agtcattgcc gatggcggtc
    11281 agcgcggagg tgtctgtggt cagggcgacg gcggcgagtt ccatacgttc tttttcaaaa
    11341 cgcccggtca tttcggcggc gaagtgctgc gcgtcggcgg ccgaaccgcc gttgccgcac
    11401 gccagaatct tgccgtcgtt catcaggcat tgcagcatca gctcggcagc ctgtacggtc
    11461 ggctcgacca atacttttcc ggcttcctgc ttggcacgga tgctttcggc aaaatgggtg
    11521 gcaacgcgtt cttgtaatgt cgtcatatcg tcaacctgta atattctgta tccactcggg
    11581 cgggcggttt ccttcgataa gtaccgcatc gaggcggcac ggtacgtttg tcagcctgtt
    11641 cagttgcaga taatactcta cacttcgttg cagtttcaat aatttggatg gggaaatgct
    11701 gtatgcgaca ccgccgaatt gccgattttt gcggtatttt acttcaacaa acagaatcat
    11761 gccgccgttt ttgacaatca ggtcgatttc gccgtaggcg cagtgccagt tgcgggcaag
    11821 cagcgtgcag ccttgggatt ggaggaaggc aagcgcggca tcttcccctg cctcgccctg
    11881 tttgtggttt aggcgcataa tcggtttaac ctttataatg tttgtttttc agacggcatt
    11941 cttatgtttc agaaacattt gcagaaagcc tccgacagcg tcgtcggagg gacattatac
    12001 gtggttgcca cgcccatcgg caatttggcg gacattaccc tgcgcgcttt ggcggtattg
    12061 caaaaggcgg acatcatctg tgccgaagac acgcgcgtta ccgcacagct tttgagcgcg
    12121 tacggcattc agggcaaact cgtcagtgtg cgcgaacaca acgaacggca gatggcggac
    12181 aagattgtcg gctatctttc agacggcatg gttgtggcac aggtttccga tgcgggtacg
    12241 ccggccgtgt gcgacccggg cgcgaaactc gcccgccgcg tgcgtgaggc cgggtttaaa
    12301 gtcgttcccg tcgtgggcgc aagcgcggtg atggcggctt tgagcgtggc cggtgtggaa
    12361 ggatccgatt tttatttcaa cggttttgta ccgccgaaat cgggagaacg caggaaactg
    12421 tttgccaaat gggtgcgggc ggcgtttcct atcgtcatgt ttgaaacgcc gcaccgcatc
    12481 ggtgcgacgc ttgccgatat ggcggaactg ttccccgaac gccgattaat gctggcgcgc
    12541 gaaattacga aaacgtttga aacgttctta agcggcacgg ttggggaaat tcagacggca
    12601 ttgtctgccg acggcaacca atcgcgcggc gagatggtgt tggtgcttta tccggcgcag
    12661 gatgaaaaac acgaaggctt gtccgagtcc gcgcaaaaca tcatgaaaat cctcacagcc
    12721 gagctgccga ccaaacaggc ggcggagctt gctgccaaaa tcacgggcga gggaaagaaa
    12781 gctttgtacg atctggctct gtcttggaaa aacaaatagt gccggacgag ccggcaccat
    12841 agtgtgaacg gaatcagcgg acacgacccg tcatgcggca cagaacatct ctaccctgaa
    12901 cacggtgggc gacgaccatg gcgacgtgtc cggcgacggc ggcaaacagc agcaagccca
    12961 aattgccgtg gaacgtgttg cccaagtttg ccatccactc gattttttcc ggcgaaccct
    13021 tgcatcactt caacgccgaa cactttcaac gggccgcggc cgctgccgta ttggcggatc
    13081 atgccgataa cgggaacagc aagcatgagc agatacagaa tgccgtggcc tgccgccgca
    13141 gccttgcagt cgctttgcgg acgcttggcg cggttggcaa ccgcccacac gatgcgcaat
    13201 gtaatcaccg tcagcgtaag gaaaccgaaa gatttgtgca ggccgaacag gctgcccacc
    13261 cattccgcct cttcgtatag tggattaaca aaaaccagta cggcgttgcc tcgccttgcc
    13321 gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
    13381 aatcgtccac aggacaatgg tggtcagaat gccgatgaag ccggcaacgg tcagccagtg
    13441 cagcgcgcgt gtcaacgggt cgtaaacggc aggttggttt gtgttcataa aaaacttatt
    13501 atatgtaacg gtttgaacgg acattccgtc gggtcggaat gtcaaaaagg cgcgattgta
    13561 ccaaagaagt tggacataat tttgtttgca ggctgaagat ttgcttaaaa attcattaag
    13621 atgggcggaa caaatagttt gggtacggcg tgttgaaata ccgcttatcc cttataatag
    13681 cgtccgaaat tctgttcgga cggcatcaaa acacacggta atccgtctga accccccatt
    13741 tgaaaccaac aaacaagagc attgaatgaa attcatcgac gaagcaaaaa tcgaagtcgc
    13801 cgcaggcaaa ggcggtaatg gcgcaaccag tttccgccgc gaaaaattcg taccgcgcgg
    13861 cggcccggac ggcggcgacg gcggcaaagg cggtagcgtc tgggcggaag ccgacgaaaa
    13921 caccaacacc ctcgtcgaat accgcttcgt taaacgctac caagccaaaa acggcgaaaa
    13981 aggccacggt tccgaccgtt acggcgcagg tgcggacgac atcgtcctca aaatgcccgt
    14041 cggtaccctc atccgcgacc tcgacaccga cgaaatcgtt gccgacctca cttaccacgg
    14101 acagcgcgtc tgcctagcca aaggcggcaa aggcggcttg ggcaacatcc acttcaaatc
    14161 ctccgtcaac cgcgccccaa aacaatccac gcccggcgaa gaaggcgaaa cccgttccct
    14221 gcaactcgaa ctcaaagtcc tcgccgatgt cggcctgtta ggcatgccca acgccggcaa
    14281 atccaccctg attaccgccg tctccgccgc atgccccaaa atcgccaact acccctttac
    14341 caccctgcat ccaaacttag gcgtggtacg catcgacgaa aaccacagct tcgtgatggc
    14401 cgacatcccc ggcctgattg aaggcgcggc agaaggcgca ggcctcggcc atcgtttcct
    14461 caaacactta tcacgtaccg gcctgctgct gcacgtcgtc gatttggccc cgtttgacga
    14521 aaccgtcaac cccgccgaag aagccctcgc catcatcaac gaattgcgca aatacgacga
    14581 agaactctac ggcaaaccac gctggctggt gctgaacaaa ctcgacatgc tcgatgaaga
    14641 agaagcccgg gcgcgaacag ccgccttcct cgaagccgtc ggctgggact acccgaaacc
    14701 agacgaccgc ttccaattcg acatggaaac cccgcgcctc ttccaaatca gcgcgctgac
    14761 ccaccaaggc acgcaggaat tggtacacca aatcaaccaa tacctgatcg agaaaaaacg
    14821 tatcgaagct gaaaaagcgg aggcggagcg ggcagcgaca aatgttgaga ttgccgagca
    14881 acagcctaaa acggatacgg gcgtgtttaa gccggagtga aaaaagaaag ggtttcagac
    14941 gatatttgtt ctacaaaagg ttgtttgaaa caattttaaa gcaagattga aatggattaa
    15001 aaatgtcatc taaccaatat atccagtcgc ttgagttgac aaactttacc gttcttccaa
    15061 acgatacttt tgaattttct gaaaatctca atgttattgt tgccgaaaac ggttgtggca
    15121 aaacacattt gttaaaaatt ttatacagcc tgctagaagt aacatctaac accaaaaaca
    15181 gattactgaa aacagaattg caaaaaagtt ttgccgataa attactgaat gttttccgtc
    15241 ccgattcctt gggcaggttg agcaaacgtt tgcaggggcg cgggcgtacg gaaatcgttt
    15301 taaaactgca aaacggcact acgcatagta gtctgaattt ttccagtaac tcatcatcgc
    15361 aagtcaatgt tcaaagcatc ggattgaaag aaaatgaata tactccgaca cctatcttct
    15421 tacccagccg cgaattaatt acactttgcc cttggttcac ttctttgtat caaaaccaaa
    15481 gcatcccttt cgaagaaact tggtttgata cctgtatgca attaaatcac ccgcttgcca
    15541 aaggccccag agaaacaaaa attcgagaat tgctcgaacc tattgagaac gcgatgggcg
    15601 gaaaagtatc tgaagaacaa ggaaggtttt atctatcgct gacaaatacg ggcggtaaga
    15661 ttgaagcacc tttggtagca gagggtttgc ggaaatttgt catgattgcc cggttaatcg
    15721 caacaggggc attgctggat aaaggctatc tattttggga tgaaccggaa gctaatctga
    15781 atcctaaatt gatcaaagtg gctgcccgta ttatttggtc tttatctcaa cagggtattc
    15841 aggtttttat tgcaacacac tctctattct tattgagaga attggaactt ttgaagcttg
    15901 aaaataacac ggatttacct gtacgttttt tctcgttgat tgcttccgat gatggaacaa
    15961 aagtggagca agggaacagt attaatgatt tgaacacatt gatattgtta gatgaaaaca
    16021 ttatgcaggc agacaggtat ctgaatattt gaggttaagc tatgactaca atcacagaaa
    16081 agagattaac ctttgctttt cctgaagatt attacgttac caagtatgat gaatgggaac
    16141 attataaaat ttttcagaac agttgtaatt tacgtaataa aattgataca aatgaaaagg
    16201 gaaagaacgg gataaatcaa agtgtagatg acgataatgg cagctcaggt gtagatataa
    16261 tagcccttca tgaatcgaca ctttggctaa tcgaaattaa agattattac cgacttgggc
    16321 ttgagccaaa tgcccaatca atagatgaaa aactttctga cctaccatat ttaatcgcaa
    16381 gaaaaattcg agattcattg gcaggattgg tttctgctaa atttaaagca gaaaaacagg
    16441 aagaaaaaga tttttctcgc ttggctttag attgtaacga aataaaaata gtccttcata
    16501 ttgaaatgcc tagtataaga agtaaacttt atccctcgtc atcagattta gcaaacttat
    16561 taaaagataa gtttaaatta tcagaattta caaaaaattt tgcaaactgt tatgcagaac
    16621 caattttcac aaatatcagc catattaata atccgcaact acgtaatgtt ccttggtccg
    16681 tatctacagg aacagaacag aaattatcat ccgagcaaca aagatcaatc catagtccca
    16741 tgaccaccat ctacaacacc cttacccgcc aaaaagaacc ctttgccccc atcgaccctg
    16801 aaaacgtgcg tatgtacgtt tgcggcatga ctgtttacga ctattgccac ttaggccatg
    16861 cccgtgtgat ggttgtgttc gacatgattg cccgttggtt gcgcgagtgc ggctatccgc
    16921 tcacttatgt acgcaacatc accgacatcg acgataaaat cattgcccgc gcggctgaaa
    16981 acggcgagac catcggcgag ctgactgcgc gtttcattca ggctatgcac gaagatgccg
    17041 atgctttggg tgtgttgcgt cccgatatcg agccgaaggc gacggaaaac atcccacaaa
    17101 tgattgccat gattgaaacc ctgattcaaa acggcaaggc atatcctgcc gcaaatggcg
    17161 acgtttacta cgccgtgcgc gagtttgccg cttacggcca attgtcgggc aaatcgttgg
    17221 acgacctgcg cgcgggcgaa cgtgtggaag tggacggttt caaacgcgat ccgcttgatt
    17281 ttgtattgtg gaaagcggcg aaagcaggcg agccggcatg ggaaagcccg tggggcaacg
    17341 gccgtccggg ttggcacatt gaatgctctg ccatgagcga aaacctgttc ggcgatacct
    17401 tcgacatcca cggcggcggc gcggatttgc agttccctca tcatgaaaat gaaatcgccc
    17461 aaagcgtcgg cgcgacgggg catacctgcg gtcatcatca cgcgcaaacc caccacgggc
    17521 aaagcatcgc cagccacgtc aaatattggc tgcacaacgg ctttatccgc gtggacggcg
    17581 aaaaaatgtc caaatcactg ggcaacttct tcaccatccg cgaagtgttg aaacaatacg
    17641 accccgaagt cgtgcgcttc ttcatcctgc gcgcccacta ccgcagcccg ttgaactatt
    17701 ccgacgcgca tttggacgat gcaaaaggtg cgttgacccg tctatacacc accttgaaaa
    17761 acacgcctgc cgccgcgttt gatttgtctg aaaacgccaa cgactacacc cgccgcttct
    17821 acgccgccat gaatgacgat ttcggcacgg ttgaagccgt tgccgtgctg ttcgaactgg
    17881 caggcgaagt gaacaaaacc aatgatgcgc acctcgccgg ctgcctgaaa gccttgggtg
    17941 gcatcatcgg actgctgcaa cgcgatccga ccgagttcct gcaaggcggc gcggcttcag
    18001 acggcctctc caacgaagaa atcgaagact taatcgcacg gcgcaaacaa gcgcgcgcgg
    18061 ataaaaactg ggcagagtcc gaccgcatcc gcgaccttct gaacgaacac aaaatcattc
    18121 tggaagacaa cgccggcggt acgacttggc ggcgcggtta aaatccatcg aaaagggtaa
    18181 atgtgccgca ttttgccctt ttttgttcag acggcatttt tattgtgaga acttattttt
    18241 attatgctta aaatcatttc cgccaacgtc aacggcatcc gctccgccta caaaaaaggt
    18301 ttttacgaat acatcgccgc atcgggcgcg gacattgtct gcgtgcagga actcaaagcg
    18361 caggaggctg atttgtctgc cgatatgaaa aatccgcatg ggatgcacgg ctattggcat
    18421 tgcgccgaga agcgcggtta cagcggcgtg gcggtgtaca gcaaacgcaa acccgacaat
    18481 gtgcaaatcg gtatgggcat tgaagaattc gacagggaag ggcggtttgt gcgttgcgat
    18541 ttcgacaggt tgagcgttat ttcgctttac ttgcccagcg gcagcagcgc ggaagaacgc
    18601 cagcaggtga aataccgttt ccttgatgcg ttttacccta tgctcgaagc gatgaaaaac
    18661 gaaggacgcg acattgtcgt ttgcggcgac tggaacatcg cccaccaaaa catcgacctg
    18721 aaaaactgga aaggcaatca gaaaaattcg ggcttcctac cggaagaacg cgagtggata
    18781 ggcaaggtta tccacaagct cggttggacg gatatgtggc gcacgcttta tcccgatgtg
    18841 ccgggctaca cgtggtggag caaccgcggg caggcgtatg cgaaagatgt cgggtggcgc
    18901 atcgattatc agatggttac gcccgaactt gccgccaaag ccgtgtccgc acacgtttat
    18961 aaagatgaaa aattttccga ccatgcgccg ctggtcatgg agtatgacta tgctgccgaa
    19021 taaagttttg ggtaaatatg attggaatgt ggacggaaaa acaggaatag gcgcagcatg
    19081 ggttgtcgct gcgtttatcc tgcccatatt ggtgtgggcg atatttatgt tgtcgcggat
    19141 gcaaggctgg cttgcgccga ccaaggcaaa cccgatatgg gcgttggtgt ggctgctgat
    19201 ctgcatgccc tgcctgctga ttgcgtccaa atgtttgggt tggaaaggct ggcggctgtt
    19261 ggcaaacgtt cttgcctgcc tcgccgtgtg cgctctgttg agtgtgcctg ccgcgctgct
    19321 gattgcgttc acgctgcaag acctgctcaa ataggcgcgg acaatggaaa gtttcgagct
    19381 tgaggatttg acgctgtggc tggtacgcga tgcggatgaa gcggaaatgt ggatagaccg
    19441 ctgggcgatc agttatcccg tcgtgcagat gtccgaagcg tcggccggtc aaagcatagg
    19501 ggaatggcag gcagggcttc agacggcatt tgaacgcata cgcggcaaat acgtcgccgt
    19561 tgtcgcacac ggtgcgggcg cggccgcatt tttggcgtgg ctgtatcagg cagacatcct
    19621 gacacggaag aaaattgcca acatcatcct tgtaccgcag cgtcccgata tttttcccga
    19681 cgatgcggaa cacactttcc aacgcgtccg ctgtccctgc cgtgccgcat tggttgtacc
    19741 cgaacacggc ggcgtgccgc acggttgggc gcaaaaacag gcggatttgt ggaacgcccg
    19801 cctgttggtt tccccgcatt cgggcagttt gaacggtatg ctcggcggct ggcagtgggg
    19861 tatgaagctg atgcaggaaa tgttgctggc gtgaacgctt gccttatcgg aatttcggct
    19921 gtagcaggaa gtccgcatat cgggctgttt tcattgacgt gcaggcgcat caggttcgga
    19981 taaagtcggg ctgccgcata ccgccggaaa tggaatcctt tattttttac gtcggcagtg
    20041 ccggcagggc gttatccggc agggttgcaa ggaaaatgcc gtctgaaaca ggcttcagac
    20101 ggcatttcca ttcaaacagg gctcacaggc tgcccaacac gatacgcaat acgcggcgca
    20161 gcggttcggc cgcgccccac aagagctggt cgccgacggt gaacgcgctg atgtattcgc
    20221 cgcccatgcc cagtttgcgg atgcgtccga cagggacgga cagcgtgccg gtaactttgg
    20281 caggagtcag ctcgcggatg ctggcttctt tttcattggg gatgactttc acccagtcat
    20341 tcgcgcctgc caaaatcgtt tcgatttcgg aaacaggcag gtcttttttc aacttcagag
    20401 tgatggcttg gctgtggcag cgcatcgcgc cgacgcggac gcacaggccg tcaatcacgg
    20461 ttggattgtc gctgcggccg aggattttgt tggtttccgc gccgcctttc cattcttctt
    20521 tggactggcc gttgcccaaa tccacgtcaa tccacggaat caggctgccg gcgagcggta
    20581 cgccgaagtt ggctttcgga tagtcttcgc tgcgcaggaa atcggatact ttgcggtcga
    20641 tgtcgagaat cgagccggca ggatcggcaa gttcgtccgc cacttgggcg tgaaccgcgc
    20701 ccataccgct gatgagttcg cgcatgtttt tcgcacctgc gccggaagcg gcttggtagg
    20761 tcatgctggt tgcccattcg accaaatcgt tttggaacag gccgcccaaa gccatcagca
    20821 tcagggagac ggtgcagttg ccgccgatgt agtttttcac gccgtttttg aggccgttgt
    20881 cgataacgtt gcggttgaca gggtcgagga cgataatcgc gtcgtctttc atgcgcagag
    20941 aagatgccgc gtcaacccag tagccgttcc agccgctgtc gcgcagggct tggaacacgg
    21001 atttggtgta atcgccgccc tgacaggtaa cgatgatgtc catttttgcc aattcggcaa
    21061 catcgttggc atccaacaat gttttggctg cctgaccgaa atcaggggct gcgccgccga
    21121 cgttggaagt ggtaaagaaa aacgcttcag gaatgtgggc gaagtcgttt tcttctttca
    21181 tacgctgcat caaaaccgaa ccgaccatac cgcgccagcc gacgaaacct actttcattg
    21241 tgttgctcct tgaggaaaaa atcgtta
//
LOCUS       DAWHRA010000018        20696 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWHRA010000018 DAWHRA010000000
VERSION     DAWHRA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.018"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(64..240)
                     /locus_tag="WIB30_000504"
     CDS             complement(64..240)
                     /locus_tag="WIB30_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796629.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            complement(243..1601)
                     /locus_tag="WIB30_000505"
     CDS             complement(243..1601)
                     /locus_tag="WIB30_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ3796630.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGNECAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1726..3372)
                     /locus_tag="WIB30_000506"
     CDS             complement(1726..3372)
                     /locus_tag="WIB30_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ3796631.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3395..4873)
                     /locus_tag="WIB30_000507"
     CDS             complement(3395..4873)
                     /locus_tag="WIB30_000507"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ3796632.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVAGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4898..6643)
                     /locus_tag="WIB30_000508"
     CDS             complement(4898..6643)
                     /locus_tag="WIB30_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ3796633.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6704..6967)
                     /gene="ftsL"
                     /locus_tag="WIB30_000509"
     CDS             complement(6704..6967)
                     /gene="ftsL"
                     /locus_tag="WIB30_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ3796634.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(6964..7950)
                     /gene="rsmH"
                     /locus_tag="WIB30_000510"
     CDS             complement(6964..7950)
                     /gene="rsmH"
                     /locus_tag="WIB30_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ3796635.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRRIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7947..8402)
                     /gene="mraZ"
                     /locus_tag="WIB30_000511"
     CDS             complement(7947..8402)
                     /gene="mraZ"
                     /locus_tag="WIB30_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ3796636.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVSAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8681..9898
                     /locus_tag="WIB30_000512"
     CDS             8681..9898
                     /locus_tag="WIB30_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3796637.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHIIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTDSAAYICEAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WVGFRFGDICHKL"
     gene            10030..10106
                     /locus_tag="WIB30_000513"
     tRNA            10030..10106
                     /locus_tag="WIB30_000513"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10064..10066,aa:Met,seq:cat)
     gene            10114..10189
                     /locus_tag="WIB30_000514"
     tRNA            10114..10189
                     /locus_tag="WIB30_000514"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10147..10149,aa:Ala,seq:ggc)
     gene            complement(10480..11301)
                     /locus_tag="WIB30_000515"
     CDS             complement(10480..11301)
                     /locus_tag="WIB30_000515"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ3796638.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEHLFNPLSVAVMLVLGGLFILWVEKRQSWAEPKIADVDALRPIDALMIGVAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11334..11978)
                     /locus_tag="WIB30_000516"
     CDS             complement(11334..11978)
                     /locus_tag="WIB30_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ3796639.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11981..12826)
                     /gene="ftsN"
                     /locus_tag="WIB30_000517"
     CDS             complement(11981..12826)
                     /gene="ftsN"
                     /locus_tag="WIB30_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ3796640.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALYEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREEPDGQAVRKKVLTEEREQTVGEKAQKKDAETVKKQAVKPSKETEKKASKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(12932..14428)
                     /locus_tag="WIB30_000518"
     CDS             complement(12932..14428)
                     /locus_tag="WIB30_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ3796641.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLE
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14442..14762)
                     /locus_tag="WIB30_000519"
     CDS             complement(14442..14762)
                     /locus_tag="WIB30_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ3796642.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15245..16771
                     /gene="putP"
                     /locus_tag="WIB30_000520"
     CDS             15245..16771
                     /gene="putP"
                     /locus_tag="WIB30_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ3796643.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSTAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRATR
                     "
     gene            complement(16861..17028)
                     /locus_tag="WIB30_000521"
     CDS             complement(16861..17028)
                     /locus_tag="WIB30_000521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796644.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17020..20625
                     /gene="putA"
                     /locus_tag="WIB30_000522"
     CDS             17020..20625
                     /gene="putA"
                     /locus_tag="WIB30_000522"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ3796645.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRASRTKASGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYAGEAENTLPKDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVAHLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4862 a         5988 c         5027 g         4819 t
ORIGIN      
        1 tcgggcctgc gcccgtttga ggttggcttg ccgcccggca aggtttcgga cgacttttgc
       61 agattaacgt gcagagactt tcccaatcac gcaaatcggg taactccccg gatttttgcc
      121 gccacccaag tctaaacata aatcttcata aagatacgcc tgcgtctgca ttccggcata
      181 aaacgcgccg accagcgcgg caatcaacaa taaggcttta aaaaaacgtc gacttttcat
      241 tttcacttat cctccaatgc ctcgaccact tcttccatct gcataaagcg cgaacctttc
      301 accaacacgg tggcgcgttc gggcaaatcg tggcgcaaca cttgaatcaa cgggtctttg
      361 gcggcgaacc acaaaccgtc cgcgccaaat ttttccgccg cttcgacgct gttgtcgccg
      421 acaaaataag ccgcttcgat gccttggtcg cgggcgtacg cgccgacttc ggcgtgcatg
      481 gcggcggctt cgtcctcacc cagttcgccc atatcgccca tcacgaaaat acgcggcgca
      541 ggcatacgcg ccaacacgtc aaccgcagct ttcatgctgt cagggttcgc attataagta
      601 tcgtcaatca gggttgcgcc cttgattccg gatttgacgt tcagacggcc tttgatattg
      661 ctgaagcctt tcaaaccttc cgccacatcg ttcaaactca aacctgcagc caaagacagc
      721 gcggcggcgg cggcggcgtt gtggacattg tggcggccgg gaacgggcag aaccacggct
      781 gcgcactcgt tgccgcacac caaatcaaat tcgcacgaca acggtttcag cacgatattt
      841 tccgcgtgga catcgccgct atcgatgccg aaagtgcgcg tattcaaatt aagcgttgcc
      901 gttttgaaga cagccatatt ggcatcttct tgaggaatca gcgccatgcc gtctgaacat
      961 aagccttgat aaatctcgct tttcgctttg gcaatatcgc ccactccgtc gaaaccgcag
     1021 ccgacatggg cgcgcatggc gttgttgacc aatgcggcat cgggtttggc gatttgtgtc
     1081 aaaaccgcca gttcgccaaa atggttcata cccatttcaa tcacggcata gcggtgtttt
     1141 tcgtttaatt tcaacaaagt caacggcaat ccgatgtggt tgttgaagtt gcctgccgtc
     1201 gccaaaacgg cattatcgcc gaaacggcgg cgcaataccg cagccaacat ttccttcacc
     1261 gtcgtcttgc cgcccgagcc ggtaataccg aacacaaacg ggttcacatt ctcgcgccac
     1321 gccttcgcca acatttgcaa cgcggtaagc gtgtcatcga ctttcaacgc gccatccatt
     1381 gcaacgcaat cttcgcgcga aaccacaacc gccgccgcac ccgcagccaa tacgtcttca
     1441 acaaaatcat gcgcatcaaa ccgcccgccc gccaatgcga aaaacacatc gcccgcgcgg
     1501 atgtcgcggc tgtcggttac gatgcgcgac acgggtttgc tttcagacgg catcggaagc
     1561 ttgagggctt ggcagatgaa atttaggtcc agtggtttca tatttacttt cgttaatatt
     1621 cgggcggcgg acacatcggt agcggctgat ttttttatcg cctgttttgc tgtggtaaaa
     1681 cacagattat tttcccattc tcattcggca ttttttctgt acgtatcatt ttttagacgt
     1741 atttttagtc gatttgcctt ttcccgcata ccacggcgcg gggtcgtcgg gcagtccgtc
     1801 gataaaggca aggttattgc cttcgccctg cacatcggga acattccccc aaaaatcata
     1861 gccgtcatcg ggcaactcgt cggtttcgat acccgtccaa ctgccgaatc cgcgtaaaaa
     1921 attaaacgcg ctgcgcctca ctttaatcat ttcgcgccgc gtatcatcgg acgaaatttt
     1981 aaccagcggc acgccgtagc tttgacgctt ccacgcacca tgtgtcaacg ttcgctcgcc
     2041 gcctttaccg acatgcatca aaccgtggtc ggaaaagtaa accaaagacc agctttcttt
     2101 attttcattc aatatcttaa ctgtatcttc taaaaattta tcggtttgcg cgatggtgga
     2161 aacatagcag gatatttttt cagtttgata ctgaaaccgc cgcgcatcct tatccaaacg
     2221 tgtgcaaaaa tcactgtgcg aacccatcag gtgcatcaca atcagccgag gcttcgtgcc
     2281 tgcatttcct atcaaaaccc gtttgaacgc cggcaacaaa aggcggtcgc tcaaccccgc
     2341 gcttttgcca taatcacccc tttgggtaaa ccacggataa tcgctgcgta gggcataggt
     2401 ggaaatttcg ttggcaaaat gccccaacat tccttgatta gacagccacg ccgtccgaaa
     2461 acccgcctgc ttcgccaagc tgacgatgtt attgttcggt tctcccggca gccccaaagt
     2521 ctgcggcagc gaaagattcg tcgcgtgggc ggtcgattgg taaccgttta tcaacagccc
     2581 tttggtctga ctcaaaaaag gcgtatcggg caatgggaaa ccgtaaacat tcatataatc
     2641 cgaacgcgcg ctttcaccga taaccacaac ataattttta tacttgggcg caacatgacg
     2701 gatatgccat gttgacgctt tttttgctgc ttccaaaatg tgggcgcgct tggcggcata
     2761 ttgtgccggc gcggaagcca aatcgtaata caggtcgaaa atattcaaca acaggccggc
     2821 atcaggttct cgcaaatctt tatcgctggc gattttatcc atcaccgcgc aggacaaaat
     2881 caaagtcaat aatatagtca gccatatttt gctgcggcgt ttatagtttt ttacgtcagc
     2941 aaataccccc cccgatacac aatatctcca aactgtcaag ccaaaaatga aaaataatgc
     3001 ctgcacaaaa taaagcgacc cgggaagatt gccgacaaat tcacgcgcct cggcaggatt
     3061 gctttccaat atcgaaccga ctatctgata agacggcgca ccatacagcc agccgaccgg
     3121 caaatatagg gcggttgtgc cgacgtaaat cagtaaaaca actgaagaaa cgcgcgggaa
     3181 accgcgtgcc aataataata aaaatacaac cgaagccaaa gcacccacgg cagaataacg
     3241 gtagccgtat tcatattcca agtgataacc tgatgcgatg gccgcgccaa aacaaaaggc
     3301 taaaagcgcg caaatgccca atgtattttt taatgtctga tccatatttt ttattcgggc
     3361 ggattgtttc atacgatgcc gtccgaatat cccattattt acgagttaac aaagcctgtc
     3421 cgacgatttc aagatcggaa aaacggtgct tcacgccttg tacatcctga tagttttcat
     3481 gccctttgcc ggcaatcagg atgatgtcgt ttgcggcggc ttgttcaacc gcataacgga
     3541 tggcggcggc acggtcggct tcgacgcatt cgggcgcggg aacggcaggc aggatgtcgt
     3601 tgatgatgtc gtgcggattt tccaaacgcg gattgtcgct ggtgacgacg actttatccg
     3661 cgccctgtac ggctgccgcg cccatcagcg ggcgtttgcc gcgatcgcgg ttaccgccgc
     3721 aaccgaatac gcaccacaaa gccgcaccct gcggtttgat ttcctgcaag gtggcgagtg
     3781 ctttttccaa tgcgtcgggc gtgtgggcat aatcgacaac gaccaaaggt ttgccgctgt
     3841 tcatgatgca gtccatgcgc cctgaagcgg gacggatttt tgccagcaca tccaatacat
     3901 tatcaagcgg atagccgttg gcgcacagca aggcgatgca ggcggcgagg ttttgcgcgt
     3961 tgaaccgtcc gagcaggcgc gtgcggcatt tcccttcgcc ccacggggtt tggaatacgg
     4021 ctgctatgcc gtctgaagag gcggtaaagt cggtaatgcg gatgtcggcg tgttcgctga
     4081 aaccatagct gtaaacggcc aaatcgggac agtctttttt cagacgacct acgagttccg
     4141 cgccgtattc gtcatccacg ttgatgactg cgtgtttcaa gccgtgccag taaaacaggc
     4201 gcgacttgat ggcaccgtag gcttccatcg tgccgtggta gtcgaggtgg tcgcgggtga
     4261 ggttggtaaa gattgcgctg cggaatgaca cgccgttgac gcgcgactgg tcaagcccgt
     4321 ggctggagac ttccatcgcg gcgactgttg cgccttgttg acggaaacgg tagagcaggg
     4381 tttggacatc gacgggggcg ggtgtggtat gcgtggtttc ttccaatgca ccccaaaagc
     4441 cgttgccgac cgtgccgaca atggcggttt tttcgcccaa caaatcggca gcttgcgcca
     4501 gccattgtgt gatggaggtt ttgccgttgg ttccggctac gccccaaact ttgaggccgt
     4561 ctgaaacgtt gccgtaaact tgcgccgcca atatgccggc acggtgtttc aaatctttga
     4621 tgccttgatt ggggactttc cattcgggat tccacgcaaa tttgccgtcg tcgtcccaaa
     4681 aaacaaaagc cgcgccgttg gcaacggcgg cggggatata actgcgtccg tcggcatatt
     4741 cgcccggaca ggcaacgaaa atatcgcctt gtttgatttg gcggctgtct gaatgcaaca
     4801 aacgccctgc cgcgtttgca cacgacagag tcgggatgcc ggtttcagcc aaaggggtta
     4861 acttgctgaa cataaaacaa tctcgttgat actcggatta agacggtgtt ttgacggctg
     4921 cggcggtcag tggcttggtc ggggaaatgc ccaagatgtt caggctgccg cccataattt
     4981 ttttgaaggg cggccctgcc actacgccgc cgtaataacc gtgggcagtc ggttcgtcaa
     5041 tggttaccgc cacaatcaca cggggatttt tggcgggggc aaaaccgata aaggtagcga
     5101 tgtgtttgtt gtcggcataa cgcccgttga cgaacttgcg cgccgtgccg gttttcgcgc
     5161 cgacatcgaa accgtccacc gcacccgccg taccggtgcc gcccggctcg gttacggaaa
     5221 ccatcagatt gcgtacctcg cgcgcggtcg attctttgaa tatgcgtttg ccttgcggcg
     5281 caaccgcctg tttttcaaag ctgaccggca gtaaaacgcc gtcgtgcgtc agtgcggtat
     5341 aggcgcgcgc caattgcagc aggctcaatt gcaggccgta accgaaagac atcgtcgcct
     5401 gttcgatagg ccgccacctg cgccaatttc tcaacaaacc tgcagtttcg cccggaaagc
     5461 ccgagtgcat acgcacaccg atgcccaact catgatagaa gtcatacatt tcttcggcac
     5521 cgaaacgcgc agacagtttg cttgtgccga cgttggacga tttctgcatg atgccgcgca
     5581 catccaaaga ggggtaaaca tgggtatcgc gcacgggaga cggtccgatt ttataaggct
     5641 gcgtattcag ccgttcgttc aaatcggttt tgcccgcatc caatgccttc gcaatcacaa
     5701 acggtttgat tgccgaaccg ggttcgatca tatcggttac ggcacggttg cgccgctgtt
     5761 cgctgtctgc ccggccgggc ctgttgggat cgtaggcggg cgtattggcc aaggcgagga
     5821 tttcccccgt gcgggcatcc aaaaccacca ccgttccggc ttttgcctga tggtattcga
     5881 ccgccttgtt caactcttca taggccaagg tctgaatcct ctgatcgagg gaaaggatga
     5941 tgtctttgcc gtttttcggg gctttattgc gcggggagtc caagctgtcc acaatattgc
     6001 cctgccggtc ccgcaaaacg acttccgcgc cgtcttcgcc atgcaggctg tcttcaagcg
     6061 aaagttccaa accttcctga cctttgccgt caatatcggt aaatccgatg acgtgtgcaa
     6121 acaggttgcc catcgggtaa tggcgtttta attctttttc aaatacaaag ttttccaaac
     6181 ccaaggcttt gacctcttcg gcaaccttgg gatcgagctg ccgcttaatc cagataaacg
     6241 acttgccttt ctgttcgagc ttgttcctca aaacatcaac cggcacatcg acaagctcgg
     6301 acaggcgttc caattgtgcg gcagacggca tttccttcat ctctttaggc acggcaaaca
     6361 gggactccgt cggcgcactc aacgccaaaa ccgcaccgtt ccggtccgaa accgtaccgc
     6421 gtgtagccgg caatgtttga gtccgcacaa tccggttgtc gccctgttct ttcaaaaagt
     6481 tatacgttac cgtctgcaga tacagtccgc gagcaatcag accggcaaac aagaccgcta
     6541 ttgccatcag gacgaagctg atccgtccgt tactggtcat cggctttttg acctgctctt
     6601 ctttgggcag catccgaggc ttatattcgc tcttaatcaa catttttact tctcgttatt
     6661 attatcctga cgcaggaatc cgattccggc acacaggctg cttctatctt tgatgctcca
     6721 ccataaaggt attgcccgaa accggcggat ggaggttttg tttttctgcc gccgccctga
     6781 tcgcttcgtg gttcgccaaa cgcgcctgtt gcagcctcat ttgcgcataa tcctgctcca
     6841 aggcgatttc ctgttttttc gccttatcca aagctgtgaa attgagcctg tactggtttt
     6901 gctgcatcac aacggaaaaa gcggaaacgc acaccgcaag cagcagaagg aaattcaatt
     6961 tgttcattgc cattcagacg tttttctctg tgattgttcc ggtatcggac cggcagtccg
     7021 ctccgccaca cgcaaaaccg cacttctcgc cctcggattg gcggcaattt ccgcctcacc
     7081 cggctttaat gccctgccca cgattttcag gggcagctcg ggcaaatccg cttccctgac
     7141 cgccgcccag cgcggcaggg gcgcgtgttg cgaatatttt ttgacaaact gcttcacaat
     7201 gcgatcttcc aacgaatgga aagcaatgac cgccaaacgt ccgccctctt tcagacgaca
     7261 catgacctgc ggcaatactg cccctacttc ttcaagctcg cggttaataa agatgcggac
     7321 cgcctggaag gtgcgcgtcg caggatcctg cccccgctcg cgagtacgga cgttttgtgc
     7381 cacgatctgc gccagcttgc gggttgtatc gattggactt tccgcccgtt gcgcaacaat
     7441 ggcgcgcgca atccggcggc taaaccgctc ttcaccataa ttcttgatta cctcgtgcaa
     7501 atcctgttcc gacgcaaccg ctatccactc tgcggcagac ataccgcgcg tcgtatccat
     7561 acgcatatcc aaaggggcat cgaaacggaa gctgaaaccg cggctgccgt catcgatttg
     7621 cggggacgaa atccccaaat caaacagcgc accgtccacc ttgccgatac ccaaaccgtc
     7681 caatgccgtc tgaaacgaag caaaaccgcc atgcacgaca ccgacccgtt tgtccgaacg
     7741 cgccagctct tctgccacag caatcgcctg cgggtctttg tcgaaaacaa tcaaccgccc
     7801 cgcatcgccc aaacgcgaca aaatcagccg ggaatgccct cccctgccga acgtaccgtc
     7861 cacatagaca ccgtcttcgc gcacggcaag cgcatccacc gcctcattca gcaagaccgt
     7921 gatatgccgg taactttctg ctccactcac aattgcaaat ccgtctgact caactggaag
     7981 gcaagttcgt caggatcgtc atccaaagcc tgaaccatct cagcctccca ctgctcgcga
     8041 ccccaaagct ccaaacggtt ggcacgaccg accaaaacga cttcacggtc gaaatccacc
     8101 ctcttcctca gtccggcaga aaccagcacc cggccggcgc tgtcccattc caaaatttcc
     8161 gcgttatgca gcaaaagatt ttgaaaccgc cgcaaaacag ggttatccgc cacttttaag
     8221 tttaaaagtt gcgccgcaac cttttcccac tccgcaacag ggtacatcaa cagcttgtgt
     8281 ttcgactcga gcgttaccac tacggcaggc gtatagaggc gcgacagaat gtcacggaat
     8341 ttggcaggaa cagccaaccg ccccttactg tcgatgctta attcgtgtgc gccgccgaac
     8401 atgacatgtc ccaagccgaa atcaaaatca caagggtaaa agagacactt tgccccacaa
     8461 ttccccacca atcgacacta taagaaattt taaacactcg gtcaaatcag ggcatgaaaa
     8521 ctcattaaca tatctgaaat ttttattcct tttaaaaaca ataagataaa aaatgacgac
     8581 aacggcacgg cgggtgcggt acagaataat cgaaccaata aacaactata tatgattaat
     8641 ttaataatat aaacacaata tatagtatta agataaagcc atgacagcac ccgtaccaac
     8701 gtgtaatatg tcgggaaatc caataaattt acacaagcta acacttatca tgcccctccc
     8761 cctcccctct cccgaagcac ggcaattctc gctcaaactg caaaccctca ttgccgaaaa
     8821 aatcggcaaa cacggcaact ggattccatt ttcacgtttt atggaattgg ttttatacgc
     8881 tccgcaatac ggctactaca ccggcggcag ccataaaatc ggcaataccg gggattttat
     8941 taccgcaccg accctcacct ctctgtttgc acagacactg gcacgccaac ttcaagaact
     9001 tctatcccaa acggcgggca atatctatga attcggcgcg ggaaccggac agcttgccgc
     9061 cgatttgttg ggcagcattt cggacggcat cagccgttac tatattattg aaatatcgcc
     9121 ggagctggca gcacgtcaga aaaacctgat tcaagcacgc gcaccggaag catctcaaaa
     9181 agttgtccac ttgaccgcac ttcccgaagc gtttgacggc atcatcatcg gcaacgaagt
     9241 actcgatgcc atgcctgtcg aaatcgtccg taaaaatgaa ggcggctcat tcgagcatgt
     9301 cggcgtttgc ctagataatg accgttttac ctattcggca cgaccgctgc acgacttgca
     9361 gctatctgcc ttggcttccc tctattttcc tcaaacagat tatccctata ccagcgaact
     9421 acatccgcaa caatatgcct ttatccgcac ccttgcctca agactcgaac acggctgcat
     9481 gatattcatc gactacggtt ttgatgcagc gcagtattac caccctcaac gcaatcaagg
     9541 tactctgatc ggacactacc gacatcacat tatccacaat ccttttgact tcatcggatt
     9601 ggccgacctg accgcacatg tcaactttac cgacattgca caagcaggga cggatgccgg
     9661 attagatttg ataggttacc ttccccaatc ccatttctta ttgaacttgg gcattaccga
     9721 gctattggca cagacgggga aaacggattc ggcagcctac atctgtgaag ctgctgccgt
     9781 tcagaaactg attgaccagc atgaaatggg cgaactgttt aaagtcatcg cattcggaaa
     9841 aaatatcggc atcgactggg taggattccg cttcggcgac atctgccaca aactctaacc
     9901 ctcatgccgc ctgaatccgc ttcagacggc ataaactttt taacatttaa aaacagtcaa
     9961 ctaattcaaa attaaaaaat acggcttgtc aaaaaaacag aaaaacatat aatagcgtct
    10021 tcacgaaacg gcgaattagc tcagtcggtt agagcagagg aatcataatc cttgtgtccg
    10081 gggttcgagt ccctgattcg ccaccaaatt ttcgggggta tagctcagtt ggtagagcgc
    10141 ttgcatggca tgcaagaggt cagcggttcg atcccgctta cctccaccag ataaaaaagc
    10201 acagaccgta aaaaggtatg tgctttttta ttacctgatt accggcaaat aaagaataaa
    10261 ccactgcctt caaaacaggc aatcgacttt aaacctatcg ccccgcctgt cctgatttta
    10321 tagtgaatta aatttaaacc ggtacagcgt tggctcgcct tgccgtacta tctgtactgt
    10381 ctgcggcttc gtcgccttgt cctgattttt gttaattcac tatatcagcc cgccagacaa
    10441 acccgacccg aataatgtct tcaggtcggg tttatggttt cattcccaac ttatccagcc
    10501 tgacagccac aatataatga tggcaatacc aaaaacaatg cggtaatagg caaaaggaat
    10561 ataatttttc ttggaaacaa acctcagcaa cgctttgacc gccaccaagc ctgaaacaaa
    10621 ggcagcaaca aagccaatca aaatcaaacc gacatcatgc agggtgaaaa accggtaatg
    10681 cttcaggaca tcataagccg ttgcggcaac catcatcggc acagccaaga aaaacgagaa
    10741 ttctgtcgca gttttccgtt cgattcccca aagcatcccg cccataatcg tactgcccga
    10801 acgggacgta cccggaatca gtgcaaacac ttgggcaacg ccgatcatca aggcatcaat
    10861 cggacgcaat gcatcaacat cggcaatttt aggctctgcc cagctttggc gtttctccac
    10921 ccacaaaata aacaaaccgc ccaaaaccag catgaccgcc acactcaaag gattgaacag
    10981 atgttcttta atctgtttgc cgaacaacag ccccatcacg gcggcaggta taaaagcaat
    11041 ggcaagatta aggacgaaac ggttggcttt ccggtctttt cccacgccat gcaacacatt
    11101 gctgaaacgt tgccggtatt caaacactac cgccaaaact gcaccgagct ggatggcaat
    11161 ttcaaaaacc ttgtgattgc tgtgaaaacc aatcagattg ccgaacacaa tcaaatgccc
    11221 ggtgctggaa atcggtaaaa attcggtaaa accttctacc aagcccatca tcagggcttt
    11281 caggacaatc agaaaatcca ttgcttgcgc ttctttcgga tacgggagtt cggctatttc
    11341 tgtacagcag gggtctgacg cttgcgttct tccctgactt tggcaaccaa ttctttaatc
    11401 gtatgaacgc cgccgtcaaa gccgttattg aagataacgc ggtattttcc gccgacaata
    11461 acggtcggcg tgctgtcaat accgtattgt tccgtcagtt tctgcatttt taatgcggcg
    11521 gcggcagctt cgggagaatc ataggcgcgc atcagttttt tgccgtcaaa gcctttttga
    11581 gacaaagccc actttccggc aaccgacctg ttttccaagc ggattttttg ttcgtaaact
    11641 gctttaaaca cagcagggtt tgcctgatat ttcaaacccg acaaattgac ggcggccgcc
    11701 atcctggcca gaccgagcat ttcaggctgc cagaccacgt gctccgtcct caaataggca
    11761 tcagacggca atgccttgcc cagtttcaat aacaaaggat cgaaatgatg gcaatgtacg
    11821 cagaaatagc cgaaaaattc caaaacctca attttacccg actgttcttg aggaatgggt
    11881 ttatccaaca caagatagtc ttccccttcc gtcagggcat atgcctgcgc ggacaacact
    11941 gccgacagca gcagcggcaa cagatgtttg agcttcataa ttatttgctt tcgatagaac
    12001 ggatcaggct ggcgacttca tgttttttca actcgtcctg catttttttc accgcatcgg
    12061 cagacatatt gccgctttgc acccggtaaa gcgttttatg tcccgcctga taaccgacca
    12121 ccttggaaga tatgcccaag attgccagtt tggcacgctg cccttccgcg ctccggcggt
    12181 cggcatacgc gcccatttgc agataatgcg ttgcttccgc cttgtcggac gttttcattt
    12241 tctgcacttc tttggcggcg gcactgcgcg ctttttcgat gctgccgctg ttgaggattt
    12301 gttccggggt cggtttgggt gcaacttttt ccttttccgc ctttttctct tcttttgaag
    12361 cttttttctc tgtttcttta gacggtttta ccgcttgttt tttaaccgtt tcggcatctt
    12421 tcttctgcgc tttttccccg acggtttgtt cacgctcttc cgtcagcact ttcttacgca
    12481 ctgcctgtcc gtccggctct tcccgttccg gctcgcccgc cttttcttca acctcgtcgg
    12541 ctttatcggc agctttttcc gcatccgact gctctgcctc tgtcgcagca tccggttcgt
    12601 acaaggcgtt ttgatcggcc ggttcaggtt ggatgtcttc cttaggctgg tttttcggtt
    12661 tcaggatttc cgtttctgca ggctgcttcg acgaagccgg gattttgaac gcattttgac
    12721 cgctctggtt cagataaaac aaaataccgg caataatgac cgtcgccagt atcaaaccga
    12781 agaaaaaacc ggacagacct tttccggatt gggaaaattt gttcataaac ataccttaat
    12841 gtgtttcaga cggcattaac gccgtttgct tacgggcgga tattctaaca atatcgccat
    12901 atttgggcaa aacctgcttc cattcccgtt cctataaagc gcgacggaaa cctatggctt
    12961 tcatgacgtg gcttctgccg acttcttcgt cgcccgccaa atccgccaat gtacgcgcca
    13021 cgcgcataat gcggtggaag ctgcgggcgg aaagggagag tttttccagc aggccgccca
    13081 atgcttcctg cgcttctttt tgaatgcggg cggatgtgtc gagttcactg acactcaagg
    13141 cggcattcac tttgccttgc cgtgcgtatt gtttgtctct agcggcgata acgcgttcca
    13201 aaacggacgc gctgctttcg cccgcttcct gctgcatcag ttcggcggcg gacaggctcg
    13261 ggacttcgat ggtcaaatcg atgcggtcga gcagcgggcc ggaaatcttg ctgcggtaac
    13321 gcgcgacgct ttcgggcgtg cagcggcagg gcttgacggg atgcccgaga taaccgcacg
    13381 gacacgggtt catggcggcg acaagttgga atttcgccgg atagacggct tggcgcgccg
    13441 cgcgggaaat gtggatttcg ccgttttcca aaggttcgcg caacacttcc aaaactttgc
    13501 ggtcgaactc gggcaattca tccaaaaata aaacgccgtg atgcgccaat gaaatttcgc
    13561 ccggacgcgg atccgaaccg ccgccgacca tagccgccgc gctggcgctg tgatgcggac
    13621 tgcggaaagg acggttgctg tcgagttgtt gctggtggtt gggcaggagc gaacgcaatg
    13681 cccaaacttc taccaattcg tcttcggtca gcggcggcag gatgccgggc agccgttggg
    13741 agagcataga cttgcccgtt cccggcggac ccatcatcaa gaggctgtgt ccgcctgcgg
    13801 cagcgatttc caaagcaagg cgcgcggtgt gctgaccttt cacatcgcac aaatcaggtt
    13861 gtccgccatg ttcaaacggc atctgaggaa cttggcattc ggtttgcgcc aaaggttcga
    13921 tgccgttcaa atgggcggcg acttcgccca aagagcgcgc gccgtaaacg gtaatgccgc
    13981 gcatcacggc ggcttgtcct gcattttctt caggcaaaac aaatgcacgt tttgcctgca
    14041 taccctgcca cgccatcgcc aacgcgccac gcacggggcg caacagcccc gacagtgcca
    14101 attcccccgc aaactcgtat tcctccagtt tttcgggcgc aacctgcccc gatgcggcaa
    14161 ggatgccgat tgcaatcggc aaatcgaaac gccccgactc tttgggcagg tcggcggggg
    14221 cgaggttgac ggtgattttt ttggcgggga attcaaaacc gctttgaata atggcggcac
    14281 ggacgcggtc gcgactttct ttgacttccg tatcgggcag cccgacgatg ttgaaatgtg
    14341 gcaggccgtt ggcaaggtgg gcttccactt cgaccaacgg cgcattcata ccgctcaagg
    14401 cgcggctgta aaccaaggca agcgacatat ttcagacggc cttattcgcc ggcttcggtt
    14461 tgctgcctga tttcggcgac ggcttcttcg gcagcggctt cagccgcttc caatgctgcc
    14521 cgttcgggat tttgcgcggc ttcgagtttt gccaaacgcg cttccaaagc cgccagtttg
    14581 gtacgagttt tgattaaaac ctgctgctgg atgtcgaatt cttcgcgcgt aaccagatcc
    14641 atacggttga acgcgccgcc cagcatcgcc ttaatatttt tttccacatc tttggcaggg
    14701 ctgttggcga tggtttcgct gattttcgaa ccgacttctt caaaaagctg cttgccgaac
    14761 ataatctgta tccttcctga acatatcaaa ttcaatcggc tattgtataa ggaaaaatgc
    14821 cgtctgaaaa cgggcgacgg ataatcggca aaacataccg cgcctccttt gcggttgcta
    14881 aattacacct caaaacacct gccgcctgaa aacggatttc agattccgcg caacgcctga
    14941 ttacaagaca ctacaaaaca atatgctgtt ttaaatgatt tttccgacgc gcatcgtttc
    15001 aaactcggct ttttaagcca ttaagtgctt tgcaaacaac agaaattggg ttatgctgaa
    15061 acggattatt tacaatttca tatagtttta ttacatatct tattgtgatt gaagataatt
    15121 tatccgaatc cccctttcgg gtatccggat tttccgttgt acttttatta gaaaaacatt
    15181 tcaggcgcaa gttgcttgca atttcaaagc ctgtaattct gaagttttag acgaggaata
    15241 gcacatgaat cccatgtaca ttacttttgc aatctatctg gttgcagtcc ttctcatcgg
    15301 gcttgccgcc tatttttcta cgcgcaattt cgacgattat attttgggcg ggcgcagcct
    15361 aggcccgttt gttaccgcga tgtcggcagg cgcgtccgat atgtccggct ggctgctgat
    15421 gggtctgccg ggcgcgattt atttgagcgg tttgaatgag gcttggattg ccatcggcct
    15481 cttggtcggc gcgtatttca actggctttt ggtggcgggc cgtctgcgcg tgcataccga
    15541 atacgccaac aatgcgctga cgctgccgga ttatttcttc caccgcttcg gcgcgggcgg
    15601 acacttgatg aaagtggttt ccgcattgat tatcctgttc ttcttcacga tttattgcgc
    15661 ctcgggcatt gtggcgggcg caaccctgtt ccaaagccta tttgaaggta tgacttacaa
    15721 tcaggcaatg tggctgggcg cgggcgcgac catcgcctat accttcttgg gcggtttctt
    15781 ggcggtcagc tggacggata cgctgcaggc ttctttgatg attttcgccc tgattttaac
    15841 gccggtgatg gtctatctgg gcttgggcgg cgcggaacag atgtctaccg cgattcaaag
    15901 cgtcgccgca ggcacgggca aagaatacgg cagcctgttt gccggtacga ccgtcatcgg
    15961 catcatttcc accgccgcat ggggcttggg ctatttcgga cagccgcaca ttttggcgcg
    16021 ctttatggcg gccgaaagcg cgaaatcgct ggtatccgca cgccgcatcg gtatgacttg
    16081 gatggcgttg tgccttgcgg gcgcggtagc ggtcggttat ttcggcattg cgtatttcgg
    16141 tgcaaatccc gacaaagttt cttctatgag cggcaaccac gaacgcatct tcatcgcgct
    16201 ttccaccctg ctgttcaacc cttggatcgc cggcatcatt ttgagcgcga ttttggcggc
    16261 ggtgatgtcc accctgtcct gccagctttt ggtttgctcc agcgcgatta ccgaagactt
    16321 ctacaaaggc tttttgcgta aaaacgcgca acagtcggaa ttggtatggg tcggccgcct
    16381 gatggtcttg gcgattgccg tgatttccat cctgattgct tccgatccga acagcaaagt
    16441 attgggactg gtgtcttacg cgtgggcagg tttcggcgcg gcattcggcc cgattgtgat
    16501 tctgtccgta ttgtggaaac gcatcactgc ctacggcgcg ttgtcgggta tggttgcagg
    16561 tgcgttaacc gtagtcgtat gggcggaatg ggtgaaaaaa ccggctcaag cggcaggaga
    16621 aagcggcttg ttgacgatgt acgaaatcgt gccgggcttt attgtttgct tgatcgttgc
    16681 cgtgttggtt tccctgttca acaaagaacc ttcgcgcgaa acccaagaac gctttgaaaa
    16741 agccgacgca gattaccgcg ccacccgata aacccctttc agacggcacg gcatcctggt
    16801 tcgtgccgtc tgaacccgtt tccgggtaaa acacaaataa accttgcctg catacagacc
    16861 ttatttgtgt tttacccgtt tcattttcaa agaaacggaa gcacctaccc tgcccgcatt
    16921 tgaagccttg tatttcatct gcggatttat tacgatatta ccgtattcag gccgcaccga
    16981 tgccgcctgc cccccgaaaa actttggaga atccaaaaaa tgtttcattt tgcatttccg
    17041 gcacaaaccg ccctgcgcca agcgataacc gatgcctacc gccgtaatga aatcgaagcc
    17101 gtacaggata tgttgcaacg tgcacagatg agcgacgaag agcgcaacgc cgcctccgag
    17161 cttgcccgcc gtttggttac ccaagttcgt gccagccgca ccaaagccag cggcgtagat
    17221 gcgctgatgc acgagttttc actctccagc gaagaaggca tcgcgctgat gtgtctggca
    17281 gaagccctac tgcgtatccc cgacaacgcc acgcgcgacc gcctgattgc cgacaagatt
    17341 tcagacggca actggaaaag ccatttgaac aacagccctt ccctcttcgt caatgccgcc
    17401 gcctggggcc tgctgattac cggcaaactg accgccacaa acgacagaca aatgagttcc
    17461 gcactcagcc gcctgatcag caaaggcggc gcaccgctca tccgccaagg cgtaaattac
    17521 gccatgcgcc tgttgggcaa acagttcgta accggacaga ccattgaaga agccctgcaa
    17581 aacggcaaag aacgcgaaaa aatgggctac cgcttctcct tcgatatgtt gggcgaagcc
    17641 gcctacaccc aagccgatgc cgaccgctac taccgcgact atgtcgaagc catccacgcc
    17701 atcggcaaag atgcggcagg acaaggcgtt tacgaaggca acggcatttc cgtcaaactt
    17761 tccgccatcc atccgcgcta ctcgcgcgcc caacacggcc gtgtgatggg cgaactgttg
    17821 ccgcgcctga aagagctgtt ccttttgggt aaaaaatacg atatcggtat caacatcgat
    17881 gccgaagaag ccaaccgtct ggagctgtct ttggatttga tggaggcttt ggtttcagac
    17941 cctgacttgg ctggctacaa aggtatcggt ttcgttgtcc aagcctacca aaaacgttgt
    18001 ccgttcgtta tcgactacct gattgaccta gcccgccgca acaaccaaaa actgatgatc
    18061 cgcctcgtca aaggcgcgta ttgggacagc gaaatcaaat gggcgcaagt ggacggcttg
    18121 aacggctatc cgacctacac ccgcaaagtc cacaccgaca tctcctacct cgcctgcgcg
    18181 cgcaaactgc tttccgcgca agacgcggta ttcccgcaat ttgccacgca caacgcctac
    18241 accttaggcg caatctacca aatgggcaaa ggcaaagact ttgaacacca atgcctgcac
    18301 ggcatgggcg aaaccctgta cgaccaagtc gtcggcccgc aaaacttagg ccgccgcgtg
    18361 cgcgtgtacg ccccggtcgg cacacacgaa accctgctcg cctacttggt gcgccgcctg
    18421 ttggaaaacg gcgcgaactc gtctttcgtc aaccaaatcg tcgatgaaaa catcagcatc
    18481 gacacgctca tccgcagccc gttcgacacc atcgccgaac aaggcatcca cctgcacaac
    18541 gccctgccgc tgccgcgcga tttgtacggc aaatgccgtc tgaactcgca aggcgtggac
    18601 ttgagcaacg aaaacgtatt gcagcagctt caagaacaga tgaacaaagc cgccgcgcaa
    18661 gacttccacg ccgcatccat cgtcaacggc aaagcccgcg atgtcggcga agcgcaaccg
    18721 attaaaaatc ctgccgacca cgacgacatc gtcggcacag tcagttttgc cgatgccgcg
    18781 cttgcccaag aagcggttgg cgcagccgtt gccgcgttcc ccgaatggag tgcgacacct
    18841 gccgccgaac gcgccgcctg cctgcgccgt ttcgccgacc tgctcgaaca gcacaccccc
    18901 gcgctgatga tgcttgccgt gcgcgaagca ggcaaaacgc tgaacaacgc cattgccgaa
    18961 gtgcgcgaag ccgtcgattt ctgccgctac tacgcaggcg aagccgaaaa caccctgccc
    19021 aaagacgcca aagcagtcgg cgccatcgtc gccatcagcc cgtggaactt cccgctcgcc
    19081 atctttaccg gcgaagtcgt ttccgcattg gcggcaggca acaccgtcat cgccaaaccc
    19141 gccgaacaaa ccagcctgat tgccggttat gccgtttccc tcatgcacga agccggcatc
    19201 ccgacttccg ccctgcaact cgtcctcggc gcaggcgacg tgggtgcggc attgaccaac
    19261 gatgcccgca tcggcggcgt gattttcacc ggctcgaccg aagtggcgca cctgatcaac
    19321 aaagcccttg ccaaacgcgg cgacaatccc gtcctgattg ccgaaaccgg cggacaaaac
    19381 gccatgattg tcgattccac cgcacttgcc gaacaagtct gcgccgacgt attgaactcc
    19441 gccttcgaca gcgcgggaca acgctgctcc gccctgcgta tcttgtgcgt ccaagaagac
    19501 gttgccgacc gtatgctcga catgatcaaa ggcgcgatgg acgaactcgt cgtcggcaaa
    19561 ccgattcagc tcactaccga tgtcggcccc gtcatcgatg ccgaagcaca gcaaaacctg
    19621 ttgaaccaca tcaacaaaat gaaaggcgtt gccaagtctt accacgaagt caaaaccgct
    19681 gccgatgtcg attccgaaaa atccacgttc gtccgcccca tcctgtttga attgaacaac
    19741 ctcaacgaac tgcaacgcga agtcttcggc cccgtcctgc acgtcgtccg ctaccgcgcc
    19801 gacgaactcg acagcgtcat cgaccaaatc aacagcaaag gctacgccct gacccacggc
    19861 gtacacagcc gcatcgaagg cacggtacgc cacatccgca gccgcatcga agccggcaac
    19921 gtttacgtca accgcaacat cgtcggcgca gtcgtcggcg tacagccctt cggcggacac
    19981 ggtctgtccg gcacaggtcc caaagcaggc ggttcgttct acctgcaaaa actgacccgc
    20041 atccccgaat gggttgcccc gaccctgagc caaatcggac aggcagacga agccgcgctc
    20101 aaacgcctcg aagcactggt tcacaaactg ccgttcaatg ccgaagagaa aaaagccgca
    20161 gcggccgctt tgggacacgc ccgcatccgc accctgcgcc gtgccgaaac cgtccttacc
    20221 ggaccgaccg gcgagcgcaa cagcatctca tggcacgcgc ccaaacgcgt ttggatacac
    20281 ggcggcagca cggttcaagc ctttgccgca ctgaccgaac ttgccgcctc cggcatacag
    20341 gcagtggtcg aacccgacag ccccttggct tcctacactg ccgacttgga aggtctgctg
    20401 ctggtcaacg gcaaacccga aaccgccggc atcagccacg ttgccgccct gtcgcctttg
    20461 gacagcgcgc gcaaacagga acttgccgcc cacgacggcg cactcatccg catcctccct
    20521 tcggaaaacg gactcgacat cctgcaagtg tttgaagaaa tctcttgcag cgtcaacacc
    20581 acagccgccg gcggcaacgc cagcctgatg gcggtcgccg actgattttg ccgaaatacc
    20641 cgggcgcggc ccgtgaacca atgccgtctg aaaacctttc agacggcatt tttata
//
LOCUS       DAWHRA010000019        20406 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWHRA010000019 DAWHRA010000000
VERSION     DAWHRA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.019"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..2295)
                     /gene="ligA"
                     /locus_tag="WIB30_000523"
     CDS             complement(<1..2295)
                     /gene="ligA"
                     /locus_tag="WIB30_000523"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ3796646.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGAFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLILKADFVALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     PRAPRWAIAHKFPAEEALTVVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEISRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAET
                     PSANQTQSIPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETTDKTVAESDQTPSEG
                     SSVGASGKHKKQPVKWAENILGGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQAQAVTIPP
                     AHHAEPQRWIARLPGFKISENKAQALWELAGQSIEGLQNDKALPTDWQAWRSETQNAV
                     LLENLKTFFAQMPSEDETAQGSDGI"
     gene            complement(2443..3729)
                     /locus_tag="WIB30_000524"
     CDS             complement(2443..3729)
                     /locus_tag="WIB30_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ3796647.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(3923..4495)
                     /gene="ampD"
                     /locus_tag="WIB30_000525"
     CDS             complement(3923..4495)
                     /gene="ampD"
                     /locus_tag="WIB30_000525"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ3796648.1"
                     /translation="MDNPATAPWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            4580..5575
                     /gene="mltG"
                     /locus_tag="WIB30_000526"
     CDS             4580..5575
                     /gene="mltG"
                     /locus_tag="WIB30_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ3796649.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            5635..6255
                     /locus_tag="WIB30_000527"
     CDS             5635..6255
                     /locus_tag="WIB30_000527"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ3796650.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLDRAAACPERYAVIDSNLGLDEVRNSIEKVLDRHFGC"
     gene            6477..7757
                     /locus_tag="WIB30_000528"
     CDS             6477..7757
                     /locus_tag="WIB30_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ3796651.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            7993..8264
                     /locus_tag="WIB30_000529"
                     /pseudo
     CDS             7993..8264
                     /locus_tag="WIB30_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8304..9338
                     /locus_tag="WIB30_000530"
     CDS             8304..9338
                     /locus_tag="WIB30_000530"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ3796652.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAASRAEDAGDEPLLLFSKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            9544..10125
                     /locus_tag="WIB30_000531"
     CDS             9544..10125
                     /locus_tag="WIB30_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ3796653.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            10194..10376
                     /locus_tag="WIB30_000532"
     CDS             10194..10376
                     /locus_tag="WIB30_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ3796654.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            10373..11134
                     /gene="kdsB"
                     /locus_tag="WIB30_000533"
     CDS             10373..11134
                     /gene="kdsB"
                     /locus_tag="WIB30_000533"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ3796655.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            11157..11474
                     /locus_tag="WIB30_000534"
                     /pseudo
     CDS             11157..11474
                     /locus_tag="WIB30_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            11579..11776
                     /locus_tag="WIB30_000535"
     CDS             11579..11776
                     /locus_tag="WIB30_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796656.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            11933..12025
                     /locus_tag="WIB30_000536"
     tRNA            11933..12025
                     /locus_tag="WIB30_000536"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11967..11969,aa:Ser,seq:gct)
     gene            complement(12061..12231)
                     /locus_tag="WIB30_000537"
     CDS             complement(12061..12231)
                     /locus_tag="WIB30_000537"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796657.1"
                     /translation="MKTGKDYAVQFFVYQNSPLFRNLPFRLVKSDFARAGTLQIQAAH
                     GRQPSALPQQSA"
     gene            12328..13113
                     /locus_tag="WIB30_000538"
     CDS             12328..13113
                     /locus_tag="WIB30_000538"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ3796658.1"
                     /translation="MSRIRQAFAALNGEKALIPYITVGDPDIGATLAIMHSMVENGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            13150..14022
                     /locus_tag="WIB30_000539"
     CDS             13150..14022
                     /locus_tag="WIB30_000539"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ3796659.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPIDPLKFKDSKKYPDRLGAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(14082..14693)
                     /locus_tag="WIB30_000540"
     CDS             complement(14082..14693)
                     /locus_tag="WIB30_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ3796660.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHNK"
     gene            14802..15026
                     /locus_tag="WIB30_000541"
     CDS             14802..15026
                     /locus_tag="WIB30_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ3796661.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            15027..15632
                     /locus_tag="WIB30_000542"
     CDS             15027..15632
                     /locus_tag="WIB30_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796662.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            15692..16726
                     /gene="pyrC"
                     /locus_tag="WIB30_000543"
     CDS             15692..16726
                     /gene="pyrC"
                     /locus_tag="WIB30_000543"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ3796663.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(17094..17519)
                     /gene="nusB"
                     /locus_tag="WIB30_000544"
     CDS             complement(17094..17519)
                     /gene="nusB"
                     /locus_tag="WIB30_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ3796664.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(17597..18073)
                     /locus_tag="WIB30_000545"
     CDS             complement(17597..18073)
                     /locus_tag="WIB30_000545"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ3796665.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(18121..18444)
                     /locus_tag="WIB30_000546"
     CDS             complement(18121..18444)
                     /locus_tag="WIB30_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ3796666.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            18611..19330
                     /gene="rnc"
                     /locus_tag="WIB30_000547"
     CDS             18611..19330
                     /gene="rnc"
                     /locus_tag="WIB30_000547"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ3796667.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            19377..20300
                     /gene="era"
                     /locus_tag="WIB30_000548"
     CDS             19377..20300
                     /gene="era"
                     /locus_tag="WIB30_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ3796668.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         4552 a         5007 c         6045 g         4802 t
ORIGIN      
        1 gatgccgtct gaaccttgcg ccgtctcgtc ttcagacggc atttgcgcga agaaggtttt
       61 caggttttca agcaggacgg cgttttgtgt ttcgctgcgc cacgcctgcc agtcggtggg
      121 aagggctttg tcgttttgca gcccttctat actttgaccg gcgagttccc ataaggcttg
      181 ggctttgttt tcgctgattt tgaaaccggg caggcgggca atccagcgtt gcggttcggc
      241 gtggtgggcg ggcgggatgg taacggcttg ggcttgcggg gcaacgcctg cggcgagcag
      301 ctcgtctatc atcgcctgct gttcggcttg ggcgaagaag tgggcaatgg aatgcgccac
      361 taccgtaccg atgtcgggca ggcaggcgag gacgggttcg ggggcgcggc ggacgcgttc
      421 caatgtgccg aatgcctgcg ccagcgtttt ggcggtgcgt tcgccgacgt ggcggatgcc
      481 gagcgcgaac aggaagcgcg cgagttcggg cgttttgccg gattctatgc ccccgaggat
      541 gttttccgcc cacttgaccg gttgtttttt atgtttgccc gacgcgccga ccgaactgcc
      601 ttcagacggc gtttgatccg attcggcaac ggttttgtcc gtcgtttctt tcattttttg
      661 cagggtcgga atatcgaggc ggtagaggtc ggcgaagtgg cggacgaggt cttgggcgac
      721 aagctgttcg atttgttttt cacccaagcc gtcgatgtcc atggctttgc gcgaggcgaa
      781 gtggattaag ccttgcgcgc gttgtgcctg acaaagcata ccgccgctgc atcgggcgac
      841 ggcttcgcct tcttcgcgtt cgatttcgct gcggcagatg gggcaatggg tcggcaggcg
      901 gtagggcttg tggagcggaa tggattgggt ttgattagcg gacggtgttt cggcaaacaa
      961 atcgtcctgc cgatgcccga tgccgtctga aacagcaacg gcggtttcct gcatcgggcg
     1021 gcgttcaaaa atcacacgca cgacttcggg aatcacgtct ccggcgcggc gcacgacgac
     1081 ggtatcgccg acgcgcacgt ctttgcgcga tatttcgtcc tgattgtgca gggtggcgtt
     1141 ggtaacagtt acgccaccga cgaatacggg ctgtaatcgg gcaaccggcg ttaccgcgcc
     1201 cgtcctgccg atttgcacgt caatcgcttc gacgacggtc agggcttctt cggcagggaa
     1261 cttgtgggca atcgcccagc gcggcgcgcg ggggatgaag ccgagttcgc gttgttgcgc
     1321 caagctgttg actttgacca ccatgccgtc gatttcgtag ggcagttcgg ggcgtttttg
     1381 ctgcatgtgt tcgtaaaacg ccaatacttc gtcgatattt ttgaaacagc cgaaattgcc
     1441 attgggcaga ctgaagccga gtgcttggaa ataggcgagt tcctggatgt gttcttccgc
     1501 gacgaaacca tcttgctggc gggcgacgga gtaggggaaa aagtgcagtt tgcgttgcgc
     1561 ggtgatgcgc gaatcgagtt ggcgtaggct gccggcggcg gcgttgcgcg gattggcaaa
     1621 gggtttttgc ccgttttcgg cttgtctttt attgagggcg acaaaatcgg ctttgagtat
     1681 cagcacttcg ccgcgtacct cgatgagttc gggcgtattt tcgccgtgca gccgcaaggg
     1741 gatgttggat acggttttga tgttttgggt aacgtcttcg cccgtcgtgc cgtcgccgcg
     1801 cgttgccgcc tgcaccaata cgccgtcgcg gtagagcagg ctgatggcga ggccgtcgaa
     1861 tttgggttcg ataacgtatt cgggattgcc gccgtccaag ccgtcgcgca cgcgttggtc
     1921 gaaggcgtac atttcggcat ggtcgaacgc gccgttttca tcttgcgggg aaaaagcgtt
     1981 ggtcagcgac agcatcggca cttcgtggcg tacttcggca aatcccgcca aaggctcgcc
     2041 gccgacgcgc tgggtcgggc tgtcgggcag tttgagctcg ggatggttta actccaacgc
     2101 ttcgagttcg cggaacaatt tgtcgtattc ggcatcgggt acgctgggcg cgtcgagggt
     2161 gtagtattcg taggcgtagc ggttgaggag gtcggtgagg cggcagatgt gttgtgcggc
     2221 aaattgtttt atatcactat cagacggttt aagaagattg gtaaagttag tgttatgttt
     2281 tgagtttgga ttcatgagag aaggttttca gacgaccttt gtctgatacg ggatgaaacg
     2341 ggcaaaggtc gtctgaaaaa tgataggttg aaaacagctg aattttaccc gaaaaaaagc
     2401 ggatatgccg taacgacata tccgctttga ttgcattcga ttttaggaga acaggcgcaa
     2461 tgcggttttg ccgcccggtt cgataccgac tttgagcatc tcggactgac gagccaatac
     2521 ataagtgcgc acgtctttga gccattgggt cgaaacttct tccattttgt cgttgaccag
     2581 attcaggttc aactggccgg acaggcgtac cgccaaatcc ataaacaaat cgtcgaaggt
     2641 tttttcgcct gccggagagt gcgggatgtc gagcagcata ctgaagcctt tataggattg
     2701 gttgtccaaa agggcgttgg taaacggctc gttgttgagc gagcagatgg agaacatggt
     2761 cgaaccggac gtgtcggtat agtggaacgc gccgtcgtct tccaaaacga aacccacgcc
     2821 cgttacggcg gaacgcagtt ctacgccgct gatgctggtc ggggaaacca aatggatggc
     2881 gatggtctgg tcgacgcgcg cgcagaatgc gtccagtgcg gaagccactt cgataaaggc
     2941 ggcaaggtcg gtgtgcagcg tctgaccgcc catgctttgt gcgaatgcgt ccacctggcg
     3001 gttgaatgcg gagagttctt cctgcgaggc aagtccgttg cggctgactg cctgaatacc
     3061 cacgataaat gcctgatagc ggatgcccgg gatgggttcg gcaatctgga aatggtcgtc
     3121 catggtgcag ccgacaatct ggtagcggca gcggttggaa aggcgcggca gtgcgtgcag
     3181 ttctttggct tcggtcagcg cgatatagga gatgaagtcg aagcgcacgt caaaccaggg
     3241 cagctcgacc ttcgacagct ctttgagcgt aatcagcggt tttgccggcg tttgcggaac
     3301 gggaacgggt tttgccggcg cgtcggcagg tttcggtgcg gaatgtccgg tttggggttc
     3361 ggaaacggtg tgggcggagt tgccgataat gccgctttct tccaaggcgg tttcgatttc
     3421 ggttttgaac ggtgatgctt ttgcctgttt ctgcttggcg atgtagacgg catcctgttc
     3481 ttgcaggttg cgcatggcgg ggtcttgggg ttttgccgtt tttttgaccg ccggttgggg
     3541 tttcggcatc atgactgacc cgccggacgg tttgccgtcg cggacatggc tggttttgct
     3601 gttgagcagg gcatctttgt cggagtgtcc gaactggtcg cgcacttttt tgcggtattg
     3661 gttttcctga tacatattgt aggcgacaac ggcgaggacg gcggcgagga acagtacgat
     3721 gtaaatcatg gcaatcactt taaatttcgg gatgcaggat gcgcgaagtg cgggtactgc
     3781 ggttaaatcg ggcttgcgtt tccggcagtc tgatggaacg gccggttata acgtttgaat
     3841 tataacgaaa attgcagggt ctgacagcag tgtgtcgaaa taagcggaaa ttttccgaaa
     3901 tgccgtctaa aatctgtggt tttcagacgg catttctgtc cacgggaaac cctttccccc
     3961 gtatccgccg ccagtcgaaa aaatgaccgg ggtcggtttt gcggccgggc gcgatgtctt
     4021 ggtgccccgt taccgccgtg atggggtagc ggcggcagat tgcgtccaac aaggcttcga
     4081 gcgaacggta ttgcgcttcg gtaaagggtt cgaaatcgca gccttccagt tcgatgccga
     4141 tggaaaatgc gttgcatttt tcccgtccgc gaaacgagga tacgccggca tggtatgcca
     4201 tattgtcgca ggaaacgaac tgtaccgttt cgccgtcgcg tttgattaaa aaatggctgg
     4261 atacgcgcaa agtgtgtatc aggctgaaga acggatgtcc gtcggggtcg agccggttgg
     4321 caaacagctt ttccaccgca tccgtaccat attcgaacgg cggcagcgag atattgtgca
     4381 atacgacaag ggaaacctct tctttggact ccctcgggcc gaaattcggc gacggaatgt
     4441 ggcgcacgct ttgcagccaa ccgtcctgcc agggtgccgt agcgggattg tccatgatgt
     4501 cctttctgtc ttatggggta atttgggtta tactgtcgcc cgaattttaa gacgtattcc
     4561 gaatgctggg aatcctacca tgttgagaaa attgttgaaa tggtctgccg tttttttgac
     4621 cgtgtcggca gccgttttcg ccgcgctgct ttttgttcct aaggataatg gcagggcata
     4681 ccgaatcaag attgccaaaa atcagggtat ttcgtcggtc ggcaggaaac ttgccgaaga
     4741 ccgcatcgtg ttcagcaggc atgttttgac ggcggcggcc tacgttttgg gtgtgcacaa
     4801 caggctgcat acggggacgt acagactgcc ttcggaagtg tctgcttggg atatcttgca
     4861 gaaaatgcgc ggcggcaggc cggattccgt taccgtgcag attatcgaag gttcgcgttt
     4921 ttcgcatatg aggaaagtca tcgacgcaac gcccgacatc gaacacgaca ccaaaggctg
     4981 gagcaatgaa aaactgatgg cggaagttgc ccctgatgcc ttcagcggca atcctgaagg
     5041 gcagtttttc cccgacagct acgaaatcga tgcgggcggc agcgatttac ggatttacca
     5101 aaccgcctac aaggcgatgc aacgccgcct gaatgaggca tgggaaagca ggcaggacgg
     5161 gctgccttat aaaaatcctt atgaaatgct gattatggcg agcctgatcg aaaaggaaac
     5221 agggcatgaa gccgaccgcg accatgtcgc ttccgtcttc gtcaaccgcc tgaaaatcgg
     5281 tatgcgtctg caaaccgacc cgtccgtgat ttacggcatg ggtgcggcat acaagggcaa
     5341 aatccgtaaa gccgacctgc gccgcgacac gccgtacaac acctacacgc gcggcggtct
     5401 gccgccaacc ccgattgcgc tgcccggcaa ggcggcactt gatgccgccg cccatccgtc
     5461 cggcgaaaaa tacctgtatt tcgtgtccaa aatggacggc acgggcttga gccagttcag
     5521 ccatgatttg accgaacaca acgccgccgt ccgcaaatat attttgaaaa aataaaccat
     5581 gccgtctgaa aagtttgtgt tttcagacgg cataccctta ccggaactgc aagcatgaaa
     5641 ccgcaattca tcactttgga cggcatagac ggtgccggca aatccaccaa ccttgccgtc
     5701 atcaaggcat ggtttgaacg gagggggctg cccgtgctgt tcacgcgcga accgggcgga
     5761 acgccggtcg gtgaggcctt gcgcgaaatc ctgctcaacc ctgaaaccaa agccggtttg
     5821 cgtgcggaaa cactgatgat gttcgccgcc agaatgcagc atattgaaga cgtgatattg
     5881 cctgcacttt cagacggcat ccatgtcgtg tccgaccgtt ttaccgatgc gaccttcgcc
     5941 tatcagggcg gcgggcgggg aatgccgtct gaagacattg aaattttgga acattgggtg
     6001 cagggcggtt tgcgccccga tttgaccctg ctgttggatg tgccgctgga agtgtcgatg
     6061 gcgcgtatcg ggcagacgcg cgagaaagac cggttcgagc aggaacaggc agatttcttc
     6121 atgcgtgtgc gcagcgttta tctcgaccga gccgccgcct gtccggaacg ttacgccgtt
     6181 atcgacagta acctcggctt ggatgaagtc agaaacagca tagaaaaagt gttggacaga
     6241 catttcggct gttgatgcgg caaatattga aacaagcgca tccgcccgcg ccgaaaatca
     6301 aacggcagtg ccgcaggtga aaatggcggt atgcgccaaa ctttcggcat gatagaatta
     6361 cgctcggtta caaggcagga tgcgtcggca atattaacga accgcccgta acatgatgac
     6421 ccgaaagcgt ttcggacagt ccgattcaaa tctttttctc gcaacaggat tgacacatgg
     6481 aaaactcatt gaaagaagcc gccctcaagt tccacgaatt acccgttccg ggcaaaattt
     6541 ccgttacccc gaccaaatct ctggcgaccg acaaagattt ggcgttggcg tactctccgg
     6601 gcgtagccgc tccttgtatg gaaatccatg ccgatccgca aaatgcctac aaatacaccg
     6661 ccaaaggcaa cttggtcgct gtcatttcca acggtacggc cgttttgggc ttgggcgaca
     6721 tcggcgcgct ggcgggcaaa cccgtgatgg aaggcaaagg cgtattgttc aaaaaattcg
     6781 ccggtgtgga cgtgttcgac atcgaaatcg atgaaaaaga cccgcaaaaa ctcgtggaca
     6841 tcatcgccgc tttagagccg accttcggcg gcatcaacct cgaagacatc aaagcacccg
     6901 agtgtttcta catcgaacgc gaattacgca aacgctgcaa aatccccgta ttccacgacg
     6961 accagcacgg cacggccatc attaccgccg ccgccgtatt gaacgctctg cgttttaccg
     7021 gccgtaaaat cgaagaagcg accttggttt gctctggtgc gggtgcggcc gcgattgcct
     7081 gcttgaacca attgctggat ttgggcttga aacgcgaaaa cgtaaccgtt tgcgactcca
     7141 aaggcgtgat ttaccaaacc cgcgaagaca aagatcgcat ggacgaatcc aaaaaattct
     7201 acgccgttga agacaatggc cagcgcgtac ttgccgatgc cgtcaaaggt aaagacatct
     7261 tcttgggcct ctccggcgcg aacctgctga cccccgaaat gttgaacacg atgaacgaaa
     7321 aacccatcgt gttcgccatg gccaacccga atccggaaat cctgccgccg ctggcaaaag
     7381 aaacccgtcc ggacgtggtt atcggtaccg gccgttccga cttcccgaac caagtcaaca
     7441 atgtattgtg cttcccgttc atcttccgcg gcgcgttgga tgtcggcgcg accaccatca
     7501 acgaagaaat gaaacgcgcc tgcgtgtatg ctttggcgga tttggcgatg gaagaagtaa
     7561 ccgaagaagt ggttgccgct tacggtaaga aatttgaatt cggcgcggaa tacctgattc
     7621 cgactccgtt cgattcccgc ctgctgccgc gcgttgctac ggctgccgcc aaagcagcga
     7681 tggaaagcgg agtggcaacc cgtccgattg cagatttgga agcttacgct gccaagctga
     7741 gcgaatggaa gctgtaagcc gtttgcggtt taaaatgccg tctgaactgt tttcaggcgg
     7801 cattttgctg tcagattgat atagtggatt aacaaaaatc aggacaaagc gacgaagccg
     7861 cagacagtac aaatagtacg gaaccgattc acttggtgtt tcagcacctt agagaatcgt
     7921 tctctttgag ctaaggcgag gcaacgccgt actggttttt gttaatccac tataaatgaa
     7981 agatactgga aaatgaaaga gatgaaacct gtccgttatc atattggcga tatgcccgag
     8041 acttcaaaac aaaccgcctc ccgtcatgac gacagggcag tgggtgttga cgatgattgt
     8101 tttcatgatt cctttggtca atttttgttt gggtgttcgg cagaggcaac ccgaaccgcg
     8161 ccaatttctg taaagcgcag ttgcttattt acctgattgg ttcgcttatc ggtttggtct
     8221 tcgcgttgtt tataggtggg tctgtatcag gtacgcatga ttaatgcccc gggctgattt
     8281 tgcttcgagg atttgtatcg aatatgccga attgtttcaa atttcatacc gttatcgaac
     8341 ggcattggca aaaaccttat ccggttttgt cttttctgct taagccgctc tccgggctgt
     8401 ttgccaaaat tgcggcaaaa cggcggacgg attttttatc gggaaaacgg caaagcgaaa
     8461 agctgcccgt gcctgtggtc gtggtcggca acatccacgc gggcgggacg gggaaaacgc
     8521 cgattgttgc cgcgctggtg tcgggtttgc aggaaaaggg cgtcaaggtc ggcatcatca
     8581 gccggggcta cgggcgtaag agcaaggcgg ttcatgtatt gaatgccgca agccgagcgg
     8641 aagatgcggg cgacgagcct ttgctgctgt tcagcaaaac cggtgcgccg acggcggtgg
     8701 gcagcagccg tgcagaggca ggcagggcgt tgctggcggc gcatcccgac atcggactga
     8761 ttgtggcgga cgacggtttg cagcattacg ccctgcggcg agatgtggaa atcgcggtgt
     8821 ttccggcggc ggatacgggg cgcacggatt tggatttact gcccaacggc agtttgcgcg
     8881 aacctttgtt gcggctggat tcggtggatg cggtcgtcgt cagcggcggc aaggcggatg
     8941 cgctgtttag gccgtctgaa aatatgtttc acagccgtat cgaagcggga cggatttacc
     9001 gtttgaacaa tccgtccgaa atactggaca caggccgtct gaaaaatcaa accgtcgtcg
     9061 ccgtggcagg tattgccaag ccggcgcgtt ttttcgattc gttgcggaat atgggcatta
     9121 ccgtgaagcg aaccgtcgcg ctgcccgacc acgccgacat ttcggcggca gatttgcccg
     9181 atgcggacgc ggtcattatt acggagaaag atgcggtcaa attttcagac ggcatttgca
     9241 ccgataatgt ttgggtgttg cccgtttgtg cgataatcga acctgatttg gcggcgtttg
     9301 tgttggagcg gttggaagat gtaccgaagg ccgtctgaaa gcacggtttg ggcggagtga
     9361 ttacggattt gaataagaac gcctcgcgcc gtcattcccg cgcaggcggg aatctaagtc
     9421 tcgaattttc aggaatgcct aggaggcttc agaaatccca aatctccgga tttccacttg
     9481 gacaggaatg agaaaatcgg tcgtattttt tatctgcatt aatcattcgt taaagtattg
     9541 aatattaaac tgaaaacctt gttattgccc ttcgccacgc tggcattgtg caccaacgct
     9601 tttgccgccc cgcctagcga cgcgtcgttg gcgcgttggc tggatacgca gaattttgac
     9661 cgggatatag aaaaaaatat gattgagggc tttaatgccg gatttaaacc gtatgcggac
     9721 aaagcccttg ccgaaatgcc ggaagcgaaa aaagatcagg cggcagaagc ctttaaccgt
     9781 tatcgtgaga atgttttgaa agatttgatt acgcccgaag tgaaacaggc tgtccgtaat
     9841 actttattga agaatgcccg tgagatatac acgcaagaag aaattgacgg catgattgcc
     9901 ttttacggtt cgcctgtcgg tcagtccgtc gttgccaaaa atccgcgctt aatcaagaaa
     9961 tcgatgagtg aaatagcagt atcttggact gcattgtcag ggaaaatcgc gcgacatcat
    10021 ctgcccgcgt ttacggaaga gttgcggcgc atcatctgcg gcggtaaaaa tcccgatgcg
    10081 ggctgtaaac aagccggaca ggttgggaaa aggcatcaga aataaatgat agccgtctga
    10141 aatattgaag agggcatccg attgattgaa ccatcaaacc cgaaagcaac cctatggaaa
    10201 aaaaattctt agacatcctc gtctgccccg ttaccaaagg caggctggaa tatcatcagg
    10261 acaaacagga attgtggagc cgtcaggcga agcttgccta tccgattaaa gacggcattc
    10321 cctatatgct ggaaaacgaa gcgcgtccgt tgggcgaaga ggaactcgaa gcatgaccga
    10381 attcgtcgta ttgattccgg cgcggctgga ttcgtcgcgc ctgcccggaa aagccttggc
    10441 ggacatccac ggcaaaccga tggtcgtgcg cgttgccgaa caggcggcaa aaagtaaagc
    10501 tgtgcgcgtc gtcgttgcca ccgaccatcc cgatattcag acggcctgtc aggcgcacgg
    10561 cgtcgaagtc gtcatgactt caaaccggca cgaaagcggc acgacgcgcc ttgccgaagc
    10621 ctctgccgcg ctgaagctgc cgccgcatct ggtggttgtc aacgtacagg gcgacgagcc
    10681 gctgattgca cctgaactca tcgaccgcac cgccgaagta ctcgtcgaaa acaacgtcca
    10741 aatggcgacc gccgcccacg aattgcacga tttcgacgaa ttgatgaatc ccaatgccgt
    10801 caaagttgtc ctcgacaaaa accgcaacgc catctacttc agccgcgccc cgattcccta
    10861 tccgcgcgat gcgatgcgtg caggaaaacg cgaaatgccg tttgaaaccg ccgtcctgcg
    10921 ccatatcggc atctacgcct accgcgtcgg cttcctgcaa cgctacaccg aaatgagcgt
    10981 ttcgccgctg gaaaccattg aatcgctgga gcaactgcgt gtcctgtggc acggctaccc
    11041 gattgccgtc gaaaccgcca aagaagctcc tgccgccggc gtagatacgc aggaggattt
    11101 ggacaggatt cgcgctgtat ttcagactat ataaaataga ttcaaaggga aaagatatgc
    11161 agcaacatat tgaaaaatgg caacacttga gccgggaaga acagaaaatc cttgctgaag
    11221 tatggggtct cgtgcaaaac gacgatcagg aggttcacta tgaaatgctc aaattgaacg
    11281 cacccgacga agccagtggc gaattttggt tcagaatggc agaaacactc agcaccctgc
    11341 cgcccaaccg ttccctcgac cttagaatga acggcggcag gctggcgacc gccgtatcca
    11401 tcctttccgt catgattgaa gacaatcccg acataccgca gctttgggcg caaaaaatta
    11461 ccgcgctcaa ttatagtgga ttaaatttaa accagtacgg cgttgcctcg ccttgtcgta
    11521 ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatattt
    11581 ggcacacgga cacaaagccc gtgccgacgg tttggcacaa cagcccgaca aagcggcaga
    11641 agccaacgag gaggaatacc tgaccaaagc cctgtcgcaa aacctactgt caacattgga
    11701 tgccgcgctt gcacgttttc ctgaagacgc gtggtttcag gaaatcaaac aggatgcaca
    11761 aaagcatttt gcttgaggat gtggcagtca ggaatatttc cattcaggaa gaaaagaagt
    11821 gcctgattgg gtataatcag ggtaaatctt attttatttc aaaagattaa tatttgcttt
    11881 ctgtttttcc ttgacggtat cggaaaagtt gattatagtt acagcttcct taggagtaat
    11941 ggctgagagg ctgaaggcac ttccctgcta aggaagcatg tggggtcaac ctgcatcgag
    12001 ggttcgaatc cctcttactc cgccaagatg agaagacgct gtgttttaca gcgtcttttt
    12061 ttacgcgctt tgttgcggaa gtgccgaggg ctgtctgccg tgcgccgcct ggatttgcaa
    12121 tgtgcccgcc cgtgcaaaat ccgatttgac caacctgaag ggcaggtttc ggaagagcgg
    12181 ggaattttga taaacaaaaa actgcacggc ataatctttc ccggttttca ttttccgtat
    12241 tcgcctatcc gtaccgcaaa tgctatactg ggaaaaattt accgattgtg ttttacggca
    12301 tatttgccga taggatggaa gagacaaatg agcagaatcc ggcaggcttt tgccgcttta
    12361 aacggtgaaa aggcattgat tccttatatt acggttggag acccggacat tggggcgact
    12421 ttggcaataa tgcacagtat ggtggaaaac ggtgcggata ttttggagtt gggcgtgccg
    12481 ttttccgatc cgatggcgga tggaccggtt attcagcgtg cggcggagcg ggcgttggca
    12541 aacgggattt cgctgcgcga tgtcttggat gtcgtcagaa aattccgtga aaccgacacg
    12601 caaacgccgg ttgttttgat gggatatttg aaccctgtac ataagatggg ttatcgggag
    12661 tttgctcagg aagccgcaat ggcgggtgtg gacggcgtgt tgacggtgga ttcccctgtc
    12721 gaaaccatcg atccgctcta tcgcgagctg aaggataacg gggtcgactg tattttcctg
    12781 attgcgccga cgacgacgga agaccgtatt aaaaccattg ccgagctggc aggcggattt
    12841 gtctattatg tttcgctcaa gggcgtaacg ggcgcggcaa gtttggatac ggatgaggtt
    12901 tcgcgtaaaa tagagtattt gcatcagtat atcgatattc ccatcggtgt cggtttcggc
    12961 atcagcaatg cggaaagcgc gcgcaaaatc ggccgggttg ccgacgcggt cattgtcggc
    13021 agccggattg tgaaagagat tgaaaacaat gcgggcaacg aggctgcggt cgtcggtgct
    13081 ttggtcaaag agttgaagga tgccgtgcgc tgattctgtc acgcattcta aatattttag
    13141 gagttgtcca tgagctggtt agataaaatc ctgccaccca aaatcaaaaa ccgcagcaaa
    13201 gacggttctt ccaatgttcc cgagggtttg tggcacaaat gtccgtcttg ttcggcaacc
    13261 gtttattcga ccgagttgca gcagaacaat caggtttgtc cgaaatgcaa ccaccacaat
    13321 ccgttgtcgg cacgacaacg cctgaacctg cttttggatg aggatggcag ggaggaagtt
    13381 gccggaaatg tcaaaccgat agatcctttg aagtttaaag acagtaaaaa atatcccgac
    13441 cgtttggggg cggcacgcaa gctgaccggg gaagatgacg cgctggtggt gatgaagggg
    13501 atgatgaacg gcctgcccgt cgtcgttgct gcgtttgaat tccgctttat cggcggttcg
    13561 atgggttcgg ttgtgggaga gcgtttcgta cagggcgtcc gccgtgcggt tgccgacaat
    13621 tgtccgttcg tctgtgtggc ggcttccggc ggtgcgcgta tgcaggaggg tgtgaactcg
    13681 ttgatgcaga tgacgaaaac cagtgccgcg ctgcatttgc tgacggaaaa acgcctgccg
    13741 tttatatcgg tattgaccga tccgactatg ggcggcgtat ccgccagctt cgcatttttg
    13801 ggtgatgttg tgcttgccga accgaacgcg ctgatcggtt ttgccggtcc gcgcgtgatt
    13861 gagcagacgg tgcgcgaaac gctgccggaa ggcttccaac gcgccgagtt cctgctggaa
    13921 aaaggcgcaa tcgaccagat tgtcgaccgc cgcgatatga agcggcgcat cagtgatttg
    13981 attacgctgt tgtgccgtca ggacaaagtt tccgccgctt gatggctgat gaatcgaata
    14041 ccgtctgaaa ccgatgtttc agacggtatt tttgtgtctg gttatttgtt gtgcggcttc
    14101 atcgacgggg catagcgtcc ggcacgttct ttcaggcgtt gtaccaaacc tttcgtgtcg
    14161 gcgggtacac cgccctcgca gaatgcctga tacaggacgg tgcgcagtgc gtcgttgcgg
    14221 cttaatgtac cgcctatcgg tttccattcg gcgtttcggg gctgtatcca gcggcggttg
    14281 accgtgtcgc cgtagccgaa cactttatag gaggaaggtt tgccgttgcc gaacctgatg
    14341 gagaagcggg cgcagaatat gccttcggca gtcaggttgt cgtagccttt gtcggagcgg
    14401 atattgagaa tgtagcggat gctgccgtcg ggcgcgggca taatttgcag gctgtcgagc
    14461 aggattttcg gctgtttgcc gtaattttca tccacataaa tgtcgaacca gccgtccgag
    14521 tgcgtatcgg gcagcggcgg cagtttggcg gtatgttctt taaattcgcg ggcggcggcc
    14581 tcttcgggcg tttcgcggta gcgggtgttg atcggcgtgt ctttttggct gaagccggca
    14641 gcgagggacg tgccggcggc gagtgcgagc agcaggaggg cggtgcggcg cataagtttc
    14701 tccaaattga aaacggcgtt attttatggg ttggcaaagg gggctgcaag caactggggt
    14761 ataatctccc cccgattccc attttttaaa cggtacaaac gatgaacagc gaaactttag
    14821 acgtaaccgg attgaaatgt cccctgccga ttttgcgggc gaaaaaggct ttggcgcaaa
    14881 tgcagcaggg cgacgtgttg accgttctgg caaccgacgg cggcgcaccg ggggattttg
    14941 aggctttttg ccgccaaacc ggtcatgtgc tgttggatgc ttccgaacag gacggcgtgt
    15001 tcacgctggt cgtccaacac aaataaatgc cgtctgaaat gcggatgtcc cgattgatgt
    15061 tattgctgtc ggcatttgtg cttgctgcgt gtaatgccca aaaccggcaa tccggtgtac
    15121 ggcatcagga tttcgcgcgt gtagccgtcg atttgtttcg gagtgcctgc gtcctgactc
    15181 aaggcagttt tgaatcggtt tccaagtttg ccgctgtcgg acattttgtc cctgtcggaa
    15241 aggaagacct gtcccatctg ccgcccactg ttgcagaacc cgatgtacag gcgttgtgga
    15301 cgcttgaacg cgatggcggc atttattatt tgagcctgac gcgcgacagt tgcagtgtga
    15361 aagccgagcg ggcagacagt gccgcgctgt tggaacattt tgacggactt gtccgacagc
    15421 cgccgaaaaa tgccaatata gaattgaggg cggaacagtc ggtgcaggca tcatttgaaa
    15481 tccgccaagt cagctatgcc tggcgggaag cgggcagctc cgaagaaacc gtattgaccg
    15541 cacaaaccgc cgattccccg gatttacccg tacaggcagt tttgaacctg acgcaccgtt
    15601 cccacaacgg gaaaccccta attctcccct aacttcagac ggcataaagt cggcatgccg
    15661 tctgaacatt caaataaaac aaaggaaaac catgcaaacc ctgaccatta tccgccccga
    15721 cgatatgcac ctgcacctgc gcgacggcga cgcgctcaaa gccgttgctc cttataccgc
    15781 ccgccaaatg gggcgtgccg tcattatgcc caacctcaaa ccgcctgtcg tcagcgttgc
    15841 cgacgcgctt gcctacaaag cgcgcatcat ggcggcattg cccgaaggca gcgcgtttga
    15901 gccgttgatg acgctttacc tgaccgacca agccacgcct gcacttgtgc gcgaagccaa
    15961 agccgccggc atcgtcgcct tcaaactcta ccctgccggc gcgaccacca attccgattc
    16021 cggcgtaacc gacctgttca agctcatccc cgtgttggaa gaaatggcga aacagggcat
    16081 tttgttcctc gttcacggcg aagtaaccga ccccgaaatc gacatcttcg accgcgaagc
    16141 cgcctttatc gggcgcgtga tgaaacccgt tttggcgcaa gtgccgaatc ttaaagtcgt
    16201 gttcgaacac atcaccaccg ccgaagccgc ccgcctggtt ttggaagcag gcgacaacgt
    16261 agccgccacc gttaccccgc aacacctcct gctcaaccgc aacgacctct tggtcggcgg
    16321 cgtgcgcccc catcatttct gcctgcccgt actcaaacgc gaaacccacc gtcaggcatt
    16381 ggttgccgcc gttaccggcg agaaggcgca caaattcttc ctcggcaccg actccgcgcc
    16441 gcacgccaag tccgccaaag aaaacgcctg cggctgcgcc ggtatgttca gcgcgatgac
    16501 cgccgtcgag ctttacgccg aagtgtttga aaaagcaggc gcgttggaca aactcgaagc
    16561 ctttgcctcg aaaaatggcg caaggtttta cggcattccg gaaaacgccg gcacgatcac
    16621 cctcgtcaaa caaagccaaa ccgtccccgc aagtgttccc tacggcgacg gcgaacttgt
    16681 cccgatgcgc gcgggcggcg aaatcggctg gacggtgcag tattgatggg ctggaaacaa
    16741 aatgccgtct gaagggaggg ctttcagacg gcatttgtgt tgctgactga ttgatacgtc
    16801 aacggcgttt gagtttgtta cgtttcggtt atttccgata aattcccaca attttcaaat
    16861 ttcgccattc ccacgaaggc aggaatccag aaattcgatg cgaccaaagt ttatcaaaaa
    16921 cggcagcaac tcaaaaaacc ggattcccgc ctgcgcggga atgacgagat tgaagtttca
    16981 gaatttattt gaaataccca aaattcaaaa aaccaaattc ccacctgcgt gggaatgacg
    17041 aaacaaagaa agcagaaata aggacataga actttcttta aatttgtgat gcatcaacgg
    17101 cgtttgggct cgtcggggcg gatttgggcg gcgagtttgt cgaggatgcc gttgacgaat
    17161 ttgtgcccgt ccgtgccgcc gaaggttttg gtaacttcga tggcttcgtt gatgatgacg
    17221 gggtagggtg tttcgggcat ggcggacagc tcgtggcagg cggtcagcag gacggcgcgt
    17281 tcgatgggat tgaggtcttt ttcgtcgcgg tcgagcaggg ggcggatttt ttggatgtag
    17341 tccgctgcgt tggtttgcgt accgaagaaa agtttgttga acaattcttc gtccgctttg
    17401 gcaaagtcgg gcatttcgcg gatgttttta gcaatttcgg gcgcggcggt gcggttgata
    17461 agggattggt aaacggcttg tacggcaagc tcgcgggaac ggcggcgggc tgttttcatg
    17521 atttttcctt gaaacggttg ggcggcacgg tatgccgtct gaaacggaaa gggcgtattg
    17581 gtgtacgccc tgtttgttat tcttcgtctt caaactgttc ttcgagcagc aggttgacga
    17641 ggttggcgca ttcgacggcg actttggcgg catccgaggc tttttcttca atccgttcga
    17701 ttgcctgcgc gtcgttttcg gtggttagga cggcattggc aatcgggatg ttgtagtcga
    17761 gtgcgacgcg gctaacccct gctccggatt cgttggaaac cagctcgaaa tggtaggttt
    17821 cgccacggat gacgacaccg atggcaatca gtgcgtcaaa cttttcggaa gaggcaaggt
    17881 tcatcagtgc gatggggatt tcaagtgcgc cgggtacggt ggcgacggta atgttttcgt
    17941 ctgccacgcc caattcttgg agggtgcggc agcagacttt gagcatttcg ctgccgattt
    18001 cgttggtgaa acgtgcctgt acgatgccga tgcggaggtg tttgccgtcg aggttggggg
    18061 cgatggtgtt cattgggtgt cctttggtat tcggaggttt cggaatgccg tctgaaggtt
    18121 tcagtcttgc ggctgccagt cggcgacggt ttggaatgtg ccgtcttcgg caagctccca
    18181 tgcgctgcct tcgggttggg aaagcagtgc ggcggtttca gggttggttt tggcgatgtc
    18241 ggcgaggctg acgatgctga agttgtcggg atcgtcggtg tattcgtcgg tctcgtcgcc
    18301 gctgaagaaa cgccagccgc tgtcgttttc aaaaacgggg gcttcgcggt agaggaagcc
    18361 gacgggccgg ttttgtttgg cgacggtgtt ggtggcgata cagcggtcga gtgccgagga
    18421 aagtgcttgt gcaaatgcgt tcattacggg aatacgttgg gggaaaactt acggatttta
    18481 ccacgattcg tgcgttgtcg gcagacggcg gcggtttggt ggtacaatgt gcgccgtttg
    18541 cagccttaag gtgtttctgt atttttggag tatggaaacg cattcgggct gttttttgcg
    18601 gaagacggta atgaaagacg atgttttgaa acagcaggca cacgcggcga tacagaagag
    18661 actgggctac gcgttccgcg atatttcgct tttgcggcag gctttgacgc acaggagcca
    18721 tcatgcgaag cacaacgagc ggttcgagtt tgtcggtgat tcgattttga attatacggt
    18781 ggcgcggatg ctgtttgaca cgtttccgaa gttgaccgag ggcgagttgt cgcggttgcg
    18841 ggcaagtctg gtcaatgagg gcgtgctggc ggaaatggcg gcggaaatga atgtcggcga
    18901 cggtctgtac ttgggggcgg gcgagttgaa gagcggcggc ttcagacggc cttcgatact
    18961 ggcggacgcg atggaggcga tgtttgcggc ggtcagcttc gatgccgatt tcaacacggc
    19021 ggaaaaggtg gtgcgccatt tgtttgccga ccgcgtccgg tgtgccgatt tccaaaatca
    19081 ggcaaaagac ggcaaaactg ctttgcagga ggcgttgcag gcacgccgct ttgctttgcc
    19141 gaaataccgt atcgaagagc aaatcggcca tgccaacgac agtatgtttg tggtttcctg
    19201 cgatttgggc gaactgggtt tcgtgtgccg tgccaaaggg acgagccgca aggcggcgga
    19261 acaggaggcg gcgaaagagg ctttgaaatg gctggaagag aagctgccgc tgaagaagaa
    19321 aaagaaatga ggcggcgcgt gaatatgccg tctgaaatgt ggatatgaaa gcgaatatgg
    19381 atattgaaac cttccttgca ggggaacgcg ccgccggcgg ataccgttgc ggcttcgtgg
    19441 cgattgttgg tcgtccgaac gtgggcaaat caacgctgat gaaccatctc atcggtcaga
    19501 aaatcagcat caccagcaaa aaggcgcaga cgacgcgcaa ccgcgtaacg gggatttata
    19561 ccgacgatac cgcgcagttc gtgtttgtcg atacgcccgg ctttcaaacc gaccaccgca
    19621 acgcgctcaa cgacaggctg aatcaaaacg ttaccgaggc gcttggcggc gtggatgtgg
    19681 tggttttcgt cgtggaagcg atgcgcttta ccgatgccga ccgtgtcgtg ttgaaacaac
    19741 tgcccaagca cacgccggtc attttagtgg tcaacaaaat cgacaaggac aaggcgaaag
    19801 accgttacgc gctggaggcg tttgttgccc aagtgcgcgc cgaatttgaa tttgcggcgg
    19861 cggaggcggt cagtgcgaaa cacggattgc ggattgccaa cctgttggag ctgctcaagc
    19921 cgtatctgcc cgaaagcgtg ccgatgtatc ccgaagatat ggttacggac aaatcggcgc
    19981 gttttttagc gatggaaatc gtgcgtgaaa aattgttccg ctatttgggc gaggaattgc
    20041 cttatgcgat gaacgtcgaa gtggagcagt ttgaagagga agacggtttg aaccgcatct
    20101 atatcgccgt tttggtcgat aaggaaagcc aaaaggcgat tttaatcggc aaaggcgggg
    20161 agcgtttgaa gaaaatttcc accgaagcgc ggttggatat ggaaaaactg tttgatacca
    20221 aagtattttt gaaggtctgg gtcaaagtca aatccggttg ggcggacgac atccgcttcc
    20281 tgcgcgagct gggtttgtag tttttcttgc tgaactttac gcaaatgccg tccgaacagg
    20341 tttcagacgg cattttgttt caatcgggaa tatctttgtt aaaaacgggt tgatattatc
    20401 tgtgca
//
LOCUS       DAWHRA010000020        19790 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWHRA010000020 DAWHRA010000000
VERSION     DAWHRA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19790
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.020"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(203..278)
                     /locus_tag="WIB30_000549"
     tRNA            complement(203..278)
                     /locus_tag="WIB30_000549"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="WIB30_000550"
     CDS             complement(371..730)
                     /locus_tag="WIB30_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ3796669.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="WIB30_000551"
     CDS             complement(753..1397)
                     /locus_tag="WIB30_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ3796670.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1399..1935)
                     /locus_tag="WIB30_000552"
     CDS             complement(1399..1935)
                     /locus_tag="WIB30_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ3796671.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="WIB30_000553"
     CDS             complement(1947..3044)
                     /locus_tag="WIB30_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ3796672.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="WIB30_000554"
     CDS             complement(3047..4483)
                     /locus_tag="WIB30_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ3796673.1"
                     /translation="MENSLIIGLALAVLLVLLVMRAKQGKKTPKRKSQGVGNTQGQPP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEVEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WIB30_000555"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WIB30_000555"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ3796674.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="WIB30_000556"
     CDS             complement(5473..6120)
                     /locus_tag="WIB30_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ3796675.1"
                     /translation="MKTKFYNYQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTARCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WIB30_000557"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WIB30_000557"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ3796676.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFSRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            7495..8570
                     /locus_tag="WIB30_000558"
                     /pseudo
     CDS             7495..8570
                     /locus_tag="WIB30_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
     gene            complement(8777..10243)
                     /locus_tag="WIB30_000559"
     CDS             complement(8777..10243)
                     /locus_tag="WIB30_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ3796677.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10441..11364)
                     /gene="speB"
                     /locus_tag="WIB30_000560"
     CDS             complement(10441..11364)
                     /gene="speB"
                     /locus_tag="WIB30_000560"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ3796678.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11463..13355)
                     /gene="speA"
                     /locus_tag="WIB30_000561"
     CDS             complement(11463..13355)
                     /gene="speA"
                     /locus_tag="WIB30_000561"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ3796679.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPAQHNQTASLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13522..13806)
                     /locus_tag="WIB30_000562"
     CDS             complement(13522..13806)
                     /locus_tag="WIB30_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ3796680.1"
                     /translation="MKKLLLAAVVSLSAATAFADDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13957..15765)
                     /gene="aspS"
                     /locus_tag="WIB30_000563"
     CDS             complement(13957..15765)
                     /gene="aspS"
                     /locus_tag="WIB30_000563"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ3796681.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            complement(15823..16524)
                     /locus_tag="WIB30_000564"
     CDS             complement(15823..16524)
                     /locus_tag="WIB30_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ3796682.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16667..17815)
                     /locus_tag="WIB30_000565"
     CDS             complement(16667..17815)
                     /locus_tag="WIB30_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ3796683.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17975..18238)
                     /gene="rpsT"
                     /locus_tag="WIB30_000566"
     CDS             complement(17975..18238)
                     /gene="rpsT"
                     /locus_tag="WIB30_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ3796684.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18541..19683
                     /locus_tag="WIB30_000567"
     CDS             18541..19683
                     /locus_tag="WIB30_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3796685.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5091 a         5118 c         4593 g         4988 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gctgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcgctctac cgactgagct atcggggaac aagcgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caggtaagca gcttctcact gctgaccatt
     1261 tcgtccgcat ccgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaaggccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgctg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcctta accgcatcgt ctgccggaga cgaaccggtg ttgatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ctcatggtaa cccaggctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgta ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcacggacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcgactt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttcc gccaagacgc
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaggaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggcggctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcacca
     4441 gcaataccgc cagtgccaga ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg cgcactttgc ccgacggcat actgaacgcc gacaagtaat
     4801 cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatcta acggcgtatc aaagtctagt attaccttaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agtctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgctt tcaagcctgt tttgcaatgg gcgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacatcg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgcg ccattagatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgccg tcaatttccc gaacggtgca acgcgctgtc caatatcggt gctgattggc
     5581 aggcataaaa attttatcgc ccgatccgac caaggcattt gtccagcgaa tcaaatctgc
     5641 gcgcctatct tgcccgatca tcgcaaaaag tgcgataagt tcttgatgaa tttcgtcaaa
     5701 cggacgggcg ggaaattgtt ggtaatcttc aaaagatgct ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgggtgt tttccgtgag gttctccaat gtgcctttaa aaacggcgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcatagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgttc gccttgataa ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggcacg ccgcaaactg ccgcacttca tccattgtca tatctgccgt
     6181 caaagacaat cgtattctgg atgtaccttt tggtactgtc ggtggtctga tgggcaggca
     6241 ataataaccc tgcctttgca ggtattccgc tttggcaagg gtggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcgcttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc gaccaataaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gggcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacgggaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattca gcattttgca gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gctgcactat tgataagcgg tttttcatac gcaaacaccg tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgacccc aaactcggtt tgggtataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgcg tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcgggtctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaagtat tgcgcaatgg gcaaaaacac
     8161 agggtaaaac catcgcgccc gagaaagtcg atgctgtact caaagaccgc accggatcgg
     8221 ccgtctgcct gacctgcggc aaaaacggca agatgaagat gccgctcaaa tcgctggcgt
     8281 ggatacaggg ttcttcctca tacagccaag ctgaagtcat tgaacaaact gaggaaacca
     8341 actctgccga aatatccgag acacgcacgc ccgaagtgcc tgatgtacac acgcccgagg
     8401 cacaaccgca tttaaatacc caatccgacg gcacgccgac tgcctatacc gaaccggctg
     8461 ccgattcatc gccgcaagta gaaacacctg atcaggctgc ttccgagccg gttgatgtat
     8521 tattttcaat agatgtgata cggcagggaa atttgcgttt aggtaattga ataaccttct
     8581 gattatttaa tgcttatgtt tatcagaagt tgataggcgg tttggttttt gtttggcttt
     8641 tcctacaccg ccgcctgccg ctttctgcaa cattcaagcg cacaaatatg ccatctgaag
     8701 gctttagacg gcatatttca cgataatagt cgcccaatca aataaacaca gattggaaat
     8761 ttattttatt tttcccttac caatacccca gaactttcca ccaaatgctg ccgataacga
     8821 aaaaaatcag aaaattgact acgctcatga taaaacccgc cttccaccat tctcccattg
     8881 tggtgtagcc cgaaccgaaa atcacaggcg aagtaccggt cgcataatga gtgagggtca
     8941 tcataatgtt ggatgcggcc gccatcatca gcgcggtcgg catcgccggg gcattcagtg
     9001 aaacggcagc agcgaaaaat gcgccgaaca tagcggtaat atgtgcagta gtactggcaa
     9061 acatataatg cgcatacata taagcaagca cgaggattac gcccgcagcc gtgccgctaa
     9121 cgcccaaacc gccgacactt tccgccaaca ctccggagaa ccatttaatc agtccgagtt
     9181 tatttaaaaa tgcggccatc ataatcaatg cgccaaacca aataatcgta tcccacgcgc
     9241 ttttttcttt caaaacatcg tcccaagtca atacaccgga aagcaaaagc aggcttaatc
     9301 cgataaatgc ggtggcggtg gcgttgatac taaaagcgtg attgccggta ataagggcgg
     9361 gaacatctgc ccacaacagc agcaagatac cgaaaatgac cgccataatg atttcgtctg
     9421 ccgacatttt acccatctcc ctcagacggt ctttggcaaa ttgaacggca ttgggcgttt
     9481 ctttaatttc aggcggatac aaaaaatata aaatcaaagg cataacgaaa aaggcgataa
     9541 cgccgggaac agccattgcc cacgcccacg ccccccaaga aagacggaaa ctactgccta
     9601 aattttcggc aatcaagttg acgattaaag ggttgggggc agttgcagta ataaacatag
     9661 ccgacgaaat gggattggaa tgatagttga ccaaagccaa atatttaccc atcttgcctt
     9721 ctgtgccttt tgcgggattg gagccgtaac tgccggcaat cgactgcata atcggatgta
     9781 taatgccgcc gccgcgcgcg gtattggaag gggtaacggg agccagcagc agttcggaaa
     9841 gagcgagact gtaaccgatg cccagcgttt ttcttccaaa aacggcgata aacaaatatc
     9901 cgatacgcat ccccagccct gttttgagca aaccgcgcga aatcataact gcgatggcaa
     9961 tcagccaaat caacggattg gcgaacgcac tcaacgcatc gctcatcgcc gcgcccggtt
    10021 tgtcggcggt tacgccggtt actgcgacca acccgacggc aataatcgac agcgcgccca
    10081 acggcatggc cttgccgata atggcggcaa tcacaccgac aaacatggcc agcagcgtcc
    10141 aagcctgagg cttgaccccg tcgggtacgg gcagtgccaa aaccagggcg cacaatactg
    10201 cggcaatggc gaggggtatc ggtttgaaac ccaatttcat catattgacc tccgtaaaaa
    10261 agaccgtccc gaaaaatcgg aaaaataata tttaactaat tgttttataa gatatattct
    10321 gatatttcac cgtctttccg atatgcggct ccgggcaact tttgtttcag tatttgaatt
    10381 ttcattagac tgaatacgcc gtttgaacgg cacggcgaaa acccggggga tgccggacgt
    10441 tcagtccttt ttcgcacctt gaaggtaaag catttccaag gcaattgtgg cgccggccaa
    10501 agcggtaatg tcggattggt cgtaagaggg ggcaacttct acaacatcca taccgacgat
    10561 gtcgagatcc gtcagcccac gtaggatttt taatgccctg tcgctgctca agccgccgca
    10621 tacgggcgta ccggtcccgg gggcgaacga cgggtcgagg cagtctatgt cgaaagtcag
    10681 gtaaacgggc atattgccga cggtttcttt gattttacgg acggtctctt caacactgtc
    10741 ttcattgact ttaggggcgg acaacacagt aaaaggcaat tttttactgt gttcggtgcg
    10801 tatgccgatt tgtacggaac gggacgggtc gatgaggcct tccttggggg cggtatagaa
    10861 catcgtaccg tggtcgtatt cgctgccgtt gtcgtaggtg tcggtgtgcg cgtcaaaatg
    10921 aatcagtgcg agtttgccga aatagcgggc gtgggcgcgc aacaacggta gggtaatgaa
    10981 atggtcgccg cccaaactca aacagcgttt gccggaagaa agtaatttgc cggcgtgcgc
    11041 ttccattttt tcgacaaaat ccctgctgtc gccaaaagaa aaaaccaagt cgccgcaatc
    11101 aataatgttc aggcgttcgc gcacatcaaa tgtccatgga aacctgcggt gctcccaagc
    11161 gaggttgacg gaggcgcgcc ggatggcttc aggaccgaaa cgcgcgccgg aacgccctga
    11221 aaccgccata tcataaggca cgccggtaat aacccaatcg gcatgacttt catacggcat
    11281 aaaattaagc ggcaggcgca aaaacccgaa attattggaa acgagggagt tgtcggtttg
    11341 tcctgccagt gtgctgtatt gcatcgtaat gattccttgt aattggtttc aatcggtcgt
    11401 gatgattggt gtttgagtaa gaaaatcggg cttcagacga catatccgat gccttgatgc
    11461 gtctattcgt cttccaaata ggtataacca ttaagccccg cttcgagttc ttttaagaaa
    11521 gacatagcct gcgaggcagg aaggtctgaa tgttcgattt gttcgcgata gcgtttcatc
    11581 agctctttcg gatcttgata aacgtattcg agcatatcgg caacggtgtt tccttcatcg
    11641 taatcgatga cggtaaattg tccgtcttcc cctacaacaa catcggcagt ggcagtgtcg
    11701 ccgaaaagat tgtgcatatt gccgagtatt tcctgatatg ctcccaccat aaaaaagcct
    11761 aaaagcggcg gctcttcttc gggataatca ggcataggca tcgtaccggc gatgccgtct
    11821 ccgtcgatgt agtggtcaat cgtaccgtct gaatcgcagg taatgtccaa caacacggcg
    11881 cggcgcgcaa tcggttcatt caaaccggta atgggacaaa cagggaaaag ttgatctatg
    11941 ccccaagcat cgggcaaaga ttggaagagt gagaaattga catacagctt atcggcaaaa
    12001 cgttcttgca attcgtcaat aatggttcgg tgagaccggt gtttttcatt aaacaattcg
    12061 ccgacttcat gacagatatt taaatacagt tgctccgccc acgcacgttg cgccaaactc
    12121 aacagcccga cattatactg attatgcaca tcagcaagat caaactgccc ttcgtgtatc
    12181 cagctgcgta aggaacgttt ttcccgcgag gcggaaatat ccgtccaagt ttcccacata
    12241 ctgtgcaaca cacgcggtgc ttcgggcgat ggcgcatcca gccggcgcgg tttgtaacgt
    12301 tcaacgccta taacattagc aaccaaaacg gcgtgatgtg cggtaatgcc gcgcccgctc
    12361 tcggtgatga ttgtcggatg cggcagcccg tgttcgagac aagcctgact gatgccccat
    12421 acgactgtgg cggcatattc gttgaggctg taattaacgg aacaatccga ttgtgtgcgg
    12481 tttccttcgt aatccacgcc aagcccgccg cctacatcaa aacagcggat atttaccccc
    12541 agtttgtgca actcaacata aaaccgagcc gattcgtgta cacctgtggc aacatcacgg
    12601 atgttcccaa gctgcgagcc caaatggaaa tgcaaaagct gcaggcaatc cagcctgttt
    12661 ttttgtttca aaatatcgac cagttgcaaa acttgggaag ccgacaagcc gaattttgat
    12721 ttttccccac ccgaagactg ccattttccc gaaccttggg aagccagtct ggcgcgcaca
    12781 cccaaacggg gcttgatgcc gagtttttcc gcctcttcca ataccatttg tatttcggac
    12841 agcttctcaa tcaccaaata aacctgatgc cccagttttt cgcccatcaa ggcgaaacgg
    12901 atatattcac ggtctttata gccgttgcag acgattaatg tttgccgggt gccggcgtgt
    12961 gccaaaaccg ccatcagttc ggctttagaa ccagcttcca aaccatgcgg ttgtccgctt
    13021 gacataagcg attcgatgac gcggcggtgt tggttgacct tgatagggta aaccaaacaa
    13081 taaccgccct tatagccgca ctcttcccgt gccgtctgaa aggcgcggtt aatgtcgcgg
    13141 aggcggtgtt cgaggatttg cggaaaacaa aacaaaacag gcaggcgcgc ctgatgtttt
    13201 tgttgcacgg cttcagtcag tttttgcagt gaagcagttt gattgtgttg cgccggattg
    13261 gggcggacga tgatttcgcc ggaatcgtca acatcataat aacctatgcc ccaatgatta
    13321 atgttgcaca cttcacggat ggtaaggata ggcataataa acctgctccg tctgtcgtgt
    13381 tgaaaaggaa tgattataac aaatcagcgt gaaatgtcat ttttttaata agaaaagcct
    13441 gcctcatacc tgatgaggaa caggcaaaat gccgtctgaa cgcttcagac ggcattttgg
    13501 ttcatctttc catcaaagga gtcagcgatc gagctgctct ttgatgattt tcaggtcggg
    13561 gtaagacaac acgatgtcgt attcgcggcc gtctttatag gcttccactt ccaaaacagg
    13621 tttgccccaa tggtcgtcgg catcgacatc gtgaacctga taaccgcgct gttccaacat
    13681 ttttacggct tttgtgcggt tttgttcaaa atggggatca ccgtaaatct gacgctcggc
    13741 agaatcgtcg gcaaatgcgg ttgcggcact cagggaaaca acggcagcca ataacagttt
    13801 tttcattttc agtcctttct taatcggttg attgaataag atgtgttttt caatgctgcc
    13861 attaaaacac agcaaaatta ggtttgaatt agcaggaagt taagaagcgt aaatgccgtc
    13921 tgaaaaaaac agagccgtca aacggctccg ttttccttat gcttctttgg tttcagccac
    13981 cttttgacgc aggcgcaagc tcaattcgcg caactgcttg tcgtccacgc tgttgggcgc
    14041 gttggtcagc aggcattggg cacgttgtgt tttcgggaag gcaatcacgt cgcggataga
    14101 ttcggcgccg gtcatcaggg taaccaaacg gtcgaggccg aatgcaaggc cgccgtgcgg
    14161 aggtgcgccg aatttcaggt tgtccaagag gaagccgaat ttctcttgtt gctcttcagg
    14221 gctgattttc agcgcggcaa acactttctc ttgtacgtct gcacggtgga tacggataga
    14281 gccgccgccg atttcccagc cgttcaatac catgtcgtag gcgcgtgcca aacaatttgc
    14341 cgggtcggaa accatcaggt cttcatgacc ttcttttggc gcagtaaacg gatggtgtac
    14401 ggcaacgtag cggtcggctt cttcgtcgta ttcaaacatc gggaagtcga caacccacaa
    14461 aggtttccat tcgtctgtga agtagccgtt ttccgcgccg tgttctaaac cgactttaat
    14521 acgcaatgcg ccgatggctt cgttcacgac tttggttttg tctgcaccga agaagataat
    14581 atcgccgttt tgtgcgccgg tacgctcgat gatttctttc agggcattct cagacaggaa
    14641 ttttacgatt ggagattgca ggccgctgtc ttcgccgttg gaaaggttgc ccacatcgtt
    14701 tactttgata tatgccagac ctttcgcacc gtagatgccg acaaatttag tgtattcgtc
    14761 gatttctttg cggctgaatt tcgcaccgtt cggcacacgc agggcaacca cgcggccgcc
    14821 tttcatgtcg gctgcgccac ggaagacttt gaattcttcc gttttcatca ggtcggtcaa
    14881 ctcggtgaat ttcaagttga tacgcatgtc cggtttgtca gagccgtagt agaacatggc
    14941 ttcagagtaa ggcatgcgtg ggaagtcgcc caagtccacg cccagcgcgt ctttgaagac
    15001 ttgtttcgcc ataccttcgg tgatgtccat gatttcatcc tcgtttaaga acgaggtttc
    15061 caagtcgatt tgcgtgaatt caggctgacg gtcggcacgc aagtcttcgt cgcggaagca
    15121 cttggtgatt tggtagtaac ggtcgaaacc cgccaccatc aacagttgtt tgaataattg
    15181 cggcgattgc ggtagcgcga aaaactcgcc cggatgaacg cggctcggca cgaggtagtc
    15241 gcgcgcgcct tcaggcgtgg agcgggtcag catcggggtt tcaatgtcga tgaaaccttg
    15301 cgcgtctaga taacggcgaa cgcccatagc gacttggtaa cgcaggcgca ggttgcgctg
    15361 catcacaggg cggcgcaggt cgataacgcg gttggtcagg cgaacgtttt cgctgatgtt
    15421 ttcatcgtcg atttggaacg gaggcgtagc ggcagcgttc aacacttcga tttctttggc
    15481 aagaatctcg attttaccgg aaatcatttt gtcgttggtc gtgccttcgg gacggttgcg
    15541 tacgcggccg gtaatgctca aaacgtattc gttgcgggag gaatcggcag cggcaaacgc
    15601 ttcgggcgtg tcgggatcga tcacgacttg gacgatgcct tcgcggtcgc gcaggtcgat
    15661 aaaaatcaca ccgccatggt cgcgtcgacg gtgtacccag cctttgacgg taacggtttg
    15721 gtctaagtat tgctcgctaa tcaggccgca atagttggta cgcataaaat caccttttat
    15781 tgatttaaac tgaaaacaga aaatgccgtc tgaacggcgg ctttattgtt gttcgggcaa
    15841 atccgccttt tcagacggca taggtcctgc caatgttttg acgggcaggt cgtcagggat
    15901 gaccataccc agcgaaatca catatttcaa cgcttcgtcc acgctcatat cgagttcgcg
    15961 cacatcactt ttctttacca taatatagta accgccggtc ggattcggcg tggtcggaac
    16021 atacacggaa agataatcgc cgtccttcgg caatgcggcc ttaaccgcat tcgacacctg
    16081 accggacacg aatgcgattg tccaaatacc cgattgggga aacggcacga gtactggtgt
    16141 tttaaacgaa cggctgctgt cggacagcaa cgattcggat acttttttca cactcgaata
    16201 gatggacttc acaaccggaa tccgccccaa caggctgtcc cacgcggcaa gaatctgccg
    16261 gcccaatacg tttgcggcaa acaatccggt tacaaacaat acggcaatgg caacaataac
    16321 gcccagcccc gggatattaa accccaaaac atattgcggc cgccattgct tcggcagcag
    16381 gttgacaagc tggtcggacg cggaaacgat ataggaaacc acccaaaccg ttaccgcaat
    16441 cggcagccag accaaaatgc ccgtaatcag atattttttt aacgccttgg cagctttgcc
    16501 gccttcggcc gcaggttccg tcatcttgct tgattccgac aaagtccgta caaaccgcac
    16561 attatacgcg tttgcccgga ttcaaacgaa atttttatcc cgccccgcca aaccgccggc
    16621 ggcttcagac ggcacggcaa cttgatatat cgtctgaaca cgcggttcag atgccgtcca
    16681 agtcgttgaa catcaacccg ataccgatac cgttctgctt gtggttgtag tcgatcaggc
    16741 tctcgccgta accgtggaat ccgcgtacca cgcctttgag tttgccctta atcggaaacg
    16801 tgtaggcggc ttcaatcgcg ccgtagcccg ttttggggtt gtagcgcaat acggaataca
    16861 cattctgcct gtcgttcagg cggtactgca gcttcacgtc gccatacccc atatagtcgg
    16921 caatatcggg attgtcgttt ttatcgccgc tctgatcgaa cgcacgcacc cacacgcgcg
    16981 gaatcaccgt caatttgccc cattccatgc ctgccatggc gtaaatcctg ttccacgaac
    17041 gcgattcggg acggctctgt ccgttggact ggtggacaaa acccgcaccg agcatacgca
    17101 gcctgccgcc gaacggcaaa tccgccttca caggctgggt caggaaaatt tcaggtttgt
    17161 aatccgtatt gcggaacggc gcggatttcc tgccttggtt gtaaatctgc caatcggatc
    17221 tttgggtgta gccgaaccac agatccgcgc gggttttaaa caaatcttcg gcaattttgc
    17281 ttttgaacga aacctgcaat ttggtttccg cacgtttctg ctgtccgaat ttttcctgta
    17341 cagtcgtacc gcgcgtcggc gaacccgggg catagttggg cgaattgttg taccagagcg
    17401 gcataaggta catcggattg tgttcgcgta cgcccaacag cccgcgcaaa tcgtttttgt
    17461 ccaagtcgta catcaggctc aaaggcgtat agatgtcggc ggtttcgccc gcactgtcgg
    17521 caggaagcgc atccccgcct ttttcaacaa caatgaccgc ctcgccctta tccaggctgc
    17581 tgcggacggt ttccgtcaga ttgagtacgg ctttcgactc ctgcccttcc tgccctgccg
    17641 aagacggaag ctgtgccgca aaaatcctgt cgtaacacgc caaacgcgta acattgtccg
    17701 tcaaagcggc gcattgcagc gcggtctctc caaaagcgga tgccatcggc aacagtcctg
    17761 tcaaaagaat atagcgcata ttccgtgtat tcatctccgc ccccattgtc ggcatattgg
    17821 ttttcaaacg gcattttata gcggattcgg ataaaaaccg caccctttcc gccatttcgg
    17881 gattttgccc ccgcaataca gaaaaccccg aaaccgtcag gcttcagggt tttccgctta
    17941 tcgcgtatca accgccttgg cggttttgca aaaatcaagc cagtgctttt acttttgcag
    18001 acagacggct tttgtggcga gccgctttgt ttttgtggaa cacgcccttg tcggcgatgc
    18061 ggtcgatgac tttgacagac tcttggtaaa ccgcttgtgc ggcagcttta tcgcctgctt
    18121 cgactgcttt caatactttt ttcactgcgg tgcggaatgc ggtacgcagg ctagcgttgt
    18181 gggcgcgttg tttgacggac tggcgggcac gtttgcgtgc ttgtgcgctg tttgccatat
    18241 tggaatctcc tggaagaaaa ataaattctg taaacgcgca attttaaagg gggtttacat
    18301 tatatgcaag ccttttctct ctatccgccc gtcggacacc ctgacagcgc aaaaaaacgg
    18361 caaaatcgtg cggacgtttt ccccctcttt tgaaaacagg gtaatttttt agggaaatca
    18421 gtccaatatc actttgagtt tgcacaaatt tactttacaa tgcccaaacc ttgcgccggg
    18481 gtgtttatgg ggcgcggggt tttctttttt tccaacctct ttcatcagga atctgcccgt
    18541 atgaaaaaat ccgtattagc cgttttggcc gcgctgtctc tggcagcctg cggcggcagc
    18601 gaaaaaaacg ccgtgcaacc tcaagcagac gctgcgtctg ctgccaatgc cgaagccgct
    18661 gccaccgata ctctgaacat ctacaactgg tcgaactatg ttgacgagag tacggtcgaa
    18721 gactttaaaa aagccaacaa tctgaagctg acttacgact tgtacgaaaa caatgaaacg
    18781 ctggaagcca aaatgctgac cggcaaatcc ggttacgatt tggttgtgcc gggcatcgcc
    18841 ttcctgccgc gccaaatcga ggcgggcgcg tatcaaaaag tcaacaaaga cctgattccc
    18901 aactataaaa acatcgatcc cgaactcttg aaaatgctgg aaaccgccga cccgggcaac
    18961 cagtatgccg tcccctattt ctccggcgtg aacacgattg cgattacggc gaagggcaaa
    19021 gagcttttgg gcggcaagct gcccgaaaac ggctgggatt tgctgttcaa acccgaatac
    19081 acccacaagc tgaaatcctg cggcatcgcc ctgtgggaca ccccgagcga aatgttcccg
    19141 attttgctga actacttggg caaagacccc aaaggttcga atcctgaaga tttgaaggcg
    19201 gcggcggaag tattgaagtc tatccgtccg gatgtcaaac gtttcagccc gtccatcatc
    19261 gacgagctgg cgcgcggcga catctgcctg gcggcaggca acggcggcga tttgaacttg
    19321 gcgaaagcac gttccgaaga agtgaaaaac aacgtcggca tcgaagtgct gaatccgaaa
    19381 ggcatgggct tctggattga gtcttggctg attcccgccg atgcgaaaaa cgtcgccaat
    19441 gcccacaaat acatcaacta cacgctcgac cccgaaatcg cggcgaaaaa cggcatcgcc
    19501 gtaacctttg cccccgccag caaaccggcg cgcgaaaaaa tgcctgccga gctggtaaac
    19561 acccgttcca tcttcccgaa cgagcaggat atgaaagacg gtttcgtgat gccgcaaatg
    19621 agcacggatg cgaaaaaact gtctgtcagc ctgtggcaga aaatcaaagt cggcaccaac
    19681 tgatttgaag cattaaaaat gccgtccgaa catcgttcgg acggcatttt atattggatt
    19741 gaaatagaaa tatttatata gtggattaac aaaaatcagg acaaggcgac
//
LOCUS       DAWHRA010000021        19732 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWHRA010000021 DAWHRA010000000
VERSION     DAWHRA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.021"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..896
                     /gene="trxB"
                     /locus_tag="WIB30_000568"
     CDS             <1..896
                     /gene="trxB"
                     /locus_tag="WIB30_000568"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ3796686.1"
                     /translation="AVYAARANLNPVIITGIAQGGQLMTTTEVDNWPADADGVQGPEL
                     MARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEYTCDALIVATGASAKYLGLP
                     SEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEALYLANIAKTVTLIHRRSEF
                     RAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNGALLKNNDGSEQQIAVSGIF
                     IAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNIEGVWAAGDVKDHTYRQAIT
                     SAASGCQAALDAERWLGSQNI"
     gene            1052..1420
                     /gene="rpsF"
                     /locus_tag="WIB30_000569"
     CDS             1052..1420
                     /gene="rpsF"
                     /locus_tag="WIB30_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ3796687.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            1421..1723
                     /gene="priB"
                     /locus_tag="WIB30_000570"
     CDS             1421..1723
                     /gene="priB"
                     /locus_tag="WIB30_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ3796688.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            1730..1960
                     /locus_tag="WIB30_000571"
     CDS             1730..1960
                     /locus_tag="WIB30_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ3796689.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            1977..2429
                     /locus_tag="WIB30_000572"
     CDS             1977..2429
                     /locus_tag="WIB30_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ3796690.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(2622..3428)
                     /locus_tag="WIB30_000573"
     CDS             complement(2622..3428)
                     /locus_tag="WIB30_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ3796691.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAIMVFTAFSSMLGQHKKRAVDWKTVFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTVVAFKTLHTGPQTASRPLPKLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(3429..4175)
                     /gene="pssA"
                     /locus_tag="WIB30_000574"
     CDS             complement(3429..4175)
                     /gene="pssA"
                     /locus_tag="WIB30_000574"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ3796692.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(4230..4652)
                     /locus_tag="WIB30_000575"
                     /pseudo
     CDS             complement(4230..4652)
                     /locus_tag="WIB30_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4758..5240
                     /locus_tag="WIB30_000576"
     CDS             4758..5240
                     /locus_tag="WIB30_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ3796693.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGVMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(5292..6503)
                     /locus_tag="WIB30_000577"
     CDS             complement(5292..6503)
                     /locus_tag="WIB30_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ3796694.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASITHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            6726..9164
                     /locus_tag="WIB30_000578"
     CDS             6726..9164
                     /locus_tag="WIB30_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ3796695.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSSTD
                     LNGSRKILAHKDHL"
     gene            9372..10682
                     /locus_tag="WIB30_000579"
     CDS             9372..10682
                     /locus_tag="WIB30_000579"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ3796696.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADPSRLQAPTSLAVESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            10778..11392
                     /gene="clpP"
                     /locus_tag="WIB30_000580"
     CDS             10778..11392
                     /gene="clpP"
                     /locus_tag="WIB30_000580"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ3796697.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCDRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLRL"
     gene            11416..11610
                     /locus_tag="WIB30_000581"
     CDS             11416..11610
                     /locus_tag="WIB30_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796698.1"
                     /translation="MPSETFRRHLFQAAFDMVSIPSSTGRKKDLEFFKGSPFPPVMDG
                     CRLKAKWGSSFCKGATLASS"
     gene            complement(11835..13100)
                     /gene="ispG"
                     /locus_tag="WIB30_000582"
                     /gene_synonym="gcpE"
     CDS             complement(11835..13100)
                     /gene="ispG"
                     /locus_tag="WIB30_000582"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ3796699.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSSLESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGLKRHQGKVIPIHSL"
     gene            complement(13116..13877)
                     /gene="pilW"
                     /locus_tag="WIB30_000583"
     CDS             complement(13116..13877)
                     /gene="pilW"
                     /locus_tag="WIB30_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ3796700.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(13880..14974)
                     /gene="rlmN"
                     /locus_tag="WIB30_000584"
     CDS             complement(13880..14974)
                     /gene="rlmN"
                     /locus_tag="WIB30_000584"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ3796701.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(15117..15542)
                     /gene="ndk"
                     /locus_tag="WIB30_000585"
     CDS             complement(15117..15542)
                     /gene="ndk"
                     /locus_tag="WIB30_000585"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ3796702.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(15601..16752)
                     /locus_tag="WIB30_000586"
     CDS             complement(15601..16752)
                     /locus_tag="WIB30_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ3796703.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLTEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            complement(17143..17970)
                     /gene="fghA"
                     /locus_tag="WIB30_000587"
     CDS             complement(17143..17970)
                     /gene="fghA"
                     /locus_tag="WIB30_000587"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ3796704.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDDAYDLGQSAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(17979..19088)
                     /locus_tag="WIB30_000588"
     CDS             complement(17979..19088)
                     /locus_tag="WIB30_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ3796705.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            19236..19643
                     /locus_tag="WIB30_000589"
     CDS             19236..19643
                     /locus_tag="WIB30_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ3796706.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
BASE COUNT         4963 a         4844 c         5137 g         4788 t
ORIGIN      
        1 ccgccgtcta tgccgcgcgc gccaatttaa accccgtcat tattacaggt atcgcgcaag
       61 gcgggcaact gatgaccacc accgaagtgg acaactggcc tgccgatgcc gacggcgtgc
      121 aagggccgga attgatggcg cggtttctcg cccacgccga acgtttcgga acggaaatca
      181 tttttgacca aatcaacgcc gtcgatctgc aaaaacgccc gtttacactc aaaggcgata
      241 tgggcgagta cacttgcgat gccctgattg tcgcaacagg cgcgtccgcc aaatacctcg
      301 gtttgccgag tgaggaagcg tttgcaggaa aaggcgtttc cgcttgtgcc acctgcgacg
      361 gtttcttcta taaaaaccaa gatgttgccg tagttggcgg cggcaatacg gcagttgaag
      421 aggcactcta ccttgccaat atcgccaaaa ccgttaccct gatccaccgc cgcagcgagt
      481 tccgtgccga aaaaatcatg atcgacaaac tgatgaaacg cgtggaagag ggcaaaatca
      541 tcctcaagct ggaaagcaac ctgcaagaag tactgggcga cgatcggggc gtaaacggcg
      601 cattattaaa aaacaacgac ggttctgagc aacaaattgc cgtcagcggc atttttatcg
      661 ccatcgggca caagccgaat accgatattt tcaaaggtca gttggaaatg gacgaagccg
      721 gttacctgaa aaccaaaggc ggcacggcgg acaatgtcgg cgcaaccaat atcgaaggcg
      781 tatgggcggc gggcgacgta aaagaccata cctaccgtca ggcgattacc agcgcggctt
      841 ccggctgtca ggcggctttg gacgcggaac gctggctggg cagccaaaac atttgaatgc
      901 cgtctgaaac cgaaattttc ggcggaatgc aaatttttcg caccaaatcc ttgattccgt
      961 cggcaaaccc caatataatg ccgcttcttt aaaccttgcc ttctgctttc gcatggcaga
     1021 aggctgtttt taccatccat aaggagataa catgcgtcat tacgagatcg tgtttatcgt
     1081 tcatcccgat caaagcgagc aagtacccgc tatggttgaa cgttacaaaa ccatgattgc
     1141 cgaagcaaac ggtaagattc accgtctgga agactggggt cgccgccagc tggcttaccc
     1201 gattaacaaa atccataaag cccattacgt tttgatgaac atcgaaacca ctcccgaagt
     1261 ggttgaagag ctggaaaccg cattccgctt caatgatgca gtattgcgtc atctgaccat
     1321 caaaaccaaa cacgccgtta ccgaagcatc ccctatgttg ggtggtgaaa aggctaagaa
     1381 cctgttgagc ggtgcgtctg aagaagcggt cgcccaataa ttgggattca ataatcttgt
     1441 ttcgcttgcc gcgttaattg aaaaggtttt ccctattcga tatacgcctg ccggaatccc
     1501 tgttttagat attattttaa agcacgaatc gtggcaggag gaaaacgggc agcaatgcct
     1561 tgtccaattg gaaattccgg cacggatttt aggcaggcag gcggaagagt ggcagtatcg
     1621 gcaaggtgta tatgttcacg tcgaaggttt tttagctcaa aaaagcagac gttcccttat
     1681 gccgatgctc aggatacaaa atattcaaga atataaaggt taaacgacaa tggctcgtca
     1741 atcattcaaa cgtagaaaat tctgccgttt cacggctgaa aaaatccaag aagtcgatta
     1801 caaacaagtt gatttgctga aagacttcat ctccgaaaac ggtaaaatca ttcctgcacg
     1861 catcacagga acgaaggcat tctaccaacg ccaattggct gttgccgtaa aacgcgcacg
     1921 cttcctggct ctgctgcctt acaccgacca acacaaataa ttttggagat tgaatcatgc
     1981 aaattattct gttagaaaaa atcggcggtc tgggcaactt gggcgacatc gtaaccgtta
     2041 aaaacggcta cgcccgcaac tttctaattc ccgcaggtaa ggcaaaacgt gcgaccgaag
     2101 cgaatatgaa agagtttgaa gcacgccgcg cagaactgga agccaaacag gctgaaattt
     2161 tggcagatgc ccgagtccgt caggaaaaat tggacggtca aaccgttacc gttgctcaaa
     2221 aagctggtgt ggacggtcgc ctgttcggtt ccgttaccaa tgccgacatt gctgctgcaa
     2281 tcgttgctgc cggcatcgaa gccgtgaaag caaatgtacg tctgccgaac ggtcctctga
     2341 aagccgttgg cgagtacgaa gtggaagtgg ctttgcacac tgatgccgtt gctaaaatta
     2401 ccgttgccgt cgttgccgca accgagtaat tgaaattatc agatgccgtc tgaaaccctg
     2461 ccgattgtcg gaatgtggtt tcagacggta tttgttttgt ttttcgtata ttctgcaaag
     2521 gtctcggtca ggttgtctgt ccttttgtcg gaacaggaga ctgggcagct ttgtcctgat
     2581 taattgcttg tgcaaacggt aaaaaggttt tttcgtgtgt tttaaagcag gttgtacagc
     2641 atttttccgg caatcaaaag caacataatg ccgaaggatt ttttgagttt ggcagaagaa
     2701 agtttgtggg cggttttgac accgagcggg gcaaaggcaa tggttgccgc gctgaggacg
     2761 gcgacggcgg gcaggtaaag gaagcccagc gacccttcgg gcaatcctgc aatattcagg
     2821 ccgttgagca gatacgatat tgcgccggag agtgcaatcg gccaggcaag gccggatgat
     2881 gtgccgatgg ctttatgggc ggggaagccg cagtggatta agaaggggac ggaaagtgaa
     2941 ccgccgccta tgccgaccca gctcgacatt gcgccgaaca gtgtggaaac cgcagtcagt
     3001 ccgggcagct tgggcagcgg gcgggatgcc gtctgagggc cggtatgcag tgttttgaat
     3061 gcgacgacag ttaaaaacag gatgaagaag atttgaagcc cgaatgcggg gatatatttt
     3121 gcggagagtg cgcccgcgaa tacgccgaat atcatacccg gcatcatcgc aaatacggtt
     3181 ttccagtcga ccgctcgttt tttgtgctgc cccaacatac tggaaaaggc ggtgaagacc
     3241 atgatggcga aggatgtgcc gacggcgagg tgttgcgcgt aaggatgttg tgccaaaccc
     3301 tgcaaatcaa gcacccataa aacgacaggg acaatcagcg tgccgccgcc tacgccgaac
     3361 aggccggcga taaaacctgc cgcactgcct acggcaagca ggattaagat aatgtcccaa
     3421 tgccacattt aatccgcctt tctgtatttt ttccagaatc ggcgtgccgc cataatgtag
     3481 ccggacaggc tgtatccgag aaagaacagg aagaggacga gcgacggttc ccaagtgacc
     3541 agaagcagca gtaagacggc aagcagcatt ccgacaaagg ggacttgtct gcggatgttg
     3601 atttctttaa aactccaaaa agggatttgg acaatcattg acaggccggc aaacagtgtg
     3661 atgcccaatg cccaccagtg gacggcgggg aatttttcga cgctgtggtt gacccaaatc
     3721 agcccgacaa tcagcgcggc ggcagtcgga ctgggcacgc cgataaacca gcgtttgtcc
     3781 accttgccga tgagcgtgtt gaacagggcg aggcgcaggg ctgcgcaggc gcagtagatg
     3841 aaggcgacgg aataaccgat tttgccgaac tgccaaagct gccatttgta ggcaataagc
     3901 gcgggggcaa cgccgaagct gaccatgtcg gcaaggctgt cgagctgctc cccgaacgcg
     3961 ctttggctgt tggtcagccg cgccacgcgc ccgtccatac cgtccaaaag catggaaatg
     4021 aatacggcaa tagcggcggt ttcataacgt ccgtgcatgg attgggtgat tgcgtaaaac
     4081 gcggaaaaca gcgcggcgat agtaaaggaa ttgggcagca ggtagatact gttttgacgc
     4141 agggagcgtt gcggggtgtt ggtcggggtt tccatagctg aagtgtctca cggacgtgga
     4201 gaatcgatag gggattataa cccaaaccat tatcgaacaa tacgcccttt aggatgaagc
     4261 cctcggaggc ggtttgtttt tctgatggcg gatgctttgc ggggcggttt gactctgcct
     4321 gccgccgggt gtgttgtttc ggatttttgc cggatggatg ttgttgccgg gcatttctgg
     4381 gcttgccgga aggtatcggg gggagggcat cggaatatgc ggcgcgccgt tcaaactggg
     4441 gcgacgtttc cctattttca gggggttgca ggtttcaaca aattgaaaga tattttttgc
     4501 atttccgacc gtatgctgat tttgaaacgg gataaattat aggacaaggt ttttcgcccg
     4561 ccgcaaaggt tggccgcaat gcagtctgaa aacaacggct tgccgctaag gcggcggtat
     4621 gcggatttgc gctgccgaaa ccggtttgcc atacccggcg gtgcaatctg cggggaggga
     4681 taagccgata aggcgggact tatcctgttt tttgtttata atgcaacaga atttggacga
     4741 accgtcggga atttatcatg atgaaaaaga tactggccgt atcggcacta tgcctgatga
     4801 ctgcggcggc acgggctgcc gatacttacg gctatctcgc cgtttggcag aatccgcaga
     4861 atgcggacga tgttttgcag gttaaaacta caaaagaaga ttcgacgaaa agcgaagcgt
     4921 ttgccgagtt ggaagccttt tgcaaaggtc aggacacgct tgcgggcatt gccgaagacg
     4981 agccgaccgg atgccggtcg gtcgtgtcgc tgaacaatac ctgtgtcgcg ctggcatacc
     5041 cgaaagcctt gggcgtgatg cgcgttgaaa acgccgtcgt gattacttct ccgcgtttta
     5101 cgagcgttca tcaggtcgca ctcaaccagt gcatcaaaaa atacggcgta cagggacaat
     5161 gcggcttgga aacagtgtat tgcacatctt cttcttatta cggcggaact gtgcgctctt
     5221 tgattcaaaa tctcaaataa aacggaaaat gccgtctgaa agatgttcag acggcatttc
     5281 tatatcgacg gtcaggattc tttcggatcg ggcagcaggc tgttcaacat aatggcaagt
     5341 acggcgcaca agcccacgcc ggcaaagctg aagctgccca atttgagcgt catgccgccg
     5401 atgcccgtgg tcagtaccga gctgacgatg accaggtttt tcggcagcat caaatcgact
     5461 ttggcatcaa tcagcgtttt cacgcccaaa gaagcaatcg tgccgaacag cagcagcata
     5521 atgccgccca ttactggcat cggaatggaa gccaaaaacg cattgaattt gccgaaaaac
     5581 gccatgcaga cggcaaaaac cgccgcccaa gtcatgatga cggggttgct gtttttggta
     5641 atcatcaccg cacccgttac ttcgccgtag gtcgtaaccg gcgggccgcc gatcagaccc
     5701 gcaacgcata cgcccaaacc gtcgcctgca agggttttgt ccaagcccgg gtctttcgta
     5761 tagtctttcc ccgtcacatt gccgattgcc atgatgccgc cgatgtgttc gatggcgggg
     5821 gcgacggcaa cgggcagcat aaacagtgca gcctgccagt tgatctgagg cgtttcaaaa
     5881 tggggaacgg cgaaccaggg cgcgtgtgta atgcttgccg tgtccaccag tcccatcagc
     5941 agtgccaaaa cataacccga agcgacaccg atcaagatgg gaatcagctt catcatcctg
     6001 ctgccgaaaa ccgatacgat ggcggtaacg gcaaaggtaa agccggaaag aatcagcgaa
     6061 tcggtatagt cgatgacctg tttgccgtcc gcctgaccca ttgccatgct gcttgccgcc
     6121 acggcaacag acagaccgat gaccatgatg acggggccga tgacgaccgg aggcagcagt
     6181 ttgtgtaccg ctgccagtcc gcgccaacgg atcagcgcgg caaacacaaa atacataaag
     6241 ccggcggcaa acagtccgaa catggtggaa ggcagccccc attcgccgac ggagtagata
     6301 atcggtgcga taaaggcaaa cgaagaacca agaaaaatcg gcactttgcg tttggttgtg
     6361 atttggaaca gcagcgttcc caagcctgcg cccaaaagcg caagagccgg attcagaccg
     6421 gtcagcaggg gaaccagcac cattgcgccg aatgccacaa ataaaatctg tgcaccggaa
     6481 acggcaagtt tcagttggtt cataccatct tctttcaatc ggttcaaacg gcacggatta
     6541 tactggtttg ccgagggtct gcgtaaaaat acatacgtcg ggcaacagat atgccgtctg
     6601 aaaggcaatg tctgtctgtt gcaggatgaa agcaggagaa atggagtata atccgacact
     6661 ttccatattc aatccgcaag ccgtccggct tccttatttc agacggcatt ttccggtaat
     6721 acggcatgac agaaaaatcc cataaaaaaa cagcaaaagg cagggcggga agcccgtccc
     6781 caacgtccgc acgcaacaag aaagccgaca acggcgcacg gggcaataaa gtttccgagc
     6841 ggctcaaggc ggtcaaagag ttgcagaaaa ccgaaaccaa aaaggcgcgt cccgaacatg
     6901 tcgtcaacct tatcggcgac gcactgtggc tgatgggttt ggcggcaacc ctgtatttgg
     6961 cgatttccct gatcagtttc gatatgggcg atccgtcttg gtcgcacagt tcgccggttg
     7021 tggaagatgt cgccaattgg ggcggactgt tcggcgcgta tgttgccgat gtcggctatt
     7081 atcttttcgg ctggtcgttc tggtggtgga tagcggctgc ctgcgtcgtg ctgtataaaa
     7141 atttccgcct gcacgcaaaa cagacggaaa acgaggcata caaccacaaa atcgctgccg
     7201 ccgcgctgtt tgtcctgacg gtcttcagcc ccgtcttgga gtattttgtg ctgggcggaa
     7261 aatatgccga ctccctgcct gtcggagcag gcggtatggt cggcatacgc gtcggcgcag
     7321 tgtttgcgtg gctgctgggg aaatcgggca gcctgctgat tatcttggtt gttctgctgt
     7381 tgtcgttgtc cctgctggtg cagatttcat ggctggaatt tttgaacggt gcgggcaggg
     7441 cggttcaaaa ccgcctgagt gccttatccg gcaaggtcat ggctttagga aaacgccggc
     7501 cgaataccaa aacagacggt gtcgataccc aaaatacacg gcgcatggta aaagaagcca
     7561 agaatattac ggccaaaccc gttgccttgc ccgaaggcag cagcagcaac cgcaaatccg
     7621 tcgcggtttc cgtcgcgccg ccgcccaaaa ttcaggtttc tctgtttgaa gatgacgaac
     7681 ctcggcaggc gggcgaatac cacaagccta cattgaacct attgcggatt cctgacagcg
     7741 aacccgtcag catcaatccc gccgaattgg agcgcactgc cgaactgatc gaatccaaac
     7801 tggcagaatt cggcatcggc gtacaagtcg tatccgccac atccggcccc gtcatcacgc
     7861 gctacgaaat cgaacccgcg caaggtgtta aaggcagcca aattgttgcc ttgtcgaaag
     7921 atttggcacg ctctatgtcg ctgcagtccg tgcgtatcgt cgaaaccatc gcaggtaaaa
     7981 acacgatggg catcgagttg cccaacgaca aacgccaaga cgtgatgttg agtgaaatct
     8041 tgtcctcgcc cgtgtttgcc gaagccaaat ccaagctgac cgtcgcgctg ggcaaagaca
     8101 ttgccggtac ccccgttgtc ggcgacttgg cgaaaatgcc gcaccttttg gtcgccggta
     8161 tgactggttc gggcaagtcc gtcggcgtga acggcatgat tatgtctatg cttttcaaag
     8221 ctacgcccga cgaagtccgc ttcattatga tagacccgaa aatgctcgag ttgagcattt
     8281 acgacggtat tccgcacctg ctctgtcccg tcgtgaccga tatgcgcgaa gcagggcagg
     8341 cgttgaactg gtgcgtcgcc gaaatggaaa aacgctaccg cctgctttcc catgccggtg
     8401 tgcgtaattt ggagggcttc aaccaaaaag tcgaagccgc aaaagcggca ggcaagccgc
     8461 tgctcaatcc gttcagcctg aaccccgacg agcccgagcc gctggaaaaa ctgccgttga
     8521 ttgtggtcgt tatcgacgaa cttgccgacc tgatgatgac cgaacgcaaa gccgtcgagc
     8581 agcaaatcgc ccgtctcgcc caaaaagcgc gcgccgccgg tatccatatg attgtcgcca
     8641 cccaacgtcc cagtgtcgat gtcgttaccg gcctgattaa agccaacatc ccgacgcgta
     8701 tggcgtttac cgtgcaaagc aaaatcgaca gccgtaccat cctcgaccaa atgggcgcgg
     8761 acgaactgct caaatatggc gattcgctgt tcctccagcc cggcagtgcc gaaccgactc
     8821 gcctgcaagg cgcgtttgtt tcagacgacg aagtacatca agtcgtcaac tacgtcaaat
     8881 cgcaagcccc agccgactat attgaaggtc tgctcagcgg cgaggccgcg ctggaaactg
     8941 ccaatatcgt taatccgaat gcagacagcg acgaattgtt cgatcaggca gtcgcctatg
     9001 ttttggaaag caaaaaaacc tccatttcgt ctttgcagcg gcagctgcgc atcggctata
     9061 accgcgcggc aaacctgatg gaggcactgg aaaatgcggg tgtcgtttct tccaccgacc
     9121 tcaacggcag ccgtaaaatt ttggcgcaca aggaccattt gtagcccgta ttgcaaaatg
     9181 ccgtctgaac ggcggaattg gcgtttcaga cggcatatta tgtttcaggc gaaacattgt
     9241 gatatacttg ccagctaaaa tttccccttt gcggcaatgc ggttcaaata tcgtaccgtt
     9301 gcgctgtttg cttccccatg tagggaagaa agtttatcat tttatcaaca caacaaattt
     9361 aagggcttat gatgagcgta actgttgaaa ctttagaaaa tctggaacgc aaagtagtgt
     9421 tgtccctgcc ttggtccgaa atcaacgcag aaaccgataa aaaactgaaa caaacccaac
     9481 gccgtgcaaa aatcgacggt ttccgtccgg gtaaagcacc tttaaaaatg attgcccaaa
     9541 tgtacggtgc gagcgcacaa aacgacgtga tcaacgagct ggtgcaacgc cgcttctacg
     9601 atgttgccgt tgcccaagag ttgaaagtgg caggcttccc ccgttttgaa ggcgttgaag
     9661 aacaagacga taaagagtct ttcaaagttg ccgccatttt tgaagtgttc cccgaagtcg
     9721 ttatcggcga tttgtctgca caagaagttg aaaaagtaac cgcttccgtc ggtgatgccg
     9781 aagtggacca aaccgtagaa atcctgcgca aacaacgcac ccgcttcaac catgtcgaac
     9841 gcgaagcccg aaacggcgac cgcgtcatca ttgactttga aggcaaaatc gacggcgaac
     9901 cttttgccgg cggcgcatcc aaaaactacg ccttcgtatt gggcgcaagt caaatgctgc
     9961 ctgaatttga agccggcgta gtcggcatga aggctggcga aagtaaagac gttaccgtca
    10021 atttccctga agactaccac ggtaaagacg ttgccggtaa aactgccgtg ttcaccatta
    10081 cgctgaacaa cgtttccgaa gcgactctgc ctgaagtcga tgcagatttt gcaaaagcct
    10141 tgggtattgc ggatggcgac gttgccaaaa tgcgcgaaga agtgcagaaa aacgtaagcc
    10201 gcgaagtgga acgccgcgta aacgaacaaa ccaaagaatc cgtaatgaac gcgctgctca
    10261 aagccgtaga gctgaaagca cctgttgctt tggtcaatga agaagccgca cgcttggcaa
    10321 acgaaatgaa acaaaatttt gttaaccaag gtatggctga tgctgccaac ttggatctgc
    10381 ctttggatat gttcaaagaa caagccgaac gccgcgtatc tttaggtctg attttagcca
    10441 aactggttga cgaaaacaaa ctggaaccga ctgaagagca aatcaaagcc gttgttgcca
    10501 actttgcaga aagctacgaa gatcctcaag aagtgattga ctggtactac gcagatcctt
    10561 cccgcctgca agccccgact tctttggcgg tagaaagcaa cgtcgttgat ttcgttttgg
    10621 gcaaagccaa agtaaatgaa aaagctttgt cttttgacga agtgatgggc gcgcaagcct
    10681 gattatcctg aaaatgccgt ctgaaggcag tttttgaaag caccgaagcc gcttttaaag
    10741 ctcgctttgg tgcttttcca tcatgaaagg agacgaaatg tcttttgata actatcttgt
    10801 ccctaccgtt atcgagcaga gcggtcgcgg tgagcgtgca ttcgatatct attcccggct
    10861 tttgaaagag cgcatcgtat tcttggtcgg accggtaacc gacgagtccg ccaatctggt
    10921 ggttgcccaa ctgttgtttt tggaaagtga gaatccggat aaggatattt tcttctatat
    10981 taactcgccg ggcggttcgg taacggccgg tatgtcgatt tacgacacca tgaatttcat
    11041 caagcccgat gtatcgactt tgtgcttggg gcaggcggca agtatgggcg cgttcttatt
    11101 gtcggcaggc gagaaaggca aacgttttgc cctacccaac agccggatta tgattcacca
    11161 gcctttaatc agcggcggtc tgggcggtca ggcatccgac attgaaattc acgcacgcga
    11221 acttttaaaa atcaaagaaa aactcaaccg cctgatggcg aaacattgcg accgcgattt
    11281 ggcagatttg gaacgcgaca ccgaccgtga taatttcatg tctgccgaag aagccaaaga
    11341 atatggtttg attgaccaga ttttggaaaa ccgcgcttct ttgcggcttt aataaaagaa
    11401 acctgagaga aaccgatgcc gtctgaaacg ttcagacggc atttgtttca agctgcgttt
    11461 gatatggtat cgataccgtc atccacggga cggaagaagg atttggagtt ttttaaagga
    11521 tccccgttcc cgcctgtgat ggacggatgc cgtctgaaag ccaaatgggg cagcagtttt
    11581 tgcaagggcg cgacactcgc atcctcctga atctgccggt acggttattg agaaaagaca
    11641 agggcaggaa gcggtatccc gccttcctta tttatacctg caattcatct ccgtatttgc
    11701 cgattgtatt ttcatattgc gcctccattc cttatcggtt taaaaaaatg ccttttccaa
    11761 tgggatgggt tttatatcaa tgaaaataaa cggtttttat ttaaaaatag ccaaggtagg
    11821 ctggcggttg gattttatag ggagtgtatc gggataacct tcccttgatg gcgtttgagt
    11881 ccgttctccc cataattggt tttgacatac tcttcaacaa tagccagaaa ttccgttgca
    11941 atgttgtcgc ctttcagcgt tactttacgt tcaccatcta cataaacagg ggcaacgggt
    12001 gtttctcccg taccgggcag gctgatgccg atgtcggcca atttgctttc tccgggccca
    12061 ttgacaacgc agcccattac ggcaacgttc agggattcaa ccccaggata aagggtacgc
    12121 catatagaca ttttttggcg caggtaattt tgaacatctt gtgccagctc ttgaaatacg
    12181 gtactggtgg tacgcccgca gccggggcag gcggtaacca tcggcgtaaa cgaacgcaat
    12241 cccatagtct gtaaaatctc ttgcccgacg acgacctcct gagtacgcgg gctgccaggt
    12301 tccggagtca gtgaaatgcg gatggtgtcg ccgattcctt cttgaagcaa gacggataat
    12361 gccgccgttg atgcgacaat gcctttgctg cccataccgg cttcggtcaa acccaaatgc
    12421 agcggatagg cgcaacggct gcccagttcg cggtaaacct gaatcaaatc ctgaaccgcg
    12481 ctgactttgc acgacaggat gattttgtct tcgggcagtc ccaatagaac ggctttttcg
    12541 gcagattcca aagaggagac aatcagtgct tccttcgtca cttcttcggg cggtttcggc
    12601 gcggaagaag cgaggttggc atccatcata cgtttggcga ggctttgatc caaagaaccc
    12661 cagtttacgc cgatgcggac ggctttatcg ttttcagcag cagtccgaat cataaaggca
    12721 aatttttcat cgccttttac gcctttgccg acattgccgg gattgatgcg gtatttggac
    12781 aatgctttgc cgcattcggg aaattccgcc aacaggcgtt cgccgttgaa gtggaaatcg
    12841 ccgataagcg gtgtggcata gcccatatcg tccaagcggc ggcggatttc ggcaactttg
    12901 gacgcggctt cggggctgtt gacggtaata cgcaccattt cggatccggc atcgctcaat
    12961 tccttaatct gcaatgcggt ggcttttgca tcggcagtgt cggtgttggt catagattgg
    13021 ataacgacgg gtgcttctga accgacggta atatgatcga tgcggacttg atgcgtcttg
    13081 cggcgttgga gtgtgttcat atggtttgaa tctgtttatt gaccggtgag gacggtttgc
    13141 aattcttccg agtaggggaa attcgcctgc aattgtgctt catattcgta tgccgcctgt
    13201 gcgttgccga gggctttggc aattttccag cctagcagca aatcatcggc ctgaaggact
    13261 tctaccctgc tttggtattt tttaaagtag taatcggcat cgcccaactg cccggccagc
    13321 attttggtgc gcgccagttc tttaaatgcg ggtgggaact gcggctgggc ggcgagggaa
    13381 cgtttcaaat aggcttccgc caatccgaat tgcccctgtt ttgcgctgca tatgccttta
    13441 ttcaggttgg caatataagg ggtcgggtag gtggggtcgg ccagggcttt gtcgaaatat
    13501 gccatagatt cggcagggcg gttgagcctg ccgcacagga accagccgta gttgttgttg
    13561 atttcggcac tgtcgggttt gatggagagg gcttgccgga aactttcctg cgccttgtcg
    13621 ttaactttca ggtattgata gatttcggca cggaccagcc aggcaagctc gtttttaggg
    13681 tctgatttca aggcgtcttc aatacttgcc gtcgcctgac ggtagtcctg accgcgcata
    13741 tattccattg ccaactgggt tttgatattg gaaacctgat tggctttttc tgcccgcgag
    13801 gggcggtagg aagtgctgca cgcgcccaag gcaaggacaa gtaataaaga gattcgtttg
    13861 gatggcttaa aaggcataat tacccctgtt gtccgattaa aatctgctgc catttttgtt
    13921 ggcggcgcgt tttatcctga acctgccccg ccaactgtcc gcaggcggca tcgatgtcgt
    13981 cgccgcgcgt ttttcgtacg gtaacgacaa atcctgcctg ctgcaaaata tcgcggaaca
    14041 cacggatgtt ctcattgctg gagcgttcgt atccggagtt tggaaaggga ttgaacggaa
    14101 tcagattgaa cttgcaggga acatctttga ccagttcgat cagttcgcgc gcatgttgcg
    14161 ccttatcgtt tattccgtcc aacatgacgt attcgaaagt gatgaaatcc ctgggtgctt
    14221 tgaccagata gcgttggcat gcagccatca attctttcaa gggatatttt ttgttcaacg
    14281 gtacgatttg gttgcggact tcgtcattgg aagcgtgaag ggaaaccgcc aaagccaccg
    14341 gcatgacatc gcgcaacctg tccatttggg gaaccatacc cgaagtggaa acggttacac
    14401 ggcggcggct caaaccgtag ccgtggtcgt ccagcataat gcttaaggcg gtaacgacat
    14461 tgtcgaagtt cgccatcggc tcgcccatac ccatcatcac tacattggaa attacgcgtt
    14521 cgtttttcgg tgtaacaccc atcgctttgt ttgcccacca caactgtccg atgatttcgg
    14581 cagcagtcag gttgcggttg aagccctgcc ggccggttga gcaaaatgta cattccaaag
    14641 cgcagccgac ttgcgaggaa atgcagagcg tgccgcgatc cgattcgggg atgaagacgg
    14701 tttccacccc gttccccgtc ccgacatcta gtagccattt tcgagtgccg tctgaagatt
    14761 tttgagacat catcagcttg ggaatttcga ttcctgcctg ttcgttcagt ttatggcgca
    14821 acgattttgc caaatcggtc atttcgtcaa aattttgcgc gccggattgg tgcatccaac
    14881 gcataacctg tttggcacgg aaaggttttt cgcccatatc ggcaaaatgt cgggtcagcc
    14941 cttgaaggtc gtagttgagc agattggttt tcatgtgttt gtgtttttct taaattaagg
    15001 cttcaaataa aaaattgcag ggcaggcaag tttgatttga agccttttgc gacagagttt
    15061 tcagacggca tggatatatt tatgccgtct gaagaggagt tgtgcggtgt attgtatcaa
    15121 cgggggcaga tttcggtttg gctgaaaaag taggcaattt ccaaagcggc attttccacg
    15181 ctgtcggaac cgtgtacggc attaatgctg accgaagtgg caaagtccgc gcgtatcgtg
    15241 ccttcggcgg cttcggaagg attagttgca cccatcagtt cgcggttttt caggacggcg
    15301 ttttcaccct ctaatacctg aatcataacc ggaccgccgg tcataaattc aaccaatccg
    15361 gcgtagaagg ggcggtcttt atgaaccgca taaaattctt gcgcctcttt gagggtaagc
    15421 tgcttcattt tggcggcaac gattttcaga ccgttctcct caaagcggct gtatattttg
    15481 ccgataacgt ttttgccgac ggcatcgggt ttgatgatgg agatggtacg ttcaatcgcc
    15541 atgctatatc cttattttta cttaagaaga atcaaatcgg tattctatca aaaaaataac
    15601 ttatccgcct gaagccttgg gagatagggt caggtgcggc tgttccaaat aaacttcaga
    15661 ctgcatttcg accatccggc tggcggtgcg ggtaaattct tcggcaaaat cgccttccgt
    15721 atagatatgg ttgacatcta ccgcgccggt ggcacacagt ttgacccgga aatcgtagag
    15781 tacgtcaatc agccaagtca gccgccgcgc ctccgccttt tcttgcggtg agagttgttc
    15841 caaacctgaa ataaaaacca tttcataatg ttcggtcaaa tacagatagt cggactgtga
    15901 gcgggggccg aagcacagtg cgcggaaatc aaaccatatg gcacggccgg actcggcttt
    15961 gtggggaatc tcccgaccgt ggatggtgct gatgccgggg ttcaaatcgg taatgcctgt
    16021 catttctttg aacagttttg ccagttttgc ctcattttct tcattggcag gcgtaaagaa
    16081 aatctcggcg gggcggaggg tacgcagtcg gtagtcttca ccgccgtcaa cgtttaagac
    16141 ggtcaggctg gactcgatga gcgcgattgt gggaagaaaa ctgctccggt tttgaccttg
    16201 cgggtagagt tcggaaggcg cgtagtttga agtcgccacc aaaacaacgc cctcgttaag
    16261 caggttttcc agcagacggc ctaaaatcat tgcatccgca atatcgctga catgaaattc
    16321 gtcaaaacat aatacgcggg tttctttggc aatctcggcg gcaacggatt tcaacgggtt
    16381 gctttcgctt ttcagggttt tcagcctctg gtggatttct gccataaagg catgaaagtg
    16441 gacgcggcgt ttgcggcggt aagggaggca gccgaaaaaa gcgtccatca gaaagctttt
    16501 gccgcgtccg accccgccat agaaataaag ccctttgggg acttgcgggg aacgcaaact
    16561 cctgcctaaa aaacggtttc ttttgcgttt gaacatcatc aattcggtcc aaagccgatc
    16621 gaggtgttcg atggcggctg cctgcgcgtc gtcgcggatg aagttgggca gttgtgaggc
    16681 agcctgatac caagtcagcg ggctgtgatt ttcaaacggc ggggctttaa aaagttggtc
    16741 tctattactc atatttaacc ttgttatact tcttgcggct aagaaagaaa atgcttaccg
    16801 tcggtattat aaatgatttc cttcaaactt ggcattttcc gtggaaaaga ataatgacgc
    16861 gcacaagggg gtataagcaa aggcggcgtt tcaatatggt gcagatatga gctcaaatgc
    16921 cttttatagt agattaaatt taaaccagta cagcgttgcc tcgccttgcc gtactatctg
    16981 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata atccgtagag
    17041 tcggctcctt ccggctcaga cggcatacgt ttatgccggc acttcttacc gccctcgtgc
    17101 cggctatccc aaaactgctt tgaacactga acgctctttg gtttacttca aaaacgcggc
    17161 gtgataagca atatgctcgc caataaaact ggcgatgaag taatagctgt gatcgtagcc
    17221 tttatggaaa cgcacatcga ccggctggtt tgccgcacgg caggtttcga taaaatcttc
    17281 ggtacgcaat tgtgtcggca aaaactcatc ttccaagcct tgatcgatgc gcataccttg
    17341 cactttatag ccttgttgaa tgagtgagtt agcatcatat tgctgccatt tttcacggtc
    17401 tttccctaaa taagcagtaa aggctttttc tccccacggc acgaggcttg gcgataaaat
    17461 aggcgaaaag gcagaaacac tttgataacg ttcctgattc cgcagcgcca ataccaatgc
    17521 gccgtgtccg cccattgaat gccccataat ggaacgtttg ccgttggtag gaaagtgttt
    17581 ctcaatcaga cggggcagct cgttcaaaat gtaatcatac atttgataat tcgccgccca
    17641 aggctgttcg gtcgcattca aataaaagcc tgcactctgt cctaaatcgt aagcatcatc
    17701 gttcggcact tgctctccgc gagggctggt atcgggggcc accacaatta cttgatgttc
    17761 tgccgcataa cgctgaaagc ctgatttggt aatgaaattt tgttccgtac acgtcaagcc
    17821 ggaaagccaa taaatcacac caagcggtcg attttctgga ttatttggca aatagacggc
    17881 aaatttcatt tcgcattgca gcgtttgggc atgatgcgcc caaacttgtt gcgaaccgcc
    17941 aaaaatttga tgttgttcaa tcagtttcat cgcatacctt agtagtgaat aacggcgcgg
    18001 atcgatttac cttcgtgcat taagtcaaag gctttattga tttgatcgag tgtcattgtg
    18061 tgggttacaa acggttctaa ctcgatatcg cctttcattg aatcttccac cattttcggc
    18121 agttcggagc gacctttcac accaccaaat gcagagcctt tccaaacacg acctgttacc
    18181 aattggaacg gacgcgttga aatttcttgt cccgcacccg ctacgccgat gataatggat
    18241 tgtccccaac cacgatgtgc actttctaat gcctgacgca ttacgtttac gttgccgata
    18301 cattcaaagg tatggtcaat gccccattta ttaatgtcta acaacacatc tttgatcggt
    18361 ttatcgtaat cgttcgggtt caaacaatcc gttgcaccaa actgttttgc caactcaaat
    18421 tttgatggat tggtatcaat ggcgataatg cggccggctt tggcttgacg cgcaccttgc
    18481 accaccgcca aaccaatcgc gcctaagcca aacacggcaa cagagtcgcc ttcttgcact
    18541 tttgccgtat tatgtaccgc accaatacct gtggtaacgc cgcagccgag caaacatact
    18601 tgttcatggt tggcttcagg gttgattttc gccagtgaaa cttcggcaac aacggagtat
    18661 tcactgaaag tcgaacagcc catatagtga taaatcggct gaccttgata agaaaaacgc
    18721 gtcgtgccgt ccggcattaa gcctttacct tgtgtatcac gcactgagac gcacaagttg
    18781 gttttacctg aacaacaaaa ctcacattcg ccacattcgg cggtgtaaag cggaatcacg
    18841 tgatcacccg gttttacgct tgacacacct tcgcccacag caacgaccac acccgcacct
    18901 tcgtgtccaa gcaccacagg gaatacgcct tcaggatcgc ttcctgataa cgtaaacgca
    18961 tcagtatggc acacgccagt gtgggtattg cggattaaca cctcgccttt acgcggcatt
    19021 tctacgtcga tttccacaat ttgtaagggt tggttagggg cgaatgccac cgccgcacga
    19081 gatttgatgg ttgaatcggc ttgtttcatt tccattgtct ttcctcttaa ttcattgaca
    19141 aagttgcctt gcctgagcaa catagcaaca gtttacactt tagagttaac tctaaagcaa
    19201 gtgtttttta ctgtttttgt ttagaaggag ggaaaatgac ttatactact gccaaagccg
    19261 ccgaaaaaat aggcatctcc gcccacaccc tacgttttta cgacaaagaa ggtttgttgc
    19321 ccaatatcgg acgtgatgaa tacggtaacc gctgttttac cgataacgat ttgcaatggt
    19381 tgggcttatt gcaatgcttg aaaaatacgg ggatgagctt aaaagacatc aaacgctttg
    19441 cggaatgtac cgtcattggc gacgatacca ttgaagaacg cctttccttg tttgaaaatc
    19501 aaatagaaaa tgtgaagtgt caaattgccg aattaaaacg ctatttagat ttgcttgaat
    19561 acaaattggc gttttaccaa aaagcgaaag cattaggctc ggtaaaagct gtaaatttgc
    19621 cgcaaattcc tgaaacggct tagttttagt gcaataagca ggatttttgt cctgttcgta
    19681 atagaaatca aaaatttgtt gtttaaaaag cggattgtaa gcaatgatat ag
//
LOCUS       DAWHRA010000022        19705 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWHRA010000022 DAWHRA010000000
VERSION     DAWHRA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.022"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(338..4507)
                     /locus_tag="WIB30_000590"
     CDS             complement(338..4507)
                     /locus_tag="WIB30_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ3796707.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLERFETGKISVGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIHLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTLWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(4562..6409)
                     /locus_tag="WIB30_000591"
     CDS             complement(4562..6409)
                     /locus_tag="WIB30_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ3796708.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHVYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQVGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKGGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRASVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAESRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6819..8048
                     /gene="sstT"
                     /locus_tag="WIB30_000592"
     CDS             6819..8048
                     /gene="sstT"
                     /locus_tag="WIB30_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ3796709.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8160..9443
                     /locus_tag="WIB30_000593"
     CDS             8160..9443
                     /locus_tag="WIB30_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ3796710.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     LQNSAESANQTGNNQPADSSDSAPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLT
                     HCKGDSCNGDNLLDEEAPSKSEFENLNESERIEKYKKDGKSDKFTNLVATAVQANGTN
                     KYVIIYKDKSASSSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGN
                     IFAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGTAVYNGEVLHFHTENGRPY
                     PTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGR
                     FYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9518..9733)
                     /locus_tag="WIB30_000594"
     CDS             complement(9518..9733)
                     /locus_tag="WIB30_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796711.1"
                     /translation="MFKLGVYTCLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9726..9941)
                     /locus_tag="WIB30_000595"
     CDS             complement(9726..9941)
                     /locus_tag="WIB30_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ3796712.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10016..11359)
                     /gene="argG"
                     /locus_tag="WIB30_000596"
     CDS             complement(10016..11359)
                     /gene="argG"
                     /locus_tag="WIB30_000596"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ3796713.1"
                     /translation="MSQNHTILQSLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRVKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            11672..12472
                     /locus_tag="WIB30_000597"
     CDS             11672..12472
                     /locus_tag="WIB30_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ3796714.1"
                     /translation="MNAFNLIIIGDEILHGSSQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHSEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12512..13498
                     /locus_tag="WIB30_000598"
     CDS             12512..13498
                     /locus_tag="WIB30_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ3796715.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWESDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14155..14417
                     /locus_tag="WIB30_000599"
                     /pseudo
     CDS             14155..14417
                     /locus_tag="WIB30_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14455..14829)
                     /locus_tag="WIB30_000600"
                     /pseudo
     CDS             complement(14455..14829)
                     /locus_tag="WIB30_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15002..15358)
                     /locus_tag="WIB30_000601"
     CDS             complement(15002..15358)
                     /locus_tag="WIB30_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ3796716.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(15501..15845)
                     /locus_tag="WIB30_000602"
     CDS             complement(15501..15845)
                     /locus_tag="WIB30_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796717.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(16004..16405)
                     /locus_tag="WIB30_000603"
     CDS             complement(16004..16405)
                     /locus_tag="WIB30_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796718.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(16409..17149)
                     /locus_tag="WIB30_000604"
                     /pseudo
     CDS             complement(16409..17149)
                     /locus_tag="WIB30_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(17219..17536)
                     /locus_tag="WIB30_000605"
     CDS             complement(17219..17536)
                     /locus_tag="WIB30_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796719.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(17533..18135)
                     /locus_tag="WIB30_000606"
                     /pseudo
     CDS             complement(17533..18135)
                     /locus_tag="WIB30_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(18561..18905)
                     /locus_tag="WIB30_000607"
     CDS             complement(18561..18905)
                     /locus_tag="WIB30_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796720.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(18943..19287)
                     /locus_tag="WIB30_000608"
     CDS             complement(18943..19287)
                     /locus_tag="WIB30_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796721.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(19290..19478)
                     /locus_tag="WIB30_000609"
     CDS             complement(19290..19478)
                     /locus_tag="WIB30_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ3796722.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(19548..19705)
                     /locus_tag="WIB30_000610"
                     /pseudo
     CDS             complement(19548..19705)
                     /locus_tag="WIB30_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
BASE COUNT         4621 a         4690 c         5100 g         5294 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacctgtgta
       61 tcgctgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccgg tacggcgttg
      121 cctcgccttg gctcaaagag aacgattctc taaggtgctc aagcaccgag tgaatcggtt
      181 ccgtactatt tgtactgtct gcggcttcgt cgccttatcc tgatttttgt taattcacta
      241 tatcgattct gttcccttgc aggcggacgg cggcggcgga cggtggaaaa gcaggttttc
      301 aaatgtccgg tttggcggtg cgccgtctga aaaccgttta tttccctttg ccgtttccgg
      361 cggagtcttt tttgtccgaa ccgaataggc ggtcgaaacg tatggtgtat gtcagctcgc
      421 cgcccgacga acggctaccg atacgggcaa ccgcctgtat ggcgcgggtc agccggtaaa
      481 tcagtttgac ggactgttcc gcgctggaga tgctgtattc gtagccgatg tagagtttgc
      541 cggtcagttg tttgccgacg gtcagcacct gttcggcggg gttgagttcg ccggtttgcg
      601 cgttgcggct gcgcttgctg gtaaagccca aatcatccac cagcccgatg cggtcgttga
      661 tttgcccggc aagcagcgcg ccggcggctg cggacagggc ggcattgtcg ccgctgctgc
      721 cgctgccggc gcggttgagg atgagccagg agagcttgtc tttttcactc atcggttcgt
      781 ttgccgtcag cgtaatgcgc ggactgttga ggctgcccaa tatttccacg cccgcaccga
      841 cgggggaaag gcggcgttcg gcgcggatgt tgaggttggg gtcgttgagc gggccgacaa
      901 aggagaccgt gcctttggta atgtccaaat cctgcccgta tgccttataa cgccctttga
      961 tgacgcggac cgtgcccacg ccccgcacgc ttccgcccga ttgggcggtc agggtcagtt
     1021 tgccgcctat ggtaacgtcc gcgccgtagc cggcgaagcg gatgccgtca ttgaggtcta
     1081 aagtcaggtt catattgacg gggagcggtg ccgccgcctc ttttttgact tcgcctaata
     1141 cgacgacatc gtcgccgacg gacggcatcg aggatttttg cgaaccgaac agcccctgat
     1201 ccgttttaat catcccggta acggatatgc ctttttgcgg cgaatagcgc aggcgggtgt
     1261 tgccggaaac cgtcaggcgg cggttggggc gggacaggat gcggtatttg tcgaacaccg
     1321 cgccgatatc gacatcgggt ccgctgtttt ccataccgac cgtaccggag agttccgccg
     1381 tcccttcgtg ccggaatttc aggctgtcga ttacccattt cctgcccgcg atatgcgaac
     1441 gcagcgagcc gttgtccaag atgatgcctt gggtttggtt gcggtaatag agtttgtcgc
     1501 cgttgatgct gccgcccaag tgcggatcgg cgatgctgcc gccgagggtt acggcggcat
     1561 tcaggctgcc tttgacggtt tgtccgaccg gtaggaagtt gcggaatact tcggcatcgg
     1621 caacggtcag gttgagcctg ccgcccaaag gcgcggtatc gaaagagcgg ctttgcccga
     1681 cggtgatgtt gccgttgatt ttcccgtagt tgctgctgcc gttgacggcg gcattgacgg
     1741 acggagagcc tacccgtccg ccgatttgcg cggcggcatt caggctgccg gtaatgtttt
     1801 gcgcggcggc gggcagaaag ggcttcaatg cgcccaagtc gggaagggag gcggtaatcc
     1861 tgccgccgag cggtgcattt gccatattgc cgccgaaggc gttggcgatg cccaaatcgg
     1921 cgttaatccg cccgaaacgc gcgccgccgt caagcaggat tccgatacgg tcgttttgaa
     1981 agcgcgtttt cagggaaaat gcgttcaaac ccaaagcctg cccgccgggc aatacggcat
     2041 cgccgctttg ccggctgata ttgaggtagc cgcgcgcgtt gcgcccgtag gcgacatccc
     2101 agtcgccgtt taaaaccaga ttgtgttcga agggcggttt gaagaaattg tgcaactcgg
     2161 cgatatgcag accgtgtgcg ccgccttttg ccgatatgcc ggttttttta tcccaagaaa
     2221 agtgttgcag gttgaggctg ccgcccattg cctgccaatt tgccgcactt gccgccacgc
     2281 gttccgcacc ggcttcgagc gtcatacggt tttgcagctt gaggttgaat gcgccgccga
     2341 tgtcgagaat gccgatgctg cccttccata gggtaagttc cctgttgatg ccgcctgaag
     2401 cgtccaaatc gaatttgaac ggtttgccat ccagcgtcat ggcggcgtgt gtgcggatgc
     2461 ggtgctgcac gcccgtgccg tccagcatca ggtcggcggt atcgacaacc gccgctccgc
     2521 ccgacagcga aaggcggctg cctttgatgt cggcgcgtat cgggcggctt gtgtcgggcg
     2581 aacctttgag cgtgaaatcg agcgaacgga tgtctgccgc cttgccgatg tgcaggttgc
     2641 gcgccgcgcc ggaaaggtcg gtttcaaagg ttcggatgcc gccgtccaaa tcgccggaaa
     2701 ggtgtccgcg tacatttaaa gaccccgcga gtccgaaacc gaaacgggat aaatcgggtg
     2761 cggtgatgtt gaggttgagc cggtcgcctt ttttgccgaa gccgccgtct gttttaataa
     2821 tgttccgccc cagccgcaaa tcgacggcgg cacgcggaag gtggcgggac tcgtaaacaa
     2881 tgtcggcact gccggcaatc ggtacgccgt tgaacgtgcc gggtaaaaac cgcattttgc
     2941 ctgtgaattt ctcttttgcc agttcgccgg caaggtttat tgagccgttg atattgcctg
     3001 ccggaagttg cggattgatg cgcgaagggt cgaatgcgcg ggaacggatg tccagcttga
     3061 gcaggcggtc tttaaacagc tcgagatagc cttgcgcggt caggctgcct tgcccggcgg
     3121 cgatgttgac ggtgtcgagc accagtttcc gctgtccgtt tgcggggtcg cttgcaatgg
     3181 cgaggctgcc gtccgtgcgc gccgtgccga tgccgagttg ccaagagatt ttgggcgagg
     3241 cggtcgtgcc accgatgccg atgctgccgt ccaacctgcc tttgaacgcg gtttgcagta
     3301 cgtcttccgc gccgacggag ttgatgccta tatttaaatc gaggatgtct ttttcggtgt
     3361 cgattttgcc cgacagcctg atgccgcccc gtccgagcag ggcggcggac gtattgccga
     3421 tatgcaccgt gccgtcctgc cggatgacaa agccgcctaa aacctgacgg acggggatgc
     3481 cgttgcggtc ggcaaagccg gctttggtgt tttccaaatc gagcgaacct tccagcgcga
     3541 tgccgtctga aaacgacggg atggcggtca ggtcgaaatt cagtccggca tcgggcaggg
     3601 aaggcacgaa ggcggccgga ttgatgttga accctttgac cagtacttct tccaatgttt
     3661 tatccaatga ttcggcaaac gggtggatga cggattttcc cgagaggtgg atattgccgc
     3721 cgtcgatcgc cagttcggcg cgcacatcct tcaggctgcc gctcagccga gccgtactgt
     3781 gtatggtttt gccttcgagt ccgcctttgg tgtaaatggc ggtatcgagg gcaaacggtt
     3841 ttttcaagcc gaccgaggct gaccccgacg aactgctcca cggcgtgtcg gcggccttca
     3901 ggtcaaggcg gtgtcctttg cggtcgtaac ggtatgccgc gttgaggcgt tcgagataga
     3961 cggtttgttt gtcaaaagtt ttgccgacgc tgattttgcc cgtctcgaag cgttcgagat
     4021 agacggcggc gggcaggtct atgctgtcgg gaaggctgag cggcgggcgt tcttctttag
     4081 gcggagtcgg tttggtaacg atggcgatgt cgccggcgga aatttcggta atgtgcaggc
     4141 tgcggcgcat cagttcggac ggtttccacg cgaagcggaa gcggctgatt ttaaggtctg
     4201 ccccctcggt ttctatcgac cagttgtcgc cgtcgaagcc gtcgagcagc gtgcctttga
     4261 gattttggga ggaaatgttt acaccgaacc aagacgggat ttggtacagc ccgaagcgca
     4321 aacccgcttc cgtaccggcg agccagccga ggaaacatac tgccaaaatc aggacagaca
     4381 gcagtgccgc cgacagcttc agcagcgggc ggcgtttttt tgccggcggg gtggggcggt
     4441 gttcagacgg cattttgcgc gtgccgtttt cggtcggatc ggtatctgtc ggtgcggtat
     4501 cggtcataat gtctgtttca aaatagtttg ctgcaatgcc gtctgaagcg gccgtatcgg
     4561 tttagaagcg tgttcccaag ctgatgtgcc agcggatttt cttatcgctg tgcccgtagg
     4621 cgatgtcgaa ggaaaacggc gcgagcgggc tgaaccagcg cacgcccagt cccgaaccgt
     4681 gtttcagctt catacgtttg aaattggcgg cggcatcgcc catatcgtgg aataccgcgc
     4741 cggaaagggt gcgcgtaaac ggcagttggt attccaggct gcccaccaag agggcgcgtt
     4801 cgggcaggac cgatccgttc gggccggcaa gcccgatgct gtcgagttcg taaccgcgca
     4861 cggaagacgc gccgccgctg cggaacatca gccctgaagg aacgttggca ttgtcgcggg
     4921 caacggtgta acccgcttgt ccgcgtatga tgaacgtgcc gagttttttg ttttcgggcg
     4981 tgaagaaata acctgcgcgg gcagaggtgc ggatcagcgc ggtggaggac aggaatgcgc
     5041 ccaaagtcgt accgattttg ccgtcgaggt aatggccgtt ttcgggatgc agcacgttgt
     5101 tgagcagctg gcgtttccaa gaggcggtca gcatcgtggc gtggctgttg cccaaatcga
     5161 tatccgagcc ggggattttc cggctttctg cgagaaactc cgcccccagc ctggcatcga
     5221 tgcccgcgcg gtcgcgcaca taccagatgc cgccggagaa ggcgcgtttt tcgaggtttt
     5281 gggtggtcga acggttgtag gaaacgttgc ttgtccagta gttgccccga tagttgcgcg
     5341 gctggctgat gccggcggca agcgtggttt cgtatttgtc catatcccaa acgaccgagc
     5401 cgatatagcc tttgttgaag agattgtaat agtcgtaggc gattttgccg cccaaaccgt
     5461 attccgaatc gaggcggatg ccggtttcga gtttgtggcg tttgacctcg gttacgctga
     5521 ctttgacggg gacgcggtcg ccttggaggc ggtcgaagtc ggcttgtacg gacgcgccgg
     5581 aataatgccc gttttgttcg agcgcctgtt ggaagtcgag cagcaggtcg aggtcgtagg
     5641 gcgtgcccgg ttggaagcgc gccaggccgg agacgatttg ttcggggtaa cgctgtgtgc
     5701 cggtgatttc aaagtcgccg aaggcgatgg ggcggccgct gtccacgacg acgttcaaat
     5761 cggcggtggc ggtatcgggg ttgacggacg cccgggtgtt gccgagcttg gcaagcgggt
     5821 agcctttgcg cgttaccgcg ccgaggacgg aagttttgct gttttcccaa ctgtcctgat
     5881 cgaaatcact gcctaccggc tgctgccagt tttccagcgc gttgcggtag tattcggcga
     5941 ggttgccgtc tgaaaggatg tcgccgagga tggcgacacc gacgttggcg attttggtgc
     6001 gcggccccgg tgtgatgtgc accgtataag ccccgccttt ttccgtcagg ctgactttgc
     6061 tgctgaaata gcctttgctg cggagcatgg ttttgacgtt gtccggtgct tcttcggcaa
     6121 ggaagcccac ctgttcctta tccaaaacct cttcctgctg ctgcgtgatg agcggcaggt
     6181 gttcttcgac catatctttg atttcactgt cctgcgtgtc gatgcggacg gggaatttgg
     6241 gttttaattt gactgattcg gtgtcggggc ttttgttttt gaacaatgcg aaacccgccg
     6301 ccttgttttc ggaaaggtcg gcggcaggcg cgtatacgtg cggaaagaaa aataaagccg
     6361 gcaggagcag ggcggtcggt ttgatcatca tggtacgggt gtcgtgcatc ggtgtggcgg
     6421 ctttcgattt taacactgtt cggacggggt ttcgaacggc tgtttttgtg ttgttcggac
     6481 ggggcgggac acttcagacg gcatactgcc gccggtattt cggaggttgc ggcccgccgg
     6541 aaatgtatct gcttgtttta aggtattttg tttccaatcc gcatatggaa aacaggctgc
     6601 gtttcggaat agtgccggaa aataggcagg attaaataaa tttattttcg ttcaataaat
     6661 taaaccgttg cgccgaatga taaagcaaag cctgtccggc actgcatagg gcaaatcaac
     6721 gcaaccttaa aacacagccg aaaaataaaa aaacacagag aatgagtttt cttttaaaat
     6781 acaacatttt ttaacatatc acaacataag gaagaattat ggcgttcggc aaatccttat
     6841 ttcatgcgat cggccgcgtc agcctggtca ggcagattgc cgccggtttg gcgttgggca
     6901 tcgtaatcgg ttcggtttcc ccgcaactgg gcttggcggc aggcttgttc ggcagcctgt
     6961 ttgtcggcgc gctcaaagcg gtcgcgccgg ttttggtatt tattttggtg gcggccacaa
     7021 tcgcgcagca ccaaaaaggc aacaaggcgc atatcaggcc gattatcgtc ctttacctca
     7081 tcggtacgtt ttccgcagcc ctgaccgccg tcatcgccgg tatggttttc ccgacgcaca
     7141 ttgttttggc gggcgcgggc gatgtgtccg ccgcgccgcc ttccggcatt gtggaagtat
     7201 tgaaatcgct gctgatgaac ctggtcgcca acccgattaa cgcgattgcc aatgccaact
     7261 atatcggcat tttggcttgg gctttggttt tgggcgcggc gttgcggaat cacggttcgg
     7321 acgttacgcg gcaggtcgtt gccgatttgg cggaagcggt ttccaccgtc gtgaaatgga
     7381 ttatccgttt tgcccctttg ggcattttcg ggctggtgtc gtccacaatc gcggagacgg
     7441 gtttcggcgc gctggcgggg tatgcgaagc tgcttgcggt gctgttgggc tgtatggcgt
     7501 ttatcgcgct ggcggtcaat cccgccatcg tgtggtggaa aatccgccgc aacccttatc
     7561 cgctggtgtt tacctgcctg cgcgaaagcg gcgtgtatgc cttctttacc cgttcttccg
     7621 ccgccaatat ccccgtgaat atggctttgg cgaaaaaact ggggctgcac gaagacactt
     7681 attcgatttc catcccgttg ggggcgaccg tcaatatggg cggcgcggcg attacgatta
     7741 ccgttttggc tatggcggcg gcgcacaccc aaggcataca ggttgatttt gccaccgcgc
     7801 tgctgttgag cctggtggcg acggtcagcg cgtgcggcgc gtccggcgtg gcgggcggtt
     7861 cgctgctgct gattccgctg gcgtgcagcc tgttcggcat cagcaacgat gtcgccatgc
     7921 aggtggttgc cgtcggcttt atcatcggcg tgattcagga ttcggcggaa acggcgttga
     7981 attcttcaac cgatgttttg tttaccgctg ccgccgattt gggccgacag agaaaccggg
     8041 cggaataaac caagctatgc cgtctgaagc ccgtttggcg ttcagacggc atatttttac
     8101 aattttatgg gcatatggcg gaaacaatac cggctgcctt gaatatgaag gggatgatga
     8161 tgtttgaacg cagtgtgatt gcaatggctt gtatttttgc cctttcagcc tgtgggggcg
     8221 gcggtggcgg atcgcccgat gttaaatcgg cggacacgct gtcaaaaccg gccgctcctg
     8281 ttgttgctga aaaagagaca gaggtaaaag aagatgcgcc acaggcaggt tctcaaggac
     8341 agggtgcgcc atccacacaa ggcagccaag atatggcggc agtttcggca gaaaatacag
     8401 gcaatggcgg tgcggcaaca acggacaaac ccaaaaatga agacgaggga ccgcaaaatg
     8461 atatgctgca aaattccgcc gaatccgcaa atcaaacagg gaacaaccaa cccgccgatt
     8521 cttcagattc cgcccccgcg tcaaaccctg cacctgcgaa tggcggtagc aattttggaa
     8581 gggttgattt ggctaatggc gttttgattg atgggccgtc gcaaaatata acgttgaccc
     8641 actgtaaagg cgattcttgt aatggtgata atttattgga tgaagaagca ccgtcaaaat
     8701 cagaatttga aaatttaaat gagtctgaac gaattgagaa atataagaaa gatgggaaaa
     8761 gcgataaatt tactaatttg gttgcgacag cagttcaagc taatggaact aacaaatatg
     8821 tcatcattta taaagacaag tccgcttcat cttcatttgc gcgattcagg cgttctgcac
     8881 ggtcgaggag gtcgcttcct gccgagatgc cgctaatccc cgtcaatcag gcggatacgc
     8941 tgattgtcga tggggaagcg gtcagcctga cggggcattc cggcaatatc ttcgcgcccg
     9001 aagggaatta ccggtatctg acttacgggg cggaaaaatt gcccggcgga tcgtatgccc
     9061 tccgtgtgca aggcgaaccg gcaaaaggcg aaatgcttgc tggcacggcc gtgtacaacg
     9121 gcgaagtgct gcattttcat acggaaaacg gccgtccgta cccgactaga ggcaggtttg
     9181 ccgcaaaagt cgatttcggc agcaaatctg tggacggcat tatcgacagc ggcgatgatt
     9241 tgcatatggg tacgcaaaaa ttcaaagccg ccatcgatgg aaacggcttt aaggggactt
     9301 ggacggaaaa tggcggcggg gatgtttccg gaaggtttta cggcccggcc ggcgaggaag
     9361 tggcggggaa atacagctat cgcccgacag atgcggaaaa gggcggattc ggcgtgtttg
     9421 ccggcaaaaa agagcaggat tgatgctgat gccgcctgaa gcgggggcgt tcagacggca
     9481 ttgtgatgtt tggggtgggg tccggttcaa atccgcatca gttgataaac ccgtcgtctt
     9541 tcattttctt ttcggaaaaa tactggccga ataccagcag ggcggcgagg atgatgacaa
     9601 acagccccgc catagtcagt gtgattttga agaacaattc cgcttcgaga aaagaaaacc
     9661 agagttgcac gatcagcagc agcacccagc cggcaaacag tccgagacag gtataaacgc
     9721 cgagcttaaa cacgatcccg ctccaaaatg acgacgtagg cctcgcgttt gaaaaacagc
     9781 agcatacggc gcaaatcttt ttccatcgtt acaacccgcc agccttcggg ggtttgtttg
     9841 ttgaggaacg cgctgaaatt gacggggttg acctttgccg cgccgaaaaa caggctggac
     9901 aacaggcttt cctgataaat gacgactttg tattctttca taggattcct tttaacataa
     9961 gggatcatct tttcagacga ccccttaagt tgctaatgat gtaaaacagc aaaccttatt
    10021 gcttattgcc caactgcggc aataccgaac cttcgcccag cgagagcaaa ccgctttgcg
    10081 agtagatacc cagtttgacg cgggtgtcgg tgatgtcgag gttgcgcatc gtgagctgtc
    10141 cgatgcggtc gagcggagtg aacgcagcgt cttcgacttt ttccatactc aggcgttcag
    10201 gttggtaggt caggttgggc gattcggtgt tcagaattga gtagtcgttg ccgcgccgca
    10261 gttcgagggt aacttcgccg gtaacggctt tggcaaccca gcgttgtgcg gtttcgcgca
    10321 acatcagggc ttggctgtcg aaccagcggc cttggtagag caggcggccg aggcgcaggc
    10381 cgttgatgcg gtattgttcg atggtgtctt cgttgtggat gccggtgacc aaacgctcgt
    10441 aggcgatgtg gaacaacgcc atacccgggg cttcgtagat gccgcgcgat ttggcttcga
    10501 tgatgcggtt ttcgatttgg tcgctcatac ccaagccgtg gcggccgccg atgcggttgg
    10561 cttcgaggaa gagttcgacg ggatcggcgt attctttgcc gtttagtgca accggcacgc
    10621 cttcttcaaa gcgtacgctg acttcttccg ggctgacttc gacgttttcg tcccaaaagg
    10681 caacgcccat aatgggtttg acgattttga tgcccgagtt caaaaattcc aagtctttgg
    10741 cttcgtgggt ggcacccaac atattggaat cggtggagta ggctttttcc accgacattt
    10801 tgtagttgaa gccgttggca atcagaaatt cgctcatttc gtgacggccg ccgagttcgt
    10861 cgataaattg ctgatcgagc cagggtttgt agattttcag cgcgggattg gtgagcaaac
    10921 cgtagcggta gaaacgctcg atgtcgttgc ctttgtaggt gctgccgtcg ccccaaatat
    10981 tcacatcgtc ttctttcatt gcggaaacaa gcatagtgcc ggttacggcg cggcccagag
    11041 gcgtggtgtt gaaataggcg atgccgccgg tggaaacgtg aaacgcgccg cattggatgg
    11101 cggcgatgcc ttcgtgtgcc aactgcgcgc ggcagtcgat taagcgggcg ttttccgcac
    11161 cgtattccat cgcttttttg ggaatggcgt tgtagtcgtc ttcgtcgggc tggccgaggt
    11221 tggcagtgta ggcataaggc agcgcgcctt tgagtttcat ccacaacagc gcggcagagg
    11281 tatcaagacc gccggagaag gcgatgccga ctttctgacc gacggggagg gattgcagaa
    11341 tggtatggtt ttggctcata agggtatcct tcggacttga aaaaaggagt tcattatctt
    11401 ataaatataa cccattgtca gccgttaagg ctgtctgaaa agaagctttg cccgtttcgc
    11461 tgaaaatctt ttcagacggt ctttacgcca tcctcaacct gactccctcc cctgcggggg
    11521 agggttgggg agagggtaca gacagcaggc cttaagcaaa cgctgcctga aaaacgcaag
    11581 ctcgaccctc tccctaaccc tctcccacgg gagagggaac aaatcacagc accatcgaca
    11641 ttttcagacg gcatcggtaa agaaaccatc tatgaacgcg ttcaacctca tcatcatcgg
    11701 cgacgaaatc ttacacggca gcagccaaga caagcatttc gcctttttca aatccctgct
    11761 cgaatccaaa gggctgaagc tcaatcaggt gcaatacctg cccgacgaac ccgatttgct
    11821 ggtcaggcaa ctgcgccgca gcttttcaga cggcatgcca actttcgtta ccggcggtat
    11881 cggcgccacg cccgacgacc acacccgcca agccgcagcc gctgctttgg atttgcccgt
    11941 cgtccgccat tctgaagccg ccaagtttat cgaaggcata acccagaaac gcggcgagcc
    12001 gcttgattcg ccggaacacg cccaacgcct gaaaatggca gattttcccg aaggcgcgga
    12061 attggtgccc aatccgttta acaacatcgc cggattttct atccgcgagc attatttctt
    12121 ccccggcttt cctgtgatgg cgcacccgat ggcggaatgg gtattggaaa cttattacgc
    12181 cgaccgcttc aaccaaaccg aacgcggcag ccgcagcgtg tatgtgtttg agcagcccga
    12241 atcgcgcatt acgccgctta tggagcatat cgagcaaact tatcccggcg tgcgttcgta
    12301 cagcctgccc agcgtcggtt ggacgcattc agacggcacg caggtcaaac cgcacattga
    12361 gttcggcatc aaagccgaag gagaagccgt caaccttttg gacgcggcgt gggcggaagt
    12421 gttgcacagc ttggacggat tgggagcaga gctgaaaaat cgggtaaact gaaaaccgtt
    12481 tgcccaaatc ggacaatatt taaggaaaac catgcaatac cgaatcagaa gagaaaacga
    12541 agcacccgaa gcaaaaaatg cgggggaaac cttatgggag agcgacatca tgcgcgaagt
    12601 gctgttgtcc gcctatcagg acaggcgcag ggagcggatg tggaaaaaca tctggcgcgc
    12661 cgtcagcacc ctgattctgg ttgccctgat tgcaggcatt ttccgaaaag acgaagccgc
    12721 attgcagttg gcgggcaaca cgccgcatac cgcagtcgtc aatctgtacg gcgaaatcgg
    12781 caacggtgta gaagaccagg tcaaaaaact caaagacggt atggaggccg cctacaaaaa
    12841 cccgcaggca aaagccatcg tcatccgcgc caacagcccc ggcggttcgc ccgtcgtgtc
    12901 caacaccgct tttgaagaaa tacgccgtct gaaggcgcag caccccggca ttcccgttta
    12961 tctcgtggcg gaagatatgt gcgcgtccgg ctgctactac atcgcggcgg cggcggacaa
    13021 aatctatgcc gacccgtcca gcatcgtcgg cagcatcggc gttatcggca gcagtttcga
    13081 cgcgaccggc ctgatggaaa aaatcggcgt gaaacgcagg gttaaaattg caggcagcaa
    13141 caaaggcatg ggcgatccgt tttcgcccga aacgcccgaa cagtcaaaaa tctgggaaga
    13201 aatgctgacc ggcatacacg gcgagttcat caaagccgtc aaaaccggac gcggcggccg
    13261 cctcaaattc cgacaatatc ccgacgtgtt cagcggtcgc gtgtacacag gcgcggatgc
    13321 gctgaaagtc gggctggtgg acggactcgg caatatttac agcgtggcgc gagacgtggt
    13381 caaagccccc gatgtggtgg attacactcc gaaggacgat ttcggcagaa tcttgggcag
    13441 acgcttcggc gcagaactca aagcaagcgt gcgcgaagcc ctgcaggccg tccggtagcg
    13501 ttgtttacag tatttctaag ggaataccaa aataaaaata tacacaaaaa cactcacctc
    13561 cccagtatgc tgccactaag tcagccgcgt cactatttac cgtgcactta aagccgtaaa
    13621 aggcaggttg tccccgggag gttttgtgca tcctgatacc gatcggttgt tgttgctcaa
    13681 aggacagaag gccgctgata aacgagatta cctgtttgtc gctattgacg atttttatac
    13741 tctgccattt tgccggacaa aaccgcagac agtgctgcca agtttctgac cgaacatctg
    13801 tccgacccct gcttgtacct gattgagtac gtttactctg acaatgatag gtaatataaa
    13861 gagccgtcca acatgctttc ggtgcagctt gttatgataa tgggattggt tggaggcttg
    13921 cccgatttgc ttgtccgcag accaacggta aggcggagcg ggttatccgt accttgatgg
    13981 agatgtggca tgaggaacag tcgtttgaca gtccggaaca tcggcaaaag gagttgtgct
    14041 gctttattaa tttaggggct gccctagatc actagggaaa ttcaaattaa gttagaatta
    14101 tccctatgag agaaagtcgt ctaagacggt ataaacaaaa taaactcatt ttgttatcgt
    14161 ggctatgatg tattggatgc gggcgaattt agccatttcc gtatcaatca cagcacacat
    14221 tttgccgaac gacaaaacca tattaatgga actgagaact tttggaatca agcaaaacgt
    14281 catttacgca agtttaacgg cattcccaaa gcgcattttg agctgtattt aaaggagtgc
    14341 gaatggcgtt ttaacaacag tgaaataaaa gttcttgttc cattttaaaa caattagtaa
    14401 aatcgagttt atcctagttg tccagggcag cctcatctct ttatagatag ctgcttacca
    14461 agtatcctta attgctaatt caagccaatc ttcaaaacta ttaaaaaatt catggttttc
    14521 tatattatct aaattaatga caataaccct agaatttcct gttacatcat taccatcaaa
    14581 ataagcttcc cattctccat tactagcgaa tgggataaga tttaaatcag gcaggataga
    14641 atttctaaat atataagcta cttctgcccc ttctgttcca taattctcaa accactagtg
    14701 aaaattatgt ggctgaatat aaggtattaa agatgtatcg ttagagatac gaacatactt
    14761 ttcaggataa caaaaccttt caggaagcat attactagga tataaattaa attcattttg
    14821 aataatcatt tcgttacctt aggatcataa atactaccat taggaaactg ggtagaccaa
    14881 tttggtggtg ctagttcatt ttcaatttgt tttttaattc taaattgaat agcttctccc
    14941 catgtttgcg gcatctaatg ctgcttgctc tgcatcaata ttactaaaaa ttggattagt
    15001 ctcaaaaatg aaaaattact gttttttcat tttttatagc atattcaagt acatgaataa
    15061 tatttttcac taagggctct gtagaaaaat ctgaattttg taatttttct tttacagcca
    15121 aaatactttc cttatctatt agccaatccc cctcacttgg tccaaataac caattatcgg
    15181 taatttcaga aacaagtctc cataatccac gattttgata ttcaataatt tgattttccg
    15241 taatttctac aactgtttca gattgaatgt ttttataagc ccaatcaatc atttcagtat
    15301 attcactaat tgaaacattg tcttttaaac agaaaattaa caggttgtgt tcattcattt
    15361 gtctaagttc ctgatatgag tttgttgttg taaataatct tttgcttgct taccttgtaa
    15421 attaaaggat taaccgcacc ttggatgatg ccgcgaatat catccatacg gttaccccac
    15481 caatttttat tggtgtatcg ttaatcattt atttcaaaaa agttatttat tatcatatta
    15541 tcagggagca ggtcgttaaa aaaaaccccc tcaaattcca ttaaattagt atttatccag
    15601 cacataaacg aaccaaagcg ttctttccct gagtgtaaat atttaaaatt aaactcaaaa
    15661 taagtttttt tgcttagaaa atttaatggt ttttctatag tgaaaaaaaa gcccttactc
    15721 gttttcgttc gagatactat tttaaataaa tttttatttt caataaaata agaatattca
    15781 tttactaagt tgttatgata aaaaagtaat tttattaaaa tgttttcata attttctagg
    15841 ttcatttcta gctcaattta aaatgatttc attgttgccg taccaatgcg aaatttacca
    15901 tcaacaaaat ttcccttcac tgatacaggt acaccatcaa cttgggtagt tttattttca
    15961 ggctgcctga aagaaagata ggcaacttat tactcatctt acgctagccc tcaataattg
    16021 aatacgttgc actagaacca attatttcta tgaatgtttg ctcaaacata aaaatatagt
    16081 atttaaagaa ttctctattc aaaggaaaat aactttcttt caatatccat tccgcataac
    16141 actcatcaag aagttcaaaa aaagattgac caccattttc atgaagcaaa gctctatctg
    16201 catcttccga aattaaatcc ataaaagtat ctataaaata cgattcatca acaaccctaa
    16261 aatgaataac atcttcaata aaaattttta tggttttatc cgtatttcta taaccaactg
    16321 ctataacttc tactttatca gagtttattg ctgcattatc aaaatagaaa tagccagttg
    16381 ggaaatctaa gctttttaat ttcataattt atcgtgtatt tccatatctg atttttattt
    16441 ttcggttatt actaattgtt atttcaagtg ttggttctcc accactacta tcattacgag
    16501 cattaaccgc acgcccatca ggtaaattgc ccgttatcgt tccattagga cgtgtttgta
    16561 catttcgaac attcagacta ttaaaatcat ttagtgcgtc attgtaatcc cccactttat
    16621 catactgaac agcgggacct tttgttttac tcctttgagt agtattgctc aaaatgtctt
    16681 tgatttgtaa atcactgcgc gccttaccgg cttctatgcg agctgccaca ctactcgggc
    16741 tataggggtc gctatcaatc gcaggattac caccgctggt tttttgtccg ctagcctgtt
    16801 tcccaacctg ctgtactgta tcgccaattt catctaccgt acctaacgcc ttagtctgca
    16861 tatggcgaac agcaggtttt ttatagccgg tatttttaac ccaatcccat ttggtcgggt
    16921 taagttctac ttttttacct ctccaaaccg tacctgcggc ctctgctacg gcaagggcag
    16981 tttgggctgt tgctgttata ttcggatggg catcagccca ttgtctggcg gaattgatgc
    17041 cgtcttttac cgcaaaagca ctgtcctgta atatgctcgc ggcagcaagt tgtgcttccg
    17101 ggcttaattt tcctgaatta ttaatacctt gtagagtctg ctgcgcagag atgtagcaac
    17161 tgtattttta ccccgtcggg taaaaataca gttgctacgg ctgctgggaa agcagttact
    17221 attttcccag tggctcaaaa ttaattgttt cagcagatat ttttataata atccctacat
    17281 caataataat ttcactataa aattggtatt tatatagctc tatttcgggt ataaacaagg
    17341 gaaacaagct atcttcctta tcctcataat ctacgaaatt atttgtatct aatttaaaat
    17401 tcaaaatatt actgcatttg atatatccga aataattacc ttcaattgta tctgtaaaag
    17461 ataatgtaag ggtatttcca ttatcggaaa agattatttc ataaaggctt acattgtgaa
    17521 aaatattttt tttcatttga caaaatatcc agaatgatga tgattatttg gcaatggatt
    17581 gccttcataa tcttgtcgtt gcggatattt tccctgtaca ccatcggttc gcggcactct
    17641 ttgggttctt ataacctctc catttggacg aatatctatt ttcacgccat caggaccttt
    17701 ccatgtttca tattgagccg catctgaacc agtacgtcta aaaccttgtt cttgcaatgc
    17761 ttgacgtaat tcatctcgcg tagttggtct gatagattta acaactggct tgtttcctcc
    17821 tgccatctca ccgtccaacg tctgcatagg gcgggcagca ggtgttttat agccgttatt
    17881 tttaacccaa tcctatttgg tcgggttaag ttctactttt ttacctctcc aaaccgtacc
    17941 tgcggcctct gctacggcaa gggcagtttg ggctgttgca gttatattcg gatgggcatc
    18001 agcccattgt ctggcggaat tgataccgtc ttttaccgca aaagtactgt cctataatgc
    18061 gcttgcggca gcaagttgtg ctttcggact taaatttcct aaatcattaa taccttgtaa
    18121 agtttgctgc gcagagatgt aggtcggata tttgtatccg acaaaaatat ttgacgcatc
    18181 tatcgtttcc gaaatgccgc tgttggaaaa tgtcggattc gagaatccga cctacggcta
    18241 tttcgtttaa gtcataaata gccattcatg ttccttctga atttgctttt gctatcctta
    18301 gaaccaatca tataaacatt atcatcatta attaatgttc taagtttatg aagcggtcaa
    18361 atctgtcaaa ctaccagaga tgaagcatat cctcctggaa gtaattctgt tcgtgctgtt
    18421 tccatatctt ctttgccatc atcagttact tctctgtcta aataaaatct tacgataagt
    18481 ttttgtctaa tttcatcaaa ttctgccgta attaatctta tattgatatg aatacacggt
    18541 tcatacctgc tattagccag tcaggtgtag ttggatgggg attcattttt aataatatct
    18601 tcaagcaagt gaaatgtaag aaaagcgtag atacttgaag gccggctatc tgattggcaa
    18661 aaagaaattt cggagataaa gaatttccgt ttatattttt cataaattaa atcaacacct
    18721 tgttttacat aaaaactaac ttcctcagga aggatattta taacattctc tccttttact
    18781 tttatagtta atggtttgat ttttatgttt tcatgaatat ctgataggat tatttctaaa
    18841 aaattatttt tatttccagt cattctggct attttatgta tatttccatt gtgttcgatt
    18901 atcattttat tttttccaag gatttccttc tatattgata gtttatctaa agatttcatt
    18961 taaaatatcc tgactaattt cttgtttata taattctaca attttctgtt tctgtgtatt
    19021 tgtataaaat ttttcgttgg tatctgcttg atattttaat tgtctcaata tttccatgat
    19081 ttcatattct gatttttcat catattcttc aatcatgtaa tcaacaaatt gttttagaat
    19141 atcaaaggct tcgtaacttt ccaaaatttt aatatgttta tcggcaaaat tggacatata
    19201 ccattcgtca gtgattggat tagtatttaa atcatctaaa aaaacattta aatcatctaa
    19261 aaaaacattt acatcatcta aaaacatgac taatccttaa acttaaaatc tgttttctca
    19321 ccggttcttg agttttcaat gtaatgtatt tcaattttgt ttacgttttg aacacgctta
    19381 acccaaccgt cttttgctaa ccaaccattt ttagtatcgg acataggagg tattctcgca
    19441 ggagttttgc cctgtgggtt tgccttaact tcttccattg ctaattgttc ctttaaatta
    19501 ttaggtattg tacgaccggt tgatttttca acagattgtg tccctatttt taatgatttg
    19561 tttcccccag ccatttcccc gtctacagtc tgcatagggc gggcagcagg ttttttatag
    19621 ccggtatttt taacccaatc ccatttagtc gggttaagtt ctactttttt accgcgccaa
    19681 accgtacctg cggcctctgc tacgg
//
LOCUS       DAWHRA010000023        19576 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWHRA010000023 DAWHRA010000000
VERSION     DAWHRA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.023"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..291)
                     /locus_tag="WIB30_000611"
     CDS             complement(<1..291)
                     /locus_tag="WIB30_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3796723.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLIIRIDFNLFCRFDELS"
     gene            complement(463..1632)
                     /locus_tag="WIB30_000612"
     CDS             complement(463..1632)
                     /locus_tag="WIB30_000612"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ3796724.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1815..1925)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1978..2874
                     /locus_tag="WIB30_000613"
     CDS             1978..2874
                     /locus_tag="WIB30_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ3796725.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKVVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     TEYWIRRFPTQYLFMYNRYKAP"
     gene            complement(2918..3982)
                     /gene="tsaD"
                     /locus_tag="WIB30_000614"
     CDS             complement(2918..3982)
                     /gene="tsaD"
                     /locus_tag="WIB30_000614"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ3796726.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRFEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(4099..5286)
                     /gene="ccsB"
                     /locus_tag="WIB30_000615"
     CDS             complement(4099..5286)
                     /gene="ccsB"
                     /locus_tag="WIB30_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ3796727.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(5279..7231)
                     /locus_tag="WIB30_000616"
     CDS             complement(5279..7231)
                     /locus_tag="WIB30_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ3796728.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQESKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7508..8131)
                     /locus_tag="WIB30_000617"
     CDS             complement(7508..8131)
                     /locus_tag="WIB30_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ3796729.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            8337..8966
                     /locus_tag="WIB30_000618"
     CDS             8337..8966
                     /locus_tag="WIB30_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ3796730.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(9087..11483)
                     /locus_tag="WIB30_000619"
     CDS             complement(9087..11483)
                     /locus_tag="WIB30_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ3796731.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11635..12750
                     /locus_tag="WIB30_000620"
     CDS             11635..12750
                     /locus_tag="WIB30_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ3796732.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12753..13352
                     /locus_tag="WIB30_000621"
     CDS             12753..13352
                     /locus_tag="WIB30_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ3796733.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13353..14000
                     /locus_tag="WIB30_000622"
     CDS             13353..14000
                     /locus_tag="WIB30_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ3796734.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            14018..14563
                     /locus_tag="WIB30_000623"
     CDS             14018..14563
                     /locus_tag="WIB30_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ3796735.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14582..16867
                     /locus_tag="WIB30_000624"
     CDS             14582..16867
                     /locus_tag="WIB30_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ3796736.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
     gene            17769..18281
                     /locus_tag="WIB30_000625"
     CDS             17769..18281
                     /locus_tag="WIB30_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ3796737.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            18361..19440
                     /gene="aroB"
                     /locus_tag="WIB30_000626"
     CDS             18361..19440
                     /gene="aroB"
                     /locus_tag="WIB30_000626"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ3796738.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT         5214 a         5584 c         4528 g         4250 t
ORIGIN      
        1 gctcagttcg tcgaaacggc aaaacaggtt gaaatcgatg cggataatga ggctgtgttc
       61 gagttcggga tcggagaggc tgtgccattg tccgagcagg acggctttga acatggacaa
      121 cagtggatag gcaggacggc cgcggtggtc tcggaggtaa cgggttcttt gacgattcag
      181 gtactgttcg atcggctgcc aatcaatcac ctggtccaac ttcaatagcg ggaagcggtt
      241 gatgtgtttg gcaatcatgg cttgggcggt ttgccggaag aaggtactca tggaaaatcc
      301 cctaaatgtc ttggtgggaa tttaggggat tttggggaat tttgcaaagg tctctatctg
      361 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata taaaaatgcc
      421 gtctgaactg ttcagacggc atttttgatt ttcaaaccgg aattacagcc ccgctgccgc
      481 cctcaatgca gcagctttgt cggtgcgctc ccaagtgaac tcaggttctt cgcggccgaa
      541 atgtccgtaa gcggcggatt tactgtaaat cgggcgcaag agatcgagca tttggacgat
      601 gcctttgggg cgcaggtcga aatgttcgcg aactaaggta atcagttttt cctcgctgat
      661 tttgcctgtg ccgaaagtgt cgatggaaat cgaagtcggt tcggcaacgc cgatggcgta
      721 ggaaacttgg atttggcatt gggttgccaa accggcggcg acgatgtttt tcgcaacgta
      781 gcggcaggcg taagcggcgg aacgatccac tttggacggg tctttgccgg agaacgcgcc
      841 gccgccgtgc ggagccgcgc cgccgtaggt atcaacgatg attttacggc cggtcaaacc
      901 gcagtcgcct tgcgggccgc cgatgacgaa gcggccggtc gggttgatca ggtatttggt
      961 ttcgtcggtc agcagttcag acggcaggac gggcttgata atctgctcaa tgacggcgtt
     1021 tttcaactct tcataaccga tttccgggtc gtgttgcgta gagaggacga cggtatcaat
     1081 gcgttttact ttgccggatt cgctatcgta aaccacggtc agttgggctt ttgcgtcggg
     1141 acgcagccaa ggcaggaggc cgtctttgcg taattcgctt tgacgctgca tcaggcggtg
     1201 gctgtaatag atggcaaacg gcatcagggt cggggtttcg tcgcaggcat agccgaacat
     1261 caaaccttgg tcgccagcgc cttggttcaa gtcaatgcct tcgccttcgt tcacgccttg
     1321 ggcgatgtcc ggagattgct ggtcgtagta cacgccgacc gcgcagccgt tggcatcaaa
     1381 gcccagctcg gaggagttgt agccgatgcg tttgatggtt tcgcgtgcga ctttgatgta
     1441 gtccacttgg gcggtggtgg tgatttcgcc tgccaatacg cacaagcctg tgttgaccaa
     1501 agtttctgcg gcgacacgcg ctttggggtc ttgcgccaag atggcatcca aaatcgcgtc
     1561 ggatacttgg tcggcaactt tgtccggatg gccttcggat accgattcgg aagtaaacag
     1621 atattcgctc attgagattt tccttgaaaa acaaaccatc ttcttcagac ggcatgttgt
     1681 atgaacataa tgtcgacagc gggaaatata gcaaaatttc cctattcata ccattcagtt
     1741 gagaaatatt cccatttgaa tggcactttg gaatctctgc ccgtacgttt cttacaggca
     1801 aaaaaattcc cgcatcaagc gggtttggat tgctctggtg agccacatcg gcttttcaac
     1861 cgtccacctt acttttcctt ttgaaaagca ggttggcatg gaattcccaa ctcttaatgc
     1921 agcgcggatg gtagcagaaa agcctttccg ccgcaatact tccctttttc agacggcatg
     1981 tttcgtttac aattcgggct gtttccccct ttgcgaaccg ccatgcacat cctgttgacc
     2041 gccctgctca aatgcctctc cctgctgcca ctttcctgtc tgcacacgct gggaaaccgg
     2101 ctcggacatc tggcgtttta ccttttaaag gaagaccgcg cgcgcatcgt cgccaatatg
     2161 cgtcaggcag gcatgaatcc cgaccccaaa acagtcaaag tcgtttttgc ggaaacggca
     2221 aaaggcggtt tggaacttgc ccccgcgttt ttcagaaaac cggaagacat agaaacaatg
     2281 ttcaaagcgg tacacggctg ggaacatgtg cagcaggctt tggacaaaca cgaagggctg
     2341 ctattcatca cgccgcacat cggcagctac gatttgggcg gacgctacat cagccagcag
     2401 cttccgttcc cgctgaccgc catgtacaaa ccgccgaaaa tcaaagcgat agacaaaatc
     2461 atgcaggcgg gcagggttcg cggcaaagga aaaaccgcgc ctaccagcat acaaggggtc
     2521 aaacaaatca tcaaagccct gcgttcgggc gaagcaacca tcgtcctgcc cgaccacgtc
     2581 ccctcccctc aagaaggcgg ggaaggcgta tgggtggatt tcttcggcaa acctgcctat
     2641 accatgacgc tggcggcaaa attggcacac gtcaaaggcg tgaaaaccct gtttttctgc
     2701 tgcgaacgcc tgcctggcgg acaaggtttc gatttgcaca tccgccccgt ccaaggggaa
     2761 ttgaacggcg acaaagccca tgatgccgcc gtattcaacc gcaataccga atattggata
     2821 cgccgctttc ctacccagta tctgtttatg tacaaccgct ataaagcacc gtaacgaaaa
     2881 taaaaatgcc gtccgaacaa cttcagacgg cattttgtca tctgacgatt tccgacagcg
     2941 gccagcgcgg acggacattg aacgcaccga cctccctgcc cttgcccagg tgcatcgcac
     3001 cggcaaaggc aatcatggca ccgttgtccg tgcagtatgc cgtcggcggg aaaaacacgc
     3061 tgactttttc ggacggatgt ttcggcttgc ctttgggggt cgggatttgc accgtcatgt
     3121 tgccgaaagt ttcacggagc ttgcggtttg caccgacccc gccggcgacc actacggttc
     3181 tgaaccctgt ctgcaacagg gcttttttca ctttcgccgc caacacatcg actaccgcat
     3241 cttgaaacgc acggcagatg tcgttgcgtg tctgctcagg aatgtcatcc gccccgtttt
     3301 ccgcgcgcac tttctcgacg gcggtcaata cggcggtttt caaacctgaa aaactcatct
     3361 gcaaatcgtc ggaatgaatc atcgggcgcg gaaaaacaaa cgcttcgaac ctgcccgatt
     3421 ccgcaagttc cgacagtttc gcaccgcccg gatacggcaa gcccagcagt ttcgccgttt
     3481 tgtcgaatgc ctcgcccgcc gcatcatcga cgctctcgcc caaaagctcg tagtcgccta
     3541 tgcccctgac cgccataatc tgcgtatgcc cgcccgaaac caacagcgcg acaaaaggaa
     3601 agtcgggttt ttcctccgcc aacagcggcg acagcagatg tccttccaaa tgatggacgg
     3661 ggatgacggg cttgcccaat gccaaagcca gtgcattggc gtagctcgaa cccgccagca
     3721 gcgcaccgcc caaaccaggc ccctgcgtaa aggcaaccgc gtcaatgtcg ccatacgatg
     3781 cgcctgcctg cgccaaacag ccttcagtca acggaacaag gcggcggata tggtcgcggc
     3841 ttgccaattc cggcacaacc ccgccgtatt cggcgtgcat tgccatttga gtgtgcaggc
     3901 agtgcgcccg caatccacgt tccgtatcgt aaagcgcaac acctgtttcg tcgcaagaag
     3961 actcgattcc taataccaac atggtctgat gccgttaaaa actgaaaaac gtattttagc
     4021 ggatttcggc acgactgccg tatcccaaaa acggaacatg ccgtctgaag accgttcaga
     4081 cggcatcgtc gcaccgtatc aaagcgttcc gtaagaatgc agcccgctca aaaacatatt
     4141 cacgccgata aaggcgaatg cggttacgaa caaaccgata atcgcccacc acgccagcac
     4201 tttgccgcgc caaccggcaa ccagccgcaa atgcagccac acggcataat tcaaccaaac
     4261 gataaacgcc cacgtctctt tcgggtccca actccaatag cgtccccaag catccgccgc
     4321 ccacagcgca cccaaaatgg tggcaatggt aaagaacaga aagccgacgg caatcgcctt
     4381 atacataact tcctcaatca acacggattg cggcagccac gatttcccgc cttttccttc
     4441 cgcacgcagg gaaaccagtt cggcaatacc gagcatcgcg gaaatgcaaa acgcgccgta
     4501 accgataaag tttgccggaa cgtggatttt catccaccaa gattgcaatg ccggaatcag
     4561 cggctggatg gtatgcgcct cgcgggacac gctgtaccac aagacaaatc caaccacgac
     4621 cgccataaag ccgaacacga agccgcccaa tttctgtatg gcgaacttac cttcataata
     4681 aagatacatc agcgcggtaa tcaccaaaaa caggatgaac acttcataca ggttggaaac
     4741 cggaatatgc cccgcatcgg gacggagcag atagctttcg tgccaacgta ccagcagacc
     4801 ggtaaagcct gctacggcag acacccatgc aaacacggtt cccataccca acagcgtgtt
     4861 ggtcggcaca ttttttacgc ttgccaaaac cgcgcccgaa atataggcga acagggcgaa
     4921 gaagacaaag gcgcactgcc acataatcgc cgactggctg ctgaggaaat accgcaacag
     4981 gaaaatctct gccgatttga tgttgccgcc atacagaccg acggcggcat aggcaagcaa
     5041 tacgcttaaa ggaacaaacc agcgcatcgg tttgaaaaac caacccaaaa acacggcaat
     5101 accggcactc gcccacaaca tgaccgtttc gtaaatgtcc atatgcatac cggaacgggt
     5161 ctgtacgaaa accgtagccg caaaaaccag cacggcaaat acccaatccc aaagattcag
     5221 attgctgatc aaagacttct gaatcagcag ctcgtgttcc ggaagggttt tatagtgttc
     5281 agtcatgatt caagtccttg ccgagccgtt gcagactctc gacgtgtttt ggaaattcct
     5341 tctgcaaatc ccgttcactg cgggccgaag acatggcaaa acggattttg ccgtctgaaa
     5401 acaataccca cgcccgtttt tcgcgcacat aaaacatcaa taccgtaccc aataccaaca
     5461 gcaccgagcc gagatagacc aaaagcgcac ccggggaacg ggtcatctgc aaacccgacg
     5521 aacgcacctc ggaaaacccg tcaagttgca gcagcatagg cgcgggatat tcggtcaaac
     5581 ccgtgtacgc atccatactg tgcagcagga aacgattccg cgcttcatcc tgctgccatt
     5641 cgggcaagcc gtaccggcgt atggtttcat ccaaagcagc gttcatcacg ccgtaaagca
     5701 tttcgtagaa atagccctgc atcttatcct gctgctcttt cgggatattg gacgtaataa
     5761 attcgtccaa tcccaaatag cctttttgtg caaagatgtt cagcgtgttt tccgcagcca
     5821 gcatgaattg ttcgcggatt tcggcaggtg cgtctttggt tgcgtcggca accagacgtt
     5881 tgcgcccttc cccatctttc aaaaactcac gcaatgccat aaaggtgtcc gctttcaact
     5941 gcttgtccaa ggggatacgc agccagcggt attgctgctg caagccgctg cgcgtgccgg
     6001 taatccaaaa ataatcctgt tcctgcaaaa ccggcagcat atagttttta tattcgaccg
     6061 cctgccctgc cgcatcacgg atacggtaaa caatggaagg gccgatattg gtgtattttt
     6121 tactttcctg agtaacggcg cggacatcgt tcagcgtgga tttcaggctt ttttcccgtt
     6181 ccgcgccctc gctcatgtcc tccacattca tagaagtgaa ctgatcgaac tcaagacgat
     6241 atttgtgttt gccaatttcc aacggaaact ggtgtatgga tgttgccttc aacacgacag
     6301 gctcgcgcga agcatcaccc aaattccacg ccttgaatgt caaatccgaa ccgccgtcgg
     6361 caaaactcgc ctgataaatc gtgatgccgt gcaaggtcaa aggatggttc acgcggatgg
     6421 tgcgctcgag tttctcaccg gttgccttgt ccgtcacttc aatatcgctg gcgaaatcac
     6481 gcggcatacc cgtattgtaa aaatcgatat ggaatttttt cagtttgact tcaaaaggca
     6541 agtcctgaac caatatcccg ttgtcggcat tcaggaaaac cacatccgca ctctgcccct
     6601 cggaaatatt gacgttgccc ctaaatgaga gattggacgc actcaaaata ctttcgggct
     6661 tgaaatcctt ggcataaacc gcctgattgt ccggaacaat ccgaccggtc agcataccca
     6721 gtttcaacag caggttactg tctatcaacc cgcccaggca aatgacaatc aaagcaacat
     6781 gggcaaagat atagccccat ttgttcattg tgcctttttt ggcggcaatc agaaccgacc
     6841 cgtcttcacg gttaatggtt tttccctgaa aaccttgtac ttccagataa cgtttggcaa
     6901 cctcgggcgc aatttttaca tccaacagcg aagaatggcg catcgccgcc agagattttt
     6961 ctttaacctt ttcccgaaaa gacttcattt cgcgccagaa cggcggcaca ttgcgaatca
     7021 ggcacaaact ggtagaaacc accaaaaaca tcatgataac gacaaaccat gccgaagcat
     7081 agacgtcata cagtcccaga aaaccaaaaa tctgcgccca aaaaggtccg aatttgacca
     7141 aataatccgt ctgcggctgg ttttgctgca acaccgtacc gataaccgat gcaataccca
     7201 gcagactgag caaagcgact gcaaagcgca tggagctgaa aaaagcgaac cacggacggg
     7261 aaagaagtgg gggagatcta cgggatttac tcattgtgtg tttattccgc catcaggaat
     7321 atgggaaagc agaattgggc aaacagaaaa caacgtcccg attctactgt cttgatgctt
     7381 tttatttcaa gacaatgaag acagcctgca tcgattccaa cggttgcgat tgaaaaaact
     7441 tatcgcagaa ttgcctgaag ccgtctgaaa acttttcaga cggcctctaa aacagactat
     7501 tgcggaatta acgcaaacct tggataaagt tggcgaccgc tttcaaatct tcttcagaca
     7561 tacggtttgc aatatcttcc atgatggtat ttttacgctg accggacttg taggcattca
     7621 tctgttcaac aatatatgcc tgatgctgac cgcccaaacg cggataagcc tgaatttcgc
     7681 ttccgcctcc cggcataccc gcaccgctcg gaccgtggca ggacatacac gccggcactt
     7741 ttttatcgct caaaccgccg cgatagattt tcgcacccaa ttcgggattt tccttaggat
     7801 tggcttcacc ggatttgggc tgctgtttgg catagaatgc ggatacgttc aaaatatcct
     7861 gatcgctcaa attcattacc accggtttca tcacagctgc cgaaccgtgg gtgcgtttgc
     7921 cgtcgcggat gccgatagtt tgatgataga tgtaagcagt atgctgtgcc gccaaacgcg
     7981 gatacatcgc aatgccgctg ttaccgtctg ctgcatggca agccgcacaa accgttgcgg
     8041 caacctgttt gcctttttcc acgtctgctt tgggagaggc ggaaaccgca ccggcagcca
     8101 aaacaaaggc caataaagtc aatcgtttca tggagtgctc ctgattacag cattggataa
     8161 cgcaacaatg ctctttttat attcaaatac gggatttttg acccgattaa aaccgatgat
     8221 tctgtaaacg tgttattcta tactaaattt acattaaatt accactgtgt ttcacataaa
     8281 accaaccgca tatttttgct gtcggacaaa cggcggcgga aaacaaggat atgcccatga
     8341 acctttttca aaacgccaaa ttcttcacga cggtcaacca ccttaaagac ctgcccgaca
     8401 cccctctcga aattgccttt gtcggcagga gcaatgccgg aaaatccagt gccatcaata
     8461 ccctgaccaa ccatgtccgt cttgcctacg tttcaaaaac acccggacgg acgcagcata
     8521 tcaacttctt cgagctgcag aacggcaatt ttatggtcga tttgcccggc tacggttatg
     8581 cccaagtccc tgaagcagta cgcgcacatt gggtcaatct gctcggcgac tatctgcaac
     8641 agcgcaaaca gcttatcggg ctggttttga ttatggatgc ccgccatcct ttaaaagaac
     8701 tcgacatccg tatgctggat tttttccaca cgaccggcag accggttcac atcctgctgt
     8761 caaaagccga caaattatcc aaaaacgaac agataaaaac cctgtcccaa gtcaaaaaac
     8821 tgctcaaacc ttattccgac aggcaaaaca tcagcgtaca gctgttttcc agcctgaaaa
     8881 aacaaggtat tgacgaggcc aaccgaactg tcggaagctg gtttgacgca gcagatgctg
     8941 ccgcttcctc tccagaggaa aactgacccc aattatacgg aaaccgtatt tcccccactt
     9001 gaccgaccgc aaacatttaa aaaattgcca ctgccaaatc taaaatgccg tctgaaagac
     9061 ttttcagacg gcattttgcg gagtctttaa aacagagaat ccaactgctg ctgtttggaa
     9121 ccagtattac tcggaagcac cggcgtttcc tgcatatctt ggcggacttc gtcatccgcc
     9181 gcctgccgtc cgccttctgc cgcgcccccg tcatcttctt ttgcccgtcg ggaaggttgc
     9241 ggcgcaatac cgctgttgtc cagcgtcaag cccggatcgg ttaccatacg ttccttcata
     9301 tagtattcgc cattgctgct gaccacacct tcaggcattt tcatcccctt gccctgcttt
     9361 cctttcaacg caaaacgcat atagtccacc caaaccggca ccgcaatcgt accgccgtag
     9421 ccgacacgcc ccatactctt aggtttgtcg aagccgatat atacggcagt aaccacatca
     9481 gggttaaaac cgacaaacca cgcatcctta ttgtcattgg tcgtacccgt tttaccggca
     9541 atatccgttc ttcccaacgc agctgccccc cttgccgtac cgacacggac cacatcctgc
     9601 ataatcttat acataatata ggcattgcgc ggatcgattg cctgaggcgc attttgccca
     9661 gccaccaaag gttgcatttg ggcgcgcaac ctgccgtctc tgtcataaat cttatcgatt
     9721 acgtgcgaag aaaccctata tccgccgttc gcaaatacgc tatatgcctc cgccactttc
     9781 aacggcgttg tctcgcccgt acctaaagcc atagacaggc ttgccggcag ctcggacgac
     9841 ctgaagccga aacgccggat atactgttgc gcgtaaccga caccgataga catcaaaata
     9901 cggatggaaa ccatattctt ggaagccgtc agagcctgtc tcaaagtaat gtagccggaa
     9961 tatctgccgt ctgaattttt aggtgtccaa accgaaccgt tcggcccttt ccccggcagg
    10021 gaaatcggcg catcgttaac cactgtggac gcggtcatcc ccttagataa tgccgccgaa
    10081 tagacaaacg gcttaaaggt cgaacccggc tgccgcattg cctgaacggc acgattgaat
    10141 gttttgctgt gaaaatcata accgccgacc agcgcgcgca cagctccggt ttttgcatcc
    10201 agcgaaacca aagccccctg cagcaacggc tcttgaacca ccgcccaacg cccgccgttg
    10261 tttttgacac ggatgaccgc gcccctgcgg atacggtcct cccccatttt ttcattattg
    10321 accgcgcggg ccgcaaagcc caaggcgcgc ctatcaagcg taacccgcct gccgccgggc
    10381 agctgtatga cgacattttt ctttttagtc acatccaaca caacggcggg aaccatttta
    10441 tcgacggtat agagtcccga cagatactgg ctgacagtct cctcgacatc ttcactctta
    10501 ctcaaatcga tatagttttc cgcaccgcgg tagctgctgc cgcgatcgaa attccgtaga
    10561 gccttgcgca atgcctcggt tgccaccttc tgatgatcgg cgcggaccgt ggtataaacc
    10621 ttaaaaccct gcgtataggc atcttcaccg tatttctcat acagttcctg acgcaccatt
    10681 tccgccacat ataaagcact ctgatcgatt ttccgaacaa accgctcgta atgcagttcc
    10741 tcattcaacg cctgatcgcg ctgttgcacg gtaatcatct tctcctcgag catattgttc
    10801 aaaatatact tctggcgcaa cttggcacgt tctggattaa caatcggatt ataggcagac
    10861 ggagccttgg gcagtcccgc aagcatggcg gcttccgcca aagtcaaatc tcggacattc
    10921 ttattgaaat agatttgcgc ggcagatgca aaaccatagg cgcgctgacc gaggtaaatc
    10981 tgattgaaat acaactcgag gattttgtct ttgcttaaag actgctcgat tttataggca
    11041 agcaacacct cattgaattt gcgtgtgaac gttttttcac tgctcaaata aaaatttttc
    11101 gccacctgct gcgtaatcgt actcgcaccc gactgcacgc tgccggacac gacattgccg
    11161 acggcagcgc gggcaacacc ccaaacatcc accccccaat gccggtaaaa gcgtttgtcc
    11221 tcggcggcga taaccgcatt ccgcaacacc tctgggaaat cgccgatttt tgtaaattcg
    11281 cgccgctgct ccccatacat accgatgact tccccatccg ccgaataaat agtcaacggc
    11341 attttaggct ggtaatgctg caaagaatcc aaagacggca gtttcggata cgttaccaaa
    11401 atagcaatgg caaccaaacc cactccaaat acacaaaacc caaaaaccaa accaaaacaa
    11461 gtcgttaaaa tctttttaat catagctgaa taataattta ccattattgg tattaaataa
    11521 agtaaaatag caaccgattt ctacaaagca cggtttcaat gtgcaaagaa caaggaatcc
    11581 attacggata ccgaaacggt tactcactgt acaaataaag caggaacttt catcatgcgc
    11641 ttgtttaaaa gcttgaaaaa ccctaaaaaa acagatgcca agctccctaa aaaatcttcg
    11701 ggactcaata accgcgcggc aatcggcatc gatatcgacc agcattccat caaaatggtc
    11761 caattgtcag gacgtagttt aaaccaaatt caattggaaa aatacgtcat tgccaaatta
    11821 ccaaagaata tcattcaagg caataaagtc caaaattacg atcaacttgt tacatatttg
    11881 caacaagcct atgccaaact gggtacttcg tgcaaaaaca tcatcgcgtc cgtcccgcaa
    11941 aatctggcaa ccatcgaaca attgacctac acagacaaag atgcagaatt agacctgcag
    12001 gggttcgtgg agtcctccat ctccgaagtc agctcgatat cgctcgaaga agccaattac
    12061 gactatcagg tcttgtccca atcggccgcc ggcgaagctg tgttggccgt cgcatcgaga
    12121 aaggatgaaa tcgaacccct gattgacgca ttcaacgccg ccggtatgaa attatccgcg
    12181 cttgatgtgg acattttcgg acaatacaac gcctacgcgc tatggataaa ccatttcgcc
    12241 cccgagcttg cagccgaaaa agtcgccatt ttcggcgtat atgccgcaca gacctacgcc
    12301 ttggtcatcc aagacggaaa aatcctatac aaacaggaaa cctccgtcag cgaagaacag
    12361 ctcaaccaac tcatccagcg cacctatcag gtaacagaag aaaaagcgga agaaatcatc
    12421 aactccccgc aaaaaccttc cgattaccaa gaaagcgtgg caaactattt caaccagcag
    12481 attacccaag aaatacaaag ggtcttgcag ttttattaca ccacgcagac cgcagacgat
    12541 atgaccgaca tcaagcatat cctgctgacc ggggaagcgg cgcgccagga aggcatcgcc
    12601 caaaccgtcg cctcacaaac caatgcagat gtacaatgcg tccatcccgc gcgttatttt
    12661 gcggacaacc tcaaaacaga caaacaacaa ttcgaacttg atgcgccgac actgaccagg
    12721 gcgttcggtt tggcggtacg gggattataa ttatgaacaa tttaatcaaa atcaacctcc
    12781 tcccctacag ggaagagatg aacaagcgca aacagcagca gtttaaaacg ctgatgtacg
    12841 gtgccgtgct gacgggcgtt gccgccgttg cggcaaccta cctgtttatc gacaatatga
    12901 tcaataacca gtcggaaaga aacacgctgc tggaaacctc catcgcacac ttggataccg
    12961 agctgtcgga aatacaaaag ctcaaacagg aaaaagatgc cttcctgatt aagaaaaaca
    13021 aaatcgagga gctccagctc aaacgcctcc aagccgcaaa aatcctcgac agcctgaatg
    13081 aggccgtccc cggaagcacc tacctgacct cgctggatgc cgttaccgcc gactcttatc
    13141 ggctcagcgg caggacatcc agcgacaacc gcgttgccgc catgatgagg gcgatgccca
    13201 ataccggcat attcaagcaa cccgaattgt taagcatcaa gaaaaacaat tcgcatcaag
    13261 aatttaccct tcaggcaaca ttacaaccca tcgtaaaggc ggccgaatcc aaagagaatc
    13321 cggcttcggg aaacgcacag gaggcaaact gaatggcttc taaatcatct aaaaccaact
    13381 tggatctcaa caaccttcac ctgctcaacc ttcctgccag gctttttatc gccctgctgg
    13441 ccgttgccgc cgtgctgggg ctcggttatg ccggattgtt caaaagccag atggaatccc
    13501 ttgaggaata cgaagcaaaa gaaaccgaac tgaaaaacac ctacaaacag aaaagtatcg
    13561 acgcggccag cctgaacaac ctgagggacg aacttgcctc aatccgctct gccttcgata
    13621 tcatgttgaa acagctgccg acagatgcag aaattcccaa cctggttcaa gagcttcatc
    13681 aggcaggttc gagcaacggc ctgcgcttgg acagcgttat gccccaacct cccgtagatg
    13741 acggccccat caaaagatta ccctattcca tttccattac cggaaattac gaacagatca
    13801 gccaatttac ccgcgatgtc ggcagcctct cccgaatcat tacccttgag tcgctgaaaa
    13861 tcgcccaatc tccggaaaac ggcggcaatc ctgacggcaa gagcagcatc ctgaacctca
    13921 gcgccattgc caccacctac caagcaaaat ccgtagaaga gcttgccgca gaagcggcac
    13981 aaaatgccga gcaaaaataa cttacgttag ggaaaccatg aaacactatg ccttactcat
    14041 cagctttttg gctctctccg cgtgttccca aggttctgag gacctaaacg aatggatggc
    14101 acaaacgcga cgcgaagcca aagcagaaat catacctttc caagcaccta ccctgccggt
    14161 tgcgccggta tacagcccgc cgcagcttac agggccgaac gcattcgact tccgccgcat
    14221 ggaaaccgac aaaaaagggg aaaatgcccc cgacaccaag cgtattaaag aaacgctgga
    14281 aaaattcagt ttggaaaata tgcgttatgt cggcattttg aagtccggac agaaagtctc
    14341 cggcttcatc gaggctgaag gttatgtcta cactgtcggt gtcggcaact atttgggaca
    14401 aaactacggt agaatcgaaa gcattaccga cgacagcatc gtcctgaacg agctgataga
    14461 agacagcacg ggcaactggg tttcccgtaa agcagaactg ctgttgaatt cttccgacaa
    14521 aaacaccgaa caagcggcag cacctgccgc agaacaaaat taagaagagg attactccat
    14581 tatgaatacc aaactgacaa aaatcatttc tggtctcttt gtcgcaaccg ccgcctttca
    14641 gacagcatcg gcaggaaaca ttacagacat caaagtttcc tccctgccca acaaacagaa
    14701 aatcgtcaaa gtcagctttg acaaagagat tgtcaacccg accggcttcg taacctcctc
    14761 accggcccgc atcgccttgg actttgaaca aaccggcatt tccatggatc aacaggtact
    14821 cgaatatgcc gatcctctgt tgagcaaaat cagtgccgca caaaacagca gccgtgcgcg
    14881 tctggttctg aatctgaaca aaccgggcca atacaatacc gaagtacgcg ggaacaaagt
    14941 ttggatattc attaacgaat cggacgatac cgtgtccgcc cccgcacgcc ccgccgtaaa
    15001 agccgcgcct gccgcaccgg caaaacaaca ggctgccgca ccgtctacca agtccgcagt
    15061 atccgtatcc gaacccttta ccccggcaaa acaacaggct gccgcaccgt ttaccgagtc
    15121 cgtagtatcc gtatccgcac cgttcagccc ggcaaaacaa caggcggcgg catcagcaaa
    15181 acaacaggcg gcagcaccag caaaacaaca ggcggcggca ccggcaaaac aaaccaatat
    15241 cgatttccgc aaagacggca aaaatgccgg cattatcgaa ttggcggcat tgggctttgc
    15301 cgggcagccc gacatcagcc aacagcacga ccacatcatc gttacgctga aaaaccatac
    15361 cctgccgacc acgctccaac gcagtttgga tgtggcagac tttaaaacac cggttcaaaa
    15421 ggttacgctg aaacgcctca ataacgacac ccagctgatt atcacaacag ccggcaactg
    15481 ggaactcgtc aacaaatccg ccgcgcccgg atactttacc ttccaagtcc tgccgaaaaa
    15541 acaaaacctc gagtcaggcg gcgtgaacaa tgcgcccaaa accttcacag gccggaaaat
    15601 ctcccttgac ttccaagatg tcgaaatccg caccatcctg cagattttgg caaaagaatc
    15661 cgggatgaac attgttgcca gcgactccgt caacggcaaa atgaccctct ccctcaaaga
    15721 cgtaccttgg gatcaggctt tggatttggt tatgcaggca cgcaacctcg atatgcgcca
    15781 acaagggaac atcgtcaaca tcgcgccccg cgacgagctg cttgccaaag acaaagcctt
    15841 cttacaggcg gaaaaagaca ttgccgatct gggcgcgctg tattcacaaa acttccaatt
    15901 gaaatacaaa aatgtggaag aattccgcag catcctgcgt ttggacaatg ccgacacaac
    15961 cggaaaccgc aatacgcttg tcagcggcag gggcagcgtg ctgatcgatc ccgccaccaa
    16021 taccctgatt gttaccgata cccgcagcgt catcgaaaaa ttccgcaaac tgattgacga
    16081 attggacgta cccgcgcaac aagtgatgat tgaggcgcgt atcgtcgaag cggcagacgg
    16141 cttctcgcgc gatttgggcg ttaaattcgg cgcgacaggc aagaaaaagc tgaaaaatga
    16201 tacaagcgca ttcggctggg gggtaaactc cggcttcggc ggcgacgata aatggggggc
    16261 cgaaaccaaa atcaacctgc cgattaccgc tgccgcaaac agcatttcgc tggtgcgcgc
    16321 gatttcctcc ggtgccttga atttggaatt gtccgcatcc gaatcgcttt caaaaaccaa
    16381 aacgcttgcc aatccgcgcg tgctgaccca aaaccgcaaa gaggccaaaa tcgaatccgg
    16441 ttacgaaatt cctttcaccg taacctcaat cgcgaacggc ggcagcagca cgaacacgga
    16501 actcaaaaaa gccgtcttgg ggctgaccgt tacgccgaac atcacgcccg acggccaaat
    16561 cattatgacc gtcaaaatca acaaggactc gcctgcgcaa tgtgcctccg gtaatcagac
    16621 gatcctgtgt atttcgacca aaaacctgaa tacgcaggct atggttgaaa acggcggcac
    16681 attgattgtc ggcggtattt atgaagaaga caacggcaat acgctgacca aagtccccct
    16741 gttgggcgac atccccgtta tcggcaacct ctttaaaaca cgcgggaaaa aaaccgaccg
    16801 ccgcgaactg ctgattttca ttaccccgag gattatgggt acggccggca acagcctgcg
    16861 ctattgatgc gtcaaaataa gggcatatgt tttacggcat atgccctttc tttatgcttt
    16921 ttgccgcgac cgaaatgccg tcattcccgc gcaggcggga atccagtccg ttcagtttcg
    16981 gtcagtttcg gtcatttccg ataaattcct gttgcttttc atttctggat tcccactttt
    17041 gtgggaatga cggcggaagg ggtaaatcct cacaacccaa agcctcgtca tttccacaaa
    17101 aaacagcaac ccgaaacagc aacttaaaac cccgtcattc ccgcgcaggc gggaatctag
    17161 gtctgtcggt tcaggaactt atcggataaa acggtttctc caaccctgcg ttctagattc
    17221 ccactttcgt gggaatgacg ggatatgggt ttccgtgcgg acgtgttcgg atttccgcct
    17281 gcgcgggaat gacggcgaca gatgcccaac ggtctttata gtggattaac aaaaatcagg
    17341 acaaggcgac gaagccgcag acagtacaaa tagtacggaa ccgattcact tggtgtttca
    17401 gcaccttaga gaatcgttct ctttgagcca aggcgaggca acgccgtact ggtttttgtt
    17461 aatccactat agtattgata aacatattat cttcaatata ttcaattgga taattgttta
    17521 cctaagcaaa gataattgcc ttttcctgac aaataagtga aatcaacgga ttgtcaaaac
    17581 acagcctgaa ataaaaaacc tccctgattt cttttatttg tccttaaaat cagaaaggtt
    17641 cgggatggtc gggttatttt tccaaacgta ccgccgccct gccgatttcg tataaaattc
    17701 cgccgtaacc cgacaagccc gaaccctgtc gccccgaaag gcggggtgtc aaacattaag
    17761 gaattgtgat gaaaaacttt aacggcaaac tcatcctcat cggactgatg ggcgcgggca
    17821 aaaccacgct gggccggcaa atggcgcagc ggctggatta ccgtttttac gacagcgatc
    17881 acgaaatcgc cgcagccgcc ggcgttccca tccccaccat atttgaaatg gaaggcgaac
    17941 agggattccg ttcgcgcgaa accgccatac tcaaaaagct ggttatcctg ccccatatcg
    18001 tcctgtccac cggcggcggc gcggtgttaa aagaagaaaa ccgcgccctt atccgcaaaa
    18061 gcggcacggt cgtctatctg cacgccccgc ccgaaaccct gctcgaacgc acgcgctgcg
    18121 acaacagccg tcctttgctg caagttgccg atcctttggc gaaattacgt gaactctacg
    18181 ccgcacgcga ccccgtttac cgccaaaccg ccgactttac cgtagaatcg gcaaactgcc
    18241 gggaaaccgt gcaaaccctg ctcaaacgct tatcccgata aaccggcata tgcgccgcgc
    18301 ccagaaaacc aaaccgcgcc ccgcccggcg ggccggcggt tcaaacttta aggaacaaca
    18361 atgaaaacac tgaccgtaca caccccgtcc cacagctacc ccatctttat cggcaacgga
    18421 ctgctgccgc aggcaggaag cctgctcaaa ccgcatttgg gcaaacgcgc cgccatcatc
    18481 gccaacgaaa ccgtcgcccc gctctacctc ggcacgcttc agacggcatt ggatgcggca
    18541 ggcgtatccc atttcagcat catcctgccc gacggcgagg cgcacaaaaa ctggcagacg
    18601 ctcaacctca tctttgacgg gctgatgcaa aaccgcgccg aacgcaaaac cacattaatc
    18661 gcactgggcg gcggcgtgat cggcgacatg gtcggctttg ccgctgccac ctaccagcgc
    18721 ggcgcaccgt tcgttcaaat accgaccacg ctgttgagtc aggtcgactc atcggtgggc
    18781 ggcaaaaccg ccatcaacca cccgctcggc aaaaatatga ttggcgcgtt ttaccagccg
    18841 caggcggtgc ttgccgactt ggacacgctg cacaccctgc ccgcccgcga attgtccgcc
    18901 ggtatggcgg aagtcatcaa atacggcgcg ctcggcgaca tcggcttttt tgaatggctg
    18961 gaacagcata tgcccgaact gatgacgctc gatcgggaaa aactcgccca agccgtgtac
    19021 cgctgctgcc aaatgaaggc agacatcgtc gcccaagacg aaaccgaaca gggcatacgc
    19081 gcatggctca acctcggaca caccttcgga cacgccattg aaaccgagat gggttacggc
    19141 acttggctgc atggagaagc catcgccgcc ggctgcgtgt tggcggcgcg tttgtccgaa
    19201 caactgggca aaacctccgc cgcagatacc gcgcggctcg ccgccctgct cgaagccgcc
    19261 ggactgccgt ccgcgccacc cgtgtttgcc tttgaaaaat ggctggaaca catgagccac
    19321 gataaaaaag tcagcggcgg catcatgcgc tttatcggtc tgaaccggct gggcgaagcc
    19381 aacatcaccg aaattaccga cacggacatc ctccgccgca ccctgcaacc gtatctctga
    19441 tttcctctgc cgatgtgctg ccgcgcgggt ttgacgcacg atgatgattt tccatcatct
    19501 ttctccgcaa aagcgggaat ccagtccgtt cggtttcggt cgtttccgat aagttcccgt
    19561 tgcttttcat ttctag
//
LOCUS       DAWHRA010000024        19541 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWHRA010000024 DAWHRA010000000
VERSION     DAWHRA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.024"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(233..742)
                     /locus_tag="WIB30_000627"
     CDS             complement(233..742)
                     /locus_tag="WIB30_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ3796739.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(1074..1406)
                     /locus_tag="WIB30_000628"
     CDS             complement(1074..1406)
                     /locus_tag="WIB30_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ3796740.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDMPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            complement(1451..1558)
                     /locus_tag="WIB30_000629"
                     /pseudo
     CDS             complement(1451..1558)
                     /locus_tag="WIB30_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1697..2533
                     /gene="cysT"
                     /locus_tag="WIB30_000630"
     CDS             1697..2533
                     /gene="cysT"
                     /locus_tag="WIB30_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ3796741.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            2788..3648
                     /gene="cysW"
                     /locus_tag="WIB30_000631"
     CDS             2788..3648
                     /gene="cysW"
                     /locus_tag="WIB30_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ3796742.1"
                     /translation="MKPYSANPNLTEPRWLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            3645..4718
                     /locus_tag="WIB30_000632"
     CDS             3645..4718
                     /locus_tag="WIB30_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ3796743.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(4775..6301)
                     /gene="ilvA"
                     /locus_tag="WIB30_000633"
     CDS             complement(4775..6301)
                     /gene="ilvA"
                     /locus_tag="WIB30_000633"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ3796744.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            6449..7618
                     /locus_tag="WIB30_000634"
     CDS             6449..7618
                     /locus_tag="WIB30_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ3796745.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVEKRSRWQRLWACLTGQ"
     gene            complement(7743..8315)
                     /locus_tag="WIB30_000635"
     CDS             complement(7743..8315)
                     /locus_tag="WIB30_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ3796746.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(8382..9365)
                     /locus_tag="WIB30_000636"
     CDS             complement(8382..9365)
                     /locus_tag="WIB30_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ3796747.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(9532..9607)
                     /locus_tag="WIB30_000637"
     tRNA            complement(9532..9607)
                     /locus_tag="WIB30_000637"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9572..9574),aa:Gln,seq:ttg)
     gene            complement(9616..9691)
                     /locus_tag="WIB30_000638"
     tRNA            complement(9616..9691)
                     /locus_tag="WIB30_000638"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9656..9658),aa:Gln,seq:ttg)
     gene            complement(9704..9779)
                     /locus_tag="WIB30_000639"
     tRNA            complement(9704..9779)
                     /locus_tag="WIB30_000639"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9744..9746),aa:Gln,seq:ttg)
     gene            complement(9787..10632)
                     /gene="ispE"
                     /locus_tag="WIB30_000640"
     CDS             complement(9787..10632)
                     /gene="ispE"
                     /locus_tag="WIB30_000640"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ3796748.1"
                     /translation="MNIADGRQAFSAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHESLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            complement(10642..11223)
                     /locus_tag="WIB30_000641"
     CDS             complement(10642..11223)
                     /locus_tag="WIB30_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ3796749.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            complement(11363..13207)
                     /locus_tag="WIB30_000642"
     CDS             complement(11363..13207)
                     /locus_tag="WIB30_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796750.1"
                     /translation="MLPNRFKMLTVLTATLIAGQVSAAGGGAGDMKQPKEVGKVFRKQ
                     QRYSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPE
                     VAERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLE
                     EVLAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSV
                     QGREKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQ
                     EMEIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAY
                     GRGTEEQRSRAALTAAMMYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGG
                     RAALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGS
                     NTELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLTDSKRLD
                     EGFALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGE
                     VLWALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            complement(13372..14208)
                     /locus_tag="WIB30_000643"
     CDS             complement(13372..14208)
                     /locus_tag="WIB30_000643"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ3796751.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGIAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFHIVSPDIACFGKKDYQQLAVIKGFVED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLVQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRADTLEVARAGDKKLVVLAAACLGTTRLIDNLEIK
                     LP"
     gene            complement(14331..15122)
                     /gene="panB"
                     /locus_tag="WIB30_000644"
     CDS             complement(14331..15122)
                     /gene="panB"
                     /locus_tag="WIB30_000644"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ3796752.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVLAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            complement(15264..15339)
                     /locus_tag="WIB30_000645"
     tRNA            complement(15264..15339)
                     /locus_tag="WIB30_000645"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15304..15306),aa:Thr,seq:tgt)
     gene            complement(15374..15449)
                     /locus_tag="WIB30_000646"
     tRNA            complement(15374..15449)
                     /locus_tag="WIB30_000646"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15414..15416),aa:Thr,seq:tgt)
     gene            15615..16406
                     /locus_tag="WIB30_000647"
     CDS             15615..16406
                     /locus_tag="WIB30_000647"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ3796753.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            16461..17102
                     /locus_tag="WIB30_000648"
     CDS             16461..17102
                     /locus_tag="WIB30_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ3796754.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(17180..18103)
                     /locus_tag="WIB30_000649"
     CDS             complement(17180..18103)
                     /locus_tag="WIB30_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ3796755.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            18267..19136
                     /locus_tag="WIB30_000650"
     CDS             18267..19136
                     /locus_tag="WIB30_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ3796756.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            19179..19367
                     /locus_tag="WIB30_000651"
                     /pseudo
     CDS             19179..19367
                     /locus_tag="WIB30_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4838 a         5519 c         4594 g         4590 t
ORIGIN      
        1 aagtggcggg aatgacggga tgtaggtttt cttaaccctg cgtcctagat tcccgctttc
       61 gcgggaatga cgaaaagtgg cgggaatgac ggttcgggca ttccttaaat cacccgtgta
      121 tcgctgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccag tacggcaagg
      181 cgaggcaacg ccgtactggt ttttgttaat ccgctataat tgatgaaacg ggttaaaaaa
      241 gtgttgccat cgcctgttct tcttgtcgga tacgcttaaa taagaccagc aaataaatgg
      301 gcaggccaat caataaaaca taccacgctt gacataataa ggcgatgcca atcagttcgg
      361 gtatgatgtt taaaaaataa ttggggtggc ggaatgtttt aaacaaccac gaacgattaa
      421 tttgatgatt tggtaaaata tagattttaa ccgtccaaat ctcccccaac tgcttaataa
      481 tcaatgacaa tatcacaaac gaagccatca ccgtcagcgt accaatcaag gatatgccat
      541 taaaagcagt gtcagaaagc catacccaaa caaaacacgc caaataataa agcgtatgaa
      601 ctgccgcaag cagcgtggaa ttggtttttc cgtattgttt tgcccctttg gcaatcaagg
      661 ctttttcatg tttaatagag acggctaaaa ataacagtct gatgataaaa aacaggctta
      721 aaatgcttaa aatcattgtc attgatgttt ttccattgaa attgaaataa atataaatcg
      781 gattaatggt atttttaatt aatgatgttt cagaccatca tgctctataa acaattccca
      841 ttaagtccgc gccgcaacct gctataataa gtctgcaatc ggcgcaaatc aatgctttgc
      901 gtttattgcc atcccaaaat aattgatgct gccttaatta taataccaag ataagttttt
      961 ttattcaata aaatacaaag ggaagcgttc agcccattgc aaacagatgc aatccaccga
     1021 ttatttaaaa aacggcaaag ccttgccccc cttgcggcaa gcctgcaatg cctttaatgt
     1081 ccgcaggcgc aagcgtcgcc gtggtgcccg caaccctgtg ccgtttcgag ccattcgtcg
     1141 tcatcgccgt caaactcttc cacttcgtca aactcgccgc gttccaccat gacgcacaac
     1201 tcttcaaggc tttccgcgcc ttccacccaa aaacagggaa tgcccggcgg catatcgggc
     1261 gcaagcagtg ccgctacgcc ttcatgccac gcaatccaat agccgttgtc cgtacggacg
     1321 aattgtgcgt tttcgttgac cgactcgcct tccgtacctt ccagcatacg ttccgcaatg
     1381 tccggttgaa aagataaaat ctgcataagt gttcctttat atgatggttt tccgtcaaaa
     1441 caaggtgtta tagtggatta aatttaaacc agtacggcgt tgcctcgcct tgccgtacta
     1501 tttgtactgt ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctatatttta
     1561 aaacatcgcg cccgcttgag aaactgccaa ccgctttata acaaattcgt ctttgcacca
     1621 aactttccat tctttccgtt tttcggacgg catcgttaaa gtagtccttc cttttcctta
     1681 ttttcagcat tgttttatgt taccgctcaa aacgcccggc gtactgcccg gcttcaaact
     1741 cagccttggt ctgaccgtat tgtgcctgtc gctgcttgtg gtcttgccgt ttgcgatgat
     1801 ggcggcgaag gcggcggaaa tcggctgggg cggcttttgg aacacgattg ccgagccgaa
     1861 cgtgttggcg gcggtgtggc tgagtttgcg gatgtcgttt tatgcgatgc tgaccaatgt
     1921 cgtgttcggc acgctggtgg cgtgggtatt ggtgcgctat gaatttccgg gcaagggctt
     1981 ggcgaacgcg ctggttgatt tgccgtttgc gctgccgacg gcggttacgg gtatcgcgtt
     2041 ggcaaccctg tatgcgccta acggttggat aggccgtttt ttcgagcctt tgggcatcaa
     2101 aatcgcgttt acgcccgtcg gcatttggat tgcgctggtc gtcgtcagcc tgccctttat
     2161 cgtccgcgcc gtgcagccgg tgttggagga attatcgggc gaatatgagg aagcggcggc
     2221 gactttgggc gcaaaccgct ttacgacgtt ccgccgcgtg ctgctgccgg aaatcacgcc
     2281 ggcactcttg accggcgcgg gaatgatgtt cgcgcgggca acgggggaat acggctcggt
     2341 gatttttatc gcgggcaata ttccgatggt ttctgaaatc ctgccgctga ttattacggg
     2401 caagctggaa cagttcgacg tacagggcgc gtcggcggtg gcgttgttta tgctgctggt
     2461 ttcgtttgtg attctgtttg cgctgaacgt gatgcagtgg gcgttgggca ggcgttcggg
     2521 cgcgaagggt tgaggtcgtc tgaaatacct gttaccgtca ttcccgcgca ggcgggaatc
     2581 cattggtgaa tttcagctgc cttatttatt ttctgttttc ctgttgccct gcggtggatt
     2641 cccgcctgcg cgggaatgac ggtagctaga cgtttttatt cccttaatca ataaaaggtt
     2701 gtctgaaaac gaatccgccc cacaaaaaac ggtttttcag acggcatccg aacaccttaa
     2761 aacaaccaac cggggaacac caccgccatg aaaccctatt ccgccaatcc caacctgacc
     2821 gaaccgcgct ggctgcgcgt gttgctgatt gccgccgcgc tgggctttct gctgctgatg
     2881 ctggtcgtgc cgctcgtcgc cgtgttttac gaagccttaa aaggcggttg ggatttgtac
     2941 ctgaaatcct taaacgatcc cgaagcgtgg tctgccatca aattgacgct gattaccgcg
     3001 ctgattgtcg tccccgtcaa tgccgtcttg ggcgtggcga tggcgtggct gctgacccgt
     3061 tttgattttc gcggcaagca gttgctgact accctgctcg atttgccgtt ttccgtatcg
     3121 cccgtggtgg ccggtttaat gttcgtctta ttgttcggcg cgcacacggc attgggtggc
     3181 tggctcgaag cgcaaggcat acagattatc ttcgccatcc ccggcattat tctggcgacg
     3241 ctgttcgtta ctttcccctt tgtcgcacgc gaaatcatcc cgctgatgca ggctcagggc
     3301 gacagcgaag aacaggcggc attgatactc ggcgcaagcg gctggcagat gttttggcgc
     3361 gttaccctgc ccaacatcaa atgggcgtta ctctacggca tcatcctcac caacgcccgc
     3421 gcgatgggcg agttcggcgc ggtcagcgtg gtatcgggac acatacgcgg cgaaaccaac
     3481 accgtcccgc ttttggtcga aatcttctac aacgaataca acttcaccgg cgcattcgcc
     3541 ctctccggcg tattggcact tttggcactg gcgacgctgg cggtgcagaa catcattacc
     3601 aaattacaag acaaaaaact cgccgccgcc gaaaggaacg cagtatgagt atcaccatcc
     3661 aaaacttaaa caaacacttc ggcaattttc acgcgctgaa aaacatcaac ctcaacgtcc
     3721 ccaccggcaa actcgtttcc ctgctcggcc cgtccggctg cggcaaaacc acgcttttac
     3781 gcattatcgc cggactggaa aacgccgacg gcggcaagat tctgtttgat gggcaagacg
     3841 taaccgccaa gcacgtgcgc gagcgcaaag tcggcttcgt gttccaacac tacgcccttt
     3901 tccgccatat gaacgtgttt gacaacgttg ccttcggttt gaccgtattg cccaagtccg
     3961 aacgcccgtc caaaggacaa atccgcgcca aagtcgaaga attgctgaaa ctcgtacaac
     4021 tctcgcattt ggcaaaatcc tatccgcacc aactctccgg cgggcaacgc cagcgcatcg
     4081 ccctcgcccg tgcgcttgcg gtcgaaccga aactcctgct tttggacgaa cccttcggcg
     4141 cgttggatgc caaagtacgc aaagaattac gcacctggct gcgcgacatc catcacaacc
     4201 tgggtgtaac cagcattctg gttacgcacg accaagaaga agccctcgaa gtttccgacg
     4261 aaatcgtcgt gatgaaccac ggcaaaatcg aacaaaccgg cagcgccgaa gctatttacc
     4321 gcaaacccga aaatgccttc gttaccgagt tcctcggcga aaccgacgct tttgaaggac
     4381 gcatcgaaaa aggcttctgg cattataacg gcttcgcgtg gaaattggac gcacaataca
     4441 aatggcagga acaaaccgcc accggctata tccgcccgca cgaatggcag atcgccgccg
     4501 aacacgaaac accgatgatt tgtgccgaaa tcgaaaaagt ccacgccgtc ggggcattga
     4561 cgcatatttt ggtgaaacac ggcaaacaag acgtgcatat cacgctggct ggcagcgatg
     4621 ccgcgcgtta cccaatcgcc gaaggcaaag aattgaagct gattccgaaa caggtttatg
     4681 tcttctctca aaacgaactg attgaatatt cgatttaacc atgaaagcgc aatgccgtct
     4741 gaaaggcttt cagacggcat tgtgctttca agcgtcaggc aagaaacagc ttgtacgcgg
     4801 cattttgcgt ttcctcgtga tagctgtatc ccagactttc caagaaaccg tcaaatgcgg
     4861 cggcatcgtg cggcggcaca tcgataccga ccaaaatccg cccgtaatcc gcaccgtggt
     4921 tgcggtaatg gaagagcgta atgttccaac cgccctgcat atggttcaaa aaacgcgcca
     4981 acgcccccgg acgctccgga aactcaaaac tgaccagacg ctcgttttct actttgtccg
     5041 tccgccctcc gaccatatag cggatatgga ttttggcaat ctcatcatcg gtcaggtcga
     5101 cattgggcaa tcccgcctca tccaaccggc tgccgataac cgccaaatcc tgcgggcctg
     5161 ccgcttgaag tccgacaaag atatgcgctt tttcatcgtc cccgtagcgg tagttgaact
     5221 cggtaatatt cctatttccc aatatattga caaacttaag gaagctgccg cgttcttcag
     5281 ggatggtaac ggcaaaaata ccttcgttgc cctcgcccaa ttcgctccgt tccgaaacgt
     5341 ggcgcaaacg gtgaaaattc atattcgcac cgctggtaac ggcaatcagg gtttggtttt
     5401 ccgcgccttc tcgggcgata taggctttca gacccgccaa cgccaacgcg cccgccggct
     5461 cggtaatgct gcgcgtgtca tcgaaaatat ccttgaccgc gccgcaaacc gcatcggtat
     5521 cgactgtaat gatttcatcc aaaagttctt tgcagagacg gaaggtttcg tttccgacga
     5581 ctttgaccgc cgtgccgtct gaaaacagcc cgacatcttt caaatggacg atttcacccg
     5641 cttcgaccga ctgcttcata cagcaggaat cgttggtctg aacgccgata actttgattt
     5701 cgggacggac ctgcttgata aatgccgcca cgcccgccgc caaaccgcca ccgcctatcg
     5761 gtacgaatac ggcgcggatt ggatcgggat gctggctgac aatttccatc cctaccgtcc
     5821 cttgtcctgc aatcacatca ggatcgtcaa acggtgcgat ataggtcagc ccttcctgtt
     5881 ccgccagctc catcgcataa tcgtaagcat cgttgtatga aacaccgcgc aaaaccacct
     5941 cgccgccacg gctcttaacc gcatccactt tgattttcgg cgtagtctcc ggcataacga
     6001 taacggcacg gcagcccaaa cgctgtgcgg acaatgccac gccttgagca tgattgcccg
     6061 cgcttgccgc aatcacgccg caagcgagcg catctttcgg caacttggac attttgttgt
     6121 acgcgccgcg tattttgaac gaaaaaaccg gctgcaaatc ttcgcgtttc aaaaggatgt
     6181 tgtttttcaa acgtacagaa aggctgcgtg ccggttccaa aggcgtttcg accgcgacat
     6241 catagacaga tgccgtcagg atgcggatga ggtaattgga ataaggaagg ggcgtgttca
     6301 taattcaata tgggataatc ggtttattaa aatcgcaaaa cccaaaacca tacgcccaag
     6361 acggcgcgaa agcaagaaaa atccgcccga tcagacaccc taagcgtata atcggcagac
     6421 tgaaacacgc acacaattag aatatttcat gacagcacat aaaatcctgc ccgtcctgct
     6481 ttccatcatc ttaggcgttt ctcacgcaac ggccgcatcg cccgcgccca acaaaccggc
     6541 ggtacacgcc gcccccacgt tccaaacacc cgaaaccctc acagcggcac acatcgttat
     6601 cgaccttcaa agcaaacaga ttttatccgc caaaaacatc aatacccctg tcgaaccggc
     6661 ggcactaacc caactgatga ccgcatatct ggttttcaaa aacatgaaat cgggaaatat
     6721 ccgatctgaa gaaaacttaa aaatacccga atccgcatgg gcttcagaag gaagcagaat
     6781 gtttgtacgt cccggcgata cggtcagcac cgacaaactc ttaaaaggca tgattgcact
     6841 atccgcaaac gatgccgccc taacccttgc cggccggctg ggcaacggct cgattgaaaa
     6901 ttttgtgcaa caaatgaaca aagaagcccg acgcttgggc atgaagaaca ctgtattcaa
     6961 aaatccgaca ggcttgagta gagaaggaca ggtttccacc gccaaagacc tcgcccagct
     7021 gtctgaagca ttgatgcgcg actttccgga atattacccg ctgttttcca tcaaatcttt
     7081 caaattcaaa aatatagagc aaaacaaccg caatatcctt ttatataggg acaacaatgt
     7141 aaacggtctg aaagccggac acacagaaag cggcggctac aaccttgccg tgtcatactc
     7201 cggcaacggc aggcacatcc ttgtcatcac attaggttcg gaatcggcgg aaacacgcgc
     7261 ctcagacaac agcaagctgc tgaactgggc attgcaagcc ttcgatacgc ccaaaatata
     7321 tccgaaaggc aaaaccgttg cccaaatcca aatttccgga ggcagcaaaa aaaccgtccg
     7381 cgcaggcttc ctcaaagaag cctacatcac tctgccacat aaggaagcga aaatggcaga
     7441 acagattcta gaaaccatac agccgattcc cgccccggta aaaaaagggc agattttagg
     7501 aaaaatcaaa atcaggcaaa acggatacac cattgccgaa aaagaaatcg tcgcactgga
     7561 aaatgtagaa aaaagaagcc ggtggcaaag gctttgggcg tgtctgacag ggcagtaatc
     7621 tgccgtttca aatatcccgt ttttccaaca aataaagaaa tgccgtctga aacacggttc
     7681 agacggcata aaacaacagg gcggtacgta ttgcatacgc accgccctgc tgctgaaatc
     7741 aattagcgtt tcttaccggt aacggtagca acagccagat tttcgttacg tttcagggaa
     7801 acgctttcta caccttcagg cagtttgatg tctgacaagt gcagaatgtc gccggcaacc
     7861 acttcggcac aatccaaatc caagaaagca gggatgttgg caggcaaagc aactacttca
     7921 acagaagtgt ttaacagaga tacgcggccg ccttgcagtt tgaccgcttg ggaattttca
     7981 gcgttaacga tgtgcagggg aacgcggatg cgtacgggtt ggtctgcttt cacagcttgg
     8041 aagtcgatgt gttgaacttc gcggcggaac gggtgcattt ggaaatcgcg gacgataacg
     8101 tctttggttt caccgttcag agacaactta atcaacgcag tatggaaaga ttctttttcc
     8161 aatgcgtaga atacggtttt gtgatccaca gcgattgcaa caggctcttg accttcaccg
     8221 tacagaatgc cggggatttg gccttcgcga cgcaggcggc ggctcgcacc agtgccttgt
     8281 gcttcacgaa cagaggcttg aatttcataa gtcatgttaa atactccaag ttaggtaaaa
     8341 tcgccgtcat cggccgcgac cagcttaaga cggcttcggg cttatggcag caacatgctg
     8401 cctgtcatca cttcttcatt gaaaagatat gagacggatt cttcattgct aatgcggcgg
     8461 acggtttcgg ccaacagacc ggcaatcgtt acctgacgga tacggtcgca gtttttagcc
     8521 gcttcagaca aaggaatggt atcggttacg accacctggt cgatttcgga tgaggcgata
     8581 cggctgaccg cctctccgga gaatacggcg tggctggcat aggctagaac acgttcagcc
     8641 ccccgctctt tcagggcgac ggcggctttg cacagcgtat ttgcagtgtc aatcatatcg
     8701 tccacaatca gacaggttct accttgaata tcgccgatga tgttcatgac ttccgccaca
     8761 ttggctttcg ggcggcgttt gtcgatgatt gccaagtcgg cattcaggga ttttgccacg
     8821 gcgcgggcgc ggacgacacc gccgatgtcc gggctgacga cggtcagatt ttcaatccgc
     8881 tgttgtttga tgtcgttcaa cagaatcggg gtggcataaa tattgtccac cggaatatcg
     8941 aagaaacctt gaatctggtc ggcatgcaaa tcgacagtca aaacacggtc gatccctgcc
     9001 gaatacagca tatttgccac cagtttggca gaaatcggaa cgcggacgga acgcggacgg
     9061 cggtcttggc gcgcatagcc gaaatacgga atggctgtgg taatacgacc tgccgaagca
     9121 cgcttcagtg catccgccat cgtcaggatt tccatcaggt tgtcattggt cggcgcacag
     9181 gtcggctgaa ggatgaaaac atcgcgcccg cgtacgtttt ccaacagttc gacggcaact
     9241 tcgccgtctg aaaacttgga tacggaagca ttgcccaaag aaatgtccaa atgcctgaca
     9301 acacgttgtg ccaattcggg attggcattg cctgtaaata ccatcaaact gtcgtacgca
     9361 gccatattct cacctgattt tatgtttaac ttccgctcag aaaacacaat gcttccctct
     9421 gcttgtacca agcccgacat tatacctgca acatccgaca caaacaaagg catttcaata
     9481 tttttatttc tatgaaataa aagcgtgtaa gcaggcttac acgcttttat ttggctgggg
     9541 aggaaggatt cgaaccttcg catgctggaa tcaaaatcca gtgtcttaac cgcttgacga
     9601 ctccccaaaa agggctggct ggggaggaag gattcgaacc ttcgcatgct ggaatcaaaa
     9661 tccagtgtct taaccgcttg acgactcccc aactcgtttg acttggctgg ggaggaagga
     9721 ttcgaacctt cgcatgctgg aatcaaaatc cagtgtctta accgcttgac gactccccaa
     9781 caatgtttat acggacaaca aaggatgttt tgaaagaccc tctgccaaat atgcctcgta
     9841 caaatctgaa acttgtcggt atatattgta tgcgctattc ctatcttgac acgccgtgaa
     9901 tacacacgca ccggaacctg tcattaaggc aaatccatat cgggacaact cggaataggc
     9961 tttccaaact tcagggtatt ctttaaatac aactgcctgc atatcatttc taaacggttg
    10021 cagattttgg aaagtcggca ttatgcttga ggcggaattt cgtgtcaagc tttcgtgtgt
    10081 gaaaattttt gcagtggaaa cgtggacggg cggtttgacg atgacatacc actgtttcgg
    10141 aatatccatt tcgtccagcc tgtcgcctat accccgcgca aacgcatttt tgccgaaaat
    10201 aaaaaacggt acgtccgccc ccagagccgc gcccgaatca atgagctgcc gctgcgtcag
    10261 accgcactgc caccaacggt tcaacaccag caaaacggtt gccgcatccg agcttccgcc
    10321 gcccaaaccc gcccctgtcg ggattttttt gtccagccat atttccacgc cggcggggtt
    10381 gcgcgcatat ttttgcagca acgatgcggc acggtagctc aaatctactt cctgcggcat
    10441 tccatcaacc ggattgtgca ggatgatttt gccgtcgtcc ctcggtttca aatatacggt
    10501 atcctgcaaa tctatcaggc agaatatgct ttcgatattg tgataaccgt cttcccgcct
    10561 gccggtaatc ctcaaatcga gattcagttt tgcaggtgcg gaaaacgcct gccgtccgtc
    10621 cgcaatattc atctgtccgc cttatctcgt gcgcgccgca cagcgttccg gggtttcggt
    10681 ttcagacggc ataccgattt cggtgaaaac cagcctgatg ttcaaatttc cgttattcag
    10741 ttgcaacgtt cggacttgcc ccccactgtc ggcggttctg ccgacagtcc aaccgtattg
    10801 ttccaatatg ccgtccggca ggatgcggta aggcgcgccc gccacacgcc tgccatctgc
    10861 ccagatatgc agatattgga ttggcagttt gaaaccgacc agctgcctgc tcaattcttc
    10921 cgcactttcc gcctgataga catttccttt gccgtccact gccaatgcgc cgtccctgtc
    10981 ttgacacaac tgcccgagcg tactgcccaa aggggtattg atattgatgg tttccacggg
    11041 cggttggtat gtccaatcga aatttgcata cgaacctttc ccttccgctt tcactgccaa
    11101 ccgcccttct gctgcaaaac tgctgatgtg ttcggacggc tgccacaggt tttcgttatt
    11161 ttgaggtaat tgcgcgcaag cggtcaaaag caggatgacc gatgcggata cggtgtgttt
    11221 catcagaatt tccttaacgg atgccatcct gccggccgat gggttcggcg tgccgtgcaa
    11281 aacgcgtgcg aactgcgtgc cgtttgcccg aatgccccgg cttcccggca aaccttatgc
    11341 cgtctgaaag gatggacctg cattatttcc gaggttttcg ggaaggttgg ggcaatgcga
    11401 tgccgtgacg tttgagcgtt tcccgccata ttttcttgtc tcccgtaagg tgtgccgcct
    11461 gcgtccatac gtcaaccgcc tgatcgcgtt cgcccaatgc ccacaacact tcgcccaaat
    11521 gggcggcaac ttcgggctcg gggtcgtttt caaacgaata ccgcagatac ggcagcgcgc
    11581 tttccgcgtc gcctttcagg taatacgccc agcctatgct gtcgttgaca gcggtatcgt
    11641 ccgggttgat ttggtatgcc gtctgaagca gggcgaaacc ttcgtccaaa cgtttggaat
    11701 cggtcagcag gctgtagccc agattattca taatctgagc gttatcgggt gcaagcctga
    11761 acgccctttc aagatctgaa atcatttttt tccgcttgcc aagccgatcg taaacaactg
    11821 accgctgtac caatgcctct gcctgtaact ctgtattact gccggcaggc ggtttttcga
    11881 taatcttgtc caaccccctc aaagcctccc gtttatcggg cagcttcgac agggcgagca
    11941 tctgtatttt ggacaaattg tctgccgtaa aataccgccc ctgctgttcg ggaagtttcc
    12001 gcaccctgcc gatctgccgc aaagccgccc tgccgccgtc caactcgaca gccgccgcag
    12061 ccgccagcac acctttgtcg aacaggtatt ccggcgcgga tacttttttc agccactgcc
    12121 tgactttggc gtaatccctg cggtcggcat acatcatcgc cgccgttagc gccgccctgc
    12181 tccgctgttc ctccgtcccc ctgccgtatg ccttttcggc gtagccgtcg ataacggaag
    12241 caccttcttt tcggtttgcc gccaatatcg ctgcctgaat atacaggtct gcattcggat
    12301 tgcgttccaa cagcacgttc aaacgcgcat aggcatcatc cagcctgtgc agggaaacca
    12361 gattcataat ttccatttcc tgccagacgg ccgaaaggtt ttgggtgtct gtctgctcga
    12421 aaaagccgtc gagtatttcg ggatatttgc gtgcagtcag acgcaacgtc attaaagtgg
    12481 ggggcaatat ttccgtatcg agcttcgcca aacgctgcaa agctccgatt gccttttcct
    12541 tttcccgtcc ctgtacgctg aacaccacat cggcaaccgc cgcttcgggc agatgttcat
    12601 atttcaacgc cgcgcggcga accgctttcg atgctttttg cgccaacccg tcctgttgca
    12661 cggcggcttg tgccaacaat aaaaacaccc tgcggttctg tccttcgtcc gcctgagcca
    12721 gcacttcttc cagtccgtcc agatgctgat ttcctctttc cctcagcacg ttccgcagcc
    12781 accccgcccg tttttgcgcc ttacccggta taggctcaat ctgccgccat ttctgataaa
    12841 tcatttccgc ctgttcaaac gcgttcagcg acacggccat ttccaaggcg cgttcggcga
    12901 cttcggggga ttttgtgcgt tccaacatca gcatataggt tgccagagcc gttcccgcct
    12961 gccccttttg caaggcggtt tcccctccca gcaacgtaaa tatctgatta acccgctcgc
    13021 ccactgccgc aagccgtgcg cgttcgtttt tgatttcttc ctcgctgtaa cgctgctgct
    13081 ttctgaaaac ctttccgact tccttcggct gtttcatatc ccccgcaccg cctccggcgg
    13141 cagatacctg tccggcaatc aaggttgccg tcaacacagt taacattttg aaacggttag
    13201 gtaacataat catcctttat ccgacagccg tccgaacctt cagacggcat ataatcaggc
    13261 gtaactttat cacaagcaga atcgtttgta gatttgcgaa accccgttca aaaaaataaa
    13321 tgccgtctga aacgcaaatc cgcttcagac ggcattcccg acgcttgcgg tttaagggag
    13381 ttttatttcc aaattgtcaa tcaggcgcgt cgtccccaga caggcggcgg ccaagaccac
    13441 cagtttctta tctcccgccc gcgccacttc gagcgtatcg gcgcggcgga tttcgacata
    13501 atcgaccacc cagccgtatt ctgtcaggga ttggacggca cgtttttcca aacctgcata
    13561 atccaaactg ccctgcacca aggattcggc aacagccttt aattcgcggt acaggcgcgg
    13621 tgcttcgtcg cgttccgccg cactcaaata ctggttgcgg ctcgacagtg ccaacccgtc
    13681 ttccgcgcgc cctgtatcaa caggcactat ttcaacatca aaattcaaat cttcgacaaa
    13741 acctttaatc acggcaagct gctggtaatc cttcttacca aaacaggcaa tgtccgggga
    13801 aacgatgtgg aacaatttag aaacaaccgt tgccacaccg cgaaaatgcc ccgggcggaa
    13861 tttgccgcac aactcatttt gcagattggg cggttcgacg ttgtaacgct gttccacgtt
    13921 cggatagagt tctttctcat cgggcgcgaa aacaacggca atgccttcgg cggcaagttt
    13981 gtccgcatcc tgttgcaaag tgcgcggata tttgtcgaaa tcctcgccct gaccgaattg
    14041 caggcgattg acgaaaatgc tgaccacgac actgtccgcg cgttttttcg cctcacgcac
    14101 aagcgcaaga tgtccttcat gcagattgcc catggtcggc acaaatgcca cctttcccgc
    14161 atttttacgc cacgcgcgca gttctcgaat ggtatgtatg atttgcataa cggtaatcct
    14221 tatccttcgg gcttcctgcc cgacaaaacg cgccgattat acgcgccgcg cacggcaaaa
    14281 caaaatgccg tctgaaacgg ctttcagacg gcatgctgca tgtaaaccgc tcaatctgca
    14341 aaaatatgtt ccgcagcagg gaaggttttg gctttgactt cggcaacata cgcccgaacc
    14401 gccgcttgaa cactatcatg cccctgcata aagtttttga cgaatttcgc cgtcttaccc
    14461 gggaaaatgc cgagcatatc gtgcatcacc aaaacctgcc cgtcgcaatc cgcacccgcg
    14521 ccgatgccga tggtcggaca ggaaacagtt tcagttacct tttttgccag ttccgccagt
    14581 acgcactcca tcagcacgac cgccgccccc gcatcgtcat gcgccttggc atcgttaagc
    14641 aacgcctgcg ccttgccgcc gcgcccctga actttatatc cgccgaaggc aaacacggat
    14701 tgcggggtca gaccgatgtg cgcacaaacc ggaataccgc gcatttgcaa aaattcagtc
    14761 gtttccgcca tccacacgcc gccttcgagt ttaaccatat gcgcgccggc agccatcagt
    14821 tcggcggcgg cggcaaacgc ctgctcctta ctctgctgat atgcaccaaa cggcaaatcg
    14881 ctgacaatca tcgcattttt tgcaccgcgt gctacacatt cggtgtgata acacatatcg
    14941 cgcaggctga cgggcagcgt cgatttccgc ccctgaaccg ccatccccaa agaatcgccg
    15001 accagcagca tttccacgcc ggcatcgtcc atcagcgcgg caaaactgga ttcgtaagcg
    15061 gtcagcatag cgattttttc gcccgccgcc ttcatttttt gcagtgtgtt cacagtaatc
    15121 atcagatatt gttaccccgc ccttttcaga cggccttttc agtttgaaac gggctattat
    15181 agtgaaatgc ccgcagccct gccaccgacc gtgccgcaaa tgcaaaacag cccgactgtt
    15241 ttacacaatc ggactgtcaa atctggtgcc ggcaccaaga gtcgaactcg ggaccccctg
    15301 attacaagtc aggtgctcta ccaactgagc tataccggct tactgaaatg ttttgcaaca
    15361 ccatcagcag aaatggtgcc ggcaccaaga gtcgaactcg ggaccccctg attacaagtc
    15421 aggtgctcta ccaactgagc tataccggca aagaaagcga attatgcagg caatgccgca
    15481 ccttggcaag gaatttatcc gaaagccccg gcaaatccga ataagtaatt tatttgaaaa
    15541 gaaattaatt tgaaacagaa caagcatcgg cattctgata aaatatccgc ctttcaaacc
    15601 gaccgttcac accgatggca agacatccct accgccgcct gcgtcctgca aaatccggct
    15661 ttcccgaagt cggcatttcc gaagaaggca atatccgttc gcttcacttg ggcagcgaca
    15721 ccgttcaaag ctcgatgaac ctcgaccacc cgtccgagct ggtgctctct tacagccgcg
    15781 cgatgatggg ctggctgctg tttaccgacg ctcttccgca acatatcacc caaatcggct
    15841 tgggcggcgg ctcgtttgca cgctggatag atacctacct gcccgacacg cgccaaaccg
    15901 ccgtggacat caatcctcag gtcatcgcca ttgcccgcaa cctgtttgag ttgcctttcg
    15961 agggcgagaa atttgaaatt attgaagcag acggtgcgga atatatcaaa gtcttccgcc
    16021 acaacaccga tatcatcttg gtggacgggt tcgacggcga acaaatcatc gatgcgctgg
    16081 ttgaagaacc gttcttccga gactgccgca acgcactctc ttcagacggc atattcgtta
    16141 ccaactggtg gagcggcgac aaacgctatc aacgcttcat cgaacggttg ttgagcgttt
    16201 ttgaagggcg cgtcttggaa cttcctgccg aaagccacgg caatgtcgcg gtaatggcct
    16261 tccaaagcag ccccaaagag caaaacatag acaaactcaa aaaacgtgcc gacaaactga
    16321 gcaacgcata cggattggac ttccaccgta tgcttgccgg cctgaaagcg tccaacccca
    16381 acaacggcaa gcatttccac ctttaagcaa ctatcttcac ggctgcatcg gaacatgcgg
    16441 aaaatcaaga ggaaatcatt atgctgaaac ccgaaaccat acacatatcc ggtcctgccg
    16501 gcattttaga aaccatccat atcccgtccg aacaagtacc ggcacgcggt gttgccgtca
    16561 tcaatcatcc caaccccctc cagggcggaa caaacaccaa caaagttatc caaactgccg
    16621 ccaaagcctt aagcaaactc ggcttccact gctacctgcc caaccttcgc ggcgtaggcg
    16681 gcagcgaagg cacacacgat tacggacgcg gcgaaacgca agactgcctc gccgtcatcg
    16741 actatgcccg tgcccaacat cccgaagccc ccgaatttgc cttatccggc ttctccttcg
    16801 gcggttatgt cgccacattt gccgcacaag cgcgcatacc cgatttattg ctgctcatcg
    16861 gcgcggcagt ctgccactat accggccgcc ccgagccgtc cgccgttccc aatgttgcca
    16921 aaacgctgat gatacacggc gcggaagacg aagtcgtcga aatcgggaaa gcactgaaat
    16981 gggcggaacc gcaagatttg cccgtcatta ccatcgccgg ttccacgcat ttctttcacg
    17041 gcaaactcat cgtcctgcgc gacaccatcc tccgcttcgc gcccgtatgc ctgaacggat
    17101 aaaacccctt tcagacggca tcgataatgt aaaacccgta tcggcggcat ccgccgatac
    17161 gggtttgcgt tccctctcat tataggcaaa atccgctgca aaacaccctt tcccgacccg
    17221 acaaaggatc tgcaaacgca atcttttttg ccaatagctt caaaggtttc cgataatcct
    17281 cgctgcccgc ttcagacggc acgggataga gcgcgtcatt caacagcggc atacccaaac
    17341 ccatcatatg cacgcgcaac tggtgtttct tgcccgtatg cggcgtaagg cggtaaaggc
    17401 tgaattcccc cctgttttca atcagttcga ccgtcgtatg tgcgtttggt tcgccttccg
    17461 cctcttgcgt cgtaaaaaat ttctcacccc tcaccaaacg cgaaaccaca tcgagcggat
    17521 acggcaaatc cgtccttgtc ggcgcaagcg cctcatacgt tttccatacc gttttgtttt
    17581 gaaacatcgt ctgataggct ccgcgcgtgg caggattatg cgacagcagc atcacgcctg
    17641 ccgtatcctt gtccaagcgg tgcagcggcg taatgtcctc aacattcaaa tgctgcaatt
    17701 caggccgcaa acgcaggcgc gtgagcaggg tttcccgcaa aaacctgccg ctggggatga
    17761 cgggcagaaa atgcggtttg tccaccacaa tcaaatgctc atcaatatgc aaaatctttt
    17821 cttcaaacgg aatacgcggc tcgctctcac ggctggtttc acggtaataa aacatcacct
    17881 tacccggctc gaacaaagaa tgttcgtcca acgccgcacc atccgaaccg accacaaaac
    17941 cgctgttcaa ccgcctgcgc caatcgtccg cgcccacaaa aggaaagcgg atgcacagaa
    18001 aatgcagcag cggcagcccg taaaactgct tttcatgcgg cagcaccaaa taactgggtt
    18061 tgacaccgtt caacagcgga agaggattat tgcgtttttt catgcggcgg attgtaaccg
    18121 tttttcacaa acttttcaga cggcatatcg aaacataatt gacaaaaaca aacatattgt
    18181 tttttatgat ttaaaaattt atttagacaa gattatcgga tattcattat tatcagtaaa
    18241 taagaattaa tatcaatagg agaaatatga agcgcatctt tttgcccgcc ttgcccgcca
    18301 tcctgccttt atccacttat gccgacctgc ccttgacgat tgaagacata atgaccgaca
    18361 agggaaaatg gaaactggaa acttccctta cctacctgaa cagcgaaaac aaccgcgccg
    18421 aacttgccgc accggtttac attcaaaccg gcgcaacctc gtttatcccc attccgaccg
    18481 aaatccaaga aaacggcagc aataccgata tgctcgtcgg cacgctcggt ttgcgctacg
    18541 gactgaccgg gaataccgac atttacggca gcggcagcta tctgtggcac gaagaacgca
    18601 aactcgacgg caacggcaaa acccgcaaca aacggatgtc cgacgtatcc ctcggcatca
    18661 gccacacttt ccttaaagac gacaaaaacc ccgccctaat cagctttctt gaaagcacgg
    18721 tttacgaaaa atcgcgcaac aaagcctcgt cgggaaaatc gtggctcatc ggcgccacca
    18781 cctacaaagc catagatccg attgtccttt ccctcaccgc cgcctaccgc atcaacggca
    18841 gcaaaaccct ttcagacggc atccgctaca aatcgggcaa ctacctgctg ctcaacccca
    18901 acatctcatt tgccgccaac gacagaatca gcctgaccgg aggcatccaa tggctgggca
    18961 aacagcccga ccgcatagac ggcaaaaaag aatccgcaag aaacacatcc acctacgccc
    19021 atttcggcgc aggtttcggt ttcaccaaaa ccacggcttt aaacgcatcc gcacgtttca
    19081 acgtttcagg gcaaagcagt tccgaactga aatttggcgt acagcataca ttttaagcag
    19141 gttttgattt tccaaccttg taatccaaag gagttattat gaaaaaacaa attaccgcag
    19201 ccgtaatgat gctgtctatg attgcccccg caatggcaaa cggattggac aatcaggcat
    19261 ttgaaaaata cgtattccac acgcaggcag atgcgccgat gcagttggct gagctttctc
    19321 aaaaggagat gaaggagact gagggggagt ggattcctaa tgctgttgga ggcattatgg
    19381 gaggagttgg agggcatttc agctatatgg ctagtgctat tgctagtggc tcgtataatc
    19441 gagaggcaca ttggacagca attggaggtg gtgcgttaat tggtgcgatg aaccctatta
    19501 acggaggaag agcactgatt aatggtatgc gaggtgtagc a
//
LOCUS       DAWHRA010000025        19234 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWHRA010000025 DAWHRA010000000
VERSION     DAWHRA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.025"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(126..509)
                     /locus_tag="WIB30_000652"
     CDS             complement(126..509)
                     /locus_tag="WIB30_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796757.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(514..1021)
                     /locus_tag="WIB30_000653"
                     /pseudo
     CDS             complement(514..1021)
                     /locus_tag="WIB30_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
     gene            complement(1650..2045)
                     /locus_tag="WIB30_000654"
     CDS             complement(1650..2045)
                     /locus_tag="WIB30_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796758.1"
                     /translation="MNQFCLSEYEKAFLCRIIENNMIWKEYLIEQIENSIIVSRDNTG
                     CGCYIDFQVNLERTNLNKYENIPIFFEGDITAPDKSDAATFLLYTTESLNKAGRIIDF
                     LEVAIAGTNYDEVEVYSWLKMENSLRFGL"
     gene            complement(2057..2770)
                     /locus_tag="WIB30_000655"
                     /pseudo
     CDS             complement(2057..2770)
                     /locus_tag="WIB30_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(2866..3138)
                     /locus_tag="WIB30_000656"
     CDS             complement(2866..3138)
                     /locus_tag="WIB30_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796759.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            complement(3512..4000)
                     /locus_tag="WIB30_000657"
     CDS             complement(3512..4000)
                     /locus_tag="WIB30_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796760.1"
                     /translation="MKKDIFYCEQWSYGYKKLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVHFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLESH
                     DLAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYSQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(3997..6498)
                     /locus_tag="WIB30_000658"
     CDS             complement(3997..6498)
                     /locus_tag="WIB30_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ3796761.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGNIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKSSIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGMTPAAAA
                     VVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATTGVAAGTS
                     AAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAAVTAGVLQ
                     GISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNLGDAALGA
                     IVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGKCRDGAIGAAVGEMVGE
                     TLLDGRDVGKLSPQERQKVIAYSQIIAGSVVALVKGDVNTAANAATVAVENNSLLARR
                     RVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAILRTPGEPWTRHDVQTYRQ
                     YYNQLRESRGFAVDPIYRIRINNGNEFNRIMSSKYPYNELYVANPKSATGYFRVDSYN
                     PATEEIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAVISNVPSTPTTIRGRKLEGKL
                     ILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
     gene            complement(6858..7232)
                     /locus_tag="WIB30_000659"
     CDS             complement(6858..7232)
                     /locus_tag="WIB30_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ3796762.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            complement(7251..13229)
                     /gene="hrpA"
                     /locus_tag="WIB30_000660"
     CDS             complement(7251..13229)
                     /gene="hrpA"
                     /locus_tag="WIB30_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HEZ3796763.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFVVKGSAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGEADYTGVLARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNDGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSVKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARVDAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGGYIVDIPKGNLKTEIEK
                     LAKQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGAGAAIVVIIVTALTYGYG
                     AAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYSQAAVAIINNKGDVG
                     KALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSSTGNQTIANLGGR
                     LATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASKIKTTFSDDYVAK
                     QFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATLSDAEKHKVISYSKI
                     IAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKPDKTALEKIIQSI
                     MPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKAAIS
                     TCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALSKAKAAKQAAPKETINN
                     LANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRKGAGLLDASNIPITINGKTIKPVQ
                     AISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVLPDGRLANGIISTGEWAGTKIALR
                     NFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            complement(13338..15080)
                     /gene="hrpB"
                     /locus_tag="WIB30_000661"
     CDS             complement(13338..15080)
                     /gene="hrpB"
                     /locus_tag="WIB30_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ3796764.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            complement(15141..15389)
                     /locus_tag="WIB30_000662"
     CDS             complement(15141..15389)
                     /locus_tag="WIB30_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ3796765.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTDL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGDFGEFCKGLIL"
     gene            complement(16288..16488)
                     /locus_tag="WIB30_000663"
     CDS             complement(16288..16488)
                     /locus_tag="WIB30_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796766.1"
                     /translation="MVSSDLAKSSEPVYLVKIKPDKSTITAYGEEKPLQIGMTLEADI
                     LHEKRRLYEWVLELIYSMSGKL"
     gene            complement(16440..17066)
                     /locus_tag="WIB30_000664"
     CDS             complement(16440..17066)
                     /locus_tag="WIB30_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ3796767.1"
                     /translation="MNRPKQPFFRPEVTVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITTKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRAANLFIL"
     gene            complement(17226..17486)
                     /locus_tag="WIB30_000665"
     CDS             complement(17226..17486)
                     /locus_tag="WIB30_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ3796768.1"
                     /translation="MERKNNIIELSIENLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGVGIVSGGIPAGPAIILGGTVGAAVGAVTGMGVAAYEYY"
     gene            complement(17906..19060)
                     /locus_tag="WIB30_000666"
     CDS             complement(17906..19060)
                     /locus_tag="WIB30_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ3796769.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAARNLEAASWKDINEI
                     YQRADPTAPLFYLRKKGAIVPKEEYVESFDALGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHISRQVARFAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHLA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
BASE COUNT         4803 a         4340 c         4142 g         5949 t
ORIGIN      
        1 agcacacacc ttacgcatgg attttaggtt tcatgcaggc tacagcttgc tgctgcggta
       61 taaaagtatt tttagtgatt tactgttttt ggtatttttg cccgacgggg ttaaaaatac
      121 agttgctact cttgctgcat gatattggca agatttaatt taaatgtttg ttgaatcttg
      181 tttacatcct tagcaaaaat atgatagtca ctcgaagaat agatagattt tattctatcc
      241 aaattagttt gttgataaca ttttttcatt tccgcgatga aaaaattatc ttttcctctt
      301 tcattgagac agttattttc taacagatag attgcccatg ctgtaaaaat aaatgtacta
      361 tcttccggat attcagataa ataaatttcg aattcaatta tagaatattc agctgcttca
      421 attaatgtta tatctaaatc cctgacaaat ttttccgtta tatttttaaa taatttcttg
      481 aattctatat aattttttat tttttccatg attctatttt cctgtaaaaa tatttaattg
      541 aatatttgga tagcgatttc taaactctaa aataacattg ctgcaagatt gacaggcctt
      601 taattctgta aatagatcaa ttctacctga tacattacga ttatttccga gttgctgtgc
      661 tatagtttca agtaatttat attctccatc tatatttctt aatgttcctc taggaggaga
      721 ggcaatatta tgatatttat caacagatat aggtttaaaa atttttgttt caggtaacga
      781 aataaatcca tgttcccctt tttggaaaga actaaatgct tccatccttt gcggtaatcc
      841 tttaacatca atttttgcta tcgcaagatt ccctatttgt cgatactctt ccggcagttt
      901 catgcggact ctctggcttt cagcagttaa aagcaggagc actgtcattg ctcagataac
      961 gggtaacagg tgtaggcttg gccgatattg cagcctgtgc agatacagat cctgcaacca
     1021 tcaatgtgcc attaacaacc tgaactgtac catgcagata ttctccatta gagattgctt
     1081 tcgctccctg tccaatgtta tatccccctg ctgccacagt agctgctttg acactgaagc
     1141 caagcggtcc gtttaataag taaagtgcag gttgtgcggc aacagctgat ttgtaatcaa
     1201 aagaatcata acgactcaat gtttgatagg ctttcgataa agtgttatta atatctttgg
     1261 aaggaatagt gagattaccc attgactggg gcgttttcag tatcgaagct gcccaagctg
     1321 tgttacctcc agaggcttgg ttaataaaat atgttaccta tgtatattgt ttcttcaaaa
     1381 atcaaccgta ttgtccttgg gtgattttcg taccagcaca atcagtctgc cctagttgac
     1441 agatcgcgcc acactagcca gatacagcgt atcattggta aaactgataa tgcggcaata
     1501 taaagaaaaa cttcaatagt atgttaacga aatttgacgc ataaaaatac gctaaaaaat
     1561 tttcgcttga ctaattaaaa atgggattga gcataaaatt ttaataattt atattattga
     1621 aaatatctca gattttcagt cagtttattt tacaagccga accgtaggct attttccatc
     1681 tttaaccatg aatagacctc aacttcatca taatttgtgc ctgcaatagc aacttccaga
     1741 aaatcaataa ttctacctgc tttattcaaa gactctgtag tatatagcag gaaagtcgct
     1801 gcatctgact tatccggtgc agtgatgtct ccttcaaaaa aaatagggat attttcgtat
     1861 ttatttagat tagttctttc taaatttacc tgaaaatcaa tgtagcaccc acatcctgta
     1921 ttatctctag atactattat tgaattttct atttgctcta ttaaatattc cttccatatc
     1981 atattgtttt caataattct acataagaaa gccttctcat attcagataa acaaaactgg
     2041 ttcatatatt ctacccttac attttcaaaa tagttgcaat acgagatact ccattgttga
     2101 catatacgcg aacaattacc gttataccat tgaccgttac agtaactgga taatttcctg
     2161 tttttgtcag ttttaaagca tccactgcct tttgcgtcgc tacaacagca ttttccatgg
     2221 agcctgattt ttgaatcaat gtattaccga tgcttccttg atgcccctta aagatgtgat
     2281 gaactttatt tagccaatct acaccgtatc tttcagccaa tagtcgagaa ttactttcac
     2341 ctattttttc catcgaagct aaaatccttt gagcataggc agcatcagct gttgaaaaat
     2401 ttgcttcgcc caacaaaagc cgtaattcct gaatatctcc tcgtaaaatt gcatttctta
     2461 ctcccgcttc taatgtggtt tgtggctgaa agagggaatt attctctacc gccacagtag
     2521 ccgcattcgc cgctgtattc acatccccgc ctacaacggc agacgcagtg ccggcaacca
     2581 gttttgaata ggcagtaatc ttcgctttag ctttttcgat ttcggtcgca ctcatacgac
     2641 tgaaatcagt attcttaacc aaagcctcac caacaatctc accgactgca gcgcctatcg
     2701 caccatcctg acacttaccc ttattcgccg ccgctgccgc acagcccgct atggcatggg
     2761 caatcttgtg ggtggtatat cgctgccagc cctctctgtt gataatcttg cgtatgatta
     2821 caccgtttga tggctaatcg tacctacgca ggcttaaaat ttccatcaat ccatatcatt
     2881 tgagttttca tcttcataat atggatgatc aaacaaatct ccttcccaaa tacattcact
     2941 cgttatacct tgggattcca tgaaaggaac ataagaataa aaatcttttt catattctcc
     3001 ataaaatata ttcatacctt tgagccatac tgcatcgcat tcatcacaca gaataatttc
     3061 atcatgaaga tatttaggtt tcgaatggta gagatagcct tgttgacaag caggacacat
     3121 tttcattctc ttattcatga ttactccata taatcgattt atttttgtgg gtaagcagta
     3181 gtgatttgat tgctgccttt agtctgtaca acaatacgta tatagcaacc tgagaccttt
     3241 gcaataacat aggttactaa aattttatgc tcaatctcat tttcaaaatg caaaactttt
     3301 ctgatttttc ctactttttg ctcaatatta ggaaggtttt aggcaattga aaattttttg
     3361 gcgcattttt atgcgtcaaa tttcgttaac agactatttt tgcaaaggtc tcaacctgat
     3421 aatttcctgg tttttatcag ctttatcctc agattccaaa cccaataata aaagccgttt
     3481 gaaatttttt tcagacggcc ttaaaactta attaaataat gtccggattt aacaccaaat
     3541 catattcacc aaactttgga aatagaatta ccttatcttt ttgggaatat aattctgttt
     3601 tttcatatcg ctcattatta ataaaatcct ctttcaaaac atagcaatca tccgtatttt
     3661 cattgaagaa ataagtatat ccttctgcta agtcatgaga ttccaaagtt atataatctc
     3721 tccatacagc catagaaaga aaataataat tcgaattgga atatttttga aattgatggg
     3781 ttaaataatc ccttccaaat ttatcgaaaa aatgtaccga aaaaaaacct acttcctctc
     3841 gcaaggtaat tacatattca ggttgtgtcg ccgaacctat tacggcagta tataactccc
     3901 ctttaagatg tttttcctca gcttgtttct cagaaaaagg cttatgaagt ttcttataac
     3961 cataagacca ctgctcacaa taaaaaatat ctttcttcat ttgtaaatcc ttcctgttgt
     4021 atctctaatg ataacatttt cttcttgtgc cgcccttaat acagattgtg gaattggatt
     4081 gacctgagca ggaacttcta aaataagttt tccttcaagc tttcttcctc ttatcgtagt
     4141 aggtgtactt ggaacattgg aaatgacagc accagggcta tattttctaa cagcctcctt
     4201 gatataacca atccccgtac tttcttggat ttgagaaaat tgggtaaatt ttcttgaaat
     4261 aatttcctct gtcgcaggat tatacgaatc taccctaaaa taccccgtcg ccgatttagg
     4321 attggctaca taaagctcat tataagggta ttttgatgac atgatacggt taaattcatt
     4381 gccgttgttt atcctgattc tataaattgg gtcaacagca aagcctctgg attcccttaa
     4441 ttgattataa tattgcctgt atgtttgtac atcatgtctt gtccacggct ctccaggagt
     4501 cctcagaata gcaatcccgt taaatttcgg atccagcctt cggatttgat tggtaatggc
     4561 ctggatttca agaatggcat attcatgttc caattcttgt cgcggagtcc aacgtatatt
     4621 taccctcctg cgagctaaaa gactattatt ctccactgcc acagtagccg cattcgccgc
     4681 cgtattcaca tcccctttaa ccaatgccac tacgctgcct gcgataatct gcgagtaggc
     4741 tatgactttt tggcgttctt ggggtgacag tttgcctaca tcgcgtccgt ccaacagggt
     4801 ttctcccacc atctcgccga ctgccgcgcc gattgcgccg tcccgacatt tgcctttatt
     4861 tgctaccgcc gatgcacagc ctgctacggc atgggctatc ttgtgggcaa tgtagtcttc
     4921 gctgagatta aatttgattt tgctcgctac ttctccgtgt acggtactga ctatcgcacc
     4981 cagtgcggca tcgcccaagt tgtcttttag gctgccgccg ttgatggcgg tatggacact
     5041 tgcggcagcg gtgctgttga tcaggttagc ggtcagtttg cctgctgcgg gactgtgaaa
     5101 atgtttgctg acggcttcgg ctgcttgggt gttcagcccg cttatgccct gcagtacgcc
     5161 tgcggttacg gcggcggtgg cggcctgtct gacggtgctg cttttgccca gttctttcag
     5221 ggtatggttt atgtctcctt tgttgttgat gagggaaact gcggcttggc tggcgagact
     5281 ggccagtgcg gctttgcctg cggctgtggt gatagctgca gctgatgtgc ctgctgctac
     5341 tccggttgtg gctgccgttc ctgctgctac tccagttccg gctgccgttc ctgctgcggc
     5401 tcctcccgct cctgcgcctg ccgcgcccgc cgttccggcg gctgccgggg cggacagtgc
     5461 gccgtaggtc aatacagtta cgacgataac gacgacagct gctgctgcgg gtgtcatgcc
     5521 ctcctgtttg tagtcccatt tatcgtaagc aagctgcacc tgattccagt tgatgttttt
     5581 cgcaacttga agttgtttca aataagcata ctcgggctgc ttggtgaggg tttcgatttg
     5641 ggttttcaga ttgcctttgg gaatatcgac aatgtaaccg ccgggtgcgg acagtacggg
     5701 cgcaacggga ccggtgaaac tcggcaattg caaggtttcg atgttactgc cccgtcctgc
     5761 ctgtttttgc catagagtag atttgctgct gctcacggtt tctgtgtgga tgctgctttt
     5821 gatcccttcg aggataatct tggcatctgc ccgtgcctgc tcacctacgc ctgcgcgtat
     5881 ggttgcgcca cccagtgtgg tttcaaactg tgtgccttgc agtttggtat cccagcccga
     5941 ttggaggttg gctgattctg caactaccct tgagggcagc gcggttttca tgacttgggt
     6001 ggtggtgtcg tgtgctttgc tgtagctgat gccgagaaat ctgcgccttt tttggctgtc
     6061 tagtttgtcg tagttgagct cttctacggc atagagtgtc aatttccgcc cggcttcgat
     6121 gttaatgctg cctttggggg catcgaatgc ggtggcgtag gcgtcgatgt tgccaccaga
     6181 tttgatgtcg atgccttggg atgcgctgag gtttactgcg tcgggcttgg cgtttttgtg
     6241 ttctttgact tcggtaatgt gtttgcggtt gtaccatttg ccggttttgt agctgcggcg
     6301 ttcaaaggta tagagttcgc tctgtctcgc atagtaatat tggtcaccgt aagattgaat
     6361 tttgattttg ccgttttccg aactgatatc cgtggtgctg agcaggatgc gactgttctc
     6421 attggcatac ggtgcgctaa tgctcacacc ggttttaccc gaaagttctg cagcaagcgt
     6481 agggggtgtc ttcagcacgc acgcgtctgg gcagtatata gggaatctga atatttactt
     6541 gcataacaaa tgccgtctga aaaattgtga gcttttcaga cggcattgag ccgtaaatca
     6601 tggaacgcgt gcgtgctgaa gcacacacct tacgcatgga ttttaggttt catgcaggct
     6661 acagcttgct tccataaatc atttttatca gagctcgtag gtacggttag ccgcctttag
     6721 cggcgtaacc gtacgaatga aatgccaagt tgcaaggccg tctgaaaaag ttgaaaaaca
     6781 gatttcagac ggccttgtta ttttataaag tttgctgata tgcgtacggt tacgccgcta
     6841 aaggcggcta accataccta cgcttgctca taaatatcaa tattcggcaa atcggccaaa
     6901 tctattggac acgcaatatc ccaccaaagc cattctaagt aataccaagg gtcttcaggc
     6961 catattgctt gggcatcttc caaagtaggc catatgtctt tcaatttctg cacttgttct
     7021 tttgaagttc cagttagagg aattccgata ccgtcggtat aatcatgtaa acggattgaa
     7081 ccgtcattta acagttcttc cataaaagaa cagaaattgt tttttaagtt tttatcttga
     7141 atattaatac ccatttgatt tttataagag ttaaaaattg aggataaatc gatttgaaaa
     7201 tcaagaatct taattttttg tttctcatcc acggtaaaat cctttgtaaa ttattggaat
     7261 ttaagctcca atttagtacc tttaataaat gatggaggat tctgcaaatc taatgtccat
     7321 cgtgcttgag ttgaattctc tgtttttgaa aaatttctta atgcaatttt tgttcctgcc
     7381 cattctccag tactgataat gccatttgct aatcttccgt caggcaaaac tctaaaattc
     7441 ggattctggc cagtcatctg ccgataaagc gcaaaaatct cctgttcgct tacgccgctg
     7501 taaacgggtg ctccctttaa gcttatggct tgtacaggtt tgatggtttt tccgttaatg
     7561 gttatcggaa tattacttgc atcaagcaag cctgctcctt tccttactct attgttaaat
     7621 cccgtcttcc atgcatccag ttgcgtatcg cgttgggcaa tacggaataa aatctgctgt
     7681 tctgctttgg ctaaatttgc caaattgttt attgtttctt taggagcagc ttgcttagcc
     7741 gccttcgctt tcgacaacgc tcctactgga gcttcccaag cgttgcctac cgttaccgca
     7801 cccgtggcga ttcccgcgcc cgcttcagca gcctgcgtaa ccatgacagt acaaccagaa
     7861 ggattagcca tacaggtgct gatagctgct ttaccagctg taccgatcag cggagctgtc
     7921 caacctgcag cataaactcc atagctggta atcacaatcg ggcctgtgat gccattacgg
     7981 atattgctta tccaaatggc agcatcctta tcctgcggat tagtcatcgc acctgccgca
     8041 tgtgcaggca taatactttg gatgattttt tccagtgcgg ttttgtcggg cttctgcggt
     8101 tgatgctttt tcgcattggt aggggtgctg tcaaaattca aagcattatt caccacagct
     8161 acctcagccg cattcgccgc agtattcaca tcgccgccgt tgagtgccgc cacgctgccc
     8221 gcaataatct tcgagtaact gataacttta tgcttttctg catcgctgag tgtagcaggg
     8281 tttctgccgc caagtatgga ttcagctacg atttccccaa ctgctgcgcc aattgcccca
     8341 tctttacatt ttccctgtac cagtccgcta acacaacccg ccaaagcgtg ggcgaactgt
     8401 ttggcaacat aatcgtcgct gaaggttgtt ttgattttgc tggcggcttc cccttggaag
     8461 ctattaacca atgctcccaa tgcggcattg cctaagttgt ctttcaagct gccgccgtta
     8521 acggcggtat tgataccagc tgagatacct gcattgctga ggttggtggc caatctgcct
     8581 ccaaggttgg caatggtttg attgcccgta ctgctgaaca gttcggttct taccttgctg
     8641 ttcaattggg caatatctgc tcccatctgg tttaatgcac ccgccgtcag tgcggaagta
     8701 acaatctgct tgaccgtatc actggtgccg agatctttta atgctttgcc tacatcgcct
     8761 ttattattga tgatggctac agctgcttgg ctatacaagg aggctaaagc tgcggtttgc
     8821 atggcagctg cagtagaaac agtggtagtt gctgccgctg tcgttgttgc agctgcggct
     8881 gtactacttc ctgcggcagt tacgctaccc gctgcagccg ctccgtatcc ataagttaga
     8941 gcggttacaa taatcaccac aatcgctgca ccggctccgg tcaagccttc ctgtttatag
     9001 tcccatttgt cgtaggccag ctgcacctgt ttccaatcga cgttcttggc cgtctgaagc
     9061 tgtttcaggt aggcgtattc gggctgtttg gccagctttt cgatttcggt tttcagattg
     9121 cctttcggaa tgtcgacgat atagccgccg ggtgcggaca atttgggcgg agtagggctt
     9181 tcgaagctgg gcagttttag cgtttcgata gtgctgccgc gtccggcctg tttctgccat
     9241 acggttgagt tggtttctaa tttttcttcc gactggatac ggttcacaat gcctttgagg
     9301 ataattttcg catcgacacg ggctttttcg cctacacctg cctgaatgtc ggcaccggcc
     9361 agcgtggttt tgaattcggt accttcgagc acggtatccc agcctgaacg ggtggctgca
     9421 gtttgggcga cgacgcggac aggcaatttg gtttcgttca gttcgttttt actgtaattg
     9481 ctcttaccta ccttgatgcc gataaagcgg cggctttttt ggacatccaa ctcgtgcttg
     9541 tggatgcctt cttctgccag cagttgcagc tcttcacccg caaccagggt aactttacct
     9601 gcaggggcat tgaagcgggt ggtattagct tcgatgttgc cgcctgcctg aagcgtgata
     9661 ccgttggcgg tcagctcgac gggggctggc ataatcaggt ggtcgcgggt gctggtaaac
     9721 ttggtttttc tgatgatttt gccgctttta cctttggttt ttaagaaggt ataggcatcg
     9781 ttttgtccag cctccagtac aatatcacta tgggctttga tgtctatgct gcctgaggga
     9841 gctttgattt cggatgcacc gataataata cgtgcatcat cgagtgccgc agctgcatga
     9901 atacttaccc ccgtacgtcc ggtcaaacgt gaaggcttgt tcagagcagc tttgtcgtag
     9961 tgactcttgt aggtgggctt gccaatttca tattggtcgg ttatgccgtc aatcagaata
    10021 gcagccgcct ctgaatctgc tgcctttggc aatacgcctg cggcgtgaag gttcagtttt
    10081 ttggaagcgg taatatcgga accgctgatt tcgatgcctt gtgcggaaat caagtcaata
    10141 ttttgtgcag aaagcttggc ttgcagatat tctttgcctt tgggtttttt acctttaact
    10201 tccttgttga tggcttgaat atagaaagcg agacggtcgc gttcttcttg cagggttgga
    10261 atcagcttgc ttttaggcga gctttttttc aactgcgcaa tctgctgttc caattctttg
    10321 gatttttggt tgagttcagc cgctttttgt gtaggaaaat aattgctgaa tgagttgttt
    10381 acggcttcga tattcaactt gcctttggtg gtggcgacaa ccaagttttt accggctgta
    10441 attttagaac ctcttaaatc tgtttctcct gtaaccagac ggatattgcc ttttgcttcc
    10501 aatgaggaaa cttgagcact aggcgcacct gcatttcctc cttttacaga caacagcaat
    10561 tttccgcctg ttttgatgct caggtcggta tgcgcactga tgcggttggc aggttcgatg
    10621 gttaatgtgc cgctacctgc ttcaatattc agccgtccgg ccaatggttt taattcggca
    10681 ttatcttcca aagttttggt cgaaacggta ctccaattga tgttgccgcg cttgactagg
    10741 gcggtaccgg cagtaaggtt gattgcaccc gctctcagcg tggtgttgtc ggcaatttgg
    10801 gaatagcgcg cattgagtgc caatacaccg ttagccacca gcttgttggc agaaggcagt
    10861 ttgtcgtttt gccaaatctg gctgccggta atgcttagat gacggtgtgc gtaggcatct
    10921 acttggttga gcgttacccg ctcgtgttgt gcattaagat gcgtattatg ggtagactcc
    10981 agcttggtat tttctatgct caatgcccgg tcggaatgaa tgttcaatgc cccgcttttg
    11041 gcatggacgt taaggttttt taagtcggca ttaccaccgt tgtttttgat gctgatgtgt
    11101 tttccgttga ttgaattgcg ttgttttccg tcaccaagct gaataccgtt gccggcaacc
    11161 aacgtaatat ctcctgaaga tgaagtgata ttggtattgt ctgctttcag acggccttta
    11221 ccaaccgatc ctgcattgac atcggccttg gctgtcaggg tattgtgacc ggtaaagtcg
    11281 gcattaccgt tggccaataa ggatacatga ccgtctgcag aaacagcatg aaggccgtct
    11341 gaaacgatat tgccctgcaa tgcggtggtt tcgagagcct tggctgcgtt cagcttggta
    11401 ttgcgaagct gaatattgcc tttggctgcc tgaatgtgca gattacccga gttggtacgc
    11461 agattggtat tggtaacatt cagcgagcct gcctccacac ccatgtcttt tgaggcagtg
    11521 agggttttac tggtgccggt aatatgggca gcgttatccg atttcaaatg gatgctggcg
    11581 gcagacaaat ctttatcaac attcaaattc agatctttac ctgtatgaac atacagattg
    11641 ccgggagtat tcagattggt agttttggca gtaatgttat cgtctgccag taaagcaagc
    11701 tgcttgccgc ccttgatact gcctccgttt aagcggatat cggaggtaac tgttgaagct
    11761 tccaaagaaa gcggtttgcc tgcttcgatg tgtgcggtgt ctttggcatc tattacggcg
    11821 gaactgctga tggtgccgtt ggataatacg gtaacatctg ccccggtaat gcgtgtgtta
    11881 ttgcctaatt cggcgttgcc tttgctggaa ctgtatacgg tagtgccagt ctgaatactg
    11941 gcctccttga tgacggtacg gccgccggtc gacagattgg tcgaaccttt agcattgttc
    12001 acatttgctt tgctttcgat aaccaaatta cggccggcat ttaataccgt agcagcaggg
    12061 cggttgccgt tgttttgaac gaccgctccg ttacgcaaac cgatgtcctc cccggtatca
    12121 ataaccaata agcctttgct ctcgatccga ccatcattgg agataaatgt gcctgccgct
    12181 cctttttcgg tggtttcgat ggagagataa gtcggtgaag cttcggtgcc gtcggcagtg
    12241 gtggcgatgc ggccgctgtt ttcaatgcgg cctgacgaag tcacaatcaa ttgcttggcc
    12301 gcttcgagtg tgccggcatt tttgacgcct acgccttttt cattggcaat cagtgtgatg
    12361 ctgtcggcgt acataccgcc cagtgcggca gtatcaaggg caatagtcgg tttcgtaccc
    12421 gctgccgtac ctgcactgat ttcgccgctg gcgtaatcta ctttctgagg accggtagaa
    12481 accgccaggt ttttaccctg taatttcccc tgcaaagcaa ctgcacgagc aagtaccccg
    12541 gtgtagtcgg cttcgccttt atcattccaa cctgctgctc ctacggtcaa tgtgccttga
    12601 cgcacatcaa atcctgtcag tgcaccgtct ttgccgattt ggggcgcacc ggtagttaag
    12661 atgccccgac cgacattttt aaagccgccg ccattaacgg taatgccgtt ggggttggca
    12721 ataatcacgt cggccttttg gccgcctacg gtaacgatgc cgttgagttt gctagccgta
    12781 ccgcgtacct cgttcaaaat caattgcgca ctgcctttga ccacaaacgg attattgtta
    12841 cggtcgttgt ttaacactgc ccctttgttg tcaacatcaa actgcgtata gcggttgtgg
    12901 ctcaatccgc gtccattcgg agtttggata ttcaccaagg gggcaccagt gttggtttta
    12961 aggataacga cctgctggtt tttaggtgct gatttgtcgg tggtaatttg ggcatgggca
    13021 ggcaatacca tactcaggga aaccaaagag cagaccaaag ttttaagggt ggttttgagt
    13081 ttgccgcaaa ggtcgcctga agttttcagt gaaacagaaa ccgaactgcc tgcctgttta
    13141 cctttgccct ggctgttggc agtttcggct actgcaacca tggtgctgtg ctttttacta
    13201 aagataatgc gatgtaaacc tttattcatg tctattccat tttgaagatg aacgtactgc
    13261 gcgccaagta cgtaggtaaa gtttgacggt ctgaggataa ggaaagaccg tctaaatatc
    13321 agtaaaaaat tcagaggtta gaaactgtaa ttcaagttga agccgtaaac ggtgttggtc
    13381 gtctgaaagc ctttgggttt atgaagcggc ttgccggcaa acagatcata agcaaacata
    13441 ccgcctactt tatgccctcc tctgaagccg accactgcac ccatcagctg cttgcccgat
    13501 acatattgtg cactttcgcc agatacgcgg ccatagtccg caccgagata gaactgatgg
    13561 ttcggatgaa aataccaagt taaagtattc tgccagtaga aacctcgctc tccgaaaaga
    13621 ctctgctccc catcaaatcc gcgaacggtg tagcggctgc cgattgacaa tttatcttgg
    13681 gcaaccaacg gcgttttgtt ccattgagct tgaatggcgg ttgcgtagaa aaactgctgt
    13741 ttgcccaaca taaacggggc cgctgcatcc aatccggcgg ttatgatttt catacgggat
    13801 gtgcctggaa tagtaccgcc gccgttttct tcaggtgcgg gcatactttg gcgcataccg
    13861 gtcccgcgtt tgtaagacaa cttgccgtca agctgccaac ggttgaggta agcacggtgg
    13921 cgcaattcgg cttcccagcc tgcagagcgg cggcgttgca cttcgatttc ggcatcgtcg
    13981 atgtatttat aggtttggcg tgtccataat ttcattccga ctgaagtttt atgaagtctg
    14041 ttacgccaaa gcatgcgctc ggcggccaag ctgctctgat attgtttgcc gttgtaatcg
    14101 taattgacgg aatagccttc ggttgcttcg tggtaacgat gtccattgtg attaaaagaa
    14161 aacagccatt tttttacggg caccgaataa tgcacgctgt aacttctgga tccgcttgca
    14221 gtttccgtac cggtggcatc agtcaagtcc gttttgtgcg ccaaaccgcg tccatatgaa
    14281 acataaaaca aatcgcttaa gcccaaaggg ttatcgaacg ataaagcgac atttccttga
    14341 tatttgccgg tcgttttgcc gcccgcatca tctataccga tactgaaccg tatgggttta
    14401 ttctgctgcc atttgatctg taaatcgctt ttgccttctt cttcggacgg tataatctga
    14461 atatctgttt taacactcga caaacgacgc aggttttcca agccctgctc tacatcgcga
    14521 agattgagaa ttttgttcct atataaggga aatttgttat tgaatgcact aatactgcct
    14581 tcggcagact tcccatcccg tttttcttca tagcggatat cccctatttc gcctgctgat
    14641 acccgtaatt tcagaattcc cgaatccata ttctgtggtt ggataatagc ttgggaagtg
    14701 aggtagccac gcacaatcag tatctgttgc gcggcttttt gtagcctgct caaattattg
    14761 gaacctaaac acatcccagt tttaaaagct gtttctttca tgagcacaga aggaagaaaa
    14821 gaaaatttgc gcaccgtctt atcatctaaa ctaatgtaat ttacccgagt acacggtgtt
    14881 tcatcttcac tcaggacata attgttcttc tccaatggtt gctcgaaacg gacatttgca
    14941 tcagttaaca attcagcatc tatgtgctgc tgacgctgca tggaacggat aagttctgca
    15001 tcgttttcat cggcagctaa ggttttaagg ggtatgacag ccaggataac caacaaacat
    15061 ggagcaggaa aaaatttcat gacattaata ttattttagc aatatttact attctgtcat
    15121 aaatttaaaa gtatttacag ttatagaatg agacctttgc aaaattcccc aaaatcccca
    15181 aaattcccac caagacattt aggggatttt ggggaatttt gcaaaggtct cggacagtat
    15241 tttgaacgca gtgcgcgtaa atccgtatgg aaaccatgaa atcccgccac agccgccaga
    15301 catgccaagc cgcattctga tatttctgtt tgcaggataa caggcagctt tttctttaag
    15361 cccaaagaca ggttttgcag atggggcata gatttccttt ttgaaaaata gggattagga
    15421 agttggatgt attttagaaa ggccgcctga aaaggtttca gacgaccttt tgcgactagc
    15481 tgctatttta tttaaagctt ttctctaaca aacgagctaa tatttttcct gtaataaaac
    15541 agataaaaaa cagcatccaa tacgtcagat tggaaaaatc ggtcgtatag agaatcaaca
    15601 tataaagaag cagcatgatg ccgagtgcga tgaattgata atgtttggca aacatcatga
    15661 cctcctcaac tattaaggca aaccgcctga atattctcgt tcaatcgttt cggcaatttc
    15721 cctataacgt cgataccatg accagtcgaa attttcaatg gcatggctcg caaacgtacc
    15781 aaattcaggc atccctatgc ggctacctgc taaagctccg attgtagctc cccaaccagg
    15841 cggattactg aacgtattgt ttggcaatcc taaagattta tcaggatttc ccgtatcgtt
    15901 aagccctgca gattcgcaaa tttcgcaata atatgagctt tgttacgcat tacctgagcc
    15961 tccgacccaa gtcatttcat gaacacatat agtggattaa atttaaatca ggacaaggcg
    16021 acgaagccgc agacagtaca aatggtacgg caaggcgagg caacgctgta ctggtttttg
    16081 ttaatccact ataaaactct cattttgaaa ctccttgtat cgttaatcaa acaatcaaaa
    16141 gggcagatgc cctatccttg cttttacaga cggagtgcct gtaaaagggg atggtttcag
    16201 gcagttttga agtttgtgtt tttatatatt gtcttctggt cgtctgaaaa ggtttcagac
    16261 aacttcttta tctttcagcc tcaagtctta cagtttgccc gacatactat aaatcagctc
    16321 caatacccat tcgtacaatc gccgtttctc gtgcaggatg tctgcttcca acgtcatgcc
    16381 gatttgcagc ggtttttcct caccgtatgc agtgatggtt gatttgtcgg gttttatttt
    16441 cacaagataa acaggttcgc tgctcttcgc caaatcggag gataccatgc ccaatcccga
    16501 caattcctgt ctgcccagtg ccgtttttgc cactgatctt ccgcaagtct aatgcgcctt
    16561 ttctgaccgt ctatctgttg caaaatatgg agtttctggt tttccaaacg ttcgacagtt
    16621 gctttaaggc tgcgcgtttc attctcgtgt atcagcttca gacgacccag ttcctgttct
    16681 gccaacgttt tcttcaaaac tgcctccgtt ttcaactgct gctgcacgct acctcctgcg
    16741 ccgaaacgtg aggtcgaaag cgcaaatagc ttgtcgccag ccttgacctt ttctccatct
    16801 tccacgaatt tcgttgtaat tgtccccgta tccggtgcat acaccctgat tacgcccgat
    16861 gcaggtaaaa tttgtccctc cactgttgtc tttcgcgtat agttaccaaa tatcaaaaac
    16921 aggataatca gtaacgcaga tatcgatgca aatgtcgtcc atagggaaaa tgacaacggt
    16981 cgtgtcagaa tcactttacc cgtcaggctg gtttgccggg caactgtgac ttcagggcgg
    17041 aagaaaggtt gcttgggtct attcataaaa ttgaagttaa gaaagtttca gacgacccct
    17101 agagatcgtc tgaaagatga gaaatatcag cagtaatctg taccgtcagt gtagccgttt
    17161 cctgatttgt ccacagcaaa agcagaaatc ggaccgaccg ttactgtccc tttgggctga
    17221 gtttcttagt aatactcata tgctgcaaca cccataccag ttacagcacc aacagcagca
    17281 ccaactgtgc caccgagaat aatagccggt ccggcaggaa taccaccaga aactataccg
    17341 acacccagac caatacctgc accaattgat cctcctattt gtgcaccatt tgctcctcct
    17401 tcaaggattc tctcccctaa gcctccgcca taaattagct ccagattctc aatggatagt
    17461 tcaatgatgt tatttttcct ttccaaaaaa attagacgta gaatattgag tcaatacacc
    17521 caatatggaa aaaataataa aagcatgaat ataaatcata aaatcaacat catctccttg
    17581 gtagctttca taaataaaaa cgattggtat tgatatacaa attaaaatat acaggtactt
    17641 tgcaagtacc agtaacttat acataaattt tgatttcata gcctagtctt tcattggtta
    17701 gtattcctaa aaattcggct aacaaaaaca tatggcagat atattgaaaa aaaattcaaa
    17761 gtaccctgaa taaaattcaa attccaacta tatttgttaa tgtagtcgag aagaaacata
    17821 tctgataaaa aatatagcac ttgataacaa gctattacta atattacgaa aaatgtaaat
    17881 tgcttccagt ttttcataga atccctcaca aaagttccag aaaatctaac tctatcaact
    17941 gataaatcaa cttcctaact tcttcatgtt ttccctgatt gaagttaacc agtagatttt
    18001 tcaacaataa cggttcattc ttaccgatgt gttctaaaac ttttttcccc aattcatcta
    18061 cgcttatctt catcccattc ccaatcaaat accccttttc caacgtatcc aaattattgg
    18121 catttagtct caacctgacg tcgtctgaaa gcggagtagc gttgggattc gcgaactgtt
    18181 cgagatgaaa agcggtatcg gtgcgttctt tgccgaggaa gtcttcactg aaggcttcgt
    18241 aattgacggg gtcggcaatc atggcagcaa tttgtgcggc agtatcgttg atacgcgtcc
    18301 tatcttgctc ccagtctgag aaactgtggc gcagactttc tatcgtgggg aatttcttca
    18361 ttagccactc gaggtaatta tagccgttgg gcggaaaggt accgacagcg aggtggaagg
    18421 tttcacagcc gagcgggata ggtctgtgcc accaaccgcg cgggatgtag aggacatcac
    18481 ctgcttcaag gataatatcc atatcgatat gttcaggaat ggaaatatca gtatctttag
    18541 tctgttgcat atacaatggc atagggaaat caggggcagt aagttgccaa cgtttcttgc
    18601 cgaaaagctg gatggcatac acatcgcggg tgtcccaatg gtttttataa gattcgtcgc
    18661 tgccaaaagc aagatatcca ctgacaatag tatgtgcgcc agcaaagcgg gcgacttgac
    18721 gggaaatatg atctgaaaac ggctcgttgt taatatggtt atagactaac gacgcaccat
    18781 tcttcatatg ttcgtagata acggatttaa taaaacggta gcgagttttg cccaaagcgt
    18841 cgaaactttc gacgtattct tctttaggaa caattgcacc ttttttacgc agataaaaca
    18901 gcggtgcggt tgggtctgct cgttggtata tctcgttgat atctttccaa gatgcggctt
    18961 cgagattccg agccgctcct ttaaagagct tgggcttttg atacagataa gtctgtcgga
    19021 acgttttagg actaatgccg aagtcgagat ggatgctcat tacttcccct tactcagaaa
    19081 atatttaaaa tttataatgt tacatatatt tacaaatatt aaagtttttt ttgtgtgtgc
    19141 gtcaaggaat tgttgacaat tttagttata gtggattaac aaaaatcagg aaaaggcgac
    19201 gaaaccgcag acagtacaga tagtacggca aggc
//
LOCUS       DAWHRA010000026        19044 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWHRA010000026 DAWHRA010000000
VERSION     DAWHRA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19044
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.026"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            130..1140
                     /gene="waaF"
                     /locus_tag="WIB30_000667"
     CDS             130..1140
                     /gene="waaF"
                     /locus_tag="WIB30_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ3796770.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENSFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            1195..1641
                     /gene="smpB"
                     /locus_tag="WIB30_000668"
     CDS             1195..1641
                     /gene="smpB"
                     /locus_tag="WIB30_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ3796771.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            2007..4042
                     /locus_tag="WIB30_000669"
                     /pseudo
     CDS             2007..4042
                     /locus_tag="WIB30_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(4223..5590)
                     /locus_tag="WIB30_000670"
     CDS             complement(4223..5590)
                     /locus_tag="WIB30_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ3796772.1"
                     /translation="MLDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPLS
                     VKSVQTIMRFCHQHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFANPLDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINSTVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(5700..5966)
                     /locus_tag="WIB30_000671"
     CDS             complement(5700..5966)
                     /locus_tag="WIB30_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796773.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPAAEAPAA
                     EAPAAEAAATEAPAAEAAATEAPAAEAAATEAPAAEAPAAEAAK"
     gene            6439..6921
                     /locus_tag="WIB30_000672"
     CDS             6439..6921
                     /locus_tag="WIB30_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ3796774.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            7008..7820
                     /gene="hpnC"
                     /locus_tag="WIB30_000673"
     CDS             7008..7820
                     /gene="hpnC"
                     /locus_tag="WIB30_000673"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ3796775.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LNGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(7893..8363)
                     /locus_tag="WIB30_000674"
     CDS             complement(7893..8363)
                     /locus_tag="WIB30_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ3796776.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            8454..10295
                     /locus_tag="WIB30_000675"
     CDS             8454..10295
                     /locus_tag="WIB30_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ3796777.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFKIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALSPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            complement(10706..11902)
                     /locus_tag="WIB30_000676"
     CDS             complement(10706..11902)
                     /locus_tag="WIB30_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ3796778.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            12253..12552
                     /locus_tag="WIB30_000677"
     CDS             12253..12552
                     /locus_tag="WIB30_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796779.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEDGAGN
                     HVLSPFAQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNI"
     gene            12552..12737
                     /gene="ccoS"
                     /locus_tag="WIB30_000678"
     CDS             12552..12737
                     /gene="ccoS"
                     /locus_tag="WIB30_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ3796780.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDVR"
     gene            12727..14037
                     /locus_tag="WIB30_000679"
     CDS             12727..14037
                     /locus_tag="WIB30_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3796781.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAIYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKEP"
     gene            14195..14929
                     /gene="dnaQ"
                     /locus_tag="WIB30_000680"
     CDS             14195..14929
                     /gene="dnaQ"
                     /locus_tag="WIB30_000680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ3796782.1"
                     /translation="MIQRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGEAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            14926..15615
                     /locus_tag="WIB30_000681"
     CDS             14926..15615
                     /locus_tag="WIB30_000681"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ3796783.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            15647..16129
                     /locus_tag="WIB30_000682"
     CDS             15647..16129
                     /locus_tag="WIB30_000682"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ3796784.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            16206..16877
                     /gene="rpiA"
                     /locus_tag="WIB30_000683"
     CDS             16206..16877
                     /gene="rpiA"
                     /locus_tag="WIB30_000683"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ3796785.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            16946..17617
                     /locus_tag="WIB30_000684"
     CDS             16946..17617
                     /locus_tag="WIB30_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ3796786.1"
                     /translation="MQIKKIMKWFPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            17796..18542
                     /locus_tag="WIB30_000685"
     CDS             17796..18542
                     /locus_tag="WIB30_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ3796787.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
BASE COUNT         4530 a         4340 c         5404 g         4770 t
ORIGIN      
        1 aattttgaag cccgaacttg aatgttcggg ctttttcata tttgcacgtc cggcgttcgg
       61 aatgcggcaa gataggtttt ccaacggttt cgcggtaaaa ttctgcctgt ctgatttcgg
      121 tttcttgtta tgtccatcaa aatcctgatt atttccccca gttggatagg cgactgcgtg
      181 atgacccagc ccttgttccg ccgtttgaag gaacttcacc ccggttgcac gattgatgtg
      241 ttcgcaccga agtggtcgat ggcggtgttc gagcgtatgc cggaagtgaa tgaaattctt
      301 gagaattcgt tcggacatgg tgcgttggag ttgaaacgcc gttggcgggt cggtagggat
      361 ttggggcggc gcggatacga tcaggttatc gtgttgcccg gttctttgaa atcggcaatc
      421 atcgcgctgg cgacaggtat cggtaaaagg acgggttatg tcggtgaaag ccgttatttt
      481 ctgttgaacg atatacgcag gctggataag gaacgtctgc ctttgatggt ggatcgatat
      541 acggctctcg cgcatccgag tcaggaagat tttgacgggc attcgggatt ccccgagttt
      601 tccattgatg aacggcggcg ggaaatttct gtcgaaacct ttggcttgga tattggaaag
      661 cctgttctgg ctttttgtcc gggtgcggaa ttcgggccgg caaagcgttg gccgacaagg
      721 cattttgccg agttgggcaa acattatctg gcggcgggtt ggcaggtttg gctgttcggt
      781 tcgcaaaaag atgatgaaat tgccgaggaa atcaaccgcc tttcagacgg tatgtgtgtc
      841 aatttgtgcg gcaaaaccga tttgtcgcag gcaatggatt tgctgtcgtt ggcggacacg
      901 gtcgtgtgta acgacagcgg attgatgcat ttggcggcag ctttgggcag gaaggtggtg
      961 gcggtttacg gttcttccag cccgacgcat acgccgcctt tgagcgatag ggcgaaaatc
     1021 gtcagcctgc acttggaatg ttcgccgtgt tttaagcggg aatgtccgct ggggcatacc
     1081 gactgcctca acaggctgta tcccgagaag attgtgcagg cggttgaaga ggcggtatga
     1141 tgggttctgt tttcgggttt atgccgtctg acggcggata ttgataggat gtttatggct
     1201 attgcgaaca ataaaaaagc atttcacgat tttttcattg aagaccggat tgaagccggt
     1261 ttggtcttgg aaggctggga agtcaaagca atccgcgccg cgcgcgtaca gcttaaagag
     1321 agttatatct attggaaaaa agacgcgttt tatctggtcg gctgccatat tacggctttg
     1381 cccacagcct cgacacacat taaaccagat gccgtacgtc cgcgcaagct cttgttgaac
     1441 cagtcggaaa tcaacaaatt aatcggcaaa accgaacgtg ccggctacac catcgtgccg
     1501 ctggatttgc atttttcacg cggaaaaatc aagatggaaa tcggtttggc aaaaggtaag
     1561 aaacagcacg acaaacgcca gagtatgaaa gaggcggatt ggaaacgcga gaaacagcgg
     1621 ttgattaagc atacgcgctg attgtggaaa cagtgcttca gacggtatgg gcaaggctgc
     1681 cgcctgatgt gtaaacgttc tacacctaaa ccagcatccg ctttaatcag cacggcatga
     1741 tgccccaagc tttcgtcaaa ggtttgagca atttgatgat aaattggcat tagccaaggg
     1801 taaagtgcgg tcatttttcc tgaaattttt gtaaataacg agggcaatta tacacatttt
     1861 caaactggct tgacaaaata gcaaaaagca aacgtttact tttaataaac aagaaataaa
     1921 tagagaaaaa tgtgagataa atgacatttt ttgttcagtt ttatgctaaa atccacgccc
     1981 catttatttt tacttgacaa aggaaaattt attatgaaac tcgaagcaag caagcaagca
     2041 agcagaagtt taaaaaatca tttattgtaa gtctattttt ttctattctt tatacctctc
     2101 cgcttttggc tgttgattac gtttacgaca attctaaatt aacaaatgag caaattgatc
     2161 gcttaaaaaa acttagagat aaaagcagcg aatactggaa acaagaaact tatcttctaa
     2221 aaccgacgaa tggtagctct cagaactctc tcaaagttcc cgcactatac tctaaaacac
     2281 accaattcaa aaacatcaac aactcaaaaa agatttcttt ttatgacaag gaatacactg
     2341 aagattacct tgttggcttc gcccgaggtt tcggggttga aaaaagaaat ggggaagaag
     2401 aaaaaacact ccagcaatat tttaaggact gtgtaaatac tgggaattcc aataatgata
     2461 agtgtgaaca accatccttc ggaaactctg gcccaagatt aataaagagc gatattttcg
     2521 ccttagctcc acaaataaaa aatagccata tcaacagcga aattttatct gtaggtaatt
     2581 atatcgaatg gcttcgccca acacttaata agcttgctgg ttgggaagag catctatatt
     2641 ctggtttaga tccattccac tatattgagg ttacagacaa ctcgcacgtt atcgggcaaa
     2701 cgattagttt ggatgaattc cacttagaaa attctctctg ggaaccccgt tgggattctg
     2761 atgttgggga gttaaaaaca acgaatgcag acatccgttt taatactaaa agtgaaagtt
     2821 tactcgtaaa agaagattat gctggcggag ctcgttttcg ttttgcttac gatccgaaag
     2881 aagcgacaaa tgccacatta atttttgaga aaaatataac tggcacttcc gacatcatct
     2941 tcgaaaatcc aatagacgat ttaaaaagtc tcgacgggca tcaaattatc aaagtaaatg
     3001 gcacagcaga taaacacgca ttccgtcttt ctggcaaaca ccaaaaggga atttatacgc
     3061 tttctttaca acaacgccca gaaggctttt ttaccaaagt gcaagaacgc gatgatattt
     3121 cgatttatgc acaacaggct caagctgcca ataccttatt cgccttgcgt ttgaacgaca
     3181 aaaacagcga tattttcgac cgcactttac cacgcaaagg cttgtggttg cgtgtgattg
     3241 acggacattc caaccaatgg gtgcaaggca aaacagcacc tgtagaaagc aatcgtaaag
     3301 gtgtgcaact cggcggcgaa gtattcacat ggcaaaacga aagtaatcaa ctttctattg
     3361 gcttaatggg cggacaggca gaacaacgca gtactttccg caatccagat acagacaatc
     3421 ttacaacggg aaatgtgaaa ggccttggtg caggcgttta cgccacttgg caccaacttc
     3481 aggacaaaca gacaggtgcg tatgcggata gctggatgca atatcaacgc ttccgccacc
     3541 gtatcaacac tgaagatggt acagaacgtt ttacttcaaa aggtattact gcctcaattg
     3601 aagcaggtta caatgcgtta ttggcggaac acttcactaa aaaggcaaca gccttcgtgt
     3661 ttacctacaa ccacaggcac aattgactta tttgggggta aacggaaaat tcagcgatag
     3721 cgaaaatgcc cacgtgaatt tacttggctc ccgccaatta caaactcggg ttggcgttca
     3781 agctaaagct caattttcac tctataaaaa tatcgctatt gaacctttcg ccgctgtgaa
     3841 cgcgctttac cacaacaaac ctttcggtgt ggaaatggac ggagagcgtc gagtgataaa
     3901 caataagacg gcaattgaga gccaattagg tgtagcagtg aaaatcaaat ctcacttaac
     3961 cttacaagcc acattcaacc gccaaacagg caaacatcat caagctaaac aaggtgcgtt
     4021 gaatttacag tggacgtttt aagttaagtt gtaacaaaat aaaatcggca gggaaattca
     4081 ctgccgattt ttttatttta aaagtgcggt catttttcgt gtaatttcca ccaattttta
     4141 accgcactca cgagctcgcg ctaaaccgac aatcggatct attgtccctt tctttgttgt
     4201 gtatgcaaat cagaaatgcc ggttacggaa gcagtttgcc cggattcata atgttatatg
     4261 gatcaaggtg ttgtttgatg cttttcatca gggcgatttc ggcaggcgtg cgtactttgt
     4321 ccagccactg ttttttgatg atacctatgc cgtgttcggc ggcaatcgtg ccgttgcagg
     4381 caaggacgtt gcgatagact gtgctgttga tgtcgttttc gtaacgatag acttcattgc
     4441 tgaggatttc gggcaggaaa gtattgtagt gcaggctgcc gtcgcccaga tgtccgaagc
     4501 agacgatttg tatgcctttg aaattctgtt ccaaatcttt ggcgcaccgg cggacaaagt
     4561 cggcaacgcg cccgatagga acggcaatat cgtgtttgat gctggtgccc agtttgcgtt
     4621 gcgatgcgga gatgttttcg cgcaacgccc acatatggat acgttcttgt tcgctttgcg
     4681 ccaacacgct gtcggtaaag ccttttttat aaagaaattc gacaagccga tcatcaagat
     4741 tgctgtcggg taatgagtcg gtcaactcaa gtaaaatatg ccattctgaa tgtgtcggca
     4801 gggggagttt gctgaattcg gaagacaatt cggcggcaaa acggccgatc agctcaaaac
     4861 tgcataggcg ttcggcaaag tgtgcttggg tttcggtcag caggcggacg gcggattcga
     4921 tgtcgggtat gccgacccat gcggttgctt tgtctaaggg gttggcaaac agcttgagcg
     4981 tggcggcagt gataatgccc aatgtacctt cgctaccgat aaacagatgg cgcaggtcgt
     5041 agccggtggt gtttttatgc aggggatgga gatgggaaac cagttcgccg ttggggagga
     5101 cgacttccaa accgataacc aggtcgcgca tcgtgccgta acgcaatacg ttcaaacctc
     5161 cggcattgca ggcgatgttg ccgccgattt ggcacgagcc ttcgctggcg agactgagtg
     5221 ggaacagcct gtttgaggct tcggctgcct gttggacggt ttggagtacg gaacctgctt
     5281 cgacggttat gcagttgtct gacaaattga tgctgcggat gcggttgagt ttggaaaggt
     5341 tcagcaatac gccgttttcc gatactgccg cgccgcacaa accagtattg ccgccttgcg
     5401 gcgtaaccgg aatacggtgt tggtggcaga aacgcataat ggtttgcacg cttttaacgc
     5461 tgagcggctg caaaatgatg tctggtgcag acgtaaagcg gttgcgctgg tcttttaaaa
     5521 gcgtcggaga aggttcggca atttcatctg ccggtaggag tcgggaaaat tcggtatgaa
     5581 ggtcaagcat gtttgtcgga agggtaggga gaagattgag gaagggaatc tgatgccgtc
     5641 tgaaaaatca aaaaagcagg atactgaacg tatcctgctt ttttgcaagc ggaaaatgct
     5701 tattttgcag cttcggcagc aggagcttca gcggcaggtg cttcggtagc ggcagcttca
     5761 gcggcaggtg cttcggtagc ggcagcttca gcggcaggtg cttcggtagc ggcagcttca
     5821 gcagcaggag cttcggcggc aggtgcttca gcagcgggag cttcggcggc aggtgcttca
     5881 gcagcgggag cttcagcggc tttttcaccg ccgcaggctg ccagaaccaa agacaacaaa
     5941 gcagcggcaa acagggattt tttcatttta gtaaccttta gaaccaattt aaattattga
     6001 aacaaatagg tctttatcga ccatactaca aaccgggttg gaaattcatc gctgaatttg
     6061 cgtaattatg cactatggaa taagggattg caatgtcgta cagatgggaa aaaggtttga
     6121 atgaaaagtt ttctggcggg cggattgata aatcctgtaa aacggttttg ctgttaaaaa
     6181 ttcgataggg ataaatatta tagagattaa tataatttca gtatcttgta tgatttatta
     6241 aggcggattg ggaaagtcgg cagacaggtc ggaatggttt taagaatgtt gtaaaaaagt
     6301 tacaccgcaa taaaataaca aagatttaca ttgaaatatg tgctgtacag tttatgaaca
     6361 ggttaacggt aatcggccgg cgcggcggtt gtgttagaat gcgcctgtaa ttaatctgct
     6421 gttcctgaaa ggacaaatat ggctatcgtg aaaaattccg tggtttcgct gcattatgag
     6481 atgtatgatg ccaacaatca gcttttggac aaaaccgaag aaccgattgc gtatctgcac
     6541 ggcggttacg acggcatttt ccctttggtg gaagaggcgt tgcacggtaa ggatgccggc
     6601 gatacggtcg atgtggcgct gtcgcccgac gatgcgttcg gcgagcagga tccggagttg
     6661 gtccgtatcg aagatgcggg cgtgttccct gttgaagtcg aagtcggcat gatgtttgaa
     6721 gccgacgatc ctgaaaccgg cgatgttgtc gtctatcgtg taaccgatgt tgccgacggc
     6781 aaggcggtgg tggacggcaa ccatcctttg gcaggcatga aaatccgctt taaggctaca
     6841 gttgaaagcg tgcgcgatgc atccgatgag gaaatcgcac acggtcatgt ccacggcccg
     6901 cacggtcatc accaccactg attgccggca gcttggaggt atctgatgcc gtctgaaaag
     6961 ggtttgttcc gtttcagacg gcattttgtt tgatggatgg agaaggtatg tcggttgggc
     7021 attatgagaa ttttcccgtc ggttcgctga ttttgccgcg caggttgagg aagccggttc
     7081 acgcggtgta cgcgtttgca cggacggcgg acgatatggc ggacgagggc agtatgccgt
     7141 ctgaagccag gttggcgggg ttggagggtt tgcagcgcga gttggacgtg ttggcatcgg
     7201 gcggccggtc ggcgcatcct ttgattgcac gattggatgc cgaggcggtt gtgccgttcg
     7261 gtttggattt gcagccgttt tatgatttgc tttctgcatt ttctcaggat gtggttaaaa
     7321 caaggtatgg aaatttcggc gagctggccg attattgtcg gcgttccgcc aaccctgtcg
     7381 gacgcattat gctggctttg tacgggaaaa cggatgcggt gtgcgtggcg caaagcgacg
     7441 gcatctgtac ggctttgcaa ctggtgaatt tttggcagga tgtggctgtg gattggcaaa
     7501 aaggcagggt ttatattccg caggacgatt tgttgaaatt cggtgtttct gaggaacaga
     7561 tcgcggcagg acgggcggat gcggcgtttc agcggctgat ggcgtatgag tgtcggcgtg
     7621 cgttccgtat gctgaaggcg ggttcgcctt tggcaaggga actgaatggg cgtatcggtt
     7681 tggaactccg tatgattgtg ttgggggcgc agttgatttt gcagaaactg gacgcgtgcc
     7741 gatacgatgt gtttgcacaa cgccccgttt tggataagaa ggattggttg attatgctga
     7801 aaaaggcgtt gtggaaatga ttgggggaaa atgaatgccg tctgaaaggg gttgttccgc
     7861 ttcagacggc attttgcagg cggttggtat ggtcaggcga tttcatgcgg gttcaatctg
     7921 gcttgatctt ctgcacccaa gccgatgagt ttgcgcagtc gggtcatgta gaaattgaca
     7981 tccaaaccgg tgccgtagcg ttgcgccgtc cacagtgttt ccgccagtgc ctccatcatt
     8041 tcgtgttcgg cttccagcca gccgcgtttg gcgcacaggg tgtcgtggat tgcgcgtatg
     8101 ccgtgcggct ggtctatgcc cgcctgttct tggacggaca gatgcagcga catatgcagg
     8161 aaggggttgc tttcgccgtt ttcgggcagc cagtcggtgt ccagatggtc ttcgatgcgt
     8221 tcgagatagc ggtggtattc gggatgggct tcgacaatgc ggagggcttt ctgttccagt
     8281 gcgctcagtt gcagcggatt gagccgctgc tgccacacgc gggcgaaaaa gcggcggaca
     8341 tcgtgggtat tgacatcgta catggcgggt tcgataagat ggttatggaa ataggttcgg
     8401 ataatggcgg gttttacata aattaaatcc caccgtcaac cttaccattt ataatgcaga
     8461 caaattattt gtctgaaacg gattcgatta tgaaaaaact gatttgcctg ttcgccgtat
     8521 ttttgatgtt gtgcggacga gctttcgcgc tggatgcgaa cgatctgctg ccgccggaaa
     8581 aggcattcgt gccggagctt gccgttgccg acgacggtgt gaacgtccgt ttcaagattg
     8641 ccgacggata ctatatgtat caggcgaaaa tcgtcggcaa gaccgatccg gcggatttgt
     8701 tgggacagcc ttctttcagc aagggcgaag agaaggaaga cgagtttttc ggcaggcaga
     8761 cggtttacca tcacgaagcg caggttgcct ttccttatgc aaaggctgtc ggcgaaccgt
     8821 ataaattggt tttgacctat cagggctgtg ccgaagccgg cgtgtgctat ccgcccgtgg
     8881 ataccgagtt tgatatttcc ggcaacggca cttaccatcc gcaaaccgac gaaccggcat
     8941 ccgccaaaga ccgctttttg cagccttcct ctcaaaacgg cagcggggcg ttgtcgcccc
     9001 cgaaggggga tgagggcggc gacagccgtt tcaagctgtc ttgggatacg ctcaacgcca
     9061 atcttttggc gttttttctc gccggtttgg gtctgagttt taccgcctgt atgtatcccc
     9121 tgttgccgat tgtttccagt attgtggtcg gcgacaaaaa ggcgggcaag gcgcgggcgt
     9181 ttgtgctgtc cgtcgtttat gttcagggtt tggctctgac ttatacgctg gtcggcattg
     9241 ttgccggact gacgggcgcg ctgctgaccg tatggttgca gcaggcttgg gtggtgttgg
     9301 cggcatcggc tttaatggtc gtcttggcac tgtctatgtt cgggctgttc aacatccagc
     9361 ttcccaacgc cgtgcagtcg tatttccaaa accagagcag caggctttca ggcggtaaaa
     9421 tcgtttccgt ctttattatg ggcatattgt ccgcgctgat tgtcgggccg tgcgtcgccc
     9481 cgccgctggc atttgctttg ggctacatcg gtcagacggg cgatgcggtt ttaggcggtt
     9541 tggcacttta cactttggcg ttgggcaccg gcgttccgct gattgccatc ggcacgttcg
     9601 gcgggcatat cctgcctagg gcaggcgatt ggatgaatgc cgtcaaatac gctttcggct
     9661 ttatcctgct tgccgtcgcc gtttacctcg ccacgccgca cttgccctat tatctcgtcg
     9721 tcgcgctgta cacgctgctg atgctggttc ctgcctgtat gctgctggtc aacggacgca
     9781 ggcagaaacg ccgtccgaaa gctgtggcat tcgcattggg cggcatattg ctgataggcg
     9841 gcgcgtggtt cggctggcag ggcgcaaacg gcaaaacgac cgcgctgcac catttcctga
     9901 ccctcaatcc accagccgaa gcaggcaaat cttcggaaca cggcaaaatg tttgccgata
     9961 ctgccgcgct gaaggcagcg atggatacgg cgttgaaaga acatcccgac aaacccgtcg
    10021 ttttggattt ttatgccgac tggtgtattt cctgcaaaga aatggcggca tacacgctca
    10081 atcagccgga agtgcatcag gcggttgata tggaacgctt tttccaaatc gacgtaaccg
    10141 ccaacacgcc tgaacatcag gcgttgttga aagaatacgg tctgttcgga ccgccgggcg
    10201 tatttgtcgt ccgctccgac ggcagccgca gcgagccgct gctgggtttt gtcaaagcag
    10261 acaagtttat cgagtggtat gaacaaaacc gctgattccg atgtttcaaa tgccgtctga
    10321 agttctcaga cggcattttt gtcagaaagg aaaacggcaa tatccactat acacacggaa
    10381 gcagtctaaa gatgtgaaaa ccgtgtgtat aagttagttt gcgtattgaa attaaaagat
    10441 aaaaatacga tgattaaaaa ataggcataa gaaaagataa acacagccgg tgtttgccgg
    10501 tgtgtgtatc ttttgtggaa aaacatctta atgtcatgta ttttatggaa aataataaca
    10561 tgattaaaaa atagtcaatc attaaggttc aggtaagcgg ctgcaaccgt caatgtctga
    10621 aaagaggcag tgctgtttaa gcatggaaag ccggataaaa aaaactgcct ccgtttccgg
    10681 aggcagttcg tgtgcggata aaaccttaca gaccgctcaa acgggcagtg tcgtgggcaa
    10741 tcatcagctc ttcgttggtc ggaatgacca cggcaacggc tttgctgtcg gcggtggtaa
    10801 tcacgccggc gttgccgaag cgggctttca ggttggcttc ttggtcgatg ttcagaccga
    10861 ggaagcccaa gtagccgatc acgcgttcgc ggatgatgtc ggagttttcg ccgatgccgc
    10921 cggtaaagac cagtgcgtcc aaaccgcctg cggcaaccgc catactgccg atgtatttgg
    10981 caaggcggta gataaacata tccaaggcca atttcgcgcc tttatgcccc ttggcggctt
    11041 cttcttcaat ggtacggcag tcgttggaca ggccggaaat gccgagcaga ccggattttt
    11101 tattcagcat ttcagtgatt tgggcgatgg tcatattggc gttttcggcg aggaagccga
    11161 atacggaagg atcgatgtcg ccgctgcgcg tacccattac cagcccttcc agcggggtca
    11221 ggcccatact ggtgtcgcgc gattcgccgt tggcgacggc ggtgatggac gcgccgttgc
    11281 ccaagtgggc aatgaccata cgcaggtctt ttttgtcttt gccgaggaag cgcgcggttt
    11341 cgtcggcgac gaagcggtag ctggtaccgt gcgcgccgta acggcgcagg ccgtattttt
    11401 catacaactc ctgcggaacg gcgtatttgt aggcgacttc gggcatggtt tggtggaagg
    11461 aggtatcgaa tacgacgacg ttgggcaggc ctttgaaaat gctttgcgcg gcacgcaggc
    11521 ccaagaggtg ggcggggttg tgcagggggg cgagcgggat gcatttttcg atgccggcaa
    11581 tgacttcgtc gtcaacgagg atggattcgc tgtacagttc gccgccgctg acgacgcggt
    11641 ggccgatggc gccgatgcgg ctgtcgaggc cgtgggcttt gagttcttcc atcagggctt
    11701 cgaccgcgcc ggtgtggtcg ggatgtgcgg acagatcgac tttgtgtttt tcgccgttta
    11761 ctttgaatgt gatgtaggca tcgggcaggt tgagtttttc ggcaaggcag ctcagcagga
    11821 cttcgccgct gccgttatcc aggaccgcgc ctttgaggga cgagctgccg cagttcaaaa
    11881 ccaagatcaa tttttgggac attttcttac tccggaaagt ttcagacggc attggaatcg
    11941 gacacggata ctaaccggat tcgtgccgaa tccgttttgc cttcctgggc gggaaagtag
    12001 tggggccgtc tgaaaaggtt gataaaagaa gcaggctatt ctagcaaaaa atctttgcaa
    12061 ttgcttggct taatcgggcg tttgcgtgaa aatggcggaa gtcgggcatt ttgacggaaa
    12121 aaagggggat atgcggtttg ccggtgtttt cggctgggtg agggagaatt tctttgcatt
    12181 cttcccgtcc aaactggtac attcgccctg tttttagttg gtgtcgtctg aaaaaaggcg
    12241 ggtgggaaat ttatgcatta tttcagtatt catgaccaat cgggacggca tataggcttt
    12301 ttcatcctgc ttgccgacga tgagtcggag gcgcggccgc agagcggacg ttttgccgtt
    12361 aaattggaag atggagcggg caaccatgtc ctgtcgccgt ttgcccagac ggaaatcccc
    12421 caatattggc gcgtggtcaa agaccgtatc gagctgtttt ttgacgaagc ccccgtcgga
    12481 actttgcgta acgaatatct gaccgtatcg gggcagactt ttgttttgaa cgatttgaca
    12541 gggaatatat gatggaaagt atgtttatat tggtgcccat cagcattatt ttggcgtttg
    12601 tcatcggctg gtttttttgg tggtcgggca aaaacgggca gtttgacgac ttggaagggc
    12661 cggcgcaccg catcctgatg gatgatgatt cgacaagtaa actgattgaa aaagagaagg
    12721 aaaacgatgt acgctaaaaa aggcggtttg ggactggtta aaagccgccg tttcgcacct
    12781 cttttcgcta cgcagtttct cggcgcgttc aacgacaatg tgttcaaaac cgcgctgttt
    12841 gtgatgattg ggttttacgg tttggggcaa aacggcttcc tgcctgccgg acagatgttg
    12901 aacttgggcg cgttgttgtt tattttgccg tatttcctgt tttcctcgct gtcggggcag
    12961 ttgggtaaca aattcgacaa ggccgttttg gcgcgttggg tcaaggtgct ggaaatgatc
    13021 attatggcgg tggcggcata cgggttttat atccggtctg ccccgctgct tttggcgtgt
    13081 ctgttttgca tgggcgcgca atcgacgctg ttcgggccgc tgaaatacgc catcctgccc
    13141 gattatctcg acgacaaaga gttgatgatg ggcaacagcc tgattgaatc gggtacgttt
    13201 gtcgccatcc tgttcggtca gattttggga acggcggtgg caggtgtacc gccttatatt
    13261 gtcgggatac tggttttgct ggtcgccgta ggaggcacgg tcggcagcct gtttatgccg
    13321 tccgtacccg ccaaggctgc cgatacacaa attgagtgga atattgtccg gggtacaaaa
    13381 tccctgctgc gtgaaacggt gcggcacaag cccgttttta ccgccattat cggtatttcg
    13441 tggttttggt ttgtcggcgc gatttatacc acgcaactgc cgacctttac ccaaatccat
    13501 ctaggcggca acgacaatgt tttcaacctg atgcttgccc tgttttccat cggtattgcc
    13561 gccggttcgg tactgtgtgc caagttcagc agggaacggc tgatgttggc ttgggtaacg
    13621 gttggtgcgt tgggtttgac ggtttgcggt ctggttttgg tgtggctgac gcacggacac
    13681 cgttttgaag ggctgaacgg cattttttgg tttttatcgc aaggatgggc ataccccgtg
    13741 atggcggtga tgacgctgat cggctttttc ggcggatttt tctccgttcc gctctatacc
    13801 tggctgcaaa ccgccagcag cgagactttc cgcgcccgcg ccgttgccgc caacaatatc
    13861 gttaacggta tttttatggt ttccgccgcc gttttgagcg cggtgttgct gtttttgttt
    13921 gacagcattt ccttgttgta tctgattgtc gccttgggca atattccgtt gtcggtattt
    13981 ttgattaagc gcgaaaggcg gtttttaggc gcggcggcaa tcaggaaaga accttgaatg
    14041 ccgtctgaaa agtttgtccc cgcaatacac aatacgcctg cccaatgttg tgaacacact
    14101 gtgaataagt gggtttgtgc attgaaatga aaacagaaaa atacgctgcc taaaaaatag
    14161 tcagcagatg gaaaatttga agaaagaagc agacatgatt caacgccaaa tcatcctcga
    14221 taccgaaacc accggcctgt atgccgatgg cggcgaccgc ttggttgagt ttgccggtct
    14281 ggaaatggtc aaccgccaaa tgaccgacaa aaacctgcac ctttatgtcc accccgagcg
    14341 cgatatgcct gaagaggcgg cgagggtgca cggtctgacg atagaggttt tggaagggaa
    14401 aaacgcaccg ccttttgcag aggtcggcag gcagattgcc gattttctgc gcggtgcgga
    14461 gttgattatc cacaatgcca aattcgatgt gggcttcctc aatatggagt tccgccggat
    14521 ggggctgccg actgtcgagg aactcggctg taccgttacc gataccctgg cgatggcgcg
    14581 cgaaatgttc cccgggcaga aagccagcct agatgccctg tgcaaccgtt tttccgtcga
    14641 ccgcagcaag cgcgtcctgc acggcgcatt gatcgactgc gaacttttgg gcgaagtcta
    14701 tcttgctatg acgcggcggc agttcgacct gatgggggag gccgcagagg aaaaaatgga
    14761 aaccaaaccc gtggtgcata ccgaaaccaa acgcagcggt aaactgaaag tcatcagggc
    14821 ggacgaaaac gagttggcgg cgcacgggca atatttggac ggcttgggcg aagcctgtat
    14881 ctggcgcaag gaagccgtgc cgtctgaaaa cggcgggacg gacgcatgaa gcgcaaaaat
    14941 atcgcgctga ttcccgccgc cggcatcggg gcgcgtttcg gtgcggacaa acccaagcaa
    15001 tatgtcgaaa tcggaagcaa aaccgtttta gaacatacga ttgggatttt tgaacggcat
    15061 gagggcgtcg atttgaccgt cgttgtcgtc tcgcccgaag acacgtttgc cgataaggtt
    15121 cagacggcat ttccacaggt tcgggtgtgg aaaaacggcg gacagacccg cgccgaaacc
    15181 gtccgaaacg gtgtggcaaa actgttggaa atcggcttgg catcggcgga tgacaatatt
    15241 ctggtacacg atgccgcgcg ctgctgcctg ccgtctgaag ctttgacgcg gttgatagaa
    15301 caggcgggca acgctgccga aggtgggatt ttggcaattc ccgttgccga tacgctcaag
    15361 cgcgcggacg gtgggaacat tagtgcaacc gtcgagcgga cgagcctttg gcaggcgcaa
    15421 acgccgcagc ttttccgcgc cgggctgctg caccgcgcat tggctgcgga aaacttggac
    15481 ggcattaccg atgaagcgtc cgccgtggaa aaattgggtg tgcgtccgct actgatacag
    15541 ggcgacgtgc gcaatttgaa actgacgcag ccgcaggacg catacatcgt caggctgctg
    15601 ctcgatgccg tctgaacttc aattcaaaac atcaggaaca caaacgatga cgaacatccg
    15661 tatcggacag ggctacgatg tccaccaact gaccgaaggc agaaagctga tacttggcgg
    15721 agttgaaatc ccatttgaaa aagggctgct cggacactcc gatgccgatg cgctgctgca
    15781 cgccgttacc gacgcgctgc tcggtgcggc aggtttgggc gatatcggca gccatttccc
    15841 cgacaccgcc gcagagttca aagatgcgga cagccgcgtc cttttgcgtg cggcgtatca
    15901 aagcgttcag gcgcagggtt ggcaggcggt caatgtcgat acgaccgtga ttgcacagaa
    15961 acccaaactc gcgccgcata ttccgcaaat gcgtgccaac atcgccgccg atttgggcat
    16021 agacatttcg tgcgtcaaca tcaaaggcaa aaccaacgaa aaactcggtt atctcgggcg
    16081 catggaaggc atagaggcgc aggcggcggt attgctggta cggatttgaa cgcggcgggc
    16141 aggacgtgca aaaaaatgtt aagatgacgg ctgtttgttg atacggtttc ccaaaggaca
    16201 aaaatatgac gacacaagac gaactcaagc gcatcgccgc cgaaaaagca gtcgaattcg
    16261 tacccgaaaa cgaatacatc ggcatcggta ccggttcgac catcaacttt ttcatcgaag
    16321 ccttgggtaa gagcggcaaa aaaatcaaag gcgcggtatc tacttccaaa aaatccagcg
    16381 aactgcttgc ccaatatgat attcccgtcg tttccctgaa cgaagtatcg gggttggcgg
    16441 tctatattga cggtgcggat gaagtgaacc acgccctgca aatgattaaa ggcggcggcg
    16501 gcgcacacct caacgaaaaa atcgtcgcca gcgcatcaga aaaattcgtc tgcattgcgg
    16561 atgaaagcaa atatgtttca cgtttgggca aattccctct gcctgtggaa gtcgtcgaaa
    16621 gcgcgcgttc ccttgtttcg cgcaaactgc tcgctatggg cggacagcct gaattgcgta
    16681 tcggctacac cactttttac ggcaaccaaa tcgtcgatgt ccacggcttg aatatcgaca
    16741 aaccgctgac gatggaagac gaaatcaaca aaatcaccgg cgtactcgaa aacggaatat
    16801 tcgcccgcga tgccgccgat gtgttgattt tggggacgga agagggtgcg aaagtcatct
    16861 atccctgcca agggtaatcc tgacgttaag atgccgtctg aaatccgatt ccgatatgtt
    16921 cagacggcat cggcatttga aaaatatgca gattaaaaaa atcatgaaat ggtttcccgt
    16981 cgccctgtcg cttttgggtg cgttgggtta tacggggtac ggcagcgagg cggtgcggac
    17041 ggcggttgcc gtactcgacg tactcggcgc ggcaggggac gcgggttccg acgcgcccgc
    17101 ccgccgacgg gcatcggcga aatccggcca ccgctacaca ggcacggtgt ccaaagtcta
    17161 tgacggcgac acccttcacg ttatcgacgg cgacggcgcg aaacacaaaa tccggatggc
    17221 gtatatcgac gcgccggaga tgaaacaggc ttacggcacg cgttcgcgcg acaacctgcg
    17281 cgcggcggcg gaaggcagga aagtcagcgt gcgcgtgttc gataccgacc gctaccagcg
    17341 cgaagtggcg caggtttctg tcggcaaaac cgatttgaac ctgatgcagg tgcaggacgg
    17401 ggcggcgtgg cattataaaa gttatgctaa agaacagcag gataaggcgg attttgccga
    17461 ctatgccgac gctcaaattc aggcggaaag ggaacgcaaa ggattgtgga aagctaaaaa
    17521 tccgcaagcg ccgtgggcgt accgccgagc aggcaggagc ggcgggggca ataaggattg
    17581 gatggatgcc gtgggcgaat ggttgggcat ttggtaaaga cagcgcacgg ctttgaacgg
    17641 caaatgccgt ctgaaaaccg cgcgccctgc atttttgcgg agacgcgcaa ccgccgtctg
    17701 ccgcgataaa aaagttcagg cggcggtttt tacggtaaaa tggcggatta ttttgcaacg
    17761 gcggcagttc cgccggtttt tgatacggac ggtatatgga ttttcgtttt gacattattt
    17821 acgaataccg ctggatgttc ctttacggcg cactgacgac cttggggctg acggtcgtgg
    17881 cgacggcggg cggttcggta ttgggtctgt tgttggcgtt ggcgcgcctg attcacttgg
    17941 aaaaagccgg tgcgccgatg cgcgtgctgg cgtgggcgtt gcgtaaagtt tcgctgctgt
    18001 atgttacgct gttccggggt acgccgctgt ttgtgcagat tgtgatttgg gcgtatgtgt
    18061 ggtttccgtt tttcgtccat ccttcagacg gcattttggt cagcggcgag gcggcaatcg
    18121 cgctgcgtcg cggatacggg ccgctgattg ccggttcttt ggcactgatc gccaactcgg
    18181 gggcgtatat ctgtgagatt ttccgcgcgg gcatccagtc tatagacaaa ggacagatgg
    18241 aggcggcgcg ttctttgggg ctgacctatc cgcaggcgat gcgctatgtg attctgccgc
    18301 aggcattgcg ccgcatgctg ccgcctttgg cgagcgagtt catcacgctc ttgaaagaca
    18361 gctcgctgct gtcggtcatt gctgtggcgg agttggcgta tgttcagaat acgattacgg
    18421 gccggtattc ggtttatgaa gaaccgcttt acaccgtcgc cctgatttat ctgttgatga
    18481 cgactttctt aggctggata ttcctgcgtt tggaaaaacg ttacaatccg caacaccgct
    18541 gattggagtc atatcaacaa aaagctgccc gggtattcat ttgaggcagc ttttttgtgg
    18601 caaatttatc gtggattaac aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga
    18661 acgattctct aaggtgctga agcaccgagt gaatcggttc cgtactatct gtactgtctg
    18721 cggctcgccg ccttgtcctg atttttgtta atccactata aattcaaaga gaaaaagcta
    18781 tgccgtctga aagtctttca gacggcatct gtcagcctca cgcaagaggg aatcccatcc
    18841 atacggaaac ctgcaccacg tcattcctac gaacctacat cccgtcattc ccacgaaagt
    18901 gggaatccag aacgtaaaat ctgaagaaac cgttttatcc gataagtttc cgcaccgaca
    18961 gacctagatt cccgcctgcg cgggaatgac ggcggagcgg tttcggtttt tccgataaat
    19021 tcccgtggct ttgggtttcc ggat
//
LOCUS       DAWHRA010000027        18876 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWHRA010000027 DAWHRA010000000
VERSION     DAWHRA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18876
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.027"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..414
                     /gene="pgk"
                     /locus_tag="WIB30_000686"
     CDS             <1..414
                     /gene="pgk"
                     /locus_tag="WIB30_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ3796788.1"
                     /translation="SVPLPTDVVVAKAFTADEEAVVKDIADVAEDDMILDIGPKSAAE
                     LADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEAIAQSKAFSIAGGGDTLAAIAKFG
                     VTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA"
     gene            complement(481..729)
                     /locus_tag="WIB30_000687"
     CDS             complement(481..729)
                     /locus_tag="WIB30_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ3796789.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(820..1737)
                     /gene="ftsX"
                     /locus_tag="WIB30_000688"
     CDS             complement(820..1737)
                     /gene="ftsX"
                     /locus_tag="WIB30_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ3796790.1"
                     /translation="MSIIHYASLHIESARTALKELLRQPFGTLLTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRIANVRFIGKEDG
                     LAELQSNLDQNLVSMLDGNPLPDAFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAISLGLCGWLLSTVRPLVDAIFKPYGLNIGWRFFYPEEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(1734..2384)
                     /gene="ftsE"
                     /locus_tag="WIB30_000689"
     CDS             complement(1734..2384)
                     /gene="ftsE"
                     /locus_tag="WIB30_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ3796791.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(2578..3060)
                     /locus_tag="WIB30_000690"
     CDS             complement(2578..3060)
                     /locus_tag="WIB30_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ3796792.1"
                     /translation="MKYLNLAAIALAATFAAHTASADELAGWKDGTAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            3184..3537
                     /gene="arsC"
                     /locus_tag="WIB30_000691"
     CDS             3184..3537
                     /gene="arsC"
                     /locus_tag="WIB30_000691"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ3796793.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIAEHPALLERPIVLANG
                     KAAVGRPLENIEAIL"
     gene            3534..4190
                     /locus_tag="WIB30_000692"
     CDS             3534..4190
                     /locus_tag="WIB30_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ3796794.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIDSVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            4306..5700
                     /locus_tag="WIB30_000693"
     CDS             4306..5700
                     /locus_tag="WIB30_000693"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ3796795.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITANGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            5721..6116
                     /locus_tag="WIB30_000694"
     CDS             5721..6116
                     /locus_tag="WIB30_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796796.1"
                     /translation="MPSDVGIRLQTAFKWKLKMKKIFYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNKLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            6120..6611
                     /locus_tag="WIB30_000695"
     CDS             6120..6611
                     /locus_tag="WIB30_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3796797.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDFGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDI
                     DKV"
     gene            complement(6735..9329)
                     /gene="mutS"
                     /locus_tag="WIB30_000696"
     CDS             complement(6735..9329)
                     /gene="mutS"
                     /locus_tag="WIB30_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ3796798.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPETHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELEDQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            9773..10777
                     /gene="gap"
                     /locus_tag="WIB30_000697"
     CDS             9773..10777
                     /gene="gap"
                     /locus_tag="WIB30_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ3796799.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSILERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(10847..10966)
                     /locus_tag="WIB30_000698"
                     /pseudo
     CDS             complement(10847..10966)
                     /locus_tag="WIB30_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11035..11715)
                     /gene="tsaA"
                     /locus_tag="WIB30_000699"
     CDS             complement(11035..11715)
                     /gene="tsaA"
                     /locus_tag="WIB30_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ3796800.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAKACIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            11829..12464
                     /locus_tag="WIB30_000700"
     CDS             11829..12464
                     /locus_tag="WIB30_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ3796801.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            12501..13469
                     /gene="waaC"
                     /locus_tag="WIB30_000701"
     CDS             12501..13469
                     /gene="waaC"
                     /locus_tag="WIB30_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ3796802.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            13696..14445
                     /locus_tag="WIB30_000702"
     CDS             13696..14445
                     /locus_tag="WIB30_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ3796803.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            14456..15391
                     /locus_tag="WIB30_000703"
     CDS             14456..15391
                     /locus_tag="WIB30_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ3796804.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAATKLGKVDLLVAGNGASAVV
                     EFAKQVAGVKKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQVDSEKLVLTFRATAFDAAPVQGGH
                     AEAVNVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            15454..16065
                     /locus_tag="WIB30_000704"
     CDS             15454..16065
                     /locus_tag="WIB30_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ3796805.1"
                     /translation="MITTYKTITSLTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(16117..16434)
                     /locus_tag="WIB30_000705"
     CDS             complement(16117..16434)
                     /locus_tag="WIB30_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ3796806.1"
                     /translation="MQKRIEEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            16592..17863
                     /gene="purD"
                     /locus_tag="WIB30_000706"
     CDS             16592..17863
                     /gene="purD"
                     /locus_tag="WIB30_000706"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ3796807.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            17897..18499
                     /locus_tag="WIB30_000707"
     CDS             17897..18499
                     /locus_tag="WIB30_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ3796808.1"
                     /translation="MTNVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         4578 a         4212 c         5268 g         4818 t
ORIGIN      
        1 tctgtgccgc tgccgaccga tgtcgtcgtt gccaaagcct ttaccgctga tgaggaagcg
       61 gtcgtgaaag acattgccga cgttgccgaa gacgatatga ttttggacat cggcccgaaa
      121 tccgccgccg agcttgccga tttgctcaaa gctgccggta cggttgtatg gaacggccct
      181 gtcggcgtgt ttgagttcga tcaattcgca ggcggcacga aagctcttgc cgaagccatt
      241 gcccaaagca aagcattctc gattgcaggc ggtggcgaca cgctggcggc gattgctaaa
      301 ttcggcgtta ccgaccaaat cggctatatt tctaccggcg gaggcgcgtt tcttgagttc
      361 ttggaaggta aagagttgcc tgccgtagcc gctttggaaa aacgcggcgc gtaagcggtt
      421 tgatataaaa aatgccgtct gaaacgttgt gctgtttctg acggcatttt tgtgtggcga
      481 ttattgtgct ttggctgccc attccgccgg agtggcggca accgaaatgg aaagtgcgtg
      541 cagttcgtta ctttccagtt gggctttgag tccgtcttta atcaggcggt ggcgcgcgag
      601 gcgtgccttg ccttcaaatt ctgatgaaac gatgacggcg aaaaaatggt gtccgtcgcc
      661 ttctacttcg atatgttcgc attttgccac gccttcaatc atggctttta cttgttccga
      721 ggtcagcatg gtaggttcct tgttggaaaa atgcgccatt atactggcaa attgaaatgc
      781 cgtctgaaaa cgggttcgga cggcattttt gacggtgttt tattttttgg ctttgaagcc
      841 gagcaggtgc tgggtggtgg caagccacgc gccgaatacg cccaacgcga tgacgaagcc
      901 gaacaccagc gcgatttctt caggataaaa gaaacgccag ccgatattga gcccgtaggg
      961 tttgaagatg gcatcgacca aggggcgcac tgtggaaagc agccaaccgc aaagccccag
     1021 gctgatggcg gcggaaagga tgctctgcca cattgcctga taaaggaagg ggcggcggat
     1081 gaacgatgcg ggcgcgccca agagtttggt gatttcgatt tcttctttgc ggctgaggat
     1141 ttgcaggcgg atggtgttgt gtgcgacaag gacgaacgcc atacccagcg tcagggaaag
     1201 aaaccataaa attttacgga taaattcgtt gatttggtac agcgtttgca cccattcggt
     1261 atccatagac gcggattcga ccataggcag tttggtaatg tcccggtaga ttgcctgcat
     1321 ttgcgcgggc gttgtcgcgg ggtcgggggt aacgataaat gcgtcgggca gcgggttgcc
     1381 gtcgagcatc gaaaccaaat tttggtcgag gttggactgt aattccgcca agccgtcttc
     1441 cttgccgatg aagcggacgt tggcgatccg tttgtcgcgc gccagcaggc tgcggacggt
     1501 atcgctgtcg ctttgtgcgg cggcggtttc catatagacg gtgatttgcg gcgactcgtt
     1561 gagtttgccc aacacgcttt gcccgctttg aatgcccaaa tacataaaca gcggcagggt
     1621 catcgcgacg gcgagcatca ggagggtgag cagcgtgccg aagggttggc gcagaagctc
     1681 tttgagcgcg gtgcgggcgg attcgatgtg cagcgaggcg tagtggatga tgctcatgcg
     1741 agtcgtcctt tcgagaggcg caggatgcgg tgtccgtagt ccgccatcag ggtttcgtca
     1801 tgggcggcaa cgatgacggt tgttcccgct tcgtggaagg ttttgaacaa ttccatgatg
     1861 tcgagcgcgt aggcgcggtc gaggttggcg gagggttcgt cggcaatcag caggccgggc
     1921 tggtgaacga cggcgcgggc gatgcacagg cgttgttgtt caccgccgga gagggttacg
     1981 ggatcgtcca attctcgtcc tttcaggccg actttttcga tggcgatgcg ggcgcgttct
     2041 tcggctttgc gcggcggata gccgataatc cgaagcggca ggatgacgtt ttgcaggacg
     2101 ttgcggtcgt agaggatttt gtggtcttgg aacacgatgc cgatgtgttg gcgcataaag
     2161 ccgatttggt tgtcggacaa tgtgccgagg tcctgcccgt taaacaggat tttgcccctg
     2221 ctcggcttgg tgatgcccga aatcagcttg aggacggtgg atttgcccga accggagtgt
     2281 cccgcgataa aaatcatctc gcctttgttg atttggaagc tgacgttttt cagggcttca
     2341 aaaccgccgg gataggtttt ggaaacttgt tcgaaacgga tcataggagg tcctgtaagg
     2401 aagggcggca tatcgtttcg ggcggttgcg tccgcaggga tatgccgtct gaacaatagg
     2461 taagacagtg ccgattataa agattccgcc tgcttttaga tagtggataa gtttggcgga
     2521 tatgaaggtc gggcgggaaa aatgccgtct gaagcggctt cagacggcat ccggcgttta
     2581 acggcatttt gaatgggcga gtttgacggc ttcggtcagg cttttttcgt tcacttcccc
     2641 ggtaatggtc tgcctgtatc cgcatttcgg tgcttcgacg acggtaaagg gcagtacgcc
     2701 gacagtgttt ccgtaggttt tcataaagtt tcggctgttc gccccggtgt aacgccaaat
     2761 cgggtaggaa acaggagttt gtttgaggaa gttgccgata ttgtcggatg tgtcgagcgc
     2821 gatgccgacc atatcgacgc tgcctttttt ctgcgctttg taccatttgg acatggcggg
     2881 catctctttt cggcacgggc cgcaccaagt cgcccacagg ttgacgatgc gtacgggggc
     2941 tttgagcgat tgcaggcttt gcgcggtacc gtctttccat ccggcaagtt cgtctgccga
     3001 ggcggtatgt gcggcaaatg tggcggcaag ggcgattgcg gcaagattca agtatttcat
     3061 cgttttgtcc tttgtatgtt gattcgggta ttgtaaaccg tttggtacgg ttgggggcgg
     3121 gatattgagt aaaaccctgc gtaagccgat aataagggga ttgttgagaa gcaaggaaat
     3181 gtcatgcctg aaatcaaaat tttccacaat ccgcgttgca gcaaatcgcg tgccgccttg
     3241 tccctattgg aagaacgcgg cattgctgcc gaagtggtca aatatttgga tacgccgccc
     3301 gacttgtccg aattgaagga tattttcaac aaattgggct tggcatcggc gcgcgggatg
     3361 atgcgtgtga aagatgattt gtacaaggaa ttgggtttgg acaaccccga tttggacaat
     3421 gacgcgctgc tgcgtgccat cgccgagcat cccgccctgt tggagcgtcc gattgttttg
     3481 gcgaacggta aggcggctgt cggcaggcct ctggaaaata tcgaagcgat attgtgaaca
     3541 aacggctgtt ttgcagccgc aacggcttgc gctattacct gttgggcggt ttctgtttga
     3601 gtgtttttcc gcttttgctg gtgtttgcct catccgtttg ggcggtttat cggacgggcg
     3661 ggcaggtttt gccgccgtat gtccgggcgg atgccgcgct ggtgttgggc gccgccgcgt
     3721 gggacaaacg cccttccccg gttttccgcg aacgcatcaa ccacgccatc gcgctttatc
     3781 agagccgcag ggtaggcaaa atcgtgttta cgggagggcg aaccaaaaaa ggctatatga
     3841 ccgaagccga ggtcgggcgg cggtacgcgc tcaaacaggg ggttccggcg cgcaacatcc
     3901 tgtttgaaaa cacttcgcgc aacacttatg aaaacctgaa caacatccgt ccggttttgc
     3961 gtgccaacgg tattgacagc gtggtgattg tcagcgatcc ctaccatttg gcgcgggcgg
     4021 cggaaatggc ggaggatttg ggtgtccgcg tttatatgtc tgccaccccg accacgcggt
     4081 ttgatgcggg aaataagaaa aaaatcttta tgctgcaaga gggatatgcg ttatccctct
     4141 accgtttgga aaaatggggc agccgctttt tgggatggct gtctgattga agcggtagaa
     4201 aatgccgtct gaaacttatc cgcaggcagg gttcggacgg catttttgat gcccaagcac
     4261 ggtaaaacca gtaaaataaa cgctttcaaa gatttcggag ccatcatgac cgtcaaaacc
     4321 cgtttcgccc ccagccccac cggctacctg cacattggcg gcgttcgcac cgccttgttt
     4381 tcgtgggcgt ttgcccgcca tcataaaggc gaatttttat tgcgcatcga agacaccgac
     4441 ttggcgcgtt ctaccgccga atccgtcaac atcatcctcg acggcatgaa atgggtcggc
     4501 ctgaactacg acaacgccga caatgtcgtg taccaaaccc gccgtttcga ccgctacaaa
     4561 gaagtcattg ccgaactttt agaaaaaggc catgcctact actgctattg cagcaaagaa
     4621 gagctggaag ccatgcgcga gaaagccgaa aaagaaggca cggcgactta cgaccgccgc
     4681 tggcggccgg aagcgggcaa aaccctgccc gaaatccctg ccggcgtgca acccgtcgtc
     4741 cgcttcaaaa cgcctttgga tggcgtaacc aagtgggcgg acttggtcaa aggcgaaatt
     4801 tccatcccca acgaagccct cgacgacctg attatcgcac gcgccgacgg cacaccgacc
     4861 tacaacttct gcgtcgtggt ggacgactac gacatgggcg ttacccacgt tatccggggc
     4921 gacgaccatg tgaacaacac cccgaaacaa atcaatatct taaaagccat cggcgctacc
     4981 ctgcctgaat acggccacct gccgatgatt cttaacgaac aaggcaaaaa aatctccaaa
     5041 cgcagcggcg ataccgttgc cattaccgat ttcggcgcga tgggcatcct gcccgaagcc
     5101 atgctcaact atctggcacg cttgggctgg gcgcacggcg acgacgagtt ctttacgatg
     5161 gaacaattca tcgaatggtt tgatttgaaa gacgtttccc cgtccccaag ccgtatggat
     5221 ttgaagaaac tctactggat taacggcgaa cacatcaaaa tcaccgccaa cggcaaactc
     5281 gccgaactcg tcaaaccccg ccttgcgctg cgcgatattc atgaaaccga aaaacctgcg
     5341 ttggaagacg tattggcact ggtcaaagac cgcgcccaag atttgaacgc cttggcagac
     5401 gaatgtctgt atttctacaa aaaacaagtc cctgccgaag ccgatgttgc caaacactgg
     5461 gacgacgaag ccgccgcccg tatgctgcgc tttgccgaac gcctcgaagg gctggaagac
     5521 tggaatgccg aagccatcca cgacctcttc aaacccttct gcgacgaaga aggcattaaa
     5581 atgggcaaac tcggcatgcc cctgcgcctt gccgtctgcg gtacggcgaa aacccccagc
     5641 gtcgatgctg tgttggcatt aatcggcaaa gaagaagtgt tgaaacgcat ccgcgcttaa
     5701 gttatccaca ggttcgggta atgccgtctg atgttggaat acggcttcag acggcattta
     5761 aatggaagtt aaaaatgaaa aaaatatttt attttctgat ggttgttttt tctaccagtg
     5821 tatgggcagg ggatgctgaa gacaatttgc tcagtatcca atccggttac cgcgccttat
     5881 tgcaaaaaca aaacaagctt gacggaaaaa ttatcgggat gcagtcggat ttggaagatg
     5941 cgcgcagacg gttgcaggcg gctcaggcag acatcgcccg tttggaagcg gaaattcctg
     6001 cagcaatggc gcaaaaagcc cggcaggctg aagagctgag gcaaatcgga gtgcgtttgg
     6061 accatgcttg gaatgcggtt tacggcgcag ggggaacgaa agcgtcgggg aattgaccga
     6121 tgaattccct ctttgtggac aatactgttt tcattacacg gctgaaagcc gggcatatcg
     6181 gcaggttggt tcaggcgttg tttgaggagt ggcacggatt tgaaccgtgg tcttctgtgg
     6241 ataagattcg tgcctattac ggcaggtgtt tgaaggatga cgaactgccg ctggcttttg
     6301 cggctgtgga tgatttcgga attctgttgg gttcggctac ggtcaagcgg catgatatgg
     6361 aaagttttcc acagtatgaa tattggttgg gggatgtctt tgttttacct gaatatcgcg
     6421 gaaaaggcat tggcaggagg ttggtcgccc actgcatagg cgcagcgcgt tcgctgggga
     6481 taaagttctt gtatctttat acgcctgatg tgcaaatatt ttatgaatca ttcggctggg
     6541 cggttgtcgg gcgacatttc cataacggtg aatgggttac ggttatgcgt ttggatatag
     6601 ataaggttta agccgaatgg acaaatgccg tctgagctgt tcggacggca ttttttatat
     6661 ttggaaattt cctgtcagat aatgtttatc cacagatttt tccatatggt ttgatgctgt
     6721 tccgacaacg gaaattaaga tacggatttg cacaaatctt tcagacggta cagttctgac
     6781 aatgcttcgc gcggggtcag gctgtcggga tcgagttttt ccaaggctgc tgccaatata
     6841 ccttcaaaat gtttttcctc tgctttatcc acaaagttgc ccacattcgg ttcatctcct
     6901 ttttcagacg gcatggtact gaaaatatcc agttggggac ggtttgcggc ggcttggtct
     6961 tccagttcgt tcaaatgctt ttgggcggat ttcaatgcgc gtacaggcag gccggcgagt
     7021 ttggcgacgg cgatgccgta gctttttccg gcgggccccg gttggatttg gtgcaggaac
     7081 acgatgtcct gtccctgttc gagcgcggaa aggtgcatat tgacggcggc ggcgtgggtt
     7141 tcgggcaggt aggtcagctc gaaatagtgg gtggcaaaca ggctgaagga tttgtttttt
     7201 tgcagcaggt gttcggcaac ggcgtgcgcg agggcgaggc cgtcgaaagt ggaagtacca
     7261 cgtccgactt cgtccattaa aacaaggctt tgttcggtgg cgtgatgcag gatgtaggcg
     7321 gtttcgctca tttcgaccat gaaggtggag cggttggagg cgagatcgtc cgatgcgccg
     7381 atgcgggtga agatttgatc gatgggtccg attgtggcgg catcggcagg cacaaaacag
     7441 ccggtgtgtg ccaataaaac aatcagcgcg acttggcgca tgtaggtgga tttgccgccc
     7501 atattggggc cggtgagcag catgaggcgg tgtttgtggt cgaggtcggt gtggttggcg
     7561 gtgaagtggc gtacctgctg ttcgacaacg ggatggcgac cgttttcgat gtggataacc
     7621 ggatagtcgg caaactcggg gcggacgaag ttccgctctt ttgccaaggc tgaaaatgtg
     7681 gacaacacgt ccagcgcggc ggcggctttg gcggcttttt gaagctgcgg caatgccgtc
     7741 tgaaggtttt tcaatacgcc gtcaaagagt tgtttttcta aggcgagggc ttgctcttga
     7801 gcagtcagca ctttgtcttc aaaggctttc aactccggcg tgatgaagcg ttcggcgttt
     7861 ttgagggttt gccggcgttg gtagtcggca ggtgcttgtt cggcttgggt tttggacaat
     7921 tcaatgtaaa agccgtgaac gcggttgaac tcgactttga gtgtggacaa accggtacgt
     7981 tcgcgttcct tggcttccaa atccagcaaa aattcgtcgc catggttttg aatgcggcgc
     8041 aattcgtcca gttcgggatg aaaaccgtgg ttgatgacat tgccgtcttt cagccagacg
     8101 gaaggttcgg gcaaaatggc ttggcggagc tgttcggctg tggataggtt ttccgggaaa
     8161 acggctttga gggtttccaa caggctgctg ccgtttgcgg acaagtcgat ttgtgccaac
     8221 tcgaacagac tgtcgcgtaa agatgccaaa tcgcgcgggc gggcgttgcc gacggcgatg
     8281 cgggcagcaa tgcgttcaat gtccgcaatg tttttcagac ggcattggag gggtttgtat
     8341 tggctttcca gcgcggcaac ggcttcttgg cgcgctcgga tgtgggcgcg gttgcgtaaa
     8401 gggtggtgca gccagagagc caagaggcgg ctgcccatat gggtagcgca aaggtcgagc
     8461 gtggacatca gggtcggcga ttttttgccg gagagggttt gcgtgatttc gagattgcgg
     8521 cgcgtggcgg catccatacc gatatattgg ctgtcggttt cgagcgacag gccgtccaaa
     8581 tgttgcggca tcaggttttg cgtcagacgg atatagttca acagtgcacc tgccgcgcca
     8641 atcgcaacgg cgtgttcttt gccgtccaaa ccgaagccgc gcaaatcctg gcagccgaaa
     8701 tattccgtca gcagtttttc ccccgcgtcg gcggcaaact gccacgcgtt caggcgcgta
     8761 acacccgatg ccgtctgaag ttgcggtgcg tttttactgt caggcaacag aatttccgcc
     8821 gcctgcaggc gcgccagttc gtcgtccaat ttatccacag ttgtcagctt ggttttgaat
     8881 tcgccgcttt gcagcgatgc ccacgccaaa ccgatgtatt ttttgtcggg ggacacggca
     8941 acgatgcggt tggtttcctt gtcttccagc aatgcggaat cggtcagcgt gccgggcgtt
     9001 acgatgcgca cgactttgcg ctccacaggc cctttgcccg cgccgacttc gccgacctgt
     9061 tcgcaaatcg ccacgctttt acccaacttg accaggcgcg ccagatattg ttcggcggcg
     9121 tgaaacggca cgcctgccat tttgaccggc tcgccatcca cctgtccgcg cgtggtcagg
     9181 gtaatatcca aaagttttgc cgcttctacc gcatcgtcga aaaacatctc gtaaaaatcg
     9241 cccatacggt aaaacaccag tttgtcggta tgttgcgctt tgatgccgag gtattgctgc
     9301 atcattgggg aaacggcgga tttggacatg gggctgcctt atcattagga aaacggtatt
     9361 gtagcaaaag gttttgtctt cgacggaatg agactgaccg taagcctgtc aataaggttg
     9421 tggagacaac taggaaaaca gcaaagttat ccacagaaat attattaagt caaaaaacat
     9481 ccacgcttat ctgtcttata cagcaagtct taaactcaaa cttatttgac aaaaaggtaa
     9541 tgaaaatatt ggatatttgc tacttacgca caagcaggtt ggcaatcatc aaaatcatca
     9601 ttaaaatttt aaattcaaat attgatggta aaagttttgg aaatctgtgc aaacagtaaa
     9661 attcatctat atcaaataag cggcaagtag ttagatgtaa tagtggaatg taactttatt
     9721 tcagaataca atacaggtat tgaaatttcg tttcacagct tggagtatat acatgagcat
     9781 taaagtagcg attaacggtt tcggccgcat cggccgcctc gcattgcgtc aaattgaaaa
     9841 agcccacgac atcgaagtcg tcgccgtcaa cgacctgacc cctgccgaaa tgctgctgca
     9901 ccttttcaaa tacgacagca cacaaggccg tttccaaggt actgccgaat taaaagacga
     9961 tgccatcgtg gtaaacggca aagagatcaa agttttcgcc aatccgaatc ccgaagaatt
    10021 gccttggggc gagttgggtg tggatgtcgt cctcgaatgt accggcttct ttaccaataa
    10081 aaccaaagcc gaagcacaca tcagggccgg cgcgcgcaaa gtcgtgattt ccgcacccgg
    10141 cggcaatgat gtgaaaaccg tcgtatatgg cgtaaaccaa gacattttgg acggcagcga
    10201 aaccgttatc tccgccgctt cctgcaccac caactgcctt gccccgatgg cggcagtcct
    10261 gcaaaaagag ttcggcgtag tcgaaggtct gatgaccacc atccacgcct acaccggcga
    10321 ccaaaacacc cttgacgcgc cgcaccgcaa aggcgatttg cgccgcgccc gagccgccgc
    10381 gctcaacatc gtgcccaaca gcaccggtgc cgccaaggcc atcggtctgg ttatccccga
    10441 attgaacggc aaactcgacg gctccgccca acgcgtccct gttgccaccg gctcgctgac
    10501 cgaattggtt tccatcctcg aacgccctgt aaccaaagaa gaaatcaacg ccgcgatgaa
    10561 agcagccgcc agcgaatctt acggctacaa cgaagatcaa atcgtttctt ccgacgttgt
    10621 cggcatcgaa tacggttcgc tcttcgatgc gacccaaacc cgcgtgatga ccgtgggcgg
    10681 caaacaattg gtgaaaaccg ttgcctggta cgacaacgaa atgtcctaca cctgccaact
    10741 cgtccgcact ttggaatact tcgcaggcaa aatctaaggc ttgaacaaac ctgtggataa
    10801 gtaaatgccg tctgaacaca ttgcgttcag acggcattgt ttatctgtat agtggattaa
    10861 atttaaacca gtacggcgtt atctcgcctt gccgtactat ttgtactgtc tgcggcttcg
    10921 ttgccttgtc ctgatttaaa tttaatccgc tataacttga tattgcggct tatggcgaac
    10981 aaacaacagc cgtttgttca actgctatgc ggcaaaaccg gatttttgcc cgatttaaag
    11041 cggggttggg gaaagatcaa taacggttgc acggttttcc tcgatttgaa atctgacttc
    11101 gtaatctgca atattcatca cataaatccg ttcgggaata ttctgatagg cggggcgcgg
    11161 atcttgggca atgctttggc tgataaggtt tttggtgttt gcagataaat tttccgcgcc
    11221 gatgttttcc tgccaaacga cttccaactc tacgggtttg ccgctgacga aaccggatgc
    11281 ggcatcgggt ttggattcga caaaggggat ataaggtttg atgtccacaa tcggtgtgcc
    11341 gtccagcagg tctgcgccgc tgcaatagag gcggacgggt ttgccggttt cgatgcgttc
    11401 gagtttcagg agcgagagtc cgagatggtt ggggcggtgg gggctgcgcg tggcgaacac
    11461 gcccattttt tgtttgccgc cgagccgtgg cgggcgcacc atttgcgccc agccttcatc
    11521 caatacgccg tgaaaaataa aacttatcca cacataatcg aaatcttcca gcccgcgcac
    11581 gctgtctgcg gtgaatttgg gattcagctc gatgcaggct tttgcggcgg agaccaaacc
    11641 gggctggcgg gcgatgccga atttctgttt gtagggcgag cgggcggtgc cgatgggggt
    11701 aatggtataa gtcataaggt tgtggataag ttttttggaa aaaccttatc ttatcacaat
    11761 cggttttacc gcttttgggc agtaatttgt tataatacag ggcattttaa tcgcgttgaa
    11821 ataatgagat gattgtttcc attgatgttg atgcacaaaa aacgtttacg ccactgtgtc
    11881 ctgacgaact gcccgtgaac gaggggcatt tgattgtcga ggagttgaat gcgcaagccg
    11941 ctttggcgga tttgcgcgtg atgacgaaag atgcgcatca tatggtagcg aaatggcttg
    12001 tggataaccc tgttgatatg ttgaagccga caggtttgcc tgatgcggat ttgacttggg
    12061 tggctcatgc gatggtcggt acgcgcggct atgaattatt ggacgggctg ccttctgcta
    12121 aagaatacga ttattgcgtt tggaaaggtg ttgatcctga attgcatcct tacggcgcgt
    12181 gttttcacga tattgaggaa aaactaagca cagggctgat tgaatggctg cgttgtcaaa
    12241 atacggatac ggttattatc ggtgggttag ctacggatta ttgtgttaaa acaacggttt
    12301 tgcagttact caaaggcggt cgttggcagg ttgtcgtcaa tgaagcggct tgtcggggta
    12361 ttgcgccgga aaccatcgaa acagcatggc aggaaatgca ttctgctggg gcaatcatct
    12421 taaaaaacgc tgatgaaata aataaatata ttaataatca ataagttaaa attgtttcac
    12481 gtgaaacccc cttcctaaat atgaaaattt tgcttgtccg cttgtctagt atgggcgatt
    12541 taattcacac tttgcccgca atcgaagatt tggcgcgaca atgtcctgat gtagaactgc
    12601 attggctatg tgaggctgga tttgcagata ttgcgcgcct gcatccgttt gtaaaaaaaa
    12661 tccatgtgat gaaatggcgg caatggcgca aacatctctt tcgggctgaa acttggcggg
    12721 aaatgggtca tctgaaacag actttgcggc aggaagtatt tgatttcgta ttggacagtc
    12781 aaggtctgat taaaagcgcg tgtttcgcta aaatggcaaa atccccgatt tgtggtttgg
    12841 ataaaaacag tgcgcgcgag ggatgggctg ctttggcgta tgtaaaaaca tacgctgtac
    12901 cgaagggaaa aaatgccgtt tggcgcaacc gtgaactgtt tgcccaagta tttgggtatg
    12961 taatgccgga aacgcaggta tttggcttga ctgttcctga agcaggccgt ctgaaaaatt
    13021 tagcgcagcc gtattatgcg gctttgcatg cgaccagtcg ggacagtaag ttatggcctg
    13081 tggaaaactg gcggtcgttg ctgcaaaagt tgaatgaaga acagcaatgc aatatttacc
    13141 tgccttgggg aaatgaagat gaaaaaacgc gtgccaaaca aattgcagat gggctgccgt
    13201 ttgctattgt gtgcgacaaa ataaatttat tgcaggcagc gtatctgctg aaatacgcgg
    13261 tcggaattgt cggcgtggat accggtttgc tgcatttggc aaatgccttg gaaaaacctg
    13321 tggtcggcat ttataccgat accgatccga ttaaaacagg cgttcaggtt tcgccaattg
    13381 caaaaaattt gggcaatatc gggcagattc cgaccgcaga tttggtttat caagcgctga
    13441 tggattgtgt tgcagcagat aaaggctaaa gggtcgtcta aaactgatat ggcggcatga
    13501 tattttccaa tttattagat acaggaatgt atatccgcta gtctgagtaa atgctttaaa
    13561 aatggtataa tcggcaagca ttatccgaaa gatttttgaa tgaattcgtt tttaaggaaa
    13621 acggatttgg ttttataggg aagaaggttt ggtttcgctg gatggtgaaa gcagcgttct
    13681 ttttaggagg gttcaatgaa agcactggtc gcagtaaagc gcgtagtgga ctacaacgtc
    13741 aaagttcgtg taaaagccga tggttcggat gtggatatcg gcaatgtcaa aatgtcgatg
    13801 aatccgtttg acgaaatcgc tgtggaagaa gctgtccgtt tgaaagaagc cggaaaagta
    13861 agcgaaatcg tagcggtttc tttgggtgag aaaaaatgcg aagaaacctt gcgtacagct
    13921 ttggcgatgg gtgccgaccg tgccattcat gttgaaaccg atactaaact ggagtctctg
    13981 gccgttgcta agttgctgaa agccgttgcg gacaaagaaa atccgcaaat tttctttttg
    14041 ggcaaacaag cgattgatga tgatgccaat caagtggcgc aaatgctggc agctttgctg
    14101 aatgcggcgc aaggtacgtt cgcgtccaaa gtacaaattg aaggcgacga agtgcagatt
    14161 gtgcgggaaa tcgatggcgg cgaagaaacc atcgcattga aactgcctgc tgttatcagc
    14221 gcggatttgc gtttgaacga gccgcgcttt gtcaaactcc ccaatattat ggcggcaaag
    14281 aaaaaacctt tggaaaaact gactcctgac gatttggttg ccgacatttc acctcgtttg
    14341 aaaacggtga aatttgccga acctaaggcg cgtcaggcag gcgtaaaagt agcaagcgtt
    14401 gccgaattgg ttgaaaaatt gaaaaacgaa gccaaagtga tttgaggagg ctgaaatgag
    14461 tgtattgatt attgtcgaac acgacaacaa acagttgaat cctaccactt tgcatgctgt
    14521 taccgctgcc accaaactgg gcaaagtcga tttattggtt gccggaaacg gcgcatctgc
    14581 tgtagtggaa ttcgcgaagc aagtagcggg ggtgaaaaaa gttttggttg cagatgctgc
    14641 tcattatgcc gaaggtttgg ctgaagagtt ggctccgctg gttgttaaat tggcggccga
    14701 ttaccgctat gttgcagcaa cggcaaccac attcggtaaa aaccttttgc cccgcgtagc
    14761 agccttatta gacgtaccgc aaatttctga tttgaccgaa attgtggata acacgacttt
    14821 tgtgcgcccc atttatgcgg gtaatgcatt tgaaaccgtg caagttgatt cggaaaaatt
    14881 ggtgctgacc ttccgtgcga cggcttttga tgcagctcct gtacaaggtg gtcatgccga
    14941 ggcagttaat gttgaagcaa cccctgccca aaacctgagt cgttttgtta accgtcagct
    15001 ttcccattcc gatcgtcccg aactgactca ggcaaaagtg attgtttccg gtggtcgtgc
    15061 gttgggtagt gcggaaaaat ttaatgaagt gctgacaccg ttggcggatg ttttgggtgc
    15121 ggcaatcggt gcatcccgtg cagcagttga tgccgaatat gcgccaaacg atgctcaagt
    15181 cggacaaacc ggtaaagtgg ttgcgccgca actctatttt gcaatcggta tttcaggtgc
    15241 gattcaacac gttgccggta tgcaagacag taaggtaatt gttgcaatca ataaagatgc
    15301 tgatgcgccg attttcaatg tagccgatta cggattggtt ggcgatttat ttgaaatcgt
    15361 tccacaatta acagaagcat tgaaaaattg atgtttcacg tgaaactttc agacgacctt
    15421 gttattaatg aggtcgtctg aaaacatttc agcatgatta cgacttataa aaccattacc
    15481 tccttgacgc aggctgagtt taaagataaa ggcagccgtt ttattgcatt tgcctatccg
    15541 attcggacat tggctgatgt gaaaaaatac ctcgatccgt taaaggaaga gcatcataaa
    15601 gcacgacact ggtgctatgc ctatcgtttg ggtgtggatg gtgtgcaatt tcgtgccaac
    15661 gatgatggag agccatcagg aagtgctgga cgaccaattt tgggacagat tgattcggtg
    15721 ggtattaccg acgttttggt cgtggtcgtc cgctatttcg gtggtacttt gttaggtgtt
    15781 ccaggtctga tacatgcgta caaagaggcg acggctcaag cgttggcagt tgcagaagta
    15841 gttgaaaaga atattgaaaa aactgtttgg ctgaaatgtg aatatccgtt tttgaatgaa
    15901 gcgatacgca ttgctaaaca atatcaggcg gatgtattgg agcgggattt gcagttggat
    15961 tgccgattga cggtcagttt gtcgttggta aattatgagg cctgtgttgc ggcttggaag
    16021 agtacacggc agattgaagt aaatacggaa aagccttttg aatgaaaggt cgtctgaaat
    16081 gctttgtgat ggtatttcag acgacctctt cgctgactaa tcttcttgtg tatatttgtc
    16141 taaggcatcg acgctggagc gcaaatataa ccaagccagt tgatggaatt cgcccagcgc
    16201 gttccatagt gcatcttcac tcataatgct gtattcgcct tctgtgcgca aatccacctc
    16261 cgctccatct tcaatagccc gatgacaagc ctgatattcg tgggtataaa aattgtccat
    16321 atcgcgaatc aaagcgacgg catgtttcca acggcggatg tcttcttcca attgcggtag
    16381 taaatgacac cattcgcggt atttgctttg gatttcttca atacgttttt gcatggttgc
    16441 ttgtcctttt ttaatttatg aataagttga ataggttgca gagtggaatg aagcggtctt
    16501 ttgtttgatt ctactaaaat ttcttcagat gggataaaag tccctttgta tcgagttttc
    16561 gttaaaagta catatttgtc aaggagatgg catgaaactg ctggttatcg gtaatggcgg
    16621 tcgcgaacac gcgctggctt ggaaattggc gcagtcgcct aaagtggaaa cagtatttgt
    16681 tgcgtctggt aatgccggta cagcgattga acccaagctg caaaacatcg acttgactgc
    16741 gcatcaggat ttgattgaat tctgtcgtaa agaaaatatt gtttttaccg tcgtcggccc
    16801 tgaagcgcct ttggcggcag gtgttgtgga tgatttccgt gctgcaggac taaaaatatt
    16861 cggtccgaca caatatgcgg cgcagttgga aagttccaaa gatttcgcca aagcatttat
    16921 ggcgaaatac aatattccga ccgcgcaata tcaaaccttt gaaaacgccg atgccgcaca
    16981 tgattacgtc aatcagaaag gtgcgcctat cgttatcaaa gccgatggtt tggcggcagg
    17041 taaaggcgtg attgtggcga tgactttgga tgaagcgcat gctgcgattg atgatatgct
    17101 gctggacaac aaaatgggca atgccggcgc gcgtattgtg attgaagatt tcctgcaagg
    17161 cgaagaagcg agttttatcg tcatggttga tggcaataat gtactgccta tggcaaccag
    17221 ccaagaccac aagcgtcttt tagacggcga caaaggtctg aatacgggcg gtatgggtgc
    17281 gtacagccct gcgcctgtgg taacgcctgt tgtgtacgaa cgcgcgatga atgaaattat
    17341 tttgccgacc gtagcaggga tgaaagcgga ggggcatgag ttcaccggtt tcctgtacgc
    17401 aggtttgatg attgatcaaa gcggtgcacc ccatacgatt gagtttaact gccgtttcgg
    17461 cgatcctgaa acccagccga ttatgagccg actgaacagc gacttatcgg atttggttga
    17521 agcggcaata gacggcaaac tcgatagcgt aaccgcggaa tggagcccgc aaactgccgt
    17581 gggcgtggta ctggcggcgc aaaattaccc cgaaacgccc aaaaaaggcg atgttatttc
    17641 cggtctggat gctgccaacc aagtaggcaa agttttccat gcaggtacaa cggcaaacga
    17701 gaaaggtgac gtattgacca acggcggacg cgtattgtgt gttgtgggat tgggcgataa
    17761 tgtggcgcaa gcgaaagcta aggcatatgg cgcgttggaa aaaatcagtt tcgacgggat
    17821 gcaataccgt aaagacattg ccgataaagc gattaaccgt taattagaaa gaggatgatg
    17881 ccgtctgaag ccaagtatga ctaatgtgct tcagacggta ttttatatta tgttatttcc
    17941 gagaattatc gcagcatcag cgcaacgtaa gttaagcgtt tatttgaaaa aaggtggttt
    18001 ggtcgcttat ccgaccgaat cttgttatgg cttggggtgt cttccgacat tagccaaagc
    18061 actcggtaag ttggcgcatt tgaaaaaaag accgcaacac aagggtatga ttgtcatcgg
    18121 gaatcaattt gaacaattgc agcctttact tcaaatgccg tctgaaaatc ttcaggatat
    18181 gcttagaaaa gaatggcccg cccctaaaac gttcctatta tccgccaaat cctgtgtctt
    18241 acccgagtta cgcgggaaac agcgcagcaa gttggcggtc agggttcccg cccatgtagg
    18301 tgcgcgccgc ttgtgtcagg ctttgcaaac gcctttggtc tcgacttcgt gcaaccgcgc
    18361 aggcaaacgc gcgtgtcgga cggagaggga agtcagaagg caattcggac gcgatgtgtg
    18421 gattgttggt gggagaatcg gcaggcagaa atcgccgagt cagattattg acggggaaac
    18481 aggtaaacgt ttgcgttaga tatgggtact tgaatgaaaa ctttttttgc atacacaaag
    18541 aaaaaatttt tcggatagca tttctctaca tgattgttat tgcacagaga taaaggtaga
    18601 aggacgcaac gtctgcttta attttgagga cggttttgtc gttccggaca ataatccgaa
    18661 caatcaagcg ggcaagcatc tgaaaacaga tttttcccgt gttgttttaa tccataaaaa
    18721 tgaaaacgcc gaagatgatt atttgagacc tttgcaataa cataggttac taaaatttta
    18781 tgctcaatct cattttcaaa atgcaaaact tttctgattt ttcctacttt ttgctcaata
    18841 ttaggaaggt tttaggcaat tgaaaatttt ttggcg
//
LOCUS       DAWHRA010000028        18698 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWHRA010000028 DAWHRA010000000
VERSION     DAWHRA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.028"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(73..1290)
                     /gene="nqrF"
                     /locus_tag="WIB30_000708"
     CDS             complement(73..1290)
                     /gene="nqrF"
                     /locus_tag="WIB30_000708"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ3796809.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNNEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLVAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(1304..1897)
                     /gene="nqrE"
                     /locus_tag="WIB30_000709"
     CDS             complement(1304..1897)
                     /gene="nqrE"
                     /locus_tag="WIB30_000709"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ3796810.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(1901..2527)
                     /locus_tag="WIB30_000710"
     CDS             complement(1901..2527)
                     /locus_tag="WIB30_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ3796811.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(2527..3303)
                     /locus_tag="WIB30_000711"
     CDS             complement(2527..3303)
                     /locus_tag="WIB30_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ3796812.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(3296..4528)
                     /locus_tag="WIB30_000712"
     CDS             complement(3296..4528)
                     /locus_tag="WIB30_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ3796813.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVSGKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLSLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(4531..5874)
                     /locus_tag="WIB30_000713"
     CDS             complement(4531..5874)
                     /locus_tag="WIB30_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ3796814.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(6222..7643)
                     /locus_tag="WIB30_000714"
     CDS             complement(6222..7643)
                     /locus_tag="WIB30_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ3796815.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGSLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(8002..8361)
                     /locus_tag="WIB30_000715"
     CDS             complement(8002..8361)
                     /locus_tag="WIB30_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ3796816.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(8358..8963)
                     /locus_tag="WIB30_000716"
     CDS             complement(8358..8963)
                     /locus_tag="WIB30_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ3796817.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(9010..9492)
                     /locus_tag="WIB30_000717"
     CDS             complement(9010..9492)
                     /locus_tag="WIB30_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ3796818.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      9695..9792
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10003..11103
                     /gene="gcvT"
                     /locus_tag="WIB30_000718"
     CDS             10003..11103
                     /gene="gcvT"
                     /locus_tag="WIB30_000718"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ3796819.1"
                     /translation="MTALKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGQGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            11265..11651
                     /gene="gcvH"
                     /locus_tag="WIB30_000719"
     CDS             11265..11651
                     /gene="gcvH"
                     /locus_tag="WIB30_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ3796820.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDSLLTAEQYAGEVD"
     gene            11803..13050
                     /locus_tag="WIB30_000720"
     CDS             11803..13050
                     /locus_tag="WIB30_000720"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ3796821.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            13326..13751
                     /locus_tag="WIB30_000721"
                     /pseudo
     CDS             13326..13751
                     /locus_tag="WIB30_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            13757..14986
                     /locus_tag="WIB30_000722"
                     /pseudo
     CDS             13757..14986
                     /locus_tag="WIB30_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            15044..15664
                     /locus_tag="WIB30_000723"
     CDS             15044..15664
                     /locus_tag="WIB30_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3796822.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLIGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            15675..15806
                     /locus_tag="WIB30_000724"
                     /pseudo
     CDS             15675..15806
                     /locus_tag="WIB30_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            15803..16297
                     /locus_tag="WIB30_000725"
     CDS             15803..16297
                     /locus_tag="WIB30_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ3796823.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(16385..18046)
                     /locus_tag="WIB30_000726"
     CDS             complement(16385..18046)
                     /locus_tag="WIB30_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ3796824.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEVGAHSLAGAVIDPIALNELISDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(18176..18685)
                     /locus_tag="WIB30_000727"
     CDS             complement(18176..18685)
                     /locus_tag="WIB30_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ3796825.1"
                     /translation="MVKQDLDFSCGAASVATLLNNFYGQKLTEEDVLKKLDKEQMPAS
                     FEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVLRGIDGNTVLLA
                     DPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFTRSPKRQTEFAV
                     GQVKWWRAY"
BASE COUNT         4582 a         4789 c         5030 g         4297 t
ORIGIN      
        1 ggtgttcgga cggtatcgga taaaaaaagg aagaaaccga ggtttcttcc ttttgtgttt
       61 gaagccgaat atttaaccgc cgaaatcgtc caagaggatg ttttcgtctt ccacgcccaa
      121 gtctttgagc attttgatga cggactggtt catgattgga ggaccacaca tataaaattc
      181 gcagtcttcc ggtgcttcgt gatttttcag gtggttttcg taaaccacgt tgtgaatgaa
      241 gcctgtgtag ccgtcccagt tgtcttcggg caatgggtcg gacagggcga cgtgccacgt
      301 aaagttcggg aactctgcca cgagttggtc gaagtcttcg acatagaaca tctcgcgttt
      361 ggaacgcgca ccataccaga aggtgatttt gcgtttggag ttcaaacgtt tcaactggtc
      421 gaaaatgtgg gaacgcatcg gcgccatacc cgcaccgccg ccgataaata ccatttcggc
      481 atcggtgtct ttggcgaaga attcgccgaa cgggccggaa atcgtaactt tgtcgccggg
      541 tttgagcgac cagatgtaag aagacatttg tcccggaggc gcatcgggta cgcgcggagg
      601 cggcgtggcg atacgcacgt tcagcataat gatgcctttt tcttctggat atgaagccat
      661 agagtaggcg cgcaaaatcg gctcgtccac tttggaaacg tattgccaca aattgtattt
      721 gtcccagtct tcgtgatatt ccttaggaat gtcgaagtct ttgtaggcaa cagtgtgagg
      781 aggagcttca atttgaatgt agccgccggc gcggaagggg acttcttcgc cttcgggaat
      841 ggcaagcttg agttctttaa tgaacgtggc tttgttatcg ttggagatga cggtgcattc
      901 ccattttttc acgccgaaca cttcttcggg gacttcgatg tccatgtcgg ttttgacgtt
      961 gacttggcac gacagacggc agccttcgcg tgcttcgcgt ttgctgatgt gggacagctc
     1021 ggtcggcagg atgtcgccgc cgccgctttt tacgacgacg cggcattgtc cgcacgaacc
     1081 gcccccgccg caggcggagg ggataaagat gccttcgttg gcaagcgcgc ccaagagttt
     1141 gccgccggcg ggcatcgtca gctctttttc gttattgact ttgatggtga tgtcgccttc
     1201 gctgaccagt ttggatttgg caaacagaat catcagtgcc aaaaccaaaa cgatgacggt
     1261 aaacatcacg atacctaaaa taatctccat accgatccct ttcttataac tggatgccgg
     1321 agaacgacat aaacgccatc gccatcaggc cggcggcgat aaaggtaatg cccaagcctt
     1381 tgaggccttt gggagcgtcc gaatatttca ttttttcggt aatgcccgcc aaagcgacaa
     1441 tcgccaacat ccagcccaag cccgcgccga aaccgtatac aacggactcg ccgaagttgt
     1501 attcgcgttg cgccataaac gatacggcgc cgaaaatcgc gcagttcacg gtaatcagcg
     1561 gcaggtagat gcccaacgcg ttatagaggg cggggacgaa tttatccaag aacatttcca
     1621 aaatctgcac caaagcggca atcacgccga tgaaggtgat gaatttcaaa aaggtcaaat
     1681 ccacgccttc gacaatcgcg ccgtctttga gcagcgagta aacgagttgg ttgacaggga
     1741 cggacagccc gagtacgaaa attaccgcca cacccaaacc gaatgcggtg gatacttttt
     1801 tggataccgc caaaaacgtg cacataccca aaaagaagga tagtgccata ttttcaatga
     1861 agacggattt gatgaagagg ctcaaatagt gttccatagc ttattcctcc gcctgttcgg
     1921 gtttccaggt acgcagtccc caaatcaaaa agccgatgat gaagaacgcg ctgggggcga
     1981 gcaggaacaa gccgttggtc tgataccagc cgccgtcctg cacggtttgg aaaacggtgt
     2041 agcccaagag tttgcccgag ccgatcagtt cgcggacggt ggcgacgaca agcagcatca
     2101 tcccgtagcc cgcgccgttg ccgatgccgt cgatcaggct ttccagcggc ggctctttca
     2161 tcgcaaatgc ttcggcgcgg cccatcacga tacagttggt aataatcaga ccgacgaata
     2221 cggaaagctg tttggacaat tcgtaggcaa atgcctgcaa gagttggtcg accagcgtaa
     2281 ccagcgacgc gataatcgcc atttgcacga tgatgcggat gctgttgggg atgtagttgc
     2341 gtaccagcga aatgaagaag ctggaaaaac cggttaccaa agctacggaa atacccatca
     2401 cgatggccgt ctgaagtttg gtggtaaccg ccaaagccga acaaataccc aaaacctgca
     2461 aggcaatcgg gttgttgtcg ataaagggtg aaaacatcaa atgtttcaag cgtttcatat
     2521 cagccattat tgtgctcctg ctgatttcaa tttgttcagg taggggatat agccgttttc
     2581 gccgaaccag taggcgaacg aaccttgcac gcctttggaa gtcagcgacg cgccggagag
     2641 ggcatctacg ccgtgttctt tgtccgaacc cgcgcctttg ccgacgtgca gggcgagttt
     2701 gccttgtccg tcaaacagtt ttttgccgac gaatttttgc tgccacaaag ggttgccgat
     2761 ttcgccgccc aagcccgggg tttcgccttg ctcgtaatag gtaatgccgt tgatggtgtt
     2821 gccgtcgggt tggatggcga caaaaccgta catcaccgac cacaaaccgt tgccgtgcat
     2881 aggcaggatg atttgcccga ttttgccgtc ttcacctttt accaaataaa cctcagtgta
     2941 tttggcacgg cttttgatgc ctgccaaatc gtcttcgggt ttgatgcgga tgctttgggc
     3001 agggtctttg cctgcgatgc gcgcgctgaa atctttaggc gcatcggcga cgtattcgcc
     3061 ggtcgccaaa tcgacaacac gttgctcgat acgctcggca aaggttttac cgatgtcggt
     3121 gtccttatcc atcaaaccgg ctacgctcaa gatatagcct tgtttgtctt ggagtttttg
     3181 tttctcttgg atgggtttca agccaacgac cgcaccggca acgatgaccg agcaaatcag
     3241 gctgaccgcc aacacgacaa tcagcgtgcc gctgaagctg tctttatcga atttcttagc
     3301 cattgctgcg cgcctttctg cgtttgatgt tcgcttgtgc gacgaaatag tcgaaaatcg
     3361 gggcaaacag gttggcaaac agaatcgcca acatcatgcc ttcggggtaa gccggattga
     3421 ccacgcggat caatacgcac atcacaccga ttagcgcgcc gtaccaccat ttgccgacat
     3481 tggtaaagga agcggaaaca gggtcggttg ccataaacag cataccgatg gcgaagccgc
     3541 cgacgaccaa atgccagtac caaggcatag caaacatggc gttggtgtcc gaaccgatga
     3601 agttgaacag cgaagacatg gcaatcatac cgatcatcac gccggcaata atgcgccaag
     3661 aagcgatgcg ggcaaacacg ataaacgcgc cgccgattaa gagtgccaaa gtggagactt
     3721 cgccgatgga gccgggcagt ttgccgataa acgcatccat ccaagtgatg gtttgaccgg
     3781 ttacggcgtt tttcaggccg tctgcaccgt gtgccgccca ttgcgccagc gcggttgcgc
     3841 cggaatagcc gtcaaccgcc gtccaaaccg catcgccgct caagttggca gggtaggcga
     3901 agaacaggaa ggcacggcct gccagcgcag ggttcatgaa gtttttacct gtaccgccga
     3961 atacttcttt cgcaaccaca acgccgaaag aaatacccaa agctgcctgc cacagcgaca
     4021 gcgtgggcgg aacgattaag gcaaacagaa tcgaggtaac aaagaaacct tcgttgattt
     4081 catgtttgcg cacggtggcg aacaaaactt cccagaaacc gcccacgaca aatacggtcg
     4141 cgtaaatcgg cagaaagtaa atcgcgccga acagcatttt gcccgacaca cccgcttcag
     4201 acgacatatt gatgcccaaa gcgtcggcaa gggtgtaatg ccagtcgttg gcgatgcttt
     4261 gttgcagcaa atcgggcgtt aaggcaccga atgcctgcgc gccgacgttg tacataccgt
     4321 agaacatggc agggaacaaa gccagccaca ccaaaatcat catgcgcttg gagtcgagcg
     4381 cgtcgcggac gtgcgccgct ttgcgcgtta ccgcgccgga tgtgtagaaa attgtcgccg
     4441 ccgcttcgta gagggcatac catttttcat gtttgccgcc cggcaggaag tgcggttcga
     4501 ttttttccag aaaatgtttc aagcccataa tcagccttcc ttctcaatgg tttccagcac
     4561 cttacgcaac agcgggccgt attcgtattt gcccgggcag acgaagctgc acaaagcgag
     4621 gtcttcttcg tccaattcca agcaacccaa tgcctgcgcg ctgtcggtat cgccgacgat
     4681 taaatcgcgc aaaagcaggg tgggcaggat atccaagggc atcacgcgct cgtaagtgcc
     4741 gatgggaacc atagcgcggt cgccgccgtt gacggctgtc gtgaacttga agagtttgtt
     4801 tttcaggaaa tggccgaggg tcgtccgcgt gatggagtat ttgtccggct gcggcgcaac
     4861 ccagccgaac agctctttgc tgcggccttc ttcgataacg gaaatctgat tgtggtagcg
     4921 tcccaaataa tcatgcgcgc cttgtgcaat cgcgccgttc aataccgaac cggaaatcac
     4981 gcggttgtct gcgtcaacca attcgcccgc agtaatttgc gatactttcg cacccaaaac
     5041 ggtacgcaag aggcgtggtt tgttgacttg agaaccaccc aaggcaacca cgcgctcggt
     5101 gttcagacgg cctgttgcaa acaaacgtcc gatggcaatt acatcttgat aattgatggt
     5161 ccaaacggtt ttgttcgcac cgacaggctc aatgaaatga atgtgcgtgc cactcaaacc
     5221 ggcaggatgc gggccgccaa attcatgagt ttcgatgttg gcggcgtttt cagacggcac
     5281 gtctgcgccg gctgccttac aaacatggat tttgcgttcg gtcaaacggc tcaacaccaa
     5341 caggccgcgt ctgaaatcct cggcggcttc tttgataatg accgtagggt cggcagccag
     5401 cggattggtg tccatcgcat tgacgaagat ggcgaacggc tcggcatcga cggcagggat
     5461 tttgctgaac ggacgggtac gcagcgcagt ccacaaaccg gattggatca gattgcggcg
     5521 cacttcttcg ccgcttaagt ttgccaacgc ttcgggcgcg tagcgttcga actcgatttc
     5581 gtcgttgcct tcaacggcaa tcacgaccga ctgaagtacg cgcttttcgc cgcgatggat
     5641 ggcggcgatt ttgcctgaaa ccggcgcggt aaacaccacg cccggattct ttttgtcttc
     5701 aaacagcact tggccttttt tgacggcatc gccttccttg actttcatcg aggggcgcat
     5761 accggcatat tcttcgccaa gcaacgcgac ttcggtaatg acgggcccgt cataaatgac
     5821 ttgctccggt ctgcccgcga tgggcaggtt tagacctttt ttgattttaa tcatatattt
     5881 gcattacttg tgatggttaa ggtaaaaacg gcgtgttttg ataccgtgtc gcgtggcatc
     5941 aaaagcattg aataaattaa tgtagcaaag tgttagattc tatcaggaat tgtacctgtt
     6001 tgtcagattt gctgcttttt tccttgcgga agccgttttt atagtggatt aaatttaaac
     6061 cagtacggcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgtcgccttg
     6121 tcctgatttt tgttaatcca ctataattgt cggaaggggg gatattgatt tgattatgcc
     6181 ggaatttaaa atgccgtctg aatgttcaga cggcatagcg tttacagcag tttgaaaacg
     6241 aaaaagataa gggtatgtac gatgaagacg ggtgtcagga aggcgaccga ccacatcata
     6301 tagccgaaga aagtcggcat cggtacgccg cgctgttcgg caatggcctt gaccatgaag
     6361 ttcggtgcgt tgccgatgta ggtcagtgcg cccatgaata ccgaacccat agaaaccgcc
     6421 agcagcgaat gaaacaggga acccgtcatc aaggcttggg catcgccgcc tgccatattg
     6481 aaaaaaacga gataagtggg cgcgttatcc aagaatgccg acaatatgcc gctcatccaa
     6541 aaatacatca cattaatcgg atgacctgcc gtatcgtgaa ccagcgatac caccccgccc
     6601 agcgcgcctg cctcgcctgc tttcagaatg ctcaggacgg gaaagatggt gatgaagatg
     6661 ccgaggaaga gtttgcccac ttcggcgatg ggttcaaagt tgaattcgtt gcctgcgcgg
     6721 acttgtttgg gcgtgattgc catagatacg gcggtcaatg caatcaggat gacatcgcgg
     6781 acgaggtttt gcagggcgta acggctgccg aggatttcaa atcccgggtg ttcgggtttc
     6841 caaaggccgg acattagaac cgcgccgacc acgcccgaaa gcaggaggaa gttccatttg
     6901 ccgaagatgg cgattttttc gggtttttcc tgttgtgccg gcgtatcttg tgcaatgctt
     6961 tcctgtttga agaaacggtt gtcgatgaaa tagaaggcgg tcaacaggac agcggtgctg
     7021 atcaggacgg gggcgaacat atgtttgacc gtccacatga aatctacgcc tttgaggaag
     7081 ccgaggaaga gtggggggtc gcccaaaggg gtcagaccgc cgccgatgtt tgcaaccagg
     7141 aaaatgaaga agatgacgat gtgcacgcgg cgggtacggt tttggttggc tttcagcagc
     7201 ggacgaatca tcagcattgc tgcgccggtc gttcccatga tagaggcaag tgccgtaccg
     7261 acggcaagca gggcggtgtt gagcttgggt gtgccgttca agtcgcccca aaccaaaatg
     7321 ccgcctgaaa tggtgtacag ggcaagcagc agcaggatga aagggatgta ttcttcaacg
     7381 agtgcgtgtg cgacggtatg gataccggcg gacgcgccaa aaaccaaact gaacgggatg
     7441 aggaagagca atgtccaaaa ggcggtaatt ttgccgtaat ggtgatgcca ggtatgcgaa
     7501 aaaaacaagg gacccaatgc gatagacagc aaaatcaggg caaagggcag gctccacagc
     7561 aggtttaggt ttgcgccgtc caaatctgcg gcgtaaaccg atgcggggaa aagcattagt
     7621 gaaaacaggg gtaggtggcg catcgtgttt cctcgattca agcactgcct tgcgcggcgc
     7681 gcgggagtga tacaggcacc gtgccgcccg gacataggcg gactgtgctg cctgtttgtt
     7741 tttgaaaaaa gttttccgat tgattgtaaa gtaaataatc aggataaacc agtttcccaa
     7801 accggaaggc ggcgggaagg cggattgccg tgcttgggaa tatatgttct ttttgataaa
     7861 taattttatt taaaacaaat aaatatatgt ctttaataaa taatctatcg aaaacgaaaa
     7921 atgaatttat tttaacatat atttgcaatg aaacaggttt gccccacccg tttgtttgcc
     7981 cttatccctt tcagacggca ttcaagattc gggcctgcgc cacatccata tggcgacaag
     8041 ggaacaaaaa accgatgaaa ccgccccgac ccaccagcgt tggggaaact gccaaaacat
     8101 tatcaggcag gatgcggtca tcatactgat ggcgaatatt ttggctttgc gaggcactgc
     8161 gccgttttgt tcccagttat gaaccatcgg accgaaatag cggtgccggt gcagccagcg
     8221 gtgaaagcgc ggggatgcct ttgcccagca ggcggcggag agcagtacga acggcgtggt
     8281 cggcaacagc ggcaaaaaaa tgccgattat ccccaacagc agggagatgc cgccgcaggc
     8341 aattaaaaga taacgtatca ttttgaaata tttttcttat tgtgcggata agggcaggat
     8401 gtgataccga gttttgccca gccttcatgt cccatttttt ccagcagggc gatattgcgt
     8461 tcgaatattg ccgaagcgtc gggaaaggct tgtgcggctt tggcaatgct gtcttcgcgg
     8521 atgaggtgca gcgtcggata gggagaacgg ttggtgtagt tgccgatgtc gtcgggttcg
     8581 gtattctgaa agcggaattg gggatggaac ggtgcgattt ggacgatgcc ttccaagccg
     8641 ttttcgataa cggcggcatc ggcaatgtcg agcatatcgt tgaatacgtc gaaatcgggg
     8701 aatagggtcg ggtgaaccag cagggtggtt tccagttcgg tggcgggtgt attgcccagt
     8761 cgctgcagtt cttcgtccaa gtcttccaaa aaactgtcaa ggtgtttggc ttcgctgatt
     8821 gcgatgcgga caaggttttt aacgtggggg gctttggcaa agggacacag gttcagaccg
     8881 atgacggctt tttccaacca ttgtccggtg tgttcggcaa cagcatcttt attttcggaa
     8941 gtattgatat tcattattgt catgtaaatg tgtttgcaga ttgcacgtgc gggaaaatcg
     9001 ggaagggcac tattccttca gcaggtggtt gagcggcagg gaggtggtgt gtttgatttc
     9061 ttttaaaaca aagctcgatt gcgcatcttg tacgccgtgg tgggacagga gcgtatccaa
     9121 aacaaaatgg gaaaacgcgt tcatatcggt aaaaaacgcc tgaagcaggt agtcggtttc
     9181 ccctgtcagg gcgaagcagc tcaagacttc cggccatttt cggaccgatg cggcaaagtc
     9241 ttcccgcgcg tcttttgctt tgcggatgga aacgcggata aatgcctgaa gccccaggtt
     9301 gacggattcc ggagacagca gcgcggcata ttggcggacg atgccggcat cttccaactg
     9361 cttcagacgg cgcaggcacg gagaaggcga aagtgcgaca cgttcggaca gttcgacatt
     9421 ggtcagcctg ccgttttcct ggagaacctg taagatttta atatcggttt tgtctaaagt
     9481 gagttggggc atatttgcgt tccgttttaa ggaattcgga ttgtctgtcc gtatgtttgc
     9541 ggcaatccgc acagatggag accatattaa catataaaaa gttataccgt catccgggac
     9601 aaattttgtt ttcggaaaat catgtgaaaa cagaggcggt cggtttgcat ctctttaaga
     9661 cggcttgccc aaaccgccga ttcaagacat aatcgggaaa tgtgcaggag agtgttacac
     9721 ccaactacaa tgtaaccacc gaaggcgcag acacccttaa atcgctcagg tatcagggac
     9781 tgcacattga aacaaacaat ctggagagcg gcgttggaat aacgtccacc gaaggggaga
     9841 aggccgtctg aaccaccatt cagacaaccg cgcaaagcag tgagcagact ggtttgccat
     9901 catgcggata cagccgaaaa tctcaggttc aaggacagat agggtcatcc gcgcacaggt
     9961 gcgcgggcgg catctgaaca aaaaatccgg agaaacttga gaatgactgc tctgaaaacc
    10021 accccatttc atcaagccca tcaagatgca ggcgcgaagc tggtcgattt tgccggctgg
    10081 gagctgccca tccattatgg ttcacaaatc gccgaacacg aagccgtgcg caccgacgcc
    10141 ggtatgtttg acgtatccca tatgctcgtt accgacgtag caggcgcaaa tgccaaagcc
    10201 tttttccgca aattgattgc caacgatgtc gccaagctcg cttttgtcgg caaagccctt
    10261 tattccgctt tgctcaacga caacggcggt gtgattgacg acttaatcgt ttaccgcacc
    10321 aatgaagccg aaacccaata ccgcatcgtg tccaacggcg cgacccgcga aaaagacacg
    10381 gcgcaattcc acaaagtcgg acaagagttc ggcgtcgcct tcaatccgcg ctacgacctc
    10441 ggcatgctcg ccgtacaagg ccctaaagcc attgaaaaac tcctgaccgt caaacccgaa
    10501 tgggcagatg tcgtccataa cctcaaaccg ttccaaggcg cggatttggg caacgactgg
    10561 tttgtcgccc gcaccggcta caccggcgaa gacggcgtcg aagtcatcct gcccggcacc
    10621 gaagccgtcg cattcttcaa agccctgcaa caagccggcg tacagccctg cggcctcggc
    10681 gcgcgcgaca ccctgcgcat ggaagccggc atgaacctct acggcaacga tatggacgac
    10741 gacaccagcc cgctcgaagc aggtatgggt tggaccgttg acttgaaaga cgaaagccgc
    10801 gacttcgtcg gcaaagccgc cttgctggca ttgaaagaaa aaggcgttgc cgtcaaacag
    10861 gtcggcctgt tgctcgaaaa aggcggcatc ctgcgcgcgc atatggaagt gttgaccgac
    10921 aaaggccaag gcgaaaccac cagcggcgta ttctccccaa gcctgaaaca atccatcgcc
    10981 atcgcgcgcg taccgaaaga ttttgacggc gataccgcca aagtgctgat gcgtggcaaa
    11041 gaagtggacg tgcgtgtact gaagctgccg tttgtccgca acggacagaa acagtttgat
    11101 tgatgcggtt tcagacggca ttttcatttc atatgccgtc tgaaagcagg ttttaattgt
    11161 tgtccgatac ggacgtttgt agaaagcatt gaacaaggca tctgtggata ttgattcatg
    11221 cagatgccgt ctgaaaataa cccctatcaa tggagtatca aaccatgagc aacaacatcc
    11281 cggccgaact gaaatacgtt gccagccatg aatggctgcg ccttgaagaa gacggtacga
    11341 ttaccgtcgg cattacccac cacgcgcaag agctgttggg cgacatcgtg ttcgtcgagc
    11401 tgcccgaagt cggcgcgaac cttgccgctg aagagcaagc cggtgtggtt gagtctgtaa
    11461 aagccgcgtc cgacgtgtac gcaccgattg caggcgaagt cgttgccgtc aacgaagatt
    11521 tgccaagcgc tccggaaact gccaacagcg atccttacgg tgcaggctgg ttcttcaaac
    11581 tcaaaccggc aaacgttgcc gattacgaca gtctgctgac tgccgaacaa tacgcgggcg
    11641 aagtggatta aaccgcccgg ctgcccgacg gcaaccgccg gacaaacgga aactgcacct
    11701 tcagacggca tttttgcggt cggaggtgca gttttttgtc cgtgttttaa ggaagcagtt
    11761 aggctataat aacggtctat attcatcttt accgattttt tcatgcaact taccgctgtc
    11821 ggactcaatc atcaaaccgc acctttaagc atacgggaaa agctggcgtt tgccgccgcc
    11881 gccctgccta aagccgtccg caatcttgcc cgaagcaatg cggcaacgga ggcggtaatc
    11941 ctttctacct gcaaccgcac cgagctttac tgcgtcggtg attcggaaga aatcatccga
    12001 tggcttgccg attaccacag tttgccgatt gaagaaatcc gtccgtatct gtacgcgctg
    12061 gatatgcagg agactgtgcg ccatgctttc cgcgtcgcct gcgggctgga ttcgatggtg
    12121 ttgggcgagc cgcagatttt aggacagatt aaggatgccg ttagggttgc tcaagagcag
    12181 gaaagtatgg gtaagaaact caatgccctg ttccaaaaaa ccttttccgt tgctaaagag
    12241 gtccgtaccg atactgccgt cggcgaaaac tcggtttcca tggcttccgc ttccgtcaaa
    12301 ttggcggaac agatttttcc cgacatcggc gatttgaatg tcttgtttat cggcgcaggc
    12361 gaaatgattg agctggttgc cacttatttt gccgccaaaa gtccccggct gatgacggtt
    12421 gccaaccgga cgctggcgcg tgcacaggag ttgtgcgaca agctcggtgt caacgccgaa
    12481 ccgtgcctgc tgtccgatct gcctgccatt ctgcacgatt acgacgtagt ggtttcttca
    12541 acggcaagcc agttgcccat tgtcggcaaa ggcatggtgg agcgtgcatt gaaacaaagg
    12601 cagagtatgc cgttgttcat gcttgatttg gcagtgccgc gtgacattga agcggaagtc
    12661 ggcgatttga atgatgccta tctttatacg gtggacgata tggtcaatat cgtccaaagc
    12721 ggcaaggagg caaggcagaa ggccgccgcc gccgccgaaa cgctggtgtc cgagaaagtt
    12781 gccgaatttg tcaggcagca gcagggcagg cagagtgtcc ccttgattaa ggcgttgcgg
    12841 gacgagggcg agaaagcgcg caaacaggtg ttggaaaatg ccatgaaaca gcttgccaaa
    12901 ggcgcaacgg cagaagaggt tttggaacgg ctgtccgtcc aactgaccaa caagctgctg
    12961 cattcgccga cccaaacctt gaataaggcg ggggaagaag ataaagattt ggttcatgcc
    13021 gtcgcgcaga tttatcattt ggacaaataa cggtgcgccg ggaaaaatgc cgtctgaaga
    13081 ggtttcagac ggcatatttt tgtgctgtct gacaacatcg tgaaatccca cattatatcg
    13141 atgtaatcac aaagtatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac
    13201 agtacagata gtacggcaag gcgaggcaac gctgtactgg tttaaattta atccactata
    13261 ttatcccgta tgcggattgg ttttaagatt tgtaaatttg atttgcatca aaaaatcgcc
    13321 gatagatgat tcatataata tcaatattaa agagtatcgg tatatcgggg atagtcatgt
    13381 cctgtttttc aatcaaacgt atgtccgcgt ttcgggcgcg gataacggcg ttttttgccg
    13441 cctttgtctt tttgacggcg gcactgcccg cttatgcgga gcgtctgcct gattttctgg
    13501 cgaaaataca gccttcggaa atttttccgg gtgcggaccg ttacagcaag ccggaaggta
    13561 agcctatggt tgcccgcgtt tacaaaggcg atgagcagtt gggcttggtc tatatcacga
    13621 ccgatgcggt caatacgcgc ggttattcga gcaaaccgat cgatacgctg atggctttgg
    13681 caaatgacgg cacgatagcc ggagcgaaac tggtcgatca tcacgaaccg attatgctga
    13741 tcggtatccc gcatttgccc gcgcccgggc gggcgatacg cttaaactgg cttccggcgt
    13801 atatcaaacc aaactttata tcgacaaacc gattacgatt gaagggcctg ccgaccgttc
    13861 cgcaaccatc gaaggagaca ggagcgggcg taccatagcc gtacacgcgc cggacgtaac
    13921 gctccgcaac ctgaccgtta cccgttccgg tatgagcctg cctgcaatgg atgccggtat
    13981 ttatctcgaa gaaaccgccc cgcgcgccct gatcgaacac aacaatattt tggataattc
    14041 ggtcggcgta tatctgcatg gttctgccga tgcgatggtg cgggagaata aaatcgtcgg
    14101 cgacgcgact ttgcgcgtga acgagcgcgg caatggcgtt accgtttgga acgcgcccgg
    14161 cgcgcaggtc gtcggcaacg atatttccaa agggcgggac ggcatttttt ccaataccag
    14221 cacgcacaac acctataaaa acaaccgctt cagcgatttg cgtttcgccg tccactatat
    14281 gtacaccaac gacagcgaaa tcagcggcaa tatttccgtg ggcaacaata tgggctatgt
    14341 gctgatgttt tccgagcggc tcaaagtgtt tgacaatatc gccgtcggca gccgcgacca
    14401 aggcatcatg ctcaactatg tcaactattc cgatattcac gacaacatta tcaacaaagc
    14461 gggcaagtgc gtttttgcct acaatgccaa ctacgataaa ctgtccgcca atcattttga
    14521 aaactgccaa atcggcatac actttaccgc cgccatcgaa ggcacgtcac tgcacgacaa
    14581 ttcctttatc aacaacgaaa gccaggttaa atacgtcagc acgcgctttc tcgactggag
    14641 cgagggcgga cacggcaact attggagcga caacagcgcg ttcgatttga acggcgacgg
    14701 cttcggcgac agcgcgtacc ggcccaacgg catcatcgac caaatcatct ggcgcgcgcc
    14761 cgtatcgcgt ctcttgatga acagtcccgc aatcagcatc gtcaaatggg cgcaggcgca
    14821 gtttcccgcc gttctgcctg gcggcgtggt ggacagcaaa ccgctgatga agccttatgc
    14881 ccccaaaatt caaacccgtt atcaggcgat gaaggacgag ctactcaaag aagtcgaaac
    14941 gcggcagtcg gaatggggca gggcggaaaa cggttctttg aactagtctg cttcagacgg
    15001 catccggatt caaatgccgt ctgaaaacac aaaaggaaca accatgacca cacatcatgt
    15061 cgaattgagg aaggtaacca aacggttcgg ggcgcaaaaa gccgtcaacc aagtcgattt
    15121 ggttttgaag gcaggagaaa gcgtcgggct tgccggacac aacggcgcgg gcaagtccac
    15181 cattatgaag ctgatactcg ggctgattac cccgaccgaa ggcgaagtga tgcttttggg
    15241 cgaacgtacc ggtagcaaag cgggggcgcg gcttcgcagc caaatcggct acctgcccga
    15301 aaccgttgcg ctgcaccctt cgctgatcgg catcgaaacg ctggattttt atgccaaact
    15361 taaaaaacag ccgctcacgc agaaccgggg gctgcttgag cgcgtcggca tttcacaggc
    15421 ggcacaccgc cgcgtcggca cttattctaa agggatgcgc caacgccttg ccttggcaca
    15481 agccctgctg ggcgagccca aagtcctgct gtttgatgaa ccgacaaccg gtcttgaccc
    15541 tgcatcacga caaatgtttt acgaagtcgt gcgcgaactc aacgggcgcg gcgcgaccgt
    15601 attgctcagc acccacgccc ttgccgagtt ggacgggcac gccgaccgca ttatcgtgga
    15661 ttaaatttaa tccactatat gcgggtatgg cgggtttgag cggacaaatc agcctgaccg
    15721 tccccgtttt gctgaccgct caggttttat gggttatcat tccgcttgtt ttggcagccg
    15781 gaatttttag aaagcgacaa atatgaaaaa aaccctgttg gcaattgttg ccgtttccgc
    15841 cttaagtgcc tgccggcagg cggaagaggg accgccgcct ttaccccggc agattagcga
    15901 ccgttcggtc ggacactatt gcagtatgaa cctgaccgaa cacaacggcc ccaaagccca
    15961 gattttcttg aacggcaaac ccgatcagcc cgtttggttc tccaccatca agcagatgtt
    16021 cggctatacc aagctgcccg aagagcctaa aggcatccgc gtgatttacg ttaccgatat
    16081 gggcaatgtt accgattgga cgaatcccaa tgccgacacg gagtggatgg atgcgaaaaa
    16141 agccttttac gtcatcgaca gcggctttat cggcggtatg ggtgcggaag acgcgctgcc
    16201 gttcggcaac aaagagcagg ctgagaaatt tgcaaaggat aaaggcggta aggttgtcgg
    16261 tttcgacgat atgcctgata cctatatttt caaataatat tgaaaaaacc ggccgaaact
    16321 ttaaaaacga gtttcggtcg gtttttcttt tcttgttcgg tatagtgtcg gcaggaaaga
    16381 accttcacat cccgccgtaa ttcggcccgc tcgcgccttc ggggcaaatc caagtgatgt
    16441 tttgcgtcgg gtctttgatg tcgcaggttt tgcagtgcac gcagtttgcc gcgttgattt
    16501 gcaggcgcgg attgccgttt tcttcaacaa tttcgtacac gccggccgga caatagcgcg
    16561 tttcgggcga ggcgtattct ttgtagttca cgtctatcat cgtttgcgga ttgttcagca
    16621 ccaaatggtc gggctggttt tcttcgtgcg cgagattggc aaggaagacg ctgctcaagc
    16681 ggtcgaaggt caacacgccg tcgggtttcg gataatcaat cggcttacac gcggcggctt
    16741 ttttaagctg ctcgttgtct ttgccgtgat gtttcaaggt ccacggggct ttgcctctga
    16801 aaatcatctg atcgatgccg gtgtagattg agccgaggta aacgccccat ttgaatgacg
    16861 gacggacatt gcgcgcggcg taaagctctt gatacagcca gctttgttca aaacgttgct
    16921 gataatccgc cgcctctttg ccgctgtcga aaccctccac ttcttcaagg ttttccaaca
    16981 aggggaacac ggcttcggcg gcgagcatgg cggatttcat cgcggtatga atgcctttga
    17041 tgcgcggcat attgaggaaa cccgccgcat cgccgaccaa aatgccgcct ttgaacgaga
    17101 gcttcggcaa actttgcaaa ccgccttcaa tcagcgaacg cgcgccgtaa gcaatgcggc
    17161 ggccgccttc aaaggttttg cggatttcgg gatgggtttt gaaacgttgg aactcttcaa
    17221 acggcgacag ataaggattt tgatagtcca aaccgaccac gaagccgacg gcgactttgt
    17281 tgtcgtcgaa atggtaaaca aacgcgccgc cgtaggtttt gctgtccagc ggccagcctg
    17341 cgctgtgcac caccaaaccg ggctgatgct gttcggacgg cacttcccaa acttctttaa
    17401 tgcccaagcc gtaagtttgc ggctggctgt tttggtcgag ttggaaacgt tcgatgattt
    17461 gtttggaaag cgaaccgcga caaccttcgg caaacagggt ttgctgcgcc caaagctcca
    17521 tgccgggttg gaatgaatcg gtcggctcgc cgtctttgcc aatgcccata ttgccggttg
    17581 caatgccttt gaccgaaccg tcttcgtgat acagcacttc ggcggcggca aagcccggat
    17641 agatttccac gcccatattt tccgcctgct ccgccaacca gcgcacgact tcgcccaagc
    17701 tgacgatgta gttgccgtga ttgtcgaaat tcggggtaat cggcaggttg aacgcttttt
    17761 tctcggtcag gaacaacact ttgtcctgcg ttactgtgcg tgtcagcggt gcgccttttt
    17821 ctttccagtc ggaaatcaac tcattcagcg caatcggatc gataactgcg ccagccagcg
    17881 aatgcgcccc cacctccgaa cctttctcca ccacgcaaac gctgatttcg cgcccgtttt
    17941 gttcggcaag ctgcttgagt ttgatggcgg cagacaaacc cgacgggcct gcgccgacaa
    18001 tcacgacatc gtattgcata ctgtcgcggg tgatggattc tgtcatggcg gttcctgtgt
    18061 atttattatt gaattgcaaa tccgtaatta tacaacggga acatatagtt accaaataca
    18121 acaaaggtcg tctgaaaacc atattttcgg ttttcagacg acctttgtcg aaatttcaat
    18181 aagcacgcca ccattttacc tgtccgaccg caaactccgt ctgacgtttc ggactgcgtg
    18241 tgaaaaacgc cttatccccg ccggcatccc tccctttcgg cacaaccgcc aaaatcttac
    18301 ctgccaaatt tccctcacgg gtttgccaag catccaaaaa ctgcgccctg ctcatcgaaa
    18361 catgacccag cgacgggtcg gcaagcaaaa ccgtattgcc gtctataccg cgcaataccg
    18421 agaaatgatc atccttgcgg tatttcagat acacgatgac ggggatttgc aactgtgcaa
    18481 gctgctcgaa agacagggca tagcctttcg cttcaaaacc caaatcaggc ataatgcgcc
    18541 gcatatcctc aaacgacgcg ggcatctgct ccttatccag tttttttaac acgtcctctt
    18601 ccgtcagctt ttgcccgtaa aaattgttca aaagcgtcgc caccgaagcc gccccgcagg
    18661 aaaaatccaa atcctgcttt acaatattga aatcgcgc
//
LOCUS       DAWHRA010000029        18236 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWHRA010000029 DAWHRA010000000
VERSION     DAWHRA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18236
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.029"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(149..1081)
                     /gene="cysK"
                     /locus_tag="WIB30_000728"
     CDS             complement(149..1081)
                     /gene="cysK"
                     /locus_tag="WIB30_000728"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ3796826.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1275..1880)
                     /locus_tag="WIB30_000729"
     CDS             complement(1275..1880)
                     /locus_tag="WIB30_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796827.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="WIB30_000730"
     CDS             complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="WIB30_000730"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ3796828.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAEWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            2865..3161
                     /locus_tag="WIB30_000731"
     CDS             2865..3161
                     /locus_tag="WIB30_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796829.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3175..3843
                     /locus_tag="WIB30_000732"
     CDS             3175..3843
                     /locus_tag="WIB30_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ3796830.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3916..6039)
                     /gene="pnp"
                     /locus_tag="WIB30_000733"
     CDS             complement(3916..6039)
                     /gene="pnp"
                     /locus_tag="WIB30_000733"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ3796831.1"
                     /translation="MMFDKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVA
                     VTTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNG
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWS
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTS
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKI
                     REVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6273..7136)
                     /locus_tag="WIB30_000734"
     CDS             complement(6273..7136)
                     /locus_tag="WIB30_000734"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ3796832.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDENGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7336..8199
                     /gene="rfbD"
                     /locus_tag="WIB30_000735"
     CDS             7336..8199
                     /gene="rfbD"
                     /locus_tag="WIB30_000735"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ3796833.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALSAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FVQHIFQAASQQQTSFPIPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(8674..8907)
                     /locus_tag="WIB30_000736"
     CDS             complement(8674..8907)
                     /locus_tag="WIB30_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ3796834.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(9073..9273)
                     /locus_tag="WIB30_000737"
     CDS             complement(9073..9273)
                     /locus_tag="WIB30_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ3796835.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(9445..10320)
                     /gene="xerD"
                     /locus_tag="WIB30_000738"
     CDS             complement(9445..10320)
                     /gene="xerD"
                     /locus_tag="WIB30_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ3796836.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(10372..10812)
                     /locus_tag="WIB30_000739"
     CDS             complement(10372..10812)
                     /locus_tag="WIB30_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ3796837.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            11236..12174
                     /locus_tag="WIB30_000740"
     CDS             11236..12174
                     /locus_tag="WIB30_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ3796838.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYSSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            12302..12595
                     /gene="hfq"
                     /locus_tag="WIB30_000741"
     CDS             12302..12595
                     /gene="hfq"
                     /locus_tag="WIB30_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ3796839.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            12680..13246
                     /locus_tag="WIB30_000742"
     CDS             12680..13246
                     /locus_tag="WIB30_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3796840.1"
                     /translation="MLLKNILPFAHCLLRQALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            13300..13674
                     /locus_tag="WIB30_000743"
     CDS             13300..13674
                     /locus_tag="WIB30_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ3796841.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            13684..14178
                     /gene="folK"
                     /locus_tag="WIB30_000744"
     CDS             13684..14178
                     /gene="folK"
                     /locus_tag="WIB30_000744"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ3796842.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPHLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVTELSKQLGNQGIRL
                     LPEE"
     gene            complement(14314..14787)
                     /locus_tag="WIB30_000745"
     CDS             complement(14314..14787)
                     /locus_tag="WIB30_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ3796843.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSAYKRALSALSRKL
                     GRCKDKLHTAVQPVLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAML"
     gene            complement(15007..15744)
                     /gene="ubiE"
                     /locus_tag="WIB30_000746"
     CDS             complement(15007..15744)
                     /gene="ubiE"
                     /locus_tag="WIB30_000746"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ3796844.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKSGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(15787..16155)
                     /locus_tag="WIB30_000747"
     CDS             complement(15787..16155)
                     /locus_tag="WIB30_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ3796845.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQLVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            16275..17894
                     /gene="mnmC"
                     /locus_tag="WIB30_000748"
     CDS             16275..17894
                     /gene="mnmC"
                     /locus_tag="WIB30_000748"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ3796846.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPTFVRALLSHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGAHSSC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAQLSPALAESLFAANPNPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(17891..18040)
                     /locus_tag="WIB30_000749"
     CDS             complement(17891..18040)
                     /locus_tag="WIB30_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796847.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT         4670 a         5410 c         3910 g         4246 t
ORIGIN      
        1 atatttccct caagcaatgc ggcggcacca atacctgatt aaaacacaac cggtgttttc
       61 ggcatatatg caaaaacgca ttttccggca cggcgttaca ccgataccga aaaatgcgtc
      121 tgaatgtttc gacaatccga ttaaagcatt atgccaaatc tgcaaaaagt ggcgtagaga
      181 gatagcgttc gccataagaa ggcagcagca cgactatcag cttgccttcg ttttcaggct
      241 gtttggcaag ctgcaacgca ctccaaaccg ccgcaccgga agaaataccc accaaaatgc
      301 cttctttttc cgctattgcg cgggcggttt caaaagccgc ttcgttcgac actttggtaa
      361 tgctgtcgta aattttggta ttcaaaacgg tcggaataaa gcctgcgccg atgccttgaa
      421 tcgggtgcgg gcctttttcg ccgccgctca atacgggtga agcttcaggc tcgacggcaa
      481 ccactttaac ttcgggtttg tattttttca acacttcgcc cacgccggta atcgtaccgc
      541 ccgtgccgac gccggcaacg aagacatcga ctttgccgtc cgtatcccgc caaatttcct
      601 cggcggttgt tttgcggtgg acttcggggt ttgcctcatt gtcgaactgg cgcggcataa
      661 aataagtgtc gggatgcgcg tccaccaagg atttcgcttt ggcaatcgcg cccgccatac
      721 cttcggcggc aggggtcaga atcagctccg caccaaacgc gcgcaacagc attttgcgct
      781 ccttactcat gctttccggc atggtaatcg ccagcttgta gccgcgtgcg gcacatacca
      841 ttgccaaacc gacacccgta ttgccgctgg ttgcttcgac aatgacggtg tttttgttga
      901 ttttgcccgc tttttcggca ccctcgatca ttgcttcggc aatgcggtct ttgacgctgc
      961 tgcctggatt gaaaaattcc agtttcacgg caacctccgc cttcaaacct tcggtcagac
     1021 ggttcagttt gaccaaaggc gtgttgccga ttagttcggt gatgctgttt gcaattttca
     1081 tcttctgctc ctgaataata aggtttcgac agtaaggcgc aatccccata ggcatcctgt
     1141 ccgattgcag gggatttgcg ccgaaacata aacaaatttc ggatatggct ttatcctaaa
     1201 cctatgccgc ctaaaaaacc aatatccaaa atttctttct ttataacttt ttgttccgat
     1261 acggccgcac cgtattatct tccaatttct gaaagcagca gcaccagtac cgagacctgc
     1321 atcaacgctg ccgtcaaccc gccgagtcgc agctgcgagc cgccgaacgt tgacggcggc
     1381 tgccggtcta taccgcagcg gtggacatag gcaaccgcca tcagcacaaa ctgcaacacg
     1441 aaccaataaa ccggcttcag cgcataaagt ttgagcagtg ccgcaaacac aaacacttga
     1501 accgttcccg aaaagcaata atgcaccaac gcacatattc cgacaaaagc aagcgtcatc
     1561 gatacattac tgacggcaaa aagcccgagc agcggatgtt cggggtctgc ctgccatccg
     1621 tttccatctg ttttccggtt ccaatgcccg atgaaaaaaa atatgctgcc caaagtcagg
     1681 taaaaatggg ggatgaacaa gggcgcggcg cgggtcattc cccatatgcc gggcatcagc
     1741 tttgccccca aaaccgatat gcccagccac agcataatac tcgcccaaaa ccattgcaac
     1801 attcctgcgc ggaatatcag aaacagaaaa accgccgcat acagtaaggc agacaaaaaa
     1861 tccaaatgca gcaaaatcat gaatacgcca actcaccttc aaacaccgct tccgcagggc
     1921 cggtcatcat cacatcgccg ccgcaggccc attcgatata taaagtcccg cccggcaaaa
     1981 ccacctctac cgttttccct tcatccaaca gccccagacg gatacccgcc accacagccg
     2041 cacacgcgcc cgtaccgcaa gcttgggttt cgcccacgcc gcgctcgaac acgcgcaaac
     2101 gaatcgcggt tcggccgaca acctgcataa agccgacatt gacgcgttcg ggaaactgcc
     2161 tgtgcggttc gataagcgaa ccggtttcgc gcaccggcgc gcattccacg tcatcgacca
     2221 caatcaccgc atgggggttg cccatattga cgcagctgac aggctgaatg ccggattcga
     2281 gatgcacccc gtaaatacag gcatcatccc cctcgcccga ttcggggaca aacggtattt
     2341 cagacggcat aaacttcggt ttgcccatat tgaccgtaac cataccgtta tcggacaatt
     2401 tcggaaaaat aacgccattt gccgtttcaa cacaaatttc tttcttatcg gtcaaaccct
     2461 tgtctgcaac aaaacgggca aaacaacgcg ctccgttgcc gcattgcccg acctcgctgc
     2521 cgtcggcatt gaaaatacgg taacggaaat ccacgccttc ggtttccgaa cgcccgacca
     2581 ccaaaagctg gtcgaagccc acgccccgga agcggtccgc ccattccgca atcggcgcgt
     2641 cctcgggggt aaaatcctga ctgaccgcgt caatcaccat aaaatcgttg cccaaaccgt
     2701 gcattttggt aaatttcaaa gtgttcatat tgttccgaaa tcccgtatcg tctgttgctt
     2761 ccgaatccga aatcccccgc accgaaggga aatgcttcca tctcccgccg cctgaggatt
     2821 aaaatcagcc attatactgt ttgttacccc ttacaagaaa caccatgttc cgcatattac
     2881 tgttgctttt cccttgtgca atccttgccg cctgcacccg aaacataccg gcaaacccgg
     2941 ccgtttcaaa ttgcgcgcat cttcaaaacc gcaccatcct gtattttgac gcacacggca
     3001 aacccgccga cggcgcagta aagggcggat tttacaaaat tatcgaaata cagggtgcaa
     3061 actgcatcct tattcaagac tttaacagca cgggcaatgc cctcggcgga tcctatgtgc
     3121 cggactgccg caaatcatcc cttttcccga aaaatatttg acttcaaact gcctatgaac
     3181 cacgacatca ctttcctcac cctgttccta ctcggtttct tcggcggaac gcactgcatc
     3241 ggtatgtgcg gcggattaag cagcgcgttt gcgctccaac tccccccgca tatcaaccgc
     3301 ttttggctga tcctgctgct taacacagga cgggtaagca gctatacggc aatcggcctg
     3361 atactcggat taatcggaca ggtcggcgtt tcactcgacc aaacccgcgt cctgcagaat
     3421 attttataca cggccgccaa cctcctgctg ctctttttag gcttatactt gagcggtatt
     3481 tcttccttgg cggcaaaaat cgagaaaatc ggcaaaccga tatggcggaa cctgaacccg
     3541 atactcaacc ggctgttacc cataaaatcc atacccgcct gccttgcggt cggaatatta
     3601 tggggctggc tgccgtgcgg actggtttac agcgcgtcgc tttacgcgct gggaagcggt
     3661 agtgcggcaa cgggcgggtt atatatgctt gcctttgcac tgggtacgct gcccaatctt
     3721 ttagcaatcg gcattttttc cctgcaactg aaaaaaatca tgcaaaaccg atatatccgc
     3781 ctgtgtacgg gattatccgt atcattatgg gcattatgga aacttgccgt cctgtggctg
     3841 taagccccat tcattcccga tacccgtaaa aaataccgtc tgaaacctgt tcagacggca
     3901 ctttcaccct aagcgttact cggcggcatt ttcctcacgg gcaggcgcgt ccagcagggc
     3961 tttgatggac agacggacac ggcctctgtc gtccacttcc aatgctttca cgttcaccac
     4021 ctgaccgact tgcaggtagt cgccgacatt gcgtacgcgc tcgtgggcga tttggctgat
     4081 gtgtaccaaa ccgtctttgc ccggcatcac gctgacaatc gcgccgacat tgttgtcgag
     4141 gattttcacc acagtgcctt cgtacacttt gcccacttcc acttcggcag taatctgctc
     4201 gatgcgtttt ttcgccgcat cgccggcttc ttgagtggtt gcggcaatgg taatcgtacc
     4261 gtcttcggca atattgattt ccgtaccggt ttcagcggta atcgaacgga tggtttcacc
     4321 gcccttaccg ataacttcgc ggattttgtc ttggttgatt ttcatcgtga acaagcgtgg
     4381 cgcgtgtgcg gacagctctt gcgggcccgc aacggcggct ttcatctgat ccaagatgtg
     4441 cagacgcgct tctttggcct gtgccaaagc gatttgcata atttctttgg taatgccttg
     4501 gattttgatg tccatttgca gcgcggtaac gccttcggtc gtaccggcca ctttaaagtc
     4561 catatcgccc aagtggtctt cgtcgcccaa aatgtcggtc aggacggcaa atttgttgcc
     4621 ttccagaatc agacccatcg cgataccggc aacgtgtgct ttcaaaggca cgccggcaga
     4681 cagcaggctc aggcagccgc cgcagacgga agccatagag gaagagccgt tggattcggt
     4741 aatttcggag accacgcgca tggtgtagct gaaatcttca ggtttcggca atacggccaa
     4801 caatgcacgt ttagccaaac ggccgtgacc gatttcacgg cgtttcggtg cgcccatgcg
     4861 gcccacttcg ccggtagagt acggcggaaa gttgtagtgc agcataaagc ggtcggtgta
     4921 ttcgccggac agcgcgtcga tgatttgctc gtcgcgcgaa gtacccaaag ttgcaacggc
     4981 caaagcttgg gtttcgccac gggtaaacaa tgcagaaccg tgcgtgcgcg gcaatacgct
     5041 ggtttggatg ttcagcggac ggacggtgcg ggtgtcgcgg ccgtcgatgc gcggttggcc
     5101 atccaaaatt tggctgcgga cgacatcggc ttccaagtgt ttgaaaatgc ctttgatttc
     5161 gttggctgcc aaagtgtcgg tttcttcggt aatcaaggct tcttttaccg cactccaagc
     5221 ttcgtccaat ttggcagaac gcgcttgttt ttgacggatt ttgaacgctt ctttaatggt
     5281 ttcgccggca atcccgcgga ctttggcaac cagttcctca ttggtttcag gtgctttcca
     5341 atcccaaagt tccggattga cttcgtcggc aaattcattg attgcattga tggcaacctg
     5401 catttgatcg tggccgtaaa ccaccgcgcc cagcatcacg tcttcgggca ggattttggc
     5461 ttcggattcc accatcaaca cggcttttga agtaccggcg accaccaagt ccaattgcga
     5521 tttcgccaat tcggctttag tcggattcaa aacgtacacg ccgtttacat aaccgacgcg
     5581 tgccgcgccg atcgggccgg caaacggtac gccgctcaac accagcgcgg cagatgcacc
     5641 caacattgca ggaatatcag aatcgatttc aggatcgacg gacacgacca tcgctacgat
     5701 ttggatgtcg tggtagaaac cttcagggaa cagcggacga atcggacggt cgatcagacg
     5761 gctggtcagg atttcttttt cgctttgttt gccttcgcgt ttgaagaaac cgccgggaat
     5821 tttgcctgcg gcgtaagtgc gttccaaata atcgacggtc agggggaaga agtcttgacc
     5881 ttctttcact tctttgttgg tggtaacggc aaccaaaaca acggtgtcgc ccatagagac
     5941 tttaacggca gcggcggctt ggcgggcaat ttcgccggtt tccaaagtaa cggtctgatt
     6001 accgtattgg aaggtcttaa cgtgtttgtc gaacatcatt gttcctttca aaataccgca
     6061 ctgctaaaac actaataatg cacactaaaa tccgaatgtg catagttagg gtttcagacc
     6121 gtgcggcagg ttataaacaa gctttcagac ggcatttcgg acgctgaaag caattgcgga
     6181 ttataaaggc aaccatcctt aaaatccagt attgatacaa taaaaaggcc gtctgaacca
     6241 tatttctttc agacggcctt caaacttaaa aatcaatcct gagtcagcaa agtcaatgct
     6301 tcccgatact tctcgacagt tttctgaatc acatcggcag gcactttcgg cgcaggggct
     6361 tttttgttcc aaccgctttg ttccagccag tcgcggacga attgtttgtc aaaagacggc
     6421 ggattggtgc cgactttgta ttggtcggca ggccaaaaac ggctcgaatc gggagtcaat
     6481 acctcatcca tcagcgtcag cgtaccgttt tcatccaaac cgaattcaaa tttggtatcg
     6541 caaataataa taccgcgcga tttggcatat tccgccgctt cggtgtaaag ccgaaccgcc
     6601 ttggcgcgca cttcttccgc caattctttg ccgataatgc gtccgcattc ttcaaagctg
     6661 atgttttcat cgtgatcgcc gactgcggct ttggttgagg gcgtaaaaat cacttcaggc
     6721 agttgttgcg cttcctgcat accttcaggc agttgaatac cgcaaaccga gccggttttt
     6781 tgataatctt tccaaccgct gcctgccaga taaccacgca caatcgcctc tactttcacc
     6841 ggagtgagct ttttagccac gacggcgcgt ttctctaaag ctttggcttc gttttcaggc
     6901 aaaacatcgt aaaccgtttg accggtaaag tggttgggca taatatgcgc cagtttttta
     6961 aaccaaaaat tggaaatctg cgtcagaatc tcccctttgc tcggaatcgg gtcgtccaaa
     7021 atcacatcaa acgcggacag gcggtcggaa gcgaccatca gcatacgttt atcgtcgatt
     7081 tcatataaat cgcgcacttt tccagaatag atctttacca aaccaatctc actcatttcg
     7141 cacccccctg aaaatatctt gaaaataccg acccgacacc cgacaggttt gaatcacaaa
     7201 ccgatattct aaccgaagtc ggcgcaaaac aatacccatg gcacaaaaag ccaacccgtc
     7261 aaccgtcggc aaagttttgg cactataata ccgacagcaa gtcctacaat acactttcac
     7321 caaaggaaat acctcatgag aatcctattg acaggctcga aaagccaact ggcacgctgc
     7381 ctgcgcgacc gtcttccgga agactgggaa accattgcga cggattccgc atccctagac
     7441 attaccgatg ccgatgccgt ctgcaacatg gtcaaaagtt tccaacccga cgccattgtc
     7501 aacacggctg cctatactgc cgtcgacaaa gcggaaggcg atgcggcagc ggcatttgcc
     7561 gtcaatgctt ccgccgttta caaccttgcc ttgtcagcac atcgcgccca tgcccgattc
     7621 atccacatct caaccgacta tatctttgac ggtaaaggga aaagacccta tcaggaaagc
     7681 gactttacca atccttccaa tgtatacgga caatccaaaa ccgcaggcga gctgctcgca
     7741 ctgtctgcca atcccgacag ccttatcctg cggacttctt ggctgtttag cgaatacggg
     7801 gacaactttg tccgcacgat gctgaacctt gcacgggaac gttccccgct gtccgccgtc
     7861 cacaaccaaa tcggctgccc gacctatgcc ggcgatttag ctgccgccat catccgcctt
     7921 ttgcagcaaa cgtcgcccgt gcgcggcatt taccactacg ccggcggcaa atccgtatct
     7981 tggtacgaat ttgtccaaca tattttccaa gcggcatcgc aacagcagac atccttcccc
     8041 attcccgaat tgactgccgt ttcagacaag gaatatccga ccgccgcccc caggcccgca
     8101 tacagcattt tggactgccg caaaatcgaa aacgacttcg gcatcaaacc gtcagactgg
     8161 caaaaagccc ttgcgcaggt cgtttccaag ctgctctgat gccgcccgcc tctccgtttc
     8221 cgccgtcaag caccgccttg gcggttttct tatatagtgg attaacaaaa accagtacgg
     8281 cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     8341 tttgttaatc cactataaaa tctaccgatt acacaaacac atatcatctt tacacaatca
     8401 tgcttccatc aacagtaaaa catgatatga ttgccaacaa taacatctca caataaattt
     8461 tctaattttt attgaaaaaa tcaataaata agaattctcc caaccgacaa ataaaataaa
     8521 gaaagggtta atatgcaaca tcgaagaaga ctagcaattt accaagcatc caaacgtgct
     8581 tcctttaccg gcaggtcatc cgccccgcaa aaacgtaaag aacgttgatt tgaaaaaaat
     8641 gccgcctgaa gtcctgcttc agacggcatt tttttaccgt tcgagaaact gtttcaacct
     8701 gtcctcatcc aaaaaccagt gacagatttc ctcatctccc gccaacaaaa cgggaaccag
     8761 ctcattgtat ttttcttcca aaacaggatt ttcatccaca tcgaccactt ccagcccgaa
     8821 cccgtattca ttttgaaaag gtttgagtgc atcgcgcatt ttgtggcaca agctgcaata
     8881 ttcacgaaac atcaaggtca atttcatccg cgtttcctta tctgtcaatt tgcacacgcc
     8941 aaagccttag acgcagcaga atcatggtct atttgggaaa aaacaatgtt ttcaaggaag
     9001 atgatactca agttctgcca aaaacagcaa aaatgccgtc tgaacagttc agacggcatt
     9061 tcgaaaaccg ttttacgctt gaacgttgat acccgccgta accgtcggtt gcgcattgtg
     9121 cgccgtcaga aacgaagcgg caagctcccc gcagcagccg cagcagctcg ctacgcccaa
     9181 ttgcgactgc aaatcgccca ttgtggtcgc gccggcggcg atggtttcct tgatttgatg
     9241 gtcggtaacg gcattgcaga tgcagacaaa cattttgtgc tccgtgtgtt ctcaaactat
     9301 cgtaccgata acggctttta ttatcgtatg caaatataaa taaaaacggt tcgcattgca
     9361 aggtcggcat acacggttta tcttggtaat tttttatcaa gtttgtattt tcgattgatt
     9421 tcttattaaa ccgaagcaga agcctcagtt tcgggaatgg tgttccttca ctacaccctg
     9481 caaccatacg ttggcaacat gggtataaat ctgcgtcgta ttcaaatcgg catgtcccaa
     9541 catatcctga accacgcgca aatccaagcc gtgccgcacc agatgcgtgg caaaggcgtg
     9601 gcgcaggctg tgcgggctga tgtgcccgat gcctgcctga cttgcatatt ctttgacaat
     9661 catccatgcc aactgacgag aaatgcccgt ctttttctga ctgacaaaca atgcgtcgca
     9721 attcctgcct ttcagcagaa gtgggcgtgc ctccgtataa tagcgttcca cccaatacgc
     9781 cgactcctgc cccatcggga ccatcctctg cttatcaccc tttcccagcg cggtaataca
     9841 gcccctgtcc aaatccacat tgccgaagtt cagcccgacc gcctcgctga cgcgcaagcc
     9901 ggtcgcgtac atcaattcga gcagtgcctt gtcccgcaaa ccgtgtggcg tatcagtatc
     9961 cggagtggca agcagccggg aaatctgctg ttctgtaatc agtgtcggaa tattcttgtc
    10021 gattttgggc ggtttcagca aacgggtggg attgtccgtc cttatgcctt cacgctccat
    10081 ccatatatac aggcgtttgc acgccgataa tgcgcgcgcc tgcgaactcc gttgctctcc
    10141 gtcaacataa accgccgccg ccaaatccgc ttcgtccgca tccttcagca ttctgcccga
    10201 ttgggacagg cggcgggcga ttttttccaa atcgcgccgg taaccgttta aagtattctg
    10261 actgagccgc ctgtccaacc acagcgtttc aagcagcctg tcgatcaaac cttcttccat
    10321 accgttccaa acaaatgccg tctgaatctt cttcagacgg catggttcac attatcggga
    10381 aagcgtttcc aatacttcct gcgcgtgacc cgccactttg actttccgcc attcatgggc
    10441 gatttctcca tccttattca agacgaacgt actgcgctcg atacctaacg actctttccc
    10501 gtacagtttc ttcaatttga tgacatcaaa caggcggcac actgtttcat ccttgtcgct
    10561 caacagctcg aaccggaaac cctgcttggc gcaaaaattc tgatgcgcct ttacgccgtc
    10621 gcgggaaata ccgaccacgg tataacccaa tgcctcaaac tgttccaaac gcgcattgaa
    10681 atccaagcct tccgtcgtac agccaggcgt actgtctttc ggataaaaat acacgaccaa
    10741 aggcagatgt tctgccgaat gaaaatccgc accgctgctc gaaggcaggg taaattcata
    10801 tttcacatcc atagtcctac tcccgatatt cccattattc aaaacggcac gcagaggcac
    10861 cgccgcaatt tccaaaccaa ccccgattct accgccccaa aggacaagga ttcaaccgcc
    10921 ggaaacatcc aaaccgacac acgacggcat gaaaaatatc catgtcaaac cacaaaatat
    10981 agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa atagtacggc
    11041 aaggcgaggc aacgccgtac tggtttttgt taatccacta tatgttccga tttaaaaaca
    11101 gaatgttata aaacccaatc cccaaaacac aacaagaccg cccgcctacg ggcagtctcc
    11161 tgtcagacga catactttac agatggctgt tttttcaaca aaataacgcc aatactcaaa
    11221 aatatggaat caaaaatgtc catccatact ctgaaacgcc tgccctcatc gctgctgctc
    11281 ggtctctgcc tttccctgcc gtcagcccac ctttttgccg acaacgacat tttagggcaa
    11341 tttttagaac agaacatgct tacctcctcc gatccgatag aaatattcgc cgaaagcacg
    11401 atacacccca ccaacaccca agccattaca ggcggtctga ttctctcctc acagtctgcc
    11461 ctggtcgtca acaacaaaac cggacagata ctgtatcaga aaaacgccga caggattatg
    11521 cccatcgcct ccatttccaa actgatgagc gcgatggtcg ttttggatgc aaacttggac
    11581 atgaacgaaa ccgttaccat tacgcccgac gaaatcgacc gcatcaaagg gaccggcagc
    11641 cgtcttgcca taggtacggc acttacacgc aaagaactgc tgcacctgag cctgatgagc
    11701 agcgaaaacc gcgccaccca tgcattgggc agaacctacc ccggcggcat gggcgcattt
    11761 gtcgccgcta tgaaccgcaa agcccaaagc ctgggcatgt acagcagccg cttttacgaa
    11821 ccgaccggac tcaacttcca aaacgtttcc accgccaaag acctgagcct tatggtcaac
    11881 gccgccgccc aatatccgca aatccgcacc aactcgactt ccaactacgc ctcggtacag
    11941 accaaaaacg ggcagcagaa ctacaaaaac tccaatgcgc tggtcaggga aggcatgtgg
    12001 aacatcgaat tgcagaaaac cggctacata cgcgaagcag gcaggtctat ggttgtcaaa
    12061 gccaacattc aaaaccaacc cgttaccatc gtattgctga actctcccac atccgccaca
    12121 cgcgtcaacg acgcccgcaa aatcgaatca tggatgctgc agcaacgctc ctgacataca
    12181 aatgcccggc ggaaaaccgc ccaaacacaa aaaaattggt aatttttcct attcaaggct
    12241 acaattcgac acgcacaggg catctgcccg atttcacaat cataatagcg gagttaaaaa
    12301 tatgacagct aaaggacaaa tgttgcaaga tcccttcctg aacgccctgc gtaaagagca
    12361 tgttccggtt tcgatttacc tggtcaacgg catcaaactg caaggtcagg ttgaatcttt
    12421 cgaccaatac gtcgttcttc tgagaaacac ttccgtcacc caaatggttt acaaacacgc
    12481 catttccact atcgtaccgg ctcgctccgt caacctccaa catgaaaaca aaccccaagc
    12541 cgcaccggct tcgacccttg tccaagtgga aaccgtccag cagcctgccg aataatccgc
    12601 acgaagcatg acgtgtcata tctttcaata ccttaccgga caacggtaag gtatttttat
    12661 tttcagacgg catttcaaaa tgttattgaa aaacatcctt ccattcgccc attgcctttt
    12721 gcggcaggca cttcccgaag gcggcaatgc tttggacggc accgccggca acggacacga
    12781 cacccttttc ctcgcacaaa ccgcaggcat ccgggggaaa gtgtgggcat tcgacatcca
    12841 gccgcaagcc ctgagccgca ctcgatgccg tctgaaagaa gcgagatacg agaatgtgca
    12901 actcatctta gacggacacg aaaacctgaa acaatatgtt tcagaaccac taaatgccgc
    12961 cattttcaac tttggctggc tgcccggcgg agataaaagc cttaccaccc atacggaaac
    13021 cagcattccc gccctttgcg caaccctctc cctgctgaaa gaaggcggta tgcttattgc
    13081 cgtcctctat ccgggacacg aaaacggcaa acaggaggca gaagcaatcg aacaatgggc
    13141 aaaaaacctg cctcaagaac agtttgccgt tttgcgttac ggctttacca accggaaaaa
    13201 cagcccgccc tatctgttgg catttgaaaa gttacgtcaa aaataaatgt ttgcggtaaa
    13261 ataagccgat tccaaccctt attttgataa gagccgacca tgcagtatct gattgtcaaa
    13321 tacagccacc aaatcttcgt taccatcacc attttggtat tcaacatccg tgttttccta
    13381 ctttggaaaa atccagaaaa gcccttggtc ggcttttgga aggcactgcc ccaccttaac
    13441 gacaccatgc tgctgtttac aggattgtgg ctgatgaaaa ttacccattt ctccccgttc
    13501 aacgcgcctt ggctcggcac aaaaatcctg cttctgctcg cctatatcgc attgggtatg
    13561 atgatgatgc gcgcccgtcc gcgttcaacc aagttctaca ccgtttacct gctcgctatg
    13621 tgttgcgtcg cctgtatcgt ttaccttgcc aaaaccaaag tcctgccatt ctgaaacact
    13681 gccatgaaca acagacattt tgccgtcatc gccctgggca gtaatcttga aaaccctgcc
    13741 caacaggtac gcgccgcatt ggacacgctg tcgtcccatc ctgacatccg tcttaaacag
    13801 gcttcctcac tgtatatgac cgcgcccgtc ggttacgaca atcagcccga tttcgtcaat
    13861 gccgtctgca ccgtttccac cactctggac ggcattgccc tgcttgccga actcaaccgt
    13921 atcgaggctg atttcggacg cgaacgcagc ttccgcaacg cgccgcgcac attggatttg
    13981 gacattatcg actttgacgg catctccagc gacgaccccc accttaccct gccgcatccg
    14041 cgcgcgcacg aacgcagttt cgtcatacgt cctttggcag aaatcctccc tgattttatt
    14101 ttgggaaaac acggaaaggt taccgaattg tcaaaacagc tcggcaatca aggtatccgc
    14161 cttttgcccg aagaataatc cgccacacgg aaatgccgtc cgaacaccgg tttcagacgg
    14221 catttttcct ttgccgccaa cacgcgtgca aaaaaaatcg ccccttggga aaggggcgca
    14281 aaaggaacac aaaccactac caaaacttta aatctacaac attgcctgcc gcatactgta
    14341 tccgacagga tataaagccc tcactaaatc gtttcgagaa atccaaactt cttcatcgcc
    14401 gacagaaaat ctgcctttct ccggtaccag ctccaacaga accggttgaa ccgccgtatg
    14461 caacttgtcc ttacaccgcc ccagctttct ggacaacgcg gacagggcgc gtttgtaggc
    14521 actatccttg cggtcaagct cccgcgcact gccgacccag ctcaaacgaa ggttgcggct
    14581 ttcatcctca atcagcaaaa tgcctgtttt aacttccccc acctcgggct cgcatgaaag
    14641 cgaaatataa tatttgccat cgatgttgac atacgccgcc tcatgcacct gacagttgca
    14701 gccggaagtg gcaagcagcg aataaacaga atcccgatgc agtttcaaaa tatcaaggga
    14761 aatctgttta aaaatacttt ccggcatttt tccctccttt cgtctgctgc gttgtttact
    14821 ggattgactg cattatacag acatctccca atcttgcaaa tgataattgt tcctattttt
    14881 taaaaaattt ataaattatt gatggatatg tgaattaatt ttaagattta aaatctcttt
    14941 ctcaaaaaaa cacaaaaatg ccgtctgaaa cgtgttcaga cggcattggc tgcacagcca
    15001 gtccgtttaa aatttcacgc ccttatgtaa cgcaacgatc cccgcactca tattatgata
    15061 gtccacactg tcaaatccgg catccagcat catttgcttt aaagtttcct gatcggggtg
    15121 catgcggatg gattcggcga gatattgata actctccgca tctttggcaa tcagtttgcc
    15181 catgaccggc agcagcttga acgaataaaa atcatacgcg ccttcgagcg gcttgtagat
    15241 tttggaaaac tccaacacca gcaatgtccc gccagatttc aaaacacgat acatctcttt
    15301 cagcgcggca tctttatgcg tcatgttccg caagccgaac gccacggaaa ccaagttgaa
    15361 ataattgtca gggaaaggca gtttttccgc atcggcaagc gacacgggca aaatcatgcc
    15421 ttcgttcaac agacggtcgc gcccgacggt cagcatagag gaattaatat cggtcagcca
    15481 aacctcgcct tccttgccga cccgtttcgc ccaaccgcgc gacaaatcgc ccgtaccacc
    15541 cgcaatgtcc aacactttat cgcctttttt caggtgcgcc gtgttgatgg tgaaatgctt
    15601 ccacaccctg tgcagccctg ccgacatcac atcgttcata atgtcgtagt ttttggcgac
    15661 ggagtggaac acttccgcca cttttccggc tttctcatct tcgttgaccg tgctgaaccc
    15721 gaaatgcgtt ttctgtccgc ccatattcta accacctccc tgatgccgcc ctacggcatc
    15781 ccaaacttaa caatctgatt gcttttcaaa acgggaagca cccgccgccg ccaatttgtc
    15841 caaatattcc tgccacatcg catcgtatcc gtatgccagt ctgtgcagat acgcccaatc
    15901 gtaaagaccg ctgtcgtgcc cgtccgaaaa actgattttc agcgcgtact gtccgacaag
    15961 ctgcaaatcc gtgatttgga catccgcctt gccggtctgc aaaacatcct gtcccacacc
    16021 gtgtccgcgc acttccgcac tcggcgaata gacgcgtaaa aattcggcag gcagattttt
    16081 aggttcgtcc ccataagtca aaaccaacac ggtacggttt ttcaacaaac ggatttcaac
    16141 aggaatatta ttcaaaaaac acctcggttc ggcatacaat atccggcagg cgcaggcgtg
    16201 ccttcaaacg gcgcgtattt taacagcttt atcccgatac cgatattcaa acccacattt
    16261 cagacggcat tgatatggac aatctcgtat gggacggcgt tcccgacatc cgcacactcg
    16321 accaaaccat ccgcaaacac gcacacccgc tcaacctgat tgtctgcctc cccgataatc
    16381 agattcccga ttttcaaacc gcacaagatg cttcggacgc ggaatgccgt ctgaagcacc
    16441 gtttggatca ggcaacgcag tgcctccagt tcgacagcat caacctcatc gaacacatcc
    16501 tgcccgatgt ccgcttctgg ctggttcccc cttcacgcac ccgccgcctg cacgaacact
    16561 tccaccacat ttcctggcag accgaagcca tcccgcaaac cgaaagcaaa cccgacaaac
    16621 cctggtttgc acttccacaa acatccgaac ggcaaaaacc ggaacacatc ctcgttatcg
    16681 gagcgggcat atccggcgcg gcaaccgccc acgccttagc atcacacggc atttccgtta
    16741 ccgtattgga agcccgaaaa gccgcccaag ccgccagcgg caaccgccaa gggctgctct
    16801 acgccaaaat ctcgccgcac gacaccgaac aaaccgaact gctgcttgcc ggctacggct
    16861 acaccaaacg cctgctcgga catatcctgc ccgaatccga aacctggggc ggcaacggca
    16921 tcatccacct caattacagc cgcaccgaac aacaacgcaa tcacgaattg ggtttgcaaa
    16981 aacaccataa ccacctctac cgcagcatca cgcaggcaga agccgaaaaa atcgccggta
    17041 tcccactgtc cgtcccatac gaccaccctt catgcggact ctactggcaa cacggcgtat
    17101 ggctcaatcc tcccacattc gtccgcgccc tcctcagcca tccgctgatc ggactgcacg
    17161 aagacacacc cttgaccgac atttcccacg acggggaaaa gtggattgca agcacgccaa
    17221 acggcacatt taccgccaca cacatcatct actgcaccgg cgcgcacagc tcctgcctgc
    17281 ccgaaaccaa cctcgccgcc ctgcctctca ggcaaatacg cggacaaacc ggcctcacac
    17341 catccacccc gttttccgaa caactgcgtt gcgccgtttc aggcgaaagc tacatcagcc
    17401 cgtcgtggca cggactgcac tgctacggcg cgagttttat tcccaacagc agccataccg
    17461 gatggaacga agccgaagaa gcctcaaacc gccaagcatt ggcacagctt agccccgccc
    17521 tcgccgaatc attgtttgcc gccaacccaa acccccaaaa ataccaaggg cacgccgcca
    17581 tacgctgcga cagccccgac caccttcccc tagtcggcgc actcggcgac attgccgcca
    17641 tgcggcagac ctacaccaaa ctcgcgctgg acaaaaacta ccgcatcgac accccatgcc
    17701 catacctgcc taatgcctac gtcaacaccg cccacggcac acgcgggctt gccaccgccc
    17761 ccatctgcgc cgccgccgtt gcagccgaaa tcctaggctt gccccatccc ctctcaaaac
    17821 gcctgcgcca cgccctacac cccaaccgca ccatcatccg cgccatcgtc agaaggaagg
    17881 atctaacccc ttaagccttt gccgcatgcc gtcccgacca cagaaaatca tattcaaata
    17941 aacataccaa tcctgacggt aaaatatcga aaacacccat aacgttttct tcccaaaata
    18001 aggagtaagg aatgcctaac aaattgcctt tgctgtccaa gatgaatgtt ccgtaacatt
    18061 cgaagacttc cttaaccctg ccagccgttt cggtttctgt accccctgtt agataaggaa
    18121 ggaatgaaaa agccctgttg attgtatgaa gtaaagggcg ttaatttttg ttaatcttcc
    18181 cttctcagga actcaatata taaggatgtt aatttttatt tcccttctcg aggact
//
LOCUS       DAWHRA010000030        16998 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWHRA010000030 DAWHRA010000000
VERSION     DAWHRA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.030"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            88..2622
                     /locus_tag="WIB30_000750"
     CDS             88..2622
                     /locus_tag="WIB30_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ3796848.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRISLSAANIEAENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVTLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKSSIHTETVSSSKSTLWQKQAGWGSNIETLQLPSFTGPVAPVLTAPGGYI
                     ADIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQVQLAYDRWDYKQEGLTEAGAA
                     IIALAVTVVTSGAGTGAVLGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKEL
                     GRSSTVKNLVVAAATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNG
                     GSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQD
                     GAIGAAVGEIVGEALVKNTDFSRMSATEIEKAKAKITAYSKLVAGTASAVVGGDVNTA
                     ANAAQIAVENNTLYPRCVGAKCDEFQKEQQKWIRENPEEYREVLLFQTGFIPIIGDIQ
                     SFVQAQTAADHLFALLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQTELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSREWIKQVVKSLPD
                     GSPAKAVVLKANQNGKLKTAIAGVDRQTGKAVILSVKVPSKTNIRR"
     gene            2625..3560
                     /locus_tag="WIB30_000751"
     CDS             2625..3560
                     /locus_tag="WIB30_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ3796849.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESIDYVDEKKGVP
                     LAAMKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            4163..4507
                     /locus_tag="WIB30_000752"
     CDS             4163..4507
                     /locus_tag="WIB30_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796850.1"
                     /translation="MRTHFVISNVPSTPAPLRGRKLTGKLILEVPAQVNPIPQSVLRA
                     AQEENVIIRDTTGRIYNRWWFSIRWLCRCTSCKKRTIDTNRENRGRCVSNCKCKHWIS
                     MDCQFVKAKRRS"
     gene            5075..5533
                     /locus_tag="WIB30_000753"
     CDS             5075..5533
                     /locus_tag="WIB30_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796851.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     SYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            5532..5666
                     /locus_tag="WIB30_000754"
                     /pseudo
     CDS             5532..5666
                     /locus_tag="WIB30_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            5745..6026
                     /locus_tag="WIB30_000755"
     CDS             5745..6026
                     /locus_tag="WIB30_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3796852.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(6047..7273)
                     /locus_tag="WIB30_000756"
     CDS             complement(6047..7273)
                     /locus_tag="WIB30_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ3796853.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(7288..7713)
                     /locus_tag="WIB30_000757"
     CDS             complement(7288..7713)
                     /locus_tag="WIB30_000757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796854.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSECGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            7818..8477
                     /gene="queC"
                     /locus_tag="WIB30_000758"
     CDS             7818..8477
                     /gene="queC"
                     /locus_tag="WIB30_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ3796855.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERASWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            8505..9023
                     /locus_tag="WIB30_000759"
     CDS             8505..9023
                     /locus_tag="WIB30_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ3796856.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            9030..9452
                     /gene="queD"
                     /locus_tag="WIB30_000760"
     CDS             9030..9452
                     /gene="queD"
                     /locus_tag="WIB30_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ3796857.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDLVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            9721..10092
                     /locus_tag="WIB30_000761"
     CDS             9721..10092
                     /locus_tag="WIB30_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ3796858.1"
                     /translation="MKLLSTLLVLLVAAEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            10089..10727
                     /locus_tag="WIB30_000762"
     CDS             10089..10727
                     /locus_tag="WIB30_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ3796859.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGRLKAYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQNGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(11062..11138)
                     /locus_tag="WIB30_000763"
     tRNA            complement(11062..11138)
                     /locus_tag="WIB30_000763"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11102..11104),aa:Pro,seq:cgg)
     gene            complement(11366..12451)
                     /gene="nagZ"
                     /locus_tag="WIB30_000764"
     CDS             complement(11366..12451)
                     /gene="nagZ"
                     /locus_tag="WIB30_000764"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ3796860.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEERQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGQCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(12508..14028)
                     /locus_tag="WIB30_000765"
     CDS             complement(12508..14028)
                     /locus_tag="WIB30_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ3796861.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(14230..15729)
                     /locus_tag="WIB30_000766"
     CDS             complement(14230..15729)
                     /locus_tag="WIB30_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ3796862.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            complement(15867..16496)
                     /gene="nth"
                     /locus_tag="WIB30_000767"
     CDS             complement(15867..16496)
                     /gene="nth"
                     /locus_tag="WIB30_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ3796863.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(16542..16955)
                     /locus_tag="WIB30_000768"
     CDS             complement(16542..16955)
                     /locus_tag="WIB30_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ3796864.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         4411 a         4204 c         4431 g         3952 t
ORIGIN      
        1 tgccgtctga aaagctcaca atttttcaga cggcatttgt tatgcaagta aatattcaga
       61 ttccctatat actgcccaga cgcgtgcgtg ctgaagacac cccctacgct tgctgcagaa
      121 ctttcgggta aaaccggtgt gagcattagc gcaccgtatg ccaatgagaa cagccgcatc
      181 agtctgagtg cggcaaacat cgaagctgaa aacggcaaaa tcaaaattca atcttacggt
      241 gaccaatatt actatgcgag acagagcgaa ctctatacct ttgaacgccg cagctacaaa
      301 actggcaaat ggtacaaccg caaacacatt accgaagtca aagaacacaa aaacgccaag
      361 cccgacgcag taaccctcag cgcatcccaa ggcatcgaca tcaaatctgg tggcagcatc
      421 gacgcctacg ccaccgcatt cgatgccccc aaaggcagca ttaacatcga agccgggcgg
      481 aaattgacac tctatgccgt agaagagctc aactacgaca aactagacag ccaaaaaagg
      541 cgcagatttc tcggcatcag ctacagcaaa gcacacgaca ccaccaccca agtcatgaaa
      601 accgcgctgc cctcaagggt agttgcagaa tctgccaatc tgcaatcagg ttgggatacc
      661 aaactgcaag gcacacagtt tgaaaccaca ctgggtggcg caaccatacg cgcaggcgta
      721 ggcgagcagg cacgggcaga tgccaagatt atcctcgaag ggatcaaaag cagcatccac
      781 acagaaaccg tgagcagcag caaatctact ctatggcaaa aacaggcagg atggggcagt
      841 aacatcgaaa ccttgcaatt gccgagtttc accggtcccg ttgcgcccgt actgaccgct
      901 cccggcggct atatcgccga catccccaaa ggcaacctca aaaccgaaat cgaaaaactc
      961 gccaaacagc ccgagtatgc ttatttgaaa caacttcaag ttgcgaaaaa catcaactgg
     1021 aatcaggtgc agcttgctta cgacagatgg gactacaaac aggagggctt aaccgaagca
     1081 ggtgcggcga ttatcgcact ggccgttacc gtggtcacct caggcgcagg aaccggagcc
     1141 gtattgggat taaacggtgc ggccgccgcc gcaaccgatg cagcattcgc ctctttggcc
     1201 agccaggctt ccgtatcgtt catcaacaac aaaggcgatg tcggcaaaac cctgaaagag
     1261 ctgggcagaa gcagcacggt gaaaaatctg gtggttgccg ccgctaccgc aggcgtagcc
     1321 gacaaaatcg gcgcttcggc actgaacaat gtcagcgata agcagtggat caacaacctg
     1381 accgtcaacc tagccaatgc gggcagtgcc gcactgatta ataccgctgt caacggcggc
     1441 agcctgaaag acaatctgga agcgaatatc cttgcggctt tggtgaatac tgcgcatgga
     1501 gaggcagcca gtaaaatcaa acagttggat cagcactaca ttgcccataa gattgcccat
     1561 gccatagcgg gctgtgcggc agcggcggcg aataagggca agtgtcagga tggtgcgata
     1621 ggcgctgcag tcggtgagat tgttggtgag gctttggtta agaatactga tttcagtcgt
     1681 atgagtgcga ccgaaatcga aaaagctaaa gcgaagatta ctgcctattc aaaactggtt
     1741 gccggcactg cgtctgccgt tgtaggcggg gatgtgaata cagcggcgaa tgcggcacag
     1801 atagcggtgg agaataatac tttgtatcct agatgcgttg gtgcaaagtg tgatgaattt
     1861 caaaaggaac aacaaaaatg gatacgtgaa aatcctgaag aatatcgaga agttttgctt
     1921 tttcagacag gatttattcc aattatcggt gatatacaga gttttgtaca agcacagacc
     1981 gctgccgatc acctgtttgc tttgctgggt gtggttccgg gtatcggtga atcgatacag
     2041 gcctataaag tagcgaaagc ggcaaaaaat ttacaaggca tgaaaaaagc cttggacaag
     2101 gcagcaaccg ttgccactgc acagggctat gtcagtaaaa ccaaaatcaa aatcggtcaa
     2161 actgaattaa gggttactgc agcaactgac aaacaattgc tgaaagctat tggcgaagga
     2221 agggacacga caggtaaaat gaccgagcag ttatttgact ctttagctaa acaaaatggc
     2281 ttcagagtgc tttcgggcgg caaatacggc ggaaataacg gttttgatca tgtatggcag
     2341 gctgccgatg gtagtgttgt tttgattgta gaaagtaagc agattaggaa cggtacggta
     2401 cagctgaatc cgaatggtgc gggtggatat acgcagatga gtcgtgaatg gattaaacaa
     2461 gttgtaaaaa gtttacctga tggtagtcct gctaaggcag ttgtcttaaa agcaaatcag
     2521 aacggcaaat taaaaacggc aatagcaggc gttgatcgtc aaacaggtaa ggccgttatt
     2581 ctttctgtca aagttccttc taaaaccaat ataaggagat aacaatgggg cacaatatga
     2641 tgaccaccca aaaatggtat gaacatatta ctaatgtaat cataggcaat actgctaatt
     2701 tcaatagcgg ttgccccgaa tctatagatt atgtagatga aaaaaaaggc gtgccgcttg
     2761 cagcgatgaa atacatttta atgtacactg aagctgcggc ttcccatgcc tatctatttg
     2821 aacatgatct taagaaattc aagcaatatg cttatgttgc aggaaagttg ggtattttgc
     2881 agagtgtaga tgatgaagac cccgaaccct tcttctttcc ctgcgacatg ctcaacattc
     2941 aagatccgat gtttctgatg ctgatgagcg acagcccgca gctgcgcgag tttctggtgc
     3001 gcaatatcga caacatcgcc aacgatacag aagcctttat aaaccgctac gacctcaacc
     3061 gtcatatgat ttacaatact ctgctgatgg tggagggtaa gcagcttgat cggttgaaac
     3121 aacgtagcga gaaagtcttg gcgcatccca cccctagcaa atggctgcaa aagcggttgt
     3181 acgattaccg cttcttcctc gctttcgccg aacaggatgc cgaggcgatg aaggccgcct
     3241 tagagccgct tttcgataaa aaaaccgcgc gtatggctgc caaagaaaca ttgtcctatt
     3301 tcgatttcta cctgcagccg caaatcgtta cctacgccaa aatcgcatcc atgcacggtt
     3361 tcgatttggg catagatcaa gaaatcgcac cgagggattt gattgtttac gatccgctgc
     3421 cggcagacga atatcaagac atcttcgatt ttatgaaaca gtatgacttg tcttacccgt
     3481 atgaatatct gcaggattgg atagattact atacgttcaa aaccgataag ctggtatttg
     3541 gtaacgcgaa gcgagagtga gccgtaaaac tctgagctcc tgttttatag attacaactt
     3601 taggccgtct taaagctgaa agattttcga aagctataaa ttgaagccct tccatagtac
     3661 atagatctgt gttgtggcga ggctttacca cgctgattgc cggagaagaa ctcaacctgc
     3721 tggcaaaaca aggcatgaga tctttgcaat aacatgagtt gagacctttg caaaaaagcc
     3781 cttccccgac atccgaaacc caaacacagg atttcggctg ctttcgtacc aaatacctcc
     3841 taattttacc caaatatccc cttaatcctc cccggatacc cgataatcag gcatccgggc
     3901 tgccttttag gcggcgcggg cgcacttagc ctgttggcgg ccttcaacag gttgagacct
     3961 ttgcaataac ataggttact aaaattttat gctcaatctc attttcaaaa tgcaaaactt
     4021 ttctgatttt tcctactttt tgctcaatat taggaaggtt ttaggcaatt gaaaattttt
     4081 tggcgcattt ttatgcgtca aatttcgtta acagactatt tttgcaaagg tctcaggttc
     4141 aaacacatcg ccttcaggtg gtttgcgtac tcactttgtc atttccaatg ttccaagtac
     4201 acctgctccg ctaagaggaa gaaaacttac aggaaaactt attttagaag ttcctgctca
     4261 ggtcaatcca attccacaat ctgtattaag ggcggcacaa gaagaaaatg ttatcattag
     4321 agatacaaca ggaaggattt acaataggtg gtggtttagt attaggtggt tgtgcaggtg
     4381 cacatcttgc aagaaaagaa ccattgatac taacagggaa aacaggggca ggtgcgtcag
     4441 caattgcaaa tgcaagcatt ggatatcaat ggactgtcaa tttgtcaaag ccaaaagaag
     4501 gagctaaata ataatgcatt cccactatat atttggtatt ttgatgattt catatgtttt
     4561 cgcaatgtta tttaatttta taatatcata taaaatattt aaagaagaaa aattaattaa
     4621 tggttttttt gattttctaa ttaaatcaag ctaccttaac tttaaatatt tcaatatatt
     4681 atttggaaaa taaaaaatct caaatatttt ttatttgaaa ttattaagaa ttaatctggc
     4741 gttgggggtt tttatcttat ccttaataat tataaatatt ttttgttttt agtaaaaata
     4801 tggtacagat atgtacagct agctttgttt cagtaaggta taactgtata taatactcag
     4861 atttttcacg ttgggctata catggaaata tatctgtgat taaagatgtt aatggtaagt
     4921 atcgattagc acctgaaaag catgatttta aaatgcattc ctttgggggg agaaaaaagt
     4981 aatgtaaaaa caatatttag aaatatggaa actataattg gtagcccagg gtaaggggta
     5041 cctttcagga ttgaatttaa aggagaggta aatattgtta actaagttga aaattttgct
     5101 atttttgttc ttatttgttt ttgtattggc tattaatttg cttttcttct tttttagttc
     5161 ggatatcgag agtttcggga actatcagtt tgaatatgtt tacgataaag gttggcctgc
     5221 taattatatt ttagtcatga aagatggaaa tgaagggaat tttgataaaa taatatccgg
     5281 attggtttta gaatattata aggaggatga taacatttat ttttcttata ttgacgggca
     5341 aggatttgct tcagactctt gctattacaa accggaaatt tcatatggaa aaattatttt
     5401 aaataaaaat catatcatta atattaatag catggaaaaa aataattttc tttcagaaga
     5461 taaaataatg aagggaacaa gaaattggct agcagaccct aaaaataaat gtaatataca
     5521 gactctagac taaacgtgtc ttgcgaaaat acaacggaat cgattgcaaa tctttcccgc
     5581 tgttcttgaa agaatgcgaa tttcgattta acttcggcac accgtctcaa cagcttaaaa
     5641 tcctgcggga ttggtgtggg atttagggct aatctagtac agccccttgt tttttcgata
     5701 cggaaccgga tagaggaaaa atcgaacatt gcgcctgcct tgctatgatt cacgaagaaa
     5761 tctccgccat gcctatgggc tatgaaacct taatcggcga tatgggcagc gcactgtcag
     5821 gcggacaaaa acaacgcatc gtattggcgc gggccttata ttgcgaaccg aaaatcctat
     5881 ttttagatga agcgaccagc catttggata ttgccaatga aaaagcagtc aatgcaaact
     5941 tgaatggctt gtctatcata aaaattatgg cggcacacag aaaggaaacg gtggaatcag
     6001 cagataggaa aatgtcttta ggataaaaat acagtttcaa aaatactcaa gattgctgct
     6061 gttttttcgc ctgagcgtca aactctgcca gcgtcatgtt caaagtctgc aaacacggca
     6121 tcattaccgc atcgacagct tggttcacat gatccctttc cacaggcaac ggacggtaaa
     6181 cgaagagctt gaagagttcg ttcaactcaa tcgaatccgc ccccgttttc aacacccaac
     6241 cctgtctgcc ggaatagatg tagccgtgcc gcgccagctt ttccaaaagc tcgcccaact
     6301 cgtcgtagcc catattgata tgccgtctga actcctgaac aggcaaggct ttgccttctt
     6361 tttgcgccgc atccagaagc agcaggattt tcaacacgtc gtcaaaccgt ccgcgcgagt
     6421 cgaagcccct gcggaacgct tctccctgcc agtaggagag tgaagaagtc agcaccgcgc
     6481 cgcccaagac cagcgtccac aacaggttca gccacaacag aaaaaacggc acggcggcaa
     6541 acgcgccgta aatcgagcgg tagccgtcga aattgcccat ataccaagtg aagagggagc
     6601 gcgcggtttc cagacaaaac gctgttgcca aagccccgac aaacgcctgc cgcgcgggaa
     6661 cgaagcggtt tggcacgaag cggtacagcc cccacagcaa aagcgtcatg aaggtcagcg
     6721 tcgccgccgt tcgcaacgcg cccgaccact gcggcgcacc tgaggcaagc gcggcatcct
     6781 gtaccgagcc gaccataaag gaaatgccca cgcccaaaga cagcggcccg aacgtcagta
     6841 aagcccaata gacgagaaac tgcatcatcc acggacgctg ggaattgacc cgccagatgc
     6901 ggttgaacgt attgtctatc gtccgaatca gcatcagcga ggtaacgacc agcatcacgc
     6961 tgccgattgc cgtcagccgg ttcgcctgct cgcggaacgc attgatatag tcgaacacca
     7021 tgtccgcgcc ctgcggcaca atggtttggt tgacgaagga gacgaacgaa tccgaccagc
     7081 ggtcgaacac ggggaaaatc gaagcgaccg ccaccatcac ggtcagcacg gggacgagtg
     7141 ccagcagcgt cgtaaacgtc atgcttgccg ccgcctgcgg tacgcgttct tcatcaaagc
     7201 ggcggacgac gaaccatgca aacgcacaga ttttattgtc tgccaaacct tgcaaacgtt
     7261 gtaaaaaggt cataatttct tgcccggtca gtaagttggg cattgatgcc cgatgttata
     7321 gccaattttg ccgtcaggaa caaatgcctg aactgcggct gtttcagacg gcatcggaac
     7381 aactgttatg ccgtctgaag accgaaccat tttaacggaa tccgcccatg aacccaaatc
     7441 cccctcaaaa tcctcgtcct ctactatttc caaaacggca gcacccgcaa tcccgcactc
     7501 cgaatcgctc gcggcatcga cagcgttgaa ggttgcgaag ccgtattgcg caccgtcccc
     7561 aaagtctccg ccgtctgcga agccgtcaaa aaagatattc ccgacagcgg ctcccgtcct
     7621 gaccgccgaa gaaaacaata tcgccttcgc acaaagcaaa cgcttggcgg aactcgccgt
     7681 caagtcggca taagccgcgt gttcagacgg catggcgttc agatgccgtc tgaacgcgtt
     7741 tgcctgtata atccgcgtct ttactgtcca acttcgcggt tcgcaaacct cccgcgttac
     7801 caaaactagg attcgatatg tcaaaccaaa aagccttggt catcttttcg ggcggtcagg
     7861 attcgaccac ctgcctgatt caggcaatcc aaatctacgg gcgcgaaaac gtccaagcca
     7921 ttactttcca atacgggcaa cgccatgccg tcgagctgga acgtgccagc tggattgcgc
     7981 aggatttggg cgtcaaacaa accgtactcg acttgagcct gatgcggcag attacgcaca
     8041 atgccctgat ggacgacacc gccgccatcg aaactgccga aaacggcgtt ccgaatacct
     8101 ttgtagacgg ccgcaacgcg cttttcctgc tctatgccgc gatttacgcc aaagggcagg
     8161 ggatacggca catcatcgcg ggcgtgtgcg aaaccgactt ctccggctat cctgactgcc
     8221 gcgacgtgtt cgtcaaatcc atgaacgtta cccttaatct ggcgatggac tatgatttcc
     8281 aaatccacac gccgctgatg tatctgacca aggcgcaaac ttgggcgttg gcggacgaaa
     8341 tgggcgcgct ggactatatc cgcgagcaaa cccacacctg ctataacggc atcgtcggcg
     8401 gctgccgcga atgcccgagc tgtatcttgc gcgaacgcgg gctggcggaa tatctggaaa
     8461 gtaaaaaggc cgtctgaaca cgcgcaaacc ataaggaata cgatatgccc aagctccata
     8521 tgttttacct cggcggcaat gccggcaggt cgaatatcga agtgcacgac atccaatttg
     8581 ccgtgtgcga cgactaccgt gaagccgtcc ccgcactcaa agccgcgtgg ttcggcgatg
     8641 cggacaaaat ccacatcgac ggctggcagg ttgtcgaatg ggcggacggt tacgacatcg
     8701 ccgtatccga aacgcccaaa acgaaaatgc cgtctgaaca cgccccgcgc ctgtatttcg
     8761 ccaatgtcgg cggttatcgc gcagggcagc tcgccgaagc gcacgctttt gggctgttcg
     8821 ccgccgccac gcctgccgaa gccaaacaaa aagccctgca aaccctgttg accgacagct
     8881 atgttcagca acataaagac aacttaaaag acgtggacaa cctgcttgcg ctcgaccgca
     8941 tcggcaattt ccatatccgc ctgaccccga atccgcacgg caaacccgcc gaaatcggct
     9001 ttcaaggcta tttgcccatt tgagaaccca tgaaaatcac caaaatcttc accttcgact
     9061 cctcgcatat gctcgacggg catgacggca aatgccaaaa cctgcacggg catacctaca
     9121 aactcgaaat caccgtttca gacgacctcg tcagaggcgg gccgaaagac ggtatggtga
     9181 tggactttac cgacttgaaa gccattgtca aacaacacat taccgacccc ttcgaccacg
     9241 ccttcatcta ccacggcggc aacgggcgcg aatctcaaat cgccgcactc ttggaaggct
     9301 ggaacatgaa aaccctgcgc ctgccctgcc gcaccactgc cgaaaatatg gcggtcgaaa
     9361 tgtacgaccg tctgaaaaac gcggggctga acgtgtgccg cgtcaaattg tgggaaacgc
     9421 cgacatcgtg tgcggagtat gaaggggagt agggaatatc ttgaacgtat cgatatagta
     9481 aattccaata agacatgccc aaccgcgtca ttcccgcgca ggcgggaatc cagaccttgg
     9541 tttttcagga atatttaaaa attgcagcaa ttccaaatct ttggattccc gcctgcgcgg
     9601 aaaggacggt ttagagcgtc cttatttgaa tttaccgtaa aacggttttt tctcctgtac
     9661 ggattccccg ttttttcaga cgaccttcca tatcaaatac acccattaaa aggaataccc
     9721 atgaaacttc tctccaccct cttagtcctc cttgtcgccg ccgaacattt ctacatcgcc
     9781 tggcttgaaa tgacgcagat tcccagcgaa aaagcggcgg aaatattcaa gctgccttat
     9841 gaatttatgg aacaaaatcg cgtgcagacc ctgttcggca accaagggct gtataacggc
     9901 tttctcggca tcgggctggt gtggtcgcgg tttgccgcgc cggataacgc ggtgtacggc
     9961 gcgacggtac tgtttcttgg cttcgtcctg attgccgccg cgtggggcgc gttctcttcc
    10021 ggcaacaaag gcatactcgt caaacaaggt ttgcccgcat ttttggcagc ggcggcggtg
    10081 ttggcggtat gaaaaaaatc agcgtcgccc ccgaaaatcc ccaataccgc atcgtcgaaa
    10141 tctttgaaag cctgcaaggc gagggctgga acacgggcat gcccgccgtg ttcgtccgcc
    10201 tgggcaaatg caacctcgcc tgcggctggt gtgataccga ttatttgaca ttcggcatga
    10261 tgagcctgtc cgacatctta ggccgtctga aagcctatgc cgcccgcaac atcatcatca
    10321 ccggcggcga accgaccatc cagccgcatc tcgatatgct gctggacatg ctcaaggcgg
    10381 aaggttattt cctctgtatc gaaaccaacg gactcaatcc cgcgccgccg caaatcgact
    10441 atgtcgccac cagccccaaa gcctgctatg ccgccaaata cgaaaaaagc tgtatcgaaa
    10501 cagccgacga agtgcggatt gttgccgacg gcgatgtcgt tgcgttctgt gaaaatatgg
    10561 aacgcaaaat ccgtgcgcat cattactacc tttcgccctg cgaacaaaac ggtgcaatga
    10621 acatctacga caccatccgc caaatcggta ttctgaacag ccgccccgac gcgccggtgc
    10681 attggcagtt gagcgtgcag acgcacaaat gggcgggaat agagtagttt aagcagtgta
    10741 actcaaaggg acggcgtacg gttttaccga tgtttgacat acggggaaag tgtgccgctt
    10801 ctgcgtggaa atgccggcat ttccaccgcc caatcaggac ggagccttac tgaataagat
    10861 gctgccgttg ggtacaagct cggcttccta aattccgatg gtcttttgaa ccttgccgat
    10921 actctgtgcc agtgcgcgca aatggcaggg ttagggaaaa cgaaatgccg tctgaaacag
    10981 cattctgttt cagacggcat ttttctgttg ccgccaaaag gaaaaaccgc tccggcaatc
    11041 tatgccgagg cggtttggat ttggtcggaa tgagaggatt cgaacctccg accccttcgt
    11101 cccgaacgaa gtgcgctacc gggctgcgct acattccgaa ttaagtaagg cgtgattata
    11161 gcgcaaaaaa tacggcgtgc ctataccgtt ttgccttttt gccgcgtgtc gggcggattt
    11221 aaaggagtgt gtttgaatat agtgttgata atcatcatta tctttaagta attcaataag
    11281 ataactttct acctgaccga aaaaatcatt gcctttccct gacaaacggt tgatgaaatc
    11341 ggcagattgt tgaaacgcag ccggtttaaa aggcttcgcc gacttttacg ccgcccgccg
    11401 tgtcttgcgg cgaggcaagg ccggcaacaa aggcttgcgc cgcttggaaa tccgccgtct
    11461 gcataacggc ttgcgcggcg gcactgccga gcgtgttggc catgtattgc caacgttgcg
    11521 ccaaagcggg attgtcagga atgcggaaat catcgcgcag ttcgtccaca aggtcgggac
    11581 ggttgcagac gaggacgatg tcgcaacctg cctcaaagga aatgcgggcg cgttctttga
    11641 tgccgcctgc cccgcacgcg ccctccatag ttaaatcgtc cgagaaaatc acgcctttga
    11701 acccgatgtc gcggcgcaaa atttgtttga gccagatttc ggaaaaccct gcgggctttg
    11761 tgtccacttg tggataaacg acgtgggcgg gcataaccgc cgccatacct tcgcggctca
    11821 taatgtggaa gggggcgagg tcggcggctt cgagttcgga caggctgcgc ctgtcttccg
    11881 gcaagaccag atggctgtct ccttcgacaa atccgtgtcc gggaaaatgt ttgccgcagg
    11941 atttcatacc gccttttgcc aaaccttttt gaagggcgag ggcgaggcgt gcgaccgctt
    12001 cggggttgcg gtggaagctg cggttgccga tgacggcgca ttgtccccag tccaagtcga
    12061 gaacgggggt gaaggagagg tctatgccgc aggcggaaag ctcggttgcc aacacccggc
    12121 cgacttgtcc ggcggcggtt tcggcggcgg acgcgccgtc tttgtcccaa atttgcccta
    12181 aaacgttcat tgcgggcagg cgggtgaagc cttcgatgaa acgttgcacc ctgccgcctt
    12241 cgtgatcgac ggcgataatg agttcgggtg tgcgcagggc tttgatttcg gcggtgagtg
    12301 ttttgagttg ttcgatgttt tggaagttgc ggcggaagag gatgatgccg cctacggcgg
    12361 gatcgagcag gcgttgcctt tcttctccgg tcaggcggaa ggcggcaatg tctgccatga
    12421 cggggccgcg cggaatatgg gggacggtca ttgcggtttg ctccaaaaag cttcagacgg
    12481 catatgccgt ctgaacaggg aaaggggtca ggcgttggcg cgttttttat ctttcaacag
    12541 aaaaatcagc accgccaata caatgcctgt cgtgccaaag cccaacagcg cggattttgt
    12601 cagacccaat gcgaggtagc ccgatgcggc ggcggcggca acggttaagg cgtaaggcag
    12661 ttgcgaggta acgtggtcga tgtggttgca gcgcgcgccg gtggacgaca ggatggtcgt
    12721 gtcggaaatg ggcgagcagt ggtcgccgca taccgccccc gccattactg cggacataca
    12781 cgggataatc agcgcgggtt cgactttgac cgccatggcg gcggcaatcg gcagcataat
    12841 gccgaacgtc ccccagcttg tgcctgtggc aaacgccatc acgctggcga gcaggaagag
    12901 gatgacgggc aggaagccgg gatggatgtt gcccgcaacc agtgtggaga ggtaatcgcc
    12961 ggtgtgcatt tcgccgacaa ccgtactgat gagccaagcg aggattaaaa tggcgattgc
    13021 gccgaacata gatttcgcac cctgccaaac ggctttggga tagtcggcgg ttttaatcgt
    13081 gccgagcgtg cagagaacga cggcaaggac gccgcaagtg ccgccgaata ccagcgaagt
    13141 gtttacgtcc gtgttttcaa atgcccccaa aatgctgaag gtttcgcttg cctgcgcgcc
    13201 ggtgtagatc atggcggaaa ccgttgaggc gattaaggcc aaaacgggaa taatcagtgc
    13261 gtaaacacga cctttggtag cgtctgaaac ggcagtttca tcgtgggctt cgttcaacgc
    13321 ggcttgttcg aaacgtgcca tcgagccgat gtcgaaggaa aaccatgcga cgacgaacac
    13381 cataatcagg gcaaacagtg cgtaatagtt catcaggctc atggcgacaa acgtccccat
    13441 cggcgtgtat tcggtgattt tgtaggtaac gagcagtccg gcaagcgtgg cgataatcga
    13501 cgcgccccag cttgaaacgg gcatcagcac gcacataggc gcggcagtgg agtcgaggat
    13561 gtaggcgagt ttggcgcggg aaactttaaa cttgtcggta acggggcggg caatcgcacc
    13621 gacggcgaga ctgtggaaat agtcgtcgat aaaggttacg aacacgaggc aggcggtcag
    13681 cattttcgcg ccgcgccggt ttttaatgtg ccgttttgcc cagtcggcaa acgcctgatt
    13741 gctgccggag taggtcagca gggaagtaaa aatacccaaa agtatcagga aaaccaagat
    13801 ttttggtttg cccagcgacc aatcgccgtc tgaccaagcc aagccgacga ccatgtcttt
    13861 caggtgtgtc agaccgtcga cggggttgcc gccgaccaaa aaggcaacgc cgaccagaat
    13921 accgatgcct aaagacagca gtacgcggcg ggtaatgacg gcaagtgcca gtgccaaaaa
    13981 gggtggcaca accgagaaaa atgaatgtga atagtcgatc agctgcatgg ttatgggggt
    14041 gttaagcgtc cggatgggag cgtatctgtc cgcctccggt ttgggttttg ttggcaaaat
    14101 gggcggaaat attttttgtc gtaaaaaata tttgtttaaa atcaaccaac tgatttttgt
    14161 aaaatgcccg ttaatcggta ttgacgggca ttttatcatt taaaaaatat tttggttaaa
    14221 ttatgtgtgt tattgcaggt ttaatgcgat aaacagcgtg ttgccacggc gcatgatcag
    14281 cagggggacg tttttgcctg ccttgtccat agctttgcgg aaaccggctt cgtcattgac
    14341 ggggacttgc ccgacggcaa gaatttcgtc gccgcgcctc aagcctgcgc gttctgccgc
    14401 gtcggaaacc cgtacgacga cgaggtgtcc gccgctgctg tcggtatgtg tctgaagggt
    14461 aatgcctgcg gattcgaccg agaacgtacc ggattgctgt tcggtgtagg gggcttcatc
    14521 tgttttggat gatgcgccga tatgctcggc ggcgttgccc agcttgactt tgattgtgat
    14581 ttcttcgcct ttgcgccata cgccgaggct gacttctttt cccggcgtaa tggcgccgac
    14641 cataacggga aggtcgccgg aagaacgtat ttctccgccg tcgaggctga ggacgatgtc
    14701 gcccgcctgc aggccggcac gttctgcggg gctgccgggc aggattttgg caatcagtgc
    14761 gccgccggct ttgtccaaac cgaacgattg tgccaaaccg taggatactt cttgaataat
    14821 cacgcccagt tgtccgcgtt ggactttgcc ggtgtttttc agctgttcgg cgacattcat
    14881 ggcaacgtca atcgggatgg cgaaggaaat gcccatgaat ccgccgctgc ggctgtatat
    14941 ttgcgagttg atgccgacga cctgtccttt taagttgaac agcgggccgc cggagttgcc
    15001 cggattgatg gcaacgtcgg tttggatgaa aggtgtgtag ctttcgttgg gcaggcttct
    15061 gcctttggcg gacacgatgc cggcggtcac gctgttgtcg aagccgaagg gcgcgccgat
    15121 ggcagcgacc cattcgcctg gtttcaaatc tttgggattg ccgattttga cgacgggcag
    15181 ctcttccgtt gcgtcgattt tcagaagggc gacatcggat tggacatccg aaccgatgag
    15241 tttggcggta tattcgcgct tgtcgttgag caggactttg atactgccca tgccggtaac
    15301 gacgtgcgta ttggtcagaa tatagccgtc tttgctgatg atgaagcccg aaccgaagtt
    15361 caatccgccg tcatctgctt cttcttgggg gatttcgggc atgttcggga cgaggcgttt
    15421 gaaaaattcg tagaacgggt cgctgtcggc aagcgggtcg gaatcggttt cggcattgct
    15481 gctgccgttt tgggtgcgcg gggcgggggc tgcctgaata ttgacgactg ccgggccttc
    15541 gctttgaacc agttggacaa agtcgggcag cagcatactg acgctgccgt cgtctttggt
    15601 gtgtttgatg cgttctacga aggatgcttc ttttttgtcc gcaccgaaaa agctgccggc
    15661 tttgtcgcag cctgccagcg aggcggcaca cagtgctgcc aaagcgaggt attggtattt
    15721 tttgaacacg ttttgtcctt tgtcggatgc cggtaccggc tttaatgccg tctgaagcgc
    15781 attttgtcgg cttcagacgg cataggttga aattctacaa cgtccgtccg aattttcaag
    15841 cgtttcattt tgaagggcgg cggcagtcag gctttggcgg gatattcgca caaatcgttg
    15901 atgatgcagg tttggcattg cggtttgagt gccttgcagg tgtagcgtcc gtgcaaaatc
    15961 agccagtggt gcgcgtccat cagaaattct ttaggaatga agcgcatcag tttgtcttcg
    16021 acttcgcgca catctttccc gggtgcgatt ttggttcggt tggacacgcg gaaaatatgc
    16081 gtatcgaccg ccatgacggg gtgtccgaac gctgtgttca ataccacgtt tgccgttttg
    16141 cgccccacgc ccggcaacga ttccaaagcc tcgcggtctt ccggcacttc gcccttgtat
    16201 ttttccagca ggatgcggca ggtctgcatg atgtgcttgg acttggtttt atacaacccg
    16261 atggttttcg tgtattccat cacgccgtcc aagcccaaat ccagcattgc ctgcggcgta
    16321 tcggcaaccg gaaacagctt cgccgtcgcc ttgtttacgc cgacatcggt cgcctgcgct
    16381 gaaagcagaa cggcgattaa aagctcgaaa ggggagttga aattcagctc ggtggtcgga
    16441 tgggggttgg cggcgcggaa gcgttcgaaa atttcttggc ggatgtgtct gttcattttt
    16501 ttgtgcagtc ggtttgtgcg gtcggcatta taacgcacgg ttcaggcggc gtaatattgc
    16561 attccccaca gaatgaaggc gtaacgcgcc gttttgccga taaccagcat cagcccgctt
    16621 gtccacggat tcaaccgcag ccagccggcg gcaagcggca gtgcgtcgcc gacgacgggc
    16681 agccaggcaa acggaagcag ccaaataccg aaacgccgca tcagattcag tgttttttca
    16741 gacggcattt ttcgggaagg cagcaaacgt cccatccaat aggaaaccat actgcccaat
    16801 ccgttggcaa ggccggcgca cagcaacgcg ccgtatgcgt gttcgggaaa gcggcggacg
    16861 aacagggcaa aggcggcttc ggatgtgccg ggcaggaggg tggcggaagt gaaggcggaa
    16921 aaggcgagcg cggcgtaggc gtaggcgggc atcattgcaa acagtctcaa acaggcaaaa
    16981 atcggcgacg gattgtac
//
LOCUS       DAWHRA010000031        16984 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWHRA010000031 DAWHRA010000000
VERSION     DAWHRA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16984
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.031"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(52..717)
                     /locus_tag="WIB30_000769"
     CDS             complement(52..717)
                     /locus_tag="WIB30_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ3796865.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYAEMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(917..1813)
                     /locus_tag="WIB30_000770"
     CDS             complement(917..1813)
                     /locus_tag="WIB30_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ3796866.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVARLTMTVYLPAEQKGTHMS
                     RFVALMEQHSEALDFAQLHRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEMKDGAYGYSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            1900..3057
                     /locus_tag="WIB30_000771"
     CDS             1900..3057
                     /locus_tag="WIB30_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ3796867.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFTLHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKATGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPMDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(3120..4121)
                     /gene="hemB"
                     /locus_tag="WIB30_000772"
     CDS             complement(3120..4121)
                     /gene="hemB"
                     /locus_tag="WIB30_000772"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ3796868.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAEEAYNPE
                     GLVPAAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(4304..4588)
                     /gene="yhbY"
                     /locus_tag="WIB30_000773"
     CDS             complement(4304..4588)
                     /gene="yhbY"
                     /locus_tag="WIB30_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ3796869.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            4698..5318
                     /locus_tag="WIB30_000774"
     CDS             4698..5318
                     /locus_tag="WIB30_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ3796870.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLAGPSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSLYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            5382..7349
                     /gene="ftsH"
                     /locus_tag="WIB30_000775"
     CDS             5382..7349
                     /gene="ftsH"
                     /locus_tag="WIB30_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ3796871.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADTAEDN
                     APHAPAREETEAPAPADTASTESEQQPENKA"
     gene            7570..8001
                     /locus_tag="WIB30_000776"
     CDS             7570..8001
                     /locus_tag="WIB30_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ3796872.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            7994..8272
                     /locus_tag="WIB30_000777"
     CDS             7994..8272
                     /locus_tag="WIB30_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ3796873.1"
                     /translation="MLEIEIVYGLSDKQVLKNMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            8325..8903
                     /gene="pth"
                     /locus_tag="WIB30_000778"
     CDS             8325..8903
                     /gene="pth"
                     /locus_tag="WIB30_000778"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ3796874.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            8964..9170
                     /locus_tag="WIB30_000779"
     CDS             8964..9170
                     /locus_tag="WIB30_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ3796875.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            9167..9529
                     /locus_tag="WIB30_000780"
     CDS             9167..9529
                     /locus_tag="WIB30_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ3796876.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            9760..11175
                     /locus_tag="WIB30_000781"
     CDS             9760..11175
                     /locus_tag="WIB30_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ3796877.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            11294..11803
                     /locus_tag="WIB30_000782"
     CDS             11294..11803
                     /locus_tag="WIB30_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ3796878.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            11966..13348
                     /locus_tag="WIB30_000783"
     CDS             11966..13348
                     /locus_tag="WIB30_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ3796879.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAGIGLAFDGDADRLGLVTKDGNIIYPDRQLMLFAQDVLSRNPGAK
                     VIFDVKSTRLLAPWIKEHGGKAIMEKTGHSFIKSAMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQNISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNQFKAVI
                     ESNPNLSWPL"
     gene            complement(13404..14159)
                     /locus_tag="WIB30_000784"
     CDS             complement(13404..14159)
                     /locus_tag="WIB30_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ3796880.1"
                     /translation="MIKIRNIHKTFGENTILHGIDLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(14169..14885)
                     /locus_tag="WIB30_000785"
     CDS             complement(14169..14885)
                     /locus_tag="WIB30_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ3796881.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(14875..15702)
                     /locus_tag="WIB30_000786"
     CDS             complement(14875..15702)
                     /locus_tag="WIB30_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3796882.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            15987..16895
                     /locus_tag="WIB30_000787"
     CDS             15987..16895
                     /locus_tag="WIB30_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ3796883.1"
                     /translation="MSTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT         4209 a         4900 c         4170 g         3705 t
ORIGIN      
        1 agcattaaaa aatgccgtct gaacgtcctt acgttttcag acggcattgc cttatgccca
       61 aataacggtt tcttccaagg gcctgcgcgc tttcgtggcg atttcctgaa cgcggtagcc
      121 gaatgtcgcg gcaacggaca cgccccattc tgccgcatcg aacaaaccga actgcccgga
      181 caatacgcgt tccatctcgg catagttgaa accttccacc gggcaggaat cgatacctgc
      241 catcgccgca cccgtcatca tgttgcccaa cgcgatatag gtctggcggc aacaccagtc
      301 aaacaaggcg cgcgaatcgt ccaaaatctt gatgtcgtcg gcttgaaacg cctgatacct
      361 cgccaaagat tttgccatgg catccggttc ggtaacgccg cgccgtttga ggctttccaa
      421 cataaacggg ctgtcgaagc gggcattttt ctttgccaaa aacaccacca aatgactggc
      481 ggtatccaaa gcatccgcca taccccaaga aaacggcttg attgcctgtc ggatttcggg
      541 gttttgaacc acaacaaact gccaaggctc cgaaccgacc gaactgggcg acaaacgccc
      601 gagttctaaa ataaactgaa aatcctcggc actgatttta cgtgccgcat cgtaatgccg
      661 gcatgatttg cggtttttaa atgcggatag aacctgctct ttgcttaata ctgccatcgc
      721 acatcctttc attacttgta ttgcaaattg atttcaaacc gatacggcac tgccgcacca
      781 tacaatccgt cctcgccatc aagcttcatc cggactttac ccatctctct acggctggga
      841 aaacaaagcg gcggcagact cgggaataac gccccaatgc ctttcagacg gcatttgggc
      901 atcattgcaa acgcgcctac gggtaggcga tataggcata agccgaatgg ttgtggatag
      961 actcgaaatt ctcgctctcg acaacgaaac tcttgatgcg tttgtcggcg atcagcgaag
     1021 tagcgacatc acgcaccata tcttccacga atttcgggtt ttcgtaggct ttttcggtaa
     1081 catatttttc atcggggcgt ttgagcaaac cgtagagttg gcagcttgcc tgcgcctcca
     1141 cataatcaat gacttcctcg ataccgactt ctgcatcggc agtcaggctg acggtaacat
     1201 gcgaacgctg gttgtgcgcg ccatattggg aaatttcttt ggaacacggg caaagcgagg
     1261 ttacgggaat catgaccttc atactgtagc cgtatgcccc gtctttcatt tcgcccgtga
     1321 gacagacatc ataatccagt aaggaccgaa taccggaaac cggcgcggtt ttcttgcgga
     1381 aaaacggaaa agaaacgctg attttgccgg cgcgggaatc taaaagcgcg accatttcgg
     1441 cagtcagcct gtgcaattgt gcaaaatcca aggcttcaga atgttgctcc atcaatgcga
     1501 caaaacgcga catatgcgtc cccttctgct cagcaggcag gtaaaccgtc atggtcagac
     1561 gggcaacggt ggactggatg ccttctgcag tttgcagggt aatcggaaag cgcaggtctt
     1621 tgataccgac ctgattgatc ggcagattgc gcaaatctcg gctggattgc acgtctgcaa
     1681 tagtgttcat gagttgtttt tccttaatat tcatgtaatt gttgagatgt gtgcatggta
     1741 tggcgtttcc gccggggaat ataccgtcaa tctgcaagtg cgagattata tcactcgctt
     1801 ttagcagctg cattgattgt ttctattttt cagatagtga taaattttca gatagtgata
     1861 aaatcttcga ttattcacat ctacataggc agagaccaaa tgaaattcgc cacgaaagcc
     1921 attcattcca gctacgattg cgacgaacac aaccgcgcgc tgatgccgcc gatttatcaa
     1981 aacagtatgt ttacgttgca cgagattggc gaaaatgtgc cttaccgtta ttcgcgcctg
     2041 agcaacccga cccgtcagat tttggaagac accgttgccg atttggaaca cggtgcggca
     2101 ggctttgcgt tttccagcgg tatggcgggg attgatgccg tatggcgtac cttcctgcaa
     2161 ccgggcgata ccatcgtcgc ggtcgccgat atttacggcg gcgcgtatga cttattggtc
     2221 gatgtttatc aaaaatgggg ggtaaatgtt gtttttgccg atttaggcaa tcccgacaat
     2281 ttggacgaat tgctcaaagc gcacaaggtc aaactggttt ggctggaaac gccgtccaat
     2341 ccgcttttac gcttggtaga catcaaagcc cttgccgcaa aagccaaagc gaccggcgcg
     2401 ctggtcggca tcgacaacac ctttgccacg ccgtatctgc aacagccgtt ggatatgggt
     2461 tgcgattttg tgttccattc cgctaccaaa tatttgtgcg gccattccga cgtattgatg
     2521 ggcatcgtcg ttgccaaaac caaagagctg gctcagcctt tgcacgatat gatggtgcat
     2581 accggtgcga ttgcaggccc aatggactgc tggctggtgt tgcgcggcat caaaacgctt
     2641 gccctgcgta tggaagcaca ctgcaaaaac gcactcgaaa tcgcgcgccg tctggaagac
     2701 catcctgcca ttgaaaaagt gttccatccc ggcctgccgt cgcacgaaca ttatgaactc
     2761 gcccaagcgc aaatgcctaa aggcatcggc ggcgtggtga cggtttatct caaaaacgac
     2821 acgcgcgaag cggcaaacag cgtgattaaa aacatgaaac tggtcaaaat ggcttccagc
     2881 ctcggcggcg tggaaagttt ggtcaaccat tgctattccc agtcccacag cggcgtgccg
     2941 cataatgtga aaatggaaat gggcatcaaa gtcggtctgc tgcgtttctc catcggcatc
     3001 gaagacgcgg acgatatttg gaacgatatt tccgccgcac tcgatacaac tttgtaaact
     3061 gtaaaaatgc cgtctgaaac catggtttca gacggcattt caattaaccc ggccgaaaat
     3121 caacgcttca acatctttgc cgcctcaata gcgtaatagg tcaaaatccc gtccgcaccc
     3181 gcacgtttga atgccagcag gctttccaaa accactttgc cgccgtccag ccagccgttg
     3241 gcaatcgctg cctgcaacat cgcgtattct cccgaaacct gataggcata agtcggtacg
     3301 ccgaactcgt ccttcacgcg gcggacaacg tccaaatacg gtaaaccggg cttcaccatc
     3361 accatatccg caccttcctg aatgtccaac gccacttcat tcagcgcctc atcggtattt
     3421 gccggatcca tctggtaggt tttcttgtcc gccttgccca aattgcccga actgcccacc
     3481 gcatcgcgga acggcccgta aaacgccgaa gcatatttgg cggaatacgc cataatccgc
     3541 gtgtggatat gcccggcatc ctccaacgcc tcgcgaatcg caccgatacg cccgtccatc
     3601 atatcggaag gggcaaccac ctgcgcgccc gcttcagcgt ggcacaaagc ctgcttgacc
     3661 aaaacctcta cggtttcatc gttcatcaca taaccgtttt cgtccgtcag cccgtcctga
     3721 ccgtgaaccg tataaggatc gagcgcgaca tccgtcataa tgcccagttc gggaaacctc
     3781 tcgcgcaggg cgcggacggc tgccggcacg agtccttcgg gattgtacgc ttcctctgca
     3841 cgctcggttt tgtttgccgt aaccacggga aagagtgcca acatcggaat accgagcttt
     3901 accgcctctt ccgccgtaaa cagcagcctg tccaaacttt gacgcttcac acccggcata
     3961 gaaggcacat cctcctcgcg cgccgacccc tccaatacga acaccggata aatcaaatca
     4021 tcggcggtca gcgtgtgttc gcgcatcagg cggcgtgaaa aatcgtccct gcgcatacgg
     4081 cgcatacgcg aagccggaac attgcggtaa ggaaactgca taagccctcc aatcatcctg
     4141 tcaacaattc aaaccatacg gaagccgccg caacttgcag acagcatcgg gcggcaatca
     4201 aaatccgttt tcaccggaaa gggtttcggc ccgccgaaat gccgcgccga aggacggcgt
     4261 cccgcccatt aaagaacaat agcaaaacaa tagcttcagg ctgtcagcct tcgatattct
     4321 tacgccacaa taccaaaagt ttcccgatat gctgaacagg ttgcgcgtca accgcctcac
     4381 ataaggcagt gcagatttcg atacgttcgg cacggtcgtc gccgaatacg cgcactttaa
     4441 tcagctcatg cgccgtcaat gccgcatcgg tttccttgat gaccgcgtcc gtcagaccct
     4501 gctgaccgac catcacaaca ggatggagat ggtgcgcgcg cgctttcagt tccaaaattt
     4561 ctttggtgtt caatttggta tccgtcattt tcctactgct tgaataaaaa gaataatgcg
     4621 gcattgtacg cgatttggca cgacatcggc aaaattaacg tacaatacgc ggtttccatt
     4681 ccgacccgcg aaccactatg gctgtacgtt ccaaatcctc aaaagcgtgg ctgcacgaac
     4741 acgtcaacga ccactacgtc catatggcgc aaaaagacgg ctaccgcgcc cgtgccgcat
     4801 acaaactttt ggaaatcaac gacaaagaca aattaatcaa acccggcacg gtacttgccg
     4861 acttgggcag cgcgccggga agctggtcgc aggttgccgc caagctggcc ggcccttccg
     4921 gcgcggtatt cgctttggac atcctaccca tggaagccat agggggcgtc tccttcattc
     4981 agggcgactt ccgcgagaac gacgtactgg cacaatttga aaccttgttg gacaaccgcc
     5041 cgctcgacct tgtaatttgc gatatggcgc ccaatatgtc gggaaacgcc gtaagcgatc
     5101 aggcacgcag cctctatctg tgcgaattgg cccttgactt tgcctcgcaa cacctgaaaa
     5161 caggcggcag ctttttagtc aaagtctttc agggagcagg ctatcaggaa tacatggcag
     5221 ccatgcgcga aattttcggc acggtgcaga cgcgcaaacc cgaagcctca cgcaatcgct
     5281 ccagtgagat ttatttattg ggcaaaaata aacgctgaca atacagacgg cgtgctttac
     5341 aatcatttcc gttttacacc tcaattatgg agccttgcta agtggggaac acctttaaat
     5401 caatccttgt ctgggtcgcc ttgggtatcg gcctgatggc tgcgttcaac gctttagacg
     5461 gtaaaaaaga agacaacggg caaatcgaat actctcagtt catccaacag gtcaacaacg
     5521 gcgaagtatc cggcgtcaac atcgaaggat ccgtcgtcag cggctacctg attaagggcg
     5581 agcgcaccga caaaagcact ttcttcacca acgcgccttt ggacgacaac ctaattaaaa
     5641 cactgctcga caaaaacgtc cgcgtaaaag taacgccgga agaaaaaccg agcgcgctgg
     5701 ctgccctgtt ttacagcctg ctgcccgtcc tgctgctgat tggcgcatgg ttctacttca
     5761 tgcgtatgca gacgggcggc ggcggaaaag gcggcgcatt ctcattcggt aaaagccgcg
     5821 cccgcctgct ggacaaagat gccaacaaag tgacctttgc cgatgtcgcc ggctgcgacg
     5881 aagccaaaga agaagtacag gaaatcgtcg attacctcaa agcgccgaac cgctatcaaa
     5941 gcctgggcgg gcgcgtgccg cgcggcatcc tgctggcggg cagcccgggt acgggtaaga
     6001 cgcttttggc gaaagcgatt gcaggcgaag ccggcgtgcc gttcttcagc atttcaggtt
     6061 ccgactttgt cgaaatgttc gtcggtgtcg gtgcgagccg cgtccgcgat atgttcgagc
     6121 aggcgaagaa aaacgccccc tgcatcatct ttatcgacga gattgacgca gtcggccgcc
     6181 aacgcggcgc aggtttgggc ggcggcaatg atgagcgcga gcaaacatta aaccaattgt
     6241 tggttgaaat ggacggtttt gagagcaatc agactgtaat tgtgattgcg gcaaccaacc
     6301 gccccgacgt actcgatcct gcgctgcaac gccccggccg tttcgaccgc caagtggttg
     6361 tccccctgcc ggacatccga gggcgcgaac agattttgaa cgtccattct aaaaaagtgc
     6421 ctttggacga atctgtggat ttattgtccc tcgcgcgcgg cacgccgggt ttttccggcg
     6481 cggatttggc gaacttggtc aacgaagccg ccctgtttgc cggccgccgc aataaagtca
     6541 aagtcgatca gagcgatttt gaagacgcca aagacaaaat ctatatgggt ccggaacgcc
     6601 gcagtatggt gatgcacgaa gacgaaaaac gtgcgacggc gtatcacgaa tccggacacg
     6661 cgattgttgc cgaaagcctg ccctttaccg accccgtcca caaagtaacc attatgccgc
     6721 gcggacgtgc gctgggtctg acttggcagc ttccggagcg cgaccgcatc agtatgtata
     6781 aagatcagat gttgagccag ctctccatcc tgttcggcgg acggattgcc gaagacatct
     6841 tcgtcggacg catctccacc ggcgcatcaa acgactttga acgcgcaacc caaatggcgc
     6901 gcgaaatggt aacgcgctac ggcatgagcg acaaaatggg cgtgatggtt tatgcggaaa
     6961 acgaaggcga agtcttcttg ggacgcagcg taacccgttc gcaaaacatt tccgagaaaa
     7021 cccagcagga catcgacgcg gaaatccgcc ggattttgga cgagcaatat caggttgcct
     7081 acaaaatcct cgatgaaaac cgcgacaaga tggaaacgat gtgcaaagcc ctgatggaat
     7141 gggaaaccat cgaccgcgat caggtactgg aaatcatggc gggcaaacaa cccagcccgc
     7201 ccaaggatta cagccacaac ctgcgcgaga atgcggacac ggcggaagat aacgcgccgc
     7261 acgccccggc tcgggaagaa accgaagcac ctgctccggc agacaccgct tcgacagagt
     7321 ccgagcagca gcctgaaaac aaggcttaac ttcccgaaca aacggcagcc cgcaagctgc
     7381 cgttttccca tcctgttttc agacggcatt ttccgctcaa tgccgtctga aacgccaaag
     7441 ccgtatcggt ttttgcgaat ctgcgatatg gcggtttaaa ttgaaatcag acgaggcaag
     7501 gctgtaccgg tttaaaatta aaccgctata atactgccct tcgaatacac gcccgcattg
     7561 gtgatgcccg tgaaaaaagt tgaaaaaaac atcttggtgc tgcacggcgc ggacaaaatg
     7621 ttcgagctgg tcgataaggt tgaggattac ccgcactttc tgccgtggta cagcaaaacc
     7681 gaggtcatcg ggcgtagcgg caacgaactg aaggcacggc tgtttatgga ctatatgcac
     7741 gttcgccaat cattcgccac gcacaaccgc aacattccgg gcagggaaat ccgtatggaa
     7801 ctgctcgagg gtccgttcaa aaccttacgt ggaacgtgga aatttatcga tttgggcgac
     7861 gatatgtgca aaatcgaatt caatttggaa tacgattttt ccaatgccgt tttgtccgcc
     7921 ttaatttccc ccgtcttcaa ccacctttcc gccacgctgg tcgaagcgtt cgtcaaagag
     7981 gcagaccgcc gttatgcttg aaattgagat tgtgtacggg ctgtctgata agcaggtttt
     8041 gaaaaatatg cagcttgccg agggaacaac cgtccgcgcc gccgcactgc aaagcggttt
     8101 ggacggcata tttgaagatt taaacctgca ttccgcgcct ttgggcattt tcggcaaagc
     8161 cgtcaaagac gacacgcccc tgcgcgacgg cgaccgcatc gaagtgtacc gcccgctgtt
     8221 gatcgacccc aaagaagcgc gccgcaaacg cgttcaaaat caagaagaat aaccatgccg
     8281 tctgaagcct tcagacagca ttcgacagaa acacggaaaa tatcatgtca aacaaaatca
     8341 aaatgcttgt cggattgggc aaccccggca aagaatacga acaaacccga cacaatgcag
     8401 gcttttggtt cctcgacgaa ctggcttgga agtggaaggc ttcatttaaa gaagaaaaaa
     8461 aattcttcgg cgaagtcgcc cgtgccgccc tgcccgacgg cgatgtctgg ctgctcaagc
     8521 cgaccacgtt catgaaccgt tccggacagg cggttgccgc ccttgcgcag ttctacaaaa
     8581 tcaaacccga agaaatcctc gtcgtccacg acgaactcga cattccctgc ggacggatta
     8641 aattcaaact cggcggcggc aacggcggac acaacggctt gaaagacatt caggcaaaac
     8701 tcggcacggc agactattac cgcctgcgcc tcggtatcga ccacccgggc gaccgcaacc
     8761 tcgtcgtcgg ctatgtcctg aacaaaccca gtacggaaca ccgccgacag attgacgatg
     8821 ccgtcgccaa atccctgcaa gccatacccg acatccttgc cggcaaatgg gaagaagcaa
     8881 cccgcttcct gcacagcaaa tgacccgatg ccgtctgaag ccctttcaga cggcatgttc
     8941 ccgatttcca tatccgaaca gtcatgaccg aactcaagca gcttatccaa accgaatcca
     9001 tccccgtcat cgaagaaacc ctcgatttcc tgctgtacga atgcagcatc gacgacgcac
     9061 catccgccga agaagtggca caatggcgcg acatacttgc cgcacgcggc ggcaaattcc
     9121 tgcgcctgtc caaaatctgc caaacgtggc tggacgagga ggcggcatga agctgccgcg
     9181 caaccgcttc agcctgcttt ccgcattgtg gtttgccggc ggcatctatt cgctgctctt
     9241 caaagctgcc gacaccgcgc cgccgccgtt tccgcatttc gacaaagcag cacaccttgc
     9301 cctgtttttc gcacaaatct ggcttttgac caaagcattc aaaaccggaa aacttcccat
     9361 cccctaccgc agcctgatgg tctttgccct ctgtttcgcc ctcttcagcg aatgcgcgca
     9421 ggcatggttt accgcaacga gaaccggcag tttgggcgat gttcttgccg atatggcagg
     9481 tacggttctc gcactctttg ccgcccgcgc cgccgaccgc ccggactgaa tcggtttatt
     9541 tcccccaaac aggatgcacc tcttcccgat acatcctatt tttcccctta catatcaatc
     9601 ccatctcata aacaaaaccg acaaatcgtt tacaatatat ttacactaca tcagattaca
     9661 aatatactcg acccagttgc agaatcggcg gtttcacaaa ccgtttcgga ttgcacgaca
     9721 caaaagaaaa accgatttta ttgccttaaa ggagtattca tgaacctgca tgcaaaggac
     9781 aaaacccagc atcccgaaaa cgtcgagctg ctcagtgcgc agaagccgat taccgacttt
     9841 aagggtctgc tgaccaccat tatttccgcc gtcgtctgtt tcggcattta ccacatcctg
     9901 ccttacagcc cggatgccaa taaaggtatc gcgctgctga ttttcgttgc cgcactttgg
     9961 tttaccgaag ccgtccacat taccgtaacc gcactgatgg tgccgattct cgccgtcgta
    10021 ctcggtttcc ccgacatgga catcaaaaag gcgatggctg atttttccaa cccgattatc
    10081 tacatttttt tcggcggctt cgcgcttgcc accgccctgc atatgcagcg gctggaccgt
    10141 aaaatcgccg tcagcctgtt gcgcctgtcg cgtggcaata tgaaagtggc ggttttgatg
    10201 ttgttcctcg ttaccgcctt tctgtccatg tggatcagca acaccgccac cgccgcgatg
    10261 atgctgcctc tagcaatggg tatgctgagc cacctcgacc aggaaaaaga acacaaaacc
    10321 tacgtcttcc tcctgctcgg catcgcctat tgcgccagca tcggcggctt gggcacgctc
    10381 gtcggctcgc cgcccaacct gattgccgcc aaagccctaa atctggactt cgtcggctgg
    10441 atgaagctcg gcctgccgat gatgctgttg attctgccct tgatgctgct ctccctgtac
    10501 gtcatcctca aacctaattt gaacgaacgc gtggaaatca aagccgaatc catcccttgg
    10561 acgctgcacc gcgtgatcgc gctgttgatt ttccttgcca cagccgccgc gtggatattc
    10621 agctccaaaa tcaaaaccgc cttcggcatt tccaatcccg acaccgttat cgccctgagt
    10681 gccgccgtcg ccgtcgtcgt cttcggcgtg gcgcaatgga aggaagtcgc ccgcaatacc
    10741 gactggggcg tgttgatgct cttcggcggc ggcatcagcc tgagcacgct gttgaaaaca
    10801 tccggcgcgt ccgaagcctt gggacagcag gttgccgcca ccttttccgg cgcgcccgca
    10861 tttttggtga tactcatcgt cgccgccttc attatttttc tgaccgagtt caccagcaac
    10921 accgcctccg ccgcattgct tgtaccgatt ttctccggca tcgctatgca gatggggctg
    10981 cccgaacaag tcttggtatt cgtcatcggc atcggcgcat cttgtgcctt catgctgccg
    11041 gttgccacac cgcctaacgc gattgtgttc ggcacgggct taatcaagca acgcgaaatg
    11101 atgaatgtcg gcatactgct gaacatcctc tgcgtagtat tggttgctct gtgggcttat
    11161 gctgtactga tgtaaaccat cgacctaaac aacaagaccg tctgaaagaa tatttttcag
    11221 acgaccttga agtttgtcgt tcagacacaa tttgtcgaat cattcaaaac cggattctaa
    11281 cgaaaggaaa cccatgatta tcctgcacac caacaaaggc gacatcaaaa tcgaactcga
    11341 tttcgacaaa gcccccgtta ccgccaaaaa cttcgagcaa tacgtcaaag acggcttcta
    11401 cgacggcgta atcttccacc gcgtcatcaa aggcttcatg attcaaggcg gcggcatgga
    11461 tgaaaacatg aacgaaaaag aaacccgcga tccgattcaa aacgaagcgt ccaacggcct
    11521 gcccaacgat aaatacacca tcgccatggc acgcacttcc gacccccatt ccgccagcgc
    11581 gcaattcttc atcaacactg ccgacaacgc tttcctgaac ttccgttcta aagagctgta
    11641 cggcaaaacc gtcgtccaag actggggcta tgcagtattc ggtaaagtcg ttgacggttt
    11701 tgacgttgtc gatgccatcg aaggcgtatc caccaaacgc cacggctacc atgacgacgt
    11761 accgagcgaa cctgtcgtca tcattaaagc cgaagcggta taaaccggca atccgaaagc
    11821 agcctgcaca aaggctgctt tttttgtttc aggcggcata aaatccaagc acatatgccg
    11881 tctgaacacc tcccgcaagc cttcggacaa tgttgcaacg tgttaaaata gcctcctttc
    11941 ctgaaaccac aacaaggaaa ccatcatggc aaatattgcc cgcgacatct tcaaagccta
    12001 cgacatccga ggcattgtcg gcaaaaccct gactgacgat gccgcctatc tcatcggcag
    12061 ggccatcgcc gccaaagccg ccgaaaaagg catgacccgc atcgcgctcg gacgcgacgg
    12121 acgcttgagc ggtcccgaac tgatggagca catccaacgc ggctttaccg acagcggcat
    12181 cgacgtcctc aacgtcggta tggtcgccac gcccatgctc tattttgccg ctatcaacga
    12241 atgcggcggc agcggcgtga tgattaccgg cagccacaat ccgcccgatt acaatggttt
    12301 caaaatgatg ctcggcggcg acacgctcgc aggcgaagcc attcaagaac ttttagccat
    12361 tattgaaaaa gacggttttg ttgccgccga caaacaaggc aacgtaaccg aaaaagacat
    12421 ctccggcgaa taccacaacc acatcgtcgg ccacatcaaa ctcaaacgcc ccatgaaaat
    12481 cgccatcgac gcaggcaacg gcgtaggcgg cgcattcgca ggcaaactct acaaaggctt
    12541 gggcaatgaa gttaccgaac ttttctgcga cgtggacggc aatttcccca accaccatcc
    12601 cgacccatcc aaacccaaaa acctgcaaga tttgattgcc gcgctgaaaa acggcgatgc
    12661 cggaatcggc ctggcgtttg acggcgatgc cgaccgcttg ggcctggtta ccaaagacgg
    12721 caacattatt tatcccgacc gccaactgat gctgttcgcg caagacgtgt taagccgcaa
    12781 tcccggcgcg aaagtcatct tcgacgtgaa atccacccgt ctgcttgctc cttggattaa
    12841 agaacacggc ggcaaagcca taatggaaaa aaccggccac agctttatca aatccgccat
    12901 gaaaaaaacc ggcgcgctgg ttgccggcga aatgagcgga cacatcttct tcaaagaacg
    12961 ctggttcggc ttcgacgacg gtctgtacgc cggcgcacgc ctcttggaaa tcctgtccgc
    13021 ctccgataat ccgtccgaag tgttgaacaa cctgccgcaa aacatttcca cgcccgaact
    13081 caacatcgcc ctgcccgaag gcagcaacgg ccatcaggtt atcgacgaac tcgccgccaa
    13141 agccgaattc gaaggcgcaa ccgaaatcat caccatcgac ggtctgcgcg ttgaatttcc
    13201 cgacggcttc ggtctgatgc gtgcttccaa taccacgccg attctggtgt tgcgttttga
    13261 ggcggatacg caagcagcca tcgagcggat tcaaaaccaa ttcaaagccg tcatcgaaag
    13321 caatccaaat ttaagctggc cgctgtaaac atcgaaaaaa tgccgtctga aggcttcaga
    13381 cggcattttt cacaaatagt taatcatatc ttggtagatt ggatttggct taaaaatctc
    13441 cgcgtccgtt cgtgtttggg gtgatcgaac aactctttcg ggctgccttg ttcgacaata
    13501 acgccgccgt ccatcacgac aacggtagtc gccacttcca gcgcaaactt gatttcgtgg
    13561 gtaacgacga ccatcgtcca accttcccgc gccaattcct tcatggcgtt caacacgtct
    13621 tgcaccaact cggggtcaag cgcggaagtg ggttcgtcaa acaacatcag ctcgggctga
    13681 atcgccaatg ctcgggcaat gccgacgcgc tgctgctgac cgccggaaag ctggtaggga
    13741 tacaaatcca ctttgtcgcc caagccgact ttttccagca gtttcagagc ctcttcgcgc
    13801 gcttgggcgg caggcttgcc ctgtacggca accggtcctt ccatcacgtt ttccaaggcg
    13861 gttttgtgcg gaaagaggtt gtattgttga aacaccatgc ctgatttgcg gcgcagtgcc
    13921 aaaatatcgt gtttgcttgg ttttttagaa aaatcgattt tcagcggtcg ctcgttgtcg
    13981 aactcgattt gtccgtcttc gggcatttcc aacgcgttta ggcagcgcag aaacgtcgtt
    14041 ttgcctgagc cggaaggccc gaggatgacg accacctgcc ctttgcacac atccaaatcg
    14101 atgccgtgca aaatggtatt ttcgccgaag gtcttatgga tattgcggat tttaatcatg
    14161 acaactcctt atttggcgac gtagcggtcg aaacgttttt ccaaacgcgc ctgaatcagg
    14221 aacagcactt tacaaaaaca ccagtaaacc aaagcggctt cgatatagac gggcaaaaag
    14281 tcataagtgc ggtttgccgt ttcctgcgcg acgcggaata attccgttac cgtcacgact
    14341 gccgcgagcg aggtgttttt aaacaaaccg ataaactcgt tgctcaaagg cggcacggca
    14401 acgcggaatg cctgcggcgc gacaatgcgg cggaacgtct gcatataggt catgccgatg
    14461 gagaaacctg cttcccattg gcctttcggt acggacaaaa ttgccgcgcg tatggtttcg
    14521 gaagcatatg cgccgacatt gagcgaaaag ccgatgatgg cggcaggaat cgggtcaata
    14581 tagatgccga cggaaggcag cccgtaaaac acaatcacaa gctgaaccaa cagcggcgta
    14641 ccgcgaatga cggaaatata aaattccacc aatttcagca ggattttccg cacgatgccg
    14701 ccggcgggca taatccgcac caaagccacg gctaccgcaa tcatcatacc gataacgaaa
    14761 gaagctgccg ccaaaggcag agagaccgcg aagccggctt tgaccatagg caaaaacgcg
    14821 ctgacaatca tatcggcgcg tgtttccgtc ataaacggca gcgaagcaag gaaattattg
    14881 aacactgatg tcttttccga agaattgttc gcccagtttt ttcagcgtac cgtcggcttt
    14941 cagctcgttg attgccgtac tgaatttcgc cacggcttcg tcattgccct tgttgacaat
    15001 caggccggaa ccgacttttt catcggcagg tgcggaccaa acgattttca cgcccgcatt
    15061 cgggtttttc ttcagatagt ccaaaaccgc caattcgtcg ttcagggttg catcggcacg
    15121 tttttgttca atcagggtca gcgattgcgc caaaccgtca acagccacca aatctgcgcc
    15181 tgcagctttg gctttttcgc cgtagttgct ggtcagggat tgtgcggttt tcacgccttt
    15241 gatgtcgtcg atagatttga tgttgctgtc gttacgggca accaatacgg caccgctcca
    15301 gctgtaagga tcggatttgt cgaatgtcgc ttggcgttcg gggctggtca gaccgacttg
    15361 gtttgccacc acgtcgaaac gccccgcctt caaacccgcc atcatcgaat cccattgcgt
    15421 ttctttaaac tcgactttca cgcccagttt ttccgccacg gcgcgggtta cttccacatc
    15481 gtaaccggtc agtttgccgt ccttgtcgtg gtaggtaaac ggtgcgtaag tgccttccgt
    15541 gccgacggta accgtgcctt tattgttgat gcgctcgatt aaagaaccgg aaactgccga
    15601 ttgtgcaggc gcggaagatg ctccgctgcc gccttccgaa ccgccgcagg ccgccaaaac
    15661 caatgcggca atgccgccga gtacgaattt tttcaacatc atcctctcct gtaaaaagtg
    15721 tgcaagtgcg gcattctaag tgaaattgcg gcaaacacaa agaatccttt ttttatatgg
    15781 ttattccaaa tcggaatgga aaagatatgc cgaagccgcc ggaagcggaa ataaaggggg
    15841 cgggaaaggc ggcgggcggc acgaaaccga aaagcggcat caggtatgca aacggcggat
    15901 tcacgcacgc cgttccggca aaaatccgcc tgccgattcg gtgctataat ccatccgaaa
    15961 cagatgacac caaaaaggaa catctcatga gcacgcccca atccgccatc attcccgacc
    16021 acgcccaagc cggcatcttt atcgaagccg acttcgccgc caaccgcctc aacgatatta
    16081 aagccgcctg ccgcgcttcg ctcgacgcgt taactgcctt gaaagcccgc tttccggacg
    16141 atattttggg tctgaccatc gccttcggca gcaaagcctg ggcaacattc ggacatacgg
    16201 acgaaggcag tgaaatcaaa cccttctccg aaatgggcaa cgggcttgcg ccgtccacgc
    16261 agcacgatat gtccatccac atccaatcct tccgccaaaa cgccgcctac gcgcttgccc
    16321 aatccgtttt gggcgcgttc ggcgacagca tatgcgtcgc gtccgaagaa cacggtttgc
    16381 gcctgtatca ggatcgcggg ctggacggtt tcgtcgacgg cacggaaaac ccgcagggcg
    16441 atgaaaatgt ccgcgaagtc gccatcatcc ccgaaggaaa acccgatgcg ggcggcagct
    16501 atgtcctgct gcaaaaatac ctgcacgatt tgaaaaaatg ggatgccgtc cccgtcgccg
    16561 aacaggaggc ctcggtcgga cgcggcaagg aaaccgacga cgaattcggc cgcgatgtcc
    16621 gcctgcccga ttcgcacctc ggccgcgtca acctgaaaga aaacggagtc ggcctgaaaa
    16681 tcgtccgccg cagcctgccc ttcggcaaaa tcagcggcga acacggtttg atgtttaccg
    16741 catactgccg cacgctgcac aatatcgaag cgcaattatt gagtatgttc ggcgatacgg
    16801 acggcaaaac cgacctgctg ctccgacatc tctccgccgc cgtttcgggc ggatactact
    16861 acgcgccgtc tgtcgaacgc ctgcaaaacc tctaagccgg acgatgccgt ctgaaaccat
    16921 ttttcagacg gcattttccc gttttatagt ggattaacaa aaaccagtac ggcgttgcct
    16981 cgcc
//
LOCUS       DAWHRA010000032        16613 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWHRA010000032 DAWHRA010000000
VERSION     DAWHRA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.032"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            397..5844
                     /gene="iga"
                     /locus_tag="WIB30_000788"
     CDS             397..5844
                     /gene="iga"
                     /locus_tag="WIB30_000788"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050177927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ3796884.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGANN
                     DITWLGAGIDVADSKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTSHASTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRRP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRISSFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGANLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSAQKDAHFSKNNEVVFEDDWINRTFKATEITVNQSASFSSGRNVS
                     NITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANQYHTLKINHLSGNGHFHYLTHLAENLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVRDRSHLSVSLANHHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQ
                     QAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQK
                     VEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQS
                     QPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELL
                     AKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQAL
                     AARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAEL
                     ARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQDY
                     MAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDEN
                     VSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHE
                     KVETVSLQPRAAQPRAQAATQPQAQAAAQADAVSTNTNSALSDAMASTQSILLDTGAS
                     LTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQI
                     GGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQKA
                     NFNRASIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLT
                     AGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQYSAGAALLY
                     RNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            5912..6022
                     /locus_tag="WIB30_000789"
                     /pseudo
     CDS             5912..6022
                     /locus_tag="WIB30_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6024..7226)
                     /gene="trpB"
                     /locus_tag="WIB30_000790"
     CDS             complement(6024..7226)
                     /gene="trpB"
                     /locus_tag="WIB30_000790"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ3796885.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(7309..7998)
                     /locus_tag="WIB30_000791"
     CDS             complement(7309..7998)
                     /locus_tag="WIB30_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ3796886.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            8195..8974
                     /gene="rsmA"
                     /locus_tag="WIB30_000792"
     CDS             8195..8974
                     /gene="rsmA"
                     /locus_tag="WIB30_000792"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ3796887.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRNIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            9147..9875
                     /locus_tag="WIB30_000793"
     CDS             9147..9875
                     /locus_tag="WIB30_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ3796888.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVLFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            9948..10307
                     /locus_tag="WIB30_000794"
     CDS             9948..10307
                     /locus_tag="WIB30_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796889.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            10312..10752
                     /locus_tag="WIB30_000795"
     CDS             10312..10752
                     /locus_tag="WIB30_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ3796890.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGWMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGTTVSQCIIPDMTDKKSYRRLKVWILWRQPKKTAETDTPS"
     gene            10784..12058
                     /gene="folC"
                     /locus_tag="WIB30_000796"
     CDS             10784..12058
                     /gene="folC"
                     /locus_tag="WIB30_000796"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ3796891.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARSEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPESLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACTLTVLECLNAKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            12071..13069
                     /gene="ftsN"
                     /locus_tag="WIB30_000797"
     CDS             12071..13069
                     /gene="ftsN"
                     /locus_tag="WIB30_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ3796892.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEYSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAVGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            13062..13559
                     /locus_tag="WIB30_000798"
     CDS             13062..13559
                     /locus_tag="WIB30_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ3796893.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLVMLASKTDLPDTEEWQQSYTIPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            13767..15311
                     /gene="purF"
                     /locus_tag="WIB30_000799"
     CDS             13767..15311
                     /gene="purF"
                     /locus_tag="WIB30_000799"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ3796894.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTIIARQCSDKAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            15411..15902
                     /gene="greB"
                     /locus_tag="WIB30_000800"
     CDS             15411..15902
                     /gene="greB"
                     /locus_tag="WIB30_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ3796895.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            15961..16587
                     /locus_tag="WIB30_000801"
     CDS             15961..16587
                     /locus_tag="WIB30_000801"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ3796896.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFHRPYIK
                     AIRVQTTSDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDAAKVAAFIATANRLFR"
BASE COUNT         4666 a         4504 c         4095 g         3348 t
ORIGIN      
        1 acggaaacct gcaccgcgtc attcccacga acccccatct cgtcattacc acgaaagcgg
       61 gaatctagga cgcagggtta agaaaaccta catcccgtca ttccctcaaa aacagaaaac
      121 caaaatcaga aacctaaaat cccgtcattc ccgcacaagc gggaatccaa aaacaaaaag
      181 ccgcagaaat ttaacggaag aaccgaaccc cgaaaaccgg cttcccgcct tcgcttgaat
      241 aacagaacaa aggaagcgga aataaaaaca tcgggattct ttttttaaat tggcaataaa
      301 acaccaaaat gaatgattta cacaacaaaa attagtcaac accgccaaac cggcgtaaaa
      361 tacaaaacat tatcaccctt aaaacggtaa aaccttatga aaaccaaacg ttttaaaatt
      421 aacgccatat ccttatccat ctttcttgcc tatgccctta cgccatactc agaagcagca
      481 ttggtcagag acgatgtcga ttatcaaata ttccgtgact ttgcagaaaa caaaggcaaa
      541 ttttttgtcg gcgcaaccga tttatcagtg aaaaacaaac aaggtcaaaa catcggcaac
      601 gcattatcca acgtaccgat gattgatttt agcgttgcag atgtcaacag acgtacatta
      661 accgtcatcg atccccagta tgccgtcagc gtcaaacatg taaaaggaga cgaaatctct
      721 tattacgggc atcacaatgg gcacttagat gttagcaatg atgaaaacga atatcgttca
      781 gttgcacaaa atgactacga accaaataaa aattggcatc acggtaatca aggtcgtctt
      841 gaagactaca acatggcacg ccttaataaa ttcgtaacag aagtcgcacc tattgcacca
      901 accagtgcag gcggcggcgt tgaaacctat aaagataaaa accgtttttc tgagtttgtg
      961 cgagttggag caggtacgca atttgaatat aacagccgct acaatatgac agagctttca
     1021 cgagcttatc gttatgccat tgcaggtacg ccttatcaag acgtgaatgt cacatcgaat
     1081 ctaaatcaag aaggcttgat tggcttcggt gataattcaa aacatcattc cccagaaaaa
     1141 ctcaaagaag ttttatctca aaacgcattg actaactatg ctgtgttagg cgatagcggt
     1201 tcaccattat ttgcttatga taaacaagaa aaacgctggg tctttttagg tgcttacgat
     1261 tattgggcag gctatcaaaa aaactcttgg caagaatgga atatctataa aaaagaattt
     1321 gcagataaaa tcaaacaacg cgataacgcc ggcaccatca aaggttatgg cgaacatcat
     1381 tggaaaacca cgggcacaaa cagccatatc ggttcgacag cggtaaggct tgccggcaat
     1441 gaaaggggtg caaacaacgg acagaatgtt acctttgaaa acaacggcac tttggtattg
     1501 gatcaaaaca tcaaccaagg cgcgggcggt ctgtttttca aaggcgatta cacagtcaaa
     1561 ggtgcaaata atgacatcac ttggctaggt gcggggattg atgttgccga cagcaaaaaa
     1621 gtcgtttggc aagtcaaaaa tccgaatggc gacagattgg caaaaatcgg caaaggcaca
     1681 ttagaaataa acggcacagg cgttaaccaa gggcaattaa aagtcggcga cggtacggtt
     1741 attctgaatc aacaagccga tgccgacaaa aaagtccagg ctttctccca agtcggcatt
     1801 gtgagcggac gcggtacatt ggtattaaat agttcaaatc agattaatcc cgataactta
     1861 tatttcggtt tccgtggcgg tcgtttggat gccaatggca atgacttgac ttttgaacac
     1921 atccgcaacg tggatgaagg cgcgcgcatt gtcaaccaca acacaagcca cgcctccaca
     1981 atcacgctaa cgggtaaatc tttaattacc aatccgaaca gcctttcggt acatagcata
     2041 caaaatgact atgacgaaga tgattatagt tattactacc gaccaaggcg cccgattccg
     2101 caaggcaaag acctttatta caaaaactat cgttattacg ccctaaaatc cggcggcagc
     2161 gtgaacgcac cgatgcccga aaacggacaa acggaaaata acgactggat tttaatgggc
     2221 agcacacaag aagaagccaa gaaaaacgcg atgaaccaca aaaacaatca gcgtattagc
     2281 agttttagcg gtttctttgg cgaagaaaac ggaaaaggac ataacggcgc attaaacctt
     2341 aatttcaatg gcaaaagcgc gcaaaaccgt ttcttgttaa caggcggtgc caatttaaac
     2401 ggaaaaataa gcgtaaccca aggcaatgtc ttgttatcag gtcgtccaac accacacgca
     2461 agagattttg tgaacaaatc ttcagcccaa aaagacgcac atttctccaa aaacaatgaa
     2521 gtcgtatttg aagacgactg gataaaccgc acattcaaag ccacagaaat tacggttaat
     2581 caatccgcat cattctcttc cggcagaaat gtatccaaca ttaccgccaa catcaccgca
     2641 acagacaacg ccaaagtaaa tttgggttac aaaaacggcg atgaggtttg cgtgcgctcg
     2701 gactataccg gttatgttac ctgcaacaca ggcaacttat ccgataaggc tttaaacagc
     2761 tttggagcga cacagattaa cggcaatgtg aatctgaacc agaatgcggc attggtcttg
     2821 ggcaaggctg cattatgggg gcaaattcaa gggcaaggaa acagccgtgt cagcctaaac
     2881 caacatagca aatggcattt gactggcgac agccaagtac acaatctgtc attggcggat
     2941 agccatattc atttgaacaa tgcctccgat gcgcaaagtg caaatcaata ccacacgctc
     3001 aaaatcaatc atttatccgg taacgggcat tttcattatc tgacgcactt ggcggaaaat
     3061 cttggggata aagtgcttgt gaaggaatcc gcatccggtc attatcagct ccatgttcaa
     3121 gataaaacag gcgaacccaa tcaggaaggg ctgaatctct ttgatgcatc atccgtacga
     3181 gaccgttctc acctttccgt ttccttggcg aatcatcacg tcgatttagg cgcattgcgt
     3241 tatacaatca aaacagaaaa tggcattacc cgattgtaca atccttatgc cgagaaccgc
     3301 cgccgagtca aaccggcccc gtctcctgcc acaaacacgg cttctcaagc acaaaaggca
     3361 acacaaacgg acggtgcaca aattgccaag cctcaaaata tcgtcgtcgc accgcctagc
     3421 ccgcaggcaa atcaagccga agaagcaaaa cgccagcaag caaaagcgga gcaagtgaag
     3481 cgtcagcaag cagaagcaga gcgtaaatcg gcagagttag caaaacaaaa agcagaagcc
     3541 gaacgcgaag caagagagct ggcgacccgc caaaaagcag aacaagaacg cagcagcgcc
     3601 gaacttgcac gtcgacatga aaaagaacgc gaagcggcag agttgtcggc aaaacaaaaa
     3661 gtcgaagccg aacgtgaggc tcaagcattg gcggtacgcc gaaaagcaga agccgaagag
     3721 gcaaaacgcc aagctgcaga gcttgcacgc cggcatgaaa aagaacgcga agcggcagag
     3781 ttgtcggcaa aacaaagggt cggggaagag gaacgccgtc aaacagccca atcccagccg
     3841 caacgccgca aacgccgtgc cgcaccgcag gattatatgg cagcttccca agatcgtcct
     3901 aaacgccgcg gacaccgttc ggtacagcaa aacaatgtgg aaattgccca agcccaagca
     3961 gaacttgcgc gccgacagca ggaagaacgc aaagcggcag agttgttggc aaaacaaagg
     4021 gcagaagccg aacgtgaagc tcaagcattg gctgcgcgcc gaaaagcaga agccgaagag
     4081 gcaaaacgcc aagctgccga acttgcacat cgccaggaag cagaacgcaa agcggcagaa
     4141 ttgtcggcaa accaaaaagc cgcagctgaa gcccaagcat tggcggcacg ccaacaaaaa
     4201 gcccttgcgc gtcaacagga agaagcacga aaagctgcag agttagccgt caaacaaaaa
     4261 gcggaaaccg aacgcaaaac cgccgaactt gccaagcaaa gggcagcagc cgaagcagca
     4321 aaacgccagc aagaagctcg ccagaccgcc gaacttgcac gtcgtcaaga agcggaacgc
     4381 caagcagcag agttgtcggc aaaacaaaaa gccgaaacag atcgagaagc ggcagaatcg
     4441 gcgaaacgaa aagctgagga agaagagcac cgtcaagcag cccaatccca gccgcaacgc
     4501 cgaaaacgcc gtgccgcacc tcaggattat atggcagctt cccaaaatcg tccgaaacgt
     4561 cgcggacgca gatctactct gccggcaccg ccctcgccat catttgattc gtcagcttac
     4621 gcagcaccca gggccttgca taatccggac tggtatgaaa atgactatga agaaatcccc
     4681 ttggatgcgt tggaagatga aaatgtatct gaatcagttg acacatcaga caaacagcct
     4741 caagacaata cggaacttca tgaaaaatat gaaaatgatt atgaagaaat ccccttggat
     4801 gcgttggaag atgaagatgt atccgaatcg gttgacacat cagacaaaca gcctcaagac
     4861 aatacggaac ttcatgaaaa agttgagaca gtgagtttgc aaccaagagc cgcgcagccg
     4921 cgagcccaag ccgccactca gccgcaagcc caagccgccg cgcaagccga tgcagtcagc
     4981 accaatacca actcggcttt atctgacgca atggcaagca cgcaatctat cttgttggat
     5041 acaggtgctt cattaacacg gcacattgca caaaaatcac gcgctgatgc cgaaaaaaac
     5101 agtgtttgga tgtcgaacac cggttatggt cgtgattatg cttccgcaca atatcgccgg
     5161 tttagttcga aacgcacgca aacacaaatc ggcattgacc gcagcttgtc cgaaaatatg
     5221 cagataggcg gcgtattgac ttactctgac agtcagcata cttttgatca ggcgagcggc
     5281 aaaaatactt ttgtgcaagc caacctttat ggtaagtatt atttaaatga tgcttggtat
     5341 gtggccggcg atattggtgc aggcagcttg agaagccggt tacaaacgca gcaaaaagca
     5401 aactttaacc gagcaagcat ccaaaccggc cttactttgg gcaatacgct gaaaatcaat
     5461 caattcgaga ttgtccccag tgcggggatc cgttacagcc gcctgtcatc tgcagattac
     5521 aagttgggta acgacagtgt taaagtaagt tctatgtcag tgaaaacact aacggccgga
     5581 ctggattttg cttatcggtt taaagtcggc aaccttaccg taaaaccctt gttatctgca
     5641 gcttactttg ccaattatgg caaaggcggc gtgaatgtgg gcggtaattc cttcgtctat
     5701 aaagcagata atcaacagca atattcagca ggtgccgcgt tactgtaccg taatgttaca
     5761 ttaaacgtaa atggcagtat tacaaaagga aaacaattgg aaaaacaaaa atccggacaa
     5821 attaaaatac agattcgttt ctaaaatact aaattcatag caaaataaaa tgccgtctga
     5881 actcaggctt cagacggcat ttttatagtg gattaacaaa aatcaggaca aggcgacgaa
     5941 gccgcagaca gtacagatag tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa
     6001 tccactatat cagacggacg aggttacagt ttaatccctt tgagcttcgc gacggtattg
     6061 atgtctttgt cgccacgacc tgagaggttg accaaaatca cttggtcttt acccattttc
     6121 ggcgcatttt tcaccgccca agcaacggcg tggctggatt cgagcgcggg gatgatgccc
     6181 tcgaagcggc agagcaagtc aaaggcttcg agtgcttcgt cgtctttggc aacagtgtat
     6241 tcgacgcgct tgatgtcgtg cagatggctg tgttccgggc cgatgccggg gtaatccaag
     6301 cctgcggaaa cagagtgcgt acccaaaacc tgaccgtttt cgtcctgcat cagatagctg
     6361 cggaaaccgt gcaatacgcc aatcggtgcg cccgaagtaa tcggcgcggc gtgatcgggg
     6421 gtgttcacgc ccaaaccgcc agcctccacg ccgacgaggc gcacgttttc ttcgccgata
     6481 tacgggtgga acaaaccgat ggcgttcgat ccgccgccca cgcaggcaac ggcaacgtcg
     6541 ggctgtctgc cgatggcttc ctgcatctgc gctttagctt cgttgccaat cacgcattgg
     6601 aaatcgcgca ccatttcggg atacggcgcg gggccggcgg cggtaccgat gatgtagaac
     6661 gtgtcgtcca cgcgggcgac ccattcgcgc atggcttcgt tcatcgcgtc tttcagcgtg
     6721 cggctgccgc tttctacacc gaccacgttc gcacccaata atttcatacg gaacacgttg
     6781 ggcatttggc gttggatgtc gtccgcgccc atatacacgt cgcaagtcat accgaagcgt
     6841 gcggcaacgg tggcactcgc cacgccgtgc tgacccgcgc cggtttcggc gatgacgcgt
     6901 tttttaccca tgcgttttgc caacagtgcc tgaccgatgg tgttgttgac tttgtgcgcg
     6961 ccggtgtggt tcaagtcttc gcgcttcaac cagatttgcg cgccgcccag atgttcggac
     7021 aaccgcgcgg cgtggtaaac ggggctgggc ctgccgacat aatgtttcaa atcatggcgg
     7081 aacgcttccc aaaattcagg atcgtttttc gctgccttat aggcatccgc cagctcttgc
     7141 aaggcgggaa tcagggtttc ggagacataa agcccgccgt gttcgccgaa aaagcccttc
     7201 tcgtcgggcg cgtggtagtt cttcatcgga ttcttccttg tgcttcaaat tttcagacgg
     7261 catttccgtt tttgcaaaca tgccgtctga acgggaaatt gtcggcaatt ataactgttt
     7321 ttcacacaag cccgccattt caaaaaacgc ccgatacgcc gccgcttttt tcgccaaact
     7381 caaaggatag gcgcgcgaag tcgaaggcag gcgcgtcagc gtcaaatccc gtccggcaaa
     7441 cggaaacggc acggtttcgc ccgttttcgg cattttgata ccgccgccct gaatatcgag
     7501 caggatttcc gtcgccttgc cgcctgtcgt acaaatatgg cggcactcgg gtatttttgc
     7561 caacaccgcc gccaaatcga cggtttcaac tacctttaaa aacttgtcgg acgcattgcc
     7621 gtgctgacgt accgccttca aaacggtcgg acaggacgca atcccccgtt cgtgcaaaaa
     7681 cgccttgatt ttctcggcat caaacgcttt ttcagacaac ctttggaaat gcgccgcatc
     7741 attaaaaaac accagcccgt aaacgcgcca catatcgttt tggaaattcg gataatgaaa
     7801 ctgcatcgcg cgtttgtctt ccttgggcgg aaacgtcccc atcatcatga ccgcagcctt
     7861 cggcggcaac acggaatcga agggatgggt ttcgatttca agcagatttt cagacacgtt
     7921 caatcctcca acaggcaaaa cggcagacag ccggtttttg caccaaaccg ccaaacacgg
     7981 caagccttca aacagcatta tcacgccctg ccgttttcgg ttacaatgcc gacatcaaac
     8041 ggatactgta aacacattta tgtttcaaca cacaggacga cacataaagc accgccctat
     8101 gtgccgtcct gatttggaag gagttacgcc cctcccaaat agagtctgat tctaccgccc
     8161 taaagggcgg ggtttcaacc gaaaaggaaa cacgatgaaa gaacacaaag cccgcaagcg
     8221 tttcgggcag aattttttgc aggacacgcg gattatcagc gatattgtca acgccgtgcg
     8281 cccgcaggcg gatgatgtcg tgattgaaat cggtccgggt ttggcggcga ttaccgaacc
     8341 tttggcgaaa aagctgaacc gcctgcacgt tgtcgaaatc gaccgcaaca tcgtatgccg
     8401 tctgaaaacg ctgccgtttg cggataaact ggtgattcac gaaggcgatg tattgcagtt
     8461 tgatttcaac ggcatcgcag gcaaaaagaa aatcgtcggc aacctgccct acaacatttc
     8521 cacgccgctt ttgttcaagc tggcggaggt ggcggacgat gtcgtcgata tgcactttat
     8581 gctgcaaaaa gaagtggtcg agcgtatggt tgccgcgccg aaaagcaacg actacggccg
     8641 cttgggcgtg atgctgcaat attttttcga tatggaaatg ctgattgacg taccgcccga
     8701 atcgttcgac cctgcgccga aagtggattc cgccgtcgtc cgtatgattc cggtgaaaca
     8761 ccgcatcggc aaggcggacg atttcgagca tttcgccaaa ctggtgaaac tcgccttcca
     8821 ccaacgccgc aaaaccatac gcaacaacct gaaagagctt gcaggcgacg atgatttgca
     8881 ggctgtcggc atcaatccgc aagaccgtgc cgaacacatc gcgccggaaa aatacgtagc
     8941 gttgagcaat tatctggcgg gcaaggccgt ctgaaaaggg agtaagccga tgaagaaata
     9001 cctgctttta cccctgatac tgccgcttgc agcctgcctg accaatccga acgaccgttt
     9061 ttttgacggc agacgctaca cgcttcatca tatggagctc ccgtaacgac aacctgcaac
     9121 ggcaaatgcc gtctgaaaga aaagacatga tcaaattcaa aaacgtacac aaacatttca
     9181 aagacctgca cgtcatcaac ggcgtgaact tggaaatcaa aaaaggcgaa gtggtcgtcg
     9241 tctgcgggcc ttcgggcagc ggcaaatcca cactcatccg caccgtcaac cagcttgaaa
     9301 gcatcgaaag cggcgagatt tgggtggacg gtgtcaacgt tgccgaccca aaaaccgatt
     9361 tgaacaaaat ccgcgaagaa gtcggctttg tgtttcaaag tttcaacctc tacccgcacc
     9421 tgaccgtgtt ggaaaacatt accctcgcac cgatgaaggt taaggggcaa aatgcggaac
     9481 aggcggagaa aaaggcaatg gagcttttgg aacgcgtcgg actggcacac aaaaaagacg
     9541 ccttcccgtc ccaactttcc ggcggccagc agcaacgcgt ggcgattgcg cgcggtttgg
     9601 cgatggaacc gcgcgtaatg ttgtttgacg aaccgacctc tgcactcgac cccgaaatgg
     9661 tcggtgaagt gttgaaagtg atgaaagact tggcggaaag cggcatgacg atgatgtgcg
     9721 taacccacga aatgggattt gcccgcgaag ttgccgaccg ggtacttttt gtcgataaag
     9781 ggcaaatcct cgaagacgaa acgcccgaag cgttttttac caacccaaaa cacgaacgcg
     9841 ccaagcagtt cctgcagcag gtcatgaccc actgatgcta tgccgtctga aaaccgaacg
     9901 ttcagacggc atagcccgac accgcaagat gattgagaaa gaaaaccatg tattttgttg
     9961 accgcaccgc cgtcgtcctc aagccgaccg cgcgcttttt ggaatggctc aaaagcaccg
    10021 acgaaaatat gcccgacctg accatagaac agcttcgtgc caactgttcc gtcttcctcg
    10081 ttccgcagtt cgacgaaccg gaagccgtcg tttcctattt cgacgaacgc taccgccaga
    10141 ttttcgaggc ggagctggca ggttgggaca tcgataagga taaatggccg caagatatgg
    10201 ggctgaaggc gttttgggaa tttttcgaca tcgaaatcca cgatatggtg ttggacatgg
    10261 aagaagcgga attgaacatt acccccgtgt tcgacaacat gatgtaaggc tgtgcgcgcc
    10321 tttcagacgg cattgaggcc gtcccgcatc ctgaacatcc tgactgtatc gcttcacgcc
    10381 gcctcccttg ccgtctgcct gacttggttt tacggctgga tgatgtggtt cggtttggcg
    10441 gctttggtgg caagttacgc atactcgctc aggataacga atctgaaaca ccgccatgcc
    10501 ataaccgcca ttaccataga ccgcgacgga caggcggaaa tcgtatccgg caaagacaaa
    10561 acagcggcaa cacttgcggg cagcagtatg gttacgcctt atgccttatt cttacaatgg
    10621 gacacaggcg gcacaaccgt cagccaatgc atcatacccg acatgacgga caaaaaatcc
    10681 taccggcggc tgaaagtctg gatactttgg cggcagccga aaaaaaccgc cgaaaccgat
    10741 acaccaagct gatttcagac ggcatttgaa cacggaacac tcaatgaaaa cactgcaaga
    10801 ctggctctcg catttggaaa ccgcccacag cggcggcttg atcgatatgg gcttggaacg
    10861 cgtaggcgaa gtgaaaaaac ggatgaagct cacgccgcaa tgccccgtcg tcgttgtcgc
    10921 gggaacaaac ggcaaaggtt cggtctgcgc ctacctgaca cagatttaca aacaggcggg
    10981 ctacaaaacc ggcacactga ccagcccgca tttattgcgt tacaacgaac gcatcgccat
    11041 caacgccgaa cccgtttcgg acgatacgat tattgcctct ttcgagcgca tcgaagcggc
    11101 gcgcagcgaa atatcgctga cttattttga attcaatacg ttggcggccg tcgacatctt
    11161 catgcgcgaa caggttgacg tgatgatatt ggaagtcggc ttgggcggac gcttggacgc
    11221 ggtcaacgca tttgacggcg attgcgcggt ggttactagt gtagatttgg accatcaggc
    11281 gtttttgggc gatacggtcg agcaggtcgg ctttgaaaaa gcaggcgtgt tccgcagcgg
    11341 caaacccgca atctgcggtc aaaaccccgc gcccgaatcg ttgctttcac acgccgaagc
    11401 cataggcgcg aaactgctga tggtgcagcg cgatttcgag tttcacgcga tggaaaacat
    11461 ccaatggaac taccgcttcc ggccgcaaca ttcagacgac cccgcgcgca accgcaatgc
    11521 cctgcccttc cccgcattgc gcggcacata ccaactttcc aacgccgcct gcacgttgac
    11581 cgtattggaa tgcttgaacg ccaaattgcc ggtggacatc ggcgccatca aacgcggatt
    11641 gctgctggtt gaaaatcccg gacgcttcca agtcctgccc ggccgtccgc tgaccgtttt
    11701 ggatgtcgga cacaatcccc acgctgcccg cgccctgcgc cgcagcctga ttaatttggc
    11761 gtttgcgcaa aaacgcaccg ccgtattcag tatgctgtcc gacaaagaca tagacggcgt
    11821 gttggaaatc gttaaagacc agtttgacga gtggtatatc gcaccgttgg atgtaccgcg
    11881 cggcatgacg ctcgagagcc tgcaaaacaa actgcacgaa cacagcatcg aaaacataca
    11941 gacattcgcc gccgtccgcg aagcataccg ggcggcagcc tcaaaagcgg gcgagaacga
    12001 cagaatcgtc gttttcggct catttcatac cgttgccgat gtcatgtcgg tactttaaaa
    12061 ggaaacttct atgtccgaaa acaaacaaaa cgaagtcctg agcggttacg aacaactcaa
    12121 acggcgcaac cgccgccgcc tcgtaacggc aagttgcctg gttgccgcct cctgcatcct
    12181 gctggcagcc gccctcagtt ccggccctgc cgaacagact gccggcgaaa caagcggcgt
    12241 agaaaacaaa gcggcaggtg cggcacaaac ccctgccttg aaatccgccg ccgacaaacc
    12301 gcaggacttg gcaggcgaag acaagccttc tgccgccgac agcgaaatca gcgagcctga
    12361 aaacgtaggc gcgccgctgg tgctgattaa cgaccgcctc gaatacagca acatcaaagg
    12421 tttggaagca tccgagaaac tgcaacaggc agaaaccgcc aaaaccgcac cgaagcaggc
    12481 aaaacaacgc gctgccgaaa aagtgccggc aactgccgac agtacggata cggtagcggt
    12541 tgaaaaaccg aaacgcactg ccgaaacaaa accgcaaaaa gcggaacgca ctgccaaagc
    12601 caagcccaaa gccaaagaaa ccaaaaccgc cgaaaaagtt gccgacaaac cgaaaactgc
    12661 cgccgaaaaa accaaaccgg atacggcaaa atccgacagc gcggtaaaag aagcgaaaaa
    12721 agccgacaag gctgaaagca aaaaaacagc cgaaaaagac cgttcggacg gcaaaaaaca
    12781 cgaaacggca caaaaaaccg acaaagcgga caagaccaaa accgccgaga aggaaaaatc
    12841 cggtaaaaaa gccgccattc aggcaggtta tgccgaaaaa gaacgcgcct taagcctcca
    12901 gcgcaaaatg aaggcggtgg gtatcgattc gaccatcacc gaaattatga ccgacaacgg
    12961 caaagtttac cgcgtcaaat caagcaacta taaaaacgca agggatgccg aacgcgattt
    13021 gaacaaattg cgtgtacacg gtatcgccgg tcaggtaacg aatgaataac ctccccgttg
    13081 ccgacctcct cgtcttcgcc atcatcgccg cctgcatcgt gctatccgcg atgcgcggcg
    13141 tgattgcgga ggcaggttcg atggcggcat gggtggtttc cttctttttt gccaaactct
    13201 ttgccgcacc cttcgccgac ctcgcctttg cctcgttcca accccgcctg tttgcattgg
    13261 ctctgtcgtt catttccctg ttcgtcattg cctgtctgat ccagaaaatg ctccgttcgc
    13321 tgctgaccgg cgcagtttcg gcggtcggtt tgggctttgc caaccgcatt ttgggcggcg
    13381 tattcggtgc attgaaaggc gttttgattg ttaccctgct ggtcatgctt gcttcaaaaa
    13441 ccgacctgcc cgataccgaa gagtggcagc agtcctatac cataccgttt ttcgtatcgc
    13501 tttccgaagc cgtgttgaac catagcggcg gcacggcgga aactccggaa gacgattgat
    13561 tgccatacgg atatttcaga cggcattctc ttttatgccg tctgaaatgt tttttccttt
    13621 cctcagccat atcacagatt aaatcttatc ctccataaac ccacctatct gtccttcgct
    13681 caaatattgt caacaattcg ggcaaaaagc cgccgtttgc tttataattc cgttttaccc
    13741 ttaaacctcg aatcggagaa gacgatatgt gcggcgtatt aggtttggtc agtcatgaac
    13801 ccgtgaacca gcttctgtac gacggtttgc agatgttgca gcacaggggg caggatgcgg
    13861 cgggcattgc aacggcggaa ggcggcacct tccatatgca caaaggcaaa gggatggtgc
    13921 gcgaagtgtt ccgcacgcgc aatatgcgcg atttggtcgg caacgccggc atcgcccacg
    13981 tccgttatcc cacggcgggc aacgccggca gcagcgcgga ggcgcagcct ttctacgtca
    14041 gctcgccgtt cggcatcgtt ttggcgcaca acggcaacct caccaacact gccgaactgt
    14101 atgaaaacgt gtgcaacaaa cacctgcgcc acgtcaacac cagctccgat tccgaagtct
    14161 tgctcaacgt attcgcgcac gaattacgcc gcgaagtctc taaaaacgcc gacccgcacc
    14221 ggctcaatgc cgacaatatt ttcaacgccg ttgccgaagt ccaccgcctg gtgcgcggcg
    14281 catacggcgt ggttgccatg attgcgggct acggtatgct tgccttccgc gacccttacg
    14341 gcatccgccc gctggtattg ggttcgcaaa ccgacagcga aggcaggaaa tcctatgccg
    14401 tcgcctccga atccgtcgcc ttcaacgcgc ttgcctacga tttggaacgc gatatccggc
    14461 cgggcgaagc ggtatttgtc ggctttgacg gcacaatcat cgcccgacaa tgcagcgata
    14521 aggccaaact cagcccctgc ctttttgaat acgtctattt tgcccgtccc gactccgtga
    14581 ttgacggcgt gtccgtttac cagtcgcgct tggatatggg cgtgtccttg gcggaaaaaa
    14641 tcaaacgcga gctgcccgtg gacggcatcg atgtcgtgat gcccatcccc gacaccagcc
    14701 gccccagcgc gatggagctt gctgtccacc tcaaaaagcc ttaccgcgaa ggtttgatta
    14761 aaaaccgcta tatcggccgc acctttatta tgcccggcca ggcgacgcgc aaaaaatccg
    14821 tgcgccaaaa actcagcccg atggaaaccg aatttgcagg caaaagcgtg ttgctggtgg
    14881 acgactccat cgtgcgcggt acgaccagcc gtgaaatcgt cgaaatggta cgcgcggctg
    14941 gcgcgcgcaa agtctatatc gcctccgccg cgcccgaagt gcgctatccc aacgtatacg
    15001 gcatcgatat gcccacgcgc gaagagttga ttgccaatgg gcgcagcgcg gcggaaatcg
    15061 ccgccgaaat cggcgcggac ggcatcgtgt ttcaagactt gggcgatttg gaagccgtcg
    15121 tcaaagcact caacccgaaa atcgaatcct tcgattcgtc ctgtttcaac ggcatttatc
    15181 agaccggcga catcgatgat gcctacctcg accgcctgtc tgccgagaaa tccggctgcg
    15241 gcggtctgaa aatccacccg agcaggatgg aacacagcat cagcatcagc gatgcgggcg
    15301 acgaagaata aggcttccca aaccgcgatg ccgtctgaaa tccgaacgcc gggttttcag
    15361 acggcatttc ccatatcggc atcacatttc ccgtttcatc cgcacacaat atgagtaccg
    15421 aaaccaaaaa ctacattacg cccgccggat ggcaggcatt gaaagacgag ctttatcaac
    15481 tggtcaacaa agaacgcccc gaaatcgtcc aaatcgtcaa ctgggcggca ggcaacggcg
    15541 accgcagcga aaacggcgac tatctttacg gcaaacgccg tatgcgcgaa atcgaccgcc
    15601 gcatccgttt cctgaccaag cgtttggaag ccgccgtcgt cgttgatccc gaattgcgcg
    15661 aagcgaccga ccaagtgttt ttcggcgcga ccgtcggtct gttgcgtggc gacgggcgcg
    15721 agcaaaccgt caaaatcgtc ggcatcgatg aaatcgatac cgcgcaaaac aaaatttctt
    15781 ggatttcgcc gctggctcgt tgtctgatta aagcgcgcga aggcgacgaa gtggttttga
    15841 acacgccgga agggcgtgag gaaattgaaa tcctttctgt cgaatacatc aaaatcgact
    15901 gatcgggaat gccgtctgaa acggtgcaag cgcgccgttt cccaaataaa ggaataccga
    15961 atgaggaaaa tccgcaccaa aatctgcggc atcaccacac cggaagacgc atcggccgcc
    16021 gtagcggcag gtgcagatgc cgtcgggctg gtctttttcc aaggcagcag ccgggccgtc
    16081 gatattgccc gcgccaaaaa aatcaccgcc gcactgccgc cgtttgtcag cgttgtcgcc
    16141 cttttcgtca acgaaagcgc gcaaaacatc cgccgcatcc ttgccgaagt gccgatacac
    16201 atcatccaat tccacggcga cgaagacgac acattctgcc gtcagttcca ccgcccctat
    16261 atcaaggcca ttcgtgttca gacgacatca gacatccgaa acgccgccga ccgcttcccc
    16321 gacgctcagg cactgctttt cgatgcctac catccttcgg aatacggcgg caccggacac
    16381 cgcttcgact ggacgctgct ggcggaatat tcgggcaaac cgtgggtact tgccggcggg
    16441 ctgacccctg aaaacgtcgg cgaagccgtc cgcatcaccg gagcggaagc ggtcgatgta
    16501 tccggcggcg tggaagcatc gaaaggaaaa aaagatgccg ccaaagttgc cgcctttatc
    16561 gcaaccgcca accgcctatt ccgttaaagc aacaaaaatt gccgctggaa tga
//
LOCUS       DAWHRA010000033        16609 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWHRA010000033 DAWHRA010000000
VERSION     DAWHRA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.033"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            118..1293
                     /locus_tag="WIB30_000802"
     CDS             118..1293
                     /locus_tag="WIB30_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ3796897.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPTTKAVIVVHLAGMPAEMDGIMALAKEHNLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYNHEHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKRLPEWTARRRENAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTNDEIAFCKENIEAVLAEATR"
     gene            1341..3251
                     /gene="pglD"
                     /locus_tag="WIB30_000803"
     CDS             1341..3251
                     /gene="pglD"
                     /locus_tag="WIB30_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ3796898.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFTAFWFTQSLKADYSNEW
                     FDPANWQSFLLTALLTIGLFVRMGLYRAVTRFVSFHVLTTAFAGSLVSAVLFFLNTLI
                     FEEKLRLALPVVYFLLLLVSVTGSRMVIRGLLSDHQKKHMTPVIIYGAGRSGRQLLEA
                     VKQIREYSAVAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            3307..4599
                     /locus_tag="WIB30_000804"
     CDS             3307..4599
                     /locus_tag="WIB30_000804"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ3796899.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAEEHAVENIGNYSNPQGDAALIDALTTFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(4651..5487)
                     /locus_tag="WIB30_000805"
     CDS             complement(4651..5487)
                     /locus_tag="WIB30_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ3796900.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKKLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGLLASAGSDFHRLNDFSGGILGACPELPENCKPVWTHFS
                     RH"
     gene            complement(5883..6086)
                     /locus_tag="WIB30_000806"
     CDS             complement(5883..6086)
                     /locus_tag="WIB30_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796901.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(6064..6297)
                     /locus_tag="WIB30_000807"
     CDS             complement(6064..6297)
                     /locus_tag="WIB30_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ3796902.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            6837..10271
                     /gene="dnaE"
                     /locus_tag="WIB30_000808"
     CDS             6837..10271
                     /gene="dnaE"
                     /locus_tag="WIB30_000808"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ3796903.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLVAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSSDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVRDKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMEAEPQIQELIEVEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDLPRWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            10419..10568
                     /locus_tag="WIB30_000809"
     CDS             10419..10568
                     /locus_tag="WIB30_000809"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796904.1"
                     /translation="MHDTDSTKGKNGIFQSIGIKTALSLQKYQIDKPGKEIRPCRLKK
                     RPPVR"
     gene            complement(10705..11637)
                     /locus_tag="WIB30_000810"
     CDS             complement(10705..11637)
                     /locus_tag="WIB30_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ3796905.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEIPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMNNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFAAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLDGIDVCLNIGALERGSGCKRREERDMAGLSERMAAELDRHG
                     AAVFFREYPNADHGNVSFYSLTDCVEYLRKAWQR"
     gene            complement(11707..13821)
                     /locus_tag="WIB30_000811"
     CDS             complement(11707..13821)
                     /locus_tag="WIB30_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ3796906.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTEGLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVRKRPTKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRRETFYAAADWDINPDTVLGAGYLYQQRRLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMHSHDVFADLKHYFGNGGYGKVGMRYSDRKADSNYTFAGSKLNNTG
                     QADVAGLGTDIKQKAFAVDASYSRPFALGNTANEFVIGADYNRLRSTNEQGRSTLSKS
                     VALDGFRALPYNGILQNARAGNKGFNHSVTEENLDETGLYAKTVFRPLEGLSLIAGGR
                     VGHHKIESGDGKTLHKASKTKFTSYAGAVYDIDGSNSLYASASQLYTPQTSIGTDGKL
                     LKPREGNQFEIGYKGSYMDDRLNTRVSFYRMKDKNAAAPLDSNNKKTRYAALGKRVME
                     GVETEISGAMTPKWQIHAGYSYLHSQIKTASNSRDEGIFLLMPKHSANLWTTYQVTSG
                     LTIGGGVNAMSGITSSAGIHAGGYATFDAMAAYRFTPKLKLQINADNIFNRHYYARVG
                     SESTFNIPGSERSLTANLRYSF"
     gene            13981..14640
                     /locus_tag="WIB30_000812"
     CDS             13981..14640
                     /locus_tag="WIB30_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3796907.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRTLLEMPSETAVADITRTYSAHYLNPNNRNMSLFPDALPCLDKLKAQ
                     GYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLAQHY
                     A"
     gene            complement(14728..15696)
                     /gene="ispH"
                     /locus_tag="WIB30_000813"
                     /gene_synonym="lytB"
     CDS             complement(14728..15696)
                     /gene="ispH"
                     /locus_tag="WIB30_000813"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ3796908.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(15725..16222)
                     /locus_tag="WIB30_000814"
     CDS             complement(15725..16222)
                     /locus_tag="WIB30_000814"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ3796909.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
     gene            complement(16428..16532)
                     /locus_tag="WIB30_000815"
                     /pseudo
     CDS             complement(16428..16532)
                     /locus_tag="WIB30_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         3829 a         4870 c         4313 g         3597 t
ORIGIN      
        1 ccccgtacag accgattttg acaacacctg cggcgcgcgc ccgattcttc ggaacacgcc
       61 ccccttcaga cggcataggg tcggaaatgc cgtctgaaaa ccgacggaca aaccatcatg
      121 ctgaacactt tcctttcccc gtggccctgc ttcacccaag aagaagccga tgccgtttcc
      181 aaagtcctgc tgtccaacaa agtcaactac tggacgggca acgaatgccg cgaatttgaa
      241 aaagaatttg ccgcctttgc cggcacgcgg tacgccgtcg cccttgccaa cggcacgctg
      301 gcactcgatg tcgcgctcaa agcaatgggc ataggcgcgg gcgacgatgt gattgttacc
      361 tcgcgcacct tcctcgcttc cgcgtcctgc attgtgaacg cgggcgcaaa ccccgtgttt
      421 gccgatgtgg atttgaacag ccaaaacatc agcgcggaaa ccgtcaaagc cgcgctgaca
      481 ccgactacca aagccgtcat cgtcgtccac ctcgccggta tgcccgccga aatggacggc
      541 attatggctt tggcaaaaga acataatctt tgggtaatcg aagactgcgc ccaagcgcac
      601 ggcgcaaaat acaaaggcaa atccgtcggc tctatcggac acgtcggcgc gtggtcgttc
      661 tgccaagaca aaatcatgac caccggcggc gaaggcggta tggttacgac caacgacaaa
      721 accctgtggg aaaaaatgtg gtcgtacaaa gaccacggca aaagctacga tgccgtgtac
      781 aaccacgaac acgcgcccgg tttccgctgg ctgcacgaaa gtttcggcac aaactggcgt
      841 atgatggaaa tgcaggcggt catcggacgc atccagctca aacgcctgcc cgaatggacg
      901 gcgcgccgcc gagaaaacgc cgccaagctg gcggaaagcc taggcaaatt caaaagcatc
      961 cgcttggttg aagtcgccga ctacatcgga cacgcgcaat ataagttcta cgccttcgtc
     1021 aaacccgaac acctcaaaga cggctggacg cgcgaccgca tcgtgaacga actgaacgcg
     1081 cgcaaagtcc cctgctatca aggtagctgc tccgaagtgt atttagaaaa agccttcgac
     1141 aacacgccgt ggcgtccgaa agagcgtttg aaaaatgctg tcgagttggg cgacaccagc
     1201 ctgatgtttt tggtgcaccc gacgctgacc aacgacgaaa tcgcgttctg caaagaaaac
     1261 atcgaagccg tattggcaga agccacacga taacccttca gacggcatat gccgcctgaa
     1321 agcacatacc gccccacgat atgactctgg aaaccctgct tgccctaccg cgcaacatca
     1381 agaaaatctg tttcctcata cacgattttc tgatgatttt caccgccttc tggtttaccc
     1441 aaagcctgaa ggccgactac tcgaacgaat ggttcgaccc tgccaactgg cagtctttcc
     1501 tattgaccgc gctgttgacc atcggcctgt tcgtcagaat ggggctctac cgtgccgtta
     1561 cacgctttgt cagtttccac gtcctgacca ccgcctttgc cggcagcctc gtctccgccg
     1621 tgttgttttt cctcaatacg ctgatatttg aagaaaaatt gcgcctcgcc ctgcccgtag
     1681 tctactttct gctgctgctt gtctccgtaa ccggatcccg catggtcata cgcggccttt
     1741 tatccgacca ccaaaaaaaa catatgaccc ccgtcatcat ttacggtgcg ggacgatcgg
     1801 gcagacaact gctcgaggcc gtcaaacaga tacgcgaata ttccgcggtc gcctttgtgg
     1861 acgacaaccc caagctttgg cacaccgtca tctacgacct tgccgtttac cagcccgatg
     1921 ccatcgcctt cctcatcgaa cgttacggcg tggaaaaaat cctgctcgcc attcccagcg
     1981 cgactcagga acaacgccgc cgaatcatca gcaaactgga agcctatccg tgcgaagtgt
     2041 tgaccattcc cggaatgaaa gacctgatgg acgggaaaat cagcatcggc acgctcaaaa
     2101 aaatctccgt gtccgacctg ctcgggcgcg attccgtcgc acccgacgac cgtctgatga
     2161 gtgccgacat cgaaggcaaa accgtcatgg taaccggtgc gggcggctcc atcggttcgg
     2221 aactctgccg ccagattatc cgccgccgcc ccgaaaaact gctgctgttc gagttatccg
     2281 aattcgccct gtacgccatc gaaaaagaat tgcgcgaaac ctgcatccaa aaacgtctcg
     2341 ataccgaaat cctgcccttt ctcggctcgg tgcaaaaccg cacgctgctc gaacacgtca
     2401 tgaccgcctt ttccgttgcg accgtctatc acgccgctgc ctacaaacac gtccccatgg
     2461 tcgaatttaa caccgtcgaa ggcatacgca acaacatctt cggcacactc gagtgcgcgc
     2521 ttgccgccac ggcatcgggc gtaagaacct tcgtcctcat ctccaccgac aaagcagtcc
     2581 gccccaccaa caccatgggt gccagcaaac gcatggcgga actctgcctt caggcgctcg
     2641 ccgccgaacc cggacaaaaa acccgcttca gcatggtacg tttcggcaat gttttaggtt
     2701 cgtccggctc cgttgtcccg ctgtttgaaa aacagattgc cgaaggcggc ccgattaccc
     2761 tgacccaccc cgaaatcaca cgttatttca tgaccatacc cgaagccgcc caactcgtca
     2821 tacaggcagg cgcgatgggt acgggcggcg acgtattcgt cctcgacatg ggcgaatccg
     2881 tcaaaatcat cgaccttgcc cgccaaatga ttaccctaag cggcctcaaa cccaaaacac
     2941 ccgaacaacc cgacggcgac atcgaaatcc tcattaccgg actgcgtccc ggagaaaaac
     3001 tctacgaaga gctgctcatc ggcgacaacg tccgcaaaac cggccatccg cgcatcatga
     3061 ccgccaacga gaccatgctg ccgtggcacg agctctccgc cctactcgac cgcatccgtg
     3121 cggcctgcga ccgttacgac cagcaggcaa tccgcaccct gctcatcaac gccccgaccg
     3181 gctttgcccc gagcgacggt atctgcgacc tgctttgggt acgagaaaca cacagaaaaa
     3241 atgccgtctg aaccttcaga cggcataacg tacaaaccaa cctaccttac acacacggag
     3301 tttgacatgc agttttcagc attcggcgaa aaattcacgc aacacagcgg catcctccaa
     3361 ctgatggacg acctcggcga cgcgctcaaa agcgacaagc ccgtcaacat gctcggcggc
     3421 ggcaacccgg cgcgcattcc ggaaatcgat cgggcattcg ccgacatatt ctccaaactg
     3481 gcggaggaac acgccgtcga aaacatcggc aactactcca atccccaagg cgatgccgcg
     3541 ctgattgacg cgctgaccac cttcctcaac cgcgaatacg gctggaatct gaccgccgac
     3601 aatatcgcgc tgaccaacgg ttcgcaaaac gcgtttttct atttattcaa cctcttcggc
     3661 ggcaaattca acctttcaga cggcacatcc gcagaaaaag ccattttgtt gccgctcgcg
     3721 cccgaataca tcggctatgc cgacgtgcat gtcgaagggc agcacttcgt ttccgtcaag
     3781 cccaaaatcg aaaacgtcga acacgaaggc gaagcaggct tcttcaaata ccgcgtggac
     3841 tttgacgcac tggaaaacct acccgaactc aaagcgggca aaatcggcgc gatttgctgt
     3901 tcgcgcccga ccaacccgac cggcaatgtg ttgaccgacg gcgaaatggc gcgtttggac
     3961 gctttggcac gtgaacacgg gattccgctg attatcgaca acgcctacgg aatgccgttc
     4021 cccaacatca tttacagcga cgtaacgctg aattggcacg aaaacatcat cctctgcttc
     4081 agcctttcca aagtcggcct gccgggcgtt cgcaccggca tcatcgtcgc cgcgccggaa
     4141 gtggtcaaag ccgtcagcag cctgaacgcg attgtgaacc ttgcccccac gcgcttcggc
     4201 gcggccatcg ccgccccgct actggaaagc ggcgagatga aacggcttgc cgaccaagtc
     4261 atccggccgt tttaccgcaa tcaggcgcaa accgccgtct cgctgctcaa gcgcgagctg
     4321 ggcgcgtacc cgatgaaaat ccacaaaccc gaaggcgcga ttttcctgtg gctctggttt
     4381 gaaaacctgc ccgtttcttc gcaaaccctg tacgaaatgc tcaaagccga aggtacgctg
     4441 attattccgg gcgaacattt cttcgtcggc atcgacacgc aggattaccc gcacgcgggc
     4501 gagtgcatcc gcatgagcat cgcgcaggac gctcaaacgc tggaaaaagg cattgccgcc
     4561 atcggcaaaa ccgtccgaaa actgtacgac aacgtttaaa ccgcaaaaaa tgccgtctga
     4621 aaagttttca gacggcattt ttatccgcat tcaatgtcgg gaaaagtgcg tccacaccgg
     4681 tttgcagttt tccggcaatt cgggacacgc gccgagaatg ccgccgctga agtcgtttaa
     4741 acggtggaag tcgctgcccg cgcttgccaa caatccgaag cgttctgcca aaagcgcgta
     4801 gttgaggcgg tcgtttttgc aacagtttcc gctgtggact tcgatacccg caccgccgag
     4861 ttttttaaat tcttcaaaca gattgcgctt ggcggtggcg gacaaatcat aacgcatcgg
     4921 atgggcgatg accgccaacc cgcccgcgcc gttgacggcg gagacgcagt cttccagcgt
     4981 cgcccattcg tggcggacgg cgcaggattt gccgtcgccc aagtatttgg tgaacgcctg
     5041 ctgcttgttt ttgacgtgtc ccacccgaat gaggaactcg gcgacgtgtg tgcggctgac
     5101 catttctttg tttgccgcca gcgcgagcgc gccgtcgtat gcgcccgaaa tgccgcgttt
     5161 ttccagcttg gcggcgatgg cttcgaggcg tttcagacgg ccttgccgca cttgcgccaa
     5221 caggttttgc aggttttcgt cttgttcgtc aaagtctaag ccgacaacgt gtatggtacg
     5281 cccgcgccac gttacggaga tttccacgcc gttaatcagg cgcaaaccga gcctgtcggc
     5341 ttcggcacgc gcttcggcga tgccgccggt gtggtcgtgg tcggtcaacg ccagcagcgt
     5401 gcagccgttt tgatgcgcga ggcgcacaac ttcggcgggg gagagcatac cgtcggaaac
     5461 ggtggaatgg cagtgcaggt ctatcataag gcgttatgtg cgtggtaaat ggtgggcagg
     5521 gtgtttcaat attgtcgggc ggtgtggcgg cggtgatttc aaaaaacagg tgtatggcaa
     5581 atgcaaagga aaagccgcta tgcggtcatt cccgcgcagg cgggaatcca gactttgatt
     5641 tattggaaat gtttaaggtt aaccgctatt tcaaacttcc ggattcctgc tttcgaggga
     5701 atggcgatat gaccgttttc aattttaatc tactataaaa gactgtctga aaacgtggtt
     5761 ttatagtgaa ttaaatttaa accggtacag cgttggctcg ccttgccgta ctatttgtac
     5821 tgtctgcggc tcgccgcctt gtcctgattt ttgttaattc actatatcaa gccgaaccgt
     5881 tttcagacgg catcgtccga ccaacccgct tctttcaatt tccgccgttg cgcgtcgtag
     5941 cgggccttat ccgcccaata aatcgtctga atacacgcct cgccgcagtc gctgccgcag
     6001 cattcccacg actcgggtcg gacgggttcg tctaaaagcg gctcgcccaa aagggcttcg
     6061 gctttatgct tcagggtcat atccatcggc gatttccaag cgcgcaccgt caaactcgat
     6121 gacttcgccg ccgcgtattt tggcggtttt acgggtttcg gtttcgccgt tgcgcgacac
     6181 caacccttcg gcaatcaacg ccttcgcctg tccgccgctt tcggcaagtc cggccaattt
     6241 caagaggtcg cacagggcga tgtattcgtt gtcttcgaga tagacggtgg cttccataat
     6301 gttcccttgc agaaagaggc cgttattgta gcacctgccg ccgccgtacc caaaattacc
     6361 gcaaaacagg cagcacatcc cgccccgcct gttccgtaaa aatgccgtct gaaaccccat
     6421 atgccgtcat ccgatcaaag aaatcctgcc caacagcagg ctgcaaatcc tgttccccga
     6481 cgaatccgca ttgacgctga tgcacatcct caaacacgaa ctgcccgata cactggtcat
     6541 cggcatcagc catcaaaacg aaatcaaaaa cctgtttgaa cgtaaaatta atttaactgc
     6601 ttgccatccg tccgaataag gtatatagtt atttataact agtttgatag ttctttatat
     6661 ctatcaatac tccttgggaa gcctccacca cacggtagga ggcatttttt tgccgtcgta
     6721 aaagctcaaa aacatttgca ggtcatgccg tctgaatctg aaacggcatg acctacaccg
     6781 ccatctaaag acaaccctgc tacaatacgc cttttattgt tcacgccgat tttgccatga
     6841 ccgagccgac ctacatcccc ttgcgcctgc ataccgagtt ctcaattacc gacggtatgg
     6901 tgcggattaa aaaactggtt gccaaagcgc aggaatacgg tttgcctgct ttgggcatca
     6961 gcgatttgat gaacgaattc ggcttggtga aattctataa agcctgccgc ggcgcaggga
     7021 ttaaacccat cggcgcggcg gacgtgtgga taggcaatcc gaatgcgccc gacaagccgt
     7081 ttcgcgccat gctgattatc cgcaacgatg cgggctatct gcgcttgagc gagcttctga
     7141 cggcggctta tgtcggcaaa gaccgcaata tccatcatgc ggaactcaat cccgaatggc
     7201 tggaaaacgg cgacaacagc ggcttgattt gtttgagcgg cgcacattac ggcgaagtgg
     7261 gcgtgaatct gttgaacggc aatgaagacg cggcgcgtgc ggcggcgttg aaatatgcgg
     7321 catggtttcc cgatgcgttt tatttagagc tgcaacgcct gcccgaacgc cccgaatggg
     7381 aggcttgcgt ttccggcagc gtgaagctgg cggaggaact gggtttgccg gtggtggcga
     7441 cccacccgac gcagtttatg agcagcgatg atttcaacgc gcacgaggca cgggtgtgta
     7501 ttgcgggcgg ctgggtgctg acggacaaaa aacgtccgcg cgatttcaca ccgggccagt
     7561 ttttcattcc gccggaaacg atgctggagc gtttcgccga tttgcccgag gccttggaaa
     7621 acacggtaga aattgccaaa cgctgcaatc tgcacatcac attgggcaaa aacttcctgc
     7681 cccttttccc cacgcccgac ggtttatcgc gcgatgacta tctcatcaaa ctctcaaaca
     7741 agggcttgca ggaacgcatg gttcagcttt atcccgacga ggcggagcgt gcggcaaaaa
     7801 tgccggaata tcaggaacgt ttggattttg agctgaacat catcatccag atgaaattcc
     7861 ccggctattt ccttatcgta caagacttta tcaactgggc gaaaacgcac ggctgtccgg
     7921 tcgggccggg acgcggttcg ggcgcgggtt cgctggtggc gtattcgctg aaaattaccg
     7981 acctcgaccc gctgaaatac gcgctgctgt ttgaacgttt cttaaacccc gaacgcgtct
     8041 ccatgcccga cttcgacgtt gacttctgtc aggcaaaccg cggccgcgtg atcgaatatg
     8101 tgcgcgataa atacggcgct caggcggtca gccagattgt taccttcggc acgatgtcgt
     8161 ccaaagcggt catccgcgac gtggggcgcg tgttggagct gccgtttatg ctgtgcgaca
     8221 aactgtccaa gctgattccg ttggaagcca acaaacctct gagtttggaa aaagccatgg
     8281 aggccgagcc gcagattcag gaattgatcg aggtggaaga agcggacgaa ctgattacac
     8341 tggcgaaaaa gctggaagat ttgacgcgcg gtctgggtat gcacgcgggc ggcgtgttga
     8401 ttgcgccggg caagatttcc gattacagcc ccgtgtatca ggcggatgaa tccgcctcgc
     8461 ccgtatccat gtacgacaag ggtgacgtgg aagacgtggg cttggtgaaa ttcgactttt
     8521 tgggtctgcg caacctgacc attatcgaaa tggcgcagaa caacatcaaa aacaccaccg
     8581 gcgacatcat cgatgtcggc aaaattcctc tcgacgacca agccgcctac caaatcttcc
     8641 gagatgcgaa caccaccgcc gtgttccagt tcgagtcgac cggcatgaaa aaaatgctga
     8701 aaacggcgca cacgaccaag tttgaagaac tgattgcctt cgtatcgctc taccgccccg
     8761 gcccgatgga caacatcccc gacttcgtcg cgcgtatgaa ggggcaagaa ttccaatata
     8821 tccatccgct gctcgagggc atcctcgcgc cgacctacgg cattatggtg tatcaggaac
     8881 aagtgatgca ggcggcacag attatcggcg gctactcgct cggcggcgcg gacttgctgc
     8941 gccgcgcgat gggtaagaaa aagcccgaag aaatggtgaa acaccgcgaa atcttcgccg
     9001 aaggcgcggc aaaacaaggc atttcgcgcg aaaaatccga cgaaatcttc aactatatgg
     9061 aaaaattcgc cggctacggt ttcaacaaat cccacgccgc cgcctacgcc ctgatttcct
     9121 accagaccgc atggctcaag gcgcactacc ccgccgaatt tatggcggcg accatgtcgt
     9181 ccgaattgga caacaccgac cagctcaagc atttctacga cgactgccgc gccaacggca
     9241 tcgagttcct gccgcccgac atcaacgaat ccgactaccg ctttacgccg tatccggaca
     9301 tgaaaatccg ctacgcgctc ggcgcgatta aaggcacggg cgaagccgcc gtcgaatcca
     9361 tcaccgccgc gcggcaaagc ggcggcaagt ttaccggcct gttggacttc tgcgagcgcg
     9421 tcggcaaaga acacatgaac cgccgcaccc tcgaagccct gatacgcggc ggcgcgttcg
     9481 acagtatcga acccaaccgc gccatgctct tggcgaacat cgacctcgct atggacaacg
     9541 ccgaccaaaa agccgccaac gccaatcagg gcgggctgtt cgacatgatg gaagacgcca
     9601 tcgaaccggt gcggctcatc gacctgccga ggtggagcga atcggaaaaa ctcgccgaag
     9661 aaaaaaccgt catcggcttt tacctgtccg gccacccgtt cggcccgtat gcccaagaag
     9721 tccgccaaat cgcaccgacc aaattagacc gtctgaagcc gcaagacagc gtgcgcctcg
     9781 ccggattcgt taccgccgta cgcaccatga tgggcaagcg cggcaaaatc gccttcgtca
     9841 gccttgaaga tttgagcgga caggttgaaa tcatggtcgg cggtcagacg ttggaaaact
     9901 gcgccgactg cctcaaagcc gaccaagtgc tgattatcga atccaaagtc agccgcgacg
     9961 actacggcgg cggcgacggg ctgcgtattc tggcaaacca agtcatgacc ctgcaaacgg
    10021 cacgcgaacg ctacgcccgc agcctcagcc tcgccctcgc cccgcatcac gacatcggcg
    10081 gactggtacg gctgctcgcc gcccaccaac tgcccgacac gccgcgcatc ccgctgcaac
    10141 tgtcgtatgc caacgaaaaa gcgtcgggca ggcttcaagt gccgccgaaa tggacggtta
    10201 caccgagctc cgcattgttc ggcgaactgg aaacattgct cggcagccgg tcggtgcgcg
    10261 tcaactggta acccaaaata taaatgccgt ctgaagccca aaaaccggtt tcattcgtac
    10321 tttattcgaa tgattgaata aaagtaactg ccaagaaaaa cgtatttttt ggttatttcg
    10381 ccagtctaaa tagagcaacc gggacgattg atatccgtgt gcatgacaca gacagcacca
    10441 aagggaaaaa cggcattttc caaagtatcg gtatcaaaac cgccctttca ctccaaaaat
    10501 accaaatcga caaaccgggc aaagaaatca gaccgtgccg cctgaaaaaa cgcccacccg
    10561 tccgttaaat taacctatcc ctgtttcaga cggcctgaag cagggatttt tatatcaaaa
    10621 taaaatgaga aagggagcaa taacccttag gtagctcttg ttattttccg atgcaaaaca
    10681 aagcagtcat atatttaatt ccccctacct ctgccaagcc ttcctcaaat attcgacgca
    10741 atcggtcagc gagtagaacg agacattgcc gtggtcggca ttcggatatt cccggaaaaa
    10801 gacggcggcc ccgtgcctgt ccaactctgc cgccatgcgt tcggacagtc ctgccatatc
    10861 gcgttcttcc ctgcgtttgc aaccgctgcc ccgttctagc gcgccgatgt tgaggcagac
    10921 atcgatgccg tccagccggt tttcagacgg cataaaatcg agtatccgcc tgttgtgcca
    10981 ccaaatcgag ggggatacga gccaatgccg tctgaaacgg cggtgggaaa gcagggaata
    11041 cagtccgaac agtgcgccga acgagtgtcc gaatacggcg gtttcattgc ggttgagggt
    11101 gtagcggctt tctaaaaagg cggtcagctc gctgtcgata aaggcggcga agcggtctgc
    11161 ctgtccgaac tgctgccgtt cgtctgctgt ggcgttgtct ccaagcggcg gcgtgtagtc
    11221 ggcggcacgt tgtgccaaat cgcgcacact gcctgtcgtg tagccgatac cgacaatcag
    11281 gcagggggcg ttgcttcggg taacggggtt gttcatcagc gactgcatga tgttgaaaag
    11341 tgcggggaaa aaggcttcgc cgtcgaggac aaagaggacg ggatagcctt cagacggtat
    11401 ttcgccgagt gttgccgtct gaatccgata gattcgcccc gtgcaggtgg atttgatttc
    11461 ggtttcaaag gcttggggca gtatggcagg ttggaatgtg tcggtcggta tgggtttcat
    11521 gatgttcggc ttgtgggtca gactggcggc aagcggtaaa accgaaccgg caaagcggtc
    11581 tgcctgccgg ttcggtctat tttccttcgc aatgccatac tccagttgtg agagcatagg
    11641 gtatgccgcg cagcttgttg tagtttgatg atgctgcggc tgccgtttca gacggcatat
    11701 tggtctttaa aaactgtaac gcaggtttgc cgtcaggctg cgctccgaac cgggaatgtt
    11761 aaaggtgctc tcgctgccga cgcgggcgta gtaatggcgg ttgaagatgt tgtcggcgtt
    11821 gatttgcagc ttcagtttgg gcgtgaagcg gtatgccgcc atcgcatcga acgtggcata
    11881 accgcctgca tgtatccctg cagatgaagt aatgccgctc atcgcgttca cgccgccgcc
    11941 gatggtcagc ccggacgtaa cttggtaagt cgtccacagg tttgcgctgt gtttgggcat
    12001 cagcaggaag atgccttcgt cgcgcgaatt ggaggcggtt ttgatttggc tgtgcaggta
    12061 gctgtaacct gcatggattt gccatttcgg tgtcatcgcg ccgctgattt cggtctcaac
    12121 accttccatc acgcgtttgc ccaatgcggc gtaacgggtt tttttgttgt ttgagtccag
    12181 cggtgcggcg gcgtttttat ccttcatgcg gtagaacgaa acccgggtat tgaggcggtc
    12241 gtccatgtag ctgcctttgt agccgatttc aaactggttg ccttcgcgcg gtttgagcag
    12301 cttgccgtcg gtgccgatgc tggtttgcgg tgtgtagagt tgggaggcgg aagcgtacag
    12361 gctgttgctg ccgtctatat cgtaaaccgc gccggcgtag cttgtaaatt tggttttcga
    12421 agctttatgc agggttttgc cgtcgcccga ctcgattttg tgatgtccta cacgtccgcc
    12481 tgcaatcaac gacaaacctt ccagaggacg gaacaccgtc ttggcataca aaccggtttc
    12541 gtcgaggttt tcttcggtaa cggagtgatt gaaacctttg tttccggcgc gggcgttctg
    12601 aagtatgccg ttataaggca aagcgcggaa accatctaaa gcgacgcttt ttgacaaagt
    12661 cgaacgccct tgttcattag tactgcgcaa gcggttgtag tctgcaccaa tcacaaattc
    12721 gttggcggtg ttgcccaagg caaacggacg gctgtaactt gcgtcaaccg caaaggcttt
    12781 ttgtttaatg tccgtaccca aacccgctac gtcggcttgt ccggtattgt tgagtttgct
    12841 gcccgcaaac gtataattgg aatcggcttt ccgatcggaa tagcgcatac cgactttgcc
    12901 gtagccgccg ttgccgaagt aatgtttcaa atcggcgaac acgtcgtggc tgtgcatttt
    12961 aaatttgttc caatccgcgc cgacaaatac gtgttgcggc agggacggta atttgttatt
    13021 ggcatcggca ggcaggccgt tgtacggcgc gaggcggcgt tgctggtaaa gatagcccgc
    13081 gcccaaaacc gtatcggggt tgatgtccca atccgccgcc gcgtagaagg tttcgcgccg
    13141 gttgtttttc tcggcgggac gcggagacgc gccgacggtc tgcgccatca cgcggccgcg
    13201 cacgctgccg tctgaattga ggctgcccga tacgtccgcc tcggctttat attgtttgtg
    13261 cgtaccgaac cctgccgccg catgaccttg gaacgctttg gtcgggcgtt tgcgcaccag
    13321 attcacgatg ccgcccatct cgccgctgct gtcgaacagt ccgctcggcc cgcgcatcac
    13381 ttccacgcgg tcgaaggcga acaggttggg cagcgtgccg ttgatactct gcatctgcgc
    13441 gggcaggccg tcgatgttgt attcgctgta ttcgtaaccg cgcgcgtaaa ccgaagagcg
    13501 tccgtcgtcg ttgctcaaca cgcgcaggcc gggcgttttg cgtgccaact ggtcaaacgt
    13561 atcaacattg cggtctttga cctgctggtt ggtaatgatg ctgacggatt gcggaatttc
    13621 gcgcaaagaa gcggggattt ttgtaccgac ggtggcggca aacgagctgt aatcgccgtt
    13681 tttctcggtg gcaatcgcgt tgtaagaacg ctgaccctta atatggacgg tttccaaacc
    13741 ttccgtttgt gcggcaaaaa ccgaagacga gagtgctgcc aaaaccgtgg cggcggtcat
    13801 attgatgcgg aaaactgaca taaactgtcc cattcacata aatgataatg gttctatttt
    13861 aataaagcgc aacgcggctt gttcggaaaa acatatcgcg cagccgacaa attttgtcga
    13921 aaatgcgaca cgtctgcgtt ttccgcataa aatttgcttt tttactgcaa ccaacctgct
    13981 atgaccacgc ccaaactcat catcttcgac tgggacggca cgcttgccga tacgacccaa
    14041 cccatcatcg acaccatgcg ccgcagcttc gccgaatgcg gttttccgcc gcccgaagcg
    14101 gaacgcgtcc gcagcctgat tggctacagc ctgcccgaaa tcatccgcac cctgctcgaa
    14161 atgccgtctg aaaccgccgt tgccgacatc acacgcactt attccgcaca ttacctcaat
    14221 cccaacaacc gcaatatgtc cttatttccc gatgccctgc cctgtctgga caagctcaaa
    14281 gcacaaggat actggcttgc cgtcgccacg ggcaaagggc gggcgggttt ggacaacgcc
    14341 atcagtcaaa ccgccaccgg cggctattgg ctcgccaccg cctgcgcggg ggaatatccc
    14401 tccaaaccct cgcccgaaat ggtattcgga atctgcggcg aactgggact cgacccgaaa
    14461 gaggcattgg tcgtcggcga tacggcgcac gacctgcata tggcggcaaa cgcaggcgcg
    14521 gcggcagtcg gcgtggccac cggcgcacat tcgcgcgaac agctccttag cgcaccgcat
    14581 ctcgccgtat tggacggttt gtccgaactg cccggttttc ttgcacaaca ttacgcctga
    14641 ttggtttccg catccggcac acggcaaaaa tgccgtctga agcctgttca gacggcattt
    14701 gtgttgccca aacattcaac gcctgcgtca acgtttgcac aaatcgggtt tggtttcgcc
    14761 ctcgcggcgc aactctttgg gcaggacgaa cacaatgctt tcttccgcac cctcgccttc
    14821 gcgtacggtt tcgtgccccc atccgcgtat ggttgccagt acttcccgca ccaacacttc
    14881 gggcgcggac gcgcctgccg ttacgccgac tttgtttttg ccctcaaacc atgcgcgttg
    14941 caggtagcct gcattatcca ccatatacgc atcgattccg cgcgatgccg ccacttcgcg
    15001 caagcggttg ctgttggacg aattgggcga accgaccaca atcacgatgt cgcactgttc
    15061 tgccaactct ttgacggcgg tttgccggtt ggtcgtcgca tagcagatat cttccttgtg
    15121 cggattgcgg atattgggga aacgcgcgtt cagcgcggcg atgatgtctt tggtttcatc
    15181 gaccgagagc gtggtttggc tgacataggc gagtttgtcg gggtttctga cttcgagttt
    15241 tgccacatct ccgaccgttt cgaccaaaag cattttgccc ggcgcaagct gccccatcgt
    15301 tccttcgacc tcgacgtgcc ccttatgccc gatcatgatg atttcacagt cttgggcatc
    15361 cagtcgggcg acttccttat gcactttcgt caccagcggg caagtcgcat caaacacgcg
    15421 gaaaccgcgc tccgccgctt cttgccgcac cgccttcgat acgccgtgtg ccgaataaac
    15481 cagtgtcgcg cccggcggca cttccgccaa gtcttcaata aacaccgcac ctttttcacg
    15541 caggttgtcc acgacgaatt tgttgtgaac gacttcgtgg cgcacataaa tcggcgcgcc
    15601 gaactcttcc aaagcacgtt cgacaatact gattgcccga tccacaccag cgcagaagcc
    15661 gcgcggattg gcaaggatga tggttttctc gttcataagc ccggtatttc gttttcagac
    15721 ggcatcaata tttttcttct tgggttttac ggtggacgat gttgtccaac actgccaaca
    15781 ccgcgccgac gcagataaag ctgtcggcaa tattaaaggc gggataaaac caattttgcc
    15841 aataaaacaa taagaaatcg acgacatgac cgtgtatcag gcggtcgatg acattgccta
    15901 acgcaccgcc gataatcatc gccgcacccg ttttgccgag ggttgcaaac tcatcgcgca
    15961 agatggcgcg taccaaatac gcgctcaccg ccaccgccag caccaaaaaa aagtattttt
    16021 gccagccgcc ctgatcggca aggaagctga acgccgcacc cgggttgtac accaaggtca
    16081 aatcgaaaaa ggaaggaatg acattgacgc gttcccgata ctgaaacgac gacagcaccg
    16141 cccacttcga ccactggtcc agcacgatgg cggcaagtgc caatacccaa tagcgcgttt
    16201 tacttgaaac agatgaagac atatttttca acagccggta aaagagtacc attttacccg
    16261 aaaaccccct ttcctgtacc cgaaacggca aatgccgtaa tcttaaaacc cgtcattccc
    16321 gacaacaccg taatctcgaa acccgtcatt cccgcgtagg cgggaatcca gacctgtccg
    16381 cacagaaact tatcggataa aaacagttgc ccaaaccccg cgttctatag tggattaaat
    16441 tcaaaccagt acggcattgc ctcgccttgc cgtactattt gtactgtctg cggcttcgtt
    16501 gccttgtcct gatttaaatt taatccacta tagattccca cttccgtggg aatgacggtt
    16561 cagttgcatt ccgacaacac cgtaatcttg aaatccgtca ttcccgcgc
//
LOCUS       DAWHRA010000034        15780 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWHRA010000034 DAWHRA010000000
VERSION     DAWHRA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15780
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.034"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(130..1173)
                     /gene="galE"
                     /locus_tag="WIB30_000816"
     CDS             complement(130..1173)
                     /gene="galE"
                     /locus_tag="WIB30_000816"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ3796910.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQPPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDECQQLDLYRKNIGKLDNQYKRALAFTLMRRAMIRKMPYTEDMRPGDTANPYGA
                     SKAMVERMLTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAA
                     GKLPQLAVFGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRAS
                     SVLEIIRAFEAASGLTIPYEVKPRRAGDLACFYADPSYAKAQIGWQTQRDLTQMMEDS
                     WRWVSNNPNGYDD"
     gene            complement(1186..2166)
                     /gene="dcm"
                     /locus_tag="WIB30_000817"
     CDS             complement(1186..2166)
                     /gene="dcm"
                     /locus_tag="WIB30_000817"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ3796911.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSVPDNFIFVAAKLPSSSRLVTPYRLYWQKLLLKVF"
     gene            complement(2197..4470)
                     /locus_tag="WIB30_000818"
     CDS             complement(2197..4470)
                     /locus_tag="WIB30_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ3796912.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDGLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPEMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRNENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGTEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEIGDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4534..5184)
                     /locus_tag="WIB30_000819"
     CDS             complement(4534..5184)
                     /locus_tag="WIB30_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3796913.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5181..5978)
                     /locus_tag="WIB30_000820"
     CDS             complement(5181..5978)
                     /locus_tag="WIB30_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ3796914.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(5978..7141)
                     /gene="ctrB"
                     /locus_tag="WIB30_000821"
     CDS             complement(5978..7141)
                     /gene="ctrB"
                     /locus_tag="WIB30_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ3796915.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7156..8331)
                     /gene="ctrA"
                     /locus_tag="WIB30_000822"
     CDS             complement(7156..8331)
                     /gene="ctrA"
                     /locus_tag="WIB30_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ3796916.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8458..9591
                     /gene="neuC"
                     /locus_tag="WIB30_000823"
     CDS             8458..9591
                     /gene="neuC"
                     /locus_tag="WIB30_000823"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ3796917.1"
                     /translation="MKRILCITGTRADFSKLKPLLAYIENHLDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            9595..10281
                     /gene="neuA"
                     /locus_tag="WIB30_000824"
     CDS             9595..10281
                     /gene="neuA"
                     /locus_tag="WIB30_000824"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ3796918.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10282..11331
                     /gene="siaC"
                     /locus_tag="WIB30_000825"
     CDS             10282..11331
                     /gene="siaC"
                     /locus_tag="WIB30_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ3796919.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            11415..12902
                     /locus_tag="WIB30_000826"
     CDS             11415..12902
                     /locus_tag="WIB30_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HEZ3796920.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
     gene            complement(13398..14351)
                     /locus_tag="WIB30_000827"
     CDS             complement(13398..14351)
                     /locus_tag="WIB30_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796921.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGIVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGRHGEK
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            14732..15751
                     /gene="galE"
                     /locus_tag="WIB30_000828"
     CDS             14732..15751
                     /gene="galE"
                     /locus_tag="WIB30_000828"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ3796922.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGASKSMVERI
                     LTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKGGVAGVHLFNLGSGRAYSVLEIIRA
                     FEAASGLNIPYQIQPRRAGDLACSFADPSHTKQETGWETKRSLAQMMEDSWRWVSRNP
                     DGYGD"
BASE COUNT         4606 a         3634 c         3155 g         4385 t
ORIGIN      
        1 aggatttttc tcataaattt cagaggatag ccaaaaaata taaacagatt atagcagaca
       61 gaatgtgtgt ttttcagata aagaggccgt ctgaaaacat ctctttcaga cggcctgtat
      121 caggtcaact taatcgtcgt agccattcgg attattactc acccagcgcc atgagtcttc
      181 catcatttgg gttaaatcac gctgggtttg ccagccgatt tgcgcctttg cataggaagg
      241 gtcggcatag aagcacgcca aatcaccggc acggcgcggt ttgacttcat acggaatcgt
      301 caaacccgaa gctgcttcaa atgcgcggat gatttccaat accgaagaag cgcggccgga
      361 gcctaagttc aacaaatgcg tgcctgctac attacttttt gcctgcatag ccgcgacatg
      421 gccttctgcc aaatccatca catgaatata gtcgcgcatc cccgtgccgt cgggggtagg
      481 gtagtcatcg ccaaataccg ccaattgcgg cagtttgcct gccgccactt ggcagatata
      541 aggcaacaaa ttattcggga tgccgtttgg ctgctcgcca atcaagccgc tttcatgcgc
      601 gccgatcggg ttgaaataac gcaacaaaat cacgctccaa cgcggatcgg ctttttggat
      661 gtcggttaac atccgttcca ccatcgcttt ggacgcgcca taaggattag cggtatcgcc
      721 cgggcgcata tcttccgtat agggcatttt acgtatcatg gcgcgacgca ttaaagtaaa
      781 cgccaaagcg cgtttatact ggttatccag tttccctata tttttacggt acaaatcaag
      841 ttgttggcat tcatcgtgaa aatagaattt ttcggcataa cgctgactca taaaaccggc
      901 atgcgggctg cctgaaaaaa tcattgcgac atcatcgtca tttaatgttt catggttgtt
      961 ttctattaat gctaaagcaa tttggtaatt aattgccaaa atatcgttag taatcacacg
     1021 atagccgcgt tttttggctt cataggcaaa ggaacagcct ccactaaata catctgcaac
     1081 tgtatctaca tcagacggag gctggtcaca aatccaggaa gctattttct ctttattacc
     1141 gatatagtta attttcggat atttcttatt cattttcact catttttaaa atactttcag
     1201 caatagcttt tgccaataaa ggcggtacgg cgttaccaac ctgctgctgc tgggcaattt
     1261 tgctgccaca aaaataaaat tatcagggac agattgtaag gcagctaatt cacgaacggt
     1321 taaagcccga ttctgttcat agtgaaaaac tttgcgcata tctcctgtaa tacaaacggc
     1381 tggtttgttg ctgttgtaac ggatgtattt acggatatca cctgttttcg gacgtaatgg
     1441 ttcaggaata tcgttacggt tacctccatt tttaacaaat gccatttttt ctaacatttg
     1501 tgccgaatga ttcatagctt catgatttgc aacgtgtgga ttgctttcgc cagcagccag
     1561 ttttggaaaa tgtcctattg ctgatccaac agtctgatgg gaaatctgca aaggttcggg
     1621 aaaggaaatt ttgcctttat ccctcctccc gataaatatc actcggctac gtatctgagg
     1681 aacaccgaaa tcggctgcac tcagtatctt acattccacc gaataaccga tattctgaaa
     1741 tgcttgaata atctcaatac gtgttttacc tgaattgtgt gtatagagtc gcgctacatt
     1801 ttccataaca aaaaaatatg gttggacaat tttaactatt cggacaaact ctttaaataa
     1861 atggttgcgt gggtcatctg taaatgtccg tccaatcttt cctgccatac taaaaccttg
     1921 acaaggtggt cctccaataa tcaaatcaac tgcttgtccg ttaagacaat tgattaaatc
     1981 ttgttcggtt agtgtggtta aatctttttg cagtaattga tgatggggga agttggtacg
     2041 gtaagtctga caataatcag actccatttc aacagaaagc aattgttgga atccggcttg
     2101 ttcaaaaccc aaggataggc ctcctgctcc tgagaaaagg tcaatatagg taagtggtgt
     2161 gtgcataagt caaaatccat aaactcttcg attattttac cgcttcagct tcgcaaacgc
     2221 atccgccatc gccgaattgg ctggggcgcg gtcgttgcgt tggggtttgc ggccggctgt
     2281 ccgttcgcgg ctgcggtttt cagacggcat tttatgtttt gcgccgcccg gttcgtcatc
     2341 caagcgcatg gtcagcgcga tgcgttttct ggcggcatcg acttccagca ctttcacttt
     2401 cactacatcg ccggctttca ccacttcgcg cgggtcttgg acgaatttat tggatagggc
     2461 ggagatgtgc accaagccgt cctgatgcac gccgatgtcc acgaacgcgc cgaagttggc
     2521 aacgttggaa accacgccct ctaaaatcat accgacttgc aagtcgccga tttcgtggat
     2581 gccttcggca aacgatgccg tctgaaacgc gccgcgcgga tcacggccgg gtttttccag
     2641 ttcggacagg atgtccaaaa tggtcggcag gccgaagcgt tcgtcggtga agtcggacgc
     2701 tttgatttgc ttcacgcgct cgcggttgcc gatgagttcg gcggcgctaa tgccttgttg
     2761 cgccagcatt ttggcgacga cgggataggc ttcggggtgg acggcgctcg cgtccaacgg
     2821 ctctgtgccg ccgttaatcc gcaaaaagcc tgccgcctgc tcgaaggttt tttcgcccaa
     2881 acgcggtact ttcagcaatt ttttgcggct gtcgaacgcg ccgttttcat tgcggtaggc
     2941 gacgatgttt tgggcaaggg tctgattcaa accggaaatc cgcgccaaga gcggggcgga
     3001 ggcagtattc acgtccacgc cgacggcgtt cacgcagtct tcgaccaccg cgtccagcga
     3061 tttggcgagc tggctttggt tcacgtcgtg ctgatactgg cccacgccga tggatttggg
     3121 gtcgattttg accagctcgg caagcgggtc ttgcagcctg cgggcgatgg acaccgcgcc
     3181 gcgcagggaa acgtccaagt cggggaactc gcgcgctgcc agttcggacg cggaataaat
     3241 cgacgcgccg gcttcggaaa cgacgatttt gtgcagtccc atttccggca ttccacgcac
     3301 cagttcgccc gcgattttgt cagtttcgcg gctggcggtg ccgttaccga tggcgatgag
     3361 cttcacgccg tgctgtttaa tcaggcgcga cagcgttgcc aacatattgt tttcttgatg
     3421 caaatagaca atgacggtat ccagcagctt gccggtgtcg tccaccacgg cgcatttcac
     3481 gccgttgcgg tagccggggt cgagacccag cgtggtcagc cgtccggcgg gcgcagcaag
     3541 cagcaagtct ttgagattgc gggcgaacac ggtaatcgcg tcggtgtcgg cggcttcttt
     3601 caaacgattg agagcttcaa gttccaacga caaaaagatt ttcgcgcgcc aagtcaggcg
     3661 cacggtgtcg cgcagccatt tgtggccgtc tgaaactttg aagcggcggg cgatgatttg
     3721 ctcgtattcg ctttgctgcg taatcggcgt gtcgtcgggc tgatatttga gcgcgatgtt
     3781 caacacgcct tcgttgcgtc cgcgcaaaac cgctagcgca cgatggctgg gcatcgcacg
     3841 aataggttcg cggtggtcaa aataatcgct gaatttttcg ccttcggttt ctttgcctcc
     3901 aacaacttgc gcgtggattt cggcttcgtt ccacagcttg tcgcgcagcg tgccgatgag
     3961 ttccgcgtct tcggcaaact gctccatcag aatcgcacgc gcgccgtcca acgcggcttt
     4021 ggcatcgggg acgttttcgt tcaggtagcc ttgtgcggcg gcttccacgt cctgcgactg
     4081 ctcggcaagc aacacgtccg ccagcggctg caaaccgtgt tcgcgcgcga tttgcgcttt
     4141 ggtgcggcgt ttgggtttgt agggcagata caggtcttcc agcgcggttt tgttatcggc
     4201 ggcttcgatt tgcgcgcgga ggccgtctga aagcttgcct tgctcttcaa tgctttttaa
     4261 aacaacggct ttgcgctctt ccaactcgcg cagatattgc agccgctcgg caagccggcg
     4321 caactgcgta tcgtccaacc cgcccgtcgc ttccttgcgg tagcgggcga taaacggcac
     4381 ggtcgcgccg tcgtccaaaa gctcgacggc ggcggtgatt tgcgcggcag tcgcggagag
     4441 ttcttgggaa agaatttgag taatgttcat caatagaatt ccaacggaca ggccgtctga
     4501 aatttcagac ggcctgattt aaaaacaatc gctttaaggc agcgaattat aatattcgta
     4561 ggctttgtcc atatcttcaa actggtacat atgccctttt tccagcacca tcgcattatc
     4621 gcaatattgc ttcatggcgc tgtggctgtg cgacaccaag atgatggaac ggtctttgcg
     4681 cttttcaaac aactcgtact tacatttatc ggcaaaacgc gagtcaccaa ctgcaatcac
     4741 ttcgtcaatc aaatagcaat caaactctac cgccaacgac aaagcaaatg ccaaacgcgc
     4801 cttcatacct gaagaataac gcttgaccgg ctcgtaaaga tattgcccca actcggaaaa
     4861 ctcttcggta aagttcttca cataatcgat atcgacattg taaatccggc agatgaaacg
     4921 caaattgtcc ataccggtca gactgccttg aaacgcaccg gagaatgcca aaggccaaga
     4981 aatactcatt gtccgcttga tttcacccgt ggtcggcggc tcaacgccac tgatcaaacg
     5041 gatgagcgtc gatttacctg caccgttgcg gccgagaata ccgattttct cgcccttctc
     5101 cattttgaag ctaatatcgt gcaagactgt ccgccaacct tggcgggtca gatagcgttt
     5161 ggaaacgtgt tcaactgaaa tcattgcggc tcgactcctt tactgaattt actgaccatc
     5221 gccaagccaa acaacaacaa caccagattg cacaatacga tataccaagg attttcatag
     5281 gtaattacat cgctgccaaa atatccggca cggaacattt ctgtgccatg caccatcggg
     5341 atcatcaatg catactcttg cactttcgga ggcaaattat gtacaaaaaa gaatgcaccc
     5401 gataacggca tcatcacaaa agtcaatgtg ccccaaatct tgccaaacgg ctcgaaatta
     5461 aaggcaatcg aacaaatcac caaacccaaa ccaatcgcaa aaaaagccat caaaagccaa
     5521 gccatcagca tataaaacat atctgccggc atttcaatcc agccaatcgc aatcaatacc
     5581 gccataatca caatctgcgc aatggttgca ccagcaattt ccaaaatcat gcgcgccaag
     5641 atggtatcca aaactcttac attgcggtga taaagcaagc tggcatttga agaaatcgac
     5701 ccaactgccc gttttgaggc attacgccac atcatcaaca tcggatagcc agtaatcgca
     5761 aatgcgacaa tattcaaagt tgaatatcgg tctgccctta aaaatttcca catcaagacg
     5821 ataacgaatg tcatcagcaa cggctcaaca aacagccata aaaagccaat attattgcga
     5881 ccgtaacggg tgataatttc ccgcatcaaa agcgcaccga ttacgcgcct ttgaatggct
     5941 aaagattccc aaaatgatgt tttatgcaag gctttcatca gtttttatgc tcacgaatgc
     6001 tggcagtcaa caggctcaaa ataccataaa ccatcaagcc gataatcaga gtggcaacaa
     6061 tgttgtataa ccgtttaggc tcatgtgcca aatccggcag gctcggttgc gagatcactt
     6121 ccaaataaag ctgctgacgg tctgcttcaa ccttggcact ttccaaagaa gtcatggcgg
     6181 ctgccaactg ctgctctgcc aactggtttt ccaaatacac acgctgatac tcggcagcct
     6241 gattagacaa cgaagaatgc ccaccgcccg aaatggcacg taactgttgg tcaatttctt
     6301 tacgcaagct ctgctcacgc gcctgcaaac ccggaatctg cggattctcc ggagtgactg
     6361 ctttcacctg atccagctgg gtttgaatca caatcaattc atcttgcagc ttggaaacca
     6421 accccatttg cacttccgat tgcgctttca aatcaaaaac gccattggca atccggtaat
     6481 ccgtcagatt ctgagaggct tcctttaccc gctctgccgc cgttttcact acttcttccg
     6541 catagcgcac cgtatcagca cgtgcacgat cgttcaactg gttaatcaat gcttcacctt
     6601 gttttaacaa agcctcattg attttcttag attccagcgc atcaaaggaa gttacattca
     6661 acgtggaaat acccgaaacc gtatcaaaat tgatcatcac ctgattttta tagtattgat
     6721 aaaaagcctc ttcctcgcca cggaacccaa acccattaaa gcggctgaac gcatcacctt
     6781 tggtttcata aaactcacgc accggcaaga ttttacgcag ttcatccaaa gacgagcgcg
     6841 aacgcatata ctccccaacc gtgtaaatat catcttgcgc acgggcaaaa cctgtgccct
     6901 gcaaaatggc acccaggcca ttgagagaag attggctttt aggcgagcgc accacaaagc
     6961 tcgattgcga cgtaaaacga tcggaagcga agaagccgaa atacaccaac gaaattaccg
     7021 tagggataat caccgttacc caaaataaag ggcttagctt tttaatccaa cttttctttt
     7081 tcggcttttt acgctcggct ttggtttcag tggcaactgc cacaggaagt tgctcagaca
     7141 tcttaattac tcacattaat tagttaaatt attaatactg ttcgcgccac tggtaaccgg
     7201 cgagaacaca aacgacaaga atttctgcac ttcagccaac ggcgcattcg acacatacaa
     7261 tacatcttta ttcttcacag gaaagcgctg catagaaaat agcgaatgcg catcagccat
     7321 attcacacga tataccgttg gaatctctgc ctcactgcca taaccttgag caatccattt
     7381 atcctgacgt tctgccggca attccaccaa tggcgtatag cggaacacaa acacaccacg
     7441 cgcatcagaa cggcgatctt gcaaaccgcc catacggcca atggcttcag aaagcgataa
     7501 gcctctggct gaaaaaccga tttcttgtgt tctccccacc gcacccatag acgtaaaggt
     7561 atagggattg gtaatcatgg taaccacatc accgcgacgc agcaaaatat tttgtcgcgg
     7621 atttgcaact aaatcttcca aggcaacagt tcgtactaca ttgccacgtg tcagctgcac
     7681 attcgtatcc tgcacatttg ccgttgaacc acctaccgca gccaccgcat ccaacacacg
     7741 ctcaccggct gccgtcagcg gcatacgcac actattccca gcacgaatca ccgacacatt
     7801 cgccgcatta ttctgcacca aacgcaccat cacttgtggc tgattggcca tttttttcag
     7861 gcggccttta ataatttcct gaacctgacc aggcgtttta ccgaccaccg aaatatcgcc
     7921 aacaaacggc acagaaaccg taccacgtgc cgtgaccaac tgctctggca acttagtttg
     7981 atgcgcacta cccgagccca tcgaagaaag gccaccacca aacaatactg ccggcggcgc
     8041 ttcccaaatc ataatatcca atacatcacc aatatttagc gtaccagccg aagcataacc
     8101 atcgccaaac tgagtgaatg actgatttat ctgagcctta tataataact gagcaaccgt
     8161 atgattcaca tcaatcagct ccacttcagg aatttgaact tcagattgtt gccctaaaga
     8221 gacaattttt tttgcgctgg ggcctgatga aggaatcgca gagcatccta caattaaact
     8281 tccacacaat aataatactg cgtgacgaat ataaaatttc actttaaaca caagccaaat
     8341 cctaatataa ttataaatgg cctaattata gcacttaatc gaaataaatt tatgagtacg
     8401 tagagtataa ttagtattct tctttccaac ttccctatgc ttatagactc taaaatcatg
     8461 aaaagaattc tttgcattac aggtaccaga gccgacttca gcaagctaaa acctttatta
     8521 gcctatattg aaaatcacct agaccttgaa ttgcatttga ttgtaactgg tatgcatatg
     8581 atgaaaacat atggcagaac ctgcaaggaa gtaactcgag aaaactatca acatacatat
     8641 ctgttttcaa atcaaatcca aggtgaacca atgggtgccg ttttaggcaa taccattacg
     8701 tttatctctc gtctatctga tgaaattgaa cctgatatgg tcatgattca cggcgaccgt
     8761 ttagaagcac tagcaggcgc aactgtaggt gcattaagca gccgtttagt ttgccatatc
     8821 gaaggtggtg aactatctgg tacagtagat gactccattc gtcattctat tagtaaactt
     8881 tctcatatcc acttggtagc aaatgaacaa gctgtcactc gcctagtgca aatgggagaa
     8941 aaaagaaagc atattcacat catcggctcc cccgatttag atgttatggc ctcttccacc
     9001 ctcccatcct tagaagaagt caaagaatat tacggtttac catacgaaaa ttatggtatt
     9061 tctatgtttc accccgtgac tacagaagca catttaatgc cacaatatgc ggcccaatat
     9121 ttcaaagcat tagaattaag tggccaaaat atcattagca tctaccctaa taatgacact
     9181 ggcactgaaa gtattctgca agaattatta aaatatcaat ctgataaatt tattgcattt
     9241 ccttccattc gatttgaata ttttttagtc ttattgaaac atgctaaatt tatggtcggc
     9301 aactcaagtg caggtattag agaagctcct ctctacggtg tcccttcaat tgatgttggt
     9361 acacgccaaa gtaaccgcca tatgggaaaa tctattattc atacagatta tgaaactaaa
     9421 aatatctttg atgcgattca acaagcatgc agtttaggca aatttgaagc agatgatacc
     9481 tttaatggcg gagatactcg caccagcaca gaaagatttg ctgaagtaat caacaatcct
     9541 gaaacgtgga atgtttctgc tcaaaaacgt tttatcgatt tgaatcttta aattatggaa
     9601 aaacaaaata ttgcggttat acttgcgcgc caaaactcca aaggattgcc attaaaaaat
     9661 ctccggaaaa tgaatggcat atcattactt ggtcatacaa ttaatgctgc tatatcatca
     9721 aagtgttttg accgcataat tgtttcgact gatggcgggt taattgcaga agaagctaaa
     9781 aatttcggtg tcgaagtcgt cctacgccct gcagagctgg cctccgatac agccagctct
     9841 atttcaggtg taatacatgc tttagaaaca attggcagta attccggcac agtaacccta
     9901 ttacaaccaa ccagtccatt acgcacaggg gctcatattc gtgaagcttt ttctctattt
     9961 gatgagaaaa taaaaggatc cgttgtctct gcatgcccaa tggagcatca tccactaaaa
    10021 accctgcttc aaatcaataa tggcgaatat gcccccatgc gccatctaag cgatttggag
    10081 cagcctcgcc aacaattacc tcaggcattt aggcctaatg gtgcaattta cattaatgat
    10141 actgcttcac taattgcaaa taattgtttt tttatcgccc caaccaaact ttatattatg
    10201 tctcatcaag actctatcga tattgatact gagcttgatt tacaacaggc agaaaacatt
    10261 cttaatcaca aggaaagcta aatgcaaaac aacaacgaat ttaaaattgg taatcgttca
    10321 gtaggttaca accacgaacc attgattatc tgtgaaatcg gcatcaatca tgaaggctct
    10381 ttaaaaacag cttttgaaat ggttgatgct gcctataatg caggcgctga agttgttaaa
    10441 catcaaacac acatcgttga agacgaaatg tctgatgagg ccaaacaagt cattccaggc
    10501 aatgcagatg tctctattta tgaaattatg gaacgttgcg ccctgaatga agaagatgag
    10561 attaaattaa aagaatacgt agagagtaag ggtatgattt ttatcagtac tcctttctct
    10621 cgtgcagctg ctttacgatt acaacgtatg gatattccag catataaaat cggctctggc
    10681 gaatgtaata actacccatt aattaaactg gtggcctctt ttggtaagcc tattattctc
    10741 tctaccggca tgaattctat tgaaagcatc aaaaagtcgg tagaaattat tcgagaagca
    10801 ggggtacctt atgctttgct tcactgtacc aacatctacc caacccctta cgaagatgtt
    10861 cgattgggtg gtatgaacga tttatctgaa gcctttccag acgcaatcat tggcctgtct
    10921 gaccatacct tagataacta tgcttgctta ggagcagtag ctttaggcgg ttcgatttta
    10981 gagcgtcact ttactgaccg catggatcgc ccaggtccgg atattgtatg ctctatgaat
    11041 ccggatactt ttaaagagct caagcaaggc gctcatgctt taaaattggc acgcggcggc
    11101 aaaaaagaca cgattatcgc gggagaaaag ccaactaaag atttcgcctt tgcatctgtc
    11161 gtagcagata aagacattaa aaaaggagaa ctgttgtccg gagataacct atgggttaaa
    11221 cgcccaggca atggagactt cagcgtcaac gaatatgaaa cattatttgg taaggtcgct
    11281 gcttgcaata ttcgcaaagg tgctcaaatc aaaaaaactg atattgaata atgcttatta
    11341 acttagttac tttattaaca gaggattggc tattacatat agctaattct cattaatttt
    11401 taagagatac aataatgcta aagaaaataa aaaaagctct ttttcagcct aaaaagtttt
    11461 ttcaagattc aatgtggttg acaacatctc cattttatct taccccccca cgtaacaatt
    11521 tatttgtcat atctaattta ggtcagctta accaagtcca aagcctaatt aaaatacaaa
    11581 aattaaccaa taatttacta gtaattttat atacttctaa aaacttaaaa atgcctaagt
    11641 tagttcatca atcagctaac aagaatctat ttgaatctat ttatctattt gagcttccta
    11701 gaagccctaa taatataact cctaaaaaat tactttatat ttatagaagt tacaaaaaaa
    11761 tccttaatat tatacagcct gctcatctct atatgctgtc ttttacaggc cactactcct
    11821 atctgattag tattgcaaaa aaaaagaata ttacgactca tttaattgat gaagggactg
    11881 gaacatatgc tcctttatta gaatcatttt catatcatcc aacaaaatta gaacgttatt
    11941 tgattggaaa taatcttaat attaaaggat atatagatca ttttgacata ttgcatgtcc
    12001 cctttcctga atatgctaaa aaaatattta atgcaaaaaa atataaccgg ttttttgcgc
    12061 atgctggagg aataagcatt aataataaca ttgcaaactt acagaaaaaa tatcaaatat
    12121 ctaaaaatga ctatattttt gttagtcaac gctaccccat ttcagatgat ctgtattata
    12181 agagtatagt agaaatctta aacagcataa gtttacaaat taaaggaaag atatttatta
    12241 aactacaccc aaaagagatg ggcaacaact atgtaatgtc tttatttcta aatatggtag
    12301 aaataaaccc tcggctggta gttattaatg aacctccttt tctaattgag cccctaatat
    12361 acttaacaaa tcctaaagga attataggcc tggcctctag ttctttaatt tatacaccat
    12421 tactctcacc ctcaacccaa tgtctttcta ttggagagtt aattattaac ttaattcaaa
    12481 aatattcaat ggtggaaaac actgaaatga tccaagaaca cttagagatt attaagaaat
    12541 ttaattttat taatatacta aatgatttaa atggggtaat aagtaaccct ctctttaaaa
    12601 cagaagaaac atttgaaaca cttcttaaat ctgcagaatt cgcatataaa tctaaaaact
    12661 actttcaggc tattttttac tggcaacttg ccagcaaaaa caatattacc ttattagggc
    12721 ataaagcatt atggtactac aatgcacttt ataatgtaaa acaaatttat aagatggaat
    12781 attcagatat tttttatatc gataatatct ccgtagactt tcatagtaaa gataaattga
    12841 catgggaaaa aattaaacat tattactatt ccgccgacaa tagaattggt agagatagat
    12901 aaaaatacta tgacttctga tctccatacg tatgcttgtt ttcaaaaagc agcattaaga
    12961 tagataagtg aagcttgatg agagccattt ttgtggtcat cgccaaggta cacgcggaga
    13021 gtaatcgaca acagagctgc tgtattctgg cttttaaagc ccagtagtaa agtttataag
    13081 gctgcatttc tcaatgtaaa acgcgatatt ttcacccatt attagggata agtagccagt
    13141 ggtttacact ggctactttc atagttatga agtactgggt ctgagtgaat tcaattattt
    13201 ccgtataaaa cacagtagac attttactaa tcggcaaaat tatattagaa gaattgagaa
    13261 ctactgaagt caggtaagat accattttgc acaaatttaa cggcatccct aaaaagcatt
    13321 ttgggtggta tttaaaggag tacaaaatgg ttttaactac agtatgctta gaaatctata
    13381 gccttacgcg ctttatatta aatcaccttt ctcaaccatt tagaatcata aaaaaacttt
    13441 ttaggatccc taattaactt tttaaatttt cttaaaaaaa tagttttagt tgattctata
    13501 ctattcaccg cagataaata ttcttggtaa tcatgttgca atggcacaac ctgctttaat
    13561 agcttattaa actcatcagt tgtaatcacc tttttagtag catagcaatc aagccaatcc
    13621 ggatctgcta tttttgggga agtgaaatct atttgatcaa aataaactaa aaccccacta
    13681 cctaaaaaat gctgatttgc ataggattta gaaaagccta agtctgcaac aacaccaaca
    13741 gggataccct tacattcagc ttcaaaaagc atagtagatg aataagataa acaatacccc
    13801 atttcttgga aggcttgcgc ggtacttttt cgctctattg tcaaattact cggcaactga
    13861 aactgctttg ccagctctat atacgaatgt ttatcctgat gcacagtaat atctttatct
    13921 gcaaccctta gcaaaatagt aaattctttc tcagggtatt tttcagccaa ggcaatcaat
    13981 ttttttaatg tataaattct ttcttctttt ttaaatggga ttttaacttg gtcaataaag
    14041 taaattttct ctccatgacg accttttgat gcgtggcgaa aaattggata accataaagt
    14101 atattttgac aacttaattt atattgtttc ttatattcct ctgcaattct aaaatcatgc
    14161 ttattattat ataaaacaat atcagccccc atacgagata gaatacttgc ctgatcaccg
    14221 aatactatac ctggaaacaa ggtaataatc aatggccttg aagcaatatt taattgcgca
    14281 ttctgcttaa agaacctttt taacaaccca tttccaaccg ataaaattac tgcgtcataa
    14341 tattgatgca tattcttaat aaagtaatca tcaatatgaa aaaaaattgc ttggtcttta
    14401 tctatccctg attctaataa ctgtcgattt gaaagaatgt tttctcgact tttgtggata
    14461 taaatatcaa tttgagcatc ttttatctct tttgcaacag catagcccga gttaaggaac
    14521 gagtcataac tggcaattag taatactttt ttcactattt tcccttaatt ctacaccata
    14581 tgaataaaat tatagtattt taccttagat atcaaatata cttaaccaaa aaaatctgaa
    14641 gaaatctact gctacttcag ggataattat ttcattaaga ttttacaacc attattatca
    14701 atctatcaaa gtatttttag tattttattc tatgaaaaaa attctcgtta ccggcggcac
    14761 cggttttatc ggctcgcata ccgttgtttc tttgctgaaa agcggccatc aagtcgtgat
    14821 tttggataac ctatgcaatt ccagcatcaa tatcctgcca cgcttgaaaa cgattaccgg
    14881 ccaagagatt ccgttttatc aaggcgatat ccgcgatcgt gagattttgc gccgtatttt
    14941 tgcggaaaac cgcattgatt cggtgattca ttttgccggc ttgaaagcgg tgggtgaaag
    15001 tgtggccgag ccgatgaaat attatgataa taatgtttcc ggcagcttgg tgttggcgga
    15061 agaaatggcg cgtgcgggcg tgtttagcat tgtgttcagt tcttcggcga cggtttatgg
    15121 cgatccgggc aaagtgcctt ataccgagga tatgccactg ggcgatacca ccagccctta
    15181 cggcgcatcg aaatcaatgg ttgagcgcat tctcactgat attcaaaaag ccgatccgcg
    15241 ctggagcgtg attttgttgc gttatttcaa tccgattggc gcgcacgaaa gtggtttgat
    15301 tggcgagcag ccaaacggca tcccgaataa tttgttgcct tatatctgcc aagtggcggc
    15361 aggcaaactg ccgcaattgg cggtatttgg cgatgactac cctacccccg acggcacggg
    15421 gatgcgtgac tatattcatg tgatggattt ggcagaaggc catgtcgcgg ctatgcaggc
    15481 aaaaggcggc gttgccggcg tacatttgtt caacttgggt tcggggcgcg cctattccgt
    15541 tttggaaatc atccgtgcct ttgaagccgc atccggctta aatattccct atcaaatcca
    15601 accccgacgc gccggcgacc tcgcttgttc ctttgccgac ccgtcccata ccaaacaaga
    15661 gacaggctgg gaaaccaaac gcagccttgc acaaatgatg gaagattcgt ggcgttgggt
    15721 cagccgcaac cccgacggat acggggatta aatgcaaacc gcaaataaaa aaaccatcct
//
LOCUS       DAWHRA010000035        15772 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWHRA010000035 DAWHRA010000000
VERSION     DAWHRA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.035"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..482
                     /locus_tag="WIB30_000829"
     CDS             <1..482
                     /locus_tag="WIB30_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T cell stimulation protein TspA"
                     /protein_id="HEZ3796923.1"
                     /translation="ETPDFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAE
                     APAVEENATEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTGFP
                     SESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(655..1131)
                     /gene="gloA"
                     /locus_tag="WIB30_000830"
     CDS             complement(655..1131)
                     /gene="gloA"
                     /locus_tag="WIB30_000830"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ3796924.1"
                     /translation="MADVYAKQNRSLTTTERNTKMRLLHTMLRVGNLEKSLDFYQNVL
                     GMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDA
                     YEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(1095..2264)
                     /gene="lapB"
                     /locus_tag="WIB30_000831"
     CDS             complement(1095..2264)
                     /gene="lapB"
                     /locus_tag="WIB30_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ3796925.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2275..2589)
                     /locus_tag="WIB30_000832"
     CDS             complement(2275..2589)
                     /locus_tag="WIB30_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ3796926.1"
                     /translation="MKLIYTVVKIIILLFFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            2899..3897
                     /gene="ilvE"
                     /locus_tag="WIB30_000833"
     CDS             2899..3897
                     /gene="ilvE"
                     /locus_tag="WIB30_000833"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ3796927.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVAELKAAVENGAEA
                     ILTGTAAVISPVTSFVIDGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(4141..4926)
                     /gene="fabI"
                     /locus_tag="WIB30_000834"
     CDS             complement(4141..4926)
                     /gene="fabI"
                     /locus_tag="WIB30_000834"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ3796928.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGADLAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(5081..5902)
                     /gene="dapD"
                     /locus_tag="WIB30_000835"
     CDS             complement(5081..5902)
                     /gene="dapD"
                     /locus_tag="WIB30_000835"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ3796929.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(6073..7716)
                     /gene="pgi"
                     /locus_tag="WIB30_000836"
     CDS             complement(6073..7716)
                     /gene="pgi"
                     /locus_tag="WIB30_000836"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ3796930.1"
                     /translation="MDAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDEVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETARHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGVGAA"
     gene            8226..9458
                     /gene="pilG"
                     /locus_tag="WIB30_000837"
     CDS             8226..9458
                     /gene="pilG"
                     /locus_tag="WIB30_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ3796931.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9532..10392
                     /locus_tag="WIB30_000838"
     CDS             9532..10392
                     /locus_tag="WIB30_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ3796932.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKKHLNLPLTEEESRTFNLMKPDSCCPKCRVPIRARQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10394..11026
                     /locus_tag="WIB30_000839"
     CDS             10394..11026
                     /locus_tag="WIB30_000839"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ3796933.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            11019..11228
                     /locus_tag="WIB30_000840"
     CDS             11019..11228
                     /locus_tag="WIB30_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ3796934.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(11313..12989)
                     /gene="pilB"
                     /locus_tag="WIB30_000841"
     CDS             complement(11313..12989)
                     /gene="pilB"
                     /locus_tag="WIB30_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ3796935.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(13130..13192)
                     /locus_tag="WIB30_000842"
                     /pseudo
     CDS             complement(13130..13192)
                     /locus_tag="WIB30_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13389..13460)
                     /locus_tag="WIB30_000843"
     tRNA            complement(13389..13460)
                     /locus_tag="WIB30_000843"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13425..13427),aa:Arg,seq:cct)
     gene            complement(13480..13926)
                     /locus_tag="WIB30_000844"
     CDS             complement(13480..13926)
                     /locus_tag="WIB30_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ3796936.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(13930..14904)
                     /gene="ispB"
                     /locus_tag="WIB30_000845"
     CDS             complement(13930..14904)
                     /gene="ispB"
                     /locus_tag="WIB30_000845"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ3796937.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(14920..15108)
                     /locus_tag="WIB30_000846"
     CDS             complement(14920..15108)
                     /locus_tag="WIB30_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796938.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            15128..15436
                     /gene="rplU"
                     /locus_tag="WIB30_000847"
     CDS             15128..15436
                     /gene="rplU"
                     /locus_tag="WIB30_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ3796939.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            15461..15733
                     /gene="rpmA"
                     /locus_tag="WIB30_000848"
     CDS             15461..15733
                     /gene="rpmA"
                     /locus_tag="WIB30_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ3796940.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT         3786 a         4349 c         4020 g         3617 t
ORIGIN      
        1 ccgaaacacc cgatttcaac gccaccgcag acgatttgtc cgcattactt caaccttctg
       61 aagtacctgc cgttgaggaa aatgcagcgg aaaccgttgc cgatgatttg tccgcactgt
      121 tgcaacctgc tgaagcaccg gccgttgagg aaaatgcaac ggaaatcact ttggaaacgc
      181 ctgattccaa cacctctgag gcagacgctt tgcccgactt cctgaaagac ggcgaggagg
      241 aaacggtaga ttggagcatc tacctctcgg aagaaaatat cccaaataat gcagataccg
      301 gtttcccttc ggaatctgta ggttctgacg cgccttccga agcgaaatac gaccttgccg
      361 aaatgtatct cgaaatcggc gaccgcgatg ccgctgccga gacagtgcag aaattgctgg
      421 aagaagcgga aggcgacgta ctcaaacgtg cccaagcatt ggcgcaggaa ttgggtattt
      481 gattcccaac tgccctttcg cagatcaagg atgccgtttc agacggcatc tttttttgcc
      541 ttatcggtgt aacggataaa gtttgaaccg gcacaggctc aaacagcagg tcgacggcaa
      601 caaaatgccg tctgaaaccc ctaaaggctt cagacggcat tggcggcggc ggtatcaggc
      661 agtttgatag gcaaccgaat cgtcaccgct tttcttttga atgaactcga ttttgtatcc
      721 gtcggggtct tcgacgaagg ctatcacggt tgtgccgtgt ttcatcgggc cggcttcgcg
      781 gacgacgttt ccgccctgcc gcttcacacg ttcacaggct tcgtaggcat cgtccacttc
      841 aaccgcgatg tgtccgtagg cgttgcccaa gtcgtatcgt tccgtatccc agttgtgcgt
      901 cagttccaaa accgtgctgt cggtttcgtc gccgtaaccg acgaaggcaa gggtaaatct
      961 gccttcggga taatcttttc ggcggagcag tttcataccc aaaacgtttt ggtagaaatc
     1021 gagggatttt tcgagattgc ccacgcggag catagtatgg agtaagcgca tttttgtgtt
     1081 cctctcggta gtggttaaac ttcgattttg tttggcgtaa acgtctgcca tttgttgcag
     1141 gcaggacaat gccagaaaaa gacttgtgat ttgaagtggc agtttcggca ccggtacatc
     1201 acgctgcgct gtagctgccg tccgataacc gaacgcatca tatcggcatc ggctttccaa
     1261 gccggatcca aatcgctgag tttcaaacca agcaggcggt acacgccgtt gaggtcgggc
     1321 ttgcggcgga caagctcgac ggcggtttgc gcggcttctt tctcgcactt aagcagcagg
     1381 gatttctcgt acacgacatt gatcaggtca agttcgggaa acgtctgcat atatcctgtc
     1441 agacggttca agccttcttc aggttttccc tgcgcggcat aggcttcgta aagcttctcg
     1501 ccgaccatgc tcaagtatgc atggttttgc tgctcgatgg cggcataggc ttcgacggcg
     1561 gcagggaaat tgccttgtcg gtgttcgatg tcgcccaaaa tcatgttggc acgggtgcat
     1621 tttttgttgg cttcgagtgc cttgccgaca ttgaaacgcg cgacatcgaa attggacttg
     1681 aacagcgcgg cttgggcaag ttcgcaataa aactgggcga tttcaaactg ataggtctga
     1741 tcgtcatggc tgagcagccg ggcggtttca accgcttttt cccaatccct gtcctgttgg
     1801 tagatattga gcaggtgctg tctggcttca cgcgccattt taccgtcttg cagccccaaa
     1861 aaaatctgtt cggcacgatc gaccaacccc gcactttggt agttttgcgc caattcaaac
     1921 aggacgcgcg cgcgcttggc tccggttgta tcgggagagt caagcaatgt ttggtgcata
     1981 ttgatggctt tgtcgttttc gccacgctgg cggtaaagtt tgccgagggt gaggttcaaa
     2041 tcatacgatt gcggccggcc gtcgacgact tccgccaact cccttgccgc gcgcccgctg
     2101 ttgcggtcga ccaaagcgtc caagctttta taaaatcccg aagggatgct ttttgcctgc
     2161 ttcaatacgg ttttcatatc cacgcgggcg gcaaaccagc ccatcgcgaa gaagacgggc
     2221 aaaaggataa tcggcagcag gataatccac aattcgttgt ccatatcggc tttcttaagg
     2281 ctgtttggta gattcgggcg cattttgcgc cggtggtgcg gtcagctcct tccccgtcaa
     2341 acgcgcattt ttctttactt cggcacgcaa cctgccgttc tcgccacgta acgacaacaa
     2401 ccgtccgaac aaggcaaaca ttccaaaaat aataccgact acaaatgcgc cgaacaatac
     2461 gacaatcagc ggcaaatcga atttttgccc cggcaggtag gagaaggtaa cggcatccgt
     2521 attaatgacg gcaagcagca ggaagaacag caggataatg attttgacga cggtatagat
     2581 aagtttcatg tttgctccgt aaataaaaac gccgcccgga cagcttcgca ctctgaacca
     2641 agattgcgct agtttaaacg atttgcacgg tttcggatag gatgcggcag cgtgttcgga
     2701 cgacatacgg agtatgcgat tgccgacaat tttaccaaat acaccgttcc tttccatttg
     2761 aaaaataacg gattggacac cgcatcgaca gaaaaacccg ccgcgcactt gtcaaaaccc
     2821 tgtttgcagg cgtatcttta caatcttcaa attcaaaccg ttcattgaaa catatcagaa
     2881 taagaaaggc tttacatcat gagcagaccc gtacccgccg tattcggcag cgtttttcac
     2941 agtcaaatgc ccgtcctcgc ctaccgcgaa ggcaaatggc agccgaccga atggcaatct
     3001 tcccaagacc tctccctcgc accgggcgcg cacgccctgc actacggcag cgaatgtttc
     3061 gagggactga aagccttccg tcaggcagac ggtaaaatcg tactgttccg tccgactgcc
     3121 aatatcgcgc gtatgcggca aagtgcggac attttgcacc tgccccgccc cgaaacccaa
     3181 gcctatctcg atgctttggt cgaactggtc aaacgtgccg ccgacgaaat tcccgatgcg
     3241 cctgccgccc tgtacctgcg cccgacctta atcggtaccg atcccgttat cggcaaggcc
     3301 ggttctcctt ccgaaaccgc cctgctgtat attttggctt cccccgtcgg cgactatttc
     3361 aaagtcggct cgcccgtcaa aattttagtg gaaaccgaac acatccgctg cgccccgcat
     3421 atgggccgcg tcaaatgcgg cggcaactac gcttccgcca tgcactgggt gctgaaggcg
     3481 aaagccgaat acggcgcaaa ccaagtcctc ttctgcccga acggcgacgt gcaggaaacc
     3541 ggcgcgtcca actttatcct gattaacggc gatgaaatca ttaccaaacc gctgaccgac
     3601 gagtttttgc acggcgttac ccgcgattcc gtgctgacgg ttgccaaaga tttgggctat
     3661 accgtcagcg aacgcaattt cacggttgcc gaactgaaag ctgcggtgga aaacggtgcg
     3721 gaggcgattt tgaccggtac ggccgcggtc atttctccgg taacttcttt cgtgattgac
     3781 ggcaaagaaa tcgaagtgaa aagccaagaa cgcggctacg ccatccgtaa ggcgattacc
     3841 gacatccagt atggtttggc ggaagacaaa tacggctggc tggttgaagt gtgctgatgc
     3901 tttaaataaa aaatgccgtc tgaaacccgt ttggcgtttc agacggcatt ttcgcatccg
     3961 aaccgtttcc gctgcacctg cagcaagtcg gcacaaaggc aatcggttaa aacaagcgtc
     4021 cgcatttccc atcccgcctg ccgtaagtcg ggcatttccc tagaaatacg cttcagacgg
     4081 caaaacgccg cccgaaaccg atatgcggca cggacggcgc acggatttga aaacggcgga
     4141 ttatccctcg gtgctcaagg cattgatgct gtaaccgccg tcaacgtaag tgatttcgcc
     4201 ggtaatgccg gacgacaggt cggacagcag gaaggcggcg gtattgccga cttcttcgat
     4261 ggtaacgttg cggcgcagcg gattgtgggc ggcgacgtgt cccaagagtt tgccgaaatc
     4321 ggcgatgccg gaggcggcga gtgttttgat cggaccggcg gaaatgccgt tgcagcggat
     4381 gccttctttg cccaaacagg cggcggtaaa gcgaatgccc gcctcaaggc tggcttttgc
     4441 catacccatc acgttgtagt tcggaatcgc gcgcaccgcg cccaagtagc tcagggcaac
     4501 gatggcggag tttctgccgc gcatcatcgg acgggcggct tttgccaatg cgggcaggct
     4561 gtatgcggaa atttcgtgtg cggtgttgaa cgcttcgcgg ctgatgctgt cgaggaaatc
     4621 gccgctcaag gcttctttcg gtgcgaagcc gatggaatgc accaaaccgt ccaagccgtc
     4681 ccaatgtttg cccaagtcgg cgaacacttg gttgatttcg tcgtcactgg cgacatcgca
     4741 gcggaatacg agttcggaat ccaattccgc cgccattttg cggacgcgct cttccagttt
     4801 gtccacaacg taggtaaacg ccaaatccgc gccttgttcg cggcaggctt tggcgatgcc
     4861 gtaagcgatg gaacgctcgg aaatcatgcc ggtaatcaga atttttttgc cttgcagaaa
     4921 acccattttg tatccttcaa atagtgttcg gttcgttcta aaccgttgat tatagcaaat
     4981 tgtccctgtt tctgtgtttt cacgttgcag cgtgcaaacg gcaatgccgt ctgaagcgga
     5041 tttcagacgg catcggacgt ttcaaatacg gtttaaggca tcagatgccg cgcaacaatt
     5101 cgttgacgct ggttttcgca cgggtttgcg cgtccacgcg tttgacgatg acggcgcagt
     5161 aaaggctgtg gctgccgtct ttggaaggca tactgccgga tacgacaacc gaacctgccg
     5221 gtacgcggcc ttggtagatt tcgccggttg tacggtcaaa gattttggtg gattgaccga
     5281 tgaacacgcc catagaaatc acgctgcctt cttcgacaat cacgccctca acgatttcag
     5341 aacgcgcacc gatgaagcag ttgtcttcaa taatggtcgg gctggcctgc aggggttcga
     5401 gtacgccgcc gatgccgaca ccgccgctca agtggacgtt tttaccgatt tgcgcgcaag
     5461 agccgacggt cgcccaagta tcgaccatcg caccttcgtc aacgtatgca ccgatgttga
     5521 cgtaagacgg catcagcacg acatttttcg ccacaaagct gccgcgtcgg gcaaccgcac
     5581 ccggaactgc gcggaagcct gcgtttttga actcgtcttc agaccaatcg gcaaacttgg
     5641 tcggcacttt gtcgaagtat ttgttcacgc cgtcgttgag gacttcgttg tcttggatgc
     5701 ggaaggacag caacacggct tttttcgccc attcgttgac tttccattcg cccacgccca
     5761 agcgttcggc aacgcgcagt ttgcctgaat cgagctggcg gatggtttcc aacacggctt
     5821 ctttaacttc tggggcaacg gtggtcgggg tgatgtccgc gcggttttca aaggcggttt
     5881 cgataatgtt ttgcaaagac ataatgtttc cttatgtgag atgtttcgtg ggacgcttgc
     5941 cggtattttc aggcagtatg ttcatttgaa atgaatgcgc tattgtgcca cgcaaacgca
     6001 tattcgtcag caatacgcgg gcggttgccg aacattggcg tttcggaatc gggcgggcgg
     6061 caggcattgc ctttatgccg cgccgacgcc tttcagacgg cattcacggt aaaacgccat
     6121 cagcccttcg gtcgaggcat cgtgtacgga cgtgccgcct tccagttcgc cgatgatggt
     6181 tttcgccaac tgtttgccgt attccacccc ccactgatcg aaggggttga cgttccatat
     6241 cgcgccttgg acgaaggttt tgtgttcgta agccgccatc agcataccca gattgtaggg
     6301 cgtgaggcgg tcgagcagga tgctgttgct ggggcggttg ccggggaatt ctttgtgcgg
     6361 cgcgaggcgt tcgcgttccg cttcgggcaa atctgccagt tcggcgcgtg cttcgtccaa
     6421 ggttttgccc ttcatcaggg cttccgcttg ggcaaaggcg ttggcaacgg taaaacggct
     6481 gcgtccgtcc tccacgccct gcgccgtcat cgggacgata aagtcgcagg gaatcaggcg
     6541 cgtgccttga tgaagcagtt ggaaataggc gtgctggcag ttgacccctt caccgccgaa
     6601 cacgatgccg cccgttttgc acacggcggg actgccgtct gaagcgcggc ttttgcccaa
     6661 actctccata tcgagctggt tcagccacgc cggcagcagg cgcaggttgt ggctgtacgg
     6721 aacggcggtc tgcccgtccg cgtgttggaa attgttatac cacacggcaa tcagtgccat
     6781 cagaacggga atgttgcggc gcggcggcgt gtggaaaaaa tggctgtcca tcgcgtgcgc
     6841 ccccgccaac aactcgcgga aacgcgcccc gccgaccgca accatcacgg gcaaaccgac
     6901 gggcgaccag acggaatagc gtccgcccac ccagtcgtac atcgcaaaca cgcgttccgc
     6961 cgcgataccg aaagccgcag ctgccgctgt gtcggccgat accgcgcaaa aatggcgtgc
     7021 tgtttcggat tccgagaacc ccgcaccacg ataccaagcc ttgaccgcct gtgcattgag
     7081 cagggtttca ggcgttttga aggacttgct ggcaacgcaa aacaccgtcg tttcggggtt
     7141 cagacggcat aagacctcat ccaggcaggc aggatcggcg ttggcggcaa aatggacggc
     7201 gatatgccgt ctgaacggct cgagtgcctg cacgcacatt gccggcccga ggtcggatcc
     7261 gcctatgccg atgtggacaa aatccgtaat ccgttttccg gttgtcccct gatacgaacc
     7321 gtcgtccaaa ctgtgtgcaa acttcaacgc acgatttaac tcgcggcgga tttcgggcaa
     7381 cacgtccctg ccgtccgcat aaacggcacc cgcaccgtcg ggcaggcgca aagccgtatg
     7441 cagcgcggca cgcccctcgc tgccgttgac tttcgcaccc gtccgcaaag cacgcatttt
     7501 cccttccaaa tccgccgcat cggcaagatt gcagagcagt tgcagcgtat cttcgcccaa
     7561 acggtttttg ctgtaatcga acaacatccc gtccaaacgc tcgtgcatac gctcaaaacg
     7621 gtcgggttcg caggcaaagc ggtcgcgcaa aaggacatga cgcgtatcct gataatggcg
     7681 ttcgagcgca taccatgcac gggtaaaagc atccatctgt tttccttgat ttttcagaac
     7741 cggattaaaa tgtagcagaa tgtagtttaa caaacggcag cggctttggc gaatctccgg
     7801 aacaccgccc gccgcgtcca atagcacctt atttccatat ggcgaaaata tagtgaatta
     7861 acaaaaatca ggacaaggcg gcgagccgca aacagtacaa atagtacgga accgattcac
     7921 ttggtgcttc agcaccttag agaatcgttc tctcttgagc taaggcgagc caacgctgta
     7981 ccggtttttg ttaattcact ataccctccg gccggcattt gacccgcaac aatatcctgc
     8041 aagatttatt gtgtacgcat aaatgccgga cagccgccta aataaggtat aatcgcatcc
     8101 gattctgtcc cgtttgccgc ccgcaggcgg gatgtcggcg gttttcatct taccgggaac
     8161 acgccggtac tgttttttaa cgatgtttct tacattttat tccaattact ttacggggct
     8221 agaatatggc taaaaacgga ggattttctt tgttcgcaaa gaaagaaaaa cgctttatct
     8281 ttgaaggcag gcattccgcc tccgacaaac tggtcaacgg cgaagtatcc gcgtttaccg
     8341 aagaagaggc gcgcaaaaaa ctggcaaaac gcggcatccg cccgttgcag attacccgtg
     8401 tgaaaacaag ctccaagcgc aaaatcacac aagaagacat caccgttttc acccgccagc
     8461 tgtccacgat gattaaagcg ggcctgccgc tgatgcaggc atttgaaatc gtggcgcgcg
     8521 gacacggcaa cccgtctatg acggaaatgc tgatggaaat ccgaggcgaa gtggaacagg
     8581 gcagctcgtt gagccgcgca ttctcaaacc acccgaaata tttcgaccgc ttctactgca
     8641 acctggttgc ggcgggcgaa acgggcggcg tattggaaag cctgctggac aaattggcaa
     8701 tttacaaaga aaaaacccag gccatccgca aaaaggtaaa aaccgcgctg acctatccgg
     8761 tatccgtcat cgccgtcgcc atcggtttgg tattcgtgat gatgattttc gtactgcccg
     8821 cctttaaaga agtttacgcc aatatgggcg cggagcttcc cgcactgacc caaacagtga
     8881 tggatatgtc cgactttttc gtctcatacg gctggatggt gctgatcgca ctgggctttg
     8941 ccatatacgg cttccttaaa ttgaaggcgc gttcgattaa aatccaacgg cgtatggatg
     9001 ccatactgct gcgtatgccg attttcggag acattgtccg caaagggacg attgcccgct
     9061 ggggcaggac gacagcgacg ctgattgcgg caggcgtgcc tttggtcgat gtattggact
     9121 ccactgccgg cgcggcgggc aatttaatct atgaagaagc cacccgggaa atccgtacgc
     9181 gcgtcatcca aggtttgtct atgacttcgg ggatgcgtgc gacggaactg ttccccaata
     9241 tgatgttgca gatgtcttcc atcggcgagg aatcgggttc tttggacgat atgctcaaca
     9301 aagccgccga attttacgaa gacgaggtgg acaatgcggt cggcaggctg tccgctatga
     9361 tggagccgat cattatcgtg attttaggct tggtcatcgg cacgcttctg gtcgccatgt
     9421 atctgccgct gtttaatttg ggcaacgtgg tcgcctgatt tgccgcacag atccggcgcg
     9481 gattcgctct gcgccggttt gtttttgttt tgaatacatt gagaacaaaa tatgtctgat
     9541 ttgtctgtat tgtcgccgtt tgccgtgcct ttggcagcgg tgttcgggct gctggtcggc
     9601 agtttcttaa acgtcgtcat ttaccgcgtg ccggtcatga tggaacgcgg ctggacggta
     9661 tttgccaaaa aacatttaaa cctgccgctg accgaagagg aaagccgtac cttcaacctg
     9721 atgaaaccgg attcctgctg tcccaaatgt cgcgtgccga tacgcgcgcg gcagaacatc
     9781 ccgattgtca gctacctgct cctgcgcggc aaatgcgcct cctgccaaac caaaatcagc
     9841 atacgctatc ccttaatcga actgctgacc ggcgtattgt tcgggctggt tgcctggcaa
     9901 tacggctggt cttggattac attgggcggt ttgatactga ccgcgtttct gatttccctg
     9961 acctttatcg atgcggacac ccaatacctg cccgactcga tgacattacc cttgatttgg
    10021 ctggggctga tatttaattt ggacggcggc ttcgtgcctt tgcagtctgc cgttttaggt
    10081 gcggttgccg gctatggttc gatatggctt ttatgcgcgg tatataaact gattaaaggc
    10141 gaaatcggta tgggcaacgg agatttcaaa ctgattgccg cattgggcgc gtggctcggc
    10201 atatccgcat tgcccgtgct gatttttgtt tcctcgctga tcggtttggt cgcggcaatc
    10261 gttatgcgcg tcgccaaggg gcagcatttt gccttcggcc ccgcactgac agtttcgggc
    10321 tggataattt ttacggcaaa cgattccgta tggcgggcgg tcaactggtg gctgacccat
    10381 ccggtgctgt aagatgacgg tatgggtcgg actgaccggc ggaatcggca gcggcaaatc
    10441 ggcagccgcg caatgttttg ccgatttggg cgtgccgcgc atcgatgcgg acgcggcggc
    10501 gcactcgctg acggcttcag acggcatcgc cctgccggaa atcaggcggc tgttcggcga
    10561 caccgttttc gacacacagg gtttgttgcg gcgcgacata ttgcgtaaag aagtctttgc
    10621 ctccccatcg cgaaaagcct tgctcgaatc cgtgatgttg ccgctgattt tctcagaaat
    10681 caaaaaacag caagaaacct ttaccgatgc ggcttacggc attgtcgaaa ttccgctgct
    10741 gacggaaaag cgtcaattta tcagcctgat acggcgtgtc ctgaccataa gcgcaccttt
    10801 ggaaaaacgt atcggcaggg tgatggcccg cagcgggctg acgcgcggcg aggtggctgc
    10861 cgtcatcagc catcaggcat ccgaatccga acgcctgctg cttgcagacg atgtgctgct
    10921 caatgacggc agcctcaaaa gcctgcgtga gaaaacaatg cgcctgcacg cgttttattc
    10981 agggattttc gcctcaaaac caacacaagg aaaacacaat ggctgaatcg cggcaaacac
    11041 gccttcaagt caaatgtccg acctgtcaaa cagcagtagt atgggaaccc gaaaacgcat
    11101 tccgcccctt ctgttcgcaa cgctgcaaac tgatcgactt gggcggatgg gcagacggga
    11161 aatatacggt ttccggccaa acggaaagtt tgccggaaat atccgaaccc gacatggcat
    11221 accgctgacc gccccgcctt cccggcaaac accctgaaag tcaaatgccg tctgaaacaa
    11281 acacgcttca gacggcattt tcattctcaa acctaatcgt tggtatttgc cgttacctct
    11341 tccaatgaag taatgccctg cataactttc aaaataccgg cccggcgcaa atccaccata
    11401 ccctccttat aggcaacgtc caaaatatcc acttccgtac cgttgttcat aatcacacgc
    11461 tgcatttctt cgctgatggg cataacctca tacacgcccg cacgcccctt ataaccctgc
    11521 ccccggcaac ggtcgcaacc gacggcgcgg taaagtttcc aatcttttgc aaggtcctca
    11581 tcggtgaagc cgacttcctt caaagcagag gcagacgggc gttccacttc ctgtttgcag
    11641 ctcgaacaca gcctgcgtaa aagacgctgc gccataatca ggctgaccga actggcaata
    11701 ttaaacggcg cgacacccat attcagcata cgcgacaacg tcgccggcgc attattggtg
    11761 tgcagggtgg aaaacaccat atgccctgtt tgtgccgcct taatcgcaat atcggcagtt
    11821 tccaaatcac gaatctcacc gaccataatg atgtccgggt cctgacgcag gaaagacttc
    11881 aaagcagcgg caaaagtcag accctgctta tcattgacgt taacctgatt gatgcccggc
    11941 aggttaatct cggcagggtc ttccgccgtt gcaatattta ccgactccgt attcaaaata
    12001 ttcaaacagg tatagagcga caccgtctta cccgaacccg tcggaccggt taccagcacc
    12061 atcccgtagg gacggtgaat cgcttccaac aacaattttt tctgaaacgg ctcaaaaccg
    12121 agctggtcaa tgttcaaaga cgcggcatcg gaattcaaaa tccgcatcac gaccttttcg
    12181 ccaaacagcg tcggcaatgt gctgacacgg aaatcgacag gcttgccgcc cttttgaaag
    12241 gtcagctgca tcctgccgtc ctgcggtatc cgtttttcgg aaatgtccaa acgcgacatt
    12301 accttaatcc gggaagcaag ctgccccctt accgcaatgg gcggctgaac cacctcgcgg
    12361 agctgcccgt ccacacggaa acggatacgg gcattgtgtt cgtaaaactc gaaatggatg
    12421 tcggatgccc cgctgcgcaa ggcatccgac aaagtcttat ggataaacct cggaacaggg
    12481 ccgtcttctg cctcctcgtt gtcgatatac agggtgtggc tttcctcttc ctcctgcccc
    12541 tccccaagct cctgaagcag cgatgtcgaa cgcgaaccca cccaatcgag caaacccgcc
    12601 aactggtcat cctcgacaat gaccaattca acctcaatcc ctgcggcaga aacggttttc
    12661 tgaatttgcg gcatctgtgt cggatcggaa accgcaaaaa atactttgtc gccccgacgg
    12721 aaaaccggca cacagtggaa ctccaccatc tgctcctccg tcaacacccc catcagcacc
    12781 ctgtggcgcg gataatgacg caaatcaaga atcgaataac tgaacaccct cgcaatcaat
    12841 gccgcaagcg acttgggcga aatgacaccg tctgaaaaca gcatcggcaa cacttcctta
    12901 cccgcctgcg actcattgta gtaatgctcg gcctgctcaa cagtaaccac ctggttttga
    12961 accagaatcc tcagcaaacc tacgctcata cgaccttatc cccaaattta ttcattgtta
    13021 tacctgtaca gcttttatag tggattaaat ttaaaccagt acggcgttgc ctcgccttag
    13081 ctcaaagaga acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactatct
    13141 gtactgtctg cggcttcgtc gccttgtcct gatttaaatt taatccacta tatcataaaa
    13201 caacagattt ccaagccgga acatctttta cggagcctga aaatcatttc atgattctac
    13261 cgtcctaaag gtcggttttt caagcaggaa gaaaaagttt tcagatggca aaaaagccct
    13321 ccaacactga aaggccttat atcggaaact tcccgcaaca cgggaaacag acaaatgaaa
    13381 tcgtcaaacc tcgccaacag gaatcgaacc tgtattttac gcttaggagg catacgttct
    13441 atccgttgaa ctatggcgag ccgaaatgaa aaggagattt taaccctttc cgacgacaaa
    13501 agacaagatg ccggccgcaa tcagcggccc gacagggata ccgcccataa atgccacccc
    13561 gataaccgtc ccgattaaca gccctgtaat taaaacaggc tgctgtccca tcaaaggcac
    13621 gccgcgtccc gccagccaag ccacgaaaat accgataaaa acggcggata tcattttaaa
    13681 attcaaaaat tcggcaacgg gaggaacctg cgcctttcct gaaaccaacg gactcaaaac
    13741 ccctatggtc aaaagaatga taccgagatt caacccgtgc ttctcgacca acgggacaaa
    13801 ctgtaccaat gccgtctgct gcatcagcag caatatggtt gccgagatgg taatcgaatt
    13861 gttgttgctg accaccccca acagaatcag cgtaaccaaa aacaggggaa caaaactgaa
    13921 attcatcggt tacgacaccc tgaccaaaga ttccttcgcc agctgaatca tggcatcctt
    13981 cacttcgctg tcgggcaggg catccaaggc ggcaacggca caatcgactg ctttgcgcgc
    14041 ctcgcctatt gaatatgcca acgcatccga acggacgaca taatcgtgga ttttctcaaa
    14101 atagctgcga tctgcatttt ccaaagcagt acgcacatcg ttcgcaacct gttcggaacc
    14161 ctgacgcatc agataaatca aaggcaaagt cggttttcct tccgccaaat cgtcgccgac
    14221 gtttttgccg gtttcttcgg tttcgccaga atagtccagc acatcgtcaa taatttggaa
    14281 tgccgtaccg acatacatac cgtagtcttt caacgcccgt tcgtgttcgg gggaagcctt
    14341 gcccaaaatt gcgccgactt gagcggcagc ttcaaacaat tttgccgttt tatattggat
    14401 gacttggata tattgttctt cggtaatgtc cgtattgccg atgttcatca gctgcatgac
    14461 ttcgccctcg gcaataatgt tggttgcatc cgccatcact tccaaaacgc gcatactgcc
    14521 cgaggcaacc atcagttgaa aggcgcgcgt gtataaaaag tcgccaacca acacagccgc
    14581 cgcattgccg aacagattgt ttgccgttgc ccgcccacgg cgcaaatcgc tttcatcgac
    14641 gacatcgtcg tgcaggaggg tggaagtgtg gataaactcg accatcgccg ccagcgaata
    14701 cagtttctcg tcatcataac cgaccgcctt acccgccaaa atcgtcataa tcggacgcag
    14761 gcgtttgccg cccgcgctga tgatatatgt accgatttgc gaaatcagtg cgacatcgga
    14821 ttgcaccgca cggttgatga cttcattgac tttggcaagg tcttcaggca gatgtcgctg
    14881 gaaatagggc agattctcga gcatggcaga ctctcaggtt tacgtttccg tcaaacggca
    14941 gataccgctt gaaagctgat tcaaatagcg ggcaattata tcagcaaagc catcagggta
    15001 catcttttca gacggcatat gattgtccga accgcgcaaa ctttgacaaa acaggcaaat
    15061 aacaatacaa tgacgagttc cgcacaatgg tgtgcggata ttaaccataa cccttatgga
    15121 gttgagtatg tacgcggtcg taaaaaccgg cggcaaacag tataaagttt ccgtcggcga
    15181 aaaattgaaa gtagaacaga taccagccga actcgacagc caaatcgaac tgaccgaagt
    15241 tttgatgatt gctgacggcg aatctgtaaa agttggcgca ccctttatcg aaggtgcaaa
    15301 agtaacggct aaagtagtgg cacacggtcg tggcgaaaaa gtccgcatct tcaaaatgcg
    15361 ccgccgcaaa cactaccaaa aacgccaagg ccaccgccaa aatttcaccc aaatcgaaat
    15421 cgtggcaatc gcctaacctt aaaacttagg agcattacaa atggcaagta aaaaagcagg
    15481 cggcagcacc cgcaacggtc gcgattcaga agccaaacgc ttgggcgtta aagcctacgg
    15541 caacgagctg attcccgcag gttccatcat cgtacgccaa cgcggtacca aattccacgc
    15601 aggcgacaac gtaggtatgg gcaaagacca cactttgttc gccaaagttg acggttatgt
    15661 cgaattcaaa accaaaggcg cgctgaaccg taaaactgtc agcatccgtc cttacaccgg
    15721 ttctgaagaa taatccaacc gatacctgaa gccgcatctt ttcacgatgc gg
//
LOCUS       DAWHRA010000036        15077 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWHRA010000036 DAWHRA010000000
VERSION     DAWHRA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.036"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(247..2454)
                     /gene="uvrD"
                     /locus_tag="WIB30_000849"
     CDS             complement(247..2454)
                     /gene="uvrD"
                     /locus_tag="WIB30_000849"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ3796941.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     NAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTIENLQTASNEQGITLWQAACNAGAK
                     ATKVAAFVRLIEALRNQVGQMPLPEIIVGILKDSGLTEHYKTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGADEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            2638..3285
                     /gene="pxpB"
                     /locus_tag="WIB30_000850"
     CDS             2638..3285
                     /gene="pxpB"
                     /locus_tag="WIB30_000850"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ3796942.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            3282..4211
                     /locus_tag="WIB30_000851"
     CDS             3282..4211
                     /locus_tag="WIB30_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ3796943.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQVYLNQIRRITHEAG"
     gene            4198..4935
                     /gene="pxpA"
                     /locus_tag="WIB30_000852"
     CDS             4198..4935
                     /gene="pxpA"
                     /locus_tag="WIB30_000852"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ3796944.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            5064..6257
                     /locus_tag="WIB30_000853"
     CDS             5064..6257
                     /locus_tag="WIB30_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ3796945.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            6365..7057
                     /locus_tag="WIB30_000854"
     CDS             6365..7057
                     /locus_tag="WIB30_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ3796946.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(7357..10047)
                     /gene="glnE"
                     /locus_tag="WIB30_000855"
     CDS             complement(7357..10047)
                     /gene="glnE"
                     /locus_tag="WIB30_000855"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ3796947.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRDTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTRETLKKLAELGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQRQRLAESMGFDDYADFLNAL
                     NQHRNQVNRLFNEILSEPGEQTQSNGEWQWVWQEKPDEEGRQCRLKEHGFDAEAVAAR
                     LDQIRHGHKYRHLSAHAQPRFDALVPLSVRAAAAQNNPTDTLMRLFDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWDKLAAELS
                     DGIKACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPDAGDIYGRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGSPEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(10245..10448)
                     /locus_tag="WIB30_000856"
     CDS             complement(10245..10448)
                     /locus_tag="WIB30_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796948.1"
                     /translation="MGFWNGVAKAVGEGMIEAANEHKALKMEYAEKSSEELHEIVKSD
                     GFFKNSTREKGAAYAVLKERGEV"
     gene            complement(10585..11058)
                     /locus_tag="WIB30_000857"
     CDS             complement(10585..11058)
                     /locus_tag="WIB30_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ3796949.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(11111..11392)
                     /locus_tag="WIB30_000858"
     CDS             complement(11111..11392)
                     /locus_tag="WIB30_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796950.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(11479..11802)
                     /locus_tag="WIB30_000859"
     CDS             complement(11479..11802)
                     /locus_tag="WIB30_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796951.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(12135..13142)
                     /locus_tag="WIB30_000860"
     CDS             complement(12135..13142)
                     /locus_tag="WIB30_000860"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ3796952.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGRPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGGDSADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            13374..13610
                     /gene="acpP"
                     /locus_tag="WIB30_000861"
     CDS             13374..13610
                     /gene="acpP"
                     /locus_tag="WIB30_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ3796953.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            13766..15013
                     /gene="fabF"
                     /locus_tag="WIB30_000862"
     CDS             13766..15013
                     /gene="fabF"
                     /locus_tag="WIB30_000862"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ3796954.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         3320 a         3992 c         4159 g         3606 t
ORIGIN      
        1 gaaacctttt atccgataag ttcccgtgcc gaaaggtctg gattcccgcc tgcgcgggaa
       61 tgacggcgga ggtttcggca aaatttgttg ttaccggatt cagacggcct tttgccttgt
      121 ccttgctaca gttttttgcc ataggtctga ttctcgaatc cgacattttc caacagcggt
      181 ttttcggaaa cgatagacgc gtcaaatatt tttgtcggat acgagtatcc agcctgcatt
      241 tcaaatttac atcgcttcca atttcgcaaa cttggtatcc aactctttca cgccctgttt
      301 gccgaagttg atggtcagtc gggcggattc gcctttatct gcggcatcga taatcacgcc
      361 ggtgccgaat ttggcgtggc ggacgttttg tccgatacgg aaacctgcgt aggtttgggg
      421 ctgtttgtag tcgtcgatga ttttgtcttt ggatgcggcg gtttggcgcg ggctgccgta
      481 gctgtcgtag gcggtctttt tgacggacag gtagtgcaat acttcgggtg ggatctcttc
      541 gacgaagcgg gagacgatgc cgaattgggt ttgtccgtgc agcatgcgtt gttgcgccat
      601 ggtgatgtag aggcgtttgc gggcgcgggt gatggcgacg tacatgaggc ggcgttcttc
      661 ttcgaggccg ccgcgttcgg cgagactcat ttcgctgggg aagcggcctt cttccatgcc
      721 ggtgaggaag acggcgttaa attccaagcc tttggcggcg tggacggtca tgagttggac
      781 ggctttttcg tccgcgcctg cttggttttc gccggattcg agggcggcgt tgcttaggaa
      841 ggcgaggatg ggaaaggcgg ggtcgtctga aatgttttca ggcaggattt caaagttgct
      901 gtcttcgggt ttgaactcga tggcggcgtt gacgagttcg tcgaggttgt cgaggcggtc
      961 ttggttgtca cctttttggg ttttgtagtg ttcggtcagg ccgctgtctt tgaggatgcc
     1021 gacgatgatt tcgggcaggg gcatttgtcc gacttggttg cgcagggctt cgatgaggcg
     1081 gacgaaggcg gcgactttgg tggcttttgc gccggcgttg caggctgcct gccaaagggt
     1141 gatgccttgt tcgtttgagg ccgtctgaag gttttcgatg gtgcgtgcgc cgatgccgcg
     1201 cggcgggaag ttgatgacgc gcaagagggc gttgtcgtcg tcgggattaa cggcgaggcg
     1261 aaggtaggcg agcgcgtgtt tgatttcttg gcgttcataa aagcgcaggc cgccgtagat
     1321 tttgtaaggg atgccgctgc ggaacaggct ttgttcgata acgcgggatt gggcgttgct
     1381 gcgatagagg acggcgattt cgtccaaatc ccagccttcg cgttcgaggg ctttggtttc
     1441 gtccacgatg aaccgggctt cttcgaggtc ggtaaaggcg gagtagtagc gaattttgtc
     1501 gcctgcttcg gcgtcggtgc gcaggttttt gccgagtcgt tcgtcgttgt tttcgataac
     1561 ggcattggcg gcggcaagga tgttgccgac agagcggtag ttttgttcga gtttgacggg
     1621 cgcgttgata tggaactctt ccatcagcgc ggtcatattg ccgacgtttg cgccacggaa
     1681 gcggtagatg ctttggtcgt cgtcgccgac ggcaaacact gccgcgttgc cgcccgccat
     1741 gagtttgagc caggcgtatt gcagtttgtt ggtgtcttgg aactcgtcga cgagaatgtg
     1801 gttgaagcgg ttttggtaat gctggcgcag gatttcgttg ctttgcagca tttcgtagct
     1861 gcggagcatg agttcggcaa aatcgaccac gccttcgcgt tggcagattt tgtcgtattc
     1921 ggcgtagcat tcaatcatgc ggcttgtgtg cggatcgggc gcactcaaga cggaagcgcg
     1981 caaaccggat tctttttgcg cgttgataaa gccttgcagc gaacgcggcg cgatgatttc
     2041 ttcggcgatg ttgaggcttt tgagcaggcg tttgatgagg gaaagctggt cgccgccgtc
     2101 gaggatttga aaggaagacg gcaaaccggc atcgcggtgg tgcagccgta gaaagcggtg
     2161 gcagaggccg tggaacgtgc cgagccacat agcgcggacg tcgacgggaa tcatcgcgcc
     2221 gagtcgggtt tgcatttctt tggcggcttt gttggtaaac gttaccgcca taatgctgtg
     2281 cacgcttgcc tgaccggttt gcaaaagcca tgcgatacgc gtggtcagca cgcgcgtttt
     2341 gccgctgccc gcgcctgcca gcacaagggc ggattgcggc ggccaggtta cggcggaaag
     2401 ttgttcggga ttcaagcctt gcagcaggtt gggggcggat tggtcgggaa acatagagat
     2461 gccgtctgaa aagtggaatg cgctatttta atagaaacgg tttaaggtcg tctgaaaaag
     2521 tgcagcggca gggcagcact tttccaatcg acggtttgat gatgcagcac agaagatatt
     2581 gacaaaccgc cgcccctgca tatagattca ttaaaacata accaaaatga acaacaaatg
     2641 agaatagaga tcacaccaat cagcgaatcc gctttggtct gccgactgaa tgcaccttcc
     2701 gaactgggca aacagcaaaa gttgtgggcg tttgccgctg cgctcgggca gcacgacagg
     2761 attgaggaag tggtggtcgg catgaacaat ctgaccgtgt tcacccgttt cgataccgat
     2821 ttggcgacgc ttgccgatga attgcaatat gtgtgggaac acaccgccgt tacagaccat
     2881 cagggcaaac tggtggaaat tcccgtctgc tacggcggcg aatacggccc ggatttggcg
     2941 gaagtcgctg ctttccatca gacggttatt tccgaaatcg tccgccgcca tacggcgcaa
     3001 acttataccg tatttatgat gggcttccag cccggtttcc cttatctggg cggcttgccc
     3061 gaagcattgc acacgccccg ccgtgccgtg ccgagaacgt ccgttcctgc cggttcggtc
     3121 ggtatcggcg gcagtcagac cggtgtgtat ccgttcgctt cgcccggcgg ctggcagatt
     3181 atcggcagaa ccgaattacc cttgttccga gccgatttga atccgccgac cctgctggcg
     3241 gcgggtgacc aagtccgctt tgttgcagaa aggattgagc catgattcac gtttcggcag
     3301 tgcaggcacc ggcgcatatt caggataccg gacgctacgg acaccggcgt tacggcatcg
     3361 gtcatgccgg tgcgatggac acggttgctt tggcggcggg taatatttta ttgggcaacg
     3421 acgaaggcac ggccgcaatc gaaatcgctt tgggcgggat aatgctggtg tttgaacggg
     3481 atacgccgtt ttgtctcacc ggtgccgtgt atcaggcgga attggacggc gaaccggtct
     3541 attcgtattg gcgttatacc gcccgcaaag ggcagacctt gaaaatggtg cgtgccgtgc
     3601 agggcatgta cggctatgtg tgcgtggcgg gcggatttga tgtgccggaa gtgatgggtt
     3661 cgagaagcac cgacctgaaa gccggtttcg gcggccatca gggcagaatg ctgcaaaaag
     3721 gtgattgtct ccccatcggc aaaggtgcgc aggaattgtc caaagtcggc attgccccga
     3781 taccgtttac cgataccgtc caccttgttc cttcgtcgga atatgccgct ttcagtgaaa
     3841 aagggcgtct gaatctggaa cgggaaacgt ggacgctgca aagcgatagc aaccgcatgg
     3901 gctaccgctt cgacggacag ccgctgaccc tgtcgcaacc tttggaaatg ctgtcccacg
     3961 ctgttcaggc aggaaccgtg caggtgccgc ccggcggcaa accgattatc ctgctggccg
     4021 atgcgcaaac caccggcggt tatccgaaaa tcgctaccgt tgccgccgcc gatttgggca
     4081 ggctggcaca ggtgcgcttc ggcagcaaag tcaaattcaa aataatcggc ttgaaagaag
     4141 ccaccgccct gcggcgcaaa aaccaagtct atctgaacca aatacggaga atcacccatg
     4201 aagcaggttg atttaaacgc cgatctcgcc gaaggctgcg gcagcgacga agccttgttg
     4261 cagctgatta cttcggccaa catcgcctgc gcccaacacg ccggcagcat tgccgatatt
     4321 cgggcggcat tggcgtatgc ccaacaaaac ggcgtgcgca tcggagcaca ccccggctat
     4381 cccgatcggg aaaacttcgg ccgtaccgaa atgaatctgt ccgaagccga tttgcgggcg
     4441 tgtctgaatt accagttggg cgcattgcag gccttgtgcc gtgatcaggg tttggaaatg
     4501 gcttatgtca aaccgcacgg cgcaatgtac aaccaagcgg cgaaaaaccg tgcgctggcg
     4561 gataccgttg cccgaattgt ggcggatttc gacccgaaat tgaaattgat ggcactttcc
     4621 ggcagcctgc tcttggaagc cggaaaagcc gcaggcttgg gtgtgatttc cgaagtattc
     4681 gccgaccgcc gctatatgcc cgacggtacg ctggttcccc gcagccgccc cgatgcgcag
     4741 gtggacagcg acgaagaagc catcgcccaa gtattgcaga tggtgcggga cgggcgggtc
     4801 aaagcagtga acggcagcct ggttgccgtg caagccgaca gcatctgtct gcacggagac
     4861 gggccgcacg ccgtggtgtt tgccgaaaaa atccggcagg aactactggc ggcaggcatc
     4921 aaagtttctg cttgaaaaac agttcatagg ccgtctgaaa accgcaggtt tcgccaaaac
     4981 gagcaaagcg agtttctgcg tagctaaaac cgcaggtttc gcccaaacac agcaacaacc
     5041 aattttatag aggataagcg attatgtcta atcaaaaaaa ccgcagaaat gccttaatcg
     5101 gcgctgcatt cctgatggcg acttccgcca tcggcccggg ctttctgacc caaaccgcca
     5161 ccttcaccca agcactggcg gcaagtttcg gctttgtgat tctgctctcg attctgctcg
     5221 acatcggggc gcagctcaat atttggcgga ttgtcgccgt ttccgaaaaa caggcgcagg
     5281 atattgccaa tcaggtcttg cccggcgcag gctatttctt ggctgtgctg attgtgatgg
     5341 gcggtttggc gttcaatatt ggcaacgtcg gcggcgcagg cttgggtctg aacctgctga
     5401 ccggactgtc accggaaacc ggtgccgtga tcagcggcgt gattgccatc ggtgtgtttc
     5461 tgtttaaaga agcaggcaaa gtgatggaca aattcgccca agtgatgggt ttcgtaatga
     5521 ttgcgctgac ggtttatgtg gcatggcagg cgaatccgcc gctggcagat gccgccgtgc
     5581 atacctttat gccggaaaaa ctcgatgcaa tggcgattgt tacactggtg ggcggcacgg
     5641 tcggcggcta catcaccttc gccggtgcgc accgtctgct ggacgcaggc atcaaaggca
     5701 aatcggcgtt gccggaagtg agccaaagct cggtgcgggc gatcctgatt gcctcgatta
     5761 tgcggattgt attgtttttg gcggttttgg gcgtggtcag ccaaggcgta cagctcaatc
     5821 ccgacaaccc tgcttccaca ccgtttgaat atgcggcggg atacatcggc ctgctgattt
     5881 tcggcgtggt gatttgggcg gcttcgatta cttcggtgat tggtgcggct tatacttcgg
     5941 tgtcgttctt ctccggtctc agcccgtcta tcgaacgcaa taaaaacaaa tggattattg
     6001 cctttatcgc cgtgtccacc gccgtatttt ccaccatcgg caaaccggcg caggtgctgg
     6061 tgttcgtagg cgcattaaac ggcctgattt taccgatttc cctcggtctg attctgattg
     6121 ccgcctacaa aaccaaaatt gtcggcgact acaaacaccc gctgtggctc accgtttccg
     6181 gcgtgattgt ggtcggtttg atggcagtac tcagcgccat caccatcagc aaatatatcg
     6241 gcggcttgtt cggctgaacc ttgtagcgca gatgtttgat tttgtatatt atccgaagtt
     6301 tgccgcctga tatggaaaat tcctgtttca gacgatgtat ccggtctgaa acccattcac
     6361 aactatgaaa cgcttcacct atactctttc cgacggtttg tgcatcgaaa tcgaactcaa
     6421 acgcagtgcc aagaaaaatc tgattctgcg ccccgtcaat atgcagacgg tcagcatcaa
     6481 cgtcccaccc ttttttcaag accacgcgtt agcaaactgg ctggcggcaa acgaaacgat
     6541 tttgcggaac acgcttgcca aaacgcccgt gcatcctgtt tcccacccaa acttacccga
     6601 gtggatttgg tatcggggaa taaagaccaa gctggatacc cacagccaaa gccatatccg
     6661 tatcacgtcg tctgaaatcc tgcttccccg aaaagaaacc gccgcacaaa tcgaccacct
     6721 gcgccgcctg ttgaacgaac gcgcccgcga atacctgctg ccccgccttg aaaaacacgc
     6781 agccgaaaca ggactgacgc ccaccgccac agacctgagc aacgccaaaa ccttttgggg
     6841 cgtatgccgc ccgcacaccg gcatccgcct caactggcgg ctgatcggca cgcccgaata
     6901 cgtcgccgac tatgtctgca tccacgaact ctgccacctc cgccaccccg accacagtcc
     6961 gcgcttttgg catttggtga acacgctgac gccgcatacc gacaatgcta aaagttggct
     7021 gaaggcgcac gggcgggaat tgtttgtgct ggggtgaagg ctaaccgtag cgtgggcttc
     7081 gcccgcgaga atccaccccc tcccgtacgg agggagaggg ttggagagag ggtggctctt
     7141 gtcccttgtt ctgtatattt gcaagccacc ctatcctagc cttcccccgt tggataaggg
     7201 aaagggatca agctgctgta actgaaagag gaatccggtt tttgggtttc agccattccc
     7261 gataaatcgc tttagctctg ccgcaacctt aaaacggcac ggtacgaaaa taccgtctga
     7321 aacccagatt gtcaggcttc agacggcatt ttttgttcag gcggttgccg cttcttcgcc
     7381 gaacacttcc cgccacaatt tcctgacatt gccgtaatgt gccaacagtt cgccggttac
     7441 ttcggttttt gccgcgtcgc gcagtttggt gttgtgctgc tgccggcggt agaagcggta
     7501 ggcggtgcgg ctttgtccgg caagcgtttt atcaatcaaa ccgcagtcgg cggcgatgtt
     7561 caagagggcg atgttgccgt agttgtccaa gagttgcgga tattttcggg cgtgggcaag
     7621 tatcagatat tggacgataa attcgacatc gaccacgccg ccgcgcgcgt atttgacgtt
     7681 gctgtcggca ggcgggtggg tggggaacat tttttcgcgc atttcgatga tttcgcctgc
     7741 caaggcggtt tggtcgcgtt cggcggtgag gatttcggtg cggatgcggt cgaatgccgt
     7801 ctgaatttcg ggcgagccgc agataaagcg ggcgcgggtg agggactggt gttcccacgt
     7861 ccacgcgttt tcgcgctggt atttttcaaa ggcggcaatg ctgtgggcga ggaagccggc
     7921 gtcgccgttg ggacgcaggc gcaggtcggt ttcgtagagg ctgcccgcgc cggtggcggc
     7981 ggaaagccaa ttggtcaggc ggcgggcgag ccgtccgtaa atatctcccg cgtcgggatg
     8041 ggggtcgtcg tagagataaa ccaaatccaa atcggaagcg tagccgagtt ctttaccgcc
     8101 cagtttgccg tagccgatga cggcgaattg cggggtgtcg cggtgttttt tgggcatatc
     8161 cgcccacgcg cacgaaaggg cggcggcgag gatggtgtcg gcgagggcgg agagctggtc
     8221 ggagagggat tctaccgtcc acagtccggc gaggtcttgg acggcgaggc ggaagacttg
     8281 ggcgtgctgg aaatggcgca gggtgtccat ttgcgcttcg gtgtcgccgc cgcaggcttt
     8341 gatgccgtct gaaagttcgg cggcgagttt gtcccagtca aacgccgtat ctaataattg
     8401 cgcgctgatg agttcgtcca acaaaatcgg atatttgttc agatacgccg ccacccaaga
     8461 actttggccc ataatctctg ccagttgcgc cagggtttgc ggatgttcgt tgaggaaggc
     8521 gagataggcg gagcggcggc tgatgttttc gagaaagtca aacagccgca tcagcgtatc
     8581 ggtcgggttg ttttgcgctg ccgccgcccg taccgacaac ggcacaagcg catcgaaacg
     8641 cggctgggcg tgtgcggaaa gatggcggta tttatggccg tggcggattt ggtcgagcct
     8701 tgcggcgacg gcttcggcat cgaacccgtg ttctttcaga cggcattgcc gcccttcttc
     8761 gtcgggtttt tcctgccaaa cccactgcca ttcgccgttg ctttgcgttt gttctccggg
     8821 ttcgctcaaa atttcgttaa acaaccggtt gacttggttt cgatgttgat ttaaagcgtt
     8881 caaaaaatcg gcgtaatcat caaatcccat actttcggca agccgttgcc gctgttcggg
     8941 cgaagccggc agggtttggg tttgccggtc gtcccaatat tgcaaacgat gttcgacatc
     9001 gcgcaaaaag cggtaggcgg cgagcagggt ttcgacgttt tcagacggca tgatgcccag
     9061 ctcggcaagc ttcttcagcg tttcccgcgt gcctttcagt tgcagcgcgc gcatttgccc
     9121 gccgcgtatc atctggaaaa tctgggcgat aaattcgact tcgcggatgc cgcccgcgcc
     9181 gagtttgatg ttgtccgcca tccctttttt gctgacttcg ctgcggattt ggcggtgcag
     9241 cttacgcatc gcctcatacg cgccgtaatc cagatatttg cggaacacga aggggcgcac
     9301 cagcgatttg attccgttcg gatacggcgt gaccacgcgg cctttgcacc acgcgtagcg
     9361 ttcccattcg cgcccctgcg taatcaggta ttgctccagc gcggtttcgc tcaacaccag
     9421 cgcgcccgaa tcgccgtccg gccgcagccg catatcgacg cggaacacct gcccgtcggc
     9481 ggtgatgtcg ttcaacaatg caatcagttt ctgcccgact ttggtgaaaa actcctgatt
     9541 gccccgttcg cgcctgccgt cggtatcgcc cgattcggga tagacgaaaa tcaaatcaat
     9601 gtcggaagac acgttcaact catagccgcc cgccttgccc atcgccacca cgctcaaatg
     9661 ctgcggcgat ttggtatcac gcccgatcgg cgtaccgtac atatcccgat aataggcgta
     9721 ggcgaaatcc agcgcggtat tgacggcaaa atcggcaaac agcgtaatcg tgcgggttac
     9781 ttcgttcaaa tcgctgatgc ggtttatatc gcgcacgata atctgcgaca ccacataacg
     9841 gcgcaacccg cgcaactgcc gcgccaattc ttcctcgttt tcttccgcac ggattttgtc
     9901 ccagtcggca aaggcttgga aatccgcttc ggtcaaaacc ttgtccagca taggcaggaa
     9961 aatttcgggc ttgagtttgc cgttgtcgag ctggcgggcg aggaagaggg aatggcggcg
    10021 ggcggtgtcg aggcggttgt cggacatttc ggattccgtt tgaaaaaatg gcaggaagat
    10081 gatggcggat tcacttcaaa tcaggacagg gcgaggggat gccgtaccgg tttaaagtca
    10141 agccactata tcatagcaac cttcgatgcc gtctgaaacc ttgccccgac atccagacgg
    10201 atgcctgccg ataaaaaaca gcggcaaatg ccgccgtttc ctgttcacac ctcgccacgc
    10261 tcttttaaaa cagcataagc cgcacctttc tcccgtgtgg aatttttaaa aaaaccatca
    10321 ctctttacga tttcatgcag ctcctcactt gatttctccg catattccat tttcaacgcc
    10381 ttatgctcat tggctgcttc aatcattccc tcgcctactg cttttgccac accattccaa
    10441 aaacccataa tcatgtcctc caatcgtttc aaataaataa ggtcgttgtt tctgtcaaga
    10501 aacaacgaca gtttataaac tgtcggaaaa caaaatgccg tctgaaggcc ggataatgct
    10561 tcagacggca tcggattgcc ggttttaacc ctgccctccg ccgttgttca aatagtgttc
    10621 ccagtgagaa tataaccggg caatattttc ttcataggtg tgggcagaag gaatataacc
    10681 gtctttgggc ggcatatacc tgacaaatga ttctatttct tccggataat caaaatctgc
    10741 ataaattttt tcaacttccc ccaaaggatc ctcaaattct tcttttctat ggaatacatc
    10801 gcttaagagg acgtacagcc aatttttagg ggaataatcc ctcatttccg aagccaaatc
    10861 ttccagaaca ggctttaatt caagaatatt ggatttgttg attaaagata gtttgaacac
    10921 acgttcatca tgatctgaaa gcgtcatttt tcgggcataa gctgccacat cagcccaacc
    10981 caagtattta ttcccatacc cccacagaat atccttccaa gaaagacgga ctttttgcgc
    11041 ggttaaatca aggttcattt tttactccga ttgcctgccc cgtcattccg ccccttccga
    11101 ttgacgcctg ttacgaaaag tcttccaaat gacggctgat tgcctcttca acagtcggat
    11161 gccaatccat aaatgaagcc tgcaattcct ctaaaaccaa ctcattgaat aaatcatcca
    11221 aattccgcca atccgttttt tcatccggat agatgaaatt tgccataacg acagtatgtt
    11281 tgttttggga ataatccgat aaggcgcaaa tcatcagata ccgccccgaa tgctccagat
    11341 aaaaaattcg tttttccaaa ggaaaccggt tattccgttg cccgttttcc atttttcatc
    11401 ctttttaacg tatcggcttc cgcgtccttc aaattgagtt acctgccttc cgccaagcaa
    11461 acgcccgcgc ccctttcatc atttgaatat ccgtttgacc atatttacca gataactatc
    11521 ttctttatat aaatcaatca gcaaaggcac gatacccggc tcataaaata attctgtcgt
    11581 atcagatgtt tcttttaacg cggaaattat ctccaacagt tcatacccgt atcggggata
    11641 agcaaacaaa taggggactg cttcccgcaa acatgaaaat gcttcgtaac tttgcatttc
    11701 ccatatatgt tcatctttaa aatcactcaa ataccattca tccaattcac tttccggatc
    11761 ggatatcaat cggataaatt catttagttc gtccttaaac atatcaaatc cttaaatata
    11821 aaatcttttg attatccgcc gtatccttat tttccacatc atgaatttca acgccgttca
    11881 tattttgcgt tttcccaagc cgtccgccac agtataaaat tattttttgt atcaattgct
    11941 tatatattta acaacttacc gccggacttg ctttggctgc gtatcaagat tgtccggttc
    12001 gcggcgggcg tttgctttgt ttcaaacagg gcggcgcgta agggattttc aaggggcgtc
    12061 aggcggaaaa aggaaatgaa aatgccgtct gaacggcaaa acgtgcgttc agacggcatt
    12121 ttgggcggat cgcgtcatcg cgccaaagcc ttcaaacatt ctttgaccaa tgccggacct
    12181 ttgtatatca atccgctgta cacctggacg gcagttgcgc ccaagcggat tttatctgcc
    12241 gagtccccgc cttccataat gccgcctacg ccgataatcg gcagcttgcc gtctatgtgg
    12301 tctgccaaca gcttcaacac ccgattactt ttttcatgaa cgggccgccc gctcaaaccg
    12361 ccctgctcgc ctgcgagcgg atggctgccg agacttgatt tgtcgatggt ggtattggta
    12421 gcgatgatgc cgtccatttc gacggatttg acaacgtggg cgatgtcttc gatttgtgct
    12481 tcatccaaat cgggggcgat tttgacggcg agcggaacgt atttcccgtg tacagaggca
    12541 agctgtgcct gtttgttttt caaagcctca agcaatgcgc tcaactcgtc gccaccttgc
    12601 agcgcgcgga ggtttttggt gttgggcgag gaaatgttga cggtaatgta gcttgcgtgc
    12661 gcgtaggctt tttcaaggca gattaaataa tcatcggcag cgttttcgat gggtgtaacc
    12721 gcgtttttac cgatgttgat gcccaatacg ccactgaatt tacttttttc gatgttttga
    12781 atcatggcat cgataccgtg gttgttgaaa cccatgcggt tgatgatgcc ttggtgttcg
    12841 ggaacgcgga agaggcgcgg ctgcgggttg ccgggctgcg gtttgggcgt taccgtgccg
    12901 atttcgatga aaccaaagcc gagcgcgccc aatgcgtcga tgtattcgcc gtttttgtcg
    12961 agtccagcgg caagtccgac agggttgggc aaatccatac ccatcaattt tacaggtttg
    13021 gtacggttgc cggttacggg aatcagaccc aatttataaa ccgtgtagag cgcgtcgagc
    13081 gtgaagtggt gggctttttc ggcatcgagt gcaaacagga tgcggcgggc aagtggatac
    13141 atgacagggc tttctgcttc agatggaaat gccgccattt taaccgaaat cccgccaaac
    13201 atacggcaca caggtcaaac ttgaacgaca aaacccgccg cacccaaaaa tgtacaaatc
    13261 ggcaaatatt gatataatag tcattagata gaaaactcta ttaaaagtcg gtacaatacc
    13321 accgatttat ttcccttaaa caacctgccg caaggcataa aggaacgact gatatgtcaa
    13381 acatcgaaca acaagttaaa aaaattgttg ctgaacaact gggcgtaaac gaagccgacg
    13441 tgaaaaacga atcttctttc caagacgact tgggcgcgga ttctttggac accgtggagc
    13501 tggttatggc tttggaagaa gcattcggct gcgaaatccc cgacgaagat gccgaaaaaa
    13561 tcaccaccgt ccaactggct atcgactaca tcaatgccca caacggctaa ccggtcgtcg
    13621 cccgacacaa cagcctctgc tgcaccgcgc aatagaggct tttcccttat atggcaaact
    13681 gtttgaaccc ctgccgcaaa ttacagacgg catctgcacg acagccgcat atgcccccat
    13741 tccgacaacc aacagcgagg ttatcatgag tcagagaaga gtagtcatta caggcttagg
    13801 tcaggtttcc cctgtcggca acactgtcgc agaggcttgg gacaccctgc tcgccggcaa
    13861 aagcggcatc ggcgcgatta cccgctttga cgcatccgac atcaacagcc gtgtcgccgg
    13921 cgaagtgcgc ggtttcgaca tcggacaata catcagcgcg aaagaagcgc gccggatgga
    13981 cgtattcatc cactacggca ttgccgccgc attgcaggca atcgccgatt cgggcttgga
    14041 cgatgtggaa aacctcgaca aagaccgcat cggcgtgaac atcggttccg gcatcggcgg
    14101 actgcccagc atcgaggtca ccggcaaagc cgtaatcgaa ggcggcgcgc gcaaaatcaa
    14161 ccctttcttt atccccggtt cgctgatcaa cctgatttcc ggacacgtta ccatcctcaa
    14221 aggctaccgc ggcccgagct acgggatggt ttccgcctgt accaccggcg cgcacgccat
    14281 cggcaattcc gcccgactga tcaaatacgg cgacgcggac ataatggttg ccggcggcgc
    14341 ggaaggcgca atcagcactt tgggcgtggg cggttttgcc gcgatgaaag ccctctccac
    14401 ccgcaacgac gaccccgcca ccgcttcccg tccgtgggac aaaggccgcg acggcttcgt
    14461 tatcggcgaa ggcgcgggca tattggtgtt ggaagaattg gaacacgcca aaaaacgcgg
    14521 cgcgaaaatc tacgccgaaa tcgtcggctt cggcatgagt tccgatgctt accatatcac
    14581 cgcgccgaac gaagaaggcc ccgcccttgc cgttacccgc gcgctgaaag atgccggcat
    14641 caatcccgaa gacgtggatt acgtcaacgc gcacggcacg tccaccccct tgggcgatgc
    14701 caacgaaacc aaagccctca aacgcgcatt cggcgaacac gcctacaaaa ccgtcgtcag
    14761 ctcgaccaaa tccatgaccg gccacctgct cggcgcggcg ggcggcgtgg aggccgtgta
    14821 cagcattttg gcgatacacg acggcaaaat cccgccgacc atcaacattt ttgaacaaga
    14881 cgttgaagcc ggctgcgatt tggactactg cgccaacgaa gcgcgcgacg cggaaatcga
    14941 cgttgccatt tccaactcct tcggcttcgg cggcaccaac ggcacgctgg tcttcaaacg
    15001 cttcaaaggc tgattccgca aagccgccgc cgacatcgaa atgccgtctg aaaccgtttc
    15061 agacggcatt tttatag
//
LOCUS       DAWHRA010000037        14916 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWHRA010000037 DAWHRA010000000
VERSION     DAWHRA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14916
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.037"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(101..3586)
                     /gene="smc"
                     /locus_tag="WIB30_000863"
     CDS             complement(101..3586)
                     /gene="smc"
                     /locus_tag="WIB30_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ3796955.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHTGQTIAKHEECKGRLKQENQALNLPDEAETAAAQEAAALLQSQ
                     QEHYEEQIIAAEETLHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPQGFVPPAPLPQGQAAWL
                     SDDLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQNNLSAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELESLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLLDDISTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIAQLEQQTFDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQAQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNTSIGSLTQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3588..3839
                     /locus_tag="WIB30_000864"
     CDS             3588..3839
                     /locus_tag="WIB30_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796956.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     ALEPRFRQCPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            3836..4558
                     /gene="lpxH"
                     /locus_tag="WIB30_000865"
     CDS             3836..4558
                     /gene="lpxH"
                     /locus_tag="WIB30_000865"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ3796957.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            4827..6413
                     /locus_tag="WIB30_000866"
     CDS             4827..6413
                     /locus_tag="WIB30_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ3796958.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6581..6784)
                     /locus_tag="WIB30_000867"
     CDS             complement(6581..6784)
                     /locus_tag="WIB30_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ3796959.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6900..7397
                     /locus_tag="WIB30_000868"
     CDS             6900..7397
                     /locus_tag="WIB30_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ3796960.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7479..8672
                     /locus_tag="WIB30_000869"
     CDS             7479..8672
                     /locus_tag="WIB30_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ3796961.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9075..10010)
                     /gene="hemC"
                     /locus_tag="WIB30_000870"
     CDS             complement(9075..10010)
                     /gene="hemC"
                     /locus_tag="WIB30_000870"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ3796962.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESESLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10129..10644
                     /gene="ybeY"
                     /locus_tag="WIB30_000871"
     CDS             10129..10644
                     /gene="ybeY"
                     /locus_tag="WIB30_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ3796963.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            10646..11470
                     /locus_tag="WIB30_000872"
     CDS             10646..11470
                     /locus_tag="WIB30_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ3796964.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11599..12978)
                     /gene="nhaC"
                     /locus_tag="WIB30_000873"
     CDS             complement(11599..12978)
                     /gene="nhaC"
                     /locus_tag="WIB30_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ3796965.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13269..14492)
                     /locus_tag="WIB30_000874"
     CDS             complement(13269..14492)
                     /locus_tag="WIB30_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ3796966.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3524 a         4199 c         3831 g         3362 t
ORIGIN      
        1 caacgctgta ccgatttaaa tttaattcac tataccgtct gaaaacccgt atcgcaacac
       61 ttcattaaaa acggcaagat tcagccgttc tgcaacccgt tcaaacggct tccgccattt
      121 ctaacgcctg tttgatgtcc acggcgacga cgcgcgagac acctttttcc tgcatggtta
      181 cgccgaccag ctgctccgcc atttccatgg tcaggcggtt gtgggagatg tagaggaact
      241 gggtttgcgc cgacatttct ttgaccaggt tgcagaagcg cgaggtgttg gcgtcgtcca
      301 gcggggcatc gacttcgtcc aaaaggcaga acggagcggg gttgaggctg aacagagcga
      361 acacgaggct catggcggtg agggcttttt cgccgccgga gaggaggtgg atggtgctgt
      421 ttttcttgcc gggcgggcgc gccatgatag acacgccggc ggtcaaaagg tcgtcgccta
      481 tcattttgag ggtggcttcg ccgccgccga acagggtcgg gaagaaggtt tggactttgc
      541 cgttgacggc atcgaaggtt tctttgaaac gcgctttggt tttgtcgtcg atttgggcga
      601 tggcttcttc caaaatggtg atggcggcct gcacgtcttc gctttggctg cggtagtagc
      661 cgtcgcgttc gcgcgcttct tcgagttctt gcagggcggc gaggttgacg gcgccgaggg
      721 cttcgatttg ctgggtaagg ctgccgatgc tggtgttcaa tactttcggc gattctttcg
      781 ccaacgcttc gagcgcgtcc aaatcggcgg cgcgttcggt caagttttga tggtagcgtt
      841 tggcgttaat cagggcttcc tgctgctgca acagggcggt ttgggtagcg gcctgaagct
      901 gtggcaactg ggtttgcagg gtttgtacgc gggcgtattg ctccctgcct tgcgcctgaa
      961 tctgtgcgag tttttcttgt acgacgatgt attcttcgtc caaggtctgt acggcttcgc
     1021 ttaattcttc aagcttgatg tgctgctcgt cgttttggaa ctcggtttca taggcaaggg
     1081 caagctcttg ctggcgttcc tgccagtcga aggtttgctg ttcaagctgg gcgatttgct
     1141 gttggtagtt ttgtttttgc tggttgagct tgtggacggc gacttcggca agcccgtatt
     1201 ggcggttggc ttccaacagg gcaagctgcg cctgtttcag acggccttgc tgctcttggc
     1261 ggctgtgtgc ggtggtttgc tgttggtgtt caagttcggc ggcggcttcc tgcaaagtag
     1321 agatgtcgtc taaaaggccg tcggacgtgt gttgcaacac ggtctgttct tccgccaact
     1381 gcgccagttc gcgctcgatg tgttcgcggc ggatttgccc ttggttggta cgcgccagaa
     1441 gttcggcggc acgttgctgc gcttggctgt attggcgcgt gtgctgctgt tgttgctgca
     1501 tcaggttttt gtgctgcact tcggacgagc gcacggcggc ttccgcctgt ttgaacgcgg
     1561 cttcggcggc agaaagttcg ggggcgaggc tttccagttc ggaagcgatg ccgtcgaggc
     1621 gcgctttttg ggcaatcagg ctttcctgcg cgggtttggc atagagcagg acgctgactt
     1681 tgtcgacctg atggccttca ggcgtgagcc agatttggtg tgcgctcaaa ttgttttgat
     1741 gcgcgagggc gtagctcaaa tcgggtgcgc acaatacgcc gtcgagccag tggtgcaatg
     1801 ccgtctgaaa cggcggctgc gcttggattt ggttcagcaa tgcctgtacg ggcagggatt
     1861 ttttgatgcc gccggagagg tcgtctgaaa gccacgccgc ctgcccttgc ggcaatggcg
     1921 cgggcggaac gaaaccttgc ggcacgctac gggcgtgcag gcgttcggca aggatgacgg
     1981 acaaggcgtg ctgccactcg gcgggcgcgg tgatgtgttg ccacagttgc ggcgcggcgg
     2041 cgtggtcggt tgcctgccag aagtcggcgg cttcctgctg ttgcgacagg atttgcgaca
     2101 acgcctgctg ctgcgcctgc aaggtgatgt gttgctgctt caggctttgg aagcggtttg
     2161 aggccgtctg aaacgcctcg cgggcggcgt gtaaggtttc ttcggcggca atgatttgtt
     2221 cttcgtaatg ctcttgctgg ctttgcaaca aggcggcggc ttcctgcgcg gcggcggttt
     2281 ccgcttcgtc gggcaggttc agggcttggt tttcctgttt cagacggcct ttgcactctt
     2341 cgtgcttggc aatcgtttgt ccggtatggg caagctgctg ctgcttcaac gccagttcgc
     2401 ggcggatgcg gtttgcctcg tcctgctggg tttggaaggc ggcgttgagc gtggcttggg
     2461 cttcttccaa ttcgggcaga cgctcttcgt gttcggcaac ctgcatcgcc cactccgcca
     2521 gctcggtttg cttttcttcg acgtataact cgttttcttc aagctgcacg cggatttgct
     2581 gctgctcttg atgaatacgc tgcaactgcg cctgtgccgc ctgcttgtcg cgttcgatgc
     2641 gttgatgcag gttttgccgg tggcggattt gttcttccaa acgggcaatc tgctcgcgca
     2701 acacgccgcg tttgttgccc aattcgtgta ccgcctgctg ctgcgactgt tcggcggtct
     2761 gcaaggcatg tacttcgtcg tttaacgcct gaacctgcgc ggcggtttcg tcctgctgcg
     2821 cctgcaaaga ctgatgctgc gcggtcgctt tgtcggcggc ggcaagcgat tgctgccatt
     2881 gggcgtaatc gagcaaatcc tgttgttggt tcaactgcgc ggtcagggat ttgtagcgtt
     2941 cggcagtttc ggcttgtttt tccagctttt ccacctgacg cgccaactcg ttctgcaaat
     3001 cgcccaaacg ctgcaaatgc tcgcgcgtgt ctttcagacg accctccgtc tccttgcggc
     3061 gttccttata tttggacacg cccgccgcct cctcgatata agcgcgcaac tcctccggcc
     3121 gcgcttcgat gatgcgcgaa atcatcccct gctcgataac ggcataaccg cgcgcgccca
     3181 cgcccgtacc cagaaacaaa tcagtaatgt cgcggcggcg cacggtctga ttgttgatga
     3241 aatatgtcga ttcgccctga cgcgtcaact gccgcttgat gctcacctcg gcatactgcc
     3301 cccacgcccc ctgcaaactg tggtcgctgt tgtcaaacac cagctccacc gaagccctcg
     3361 gcgcaggacg gcgcgtcgcc gcaccgttaa aaatcacgtc ctgcatactc tcgccacgaa
     3421 gctgcttcgc cgaagcctcg cccaacaccc agcgcaccgc gtcaatcaca ttcgacttgc
     3481 cgcagccgtt cggcccgata accgcaacaa gctgccccgg aacatgaatc gtggtcgggt
     3541 cggtaaaaga tttgaagccg gagagtttga tgtgggtcag gcgcataatg gtcggaaaaa
     3601 ataaaaaaga agcgtatttt aacggaaatc ccgccgcacc accccatatc ttgccggcaa
     3661 aaccttaccg catcccgcac ccgcgatgcc gtctgaagcc ttcagacggc atttttaacg
     3721 cgctcgaacc ccggttccgc caatgcccga tgcttggcgc ggcatttgcc gtattgcccc
     3781 gccgccgggc gcgttataat ccgccacgac atccgcccga tttggaaacc tgcctatgaa
     3841 acccgcctat ttcatctccg acctgcattt gagcgaaaag caccccgaac tgaccgcgct
     3901 gctgctgcgt tttttgcgtt cttccgccgc cgggcaggcg cgggcggttt atattttggg
     3961 cgatttgttt gatttttggg tgggcgatga cgaagtttcc gagttgaata cttcggttgc
     4021 gcgtgaaatc aggaaattgt ccgacaaagg cgttgccgtg ttcttcgtca ggggcaaccg
     4081 cgacttcctg atcggtcagg atttttgccg gcaggcgggt atgacgctgc tgccggatta
     4141 ctcggttttg gacttgttcg gctgcaaaac cctcatctgc cacggcgaca ctctgtgtac
     4201 ggatgacagg gcatacctgc gtttccgccg catcgtgcat tgcaggcggc tgcaaaaact
     4261 gttcctgatg ctgcccctga aatggcgcac gcgccttgcc gccaaaatca ggcgtgtcag
     4321 caaaatggaa aaacaggtca agcccgccga tattatggat gtcaatgccg cctttaccgc
     4381 gcggcaggtt cgcgccttta acgcggaaag gctgatacac gggcacaccc accgcgagca
     4441 tatccatcac gaaaacggct ttacccgcat cgttttgggc gactggcata acgactatgc
     4501 ttcaatcctc cgcgtagacg gggacggcgc ggtattcgtg ccgctggaaa aatgctgaaa
     4561 atgccgtctc aagcctttcg ggcggctttt tttacacgcc cctttcccac actccctccg
     4621 ccgcatcatc accatcctta acccgcatta aggggcgtta tcgataaaat gcttgaccga
     4681 ttacataaga ttacgtaaag tgtgcaaacg cataacaatt ggtcttacca atttatcttt
     4741 taaccaaaat atcgggtgta cgggatttga acgtctttcc aaacctcgac gcgccttatg
     4801 atgcaaatgc ctaaaaagga ttcgttatgg cacttttcct cagcatattc cccatcgtcc
     4861 tgctgatttg gctgatggtg aaaaaaaaca gtatgccctc ctacgtcgcg ctgccgatta
     4921 ccgcagtgct gatttacgcc atcaaacttt tctacttcga cgatgcgggc atgctgctca
     4981 acgccaccgc cgcttccggc ctcgtcaaaa cgctcacgcc gattaccgtg attttcggcg
     5041 cgattatgtt caaccgtatg atggaaacca cgggctgcat cgatgtcatc cgcaaatggc
     5101 tggcgaccat cagccccaac cccgtagcgc aactgatgat tatcggctgg gcttttgcct
     5161 ttatgattga aggcgcatcc ggcttcggca cgcctgccgc gattgccgcg ccgattctga
     5221 tgagcttggg cttcaacccg ttaaaagtgg cgattttcac tttggtgatg aactccgtcc
     5281 ccgtctcttt cggcgcggtc ggtacgccga cctggttcgg tttcgcaccg ctgaacctga
     5341 gtgccgaaga catcctcgcc atcggcaggc agaccggcgt aatgcacttc ttcgcaggtt
     5401 tcgtcatccc cgtcatcggc ttgggcttca tcgtaccttg gtctgaaatc cgcaaaaact
     5461 tgggcttcgt cgccattgcc gtcttctcct gcaccattcc ttatgtcgca ttggcgatgg
     5521 tcaacgaaga attcccgtcg ctcgtcgccg gcgcaatcgg cctgatggtg tccgtattcg
     5581 ccgccaacca aggctggggc ttgagcaaag accacgccaa agacccgaat gccgaaaaag
     5641 tgccgttcgc ccaagtcgcc aaagcactcg cccctttggg tatgctgatc ggcatgctgg
     5701 tggttacgcg catcaaacag ctcggcatca aaggcatttt gaccagcaaa gaagaatggt
     5761 tcagcttcca actgccgttt gatttgtcca aaatcaccgt cagcgactcc ctgacgatta
     5821 ccttcggcaa tattttcgga caagatgtca gcgcgtctta ccaaacgctg tacgtcccgg
     5881 cttggattcc gtttgtgctg accgtttgga tttgcatcct gctgtataaa accaaattca
     5941 aagatgcctg gacgatttat tccgtaactt tcaatcaaac caaaaaaccg ctgcttgccc
     6001 tgatgggcgc gctgattatg gttcagctga tgctggtcgg cggcgacaat tcgatggtga
     6061 aaatcatcgg taaggaattt gccgcaatgg cgggcgaaca ctgggtttat ttctcgccgt
     6121 atctgggcgc gatcggtgcg ttcttctccg gctccaacac cgtgtccaac ctgaccttcg
     6181 gaccgattca gcagcaaatc gccttggata ccggcctgtc cgttaccctg attctggcgt
     6241 tgcagtccgt cggcggcgcg atgggcaata tggtgtgcct caacaacatc atcgccgtat
     6301 gtaccgtatt ggatgtgaaa aattccgaag gtgcgattat caagaaaacc gttatcccga
     6361 tggcgattta cggcgtgatt gccgtcgtcg cggcaatgat tttcttcctc taggcgaaac
     6421 gccaccatcc atagcggcag cacgcgggga acggaaaccg ccgttccccg tccgggcggc
     6481 gcgtcgggca gaatctcggt gccggaacag cccgccccgc caaacaaatg ccgtctgaaa
     6541 ccggaaaagg cttcagacgg catttttccg ccgcttgcgt tcaggcgtaa tgatgatgcg
     6601 gcatcttgcc gtcaaggcgg tagcgcgtgc cgcaatacgg gcaggcaacg ctgcccgatt
     6661 ctccttcgca caaaggcaaa aacactctcg gatgcccgtt ccactgctcg ttgtcgggtc
     6721 ccgagcaata cagcggcaga ttttccggca acacggaaat ttcctgcaga ttcagattgt
     6781 ccatttgatt ttcctttgcg tggttggcgt gcgcccattt tacgccatcg gcaggctaaa
     6841 ggatattttc ggcgcaaagc cgcaatccgc tataatcccc acttttcaga cggcatacca
     6901 tgactgcgct tacccttccg gaagacatcc gccaacaaga gccatccgcc ctgctctata
     6961 ccctcgtttc cgcctacctc gaacacaccg cccaaaccgg cgacgaatcc ctctcctgcc
     7021 tgtccgacga ccagcacacg ctgaccgcat tctgctacct cgacagccaa gtcgaagaag
     7081 gcggcttcgt gcaactcatc gcctccggtt acggcgaata tattttccgc aacccgcttg
     7141 ccgacagcct gcgccgctgg aaaatcaaag ccgtgccgaa agtcttggac aaagccaaag
     7201 ccctctacga acaacacggc aaaaccatcg aaacgctcgc cgacggaggc gcagacatcg
     7261 aatccctgcg caaacagttc cccgactttg aagaatggga cggcgcatac tacgaagccg
     7321 ccgaacaaga cctgcccctg cttgcagaac acatacagtc aaactgggaa accttcgccc
     7381 atatcgggca ggcgtgattg cgtctgtttc cagcccgtgt aaaacagcgt aaaatcggca
     7441 aacccgaatg accttccgtc cccatcaagg agcaagctat gttcttcaag cacatcgaag
     7501 ccgcccccgc cgatccgatt ctcggtttgg gcgaagcgtt caaagccgaa acccgccccg
     7561 aaaaagtcaa cctcggcatc ggcgtttata aagacgcatc cggcgcgaca cccctcgtca
     7621 aagccgtcaa agaagccgaa aaacgcctgt tggaaagcga aaccaccaaa aactacctca
     7681 ccatcgacgg cgttgccgac tacaacgcgc aaacccaaat cctgctgttc ggcaaagacc
     7741 acgaaatcat cgccagccgt cgcgccaaaa cagcgcaaag cctcggcggt acgggcgcat
     7801 tgcgtattgc ggccgagttt gccaaacgcc agttgaacgc gcaaaccatc tggatttcca
     7861 atccgacttg gcccaaccac aacgccatcg ccaaagcggt cggtatccaa gacaaacctt
     7921 atcgttacta tgatgccgcc aaacacggtt tggattggga cggcatgatt gaggacttga
     7981 gccaagcgca aaaaggcgac atcgtcctgc tgcacggctg ctgccacaat cctaccggca
     8041 tcgaccctac gcccgaacaa tgggaaactt tggcaaaact ttctgccgaa aaaggctggt
     8101 tgccgctgtt tgactttgcc taccaaggct tcggcaatgg tttggaagaa gatgcctacg
     8161 gcttgcgcgt gttcttgaaa cacaatacag aattgctgat tgccagctct tattccaaaa
     8221 acttcggtat gtacaacgag cgcgtcggcg cgttcacttt ggtggccgaa gatgaagaaa
     8281 cagcagcccg cgcccacagc caagtcaaaa ccatcatccg taccttgtat tccaacccgg
     8341 cttcacacgg tgcgaacacc attgcgctgg tgttgaaaaa tgatgatttg aaagcacaat
     8401 ggattgccga actcgatgaa atgcgcggcc gcatcaaagc catgcgccaa aaatttgtcg
     8461 ggttgctcaa agccaaaggt gcaagccaaa actttgattt cattatcaaa caaaacggta
     8521 tgttctcttt cagcggcttg actcccgaac aagtcgaccg cctgaaaaac gagtttgcca
     8581 tttatgccgt ccgctccggc cgcatcaacg tcgccggcat taccgacgac aacatcgatt
     8641 atctgtgcga aagcatcgtg aaagtcttgt aaaacaaaag cgcatacgaa atgccgtctg
     8701 aaccttaacc agttcagacg gcatctgttt tttgttaaaa aacaataaac tataacaatt
     8761 tcaaacaagc taaaaacatc tataaaatca acccaatatg atgaactcaa ataaaaaata
     8821 cgaatatcat cattctcaaa ttcaaaattt gaacgccaca gtattatccg aagccacaaa
     8881 aacctttccg ccgtcattcc cgcgcaggcg ggaatccggg aacccaatgc tgcaagaatt
     8941 gattagaaac aactgaaacc gagcaaactg gattcccgcc tgcgcgggaa tgacgaaata
     9001 aggcgagtaa aactgatgaa acaaattgat taaaaacaat aacttatatt attttattta
     9061 aattttcgat aaaatcaatt ttccgtattc aatactgctc caatcaattc ccgcgcaccg
     9121 tcgtccgcca atttcttagc gacagcgcgt ccgagcgcgt cggcatattc ggcaggggct
     9181 tgcgcgtccg cccgcaacac aaccgaaccg tcgggatgtc cgaccagtcc gcgcaaggtc
     9241 agcaagccgt tttcttctgt gcaatatgcg gccaaaggca cttggcagct tccgcccaaa
     9301 gcgcgtgcga gggcgcgttc ggcggtaacg caggcattgg taacgccgtg gttcaagggt
     9361 ttcaaaactt catacaaatc ttcgcggtgc gcggcaattt cgatacccaa tgcgccttgt
     9421 ccggcggcag gcaggctctc ggattccgac aaaatcatgc ggatgcgtcc gtccaattct
     9481 aaacgctgca aaccggcggc ggccaagata attgcatcgt attcgccgtt atcgagtttg
     9541 gacaaacggg tttgcacatt gccgcgcaaa ggtttgataa gcaaatgcgg atagcgcgca
     9601 cgcaactggg cttcgcggcg caggctggat gtgccaacaa ccgcgccttc gggcatttct
     9661 tccaaacgcg tgtattggtt ggacacaaat gcgtcaaacg gattggcgcg ttcgccgatg
     9721 gccgcaagcg cgaaaccttc aggcaaatcc atcggtacgt ctttaatcga atgcaccgcc
     9781 aaatcggcgc gcccgtcata taaagcctgt tccaactctt tgacaaacaa gcctttaccg
     9841 ccgacttttg acaaagtttt gtccaaaatc tgatcgccgc gcgtggtcat gcccaaaatc
     9901 tcgacttcgc aatcgggata cagagccttc agacggcctt ggatatgctt tgcctgccac
     9961 atagcaagca ggctttcgcg gctggcgatg acgagttttt tcgggttcat gttcaatgcg
    10021 ttcaaagaat ggaaacggcg tgaagtctat catattttga acgtttcttc agacgacacc
    10081 gtcaggtcta tgttatattt acacccacaa aacacgaaac ccgccacaat gaaacgcacc
    10141 aaaaaatacc ctttcttaac gttgcagcgg caacgtttcc atttgaactt tgaaaacgcc
    10201 tcttccgccg ccggcatccc agccgaacgc gatttctacc gctgggcgtg gtctgccttg
    10261 aaaaatgaat accgccgcgc cgacatcagc ctgattcttc tggacgaaga agaagcccga
    10321 gcctacaacc gcgactaccg cggcaaagat tacgccacca atgtattgag tttcgcgctc
    10381 aacgaaggcg aaatcctgcc ctgccaagtt tcggaaaaac tgtacggcga tttgattatc
    10441 tgcccgcaag tcgttttaaa agaagccgcc gaacagggca aaacgcccga acggcatttt
    10501 gcccacctga ccatacacgg tactttacac ctgatgggct acgaccacat cgaagacgac
    10561 gaagccgaaa taatggaagc cgaagaaatc cgcctgatgc tggcggcagg cttccccaac
    10621 ccctaccaag aggacgaata ttaaaatgga cggtacgcag tcgaaaccca agtttttcga
    10681 acgcctgatc tcccgacttg ccggcgagcc cgactccgcc gaagacgtat tgaccctgct
    10741 gcgccaagcg cacgagcagg aagtttttga tgcggatacg cttttaaggt tggaaaaagt
    10801 cctcgatttt tccgatttgg aagtgcgcga tgccatgatt acgcgcagcc gtatgaacgt
    10861 tttaaaagaa aacgacagca tcgagcgcat caccgcctac gttatcgata ccgcccattc
    10921 gcgcttcccc gtcatcggcg aagacaaaga cgaagttttg ggcattttgc acgccaaaga
    10981 cctgctcaaa tatatgttta accccgagca gttccacctc aaatccattc tccgccccgc
    11041 cgtcttcgtc cccgaaggca aatcgctgac cgccctttta aaagagttcc gcgaacagcg
    11101 caaccatatg gcgattgtca tcgacgaata cggcggcaca tccggcttgg tcacctttga
    11161 agacatcatc gagcaaatcg tcggcgaaat cgaagacgag tttgacgaag acgatagcgc
    11221 cgacaatatc catgccgttt cttccgaacg ctggcgcatc catgcagcta ccgaaatcga
    11281 agacatcaac accttcttcg gcacggaata cagcagcgaa gaagccgaca ccattggcgg
    11341 cctggtcatt caagagttgg gacatctgcc cgtgcgcggc gaaaaagtcc ttatcggcgg
    11401 tttgcagttc accgtcgcac gcgccgacaa ccgccgcctg catacgctga tggcgacccg
    11461 cgtgaagtaa gcaccgccgt ttctgcacag tttaggatga cggtacgggc gttttctgtt
    11521 tcaatccgcc ccatccgcca aacataaaaa tgccgtctga aaccgttgca agtttcagac
    11581 ggcatttcgc ttatccgctt attttttgct caaagtcagc cccgtccaac cgaataacag
    11641 ggtcaaagcc aaactcaaat agcagaaaaa ggcatacggc agatattccc aaaccggcac
    11701 gcccagcgcg tggctgatga acacgccgca tacgctccac ggtacgagcg ggttaatcac
    11761 cgtccccgca tcttccagcg tccgcgacag attgcgggaa tgtagtccga gcctgtcgta
    11821 aacgggttta aacgtttctc cagaaagcag gatgctcaaa tattgttcgc caatcaggaa
    11881 attaaccccg accgaagtca tggcaacgct gaacgtcgcg cgtccggcat tcgtcaagaa
    11941 ggtacggatg gcctccaaca gggaaggaat cacaccgagc gcaaacaaca gtccgcccaa
    12001 actcatcccg agaatcacga tggtttgcgt gaaaaacata ctttccaaac cgccgcgcga
    12061 aatcagtttg acaacatctt taaacgcttc gccttcgagt ttgtagccgc cgtaaaacca
    12121 cgcaccgagc tgacgcagat cgggcgtgct gtgcagatac gttacagcaa cggcaaccat
    12181 gacggtaaag agcatggcga cgacggcgtt gatgcgcatc aatgccaaaa tgaccaacag
    12241 cgcaaacgga atcagcgaat agccgtgcac caatcccgtg gcttcaagct ggctgcggaa
    12301 ggattcgacg ctgttcaaat cctgcgcggc gacattcggc aaaagccaaa gcatcagtgc
    12361 cgcactaatg agccacgcgg ggatggtggt gtacatcata tttttgatgt gctcaaacaa
    12421 gtcgatgccg acgatggacg cggaaatacc cgtcgtatcc gaaagcgggg acattttgtc
    12481 gccaaaaaat gcgcccgaaa caatcgcgcc cgccgtcatc gccatatcgg cctgaaacgc
    12541 cgccgccatc cccataaagg caacgccgac agtggcgcag gtggtcaggc tgctgccgat
    12601 ggacacgccg atgacggaac acagcgcgaa ggcggaaaaa taaaaataag tcggggaaat
    12661 cagtccgaaa ccgtaataca tcagcgtcgg aatcgcgccg ctcatcatca gcgcgctgac
    12721 catcagcccg atgaagaaaa acaggtaaat cgcgcccata ccctgattca acgcgcctat
    12781 catgccctgc tgcatatcgt tgtatttcaa accgcgcgcc aagccgtaca aaatcagcac
    12841 gacgatggcg gcaataatgg acatatgcgg cagccactcc aatgaaatga tggtatagcc
    12901 catcgcggca atcagagcga cgacgacggc aagtgcctca ccgcgcggca tatcgagtaa
    12961 ggatttgaaa gcgaacatga ttctttgccc gatttgatat aaagttaacg gattctaaca
    13021 caccataccg aatccgcccg acgttttcag acggcatttt agcgttttac aaaagccgga
    13081 cagaaacaaa acggcaattt tctcaattta aattaatttt tttgaaatat aattttttat
    13141 ttttttgttt gttgacatat aagactttta tggattgaag tattaaaacc tcggcacaca
    13201 cgccgcaaac accggtacgc aaaaatgccg tctgaaagat gttcagacga catttctcaa
    13261 gaaaaagatt atttgtccgc cctatatccg tacaccgcaa aaaacacgat atacagataa
    13321 cagatgacgg aaacgacaaa cgaagacatc agggtgtaag tatctgccac ccagccctgc
    13381 acgacaggaa cgaccgcacc gccgacaatc gcggtacaca gtacaccgga agccgcgttg
    13441 gtaaattttc ccaatccttt ggttgccaaa gagaaaatcg tcgggaacat aatcgagttg
    13501 aaaaaaccga tggcaagcag cgaccacatc gccacatccg cattgccgct acccgtcgcc
    13561 atcgcgacgg caagcagtac gaccgcagcc gatgcgttaa acgccaaata acggttgggc
    13621 gcgaatttcg ccatcaccgc cgaaccgagg aaacgtccga ccatcgcgcc gccccaatag
    13681 aacgacaggt aatgcgcggc agaagcatga tccagccctt tcagataacc caatacgttg
    13741 accatcaacg aaccgataga cacctccgcg ccgacatagc agaaaatgcc tgccgtaccg
    13801 aacacgagat gtttgtattg ccatacgctg gttttgccgt cgtggttgtg cgcgctttcc
    13861 tcggcggcaa ttttgcgcgc gtcgggcagc cggatcattt tcacgaaaac ggcaaggata
    13921 atcagcagcc ccgccagtcc caaataggga atctgtacgg aagaaatctg ttccgccttg
    13981 ctgacggttt gggttgcgtc cgccagaatc aggaacgcgc cgatttgcgg cgcaatggtc
    14041 gtacccaaag cgttaaacgc ctgaaccagc gtcagtgttg ccgattcctt gccgggtttc
    14101 gccagcaggg taacataagg attaccggcg acctgaagca gcgttacgcc ggaagccaaa
    14161 ataaacaacg cgcccaaaaa taccgcgtaa gaatggctac ccgcagcagg ataaaacagc
    14221 aggcatccga ccgccgtcag cagaaagccg ccgataacgc cgtttttgta gccgactttg
    14281 ccgacaaaag cccccatcgg gatggacatc accgcatagg cggtaaagaa acagaattgg
    14341 atcagcatcg cctgaacgta agacaggtcg aaaatttctt tcaaatgagg gataaggatg
    14401 tcgttcatgc aggtaataaa acccatcatg aagaacagcg tggtcaagac gaccaatggg
    14461 gacgtatgat tgttttgtga ttgcgcagac atgatttgcc tttcgtggtg tcggaatatt
    14521 acccgtttca agaaaataga aattgacatt tcttaacggc aatgcaaatc atactgatat
    14581 ttgaacattt tgcaaacttt atcggatgca ggcaatgcgc cgatctgaaa ttcaagcagc
    14641 agaaattaat attttacagt tgcttgcata gattttaata gattttaaaa aattccggcg
    14701 gacttgcaaa aaccgttccc aataaaatga aaatccgccg ccgtatccgg aaaattcgga
    14761 aatgccgcat cgtggcatca aaaccggagc ggaaagaatg tcgtatgccc gacaaaaaag
    14821 gggcatacgg ctgtgccgta ttttttgcac cgtcctccgc ccttcggcgg gggcgtgaaa
    14881 atatagtgga ttaacaaaaa tcaggacaag gcgacg
//
LOCUS       DAWHRA010000038        14807 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWHRA010000038 DAWHRA010000000
VERSION     DAWHRA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14807
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.038"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            95..1125
                     /locus_tag="WIB30_000875"
                     /pseudo
     CDS             95..1125
                     /locus_tag="WIB30_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246474.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            1153..3579
                     /gene="hupB"
                     /locus_tag="WIB30_000876"
     CDS             1153..3579
                     /gene="hupB"
                     /locus_tag="WIB30_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ3796967.1"
                     /translation="MSIPFKPVLAAAAIAQAFPAFAADLAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVKIENKGYVFSPTQSFSKYQTYVATGVARSQPDPQEWVNKSTLFKLGY
                     NFNDRNRIGWIFEDSRTDRFTNELSNLWTGTIYSPATGDYRHRQDVSYRRRSGVEYKN
                     ELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINSEVYHSFRHIRQNTAQWTAD
                     FEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFVKLYDPKIFTSNQAKITMLIESRSK
                     YKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHAG
                     FSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAETAKNF
                     ELGLAGSGKAGNFKFSGFQTRYRNFIELTYMGVSSDKPNSPNYAPISDGTALVSSPVW
                     QNQNRSSAWVKGLEFNGTWNLDSIGLPQGTHAGVNVSYIKGKAKQTNGQETPINALSP
                     WSAVYNLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLFDL
                     SAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSPGR
                     SYNFTIEAKF"
     gene            complement(4206..5840)
                     /gene="groL"
                     /locus_tag="WIB30_000877"
     CDS             complement(4206..5840)
                     /gene="groL"
                     /locus_tag="WIB30_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ3796968.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAIESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(5934..6221)
                     /locus_tag="WIB30_000878"
     CDS             complement(5934..6221)
                     /locus_tag="WIB30_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ3796969.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVE"
     gene            6449..7102
                     /locus_tag="WIB30_000879"
     CDS             6449..7102
                     /locus_tag="WIB30_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3796970.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTNSLSSCDKLDVSGFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQELKILRDWCGI"
     gene            7315..8850
                     /locus_tag="WIB30_000880"
     CDS             7315..8850
                     /locus_tag="WIB30_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ3796971.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            8847..8939
                     /locus_tag="WIB30_000881"
     CDS             8847..8939
                     /locus_tag="WIB30_000881"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ3796972.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(8975..10219)
                     /gene="lysA"
                     /locus_tag="WIB30_000882"
     CDS             complement(8975..10219)
                     /gene="lysA"
                     /locus_tag="WIB30_000882"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ3796973.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVEPKNIAPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(10230..10400)
                     /locus_tag="WIB30_000883"
     CDS             complement(10230..10400)
                     /locus_tag="WIB30_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ3796974.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            10471..10794
                     /gene="cyaY"
                     /locus_tag="WIB30_000884"
     CDS             10471..10794
                     /gene="cyaY"
                     /locus_tag="WIB30_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ3796975.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            10825..11244
                     /locus_tag="WIB30_000885"
     CDS             10825..11244
                     /locus_tag="WIB30_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ3796976.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCVNDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSREPT"
     gene            11266..11772
                     /gene="luxS"
                     /locus_tag="WIB30_000886"
     CDS             11266..11772
                     /gene="luxS"
                     /locus_tag="WIB30_000886"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ3796977.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            11918..14734
                     /gene="polA"
                     /locus_tag="WIB30_000887"
     CDS             11918..14734
                     /gene="polA"
                     /locus_tag="WIB30_000887"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ3796978.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDSMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTEEGRKVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLSECEASPWDELLQSKLIMQVHDELVLEVVETELDFVKEKLPQIMAK
                     VDGGLLDVPLVAEVGVGENWEEAH"
BASE COUNT         3541 a         4100 c         3793 g         3373 t
ORIGIN      
        1 tttttgcacc gacccacaaa accagttgac atcatataac atttaaactg ataatcaaaa
       61 ctattattaa cacatcatca agattgagta tgcgatgaaa tacaaagccc tgtccttact
      121 gccgcttgtc gctgcccttg ccgcctgtgc cggggggggg gggtagccga accgcacgtc
      181 cccgtgtcca tccccaccgc cacgccgctg cccaaaggcg aggtaacgtt atcaagcgat
      241 aacggcaaaa tcgaaaacat caacaccacc agcaccggaa gcacatccgt catttccatt
      301 caagagcggg cggttacaaa aaactatttc ggtgttgaat cacaagaaaa atcattcatc
      361 ttcaaaacac ccggcggcgc gcaatacacc ctttcatcct acgccgaccc catcgtcccc
      421 agctactcct cccccgattt caaaattccc gaccgccacg cagggcagcg gcttgcagac
      481 ggcagccgca tctttatctg ctgcagcgac tccggggcaa ccagagaagc ggaaattacc
      541 aaacaagatt atatgaaatt cggggcatgg ataggtccca acggcgaaat cgacctcttc
      601 gccggcggct tccccgtcgg caaaacaccg acatcaagca gttattacgg cagcagcacg
      661 cccgaaaccc aaggcaaagg caaaatcact tatcaggttt ggggcatccg cgtcaaagac
      721 gggcaatttg ttacctcctc ttatacgccg cccaaaggca gctcttttac cggctacacc
      781 aacacccccg tcctttcctt tatcaccgcc aatttcaaca gcaacaaact ggcaggcgaa
      841 atccgcggca acagcgatta cggaccgagt gtgaaaattg aaaacgcaac gatcagcggt
      901 ccgagctttt ccggcaatgc cacctccggc ggcaaaaccg gcaacttgga aggcaagttt
      961 tttggaaaat tcaacggcag ctacggcaac actgaaacca gcataggcgg caaaatcact
     1021 tttaaagacg accgctccct cgataccgta ttcggcggtg tgagttatgt aaaaaaactt
     1081 gatgaaaccg ccaatcgaga taccgaacac cttactaaac aataaaccat ctcatcccta
     1141 caaaggtttc ttatgtccat ccccttcaaa cccgtattgg ctgccgccgc catcgcccaa
     1201 gcgtttcccg cctttgcggc agacctcgcg ccgcagtccg cccaaacgct gaacgaaatc
     1261 accgttaccg gcacgcacaa aacccaaaaa ctcggcgaag aaaaaatccg ccgcaaaacc
     1321 ttagacaagc tcttggtcaa cgacgaacac gacctggtgc gctacgaccc cggcatttcc
     1381 gtcgtcgaag gcggcagggc gggttctaac ggctttacca tacgcggcgt ggacaaagac
     1441 cgcgtcgcca tcaacgttga cgggctggcg caggcggaaa gccgctcttc cgaagccttc
     1501 caagaattgt tcggcgcgta cggcaacttc aacgccaacc gcaacacttc cgagccggaa
     1561 aacttttccg aagtaaccat caccaaaggc gcggactcgc tcaaatccgg cagcggcgca
     1621 ttgggcggcg cagtcaatta ccaaaccaaa tccgcaagcg attatgtttc cgaagacaag
     1681 ccctaccatt tggggataaa gggcggcagc gtcggcaaaa acagccaaaa gttcagcagc
     1741 atcaccgccg ccggcaggct ctttggtttg gatgccttat tggtttatac ccgccgcttc
     1801 ggcaaagaaa ccaaaaaccg ctcgaccgag ggcgatgtca aaattgaaaa caagggatat
     1861 gttttcagcc caacccaatc attcagcaag taccagacct acgtagccac aggggttgcg
     1921 cgctcccaac ccgacccgca agaatgggta aacaaaagca ccttgttcaa gctgggctac
     1981 aatttcaacg accgcaaccg catcggctgg atttttgaag actcccgcac cgaccgtttt
     2041 accaacgagc tgtctaattt gtggacgggt acaatttact ctcccgcaac gggcgactac
     2101 cgccaccgcc aagacgtgag ctaccgccgc cgctccggcg tcgaatacaa aaacgaattg
     2161 gaacacggcc cgtgggacag cctcaagctg cgctacgaca agcagcgcat cgatatgaac
     2221 acttggactt gggacatccc gaaaaattac gatttgcgcg gcatcaacag tgaagtttac
     2281 cattcgttcc ggcatatccg ccaaaacacc gcgcaatgga ctgccgattt tgaaaaacaa
     2341 ctcgactttt ccaaagccgt ttgggcggcg caatacggct tgggcggcgg cagaggggac
     2401 aatgccaact cggattacag ctatttcgta aaactgtacg accccaaaat cttcacttcc
     2461 aaccaagcca aaatcacgat gctgatcgaa agccggtcga aatataaatt tgcctattgg
     2521 aacaatgcgt ttcacttggg cggcaacgac cgcttccgcc tgaatgcggg catacgctac
     2581 gacaaaaaca gcagcagcgc gaaagacgat ccgaaataca ccaccgccat ccgggggcag
     2641 attccccatt tgggttcgga acgcgcgcac gcgggcttca gctacggcac ggggttcgac
     2701 tggcggttta ccaagcatct gcacttgttg gcaaaataca gcaccggctt ccgcgcaccg
     2761 acttcggacg aaacctggct attgttcccg catcctgact tttaccttaa agcaaacccc
     2821 aacctgaagg cagaaaccgc caaaaacttc gagctgggac ttgccggcag cggcaaagca
     2881 ggaaacttta aattctccgg tttccaaacc cgctaccgta attttatcga actgacctac
     2941 atgggcgtat cctcagacaa gcctaacagc cctaattatg cgccgatttc cgacggtact
     3001 gcattggtca gctcgcccgt ttggcaaaac caaaaccgct cctcggcatg ggtgaaaggt
     3061 ttggaattta acggcacatg gaacctcgac agcatcggtt tgccgcaagg cacgcacgca
     3121 ggtgtcaatg tcagctacat caaaggcaag gccaaacaaa ccaacggaca ggaaacccct
     3181 attaacgccc tttcgccatg gagtgcggtt tacaatctgg gctacgacgc tccttccaaa
     3241 cgttggggca tcaacgccta cgccacgcgc accgccgcca aaaagccgtc cgacaccgtc
     3301 cacagcaacg acgacttgaa caacccgtgg ccttatgcca aacacagcaa ggcctatacg
     3361 ctgttcgacc tttccgccta cctcaacatc ggcaaacagg ttacgctccg cgccgccgcg
     3421 tacaacatta ccaacaagca gtactacact tgggaatctt tacgcagcat ccgcgagttc
     3481 ggcacggtca accgcgttga caacaaaacc cacgccggca tccaacgctt tacctcgccg
     3541 ggcaggagct acaatttcac catcgaagcg aagttctaag ctcctgatgt gccaatgccg
     3601 tctgaaaccc gatgatttgg gtttcagacg gcatttttta cggtcgcacc gccgccccct
     3661 ttcaggcttg taccaagccc aatcccgcaa ctctccgtca ttcccgcaac tctccgtcat
     3721 tcccgcaact ctccgtcatt cccgcgactc tccgtcattc ccacggaagt gggaatctag
     3781 aaacgcaaag ctgcaagaat ttatcggaaa tgactgaaac tcaacgaacc tggattcccg
     3841 cctgcgcggg aatgacgaag ctatccgcac agaaacctgc accacgtcat tcccacgaaa
     3901 gtgggaatcc agaacgtaaa atctgaagaa accgttttat ccgatacgtt tccgcaccga
     3961 cagacctaga ttcccgcttt cgcgggaatg acggcggaaa ggttgctgtt tttccgataa
     4021 attcctgccg ctcttcgttt ttgggatggc gggaaataaa acaaaagcgc gcgtatcaaa
     4081 aaacaaaaat gcaaagaacg gcgttggcaa ttttaaggtt tgcgccttag ccgcacttat
     4141 tcgcaaaaaa aaccgcacgg cgttgaccgt gcggcttgtt gtctgaaagc ttcagacggc
     4201 atttcttaca tcatgccgcc cataccgccc atgccgccca tgtcaggcat agcaggtttt
     4261 tcttcaggga tttcagcgat catgcagtct gtggtcagca tcagaccggc gatagatgcg
     4321 gcgtgttgca gcgcggaacg ggttactttg gcaggatcga gtacgcccat ttcaatcata
     4381 tcgccgtatt cgccgctgcc agcgttgtaa ccgtagttgc ctttgccttc caacactttg
     4441 ttcacgacta cgctgggttc gccgcctgcg ttggcaacga tttggcgcag cggagactca
     4501 atggcgcgca agacgatttg tacgcctgcg tcttgatcgg cattgccggt gtgcaggttt
     4561 tccagagcgg cacgggcgcg caacagggct acgccgccgc ctgcaaccac gccttcttca
     4621 acggctgcgc gggtagcatg cagcgcgtct tctacgcggt cttttttctc tttcatttcg
     4681 acttcggtag cggcaccgac tttgattact gccacgccgc ctgccagttt ggccacgcgc
     4741 tcttgcagtt tttctttgtc gtaatcgctg gttgcggttt cgatttgttg gcggatttcg
     4801 gcaacacgcg cttcgatttg ggctgcgtcg ccgaagccgt cgatgatggt ggtgttttct
     4861 ttaccgattt cgatgcgttt ggcttgaccc aagtcgtcca aagtcgcttt ttccagagac
     4921 aggccgactt cttcggaaat caccgtgccg ccggtcagga tggcgatgtc ttgcagcatc
     4981 gctttgcggc ggtcgccaaa gcccggagct ttaacggcaa cggttttcag gatgccgcga
     5041 atgttgttta ccaccaaagt cgccaaggct tcgccttcta cgtcttcagc gataatcaag
     5101 agcggacggc tggcttttgc cacttgttcc aaaacaggca acaagtcgcg gatgttgctg
     5161 atttttttgt cgaacagcaa tacaaacgga ttgtccaagc cggcgatttg tttttccgca
     5221 tcgttgatga agtaaggaga caggtagccg cggtcgaatt gcataccttc aaccacgtcc
     5281 agctcgtttt ccaaagattt gccgtcttca acggtaatca cgccttcttt gccgactttt
     5341 tccatcgctt cggcaataat cgcgccgact tgttcgtcgg agttggcgga aatcgagccg
     5401 acttgggcga tttctttaga agtgtcgcaa ggtttggcga tgtttttcag ctcttcaacc
     5461 aaagcggcga cggctttgtc gataccgcgt ttcaggtcgg tcgggttcat accggcggta
     5521 acgtatttca taccttcggc aacgatggat tgcgccagta cggtggcggt agtcgtaccg
     5581 tcgcccgcca cgtcgttggt tttggacgcg acttctttta ccatttgcgc gcccatattt
     5641 tcaaacttgt ctttcagttc gatttcttta gcgacggtta cgccgtcttt ggtaatgtgc
     5701 gggccgccga aagcgcggtc aaccaccacg ttgcggcctt tgggacccag ggttacgcgc
     5761 acggcgtttg ccagaatgtt cacgccgttt accatttttt gacggacttc attgccgaat
     5821 tgtacgtctt ttgctgccat ttcaattctc caaaaatcat taaaactgtc tgataaaacc
     5881 gtttatgccg tctgaaggcg gtttgccgtt tcagacggca tcatcattcg cacttattca
     5941 acgatgccga aaatatcttc ttcgcgcatt accaacagct cttcgccgtc ggcttttacg
     6001 gtttggccgc tgtatttgcc gaaaatgatt ttgtcgccga ctttgacatc cagcggacgg
     6061 cgcgcgccgt ctttaccgat tttgcccgcg cccacggcga tgacttcgcc catatcgggt
     6121 ttttcggcgg ccgcacccgg caaaacgata cccgatgcgg ttttttcttc agcttccaag
     6181 cgtttgacga cgacgcggtc gtgtaaagga cggatggtca tatttatgct ccgataaaat
     6241 ggtttgaaaa caatcatctg cccgaacggt tcggacagat tgaagtggaa accgggctgc
     6301 cgcctgccgg ccgcccgtat gaatcggcaa aattagggtg tgtgcgggca aattcaagtg
     6361 aggcggaaaa aatttatttc cgccgttttt tatagtggac taaatttaag gggctgtact
     6421 agattagcag atatgttacc ctcgaaatat gaagataacg cactgcaaat taaagaaaaa
     6481 agtacagaaa gaactgctcc gtttttttgt gctggaagtt accgcccgtt ctgccgccga
     6541 tattttgggt atccatccca attcggcagc actgttctac cgtaaaatcc gcacggttat
     6601 caaccatcat ttggccttgg ctgccgatga ggtttttgag ggccctgtcg agccggacga
     6661 aagcgatttc ggcggacggc gtaaaggcag acgtggtcgc ggtgcggcag gaaaagtggt
     6721 tgtcttcggc attctgaaac gcaacggacg ggtctatacc gttgtggtgg ataatgccaa
     6781 gtctgaaacg ttactccctg tcatcaaaaa gaaaatcatg ccggacagca ttgtttatac
     6841 caatagtctg agcagctgcg acaagttgga cgtgagcggt tttatccatt accgcatcaa
     6901 ccattccaag gagtttgcag accgtcaaaa ccacattaac ggcattgaga acttttggaa
     6961 tcaggcaaaa cgcgtcttgc gaaaatacaa cggaatcgat cgtaaatctt tcccgctgtt
     7021 cttgaaagaa tgcgaatttc gatttaactt cggcacaccg tctcaagagc ttaaaatcct
     7081 gcgggattgg tgtgggattt agggctaatc tagtacagcc cctaaaattt ttcgttttca
     7141 agccttcacc gcttgccatc agcgttaaat ttttttacga taagcacata gattgtaaac
     7201 aatcggccac aagccggttt gttttttcag aagacattat ccctgtcaga cgctatttct
     7261 atatatttcg cctataatgg cttgttttta ataaataatt caagaggtat caacgtgtct
     7321 gattccaaga cgaaagaacg cgccacattc ggcacgcgcc gcgcgtttat gattgccgcc
     7381 atcgggtccg ccgtcggctt gggcaatatt tggcgtttcc cctatattgc ttttgaaaac
     7441 ggcggcggcg cgttcatcct gccctatctg gtcgcgcttc tgacggcggg catcccgctg
     7501 ctgctgctcg attatgccat cggccaccgt taccgtggtt ctgcgccctt ggctttccgc
     7561 cgcctcggac gatggtttga gccggtcggc tggtggaacg tgatgaccaa tatcgtcatc
     7621 tgcatctatt acgcggtaat tatcggttgg gcggcaagct atacctatta ttcggtcaac
     7681 gccgcctggg gtgcggatcc gcagggtttt ttctttaagg acttcctgca aatggcgggc
     7741 ccggaagcct tgggtttgga ttttgtcggc aaagtcgccg gtcctttggc gggcgtgtgg
     7801 gtttttaccg ccgccattat ggcgttgggc gtgcaaaagg gcgtggcgcg cgcctcgtcg
     7861 ttctttatgc cgctgctttt ggtgatgttt ttgattatgg tcggcatttc actaaccctg
     7921 ccgggtgcgg caaagggctt ggacgcattg tttacgcccg actggtcgaa actcgccgat
     7981 tccaaggtct gggtggcggc atacgggcag attttctttt cgctttccat ctgcttcggc
     8041 attatggtta cctattcttc ttatttgaag aaaaaaaccg acttgggcgg aacggggctg
     8101 gtggtcggtt ttgccaacag cagctttgaa ctgctcgcgg gcatcggcgt gtttgccgca
     8161 ttgggcttta tggcgcaggc gggcggtaag gcggtcaacg aggttgcctc aggcggcatc
     8221 ggtttggcgt ttatcgcctt tccgaccatt atcaaccagg caccgatggg ctggctgatc
     8281 ggcatattgt ttttcggttc gctggtattc gccggcgtta cgtcgatgat ttccatcctt
     8341 gaagtgattg tggcggcgat tcaggacaag ctgaacatcg ggcgcgtcaa cgccacgctg
     8401 ctggtctgca ttccgatggg cattgtttcc acgctgctgt tcggtacggc gacggggctg
     8461 ccggttttgg acgtgatgga caaattcgtc aacacctacg gcattgttgc cgccggcttt
     8521 gtttatgttg ccgccatcat catcagcggc aggctgccgg aattacgcaa gcacctgaac
     8581 gctttgtcct ccatccgcat cggcggcttg tggacggtct gcgtcgtggt taccgtcgtg
     8641 atgctcggct atatgctgtt taaagatacc agcggcctga tggagaaaaa ttacgaaggt
     8701 tatccggatg gtttcctcag tattttcggc tgggggatgt cggcggcgtt ggtcgtgttc
     8761 gggctgctgc tgtcgttgct gccttggaaa cacggtcagg atttcaacgt caaagacgaa
     8821 cacgaacatg aacaaggaga agaaaaatga gtacttccgc cattgtgatg atgattgtct
     8881 caatcgtgat aatctgggga gggctgctgc tttccctgtt aaggctgccg aacgagtaag
     8941 cctttagagc gttaaaaatg ccgtctgaac cgcttcagac ggcattttga tgttcggctt
     9001 gcaggcaggc gagttcgttt gccatttgct gttctaaggt ttcgcgtcgg cggatgagtt
     9061 tgtatccgcc accgttaacc aacacttccg ccgcacggtt gcgcgcgttg taattgctcg
     9121 ccatactggc tccgtatgcg cccgcgctgc ggataagcag caaatcgcct tcttcgcagg
     9181 cgatggtgcg gtctttgccg aggaagtcgc cggtttcaca aatcgggccg acgatgttgg
     9241 cagtcagcgg cgcgatgttt tttggttcta ccgcttcgat gtgatgatag gcatcatata
     9301 gggcggggcg cattaaatcg ttcatcgccg catcgaccat cacaaagttt ttctcttcgc
     9361 cgtatttgac gaattcgaca cgcgtcaaca acgcgcctgc gttgccgacc aagctgcggc
     9421 cgggctcaag aatgagtttc agacggcgtg tgccgatcag tttttgaacc gcttgggcat
     9481 acgcgcccaa atcaggcact ttttcgtttt ggtaaacaat gccgacgccg ccgcctaagt
     9541 ccaaatgttc caaaacaatg ccttcggcgg caagcgcgtc aaccaaaatc aaaatgcgtt
     9601 cgcaggcttc gaccagcggg cttaagtcgg ttagttggga gccgatgtgg cagtcgatgc
     9661 cgatgatttt caaattgggc tgttgtgcgg catggcggta ggcttcgagc gcgtcggcgt
     9721 aggcgatacc gaatttgttg gctttcaaac ctgtggagat gtaaggatgg gttttcgcat
     9781 cgacatcggg attgacgcgc agggagacgg gcgcggtttt acccaaacgc gcggcaactt
     9841 tctgaatacg gtcgatttcg gggatgcttt ccatattgaa gcatttcacg cctgcattca
     9901 gcgcgaactc gatttccgcc tcgcttttgc ctacgcctga aaatatggtt tttgccgcgt
     9961 cgccgcctgc cgccaaaacg cgtgccaatt cgccgccgga cacaatgtca aaaccgctcc
    10021 ccaaagaagc aaagtgtttg atgatgctca ggttgccatt ggctttaacc gcatagcaga
    10081 cgagcgggga gagctcggca aaggcagttt ggtagtgttc aaatgcttcg gtcagcgcgg
    10141 attggctgta cacataaagc ggtgtgccga atgcttcggc aaggcggggg taggggactt
    10201 gttcgcaaaa tagggtcata ttttcgtttt catttttggg ttggaggggc ggattgcggt
    10261 ttgccttgaa gctgcaaacc ggtttggatt acgccgaaac gcgccttgtc gccttctttg
    10321 ggcaggtaga ggtcgccttt gtaaccgcag gccgagagca ggagggcggt tgccgccgca
    10381 aaaaatacgc cgtatttcat cggtaaactt ccttcataag cgcgaatgtg gcaagattcg
    10441 gcatcttaaa caaaaaacac gcaaaaagct atgatgaccg aaagcgagtt tatccgcgcg
    10501 agcgaagcat tatttgaaca catcgaagac caaatcgacg aaaacggctg ggatttcgac
    10561 tgccggtttg ccggaaacgt cctgaccatc gaagccggag acggcacgca aatcatcgtc
    10621 aaccgccaca cgcccaacca agaattgtgg attgccgcaa aaagcggcgg ctaccatttc
    10681 gccgaacaaa acggcaaatg gctggcaacg cgcgacagcc gcgattttta tgatgttttg
    10741 aacgaagcac tgagcgcggc ttcgggcgaa gcggttgaga ttgaggaatt gtgattcaat
    10801 ttgatataaa aagattgtta cccaatggca caattacctc tatatcggac tgccgaaatc
    10861 ggaaatttta ctgtcggcac gcctaaagtt ttagaatcat tttccaaaca tatcccttat
    10921 ggtgtcgtct ttgaagacga cggcgacacg ggctacttct atgctgcttc gcaagacggg
    10981 attttagatg ccttgcacat ctacaatgtc gaagatgtat ccgacaaaca tatccccaat
    11041 catgtcttga ttttatggga tgatgcctgc accatagccg cattgtgtgt caacgactac
    11101 attcatgccg tctatgattt tgtcgaacag gcaggatatt gccgcaacgg cttccctgaa
    11161 gcaggcggcg aatgggtgaa agtcgaaaac cgcgtcttgg atgatgaatt gctggacaaa
    11221 atcctatccc gagaacctac ataaccctca caaaaggata cccaaatgcc cctactagac
    11281 agtttcaaag tcgatcacac ccgtatgcat gcccccgccg tacgcgtggc gaaaaccatg
    11341 accacgccca aaggcgacac cattaccgtg ttcgacctgc gcttttgcat tcccaacaaa
    11401 gaaatcctgc ctgaaaaagg catccacacg ctggagcatt tgttcgcagg ttttatgcgc
    11461 gaccacttga acggaaacgg cgtggaaatc atcgacattt ccccgatggg ctgccgcacc
    11521 ggtttttata tgagccttat cggcacgcct tccgaacagc aggtcgccga tgcgtggctg
    11581 gcttcgatgc aggatgtttt gaatgtcaaa gaccaaagca aaatccccga gttgaacgaa
    11641 taccaatgcg gcacttatca aatgcactcg ctcgccgaag cgcaacaaat cgcgcaaaac
    11701 gtgttggcgc gcaaagtggc ggtgaacaaa aatgaagagc tgacgctgga tgaagggctg
    11761 ctgaacgcct aatccgccaa aaatgccgtc tgaacaaggg tttcagacgg catttgcctt
    11821 ttccgttata atccggggtt gtgcgggggc gggttttaag ccggcatcgt ccttccctat
    11881 ttttttctgt cccttatcgg ttttaagcgg gttttttatg tccaacagac ctacactcct
    11941 cctcgttgac ggatcgtcct acctctaccg cgcgtatcat gcgatggggc aaaacctgac
    12001 cgcccccgac ggcgcgccga cgggcgcgct gtatggcgta ttgaatatgt tgcgccgttt
    12061 gcggtcggaa tatccgcacg attattgcgc ggtggttttt gatgcgaaag gcaaaaattt
    12121 ccgccatcaa atgtttgaag aatacaaggc gacgcgcccg ccgatgcccg acgatttgcg
    12181 cccgcaggcg gaagcactgc cggatttagt gcgcctgaca ggctggccgg tattggtgat
    12241 tgggcaggtg gaggcggacg atgtgatcgg cacgctggcg aaacaggggg cggaacatgg
    12301 tttgcgagtc attgtttcga ccggcgataa ggacatggcg cagttggtgg atgagcgcgt
    12361 tacgctggtg aacacgatga gcagcgaaac gctggacatt gaaggcgtga aggcaaaatt
    12421 cggcgtgcgc cccgaccaaa tccgcgatta tctcgcgctg atgggcgaca aggtggacaa
    12481 cgtgccgggc gtggaaaaat gcggcccgaa aacggcggtg aagtggctgg aagcctacgg
    12541 ctcgttgcaa ggcgtgatag aacacgcttc ggaaatcaag ggcaaagtgg gcgaaaacct
    12601 gcaagccgcg ctgccccaac tgccgctgtc gtatgatttg gtcacgatta aaaccgatgt
    12661 ggacttgcac gccgagcttt cagacggcat cgaaagcctg cgccgtacta cgccgaaatg
    12721 ggcgcagctg gttgtcgatt tcaaacgctg gggcttccgc acctggctga aagaagcgga
    12781 atcaaacatg aataccggct cgaccgatga tttgttcggc agcgacagca tcggcgagca
    12841 ggcggctttg aatgcggaaa tgccgtttga aaaacaagcc gaaaaagcca ccgcccccga
    12901 aaaactggat tatcaagccg ttaccaccga agcgcagttt gccgctttgt tggacaaact
    12961 gtcgcgggcg gacacaatcg gcatcgatac ggaaaccacg tcattagacg cgatgaacgc
    13021 ctcgctggtc ggcatcagca tcgctttcca agcaggcgaa gcggtttaca tccccgtagg
    13081 acacagcctg accgccgcgc ccgaacagct tgatttgcaa gacgtgttag gccgtctgaa
    13141 accgcatttg gaaaaccccg ccctgaaaaa aatcgggcaa aacctcaaat acgaccaaca
    13201 cgttttcgcc aactacggca tcgccctgaa cggcattgcc ggcgattcca tgctcgcttc
    13261 ctacatcatc gagagccatc tcggacacgg cttggacgaa ttgtccggac gctggctcgg
    13321 cttggaaacc attacctacg aatcgctgtg cggcaaaggc gcgaagcaaa tcggttttgc
    13381 cgatgtcgcc atcgggcagg cgaccgaata cgccgcccaa gacgccgatt tcgccctgcg
    13441 cctcgaagcg cacctgcgcg cgcaaatgga cgaaaaacag cttgaaatgt atgaaaaaat
    13501 ggagctgccc gtcgcgcagg tattgtttga aatggaacgc aacggcgtgc aaatcgaccg
    13561 cgccgaactc gcccgccaaa gcgcggaact cggcgccgag ctgatgaagc tcgaacagga
    13621 agcctatgcc gccgcaggcc agccgttcaa cctcaattcg cccaaacagc tgcaagaaat
    13681 cctgttcgac aaaatgggca tccccaccaa aggcctgaaa aaaaccgcca aaggcggcat
    13741 ttccaccaac gaagccgtgc tcgaacagct cgcgcccgac taccccctgc ctaaaatcat
    13801 cctgcaaaac cgcagcctgg cgaagctcaa atccacctac accgacaaac tacccgaaat
    13861 gatttccccc aaggacggcc gcgtgcatac cacctacgcc caagccgtcg ccattaccgg
    13921 ccgcctcgcc agcaacaacc ccaacctgca aaatatcccc atccgtaccg aagaagggcg
    13981 taaagtccgc cgcgccttta ccgcaccgca aggcagcgtc atcgtttccg ccgactattc
    14041 ccaaatcgag ctgcgcatta tggcgcacct ctccggcgac aaaaccctga ttgccgcgtt
    14101 ccaaaacggc gaagacgtac accgccgcac cgccgccgaa gtgttcggca ctgcgcccga
    14161 aaacgtctcg tccgagcaac gccgctatgc caaaaccatc aacttcggct taatttacgg
    14221 tatggggcaa tacggtttgg caaaatcatt gggcatcgac aacctttccg ccaaaaactt
    14281 tatcgaccgc tacttcgccc gctaccccgg cgtcgccgaa tacatgcagc gcaccaaaga
    14341 acaagccgcc gcccaaggct acgtcgaaac cctgttcggc agaaggctct acctgcccga
    14401 catccgcaac aaaaacgcca acgcccgcgc cggagccgaa cgcgctgcca tcaacgcccc
    14461 catgcagggc accgcctccg acctcatcaa acgcgccatg atagacgtgt cccgctggct
    14521 ttcagagtgc gaagcctccc cgtgggacga actcttacaa agcaaactga ttatgcaggt
    14581 gcatgacgaa ctggtgctgg aagtcgttga aaccgaactg gattttgtca aagaaaaact
    14641 gccgcagatt atggcgaaag tggacggcgg attattggat gtaccgctgg tggctgaggt
    14701 tggcgtaggg gagaattggg aagaggcaca ttgattgaaa ggtgttatat gctatcttta
    14761 tttaaataaa atttaatttt tggtatattt tttctaaatg ttcctat
//
LOCUS       DAWHRA010000039        14705 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWHRA010000039 DAWHRA010000000
VERSION     DAWHRA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.039"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(389..465)
                     /locus_tag="WIB30_000888"
     tRNA            complement(389..465)
                     /locus_tag="WIB30_000888"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(429..431),aa:Asp,seq:gtc)
     gene            complement(498..573)
                     /locus_tag="WIB30_000889"
     tRNA            complement(498..573)
                     /locus_tag="WIB30_000889"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(538..540),aa:Val,seq:tac)
     gene            complement(643..2619)
                     /locus_tag="WIB30_000890"
     CDS             complement(643..2619)
                     /locus_tag="WIB30_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ3796979.1"
                     /translation="MNEFSLAPIVIVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGITMLSVM
                     GILMLTGVPFNWAFSVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2768..3607
                     /locus_tag="WIB30_000891"
     CDS             2768..3607
                     /locus_tag="WIB30_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ3796980.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3807..4838
                     /gene="gap"
                     /locus_tag="WIB30_000892"
     CDS             3807..4838
                     /gene="gap"
                     /locus_tag="WIB30_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ3796981.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGKLDGLAIRVPTINVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(5085..5810)
                     /locus_tag="WIB30_000893"
     CDS             complement(5085..5810)
                     /locus_tag="WIB30_000893"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ3796982.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDTGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNTVPLKEEIGRREVACKGL"
     gene            complement(5880..6314)
                     /gene="fur"
                     /locus_tag="WIB30_000894"
     CDS             complement(5880..6314)
                     /gene="fur"
                     /locus_tag="WIB30_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ3796983.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETSKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            6533..6910
                     /locus_tag="WIB30_000895"
     CDS             6533..6910
                     /locus_tag="WIB30_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ3796984.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            6920..7729
                     /gene="dapB"
                     /locus_tag="WIB30_000896"
     CDS             6920..7729
                     /gene="dapB"
                     /locus_tag="WIB30_000896"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ3796985.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDTPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7843..8253)
                     /locus_tag="WIB30_000897"
     CDS             complement(7843..8253)
                     /locus_tag="WIB30_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796986.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFIYGYATKSQDKDIDNILELGELKQDDEILDYGGT
                     LELIGGRCDLLTGFSIDIVCREIELEFLDQKSFN"
     gene            complement(8255..8500)
                     /locus_tag="WIB30_000898"
     CDS             complement(8255..8500)
                     /locus_tag="WIB30_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796987.1"
                     /translation="MDYLLEAARITKEGGEIVINGTSNNKYLRGIPNEGELARVGLRL
                     KYNGPLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(8508..9680)
                     /gene="lpxB"
                     /locus_tag="WIB30_000899"
     CDS             complement(8508..9680)
                     /gene="lpxB"
                     /locus_tag="WIB30_000899"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ3796988.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFAEVVKRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAERLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATAATKRRLAEILQRSEFAGLPLTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRVLHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9728..10720)
                     /locus_tag="WIB30_000900"
     CDS             complement(9728..10720)
                     /locus_tag="WIB30_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ3796989.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAERKMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            11214..13973
                     /locus_tag="WIB30_000901"
     CDS             11214..13973
                     /locus_tag="WIB30_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ3796990.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         3736 a         4719 c         3401 g         2849 t
ORIGIN      
        1 tttgttaatc cactataaaa ggtcttttct aaaatatgca gaaattcgaa tatcgaattt
       61 gccttatgat tacggttttc tgtcttatct gccttaaaac gccggtattc cgtttgaacc
      121 aaattatatt tgccgaaacg ctcaaaaatc ccccttacct gcccgacaga catcaagcct
      181 tcgttgttgt agctgagaaa atatattgaa agtccgcatc cctaatcaaa gcctcaaata
      241 ccggaatcac atgcgatttt gagcaagtcc gcgctaaaac acatcgtaca gcgcaaaacc
      301 ggatgggaat ggcaaaatcc ggaaacaaac atttttcaaa gcatacaaac aaaaaaatac
      361 caaccggagg ttggtattcc ttaacttttg gcgcggcgga cggggctcga acccgcgacc
      421 cccggcgtga caggccggta ctctaaccaa ctgagctacc accgcgcatc cattgtttgc
      481 aacaatgaaa gaaaacttgg tgggtgatga cggagtcgaa ccgccgacat tctgcttgta
      541 aggcagacgc tctaccaact gagctaatca cccttgcgat ttgcgaaagc gttattaaac
      601 caaataaccg gaaatggcgc aagccttatt tcggcatttc atttatattc cctgcaaact
      661 ccagttttca aaagagataa tttcttcttt tttgcctgcg accaccaaca cgtcaccgcc
      721 ttccaatgta aacgaggcat ccgggttttc aatccggccg gtgcggcggc ggacgaacaa
      781 gagtttgatg ccgtaagccg ccatcggaag cgtgccgact gttttgccga cggcgtatgc
      841 ttctgcagcc aaaggaaagg cgtgacggac ggtttcgccg ttttcgccga atcctgcctc
      901 atcatcacta ccgacaaaca gtccctccaa cgcggcataa cggctgtggc ggatatttgc
      961 catcgtctga tagacgtgcc gatacgacgc gccgttgcct aacattgcat agcctgccag
     1021 cacgagtccg gtttctttgg tgtccgacac ggcttcttcc gcacctatat cggtaaacgt
     1081 tttcacataa tcgtcgttgg tggcgcgcac atatacgggc atattgggat acatggacag
     1141 cacattgtct aaaacgtgtt gcgtttcgtg catattgttg agcgtaacca ccaccatttt
     1201 cgcccgtccc agaccggcgg cttccaatac ttccctgcgt ttcgcatcgc cgaacgacac
     1261 cggttcgccc gcacttctgg caacctgcac ccgcgcaatg tccaagtcga gcgcgaaata
     1321 cggaatatcc tcttgggcaa ggacgcgtcc gaccgtctgc ccgcccctgc cgaagccgac
     1381 aatcagcaca tggtcggact tgctcatggt ttctaccagc atactgtgca gatcgagcga
     1441 cttcatgtcc cagcttgact tgaccaaacg cccgaccagc gcatcgctgc cgcccaagag
     1501 gaagggcgcg ataatcatcg acagcagaac cgccgccgtc gccgcctgtt cccattctgg
     1561 cgaaaccata tcaagctgcc cggcaatggc cagcatcacg aagccgaact cgccgccctg
     1621 cgcgagatac aaagccgttt tgaggctgtc gccgaccgaa tgtttcattt tgaaggcaat
     1681 ggcaaacaca accagtgcct tcaacaccag cagcattgcc aacagcatca atacctgccg
     1741 ccagccgccg atcaatgcct gaatgtccag cttcatgccg accgtgataa agaaaaagcc
     1801 gagcaaaata tcgcggaacg ggcggatgtc gtcttcgact tggaaacggt attccgtttc
     1861 cgaaagcagc atgccggcaa cgaatgcgcc caacgccata gacaaacctt ccagctcagt
     1921 cagataagcc acacccaagg ttaccagcag cacattgatc ataaagagtt cggacgattt
     1981 gcgttttgcc accatcctga accatcgcga cataattttg ctgccgacga aaaacagcag
     2041 ccccagcgtc agcagcattt ttgcaaacgc caaacccaag gccgcccaaa tatttccgtc
     2101 ccctccgccc gccagcgcgg gaatcagaat catcagcggc acgacggcga tgtcctgcat
     2161 cagcagcacg cccatcgcca tctgaccgtg cggctgcccc aattccgtct tttccgacaa
     2221 aatccggctc acaatcgccg tggacgacat cgccaacgcg cccgacacgg aaaacgccca
     2281 attgaacggc acgcccgtca gcatcagtat gcccattacc gacagcatcg taatgccgac
     2341 ctgcaaaccg cccagaccga acaccagccg cctcatcgcc ctcaacttgg gcagcgagaa
     2401 ctccaaaccg atgctgaaca tcaggaacac aatcccgatt tcgcccaaat aatccgtcgc
     2461 atggcttttc ggaatcaggc tgagcatacc gggccccgcc aaaaagccca ccagcaggta
     2521 gcccagcatg gagggaatgt tgaacttgcg gcacaggatc accgtaatga ccgacaccag
     2581 caaaacaatc acaatagggg caagcgaaaa ttcgttcata gaccgtccga acaggaaaat
     2641 acagaaaaat gccgtctgaa acggcatacg ccgccgtatt ataacaaaac accgcgcacc
     2701 atccgaaacg ggcgcggcat acaattctgc taaaatacgc cctttcgatt ttgagccgca
     2761 caccgacatg accgccacag ccgccgccat agaccgcctg cttccccaaa cccaatgccg
     2821 cgaatgcggc tacgacggct gcctgcccta cgcacaggca gtcgcaacag gcgaagcgta
     2881 caacctctgc gccccgggcg gagaagccgt cattcgggac atttccgccc tgctcggcaa
     2941 accccttgtc gcacctgcca aaacccaagc caaagcgctc gcccggatag acgaaaccgc
     3001 ctgtatcggc tgcaccgcct gcatccgcgc ctgccctgcc gatgccatta tgggtgcggg
     3061 caaacttatg cacaccgtca tcgccgacga atgcaccggc tgcggactct gcgtcgcccc
     3121 ctgccccgtc gactgcatcc atatgcagcc cgttgccgac accgtcctgc cccgcgcgcg
     3181 ccgcttcagc ctgtccgccg acagccgttt tgccgccgcc gaacacgcgc gtgcgcgcta
     3241 cctcaaacgc aacgaacgca aacagcgcga agccgacgaa cgcaaggcca tgcttgccga
     3301 acgcgaagcc gccgtccgca acgcgcgtcc gcaaacaccc gacacaccga aaaaaccggc
     3361 gtttaaccct gccgacctca tcgccaaagc catggcaaaa gcgcaaaccc aacaagaccg
     3421 cctcgccgcc gccgacaacc gcaaagacta tcaggcgaaa cagatagccg aagcccgcga
     3481 acgcgccgag ttgcgccgcg cccaacgcga tatgaaatac ggcagcgaca gcgaaaaagc
     3541 cgccgccctc gaatacctca aacaatacaa agccaaacag gaagccgcac agaataccgc
     3601 ctcctgaccc ttccctgata tgccgtctga agccgcttca gacggcattt tatcaagctc
     3661 tccgtccgcc gcacccgtgc cgtccgcatc ttaccgccca cccttccggc cgcgccgttt
     3721 tcaataaaat attaattaca cgccactaca aatttgctat aatccgcccc gaaaatctac
     3781 ccaacccctc aacaaaggaa cgaaccatgg gcatcaaagt cgccatcaac ggctacggac
     3841 gcatcggccg ccaggttttg cgcgccatct acgattatca gattcaagac caactccaaa
     3901 tcgtcgccgt caacgccagc ggcagccttg aaaccaacgc ccatctgacc aaattcgaca
     3961 ccgtacacgg acgctttgaa gccgacgtat cccacgacgg cggcaacctc atcgtcaacg
     4021 gcgacaaaat ccccttcttc tcgacccgca accctgccga actgccgtgg aaagaactcg
     4081 gtgtcgattt ggtcatggaa tgcaccggcg cgttcaccag caaagaaaaa gccaaagttc
     4141 atctggaaag cggcgcaaaa aaagtcctca tttccgcacc gggcggcgac gatgtcgatg
     4201 caaccgtcgt gtacggcgtg aacgacagcg tcctgaccgc cgacatgacc gtcgtttcca
     4261 acgcttcctg caccaccaac tgcctctcgc cggttgccaa agtgttgagc gaaagcgtcg
     4321 gcatcgtcaa aggcgcgatg accaccatcc acgcgctgac caacgaccaa accgttaccg
     4381 acgtgcgcca caaagacctg cgccgcgccc gcagcggcgt ggaaaacatg attccgacca
     4441 aaaccggcgc ggcaaaagcc gtcggactgg tactgcccga attgaaaggc aaactcgacg
     4501 gactcgccat ccgcgtgccg accatcaacg tatcattggt agatttgagc ttccaagccg
     4561 cgcgcgacac cacagtcgaa gaaatcaacg cactgatgaa agccgcctcg gaagcgggtg
     4621 cgctcaaagg cgttttaggc tacaacaccc tgccgcttgt ttctatggac ttcaaccaca
     4681 ccaccgaagc cagccacttc gacgccacac tgaccaaagt cgttgacggc aacatgatta
     4741 aagtcttcgc atggtatgac aacgaatggg gcttcagctg ccaaatgctg aacaccgcac
     4801 gccgcatgtt cggacttgaa gtgcgcccgc tcaaataagc aacaaaccgt caaacaaaat
     4861 gccgtctgaa acccgatgtt ttcaagtttc agacggcatt tttcaccgtg attttacccg
     4921 gctgtcgtca tttccaattt tatagtggat taacaaaaac cagtacggcg ttgcctcgcc
     4981 ttgccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
     5041 ctataatcca ccatatttga acctgaccga agcataagcc gccatcaaag ccccttgcac
     5101 gccacttcgc gccgcccgat ttcctctttc aacggcacgg tgttcaacat ccgcagacgt
     5161 tcggcaaaat ccgcaaacgg cagcagctcc gaaccgaaac ggcgcatatg ttccgtatcc
     5221 tgctggcagt ctatcagttc cacgcccaaa tccgccaaaa acggcacggc gcaggcaaac
     5281 gcgattttcg acgcatccgg ttgcaacgca aacattgatt cgccataaaa caccctgccg
     5341 atttgcacgc cgtaaaagcc gcccgccaac ctcgtttcac cgctttcatc gggataatgg
     5401 cactcgaacg aatgcgcgtg tcccatttcg tgcagcttca aatatgccgt ctgaaactcg
     5461 ggcgcaatcc aagttccgtc ctgattcggg cgcgccgctg ccgcacaatg cgcgaccact
     5521 tccgcaaaac agccgttgac cgcaacccga tagctgccgt tgcgcagcgt tttcgccagc
     5581 gagcgcggaa cgtgcagacg ttcgggaaca atcaccgcac gcggaccgac cgcataccaa
     5641 aaaaaccaac cgtcccgaga aaaccacgga aacacgccgt tccgatacgc ctcaagcagc
     5701 cgccccgtat ccaaatcgcc gctcacgccg accagcccgt cgcaccgggc caaagcatag
     5761 gcaggatcgg gaaaggcata attgtcaggg gcaagcagcg gaatacgcat ggcagttctc
     5821 caaaaacaca gacggcatcc aaaatcatga atgccgtctg aacaacaaac cgtccggatt
     5881 taacgtttgc ccttggcctg acagtcgctg cacacgccgt acatataaag cgcgtgatcg
     5941 acgatgcggt agccgttttc ttccgcgatt ttgtcttgca gggcttcgat ttcgggattg
     6001 tggaattccg ttacctcgcc gcacttcacg cagacgatat ggtcgtggtg gtcgcccttg
     6061 tccaactcat aaaccgcctt gctcgtttca aaatggtggc gttgcagaat acccgcctgc
     6121 tcaaactggg tcagcacacg gtaaatcgtc gccacaccga tttccacacc ctcttccaac
     6181 aaaatgcggt acacatcttc cgcactcaaa tgctcttccg catgcgtctc gaacaaatcc
     6241 aaaatcttca aacgcgggcc ggtaaccttc agaccgctgt ctttcagttg tgcaatattg
     6301 ttgaattttt ccataatatt caatacccct gtaaaacaat agacgttata atacgcaatt
     6361 tcggcctgtt tgcccactat cgcaccatag cagtttgcaa tagtaaaacc gcaccggcgg
     6421 ccgatgcgcg gacagggcgg ataccgctta atcgtatgat tatcgttcgc ttttataaaa
     6481 tattcaagca gtgttacact acccatcccg atttgcacag aaaggcattt ccgtgaacaa
     6541 aaccctcatc ctcgcccttt ccgccctcct cggccttgcc gcgtgcagtg ccgaacgcgt
     6601 ttcactgttc ccctcgtaca aactcaaaat catacagggc aacgaactcg aaccgcgcgc
     6661 cgttgccgcc ctccgccccg gcatgaccaa agaccaagtc ctgctcctgc tcggcagccc
     6721 catactgcgc gacgcattcc ataccgaccg ctgggactat accttcaaca cctcccgcaa
     6781 cggcatcatc aaagaacgca gcaatctgac cgtctatttt gaaaacggcg tactcgtccg
     6841 caccgaaggc gacgtcctgc aaaacgctgc cgaagccctc aaagaccgcc aaaacacaga
     6901 caaaccataa ggaacacaca tgacaccgct caaaatcgcc atcgccggcg caaacggccg
     6961 tatgggacgc gtattggttg aagccgtcaa caaccatccc gacaccgtcc tttccggcgc
     7021 gctcgaacac tcgggctcag aagccctcgg gctggacgca ggctacgccg tcggactcaa
     7081 aaccggcatc gccatttcag acgacgttga cgccgttctc gcacaaagcg acgtactcat
     7141 cgacttcacc cgccccgagc ccaccctcaa acacctgcaa aaatgcgttg aaaaacaagt
     7201 caacatcatc atcggcacaa caggcttcga cgatgcgggc aaagccgcta tccacactgc
     7261 cgccgaacaa acaggcatcg ttttcgccgc caacttcagc gtcggcgtca acctcacctt
     7321 ccacatcctc gacaccgtcg cacgcgtact aaacgaaggc tacgacatcg aaatcattga
     7381 aggccaccac cgccacaaag tcgatacccc cagcggcacc gcgttacgca tgggtgaagt
     7441 catcgccggc gcgctcggcc gcgacctcaa acaatgcgcc gtttacggcc gtgaaggcca
     7501 caccggtccg cgcgatccgt ccaccatcgg ctttgccacc gtccgcgcag gcgacatcgt
     7561 cggcgaccac accgccctct tcgccaccga cggcgaacgc gtggaaatca cccacaaagc
     7621 cggcagccgc atgacctttg ccgccggtgc cgtccgcgcc gcagtttggg taaacggcaa
     7681 aacgggtttg tacgatatgc aggacgtact cgggctgaac aaccgttaac ccccatacaa
     7741 aatgccgtct gaaaagatat tgttcagacg gcattttgcc aacaggctcc gtatcggcat
     7801 atcaataaag ttgcctgcgc agcatgacaa gctacggctc gtttaattga aactcttctg
     7861 atctaaaaat tctaactcta tttcccggca aactatatct atactaaaac cagttagaag
     7921 atcacatcta ccgcctatca attccagcgt acctccataa tccaagattt catcatcctg
     7981 ctttaattcg ccaagctcca atatattatc gatatcttta tcttggctct ttgtagcgta
     8041 tccatagata aatttcagat agctgaactg cttgaattta atttttaccc agtctgtata
     8101 atttggtgta ggaatctctt gaggaaagtc cggataatcc acattctgga cgatgatatg
     8161 gaattcatcg tttttcaaag tatagtttaa tagagtgccc tcatatatat acagaaattg
     8221 ataatctttt tttggaatgg tataagttgt catattattt taccttttca aaaacaatga
     8281 gtttaagatt ttttgagtcc aagttggttc tactactctt atgaaatttc agttgtttaa
     8341 actcttcctt taacggccca ttatatttta accttaatcc cactcgagcc agttctcctt
     8401 catttggaat tcctcttaaa tacttattgt ttgatgtgcc attaatcaca atttctcccc
     8461 cttcttttgt aatacgtgct gcttcaagaa gataatccat taaccgctca acatcccgcc
     8521 tcttccaaaa ccgcgcgcgc ggccaaatcc gccgtatctt ttttcaacag caggtgcaac
     8581 accctgaaat cctgttgcag cgcggcaacc ttatcggggt gttcgtacca gtccgccaac
     8641 gccgccgcca gtttttccgg tacggcatca tgttgaagca gctccggcac agcctcctta
     8701 cccaacagga tattcggcag gccaacatgc ggcactttga ttttgcgttt cacataagca
     8761 taagtcagcg gcgaaatctt gtagctgatg accatcggac gcttgcacag cgccacttcc
     8821 aaagtcgccg taccgctcgt taccagcacc gcatccgccg ccctacacac cgtttcagac
     8881 tgtctgtcgg ttaccgtcag cggtaatccg gcaaactccg aacgctgcaa aatttccgcc
     8941 aaacgccgct tcgtcgccgc cgttgcggca ggcagcagga agcgtgcggc aggatagcgt
     9001 tccaacaaca ataatgccgt ctgaaaaaac accggcgcca tatagtcgat ttcgctgacg
     9061 cggctgccgg gcagcagggc gaatacgggg atgccggcat ccacgcccaa agttttccgc
     9121 gccgtttcac ggtcgtcttc caagggcata agctgcgcca tcggatgacc gacaaactcc
     9181 gcacgtccgc ccgcatcgag ataaagctgc ggctccatcg ggaacaggca cagcacgcgg
     9241 ttgacctgat gcacgatttt gcccacgcgt tcccgccgcc acgcccacac cgacggactg
     9301 acataatgca gggtcggaat gcccgcccgt ttgagccgct ccgccacacc cagattaaaa
     9361 tcgggcgcat cgatgccgac aaacacatca ggtttcaacg acagcaaatc ccgtaccagc
     9421 cccctgcgta tccgtaaaat ttccggcagg cgttttacca cctcggcaaa gccgcgcacc
     9481 gccagtcgct cctgatcata aaggctctcg aaaccttccg ccttcatcag ttcgccgccg
     9541 ataccgacaa aacgcgcctg cgggcaacgc tttcggatgg cgcgtatcag gtgcgccccc
     9601 aacaggtcgc ccgacgcttc gccgacactg acggcaatca aagggctttt tttatcaacc
     9661 atattcgtct gtccccacat actttcacgt cccgatgccg tctgaaggct tcagacggca
     9721 tcagggttca cattttcgtc ccgttttcca acatcaccgc aaactgcgcc aagtccggcg
     9781 gcagctccgc cttcaacacc agcggctcac ccgtgagcgg atggttcaag tgcagctcgg
     9841 acgcgtgcaa aaacatccgc ttcaaaccca acttctgcaa acgacggttc gcctgataat
     9901 cgccgtagcg ttcgtcgccc gcaatcggac agccttgaga ttgcaggtgg acgcggattt
     9961 ggtgcgtgcg ccccgttttc aacgtcgccc gcaccaaagt caggtgcgac agcccgacac
    10021 cgtgcaaaat gccgtctgaa aaacggctta acacacggaa caccgtatgc gccgactgcc
    10081 cgtccgcact gacgcgcacc atcttttcgc cttgtgcgcc ggtatatttg aacaggggca
    10141 gtttgacatg gaaattgtcg tccggcagtt tgcccacccc cagcgcaagg tagatttttt
    10201 tggggtggtc gttacggatg gcttcgtgaa gtttgacgag cgcgctgcgt ttcttcgcca
    10261 ccatcaacaa gccgctcgta tccttgtcca aacgatgaac caactccaaa tacttcgcct
    10321 ccggacgggc gcggcgcaac tgttcgataa cgccgaaact cacgccgctg ccgccgtgga
    10381 cggcaacgcc ggacggtttg tcgatgacca aaagcgcatc gtcttcgtaa acaacgtcaa
    10441 acgcacgcgc cggtacggcg gcacgccttt cagacggcat cttgcgctcc gccacgcgca
    10501 caggcggaat ccgcaccgta tccccctccg caatacggct gtcgggtttg cagcgtttct
    10561 tgttcaaccg cacctcgccg gcgcggataa tgcggtggat atggctcttg ggaacaccct
    10621 tgaggatttt tatcagatag ttatcaaggc gttgacccgc ctcatgttcg gcaaccccta
    10681 tcaagctgac cgaatctttg cttatttcgt gcgttttcat ctataatccg aacatccgtt
    10741 tcagcaaaaa gcgcgcccgc cgcgcccccc tgaaacggtt tactttaaaa cggaatttta
    10801 tattaaaaac gcactccgca aagcattttc cctgcgcctg ttcccagccg gcaggcgcag
    10861 aacgtagtca agtttgaatt gattttgccg tttcggcgcg gaagtaagac ggcagccggg
    10921 ccccaagtcc caaacgcaaa ctgcccaaaa cacaggcatc acgataacaa gaagaagccg
    10981 gcccccattt ttccgaccgc agcgttcgga acgcggcaga caaaccgccg catcgatctt
    11041 tatagccttc tgtatccgac ccttccgcac acggttctcc ggaacgtgca tccctgcgga
    11101 ttttcaactc aaaaactgat taccggtttt atccgaaaac atcggaaaac ccagccttac
    11161 ggaatgccga accggacagg tgcgcccgaa tgccgcccgt ccacgaggtg atcatgaaaa
    11221 gaatgttatt caacgcaacg caggccgaag agctgcgcgt tgccatcgtc gacggacaaa
    11281 acctgctgga tttggatatc gaaacgctgg gcaaagaaca gcgcaaaggc aatatctaca
    11341 aaggcatcat tacccgcatc gagccgtcgc tggaagcgtg tttcgtcgat tacggaaccg
    11401 accgccacgg cttcttgccg tttaaagaag tctcccgctc atacttccaa gactacgaag
    11461 gcggacgcgc gcgcatccaa gacgtgctca aagaaggcat ggaagtcatc gtccaagtcg
    11521 aaaaagacga gcgcggcaac aaaggcgcgg cgctgaccac cttcatcagc ctcgccggac
    11581 gctatctggt attgatgccg aacaacccgc gcggcggcgg cgtatcccgc cgtatcgaag
    11641 gcgaagaacg tcaagaactc aaagccgcta tggcgcaact cgacattccg aacggcatga
    11701 gcatcatcgc ccgtaccgcc ggcatcggcc gcagcgcgga agagttggaa tgggatttga
    11761 actatctcaa acaactctgg caggcgattg aagaagcagg aaaagcgcat cacgaccctt
    11821 acctgctctt tatggaaagc tcgctgctca tccgcgccat ccgcgactat ttccgccccg
    11881 acatcggcga aattctggtg gacaatcaag aagtttacga ccaagttgcc gagttcatga
    11941 gctacgtcat gccgggcaat ataggccgtc tgaaactcta cgaagaccac acgccgctgt
    12001 tttcccgctt ccaaatcgaa caccaaatcg aaagcgcgtt ttcgcgcagc gtcagcctgc
    12061 cctccggcgg cgcgatcgtc atcgaccata ccgaagccct cgtctccatc gacgtgaact
    12121 ccgcacgcgc cactcgcggc gcagacattg aagacaccgc gttcaaaacc aatatggaag
    12181 ccgccgaaga agtcgcccga caaatgcgcc tgcgcgactt gggcggcttg gtcgtcatcg
    12241 acttcatcga catggaaaac cccaaacacc agcgcgatgt ggaaaacgtc ctgcgcgacg
    12301 cgctcaaaaa agaccgcgcc cgcgtacaga tgggcaaact ctcccgtttc ggacttttag
    12361 aattgagccg ccaacgtttg aaaccggctt tgggcgaaag cagccacgtc gcctgtccgc
    12421 gctgcgccgg caccggcgtg attcggggca tcgaatccac cgccctgcac gttttacgca
    12481 tcattcaaga agaagcgatg aaggacaaca ccggagaagt gcgcgcacaa gtgcccgtcg
    12541 atgtcgccac cttcctgctg aacgaaaaac gcgccgagct gtttgcgatg gaagagcgtt
    12601 tggatgtcaa cgtcgtcctg attccgaaca tccacctcga aaatccgcac tacgaaatca
    12661 accgcatccg caccgacgac gtagaagaag acggcgaacc gagctacaaa cgcgtcgccg
    12721 agcctgaaga agacgaatcc gccaaaccgt tcggcggcga aaaagccaaa gccgcccgtc
    12781 ccgaacccgc cgtcaaaggc gtgcgccaca ccagccccgc cccgactgcc gcccccgaga
    12841 aaaaaacctc ctggtgggac agcttcaaag cttggctcaa acgcattttc ggcggcagcg
    12901 aaacccaagc cgcgcccgct gccgaaacct ccgaaaaacg cagcacggca aaccgcagcg
    12961 gcagccgcgc caacaaccgc cgccaaaacc cgcgccgcag caaacgcgaa ggcagcaaag
    13021 tagaagtccg cgaagtggca ggcaaaactg ccggacagga agcgcgtgcc gacaaagccg
    13081 aaacgcgcaa caacggcaac cgccgccgca acgaacgcgg cgaccgtgcc gccggacgcg
    13141 ccgacgaagc ggaaatccaa gaccgcaaca tacagcctgc cgcaaccgtt gcagatgccg
    13201 caccgtccga aaccgaagtg caaaccggaa aacgccgccg caacggcagc cgcagcgaac
    13261 gcggccaaac cgcgccggaa accgccaccg ttgccgaaac aaccgttcag acagcggaaa
    13321 acacgccgtc cgaaccgcat accgcagaag acaaaggcag caagcccaaa tccgaacgca
    13381 accgccgcga acgcgacagc cgagatgcca aagaacgccg cgagcgcaac aatcagcgcg
    13441 accgccgtca aaacggcaaa aaacgcaata ttccgtctgc cgccaaaatc gagcagtacc
    13501 tgaatattca cgacaccgcc gacaaagtcc gttccgccgc cgcgcacgtt ttcggcgaaa
    13561 ccgacgcaaa cgcgccgatt actgtcagca ttgccgatcc ggttgcagaa agggatcttc
    13621 cgacagcatc tcccgccgtt tcaaacggcg acgcaccggt ttatgatgcg gcggaaaaaa
    13681 tccgccgtgc caccgccgcc atcctgcccg aaggcgcgac accgaaagcc gaagcacagg
    13741 aaatgccgtc tgaaaccgca acctttacgg ctgcggcgga acaggcacgg gaaaccgcac
    13801 aaaccggcgg actcgtcctg atcgaaaccg accctgccgc attgaaggca tgggcggcac
    13861 aacccgaagt ccaagccgga cgcggtttgc gccgttccga acagcccaag ccgtctgaag
    13921 tcgcaaccgt ccctgccgaa gaaatgatcc aagtcgaaac ccggcaaggc tgaaccgacg
    13981 gcggcaaaaa gaggttctgt tccgcagaac ctctttttta catgggttcg gatacctgca
    14041 atgccgtctg aaacttcgcc attcccgtga ttaccgaaac attccgtcat tcccatgatt
    14101 cccgcaacat tccgtcattc ccatgattcc cgcaacattc cgtcattccc gtgaaaacgg
    14161 gaatctagaa cctcaaactt tcggataatc tttgaatatt gccgtcgccc aaaggcctgg
    14221 attcccgcct gcgcgggaat gacggcggag ggtggacgat gccgtctgaa acttcgccat
    14281 tcccatgatt accgcaacct ttcgtcattc ccgccacctt tcgtcattcc cgtgaaaacg
    14341 ggaatctaga acctcaaact ttcggataat ctttgaatat tgccgctgcc cgaaggtctg
    14401 gattcccaca acctttcgtc attcccgtga aaacgggaat ctagaacctc aaactttcag
    14461 ataacctttg aatattgccg ctgcccgaag gtctggattc ccacaacatt tcgtcatttc
    14521 cgtgaaaacg ggaatctaga acctctaaac tttcagataa tctttgaata ttgccgtcgc
    14581 ccaaaggcct ggattcccgc ctgcgcggga atgacggttt agaagttgcc cgaaacctca
    14641 aaaaaaaccg aaaccgaaca agccggattc ccgcaacatt ccgtcattcc cgtgaaaacg
    14701 ggaat
//
LOCUS       DAWHRA010000040        14432 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWHRA010000040 DAWHRA010000000
VERSION     DAWHRA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.040"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..217
                     /locus_tag="WIB30_000902"
     CDS             <1..217
                     /locus_tag="WIB30_000902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3796991.1"
                     /translation="SLHIENAAQANLQFARLVLALSHGERGLGCRLPLRFRQILTSLI
                     LRFSFAEAALSDDLLEHHRYTHLRNAV"
     gene            complement(228..551)
                     /locus_tag="WIB30_000903"
     CDS             complement(228..551)
                     /locus_tag="WIB30_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ3796992.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(631..1287)
                     /locus_tag="WIB30_000904"
     CDS             complement(631..1287)
                     /locus_tag="WIB30_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ3796993.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            complement(1390..1917)
                     /locus_tag="WIB30_000905"
     CDS             complement(1390..1917)
                     /locus_tag="WIB30_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ3796994.1"
                     /translation="MYATIGDVMHALHFSASDKAALYREVLPQIESVVADEADWVANL
                     ANTAAVLKEAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGET
                     VVVKDVNAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGEL
                     AKILEKRFEASRQAV"
     gene            2072..2845
                     /locus_tag="WIB30_000906"
     CDS             2072..2845
                     /locus_tag="WIB30_000906"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ3796995.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRIHPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPNNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2852..3202
                     /gene="secG"
                     /locus_tag="WIB30_000907"
     CDS             2852..3202
                     /gene="secG"
                     /locus_tag="WIB30_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ3796996.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3216..3301
                     /locus_tag="WIB30_000908"
     tRNA            3216..3301
                     /locus_tag="WIB30_000908"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3250..3252,aa:Leu,seq:gag)
     gene            4212..4580
                     /locus_tag="WIB30_000909"
     CDS             4212..4580
                     /locus_tag="WIB30_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ3796997.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILLKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4577..4888
                     /locus_tag="WIB30_000910"
     CDS             4577..4888
                     /locus_tag="WIB30_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ3796998.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5381..6309)
                     /locus_tag="WIB30_000911"
                     /pseudo
     CDS             complement(5381..6309)
                     /locus_tag="WIB30_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
     gene            complement(6332..6463)
                     /locus_tag="WIB30_000912"
     CDS             complement(6332..6463)
                     /locus_tag="WIB30_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ3796999.1"
                     /translation="MRNFDKLAEEAAEHAAEGAVKFIDGKPAKKIIAVPGRLANIVV"
     gene            complement(6593..7384)
                     /locus_tag="WIB30_000913"
     CDS             complement(6593..7384)
                     /locus_tag="WIB30_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ3797000.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMKR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNIVPDGAYHTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYGFEDKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLPPTSKAF"
     gene            complement(7614..10346)
                     /locus_tag="WIB30_000914"
     CDS             complement(7614..10346)
                     /locus_tag="WIB30_000914"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ3797001.1"
                     /translation="MQEHYRHAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVLQRVTIGLSPEQEEAMGFGGGSGC
                     GAGYGDGSGSDSRDLPFMPNKWQAWYGVKDDFMVLINSGELDGMNYQTAFDAVAAKLQ
                     AIGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCEKCGDVPVPADQLPVVLPENVVPDG
                     MGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFMESSWYFFRYMSPKFSDGMVSAEAA
                     KYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEGLVNVGEPFERLLTQGMVVCETYYR
                     ENDKGGKDWINPADVELTFDDKGRPVSAVLKADGLPVVISGTEKMSKSKNNGVDPQEL
                     INAYGADTARLFMMFAAPPEQSLEWSDSGVEGAHRFLRRLWRTVYEYLKQGEAVKAFA
                     GSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQFNTAIAAVMELLNQYDKTDTGGEQG
                     RAVAQEVLETAVRLLWPIVPHICETLWSELNGAKLWEAGWPTVDEAALVKSEIEVMVQ
                     VNGKLRGKITVAADASKADLEAAALATEGAVKFMEGKPAKKIIVVPGRLVNIVV"
     gene            10613..11128
                     /locus_tag="WIB30_000915"
     CDS             10613..11128
                     /locus_tag="WIB30_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ3797002.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNIKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GTDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            11135..11578
                     /locus_tag="WIB30_000916"
     CDS             11135..11578
                     /locus_tag="WIB30_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ3797003.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVSAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASSVVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11745..13802
                     /gene="ppk1"
                     /locus_tag="WIB30_000917"
     CDS             11745..13802
                     /gene="ppk1"
                     /locus_tag="WIB30_000917"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ3797004.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAREGIHFYRRRNWTGAQKKWIESYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            14035..>14432
                     /locus_tag="WIB30_000918"
     CDS             14035..>14432
                     /locus_tag="WIB30_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3797005.1"
                     /translation="MKITHCKLKKKVQKELIRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGSVEPDKSDFGGRRKGRRGRGAAGKVVVFGILKHNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTD"
BASE COUNT         3611 a         4022 c         3531 g         3268 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaaccttcag tttgcccgcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg atactgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccaa atacatcgcc gtatgcaggc tgcggatacc gtgatggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgctttca attccgcggg cccaagttgc
      541 acaatatcca tcgcaccccc caaaaaaaac caagcaaaat gccgtctgaa gccccaaacc
      601 cgctttcaga cggcatgacc tgtcaacatc ttaaaaatcg aaaccgccaa acggatcggc
      661 atccttatca tccaaatgcg ccaccaaggt atcgaacagc accttctctt caaacacatc
      721 gcccctgcgc gtaatcaaaa gcgcgcgttg aacaggcttg cgacctacga taaccaccat
      781 gcgtccgcca tctttcaact gttctttcaa cacttcaggc acaaggttta ccgcaccgcc
      841 gacataaacc gcatcaaacg gcgcacctgc ggaaagttcg gtcaacccgt tgttttgcac
      901 ataatcgata ttgtccaaac ccaagccgtc caacaccgct ttggcgcggt tttgctgttc
      961 ggcatcgatg tcgtccgaca ccacacgacc agccaatttt gccaacagcg cggtcgcata
     1021 gcccgaaccc gtgccgattt ccaaaaccgt atcgtttttc gtcagcttca agccctgcgc
     1081 cagccgcgcc acgactttcg gctcgagcat cttatgaccg ttggcaagcg gcagcgccat
     1141 atccgcatac gccaaaccct gcaagtcctc atcgacaaaa agctcgcgcg gaatctccgc
     1201 caaagcgtcc aacacatcaa aatccaatac atcccacgga cggatttgct gttcgaccat
     1261 attgaaccgc gctttttcaa aatccattta gtgctccgtc aaataattgt tccaacaatg
     1321 gcaggcatta taaaaccaca ctcccgccgc gccaacttcg gcacgcgccg acagcccgtt
     1381 ttgtcagtct caaaccgcct gacgcgaagc ctcaaaccgc ttctccaaaa tcttcgccag
     1441 ttcgcccaaa tacaaagcat ccgtctcatc aaactgcgcc aaatgttcgc tgtccgcgtc
     1501 caacacgccg atacagcggc cgtctgaaaa cagcggcacg acaatctccg aacgtgacaa
     1561 agacgaacag gcaatatggt cgggatgcgc gttgacatcc ttaacaacca ccgtttcacc
     1621 cttcgcccaa gcctgaccgc acaccccgcg accgaacgga atccgcgtac acgccaaagg
     1681 cccctgaaac ggtgccaaaa ccaattcgtc cgaacgcgta tcgaccaaat aaaaacccac
     1741 ccaaaaccaa ccgaacgcct ccttcaaaac cgccgccgtg ttcgccaaat tcgccaccca
     1801 atccgcctcg tcagccacca cagactcaat ctgcggcaac acctcccgat aaagcgcggc
     1861 cttgtccgaa gccgaaaaat gaagcgcgtg catcacatct cctatagttg catacatatc
     1921 aggcggccat tataaaacag cctgcccgaa acaacattcc aaaccgcccg gccggccgct
     1981 tcaagttgcg aacccgccgc atatccacta aacttcacgt tgcaccgcgc cacacgcggc
     2041 agacaaaaaa acacgacacg gagcaaaaaa gatgtatcgc caaatcggaa tgtgggatca
     2101 aaaatgggtc atcggcaact ggaaaatgaa cggccggctc caaaacaaca acgcactgat
     2161 gcaccgcttc cgcatccacc ccaccgccga acgcgtcctc atcggactcg ccgccccgac
     2221 cgtttacctg ctgcaactgc acaacgccat gcaaatcgtt ttaaacaacc gcatcctcac
     2281 ctgcgcccaa gacgtgagcc gcttccccaa taacggcgcg tacaccggcg aagtgtccgc
     2341 cgaaatgctc gccgacaccg gcacagacat cgtcctcatc ggacactccg aacgcagcct
     2401 ttatttcggc gaaaaaaacg aaatccaacg ccgcaaaatg gaaaacgtcc tcaacgtcgg
     2461 actcatcccg ttattgtgcg tcggcgaaag cctcgaagag cgcgaagccg gcaaagaaca
     2521 cgaagtcatc gcccatcagc tttccatcct gcaagggctg gataccaaaa acatcgccgt
     2581 cgcctacgaa cccgtctggg cgatcggcac cggcaaagtc gccaccgtcg aacagattgc
     2641 cgatatgcac gcattcatct acaaagaaat cttgtctttg tgcggaagcg atgttaaaat
     2701 ccgcgtcctt tacggcggaa gtgtgaaagc ggacaacgcg gccgacatct tcgcagtacc
     2761 ttatgtggac ggcgcactcg tcggcggcgc gtcattgtcg tacgactcct ttaccgccat
     2821 catcagtgcc gcacaaaatg cgtagaaaaa tatggaagcc ttcaaaaccc taatttggat
     2881 tgttaatata atttccgctt tggccgtcat cgtgttagta ttgctccaac acggcaaagg
     2941 cgcggatgcc ggcgcgactt tcggatcggg aagcggcagc gcgcaaggcg tattcggctc
     3001 tgccggcaac gctaacttcc tcagccgctc gaccgccgtt gcagcaacat ttttctttgc
     3061 aacctgcatg gctatggtgt atattcacac ccacacgaca aaacacggtt tggacttcag
     3121 caacgtacaa caaactcagc aagcacccaa acccgtaagc aataccgaac cttctgcccc
     3181 tgttcctcag cagcagaaat aacagttttt caaatgccga catggtgaaa ttggtagaca
     3241 cgctatcttg agggggtagt ggccgtaggc tgtgcgagtt caaatctcgc tgtcggcacc
     3301 aacacacaaa aacgcctgaa aatttcaggc gttttttgtt caatcccatc caacgctgtc
     3361 aaccaattcc aatcaataca aggatttcag gctgattgtt atcctgccgt cccccttcct
     3421 gacagtgcaa tcccgtccaa tccgccctaa ttgaagtaac ctaaaattta cggtatcttt
     3481 tgcggtatct gaaaaatacc tcgaaaaaat accgcaaaaa taaagctgaa cgaccgccaa
     3541 atcaggaatg ccaagcggaa aagagcttgc ggggaatact gccaagacgt agggaacaag
     3601 ggggaaaccg tccaagatgc agggcggttt tttttgggtt tttggaaaaa acctatacta
     3661 ggaagcgata cccttagttg ttaccttgtt accggggaaa agttagataa ataagcatat
     3721 gaaatatagt gaattaaatt taaatcagga caaggcggcg agccgcagac agtacaaata
     3781 gtacggcaag gcgagccaac gctgtaccgg tttaaattta attcactata aaataagaaa
     3841 aagataaaaa attggtaaca aatgcggtaa caaatggtaa cgaatcggta acaacttttg
     3901 gggttttccg gtttttcacc gtcttggcag tgggagcgta gcggaatgaa aagccaaaac
     3961 gcacggaacc gcgcctattt tgaggcagga atggtggtta aaccgcttgg ttatatacgg
     4021 gcataggaag acagcgaaac gcgctaaaaa cgcaatttga gccattaaaa gcgattgatt
     4081 aaaaaataat caggttagct gccggtgttt caggcggcat aaacggagaa attgcggggc
     4141 ataaaaaagg cagcttgccg tgttgtctgt ctctggtata attccaagta tcactaatca
     4201 acggctacac aatgcggata ttcaaaaacc aatggatagt gaaatttgcc aagaagcaca
     4261 aaatcaacga ttccgagctg ctggaagcgg tagagcgggc ggataacggg ctgatagacg
     4321 cagatttagg cggcggtgtg attaagcagc gcatagcaag gcaaggacaa ggcagaagcg
     4381 gcggctatcg cagcctgata ctgctcaaac aggcagacag ggcatttttt gtttacgcct
     4441 ttgccaagaa cgacagggaa aacatttcgg ataaagaact ggacgtttac cgaaaagccg
     4501 ccgcatatta tctgaaatac acgcgggcag agctggcggc tttgaaagaa gacggcatta
     4561 tcacggagat agaatcatga aatacaaaag cgaggcatta gccgccattc atgaaatgat
     4621 ggaaggggct tacaacatcg gcgcaatcga caaaaagact atgcgcggct ttgacaagtc
     4681 atgcctgacc gaaatcaaac cgttgagcgg cggagacatc aaggcaatca gggagaagga
     4741 ggcactatcg caagccgctt tcgccatcta tctcaacgtg ggaaaaaatc acgtttcggc
     4801 ttgggagcgg ggcgttaaaa agcccagcgg cgcggcgttg aagctgctga ccatcgtcaa
     4861 aaacaagggt atcgaagcca ttgcgtagcc gacttggcaa acggcaaaat cagcaagttc
     4921 acaatagacg cgctgctgaa tatgcctgcc aaaacaggca agaccgccga actgaatatc
     4981 agggcgtagc cgcacaaatg ccccgaccgc atcaaaccaa gccgaaacgg cggcggtgca
     5041 gacgacatag cccgacagca aggcacggcg cagacgggcg cgaaacccga aacatccccg
     5101 accgcgaggc acggggattt ttttgcgccc gttgcagggg ggattggatt taagcggcgc
     5161 gggcttgaag gcaaaacggg cggggcgcag aactgtttaa atgccgtctg aatctcaaac
     5221 gatttcagac ggcattttga aacaacggct caaattctcg atccccttcc cttaacgccg
     5281 acgtttttta tagtggatta acaaaaacca gtacagcgtt gcctcgcctt gccgtactat
     5341 ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atattaacgc
     5401 gccccttatt tctgacactt tgctcataaa caggcataac ggtcggcaac aaccgtttta
     5461 ggttttctat acgggcattg tttgtcggat gagtagaggt aatagcataa ataaagccgt
     5521 tttggtcgtt ttcctgattc attttttccc aaaccctgac agcggccgcc ggatgatagc
     5581 ctgcctgcgc catcaacatc attcccccct catcggcttc ttcttccaag ctgcggctat
     5641 aaggcaaggt aagaccgtac gtccccaaaa tatccatacc caatccgacc aattccggat
     5701 tagtgtccgg ttttttgtct aatataatct gcgtgcctat ctgcgccgcc gtattggtca
     5761 aaatttgttg cccgacctta tttttaccgt gttcatgcag ggcgtgcgtc atttcatgcc
     5821 ccataatggc ggcaatttcg tcatcggtca gcttgagttt gtcgactatc cccgtataaa
     5881 acgccatttt tccaccgggc atcgcccacg cgttcagctc atcgtttttg aaaaccgtca
     5941 ttttccagtc aaacctatgg ccggtattat ttgccgcatc ggcataaggc agcatacgtc
     6001 gaaatactgc ctgcaccctg cgggctgttc tggatgtggt atcgacattg ccggcagact
     6061 tgttcaactc aaccgttttc atataatctt tggcagccgc agcgttcatt gtggcggaat
     6121 cctgaccgta aacatcagca acgaccgtac aagcccccaa taccgagatt actgccgaca
     6181 ggcagagtat ccgtttaaag gaaggaagga aggaaggaag gaaatttcat attaggctta
     6241 ccccttaaaa aattaaattt aaaaaatgcc gtctgaatct aaaacggatt tcggacggca
     6301 tcttaacatt gtttgatgtt tttaaaaaga tttacaccac gatgttcgcc agtctgcccg
     6361 gtacggcgat gattttcttg gcaggcttgc cgtctatgaa tttcaccgcg ccttcagcgg
     6421 cgtgttcggc agcttcttca gccaatttgt cgaaattacg cataaattgc caataattcc
     6481 ccgacttttt tacggctgct gccttttgcg gcaatattgc gctgtacttc aactgttttc
     6541 agaatagcag aagaataaat atcccttgtg aattcagtat catgatttga aattaaaacg
     6601 ccttggaagt cgggggcaat ttgtcgattt ttttgtaaag tccgcgacca atgaattcga
     6661 tcgtattttg gtcgcgcaaa atttgcaact gttggcggat tttgtctctg atatggttgt
     6721 tttggggaaa ttggatggat agtttgtctt caaatccata catttgcgac aatgtgaatt
     6781 cttcggggag ttggtcgata catttcataa cagccaaaag ccagccttta cgctccgcat
     6841 tttggttgcg taaaaacagg ttggattgcc attttttcag aacggtttcg ggttcgataa
     6901 tgcgggaatc gtctattaag aatatttttc cgctttcagg caaaggggcg agattgatgg
     6961 aacacataat gtggtgcggc cggtttttaa tgcctttatt tctgggaata atcatatccg
     7021 gcgtgatgaa atgtttgggt acaagcacca attgccgtat ggagtaatcc gcttttttat
     7081 atgcaagaaa gaaaaagttg gggttggtat ctgaccggat gcgctccaac atggtgtgat
     7141 atgcaccgtc aggcacgatg ttgcctatgg ttttttgatt tttactcttt aattcatatt
     7201 gctcgtggca atttgggcaa aagaggtctg caacaggttt gttattggca aatctcttca
     7261 tcggcttgct tccgcaacag ggacagtagc cgtttttttc cagccaagcc tcgctcatta
     7321 cacggatttt gtgggttgct ttattttgtt gctttcccaa ttcagtatcg aaaaataaat
     7381 tcatgttttg gattttgaga ttaagtttta gtggattaac aaaaatcagg acaaggagac
     7441 gaagccgcag acagcagggc aaggcggggc aacgccgtac tggtttaaag ttaatccgct
     7501 atatttgagg tctgtaatgc agacaaccca atctgcctta aaaaggccgt ctgaaaccct
     7561 gttttccagt tttcagacgg cctttatccg tatggctaaa ccttaagagc ggtttagacg
     7621 acgatgttca ccagtctgcc cggtacgacg atgattttct tcgcgggctt gccttccatg
     7681 aatttcaccg cgccttcggt ggcgagtgcg gcggcttcga ggtcggcttt ggaggcgtcg
     7741 gcggcgacgg tgattttgcc gcgtagtttg ccgttgactt gaaccatcac ttcgatttcg
     7801 gatttgacca aagcggcttc atcaactgtc ggccagcctg cttcccacag tttcgcgccg
     7861 ttcaattcgc tccacagggt ttcgcagatg tgcggcacga tgggccacag caggcgtacg
     7921 gcggtttcca atacttcttg ggcgacggcg cggccttgtt cgccgccggt gtcggttttg
     7981 tcgtattggt tgagcaattc catcacggcg gcgatggcgg tgttgaactg ctggcggcgg
     8041 ccgtagtcgt cgctgacttt ggcagtggtc gcgtgcagtt tgtggcgcag gtctttgagt
     8101 tctttagaca aaccgtcttg gctgcctgcg aacgctttga ctgcttcgcc ttgtttcagg
     8161 tattcgtaaa cggtacgcca cagacgacgc aggaagcggt gtgcgccttc gacgccgctg
     8221 tcgctccatt cgagggactg ttcgggcggt gcggcgaaca tcatgaacag gcgggcggtg
     8281 tccgcgccgt aggcgttaat cagctcttgc ggatcgacgc cgttgttttt ggatttggac
     8341 attttttccg tgccgctgat gacgacgggc agaccgtcgg ctttgaggac ggcggaaaca
     8401 gggcggcctt tgtcgtcgaa cgtcagctcg acatcggcgg ggttgatcca atctttgccg
     8461 cctttgtcgt tttcgcggta gtaggtttcg caaacgacca tgccttgcgt cagcaggcgt
     8521 tcaaacggtt cgccaacatt gaccaagcct tcgtcgcgca tcagtttggt gaagaaacgc
     8581 gcgtacagga ggtgcaaaat ggcgtgttcg ataccgccga tgtattggtc gaccgcgccc
     8641 cagtatttcg cggcttctgc cgataccatg ccgtctgaaa acttgggcga catatagcgg
     8701 aagaagtacc agcttgattc catgaaggtg tccatggtgt cggtttcgcg tttcgccgcg
     8761 ccgccgcagc atgggcaggt ggtttcgtaa aactcgggca tttttgccaa aggcgaaccc
     8821 atgccgtcgg gtacgacgtt ttcaggcaaa acgaccggca gttggtcggc ggggacgggt
     8881 acgtcgccgc atttttcgca atggacgatg ggaatcgggc agccccagta gcgttggcgc
     8941 gaaatgcccc agtcgcgcag gcggtattgg gttttcggtt cgccagctcc gatagcttgt
     9001 aatttagcgg cgacagcatc aaatgctgtc tgataattca tgccgtccag ttcacctgag
     9061 ttgattaaga ccatgaagtc atctttgact ccataccatg cttgccattt attaggcata
     9121 aacggcaaat ctcttgaatc ggaaccacta ccatcaccat aaccagcacc acatccggaa
     9181 ccgccaccaa aacccatagc ttcttcttgc tctggcgaaa gtccgattgt tacgcgttgt
     9241 aagacctgtt ttttcggcag gctgtatttg gcggcgaact caaaatcgcg ttcgtcgtgc
     9301 gccggaaccg ccatcactgc gccgtcgccg tagccccaca atacatagtt ggcaatccac
     9361 acttccagct tgtcgccgtt gagcgggttg acgacgtagc gaccggtcgg cacgcctttt
     9421 ttctccatcg tcgccatatc ggcttcggca accgaaccgg ctttgcattc ggcaataaat
     9481 gcctgcaatt cgggtttgtc ggcggctgcg gcggctgcca gcggatgctc ggcggcaacg
     9541 gcaacataag tcgcacccat cagcgtgtcg gggcgggtgg tataaacttg caggaatttc
     9601 gcgtaatcgc cttccaagcc ttgtttgctg tcgtctgaaa cggcgaagcg cacggtcata
     9661 ccgcgcgatt tgccgatcca gttgcgctgc atggttttga cttgttccgg ccagtgttcc
     9721 agcttgtcca agtcgttgag cagctcttcg gcgtaatccg tgattttgaa gtaatacatc
     9781 gggatttcgc gtttttcgat caatgcgccg gaacgccagc cgcgtccgtc gatgacttgc
     9841 tcgttggcaa ggacggtttg gtcgacaggg tcccagttca ccgtgccgtt tttgcgatac
     9901 acgatgcctt tttcaaacag cttggtaaac agccattgtt cccagcggta gtattcgggt
     9961 ttgcaggttg cggtttcgcg cgcccagtca atcgcaaaac ctaggctttt gagctgggtt
    10021 ttcatgtatt cgatgttatc gtacgtccaa gcggcagggg cgacgttgtt tttcatcgcc
    10081 gcgttttccg ccggcatgcc gaacgcgtcc caacccatag gctgcatgac gttgaagccg
    10141 tttaaaagtt tgaagcggct caatacatcg ccgatggtgt agttgcgcac atgccccatg
    10201 tgcagcttgc cgctgggata ggggaacatg gagaggcaat aatatttggg tttggaagcg
    10261 tcttcggaga cgttgaaaat acgggcgtcg tcccattttt tctgcgccgc aggctcgatg
    10321 gcggcgtgcc ggtaatgttc ttgcatggtc attctgtttt tcacttgaaa acgttaggaa
    10381 tttaaaggag gaattataac aggttgccga tgtcggcgaa tcggcaaatt gccggcagga
    10441 tgcgtaaatt cgcacgcgca tcattccgta tgccgtacaa atacaccgcg tttactgata
    10501 cgcacgtttt ttatgctaat attacaaacc aaaatcaaac gtttaaaacc ctcccgatcc
    10561 agctcttccg aacaaaaggc agacgggcat cgggtaaaaa aggattctac atatgaaaat
    10621 caaacaaatc gtcaaaccgg gcttggcagt attggcggcg ggcgttctgt ctgcctgtgc
    10681 aaccaaaagc aacatcaaag ccgacggcac gaccgacaat ccggttttcc cgaaacccta
    10741 ttccgtaacg ctcgacaaca agcgcggcac attcccgact tatgacgaac tggatcagat
    10801 gcgccccggc ctgaccaaag acgacatcta caaaatcctg ggtcgtccgc attacgacga
    10861 aggtatgtac ggcgtgcgcg aatgggatta cctgtttcac ttccacaccc ccggcgtagg
    10921 taccgaccct gaaaacactt ccggcgtgga agatgttact acctgccaat acaaagtgat
    10981 tttcgataaa gacaaatttg cccgcagctt ctactggaac cccgtcttcc cgaaagatgc
    11041 cgcctgtccg ccgcccgcac ccaaagccga gccgcaagtc atcatccgcg aaatcgtgcc
    11101 ggcaaaaccg aaacgtatcc gccaataatc cgacatgccg ttccgcctgt ttttagggat
    11161 attatgcggc ctatcgatgg ttgcccccgt atatgcacag gggcagccgg atgcggtcag
    11221 tgcctatatt caaaagaaaa aagtcatcgt aaacacatcc aaagcggaac tctgttttgc
    11281 cgatgacagg cagtgccacc ccgtcctcat cggtgttgcc acgcccaagg ggacgttcgg
    11341 gctgacgctg aacagtaccg acaagcccgg atacggcggc gaagtcatcg gtttcaagca
    11401 ggagggcgat tttcttttcg ccctgcaccg cgtttggaat cagataccgt cggaaaggcg
    11461 gaacgaacgt atcgcatctt cggttgtgtc cgacaggatt atgaccaacg gctgcatcaa
    11521 cgtcagcgac gcggtatacg aaaaactgcg ccattatttt gtattggaag taatttaaac
    11581 agacggatac cgtatatagc ggtatccgtt ttcacatcat gccccaaatc ctgataaaag
    11641 ccatgcatat gccgtctgaa aaacgttaaa aacaccttca agatttcagg gggcattacc
    11701 tgccttttcc aacagaacaa tcagacagca cctaggagaa ccttatgccc gaacaaaacc
    11761 gcatcctctg ccgcgaactg agcctgctgg cattcaaccg ccgagtgttg gcacaggcgg
    11821 aagacaaaaa cgtccccctt ttggaacgcc tgcgcttcct gtgcatcgtg tcgtccaatc
    11881 ttgacgagtt tttcgaagtc cgcatggcgt ggctcaaacg tgaaaacaag ctgcaccccc
    11941 ggcgcaggct ggacaacggc aaaatgccgt ctgaaaccat cgccgacgtt accgaagcgg
    12001 cgcgctccct gatacggcac cagtacgacc tgttcaacaa cgtccttcag cccgagttgg
    12061 cgcgggaagg catccatttt taccgccgcc gcaactggac gggcgcgcag aaaaagtgga
    12121 tcgaaagcta tttcgaccgc gaattgctgc cgattctgac ccccatcgga ctcgaccctt
    12181 cccacccctt cccgcgcccg ctgaacaaat cgctcaactt cgccgtcgaa ctcgacggca
    12241 cagacgcatt cggcaggcct tcgggtatgg cgattgtcca agcaccgcgc atcctgccgc
    12301 gcgtcgttcc cctgccgtcc gaactgtgtg gcggcggaca cggcttcgtc ttcctgtcgt
    12361 ccatcctcca cgcccacgtc ggcaaactct tcccaggcat gaacgtcaaa ggctgccacc
    12421 agttccgcct gacgcgcgac agcgacttga ccgttgacga agaagacctg caaaacctcc
    12481 gcgccgccat ccaaaacgaa ctgcacgacc gcgaatacgg cgacggcgtg cggctcgaag
    12541 tcgccgacac ctgtcccgcc tacatccgcg actttctgct cgcgcaattc aaactgaccg
    12601 ccgccgaact ctatcaggtc aaaggcccgg tcaacctcgt gcgcctcaac gccgtccccg
    12661 acctagtcaa ccgccccgat ttgaaatttc ccacacacac gccgggcaga ctgaaagcct
    12721 tgggcaaaac cgcgtccata ttcgatttgg tgcgccaatc gcccatcctg ctgcaccacc
    12781 cctaccaatc gttcgacccc gttgtcgaaa tgatgcgcga agctgccgcc gaccccgacg
    12841 tgcttgccgt caaaatgacg atttaccgca ccggcacgcg ttccgaactc gtccgcgccc
    12901 tgatgaaggc ggcactcgcc ggcaaacaag taaccgtcgt cgtcgaactg atggcgcgtt
    12961 ttgacgaagc caacaacgtc aactgggcga agcagctcga agaggcgggc gcgcacgtcg
    13021 tgtacggcgt gttcggctac aaagtccacg ccaagatggc actggtcatc cgccgcgaag
    13081 acggcgtact caagcgttac gcccacctcg gcacgggtaa ctaccaccaa ggcacatcgc
    13141 gcatctacac cgacttcggc atcattaccg ccgacgaaca aatcaccgcc gatgtgaaca
    13201 cattgtttat ggaaatcaca ggcttgggca aacccgggcg gctgaacaaa ctctaccaaa
    13261 gcccgtttac cctgcacaaa atggttatcg accgcatcgc acgcgaaacc gaacacgcaa
    13321 aagccggcaa accggcgcgg attaccgcca agatgaattc gctcatcgaa ccgaccgtca
    13381 tcgaagccct gtatcgggca agcgcggcag gcgtacaaat cgatttgatt gtacgcggta
    13441 tgtgcacctt gcgcccgggt gtaaaaggct tgtccgaaaa catccgcgtc cgctccatca
    13501 tcggcaggca gctcgaacac gcgcgcgtgt attgcttcca caacaacggc gcggacgata
    13561 cctttatctc cagcgcggat tggatggggc gcaacttctt ccgccgcatc gaaaccgccg
    13621 cgccgattac cgcgcccgaa ctcaaaaaac gcgtcatccg cgaaggtttg gaaatggcat
    13681 tggcagacaa tacgcacgcg tggctgatgc agcccgacgg cggctatatc cgcgccgcgc
    13741 ccgccgaggg cgaatccgaa gccgacctgc aaaacgattt gtggacactg ctcggaggct
    13801 gacccgcacc gccccaatca aaaaccatgc cgtctgaaac ctttccgttt cagacggcat
    13861 ggttttacag caatctaaac agggcggacc ggagtcaaaa acacaccttc gccattcctg
    13921 cacaagcact tcccctatac gctcccaacc ccaagccgcc gcattccaga cggcattata
    13981 gtggattaaa ttttaggggc tgtactagat tagcagatat gttaccctgg gaatatgaag
    14041 ataacacact gcaaattaaa gaaaaaagta cagaaagaac tgatccgttt ttttgtactc
    14101 gaagttaccg cccgttctgc cgccgatatt ttgggtatcc atcccaattc ggcagcactg
    14161 ttctaccgta aaatccgcac ggttatcaac catcatttgg ccttggctgc cgatgaggtt
    14221 tttgagggct ctgtcgagcc ggacaaaagc gatttcggcg gacggcgtaa aggcagacgt
    14281 ggtcgcggtg cggcaggaaa agtggttgtc ttcggcattc tgaaacacaa cggacgggtc
    14341 tataccgttg tggtggataa tgccaagtct gaaacgttac tccctgtcat caagaagaaa
    14401 atcatgccgg acagcattgt ttataccgat ag
//
LOCUS       DAWHRA010000041        14344 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWHRA010000041 DAWHRA010000000
VERSION     DAWHRA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.041"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            170..793
                     /gene="lolA"
                     /locus_tag="WIB30_000919"
     CDS             170..793
                     /gene="lolA"
                     /locus_tag="WIB30_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ3797006.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1066..2205
                     /locus_tag="WIB30_000920"
     CDS             1066..2205
                     /locus_tag="WIB30_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3797007.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAHPPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQEIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARDLM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2337..3358
                     /locus_tag="WIB30_000921"
                     /pseudo
     CDS             2337..3358
                     /locus_tag="WIB30_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            3399..3929
                     /locus_tag="WIB30_000922"
     CDS             3399..3929
                     /locus_tag="WIB30_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ3797008.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(3963..4079)
                     /locus_tag="WIB30_000923"
     CDS             complement(3963..4079)
                     /locus_tag="WIB30_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ3797009.1"
                     /translation="MPSEPEKRLSDGIFACNSNADGQNQGASDTVIGCVPSA"
     gene            complement(4088..4275)
                     /locus_tag="WIB30_000924"
                     /pseudo
     CDS             complement(4088..4275)
                     /locus_tag="WIB30_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(4294..5889)
                     /locus_tag="WIB30_000925"
     CDS             complement(4294..5889)
                     /locus_tag="WIB30_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ3797010.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRNTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVKPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(5996..6391)
                     /gene="hisI"
                     /locus_tag="WIB30_000926"
     CDS             complement(5996..6391)
                     /gene="hisI"
                     /locus_tag="WIB30_000926"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ3797011.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGGAWETADAVLKDEKEIYGSTH"
     gene            complement(6422..7189)
                     /gene="hisF"
                     /locus_tag="WIB30_000927"
     CDS             complement(6422..7189)
                     /gene="hisF"
                     /locus_tag="WIB30_000927"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ3797012.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(7202..7939)
                     /gene="hisA"
                     /locus_tag="WIB30_000928"
     CDS             complement(7202..7939)
                     /gene="hisA"
                     /locus_tag="WIB30_000928"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ3797013.1"
                     /translation="MLLIPAIDLKEGRCVRLRQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQTVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(7972..8610)
                     /gene="hisH"
                     /locus_tag="WIB30_000929"
     CDS             complement(7972..8610)
                     /gene="hisH"
                     /locus_tag="WIB30_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ3797014.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVWTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCIAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDRQGCRLKVPHMGWNTVRQTQNHPLFKDIPQNTRFYFVHSYY
                     FDPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(8741..10243)
                     /gene="pta"
                     /locus_tag="WIB30_000930"
     CDS             complement(8741..10243)
                     /gene="pta"
                     /locus_tag="WIB30_000930"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ3797015.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(10428..11486)
                     /gene="fbpC"
                     /locus_tag="WIB30_000931"
     CDS             complement(10428..11486)
                     /gene="fbpC"
                     /locus_tag="WIB30_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ3797016.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(11507..13024)
                     /gene="fbpB"
                     /locus_tag="WIB30_000932"
     CDS             complement(11507..13024)
                     /gene="fbpB"
                     /locus_tag="WIB30_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ3797017.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(13092..14087)
                     /gene="fbpA"
                     /locus_tag="WIB30_000933"
     CDS             complement(13092..14087)
                     /gene="fbpA"
                     /locus_tag="WIB30_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ3797018.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
BASE COUNT         3213 a         3569 c         4209 g         3353 t
ORIGIN      
        1 gcggcacaca ttaaagggca gcgtggcgcg cctgcctttt ccgagcggtc aaaaaaatca
       61 gccctcggaa aacgctgttt gtaaaatgca aaccgcccgt aacgccgccc gtatgattgt
      121 tttgccgcgc cgatacttta cgccacaccc atcccaacaa ggaaaaataa tgatgaaacc
      181 gcacaacctg ttccaatttc tcgccgtttg ctccctgacc gtctccgtcg cttccgcaca
      241 ggcgggcgcg gtggacgcgc tcaagcaatt caacaacgat gccgacggta tcagcggcag
      301 cttcacccaa accgtccaaa gcaaaaagaa aacccaaacc gcgcacggca cgttcaaaat
      361 cctgcgcccg ggcctcttca aatgggaata cacttcgccc tacaaacaga ctattgtcgg
      421 cgacggtcaa accgtttggc tctacgatgt cgatttggca caagtgacca agtcgtccca
      481 agaccaggcc ataggcggca gccccgccgc catcctgtcg aacaaaaccg ccctcgaaag
      541 cagctacacg ctgaaagagg acggttcgtc caacggcatc gattatgtgc tggcaacgcc
      601 caaacgcaac aacgccggct accaatacat ccgcatcggc ttcaaaggcg gcaacctcgc
      661 cgccatgcag cttaaagaca gcttcggcaa ccaaacctcc atcagtttcg gcggtttgaa
      721 taccaatccc caactctcgc gcggcgcgtt caagtttacc ccgcccaaag gcgtggacgt
      781 gttgagcaac tgatgccgtc cgccccgatg ccgtctgaaa gccgccgagg cttcagacgg
      841 cattttcacg caggcggaac aatgtcccgc attaccgccc gatcgggcat cggaacggca
      901 aacccgtgaa aattaacggt tgcgccccgg ctgtttttgc cgtttaatgc aaaccttgct
      961 gcaccaaggg ccaagaaagc cgaccggccg cccccacagc ttccgatgca ggcggcccgg
     1021 ccgtccctgc aatgtttttt atttttgaac gaaaggtcga aaaccatgaa aaaaacactg
     1081 gtggcggcgg caatcctgag cctcgccttg actgcgtgcg gcggcggaag cgataccgcc
     1141 gcccaccccc cctccgccaa gcccgaagcc gaacaatcgg gcaaactcaa tatctacaac
     1201 tggtcggatt atgtcgatcc cgaaaccgtc gccgcctttg aaaaagaaac cggcatcaag
     1261 acgcgttccg attattacga cagcaacgaa acactggagg caaaagtcct gaccggcaaa
     1321 tccggctacg acctgaccgc gccgtccatc gccaacgtcg gccggcaaat caaagcgggc
     1381 gcgtatcagg aaatcgacaa ggcgcaaatc ccccattacg gcaacatcga taaagatttg
     1441 ctgaaaatga tggaagccgt cgatccgggc aacgaatacg ccgtccccta tttctggggc
     1501 atcaacacct tggcaatcaa cacgcggcag gtgcaaaagg cattgggtac ggacaagctg
     1561 cccgaaaacg aatgggattt ggtgttcaac cccgaataca ccgccaaact caaatcctgc
     1621 ggcatcagct atttcgacag cgcaatcgaa cagattccct tggcgttgca ctatttgggc
     1681 aaagacccca acagtgaaaa tcccgaagac atcaaagccg ccgtcgatat gatgaaagcc
     1741 gtccggggcg acgtgaaacg cttcagctct tccggctata tcgacgatat ggcggcaggc
     1801 aacctgtgtg ccgccatcgg ttacggcggc gatttgaaca ttgccaaaac ccgtgccgaa
     1861 gaagccgcaa acggcgtgga aatcaaagta ttgaccccga aaaccggcgt gggcgtgtgg
     1921 gtggattcct ttatgattcc gcgcgacgcg caaaacgttg ccaacgccca ccgctatatc
     1981 gactacacgc tccggcccga ggtggcggcg aaaaacggca gcttcgttac ctacgcgccc
     2041 gccagccgcc ccgcgcgcga tttgatggat gaaaaataca cctccgacgc atcgattttc
     2101 ccgaacaaag aactgatgga aaaaagtttc atcgtatcgc ccaaatccgc agaatccgcc
     2161 aaactgggcg tgaagctgtg gcaagggctc aaggccggaa aataaccgca acctctgccg
     2221 tctgaaacct ttcgggcggc aggaaacggc gcgtccgtta tcaaacaggg ggcgtttccc
     2281 cgcccgcctg ttatgattgg gttaagatta aaatgattta gtaaaatgag aaagatatgg
     2341 atttaagtat cgtagttccc atttataatg tcgagagtta tttggaagcg tgtctaaatt
     2401 ccatagaacc tatattaagt aatgaaaatg tcgaacttat ccttgtgaat gacgggtcaa
     2461 aagacggaag tgaagatata tgttataaat atatagataa aacaacaaac aacaaacaac
     2521 aaacaacaaa caacaaacaa caaacaacac cagataccga acaccaaaca tcaaatatat
     2581 atatcaggat aaccaaggga cggcagaagc taggaatacc ggaataaaaa attcaaacgg
     2641 aaaatatata gtctttattg attcggatga ttttattaat tgtcaggtct tgctggattt
     2701 tcttagtaaa gatgatattg atatgccgga tgtggttttt ttaaatgcgg ttaaatatga
     2761 taagggaagt gtttcacatt ttggcgaaga ttatcagcct gaaaaaatac tcaagcaatc
     2821 caaagtcgaa gttttgaaag gattatgccg atttagaaaa tttccgggtt cggcgtgtaa
     2881 taagattata aaaagggaat tgattatcaa agaaaaactg ttttttgaaa aagaggttta
     2941 tgccgaagat atcgaatggt caatgagatt atttaatgcg gcaacaactt tttcttattt
     3001 ggacggttgt tattactatt atcggcaggg aagaaaagat tctaaaacag gaactgtttc
     3061 ggaaaaaagt ataaagtcat tattatatat tttggagaaa aatgcggaaa tggaatttga
     3121 tagggatata tcaagttatc tttattcttt tctttcctac gaatatctcg ttttgctttt
     3181 tatcatgacg agtaaaaata tagagtgtga tgctgatata aaaagaaggg cgtatcattt
     3241 aaggtttatg ctgttaaagt ccaataaatt gatatataag ctgatattcc cgataatcac
     3301 attattcggg gtcgatatta caggcaggat tttaaaagca atcaggggga atatttaata
     3361 aatcctttaa cactatacat cttaccgaag gaggaaaaat gaacgcaatc cgaactttcc
     3421 aaaaccgcac gcccgaaatc cacgaaagct gtatgataga cgaagcgtgc gtcgtcatcg
     3481 gcgaagtgtc gcttgccgaa gacgtttccg tgtggccgtg cgccgtgttg cgcggcgatg
     3541 tgaacagcat caccgtcggc gcgcgcagca atatacagga cggcagcgtc ctgcacgttt
     3601 cccacaaaac cgccgccaaa cccgaagggt cgccgctggt tatcggcgaa gacgttaccg
     3661 tggggcacaa agtgatgctg cacggctgcc gtatcggcaa ccgcgtcctg gtcggcatgg
     3721 ggacgacggt tctggacgat gccgtgattg aggacgaagt gatgatcggc gcgggcagcc
     3781 tcgtgcctcc gcgcaaacgt ttggagggcg gctatcttta tgtcggttcg ccggtcagac
     3841 aagtgcgcgt gttgaccgat gaggaaaaag cctttttgaa gtattccgcc gcgcattatg
     3901 tgaagctgtc gaaacagtac gggatgtgaa atcacatcgg cgttcttgcg tcagccccaa
     3961 attcatgcgg atgggacgca tccgataacg gtatccgatg cgccttgatt ttgaccgtct
     4021 gcgtttgagt tgcaggcaaa aatgccgtct gaaagccttt tttcgggttc agacggcatt
     4081 ttatcgccta aagtttgacc gaatgttcgc gcgtttcgtg gaacacgatg tccggccagc
     4141 gttcttgcgt cagccctaaa ttcatgagga cgggatgccc gataacggta tccgatgcgt
     4201 cttgattttg atcggtgcat ttgagttgca ggcaaaaatg ccgtctgaaa gccttttttc
     4261 gggttcagac ggcattttat cgccgattgc tttttacagt ttaaccgaat gttcgcgcgt
     4321 ttcgtggaac acgatgtccg gccaacgttc ttgcgtcaac cccaaattca cgcggttggg
     4381 ggcgaggtag gcgaggttgc cgcccgcgtc gatggcgagg ttgcccgcgt tggctttttc
     4441 aaactccgcc agttttttct tgtcgtcgca cgatacccag cgcgccgacc agatggatgc
     4501 gctgtcgaac acggcttcta cgccgtattc gttggccagc cgcgaggtaa cgacttcaaa
     4561 ctgcaacacg ccgaccgcgc ccaaaatcaa atccgcgccg ctcatcggtt tgaatacctg
     4621 taccgcgcct tcttcgccaa gctgttgcaa acctttttgc agttgcttga ttttcagcgg
     4681 gtttttgatg cggacgctgc ggaacagttc gggcgcaaag aatgggatgc cggtaaacgc
     4741 cagttgttcg ccttcggaga agctgtcgcc gatttggatg ttgccgtggt tcgggatgcc
     4801 gatgatgtcg ccggcgtagg cttcttccac cagctcgcgg tcgtgcgaca taaaggttac
     4861 cacgctggag gcagcgattt cgcggttgat gcgcaggtgt ttcatcttca taccgcgctc
     4921 gaatttgccg gagcagacgc gcaagaaggc gatgcggtcg cggtgtttcg gatccatatt
     4981 ggcttggatt ttaaagataa atccggagaa tttcggctcg tccggcttga ccatacgtac
     5041 ggtcgcgtcg cgcggtttcg gcgcgggcgc ccagtcaatc aatgaattga ggatttcctg
     5101 aataccgaag ttgttaatcg cagagccgaa gaatacgggc gtgagttcgc cggcgaggaa
     5161 ttcgtcgaga ttaaactcgt tggaagccgc ctgcaccaat tcgatttcgt cgcgcaactg
     5221 ctggatttcc aatggaaagc gttgttccaa ttcgggattg tcgatgcctt tgatgatgtc
     5281 gaactcgtgc ggcagacgtt cgccaccggc ttcaaagaga tagatttcgt cgttcaggat
     5341 gtggtacacg cctttgaagt ttttgcccat gccgatcggc caggttacgg gcgcgcagcg
     5401 gatttgcagg atgttttcca cttcgtccag caactccaaa gaatcgcgca cttcgcggtc
     5461 gtatttgttc atgaacgtga caatcggcgt attgcgcagg cggcagacgt tcaagagttt
     5521 gatggtttgc gcttccacac cttttgccgc gtcgatgacc atcaacgcgc tgtccacggc
     5581 ggtcagaacg cggtaggtgt cttcggagaa gtcttggtgt cccggcgtgt ccaagaggtt
     5641 gacggtgtgg tctttgtagt cgaactgcat cacgcttgat gccacggaaa tgccgcgctg
     5701 cttctcgatg tccatccagt cggaggtggc gaatttgccg gtttttttgc cttttaccgt
     5761 acccgcgctc tgaatcgcgc ccgaaaacag caacagcttt tcggtcagcg tggttttacc
     5821 cgcgtcgggg tgggagatga tggcaaacgt gcggcggcgg cgcacttggt cgaggatttc
     5881 ttgggacatg gttttctttg caaaaaggtt caggccgctt ttcagacggc ccggacagtg
     5941 tttgagacgg cgaaattgta caaaaaaatg cctgataatt caatgttgga ggcggtcagt
     6001 gcgtgctgcc gtaaatctct ttttcgtctt tcaggacggc atcggcggtt tcccacgcgc
     6061 cgccgcgcca gactttgtaa aagcagcttt ctcgcccggt gtggcaggcg atgccgccgt
     6121 tttgggcgat gagcatcaca atggcgtcgc cgtcgcagtc gaggcgcagt tcgcggactt
     6181 tttgcgtgtg tcccgactct tcgcccttca tccattgttt ttggcgcgaa cggctgtaat
     6241 agtgggcaaa gccggtttcg acggtttttt gcagggcttc ggcgttcatc cacgctacca
     6301 ttaaaacacg tttggtttcg gcatcttggg cgatggcgca aaccaaacct ttttcgtcaa
     6361 atttgacggc ttcaagcagg tttttatcca tatttccttt cagacggcat agtcggggcg
     6421 gtcagaggcg cacttcgatg ccggcttcgc gcatagcgcg tttggcttcg cggatggcga
     6481 tttccccgaa atggaaaacg ccggcggcaa gtacggcatc ggctttgcct tcggttatgc
     6541 cttcaatcag gtgccggaca ttgccgaccc cgccggaggc gatgacgggg atgtcgacgg
     6601 cttcggcaac ggcgcgggtc agcggcaggt tgaaaccctg tttcgtaccg tccctgtcca
     6661 taccggtaag caggatttcg cccgcgccgc gtttttgcat ttcgatcgcc cattccaccg
     6721 catccaaacc ggtcggattg cgcccgccgt gggtaaagat ttcccagcgt gtgttttcgg
     6781 ggttgacggc tttggcatcg acggcggcga cgatggcttg cgaaccgaaa aatccggcgg
     6841 cttcgtcaat caggtcggga cgggtaacgg cggcggtgtt gatgctgact ttgtccgcgc
     6901 cggcattgag caggcggcgg atgtcggcaa cggtgcgtac gccgccgccg acggtcagcg
     6961 ggatgaagac ttgtccggca acttcttcga tgatgtgcag gatggtatcc cggttgtcgg
     7021 atgaggcggt gatgtcgagg aaggtcaatt cgtccgcgcc ttcgccgttg tagcgtttgg
     7081 cggcttcgac ggggtcgccc gcgtcgcgca aaccgatgaa gttcacgcct ttgacgacgc
     7141 gcccgtcttt tacgtcgaga caggggatga tgcgttttgc cagtgccata atcggatgcc
     7201 tttagtcgag ggaatctgcc agttgctgcg cttgggcaaa atcgatgctg ccctcgtaaa
     7261 tcgcgcggcc ggtaatcgcg cctgctacgc catgtttttc ggcggcacac agggcgcgga
     7321 tgtcgtccaa gccggtcagt ccgccggagg cgatgacggg aatgcggacg gtttgggcga
     7381 gtttgaccgt cgcgtcgatg ttcacgccgc tcatcatacc gtcgcgcccg atgtcggtgt
     7441 agatgatgct gttgacgccg tcgtcttcaa agcgttttgc caaatcaatt acatgatgcc
     7501 cggttacggt tgcccagccg tcgatggcgg ccataccgtc tttggcatcc agcccgacaa
     7561 taatcctgcc ggggaaggct ttgcacgcct cgcgcacgaa gtcggggttt ttgaccgccg
     7621 ccgtgccgat aatcacgtcg ttcagaccca aatccaaata ttgtccgatg gttttcaaat
     7681 cgcgtatgcc gccgccgagc tgtacgggga tgtctttggc gacggcggca aggatgtctt
     7741 tgatggcggg caggttttgc ggaacgccgg caaacgcgcc gttcaaatct accagatgca
     7801 ggcggcgcgc gccttgtttg aaccagtgca gtgcggtttc ggcgggcgaa tcggaaaaga
     7861 cggtcgcctc ttccatcagc ccttgtctca ggcggacgca gcgtccttct ttcaaatcga
     7921 tggcgggtat cagcagcata atttttctcc ttgtgcgggg ccgtgtccgg cttaccagtt
     7981 taaaaagttt ttcaacatcg tcagcccggc atcgtggctt ttttcggtgt gaaattgcgt
     8041 ggcgaatacg ttgtctttgc cgacgatgca ggcaaacggg gacgggtagt cgctttcgcc
     8101 caatatggtt tcgggatttt cggggtcgaa atagtagctg tggacgaagt aaaaacgcgt
     8161 gttttgggga atatctttaa acagcgggtg gttttgggtt tggcgcacgg tgttccagcc
     8221 catatgcggg actttcagac ggcatccctg ccggtcgcgg aggtcgcgct caaagcgtct
     8281 gactttgccg ccgaaccagc ccaagccgtc ggtgtttcct tcttcactgt ggtcgaataa
     8341 aagttgcgcg ccgacgcaga ttccgaaaaa gggtttgttt tttaaggcat ctttgactgc
     8401 ctcgtccaag ccgccgcgcg tcagtgccgc aatacagtcg ggcatcgcgc cctgcccggg
     8461 aaaaatgact ttgtcggcgc gggacacgcg ttcggggtcg ccgcttaaaa agatttcggt
     8521 attttttccg gcaagctgcc ccgccgtcca gacggatttc aatacggaat gcaggttgcc
     8581 cataccgtaa tcgataatgg cgatttgcat ggcttcctcc tctttttttg caatatggct
     8641 gcgattttaa caaacaaatg tgccgtgctg ataaaaatgc cgtctgaaaa cgggagtctg
     8701 tcttcagacg gcatagggtt taaacccgga aagccgtttg tcagccttcc atttgttttg
     8761 cctgaacggc agttagggcg atggtgaaca cgatgtcttc caccagcgcg ccgcgcgaca
     8821 ggtcgttgac cggtttgcgc aggccttgca gcagcgggcc gacgcttaag acgttggcac
     8881 tgcgttggac ggctttatag gtgcagttgc cggtgttcag gtcggggaag accaaaacgg
     8941 ttgcctgtcc tgccaccggg ctgcccggag ctttggattt gcccacgccc ggcacggttg
     9001 ccgcatcata ttgcagcggg ccgtcgatgg cgaggtcggg gcgtttttcc cgggcaagtt
     9061 tggttgcttc gatgacggta tcgacatcgg ggccgctgcc ggagttgacg gtggagtagg
     9121 aaatcatcgc cactttcggg tcgatgccga aggcttttgc ggaatcggca gactggatgg
     9181 cgatgtcggc aagctgttgc gcggtcgggt tcggattaac cgcgcagtcg ccgaagacga
     9241 ggacttggtt gggcagcagc ataaagaata cgctggacac gaggcttgcg cccggtgcgg
     9301 ttttaatcag ttgcaaagcg gggcggatgg tgttggcggt ggtgtgaacc gcaccggata
     9361 ccaaaccgtc cacatcattt tgcgccatca tcatcgtacc gagtaccacg gtgtcttgca
     9421 gttgcttgcg cgcgtcttcg ggtgtcaggc ctttggattt gcgcagttcg cacatcggtc
     9481 cgacgtattg ttcgaccaat gaggcgggat cgatgatttc caaagagtcg ggcaggctga
     9541 tgccgcgttc tttggcaacg gcttcgactt cttcgcgttt ggcaagcagg acgcagcggg
     9601 caatgccttt ttcgtggcag atggcggcgg cttggacggt gcggggttct gcgccctcag
     9661 gcaggacgat gcgtttgtcg gcttggcggg cgaagtcgat caggttgtag cggaattgcg
     9721 ccggcgacag gcgttttgct tcgcggcctg ccaatacgga tacgtctttc agcgcgtcgc
     9781 tcgaaccgaa gaaggtcagg ccggtttttt cggctgccgc ttcggcaacg gaggctgccg
     9841 cgccgtccac gacaaaacct tccaatacgc ccggcgcggc ggcgaagaac tgtttggcaa
     9901 ggttcagccg atttgccagt tcgtcggcat cggcgttgtc ggaacggacg gcgaagacgg
     9961 ctgccgcgtc aagggacaat gccagttcga cgtttttgcc tgcgaggtag attttgtcgg
    10021 catcgggcgc gatgccttcg atgacgaggt tggcggcatc gagtgcggca actttgccga
    10081 ccagtgcgtc gaaccagtcg tcgcttttgc cttgcgcgag cagggtttcg gcggttgcgt
    10141 caacggcttg gaaaatttgt gcgtccagtg cttttgcaaa ggcttgtgcg gcggcggagg
    10201 cgtccagtcc ggcagatacg ggtacgatga gtacttttgc catgatatat cctttcgtat
    10261 gctgcggtgt gcggcatatg tggttggaag gggcggcata taggcagaaa cggctgcctg
    10321 cgtgccgtgc gtgccgtgtt tggcttgagg cgcgcaggtt gaatatagca aacaaattct
    10381 gtttccaaca agataaatat ccgcaggctt gtggatgctg ccgcctttca gagggtattt
    10441 ccggggaaga acagggcggg accgtccaaa tggaggacgg cggaaatgcc gtctgacagg
    10501 gtgggggcgg aagggaggtt gagcgtgagg acggtttgtc cgacccggag gctgatttcg
    10561 gtatgccgcg ctttgggcgt ggttttgaga accacggcgt gaatggaggc ggtgggtgcg
    10621 gaatgggggt gaaggctgaa ctgttccgga cggatgagca gtgtgccgcg tgtgcctgcg
    10681 ggtgcgccgc tttgaacggg caggcggccc aatccgcaat cggcggtgcc gtcggcgttg
    10741 agcgcggcgg ggaacacgat gccttcgccg ataaacaggg cggcatcaag gtcggcaggt
    10801 tgtcggtaca attcgtgagg gcttgcagtt tggaggatgc gcccctgttt catcacggca
    10861 atccggtcgg cgtattgcag ggcttcttcg cggtcgtggc tgacgaaaac ggcagatttg
    10921 ccgttggcgc gcagggcggc aatcatgtct tcgcgaatct ggcggcgcaa ctgttcgtcc
    10981 agcgcgctga agggttcgtc caacaaaatc agttcgggat cgggtgcgag ggcgcgggcg
    11041 agggcgacgc gctgttgctg tccgcccgaa agttcgtgcg gatagcgtcc ggcaagttcg
    11101 gaaatgccgg tcaattccaa catagcttcg atgcgctgcc gctcttgcgc cgtcttgcct
    11161 ttgccgttgc ccagcccgta ggcggtgttg cggtaaacgg tcaggtgggg gaacagcaca
    11221 ccttcctgta cgacataacc caaacggcgt tcgcggacgg ggaggttggt atttttcgag
    11281 aagatggttc tgccggaaag cgaaatttcg ccaaaatcgg gttgttcaaa accggcaagg
    11341 cagcgtaaaa gggtggtttt gccgcagccg gacgcgccga cgataaagag gatttcgccc
    11401 gggtcgaggc tgagcgaaat gtcgtttaaa accggggtgt tttgaaaact tttggacagg
    11461 tgtccgatgt gcagggcggc ggtcatggcg gtacttcctc aagctgttat ttgaaggcgt
    11521 atttcttcag caggaatacg gggatgccgg aaaataatac cagcatcagc gcgtaagggg
    11581 tggcggcggc gtattgtgcg tccgatgtgt attcccaaac ggcggtggag agtgtgtgga
    11641 catcgtcggt ggtcagcagc agggtggcgg tcagctcttt catcagtttg aggaagacga
    11701 gtgcgaatgc ggcggtaatg ccgggcagga tggacggcag taccaacgtc ctgaaaataa
    11761 agaagtgtcc gcgccccaat gttgcgccga cctgttccat ccctttgggg agttgttcca
    11821 aggaagtcct cagggtggtt tgcgccatcg gcaggtaaag catgaaatag gcaaggatga
    11881 cgacgataaa ggtttggtaa acggcagggg tgtagttgat gctgaaataa accaaggata
    11941 gggcgataac caaaccgggg acggcgtgca gtaaaaacgg cagcctgtct atccaaacgg
    12001 ttaaaaaatt gcgatagcga accgatgccc aaacaagggg caaggcacat aatatagtca
    12061 aaatcgcacc taaagccgat acgcttaagg aacggataaa ggcatcaaat acggatacga
    12121 gcgcgaatgt gccggaagtg ccgaccatca tccaatgtat caatacgcca aaggggataa
    12181 taatgcccaa agtcaacaag ctgcttaaaa aaacaatcgc gccaatctga ccgggcagtt
    12241 tgagggtttt gacgggataa ggacgggcaa cgcctttgcc gctgtggtaa atcttggctt
    12301 tgccgcgaaa tatgctttct ccaaatacga cgatgccgca caccgccatt aaaacagcgg
    12361 aaagcagggc ggcggtattg ttgttgtagg acatttcgta ttcttggaaa atggcggtgg
    12421 taaaagtggg gtagttcaaa atggataccg cgccaaattc gaccagcata tgcagggcaa
    12481 tcagtaacac gctgctgccg atggcgggtt tgagctgggg gaggatggcg gaaaaaaagg
    12541 tttgcaggcg gcttttgccc aaggacaggc tgacttcttc gtaagacagg ctgatgcgtt
    12601 tgagtgccgc ctcgacgggc aggtaggcga gcgggaacga ggacaggctc ataatcatca
    12661 ctgtccccca aaagccttcg acacggaagg tcaggctgat ccaggtgaaa cagctgacaa
    12721 atgcggggat gcacaaaggc agggtgattg ccgtctgaaa aaaggttttg ccgaagaagc
    12781 ggtaacgttg gaacaaaagg gcgcaggcaa tgcccaaaac aatggaaatc agggtaacgc
    12841 ccgccatcat cgtcaaggtg ttggagagca aatcccacat acgcgggcgg aacagtagtt
    12901 cgacggcgcg gttgatgccg acctgccacg aacgcatagc gacatataaa aaaggcaggg
    12961 taagcggtag ggcaatcagt aggatgaggc cggtaagcca aatgggtatt tttttaggag
    13021 acatagtgtt ttttatcggc aaaacgggcg gacagtataa atgtccaccc gtttgacaat
    13081 ccgaaaacgg cttatttcat accggcttgc tcaagcagcc gggtggcgtg ttctttttcg
    13141 gaaacagtgg tggcggacac ttggggtgct tccaacttgg cgatgggttc caaattgaag
    13201 gtggatacca cgtgcggatt caaaggatac tcggcacgga cggcggtcag ggcacgctgt
    13261 ccttccttgc cggcgaggaa ggcgacgaat tttttcgcct catccttgtt ttgggaggat
    13321 tttaacacgg ctgcaccgga ataggtaatg agtgcgccgg gatctctgtg gcggacgaaa
    13381 ttcaggcggg tgtggacgtt ttgtacgcct ttttcacgcg cgaaagcgtg ccagtagtag
    13441 ttgttgatga gggcggcatc gatttcgccg ttttcaaccg cttgaagggc gacggagttt
    13501 ttagcgtaag gcttgccgta ttctttcaga cctttgagcc atttcaatgc ggccgcttcg
    13561 cctttcagtt tgacgatggc gacaacctgt tccaagaacg cgccggaagt gggggcgtaa
    13621 ccgatgcggt ttttccattt cggcgtggcg taattcagga cggatttttc taaatctttt
    13681 tcagacagtt tgcgggtgtc gtaaacgacg acgcgcgaac gtccgctcag tgccacccag
    13741 tcttttttgg cggcaaccgg cacgcccttg ccgcgtgttt cgttgatggt ggaggcgggc
    13801 aggggctcta ggaggttggc tgcggaaagg gtggcgagtg ccgggatttg ttcggaatag
    13861 aatacgtcgg cggggcttcg gctgccttct tctttgattt gaccggcaag ctggtcgcct
    13921 ttggcgctgt tgagtttgac tttgatgccg gtagcccggg taaaggcatc tgcaacggct
    13981 tgtgctgctt ctttgtgttg tccgttgtac acggtaatgt ctgccagcgc gggggttgcg
    14041 gcggtcaggg ctgcggcaag cagtgcgtat cggatagatg ttttcatatc gattttctcc
    14101 taatgaatga gagtgtatac cttgttaaga cataacggtg tgtagtgtat tccttctttt
    14161 ttataaatgc aaataattat tttttaaatt tgttgttgtc cgatccggtt attgtttgtt
    14221 ctgacttgta ttttttccgt gagtctcgcc cgtaaggcgg aagtggcggg caatgcgtgg
    14281 cggaatgtgg gtaaaggcgg cattttgatt tgtcggaatg cttgagaacc cctctcttta
    14341 aaac
//
LOCUS       DAWHRA010000042        14280 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWHRA010000042 DAWHRA010000000
VERSION     DAWHRA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.042"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(26..1999)
                     /locus_tag="WIB30_000934"
     CDS             complement(26..1999)
                     /locus_tag="WIB30_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ3797019.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVITGDQTVNGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIQGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     ALGYEDRNERQRSALVSTWTDENLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSERVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(2010..2570)
                     /locus_tag="WIB30_000935"
     CDS             complement(2010..2570)
                     /locus_tag="WIB30_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ3797020.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQHRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(2567..3178)
                     /locus_tag="WIB30_000936"
     CDS             complement(2567..3178)
                     /locus_tag="WIB30_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ3797021.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLEIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVSSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            complement(3178..3621)
                     /locus_tag="WIB30_000937"
     CDS             complement(3178..3621)
                     /locus_tag="WIB30_000937"
                     /inference="COORDINATES: protein motif:HMM:NF016736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ3797022.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLSRRNPTTASGTLTVSGIAQSMLSDD
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVATARRN"
     gene            complement(3635..3982)
                     /locus_tag="WIB30_000938"
     CDS             complement(3635..3982)
                     /locus_tag="WIB30_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ3797023.1"
                     /translation="MDKELNPGTGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            complement(4083..4751)
                     /locus_tag="WIB30_000939"
     CDS             complement(4083..4751)
                     /locus_tag="WIB30_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ3797024.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(4748..5892)
                     /locus_tag="WIB30_000940"
                     /pseudo
     CDS             complement(4748..5892)
                     /locus_tag="WIB30_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(5882..7213)
                     /locus_tag="WIB30_000941"
     CDS             complement(5882..7213)
                     /locus_tag="WIB30_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ3797025.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGTTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(7213..9384)
                     /locus_tag="WIB30_000942"
     CDS             complement(7213..9384)
                     /locus_tag="WIB30_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ3797026.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPDTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALGWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            9539..10141
                     /locus_tag="WIB30_000943"
     CDS             9539..10141
                     /locus_tag="WIB30_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797027.1"
                     /translation="MNMKKLISAICVSIVLSACNQQSKTAQAEEPVQSIQAADCTAPM
                     DITVEQYLINLEQAFKTQNVSTKIHNKNIVKTDCGYDLTLVMDFGAIALKLDEQQKIR
                     AISVGYILKTDGEKGQNLVNNAINGLHSIQAVLSLTTTDKLGESEAGKQLFTALTEVV
                     KESNQTGATAQKDVPADGILYSVVFEKETNTIAIIGRKQP"
     gene            complement(10185..10418)
                     /locus_tag="WIB30_000944"
     CDS             complement(10185..10418)
                     /locus_tag="WIB30_000944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797028.1"
                     /translation="MRRRRRSSKTQLPDGTQKLPSGGHPDGKAGIGADAVRDMCHAEL
                     SAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(10393..10758)
                     /locus_tag="WIB30_000945"
     CDS             complement(10393..10758)
                     /locus_tag="WIB30_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797029.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     RLKPSAATASPDAPSAAEQ"
     gene            complement(10762..11139)
                     /locus_tag="WIB30_000946"
     CDS             complement(10762..11139)
                     /locus_tag="WIB30_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ3797030.1"
                     /translation="MSDATYADAVIMEMNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEDRRTSYLDCFTVDTG
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(11204..12613)
                     /locus_tag="WIB30_000947"
     CDS             complement(11204..12613)
                     /locus_tag="WIB30_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ3797031.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVADRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANAFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVSNAIEQRGCIGVLGMSAALSTATTATGEINDGRM
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPLFAECNNA
                     LYNGLTPLTVVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(12616..12813)
                     /locus_tag="WIB30_000948"
     CDS             complement(12616..12813)
                     /locus_tag="WIB30_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ3797032.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(12810..13460)
                     /locus_tag="WIB30_000949"
     CDS             complement(12810..13460)
                     /locus_tag="WIB30_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ3797033.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(13450..13872)
                     /locus_tag="WIB30_000950"
     CDS             complement(13450..13872)
                     /locus_tag="WIB30_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ3797034.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(13872..14270)
                     /locus_tag="WIB30_000951"
     CDS             complement(13872..14270)
                     /locus_tag="WIB30_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797035.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
BASE COUNT         2768 a         4136 c         3891 g         3485 t
ORIGIN      
        1 tctctacagc catctctaca gcccgctagt cttgcagtgc ctcgagcaag ccgtctcggc
       61 ggcggcggtt tcggggtccg gttgatgttc ttccactctc tctttgcggt tcttcgttgg
      121 tttcaggtat ttcggggcct atcggggagc tggcggcgtt tcccgaggcg gctttgccga
      181 tggccatcca gtcgcaactg ccttcgtacc agttggtgat aatggcggcg gcgtggtttt
      241 ttgcgcggat aaacagccag ttgtgttgcc ttcgatcgcc ggtaacgcgt tccgaatggc
      301 gttcggatac gaagcatttg acgttgccgt cggcaaaggc gacggggaag acgacttcgt
      361 tttcgatggg gccgccgctt cgtgtgaacc tgtagctgcc ggtctgtatc attgtgccgt
      421 cggggaatct gacaatatcc acgccgccga tggtttggcg tgtgaattgc gcggcaacgg
      481 cggtgctgat tttttcgtcc agcccttgga tttgggcggc ggtgtgggtg tggtctcggt
      541 cggctttgtc ttgcaaacct tgcgccagcg tgcccgcgtc cagcgcgcct gcgttgatgg
      601 tttcgccgtg ggactttatc caaaacacgg cttcgcccaa ggtgtcggcg gctttgatgc
      661 acagttttaa aaccagggct ttggggcggt tttcgtcgcc gcctgtcgcc attttgctgt
      721 ccgagcgcgg ggttaaaaag ccgttgtcgt ttaaattttc gtccgtccaa gtcgatacga
      781 gcgcgcttct ctgcctttcg ttgcggtctt cgtaaccgag ggctgccgcg tctgtgtggt
      841 ttgtccagtg tgaaaatact ttgtggacgt gccgtttgat ttcgtcttcc tgcttcgttc
      901 cgactgccaa gctgttgccc gcgttgcgga taaagcggtc ttccgcctgc gggacgtttt
      961 ggatgctgcc gtattttccg gtcagcagac ggtacagctc gggataagcg gtttcggtta
     1021 cgcgcgtgcg gatgtcgtca aacgccagcc agccggtcgg gatttggtcg gacggaaacc
     1081 acgcggtgat gccgatgtcg gtacggctta aatcgggcag gcggttgctg ttgcccaagg
     1141 cgcggtaaag gtcggggaag gtgttttgtg caaaggtcgt gccgttggct ttgagatagc
     1201 ctgcggggtt ttgtacggct ttgggaaagg atacgatcgc gccgacgggg atgccgtccg
     1261 aattgagttt tttccatgcg gaccagttgt cgtttgcgct gctcgtttgg tggcgttcgt
     1321 acacgtcgga cgtgtaggcg ggataaccca actgcctgca ccagccgtgt ttcgtgccgg
     1381 cgatgacttg gatatggcag gcggtgtttt caacgggcag gttggagctg ccgaccgccg
     1441 tcggcaggga atagatgccg tctgttttga gggtgttcaa atcgccccgg aatgtttcga
     1501 ctttgaaatt gccgatgccg tatccggaca gtgtgttcgg tttgccttgg atgtcttcgg
     1561 caaaacgcgg tttgttggct ttgtggaaga tgtcgtggtt gttgtaggaa atgcggttgt
     1621 ttgcgccgtg caggcggagg gtgtcgtcgc cgaagacgat gtgggcgttg tcggcatccg
     1681 cgccgatgta ggctttgtgg ccgccgttcc agttcgtctg gttggcggat aaatggatgc
     1741 cgctttggaa ttgggtttgg gcggtaaagg ttttttggcc gttgacggtt tggtcgccgg
     1801 tgataactac agccagccta tttagtgctg ccagtaattg attaggctgc gacttatccg
     1861 gctggatacc ggccgcagtc agcacggcca gcagctcgga ttgcacttgg ttgagccacc
     1921 acgcgggcag gatggtgccc aattcggaca cgccgtcgcc gtcgtggaag gtcttatcgg
     1981 gggtttcgat ggggtgcata aatcgtcctt tagcggtagg taaatcggat agcggtgtgg
     2041 gcgggcttga ggcggttgaa caggctctcg atcacggcat cgctgtaatc ggtcagcctg
     2101 tcgcccgccg ccgagatacc ggcgcggaat cgggtaatgc ggttgttgcc gccgcgcacg
     2161 ttaacgtgcc acacccacat gatttcctgc ggcgcaagac ggtcgccggc gcggtttaca
     2221 ccggcgcgga acggctgcgg ttcgtcgatt tggatttgat agcccgccgc ctcggccaaa
     2281 cgcacaaaat aaggaatact caagccgcct gtttcgttta gcttggccat gacggccaac
     2341 acacggtgct ggcggttttt gcccgtaccg tccaaaccta atacgcgctc ccaatcggcc
     2401 agcatttggc cggcgctgcg cgggtcgacg gcatcggcaa cgctttgagc cgattccgcc
     2461 accgcatcca gcgctgcgcc gtctatttct gcctgcgccc gcacacgcgg ggcattgcgg
     2521 gcatacgaca cggggggcaa caggccccgc aagatgtctt gatagctcat gacggcatct
     2581 ccgttacatt aatcgcgccc gctttaaacc actcgatgcg gttaaccgta tcggcggcac
     2641 ggttggccgt cggcgcagtc agacggcggt cgatcacacc atccacattg ctgatagcag
     2701 cctcgatttg cgacacagtc aggccgtcgc cgggaatcag ggtgtcgaaa tattctttta
     2761 gggccgtccg tatgcggcgc ttggcctcgt ccaagtcgat accgtcgagc ttgacttgca
     2821 cggtaacagg caccgccgtt acggttggct tgagtaccag cgcatttttt gccgttaccg
     2881 ggcgcatctc gtcgatataa gcctgtacgc ggcgcacagt ttcttccgac gacacaccgt
     2941 cggcggaggt aatggcaata tccaccgtac ccaagccgcg gcgcagcgga taaacatatg
     3001 cgctggttac gccgtcaaca ctcaacgccc agtttttata gtcgtaacgg ttgccgcctg
     3061 cgggcggtcg gcggatgatt tccaacagac gcgccagcag tgaggcatcg ctttctcggt
     3121 cggtgccgcc ttgtacggta aggcggcatt cggtggccac accggcgggg gcggccatca
     3181 gttgcgcctc gccgtcgcca cattggcggc cgcgcccggc tcgtcggcga ttgccggtat
     3241 ttccgccgtg ccgccgctgc cgataacggc gcgggcggta gtgcggtaaa aacgctggcc
     3301 gatacgcact tgcaggtcgt ctgaaagcat ggattgcgca ataccgctta cggtcagcgt
     3361 gccgctggcc gtggtaggat tgcggcggct caagccgcgc atggaggcat gccgctccaa
     3421 atactcgcgg tcggcggtat cagggaaaat ctgccgcaca atccagcttt gatgcgcata
     3481 ttgcccttcg gcgcagctgg ccaaacggct ggcgtgcaca taatggtcgc tgtcggggct
     3541 gatatcggca tccggccaca ggcttttggt atcgcgcagg atacgctcgc ggatttgctc
     3601 aaatgtcggc gtttcaaaca cgtttaaaac ctctttaaat cacgggcact tcgtggcggt
     3661 aatcaaaccc gcccgccgcc gtatccaccc ggatatgcag gatcaggcgg ccgtcgtgcg
     3721 gctgctctgc gcgcacggta atcttgtcgg cacgcccgct cttaacaatc ggttgcagtg
     3781 cctcatcggc atattgctcg gccaacaggc tgacccgttg caggtctttt tcgcgctgca
     3841 acaaatgcag cagcgagccg agcgttttat ccgcccacca gctgcccaac ggtgtcatca
     3901 aacggatata cacggcattt tgcagcgtat cgacggtgcg gccggtatag tcgccggtgc
     3961 cggggtttag ctctttgtcc atgttggcat tttgccgttt tcagacggcc tcacggagta
     4021 cacgggcttc aatcccgcac gcacaaaaaa agcagcccga aggctgccaa aggtctgctt
     4081 gtctatgccg gttccgctgg taaggttttg ccgccgatgc tgtcggtatg cgggtgctgg
     4141 cgcaacgata tattgccggc caccacgtcg ccgtcggtgt taaagctgcc gcccgtttgg
     4201 ttaacatcgc cgctaaaggt ggctccgtcg ccgccctcga cggccatgcc gccgttgccg
     4261 ttgatttggc cttgcgccgt caacactgca ctggtctcca ccaacggagc gttaaatttg
     4321 gcatccgtgg ccgcattaac ctcgtattgt ttgcagttaa cccggtacac gtcgcaatcg
     4381 gcctcaatga ttttgccttg cttaatcacg atttttgcac cctcatgatt aaaaatcgcc
     4441 gtctcgccgg gcttaaggtt tttgatgcgg tagctgccgt gctggctgca cacaatcaca
     4501 ccgtgcgaag tattgccgcc cagcggtatc actaccgctt cgctgccgtc gggcggatgg
     4561 ctggcaaagc cgtattcctg caaatgttca aggtcttgca gggtttcgtc ggccaagccg
     4621 ctcaactgca cgcgctgtat cggctcggac gacaccacca gcgtgatttt tccccgaaag
     4681 gccgcgcgca gggtttcgcc gatattttta gcagtttgtg ccgttttttt cgccaattta
     4741 ctcaaactca ttcaaacacc gccgtttctg cttgtttttt gccgcctttt ttgcccttat
     4801 ggctcacgcc tttgcgtttg cctttgcgct tgcgcgccgc ctcggccttt ttggggtaag
     4861 cgtcgggtgt ccaaataccg tcctctttga gccgcagctc ggtttgcgtg ccatccatgc
     4921 gggatagcat aaaccgccgc cccatcagaa aaaacaccgc atcgataccg tgctcgtcgt
     4981 cgatcacatg cacacgctgg ccaggttgcc acaatacgcc gtcgcgggtt ttatggccgc
     5041 ccacggttat cgtgagtgtc aatccctcca gccgccagtc ggccagctgc tttttagcct
     5101 gcttttgcaa tgcggccaaa ttgtcggcat cggacaccac caccgtttta gggcggtgca
     5161 gcgtcatcgt cgggtctttg tacacccact taaatcgtgt ttggcgctgt cgccgctgcg
     5221 gccgtgcgat tgcgccaaaa aagtaacctc ggaaaagcgg ttgtcggtat cccactcaat
     5281 gtccatgcgc tcgatattgc ggcggctgtc ggtgcggctc caacacaatg tcgccaccgg
     5341 cgggctgctg taatccgcac cgcccaccac caacgtgccg tccggctcca gccacggatg
     5401 cagcccgacc gagttggcaa tatgggttaa tgcctgccat acggtttcgc ccggctcgat
     5461 gtcgattttg tccaaagcgg ggttgttttc ggccttaagc accaccgctt taatctgcgg
     5521 ccacggcgcg gccagctttt tggctgcatc caataccgtc atgcccttta cattgagctg
     5581 cggcgcggag caatccacca aaaaaccggc caaatcacgc ccgctcaagc tcaactcgcg
     5641 gccgcccttg cttttgccgt ggcgctggct gccgatgatg cccgtcatca cgatttgccc
     5701 gtctatcact acctcgcagc tctctccgct taaatcgggt atggccgcct ccggccccaa
     5761 cctgccgatg acaaaatcga agctgtcggc agggattaaa aagtcgctgt cgatgtcgta
     5821 gcgctcccag tggcggtgct ctttaccgcc cacgcgcacc gacacggcat agccgtatga
     5881 attattttgc atagctgttg accaaagtgc cgcgcttgat aaacgcgggg tggtggatat
     5941 ggggattgag ccgcaccagc tctgctgcgc gggctatatc gccgtaaaac tcgtgggcga
     6001 tttggtgtat cgtaccgtcg attggggctt ggcgcacaat cagcggcggc ttttggttga
     6061 tgaccgccgc aaccaacgca ttcagacggc ctgccgccgc gcgcagggat tctgccgttt
     6121 ggtaagcctc ggtatacacg gcgttggccg tcagcccacc agactcggca gcagccgtct
     6181 gcaccgcccg taaggcggca atctcggcct gcatacggcg gcgcatggcg cggttaacct
     6241 caatcaaatc gggcgcggtc atctcttcgc catgcgcctc gattaatgcc gttgccaccg
     6301 acaacagcga tgacgtggat aacaggcgca ccgcctgcgc taccggctgc acctgtttgg
     6361 cggttaaacg gttcaggcgg ttttgcaggc cgtctgaaaa gcggccggtc agcaaattat
     6421 cagggatagc ggcggcacgg tcggcaacag ccgcagcccc gtcaaaccgc tgtcgcggcg
     6481 accaaccggc atggtgcatg gcattatcgg ccaaacccgc ctcacggcgt atgccagtat
     6541 ctaccatgac cgatatatcc gcaaacagct tggccgagcc gtttttaaac gccgctgcgc
     6601 tgtatccgcc ccgatcggga aaggcaattt tgtccaaatc aaacaaacgg cgcaccgcgc
     6661 caaacgtacc cgatgccgca ctccaaatgc ccagcgcgct gccccataaa gccgatacgc
     6721 ccgcatccac cgccaacacc gcatcaacaa agccgatagc cgcctcgcgg taggtgtcga
     6781 tatcagcaat caacgcctca agctcgacca aaaaggcgtt ttcaaaaaca aagatttcct
     6841 gcgcttcggc cgcctcgcgg aaagtaatat cgatgcccgc ataatccaca taatcggcct
     6901 catgtcggta gctccatgat gccgcaatca tgttgtgcat ccgcccccaa acagggtgca
     6961 ccagcacgcc gccgcccggc tgctccagcg catccagcag ctttttcaga cggcctgcat
     7021 agcccttgcc ccaaaacacc gcattaatct gcacctgccg cccggtcgtg cccatgtctt
     7081 caaggtcgat accctgcaca aacggccgcg catgctcggc caatgccttg ccgttgctct
     7141 cgtccaccac ctcaatatca aagccgacgc ccttgtaaga tgcgtcctgc aataaggtat
     7201 gccatccgct cattattgac ccgctccacg gccgaacatg gccacttgat accgcgatac
     7261 ctcattcgcg ataacacgtc cgtcgatatt caggctcaca ttggtattga tggtctgact
     7321 tgccgacgcc atcgcacttt gcacttgtgc caaacctgtt gtaactgcag ccgtatcctg
     7381 cgccaatgct gcctgatacg ccgccgtctg ctgctgaatg gctgcctgat acgccgccgt
     7441 ctgagttgaa aacaacggag acaacatctt atcggtaggc tgcgctgacg gagcagccaa
     7501 atactgaggg acaccacccc gcgcctgttc ccgcttcggc gcaggttcaa atcggatacc
     7561 gttgtttttc agacgattaa tcatttcagg agttgtcttg gtaccacgca aaccctttgg
     7621 caatgtgccg tcacccaaag actcggaata cagcaacata cctaacaaac ccgcccgttt
     7681 aactaacgct ggattattca tgagcccgct accggcagat tttccccatg acagcaactt
     7741 accgcctgcc gctgtggcac ctgctgccac gccgcctgcg gaagccttac cccatcccaa
     7801 cgctttacta cctacatctt tcagaaagcc tgcaccttta ccgccaccgg cggttaataa
     7861 cattgcggca gacgccgctg ttgccgccgt cgtcaatgct tgcagggcta gagtggcatt
     7921 agggtattcc gctgccagct ttttaaacga ggtttccata tccaccaatg ttcccgtcag
     7981 actttcccgc aacattgcca gcgattcctg ttgctcttgt tgcgccgctg ccgacaacat
     8041 tcgcgcctcg ttgttcttag caatttttcc gttatccaac gttacgccag ccaacgaagc
     8101 catatattca cggatttgcg tcatatccgt tgcagccagc aaaccttgtt ttgcctgcaa
     8161 atcaggcagc agttgcgcca aaagcgcgcc cttaagcata tttgcctgct ccgccgccgt
     8221 cttatcgcct gcagccgcgc gtttcttata atcttggtat tgcttatcct ttactagcat
     8281 ggcatcggca agacgggaca acacctgcac tgcgttttcg ccgttttgct tgccttgcac
     8341 aaccgagcct atccaatcga cacctttctt cgggtcattc ggatttgcca tcttcttcag
     8401 acgacctatc gtgtcaggcg acagagtttt actcaaaaga ttttgcacat tagtcgccgc
     8461 ttcggcagga ctgcccgatt tattcgccgc agattgtaag agtgagagca ggtagtccaa
     8521 accgccgaca ccattcatcc ctgcctgttg cgcggcaggg agcaggctcg gcagctcccg
     8581 aaccatatcc cgcacctcga aagtgccgtc taaacccgat tgcaagacgt gctcaagccc
     8641 gagttgcagg tctttaccgc tcatgccgcc atctttcagg gttttaatca gttttgccgt
     8701 atcctcgcca ctgccttctg aggcaagtgc aaaagcatat gccgcctgcg cttcattctt
     8761 ggtttgggca aaattcagac tggtagtcat catgccgctg attaaatcca aagccttatc
     8821 gtgggtcccg ccatttttct cgacaagttc aagtgccaaa tccttgatct gttgcgcacc
     8881 ttcagttgca atccacgctg ccgatttact gttatcctca ataaatgcct gtctggacac
     8941 gcggttgatg ttctcatcaa gctgctttct gttgtccata gcaggcttaa gcaccgtata
     9001 cgctgccgcg ccaccagcca ccgcagctgc accgaatctt ccgatttttc ccatcttgtc
     9061 cgcaaatccc gtgccctgtt tcagttccgc gttcagctcg cgaattcggt tacgcgtagc
     9121 gaccgccgcc cgtgccaaat cattttgtga tgccttgccg ctgcgcgcca aacggttaaa
     9181 cgcagcctgt gtcagcatga tttcgcgttg aatctgtttt tctgagcgga tgccggtcaa
     9241 cgcataagta tggctttgcc gttcaagttg cgcccgcgat ttcgcggtac gatctaattg
     9301 tcgttgagta tcagccagta gccgtctcgc tccgtcatct cgggcggtta acaccaacga
     9361 cagtttcatg ttcccgtttg ccataaaaag cagcctttaa acgtattaac tcaaggtcaa
     9421 ttatcgttta aaggctgttt aagggaggcg gtatgggctt caaccccgtt ttcgtgcttt
     9481 atttgtttgt tgcgttataa tactagaatg ataaccattt tattcaaagg gatattttat
     9541 gaatatgaaa aaattgattt ccgcaatttg tgtttcaatt gttttatcag cctgcaacca
     9601 acaatcaaaa acggcacaag ccgaagaacc tgtccaaagt atccaggctg ctgattgtac
     9661 cgccccaatg gacatcacag ttgaacaata tctcatcaat ttggagcaag catttaaaac
     9721 tcagaacgtc tcaacaaaaa tccataataa aaatattgtc aagaccgatt gtggttatga
     9781 ccttactttg gtaatggatt ttggggcgat tgcgctcaaa ctggacgagc agcaaaaaat
     9841 tagagctatc tcagtaggct acattttaaa aaccgacgga gagaaaggac aaaatctagt
     9901 caataatgcc ataaatggat tacacagtat tcaggcagtt ctgtctttaa ctaccacaga
     9961 caaattgggc gaatcggaag caggaaaaca actttttaca gctttaaccg aagtcgtcaa
    10021 agaatccaat cagacaggag caacagcgca aaaagacgtt ccggcagatg gtattttata
    10081 tagcgttgtt tttgaaaaag aaacaaacac cattgcaata atcggcagaa aacaacccta
    10141 accgtcaaat aaaaaaaagg tcgtctgaaa ctcagacgac ctttttattt tccctccggc
    10201 tttctcagcc gtttcgacac aatcactccc tcttccttcg gcgtttttat gcccaggctc
    10261 gacaggatgt cttcaaacca tgccgacagc tcggcgtggc acatatcgcg cactgcgtcc
    10321 gcccctatcc ccgccttgcc atcaggatga ccgcctgacg gtagcttttg tgtgccgtca
    10381 ggtagctgcg ttttactgct ccgccgccga cggcgcatcg gggctcgccg tagcggcgga
    10441 cggttttagg cgcaaatcct cctgagccgc catcacggta tggtagtctt cggtagagag
    10501 gttttccatc agccactgcg ccgtcaggat gtcggatggg ataccttggg cttcaatctg
    10561 ctgcgaccag taggtcagcg tctctaggac gtcgcgttgc agcagttcgg cttttgtggc
    10621 gttttcgggc agctcgggca agtcatcaat cagggcgaac gccgccagtt cgccgccgac
    10681 ggtcagtggc ttgaggcgta attcagatac gactttgcca ttgtaggtca gcccgtgggt
    10741 cagtttgacg gtaatcacgc attactcctt gattttgtgc aaagcaatca tctcaatgtc
    10801 gatgcgtgcc tcgttatcga cttcatattg ctcgccggta tcgacggtaa agcagtcgag
    10861 gtaggaagtg cggcgatctt cgtcgttgat ggggtaaatc gtgattttcg ccttggtgat
    10921 gttgtcccag tcgatttccg taccgtcgat cggaatggcg gcggtaacgc ttaatttgtg
    10981 ttcggttacg ccgtcacaat aaccgttgac tcggccgttg cggttcatcg ttttgaccgg
    11041 cttgcggcct gtagtggttt gcggcttgat gctcacaatc tcgatatcgc ggccgttcat
    11101 ctccataatc accgcgtcgg cataggtagc gtcgctcatg ctttatcctt ttttaaggtt
    11161 tcagacggcc tttaaacctg ttttaaacgc cgtctgaaaa gggttacaaa atcaaatcaa
    11221 tgcgcccggc aaagacgtgc aggccgttga ccacatcggc ggggataatg gcattaacac
    11281 ggttggggtc gttttgcgca cgcgccacca ccagcttgcc tttgttggcc tcggcgtttt
    11341 cgatgatttc ggcttggtcg agcttaatca gcacgtccaa aatctcgctc ttaaccttgg
    11401 gcagcaggcg gtcgctcaat ttgtcgcgcg gaaaacgcag ggcaatgcgc tctttaacgc
    11461 tgcggcgcac ataatccagc gtgcggatgg tggtaatgtc gagtagtgcc gggtcgtcgg
    11521 tgttgttggc cgacttggta taggtggata cggcacgcat aatctgcacg cggttgttga
    11581 ccactgtgag cggggtcaag ccgttgtaca gcgcattgtt gcattctgca aacagcggcc
    11641 attgcgcatc aggtgtaacg gccagccctt tgatttccag cgtgttcagc ggcttggcag
    11701 ggtcttcttc aaaggccaac accgccgcat aacctgcggc gatgatgccg tttggctcta
    11761 ccgcaccttt gtaccaagca caggtcatgc ggccgtcgtt gatttcgccg gtagcggtgg
    11821 tggccgtgct caaggccgca ctcataccca atacgccgat acagccgcgc tgctcgatgg
    11881 cgttggatac gttggtaata tggttgctca aggctttggc gttggcgtca tcgctaaacg
    11941 ggctgcaaat aatgtgataa tgcttgcccg ccactttgga caaggccgtg gcaatgtccg
    12001 catttttggc accgccggta aaggcattgg cttgataagt taggccggta ttgccggtgc
    12061 tcacggttaa gccgctctcg ttgccgatct cgcctttgtg tttggccgtc agcgtaacgg
    12121 ctgcgccgct gccggatgcg gtaacggtta catcggcggc agtgatggcg gttttcagac
    12181 ggtctgccac tgtggcggcg gtctcgccgg tgttaacggc cgtgcttacc tgcttgccgc
    12241 caatcgtgat ttccaccacg cccggcgcgg tggccgtgcc ggaaagggta acggttgcgg
    12301 ttgcctgcac gcctgcgctg tggtcggcaa taccgataac ggtcaaatcc aaataagggt
    12361 tgttggcaaa tgcttggcgc accatcaaat gcgccagcga gccttgtccg aacaaatcgg
    12421 ccgcctcggc atcgctaaat agttgcaccg gctctaaggc gggctgtatg cccgcggtca
    12481 gcatgggtgc aaccatcaat accttttgcg gattttgcgg caaaccttgt acggcattgc
    12541 gggtgttaaa ttcaatatac tgcccgggca cgcggatgct gcccggaatc gtgtcaaaat
    12601 caatatgggg catcattact ctcccttagt attgcgggtt ttggtatttg gggcggcatc
    12661 ctcaaccacc accaaatcgc cgtcatcaat catgcggcgg taatacaggc tgttgccgtc
    12721 cacctccacc ggctcttggc cgatatattc gtgcgggttg tgttcggtcg gcacacgcag
    12781 gccatctgcc gccgttactt tgattttgct cattgcttat ccttaagggt taggtttaca
    12841 ggtatttcat cggcggattg cgggtcgtaa attttgccgt ccaacccctc gaaatcaggc
    12901 cacggctcgc tcaatgtacc ctgatacttg gtaaagatat ggttggggtc gtcgggattg
    12961 tcggtgcgtt cggggtagcg gtcattttcc aacccgcagg tgttaaagcg gatggcatac
    13021 tcaaccgcat atatgcttac tgcggcgttt tgcaccagca catgattggc aatcgcgcgc
    13081 accgctttgg gcaccaagcc gcggctatcg gcaaaaccga gccgctggcc gtcaagcagg
    13141 cggcgaacag cgcggattaa atcgttgctg ccgatttcgc ggctgtcgat gccgccttgc
    13201 cgctgcgcct gctcgttgcg cagattgcgg gccgccacca tcaccacaaa ttcggcggta
    13261 tcctgataac gtccgcatac gccgccggtg ctggcaggct caactttgct gccgccatac
    13321 gttacccaaa ccgcaggcag cgtatggatt tgccccgcca aatcgtcggc ctcgccgttg
    13381 tagcttttaa ccgtgcgcac catgcgaccc aagccccgtt tcagacggtc tgttatcgct
    13441 tgctcaatat cagtgatcac gcccgaacac cttattttta ccgtttacaa acacaatacc
    13501 gtcttcgccc gcggccactt cttcgccgct atcatccacg cccagctgca cttcgccttt
    13561 ggccatagca cgcagcaggt cgagcacgtc gattttgtag cggttgcgga tttcgtcggt
    13621 aatcaacacg ccctgagccg ccgtcagacg gtagcgggca atgtcgcagc aaaggcgcac
    13681 caagatgggc ggcagatcct caaaaggtcg tctgaaccgc cccagatacg cgtcgatttc
    13741 ggcagtggcg tccaccagcg cggtttgtgc gacctcgcgg tcaatcagcc cctcgttgtt
    13801 gcggtcggtg agctgcaaga cttccagctc accgaaacgc gcaaccatat cctcaaccgt
    13861 cgcgtatgcc attactcgac cgccttgcgt tgcagcatag gctcggcgca gattgccttc
    13921 cacaccgctt cgccgacttc ggcgcgcttc acttcgcgcc agccgccgtc aaacagcagg
    13981 ccgccgcgcc aaaattcttt gccgtctgcg ccggtactga cgagcatcac atcgcggctg
    14041 tccgccaaag cgtcggcggc acgttgcgta tgctgcactt tgagttcggc aagttcggcg
    14101 gacagtgcct ttttgtcgtc ttcggctttt tccaaggctg tggtcagcat ttcgacatcg
    14161 tttcgggcgg cggcaagctc tgcctgcacg gcgtccaatt cggctttgat gtcttcaaac
    14221 gacggggcgg cggtttcggc ggtttctggt ttgttgttgg tttttgccat gatgactcct
//
LOCUS       DAWHRA010000043        13928 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWHRA010000043 DAWHRA010000000
VERSION     DAWHRA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.043"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(66..1859)
                     /locus_tag="WIB30_000952"
     CDS             complement(66..1859)
                     /locus_tag="WIB30_000952"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ3797036.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKLKAQGSGTI"
     gene            2104..3213
                     /gene="proB"
                     /locus_tag="WIB30_000953"
     CDS             2104..3213
                     /gene="proB"
                     /locus_tag="WIB30_000953"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ3797037.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3226..4488
                     /locus_tag="WIB30_000954"
     CDS             3226..4488
                     /locus_tag="WIB30_000954"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ3797038.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDEKRIGAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(4713..7757)
                     /locus_tag="WIB30_000955"
     CDS             complement(4713..7757)
                     /locus_tag="WIB30_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ3797039.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAAPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSHMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDA
                     WVVEPPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPSGFEQAQRSRIAETDHLADDVLN
                     GGWQEETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAICPFEDVPSERPSCRVSDT
                     EADEGAFQSEETDAVYEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDNPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            7922..8602
                     /locus_tag="WIB30_000956"
     CDS             7922..8602
                     /locus_tag="WIB30_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ3797040.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            8631..8990
                     /gene="crcB"
                     /locus_tag="WIB30_000957"
     CDS             8631..8990
                     /gene="crcB"
                     /locus_tag="WIB30_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ3797041.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASLSPATGNLFANWIGA
                     FLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQLNRPASALSNIFLHTV
                     GSLLLTWLGLKIGAAVK"
     gene            complement(9657..10193)
                     /locus_tag="WIB30_000958"
     CDS             complement(9657..10193)
                     /locus_tag="WIB30_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ3797042.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(10227..10583)
                     /gene="folB"
                     /locus_tag="WIB30_000959"
     CDS             complement(10227..10583)
                     /gene="folB"
                     /locus_tag="WIB30_000959"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ3797043.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            10639..11241
                     /gene="plsY"
                     /locus_tag="WIB30_000960"
     CDS             10639..11241
                     /gene="plsY"
                     /locus_tag="WIB30_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ3797044.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPVAASFF
                     MPHVSWVWATVAIALLVLFRHKSNIVKLLEGRESKIGGSR"
     gene            11318..12163
                     /locus_tag="WIB30_000961"
     CDS             11318..12163
                     /locus_tag="WIB30_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ3797045.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKELISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            12409..13383
                     /locus_tag="WIB30_000962"
     CDS             12409..13383
                     /locus_tag="WIB30_000962"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ3797046.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPDGELKTESFLQAGRHQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     TGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(13526..13744)
                     /locus_tag="WIB30_000963"
     CDS             complement(13526..13744)
                     /locus_tag="WIB30_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ3797047.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         3199 a         4009 c         3442 g         3278 t
ORIGIN      
        1 actatatcgc cgcaatgccg tctgaagcct ttttatgctt cagacggcat cgtctgtttt
       61 cacgctcaaa tcgtaccgct gccttgcgct ttcagtttgg cggcactaaa ttccagtttg
      121 ctcaaggcgg aaaggtaggc tttggcggta gcgaccaaaa cgtcggtatc cgcgccctga
      181 ccgttgacaa cgcggttgcc gcgcgccaga cggacgctgg tttcgccctg gctttccgta
      241 ccttgcgtga cggcgttgac ggaataaatc tgcaaagccg cgccgctttg cgccacgctt
      301 tcaatcgctt tgaaaatcgc atccacgggg ccggagcctg tggcggaagc gcgtttttct
      361 tcgcctttga tgctgaacac gatgtcggcg cgcggctctt cgccggtttc ggtgctgatt
      421 ttttgggaga tgaatttgta gctctcggca ttcatgctgc ccatttcgtc ggataccagt
      481 gcgtgcaggt cttcatcgaa gatttcgcgt tttttgtcgg cgagttcttt gaagcgggca
      541 aacgcggcgt tcagtgcctc ttcgctttcc aactcgatgc ccaaatccgc cagcttggtt
      601 ttgaaggcgt tgcggccgga caatttgccc aagctcaaac ggtttgttgc ccagccgacc
      661 gattcggcgg acataatctc gtaagtttcg cggtgtttca gcaccccatc ctgatggatg
      721 cccgattcat gcgaaaaggc attggcaccg acaatggctt tattgggctg cacgggatag
      781 cccgtaatag tggacaccag tttggacgaa ggcacgattt gcgtggtatc gatgccggtt
      841 tccaagccga acaagtcgtg gcgcactttc aacgccatca cgatttcttc aaccgaagca
      901 ttgcctgcac gttcgcccaa gccgttgaca gtacattcca cctgacgcgc gccgcctttt
      961 aatgcggcaa gcgaattggc aaccgccaag cccaaatcgt tgtggcagtg tgccgaccaa
     1021 acgactttgc cgccgttggg cgttttggca atcagttcgc ggaaaaattc ttcggtttta
     1081 tacgggatgg aatagccgac ggtatcggga atattgatgg tggtcgcgcc cgcttcaatc
     1141 accgcgccgc agatttcggc aaggaaatcg atttccgaac gcaacgcgtc ttcgcaggaa
     1201 aattccacat cgtcggtgta ttcacgagcg attttcaccg ctttgaccgc cgcctcaatc
     1261 acctgcttcg gcttcatctt caatttgtac tccatatgga tggggctggt ggcgatgaag
     1321 gtgtggatgc gttttttcgg cgcgggcgca acggcctcac ccgcctgacg gatgtcccgc
     1381 tcgatggcgc gggacaatga acagaccgtt gatttggtaa tggttttcgc aatcgcattg
     1441 accgcctcga aatcgcccgg gctggcagcg gcaaaacccg cttcgatgat gtccacaccc
     1501 aatttttcca gctggcgggc gacgcggatt ttttcctctt tggtcatagc ggcgccgggc
     1561 gactgctcgc cgtcgcgcag ggtggtgtcg aaaatgataa cgcggttggc ctgtgtcatt
     1621 tctgttctct ccagagattc gtttttttgt aaaaaagcat tcaaatccag cggcctgcct
     1681 tgcgcttccg ccaacgcggt cagcttttgc agttgcgcca gaggcagcga gccgcgcgtg
     1741 cgccatttat agatagcggc ggtcgtcgcg gcattttcgg gatcgtgctg tttcaacgct
     1801 tcggcaagcg cgttcacgcc gccgaaataa gcaaccaagc ggtctatatc taattgcatg
     1861 gtagtccttg tctaaaataa atcagcctac agcagaaata tggacaggat tatacgcatt
     1921 ttggacaaaa cggcaatata aatttgaaaa atctcaataa gccgccatag attttattaa
     1981 ttttatacat tttgtccatt ttattgcaga cgcggcgtga ttgttgacaa ttgcggcaga
     2041 acagggcaaa ttcggcggca caaggtggca acgagacagg acaaaccaac aaggacggaa
     2101 acaatgaaat acaaaagaat cgtatttaaa gtcggcacat cttcgattac ccattcggac
     2161 ggcagtctct cgcgcggcaa aatccaaacc atcacctgcc agcttgccgc attgcatcat
     2221 gcgggacacg agctggtctt ggtgtcttcc ggcgcggttg cggcagggtt cggtgcgctg
     2281 ggtttcaaaa aacgtccggt caaaatcgcc gacaaacagg cttccgccgc cgtcgggcag
     2341 gggctgctga tggaagaata tacggcaaac ctgtcttcag acggcatcgt gtccgcgcaa
     2401 atcctgctca gccgcgccga ctttgccgac aaacgccgct accaaaatgc cggcggcgca
     2461 ctttccgtgc tgctgcaacg ccgcgccgtc cccatcatca atgaaaacga tacggtttcg
     2521 gttgaggaat tgaaaatcgg cgacaacgac acattgagtg cgcaagtggc ggcgatgata
     2581 caggcagacc tcttggtgct gctgaccgac atagacggtc tttacaccgg caacccgaac
     2641 agcaatcccg atgccgtacg gctggacaaa atcgaacaca tcaaccatga aatcatcgaa
     2701 atggcgggcg gctcgggttc ggcaaacggc acaggcggta tgctgactaa aatcaaagcg
     2761 gcgacgattg cgaccgagtc cggcgtaccg gtctatatct gttcctcgct caaacccgat
     2821 gcacttgccg aagcggcaga taatcaggcg gacggctcgt ttttcgtccc ccgtgccaaa
     2881 ggtttgcgga cgcagaagca atggctggcg ttctattccg aaagcagggg cggcgtttat
     2941 gtggacgaag gtgcggaaca cgctttgtcc gaacagggaa aaagcctgct gatgtcgggc
     3001 attgccggaa tcgaagggca tttttcccgt atggacaccg taaccgtgta cagcaaggca
     3061 accaaacagc ccctaggcaa agggcgcgtc ctgttcggtt ctgccgccgc cgaagacctg
     3121 ctcaaatcgc gtaaggcgaa aggcgtgttc atccatcggg acgactggat ttccatcacg
     3181 cccgaaatac gcctgcttct gaccgaattt tagaaaagga aacccatgtc aaacacacaa
     3241 aaacagcttg cccttgccaa agcggcaaaa aaatccgtca acacggcgga tacggaagaa
     3301 aaaaaccgcg cgctgcttgc tatggcggac agcttggaag cggcggcggc agatattttg
     3361 gcggcaaacc gtcaagactt ggaagccgcg gcaggcaaca ttcccgaaag catgaccgac
     3421 cgccttttgt tggacgaaaa acgcattggc gcgatggcgg acggcatcag ggcggttgcc
     3481 gcgctgcccg accccgtggg cgaaatactg gaaacctcga ctttgcccaa cggcttggaa
     3541 atcgtcaaaa agcgcgtggc gatgggcgtt atcggcatta tttacgaaag ccgcccgaac
     3601 gttacttccg atgcggcggc tttggcactg aaaagcggca gcgcggtcgt actccgcagc
     3661 ggcaaagatg cattccaatc cgcacgcgcc atcgttgccg ccctgaaaac ggggttggca
     3721 caaacccgca tcgaccccga cgcgctccag ttgattgaag acaccgggcg cgaaagcagt
     3781 tacgaaatga tgagggcgaa agattatcta gatctgctga ttccgcgcgg cggggcgggg
     3841 ctgatacggg cggtggttga gaatgccgtc gtgccggtca ttgaaacggg aacgggcatt
     3901 gtccacattt atatcgacaa ggacgctgat tgggataagg cactccgtat cgtgtacaac
     3961 gccaaaacca gccgtccgtc cgtgtgcaac tcgatggaag tgctgctggt gcatgaagac
     4021 attgctgccg acttcctgcc caagctcgaa cgactgttgg ttcgcgaccg catagaagcc
     4081 ggactgccgc ccgtccgctt ccgtttggat ccgcaggcgg cgcggcatat cggcggcgaa
     4141 gcggcgggtg cagacgattt cgataccgag tttttagact acatcctcgc cgtgaaaacc
     4201 gtcgcatcgg tcgaagaggc ggtcgggcac atcgaagccc acggcacaca ccattcagac
     4261 ggcatcgtta ccgaaaaccg ccacgctgcg gactatttca cgacccatat cgattctgcc
     4321 gccgtgtatg tcaacgcgtc cacgcgcttt accgacggcg gcgaattcgg cttgggttgc
     4381 gaaatgggca tctccacgca aaaactccac gcccggggcc cgatgggttt gaaagagctg
     4441 acgagttaca aatacatcgt acaaggcacg ggacaggtca gggaataatc cgaattcaaa
     4501 tgtctgatat agtggattaa ctttaaaccg gtacggcgtt gcctcgcctt agctcaaaga
     4561 gaacgattct ctaaggtgct gaggcaccaa gtgaatcggt tccgtactat ttgtactgtc
     4621 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataaaaatgc cgtctgaaac
     4681 agtcttcaga cggcatttcc atttgcaaaa aatcaagcat tgtccaaggg gacgagaatc
     4741 gtacggttgc cgttgtgttc cggtgcggac acaatgcctt ccgcctccat ctggtcaatc
     4801 agacgcgcgg cgcggttgta gccgatacgc aaggcgcgct gtacgcccga aatgctggct
     4861 ttgcgcgttt tcaggacaac ggatacggcc tcgtcgtaca tcggatcggt ttcgtcgtcg
     4921 ccgctgcgcc cgatgccggg cagctcttcg ctgccgccgc cgctcaaaat atcgtcaaca
     4981 tagtccggtt cgccaaactg cttcagatat tcgaccacgc ggtgcacctc ttcatccgag
     5041 gcaaacgcgc cgtgaacgcg ctgcggatag gccgtacccg gcggcaggaa cagcatatcg
     5101 ccctgaccga gcaggttttc cgcgcccatt tggtcgagaa tcgtgcggct gtcgattttg
     5161 ctggatactt gaaacgcgat acgcgtcggg atgtttgcct taatcagacc cgtgatgaca
     5221 tcgacgctgg ggcgttgcgt ggcaagaatc aaatggatgc ctgccgcgcg ggctttttgg
     5281 gcgaggcggg caatcagttc ttcgattttc ttgcctgccg tcatcatcag gtcggcaaac
     5341 tcatcaacca cgaccacgat aaacggcaat ttttccaaag gttcgggatt gtcgggcgtg
     5401 aggctgaacg ggttgccgat tttctccccc cttgctgcgg cttcggcgat tttttgattg
     5461 aaacccgcaa gattgcgcac gcccataaag ctcatcaggc ggtagcgttt ttccatttcg
     5521 ttaacacacc agttcagcgc gtttgccgcc agcttcatat cggtaacgac aggggcgagc
     5581 aggtgcggga tgccttcgta aatgctcaat tccagcattt tcggatcgat cataatcata
     5641 cgcacgtctt ccggcgcggc tttgaaaagc atagacagaa tcatcgcgtt gacacccacc
     5701 gatttgcccg aaccggtcgt accggcaacc aataaatgcg gtgctttgcc caagtcggtt
     5761 acgacgggct gtccggtgat gtcctgaccg agcgcgagcg tcagcttgga tttggattcg
     5821 gcaaactcgg gcgaattgaa gatttcgctc aggcgtatca tttggcgttt cgggttcgga
     5881 agttccaaac ccatacaggt tttgccgggg atggtttcga caacgcggat ggaagccacg
     5941 ccgagcgaac gcgccaagtc tttttccaga tttagaacgg aattgccgcg cacgccgaca
     6001 tcgggttcga tttcataacg cgtaatcacg gggccggaat aagaatcgac aaccttgacc
     6061 ttgactttga actccgccaa tttttcttcg atggtgatgc tgttttccaa cagttcttct
     6121 tcggtttgcg tcgcctcggg attgaacagc ggaggcagaa gcaggtcggt tgtcggcagg
     6181 tgttcgtata ccgcatcggt ttcttcagat tggaacgccc cttcatccgc ttccgtatcc
     6241 gatacccggc aggacgggcg ttcagacggc acatcttcaa acggacaaat cgcctgactg
     6301 tcatgcccga acgcttcggt ttccgacaga tattgcccgt ttgaccgctc tgccgcacct
     6361 tcactgccgt catctgcaat agcggcggtt tcctcctgcc aacctccatt caaaacatca
     6421 tcagcaagat ggtcggtttc agcaatgcgg ctgcgttgcg cctgctcgaa tcctgacggc
     6481 ggttcatagg tacggttgta gatttccgat acgggaggcg gcggcggaat atcgattgcg
     6541 ggcatgggaa ctttcggcac ttcgggtggt tcgaccaccc aagcatcggg cgcagcaggt
     6601 tcttcaatat agatatcggc agcttcatca taaaccggac cgccgtatcc cacagacgaa
     6661 acggtttccg tgaaaacatt ttcggtttca gacggcattt cgacatcccg tttcccgaca
     6721 accgtccgac tttccggaat caggcgcgaa atccgcgcct ccgcagaaac cgttgccttg
     6781 ttcggttctt tcaaattgac ggaaacgcgg cggcaggcat cgcggacggc tgtgccgtct
     6841 gaaacggact gcccttggga aacatccggg gattttgcct ccgcctgccc tttcccctgc
     6901 cctgcatgac ggcggaacgg cgcattccgt gcattttcgg ctttgactgc agaaaaggca
     6961 gacggatgct gcttctcaaa atacggctca aatccgtaat ccgcagactc ggaaaacgct
     7021 tctttgtccg catcgaacat atgggaatac gtgcgttgaa gcacagggtc gtccaaaccg
     7081 ataatctgca gcccttccat cggggtatcg gacacgcgga ctttgggggc cgctgtttcg
     7141 tttttctcaa atgcatcgat atagcgtttt ttcgtttccc ttaaagccgc gctgtttaag
     7201 gcacgcgttg cttcttccaa agtgatttct ttaaaaaccg gacggacggg cgaaatttcg
     7261 ctttcagacg gcatcaaccc ttcagcaata ctccggtcga atgggatgcg gcggttgtcg
     7321 attacggcag ttgcaatgtc ttccgtatcg gcagcctctt cctccgcagc ttctgctgct
     7381 tccgtttccg cttcttccgt cccgtttcca ctgtctgaat atccgtcgga tgcgttcgga
     7441 acatccgcac ggtttgcaga agcaggcgga acaggcctgt ccggttcgga ttcgtgcgcc
     7501 aaatagtttc ggcagaaacg gacaacaccg acaaacagcc aatacactgc cgttttgacg
     7561 gcatggaaaa gcatcagggc aagttcggga aaatcgggca taccgtcttt gatttcaggc
     7621 agctccgcct gtttttcccc tttcttttcc tgccacgcag aaacctcgcg catccactcg
     7681 cgttcggatt gtgcgcggac aaaaaacaag ccggcaagcg caagcaacaa aataacgatc
     7741 aaaactatcc aaaacatatg ccgtttcccg caagacaaac gattaaacgg gtttatttta
     7801 acgtgtttcc atcccaaaaa aaacaaccgg tcggcaattc tgccgaaact gcttcggctt
     7861 tcggtttgcc cccgccgaca gtataatagc cgtcctttcc cttaatcttt gcggcattgc
     7921 catgattttc caaacagtct ggttttcaga tatggtactg tcggtttcgt ggattgtcct
     7981 tatcctgata ctggcagctt ccgcgccgtc cgcattccgc tcgcttgccc gctaccgttc
     8041 tgcccttccc ctgtgtaccg taattttttc cgcagcatgg tgtctgaacg cctctgccgg
     8101 cggcggacag cttgcccaga tgaactacca cctgctcgcc gtcaatctgg ttgcactgat
     8161 ggtgggcact tccgccgcct tttggcttgc cgcgctgctg atgctgccct actgcctgct
     8221 gtttgccggt tctgccggcg catatccgcc gaacgcttta gtgctgattc tgcccgcgct
     8281 tgccgtcaac cgcctgtcgc gtatgctggt caaccgcctg ccgcccaata ttttcatctt
     8341 tattttcgtc aacggctttc tcgcttccgc cgccggcatt ctgctgaccg ggctggtgct
     8401 gaccggtatt ttggatgccg caaacgcttt tccgtccgaa atattgtgga cgaccgccct
     8461 gcccgtcttt attctgctgg cgtgggcgga agcttttctc agcggcattt caactgccat
     8521 atttgttgca ctgaagcccc attggatcaa cactttcgac gacaaccgct atctgaagtc
     8581 cgaccgcggc atatggcggt agccttcaga cggcatagac aggcaaaacc atgctttcca
     8641 acatcatccc cctttccatc ggtgcggcac tcggtgcaac ggcgcgttgg ctgctcaacc
     8701 tcgccgttcc cgcatcgttg tctcccgcca caggcaacct gtttgccaac tggataggcg
     8761 cttttcttat cggaatcttt gccgaaaccg tcaaccaccc gcaatggaag ctgctgctga
     8821 ttaccggttt tctcggcagc ctgaccacgc tttccggatt ttcactggaa accgtaaccc
     8881 tgctccaatt gaatcgtccc gcttcagcac tttccaatat tttcctgcat acggtgggtt
     8941 cgctgctgct gacttggctc gggctgaaga taggggcggc agtcaaataa ccgcaacaaa
     9001 ttgcctccct ttgttttgac ataatctata aattagcttc gcttgtttaa atactggctg
     9061 tgccaattat agtagattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
     9121 atagtacgga accgattcac ttggtacttc agcaccttag agaatcgttc tctttgagct
     9181 aaggcgaggc aacgccgtac tggtttttgt taatccacta taccgttctc aaattcaaat
     9241 tgggctttta ggtctataac aaagaaaaag ccttggtagg ataaacctac caaggctttt
     9301 tcttaaaaaa acgctctatt accgtgccta aaaatgcaaa aaaagtaacc atcataaccc
     9361 ttttgccaaa attaatatca tctattaatt catcatgcca tgcattaata gatggtatat
     9421 aagaaagagc caataaacta ataaaccaat aacaaaaatt agtaacaaag atattcatta
     9481 ttaaccattt taaaaattat catgttcgtt tgggtaatta tcataccctt ttccctctta
     9541 ttactaaagc acgaatgact ttgaaggacg cgcccagaga caagccgaaa aggcatatct
     9601 catccgccaa acaaaatgcc cgccgatcta ctccggcggg caattccaac atcctgtcaa
     9661 tctttcatca gccagtattg taaaccgatt aatgttttac catctttaat ttcatcgttt
     9721 gccaatgcct gacggacttc ttctttcgac atcaatacgg tttccgtaat ctcgtcttcg
     9781 tcattggcaa gcgtactgcc caaacgcacg ccttccgctt cgaacagata cattttttcg
     9841 ttgcaaaaac cgaccgccgt ataaaaactg taaagcaggc gcacgctgtc ggcggtataa
     9901 ggtgtttcct ccgccaattc tcgcagcgca cacgctgcca tatcctcgcc cgccacatcc
     9961 agcttgcccg caggaagttc caatgtcgcc tgatttgccg cataacgcca ctgccgcacc
    10021 aaaaccactt tcccctcgtc cgtaaccgcc aacacgcatg ccgcacccgg atggcggatg
    10081 acgatacgct gcccttcatt gccgttgggc aacctgacct tatccctgct gatactgacg
    10141 aaaccgccct cgtaaatggt ttcgccgcct aattttactt ccctcaaatc cacttcatat
    10201 ctcctttccg tttcctattc tgccgttcaa tcttcacgct tgccgcgctc gatttccacg
    10261 ccaacctcgc gcacgccttc caaaataccc ggcttgacga ttttcacgcg cacccacacc
    10321 gcgccgaaat atcccaaaac caaatcggca atatattccg ccaacgcttc caaaagcagg
    10381 aaatcctgtt ctttcagatg tcggcgcagc gtttcgcata cctcggcata atgcaccgta
    10441 ttggcaatat cgtcgtccga acccgctttc tcgggaacac cgatgtccaa atcgacaatc
    10501 agggtctgca accgttcgcg ttcccagccg tacacgccga taagcgtatc cgccttgatg
    10561 ccgtgcaaaa agattttatc cataatccgc cccgtttttt gctaaaataa ccgcaccatt
    10621 ctaagtaaag taccgcaaat gttcaacata ccggctgttg ctgtttccta tctgatcggc
    10681 tcactttctt tcgccgtcat cgtgtccaag tattacggca tggacgaccc gcgcacctac
    10741 ggatcgggca atccgggggc aaccaatgtt ttacgcagcg gcaaaaaaaa ggcggccgcg
    10801 ctgacgctct tgggcgatgc cgccaaaggt ttagttgccg ttttgcttgc acgcgtgctt
    10861 caagaaccgc tcggtttatc cgacagcgca atcgcggccg tcgcactcgc cgcgctggtc
    10921 gggcatatgt ggccggtgtt tttcggattt aaaggcggca aaggcgtggc aacggcattg
    10981 ggcgtgcttc tggcactctc tcccgcaact gccttggtct gcgcgttgat ttggcttgtt
    11041 atggcattcg gcttcaaggt gtcctccctt gccgcattaa ccgccacaat cgccgcaccg
    11101 gtcgccgcat ccttctttat gccgcacgtc tcgtgggttt gggcgaccgt cgccattgct
    11161 ttgctggtgt tgttccgcca caaaagtaat atcgtcaagc tgctcgaagg cagagaaagc
    11221 aaaatcggcg gcagccgctg atttcccatc catccctgtt tatgccgtct gaaccccgct
    11281 tcagacggca tccgaatatc ccgccgtttt accgactatg ctcagttaca gacacgcatt
    11341 ccacgccggc aaccacgccg atatgctcaa acatttcacc ttgtttttag tgctgcaata
    11401 tttcaaccgc aaagacaagc cctactggta catcgacacg cacggcggcg cgggtgtgta
    11461 caatctcgaa ggcagcgagg cgcagaaagt cggcgaatac cgacaaggca tcgccctgct
    11521 ccgacaggcg caaaacctgc ctgccgaact ctctgacttt gccgcgcaca tacaaaaaat
    11581 cctgccctcg cccgaacttt actgcggttc tccgtggctg gcgcaatcgc tgacccgcgt
    11641 cggcgacaaa ttacgcctgt ttgagctgca ccccaccgac tttgtccatc tgcaaaacaa
    11701 tatgggcgaa gcaggattgg gcaaacgcgg acaagtgttg cgcgaagacg gttacaaaga
    11761 gctgatttcc ctgctgccgc ccccgccgcg ccgcgccacc gtcctcatcg acccgcccta
    11821 tgaggaaaaa caggactacc ggcgtgtaac ggaaacgctg aaagccgctt taaaacggtt
    11881 tgagtccggc tgttatctca tttggtatcc ctgcctcagc cgcgaagaaa gccgcaagct
    11941 gcccgaagaa ttgaaaaaac tcctgcccga caactacctt cacgccgaac ttcacgtcca
    12001 cgcgccgaaa gccgacggtt tcggtatgca cggcagcgga atgttcgtca tcaatccgcc
    12061 ctatctgttg gcggaacaat tggcggcaaa cctccccgcc ctgacgcggc tgctggcgca
    12121 agacgaaggc gcgcgctacc ttttggacag caaaatccgc tgaccccgat gccgtctgaa
    12181 acccttcaga caacatctcc cgtcaaataa acaaaaccgg acgatatacc ggcagtcggc
    12241 acttttaaga ataaatatcc cctcatccgc ctgccgcgtt ccgacttgcc ccgcgccgtt
    12301 tttttccact acaatcccac gcgatatctg gcggaacgca ccaaaggaac gccccgcagc
    12361 aatctgccgc ctttcagacg gcacaaacct ttccgaaacg agcaaactat ggacacactg
    12421 acccgattcc tccccgaaca cctgcaacaa aaccagctgc ccgaagcact cggcggcgtt
    12481 ttactgtccg ttgtttccgc ctgcaccgaa atcaacgcca aagtccgcct cggcgcactg
    12541 gccggcgtat tgggtatggc gggcacaggc aatattcagg gcgaagacca gaaaaaactg
    12601 gatgttatcg ccaacaacat tatgattgac acactcaaag ccaaccctgc cgttgccggt
    12661 ttggcaagcg aggaagaaga cactttcgta agtgccggtg aaaacggacg ctatctcgtc
    12721 ctattcgacc ctttagacgg atcgtccaat attgatgtca acatttccgt cggtacgatt
    12781 ttctccatcc ttgaaaaacc cgatggcgaa ttaaaaaccg agtccttctt gcaagccggc
    12841 cggcaccagc ttgccgcagg ctatgtcctc tacgggcctc aaacccagct cgtattcaca
    12901 ttcggacacg gcgtatatat gttcacgctt aatgccgaaa acgaatttgt gctgaccaaa
    12961 gaaaacccga aagtacccga aagtaccaaa gaatttgcca tcaatatgtc caaccgccgc
    13021 cactggctgc cccccgtcca acaatacatc gacgagctct tgacgggcga aaccggcacg
    13081 cgcggcaaaa actacaatat gcgctgggta gccagcatgg ttgccgaaat ccaccgcatc
    13141 ctgatgcgcg gcggcgtgtt catgtatccg caagacaaac gcgaccccgc caagcccggc
    13201 aaactgcgcc tgatgtacga agccaaccct atgagcctga tactcgaaca ggcgggcgca
    13261 tcggcaagca acgcatatca agccatgctc gacatacagc ccgaaaacct gcaccaacgc
    13321 gtcgccgtct tcatgggcag cagcgaagaa gtcgcctatc tcgaccgcct gcacgcaaaa
    13381 tagacagaca gccgttcaca caagtacacg gcggcagcaa tccgataccg ccgccgttat
    13441 ttcaaacagc acaagccttc cggtcaaatg acaaaaatgc cgtctgaaca catttcagac
    13501 ggcatttcaa aaactcaaac ccgaattatt ttcccgctgc tttttgcagg atggcgtaca
    13561 actcgtcttt cagtttcaat ttggcttttt tcagctcatc gatggtttcc gcaccgctgg
    13621 taaccggatt gttgaccaga ccggtaattt tatcgtccag ctcgttgtgt tcgtcgaaca
    13681 gacgggcgaa gcgggaattt tcctgtttca atttggaaat caaatcacgg tattctggga
    13741 acatatcgct ttcctctctt ggtttacggt taaaaagtca aattgaatct ttacattaat
    13801 tataacaaag ataatatttt tatccagtgt tttatcggat acggcaaaat tttcttttaa
    13861 caccgccgtc cggttgataa aaacgttgac aatatcacgc tttacgcata aattttcagc
    13921 ctttccta
//
LOCUS       DAWHRA010000044        13803 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWHRA010000044 DAWHRA010000000
VERSION     DAWHRA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13803
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.044"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..334
                     /locus_tag="WIB30_000964"
     CDS             <1..334
                     /locus_tag="WIB30_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797048.1"
                     /translation="ASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAISSG
                     MKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYKSPA
                     AWNEGAAK"
     gene            complement(396..965)
                     /locus_tag="WIB30_000965"
     CDS             complement(396..965)
                     /locus_tag="WIB30_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ3797049.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            1123..1983
                     /locus_tag="WIB30_000966"
     CDS             1123..1983
                     /locus_tag="WIB30_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ3797050.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            2118..2456
                     /locus_tag="WIB30_000967"
     CDS             2118..2456
                     /locus_tag="WIB30_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797051.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGSC"
     gene            2824..3177
                     /locus_tag="WIB30_000968"
     CDS             2824..3177
                     /locus_tag="WIB30_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ3797052.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            3167..4033
                     /gene="atpB"
                     /locus_tag="WIB30_000969"
     CDS             3167..4033
                     /gene="atpB"
                     /locus_tag="WIB30_000969"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ3797053.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            4090..4326
                     /gene="atpE"
                     /locus_tag="WIB30_000970"
     CDS             4090..4326
                     /gene="atpE"
                     /locus_tag="WIB30_000970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ3797054.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            4397..4867
                     /locus_tag="WIB30_000971"
     CDS             4397..4867
                     /locus_tag="WIB30_000971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ3797055.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            4872..5405
                     /locus_tag="WIB30_000972"
     CDS             4872..5405
                     /locus_tag="WIB30_000972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ3797056.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            5416..6963
                     /locus_tag="WIB30_000973"
     CDS             5416..6963
                     /locus_tag="WIB30_000973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ3797057.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            6988..7863
                     /gene="atpG"
                     /locus_tag="WIB30_000974"
     CDS             6988..7863
                     /gene="atpG"
                     /locus_tag="WIB30_000974"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ3797058.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            7901..9298
                     /gene="atpD"
                     /locus_tag="WIB30_000975"
     CDS             7901..9298
                     /gene="atpD"
                     /locus_tag="WIB30_000975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ3797059.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            9309..9731
                     /locus_tag="WIB30_000976"
     CDS             9309..9731
                     /locus_tag="WIB30_000976"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ3797060.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            10055..10960
                     /gene="glyQ"
                     /locus_tag="WIB30_000977"
     CDS             10055..10960
                     /gene="glyQ"
                     /locus_tag="WIB30_000977"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ3797061.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            11036..11446
                     /locus_tag="WIB30_000978"
     CDS             11036..11446
                     /locus_tag="WIB30_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ3797062.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQALIFGVRGSLHQAA
                     DDAEAFDRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            11467..13530
                     /locus_tag="WIB30_000979"
     CDS             11467..13530
                     /locus_tag="WIB30_000979"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ3797063.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAALGEVDESLLQQDEEKALYAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            13547..>13803
                     /locus_tag="WIB30_000980"
     CDS             13547..>13803
                     /locus_tag="WIB30_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ3797064.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIAQRFQEQDGRIRILAQPRNSG"
BASE COUNT         3449 a         3549 c         3590 g         3215 t
ORIGIN      
        1 cgcatccaaa gcggacatcg ccgagaacct gaaaaacatc aaaatcgtcg agcttgaagc
       61 cgcgcaactg ccgcgtagcc gcgccgacgt ggattttgcc gtcgtcaacg gcaactacgc
      121 cataagcagc ggcatgaagc tgaccgaagc cctgttccaa gaaccgagct ttgcctatgt
      181 caactggtct gccgtcaaaa ccgccgacaa agacagccaa tggcttaaag acgtaaccga
      241 ggcctataac tccgacgcgt tcaaagccta cgcgcacaaa cgcttcgagg gctacaaatc
      301 ccctgccgca tggaatgaag gcgcagccaa ataaggcagt cgtataaaat gatgccgtct
      361 gaaccgtatc cgtgttcaga cggcattttt gtcctttaat ccgccattcc ctgccattcc
      421 gccgaatccg gcgtatcgat tccgaacagc gacaaagcgt gtgcgacact gtgcgccact
      481 atgtcgtccg ccgtctgcgg tttgcggtac atcgcaggaa cagggggaaa caccacgccg
      541 cccatttccg ttacccgctt catattgtcc aaatgggcaa ggttcagcgg cgtttcgcgc
      601 accatcagca ccagccgccg cctttccttc aaaaccacat ccgccgcacg cgtcaagagg
      661 ttgtcgccga agccgtgcgc gacagaggca agcgtccgca tcgaacaggg ggcgaccagc
      721 atcccgtccg ttttaaacgt accgctggca atgcacgccc cgatattgcc gatcggatgc
      781 acgaagtccg ccaaggcata tacctcatct ctcgcataag ccgtttccga agcgcgcgcc
      841 atctccgcac ctttcgatac cacaaggtgc gtttcgatat cttgcgcgcg taaaagttcc
      901 aaagccttca cgccgtattg gaaaccgctc gccccgctga tgccgattat caaacgccgt
      961 accatcatcc gcctttccca taaaaccgcc tgcaacggca aaccggctat tatagtgaaa
     1021 aaacagaaat ccgataaaca cggatacaaa ttgtcggcaa cacccaatat ccgataaaat
     1081 acccgattta acatcctatc tgaataggca cgggagggcg gtatggcaaa agtaaaaggc
     1141 ggattggggc gcggtttgga ttcgctgctc gccaacggcg cagacaacag cagcggcgac
     1201 cgattgacca cggttgcggt taaagatatc cggcccggcc gctatcaggc gcgtgttcaa
     1261 atcgatgacg aagccttgca ggagctggca gattcgatta aggcgcaagg cgtgatacag
     1321 cccgtcatcg tgcgcgaaca cggactgtcc cgatacgaac tgattgcggg ggagcggcgt
     1381 tggcgtgccg cacagattgc cggcctgacc gaaatccccg ccgttatcaa aaccatcagc
     1441 gacgaaaccg cgttggcaat gggtttgatc gaaaacctcc agcgcgaaaa cctcaacccc
     1501 atcgaagaag cacaaggctt gaaacgcctt gccgacgagt tcggactgac ccacgaaacc
     1561 atcgcccaag ccgtcggtaa aagccgaagc gcgatttcca acagcctgcg ccttttaagc
     1621 ctgcccgaac ccgtgcagga aatgctttac caacgccgcc tcgaaatggg gcacgcccgc
     1681 gcactgctga ccctgcccgt cgtcgaacag ctcgagttgg cgcaaaaggc cgtcaaaaac
     1741 ggctggtcgg tgcgcgaagt cgagcgccgc agccaagccg cccttcaaaa caaacgtccc
     1801 gagcccaaaa aaactgccgc cgccgacatc ggccgcctga atgatttgct gactgaaaaa
     1861 ctgggtgtca acgccgaaat caaaaccgcc aaccataaaa aaggcagaat catcctgcat
     1921 ttcgacacgc ccgaaacctt cgaccatatc ttgaagcagt tgggcataga ttaccgacct
     1981 taattttgcg ggatataccg tctgaaatat agagaatagc tttccagatt gtaagtggga
     2041 aatataattc tattgacatt tttctgcttc acgtaagaat cgttttcctg ttttcatttt
     2101 taattttcga ggaaattatg aacacacgca tcatcgtttc ggctgcgttc gttgcgttgg
     2161 cattagcagg ttgcggctca atcaataatg taaccgtttc cgaccagaaa cttcaggaac
     2221 gtgccgcgtt tgccttgggc gtcagcccaa atgccgtaaa aatcagcaac cgcagcaatg
     2281 aaggcatacg catcaacttt accgcaactg tgggtaagcg cgtgagccaa tgctatgtta
     2341 ccagtgtaat cagcacaatc ggcgttacca cttccgatgc aatttgtttg ggaggcggaa
     2401 cgcacaaagg caaaagtcaa tgcaatgctt tgcttaaagc ggcaggcagt tgctaatcct
     2461 ttattcggaa aaggtcgtct gaaaatattt tcagacgatc ttttatttat tgagcaaatt
     2521 ccccaaactg ccgacaatcc gaccgataaa ctgctacaat ttcgcgttga ttttttatta
     2581 tttccacgta aagcaaacaa atcacgtaag aacattaaaa atgtaaagaa attgcggaca
     2641 aactccttaa tatcctgtaa ttatatgcaa tgtgtgttag aatgacactt gtcgccgtta
     2701 ttcaggcggc gagcgcggcg gattgggata cgttgtttca caaagattaa ctgtcgcttg
     2761 acgtaataag gcttctaaat tatattagcc gcgccccaaa tccgaggcgg aaacattccc
     2821 tttatgaagc agattatcat cctacaatcc gccgtattgt ccatatgcgc cgcagttgcc
     2881 tttgccgtat ggggttttgc cggattcctt tcggcggtcg gtggcggttt gtcctacctg
     2941 cttcccacgt ttgttgcagt tttactttta aaacttttca ggggaaaccc cttcctgcaa
     3001 agcaggatgt tcgtcttcgg agagatttta aaagtagtgc tgtcgctgtt gtccatgctc
     3061 gccgtatttg caatatggca tcaatcgctg gtgttcgccc cgtttctgat ggggctgctc
     3121 ggtgtcagcc atttggtttt tttagtattg ttgagagtga aagattatgg caggtgaaac
     3181 cattaccgct gccgactaca tcaagcacca cttgcagagc ttgaccagtt tgtcggatgt
     3241 tactcagggt cagggactga aaaacattgc tgatttttcg tttattaacc ttgatgccgt
     3301 cttttttgcc gtcctgttgg gcgtaatcgg cagcttcctg ttgtggcgcg gtgcaaaaaa
     3361 agcgacggca ggcgttcccg gacgtttcca ggccgccgtg gaaatcttgt ttgagtttgt
     3421 ggacgatatg tgtaagagca tcattcacaa cgaaaagtct agaaaagccg tcgcgccgtt
     3481 gggtctgacg ctgtttgtct ggattttcct gatgaacgcg atggatatgc tgccggtcga
     3541 tttgctgccg atggtatggc agggcattac cggcaaccat cacgccctgt tgcgcgtcgt
     3601 accgactgcc gatttgaaca ccactttggc actcgctgtc ggcgtgttgc tgatttgtat
     3661 ctattacaac atcaaaatca aaggattggg cggatggttt cacgagttgt tcagcgcgcc
     3721 gttcggtgca aaactcgcac ctgcgaactt cctgctgaat ttggtcgagt ttctttctaa
     3781 aaccgtatcc cacggtatgc ggttgttcgg caatatgtat gcaggcgagt tggtattctt
     3841 gttgatagcc ttgcttggcg gtgcttgggc ggcttccggc agcgtcgaag tcatggatcc
     3901 gattctgttt gtattccaca ttattgccgg tttggcatgg gcgattttcc atattttggt
     3961 gattaccctg caggcgttta ttttcatggc gttggcgttc gtctatatcg gacaggcaca
     4021 tgatgcgcac tgattttcct aagtagtttg tttttgtagt ttaacctttg tttctttaag
     4081 gagtttaaaa tgggtttgat tgctatcgca tgtggtttga tcgttgcatt gggtgcattg
     4141 ggtgcctcta tcggtatcgc aatggtcggt tctaaatatt tggagtcttc tgcccgtcag
     4201 cccgaactga tcggtccgct gcaaaccaaa ctgttcctga ttgccggttt gattgatgcc
     4261 gcattcttga ttggtgtcgc cattgcactg ctgttcgcct tcgttaaccc gtttgcaggt
     4321 gcataatcag gacggattcg tccgttccgt acaggcgcag cctgtttgat taaccccagt
     4381 acgaaggtta agtaacgtga atatcaatgc aacattattc gctcaaatca tcgtcttttt
     4441 cggtttggta tggtttacca tgaaattcgt gtggccaccg attgcaaaag ctttggatga
     4501 gcgtgccgca aaagtcgccg aaggcttggc tgccgccgag cgtggtaaaa gcgatttcga
     4561 gcaggctgaa aaaaaggttg cagaactctt ggcagaaggg cgtaatcagg tttccgaaat
     4621 ggttgccaac gccgaaaaac gtgccgccaa aattgtcgaa gaagccaaag aacaggcttc
     4681 ttccgaggcg gcgcgcattg cagctcaggc aaaggccgat gtggagcagg aattgttccg
     4741 cgcacgcgaa tccctgcgcg agcaggttgc cgtgttggct gtcaaaggtg ccgaatctat
     4801 tttgcgcagc gaagtcgatg cttccaaaca cgcaaaactg ctcgataccc tgaaacagga
     4861 gctgtaatct tatggcagag ttcgcaacga ttgccagacc ttatgcaaag gcattgttcg
     4921 gtctggctca ggaaaaaaac caaattgagt cttggttggg cggactggaa aaacttgcgg
     4981 cggttgttca ggaagggaag gtggcttcat tgattgaccg tcctgaaacg aatgcttcag
     5041 aaaaagcaga tatcctcatc gatttggtcg gtttgaaaga caaggagttg aaaaactttg
     5101 ttatcgtctt ggccgggcag aaacgtttgt cgatattgcc ggaagtatat gctcaatatc
     5161 aagacttgac cttatcattc aaccatatca aatctgccgt catttacagt gcctatccgt
     5221 tgaccgacaa acaggtcggc gagttggcgc aaatgctgaa taagcgtttc gacagcgagc
     5281 tgaaaatctc tgtcgaaatc gaaccggagc tgattggcgg cataaaagtt gaagtgggtg
     5341 atcaggtttt ggatttgtct gtacaaggca aattgagtgc cttgtacacg actatgacga
     5401 attaggagag ttttcatgca gcttaatcct gctgaaatta gcgatttgat taaagccaag
     5461 atcgaaaatc tgtctgtaaa tgccgaagtg cgtactcgtg gtacggtgat ttcggtaact
     5521 gacggtatcg ttcgcatcca tggtttgtca gatgcaatgc aaggtgagat gctcgaattc
     5581 ccgggtaaca ctttcggctt ggcgatgaac ctggagcgcg actccgtcgg cgccgtagtg
     5641 ttgggcgagt acgaacatat taaagaaggc gacacggtta cctgtaccgg tcgtatcttg
     5701 gaagtgccgg tcggacgcga actggtcgga cgcgtcgttg atgcattggg tcggcctatc
     5761 gacggcaaag gcccgattaa tacaactttg accgccccta tcgaaaaaat cgcaccgggc
     5821 gtgattgcgc gcaaatcggt tgaccagccg atgcaaaccg gtctgaaggc gattgactct
     5881 atggttcctg tcggtcgcgg tcagcgtgag ttgattattg gcgaccgtca aaccggtaaa
     5941 acggccgtag cattggatgc catcgtcaac caaaaaggta cgggtgttat ctgtatctat
     6001 gtcgctatcg gtcaaaaagc atcttctatt gccaacgtgg tccgcaaatt ggaagagcat
     6061 ggcgcgatgg aacacacgat tgtggttgct gcaactgcat ctgaagcggc ggcattgcaa
     6121 tatatcgcac cttactccgg ttgtacgatg ggtgaattct tccgcgatcg tggcgaagat
     6181 gccttgattg tttatgacga tttgtccaaa caggctgtgg cttaccgtca aatttccctg
     6241 cttttgcgcc gtccgcccgg ccgcgaagcc tatcctggcg atgtgttcta cctgcactcc
     6301 cgtctgttgg aacgtgcggc acgtgtcaat gaacacgaag tggagaaatt gaccaacggc
     6361 gaagtaaaag gcaaaaccgg ttctctgacc gcgttgccga ttatcgaaac ccaagcgggc
     6421 gacgtatctg ctttcgttcc gactaacgtc atttcgatta ccgacggtca gattttcttg
     6481 gaaaccgacc tcttcaacgc cggtatccgt cctgcaatca atgccggtat ttccgtatcc
     6541 cgcgtaggcg gtgctgcaca aaccaaagtg attaaaaagc tgggtggcgg tatccgtttg
     6601 gcgttggcac aatatcgtga attggcggcg ttctcgcaat ttgcatccga tttggatgaa
     6661 gctacgcgca aacaattgga gcatggcgaa gtcgtaaccg aactgatgaa acagaaacag
     6721 ttcagtacgt tgaatacggc tgaaatggct ttgacccttt gggcaatcaa caacggttcg
     6781 tattctgatg ttccggttgc taaagctctg gcttttgaat ctgaattttt gagctttgtc
     6841 cgtacccaac atccggaagt tttggaagcc gtcaatgctt caggtgcaat gtccgacgag
     6901 agcgagaaaa cgcttgaagc agccatgaaa tccttcaaat cttcttacgc ctatcaggca
     6961 taaggcttga agtgaaagga gtctgaaatg gcagtaggaa aagagattct caccaaaatc
     7021 cgcagtgttc agaataccca aaagatcact aaagcgatgc agatggtgtc aacctctaaa
     7081 atgcggaaga ctcaggaacg gatgcgtttg gcgcgtccgt atgccgaaaa agtgcgtatg
     7141 gtgatgagcc atcttgcgca aaccaatacc gatcatggta ttccgttact ggaatctcat
     7201 cgggaaatca gacgtgtcgg ttttattttg attacgtctg ataagggttt gtgtggcggt
     7261 ttgaacgcca acgtgctgaa aaagtttttg gcacaagttc aagagtatcg gaatcaaggt
     7321 attgaagagg aaatcgtatg ccttggcagt aaaggtctga tggcgtgtca gagcattggt
     7381 ctgaatgtgg ttgccagtgc cgtaaatttg ggcgataccc caaaaatgga aatgctgctc
     7441 ggacctttga cagaactctt ccaacggtat gagaaacatg aaattgacag aatccatctg
     7501 gtgtattcgg gttttgtcaa taccatgcgt caagaaccga gaatggaagt attgctgcct
     7561 atcggtgaga acgtgattgg cgattcagct cccaaatcac cgttcagctg ggaataccgc
     7621 tacgaaccga ctgcacttgc agtgttggaa tatctggttc gccgctattt agagtctgtg
     7681 gtttatcagg cgttgagcga caatatggca tccgaacagg cagcccgcat ggttgccatg
     7741 aaagctgcaa cagacaatgc aggcaatgcc atcaaagagt tgcgtttggt atataacaaa
     7801 tcgcgtcaag ctgcgattac cacggaattg tcagaaattg tagcaggtgc ggcggctgtc
     7861 tgatgccgtc tgaatcctgt acggaaatta ggatacgata atgagccaag gcaaaatcgt
     7921 acaaattatc ggtgcggttg ttgacgtgga atttccacgc gacatgattc cgcgcgttta
     7981 cgacgctttg aaattagacg aaaacggtct gactttggaa gtccaacagc ttttgggcga
     8041 cggcgtagtc cgtaccattg cgatgggcag ctcggacggt ttgaaacgcg gcatgactgt
     8101 gagcaatacc ggtgcgccca ttactgtgcc ggtaggtaaa ggtacgttgg gacgcattgt
     8161 cgatgtattg ggaacgcctg ttgacgaggc aggtccaatc gataccgaca agagccgtgc
     8221 catccaccaa gccgctccta agtttgacga actgtcttcc acaaccgaat tgctcgaaac
     8281 gggcattaaa gtgattgact tgctgtgtcc gtttgccaaa ggcggtaaag taggtctgtt
     8341 cggcggtgcc ggtgtgggta aaaccgtgaa catgatggaa ttgatcaaca acatcgccaa
     8401 agcgcacagc ggcttgtccg tgttcgcagg cgtgggtgag cgtacccgcg aaggtaacga
     8461 cttctaccac gagatgaaag attccaacgt attggataaa gtggcaatgg tttacggtca
     8521 gatgaacgaa cctccgggca accgtttgcg cgtcgcattg accggtttga ccatggcgga
     8581 atacttccgt gacgaaaaag acgaaaacgg caaaggccgc gacgtattgt tcttcgtgga
     8641 caacatctac cgttacactc tggctggtac cgaagtatcc gcattgttgg gccgtatgcc
     8701 gtctgcagtg ggctaccaac cgacattggc agaagaaatg ggtcgtttgc aggagcgtat
     8761 tacctctacc caaaccggtt ctattacttc tatccaagcc gtatatgtac ctgcggacga
     8821 cttgaccgac ccgtctccgg caacaacgtt cgcccacttg gacgcaaccg tcgtattgag
     8881 ccgcgatatt gcctctttgg gtatttaccc ggcagtcgat ccgcttgatt ccacttcgcg
     8941 ccaactggat ccgatggtat tgggtcaaga gcactacgac gtggcgcgcg gcgtacagtc
     9001 caccctgcag aaatacaaag aattgcgcga catcatcgcc attttgggta tggacgaatt
     9061 gtctgatgaa gacaaactga ccgtaatgcg tgcgcgtaaa atccaacgct tcctgtccca
     9121 accgttccac gttgccgaag tgtttacagg ttctccgggc aaatatgtcg ccctgcgcga
     9181 taccattgcc ggcttcaaag ccatcttgaa cggcgaatac gaccatctgc ccgaacaggc
     9241 attctacatg gtcggcagca tcgaagaagc ggttgagaaa gcgaaaacct taaactgagg
     9301 aggtcggcat gagcatcatg caagttgagg tggtcagtag cgagcaaaac atctattcgg
     9361 gcgaagccag ctttgtagtg gttccgaccg ttcaaggtga gcttggtatt tacccgcgac
     9421 acgagccgat tatgagtttg gtgcggccgg gggctttgcg tttgaccgtt ccgggcgagg
     9481 ataaagaggt tttggttgct gtttccggcg gtgttttgga agtacagcct gataaagtaa
     9541 ctgtcttggc ggatgttgcc gtccgcagtg cggagatgga tcaggcacgt gcggaagagg
     9601 cgaaaaaagc cgcagaagcg ggcatttccc aagctaaaga cgataaggct ttggcggagg
     9661 cgcataaagc attggctgct gcgattgcac agctcaaaac tttggactat atccgttcgc
     9721 acaagaaata aacttgtttg gtttggaaaa gcacgaccgt ttggtcgtgc tttttatttt
     9781 tccaagtttt gaaaatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
     9841 cagtacaaat agtacggaac cgattcactt ggtgcttgag caccttagag aatcgttctc
     9901 tttgagctaa ggcgaggcaa cgctgtactg gtttaaattt aatccactat atcatgtatg
     9961 ccgtgttttc aaatcagtct gtctttgaga taaacctatc caaacagcaa tggaaacagt
    10021 aaaataaacc ctttatttta tcgaatcata agccatgctt accttccaac aaatcatctt
    10081 caaactgcaa acattctggg cagacaaagg ctgcaccgtc atccaacctt tcgacatgga
    10141 agtcggcgca ggtacttccc accccgccac ctgcctgcgc gcactcggcc ccgagccttg
    10201 gtttgccgcc tacgtccaac ccagccgccg ccccaaagac ggccgctacg gcgacaaccc
    10261 caaccgcctg caacactatt accaattcca agtcgccctt aaacccgccc ccgccaatat
    10321 ccaagacctc tatctcgact ccctgcgcga attgggcatc gaccccaaag tccacgacat
    10381 ccgctttgtc gaagacgact gggaaaaccc caccctcggt gcatggggtt tgggctggga
    10441 agtctggctc aacggcatgg aagtaaccca gtttacctat ttccaacaag tcggcggcat
    10501 tgactgcacg cctgtactcg gcgaaattac ctacggcatc gaacgcttgg cgatgtacct
    10561 gcaaggcgtg gaaaacgtct atgacctcgt ttgggcaaaa acgccggacg gcaacaccgt
    10621 aagctacggc gacgtgtacc accaaaacga agtcgaacaa tccacctaca acttcgaata
    10681 cagcgatgcc gattggctgc tgcgccagtt caacgactac gaagcgcaag ccaaacgcct
    10741 gcttgccgaa gaaaacgccg ccctcgccct gcctgcctac gagctggtcc tcaaagcggg
    10801 gcatacgttc aacctcttgg acgcgcgcgg cgcgatttcc gtaaccgaac gcgccaccta
    10861 catcgggcgc atccgcgcgt tgagccgcgc cgtggcgcaa aaatatgtcg aaagccgcga
    10921 aaaactgggc tttcccttaa tgaaggcaaa tgccgcctag gggagcgacg atttttacgt
    10981 tcgggcggca cgatatggca gccgtccgga agccattata ttttttagga aaattatgcc
    11041 tgtctatttc cgcaccccga gcaggaaacg cctgcacaaa atgaaccgcc gccagcataa
    11101 aaagctgcat ttgggcgagt ttcaagcatt gattttcggc gttcgaggca gtttgcacca
    11161 agctgcggac gatgctgaag cgttcgaccg ctttatggac gagctgattg cgtatcttga
    11221 ggcaaatggc ttggaaatgg cgggcggggg cgattgcggc gatttttcgt gcctgtttca
    11281 aaaatccggt gcccgaagct tgggcgaaac cgagaaaaaa gccattatct cttggctggc
    11341 aggccgcgaa gatgtcgcca ctttgtacgc caccccgctg gttgatggtt tttacgggga
    11401 tgacggctgg gaaaattaca gcgaaaccta taaacaggca gattaaccaa attcaaagaa
    11461 agccgaatga tgacccaaac ccttttaatc gaactcctta ccgaagaact cccgcccaaa
    11521 gccctgaata atctgggcaa ccattttgcc gccgccgttg ccgaaggctt ggaaaaagcg
    11581 caactggttg acggtgcagc cgaatttacg gcttatgcct cgccgcgccg tttggcggtt
    11641 caagtcaaaa acgtcaaagc cgttcaggcc gaccaaaaaa tcgtgaaaaa aggccctgcc
    11701 gtggcgaatg ccgtaaaaga cggtgcgccg accaaggctt tggaaggttt cgcacgcggt
    11761 gcaggcgcga aaatcgaaga cttgaccatc gtccacgacg gcaagcagga tgtgtacgcc
    11821 tacgaatacg tccaaaccgg caagccgctg ggcggacttt tggaagacat tatcaatcaa
    11881 gtggttaaga aactgccgat tcccaaagtg atgcgttggg gcagcagcac atttaccttc
    11941 gtgcgccctg ttcacgggct gattgtgctg cacggcggcg acatcgtaaa cgtcagcgtt
    12001 ttgggtctgc aaagcagcaa tcaaaccttg ggacaccgct tcttgtccaa cggcgaaatc
    12061 accattgaaa acgccgacag ctacgccgaa caaatgcgcg ggcaaggcaa agtcgtcgct
    12121 tcgtttgccg agcgcaaagc cgcgattcag acggcattgg aagggcaggc gcgcagtctg
    12181 aacgccgccg tcgccgccga tgaagcattg ttggacgaag taaccgcgct ggtcgaatgg
    12241 cctgttgttt tggaagccgg ttttgaggaa cacttcctcg ccgtgccgca agaatgcctg
    12301 attctgacga tgcagcaaaa ccaaaaatac tttccgctgc tcgaccaaaa cggcaagctg
    12361 atgaaccgct tcctgttggt ctccaacctg caaaccgaag atccgtcgca catcatccaa
    12421 ggcaacgaac gcgtcttgcg cgctcgcctg tctgatgccg agttcttcta caagcaagac
    12481 cagaaagcga ctttggaaag ccgcctgccc aagctggcga acgtggttta tcacaacaaa
    12541 atcggctcgc aagccgaacg catcgaacgc ctacaaagca ttgccgccca catcgccaag
    12601 gctttgggcg cggatgccgc cgcagccgaa cgcgccgcgc gtcttgccaa agccgacttg
    12661 gtaaccgaaa tggtcggcga gttccccgaa ctgcaaggta cgatgggcaa atactatgcc
    12721 cgcttggacg gcgaaaccga agaaatcgcc gaagctgtcg agcaacacta ccaaccgcgt
    12781 tttgccggcg acaagctgcc cgaaagcaaa attgccgccg ccgtggcact ggcggacaag
    12841 ctggaaaccc tggtcggaat ttggggcatc ggcctgattc cgaccggcga caaagacccc
    12901 tacgccctgc gccgcgccgc cttgggtatt ttgcggatgc tgatgcagta cggtttggac
    12961 gtgaacgaac tgattcagac ggcattcgac agctttccca aaggtttgct caacgaaaaa
    13021 acgccgtctg aaaccgccga ctttatgcag gcgcgccttg ccgtgttgct gcaaaacgat
    13081 tatccgcaag acatcgttgc cgccgtactc gccaaacagc cgcgccgttt ggacgatttg
    13141 accgccaaac tgcaggctgt cgccgcgttc aaacaactgc ccgaagccgc cgcgctcgcc
    13201 gccgccaaca aacgcgtgca aaacctgctg aaaaaagccg atgccgcgtt gggcgaagtc
    13261 gatgaaagcc tgctgcaaca agatgaagaa aaagccctgt acgccgccgc acaaggctta
    13321 cagccaaaaa ttgccgccgc cgtcgccgaa ggcaatttcc aaaccgcctt gtccgaactg
    13381 gcttccgtca agccgcaagt cgatgccttc tttgacggcg tgatggtaat ggcggaagat
    13441 gccgccgtaa aacaaaaccg cctgaacctg ctgaaccgct tggcagagca aatgaacgcg
    13501 gtagccgaca tcgcgctttt gggcgagtaa ccgttgtaca gtccaaatgc cgtctgaagc
    13561 cttcagacgg catcgtgcct atcgggagaa taaattgcag cctttagtca gcgtattgat
    13621 ttgcgcctac aacgtagaaa aatatttcgc ccaatcatta gccgccgtcg tgaatcaaac
    13681 ttggcgcaac ttggatattt tgattgtcga tgacggctcg acggacggta cgcttgccat
    13741 tgcccagcgt ttccaagaac aggatggtcg catccgcatc ctcgcgcagc cgcgcaattc
    13801 cgg
//
LOCUS       DAWHRA010000045        13765 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWHRA010000045 DAWHRA010000000
VERSION     DAWHRA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13765
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.045"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(63..623)
                     /locus_tag="WIB30_000981"
     CDS             complement(63..623)
                     /locus_tag="WIB30_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ3797065.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WIB30_000982"
     CDS             complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WIB30_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ3797066.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLLITPFEAQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2243..3174
                     /locus_tag="WIB30_000983"
                     /pseudo
     CDS             2243..3174
                     /locus_tag="WIB30_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3430..4068)
                     /locus_tag="WIB30_000984"
     CDS             complement(3430..4068)
                     /locus_tag="WIB30_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ3797067.1"
                     /translation="MSKLTPREILSAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAS
                     HNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4249..6084)
                     /gene="edd"
                     /locus_tag="WIB30_000985"
     CDS             complement(4249..6084)
                     /gene="edd"
                     /locus_tag="WIB30_000985"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ3797068.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKAGNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            6651..8096
                     /gene="zwf"
                     /locus_tag="WIB30_000986"
     CDS             6651..8096
                     /gene="zwf"
                     /locus_tag="WIB30_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ3797069.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            8376..9071
                     /gene="pgl"
                     /locus_tag="WIB30_000987"
     CDS             8376..9071
                     /gene="pgl"
                     /locus_tag="WIB30_000987"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ3797070.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            9052..10038
                     /locus_tag="WIB30_000988"
     CDS             9052..10038
                     /locus_tag="WIB30_000988"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ3797071.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFSPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGTSPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVCVVLSEFPGISGAAAALDNHLRNV"
     gene            10088..10936
                     /gene="hexR"
                     /locus_tag="WIB30_000989"
     CDS             10088..10936
                     /gene="hexR"
                     /locus_tag="WIB30_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ3797072.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            11057..12703
                     /gene="pgi"
                     /locus_tag="WIB30_000990"
     CDS             11057..12703
                     /gene="pgi"
                     /locus_tag="WIB30_000990"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ3797073.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEV
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            12717..13268
                     /locus_tag="WIB30_000991"
     CDS             12717..13268
                     /locus_tag="WIB30_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ3797074.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSELACKTVETEARLEELY
                     NRKKALIDEMVREADKKELPKRL"
     gene            13313..13453
                     /locus_tag="WIB30_000992"
     CDS             13313..13453
                     /locus_tag="WIB30_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797075.1"
                     /translation="MPEYRNWQNGLNAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
BASE COUNT         3337 a         3464 c         3728 g         3236 t
ORIGIN      
        1 ctaaatatct agcaaaaaag tgcggtcaaa attcaccgca cttttcgttt cgaacaatcg
       61 aattatttaa tcacgccaca tgccatacgt gggccgccac cgccaagtgg agctggatga
      121 tcggagtgat tatcaccacc cgtgtggatc ataatagagt gaccgcgaac atcatctaaa
      181 tgtttaagac gtggtgctaa aacaggattt gttgctgtgc catcatgcaa tacagttaat
      241 gcaggtaaat cacctaagtg tgcatcatct tgccatgggt aaccatgttg ttttgcacct
      301 ttaggatccc agtgaccgcc tgcgcctaaa ccagctgtca atttaccttc tttttctttt
      361 ggctcacagc ttgggttttc atggatgtgg aaaccatgta agccttcgct taatccttgt
      421 aaatcagggg taaacacaag accatagtta gattcagtaa tagtcactgt acccacatct
      481 ttgttaccgt ttactggatc aagttgttgc actttcactt caatagaagc acctttaggt
      541 atcgtattat gctcgtgtgc ttgcgcaaca ccaactgaac atactgcact aaccgctagt
      601 gctaataagg ttttcatatt cattgcaatc tcctttattg cagtaaaaac tcgcgactat
      661 tttagtcaat tttgttttaa aagccgataa atttttacga aaattttaca ttaattgtta
      721 tttgcagaaa attagaaccc gttatcacgt cttggataaa tgccaaaacc attgtgattc
      781 cgagaattaa aaagaaaaat ttcggaatcc tcatcctttt ctgaaacctg attgcggtat
      841 ccatggcttt gtctttgtca tttaagtttg tgttcatgtt tttgaccttc tttgcttttt
      901 aaatcctttt taaattgccg acttcttccg ttccaaatgt cccatttgaa cattatcccg
      961 ccgcctaaaa tacgacccaa attcatcggt tgttcttgct gcgatacctg caaacaacag
     1021 ttcaatgggt ttattttgtt tattctaacc tattaccgtt taaataaatt tccaaaggct
     1081 tgggcaaacc gtaatctttc aaatgcgccg gctttatcca aatgccgtct gaagggcttt
     1141 cagacggcat ttgtgcttca aagggcgtaa tcaatagcag ccggtgcgtc aggcggtggg
     1201 tcagggcggt ttgttcgtcc atatctgcca tggtcaggga gaatttggcg gcaaagtcgg
     1261 aaagcccgtt caaactttca aaacacggca cgcaatacag cccgccccaa atgcctttgg
     1321 cggggcgttt ttccagcaaa atcgcgccgt cccggttgcg gacaatcagc cagtaaagcg
     1381 gcagggtcgg tacttcggcg gcggtttttt tgcgcggcag ctcggcggtg cggttttgct
     1441 ttttcgcttc gcagatgtcc gccatcgggc attggtggca caagggtttc gtccgtttgc
     1501 acacggtcgc gcccaaatcc atcaaacctt gtgtataggc aggcatatcg gcgttttcag
     1561 acggcagcag gctttcggca agtgtccaga gcgagttttc aaattttttg tcctgcggat
     1621 tgccgtcgcg ggcgaacacg cggcagagta cgcgtttgac gttgccgtcc aaaatggttt
     1681 cgcggcggtt gaaggagaag gcgcaaatgg cggcggcggt gcttctgcct acgccgcaga
     1741 gggtttccaa gtctttgcgc tccgacggaa acgtgccgcc gaattgcctg acgacttgtt
     1801 gcgcggcttt gtgcaggttg cgcgcgcggc tgtaatagcc caagcccgcc cacaacgaca
     1861 acacttcgtc ttgcggcgcg gcggcaagcg tctgaacggt cgggaatttt tctaagaagc
     1921 gcggatagta gtccaacacg gtggcgactt gcgtttgctg gagcatgatt tcggaaagcc
     1981 agacgcaata agggtttttg acctgccaag ggaggtggtg gcgaccgtgt tgtttttgcc
     2041 agcggatgag ccgttcggag aaggggatgg gtgtgttcat taatatcaat cggtggtttt
     2101 atttatattt aaaacggtat gttattgtat aacattgtga aaataattct tattgactta
     2161 ttttttgtag gggcatataa ctcatataaa gaaactttat tgcggtattg aaattattta
     2221 tcaacaagca aggagtatca gcatgaaagc aatggtttat cacggcgcaa acgacatccg
     2281 ttttgaagaa aaaccccgtc cgcagattat cgatccgacc gatgcggtgg tgaaaatcgt
     2341 caaaaccacg atttgcggta ccgatttggg tatttggaaa ggcaaaaaaa acccgaagtc
     2401 gccgacggcc gtattctcgg tcatgaaggc atcggtattg tagaagaagt cggcgaggct
     2461 gtaaaaaaca tcaaagtcgg cgataaagtc attatttcat gcgtcagcaa atgctgtact
     2521 tgcgacaact gcaaaatcca actttattca cactgccgta acggcggctg gattttgggc
     2581 tatatgattg acggtacgca ggccgaatac gtccgtaaca gcctcgtgcc gctgcccgac
     2641 aacgtcaacg aagaagtcgc cctgttgttg agcgacgcct tgccgaccgc ccaactgtac
     2701 agccctgccg ccatcatcgt ttgcgatatg gacgaaaacc gtttgaaact ggcgaaagag
     2761 ttgggcgcga cccataccat cagccccgct tccggcgacg tctccaaaca agttttcgcc
     2821 atcgtcggcg aagacggcgt agattgtgcc atcgaagcgg tcggtatccc tgcaacttgg
     2881 aatatgtgcc aagacatcgt gaaacccgac ggccatatcg ccgtcgtcgg cgtacacgtt
     2941 caatccgttg atttcaaact ggaaaaactg tggattaaaa atcttgccat cactaccggc
     3001 ctggtaaacg ccaataccac cgaaatgctg atgaaggcaa tttccagcag ctccgtcgat
     3061 tacaccaaaa tgttgaccca ccgtttcaaa ttcagcgaat tggaaaaagc ctacgacgtg
     3121 ttcaaacacg ccgccgaaaa tcaagcgatg aaagtggttt tggaagcgga ttaatcggcg
     3181 ttgaatcagt tgttttttaa gcggatgccg tctgaatgcg tttcagacgg cattttttca
     3241 gctgcgtaaa acgggctgtc cggctgctgt cgaatgaact gaacgttatt tttattatgc
     3301 ggaaggcgga tgggcatttt aggggcggta tgtctgatca gtgctgccgt gtatgccgac
     3361 tgtgccctca atcggcaaaa tgccgtctga aacgggaaag ggttttagac ggcattttta
     3421 cgatgcggtt caggctttgg gttttaacgc cgccgcttct ttggcgaggc gggtgatggt
     3481 gtcccagtct ttgtttttca cagcttcttt cggggtcagc caagagccgc cgacgcacaa
     3541 gacgttgggc agtgccaggt aatcgggtgc ggtggcgagg ctgatgccgc cggtcgggca
     3601 gaagcgcacg tcggcataag gaccgtaaag ggctttgagc atggctttgc cgccgacgac
     3661 ttcggcgggg aaaagtttga gggtgtcgat gccgtgttcc aaagccagtt ggacttcgcc
     3721 cggagtggca acgccgggaa tcagggggat gccgctgttg tggctggctt tggcgaggga
     3781 ttcgtgcaaa cccgggctga tggcgaaaac cgcgcctgcg tcttcgacgg ctttgagctg
     3841 ttcggggttg gtaaccgtac ctgcgccgac gatggcgttg ggtacttctt tggcaatcag
     3901 gcggatggcg tcgaggccga cgggggtgcg cagggtaatt tcgagggtgg ggatgccgcc
     3961 ttcgacaagg gcgtgggaca aatcgacagc ggtgctcaag tcgtcaatcg ccatcacagg
     4021 cacaactgcg cctgcgctca ggatttcgcg gggggtcagt ttggacattt cggttctccg
     4081 ggtggaatgg ggtattttat taagatggga caggtcgtag gtcggattca agaaaccgac
     4141 atcttggacg gctgtgtcgg attcgagaat ccgacctacg tctatcttaa aacacttgaa
     4201 taatttttca gacggccttc ggtctgaaaa ggccgtctga aacgcgcatc aggcaaattc
     4261 gccgccgaaa ctcatggcac cggtttccgc gctgctggtc atgctgcgga agttggcgaa
     4321 gagttcgcgg ccgcagcctt gttggttcgc gcccaagtcg atgctttcga cttcgcgggc
     4381 attccattcg gtttcgttaa tcaggacgtt gagttcgccg ctaacggagt cgaagcggat
     4441 caggtcgccg gtacggattt tggcgatgtt gccgcccatc agggcttcgg gtgtcatgtg
     4501 gatggaggcc ggaactttgc cagatgcgcc ggacatacga ccgtcggtca gcagtgccac
     4561 tttgaagccg cggtcttgca ggatgcccaa aggcggggtc agtttgtgca attcgggcat
     4621 accgttggca cgcgggcctt ggtagcggac gacgcagacg aaatcgcgtt ccaactcgcc
     4681 gcgttcaaat gccgccaaca cttcgcgttg gtcgttgaac acgatggcag gcgcttcaat
     4741 aatgcggcag ccttcgcgca cggcggacac tttaatcacg ccgcgtccga tgttgccttt
     4801 catcaggcgc agaccgccgt cgggggagaa cgggttgccg gctttgcgca ggatgtcgtc
     4861 gttgccgctg gtttcggggg cttcgcgcca ttcgagtttg ccgtcgataa ggaaaggctc
     4921 tttggtgtag tggcgcatac cgtgtccgac aacggtatcg acatcgtcgt gcaacaggcc
     4981 tgcgtccagc aattcgcgga taacgaaagg tagaccgccg gcagcggtaa agtggtttac
     5041 gtcggctttg ccgttggggt acacacggat cagcaatggg atgatggaag aaatttcgtc
     5101 aaagtcgtcc cagttcaaaa tcacgccggc ggcgcgtgcc atggcaacga ggtgcatggt
     5161 gtggttggtt gagccgccgg ttgccatcag gccaatcaag gcgttgataa aggatttttc
     5221 ggtcaacatt tcgcccaaag gtttaatcgt gccgtttttg atgccgcgcg cgaggtgtcc
     5281 ggcggcgtag cgggtcagcg cttcacgcag gtcggtgtaa gggtggacga aagcggcggc
     5341 agggaggtgc acgcccatca tttccatcat catttggttg gagtttgccg tgccgtagaa
     5401 agtgcaggtg cccgggctgt ggtaagaacc catttcgctt ttcaaaagtt cgttgcgtcc
     5461 gaccttgcct tcggcgaaaa gctggcgggt gcgggctttt tctttgttgc cgataccgct
     5521 ggacatcggg cctgcgggga cgaagatacc cggaatatga ccgaacgaaa gcgcgcctat
     5581 catcaaacct ggaacgattt tgtcgcatac gcccataaac aggctgccgt caaacatttg
     5641 atgcgacagc ccgatggcgg tactcatggc aatcacgtcg cgggagaaca gcgacaattc
     5701 catgccggcg tagccttgcg tgatgccgtc gcacatggcg ggcgtgccgc cggcgacttg
     5761 ggcggtcgcg ccgtttttct gcgcttcgtc tttgatttgg tcagggaagt ctttaaacgg
     5821 ctggtgtgcg gaaaccatgt cgttgtaggc ggtgatgatg cctaagttgg ggacggtttc
     5881 ctgaagcatt tcgattttga tacttttagg catggcagca tagccgtgtg ccaagttgct
     5941 gcagccgagc tggttgcgct ctaagcgtcc catttgtttg gcactgcgga tcttcgccag
     6001 atatttttca cgcgtcggac ggctgcgttc aataatgcgc ccggtgattt cggcgagttt
     6061 ggagtggata ggagtagtgt tcacggtcgg tctcctgtcg gaacgggggg gtattgggga
     6121 tttgtttgtg cgtttctgat ttttgtatcg ggtattcaga cggtaagtat agtggattaa
     6181 caaaaaccag tacggcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg
     6241 aagcaccaag tgaatcggtt ccgtattatc tgtactgtct gcggcttcgt cgccttgtcc
     6301 tgatttttgt taatccacta tattttaaaa ggtatgccgt ctgaaagtgg gaagcgggtg
     6361 ttattatagt acaaaatttg taattttgtt acaaaaaaat caggcattga aaagaaattt
     6421 gatttaaatc gcagaatctt tcgattgttg taacggaata ggggaaacct gcccgatttt
     6481 tggactaggt caaataaatt gtaaaaatgg gaatagacaa aaagtgtagc ggtgtgtagt
     6541 attactactc attatgccgc ctgcctttgt ttccgatatt gcgatgcagc gtgatggggg
     6601 aaacaggttt cagacggcat cgaaaaattc tttcctgacg agataatacg atgagtacac
     6661 aaacaaattt tgatttggtg ttgttcggtg cgaccggcga tttggcgatg cgcaagctgt
     6721 tgccctgtct gtaccaggcg catgttgccg gtctgcttca tcccgaaggg cgtattttgg
     6781 gggtcagccg cagcgagttg gataccgaag gttttctggc gaaggtggaa accagttcca
     6841 aaatccatgt gaaagaaaat ttttccgatg aggcttgggc atcgtttgtc gaacgtttcg
     6901 catatctcaa agtcgatgtt acgcagccgg acgattttgc cgctttgggt gatttggtca
     6961 aagcgcgcaa agaaaccgac aatgtcgtca tctatctttc caccgcgccg aaattcttcg
     7021 cgcaagcctg tgaaaacctt gctgcgatcg gtttgaatgc cgacaatgtg cgcgtagttt
     7081 tggaaaaacc gttgggtacg gatttggcat cttcccaaca aatcaatacc gatgttgcgc
     7141 gttatttcaa agaagggcaa atctaccgta tcgaccatta tttaggtaaa gaaagcctgc
     7201 aaaacctgct ggccttgcgt tttgccaatg tgatgttcga gccgctttgg aacaataaat
     7261 acatcgaaag cgtgcagctg accatcgccg agcagttggg cgtggaagaa cgcggcgagt
     7321 tttacgacat taccggcgcg ttgcgcgata tggtgcaaaa ccacttgatg caaatgctgt
     7381 gcatgactgc gatggaagcc cccgccagct tggatgccga cgcggtgcgc gatgaaaaag
     7441 tcaaagtcat caagtcattg aagccgctga ccgtcgaatc tgtcaatgaa aatgtcgtgc
     7501 gcggacaata taccgccgcc aaaggcatga acggctatct tgaagaaatc aacgttccgc
     7561 aagacagctt taccgaaacc tacgtcgcca ttaaagccga aatcgaaaac gaacgctgga
     7621 agggcgttcc cttctacctg cgtaccggca agcgcatggc gggcaaagtg gcggaaatcg
     7681 ttttgaactt caaagatttg aacagccata tttttgaagg cagtcgcacc gcgcccaacc
     7741 ggctcgttat cgagttgcaa ccatatgaat ccgtgcgcct ctatacgcag atgaaaaccc
     7801 cgggggcagg aaataaggtc gaaaccgtgc cgctggcaac cgatttgggc aaagcattgg
     7861 aaagccgccg cgcggaagct tacgagcgcc tgctgctgga tgtgattaac ggcaaactcg
     7921 ctttgtttaa ccgccgcgac gaacttgaag ccgcgtggga atatgtgatg ccgattttgg
     7981 aaaactggac aaataacacc acgccgccgc acggctatgg cgcacactcg tgggggcctg
     8041 aagccgcgcg cgaactattg gcgcgcgacg gacacaagtg gcacgaagag cagtaataca
     8101 ataatgcgtt cagacggcat ggggtttgaa atgccgtctg aacgtaagta aagcagtata
     8161 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggca
     8221 aggcgaggca acgctgtact ggtttaaatt taatccacta taaaaagcag tcccgatatt
     8281 tggtatcaaa agcataaaca actgttcgac cggcatattg ctcaacccgt ttccataacg
     8341 gaatatgccg tctgaaataa aaaaaggaca caaatatgtt tgtttggcac gaatacgaaa
     8401 atgcggcaga agcggcgcag tctttggctg acgcagtggc ggatgctttg cagggcgcac
     8461 tggacgagaa gggcggtgcg gtgttggcag tttccggcgg acgttcgccg attgcatttt
     8521 tcaacgccct gtcgcaaaaa gatttggatt ggaaaaacgt cggcatcacc ttggcagatg
     8581 aacgcatcgt gccgaccgtc cacgccgaca gcaataccgg tttggtgcgc gaatacctgt
     8641 tgaagaacaa agcggaagcg gcaatgtgga ttcctatggt ggaagacgga aaaactgaaa
     8701 ccgaattaca tcccgatgct gttgtcgatt atgcactgaa acattacaaa cagcccgatg
     8761 ttttggtttt gggtatggga aacgacgggc atacggcttc gattttcccg aaagctccac
     8821 agtttcagac ggcaatcgac ggttcggcag gtgtcgcgct ggtgcatacc acgcccgtta
     8881 ccgcgccgca cgagcgcgtc agtatgacct tggatgcgat tgcccatacg gggcatgtgt
     8941 ttttggcgat acggggcgaa gagaaaaaag ccgtgttcga ccaagccgca caaggcgaaa
     9001 accgcgaata tccgatcaac ctcgttttga accatcaagg agtgaactgc catgtcttct
     9061 acgccgaata aacaagccgg atatccccga ctggtcgccg atatcggcgg gacgaatgca
     9121 cgctttgcgc tggaaaccgc gccgcgcgtc attgaaaaag ccgccgtgct tccgtgtaaa
     9181 gactacgata cggttaccga tgcggtgcgt gcctatctga atcaaagcgg tgcaacagcc
     9241 gtacggcacg cggcatttgc catcgccaac ccgattttgg gcgactgggt gcagatgacc
     9301 aaccaccatt gggcgttttc catcgaaacc acccgtcaga ctttggggct ggacaccctc
     9361 atccttttga acgactttac cgcgcaggca ttggcggtaa cgcagacttc aagcaaagac
     9421 ctgatgcagg taggcgggca aaagcctgtc gaatttgccc ccaaagccgt tatcggcccc
     9481 ggtaccggcc tgggcgtgag cggattggtg cacagccacg caggctgggt ggctttggcg
     9541 ggcgagggcg ggcataccag tttctcgccg tttgacgata tggaagtgct gatttggcag
     9601 tacgccaaaa acaaatacgg ccatgtttcc gccgaacgct ttttgagcgg cgcgggcttg
     9661 agcttggttt acgaggcttt ggctgcaaaa cagaaagcca aacccgccaa actgatgccg
     9721 tctgaaatca cggaaaaggc tttgagcggc acgtcgcctt tgtgccgtca gactttggac
     9781 atcttctgcg ccatgctcgg cacggttgct tccaacctcg ccctgacgct gggcgcgcgc
     9841 ggcggcgtgt acctgtgcgg cggcattatt ccccgcgtgt tggaatactt caaaacttcc
     9901 ccgttccgca gccgtttcga gaacaagggc aggtttgaag catatcttgc cgcgattccc
     9961 gtgtgtgtcg tcttgagcga gtttcccgga atttccggtg cggctgcggc tcttgacaac
    10021 catttgagaa acgtttaacc acagcggctc cttgcagcgg ggctgcatta tcgaagggca
    10081 tatcattatg ttaagcaaaa tcagcgaatc actggcaaac ctttccggtg cggaacgcaa
    10141 agtcgccgaa tgtgcattgg cggaacccaa atggttcgtc catgccgccg ttgccgaaat
    10201 tgccgaacgt gcgtccgtca gccagccgac cgtcatccga ttctgccgca gcttgggtta
    10261 taaagggctg cccgagttca agctcgcctt gtccgccagc atcggtcatg agggtatgcc
    10321 ctatgtccac gaagaactca acgccgacga cgatatggca agcgtggtcg agaaagtgtt
    10381 gggcaatgcc gccgcctcgc tgttgggcga acgccgcttc ctgaaagagt cggagctgga
    10441 aaacgccatt gccacgctga tgcacgcccg tcgcgtcgag ttttacggtg tcggcaattc
    10501 cggcattgtg gcacaggacg cgcagcataa atttttccgt ttcggcatgt ccaccgtcgc
    10561 ctatgtcgat acgcacacgc agctgatggc ggcatctgtt ttgagcgatc aggatgtttt
    10621 ggttgccatt tccaacacgg gttcgtctat cgaacttttg gatgcggtca gcatcgccaa
    10681 agaaaacggc gcgtctgtca tcgcactgac ccgcaacgat tcgcctctgg cgcaacttgc
    10741 cgactgcgtg ttgagcgttg ccacacagga aaatgccgaa ctctacacgc ccatggtttc
    10801 ccgcctcttg cagcttgccg tcatcgacat tctcgccatc ggacttgccc tgcgcttggg
    10861 cgatgctgcc agcctgcaat tgcagaaaag caaaaaaagc atacacaaca agcacatcga
    10921 ttacgacaaa gattgacctt cagacggcat cccacaaatg ccgtctgaaa tgccgaacaa
    10981 cggtcgtcgg cggcttgcgg cagtttccgg cagccttttc atcccacaac aaaaaacctc
    11041 attcaggagc atatagatga aacaccttca cgacttaccc gcatggtcga aattgtggaa
    11101 tcactttgac gacagcaaaa cattgcatat gcgcgaaatg ttcgagcaag acccgcagcg
    11161 tgcggaacgc tactggctgc aagtcggcgg actgacgctg gactactcca aaaaccgcat
    11221 caacgacgaa accatgtcgc ttttgttcga gcttgcccga gaagcaggcg tgccggagcg
    11281 gatgcggcag atgttccacg gcgaaaaaat caataccacc gaaaaccgcg ccgtcctgca
    11341 tgtcgccctt cgcaaccgca ccaattcgcc gattgtggtt gacggtgaag atgtgatgcc
    11401 caaagtcaac cgcgttttgc aacgtatggg cgaatttgca cacgaagtcc gcagcggaag
    11461 ctggctgggc tataccaacc aagtcattac cgacgttgtc aacatcggca tcggcggatc
    11521 ggatttgggt ccgctgatga tgtgtaccgc gctcaaacct ttcggtcatc cgcgcctcaa
    11581 tatgcacttc gtctccaacg tggacggctc gcaactgcgc gacgtattgt ccaaagtcca
    11641 ccccgaaacc acgttgttca tcatcgcctc caaaacattt accacgcagg aaacgctgac
    11701 caacgcgctg accgcgcgcg aatggttttt gaatcatgcg ggcgacgaag aagccgttgc
    11761 caaacacttc gccgccgttt ccaccaatca aaaagccgtc gccgaattcg gcatcgacac
    11821 cgccaatatg tttgaatttt gggattgggt cggcggtcgg tacagcctgt ggtccgccat
    11881 cggattgccg attatgctgt atctcggcga agaaaacttc attgaaatgc tcaacggcgc
    11941 gcacctgatg gaccaacact tcatcaacac accgctcgag cgcaacctgc ccgtcattct
    12001 cgccctcatc ggcatctggt atatcaacta ctacggcggc ggcagccacg tcatcgcgcc
    12061 ttacgaccaa catttgcacc gcctgcccaa attcatccag cagctcgata tggaaagtaa
    12121 cggcaaacag gttacgttgg acggcaaagc agtcggacac gaaacctcgc cgattatctg
    12181 gggcgaaacg ggcattaacg gccagcacgc ctttttccaa ctgctgcacc aaggcacgca
    12241 cattaccccc atcgacctga ttgcctcgct tgaaaaacgc agcaacctgc ccggacacca
    12301 cgaaatcctg cttgccaacg tcttcgccca agcagaagcc tttatgcgcg gcaaaacccc
    12361 cgacgaagtc cgcgccgaac tcaaagcgca gggtatggat gaggtgcgca tcgaagagct
    12421 ggtcccgcac aaaaccttct ccggcaaccg cccgaccaac ctcattctca tggacaaggt
    12481 caacccgcgc aatatgggca gcctgattgc catgtacgaa cacaaaacct tcgtacaagg
    12541 catcatttgg ggcatcaaca gcttcgacca gtggggcgtg gaactcggca aacaactggc
    12601 taaaaccatt ttgggcgaac tgaccggcga aaccgggccg caaaagcacg acagttcgac
    12661 cgaacgcctg atcaacctct acctgcagac caaccgcaaa taaaacctgc ggaaaaatgc
    12721 cgtctgaacg ccgaccgttt caaacggcat ttttatcgaa caggaaaacc gtcggtaaac
    12781 ttgcagagcg tgtgcaatcc cgatatgatg gtttgcataa atttaaacat atatgttccg
    12841 cagctatggc actgattaaa gagccgttgg acaaagcgaa acaaaggaac gaagaacttg
    12901 aagcggcaga agaagcggcg gcgcaggagg cattgggtcg ggagcaggaa gccgcccgcg
    12961 tatccgaatg ggaagaacgc tacaagttgt cgcgcagcga gttcgagcag ttctggaaag
    13021 gattgccgca aactgtacag aataaactgc aagcttcgca gaaaacatgg aaaagcggta
    13081 tggacaaaat ctgtgccaac aatgcgaaag ccgaaggtga aacgccaaac ggcataaaag
    13141 tcagcgaact ggcatgcaaa acggtggaaa ccgaagcacg cttggaagag ctgtacaacc
    13201 gtaaaaaagc ccttatcgac gaaatggtca gggaagcgga caagaaagaa ctgccaaagc
    13261 ggctctgaac agcgcggttc aggcactgcc cgccgatatt gccgaaaccg tcatgcccga
    13321 gtaccgcaac tggcaaaacg gtctgaatgc caaatgcgct gacggcgacg aatacggcgt
    13381 ggcacaatcc gactgccgta ccagagaaat caatgcgaaa accaaagaaa tccaaggtta
    13441 tctgattgac tgaaacttgg atgcgggaat gtcggcggct tttgcgtttt tgtcgttttt
    13501 atagtgaatt aaatttaaac cgatacatcg ttggctcgcc ttagctcaaa gagaacgatt
    13561 ctctaaggtg ctgaagcacc aagtgaatcg gtccgtacta tttgtactgt ctgcggcttc
    13621 gccgccttgt cctgattttt gttaatccac tatatctttt tccacttatc tgcgctttca
    13681 ctatttgccc tttcaggctg cgggcatagg gacggaacag gtagcggtca aatcctgttt
    13741 catccaaata aacacgttgg tagtc
//
LOCUS       DAWHRA010000046        13541 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWHRA010000046 DAWHRA010000000
VERSION     DAWHRA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.046"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(24..715)
                     /locus_tag="WIB30_000993"
                     /pseudo
     CDS             complement(24..715)
                     /locus_tag="WIB30_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1021..1578)
                     /locus_tag="WIB30_000994"
     CDS             complement(1021..1578)
                     /locus_tag="WIB30_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797076.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWGYHGANLYNHGKLGTAQGAATATGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1822..1980)
                     /locus_tag="WIB30_000995"
     CDS             complement(1822..1980)
                     /locus_tag="WIB30_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797077.1"
                     /translation="MKIPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(1977..2819)
                     /locus_tag="WIB30_000996"
     CDS             complement(1977..2819)
                     /locus_tag="WIB30_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3797078.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3058..3978
                     /gene="pyrB"
                     /locus_tag="WIB30_000997"
     CDS             3058..3978
                     /gene="pyrB"
                     /locus_tag="WIB30_000997"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ3797079.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWNCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3988..4446
                     /locus_tag="WIB30_000998"
     CDS             3988..4446
                     /locus_tag="WIB30_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ3797080.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4511..5176
                     /locus_tag="WIB30_000999"
     CDS             4511..5176
                     /locus_tag="WIB30_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797081.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQILAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5239..6456)
                     /locus_tag="WIB30_001000"
     CDS             complement(5239..6456)
                     /locus_tag="WIB30_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ3797082.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6598..7143
                     /gene="def"
                     /locus_tag="WIB30_001001"
     CDS             6598..7143
                     /gene="def"
                     /locus_tag="WIB30_001001"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ3797083.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7230..8156
                     /locus_tag="WIB30_001002"
     CDS             7230..8156
                     /locus_tag="WIB30_001002"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ3797084.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSKS
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGAKL"
     gene            8235..9494
                     /gene="rsmB"
                     /locus_tag="WIB30_001003"
     CDS             8235..9494
                     /gene="rsmB"
                     /locus_tag="WIB30_001003"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ3797085.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLMAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLVASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASTACADAQDLSAWYDGKPFDAVLADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDEQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9460..10059
                     /locus_tag="WIB30_001004"
     CDS             9460..10059
                     /locus_tag="WIB30_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ3797086.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10059..12179
                     /locus_tag="WIB30_001005"
     CDS             10059..12179
                     /locus_tag="WIB30_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ3797087.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGRLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSSSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEAGRDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            12172..13449
                     /locus_tag="WIB30_001006"
     CDS             12172..13449
                     /locus_tag="WIB30_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ3797088.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSTVENALKYGAAQTETGPVFDKLGNSAAIQEMNCEVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
BASE COUNT         3201 a         3441 c         3869 g         3030 t
ORIGIN      
        1 ataaatgggc aaaaatcggt ttattatcgt ttttgccgca tttggatttg ttctaccgta
       61 aaacgtgttt gacgaacggg attcttatta aaaaacatct gatttctaac aaaatcagta
      121 ttttttggca cgatggctaa aatttttcct tccatttcgc catcacgtgt tttccatgcg
      181 ctcaagaatt gtgatttgct cattgagacg tgccccagcg atggatcagc cagcaaaaca
      241 gtttctccgt taataccgtt caataccgaa aaatggttgt ttttacggta ttttaaatac
      301 acaattacag gaatttttag ttgtaccaac tgttcaaatg gcaaagcata accttgtgct
      361 tcaaaaccca gttcgggcat tatgcgttgc atatcgtcaa aagaagcacg catttgggtt
      421 ttatccattt tgtctaagat ttccgcttca gaataatgtc tgccataaaa attattcagt
      481 aacgtggcaa tcgaagccgc gccgcaagaa aaatccaaat cttgttttac tatgccggaa
      541 tctcgccgtg ctttccaact ccgtacatgg atgttttggt aagaagcggg gggtcaacaa
      601 acataggcca agcaaaaact atatttgggg cgaaaccaat caaagccgca taatttatca
      661 atttataaag attttttatc ataatatgta tacgcggaat aaacgcatga aacataaaaa
      721 aataaaatca tatgcaattt tattgcatga ataaatatga ataaaataga taatgatggg
      781 agtaaatacg ccatgtattt tggaagttta aatttattaa taataaaata atttatcgta
      841 gcgcaaataa atcccaaaat tggaacgatt agaaaaaaaa ttaatacacc catcatcatg
      901 caaactctat attaataaat agctaactaa ttctattgtg gaaattagtt agctaactaa
      961 aagttattaa tgattatttt cgagaattga ctgcattgtt ggcagcattg gcaccaaaac
     1021 ctagtgcatg aatacccggt ctccatgcca aattcccagc caatccgcct ccggcagcag
     1081 cagccagccc tgttttgccg ctactcctgt tgccgcaccg attcctgtcg cagtagccgc
     1141 gccttgcgca gttcctaatt taccatgatt atacaaatta gcaccatgat acccccatgc
     1201 acctaatgca ccgccaaaag cagcggctgc aataatggga acaaattcac cttgtgtttc
     1261 tttcatttca gcctgtgata attgaattgc tttcacattt tggctgtcaa aaacttggct
     1321 gtctaaattt tgcgccatta caggtgtaat catcatagcc attacagttg caattttcgt
     1381 tgcgctggtt tgcacataaa taggattagc aaattcgctt tgattgcgtt cagtgttgat
     1441 gtagctaata ctgctttcta gtttgaattt acccttgtca gtcaataatt cttccaaact
     1501 taaaggtaag tcagcataag caatttggct ggcaacagtc aaaataaaat ctattagaca
     1561 tttgtgtttt tgcatcattt cgtttgattt tctaggtttt gagaatgata caaagttttt
     1621 tacaaagtaa agagtcactc tgaaaaaact tttttcatta taaatcaaaa tattgataga
     1681 ataaatagcg agcatcgatt cacggtgcgc tttagtgcaa agcgtacaac gcgagcctga
     1741 accaccagcc gcaacaggaa aagaaagccg atagagtgca atgcttgcca acgtgcaagc
     1801 gagcttgcaa gaacgcttgg ctcaacgaga gcaggcaaga cagaaagcag aaagcaggat
     1861 aggagcggta acgcaaaggt ctcgggcttt gatttcgccg taaaccctgc tgccgccttg
     1921 tccggaaagg gtgcaggcgg cgagtgccga cagggtgcag atggggaggg ggattttcat
     1981 ttggggtcgc aacggaagtg gtatgcgcag atttcaaaac cgtttttgaa atacaggcgg
     2041 tgcgcgtcgg cacggtcgtg gttgacgtgg acgttgaggt ggattttggt tacccctgtt
     2101 tccgcgccga ttttgcggac ttcttccaaa aggcgcgagg cgtagccttt gcggcggctt
     2161 tgcggcaggg taacgatgtc atcgatgtgg atgtggcggc cgctggcgag ggtgcaggct
     2221 tcgcggaagc cgcagacggc gacggcattg tgtttgcctt cttcaaaaat acccagcagg
     2281 cggtagcctt gggggcgttg gactttgttg atctgttcgg taaagcggtt gatgtcggtc
     2341 agggcggaac gcaaaacgct caaggctgca aaggcggtgg cggtgtcgtc cgcgccgatt
     2401 tcgcgcaaaa cgtaggatgc gcccgaggcg gtctgttcct gtgctttctc ggcggcgtgt
     2461 ttttcttcga ttgcctgtgc cagcatgacg tgttcgtcgg cagggttgtt ttgtccgccc
     2521 tgttcgcgtt cttcgagcag ggctttgcag tcgatgacgc gcaggtcgtt gtcggcggca
     2581 aagtccatca ggaagcggaa catttgggga ttgtcggttt ccagtttttt atcgacggcg
     2641 acgcagcgga tgttgtccac cagtatgggg cgcacccatt tcgaatagga aagcctgtgg
     2701 tctttggtga acgaggacag aatgccgcac aggcgttccg cccagtcgct gggacggaaa
     2761 atcttgccgg aactcgttgt gccgtggatg acgacttcgt aggggttgca gactaacatg
     2821 gcggcttcct gaaaagaaat gtctagcgcg attatacctt atgcttatgc gggcgtgttt
     2881 ggatatgccg tctgaaaagt acgggattcg tgcggtaaaa ctttgcggcg gcaaatgtgc
     2941 gataatacgc gccgtattgc cgcttttgcg aagctgttcc gcaaacatac gggcggcgtg
     3001 gacgacgtat aaccggatac ccgcctgacg cgggtttttt acggaagggg ggcaaaaatg
     3061 cctaatccgc tttacagaca gcatatcatc tccatttcgg atttgtcgcg cgaacagttg
     3121 gaatgcctgc ttcagacggc attgaagctg aaggcgcatc cgcgcggcga cctgttggaa
     3181 ggcaaactta tcggttcgtg ctttttcgag ccgtccacgc gcacgaggct gtcgtttgaa
     3241 acggcggtgc agcgtttggg cggcaaggtc atcggtttct cggacggcgc gaataccagt
     3301 gccaaaaaag gcgagacgct tgccgatacc gcccgcatca tttccggata tactgatgct
     3361 atcatccaac gccaccccaa agacggcgcg gcgcgcgtgg cagcggagtt ttcgcgcgtc
     3421 cccgttatca acgccggcga cggcacgaac cagcacccca gtcagacgct gctcgacctg
     3481 gttaccattt atgaaacaca gggacgtttg gacaagctca aaatcgccat ggcgggcgac
     3541 ttgaaatacg gacgtaccgt gcattcgctt tgtcaggcgt tgaaacgctg gaattgtgaa
     3601 tttgcctttg tttcgccgcc cagcctagcc atgcccgact atattaccga agagttggac
     3661 gaagccggct gccgataccg tatcctcggt agtttggaag aagcggcgga atgggcggat
     3721 atcctgtata tgacccgcgt ccagcgcgaa cgtttcgacg aacaggaatt tgccaaaatc
     3781 caaggcaaat tcaacctcga agcgtctatg ctcgcccgcg ccaaaccgaa cctgcgcgtg
     3841 ctgcaccccc tgccgcgcgt ggacgaaatc catcccgatg tcgatgccac gccgcacgcc
     3901 tattatttcg agcaggcgac caacggcgtt tatgcgcgta tggcgatatt gtcgctggtg
     3961 ttgaacgaag aagtgtgagg aaccgatatg gaaaccccga aactcagtgt cgaagccatt
     4021 gaaaaaggta cggttatcga ccatattccc gccggcaggg ggctgaccat cctgcgccag
     4081 ttcaaacttt tgcactacgg caacgcggta accgtgggct tcaacctgcc cagcaaaacc
     4141 caaggcagca aagacatcat caaaatcaaa ggcgtgtgct tggacgacaa agccgccgac
     4201 cgcctcgccc tgttcgcccc cgaagcggtg gtcaacacca tcgacaattt caaggtcgtg
     4261 cagaagcggc atttgaacct gcccgacgaa atcgccgaag tgttccgctg tccgaacacg
     4321 aattgcgccg gccacggcga gccggtcaaa agccggtttt atgttaaaaa gcacaacggg
     4381 cagacgcggc tgaaatgcca ctactgcgaa aaaacctaca gccgggattc ggtggcggaa
     4441 gcctgacgga ttcccttaaa ccgagtgggc ggcatttcgt ctgccgcctg ttttgccaat
     4501 ctgaaatgga atgatgatgc acgcttctgt ccaaagccgt ttcgcaccga tactttatgt
     4561 tttgattttc tttgccggtt ttttgaccgc gcaaatctgg ttcaatcaga aagcctatac
     4621 tgaagagctg cctccgcttc tgtccgcatt gtccgccgtc gcgctggtgt ggctggcgtg
     4681 ggcgttcgtg tcggcgcgtt caaaggccaa ggcggaaaag ttctaccgcg aaaaaatgat
     4741 acagaacgaa agcatacacc ccgtcctgca cgcctctttg caacacttgg aacacaagcc
     4801 gcaaatactc gccctgctgg tcaaaaacca cggcaaaggg atggcggaac aggtcaggtt
     4861 caaggcggaa gtgctgcccg acgacgaaga cgcgcgcacg attgccgccg agttggcaaa
     4921 aatggatatg ttcgcattgg ggacggacgc ggtcgcctcg ggcgaaacct atggacgcgt
     4981 gttcgccgat attttcgagt tgtcggcggc tttggaaggg cgcgcgttca aaggaatgtt
     5041 gaaactgacg gcggaatata aaaacatctt cggcgatgcc tgccgttcgg aaacggcgtt
     5101 ggagttgggc gcactcaatc aggcgttgca ggagatttca aaaacatcgg aaaagtccaa
     5161 acggatattt tattgaagat ggaaaaatgc cgtctgaaac ggaaggtgtt tcagacggca
     5221 tttttgtcgg atgattaatt attcggagcg gttgaagcca aacttcacgc ggctgcggcc
     5281 ctgatccggt atattgtcca aatcgcgtcc cggattggcg gcggtgtcgc ctacggaaat
     5341 atcggagatg ttttccaaaa tgatggcgga cgacaggtgt tcggaggtgc ggtaaaccat
     5401 tgccaagccc acttcttcgg caggagtgga aatcagctcg acggtatccc tgcttttgaa
     5461 attgttggag aggtcgacct gcatcgtttt cttgcgtttg tagaggctca aaaccgtgcc
     5521 tttgtccaaa ccgtccgcct cgcctttgtc gatggtgatg gtttgaaact ggccggcaat
     5581 ccttgtgcct tcaaacacgg aaacgatttt agcctgaacc gggcgggacg gttcgtgcgg
     5641 catcatgttg aagcggtcgg tgtcttccgg cattttcatc aggtagtcgc cctgctgtat
     5701 ttcggaaatg gcggtttcga ccaccagcgg ctgtatcgaa ggggtgcgca gcggggtaat
     5761 caaaggatgg gtgcgggtat ggtattcgtt gtctttcggc cgttctccag cctgtttcga
     5821 gcgttgttcg aggacagagt cggtatagtc gagggagcgc acgatgccgc tgaatgcgac
     5881 ttcctgcccg aggaatttac ccgtatccgg atcggtgatg tttttattga ttcggtaggt
     5941 caggtagcgg cccggctctt tcaggccttt ggtgtaaacc ctggtgcctt tggtgtacag
     6001 cagcctgcct tccgggcccg agagcaggcg cggcgcggca gcggtttctt tgcgggaaac
     6061 gatttgcgga tgccgcataa agatgcggta gaagttgaca tcgatggcgg gaataccgta
     6121 tccggacact tccttatccg gactcatttt gacgacgggg atgccgtctg tctgttccaa
     6181 gccgaggcgc ggttcgccgt caacgtggcg caacaccaat acctggtccg gataaatcag
     6241 gtcgggattg tggatttgat cccggttcgc gtcccacagg cggccccatt gccacgggct
     6301 gtacaggtat ttgcccgaaa tgccccacag ggtgtcgccc tgtttgaccg tgtagcgttc
     6361 cggcgcgttc gggcgcacct ccaaatttgc cgccaaagtt tgtgttgaga atgccatacc
     6421 tgccgcgcag agcagggtta taatacgacg ttgcataacc gttcccctta tctgataaat
     6481 ttcggtttgt cttgcttgat tgggttggaa aaagcggcgg cagcccctcg ggatgtgccg
     6541 cgtgataaaa aatgttccgc attttaacat cgaattatcc gcaccatcac ggtaattatg
     6601 aaaaacaggc ggcgtatccg ccgaaggaaa gagaaaatta tggctttatt gaatatcttg
     6661 caatatcccg acgagcgtct gcacacggtg gcaaagcctg tcgaacaagt cgacgagcgc
     6721 atccggaagc tgattgccga tatgtttgaa acgatgtacg aatcgcgcgg catcgggctg
     6781 gcggcgacgc aggtcgatgt gcacgagcgc gtggtcgtga tggatttgac cgaagaccgc
     6841 agcgaaccgc gcgtgttcat caaccccgtc atcgttgaaa aagacggcga aaccacttac
     6901 gaagagggct gcctgtccgt gccgggcatt tacgacaccg taacccgcgc cgaacgcgtc
     6961 aaggtcgagg ctttgaacga aaaaggcgaa aagttcacgc tggaggcgga cggcttgttg
     7021 gcgatttgcg tgcagcacga gttggaccac ctgatgggca tcgtgtttgt cgaacgcctt
     7081 tcccaactca agcaggggcg gattaagacc aagctgaaaa aacgtcagaa acatacgatt
     7141 tgaccctttt gccgtgccgt ctgaacgctg caaagttttc agacggcacg gtcttgtccg
     7201 acaattttac gcacgcgcag gaacacgcta tgaaagtcat cttcgccggc acgcccgatt
     7261 ttgccgccgc cgccttaaga gccgttgccg ccgccggttt tgaaattccg ctggtgctga
     7321 cccagcccga ccgtccgaaa gggcgcggta tgcaactgac tgccccgccc gtcaaacaag
     7381 ccgcgctgga actcggtttg cgcgtcgaac agcccgaaaa gctgcgcaac aacgccgaag
     7441 ccctgcaaat gctcaaagag gtcgaggcag acgtaatggt ggttgccgcc tacggtttga
     7501 ttctgccgca ggaagtgttg gatacgccga aacacggctg cctcaacatc cacgcttcgc
     7561 tgttaccccg ttggcgtggc gcggcgccga ttcaacgcgc gattgaagcc ggcgatgccg
     7621 agacaggcgt gtgtattatg cagatggaca tcggtttgga caccggcgat gtggtcagcg
     7681 aacaccgcta cgccatccaa ccgaccgata ccgccaacga agtccacgac gcgctgatgg
     7741 aaatcggtgc ggcggcggtt gttgccgatt tgcaacagct tcaaagcaaa ggccgtctga
     7801 acgcggtcaa acagcccgaa gaaggtgtta cttacgcgca aaaattgagc aaagaagagg
     7861 cgcgtatcga ttggagcaaa agcgcggcgg ttatcgaacg caaaatccgc gccttcaacc
     7921 ccgtgcctgc cgcgtgggtt gagtatcagg gcaagccgat gaaaatccgg cgggcggaag
     7981 tggtggcgca acaaggcgcg gcaggcgaag tgttgtcctg ttcggcggac ggtttggtcg
     8041 ttgcctgcgg cgaaaacgcg ctgaagatta ccgaattgca gcctgccggc ggcaggcgga
     8101 tgaatatcgc ggcgtttgca gcaggacggc atatcgaagc aggggcgaag ctgtaaatcc
     8161 cttcagacgg cattccgatc cgcaaacggg aatgccgtct gaaaccatca gtcgaagaaa
     8221 gcgaatcaca taatatgagt atggcacttg cccaaaaact tgccgccgac agcattgcgg
     8281 cggttgccga aggacgtaac cttcaggacg tgttggcgca aatccgcacc gcgcatcccg
     8341 accttatggc gcaggaaaac ggcgcgttgc aggacatcgc ctacggctgc cagcgttatt
     8401 tgggcagttt gaaacatatg ctcgcgcaga tgctgaaaaa gccgattggc aatccgcagc
     8461 tcgaaagcct gcttttggcg gcgttgtacc agctgcatta cacgcgcaac gcgccccacg
     8521 ccgtggtcaa tgaggcggtg gaaagcatcg cgaaaatcgg acgcgggcag taccgttcgt
     8581 ttgccaacgc ggttttgcgc cgctttttgc gcgaacgcga caagcttgtg gcttcctgta
     8641 aaaaagacga tgtagcgaaa cacaacctgc cgctgtggtg ggtggcttac ttgaaaaacc
     8701 attatccgaa acactggcac aacatcgccg ccgcgctgca atcccatccg ccgatgactt
     8761 tgcgcgtcaa ccgccgacac ggcaatgccg aaagctattt ggaaaaactg gtggcggaag
     8821 gtatcgcggc taaggcgttg gacgaatatg cggttacgtt ggaagaagcc gtgccggtga
     8881 accgcctgcc tggtttttca gacggcattg tttcggtaca ggacttcggc gcgcagcagg
     8941 cggcgtattt gttaaacccg aaagacggcg aacggatttt ggacgcgtgc gccgcgccgg
     9001 gcggcaagac ggggcatatc ttggaactgg cggattgccg tgttaccgcc ttggacattg
     9061 atgcaggccg tctgaaacgg gtggaagaca atatcgcgcg tctgggcttt cagacggcat
     9121 cgacggcgtg tgccgatgca caggacctgt cggcatggta tgatgggaaa ccgtttgatg
     9181 ccgtccttgc cgacgtgccg tgtaccgctt ccggcgtggc gcggcgcaat cccgacgtga
     9241 aatggctgcg ccgtccgacc gacgcgctca aaaccgcccg ccagcaggaa gccctgctgg
     9301 acgcactttg gcagacgctg aaacagggcg gcagaatgct gcttgccacc tgttccgtgt
     9361 tcgtcgaaga aaacgacgag caattgcaaa aattcctcaa ccgccatgcc gatgccgaac
     9421 cgatcgaatc gcgggtgctt ttaccgaaca aacaccaaga tggcttttat tacgcgctta
     9481 ttcaaaagca ttaaacaatg gcttgtgctg ctgccgatgc tttccgtttt gccggacgcg
     9541 gcggcggagg ggatagatgt gagccgcgcc gaagcgagga taaccgacgg cgggcagctt
     9601 tccatcagca gccgcttcca aaccgagctg cccgaccagc tccaacaggc gttgcgccgg
     9661 ggcgtgccgc tcaactttac cttaagctgg cagctttccg ccccgataat cgcttcttat
     9721 cggtttaaat tggggcaact gattggcgat gacgacaata ttgactacaa actgagtttc
     9781 catccgctga ccaaccgcta ccgcgttacc gtcggcgcgt tttcgacaga ctacgacacc
     9841 ttggatgcgg cattgcgcgc gaccggcgcg gttgccaact ggaaagtcct gaacaaaggc
     9901 gcgctgtccg gtgcggaagc aggggaaacc aaggcggaaa tccgcctgac gctgtccact
     9961 tcaaaactgc ccaagccttt tcaaatcaat gcattgactt ctcaaaactg gcatttggat
    10021 tcgggttgga aacctctaaa catcatcggg aacaaataat gcgccgtttt ctaccgatcg
    10081 cagccatatg cgccgtcgtc ctgttgtacg gactgacggc ggcaaccggc agcaccagtt
    10141 cgctggcgga ttatttctgg tggatagttg cgttcagcgc aatgctgctg ctggtgttgt
    10201 ccgccgtttt ggcacgttat gtcatattgc tgttgaaaga caggcgcgac ggcgtattcg
    10261 gttcgcagat tgccaaacgc ctttctggga tgtttacgct ggttgccgta ctgcccggcg
    10321 tgtttctgtt cggcgtttcc gcacagttca tcaacggtac gattaattcg tggttcggca
    10381 acgataccca cgaggcgctt gaacgcagcc tcaatttgag caagtccgca ttgaatctgg
    10441 cggcagacaa cgcccttggc aacgccatcc ccgtgcagat agacctcatc ggcgcggctt
    10501 ccctgcccgg ggatatgggc agggtgctgg aacattacgc cggcagcggt tttgcccagc
    10561 ttgccctgta caatgccgca agcggcaaaa tcgaaaaaag catcaacccg cacaagctcg
    10621 atcagccgtt tccaggtaag gcgcgttggg aaaaaatcca acgggcgggt tcggtcaggg
    10681 atttggaaag cataggcggc gtattgtacg cgcagggctg gctgtcggcg ggtacgcaca
    10741 acgggcgcga ttacgccttg tttttccgcc agccgattcc caaaggcgtg gcagaggatg
    10801 ccgtcttaat cgaaaaggca agggcgaaat atgctgagtt gagttacagc aaaaaaggtt
    10861 tgcagacctt tttcctggca accctgctga ttgcctcgct gctgtcgatt tttcttgcac
    10921 tggtcatggc actgtatttc gcccgccgtt tcgtcgaacc cgtcctatcg cttgccgagg
    10981 gggcaaaggc ggtggcgcaa ggcgatttca gccagacgcg ccccgtgttg cgcaacgacg
    11041 agttcggacg cttgaccaag ttgttcaacc acatgaccga gcagctttcc atcgccaaag
    11101 aagcagacga gcgcaacagg aggcgcgaag aagccgccag acattatctt gaatgcgtgt
    11161 tggaggggct gaccacgggc gtggtggtgt ttgacgaaca aggccgtctg aaaaccttca
    11221 acaaagcggc ggaacagatt ttggggatgc cgcttacccc cctgtggggc agcagccggc
    11281 acggttggca cggcgtttcg gcgcagcagt ccctgcttgc cgaagtgttt gccgccatcg
    11341 gcgcggcggc aggtacggac aaaccggtcc atgtgaaata tgccgcgccg gacgatgcca
    11401 aaatcctgct gggcaaggca accgtcctgc ccgaagacaa cggcaacggc gtggtaatgg
    11461 tgattgacga catcaccgtt ttgatacacg cgcaaaaaga agccgcgtgg ggcgaagtgg
    11521 ccaaacggct ggcacacgaa atccgcaatc cgctcacgcc catccagctt tccgccgaac
    11581 ggctggcgtg gaaattgggc gggaagctgg atgagcagga tgcgcaaatc ctgacgcgtt
    11641 cgaccgacac catcgtcaaa caggtggcgg cattgaagga aatggtcgaa gcattccgca
    11701 attatgcgcg ttcctcttcg ctcaaattgg aaaatcagga tttgaacgcc ttaatcggcg
    11761 atgtgttggc attgtatgaa gccggtccgt gccggtttgc ggcggagctt gccggcgaac
    11821 cgctgatggt ggcggcggat acgaccgcca tgcggcaggt gctgcacaat attttcaaaa
    11881 atgccgccga agcggcggaa gaagccgatg tacccgaagt cagggtaaaa tcggaagcgg
    11941 ggcgggacgg tcggattgtc ctgacggttt gcgacaacgg caaagggttc ggcagggaaa
    12001 tgctgcacaa cgccttcgag ccgtatgtaa cggacaaacc ggcgggaacg ggattgggtc
    12061 tgcctgtggt gaaaaaaatc attgaagaac acggcggccg catcagcctg agcaatcagg
    12121 atgcgggcgg cgcgtgtgtc agaatcatct tgccaaaaac ggtagaaact tatgcgtagc
    12181 agcgatattt taattgtaga cgacgaaatc ggcatccgcg acctgctgtc ggaaatcctg
    12241 caggacgaag gttattcggt cgcattggcg gaaaacgccg aagaggcgcg caagctgcgc
    12301 catcaggcgc gccccgcgat ggtgctgctg gatatttgga tgcctgattg cgacggcatc
    12361 acccttttga aggagtgggc gaaaaacggg cagctcaata tgccggtggt gatgatgagc
    12421 gggcatgcca gcatcgacac agcagtggaa gccaccaaaa tcggcgcgct cgactttttg
    12481 gaaaaaccga tttccctgca aaagctgctg tccacggttg aaaacgcgtt gaagtacggt
    12541 gcggcgcaaa ccgaaacggg gcctgtattc gacaagctgg gcaacagtgc cgcgattcag
    12601 gagatgaact gcgaagtcga agcggcggca aagcgcacgt cccctgtatt tttgacgggc
    12661 gaagcgggtt cgccgtttga aaccgtggcg cgctatttcc ataaaaacgg tacgccgtgg
    12721 gtcagtccgg caagggtcga atatctgatc gatatgccga tggaactgtt gcagaaggcg
    12781 gagggcggcg ttttgtatgt cggcgacatc gcccagtaca gccgcaacat ccaagccggt
    12841 attgccttta ttgtcggaaa ggcggaacac cgccgcgtca gggtggtcgc atcgggcagc
    12901 agggcggcag gttcagacgg cattgcctgc gaggaaaagc tggcggaact gctgtcggaa
    12961 tcggtcgtcc gtattccgcc gctgcgtatg cagcatgaag acattccctt cctgatacag
    13021 gggattgcct gcaatgtggc ggaaagccaa aagattgcgc ctgcctcatt cagtgaagag
    13081 gcacttgccg cattgacccg ttacgactgg ccgggaaatt tcgaccaact gcaaagcgtc
    13141 gttgcaacgc tgttgttgga ggcggacgga caggaaatcg gcgcaggggc ggtttcttcc
    13201 cttttggggc agaatgtgcc tgccgaggga gcggaagata tggtgggcgg gtttaatttc
    13261 aacctgcccc tgcgcgaatt gagggaggag gtggagcggc gttatttcga gtaccacatc
    13321 gcccaagaag gtcagaatat gagcaaagtg gcgcagaaag tcggtttgga acgcacgcac
    13381 ctttaccgca aactcaaaca gctcggcatc ggcgtttcgc gccgggcggg ggaaaaaacc
    13441 gaagaatagg cccggacggc cggttttccg gctgcgggct tttgttttca gacggcattt
    13501 ggtgcaaatg ccgtctgaaa tcgtaagggg acggatttta t
//
LOCUS       DAWHRA010000047        13395 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWHRA010000047 DAWHRA010000000
VERSION     DAWHRA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.047"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(117..545)
                     /locus_tag="WIB30_001007"
     CDS             complement(117..545)
                     /locus_tag="WIB30_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ3797089.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(610..2358)
                     /locus_tag="WIB30_001008"
     CDS             complement(610..2358)
                     /locus_tag="WIB30_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ3797090.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2685..4118
                     /gene="ccoN"
                     /locus_tag="WIB30_001009"
     CDS             2685..4118
                     /gene="ccoN"
                     /locus_tag="WIB30_001009"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ3797091.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4145..4756
                     /gene="ccoO"
                     /locus_tag="WIB30_001010"
     CDS             4145..4756
                     /gene="ccoO"
                     /locus_tag="WIB30_001010"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ3797092.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4761..4931
                     /locus_tag="WIB30_001011"
     CDS             4761..4931
                     /locus_tag="WIB30_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ3797093.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4955..6052
                     /gene="ccoP"
                     /locus_tag="WIB30_001012"
     CDS             4955..6052
                     /gene="ccoP"
                     /locus_tag="WIB30_001012"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ3797094.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAVSEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6092..6286
                     /locus_tag="WIB30_001013"
     CDS             6092..6286
                     /locus_tag="WIB30_001013"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ3797095.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6488..7897
                     /locus_tag="WIB30_001014"
     CDS             6488..7897
                     /locus_tag="WIB30_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ3797096.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYTMDEIHSDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7999..11208
                     /gene="recC"
                     /locus_tag="WIB30_001015"
     CDS             7999..11208
                     /gene="recC"
                     /locus_tag="WIB30_001015"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ3797097.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LDDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSEMAGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPKGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLTDIVRIWRQPATVGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTFAAAEKKEEAGAA"
     gene            complement(11278..12846)
                     /gene="mtrF"
                     /locus_tag="WIB30_001016"
     CDS             complement(11278..12846)
                     /gene="mtrF"
                     /locus_tag="WIB30_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ3797098.1"
                     /translation="MNQTDTRRGGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIYVVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            13178..13390
                     /locus_tag="WIB30_001017"
     CDS             13178..13390
                     /locus_tag="WIB30_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797099.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3349 a         3236 c         3558 g         3252 t
ORIGIN      
        1 gtctcccact gtaaaaacgg agaaggacag aggacggcag taaggtttgc cgtcctcttt
       61 ttcaagttgc ggtggaatgc cgcttgaagg tgtggtttaa acgtttcggc ttgtaatcag
      121 acgcgggcgt tgtccagccg ctctctgaag ttttccaaat ttacgcctgc cgcttgggcg
      181 cggcggatga tttcgtcaac cggcatacct tctgccgccc ggcggaagcc ccacaggagg
      241 gagcagcgcg taccggtccg gcaataggca aggacgggat attcggcttg tccgatgagt
      301 tggcggaagg tttcgacatc gtgtttttgg atgtcgcgtg cggtaacggg ttggtgatgg
      361 aatccagtaa cgcctgcttg ttccagccac tgtttgattt gggcgaagtc gggttgcgat
      421 tcttcttcgc ggtcggggcg gttgcagatg acggttttga tgcccagttg cgcgatttgt
      481 tccgcatcgg ctttggtcag ttgcggagaa atatagaggt gttcgtcaag tttcagaatg
      541 gccattttgt ttcctttact ttgcggttgt ttttcagacg gcattttgtg tgcaatgccg
      601 tctgaaagtt taaccgaaca accagttgtc caacaggttc cggtctgtga tatgtaggat
      661 ggcagtatcc gctgccgctg cctgaaccgt caagacggtt tgacgcaata tgccggcacg
      721 gaagaagtgg atattgattt ttgcattgac gtggtatcgg gcccattgtg cggtaaagtc
      781 ggtgcaggca taaccgtcta aagcaatgat tttgtcttgc gggcacagtg ccgcagattc
      841 cgcgctgccg ccgttgaaga catgggtcag gacgatgtgg tcggtgtttt gtttgaaacg
      901 tgcgccaaaa tcgcctgccg acgggacggg gcagatgtgt tctgcgtatc cgccgccgtg
      961 ttgtcgggga agcggcagga aggtcagtcc cacgcctgcg gttgccaggc attcggcaag
     1021 cggcaaatct tcggtactgt ataacgcttt ttggaaaaaa tcttccaaat ccaagccggt
     1081 aatttcctga cagcggattt gccagtgttt ttccggaata cccgagtgtg tgtccctcca
     1141 ctcccgatag agtttgtcca ttaacgtatc gagagaatgt ctgccgttgc ttcggttgcg
     1201 tattatcaga tcaaggcaca atgcggcaag cgcgcctttc tggtagtagc tgacgatggc
     1261 gttggggctg ttttcatccg gtttgtaaaa tttgttccac gcggtaaaac tcgattccgc
     1321 caaggtctgc ctcaaacggc cgcgggtttg ttgtacgcgc gtaatgcctt gtgccagcag
     1381 gtttaaataa gattcgggcg agatggtgcg gctgcgtgcc aaaaacaaat cgtcgtaata
     1441 ggatgtaata ccttcgaatg cccatagttg ttcggtatag ttttctttgt cgaggtcata
     1501 agggacgaac gcggcaggtt tgatggattt gacgttccac gcgtgaaaat attcgtggga
     1561 gaaaagtccg agcaatgtgg tgtaggtatc gtcggcatcg gtcataccgt acggcggaag
     1621 gctgtggcgg tcggcgagca gggcggtgct gtcggtgtgt tccaaaccgc cgtaaatatg
     1681 gtcgccgacg tggagcagga acaaatattt ttgaaacggg gcaggggagg aaaacaccgc
     1741 cagttctgtt tcgcagattt ttttgatatc cgaaaccagc ctgttgcggt cgaaatcggg
     1801 atatatgccg cttaaggcaa ttgtgtgcgg aatgcctgcc gcctcaaaat ctaaaaattc
     1861 aatcaagccc atctcgacag gtcggtcaat caattcggca taagatgccg cctgaaagac
     1921 aaacctccct gtttccggca gcgttgtggc aatacgccat tcggacggaa tacccgtcaa
     1981 ttccaagcgg tgcggcagcg tttccgttcc ttcgactttc aaaaacaggc acgatccgtc
     2041 aaaaaaaccg cgttctgtcg tcaggaaaga acctcgaacc gacaaatcga atgcatatac
     2101 ggtgtagcgg atttgccact cgccgcgtac ggcggcggca tgccagcggt ttttggcaat
     2161 ttgttcgagc ggcatggacg tgccgttaca ggatgcatgg atagaagtga tgtggcggga
     2221 aaaatcccga atcagatagc tgcccggaac ccagtttggc aggcttattt ctataggaag
     2281 atcatcatct tgtgtgaatg tcagcaggat gtgccactcg tggtcgagag gggacggggt
     2341 tattttataa tgaatcataa ctatcaaata tatcgttaaa cgggatggat taaatacctt
     2401 taaaatgcag cttaacctac atcaataaag tgccacaaaa agggaaaatt ggaaacaaaa
     2461 gcaatggcgg tcgggttgta atttattata acaatatgat ttatatgtaa taaaaaacag
     2521 attaagcaaa gggctcgtcg gcggcgcgaa tgtaattccg cttggcgtaa taaggcagat
     2581 atgttaaatt tagaaaaaat tacgcttgcc cggattgcac cttaaacagg ctgcggaagc
     2641 ggcaggataa aaatctttat tcccataaat tcatggagac tctcatggac acacaaactt
     2701 acaactacaa agtggtgcgc cagttcgcca tcatgactgt agtttggggg attgtgggta
     2761 tgttggtcgg cgttatcgtc gccgcccagc tttttgctcc tgccctcgac ttgtctaata
     2821 tcggaccttg gttccacttc ggccgcctgc gtccgctgca caccaatgcg gttattttcg
     2881 catttggcgg ttgcggcctg atcggcacat catactacgt tgtccaacgt acttgtaata
     2941 cccgtctttt cggcggttgg ctgccggcat ttaccttttg gggctggcag gcggtaatcg
     3001 ttgccgccgt cgtcagcttc cctatgggtt ggacccaagg taaggaatat gccgaactgg
     3061 aatggccgat cgatattctg attactttgg tttgggtggc ttacgccatc gtattcttcg
     3121 gtacgattgc caaacgtaag attaaacata tttacgttgc caactggttc tacggcggct
     3181 ttattttggc ggtcgcactt ttacacatcg tcaacaatat cagcatcccg gccggtttga
     3241 tgaagtcata ccccgtctat tccggtgcga ttgatgctat ggttcaatgg tggtacgggc
     3301 ataatgccgt gggcttcttc ctgactgccg gcttcttggg tatgatgtac tatttcgtac
     3361 ccaaacaagc agcccgcccc gtttactcct accgcctgtc cgtcgttcac ttctgggcgt
     3421 tgatttttac ctatatgtgg gcgggtccgc accatcttca ctacactgcg ctgcctgact
     3481 ggacgcaatc tttgggtatg gttctgtctt tgattctgtt cgcaccctct tggggcggta
     3541 tgattaacgg catcatgacc ttgtccggcg cgtgggacaa actgcgtaca gacccgattc
     3601 ttaaattcct gattgtatcc ttgtccttct acggtatgtc tacctttgaa ggcccgatga
     3661 tgtcgattaa aacggtcaat gcattgagcc actatacgga ctggaccgtc gcgcacgttc
     3721 atgcgggtgc gttgggctgg gtaggctttg taaccatcgg ttccgtctat tacatgattc
     3781 cccgtctgtt cggcaaagaa cagatgcaca gcaccaagct ggtagaagca catttttgga
     3841 ttgcgaccat cggcgtggtt ctgtatatcg ctgccatgtg gattgccggt gtgatgcagg
     3901 gtttgatgtg gagttctttg aacgatgacg gtacgctgac ctactcgttt gtcgaatccg
     3961 taaaacgcac catgccttac tacgtgatcc gttttgcagg tggtttattg tatctgagtg
     4021 gtatgtgcat tatggcgtac aacgtgtacc gcacagccat cggtggtaaa gcagtcgatg
     4081 ccgaaatccc tgcggtttcc caaacacagc accactaaaa tactaagaaa gataggctac
     4141 caaaatgaaa ttacaacaat tggctgaaga aaaaatcggc gttctgattg tgttcacgct
     4201 gcttgtagtc agtgtcggtc tgttgattga agttgtgccc ttggccttta ccaaggcggc
     4261 aacacagccg gcgccgggcg tgaagcctta caatgccctg caggttgccg gacgcgatat
     4321 ttacatccgt gagggctgtt acaactgcca ctcgcaaatg attcgtccgt tccgtgcgga
     4381 aaccgagcgt tacggtcatt actctgttgc cggagagtcg gtttacgacc atccgttcca
     4441 atggggttcc aaacgtaccg gtcctgattt ggcacgtgtg ggcggtcgct attccgacga
     4501 atggcaccgt atccacctgc tgaatccccg tgatgtcgtg cctgagtcca atatgccggc
     4561 attcccgtgg cttgcacgca ataaagtcga tgtcgatgca accgttgcca acatgaaggc
     4621 tttgcgtaaa gtaggtactc cttacagtga tgaggaaatt gcgaaagcac ctgaggcttt
     4681 ggcaaacaaa tccgagctgg atgctgtagt cgcctatctg caaggattgg gtctggcttt
     4741 gaaaaacgta aggtaacatc atggatatta acggtattcg tgctctcttc acggtatgga
     4801 tctttatctg tttcctgttg gtactctata tcgtcttcaa caggcggaat aagaaaaact
     4861 acgataatgc cgcaaacagc atttttgatg aaaaccaaga tgcgcaagat aagaaaagcg
     4921 aaaaccgtta atattgtgat aacggagcaa aacaatgaac acaacatccc aatttaccag
     4981 taatttctgg aatatatata ttgcagttat tgtcttactg agctttatcg ctttggcttg
     5041 gctgctgctg tctcaaaatg ttgtcaaacg tccgaagaag ggcgaagaag tacaaactac
     5101 gggtcatgag tgggacggca ttgccgaata cgacaatccg ctgccccgct ggtggttttg
     5161 gctgtgtgtt ttgacgtggc tgttcggtat cggttatttg gttatgtatc cgggtgtcgg
     5221 cgactacaaa ggtctgctga aatggaccag ccataaccaa tatgaaaaag aggtcaaaaa
     5281 agccgatgag caatacggca aactgtatgc caagtttgcg gatatgccga ttgaaaaagt
     5341 ggcaaaagac cctcaagcca agcaaatcgc ccaaaacctg tttaacactt attgtatcca
     5401 gtgccacggc tctgatgcta aaggctctaa aggttttccg aatctgaccg atagcgattg
     5461 gttgtggggc ggtgatcccg ataaaatcca cgagaccatc gaaaaaggcc gtgttgcgac
     5521 tatgcctgcc tggggtcctg ctttgggcga agaaggcgtg aaagacgttg cccattatgt
     5581 gatgtccctt tcaaaacccg aaggtcagta tgatgaggaa cgtgccgcgc gcggacaagc
     5641 cttgttcagc ggtccgcctg ccaactgttt cacttgccac ggcgataagg gacaaggtat
     5701 ccaaggtttg ggtccgaacc tgactgatga cgtgtggttg tggggcggta cgcaaaaatc
     5761 cattatcgaa accattacca acggtcgcag cagccaaatg cccgcttggg gacatttctt
     5821 ggataaagac aaactgcata ttatgactgc ttatgtatgg ggtctttccg ataaagacgg
     5881 taaagctccg gtgaaaaaag ccgagcctgc accgactccc gcaccggcgg cagaacccgc
     5941 agcctctgct cctgcagaag cagcacaagc cgtgtccgaa gccaaacctg ccgcagcaga
     6001 acctaaagcc gaggaaaaag ctgcacctgc tgccaaagcg gacggcaaat aggtttatga
     6061 aaccgtttgt gccgcctgcc atggcaatgc gattccgggt attccccatg taggcatcaa
     6121 agccgattgg gccgaccgca tcaaaaaagg caaggacacg ttgcacaaac acgcgatcga
     6181 aggtttcaat acgatgcccg caaaaggcgg tcgcggcgat ttgagcgatg atgaggttaa
     6241 agctgcggtt gactatatgg tcaaccagtc aggcggcaaa ttctaacttg actgagtttc
     6301 cgatattatc ggttatgaaa atccagattc agtattgaat ctggattttt tgttttaacg
     6361 ggcatccggt tttaaacgca gactgcctat ttgttgtaat gaattttgac tttagtcata
     6421 aagaattagt ctttattgtt tataatcgcg catcatcacg atagccctat tttcaaaagg
     6481 acgattaatg gatacacaaa tcaaaactga agccgacaat cagagcaacc ggcgttatct
     6541 gaccgtttgg cggtggcatt tttatgccgg tctgctggtt atgccttttc ttaccctgct
     6601 tgccgttacg ggtttgggta tgctgctgtt tgccaatatt accggtaagg agggcgagcg
     6661 gattcatgtt gtgccgcagg caacggtaca acctctgtct gttcaggcgg aagcggcacg
     6721 cagtgccgtt aatccggaaa cttcgtccgt cgttcagtat attgcgccgc gtgccgatga
     6781 tatggttgcc gtgttccgtg tcaacaatga gggcaaagca acgatggtcg cggtcgatcc
     6841 ttatacggca aaagtggtca gtaccatgcc gcgcaatcag ggttggtatt acacgatgga
     6901 tgaaatccac agcgatatga tgctcggtgc ggcaggcgat tatcttttgg aaacggcagc
     6961 ttcactgacc attattatgg ttgtcagcgg cttgtacctt tggtgggtga aacggcgcgg
     7021 catcaaggcg atgctgctgc cgtcaaaagg cagggcgcgt tcttggtggc ggaatctgca
     7081 cggcacgttt ggaacttggg tgtcgttgat tttgctgttg ttctgcctgt cgggtattgc
     7141 ttgggcgggt atttggggcg gcaagttcgt acaggcttgg agtcagttcc ctgccggtaa
     7201 atggggtgtc gaaccgaacc ccgtttcagt cgtgccgacc cacggcgagg tattgaatga
     7261 cggcaaggtt aaggaagtgc cgtgggtttt ggagcttacg cctatgcctg tttcagggac
     7321 gactgtgggc aaagacggca ttaaccctga cgagccgatg acattggaaa ccgtcgaccg
     7381 ctttgcgcgg gaaatcggtt tcaaagggcg ttatcagttg aatttgccca aaggcgagga
     7441 cggcgtatgg actttgtcgc aggattctat gagttacgac atgatcagcc cgtttgccga
     7501 ccgcacggta catatcgacc agtacagcgg caaaatcctt gccgacatcc gttttgacga
     7561 ttacaacccg ttcggcaaat ttatggcggc aagcattgcg ctgcatatgg ggactctggg
     7621 ctggtggagc gtgttggcga acgtcttgtt ctgccttgcc gtcattttta tcggtatcag
     7681 cggctgcgtg atgtggtgga aacgccgtcc gaccggagcg gtgggcatcg ttccgccggc
     7741 gcagaaagtc aagctgccgg tttggtggat gatggcattg ccgctattgg caatcgcact
     7801 gctcttcccg accgcactgc ttgccattgc cgtgatttgg ctgttggata cgctgctgtt
     7861 gtcgcggatt cctgttttga ggagatggtt taaatgacca aatgccgtct gaaaggttca
     7921 gacggcattt tgtttgaagg cggacggggg aaaggctata taatcccgaa tacttgacca
     7981 cagcttctgt ttgaaatcat gttttatctg tatcaatcca accgtcttga aacgctggcg
     8041 gcattgtttg cccgcattca aaaagtcaaa ccgctgaaat cggctttaca gcccgaacag
     8101 attattgtgc agagtcaggg gatgcgccgc tacctcaata cctgcctcgc ccgcgatttg
     8161 ggcgtggcgg cgaatttgtc gttcagcctg cccgccggcc tgacgtggaa gctgatgaaa
     8221 aaactgattc ccggtattcc ggaactcagc ccgtttgcgc ccgaagtcat gcgctggcgg
     8281 ctgctggatt tgttccgcag cgaggcattt cggaatacgg cagaatttga agatgtgagg
     8341 aatgtgctgc aagactatct gggcagcggc gaatcggcag attaccagct tgcgggacag
     8401 cttgcggaca tattcgacca atacctcgtg taccgtcctc agtggataga cgcttggcag
     8461 cagggcaggc ggctcggttt ggatgacgac gaaatctggc agtccaaact gtggcgttac
     8521 ctcgacgacg gcaggcagag cgcgccgcac cgtgtcgcgt tgtgggaaaa gctgttggaa
     8581 tctttgagca gtgataagct gcccgagcgt tatttcgtgt tcggcatttc cacgatggcg
     8641 ccgatgtatt tgcaactttt gcacaagctg tccgaacatt gcgacgtgtt cgtgttcgca
     8701 ctcaatccga gcgggatgta ctggggcaac gtcatcgaag cggcgcaaat cctcaaaggt
     8761 ggcggcgatc ccgatttaac tcaggcaggg catccgctgc tcgcttcatt gggcaagcag
     8821 gggcgggact ttttcgactt tttgaacgaa atggaaatag aaggcgaaac gccggtattt
     8881 gaggaaggcg ggcgcgatac gcttttacac gccctgcaaa ccgatattca aaacctgaaa
     8941 atgccgtctg aaatggcggg aagcgtcaac acgggcgacg gctcgatacg catcgtctcc
     9001 gcacacagcc ctttgcgcga attgcagata ctcaaagaca agctgttgaa aattctgcat
     9061 gaacatcccg attggcagcc gcacgatatc gccgtattaa ccccgaatat cgaatcctat
     9121 acgcctttta tcgaagccgt gttcggacag gcgcagcccg gtgcgcaggc attgccgtat
     9181 tccgtgtcag acgtgaaaat cagccgccgc caaccgctgt ttcatgcttt gtcatgcctg
     9241 ttcgacttgt tggaaagccg atttgaagtc gataaagtgc ttgtgctttt agaaaccgcg
     9301 cccgtgttgc gccgtttcgg actgactgag gacgatttac cgcttttgca cgacatggtt
     9361 gccgatttga acgtccactg gggtttggac ggagaaatgc gcggcggcac ggatcagctg
     9421 ttcacctgga agcaggcggt agaacgcatg atattgggct ggatgctgcc caaaggcggc
     9481 aatccgatgt ggcaggatgt cagcgcgtgg tatgccgacg tgaatcaaac cgccatgttc
     9541 ggacgttttg ccgccttcct cgaaaccctg acggatattg tacggatatg gcggcagccc
     9601 gcaacggtcg gcgaatgggt ggcgcgttgc cgggatttgc ttgaaacatt gttccaagct
     9661 gaagccgatg accaaaagtc agtccaaaac cttgaaaacg aatgggtcaa atggcaggcg
     9721 gaaaccgaat tggcgcaatt ttccggacag ttgccgccgc acaccgtcat ccgccatatc
     9781 cgacgctttc tcgacagcga aagcgaggca ggctttttac gcggcggcat caccttttgc
     9841 agtatggtgc cgatgcggag cctgccgttc aaagtcatct gcctgttggg tttgaacgac
     9901 ggagattttc cccgtaatac caaagccgcc gtattcgacc tgattgccaa acatcccgcc
     9961 aaaggagacc gcgcccgccg cgatgacgac cgctacctgt tcctcgaagc cctcatcagc
    10021 gcgcgtgaaa tcctctacct gtcctacatc gggcgcgaca tccgcaaaga cgaagagctt
    10081 gccccgtctt ccctgttggg cgaactcatc gataccgttg ccgctatggc gggcatcggt
    10141 agccgccaac ttgcacaaaa cctgatagaa cagcatccgc tgcaagcctt ctcgcgccga
    10201 tattttcaag aaggcggacg ttcagacggc atattcggca cgcgtaccga ctacgccgcc
    10261 gcgctcggac aaacgcccga accgccgcaa ccctttttcg atcaacccgt agaaaacgcc
    10321 gaacctgttg ccgaaatcgg acaggacgaa tttatccgct tctggcgcaa ccctgtcaaa
    10381 gtatggcttc agcagcagct tgcgtggagc gaaccccata tcggcgaagc ctgggagcct
    10441 gccgaaccct tcgagccgca acacgccgat caaatcgccg aaatctacat cgaagcacgg
    10501 cgcgaaggac gggattttgc ccaaaccgcc gcccgcatcg gggcggaaag cctcctgccg
    10561 tcgggagagt tgggcagact ttggcagcag gacttccaaa ctgctgccaa acaaatcgac
    10621 acggcggttt taaacagccc caaactgcca ccgctttcat atgccatacc gtcggacggg
    10681 caaatcctga aaggcagctt gggcaatctg taccgctgcg gacaagtgtt ttacgcctac
    10741 ggcaaaccca acgcgccgca acgtatcgct tttctgctgg aacacctgat attttgcgcc
    10801 gttatgccgt ctgaagccga aacgcggcaa acctttatcg tccaatccgg agaaaccgaa
    10861 atattggcgg aaatcgcgca agacagggca ttacagctat tgtcggaatg gatggcgttt
    10921 ttcaatatcg ggcaaaaccg cccgctgccg ttttttgcca aaacctcgct tgccaccgcc
    10981 gaagcgtttg cccaaaaaca agattgggaa gccgccctga aaaaagccca aaccgcctac
    11041 cacggcaaca aagtcagcaa agggcagaaa gactataccg aagtcgccct cgtgttcggc
    11101 aacgcaagcc aaaacccgct cgaacagccc ctgtttgaaa acctcgtccg tctgcttgcc
    11161 gacacgtttg ccgcagcgga aaaaaaggaa gaggccggag cagcctgacg ggatggaaag
    11221 gcaaatgccg tctgaaaaca gcaaaaaggt tttcagacgg cattttgttt atcgtgttta
    11281 aggtgcggga tacaaagtgg gtgcgccggg accgacgggc agacccaaaa caaataccca
    11341 aatgcagaat aaggcaatcc atgcaattaa gaagaaagcg gaatacggca acatcataga
    11401 aatcagcgta cccacgcccg catctttttt gtatttgatc accgtcgcca taatcagccc
    11461 gaaataactc atcatcggcg taataatatt ggtaacggaa tcaccgatgc ggtaagcggc
    11521 ttgaatgact tcgggcgcgt agccggccaa catcagcata gggacgaaaa tcggcgcagt
    11581 taccgcccat tgcgcggagg cggagcctat catcagattg ataaaagcac aaattaaaat
    11641 aaaaccgata aacaacacgc tgccgcccaa gccgacttct tttaagaacg tcgccccttt
    11701 aacggcaata tattgcccaa tattcgtcca attaaaaaat gcgacaaact gtgcggcaaa
    11761 aaagatgatg accaaataaa gccccagagt actcatcgat tcggccatcg cattaacgac
    11821 ttcctgttcg ccgcgcaaac ttcgggttac ccggccataa acaatgcccg gcagcgcaaa
    11881 caacaagaaa ataaaaacaa caatcgattt taaaaacggc gaaccggaaa ccaatcctgt
    11941 ttcaggatga cgcaaaatac cgtcggcagg gacgatgctc caagccaata gggcggataa
    12001 ggcaacaaac accacgccag cccaaattaa tcctttatat tccaaaggcg tgatttcatt
    12061 ggaatgccga atgtcttttt cttcttgtga caaatctgat tgataagggc ccaattgcgg
    12121 ttcgacgatt ttttcagtaa caaaataacc aatcaaagca atcacaaacg tactggctac
    12181 cataaaaaac cagttggctt cagggcctac gacgtagtcg ggatggatga tttgcgccgc
    12241 ctgttgggtg atgcctgcca agagcggatc gattgtgcct aagaacagat tggccgaata
    12301 accgcccgaa acgccggcga aagccgcagc cagaccggca agcggatggc ggccgaggga
    12361 atgaaagatg atggcggaca aagggattag gacgacatag cccaattcag aagcggtatt
    12421 agataaaatc cctgtaaaaa caaccataaa agtagtgagt ttgcgtggag attttgtgag
    12481 caataagcgc attaatgcgg aaatcaagcc cgatttttcc gcaatcccca cgcccaataa
    12541 agaaaccaac accgttccca acggcgcgaa accggtgaaa tttttaacgg tatgcgtcag
    12601 gattttgatc aaaccgtcgg cattgagcag gctgacaacg taaatcaaac cgtcatcggc
    12661 acgtcctttc gcaccaacag ggcgcggatc ggggacggat agtccgaaat acgcaccggc
    12721 ggcagaggca atcagcaata acacaatgaa aataataaaa agcgtaaccg ggtgcggcaa
    12781 catattgccc agccattcga ctgtgcgtaa aaatcgtccg ccccgccgcg tatcggtttg
    12841 attcatctgt ttctccttaa aaaaatatat cgtgtgcatt aaatttttga atgtaacatg
    12901 aagtgttttg taagcaaact aaaataatcg catatgtata taaccgcaat agattaaaac
    12961 agaaaatgcc ctcatcgtta ttatcgtaaa ggcgggaatc cgaaagctcg acgttaaagc
    13021 cttatcagga atgactggaa ctcaaaaaac ggtttcccgc tttcgcggaa atggcgggat
    13081 tttcggtttt tatttgcaaa aatgccggag aagaatgcag tttacatttt gaatggtatt
    13141 gttattaatt gtaaataaat attggaaggt aaatgatatg aaaaatgaaa ttcaaaaaat
    13201 catggacaaa tataatccat ggcatgaaga tgattttgag tcgtatgaag acattgccaa
    13261 agatgtatcg ctgatgacag ataaaacgtt cattgaacat tatttgttag aagtttattc
    13321 agaagaaaac ggacattttg accaagagaa tgtccatgca atgatagaag aaattaaaaa
    13381 tgcaatttag tgaat
//
LOCUS       DAWHRA010000048        13353 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWHRA010000048 DAWHRA010000000
VERSION     DAWHRA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13353
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.048"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(78..278)
                     /locus_tag="WIB30_001018"
     CDS             complement(78..278)
                     /locus_tag="WIB30_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797100.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            421..1560
                     /locus_tag="WIB30_001019"
     CDS             421..1560
                     /locus_tag="WIB30_001019"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ3797101.1"
                     /translation="MSQNASVDIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            1557..2138
                     /gene="metW"
                     /locus_tag="WIB30_001020"
     CDS             1557..2138
                     /gene="metW"
                     /locus_tag="WIB30_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ3797102.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRILERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            2255..3100
                     /locus_tag="WIB30_001021"
     CDS             2255..3100
                     /locus_tag="WIB30_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ3797103.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(3150..3710)
                     /gene="efp"
                     /locus_tag="WIB30_001022"
     CDS             complement(3150..3710)
                     /gene="efp"
                     /locus_tag="WIB30_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ3797104.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(3753..4901)
                     /gene="earP"
                     /locus_tag="WIB30_001023"
     CDS             complement(3753..4901)
                     /gene="earP"
                     /locus_tag="WIB30_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ3797105.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGALRKRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPLTLLLAGAQIIDS
                     LKQNGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETASAHRCLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(4920..5198)
                     /locus_tag="WIB30_001024"
     CDS             complement(4920..5198)
                     /locus_tag="WIB30_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797106.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIVLLVVYLFHYFGAF"
     gene            5378..6319
                     /gene="miaA"
                     /locus_tag="WIB30_001025"
     CDS             5378..6319
                     /gene="miaA"
                     /locus_tag="WIB30_001025"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ3797107.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            6323..7042
                     /locus_tag="WIB30_001026"
     CDS             6323..7042
                     /locus_tag="WIB30_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ3797108.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSCHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLNRFFQNKRKG"
     gene            7047..7535
                     /locus_tag="WIB30_001027"
     CDS             7047..7535
                     /locus_tag="WIB30_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ3797109.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALVGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTTFKIGCAVSILFILLVKYIAY
                     LF"
     gene            7601..8353
                     /gene="rlmB"
                     /locus_tag="WIB30_001028"
     CDS             7601..8353
                     /gene="rlmB"
                     /locus_tag="WIB30_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ3797110.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(8415..9806)
                     /locus_tag="WIB30_001029"
     CDS             complement(8415..9806)
                     /locus_tag="WIB30_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ3797111.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASPVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(9821..10075)
                     /locus_tag="WIB30_001030"
     CDS             complement(9821..10075)
                     /locus_tag="WIB30_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797112.1"
                     /translation="MLPIVAYFTAGVKSGGSGMHGAAWRRMRRVCIFVFEIRLLRFAN
                     DIPAATVRNLFAGRARMPSEIEIEAVWRIRYNPRFGFFNI"
     gene            10113..10988
                     /locus_tag="WIB30_001031"
     CDS             10113..10988
                     /locus_tag="WIB30_001031"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ3797113.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQVDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEN
                     GQAKVRAALKASGQL"
     gene            10997..12193
                     /gene="bamC"
                     /locus_tag="WIB30_001032"
     CDS             10997..12193
                     /gene="bamC"
                     /locus_tag="WIB30_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ3797114.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDKVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTVLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(12250..13182)
                     /gene="pip"
                     /locus_tag="WIB30_001033"
     CDS             complement(12250..13182)
                     /gene="pip"
                     /locus_tag="WIB30_001033"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ3797115.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIRKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEDVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT         3413 a         4018 c         3274 g         2648 t
ORIGIN      
        1 cacctcctac cgcaagccct tttcatcaaa tatgccgtct gaaacagaaa aatgcgtttc
       61 aggcggcatt ttttccgtca atacgaatcc ggaatgtgtt tgaaatacaa cataaacaga
      121 taccaaaaca gcgtcgcggc ggccgcaccg agcagcaccc gggcactgat ttcggcaact
      181 tcgcgcaggg cgcgtttctg cctgcggctg aagaggcggt tgataatcag cgcggcaagt
      241 gcgaaaaaga aaaaaatacg gaaaagtctg cctatcatat cgggaagcga aaagtatggt
      301 tcgggcgcgg cagcatggga aaggatattt tagcgcatcg ggcttcttgt ccgaccgtgt
      361 caaaaagtca tgccgtttgc gatataataa cgcttttcgc cgcatttgtt ggaacaggat
      421 atgagtcaaa atgcctcggt ggacattgta acgccccaaa aaattccgtt tgagatgccg
      481 ctggttttgg aaaacggtaa aactttgccg cgtttcgatc tgatgattga aacctacggc
      541 gagctgaatg ccgaaaaaaa caatgcggtt ttaatctgcc acgcgctgtc gggcaaccat
      601 catgttgcgg gcaggcattc cgcagaggat aaatatacgg gctggtggga caatatggtc
      661 ggacccggca aaccgattga tacggaacgt tttttcgtgg tcggtttgaa taatctgggc
      721 ggctgcgacg gcagcagcgg gcctttgtcg attaatcctg aaacgggtag agaatacggc
      781 gcggattttc cggtggttac ggtgaaggac tgggtaaaat cacaagccgc gcttgccgat
      841 tatctcggca tcgggcaatg ggcggcggtt gtcggcggca gcttgggcgg catgcaggct
      901 ttgcagtggg cgatttccta tcccgaacgc gtgcgccatg ctttagtgat tgcgtctgcg
      961 ccgaagctgt ccacgcaaaa tatcgcgttc aacgatgtgg cgcgtcaggc gattttgacc
     1021 gaccccgatt tcaacgaagg acattaccgc agccgtaaca ccgttcctgc acggggctta
     1081 cggattgccc gcatgatggg acacattacc tatcttgccg aagacggttt gggcaaaaaa
     1141 ttcggacgcg atttgcgctc caatggctat caatacggct atggcgttga atttgaagta
     1201 gaatcctatc tgcgctatca aggcgacaaa ttcgtcggac ggtttgatgc caacacctac
     1261 ctgctgatga ccaaagcttt ggactatttc gatccggcgg cggatttcgg caacagcctg
     1321 acccgcgccg tgcaggatgt gcaggcaaaa ttctttgtcg ccagcttcag caccgattgg
     1381 cgtttcgcgc ccgaacgttc gcacgaactg gtcaaggcac tgattgccgc acaaaaatcc
     1441 gtgcagtata tcgaagtcaa atccgcacac gggcacgatg cctttttaat ggaagacgaa
     1501 gcctatatgc gtgcggtcgc cgcctatatg aacaacgttt ataaggaatg tcagcaatga
     1561 acctgcgcga tgatttgcaa ctgatttacg accggatacc cgaaggcagc cgcgtcttgg
     1621 acttgggctg cggcgacggc gaactgcttg ccgccttggt cgaacacaaa aaatgcagcg
     1681 gctacggcat cgaaatcgac accgacagcg tgattgccgc gatgtcgcgc ggcgtgaatg
     1741 ttatccaagc cgatttggaa gaaggtttaa ccgcattcaa cgatcaaagt tttgatgtga
     1801 ttgtgttgag ccaaaccatc caagcgatgc agaataccga aaaaatcctg cgctgcctga
     1861 tgcgcgttgc caaacaggcg attgtcagct tcccgaattt cggctactgg cgcaaccgcg
     1921 tccaaatcgc actcggcgga catatgccgg tttccgaacg tatgccctac cattggtacg
     1981 acacgcccaa tatccattgg tgcacgctca aagactttga tttattgtgc gccaaaaaca
     2041 acatccgcat cctcgagcgc gccgtgatga cgggcaacag acaggtcaaa catttcccca
     2101 accttttggg cagtcttgca ttttaccgcg tcgggtaaga cagattgaga accttgtcgg
     2161 cattaacagg ttcttcggtt ctattaacaa caggccgtct gaaaaatgaa acggtaggtt
     2221 ttcagacggc ctctttaata ctggaaacaa tagaatgaaa aacaaaacct catcacttct
     2281 cttatggctt gccgcaatga tgctgaccgc gtgttccccg agcaaagaag ataaaacgaa
     2341 agaaaacggc gcatccgccg cctcgtccac ggcatccgcc gcttcgtctt ccgcgcccca
     2401 aaccgatttg caaccggccg catccgcccc tgataacgtc aagcaggcag aaagcgtgcc
     2461 gccgtcaaat tgcaccgacc tgcaccccgc caccggcatt gacgatctca tgcagcaaat
     2521 cgccgaacac attgactcgg actgtctgtt tgccctttcc catcacgaac tggaaacccg
     2581 tttcggctta cccggcggcg gctatgacaa catacagcgg ctgctgtttc ccgacatccg
     2641 ccctgaagat cccgactacc atcagaaaat catactggca attgaagact tgcgttacgg
     2701 aaagcgcacg atcagccggc aggcacaaga tgccttgatg gaacaggaac gccgcctccg
     2761 agaagcgacg ctgttgctga tacagggcag tcaagaaacc cgcggacaag gcgaggagcc
     2821 gaaacgcacg cgttattttg aagtttcggc aacccctgcc tattcgagcc ggcacaacaa
     2881 cggacttggc ggcaatttcc aatacatcgg ccaattgccc ggctatctga aaatacacgg
     2941 agaaatgctt gaaaaccaat cactcttccg gctgtccaac cgtgaacgca atcccgacaa
     3001 accgttttta gacatccatt ttgacgaaaa tggcaaaatc acgcgtattg tcgtttacga
     3061 aaaaaacatc tacttcaatc caaacttggg gcgaagataa aaaaatgccg tctgaaacgt
     3121 tttcagacgg catttttcaa tgcggcaaat caagcgcgtt tgcggaattc gccggtgcgg
     3181 gtatcgattt cgattttgtc gccgttttcg atgtaagaca tcacttggat ttcggtgccc
     3241 ccgaccaggc gcgcggtttt catcactttg cctgaagtat cgcctttgac ggcaggctcg
     3301 gtatactcga cttcgcgtac gatgatggtg ggcagttcga cagaaatggg gttgccttcg
     3361 tagaaggtaa cttcgcattg gtcttccata ccgtcaacga tgaatttcaa cgcgtcgccg
     3421 atgttgtcgg cttcgatttc gtattggttg aattcttcat ccataaagac gtacatcggg
     3481 tcggcaaagt agctgtacgt acagtttttg cgggacagga tgaccacgtc gaatttgtcg
     3541 tcggctttgt aaatggtttc ggaagccgcg ccggtcagca ggtttttcag tttcatgctg
     3601 actttggcgg aagagcggcc gcctttgatg tattcggttt tttgaacgac cataggatcg
     3661 ttgccgacca taaatacatt gccggcgcgc agttcttgtg ctgttttcat tgtgattttc
     3721 caatcaaatc ggttgtcgta aaacgcacta ttctagcgta ttttttgatg ctttgaaaca
     3781 aaggcggcga gtttttcgga tgcggaaggc tgcccgaaaa gataacggct ccaatcctcc
     3841 gcgccttgcc gccagccgtt ttgatgttgt tgcaggattt gccaacattc gaggcgttgc
     3901 gtggcggata aagcccctcc gccgtttagg tcgtctgaaa ggcagcggtg tgccgatgcg
     3961 gtttcgggcg tgtagaaacc gtgtgcctta tcccaaaagg cgtggagttt gtcgagatgg
     4021 acattctcgt cttgcggata gatgtgccaa aagaagggtt ttccggcaag ctgggcgcgc
     4081 acgaaactgt cttcgccgcg gatgacggcg cagtcggcaa ggtgcagcag tttgtcgaag
     4141 tcctgttgcg gcacgaaagg gattttgacg aggcggacgg atgccgtctg aaaaacatcg
     4201 ccgtcgtttt gcagggcatc ttgcggaata acgccgtttt gtttgaggct gtcgataatt
     4261 tgcgcccccg ccagcaacag tgtcaacgga ctgcctgcct gtcgccacat ttccagccac
     4321 tttgcccaaa catcgctccg atagccgaaa agcagccatt cgggggcgtt tttttcggga
     4381 agcatcagcc tcttgcgcaa ggctccgcta tcgaaacgga cggcttcgca gtaatcacgt
     4441 tcgcgtatca acccgccgct tttttcgctg aaacccataa accaaaaata tttttgaaca
     4501 ccctcctgcg gcgaaggcat cagatgcagc ctttcattgc tttcctccgc gctcaaatat
     4561 tcccaattca gccaaagcgg cttgtgtcgg cggataatgt gcagcacatt ttcgggcagg
     4621 tcgcaggcaa aagtttcgat gacgacatcg ggaacaggcg cggtatcaat atctgccgca
     4681 tcggaatgcc aagtgcggac atgaatatcc tgatgaacgc agggaacatc gggcaaatca
     4741 gggcaaagcg cacgcaaggc ggacacatcg tccgtccaca aatgcacctg ccaaccgagt
     4801 tcgcggtgca aaacacgggc gagccgccac gaaacgccga tgtcgccgaa attgtcgatg
     4861 accttgcaaa aaatccaaca gacaaaagga ggagtattca tagaaataac ggaaacaagc
     4921 taaaacgcgc cgaaatagtg gaacaaatac acaaccagca aaacaatcgg aataaacagc
     4981 aacatggttt gacgtttcaa atcaaactct tgtttcaaat gatgcttgat ggtgtcgaaa
     5041 aggacgaatt tgaacaaaaa cgtcgcttcg caggcaagga tgatgccgtt gatcagatgc
     5101 tggggtttgt cttcgggcgg attggcgacg aaaacggcaa gtgtcgccaa agggatgagg
     5161 cagacgacaa gccagcggac aagcaggttt cgggacattg caaggctttc aaaaaagcaa
     5221 aacgccatta tcgccccaag cgcacgcctt gtctaacgga cgcgccggcg atgccgtctg
     5281 aagccttgcc gcaggaaaac gcagtgcttc cgcaaagcaa aatataaggg cggcgcgctt
     5341 tccgctataa tccgcatttt cgcaaccctg aaacatcatg ccgaccccga aagcctttgc
     5401 cctgctcggg ccgaccgccg gaggcaaaac cgcgctcgcc ctcaaaattg ccgaaaccct
     5461 gcctgtcgaa atcatcagcc tcgattccgc gctggtttac cgcgatatgg acatcggcac
     5521 ggcgaaaccg tccgcctccg aacgcgcctt tgtcccgcac caccttatcg acatcatccc
     5581 gcctaccgaa tcatacagcg ccgcccgctt tgtcgaagac tgcacgcgct tggtcgggga
     5641 aatttcctca cgcggcaggt ttgcccttat tgtcggcggc acgatgatgt atttccgcgc
     5701 cctgacccaa ggtttgaacg atttgcccga agccgacgcc tgcctgcgtg ccgacttgga
     5761 cgaacaaaaa cagatgtacg gcttggattt cctctaccgc accctgcaaa aagtcgaccc
     5821 tgaaaccgca tgccgtctga aaccgaacga cagccagcgt atcgaacgcg ctttggaagt
     5881 ttattatctg accggcaaac cgatgagcga acacctcggc agacaaagcc cccatacgct
     5941 ccccttcgat ttgcataccg ccgccctgat tcccgaaaac cgcgcccgcc tgcatgaaaa
     6001 catcgccctg cgtttccacc tgatgcttga acagggcttt atcggcgaag tagaaaacct
     6061 gcgccgccgc tatcccggcc tgaccgccga ctcccccgcc atccgctgcg tcggctaccg
     6121 tcaggcatgg aaatatctgg atggaaaaac cgatttcccg gcatttgtcg agaaaggcat
     6181 tgccgccacc cgccaacttg ccaaacgcca actgacttgg ttgcgcaaaa cacctttaga
     6241 ctgcgttgcc gacccgtttt cagacggcac ttcatgcaca cgcctgattg aggcggcaaa
     6301 acggtttttc ggcgcataaa ccatgctgac cacaccgccg ctcgccccca aaaccgtcgc
     6361 cgccctgcac aggctcggta tccgtacgct cgaagaactg cggcaaaacg gttcggtcaa
     6421 agcctttctg ctgctcaaag catcgggttt gaccctcacg aaaagcacgc tgtggcagct
     6481 tgagtcgctg ctgaacggca cactcccgca agaaatcacg caacacgaca aaacgcgcct
     6541 ttttgccgaa ttgaaaaacc atccgcccgt cgccgccttt ccgccgcagg aagaaatgga
     6601 acacttcatg cgcgaagcac tgcgccaagc agaacaatcc gccgctgacg gcgaaatacc
     6661 cgtcggggca gtcatcgttt cagacggcaa aatcatcgca tcggcacaca acacctgcat
     6721 tgccgactgc aatgtcagct gccacgccga aatcaacgcc ttggcacagg caggcagcga
     6781 aatgcaaaac taccgccttg acggatgcga catctacatc accctcgaac catgcgccat
     6841 gtgcgcgtcc gcactgatac aggcacgaat caggcgcgtg atctacggtg cggcagaacc
     6901 caaaaccggc gcggcaggaa gcatcgtcaa cctgtttgcc gacaaacgcc tcaatacgca
     6961 caccgccata cggggcggaa tcctgcaaga agaatgccgc gccgtattaa accgtttttt
     7021 ccaaaacaaa agaaaaggtt gaacctatga ccgccttgct cgtcatcctc gccctcgccc
     7081 tgatagccgc cggtacggcg ggcatcgttt accccgccct gcccggattg gcattgatgt
     7141 ttgccggaac ctggctgctc gcctactccg gcggctacca aatctacggc gcgggcgttt
     7201 tgtggacggt cggactcatc agccttgccg gcatactggc ggactatgtg gcaggcatat
     7261 gggggacaaa atataccgga gcgggcaagc tcgccgttcg cggcgcattg gtcggcagca
     7321 tcatcggcat atttttctcc cttcccggac taatactcgg tccctttatc ggcgcggcgg
     7381 caggcgaact gatcgatcgg cgcaatatgc ttcaggcagg taaagcgggc ttgggtacgc
     7441 tgttggggct tatcgtcggt acgacattca aaatcggctg cgccgtatcc atcttgttta
     7501 tcctgttggt gaaatacatc gcctacctgt tttaacccct tcagacggca taccggcaaa
     7561 ttgctataat gccgtctgaa cccttttcac aaagcaccat atggcaaacc aaagacctat
     7621 ctacggcttc catgccgtca acgcccgatt gtggcaaaac cccaaatcca tcgtcgaact
     7681 ctatgtccaa gaaggcaaaa acgatgtacg cacgcgcgaa gtgttggaaa aggcggcaaa
     7741 cgaaaacatc cgcgtatatt ttgccgatgc cgaccgcctc aacgccatca gcaaaggtgc
     7801 gcgccatcag ggcgtggtcg gatttatcga tgcctccaaa aaccacgtcc acctcgaaga
     7861 cgtattggaa aacttaagcg aaccgccgct gctgctgata ctcgacggca tcaccgatcc
     7921 gcacaacctc ggcgcgtgcc tgcgtaccgc cgacgcaatg ggcgtacatg ccgtcatcgc
     7981 accgaaagac aaaagcgcgg ggctgaacgc caccgtcagc aaagtcgcca gcggcgcggc
     8041 ggaaaccgtc ccctacatca ccgtaaccaa cctcgcccgc accctgcgcg agctgaaaga
     8101 atacggcatt tggatcatcg gcaccgatat gggcggcaac gccgaccttt accattgcga
     8161 tctgcccgac agcgcggcat gggtgatggg caacgaaggc gacggtatgc gccgcctgac
     8221 acgcgaacat tgcgacatgc tggtgtccat acccatgttc ggcacggtcg aaagcatgaa
     8281 cgtctccgtg tctgccggaa tggtattaag cgaaacccgc cggcagcgcg tattaaaaaa
     8341 cgaaaaagcg taagcctcaa aaacaatgcc gtctgaaaca gggattctgt ttcagacggc
     8401 attcgttttc aaaatcagtg ttccaagtgg tcggtatcaa acttgaccgc cgcctcggtt
     8461 ttatcttcgg gcaagaccaa attcagcaag actgccgtaa tgccgccggc ggaaatagag
     8521 ttttggaaca agacaggcag gtttttaaac acttccggct caaacgccac gcccaagccc
     8581 aagccgaccg aagtcgccgc aatcaccgcc tcgcgcctgc ggatgccgtg gctgaccaaa
     8641 atccgcacac ccgcaatcgc aatcaagccg aacatcaaaa ccatcgcgcc gcctaaaacc
     8701 ggactcggaa tcgtcgtaaa cgcgcgtccg acaacgggga acagacccaa cagcaccaaa
     8761 atcacggcaa tatatttgcc cacatggcgc gaagccacgc cggtcatctg aatcacgccg
     8821 ttgttttgcg caaacgttgt cagcggcagc gaacccaaag ccgtcgcaat caccgacacc
     8881 aagccgtctg ccaacacgcc gccgcgcagg cgtttggtgt attcctcgcc ttcaatcggc
     8941 tgatcggaca ccattgccgt tgcggtcaaa tcgccgaccg cctcaaacac gctcaacagg
     9001 aaaatcgcac ccgccacaat aaatgcgtgc cagtcaaaag caaaaccata tttaaacggt
     9061 acgggcagcg taaccagcgg caggttttgc agtgccgaaa aatccacctt gcccaaaaac
     9121 agcgcgacga tatagccggc aatcagaccg accgcaatgc cgctcatccg cagcagcggg
     9181 tttttcatgc agttgaacac cagcacaatc agcagcaccg gcgatgccag ccccaagttt
     9241 tccatcgagc cgaacgtgcc gtccgccttt gcgccgaagc cgccgccgaa atcggtaata
     9301 ccgacgtgta ccaaactcaa gccgatcagc atcaccacca caccgctgac cgtcggcgta
     9361 atcacttttt tcaaatacgg cagaagccac gccgagaaac acaccaaaaa cgcgccgaca
     9421 aacgatacgc ccaagagcgt cgaaatcatc gcatccttag tcaaaccgcc ctctttcatc
     9481 cccgcgccca gcgcaatcat gacggtaacg aacgagaaat tcaccgactg gatagacagc
     9541 atccccgaac cgaccggccc gaagcggttg acctgcaaat aagtgccgac acccgacgca
     9601 accatcgcca tcgacacgag atacgccgtc atctccaccg gcagttccag cgcgccgccc
     9661 acaatcagcg cgggcgtaat catcggcacg aaaatcgcca aaagatgggt aaccgcgctc
     9721 aacagcgcat taccgaacgg cggcctatcc tccaaaccgt acaccaaatc aggcgcatcc
     9781 gccccttttc ccatatgacc gctcataatg tttccttgct tcaaatgttg aaaaaaccaa
     9841 agcgcggatt gtagcgaatc cgccataccg cttcaatttc gatttcggac ggcatacgcg
     9901 cccgtccggc aaacagattc cgaaccgtcg ccgccggaat atcgtttgca aaacgcaata
     9961 acctgatttc aaacacaaaa atacaaaccc gacgcatccg ccgccaagcc gccccgtgca
    10021 taccggaccc gccggatttc acacctgcgg taaagtaggc tacaatcgga agcattaccg
    10081 ccccctacac cacaactgat aaaaggttct gtatgttaca aggtagcctg gttgccctga
    10141 ttaccccgat gaatcaagac ggcagcatcc attacgaaca actccgcgac ttaatcgact
    10201 ggcacattga aaacggcacg gacggcatcg tcgccgtcgg cacgacaggc gaatccgcca
    10261 ccctctccgt cgaagaacac accgccgtca tcgaagccgt cgtcaaacac gttgccaaac
    10321 gcgtccccgt catcgccggc acaggggcaa acaacaccgt cgaagccatc gccctttccc
    10381 aagccgccga aaaagccggg gcggactaca ccctctccgt cgtcccctat tacaacaaac
    10441 cttcgcaaga aggcatgtac cgccatttca aagccgttgc cgaagccgcc gcgattccga
    10501 tgatactcta caacgtaccc ggcagaaccg tcgtcagcat gaacaacgaa accatcctgc
    10561 gccttgccga aatccccaac atcgtcggcg tgaaagaagc cagcggcaac atcggcagca
    10621 acatcgaact catcaaccgc gcgcccgaag gcttcgtcgt cctttccggc gacgaccata
    10681 ccgccctgcc gttcatgctg tgcggcggac acggcgtgat taccgtagcc gccaacgccg
    10741 cgcccaagct ctttgccgat atgtgccgcg ccgcgctgca agtcgacatt gccctcgcac
    10801 gcgaactcaa caaccggctg attccgattt acgacaccat gttctgcgaa cccagccccg
    10861 ccgcaccgaa atgggcggta tccgccttgg gcagatgcga accgcacgtc cgtttgccgc
    10921 tcgttccgct gacggaaaac ggacaagcaa aagtccgcgc cgccctgaaa gcctcaggac
    10981 aactttaaat atagccatgc cgtccgaacc gttcggacgg cataacgcaa caaacacttt
    11041 aatatccatc acacaggatg acacgatgac ccatatcaaa cccgtcattg ccgcgctcgc
    11101 actcatcggg cttgccgcct gctccggcag caaaaccgaa cagcccaagc tcgactacca
    11161 aagccggtcg caccgcctga tcaaacttga agtcccacct gatttgaaca accccgacca
    11221 aggcaacctc taccgcctgc ctgccggttc gggcgccgtc cgcgccagcg atttggaaaa
    11281 acgccgcaca cccgccgtcc aacagcctgc cgatgccgaa gtattgaaaa gcgtcaaagg
    11341 tgtccgcctc gagcgcgacg gcagccaacg ctggctcgtt gtcgacggca agtctcctgc
    11401 cgaaatctgg ccgctcctga aagccttttg gcaggaaaac ggcttcgaca tcaaatccga
    11461 agaacccgcc atcggacaaa tggaaaccga gtgggcggaa aaccgcgcca aaatccccca
    11521 agacagcttg cgccgcctct tcgacaaagt cggcttgggc ggcatctact ccaccggcga
    11581 gcgcgacaaa ttcatcgtcc gtatcgaaca gggcaaaaac ggcgtttccg acatcttctt
    11641 cgcccacaaa gccatgaaag aagtgtacgg cggcaaagac aaagacacga ccgtatggca
    11701 gccctccccg tccgatccca acctcgaagc cgctttcctg acgcgcttta tgcaatattt
    11761 gggcgttgac ggacagcagg cggaaaacgc atcggcaaaa aaacctaccc ttcccgccgc
    11821 caacgaaatg gcgcgtatcg aaggcaaaag cctgattgtc tttggcgact acggcagaaa
    11881 ctggcggcgc accgtgctcg ccctcgaccg catcgggctg accgtcgtcg gtcaaaacac
    11941 cgaacgccac gccttcctgg ttcaaaaagc cccgaacgaa agcaatgcag ttaccgaaca
    12001 aaaacccggc ctgttcaaac gcctgctggg caaaggcaaa gcggagaaac ctgccgaaca
    12061 gccggaactg attgtctatg cagaacctgt cgccaacggc tcgcgcatcg tcctgctcaa
    12121 caaagacggc agcgcatatg ccggcaaaga cgcatccgca ttattgggca aactccattc
    12181 cgaactgcgt taaaccattc aaaacaaaag cagcttccaa acagaagctg cttttttatg
    12241 cggaactttt tacaacaaat ggggcaaaat atcctcaact gcctgaacca acgcatccac
    12301 caaaggcgga tcgaacgcac gatgccctgc ctgaaccacc ctcaattccg cttcgggaaa
    12361 ggctttcgac agcgcccacg cactctgcat cggcgtacac aaatcatacc gcccctgcac
    12421 gataatggtc gggatatgcc gtattttgcc gatattgttc aaaatcgccc tatcgccctg
    12481 caaccagccg ccgttgacaa aataatggtt ttccaaacgc gcaatcgcca gtgaggcata
    12541 agcatcttca tccacatcct ccggctcgaa acggatcaga tagctttccc aatccgccca
    12601 agccttcgcg gcagacaggc acacttcttc atcttgatga aacagcaacc cgtgatacgc
    12661 ctcaatcagc cggttccgcc gattttcagc aatcggcgcg acaaattttt gccattgttc
    12721 cggataaatc cggctcacac cgcccgcctc gttcagccac gccgtttcag acggcctgca
    12781 caaaaatatc ccgcgcaata ccaatccctt tacccgttca ggatgggttt gggcataagc
    12841 cagcgacaaa gtgctgcccc acgaaccgcc gaacaccagc catttcctga tacccagcat
    12901 ttcacggact ttttcaatat ccgccaccaa atcccaagtc gtattgtctt ccgcacaagc
    12961 atacgggcgc gaacgtccgc aaccgcgctg gtcgatgatg acgatacgga acacatcggg
    13021 attgaaaaaa ccccgacatt caggcgaagc ccccgcgccc ggcccgccat gtaaaaaaat
    13081 gaccggcaca ccgtcgggat tgcccgactc ttcccaataa atttgatgaa tttcagacac
    13141 ctgcaagtat ccgctatgaa aaggctgttt tatctcatac atcatcttct cccatattgc
    13201 ttctgacaat ccgaacattc ccacacgcaa catcacgcac gagacacaag ccgccccgaa
    13261 aggcaaatgc cgaaaaatcg cgatatagtg aattaacaaa aatcaggaca aggcggcgag
    13321 ccgcagacag tacggatagt acggaaccga ttc
//
LOCUS       DAWHRA010000049        13301 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWHRA010000049 DAWHRA010000000
VERSION     DAWHRA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.049"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(99..1460)
                     /gene="accC"
                     /locus_tag="WIB30_001034"
     CDS             complement(99..1460)
                     /gene="accC"
                     /locus_tag="WIB30_001034"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ3797116.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(1574..2026)
                     /locus_tag="WIB30_001035"
     CDS             complement(1574..2026)
                     /locus_tag="WIB30_001035"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ3797117.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQQ
                     VKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(2008..2265)
                     /locus_tag="WIB30_001036"
     CDS             complement(2008..2265)
                     /locus_tag="WIB30_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ3797118.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            2382..3422
                     /gene="queA"
                     /locus_tag="WIB30_001037"
     CDS             2382..3422
                     /gene="queA"
                     /locus_tag="WIB30_001037"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ3797119.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
     gene            complement(3896..4474)
                     /locus_tag="WIB30_001038"
     CDS             complement(3896..4474)
                     /locus_tag="WIB30_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ3797120.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMT
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            4713..5612
                     /gene="crgA"
                     /locus_tag="WIB30_001039"
     CDS             4713..5612
                     /gene="crgA"
                     /locus_tag="WIB30_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ3797121.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(5841..9056)
                     /gene="carB"
                     /locus_tag="WIB30_001040"
     CDS             complement(5841..9056)
                     /gene="carB"
                     /locus_tag="WIB30_001040"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ3797122.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAMNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECEARPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(9096..9707)
                     /locus_tag="WIB30_001041"
     CDS             complement(9096..9707)
                     /locus_tag="WIB30_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797123.1"
                     /translation="MINIIATWAMALTYIFLLLFIIVFIPIQLYLENIKKKKFKSRII
                     DILKNNHNNLDLNDIKQMTEAVNLNNFAARKIIKQLYYTDELNLNLVRQLQKEIQQEE
                     PFDGCSDELKPTLIGINELLETHGSESQKHLLTPIISELKELNQIKHDHKKMKTQSYI
                     AYIIAIISFFIGSISFYYTITAPSGKEIANTVVEQLKANQNQQ"
     gene            complement(10555..10944)
                     /locus_tag="WIB30_001042"
     CDS             complement(10555..10944)
                     /locus_tag="WIB30_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ3797124.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(11517..11642)
                     /locus_tag="WIB30_001043"
                     /pseudo
     CDS             complement(11517..11642)
                     /locus_tag="WIB30_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(11688..12821)
                     /gene="carA"
                     /locus_tag="WIB30_001044"
     CDS             complement(11688..12821)
                     /gene="carA"
                     /locus_tag="WIB30_001044"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ3797125.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIKAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2898 a         3338 c         3651 g         3414 t
ORIGIN      
        1 ttaatccact atatcgaaat cggtttcaga cggcgcgggg caagggaaat gccgtctgaa
       61 cgcggacggg cggcttcaga cggcatatcg gcggcggttt acttgtcctg tttggctttg
      121 cgatcttcca gccagtgttc caaatagtgg atgctgacgc cgcctttttg gaaacccgca
      181 tcggcgaaca ggtcgcggtg aagcggcgta ttggttttga tgccggttac cgccagctcg
      241 gcgagtgcga cgcgcatttt cgccattgcc tgttcacgcg ttttgccgtg tacgcagatt
      301 ttgccgatca ggctgtcgta gtacggtggg atgcggtagc cttggtaaat gtggctgtcc
      361 acgcggatac cgaagccgcc gggcaggtgg cagctttcaa tcgggcccgg gctggggatg
      421 aagttgtacg ggtcttcggc attgatgcgg cactcaaacg cgtggccttc gacttgaata
      481 tccttttgtt tgtattgcaa aggcaggccg gcggcgatgc ggagttgctc ctgcacgatg
      541 tccacgccgg taatcagctc ggtaaccgga tgctcaacct gaacgcgcgt attcatctcg
      601 ataaagaaaa attcgccatc ttcgtacagg aattcaaacg tacctgcgcc acgatagccg
      661 atgcgtttgc aggcgtcggc acaggcgttg ccgattttgg cgcgttcttt ttcagtgatg
      721 aacggagccg gtgcttcctc gatgactttt tggtggcggc gttgcagcga acagtcgcgc
      781 tcggcaaggt agatggcgtt gccgtgttcg tcggcaatca cttggatttc gacgtggcgc
      841 ggacgttgca gatagcgttc catgtaaacc atcgggttgc cgaatgccgc gcctgcttct
      901 gccttggtca tttcgacaga ttggaggagg tcttcttttt tctcgaccac gcgcataccg
      961 cgaccgccgc cgccgccaga ggctttgata atgacgggat aaccgacttt atcggcgatt
     1021 ttgaggattt cttcgccgtc gtcgggcaat gcgccgtcag aaccggggac gcagggtacg
     1081 cctgccgcta tcatcgcgtg tttggcggag actttgtcgc ccatcaggcg gatggtgtcg
     1141 ggtttcgggc cgataaaggt aaagccggac tgctcgacct gttcggcgaa atcggcgttt
     1201 tcggcaagga aaccgtagcc cggatggaca gcgtccgcgc agcttacttc ggcggcggcg
     1261 ataatggcgg ggacgttaag gtagctttgc gcggaagcgg cagggccgat gcacacggat
     1321 tcgtcggcga gtttgacgtg caggctgtct ttgtcggcct cggaatgcac ggcgacggtg
     1381 gcaatgccca tttcgcggca ggcacggagt acgcgtaatg cgatttcgcc tcggttggcg
     1441 attaaaactt ttttcagcat gatgactgtt cctgtggttc cggcaacccg tccgtaaaaa
     1501 aaaaggtgcg ggaacgccga agggggaaag catttcagac ggcatcggaa gtttatgccg
     1561 tctgaaaaca ggattatccg atgatgaaga gcggttcgcc gaattcgacg ggcgtaccgt
     1621 tttcgaccag aatttctttg accgtgccgg atttttcggc ttcgatttcg ttcatcagct
     1681 tcatcgcttc gatgatgcac agcgtgtcgc cggctttaac ctgttggccg acttcgacaa
     1741 aaggcgcggc attcgggccg ggtgcgcggt agaacgtgcc gaccataggc gatttttgcg
     1801 cgtcggacaa atcgcgggcg gcaggtgcgg cgggcgcgga tgccgcaacg ggtgcggcgg
     1861 caggcgttac ggccggcgcg gcggcaggta cgggcgcggc gtaaacgggt gcggcggcga
     1921 tggttcgggt gatgcggact ttttcctcgc cttcggttac ttcgatttcg gcgatacccg
     1981 attcttcaac caaatcaatc agttttttta atttgcgcaa atccatttct gttcctttaa
     2041 aggctgccgg tgttccggcg ggttgttgcg ttggtttttc agaaaacagc cggcttgcgg
     2101 caacggcgca aaccgggcgg aatggtgttc ggatacggtt ttcctgtggt ggaaaacaac
     2161 cgtgagaatg tgtttatttt cccgaagttg tcggtaaatg tccagtaaaa tatcaaaaaa
     2221 cggcggtttt tgggggaaat gtgcaggaag cttgattttg cgcacaagat gccccgggtc
     2281 aagagtgttt attctaatct gttggttttt cggacaaaga tgccgtctga aaaagggcta
     2341 aagtgcgtat aatggcggct tgcccgaacg agagtgtaaa aatggatatt tcagattttg
     2401 attttaccct gcccgaaaag ctgattgccc agcatccgcc cgaagtgcgc ggcagcagcc
     2461 gccttttggt tgcgttgccc gatatgccgc tgcaagaccg ggtgttcggc gatttgccgg
     2521 attatgtcga ggcgggcgac gttttggtgt tcaacaacac caaagtcatg aaggcgcggc
     2581 tgtttgggca gaaagacagc ggcggcagga tcgaagccct gattgagcgc gtgttggaca
     2641 accataccgc actggcgcac atccgttcgt ccaagtcccc caagcccggt atggggctgg
     2701 tgtttgaagg cggcatccgt gccgtgatgg tcgggcgtga gggcgaactg ttctgcctgc
     2761 gttttgaagg cggtcaaacc gtttacgaac ttttggaaca gaacggccat ttgcctctgc
     2821 cgccttatat cgaacgtgcc gccgacgcgg acgacgacag ccgttatcaa accgtttatg
     2881 ccaaatatca gggcgcggtc gccgcgccga cggcgggcct gcattttacg gaagaacttt
     2941 tgcaccgtct gaaagacaaa ggcgcggtaa ccgcagaagt aactctgcac gtcggtgcgg
     3001 ggacattcca acccgtgcgt gtggaaaaaa tcgaagaaca caaaatgcac agcgaatggt
     3061 ttgaagtgcc gtctgaaacc gccgccgccg ttgaggcggc aaaagcccgg gggaacaaag
     3121 tctgggcggt cggcacgact tccatgcgcg ccctcgaatc tgccgcgcgt gcgacgggtc
     3181 gtctgaaagc ggggcagggc gacaccgata ttttcatcac gccgggctac cgttttaatg
     3241 ttgccgacag gctggttacc aatttccatc tgccgaaatc gacgctgctg atgcttgtca
     3301 gcgcgttttc gggtatgggt catatccgcg ccgcgtatcg ttatgcggtc gaacgcgaat
     3361 accgtttctt cagctacggc gatgcgatgg ttttggggcg gaacgaaggg gtggtacgtt
     3421 agaacagcct caagtctatt gggggaagac aagtgccgtc tgaacggatg ttcggacggc
     3481 attcttttgt tgatttgaat gaaaggaact gcatgacata tagtggatta aatttaaatc
     3541 aggacaaggc aacgaagccg tagacagtac aaatagtacg gcaaggcgag gcaacgctgt
     3601 actggttttt gttaatccac tatagattca ggcgcgagag gcgagaaact cagtcattgt
     3661 tgatgacgcg caatacgtta aaattcattc cgttttggtc gtataaacat attacatacg
     3721 ccattttttt gtattcaagg ctgcctgaaa acagtgggca agaatatcta acccaacgaa
     3781 aagctgaaaa agcaacaaaa aataatctgc accttagtcg gttggttagt ccaaccttag
     3841 gggtgcagac cattcaatct ttcaggctgc ctttgtttgt ttataagata aatttttagc
     3901 cgaacacttt ttccaagtgt gcctgataat ctgccaagta tttttccact tgtggatttt
     3961 taaccacatc gttacataag aatgtcggca ggcgggtcat acccaaaaac tcgttggctt
     4021 tgtggaagtg catatacaaa acatcaacgc ctttgccttc aaagaaatcg ccttcgcggg
     4081 taaacgcctc aatcggcgca ttccaagtca gtgaaagcat atgttttttg ccttgcaaca
     4141 agccgcctgt gccgtagccc tcagtcggat tgacgcggtg tctgccgtcg ctttggtaga
     4201 gtttgccgtg tccagcggtt aatacttcgt ctatgtattt tttcactgtc caaggctcgt
     4261 gcatccacca gcccggcatc tgccaaatca cagcatccat ccaaacgaat ttttcgattt
     4321 ctgcctcaac atcatagccg gcatcaatca cggtttcttg aacattgtgt ccgagcgcgg
     4381 tcaaaacttc tttcgctttt ttgtgaagcg tgcggtttaa cccgccgtga gaatgtccga
     4441 acgctttgcc gccgtctaat aataaaatat tcatttgtta cctcgtttgt gaattgatga
     4501 atgtattgtg ccgttttact ttataattta aaagtgcaaa aataagaaaa cacttttgcg
     4561 tcaaatgaaa taatcagatg ttccaacaca cgggatggca cataaagcac cgccctgtgt
     4621 gccgtcctga ttcggaaggg gttacacccc tcccaaataa agtctgatcc tgccgcccta
     4681 aagggcgggg ttccaaccga aaaggaaaca cgatgaaaac caattcagaa gaactgaccg
     4741 tatttgttca agtggtggaa agcggcagct tcagccgtgc ggcggagcag ttggcgatgg
     4801 caaattctgc cgtaagccgc atcgtcaaac ggctggagga aaagttgggt gtgaacctgc
     4861 tcaaccgcac cacgcggcaa ctcagtctga cggaagaagg cgcgcaatat ttccgccgcg
     4921 cgcagagaat cctgcaagaa atggcagcgg cggaaaccga aatgctggca gtgcacgaaa
     4981 taccgcaagg cgtgttgcgc gtggattccg cgatgccgat ggtgctgcat ctgctggcgc
     5041 cgctggcaac aaaattcaac gaacgctatc cgcatatccg actttcgctc gtttcttccg
     5101 aaggctatat caatctgatt gaacgcaaag tcgatattgc cttacgggcc ggagaattgg
     5161 acgattccgg gctgcgtgca cgccatctgt ttgacagccg cttccgcgta atcgccagtc
     5221 ctgaatacct ggcaaaacac ggcacgccgc aatctacaga agagcttgcc ggccaccaat
     5281 gtttaggctt caccgaaccc ggttctctaa atacatgggc ggttttagat gcgcagggaa
     5341 atccctataa gatttcaccg cactttaccg ccagcagcgg tgaaatctta cgctcgttgt
     5401 gcctttcagg ttgcggtatt gcttgcttat cagatttttt ggttgacaac gacatcgctg
     5461 aaggaaagtt aattcccctg ctcgccgaac aaacctccga taaaacacac ccctttaatg
     5521 ctgtttatta cagcgataaa gccgtcaatc tccgcttacg cgtatttttg gattttttag
     5581 tggaggaact gggaaacaat ctctgtggat aattctataa aaacctgcca taaaattgtt
     5641 tttactcact taataacttt gagaaaaaat aaataagatg atgtatagcc gcggcaatgg
     5701 cagtctatat ggggagcgtg atagggctgt tccttatgta cggcttgtgt gtggcaacat
     5761 cggctttcca aatgacaaaa tgccgtctga aaccgtaaaa aaaaaaggtt tcagacggca
     5821 ttttcaacct gattcaggca tcagttgcgg tttttcaaac gcccgtgcag ttcttgaacg
     5881 ctgtacacgc ccaagtggtc gcggcttttc gcgccttcgc tcatcgcttc gccgccggcg
     5941 gtggtggtgt attgcggcac gcgttgctgc aatgcgcttt ggcggatgat gtggctgtcg
     6001 gacacggatt gcggatcgct ggaaacggta ttcacgacca gtgcgatttc accgtttttc
     6061 agcgcgtcgc cgatgtgcgg gcggccttcg gggactttat tgatggtttg gacaatcagc
     6121 ccgtgttcgg tcaggtattg cgccgtgccg cgcgtggcgc agatgccgta gcctaaggct
     6181 tggaagtttt tggcggtttt aatgacgcgt tctttgtctt cttcgcgcac ggagaggaag
     6241 attttgccgg tcgggttgag gcgttcgcct gcgccgagtt gggctttgta gtaggcttcg
     6301 ccgaagcttg cgcccacgcc catgacttcg ccggtggagc gcatttccgg acccaaaatc
     6361 gtatccacgc ccgggaattt gatgaagggg aacacggctt ctttaacggc atagaaatca
     6421 gggacgactt ctttttccac gccttgttct ttcagggaaa tgcctgccat acagcgtgcg
     6481 ccgactttgg cgagcggcac gccggtggct ttggagacga aggggacggt gcggctggcg
     6541 cgcgggttca cttccaatac gaacactacg ccgtcctgca cggcaaactg cacgttcatc
     6601 agtccgacca cgcccagcgc gtacgccatc gctttggttt ggcggcggat ttcgtcttgg
     6661 atttcttcgc ttaaggagta gggcggcagc gagcagccgg agtcgccgga gtggatgccc
     6721 gcctgttcga cgtgctgcat gatgccgccg ataaccacat ctttgccgtc tgaaacgcag
     6781 tccacatcga cttcaatcgc gttgttcagg aagaagtcga gcaacacggg gctgtcttcg
     6841 gaaacctgca cggcttcgcg catatatttt tgcaactgct cggcagagtg gacaatctgc
     6901 atcgcgcgtc cgcccaaaac ataagacggg cgcacgacca gcggataacc gatttcttcg
     6961 gctttgacga gtgcttcttc ttcgttgtgg gcgatgcggt tgggcggctg gcgcaggcct
     7021 aagtcgttca acactttttg gaagcgttcg cggtcttcgg cggcatcgat gctgtcggcg
     7081 gacgtgccga tgatgttcac gccgttttca accagcgcgt tggcgagttt gagcggggtt
     7141 tgaccgccgt aatgcacaat cacgccccac gggttttcgg tgcggacgat ttccaacacg
     7201 tcttccaatg tcagcggctc gaaatagagg cggtcgctgg tgtcgaagtc ggtggacacg
     7261 gtttctgggt tgcagttgac cataatcgtt tcaaaaccgg attcgcgcag ggcgagcgcg
     7321 gcgtgaacgc agcagtagtc aaactcgatg ccctgaccga tgcggttcgg gccgccgccg
     7381 agaatcatca cttttttgcg gtcggaagga cgcgcttcgc attcctcttc gtaagtggaa
     7441 tagaggtagg cggtttcggt ggcgaactcg gcggcacagg tgtcgacgcg tttgtaaacc
     7501 ggatgcagct tcagcgcgta gcggtgttcg cgaacttctt tttcgcttac gttcaacaat
     7561 tgtgccaaac gtttgtcgga gaagcctttg cgtttcagac ggcgtagggc ggcgaaatcc
     7621 aaatcactca aaatgccgtc tgaaaccgct ttttcttcct tcatcaagtc ttcgatttgc
     7681 gccaggaacc aagggtcgat ggcgcagatt tcgtggattt cttccagcgt gaagccggcg
     7741 cggaacgcgt ctgccacaaa cagcatacgc tcagggccgg ggttcgccag ttcgcggcgg
     7801 atttccgctt tgtcggagct tctcggattg aagccgcaca agccggtttc caagccgcgc
     7861 aaggcttttt ggaagctttc ctgaatcgtg cggcccatcg ccatcacttc acccaccgat
     7921 ttcatctgtg tggtcaggcg gtcgtctgcg gcagggaatt tttcaaacgc gaaacgcggg
     7981 attttggtta cgacatagtc gatggacggc tcgaacgacg cgggggtttt gccgccggtg
     8041 atgtcgttgc gcaactcgtc cagcgtaaag ccgaccgcca gcttggccgc caccttcgca
     8101 atcgggaagc ccgtcgcttt ggaagccagc gcggacgaac ggctcacgcg cgggttcatc
     8161 tcaatcacaa tcatctcgcc gttttcaggg ttcatcgcaa actgcacgtt tgagccgccc
     8221 gtgtccacgc cgatttcgcg caataccgcc aacgaagcgt tgcgcatgat ttggtattcc
     8281 ttgtccgtca gcgtttgcgc cggcgcgacc gtaatcgagt cgcccgtatg cacgcccatc
     8341 gggtcgaagt tttcaatcga gcagatgatg atgcagttgt cgttcttgtc gcgcactact
     8401 tccatctcgt actctttcca gccgagaacg gactgctcaa tcagcagctc gtgcgtgggc
     8461 gaggcatcga aaccgcgttc gcaaatcgcc aaaaactcgt ctttattgta ggcaatgccg
     8521 ccgcccgaac cgcccatcgt gaaagaagga cgaatcaggg tagggaagcc gacctgttcc
     8581 tgcgccgcca aagcttcgtt cattgtgtgg cagacaaaag atttcgggca agacaaaccg
     8641 attttttcca tcgcctcttt aaagcggccg cggtcttctg ctttgtcgat ggcgtcttcc
     8701 gtcgcgccga ttaattcaac attgtatttc gccagcacgc cgttgcgcgc caaatccagc
     8761 gcacagttca gcgcagtttg accgcccatg gtaggcagaa tcgcatcggg ccgctctttg
     8821 gcaataattt tttccaccgt ctgccacata atcggctcga tgtaggtaac atccgccatt
     8881 tcggggtcgg tcataatcgt ggcggggttg gaattcacca aaatgacttt atagccttct
     8941 tcacgcaagg ctttgcaggc ctgtgcgccc gaatagtcaa attcgcaggc ctgaccgata
     9001 acgatagggc cggcgccgat gataaggatg gattttaggt cggtacgttt gggcatgatg
     9061 ggtctttcta tgaaatcaaa tgtttagcta aaaatttatt gctgattttg attggctttt
     9121 aattgctcta cgacagtatt ggcaatctct tttcctgatg gggctgtaat tgtgtaataa
     9181 aagctaatac tgcctatgaa gaaactgata atggctatga tataggctat ataactttgt
     9241 gttttcattt ttttgtgatc atgtttgatc tgatttaatt cttttaattc agaaataatt
     9301 ggagttaata aatgtttttg actttcagat ccatgcgttt ccaaaagttc atttattcct
     9361 attagtgttg gttttagttc gtctgagcag ccatcaaaag gttcttcttg ttggatttcc
     9421 ttttgtaact gtctaactaa atttaaattt aattcatcag tgtaatatag ttgcttaata
     9481 atttttctgg cagcaaaatt attaaggttt actgcttctg tcatttgttt tatatcatta
     9541 agatctaggt tattgtgatt attttttaat atatcaatta ttctagattt aaatttcttt
     9601 ttctttatat tctctaaata tagctgaatt ggaataaaaa caattataaa gagcaaaaga
     9661 aatatatatg ttagtgccat agcccatgtg gcaattatat ttatcattta tcaatatctt
     9721 ccatttggtt tatatattgt ctcataataa gcaagccgta gctcgcatgc agggtgtgtg
     9781 cggtacgcac gcgttctcca ttttccctgc aaccccaacc caccgtgtgc gtgccttgcg
     9841 gcacacaccc taccgatggg ttcaaaggtc gtctgaaaaa acgctcgcgg cgtttgttcc
     9901 atctgccttg cgataaaggc tgcctgaaaa attcccgctg ttgatatttc gggcattctt
     9961 tctcaaaccg cctgcaaccg caaaatccgc tgcaacctgc cccctccccc gtgggggagg
    10021 gttggggaga gggcattccc cgaatttcgg caacctttcc caacaactca accaccgaaa
    10081 tacaagcctt gtggcttgtt gccctctctc tagctctctc ccacgggaga gaggacggag
    10141 tggctgtagg gctaagggtt ctgcaaggaa acaggttgtg caggttgctt tttgcttttc
    10201 agacgacctg aaatcaacgg taaatccaag tcgcttttca aaccgcctgc aaccgcaaaa
    10261 tctgctgaaa cctactccct cccccgtggg ggagggctgg ggagagggca ttctccgaat
    10321 ttcggcaacc tttcctaaca ctctaaccgc caagacacaa gccttgcggc ttgttgccct
    10381 ctctctagct ctctcccacg ggagagagga tggggtggct gttggggcta aggtttctgt
    10441 aaactaatca ggttatgcag gctgcttttt gtttttcaga cggcctttat aaatgctcaa
    10501 ttcagtcggc tttatcctat tattttcaat aatccaatca aaatcagcca tctgctatcg
    10561 tgcagcttgc ttttccaatt cttgcaacac gagcaggatt tctgtcagca catcgtttgt
    10621 ctgctgtaaa atctcgtgat tccaaaaccg cagtacggta aagcccaagc tgttgagata
    10681 tgccgtccgc gcgtggtcgt atgcggcttg ttctgtgtgc tgccctccgt ctgcttcgac
    10741 aatcaacttg ggcgttacgc acataaaatc aacaatatag tttcccatcg gctgctggcg
    10801 gcggaattta tagccgttca gacggcctgc ccgcaggtgc tgccacaatt ttgcttccgc
    10861 ctcgctcatt tcttggcgca tggctttggc gcgttggcgc aagatggggt tgtcggtggt
    10921 caagagtttc tcgtgcgggt tcattttgtt gtcgtgctgt ttgctgccct ctctctaact
    10981 ctctcccacg gggagagagg acggggcggt tgttggggtt aaaggggctg taaggaaaac
    11041 aagtcgttca ggccactttt tacttttcag acgacctgca atcaacggtt gtaaatccaa
    11101 gttgcttttc aaaccgcctg taaccgcaaa acttgctgca acctaccccc tcccccgtgg
    11161 gggagggctg gggagagggc attctccgaa tcgcggcaat ctttccctaa ttccctaact
    11221 gcctcaatac aagcctttcg gcttgttgcc ctctctctaa cgctttccca cgggagagag
    11281 gacggtgcgg ctgtagtgat taagggtttt gcaaggaaaa caaggaaaac aagttgtaca
    11341 ggctgttttt tatttttcaa gcgacctgaa atcaacgctt gtaaatccaa gtcgcttttc
    11401 aaactgcctg caaccgcaaa atctgctgca acctaccccc tcccccgtgg gggagggctg
    11461 gggagagggc attttccaag ttgcggcaat ctttcccaaa tcccttagtc ttctaaaata
    11521 caagccttac ggttgttgcc aaatgaaact tcttccatac ccaaaacttg cgtgtaaaac
    11581 gcaatggttc ggtcaatgtc ggcaacggtc agtaccagat ggtcgagtgc gctaatcttc
    11641 atttcccgct ttctttttca ggcggcggca gtgccgtctg aaagccatta tgcccgtttt
    11701 gccgctttca tattgccaat gaatttgtca aacaaatagc cgacatcttg cggaccgggg
    11761 ctggcttcgg ggtggccttg gaagcagaac acaggtttgt cggtcagctc gatgccttgc
    11821 aaagtgttgt caaacaagga tttgtgggta atgcgtgcgt tagcgggcag ggtgtcggca
    11881 tcaacggcaa aaccgtggtt ttggctggtg atgacgactt tgccgctgtc caaatcttgc
    11941 acagggtggt tcgcaccgtg gtggctgaag cgcattttca gggttttcgc gccgatggcg
    12001 aggctgatga gctggtgtcc caagcaaatg ccgaaaatcg gtttgccgct ttccatcagt
    12061 ttttgtacgg ctttgatggc gtaggtgcaa ggctcggggt cgccggggcc gttggatagg
    12121 aatacgccgt cagggttgag tgccaacacg tcttccgcgc tcgtttgggc ggggacgacg
    12181 gtcaggcggc agccgcgcga ggcgagcata cgcaggatgt tggttttcac gccgaaatcg
    12241 taggcgacga cgtggtaagg ctgttcgtca ggggtaacga aacccttgcc caatgcccat
    12301 tcgccttccg tccattcgta agtttccgtg caggaaactt cttttgccaa gtctttgccg
    12361 accatactgc cgaacgcggc gatgagttct tgcgcttttt cgattgtggc atccgcaccg
    12421 gtcagaatcg cgccgccttg cgcgcctttt tcgcgcaaca gcgtggtcag gcggcgggta
    12481 tcgatgtcgg cgatggcgac ggttttgttg cgtaccagat agtcgtgcag gctttcggag
    12541 gcgcggaagt tgctgtgcaa gagcggcagg tcgcggataa tcaggccggc ggcataaacg
    12601 ctgcggcttt cttcatcttc ggcgttggtg ccggtgttgc cgatgtgtgg gtaggtgagg
    12661 gtaacgattt gtttgcagta ggacgggtcg gtcaggattt cctgatagcc ggtcatcgaa
    12721 gtattgaaca cgacttcgcc ggaagtcgaa ccttcgtaac cgattgatgt gccgtgaaat
    12781 acgctgccgt cagcgaggac gaggagggcg ggggtgctca tgatgggaat cctgttttta
    12841 ataaaattct tgacaatgcc gtctgaaggg acttcggcag ggggcatccg gtcggaaaaa
    12901 tccggtcaaa aaaaaacacg ccgcgcaaga tagacgcgta acgtgctttt cgggcaagtg
    12961 cctataccct gaaagcagca tattttaaga cgaaacatac ccgatttcaa gcgcaaacgc
    13021 ggcaggcggg ctgatatgtt cgcgcgaaaa cggaaatccg tggcgtaaaa ccgcccgctc
    13081 cgcgcccact atgcccgtta cgcatttccc cacgtccgtc cggcgaaact atgggaatac
    13141 ccgaatcgtc aggaatacct gaatcgtcag gaatacccga accgtcattc ccgcgcaggc
    13201 gggaatccgg acttgtcggc acggaaactt atcgggtaca acggtttctt gagattttac
    13261 gtcctagatt cccgcctgcg cgggaatgac gcgtttcatt t
//
LOCUS       DAWHRA010000050        13203 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWHRA010000050 DAWHRA010000000
VERSION     DAWHRA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13203
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.050"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            428..1144
                     /locus_tag="WIB30_001045"
     CDS             428..1144
                     /locus_tag="WIB30_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3797126.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            1247..1672
                     /locus_tag="WIB30_001046"
     CDS             1247..1672
                     /locus_tag="WIB30_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797127.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYDPTD"
     gene            1916..2116
                     /locus_tag="WIB30_001047"
     CDS             1916..2116
                     /locus_tag="WIB30_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797128.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            2184..2543
                     /locus_tag="WIB30_001048"
     CDS             2184..2543
                     /locus_tag="WIB30_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797129.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVPNTSSTLKMVRSN"
     gene            2568..3422
                     /locus_tag="WIB30_001049"
     CDS             2568..3422
                     /locus_tag="WIB30_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HEZ3797130.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTLLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGVIA"
     gene            3492..3707
                     /locus_tag="WIB30_001050"
     CDS             3492..3707
                     /locus_tag="WIB30_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797131.1"
                     /translation="MTITEEYSIDIRVTAEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRQALICLTKDNLKNAA"
     gene            3742..4002
                     /locus_tag="WIB30_001051"
     CDS             3742..4002
                     /locus_tag="WIB30_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797132.1"
                     /translation="MKYLIRTALLAVAAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            3999..4292
                     /locus_tag="WIB30_001052"
     CDS             3999..4292
                     /locus_tag="WIB30_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797133.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            4295..4486
                     /locus_tag="WIB30_001053"
     CDS             4295..4486
                     /locus_tag="WIB30_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797134.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            4623..4832
                     /locus_tag="WIB30_001054"
     CDS             4623..4832
                     /locus_tag="WIB30_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ3797135.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(4890..5807)
                     /locus_tag="WIB30_001055"
     CDS             complement(4890..5807)
                     /locus_tag="WIB30_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEZ3797136.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMGRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(6675..7154)
                     /locus_tag="WIB30_001056"
     CDS             complement(6675..7154)
                     /locus_tag="WIB30_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ3797137.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(7590..7970)
                     /locus_tag="WIB30_001057"
                     /pseudo
     CDS             complement(7590..7970)
                     /locus_tag="WIB30_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(8083..9156)
                     /locus_tag="WIB30_001058"
     CDS             complement(8083..9156)
                     /locus_tag="WIB30_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEZ3797138.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWIYPTGGRSWK
                     LSFVQDGRQQTISLGRYPDFSLADAREWREEVRRKRAHGENVVNKKVRADFAFEKVAR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERAKTGNMAALSPSELPRLIDFLE
                     QRNEFAVYAGRVRIHPVTRLCIYWLLLTMTRIQEAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMQWVLDQARALNVNGRFVFESVNFQGHINKESPRVAMQRAGLDTTAHG
                     LRSLARTYLREVLKVDSIMRKRK"
     gene            complement(9349..9438)
                     /locus_tag="WIB30_001059"
     tRNA            complement(9349..9438)
                     /locus_tag="WIB30_001059"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9402..9404),aa:Ser,seq:cga)
     gene            complement(9516..9606)
                     /locus_tag="WIB30_001060"
     tRNA            complement(9516..9606)
                     /locus_tag="WIB30_001060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9570..9572),aa:Ser,seq:tga)
     gene            complement(9689..10468)
                     /gene="yaaA"
                     /locus_tag="WIB30_001061"
     CDS             complement(9689..10468)
                     /gene="yaaA"
                     /locus_tag="WIB30_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ3797139.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESEILMQQLRELALQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(10631..11818)
                     /gene="dapC"
                     /locus_tag="WIB30_001062"
     CDS             complement(10631..11818)
                     /gene="dapC"
                     /locus_tag="WIB30_001062"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ3797140.1"
                     /translation="MNTLLKQLKPYPFARLHEAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVVDVATCVKAAETIVS
                     LYR"
     gene            complement(11894..12346)
                     /gene="dut"
                     /locus_tag="WIB30_001063"
     CDS             complement(11894..12346)
                     /gene="dut"
                     /locus_tag="WIB30_001063"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ3797141.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            12494..12919
                     /locus_tag="WIB30_001064"
                     /pseudo
     CDS             12494..12919
                     /locus_tag="WIB30_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
BASE COUNT         3596 a         3382 c         3033 g         3192 t
ORIGIN      
        1 taatacacta gtgttgcact tgaaatccga atccaagcaa tattaaaaat tatcgtcatc
       61 aatgcggctt ccccagcccg cggcgagctt tccacgacag gcttttttta tttgggttgg
      121 tcgtctggtt tctttgagtt caaaaacagg tcgggatact taagttttat tcgtgcaggt
      181 atcccgcgct tcgtccaatt gaaaacgcat tgagggctat tccctgttat tcgaccaact
      241 tccgcgtaac tgccgattga ttgcaacagg cgtttgtctt cattgactct tttatccata
      301 aataaaccaa atgtttaaat ctaatgctaa tattaaacac tatgtttaga taaaaatcaa
      361 gtcttgtgta acaacatttt gtttaaatat gggagaataa ttaagccaac cgcgaataag
      421 attaaaaatg acaatgcacg agacaactga cagacttttt gagatagcca aagagcaggg
      481 agttttaaag ccggctgaca tagcagagcg tctgaatatc agccaacagg ctttgaaaaa
      541 ctgggaaagt cgtggcatag cggcaaaggc gctgcctgaa gtagcaaaag cattcggtgt
      601 atctgaaaca tggctgagaa caggtgaagg cagccgaacc gcgcccgtcc ttattgaccc
      661 cgacctaccc cacgaagtca aagacatcca ccgcccgatg acgtggagca gcaacgaccc
      721 gctgcccgac gatgattatg ttttcgtccc ctacctcaaa gagagctgct tcaaaggcgg
      781 agtaggcacg tatgaaatcc ccgattacaa cggctaccgc ctgccgttcg gcaaatccac
      841 gcttaaacgc aaaggcatca atcccgacaa cgtgttttgc tgcaccctga ccggcgacag
      901 tatggaggaa aaaatcgcag aagacgcggc aattgccgta gatacgggcg aaaccgccat
      961 acgcgacggc aaaatctacg ccttcgccca ggacggtatg ttccgcgtga agtacctgat
     1021 acggcagcct ggcaacagcg ttctgatacg cagccacaac agcggtttct atggcgacga
     1081 aaacgccccc ttggacagcc tgaccgttat cggcagggta ttttggtgga gcgtgctgga
     1141 ttgaaaaatg ttgctattac gacgagataa tattgatagg gctttcaaga ttgtcaaaaa
     1201 taggcgtttt gattcccctt ggtggcctgg tgagtacgat gccggtatga atttccttgg
     1261 cgtacaagga gagttgaagg ttcacgagct gcatcacaga acagcaaccc tgtgctttga
     1321 gtggcttggc gaagtatctg cgccgcgcag aaaagagaac tacaaagacc tcaagccaaa
     1381 tgtattgtat gactttgatg gttccggtaa gcattttgcc aaccctgacg caaggtatct
     1441 attgcccgtc ggttcaagcg gcttaatctt aaaacatatc caaatcgatg acgaagacac
     1501 tctgttgcgg ctatggtgcg cacgaaatat cccaatgcca caccggctat ctaaaatccc
     1561 gatgcttcgg cagtattatc taagcaaggc atggcacgaa atttatgcta tcaaccaaca
     1621 cctaaggaaa acgaagctga tagttgatgt ggcatacgac cccacagatt aaacaacccg
     1681 cccgcattat gcgggctttt ttcatgcccc gccgaacctg aaaacaacac aaaaccgaca
     1741 aagccgcctt aataggcggt ttttttgcgc ctgcgaaatc tcaaaaataa attcttttaa
     1801 taatcaaaat gtttataaat cttaaacaaa aatcttaaac agattgttgc atattattaa
     1861 acgctatgtt taaatacacc catcgaaaca aacaacagcc acatggagta ccgacatgga
     1921 aaccatcacc atccctaaag aaaatttcga cgctttgcta aatctcgcac gaaccgccgt
     1981 cgccctgaac gaaaatttta ttccaaaaat aaattacgga gcatccacca taccagcagc
     2041 cggctttgcc gcacttaacg agtttggaat agctcgctcc accgtggaat ctttggaaat
     2101 tctcaaaatt aaataacctc ctcggtattg tcatcccgtg ataccggcaa cctaactaac
     2161 ccctctttaa aggaaaatca aaaatggaaa cccaagaaaa gaaatttgtt cctgcctcca
     2221 agcaaatacc gaccgtaaat agcggctggt tagaccgtga attaggcgaa gccatgagtg
     2281 aagcggtgcg tgcctgcctt gctcacggta aacagtcaga aattactgtc aagctcaaaa
     2341 tccaacccca aaacatccaa agcggaacgg taaaaatcag ccacgatgta gcaaccaaac
     2401 tgcccaaaga aaaacgcgaa ggcggcatcg tctttgctac acctgacggc aacatccaag
     2461 ccgacgatcc ggcacaagga aaattgaaac tcaaacaagt tcccaatacg tcaagcacgt
     2521 tgaaaatggt cagatccaac taactcaaac ccaaaaggaa atctaaaatg gaaacccaag
     2581 aaaacatgat taaaaccgcc ttacaagcag ctcaaaaacc tttttttgag tttgcaccga
     2641 acaatactct gcttgtattc acaccagacc aagacggtgg ctggagatac aaatcacacc
     2701 ccgaattgat gcaaaacccg taccgcaagt gcggcaaatt cctcatgcac gataccgcca
     2761 gtcttattaa gtttgtacaa aaacacaaac aggacggcac acaaatctac attgatgccg
     2821 atttcaaatc agggcgtatt gatgtcaccg ccgtcatcaa cggccatacg caacaagctc
     2881 cgggctggct cgatttttat gccgtataca ccccgcaaca cactacatcg gcatccaact
     2941 ggctgaacaa caatgcgcac cgcatgaatc agatggagtt ttcccatttc ctgaccaaca
     3001 atgcccgcaa catcgtatcc aaaaatcccg gtaacgaaaa ttccgtttac ccgaccgccg
     3061 ccgaagtatt ggatttcgcg ctcaatctcg agtacaccga aaaaaccacc ttcaaacaag
     3121 gctatcgtga acaggacggc agaatcaact ttaccttcca atccgaagac tcagggcaaa
     3181 cagagaaaaa cctcaaaatg tttgaacgtt tcggcatcga gttcaccccg catcaaggcg
     3241 gtgcatccta ctttgttgaa gccctgttga aattccgcat cgacaaaaac agcggcgcac
     3301 tcgtcctgtg gtacgaattg cagcaaatcg atgctgtaat cgaacaagcg gcaaaagaca
     3361 tttccgaagc cgtacaaaaa gccttccctg aaatcgacat ctatttcggc gtaatcgcct
     3421 aaaaaaaggt cgtctgaaat tgtgaacctt cagacgacct ccacaaaaac ccaactccga
     3481 aagaccccaa aatgaccatc acagaagaat actccatcga catccgcgta accgcagaac
     3541 aggggaaaaa cgactacggc taccccaccg aacgctacgg atgcgacatc atcaacacag
     3601 acggggagct gcttgtcggc atagagccag agtacaaaac cccctttgcc gccgtcagac
     3661 aagcgctcat ctgtctgaca aaagacaacc tcaaaaacgc tgcctgatat taagccgtta
     3721 ccccaataat gaggaatcaa aatgaaatac ttgatccgca ccgccttact cgcagtcgca
     3781 gccgccggca tctacgcctg ccaaccgcaa tccgaagccg cagtgcaagt caaggctgaa
     3841 aacagcctga ccgctatgcg cttagccgtc gccgacaaac aggcagagat tgacgggttg
     3901 aacgcccaaa tcgacgccga aatcagacaa cgcgaagccg aagaattgaa agactaccga
     3961 tggatacacg gcgacgcgga agtgccggag ctggaaaaat gaactttcag gaagaaaacg
     4021 agaacatcaa gaatgacata gagattaatc tcttgcattc cgctgaagcc gacagcgtgg
     4081 aggcaatgat ggacttggcg gtttacgggc tggcggcgat ggttgccaaa cacaacaaaa
     4141 cgcgcggcga gcagcttgtg ttacaggcga ttgccggtca tatgcggaag cttttggccg
     4201 cgcttcccga aaccgagtca ttggtgcaga ccgggaaggt ttaccgccga cttgaagatt
     4261 tgatttttaa aacgtatttg ccggagcagt aaagatgaac acgaatcaag atacgattcc
     4321 atttggcggc aaccttgtga tttgctgttc gacgggaaac ggcggggacg ggcggttttc
     4381 ttgtctgatt accgatcaga taccggattt aaatcttgtc agaagcgggc aggcgttccc
     4441 gatgttcatt tatgagtacg aaactagaga aaaaaggaaa gcctgatgga caccctatta
     4501 aaaatcatca tcgcgctgtc attttccggc gctgaggcat tggcggtatg gcttttcatc
     4561 acggccgccg atgccgtatt acgccgcttc ccgaaaccga gtcattacag gagagtaaaa
     4621 aaatgaacat caacgaactg ggcgcaagga tagaccgccc gaccatccgc gaactgattg
     4681 cctacgcaac ctgccgcaac cgccccattt caaactcgac actgctgcgt atggaaaaag
     4741 acggacggat accgtgccgt ctgaaaaccc cactcacatc ccccgtatgg gacacccgcg
     4801 aagttttgga agccctgggg ttacagcaat aaaaaaacgc ccgattaggg cgttttcaca
     4861 ttcagacggc ttttgtattc cctactgcat caaaccgacg acaggttgcg gatttcgggc
     4921 agcatcgggc ggatttttgc cgcgtgttcc gcgtcggcgt gtgcgtttaa ggcttcgagg
     4981 gcgtttgcgg cggctttgag gcggctgcgt gtttccgccc agaccgtcca catcgttacc
     5041 gcctgtttgc agccgagctg cttcagcggc agggaaacgt ctctgcccat ttggatggcc
     5101 cacgcgccgt atctgacggc aacggcgagg tcgtacaggg cgttgccgct gatgggcagg
     5161 gcaggttgcg gtgcgggcaa tggttcgcgg tcgaggactt cgccgtacag cacgccgttt
     5221 tcaactgcca cgcggcccat cacgcttaaa acttcgggga actgttcggc aggtacttct
     5281 ttgtagctgc atccgaactt gcttttgacg gcagaccaaa gggtaatggc gatacgcgcc
     5341 tgcgcttctt tgggtgcgga tttggtcagg gcgttgtgca gttttttgac ggcttcgatt
     5401 tgttcgccgg tcaagccgtc cggaagggcg cgggtgggtt tgggggtaac tgtttttatg
     5461 ccgtctgaaa ttttgccgtt aagtaaaatt tcaatctgct catcgcacca aaccgcaaat
     5521 ttcggattaa gccaacgggc aaagtgaata gcgagtttgg gatgcagcca tgtgccttgc
     5581 tcactgccac cacgcttcac gataactatt tgatttgctt ccgttaggat ttttctccta
     5641 acgcttaaat tctcagcaag tgcagagata tattgttgag tttgttcact ttttaggtag
     5701 tctttaggta acttgccaaa gtgagatgca atggcggttg catttaaaaa accgtcttga
     5761 cggaaagata caggggtgtt accaaaattg agaacagata cgttcatgat agtttcctag
     5821 tttagtttcg aaagacccaa atgggtggtc gggaggttcg aaaacctact agaaaaggcc
     5881 ggcgttattc cccttgcggg tattgtattc cgcaccctcc cgaccatagg aaactagttc
     5941 ggatatatcg aaacaaacaa ccaaacaaga aaacttatgg acgtaaaaaa ttcacgctga
     6001 cggggtgaat gccgttctag tagaggtttt cgacgcctcg tgtttcgtaa tatatccaat
     6061 ctttctattt tctgcaagca taaaaaagcc gccatgtggc ggatagggtc gcggtcttca
     6121 acagcatgta ggatttagga atgcccaaat tgggcgcagg ggtgttgaaa acacgcaaac
     6181 agacgcgccg ccagccttac gggtaggcgc acccccacat aggagtaaat cgatttgata
     6241 cgtatagacg taaaaaattc cactctatcg tgttggatat acgctgtttg tcaggtgttt
     6301 tcaagcaccg tgggaaatat tatatagagt tatggaaatg tgtcaagaga attagcccaa
     6361 atgggcggac gcgcggttaa gaacccacat aggagggcgg acttattccc ctttcgggtc
     6421 ttgtattcgt cgcccacgcg gtcatagaaa cttcctgcta tcgaaacaaa caacaaggaa
     6481 agaaaactat agacatgaaa aaatcacatt gacggagtga ttgccgctgt agtgtggttt
     6541 ctgacgccac gaacaggaat ataaaacaaa acccctgcac atgcaagggg tttcccaaaa
     6601 aaccgtaggc ggcaaactga aaggccgtct gaatttcaga cggcctatgt tgcggcggat
     6661 gttaagctat taagctattt ggttttcaga ggctttttct tccattttcg ggctacggct
     6721 tcaaattctt gaaaggccgt cggtgcgctc ctgatacggc gtatttcaaa aatgaagctg
     6781 cttaggttgt cccagtcacg taacagcatg gagtattcga aatctttata cattttctcg
     6841 tgcagtgctc cgcccaatac gcccgcacag acaaattcgc gctggtttaa aactgtcagt
     6901 atggcttcac ggtctttctt cttgtccggg gtatccgatg tataggtggc gagtatgcat
     6961 ccgtctgttt ttgccagccc gtttactatg gttatggctt cttgaagggc ggcattgtta
     7021 cgttcggcca tgatcatgtc gatggtggcg cgctttttgg cgttttctgt attcctcatg
     7081 atgccgtaag cggcaacaaa aacgccgata acagtcaata tcggcgttgc tatctgtatt
     7141 aggttgtcag tcattgccgt cccagccgtc tgaaaggcgg aaatcacggc tttccgaata
     7201 aatatgtccc attgtcatcc ccttaagctg atgttgaaaa taaatccttg cgtcggaata
     7261 tacggcattg cttgactatt ttcaagattc tgtacctcgt gaaccggact atacaaaaaa
     7321 accgccttcg tgtaaagcgg ttcttaaatc gcccgttaag gcggacacaa cgatagccaa
     7381 actggaacag gaggcggcaa aggcgggttt gtccaaaaca gccttgattg aaaggctgat
     7441 tggggatttg taggcgtttg cagatacgac aaggggcgca ttccgcgccc catcacacgc
     7501 acggacggct tgtcatcgcc tgtcgcccgt gcgcggctgc ccatatttca gaacagtctt
     7561 gcaagcccct ttaagggaac ggttttattc tagtacagtt tgaatgcctt ggcaacggct
     7621 gttttaatcg cctgaaaatc ctcttcactg attattggaa tgcagcggtc gcgccctttg
     7681 ggtttgtatc ggtctaatcg tgccaatccg actgttgccg tcatgtcgca ttttgctcaa
     7741 cattggatgt gcggcttgtc cggtaagggg tttccactca ttttgtggtg gtagtccgcc
     7801 aaagggacag gttctgtgct gcttaagggt acgaccgtta ccagtttgcc gttgtgcctg
     7861 tttcgcgcta tgacgacgac agggcgtttc ttgaccattt ccggttcttc ataaccgcga
     7921 aagtcgcaca tgataaccga acgttccctt ggttggaatt ttaaaggcat tagccgctcc
     7981 tgacgatgac gggctgctat tatagcagtt cttacacaaa aaaccgcctt tgtgtaaagc
     8041 ggttgcaaaa aagccttcca ataaaaatgc ggaaacggtt ttttatttgc gtttccgcat
     8101 aatactatcc accttcagaa cctcgcgcaa ataagtacgc gcaagcgagc gcaaaccgtg
     8161 cgccgtcgta tccagccccg cccgctgcat cgccacgcgc ggactttcct tattgatatg
     8221 cccttgaaaa ttcacacttt caaacacaaa ccgcccgttc acattcaacg cccgcgcctg
     8281 atccaacacc cactgcatcg cccgcgacag cggcacatca tgcccccgcc gctccttttt
     8341 ccgttcggcg gggatacgcc aaacctcccc gtccaactcc gaccactcca tcaacgccgc
     8401 ctcctgaatc cgcgtcattg tcaacagcag ccaatagata caaagccgcg ttacaggatg
     8461 gatacgcacc ctgcccgcat aaaccgcaaa ttcattgcgc tgctccaaaa aatcaatcag
     8521 gcgcggcaat tcagacggac tcaacgccgc catattcccc gttttcgccc gttcaaacac
     8581 acccgaaccg atttgcgcga cagggttgat ttccatcatt cccgaaccga ccgcaaacgc
     8641 aaacaccatc ttcagactgt ttttcgtttt gcgcaacgta tcaacgatac cgcgcgcctc
     8701 catcacacgc agacagccga ccacatccgc cgtcctgact tgacgaatat caagattgcc
     8761 gatagccgga aaaacccacc gttcaaaatt ccgcataacc tgtccggcat acttttcaga
     8821 ccgacccttc gaccaacgca caaaccaatc acgcgccacc ttctcaaaag caaaatccgc
     8881 ccgcaccttc ttattgacga cattttcccc gtgcgcccgt tttcggcgca cctcctcccg
     8941 ccattcccgc gcatcggcca gcgaaaaatc aggataccgc cccagcgaaa ttgtctgctg
     9001 ccttccatcc tgcacaaacg acagcttcca actccgcccg cccgtcgggt agatccacaa
     9061 agccaacccg ccgccgtccg acaacttata cagcttatcg cgcggcttcg catttttaac
     9121 ctgatttgcc gacagcggcg taatgatttt cgccattatg gtaatttccc acttatccaa
     9181 aaattaccat atacattacc ataaaaaaaa atgcaataag aagataccgc aagattgcaa
     9241 atgatttact ttgacgcgtt ttgatagggc aaaccgatga tttttatatg gtaattgaca
     9301 caacttgaac aaaaaagaac cgccctgatt cagggcggtt atgttttctg gcggaaacgg
     9361 tgggattcga acccacggag gatttgcacc ctcagcggat ttcgagtccg ctgcattcag
     9421 cctctctgcc acgtttccga taatgaagta aaaccaaata aaaatgccga tgctttatgc
     9481 tacttcattt ttatttggtt tttgacccca aggattggcg gaagcggtga gattcgaact
     9541 cacggagggc tatcaaccct cgacggtttt caagaccgtt gcattaaacc gctctgccac
     9601 gcttccgtct tcttgaagaa gaagaataat aatgaagttt atacattttg ccaagcactt
     9661 ttttcggaaa atacttaatt tattgttttc actttatttg ttccgaacgc ataaaaaccc
     9721 attcgctttc atttgaagcc gcgtcattga atgcgtagcc ttcgtaatta aaatttttca
     9781 gcatttcagg gtcggtaata tgatgttctg ccgcatagcg caccattaat ccgcgcgcac
     9841 gcttggcata gaaactgatg attttatatt taccgttttt ttcgtcttta aatatcgggg
     9901 taatcagccg cgcccgaagt gttttcgtgt tgacggactt gaaatattcc tgtgaggcaa
     9961 ggttgacaag cgtattgctg cctgcttggg caagcgtgtc atttaaaagg ttggtaatga
    10021 tgtcgcccca aaactcatac aaattcttgc cgcgcaaatt ggcaaatgcc gtccccattt
    10081 ccaaacgata gggctgtatc aggtctaacg ggcgaagaag accgtataga ccggacagca
    10141 ggcggacatg gttttgcaga tagcgtatct gtccaatatc caatgtgttt gcatccatac
    10201 cttcgtaaac atcgccgttg aacataaaga ccgcctgttt ggcgttttcc ggcgtaaacg
    10261 gcgtgtgcca ttctgcattg cgctgcgcgt ttaagagggc aattttgtcg gaaacgtgca
    10321 tcagttcggc aatctgttgc aacgcaagct cgcgcagctg ctgcattaga atttcggact
    10381 ctgccagcag gtcgggttgg gtaaactcgc tgacaggggc agggtctttt tcgttaaggt
    10441 ttttcgcagg ggaaaggaca aaaaacataa tctgctcggt attgtgtcaa acacggggat
    10501 tttaagttta aacgtgtttg cgggcaagcc gattagatta aaagtgaagt aaataccgtt
    10561 atagcaaatt caaccgtaaa atattgaaaa atgccgtctg aacaaaagtt cagacggcat
    10621 tttttattct ttaccgatat agggaaacaa tggtttccgc agctttgaca caagttgcga
    10681 catcgacaac caaagcgata cgcacataac cttccccggg attgccctgt tcggtatccc
    10741 gtgccaaaaa acgtccgggc aatacttgga tagcggcttt ttgccataaa ttgcgtgcaa
    10801 atgccaaatc gtcgccatcg gggactttca accagatgta aaacgaggca tccggtaatt
    10861 tgacgtcaaa tacctgttgc aaaataggaa taacgcgctc aaatttttcc tgatacaggc
    10921 ggcggttgtc gataacgtgc tgttcgtcat cccaggcggc aatacttgcg cgctgcacgg
    10981 gaatgctcat tgcactgccg tgataggttc tgtaaagcag aaagttttta agcagttcgg
    11041 catcgccggc gacaaaaccg gaacgcaggc ccggaacatt ggaccgcttg gacaaactgg
    11101 tgaacataag cagtttttgc ctgcttcgac ccaactgtgc agcggcttgc aggcagccca
    11161 aaggtttgtt gccgtcgaaa tagatttcgg aatagcattc atccgaggca ataatgaaac
    11221 catatttatc ctgtaaatca aaaacttctt tccatccgtc caaatccagc acgctgccgc
    11281 tggggttgtt gggcgagcag acgaacacca gtttggtgcg tttccaaacc tcttcggaaa
    11341 tactgcgcca atcggggttg aaagacggcg cggggcaatt ggcaaaatgg atttcaccgc
    11401 cgcccaaaag tgtcgcacct tcataaatct gataaaaggg attcgggctg acaattgcgg
    11461 gtttgatgcc gtctgaaaca gggttcaaca cggtttgaac aaaagaaaac aacgcctccc
    11521 tactgcctaa aaccggcaga atttcattat ccgcatccac tgtcaagcca tcgtaacggc
    11581 gttttaacca gtttgcacac gcctgacgca gttcaggcag accggccgtc agcggatatt
    11641 tttccaactc gtgcaatgag gcggtcagcg catccgtaat gactttcggt gtcggatgtt
    11701 tcggttcgcc gatgtgcagg gggacggctt ccatgccttc gggcgcggaa atgccctgca
    11761 tcgcctcgtg cagtcgggca aagggatagg gtttgagctg cttgagtaag gtattcatga
    11821 ttttctccgg ttatcggtat atcggaagat atgccgtctg aacctgacgt gcccttcaga
    11881 cggcatttta tattcaatgc aaacccgtac tgccgaagcc gccctcaccc cggctgcttc
    11941 cgacaaattc ctcgacacgt ttgaaacgcg cctgcacgat tggcacgata accatctgcg
    12001 cgatacgctc aaacggtttg acagtaaacg gctcgctgct tctgttccat aacgaaacct
    12061 tcaattcccc ttgataatcg gagtcaatca agccgaccaa attgcccaaa acaatgccgt
    12121 gtttatgccc caatccggaa cggggcagca aaacggcggc atatgcggga ttcgccaaat
    12181 aaattgccaa acctgtcggt acaagaaacg tttcacccgg ctgcaagacg acttcctcat
    12241 ccaaacaggc gcgcaagtcc aaaccggcag aacccgctgt cgcataggta gggataaaat
    12301 ccgccatcct ctcgtccaac actttcattt ctacttcaat attcattgcc ttgctcccac
    12361 atacggtcgg tctgtctaaa gatggtggat tatacacaag ccgtgtttac actgaaacac
    12421 agcaaatagg ttttgttttg aaatactccg tctgtgtata atgcccgacc ttcttacata
    12481 tcaatattat aatatgacac acataagctc gccccgtaac gaatttatac gcggcatcaa
    12541 agaaagttcg cccatactta tcggacttct gccttgggca ttaatactcg gtatgcaggg
    12601 cggacaaaaa ggcatgagct ggctggaaat gttgttgatg accagtatga acttcgccgg
    12661 cggctccgag tttgccacgg tcaacctgtg ggcggaatct ctgccgatac tgcttatcgc
    12721 caccgtaacc tttatgatta attcgcggca tatcctgatg ggggcggcac ttgccctgca
    12781 cctgaaagaa ataccgctga aaaaagccgt gcccgcactg ttttttatgt gtgatgaaag
    12841 ctgggcgatg gcattctccg aaatccaaaa acggaaagca gccggtttgc ccgcattcaa
    12901 tatgcctttt tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttagctcaaa
    12961 gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atctgtactg
    13021 tctgcagctt cgccgccttg tcctgatttt tgttaatcca ctatatgaat cgtcattccc
    13081 gcgcaggcgg gaatctagac attcaatgct aaggcaattt attgaaaatg actgaaactc
    13141 aaaaaactag attcccactt tcgtgggaat gacgtggtgc aggtttccgt acgaatggat
    13201 tcg
//
LOCUS       DAWHRA010000051        12939 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWHRA010000051 DAWHRA010000000
VERSION     DAWHRA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12939
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.051"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(16..1212)
                     /locus_tag="WIB30_001065"
     CDS             complement(16..1212)
                     /locus_tag="WIB30_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ3797142.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLCRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            1616..2116
                     /locus_tag="WIB30_001066"
     CDS             1616..2116
                     /locus_tag="WIB30_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797143.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            2201..2755
                     /locus_tag="WIB30_001067"
     CDS             2201..2755
                     /locus_tag="WIB30_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797144.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            2769..2969
                     /locus_tag="WIB30_001068"
     CDS             2769..2969
                     /locus_tag="WIB30_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797145.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(3206..4594)
                     /locus_tag="WIB30_001069"
     CDS             complement(3206..4594)
                     /locus_tag="WIB30_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ3797146.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
     gene            4922..5869
                     /locus_tag="WIB30_001070"
     CDS             4922..5869
                     /locus_tag="WIB30_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3797147.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(5922..6254)
                     /gene="trxA"
                     /locus_tag="WIB30_001071"
     CDS             complement(5922..6254)
                     /gene="trxA"
                     /locus_tag="WIB30_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ3797148.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            6552..7025
                     /locus_tag="WIB30_001072"
     CDS             6552..7025
                     /locus_tag="WIB30_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ3797149.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGATMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYVLTKEMVDDLNDHIHLENDILFARVLDS"
     gene            complement(7096..7917)
                     /gene="nadE"
                     /locus_tag="WIB30_001073"
     CDS             complement(7096..7917)
                     /gene="nadE"
                     /locus_tag="WIB30_001073"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ3797150.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGTHQTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            8114..9469
                     /locus_tag="WIB30_001074"
     CDS             8114..9469
                     /locus_tag="WIB30_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ3797151.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAVRLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIESCTVPVVSGVGHETDFTLADFVADMRAPTPTGAAELVSPNRQESLHRLAQ
                     AQCRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNVRT
                     HTARFERQTQALKHCRPDVSVCKKNIDRFQTALSHAFHQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            9762..11303
                     /locus_tag="WIB30_001075"
     CDS             9762..11303
                     /locus_tag="WIB30_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ3797152.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(11372..12421)
                     /locus_tag="WIB30_001076"
     CDS             complement(11372..12421)
                     /locus_tag="WIB30_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ3797153.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETGKTASQPY
                     GQKASDGIKPQNVPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRIGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(12430..12657)
                     /locus_tag="WIB30_001077"
     CDS             complement(12430..12657)
                     /locus_tag="WIB30_001077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797154.1"
                     /translation="MQIHLLLLLSFERFYSRTQTDFNRRTNFAPTPSRSDDFIRKTAA
                     SGTISNRPTEDGILSTRPAAHAAEEPPYQAS"
     gene            12646..>12939
                     /locus_tag="WIB30_001078"
     CDS             12646..>12939
                     /locus_tag="WIB30_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ3797155.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFW"
BASE COUNT         2876 a         3506 c         3516 g         3041 t
ORIGIN      
        1 gacggcattt gagttttacg gattgtctga tgtcgcggca aattccgtca gcgcgtgttc
       61 caaacgtcgc aaaccttccg ccatatcctc atcgttcaac agtagcgaag gcgcgaaacg
      121 caatacgtcc gcacccgcaa ccaaaatcat cacgccgtgt ttcaaggcgg cggcggtgat
      181 ttcggatgcg cgtccgcgat aggcttcgtc caacacgcag ccgagtagca gccccatccc
      241 gcgaacttgt gagaacaagc ccgttttcct gcacaaatcc agcaatgccg tctgaagttt
      301 ctgcccctgt tcacggacat ggtttaaagt ttcgggcgta ttgatgatgt cgaatgcgcg
      361 gctgccgacc gcacacgcca tcgggttgcc gccgaaagtc gagccgtgcg tgcccggttg
      421 gaaggcggcg gcaatctttt ctgtcgccag catcgcgccg atcggaaagc cgcagcccaa
      481 ggcttttgcc gaactcaaaa tatcgggaac aatgccgtaa tgttcatagg caaacagcct
      541 gcccgtatgc cccatcccgg tttgaacttc gtccaaaatc aacaacgcat tgtgccggtc
      601 gcacagacgg cgcgcggttt gcaaatattc cgcagtggcg ggcaggatgc cgctttcgcc
      661 ctgtatcggc tcgatgatga ccgcgcaggt ctgttcgccg acggcagctt ccagcgcggc
      721 aatatcgttg aacggaacgt gcgtaatgcc ttgcggcagg ggtgcataat ccttgctgta
      781 tttcggctga ccgccgacgg acacggtaaa cagcgtgcgt ccgtgaaaac tgttgataca
      841 ggcgacgatt tcgctttttc ctccgccgaa acggtcgcgg gcgtatttcc tcgccagctt
      901 caacgccgcc tcattcgctt ccgagcccga gttgcagaaa aaaaccttgt cggcaaaact
      961 gtttgcaacc aatttttgcg ccaattcctg cgctggacgc gtcgtataga tattggaaat
     1021 gtgccacagc ttgtgcatct gcgcgtttaa agcatcgaca agggcagggt ggcagtgtcc
     1081 cagcgcattg acggcgatac cgcctgaaaa atcaatatat tcacgccctt tcgtatccca
     1141 aacgcggctg cctgaagcgc gttcgggaat catcggggca aaggcgaaat tgggggtcag
     1201 atagttttgc ataattgctc ctgattgcgg ttttgctcgg gattatgcac cgtttttcaa
     1261 agcagataaa gggaaattgc cgacaatatc gggcagcggt aaaaataaac cgaaatattt
     1321 tcaatcggat agatgaaaaa tcaaaaaaat ttcgataaaa tgcttgaggc gcggcagcgt
     1381 tttgatttag aatctcccca atatttttat cttcagacgg catttcatca tgcaaggcaa
     1441 aaaatttaca accgcttatt gctattggta ccacacagac gtgcggtact tttttgcgct
     1501 tgcttgaaat ccatcccata cgcaaaacag gccgctcgaa aacggggcgg cttttttgtt
     1561 accttcacac aggtcgcccg attcggcaac acacgacaga atcaggaaaa tgaccatgaa
     1621 tgcaatgccc caaaccgccg cacaaaacga aaccctgcac acttgtagca ttattgtcga
     1681 tgatgcacag gatttttccc ccatgcttct cgatgcaggg cgggacgaca cacttatcag
     1741 cgaatcgatg attccgcaaa tccgcaccgt tgccgccctg atagccgccg aacggcacga
     1801 cttcagccgc agcagccccg ccgagtttac cgatgccgcc gatttttttg ccgcacggat
     1861 tctcgtattg ggcgtgcgac gcttccatct ggacgtatcg ctgcttcaga ttttgaaaac
     1921 cgccaacagc cgcgcccgcc gctttgccga aaaacaccgc ctgtttttca cacccgcaca
     1981 agccgaattg agcctgaaca aacacaaaaa ccgccgcctg ctgaccgtag aaaccgaaca
     2041 cgaagtcgaa aacaaaggca acccagtcgc caacagcctc gcatttgtcc gcaaattgca
     2101 caccctgccc ctgtaaacat cacgatttgc cggcagaatg cttgccaaaa ttcccgacgg
     2161 cgatacaata aaaccgattt tccacccaaa caggacacac atgaaaaaaa tcatcgcctc
     2221 cgcgcttatc gcaacattcg cactcgccgc ctgccaagac gacacgcagg cgcggctcga
     2281 acagcagcag aaacagattg aagccctgca acagcagctc gcacagcagg cagacgatac
     2341 ggtttaccaa ctgactcccg aagcagtcaa agacaccatt cctgccgaag cacaggcaaa
     2401 cggcaacaac gggcaacccg ttaccggtaa agacgggcag cagtatattt acgaccaatc
     2461 gacaggaagc tggctgctgc aaagcctggt cggcgcggcg gcaggcgcgt ttatcggcaa
     2521 cgcgctggca aacaaattca cacgggcagg caaccaagac agtcccgtcg cccggcgcgc
     2581 gcgtgcagcc taccatcagt ccgcacgccc caatgcgcgc accagcaggg atttgaacac
     2641 gcgcagcctc cgtgcaaaac aacaggcggc gcaggcgcag cgttaccgcc cgacaacgcg
     2701 cccgcccgcc aattaccgcc gccccgctat gcgcggtttc ggcaggaggc ggtaaacccg
     2761 gcgcgccaat gccgtctgaa gagctttcag acggcatttt tgcatttgtt agggacattg
     2821 ttatgttgcc gtttgatttt cagacggcat tttgtttcca agcgtttgat gtcgggatgg
     2881 caattctgat ttacgtgcgt tggcagggtg gcattgtcat gccgccgttg ggttttcaga
     2941 cggcatcata tcgaacagtc aagccgtagg gcgagcattc ttgcccaccc tgcatctgaa
     3001 acccttccgt gtcattcccg tgcaggaagg aatctagata tttgaattta cggaaatttt
     3061 caatcgttgc caaagcggaa aagggttttt attacagcct aatcctttgg gtatcaaagc
     3121 cgggcggtgc tgaaacattg caaaaatgcc gtctgaaact cgaaatcggt ttcagacggc
     3181 attggtttat gcggcaggtt ggggcttagc ggtctttgta acggcgttcg cctttgcctg
     3241 caaaagaatc gccggctttg cgtttgcctt tgaagccttc gccggttttt ttgaaaccgt
     3301 ctctcttaaa gccttctttc ttgaaacctt cgccgcgcgt tttgccgccg aagccttctt
     3361 tgctcggttt atgatcgccg cgccaaccgc cggatttacg atcgccccag ccgcctttgc
     3421 ctttcggctt gccgcctgcg gatttgcgtt tgcgggtcgg ttccatgcct tcgatggtca
     3481 gttcgggcag ttttcggtta atgtattttt cgattttgtg gactttgacg tattcgttca
     3541 cttcggcaaa cgtaatcgca atacccgtgc ggcctgcgcg gccggtgcgc ccgatgcggt
     3601 ggacgtagtc ttccgcctgt ttcggcaggt cgtagttgat aacgtgggta atggtcggta
     3661 cgtcgatacc gcgtgcggca acatcggtgg caaccaaaat tttgcagcgg cctttacgca
     3721 aatccatcag cgtgcggttg cgccagcctt gcggcatatc gccgtgcagg cagtttgcgg
     3781 cgaaaccttt ttcgtacagt tcatccgcaa tgacttcggt catggctttg gtggacgtga
     3841 aaatcacgca ttgatcgata ttggcatcgc gcaagatatg atcgagcagg cggtttttgt
     3901 ggcgcatatc gtcgcagtac agcagttgtt cttcgatttt gccttgatcg tccacgcgtt
     3961 cgacttcgat gatttcaggg tctttggtca gtttgcgcgc cagtttgccg accgcgccgt
     4021 cccaagtggc ggagaacaac aaagtctgac ggtcgctcgg cgttgcttcc acgatggttt
     4081 cgatgtcgtc gataaagccc atatccaaca tacggtcggc ttcgtccaaa atcagcactt
     4141 ccaaacgttc aaaatcaact ttgccgcttt gcatcaggtc catcagacgg cccggcgtgg
     4201 cgacaatcag atcgaccggt ttgctcaggg cacgggtttg gtagccgaaa gacgcgccgc
     4261 cgacgatgct gacggtgcgg aaccaacgca tatttttggc atacgccagc gcgtttttct
     4321 cgacttgagc cgccagttcg cgggtcgggg tcaacaccaa agcacgcggg cctttgcccg
     4381 gtttttcgct gcgtttggtc agtttttgca aagtcggtaa cagaaaggcg gcggttttgc
     4441 cggagccggt ttgcgccgaa gccatgatgt cgcggccttc caaagcaaac ggaatggctt
     4501 gcgcctgaat cggcgtcggg ctttcgtaac cctcgctgct gacggcggac aaaatgtttt
     4561 tatcaaggtt caaatcggca aatttaatag acatggctat ccttggaaga caacaacgcc
     4621 cgcgtccgaa aggttcagac ggcctgaagc agggaagggt aacgatacgg ggaatgtgaa
     4681 gttacagggt tgtcgcaagc ggcttgcttg tggtttacat cgacggcaac ctgcacagat
     4741 atgctttgca aaatcaaagc aacagaagaa acgcgcccgt aagggcggtt taggttggaa
     4801 ggcgatggct tcgtatgaca agataagcga cggatggtat agggtttgcc cgaaacggtc
     4861 aagcaatcgg gcgttaatta cagtaaaatt gccgttttcc actttattcc gcctgaaacc
     4921 aatgaccgac atcacaccgt ttgccaaccg cttgggcaaa aacatcaaac accttatgaa
     4981 atgggcgaaa cgcaacggca tcgaagcctg gcgcatttac gaccgcgaca tcccccaatt
     5041 cccctttgcc gccgatgttt acggcgatcg aatccacctt caggaatacg ataccggctg
     5101 gctgatgcgg cccgaagaat acgaagcgtg gcttgccgaa gtcttggaag ccgtcgcttt
     5161 cgttaccggt tttgcgcccg aacaaatccg cctcaaacgc cgcgaacgcc aaaaaggttt
     5221 gcagcagtac gagaaaaccg gcaaggcggg cgacgatttc gtcatcaccg aaaacggccg
     5281 caagttttgg gtcaaccttg ataagtattt ggatacgggg ctttttttgg atcatcgaaa
     5341 cacgcgcaaa aaagtcggcg aaacggcggc gggcaaacgt tttttaaacc tgttttccta
     5401 caccggcagc ttcaccgtct atgccgccac cggcggcgcg gcatccagcg aaaccgtcga
     5461 tttgtccaac acatatcttg attgggcgaa acgcaatttt gaattgaacg gcatcgatac
     5521 ggaacgacac aaaatcgtcc gcgccgacgt gttccaatat cttcagacgg catatgggga
     5581 aggcaggcgg ttcgacctca ttgtgatgga cccgcccagt ttttccaaca gcaagaaaat
     5641 gtccgacatc ctcgacatcc agcgcgacca caaaaagctg attgacggcg cggtgaagct
     5701 gctcgcttca gacggcattt tgtacttttc caacaacctg cgcagcttcg tcttagacga
     5761 cttggtatcg gaacaatacg ccgtcaaaga catttccaaa caatccgttc ccgaagattt
     5821 ccgcaacaaa aaaatccata ggtgctggga aatccgccac aaatcttaag ggtgcgaaaa
     5881 agccgccgga atcttccggc ggctttttcg attgcgcttt atcaggcgat agaggcttcg
     5941 acaaaggcgg tcagctgacc ttttgccaat gcgccgactt tggtggcgac gacttcgccg
     6001 tttttgaaca ccatcagggt cggaatgccg cgcacgccga aacgggacgg ggtggcttcg
     6061 ttgtcgtcga tgttgatttt gaccactttc agacggcctt caaattcggc ggcaatgtcg
     6121 tccaaaatcg gggcaatcat tttgcagggg ccgcaccacg gagcccaaaa gtccagcagg
     6181 acggggatat ctgcatttaa aacgtctttt tcaaaagcgg catcgctggt gtgtacaatc
     6241 aattcgctgc tcattatttt tccttttcgg ttgggattgg cacgatagcg tgcaggatag
     6301 ggcttgggca taaaaatttc aagtgggcgg gcgggataat agtttttctg taccgcccca
     6361 atcgcctttg caaattctaa aaaacggttc ggacagtatt ttatttctga aaattacttt
     6421 tattatcaaa atgtaaggaa tacgtttgag gctgtacaga agcgcgcggg cggtgtatgc
     6481 tccgtttgaa ttgattctta aagtatgaat gttcaaaacc aaacccgaaa ccctgtaaaa
     6541 ggaacactgc gatgactgat ttttccgttt gggagaccgc cccctttggt gcaaccgtcg
     6601 atcatatcct gcaacgctac cacaatgtcc accgtgcaca atttgaagaa ttggtgccgc
     6661 tggcgcaaaa agtggcccaa gttcatgccg acacctttcc ggcggaaatt gccgagctgc
     6721 ttgcctatat gcagaacgaa ctgctgatgc atatgatgaa agaggaaagg atgctgtttc
     6781 cgatgattaa tcagggtgtc ggccggggtg cgacgatgcc catcagcgtg atgatgcacg
     6841 aacatgaaga acacgaccgc gccatcgcac ggctcaaaga gctgaccgat aatttccaag
     6901 cgcccgaagg agcctgcgga agctggacac ggctttacgt tttgaccaaa gaaatggtgg
     6961 acgacttgaa cgaccacatt catttggaaa acgatattct gtttgcccgc gtactcgatt
     7021 cttaaggaac gggagccgga tatttccatg ccgtctgaaa tacggttttt ccgcttcaga
     7081 cggcatttcc acgtttcagc ccaacaattc gggcggaatg cggcacacgg gaatcgggtt
     7141 gattttgtgc tgcatcgcgc ggtgcagccg cgtatagatt tccagaactt cgcgctgccg
     7201 cccttcaaaa tcttcgggtt tgcgcgtgcc gtacacgccc attgcccact ccagttcggg
     7261 atagcttgcg cccatctgtt cttcgtcggt gcgttccgta tcccacaggc cgtcggtcgg
     7321 cggggctttt tgaatcgcct cgtccacgcc caatgcttcg gcaagccggt aaacctgcgt
     7381 tttggtcagg tcggcaatcg ggctgatgtc cacgccgccg tcgccgtatt tagtaaaaaa
     7441 gcccacgccg aaatcttcaa tcttattacc tgtccccgta accagcagtc cgtgtatctg
     7501 cccgtagtag tacagggtca gcatacgcag gcggcttctg gcgttggcga gggaaagcgg
     7561 ctgattggca aatgccgtct gatgagtgcc gacggtttgt tcaaaggtct ggaaggtgtc
     7621 ggtcagatcg accgtttgcg cgcttacatt ggcatattgc cgttgcagat tgcggatgtg
     7681 cagccttgcc cgctcaagct ggccggggtg ttggcgtatc ggcatatcca gaagcagcgt
     7741 ggggcggccg gtgcgggcgg cgagtgcgga gacgacggcg gaatcgatgc cgccggaaac
     7801 gcccacgaca aacccttttg catttgcccg ggcggcgtat tcgtccaacc atcgggcgat
     7861 atgtgtgata actgcctgcg tatccatttt tcctcctgtc gaaaatatcc gcatcatagc
     7921 ctgccggcgg tgcaatttga atagattgca ggcaggttga tgaatatttt tcccattccc
     7981 cgcttaaaaa agtcatacgg gaaaaggcgg gcggcaagat ggcgcgtgtt ataatattgt
     8041 tgacaattta ggcggcccgg tgccgcctta ggcttcaaaa catattcata ttttttacta
     8101 tttgattgat tcgatgtctg atttttttca ttctgacgtg ttgtccgttt ccgagttgaa
     8161 cgcatttgcc aaaagtcttt tggaaaacca ccttgccggt ttgtggattg ccggcgaagt
     8221 atccaacctg acccgtgccg ccagcgggca ttattatttc tcgctcaaag acagccgcgc
     8281 acaggtgcgt tgcgcgatgt tcaagggtgc ggcggtgcgt ttggcgaagc ctttgaaaga
     8341 aggcgaccat atcgaggtat caggaaaaat cagtatttat gaagcgcggg gcgaatttca
     8401 gattaccgta aacgaagtac ggctcaaagg tttggggcag ctttacgaag cctatgagcg
     8461 gttgaaggcg cagttgcagg cggaaggcgc gtttgcggcg gaacgcaaga aacctttgcc
     8521 cgtccgtccg caatgtatcg gcatcgtaac cagtctggcg gcggcggctt tgcgcgatgt
     8581 cgttactacc ttgaaacgcc gcgcacccga aatccccgtt atcgtttatc cgacacccgt
     8641 tcaaggcgca ggcagcgaat tgcaaattgc ccaagcgatt aaaaccgcct cgcaacgcgc
     8701 cgaatgcgat gtgttgattg tctgtcgcgg cggcggcagc attgaagact tgtgggcgtt
     8761 taacgaagaa ccggtcgtgc gcgccatcga atcctgcacg gttccggtcg tcagcggcgt
     8821 aggccacgaa accgatttca cgctcgccga tttcgtcgcc gatatgcgcg cgcccacgcc
     8881 gaccggtgcg gcggaactgg tcagccccaa ccgccaagaa tcgctgcacc gcctcgccca
     8941 agcccaatgc cgtctgaaaa ccgttttgga acaacgctat ttcgatgcca gccaaaaact
     9001 cgactggctg gcgcggcaaa tccgtcatcc gcgccaaaaa ctcgacgaac agcgcgcttc
     9061 aatcggcaag ctggcgcaaa cgctgtctta ctcgatgacg caaaacgtcc gtacccacac
     9121 cgcccgtttc gaacgccaaa cccaagccct gaaacattgc cgtcctgatg tttccgtttg
     9181 caaaaaaaac atcgaccgct ttcagacggc cctgtcgcac gccttccacc aactgcttac
     9241 ccaccgccgc caaagcctga ctgcccaagc cgcattgctc gaagccgttt cgccgcagca
     9301 cattctggag cgcggctttt ccgtcgtcaa aaacacacgc ggacaagtca tccgcaacgc
     9361 cgatgttttg aagcaggggc agaagctgca cattatcttc gccgacggcg aaaccgacgt
     9421 gcgcgtaagc aaagaacaag ggcagcagga tttgttcgat tgcatttaaa cccaatcgga
     9481 aaaaccgctc ccccgccgcc gtcattcccg cgaaagcggg aatccagtcc cttgacctta
     9541 agataatctt taattcgtca ttcctgaaaa agcgggaatc cggaagcgaa aagccgcagg
     9601 aatttgtcgg aaaaaacaga aacctttccg tacacccgaa atgtttcaga cggcgcatct
     9661 tggggcggat aaaaacccga cccgatattt ttttataatt ttcaataagt ttccattggg
     9721 caaatgaaat tttatttttc aactgacaga aagaggatgt tatgaagttt ttagaccgtg
     9781 aggcaagcat tgccaagccg ggtttcaacc gttggctcgt accgcccgca gcattggcgg
     9841 tacaccttgc catcgggcag atttacgcct attccgtatt caacgcgccg ctgaccaaac
     9901 ttatcggcat aaccgaatcg gcggccggag attggaagct gacgaccgta ggttggattt
     9961 tcagtatcgc actcgcgatg ctgggcgcgt cggccgccct gttcggtacg tggatggaac
    10021 gggtagggcc gcgcaaagcc atatttgccg ccgcctgctg cttcagcctg ggcttcttcg
    10081 tatccgcatt cggcgtgcgt acgcacaacc tcttcctgct ctatttgggc aacggcgtca
    10141 tcggcggcgt aggcttgggt ttgggctaca tcgggccggt ttccacactg atgaaatggt
    10201 ttcccgacaa gccgggtatg gcgaccggtt tggcgattat gggtttcggt ggcggcgcaa
    10261 tgctggcctc gccgctttcc gtatcactga tgaacacctt ttcaaacgct gcttcggtcg
    10321 gggttgccga aaccttcgcc gtattgggtc tgctttacct cgcactgatg atgttcggcg
    10381 cgtttaccat ccgcgtacct gccgacggct ggaaacctga aggctatacc gcgccgaaaa
    10441 cgcaaaacaa gctggtcagc agcaatcatg tcaacgtgtc ccaagccatg aaaacgccgc
    10501 agttttggct gttgttctgg gtattgtgcc tgaacgtaac tgccggcatc ggcgtattgg
    10561 gtcaggcatc cgtgatgatt caggaactct tttccgaaac ctccataggc aggcaggcag
    10621 cagtcggcgc aggtgcggcg gcaggcttcg tcagcctcct gagcctgttt aacatgggcg
    10681 gacgcttttt gtggtcgagc gtttccgaca aaatcggacg caaaaacacc tacaccatct
    10741 tcttcgtact cggttcgctg ctgtatttcg ccgttccctc catcggcgag ggcggcagca
    10801 aagccctgtt catcatcggc ttttgcgtga ttatttccat gtacggcggc ggatttgccg
    10861 ccattcccgc ctacctgaaa gacctgttcg gcacctatca ggtcggcgcg attcacggac
    10921 gcatcctgct ggcctggtca accgccgccg tcatcggccc ggtactggtc aattacatac
    10981 gccaaagcca aatcgacagc ggcataccgg cggcacaggc atacagcgta accatgtaca
    11041 tcatggcgtg cctgctgatt atcggtttgt tgtgcaacct cgccgtcaaa tccgtacacg
    11101 aaaaacacca cgaaaaagac atcaaaaccg ccgcacacag cggcaacccc gatgacgaaa
    11161 ccgccatatc cgacgcttac cttgtgggcg aaaaagtttc cggcggcggc atatcggtct
    11221 ggtggcgttg ggcattggcc gtcataccgc tcgcctacgg cgtggtgatg gtctttgtca
    11281 aagcactcga ccttttctcc tgaaaacgcc cgaataaaat gccgtctgaa acagcagatg
    11341 tttcagacgg catttttgtg ttttggcggg tttaggcttt tttgcggcgg cgttcgcccg
    11401 gcagcagcat gtccgcccat ttgatgatgt tggcgacttc ggcggggttg agttcgtaga
    11461 actgcccgcg tttgaggcgg ttgggcagtc cgatgggacc gaagccgatg cgcacgaggc
    11521 ggctgacggt gagtccttgg ctttcaaaaa tgcggcgcac ttcgcggttg cggccttctt
    11581 taatcacgac gttgtaccat ttgttcgcgc cttcgccgcc ttgttcgcgg atgcgttcga
    11641 cttttgccaa gccgtcttcg agcatcacgc cttcttcggt gaggacgcgc atttgttcgc
    11701 cggtcagccc gcccaagacg cgcacggcgt attcgcgttc gacttcgaag ctggggtggg
    11761 cgaaacgttg gacgagttcg ccggaggtgg taagaatcag aagtccgctg gtgttgatgt
    11821 ccaagcgtcc gatggcgacc cagcggctgc tggcggcctg cggcaggcgg tcgaatatgc
    11881 tgacgcggcc ttgcgggtcg tcacgggaaa cgatttcgcc ttcttgtttg taatacagga
    11941 tgatgcgcgg caggcggtcc gcccatttga gcttgatgat gctgcctttg acggtaacgt
    12001 ggtcgtcggg ggtaactttg tcgcccagtt gcgcggtttt gccgttgacc gttatccagc
    12061 cgttggtaat ccattcttcc atttcgcggc gcgagccgac gccggacgcg gcaagcactt
    12121 tttgcaggcg ttcgggttcc atgcgcgaca ggtcgctgcg gcgttctttc aaatcgcgcg
    12181 cgtgttccat aattttttgg ttgggattgc ggacgacgag ttttttggct ttggcggcgc
    12241 gctgtttggg gacgttttga ggtttgatgc cgtctgaagc tttttgtccg taaggttggg
    12301 aagcggtttt gcccgtttca tctttgggac gggctttgct tttgaacggt ttggcggttt
    12361 tcttggcaga cggggctgcg ccgtcgcgcc attggcgttt gctggtgggc tgtttggaca
    12421 tcatttttcc taactcgcct gataaggcgg ttcttctgcg gcgtgtgcgg caggacgggt
    12481 tgataaaatg ccgtcctcgg tcggacggtt cgatattgta cctgatgcgg cggttttgcg
    12541 gatgaaatcg tcgctgcggc ttggggtcgg ggcaaagttt gtgcgacggt tgaaatcggt
    12601 ttgcgtgcgg gagtagaatc tttcaaatga taagaggagc agtaaatgga tttgcacaat
    12661 attcgggaag attacagcaa acgggaattg tcggaagccg attgcgccga taatccgatc
    12721 gagcagttcg agcggtggtt ggacgaggcg gtacgcgcac aggtcaacga gccgacggcg
    12781 gtcaatgtgg cggcggttga cggacgcggc aggcccaaca gccgtatggt gctgctgaag
    12841 gaagttaatt ctgaaggttt tgttttcttt acgaattatc acagccgcaa ggggcgttcg
    12901 ctggatgctc atccatttgc ggcgatgacg tttttttgg
//
LOCUS       DAWHRA010000052        12890 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWHRA010000052 DAWHRA010000000
VERSION     DAWHRA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12890
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.052"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(68..1378)
                     /locus_tag="WIB30_001079"
     CDS             complement(68..1378)
                     /locus_tag="WIB30_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ3797156.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(1517..2695)
                     /gene="ftsZ"
                     /locus_tag="WIB30_001080"
     CDS             complement(1517..2695)
                     /gene="ftsZ"
                     /locus_tag="WIB30_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ3797157.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2814..4058)
                     /gene="ftsA"
                     /locus_tag="WIB30_001081"
     CDS             complement(2814..4058)
                     /gene="ftsA"
                     /locus_tag="WIB30_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ3797158.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(4144..4872)
                     /locus_tag="WIB30_001082"
     CDS             complement(4144..4872)
                     /locus_tag="WIB30_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ3797159.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKTLGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(4862..5776)
                     /locus_tag="WIB30_001083"
     CDS             complement(4862..5776)
                     /locus_tag="WIB30_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ3797160.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(5889..7298)
                     /locus_tag="WIB30_001084"
     CDS             complement(5889..7298)
                     /locus_tag="WIB30_001084"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ3797161.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYLEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLALSKQI"
     gene            complement(7453..8520)
                     /gene="murG"
                     /locus_tag="WIB30_001085"
     CDS             complement(7453..8520)
                     /gene="murG"
                     /locus_tag="WIB30_001085"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ3797162.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGGLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8524..9849)
                     /locus_tag="WIB30_001086"
     CDS             complement(8524..9849)
                     /locus_tag="WIB30_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ3797163.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(9975..11312)
                     /locus_tag="WIB30_001087"
     CDS             complement(9975..11312)
                     /locus_tag="WIB30_001087"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ3797164.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRDDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIVTQDIPLQGLHN
                     AANVMAAVALCEAIGLSREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALVGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLGEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(11450..11668)
                     /locus_tag="WIB30_001088"
     CDS             complement(11450..11668)
                     /locus_tag="WIB30_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ3797165.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGHGVRQDRALAQEWLGKAC"
     gene            complement(11783..12865)
                     /locus_tag="WIB30_001089"
     CDS             complement(11783..12865)
                     /locus_tag="WIB30_001089"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ3797166.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         3062 a         3703 c         3252 g         2873 t
ORIGIN      
        1 ttaaatatat cggcaaacaa aatgccgtct gaaaaccttt cagacggcat ttttaatatc
       61 gaatcaatca gcccaaatac cagaatttca gtgcccacaa tacggcaaca atccatacca
      121 taggcggaac gtctttggtg cggcggcata aaagtttaac cacggcataa ctgatgaagc
      181 cgaaagcgat gccgtctgca atcgaataag taaacggcat gaaaacaatg gtcaggaacg
      241 caggtgcggc ttccgtcata tcgtcccaat caatatccct cgcactgcgg agcatctgcg
      301 tgccgacata aagcagggcg ggcgcggtgg caaaagcggg aacacttttc gccaaaggtg
      361 aaaacatcag gcaggcgagc atcaatacgc cgacggtaac cgccgtcagg ccggtccgtc
      421 cgcctgccga tacgcccgcc gcgctttcca cataaggcgt ggtggaagaa gtacccaaag
      481 ccgcacctgc cacaatggcg gtagagtctg caagcagtgc gcgtttcagg cggggcagct
      541 taccgtccac cagcagcccg gcacggtggg atatgccgac cagcgttccg gtactgtcaa
      601 atagatcgac caagaagaag acgaaaatca cactgaccat gctgacggta aacaggcctt
      661 caaaatccat ctgcataaaa gtcggcgcaa tgctcggtac ttcgccgatg atgccgtgaa
      721 attcattcaa acccatcagg ctggcaatga cggtaatggt caagatggtg atgatgattg
      781 cgccttgaac gcggaaatgt cccaatacga ccaccatagc aaaaccgaac aatgccaaca
      841 acgcggacgg ctgatgaata tcgcccaaac cgaccaaggt tgccggattg gcaacgataa
      901 tgcctgcgcc tttcagggaa atcagtgcca aaaacaaacc gataccggca gcaatcgaca
      961 ttttcaaacc cataggcagt gcgttgacca gcatttccct gactttaaaa aagctgaaca
     1021 ggataaaaat cagaccggag atgaacaccg cacccaacgc aacctgccaa ggcacgccca
     1081 tacccttaac gacggcaaag gtgaaatagg cattcagccc catccccggt gcgagtgcaa
     1141 tcggatagtt gccgacaaaa cccataacaa aacagccgat ggcagacgcg atacaggtag
     1201 cgacgaatac cgcccccata tccatgccgg tctcgcccaa aatcagaggg ttgacgataa
     1261 cgatgtagca catcgtcaaa aaagttgtca aacccgccat caactcggta cgcaccgtcg
     1321 taccgtttgc cttcagctta aaaatcccgt ccaacagtgt ttgttttgaa gtgtccatat
     1381 ctgaaagtct gtatcaagtg gaaaaatctg aatccgccga ccgcaaaccg tacgaaattg
     1441 caacatcttg aaaaacatac ctcccaaacc ggacaaaccg attcgggagg cagcaggttt
     1501 acgggcaaac agcacattat ttgtctgaat tgtgttgacg acgcaaaatc gcagggattt
     1561 caaagtcgtc aagtacggac tgattgtcga aatccgcagc ggtaaggttc atcgtgcgga
     1621 taccgcgatt ggtgcggatc ataccttcga cattgtggct ttgctcctgt ttggacggag
     1681 caaccgcttc tacctccctt gccggaacaa aatcgaccgc gcctttttct ttcagaccgg
     1741 tagcgataat ggtaatccgg atggcatctt cgctcatggt ctcgtcttca gccgcaccga
     1801 atttgcattc caaatcggga tgcgcgcttt ggttgacgat tttcatgact tcggacaact
     1861 cggacatttt caagcaaccc ggagcagtcg taatattgac cagcacaccg cgcgctccgt
     1921 ccaaggttac atcgtccagc agcggactgg aaatggcctg gtcggtcgcc atacgcgcac
     1981 ggtcgatacc ttgggcataa cccgaaccca tcatagcgat accgcggttg ctcatcacgg
     2041 ttttcacgtc ggcaaagtcg aggttgatga tttcgctcgg gcaagttacc acttcggaaa
     2101 tgcctgcgac cgcatcgcgc aatacattgt cggcggcacg gaaggcttcg cgcatcgtta
     2161 cgtcttcacc caatgcagtc atcagtttgt cgttcgggat gataatcagc gaatcgacgt
     2221 gttctttcaa ctgttccaac cctgcctgtg cgacatggac gcgcttacct tcatatgcga
     2281 acggtcgggt aaccacggca acggtcagaa tgcccaaaga cttggcaatc tcagcaacaa
     2341 ccggcgcgga accggtaccg gtaccgccgc ccataccggt cgtgataaac agcatattcg
     2401 caccgcgaat ggcttcttca atggcttccc ggtcttcctg ggctgccgca cggccgatat
     2461 cgggattcgc gcccgcgccc aaaccgcgtg tcagattcgt acccaactgg attctcttcg
     2521 ccgcatggtt ttttgccaga gactgcgcat ccgtattggc actgataaac tccacaccgc
     2581 gcacattgtt ggcaaccatg ttattgattg cattgcaacc gccgccgccc aagccgatta
     2641 ctttaatcac cgcagggctg actgccgatt ctgccacgtc gtaaacaaat tccattcaaa
     2701 aactcctgct cgccccattc agaggacggt ttaaataaat tattattcat tatataagaa
     2761 gtatcttgct gctggcaaaa tacttctcac ctgtcaaacg gcaatccacc tgttcagaag
     2821 ctgttttcaa tccaccgttt caatcttgcc aacaaaccgc cgcccccttc cctctcttgc
     2881 actgcaccgt tttccggctg cggcaagttt ccttccagct tgcatgctgc atgaagcagc
     2941 ccgatagcgg tagaaaaacg cggtgtgcgg acgcggtcgg acaaaccgcc catttcttgg
     3001 ggtgcaccgg tgcgtacagg caaatcgaag attttttcgg caaattccac aatcccggtc
     3061 atcatggaca caccgccggt cagaacgata cccgcattca gcacttcttt ggggaaaccc
     3121 gatttttgca gctcgcccag cactacgcca aaaatctcct gaatccgtgc actgatgatt
     3181 gctgccagaa ccttactgga aacctgacgc gatgtccggt cacccacgcc cggaacttca
     3241 atcatctcac ccaagccttc cgtatcgcat gatgccacgc cataatggat tttaatgtac
     3301 tcggcggcat cgagaggtgt tctcaacgat ttggacaaat ctttggtaat cagattacca
     3361 ccggccggaa tgacggacgt atggcggatg gcaccgttca tataaacggc aatatcggtc
     3421 gttccgccac caatgtcgat gacgcatacg ccgaggtctt tttcatcttc agtcagcacc
     3481 gcctgcccgc ttgccaacgg ctgaagcatg atctgatcgc ttttcaaacc gcaccgctcg
     3541 atacattttt ggacattctg cactgccgta cttgcaccgg taatgatgtg cacccgcgta
     3601 tccagacgca caccgctcat accgatgggc tccctcacgc caagttgggt gtcaataatg
     3661 tagtcttgaa ccacggcatc gagaattttt tgatcgggcg ggatattgat tgcctttgcc
     3721 gtttcaatgg cgcgatcgat gtctgcctgc gtgacttccc catctttaat tttaaccaca
     3781 ccttgcgaat tgagactgcg gatgtggttg cctgcgatac ctgtggtaac gtgagtaatt
     3841 ttggtatccg ccatcagctc ggcatcattg accgcctgcc tgatggcttg gacggtggca
     3901 tcgatattgg ttaccatgcc cgcgcgcaag ccccgtgaag gagcctgccc caaaccgacg
     3961 atgttgattt tgtcgtcatc ttgaacttcc ccgatcagtg cgaggacttt agacgtaccg
     4021 atatccagta cgctgatgta tctttgctgc tgttccattg ttcgtctgct cttaaaactg
     4081 attgaaattt gcgtcgcacc gtttcagacg gcacggccgt aatctgtccg atacctgttc
     4141 ccactattct tcggattctt tttcgggtaa accgtcggaa gcatagcgga ctgaaaatcc
     4201 gtccttatac ctcatatcca cataggataa ccgattttta tttttacgca acagatgctg
     4261 ccacgcttcg gtaaaaagcc ggaggcgttt catctcgttt tcccgtccga gcctgacggt
     4321 gatgccgttg tccaaaacga caatccacgc cgaacgtgcc gtataggtca tctctttgat
     4381 gcccaaaccc tgttttgcca aaacagtcga aaattcgtca taacggcgga gcatttcggc
     4441 agacgttcct tccgcgcctc tgaataccgg cattccgggt ctgtccaagc gggcttcaaa
     4501 aacattgcct tcgccgtcca ccaaggcatg gtcgccccaa cgcgcgaccg gcttgcgctc
     4561 ggtcaggacg acctcaaccg tgtcgggaaa acggcggcgc accatgaccg acgcaatcca
     4621 cggataccgg cggtaggcct cctgtgcgcc attgatgtcc gtcctcaaaa tattcccatg
     4681 gatgtattct ttcgccaaac tgcccaatgt cttcttatcg gaataaacca ggttgccctt
     4741 cagcgacacc tgcttgacgg gcagatgatt cgaattgtaa aaccaaacca gcccggacgc
     4801 agcaagcagc atcgccatca tgacaagcag ccagcgcgtc agccgttcca tcgcttcggc
     4861 attatcccac atgtgcggtc ttcaaaattt caatacataa atcggcaaaa cccacgcccg
     4921 taacggcagc ggatttcggt actaaactat ggctcgtcat accgggcagg gtgttgattt
     4981 ccaacagata gagtttgccg tcggtatctt tgaggaaatc gacgcgcacg cagccttccg
     5041 caccgattgc ctgcgcgccg cgaaccgcca gttcgcgcat caggctttct tcggcttcgg
     5101 tcaaatcttc cgaaggacat tgataaatgg tgtcgtcgcg gttgtacttg gcttcgtagt
     5161 cgtaaaactc ggttgcggga atgatgtgta tgccgggcag ccctttgccg ttcaggacgg
     5221 ggcaggaata ttcgccgccg ccgataaaac gttcggcaat gatttcgccc tgaaggtgtt
     5281 tcaattcttc gtaaacgctt ttcagacggc cttttccttt gacttttacc acgcctacgc
     5341 tgctgccttc ggccgccggt ttcacaaaca tcggcaggcc caatttttct tcgacggcat
     5401 cgaaatcagt gtcgtcgtgc aggacggcga actcgggaac gggcaatccc aatgcctgcc
     5461 aaatcagttt gcagcggtat ttgtccatgc cgatggcgga tgcggcgaca ccgctgccgg
     5521 tatagggaat gcccaacagt tccaatgcac cctgaaccgc cccgtcttcg ccgtaagtac
     5581 cgtgaaggat gttgaatgcc gtctgaaaac cttgtgcctt caattcagac aatggggttt
     5641 ctttaggatc gaaggcgtat gcgtctatgc ctttgctttt taaagcattc aaaatggcgg
     5701 tgccgctgtc cagcgagatt tctcgttcgc tggaaaaacc gcccatcaat acggccactt
     5761 tgccaaaatt ctgcattgtt tttgttcttt cctgattgct ttatgcttgt tgccagaggt
     5821 cgtctgaaac ctgatttgcg gtttcagacg atctttatat gatgttccgt ctgtcaggcg
     5881 ggtgtgcctc aaatctgttt cgacaatgcc agcagcgcgg cggggacgcg gttgatgctt
     5941 cccgcgccca tattcaacac gatgtcgccg tcctgcaaaa cgttcaacag catttcgggc
     6001 agatcggcaa cgttttcgca gtaaatcggc tcgagtttgc ccaacacgcg gatggcgcgg
     6061 gcaagagcgc gggaatcggc ggcggcaatc ggctcttcac cggcggcata aacttcggtc
     6121 agcaccagcg cgtcaacggt attgaggact ttggtaaagt cttcaaacaa atcgcgcgtg
     6181 cgggtatagc ggtgcggctg gaaggcgagt accaaacgtt tttccagata cgcgccgcgt
     6241 gcggcggcaa gggtcgccgc catttcgacg gggtggtgtc cgtagtcgtc caccaagagc
     6301 gcggtcccgc cgtttggcaa cttgatgtcg ccgtattttt ggaagcggcg gccgacgcct
     6361 tcaaagccga gcaagccttt ttggatcgct tcaaccgatg cgccgacttc cagcgccacg
     6421 ccgatggctg ccaatgcgtt cagcacgttg tgtctgccgg gcatattcag cacgacttca
     6481 aacgacccct gctcatgtcc tttcatttga acatggacgg tgaatttcat ttgcgcgccg
     6541 acgttttcga tgtcggtggc gtagatgtcg gcggtatcgt ccaaaccgta agtagcataa
     6601 ggtttgctca ctttgggcaa aatcgcgcgg acgtgttcgc tgtcaataca caaaaaggct
     6661 ttgccgtaga agggcatacg gtggatgaaa tcgataaacg cctgatgcag tttttcgacg
     6721 ctgtgcccgt aggtatccat atggtcttcg tcgatattgg taacgacgga cataatcggt
     6781 gtcaggtgca gaaaggatgc atccgactcg tcggcttcgg caacgatgta ttcgcctttg
     6841 cccaagcggg cgttggtgcc tgcggcgttg agtttgccgc cgataacgaa agtcgggtca
     6901 agtcctgccg cgccgaggat ggaggcggtc aggctggtgg tcgtggtttt gccgtgcgtg
     6961 ccggcaatgg cgatgccgtc acggaagcgc atcaactccg ccaacatcag ggcgcgcgga
     7021 ataacgggaa tttgctgctc caacgcagcg acaacttcgg gattttcttt tttgacggcg
     7081 gtagaggtaa cgacgacatc cgcaccgtta acgtgttcgg cggtatggcc gggataaact
     7141 tgaatgccca ggctgcccaa atgctcggta gcggcatttc gcgcctgatc cgaaccggaa
     7201 actttaaagc ccaaattgtg caagacttcg gcgatgccgc tcatgccgac gccgccgata
     7261 ccgacaaaat ggatgttggt aactcgattt ttcatcataa tgttgcgttc cggtggattt
     7321 tcgatgcgta aaggcgttat tttaaagggc tgaccgtttg cgcgccatag ttttctgaca
     7381 aatatatagc ggattgaaat aaaaaacatc catgccgtct gaatggcttt tcagacggca
     7441 tggttcggca gtttacgccg cacacgcaat cgcggcttcc gccacgtcgt ccgcactgtg
     7501 cggcagtgcc aacgtacggg cgttttctgc ccatttgagg catttttcgc ggtttaagcc
     7561 gccgagaatc tcggcgagtt tttccgccgt caactgggtt tgcggcaaca gcaatcccgc
     7621 ctccgcctgc accataaaac gcgcgttggc ggtttggtga tcgtcaaccg cgtgaggata
     7681 cggcactaac aacgcaccca atcccgccgc cgtcaactcg gcaatcgtca gcgcgccggc
     7741 acggcaaatc accaaatcgg catcgcggta ggcggacacc atgtcggtaa taaattccac
     7801 gcattcggct ttcacgccca gcgcgtcgta atccgcctgc aagctgccca gcttgccccg
     7861 tcccgattgg tggtacatct gcggacgcgc attgtcgggc agcaaagcca atgcctgcgg
     7921 tacggttttg ttcaaaacgt ccgcgcccaa actgccgccg accaccaaaa ttttcagacg
     7981 gccttcacgc ccttggaagc gttcggcagg cacgggcagg ttgctaatat cggcgcggac
     8041 ggggttgccg accaagccgc cttcgtggct gaacgctttc ggaaaagcgt acaacacccg
     8101 cttcgcccag cgcgacaggt ggcggttgga caaacctgcc acggcgtttt gctcgtgaat
     8161 cacaatcggc acgcctaata gcttcgccgc caaaccgccg gggaaggtaa cgaagccgcc
     8221 gaagccgatg acgcactcga cacggtgttt gcggataatc cgctgcgctt cgcggacggt
     8281 ttgatacaaa gtaaccggca gcatcagttt gcgtttgatg ccgttgccgc gcacgccttt
     8341 aatcgccagc gtttccaagc gtatgccgta ttgcggcacg atacgctctt ccatcgaatc
     8401 cttgctgccc agccaaatca catgatggcc gcgcgcgcgc aatgaatccg ccaccgccag
     8461 cgcggggaaa atatgtccgc ccgttccgcc cgccatcagc ataaaggttt taccgcccat
     8521 gatttactcc acccgataac cgcgcatttt ccggcggttt tcataatcta tacgcaacag
     8581 cagcatcatg ctgatcagca tgaaaaagac tgacgaaccg ccataggaca tcaacggcag
     8641 cgtcagacct ttggtcggca aagcaccgat gttcacaccg atattgaaga aactttggat
     8701 accgatccaa atgccgatac ccgaagcgat ataggcgttg aaagtcaaac ccaaatcgcg
     8761 cgactgcttg ccgatggaaa acgcccgcac caccagccag ccgtaacaga atatcagcac
     8821 gcacataccg aagaaaccga attcttcggc gatgatggca aaaataaaat cggtatgcgc
     8881 ttccggcaga aagccgcgtt tgctcaaact cgcacccaaa cccataccga accactctcc
     8941 gcgcccgatt gccatcagag agtgggtaag ctggtagccg gcaccctgcg ggtctttcca
     9001 cgggtccaaa aatgccacta cccgctgcac acggtaggga gcggcggtaa tcatcagcac
     9061 catcccgccc aagacgctgc ctaccaggac gaaaaaatat ttccacggca atcctgccaa
     9121 aaacagcatt ccaacggcaa tgacggtaat gacgacaaac gaaccgaaat ccggctgtac
     9181 cattatcagc accaaaccga acgccaccag cataatcggc aggatgatcg cccggaaacg
     9241 gccgtacatt tctaatgttt cacgacgtgc ctgcggattg gtggcggaca tgatcagatt
     9301 ggccgtcccc cgccaaatcg actgccaacc caaactttcc atgctgcgca acacttcttc
     9361 acggcgcgtg aacaggcttg ccaaataaag gatgaccgcc agcttgaaca gctcggtcgg
     9421 ctggaaattc aacggaccca aaggtatcca acgggtcgcg ccattgattt cgcgcccggc
     9481 aatcaatacg actaccagca acaggccgga tagggcaaaa atccacggca caagccgccg
     9541 ccatgtcctc atcctgcaaa gaaaccataa caaaccgctc gctatcaagc cggcaacgac
     9601 gaaccccgcc tgtctggtca aatagaaaaa ctgatcgccg ccttcttttg atgccaaata
     9661 cacagaagcc gaataaatca tcagcaggct gaacgccgtc atcagcacca ccatccacaa
     9721 aagcggcgcg tcgaatttcc tgccgtcgcg cacaatcggc ctgtcgagca gcagagtgtg
     9781 gacaccgtcg cccactttta ccaatacttc cgaaatcttc aaaaaaacca cctgccagtc
     9841 tgtttgcacc tgccgcaaag ggcaaaaatt tcagacggca gacaatgccg tccgaacata
     9901 cgatacatcc caaatcggta ttctaaatct ttacttgccg cccaacaatg acggcgtttg
     9961 catttcagac ggcatcacaa agccttaaac gcttcgataa acacttccga acggtgcgcg
    10021 tagcctttga acatatcaaa gctcgcgcag gcggggctga gcaacacaat atcgcctgct
    10081 tcggcttggg catatgccgt ctgaacggct tctcccaaag tggcgcagtc ggtcatattc
    10141 aagccgcagc cgtccaaatc gcggcggatt tgcggcgcat cgacaccaat caagaacacg
    10201 ccttttgcct tgcctaccag tgcatcgcgc aggggcgtga agtcctgccc tttacccatg
    10261 ccgcccaaaa tcacgaagag cggattttgc aaaccggcaa tcgcggcggc agtcgcgccg
    10321 acattcgtgc ctttgctgtc gtcgataaac accacgccgt ttttctcgcc gattttttcc
    10381 acgcggtgcg gcaggccttg gaaggttttg acgtgttcga gcaatgcttc gcgcgacaaa
    10441 ccgatggcct cacacaaagc cacggcagcc atgacgttag cggcgttgtg cagaccttgc
    10501 aacggaatgt cttgcgtgac aatcaaatct tcattgcctt gtttcaggcg gcctgtctcg
    10561 cgttccaacc agaaatcagc ttcgtgttcc aacgaaaacc attttacctc gcgcccggca
    10621 cgcttcatcg cgcggcagaa cgcatcgtcc gcattcaaaa cctgcacgcc gtcgccacgg
    10681 aaaatcttgg ctttggtatg cgcatagtcg agcaagtcgt cgtagcggtc gagatggtct
    10741 tcggaaatgt tcagcaccgt cgccgcagtc ggacgcaggc tttcggtgtt ttccagttgg
    10801 aagctggaaa gctccaacac ccacacgtcc gcctttttgc cttcgcgctg ccattccgcc
    10861 tccaaaaccg gcgtgccgat attgcccgcg ataacggtat ccagcccgca cttgatacag
    10921 agatagccga ccaggctcgt taccgtggtt ttgccgttgc tgccggtaat cgcaattacc
    10981 ttgtcgtccc ggcggttcac aatgtccgcc agcaattcga tgtcgcccaa cacgcgtccg
    11041 ccgttttgct tgaacgcctc aatatccggc tgccgctcgc tgatgccggg actgagagcc
    11101 agaatatcga aaccgttgtc cagcgcatct ttcagacggc ccgtgtaaaa caccaacccg
    11161 tcaaacatct taccgatttg cgacacgcgc tccggtttca gctccgcatc atacgcggca
    11221 acctccgcgc cgtttttgcg cagataggca atcatggaaa tacccgtacc gccgagtccg
    11281 gcgacgagga tttttttgtt ttgaaaagtc attttggttt gtcctaaaac aaatcatatt
    11341 gagcaggaga tgtccgcccc tgcccaagcc gctttcagac ggcatcgcga gctgttcaat
    11401 aacccgcctt caggcgttgg tcattgtcgc agccgtcttg gtttccgttt taacaagcct
    11461 tgccaagcca ttcttgtgca agggcgcggt cttggcgcac gccgtgccct ttgtaataca
    11521 ttgcgcccaa attgtattgg gctgctgcat ttccctgtgc tgccgcctgc aagttttccc
    11581 gaaaatccga tacgtcatcc gcccacaccg cttggttcaa gcccaaggca atcagggcgg
    11641 cggcaagcca tttgactgtc tgtttcatgg ttttacttct gttttagtat aagccgccgt
    11701 taacgataag gctgggcgga ttgtcgccgc aggtttattg cgcgttcaaa tgccgtctga
    11761 aagatgttca gacggcatag gttcagcgga ttttgagggt actcaaaccg atcaacacca
    11821 agacgatagt gataatccaa aagcggacga cgacttgggt ttctttccag cctttttgtt
    11881 cgtaatggtg gtggatgggc gccatcagga agatgcgttt tttggttttc ttgtaccagc
    11941 cgacttgaag cataacggat acggcttcta cgacaaataa tccgcccata atgacgagga
    12001 caaactcttg gcggacgatg acggcgacgg taccgagcgc ggcacccaat gccaatgcac
    12061 cgacatcgcc cataaagact tgcgcgggat aggcgttaaa ccacaagaaa ccgaggcacg
    12121 cgccgcacat ggcggtacag aaaatcacca cttcgtttgc gccggcaacg taaggtaatt
    12181 gcaggtattg ggcaaattgt gagtggccgc tggcataggc gaagatggcg aggccggcgg
    12241 caacgaggac gacggggaag gtcgcaaggc cgtccaagcc gtcggtgagg ttgacggcat
    12301 tggatgtgcc gacgatggtc aggtaagaca acaccaaaaa gccgaccacg cccagcggca
    12361 gggcgatttg tttgaagaac gggacaatca aaatattgtt ggcggaattg gcggcaaggt
    12421 aaaacaatgc caaaccggcg ataatggcaa cgcttgactg ccacaccatt ttgaatttgg
    12481 cggacacgcc gttggggtct ttatagacga ctttgcgcca gtcgtcgtaa aaaccgagcg
    12541 caccggtggc aagcagtacg cccaagagaa tccagatata cgggttcgcc cagttgcccc
    12601 acaacagggt ggacacggta atggcggtca gaatcagcga accgcccatc gtcggcgtgc
    12661 cgtttttgac gaggtgggtt tgcgggccgt cggtacgcac tgcctgcccg catttgagcg
    12721 cggtcagcct gcgtatcgtc cacgggccga acatcaggga aaacgctaag gcggtcaacg
    12781 ccgccatgac ggcgcggaat gtggtgtatt gaaaaatatt cagaccggtt aaccagttgc
    12841 tgaaatgtgc gagccataaa aacatggggc ttcctttttt tgttttgtcg
//
LOCUS       DAWHRA010000053        12804 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWHRA010000053 DAWHRA010000000
VERSION     DAWHRA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12804
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.053"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..443)
                     /locus_tag="WIB30_001090"
     CDS             complement(<1..443)
                     /locus_tag="WIB30_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ3797167.1"
                     /translation="MNEILRQPSVLLFLTLAVYAIAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPA"
     gene            complement(440..787)
                     /locus_tag="WIB30_001091"
     CDS             complement(440..787)
                     /locus_tag="WIB30_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ3797168.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRGIIR"
     gene            complement(932..1150)
                     /locus_tag="WIB30_001092"
     CDS             complement(932..1150)
                     /locus_tag="WIB30_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797169.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            complement(1169..1924)
                     /locus_tag="WIB30_001093"
     CDS             complement(1169..1924)
                     /locus_tag="WIB30_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ3797170.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            2169..3077
                     /gene="hslO"
                     /locus_tag="WIB30_001094"
     CDS             2169..3077
                     /gene="hslO"
                     /locus_tag="WIB30_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ3797171.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIGEDESLVDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            complement(3163..4617)
                     /gene="mgtE"
                     /locus_tag="WIB30_001095"
     CDS             complement(3163..4617)
                     /gene="mgtE"
                     /locus_tag="WIB30_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ3797172.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(4849..9141)
                     /gene="mspA"
                     /locus_tag="WIB30_001096"
                     /gene_synonym="ausI"
     CDS             complement(4849..9141)
                     /gene="mspA"
                     /locus_tag="WIB30_001096"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ3797173.1"
                     /translation="MRFTHTIPFYSVLSTLGLFAVSPAYSSIVRSDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLSGIPMPDFRVSNRQTAIATLVHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTQTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGDSLTDQPLNTYAVAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDSFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGDSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMKNSTSWQFIGNNRQQAAEQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMNLN
                     GEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAGRN
                     TAHLDGDITAYDLSGIDLGFTQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPATQV
                     RGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQIT
                     LNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPLKLEGDSRGAFQIHVK
                     NTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNPLKE
                     AELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARIDSQ
                     VQYLSAQLKQTDPLTGILTRAQNLCAAQGYSADICRQVAKAADTNDLTLFETELDTYI
                     ERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNLAEL
                     ISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHRPYQ
                     QTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENGALF
                     AAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSNGNR
                     YVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSALSVND
                     RTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            complement(9448..10206)
                     /gene="gloB"
                     /locus_tag="WIB30_001097"
     CDS             complement(9448..10206)
                     /gene="gloB"
                     /locus_tag="WIB30_001097"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ3797174.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWHGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            complement(10301..>12804)
                     /gene="purL"
                     /locus_tag="WIB30_001098"
     CDS             complement(10301..>12804)
                     /gene="purL"
                     /locus_tag="WIB30_001098"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ3797175.1"
                     /translation="CWQLGGKNPIISIHDVGAGGLSNAFPELVNDAGRGAVFELREVP
                     LEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAICERERCPFAVVGTATDDGHLKVR
                     DDLFANNPIDLPLNVLLGKPPKTTRTDKTVTPSKKPFHADDIDITEAAYRVLRLPAVA
                     AKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAVTMMGFNTYRGEAMSMGEKPAVAL
                     FDAPASGRMCVGEAITNIVAANIGDIGNIKLSANWMAACGNEGEDEKLYRTVEAVSKA
                     CQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLSLIISAFAPVEDVRKTVTPELKNV
                     EDSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPDLDDTSRLKAFYNVIQQLVAEDKL
                     LAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLAQTFITNHTALSQSLRTEEVKALA
                     EWQETIARTLFNEELGAVIQVRKQDVADIINLFYQQQLHHNVFEIGTLTDENTLIIRD
                     GQTHLISDNLIKLQQTWQETSHQIQRLRDNPACADSEFALIGDNERSALFADVKFDVN
                     EDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRAGFDAYDVHMSDLMAGRFRLAD
                     FKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFAAFFADPDTLTLGVCNGCQMVS
                     NLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKSASLILNEMQGSSLPVVVSHGEGR
                     ADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNGSPQGIAGITNADGRVTIMMPH
                     PERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG"
BASE COUNT         2475 a         3156 c         3994 g         3179 t
ORIGIN      
        1 gcgggaatgc cgccgatgga gcgggtgatt tcaatggcga tggggttggt aacagatttg
       61 gacgcgagcg agaggacgac ttcgcgttcc gcgcccagcc atttggcaaa atacatcccc
      121 gtaacaatgc ccgtaacgct gcccgcaagc tgtgaaacga tgacgggcag ccactggttg
      181 aagattttac ggcggttttg gtagagcggc acggcaagca cgacgacggc gggttttagc
      241 caaaaatcaa tgaattgcgc ggcgttgtgg tacaccgcat aatcgatacc gaggattttc
      301 aggtaggcaa tcagcacgat agtgctgacg agtacggggt tgcagaagat attacccgtg
      361 cgcgtgcgca cgataatcgc aatcgcgtac acggcaagcg tgaggaaaag cagaacgctg
      421 ggctgcctga ggatttcgtt catcggataa taccccgtat ccaccggtga accttgcctg
      481 taaccagcag tacgcacaaa gtgctggcgg aggcggaaac cagtatcgaa aaccaatcgt
      541 cggcaatcaa atccaaatag ctgataaccg ccacgcaggg cggcacgaga aacagcgtca
      601 gattcgccat cagcgcgtcg gtaagctgtt gcagccaaga cgttttgacc caacccgcct
      661 gcaaaagcgc aaacagcacg cccatgccga cgatgctgcc gggcagtttg atgcctgcta
      721 ggaaaacggc ggtttcgccg gcggcgaggc agccgaggat gatgaggagc gcgcggatga
      781 tgttcatgtt gtgtgtctgt cttttgacgg cgttgtgata aatcttgtta actgtttaat
      841 cataacgtca aatatgccgt ctgaaaagcc gaagccgcgc acaaacgcaa aaacggcacg
      901 gataaaccgt gccgcagcaa ccggggcgtg ttcagacttt gcggtaggga ttttccaaac
      961 cttcaatcag cgcgagcagg taatcgcgca actgacgttc gccgcaaccc atcagcagcg
     1021 cgtcttcaaa cgcgtcttgt gccgtctgat acagctcggc catattttcg gacatcactt
     1081 tgaccttttc ggtacaggac acgatctgtc cgtcatcgtc gtaccatttg ggcatttcgg
     1141 gcatactcat tgcgtattcc ttgggaaatc agttcagaaa gcgggacggg tcgtttttct
     1201 tgacccggcg ggcgaacgcg tatttgccgc tccaatattt gtggctcagg ctggtgattt
     1261 cgatattttt ccccgtgcgc ggcgcgtgga tgaagcggtt gttgccgata taaagtccga
     1321 catgggaaat gcggctgccg ccgagcgtgc ggaaaaacac catatccccg ggctgcaatt
     1381 cgcttcgggc aaccggcgta cccatccgcg cctgttctgc cgacgtgcgc ggcaggttga
     1441 tgcccatggc gcgtttaaag atgtgctgca tgaagccgct gcagtcaaaa ccggtagaaa
     1501 ccgatgtgcc gccgtagcgg taggcaatac ccaaaagtcc catcgcgttg ccgatgagtt
     1561 cgtcggcatt gcccgcccgc cgagcggggg ctcggttgac gggtaaaacg ggctgttcgt
     1621 taagccccat cgcgttgccg atgagttcgt cggcattgcc cgcccgccgg gcggggactc
     1681 ggttgacggg taaaacgggc tgttcgttaa gccccatcgc gctgccgatg agttcgtcgg
     1741 cattgcccgc ccgccgggcg ggggctcggt tgatgggtaa aacgggctgt tcgtcttcgg
     1801 caaactgtct gagaatctgc tcgcggctgc tgagcaggtt ggtcaactcg tcggcaaggg
     1861 cggggcggac ggcaaacatc agccacaaaa ccgcccaaac tgccggtttg aaaaaagaat
     1921 ccatatcggt gtttccgcgc aggaggcatc gtgccgccgt gcgatgttat gctgtatcag
     1981 tcgaaaatat cttgttgatt gtatataaaa aaacggctgt ttggcaaaaa acggcttgtt
     2041 agattgagtt aatatgttga tttttatata atttattgat aatttgggaa gcgcatccgc
     2101 cgtccgtctt gttttcgcgc cgaccgcaac catatagccg ccatccgaac aatcagtcag
     2161 agaaaatcat gaatcaaacc gccatcaacc gcgccgatgt gcgtacccgc tttatcttcg
     2221 acgatatgcc cgtgcgcggg ctgcacgtcc gtctggaaaa cgtgtggcag cacattgtaa
     2281 aacagaaaaa ctatcccgcc gccatccgcc gcgccttggg tgagctactg gctgccggcg
     2341 ttctgctgtc gggcaacctt aaaaacgaag gcacgctgat tgtgcaggtt caggggcagg
     2401 ggcggctgaa aatgctggtt gcagaagcga cttccgaccg taccgtccgt gcaaccgcgc
     2461 ggtgggacga ggcggcagaa atcggtgaag atgaaagttt ggtcgatctt ttgggcgagg
     2521 gcggcgtatt cgtgctgacg ctgcagccca aagacggcga accctggcag ggcgtagtgc
     2581 ctttggaagg cgacggtatc gcgcaaatgt tgatgaacta tatgaaacgt tccgaacagc
     2641 ttgatacgca catcgtcctg tctgcaagcg atgaagcggc gggcggtctg ctggtgcagc
     2701 gtctgcctga agaggtgttg gatgaagagg catgggaaca cgtcagtacg ctggcgcgca
     2761 cgctgacggc gggcgagttg gcagaattgg atgcgcaaca tgttttgtac cgcttgttcc
     2821 acgaaacgcc gccgcgcgtg ttcgagccgg aaacgtttga attttcatgc acctgttcgc
     2881 gcggcaaagt cagcgatatg ctgttgatgc tgggcgggga agaagtcggc ggcgtggtgg
     2941 cggaacaagg cagcatcgaa gtcgattgcg atttctgcca cagcaaatat gtgtttgacg
     3001 aaaccgatgt caacgcgctg ttcggggagg atgtggtcgg cgttgccaaa gggctgcccc
     3061 ggcataccgt ccaataatct tgtgcaacaa ggataaatag tcaaatgccg tctgaagcag
     3121 cttccgcttc agacggcatt tttgcgcggg ttcagacggc atttaaagca ggaatagggt
     3181 ggcgagcccc aagaaaatca ggaagccgcc ggagtcggta acggcggtaa tcagcaccga
     3241 gctgcccagt gcgggatcgc gtccgaactt ttccattacc acgggaatta atacgccgac
     3301 ggttgccgcc agcaggaggt tgagcgtcat cgcggcaatc ataaccagcc cgatgccgag
     3361 gctgccgtag agcagccatg aaaccgcgcc catgacggtt ccccagataa tgccgttgac
     3421 tagggcaacg ccgacttctt ttttcaacag cctgcctgcc tgcatgcctg tcagttgccc
     3481 catcgccatg gcgcggacaa tcatggtaat cgtctggttg cctgagttac cgccgatgcc
     3541 ggcgacgatg ggcatcagcg cggcgagtgc gacgattttt tcgatgctgc cttcaaacgc
     3601 gccgatgacg cggctggcga ggaaggcggt gcagaggttg acggcgagcc acatccagcg
     3661 gtttttgacg gaatccaaga cgggggcgaa taagtcttcc tcttcttgca aacccgccat
     3721 attcaacata tccgcttcgg attcttcgcg gatcacgtcc accatctcgt caacggtaat
     3781 cctaccgatg agctttttgt tttcatcgac aaccggcgca gttaccaagt cgtagcgttc
     3841 aaacgcctgc gccgcttctt ccacgtcgtc ttcggcgcgg aaacgcacga catctttcgc
     3901 catcacgttt tccaccaaat cttcgggatc ggcgaccaaa agtttgcgga tgggcagcac
     3961 gccttgcagt acgtcgtttt catcgaccac aaaaatcttg tcggtatggt cgggcaggct
     4021 gtcgaagcgg cgcagatagc gcagcaccac ttcacaggcg acatcggcgc ggatgctgac
     4081 caactcgaag tccataatcg caccgacttg gttgtcttcg taggacattg ccgctttgac
     4141 ttgggcgcgt tcttcctcat cgcgggtttg tagcgcttcg taaaccactt ggtgcggcaa
     4201 atcgtctgcc agttccgcca attcgtccgc gtccaaatca tcgaccgctg ccaacaattc
     4261 gtctttgtcc atcgactcga tcagcgtttc gcgcaccgcg tcggatactt ccagcaatac
     4321 ttcgccgtcg tcttccggtt tgaccagaat ccagacgata ttgcgttcgc gcggcggtag
     4381 cgattccaat accgccgcca cgtcggcagg gtgcagctcg ctcaagagga cggtcagctc
     4441 ggtcagcttg tcgcgcagcg gcgcgtcttc gagcggtgta ccgttttcga tttgttcaaa
     4501 agcaggttcg aggatttcgc agagggagtg gacacggtcg aaatcggcgg aaacgcgttc
     4561 tacatcgttt tcgataccgt cgttttcaag gttcggaggg gttggttcga tgctcataaa
     4621 tgctccgccc gccgtgtgcg gggaggcatt ccggcgggat ggttattgcg ggtttgaatc
     4681 ggatggggtg ttcagtaaaa acgaactggg aaggtccata ataaaagcct gacggtacag
     4741 gcgcagggtt ggaaacggca ctattctact cccttttgga acggtttact atttttaacg
     4801 caaatgccgt ctgaaacgcg ggttccagac ggcattttta tcggcttgtt accagttgta
     4861 gcctattttg attcctgcct gtttgtggcg ggcggtgttg ctgtctttgc cgtaagcggc
     4921 atgaagtttg gcgttccagc ctttgtatcc ggcgtcgatt tgcagggtat gcagtgtgcc
     4981 gtgggcggct tgctgcagta aggtacggtc gttgacgctg agggcggaac cgctgttttg
     5041 gcgggtatgg tagtaatcgc tgctgaaagc gggggtcagc ttggcttgac ccagttcgac
     5101 ggttttatcg agacggatgc cggcatgcca tgtggtttgg atttgcgccg ggctgtttat
     5161 ctctgcgccg tcgagtacgt accggttgcc gttgctgcgg tttatacgga tgccggcata
     5221 gggtctgagg ttgatgccgg tatcgatttt gatgccggtg ttgatgcctg catcccatgc
     5281 gtggcggcgg acggcagccc cgtcataatc ggtaaatcgg gtacggctgt tgctgtagcc
     5341 taaatctgcc gcggcaaaga gtgcgccgtt ttcccctttg acgaacagat gtgcgccgtt
     5401 gctgcggttt cgggcggata cgccttcatc aaaacggttg tttgtgcgct catcggttaa
     5461 aatcgtaccg acactgagac ggtcggtgat gccggtttgg atgccgatat gtgcatagtt
     5521 ggtagtttgt tggtagggac ggtgtgtgcc gctatggtag tcggtttgtt gcgtaccggt
     5581 ttccagccag atgttttgct gctgcggatc ggtaaggtgg cggtcgatgc ggcgtcccgc
     5641 ctgttgccgg ccggtattgt aggcggcctg ttcggaaacg gcggtgttgg ccgaccggct
     5701 gatcagttcg gccaggttcg gcatacggat gccggcaacg ccggttttca aactgtggat
     5761 ttgtcgggac agacggttga gtgcgttcag gtaggcgtgc cgggctgttt cgacggcttg
     5821 cgcatcgccg ccttgccgtg ctttgtcaag ttcggattcg gccatttcta cacgttctat
     5881 atacgtatcc agttcggttt cgaagagtgt caggtcgttc gtgtcggcgg ctttggcaac
     5941 ctgacggcag atatcggcac tgtatccttg tgcggcacac aggttttggg cacgcgtcag
     6001 aatgccggtc agcgggtctg tctgtttcaa ttgggcggac agatattgga cttggctgtc
     6061 gatgcgggca agttcggttt gactgttctg ccaggcctgt agtgcctgcc tggtcgcgtc
     6121 agagtcatgt tgaacctgtc tgctgatgtc ggttgcctgt aattggttgt atgcctgttg
     6181 gttgcgctca tgttccgcac gcgtggcttc aatttgaagt tcggcctctt tgagcgggtt
     6241 gtacaggctg tatccgttgt tgtttttgcg gaggatgtag cggtaggcac ccaaatcggc
     6301 atagccgttt tggagggtga atcgggcttg gtggctgtgt ttcggattga ggctcacaag
     6361 tgcaagcgat tcggttgttt gaggttcttg tccggtgttt ttgacgtgga tttggaatgc
     6421 gccgcggctg tccccttcca gtttgagggg ggcattttgt tttcggacga tgccggtcag
     6481 gaatcggaat gtgccgaacc cgtcaagtgt gccgttgacg gtcagtgtgt tgaagcggtt
     6541 gttgtgtgta ttattggcga aatcggggtt cagggtaatt tgtgcgccgt caagcgtcag
     6601 tgcgccggtg tggctggact gggaaagtgt ccagttgctg tctgcttcca tgcggactgc
     6661 ggtgtctttg ccggcacgga tgctgccgta caggtgtgct ttgcccaggc ggagctctga
     6721 acggtcgtta agggtaatgt cgccgcgtac ttgcgttgca ggtagtgcac gatagttttc
     6781 ggcttttaaa acggcgttgg gtgtgcagtg ggtgctgccg ctatggtagg agcggtagca
     6841 ttccggtgtt ttgccttggg taaagccgag gtcgatgccg gacagatcgt atgcggttat
     6901 gtcgccgtcc agatgcgcgg tattgcgccc tgccgtcagt cgggcatggt ttcgcagggt
     6961 gaaccgtgca gccttgaagc tgccgtcggt ccattcgttt tcaagaacgg gttcgcgttt
     7021 ggcctggtgg tcgtaggcat ggggtacggg cctgcctgac acaatcatat tgccgccctc
     7081 aatcaagact tccccgttaa ggttcatgcc gccgttgagc aacagcgtgc tgtcggtgcg
     7141 tttgggacga tagtgcaggt tgagtgcgcc gttgtaacgg tattcgggcc ggccgtatac
     7201 ggcggcattt gcgatatggc gggttttttc tatggctgct tcattggttt tgctgtaact
     7261 cggcgcggct ttgcccgttt gcgcgttttc acccaagtat ccgccgaagg taatcaggtc
     7321 ggggcgggca ttttcggctt gggcgacttg ttcggcggcc tgttgcctgt tgttgccgat
     7381 aaattgccag cttgttgagt ttttcatatt taacgggaaa aattcgcgcg ggttgccgcc
     7441 gggtttgagt atgaagtagt cggtccgacg gttgcggtgc gggttgatgt attcgtaaac
     7501 cgccgcgttg ccttgcggac ggttgcccca ttgcacccac tcgacatgct cgggactgag
     7561 gacggggttg ccggtcagcg tcagtgtcgc ggcttggtca gggttgtgat tgacgatttg
     7621 cgcgccgccg tccgcatggc ggatatgggt aaaggcaagg ttgttgccgt tgaggtcgag
     7681 ccgtccgccc ctgaagccga aatagaggtt ttcgggtttg atttgctggc tgtcggcgag
     7741 gacggccgtg cccctgccgc tggtgatgcc gacttggttg aatgcttgtt tgctgccgtc
     7801 tgaagcagct ttttgggcga gtacgacagt gccttccccg atgctgatgt cgccctggtt
     7861 gatgccttgt ccgttggcga taagcgtgcc tgcgcccagt ttggagagcc ggtcgccttt
     7921 ggggttgctg acttgccaga agacgcgttt gccgtcggct acgataacgc ctgcaccttg
     7981 ccatgtgtgg tttttaccga cgacggtgaa gttgctgtcg aactgcaatg cgcctgcgcc
     8041 ttggttgata ttgtctgcca gcatcagtgt tttgttgtcg aacctgctgg ataggatgag
     8101 cgttttgccg gcatcttcag acggcatgtg cctgctgtcg ttttgtgggg caagcgaagg
     8161 gtttgcaatg ggcagggtga tgcgttcgtt gagcccttgc agggtgctgt cgccattacc
     8221 gttgtcgcgc catatgagtt cgttggtggt cagcccgaca tccagccggg tttcgtattg
     8281 gcggatggtg gaacggatga attcgggttg cgtgacgatg tatttgttga agaagctgtc
     8341 gaagccggcg taggtgctga gtacgcccgc aagcacccag cggttttcat gcttgtcgaa
     8401 ggcgaacagg ggggaaccgc tgtctccggc gactgcgtag gtgttaaggg gttggtcggt
     8461 caggctgtcg ccgacgagta agccgtggtt ttggaaaccc aatactttca gcggcgtgcc
     8521 gccggtcagg tattggtatg ccggggcggt ttgtgtgcgc gtgccgtctg ctttgcggac
     8581 ttgttgcgtg cctgagccga gtcgtacaaa gtatgggaag cggtcggtat cgaggtaggc
     8641 attggccttt ggctggccgt ttccaagcaa gggtacgctg ctgagtgcgg caggtgagat
     8701 ttcggtaacc agtttgttga ggcggggaag gtggtagtcg tagtccgggt gcgggttgcg
     8761 tgatacgagg cggtaggtat aggcttgttc ttcagggttt tgggtgtcgt tgccgaattg
     8821 tatggaaccg tagccgacgt tgtgtttgac actgttgacg tattgggggt gaaccagggt
     8881 ggcgatagcg gtttgccggt tgctgacgcg gaagtcgggc atggggatgc cgctgagaac
     8941 cctgcctaat attttgcctt gcttgtcttg gatggaaata ttgcttgcac ctacggcgaa
     9001 cgcgccttta ttttcggcaa agtcgcgaaa atattggtaa tcgacatcgc tgcggacaat
     9061 ggatgagtaa gcaggggaaa cggcaaaaag accgagggtg gacaatacgg aataaaatgg
     9121 gatggtatgt gtgaagcgca tgatgattat ccgtgtaata gagaaaatca acgattcttg
     9181 cggacaagaa tcaccgatcc cgttttgtgc tggatttata tagtggatta acaaaaacca
     9241 gtacagcgtt gcctcgcctt gccgtactat ctgtattgtc tgcggcttcg tcgccttgtc
     9301 ctgatttaaa ttcaatccac tatattatat attttgtttt tgtttgatta taaggcaacg
     9361 gtgtcctttc gtctattggc gatgattgta tgattttatt taagatttta ataaaaatcc
     9421 taggaatggc tgcgttttcc cgttgtttta tttcgtccgg tattggtttt taagttcacg
     9481 cagcgcgaca aaggtatcga ggctgctgtt taacgttttc ccgctcaatg cctcggcgcg
     9541 gtctctgacg tgcggcaggt cgacgcgcaa aaacggattg acacggcgtt cgtgcgcgag
     9601 ggtaacgggc agggtaggcg tatgcgccgc cgctttcagt gccgtctgaa tgtcggcgtt
     9661 gtccggctcg atatgggcgg caaaatccag gttggcggcg gtgtattcgt gcgccggata
     9721 gaacagggtg ttttcaggca ggcggttgaa gcgttggaag ctgtcgtaaa gctgttcgat
     9781 tgtgccggta aacacgcgtc cgcagccggc ggaaaaaagg gtgtcgccgc aaaagacgtg
     9841 tatgccgtct gaagtttcga gaaggtagct ggtgtggcgg tctgtgtggc cgggtgttgc
     9901 ccaaacggta acctgtccgt cgccgaaggt gaattgggtg cctgcggtta cggtgtgggt
     9961 ggcggcttcg atgtcggatt cgccgtaaac gggcgattcc atgtagccgt gccagagtgc
    10021 cgccgcaccg ccctcgtggt cgggatgggg gtgagttacc catgtttggg caagcatgag
    10081 gcggttgcgg acgaggaatt ccaagacggg cgagggttcg gaagggtcga cgcagacggc
    10141 atggttgccg tgctgtatca tccagatgta gttgtcggtt agggctttga cgggtgtgat
    10201 tttcatgagt gttcctcggt ggacggatac cgtttaaagg atggtatgcc ggaacgccgt
    10261 ctgaaatatt cttcagacgg cattggtttg agtagggcgg ttagcccaag gctttacgtg
    10321 cgccggcaaa gaggcggtac cagccggaca gttccgtcca gccttccggt ttccagctca
    10381 tttgtgcggc gcggtacacg cgttcggggt ggggcatcat gatggtcacg cggccgtcgg
    10441 cgttggtaat gccggcgatg ccttgcggcg agccgttggg gttgagcgga taggtttggg
    10501 taacttggtt ttgaccgtcc acatattgca gcgcgatgcc caaatcggcg gaaatattgc
    10561 cgccgtgaag cgcgaagtcg gcgcggcctt cgccgtggct gacgacgacg ggcaggctgg
    10621 agccttgcat ttcgttcaga atcagcgacg cggatttggg gacatgaacc atgctcaggc
    10681 gcgcttcaaa ctgttcgctc aggttgcgtt tgaacttcgg ccagcctgcc gtgccgggga
    10741 tgatttcggc aaggttgctg accatttggc agccgttgca cacgcccaat gtcagcgtgt
    10801 ccgggtcggc gaagaaggcg gcaaactggt cgcgcagagc agggtggaac aggatggatt
    10861 tcgcccagcc ttcgcccgcg ccgagtacgt cgccgtagct gaagccgccg cacgccgcca
    10921 gcattttgaa gtcggcaagg cggaagcggc ctgccatcag gtcggacata tgcacgtcgt
    10981 aggcatcgaa tccggcacgg gtgaacgcgg cggccatttc gatttgcccg tttacgccct
    11041 gttcgcgcag gatggcgatt ttgggtttcg cgccgctgtt gataaacggc gcggcgatgt
    11101 cttcgttcac gtcgaacttc acgtcggcaa acaatgcgct gcgttcgttg tcgccaatca
    11161 gtgcgaactc gctgtcggcg caggcaggat tgtcgcgcaa gcgttggatt tgatggctgg
    11221 tttcttgcca ggtttgttgc agtttgatta ggttgtcaga aataaggtgc gtttgcccgt
    11281 cgcggatgat taacgtgttc tcatcagtta acgtaccgat ttcaaagaca ttatgatgca
    11341 gctgttgttg atagaataaa ttgataatat cggcaacatc ttgttttcta acttggataa
    11401 cagcacccaa ctcttcatta aataatgtgc gggcaatggt ttcttgccat tcagccaacg
    11461 cttttacctc ttcagtccgc aatgattgag acagagcggt atggttggta ataaatgttt
    11521 gtgcaagcaa taaatttaaa tctatatcca agccgcaccg ccccgcaaac gccatttcta
    11581 ccaaaacggc aaacaaaccg ccgtcgctgc ggtcgtgata cgccaagagt ttgtcttcgg
    11641 cgacaagctg ctgaatcacg ttgtaaaacg ctttcagacg acttgtatcg tccaaatcgg
    11701 gcgcgtcgcc gctcatattg ttgtacacct gaccaaacgc cgagccgccc atacgcgctt
    11761 tgccgaagcc caaatcgata aacagcaata cgctgtcttc gacgtttttc agttcgggcg
    11821 taacggtttt gcgtacgtct tccaccggcg caaacgcgga gataatcagg ctcaacggcg
    11881 aaacgacgga ttttttctct tcgccgtcct gccatacggt tttcatcgac aggctgtctt
    11941 tgcctacggg aatgctcaaa tccaatgcct gacaggcttt ggaaaccgct tcgacggtgc
    12001 ggtagagttt ttcgtcttcg ccttcgttgc cgcacgccgc catccagttg gcggagagtt
    12061 tgatgttgcc gatgtcgccg atattggctg ccacgatgtt ggtgatggct tcgccgacgc
    12121 acattctgcc cgaggcaggc gcgtcaaaca gggcgacggc cggtttttcg cccatagaca
    12181 ttgcttcgcc gcgataggta ttgaagccca tcatggtaac ggcgcagtcg gctacggggg
    12241 tttggtattt gccgaccatt tggtcgcggt gggtcatgcc gccgacgctg cggtcgccga
    12301 tggtaatcag gaagtttttg gcggctacgg caggcaggcg caaaacgcgg taggcggctt
    12361 cggtgatgtc gatatcgtcg gcgtgaaacg gttttttaga tggcgtaact gttttgtcgg
    12421 tacgcgtggt tttgggcggt ttgccgagca agacgttcaa cggcaaatcg atggggttgt
    12481 tggcgaacaa atcgtcgcgc actttcaaat gaccgtcgtc ggtcgctgta ccgaccacgg
    12541 caaacgggca gcgttcgcgt tcgcagatgg cgcggaaggt atccaaatct ttttccaaaa
    12601 tcgacaagac ataacgctct tgcgattcgt tgcaccagat ttgcaaaggc gttaaaccgt
    12661 gttcttccaa cggcacttcg cgcagctcga ataccgcgcc gcgtccggca tcgttgacca
    12721 gttcggggaa ggcgttggac aaacctcctg cgcccacgtc gtggatggag ataatcgggt
    12781 ttttgccgcc gagctgccag cagc
//
LOCUS       DAWHRA010000054        12702 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWHRA010000054 DAWHRA010000000
VERSION     DAWHRA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12702
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.054"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(178..825)
                     /locus_tag="WIB30_001099"
     CDS             complement(178..825)
                     /locus_tag="WIB30_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ3797176.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(822..1190)
                     /locus_tag="WIB30_001100"
     CDS             complement(822..1190)
                     /locus_tag="WIB30_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ3797177.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1200..1871)
                     /locus_tag="WIB30_001101"
     CDS             complement(1200..1871)
                     /locus_tag="WIB30_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ3797178.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIIYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(2036..2755)
                     /locus_tag="WIB30_001102"
     CDS             complement(2036..2755)
                     /locus_tag="WIB30_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ3797179.1"
                     /translation="MATLSDKTILITGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(2839..4152)
                     /locus_tag="WIB30_001103"
     CDS             complement(2839..4152)
                     /locus_tag="WIB30_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ3797180.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLTRHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTIPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            complement(4550..5422)
                     /gene="hpnD"
                     /locus_tag="WIB30_001104"
     CDS             complement(4550..5422)
                     /gene="hpnD"
                     /locus_tag="WIB30_001104"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ3797181.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTILYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDGKTLQYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5593..7455
                     /gene="hscA"
                     /locus_tag="WIB30_001105"
     CDS             5593..7455
                     /gene="hscA"
                     /locus_tag="WIB30_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ3797182.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKIALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVKQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVHAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7532..8188
                     /locus_tag="WIB30_001106"
     CDS             7532..8188
                     /locus_tag="WIB30_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ3797183.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISEVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPRKSK
                     IRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR
                     "
     gene            8224..8985
                     /locus_tag="WIB30_001107"
     CDS             8224..8985
                     /locus_tag="WIB30_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ3797184.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNKPGELTNIEQGESICV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            9040..9381
                     /gene="fdx"
                     /locus_tag="WIB30_001108"
     CDS             9040..9381
                     /gene="fdx"
                     /locus_tag="WIB30_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ3797185.1"
                     /translation="MPKITVLPHTTLCPEGAVIDDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9804..10358)
                     /locus_tag="WIB30_001109"
     CDS             complement(9804..10358)
                     /locus_tag="WIB30_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ3797186.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRDLIALDKAAAGCDSAMLRAQRWLIYTQIAERLAGSGGGFT
                     VTVESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEARVERYPAYCPTVALKV"
     gene            10571..10768
                     /gene="iscX"
                     /locus_tag="WIB30_001110"
     CDS             10571..10768
                     /gene="iscX"
                     /locus_tag="WIB30_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ3797187.1"
                     /translation="MKWTDTQRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10836..11237)
                     /locus_tag="WIB30_001111"
     CDS             complement(10836..11237)
                     /locus_tag="WIB30_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ3797188.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            11449..12408
                     /locus_tag="WIB30_001112"
     CDS             11449..12408
                     /locus_tag="WIB30_001112"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ3797189.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12476..>12702
                     /locus_tag="WIB30_001113"
     CDS             12476..>12702
                     /locus_tag="WIB30_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthetase"
                     /protein_id="HEZ3797190.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNY"
BASE COUNT         2909 a         3368 c         3540 g         2885 t
ORIGIN      
        1 tacaaccacg ccggaattaa gtttaaattt gaataaaagg ttcgggttct gcaaaataca
       61 gaacccgaac cttgttcgga tattgaaacc ggctgcccga ttttgggcgg tgcggcttgc
      121 aagtatcaag attcgcatat gccgtctgaa gctcggagag gttcagacgg catatgctta
      181 tttgggctgc tcttcaacga atctcggacc tttcaagatg ccgttgtgag aatagggcga
      241 cagcaggttg tatgcggcgg ttttggaaac ctgataaccg cggtcggtca ggctgttggc
      301 aatctgattg accactgcgc tgaccaaagc ccccaacagg ccgctgttgc tgttgttgct
      361 gccttcgcgg atgctggccg aacccgacca caactctttt ccgttgcggg aatcgaccag
      421 ccgtgctttg gcggatacgg tcgtcacgct gtctaaaatt tgatatgaag tgccgtattc
      481 ggtaaccgta atgtacaaaa ccgcatcatt gccgaaaatc tgatgcagtt tttccggccg
      541 gacggcgtga atatcggcgg cattggtcaa gccgttttgt ttgaaggttt cctccacgac
      601 tgcggcgggg aagacgtaat agccggcttc ggaaagcggc gcggcggtcg aagccagtac
      661 accccatgtt ccgttgacat cgggcgattc gttcagcggc ggaaccacca aaattgaagc
      721 cggtttgctt tccttgaatg acgtgtagtc gaaatcgggc gctttttgaa cttggcaggc
      781 agacagcgcc aacacggcgg caagccctaa aatcaaaggt ttcatcgctt gcctccttta
      841 cctgttttca tcaggaagtc cataaatacg cccgattcgg gaaacagcct tttctcttct
      901 tcaaactggc ggaacgcgcc ctctttgtct cccgaacggg aaagcagcag tcccagatgg
      961 gcgtgcgcac ccggggcggc attcattttt ttgttgccgg cttccacaaa gtatttttcc
     1021 atcttttcgg tctgcttgcc caacgaagtg tcgtcgtttt tcaaaccttc atagacggta
     1081 tcgggatagc cgccgtaata atacagggat ttttgcccgt tgccgccgca ggcggtcaga
     1141 gccaagaccg ccgcacacag cgacaaacgg ctcaaggttt tcggattcat catttctcct
     1201 taacggttgg gttgccatgc gccgttgtca acagcctgaa ccaggctgtt gacggcttcg
     1261 cggattgcca agtctaaaac tttgccgttc aaagtcgcat cgtagccgga agtgccgccg
     1321 aaaccgatga tttcacggtt ggaaagtgcg tattcgcccg cgccctgtgc ggaatagatg
     1381 atttcggaag tattgacgtt gacgatattc agagccactt ttgcataggc gatttgcgat
     1441 ttgccgcgac ccaaaatgcc gaagagctga tgatcgccga catctctgcg tccgaattcg
     1501 gttacatcgc cggtaacgac ataatctgcg cctttcaggt tatgcgcttt gccggaaatg
     1561 ccggattcct gttttaatgc gttcaaattg gtgcggttca gtacgttgaa gcggttggtc
     1621 tgttgcaggt gcgttaccag aatggttttt gcctggctgc ccaaacggtc ttccccgtcg
     1681 gagaaaatgc ctttttggaa gctggagcgg ttgtcgaatg ttccgacgga aatcggggta
     1741 cgaacaccgt gatattgcgt attgtaggag gcgactttct ctacctcgag actgcgtgag
     1801 gattcggtcg cacagccggt cagtgaaacg gcagcggcgg caaggacaac ggcggtggaa
     1861 acggttttca taaaatttac cctaaggtca agttaaggaa ataacgggtt atcattattg
     1921 tccttatgta aatttaagtc aaggtgtttg tctgtgcggg acggatgcgc gcggaaggta
     1981 cggatatttt tcaaacggaa tcgcggcgcg ggcgggatgc ctgccttttc cggatttaga
     2041 ggtaaacgat ttcgccactc cgccctttgc tttcggcact tgcccaccag acaaatgcgg
     2101 gcagcacgtc cccgtagctt ttgcgttcgc ttttggcttc gcccggatgg gatttgatgc
     2161 gttgcgggga attgatgggg ccggggacga ggacgttggc gcgcaggttg ccgaagcgtt
     2221 cccattcgtc ggcggcgact ttgcacaggt agttcaacgc ggctttggac gcgccgaagc
     2281 cgccccagta ggctttgggt gtttcgccgt ggctttcgcc gacgaagatg acggacgcgt
     2341 cgggcgactg cttcagcagc gggaacaggg cgcgggtcag ccccataggt gcgacggtgt
     2401 tgatgcggta ttggttgacc cattcggcga cggtttggaa atccagcggc gagagggcgt
     2461 aaaaatagcc ggcgcagtgg acgatgccgt ccagtttgcc ttgcgtggct tcggcaatgg
     2521 tggcggcgaa ctgattgaat tctttttctt ccgcactcat caggtcgaaa cggatggcga
     2581 agggttcggg gtgtccggct tcgacaatcg cgtcatagac tttttccagt tttttctgat
     2641 ggcgtgccac cagaatcacg gttgcgcctg ccgccgcata ggctttggcg acctgttcgc
     2701 ccagaccttg cgatgcgccg gtaattaaga tggttttgtc ggacagtgtc gccatacttt
     2761 ttcctttttg gtggtcggtc gggggtattt tagcgttttg ccgcaccttg taaagcgtcc
     2821 cgatgccggc cggcgttttc agacggcatc gctcaggctt tgcaggcagg tttccgccgc
     2881 cgcgaaacct gattgtacgg cggcttcgag cgtggcgggg tagtccgggt ggaggtagtc
     2941 gccggcgggg aagatgcggt gccggtgcag ccacgacaaa tccggcggcg gggcatcggc
     3001 tgcggttgtg gcgcgttttt cggtgatgac gcgcacggct tcgggttcgc ccaaatgcgg
     3061 aaggatgcgt ttgaggtcgg cgtgaacttt gtccgcccac gctcggtttg taaacgcgcc
     3121 gacgcggtcg gaaacgctga tgacggcgga cacttcgttt tcaggcagtc cgagcctgcc
     3181 ccggcaaagc agccattgtg ccgtgccgtc ggcaaggccg gtcagcgggg cgggcaggcg
     3241 gacgggttcg gcgtagcgca gatagacggt ggtgatggcg tgatagcgaa ggttttgata
     3301 tgccgtctga acgtgttcgg gcgtgccttc gggcaggagc gcggcggcgt ggtagggcgc
     3361 ggtggcgggg acggcggcat cgaaagcttc gccgttgacg agcactttcc cgtccgggat
     3421 ggtgttcaga cggcatacgc gcgtttcgag gcggatgtcc gcgccgagcc gttgaagctc
     3481 cgccaaggcg ggttcggcga cgattgcgcc caaatcctgc ttgggtagga gatagtcgct
     3541 gccggatttt ttcgtcagca cgccgtcgga caaaacgttg cacaacacgc gcaggcttgc
     3601 ggtttccaaa ggcgtgttga gcgcgcccca caccagcggc tgccaaaact gcatcacggc
     3661 ggcacgcggc acgttccgct gtttcagcca ttgtgcaact gtcgtgtcgg gctgtccgag
     3721 gcgtgcggac ttctgcaaat cggacatatc ggcaagcagt ttggctttga atgcggacgg
     3781 tacgcgccgg gcaagcagca cgccgcccaa aatatgcagc ggtgcgggca gggggagggc
     3841 gcggaactgc aaaccgccgt gcatatgcca gtgcagcggt acgcgcaaaa aggcggcacg
     3901 gggatccgaa ccgatggttt tcatcaggcg caacacgccc cggtatgcgc cgagcaaaat
     3961 gtgctgcccg ttgtccaaaa aaccgaaacc gtcggtattt ccggccagtg tgcgcgccct
     4021 gccgcccgcc tgccggccgg cttcaaacag ggtaacgtcg gcgtgccgcg tcaaggtgac
     4081 ggcggcggac aagccggccc agcctgcgcc gatgacggcg attttcgggc gcggatgcgg
     4141 cgtgttcatc atttattcct ccaatggttt tgcagccgta tctatttccg tttccgaaaa
     4201 tgacggtaga aaaggataca ggctaaaaat aaaggcagca gaaagcgcgc atagggatac
     4261 cacggatggt ctgcatgcca gtatccgtac cgtccctgtg gaacggtatc atagcggtaa
     4321 ttgtcgggga aaaagttgat ccaaaccgtt actgccagcc ataaggctat atccctgact
     4381 gctttcattt acttctgctc ctgtttaaat tcccagcaat tcgatttcaa agcgcgaacg
     4441 ccaacgggat tgcgcggtta cgatgcagac tttcagacga tggttgaaac cccgtccggc
     4501 ggggctgtgg gaatcgggtt tgcctgacgg gcggcgggcg gtatgcgcct tatgcccgtt
     4561 ccggcgtgcc ggggcgcggt ttgaatccga ataaccaggt tttcagggca atgcgttttt
     4621 tgcgcggcga agggagggcg attttgtatt tgaggacgtt ttgcgcgccg tcccggtcga
     4681 tttcgttcaa tagctcgtaa taaaccgccg ccataaccag tccgactttt tgggcttttt
     4741 tatcggcagc cggcagcagc gatacggctt cgcggtaggt ttcacgggcg cgtttgattt
     4801 ggaacgccat caattcggca aaattgcccg tcgggctgca ttgcaaaatc acgcttgcgg
     4861 ggacatcaaa ccgctgcatt tcctccatcg gcaggtaaat ccgtcccctg cgcgcgtctt
     4921 cgccgacatc gcggatgatg ttggtcagtt gcagcgcaag tcccatcttg tcggcgtatt
     4981 gcagcgtttt gccgtctgaa aaccccaaaa tccgcgcaat caggcagccg actacgcctg
     5041 cgacgcggtg gcaatacagt ttcaattcct caaaactgcc gtaacgcgcc tgaaccaaat
     5101 ccatctgcat cccgtcgatt aaggcttcca gttcatattt cggcagcttg aaggtttcct
     5161 taacctgccg caaggcctga ttgacggggt gttccggcat cgcgccgccg aataccttgt
     5221 ccaaatcgcc gcgccaccag ttcaatgttg cctgtgcaac atcggggttg gaacattcgt
     5281 caaccacatc gtccaattcg cggcaaaagg catataaaat cgttaccgca tcccgttttt
     5341 cctgagtcag gaaacggaag cccgataaaa aactggagcg gctttcttct gctttttgac
     5401 ggcaatagtc gagtcctttc acgatttata ttcctaatga tgggcgggaa aggcggattt
     5461 tatcggcatt tggcggtaga gggcaatttc ggcggcacga cccaatcctt agcggtttgc
     5521 tcaactatcg gcgcaaattc tgttaaaatg ccgctttcct tcctttacac accgcaccga
     5581 caggcagaat ttatggctct tttgcagatt tcagaaccgg gtatgtccgc cgccccgcac
     5641 cggcaccgtt tggcggcagg catcgatttg ggtacgacca acagcttggt cgccaccgtc
     5701 cgcagcggca gtgccgcctg cctgcccgat gccgaagggc gcgttaccct gccttccgtc
     5761 gtccgctatc tggaaaacgg cggcattgaa gtcggcaaaa tcgccctgtc cgcccaaaaa
     5821 accgacccgc tgaacaccgt cagctccgcc aaacgcctta tcggacggac gcttgccgat
     5881 ctgcatcaaa atacgcatta cctgccttac cgtttcggcg acaatcaacg cgttatcgaa
     5941 ctgcatacgc ggcagggggt gaaaacgcct gtcgaagtgt cggcggaaat cctcaaaacc
     6001 cttaaatcgc gcgccgaaga aaccttgggc ggcgatttgg tcggcgtggt gattaccgtc
     6061 cccgcctatt tcgacgatgc ccaacgccag gccaccaaag atgccgcgcg tctggcgggt
     6121 ttgaacgtat tgcgcctgct caacgaaccc accgccgccg caatcgccta cgggctggac
     6181 aacgcctcgg aaggcacgtt tgtcgtgtac gacttagggg gcggcacatt cgacgtatcc
     6241 gtattgcaac tgaccaaagg actgtttgaa gtcaaagcca ccggcggcaa cagcgcgttg
     6301 ggcggcgatg acttcgacca tcgtttgttc tgctacctgc tcgaacaaaa cggactctcc
     6361 caactcaacg aacaagacag ccaactcctg ctttcgctcg tccgcgccgc caaagaacaa
     6421 ttaaccacgc aaaccgaagc gcgcattcag gcgacgcttt cagacggcat ggcaatcgac
     6481 acaagcatca gtcgcgccga gttccacaac ctgacgcagc atttggtgat gaaaacgctc
     6541 gaaccggtca aacaggcgtt gaaagatgcc ggtgtcggta aaaacgaagt caaaggcgta
     6601 attatggtcg gcggttcgac ccgtatgctg cacgtccagc aggcagtcgc cacctttttc
     6661 gggcaaaccc cgctgaacaa cctcaacccc gacgaagtcg tcgcgctcgg cgcagccata
     6721 caggcgaacg tcctcgcagg caacaaagcc gacggcgaat ggctgctgct ggacgttacg
     6781 cccttgtcgc tcggtttgga aacctatggc ggcttggcgg aaaaaatcat cccgcgcaat
     6841 tccaccatcc ccaccgcgcg cgcgcaggac tttaccacct tcaaagacgg tcagaccgcg
     6901 atgacgatac acgtcgtaca aggcgaacgc gaactggttg ccgactgccg cagccttgcc
     6961 aaattcaccc tgcgcggcat tccgccgatg gcggcgggtg cggcgcgtat ccgcgtaacc
     7021 ttccaaatcg atgcggacgg gctgctgtcc gtttccgccc aagaacaaag taccggcgta
     7081 cacgcgcaaa tcgaagtcaa accttcctac ggtttggacg acggcgcgat tacccgaatg
     7141 cttaaagaca gtatggacaa cgccgccgaa gatatggcgg cacgcgcccg cgccgaagcc
     7201 gtggtcgaag ccgaaagcct gaccgatgcc gtcaacgccg ccctcgagtt ggacagcgat
     7261 ttgctggatg ccgaagaatt gcaacagatt cggcaaggta tcgccgattt gcaaggccgt
     7321 ctgaaagacg gaaaagccga agacatccgc gccgccgccg ccaaactcgg cagcatcacc
     7381 gacaatttcg ccgccaaacg catgaaccgc aacatccaac gtgcgctgac aggccagagt
     7441 gtcgataata tttgatactt aaacggtttc agacggcata gagataacct gatgccgtct
     7501 gaaggctcga aaacacttga aaaacatcga tatggaaaag tcaggcattg tctatctgat
     7561 gaaaaccgtc atcaagggcg tatataaaat cggcatttcg gaggtaagca attttgaagg
     7621 cagaatgcgc catttggaaa acaacggcta tgcgaacgtt gccgggttgg aacgcatcct
     7681 cgctgtcaaa accgacaatt acaaagaaaa agaaaacctg ctccatgaaa ttttcagcaa
     7741 aagcaggata ggcgataccg aattgttcgc cgtggacgaa aaccttgtga aacgtttgtt
     7801 tttatcgctt cgcggcgaaa tcgtgttccc gaaaaacgaa acggcggaat cggaatttga
     7861 aaaaagcgtc cacgaacgca ggcaggaagg gaatgccggg tcaggccgca aacaactgct
     7921 tgatttggta cggcgcggac accgggaata cccttacgcg ctgccccggc ttttggcggg
     7981 cgcggcatcc tacaagccga gaaaatcgaa aatccgcctt tttaaagaag catatttcgg
     8041 caaaagcggc acgaggctga ccgacgaaat tgcagacggc atccatattt acacctgttt
     8101 ttcgcgggcg gatttggaaa aagcctattc cgaatatttg gaacttttca aatccgaatc
     8161 ggatgccgaa ggcagaaaac cacggtaagg tgcaaacaga taccgtacac gttgaggagc
     8221 agtatgatgg gcgattccgt catttattat gtagaacagg cagacgaacc ggtaaaccgt
     8281 gccggcgaac gcgcccgtaa aacattcaaa tatttttggc gcgagctttt ttgggaacgc
     8341 cgccgcatca ttcccgcctt agattttgcc atggtcaaag cccctttttt ccaagacggc
     8401 gaagacggcg aaatttgcga acatatgtgg atcgacgata tttattttga cggtctttat
     8461 atttacggtg tgctgaacaa taaacccggc gaactgacca atatcgaaca aggcgaaagc
     8521 atttgcgttc cggttgacga catcagcgac tggatgttcg tgtgcaacgg cattccctac
     8581 ggcggcttta ccatacaggc aatgcgtgga cagatgacgg aagaggagcg taccgaacac
     8641 gatgccgcat ggggaatcga tttcggcgat cccgggcagg tattgctggt gtatgaagaa
     8701 aaagaacatc ccgaaaatct ggaagagcat ccgatgtgcc ggaactgtat tgacgatttt
     8761 cggcaacagt tgtcccaaaa tccggatttt ctgcatgagc aggacgaaga cggctatacg
     8821 ccgcttcatc atgaagccat cgcaggaaat gcacttatgg ttcaagccat gcttgaatac
     8881 ggcgcaaatc ctgcctcaaa gacatcggaa ggctataccg ccctcgattt tgcccgcctg
     8941 acgggctggc aaaatgttgc cgacctgctc gaaccgcgac attaggcaga cagttttccg
     9001 aaaacgaaca caaacacttt ttacagaaag acaataaaaa tgcccaaaat caccgtactt
     9061 ccacacacga cattatgccc cgaaggcgcg gttatcgacg acgcgccgga aggcaaaacc
     9121 gtcctcgacg tactgctcga ccacgacatc gaagtcgatc acgcctgcga aaaatcctgc
     9181 gcctgcacca cctgccacgt catcatccgc aaaggtttcg acagtttgga agaaccgacc
     9241 gaattggaag aagacctgct cgaccaagca tggggtttgg aagccgattc gcgcctgagt
     9301 tgtcaggcgg ttgtcgccgg cgaggatttg attgtggaaa tccccaaata caccatcaac
     9361 cacgcgcgag aagaacactg aaaacaggcc gtctgaagcc ggcgcgcttc agacggcatt
     9421 gttgcgcgga taaggcgcaa tcgcccgaaa acaggcgttc gtacaggcgg aaatttcgat
     9481 tctatagtga attaacaaaa atcaggacaa ggcggcgagc cgcagacagt acagatagta
     9541 cggcaaggcg aggtaacgct gtaccggttt aaatttaatt cactatatat tgatttttat
     9601 cggttttctg acggaataat ccagtgcggc atccgaggcg gattactcgg acgcgatgca
     9661 ccggtattta tcggttttgc agccggaaaa accgccggcg ggttatagtg gattaaattt
     9721 aaaccagtac agcgttgcct cgccttgccg tactatttgt actgtctgcg gctcgccgcc
     9781 ttgtcctgat ttttgttaat ccactatact tttagggcga cggtcgggca gtatgccgga
     9841 tagcgttcca ctctcgcttc tatattgatt ttgattggtt ttctgacgga atgacccgat
     9901 gcggcatccg ggacggcttg tgttttttcc tgcccgcctg ccggattttc ccatccttgc
     9961 gtgaaaccga aagagacggc ggcgcggcgg acaagctcga gatagcgtcc ttcaagctcc
    10021 ggacaggcgg cggacacgct ttctaccgta accgtgaaac cgccgcccga cccggcaagg
    10081 cgttccgcaa tttgcgtgta gatgagccaa cgttgggcgc gcaacattgc cgaatcgcaa
    10141 ccggcagccg ctttatccaa ggcaatcagg tcgcgccggt tttggtggtg aaggcaaacc
    10201 gttactctgg agaggatatg ttgcccgtcc aatatttgat acagtttgcg tatcagcaga
    10261 atcaggcggt caaactcctc catctccgac agggaatcag gatggtcgga agcggtataa
    10321 accagtttgg acaattttgc ggcaaacata ttgttcatca atcttccttg tcggttgaaa
    10381 ccccgctctt cggggcggta gaatcagatt tgtttgggag ggacaactcc tcccagatca
    10441 ggacgacaca taggctggtg cttgatgtgt tgtccggcga gttgaaacat tcagcaatcc
    10501 tcaaggggcg gcagttttgc cgaaacatat tctacacggc ttcaatgccg gacgataaaa
    10561 ggaaattcat atgaaatgga ccgacaccca gcgcatcgcc gaagaactct atgacctgca
    10621 cggcgaaacc atcgatccca gaaccgtgcg ctttacccaa ctgcgcgacc tgattatggc
    10681 attgcccgaa tttgacgacg accccgcccg ttgcggcgaa cgcatcctcg aagccgtgca
    10741 gcaggcatgg atagacgagg cggaataagt ttcgggaatg ccgtctgaaa tgcggcggta
    10801 cgcggttcgt gcttctgttt gcagcgggaa tggttttacc agtctccttt tttcagcctg
    10861 tccagttggc ggcggtcgcg cttggtcggt ctgccgtcgg gataggcgga agtgatgcgg
    10921 ctgaattggt cgagctgttt gcgctcttcc ctcaatgttg ccgttttcgc gtcctcttca
    10981 tacagaagcc gcgcctcgga tgccgggcgg cgttggtggt tcaaaccttt aaccttgatt
    11041 ttatagggaa gggaattgag cgtcaggtcg ataatatcgc cgatgtctat ggttttactg
    11101 tttttgacct tcgagccgtt tacttgaacc ctacccagtt cgatgtgctt ttgcgcaagg
    11161 gaacgggtct tgaaaaaacg tgccgcccaa agccatttgt ccagccgcat ggcggaagaa
    11221 tcgtgcttgt ctttcatacg attttgtttg aaataattga atttgtttcg agtttagcat
    11281 aagatacgcc gccttataac tagtatatat gcactaatcc actgttttcc atgctgtccg
    11341 aacacaaaaa gagggtatgg aaaagccgtt ttggacaata aattaactgc ggaatatgca
    11401 caaatagcgt atgatagcgg cagaatctgt tgatgagagc ttcattctat gaaacctgtt
    11461 tttttggatt ttgaacaacc catagccgaa ctgaccaaca aaatcgatga gctgcgtttc
    11521 gtccaagacg agtctgccgt cgatatttcg gacgaaatac accgtttgca gaaaaaaagc
    11581 aacgacctga ccaaatcgat tttcagcaaa ctcacacccg cccaaatttc acaggtttcc
    11641 cggcatccgc agcgtcccta tactttggat tacattgagg cactgtttac cgattttgaa
    11701 gaactgcacg gcgaccgcca ctttgccgac gattatgcga ttgtcggcgg attggcgcgt
    11761 ttcaacggac aaagcgtgat ggtcgtcggg catcagaaag ggcgcgacac caaagaaaaa
    11821 atccgccgca acttcggtat gccccgtcct gaaggctacc gcaaagccct gcgcctgatg
    11881 aagacggcag aaaaattcgg cttgcccgta atgaccttta tcgatacgcc gggcgcgtat
    11941 cccggcatcg gcgcggaaga acgcgggcag tcggaagcca tcggcaaaaa cctgtacgaa
    12001 ctgacgcgcc tgcgcgttcc tgttttgtgt accgtcatcg gcgaaggcgg ttcaggcggt
    12061 gcgttggcgg tcgccgtagg cgattacgtc aatatgctgc aatactcgac ctattctgtt
    12121 atctcccccg aaggctgcgc gtctattttg tggaaaaccg ccgaaaaggc ggcggatgcg
    12181 gctcaggctt tgggcattac tgctgaccgc ctgcaaaagc tggacttggt cgataccgtc
    12241 atcaaagaac cattgggcgg cgcgcatcgg gatttcgggc aaaccatgaa aaacgtaaaa
    12301 gccgttttgg aaaaacaact gcacgaagcg caaagcatcc cgcttgccga tttgctttcg
    12361 cgccgtttcg accgcattat ggcttacggc aaattttcgg aacaataatt caggtagaac
    12421 aagcagcaag cagtttgtct gaaactgctt gctttttctt tatcgggacg gaaccgtgct
    12481 gactttagat gcgtttgagc aatgcttgaa ggattgtttt cctcaaggtc tgaatggaaa
    12541 aaaaacagcg gtggcattaa gcggcggctt ggattccgtc gttttgctgc atctgcttgt
    12601 ccgcgccgga aaaaagggcg gttttattcc ggatgcattg catatccatc acggcttgag
    12661 tccccgtgcc gacgattggg cagatttctg ccaaaactat tg
//
LOCUS       DAWHRA010000055        12224 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWHRA010000055 DAWHRA010000000
VERSION     DAWHRA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12224
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.055"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            90..1481
                     /gene="hrpA"
                     /locus_tag="WIB30_001114"
     CDS             90..1481
                     /gene="hrpA"
                     /locus_tag="WIB30_001114"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ3797191.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKAIAKNQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMAALKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            1625..3268
                     /locus_tag="WIB30_001115"
     CDS             1625..3268
                     /locus_tag="WIB30_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ3797192.1"
                     /translation="MDEIQIPKKVELQTKLENEKIVLSKGSTTIIVGANGTGKTRLAV
                     YIEEQLKEKAHRISAHRALKLNPNVNKIPEKSAKTYLSYGQNWDGIDVSNRKNYRWDN
                     NSYTHLLNDFDWLLQYLFAQQNNIAVANNQKLNRNEKVTNSKTKLDILQEAWETLLPH
                     RKLHITADDIQVSAVDNEELYSASNMSDGERALFYILGQVLSVDDGSVLIFDEPELHI
                     HKSIISNLWDKIEELRPDCSFLIITHDIEFAATRVAKKYVIRNYYPTPAWDISEVPES
                     NFDEETITMILGSRKPILFVEGNNNSLDIATYRYCYPDWTIIPKGACKDVIQSVSSLK
                     KLSNEMPLLNLKCSGIVDLDSRDEREIEQLNNLGIYILPVSEIENLFSLTDVAKEILK
                     LNQYSDEELLNKLNGFKSELIKYIDNELKDDKLDEFVVKQVRRKIDNYLKNIDLSSKI
                     TSTDMKKSLLNEISTLTEQKIETWISEIKNEIQRCIEQQDLDKLLTIYDNKGLLAKSA
                     CVLKGMRNKHEFESWIMRTLKGRNKDFIDAIRQKLPILD"
     gene            3312..3494
                     /locus_tag="WIB30_001116"
     CDS             3312..3494
                     /locus_tag="WIB30_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797193.1"
                     /translation="MKNHQTTLSFPTSSSLKPCADKQGLSEDRSQYRLTKLGEQMAHL
                     PIDPKIARILLVLFRF"
     gene            3706..5283
                     /gene="lpt3"
                     /locus_tag="WIB30_001117"
     CDS             3706..5283
                     /gene="lpt3"
                     /locus_tag="WIB30_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ3797194.1"
                     /translation="MMKKSFLTLVLYSSLLTASEIAYRFVFGIETLPAAKIAETFALT
                     FVIAALYLFARYKVTRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPVLWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSRIPAFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNAIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENEMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGKHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            5385..8756
                     /locus_tag="WIB30_001118"
     CDS             5385..8756
                     /locus_tag="WIB30_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ3797195.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQIARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDASAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEVRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYAAQQTTPSPVGEGRGEGKTVAAQTNFSATSTNPLPQERE
                     QSAAASTISDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATSTNPLPQEREQSAS
                     ASTFSDDLRPANLQQPSPSPVGEGWGEGKTVATQTNFSATSTLSDDSKPKKQPAPQKN
                     RLKPLPLADIRTFQAWLKTAERDNLRLLFLSRDDLMQHAAAHITEEQFPKFWQTADGK
                     FKLSYRFEPHHPLDGVTMTVPLTVLNRLHAPSLEWLVPGMIREKIQLQIKALPKQIRR
                     ICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRIFEQINQDEWAAFRLPEHC
                     YFNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQEFERDNVTAWDIGTLPES
                     IKFARGKQQLTGYLGLQKEKDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQVKDL
                     NKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDELPRNEKAFKEQIKRARSR
                     LPAVKEALSRYLQETAAVYAELNSKLGKHPLTHLLRLRLQTLLAAGFATRTPWAQWPR
                     LPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDSLIKQGLPISDGLAAFK
                     WMIEELRVSLFAQELKTPYPVSVKRLLKEWEKIEK"
     gene            complement(8789..9880)
                     /locus_tag="WIB30_001119"
     CDS             complement(8789..9880)
                     /locus_tag="WIB30_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEZ3797196.1"
                     /translation="MQTFNRERLEFALDKRGCTQTKLAELLGITARQVRNYVKDEQIP
                     DLNELANLLEFPVHFFTNDERLPELKSQAVSFRARSRTSKRLEKQALNHSITAFLLND
                     WLETEFTLSQAVLPDFSDISPEEAAQELRLEWGLGNEPIPNMITLLEAKGVRVFSLSL
                     DTKDIDAFCTWYENHPFVFLNTQKSAERSRFDAAHELGHLIRDKYSMEHSKNSEITED
                     EPRDIIEKEANAFASAFLMPEAALRLYRHVPITIENLLKIKRRFGVSLVALAYRMHKL
                     GMITDRMYIHNLCPLFAKKKYRTIEPEPMEREISSALRKMLTILKNDGIGIEQIAENL
                     NVSPKDVADLTFGLVERDLNRYRKLRLVK"
     gene            complement(9896..10540)
                     /locus_tag="WIB30_001120"
     CDS             complement(9896..10540)
                     /locus_tag="WIB30_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797197.1"
                     /translation="MVEFALSEPFPFEVGEIVEQQNSDIALSEISKYLSQEMLKEIVL
                     TAHNNIQDKAALSDRNTLGMYRQAEITFALRVYLSKDGWTLEPWSGGAFLCLSPCRTK
                     SIIVATATSDVGRHHGMPMTTARKGKGFEEAIRNSKLDLPDTVQFWVLLFHWKYNKVQ
                     LELSLPSGFVSHQITGYKQRIILADVDLTKFDELERKQPVEEYEPLVERKQKAG"
     gene            10897..11049
                     /locus_tag="WIB30_001121"
     CDS             10897..11049
                     /locus_tag="WIB30_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797198.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWV"
     gene            11419..11574
                     /locus_tag="WIB30_001122"
     CDS             11419..11574
                     /locus_tag="WIB30_001122"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ3797199.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(11664..12113)
                     /locus_tag="WIB30_001123"
     CDS             complement(11664..12113)
                     /locus_tag="WIB30_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ3797200.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         3634 a         3062 c         2525 g         3003 t
ORIGIN      
        1 tttcagacgg catttcccca tcccgatatt gcctaaacaa aaccgaagcg tttgctataa
       61 ttctattttt taccgcatac gcaccaatca tgtttcccga tttctcccaa accctctcca
      121 aagaccgcca cttcctgcgt tccgccttca aaaatcccaa caaatacggc ggtttgtcca
      181 aaatcgaaga aaaataccga aaatcgcacg aaatcttttt gaagcgtttg gcagccttgc
      241 caaaacccga attcgacaac accctgcccg ttcacgagaa gctcgaagaa atcaaaaaag
      301 ccattgccaa gaatcaggta acgattattt gcggcgaaac cggttcgggc aaaaccacgc
      361 agttgcccaa gatttgcttg gaactcgggc gtggggcggc aggattgatc gggcataccc
      421 agccgcgccg tttggccgcg cgctccgtag cagagcggat tgccgaagag ctgaaatccg
      481 aaatcggcag cgcggtcggc tataaagtac gcttcaccga ccacacctcg cgcgatgcct
      541 gcgtcaagct gatgaccgac ggcatcctgc tggcggaaac gcagaccgac cgttatctcg
      601 ccgcctacga cacgattatc atcgacgaag cgcacgagcg cagcctgaac atcgacttcc
      661 ttttgggcta tttgaaacaa ctcctgccgc gccgccccga tttgaaagtc atcatcacct
      721 cggcaacgat agacgcagaa cgcttctccc gacacttcaa cggcgcgccc gttttagaag
      781 tgagcggacg gacgtatccc gtcgaaatcc tctaccgacc gctgaccggc aaagacgaag
      841 acgacgcaga agtggagttg accgacgcga ttgtcgatgc ggcggacgaa ttagcgcgac
      901 acggcgaagg cgatattttg gtattcctgc cgggcgagcg cgaaatccgc gaagccgccg
      961 aagccctgcg caaatccacc ctgcgccgca acgacgaaat cctgcccctg ttcgcacgcc
     1021 tgtcgcacgc cgaacagcac aaaatcttcc acccctcagg cgcgaaacgc cgcatcgtat
     1081 tggcaaccaa cgtcgccgaa acctcgctta ccgtgccggg catcaaatac gtcatcgaca
     1141 ccggcctcgc gcgcgtcaaa cgctattccg cacgggcgaa agtggagcag cttcatatcg
     1201 aaaaaatctc ccaagccgcc gcccgccaac gatccggccg ctgcggacgc gtctccgcag
     1261 gcgtgtgtat ccgactgttt tcagaagaag attttaacag ccgccccgaa tttaccgacc
     1321 ccgaaatcgt ccgcagcaac ctcgccgccg tcatcctgcg catggcagca ttgaaactcg
     1381 gcgatgtggc ggcattcccg tttttagaaa tgcccgattc acggtatatc aatgacggtt
     1441 ttcaggtgtt gttggagttg ggggcggtgg aggccgtctg aaaacaggca gacataaaag
     1501 aaaatccgcg tagagtgatg taaacttacc cttgctttaa taagtagaaa atggtgggtt
     1561 tacgtccccc cctgcggcta ctaaaaaaat ataagagtaa acaacctttt tgaaagaaaa
     1621 atgtatggac gaaattcaaa tacccaaaaa agtggaatta caaaccaaac tagaaaatga
     1681 aaagattgtt ttatcgaaag gttctaccac gattattgtt ggtgctaatg gcacagggaa
     1741 aacaagatta gctgtttata ttgaagaaca attaaaggaa aaagcacaca gaatttcggc
     1801 tcatagagca ttaaaattaa accctaatgt caataaaata ccagaaaaga gtgccaaaac
     1861 atatctatct tatggtcaga actgggatgg aatcgatgta tcaaatagaa aaaattatag
     1921 atgggataat aactcatata ctcatttact caacgatttt gattggttat tacaatattt
     1981 attcgctcaa caaaataata ttgcggtagc aaataatcaa aagctcaacc gtaatgaaaa
     2041 agtaaccaat tcaaaaacaa agctagatat tttgcaagaa gcatgggaaa cattattacc
     2101 acacagaaaa ttacatatta cagcagatga tattcaagtc tctgctgtag ataatgagga
     2161 attgtattct gcctcaaata tgagtgatgg agagcgagca cttttctata ttcttggaca
     2221 agttttgtca gtagatgacg gttctgtctt aatttttgat gagcctgaat tacatattca
     2281 taaatcaatt atttcaaatc tatgggataa aattgaagaa ttacgacctg attgttcatt
     2341 tctaatcatt acacacgata ttgaatttgc tgcaactcga gtagctaaaa aatatgttat
     2401 cagaaattat tatccgaccc ctgcttggga tatttctgaa gttcctgaaa gtaattttga
     2461 tgaagaaaca ataacgatga ttttaggtag ccgtaagcca atattatttg ttgagggcaa
     2521 caataatagt ttagatattg ctacttaccg ctattgttat cctgattgga ccatcatacc
     2581 caaaggggca tgcaaagatg tcattcaatc agtatcatcg ctgaaaaaat taagtaatga
     2641 aatgccatta ctaaacttaa aatgttcagg tattgtcgat ttagatagta gggatgaaag
     2701 agaaattgaa caattaaata atttgggtat ttacatttta cctgtatccg aaattgaaaa
     2761 tctttttagc ttaactgatg tagcaaaaga gatattgaaa ctaaatcaat attcagatga
     2821 agaattactc aataaactta atggatttaa atccgaacta attaaatata tagataatga
     2881 attaaaagac gataaattag acgaatttgt tgtaaaacag gttcgacgta aaattgataa
     2941 ttatttaaaa aatattgatt tatcctccaa aataacaagt actgatatga aaaaatcatt
     3001 acttaatgaa atttctactt taacagaaca gaaaattgaa acatggattt cagaaattaa
     3061 aaatgaaatt caaagatgta ttgaacagca agatttggat aaattactta ctatatatga
     3121 taataaagga ctcttggcta aatcagcttg tgttttaaaa ggaatgcgta acaaacatga
     3181 atttgaaagc tggataatga gaacattaaa aggaaggaat aaagatttta ttgatgcaat
     3241 cagacagaaa cttccaattc tggattaaat aaaaccatct gaaaatttac cttcagatac
     3301 agatatattt catgaaaaat catcaaacta cactctcttt ccctacttcg agtagcctga
     3361 aaccttgcgc agacaaacaa ggcctgtctg aagaccgcag ccaataccgc ctgaccaaac
     3421 tcggcgaaca aatggcgcac ctgcctatcg acccgaaaat tgcgcgtatt ttgttagtat
     3481 tattccgttt ttaaaaatgc ccgattcgcg gtatatcaat gacggttttc aggtattgct
     3541 ggaattgggg gcggtggagg ccgtctgaaa ataaaatctt tctttataaa aaggcaggcc
     3601 atgtttcatt ttcagacggc ctaaatcatt gagaaactaa aaactattaa aaagggaata
     3661 ttgggtttta aaactcaatc ggtaaatttt tattgtgaaa tattaatgat gaaaaaatct
     3721 ttccttacgc ttgttctgta ttcgtcttta cttaccgcca gcgaaattgc ctatcgcttt
     3781 gtatttggga ttgaaacctt accggcggca aaaattgcgg aaacgtttgc gctgacattt
     3841 gtgattgctg cgctgtatct gtttgcgcgt tataaggtga cgcgtttgtt gattgcggtg
     3901 ttttttgcgt tcagcattat tgccaacaat gtgcattacg cggtttatca aagctggatg
     3961 acgggcatca attattggct gatgctgaaa gaggttaccg aagtcggcag cgcgggtgcg
     4021 tcgatgttgg ataagttgtg gctgcctgtg ttgtggggcg tgttggaagt catgttgttt
     4081 tgcagccttg ccaagttccg ccgtaagacg catttttctg ccgatatact gtttgccttc
     4141 ctaatgctga tgattttcgt gcgttcgttc gacacgaaac aagagcacgg tatttcgccc
     4201 aaaccgacat acagccgcat caaagccaat tatttcagct tcggttattt tgtcggacgc
     4261 gtgttgccgt atcagttgtt tgatttaagc aggattcccg cctttaagca gcctgctcca
     4321 agcaaaatcg ggcagggcag tgttcaaaat atcgtcctga ttatgggcga aagcgaaagc
     4381 gcggcgcatt tgaagctgtt tggctacgga cgcgaaactt cgccgttttt aacccggctg
     4441 tcgcaagccg attttaagcc gattgtgaaa caaagttatt ccgcaggctt tatgactgca
     4501 gtgtccctgc ccagtttttt caatgcgata ccgcacgcca acggcttgga acaaatcagc
     4561 ggcggcgata ccaatatgtt ccgcctcgcc aaagagcagg gctatgaaac gtatttttac
     4621 agcgcgcagg cggaaaacga gatggcgatt ttgaacttaa tcggtaagaa atggatagac
     4681 catctgattc agccgacgca acttggctac ggcaacggcg acaatatgcc cgatgagaag
     4741 ctgctgccgt tgttcgacaa aatcaatttg cagcagggca agcattttat cgtgttgcac
     4801 caacgcggtt cgcacgcccc atacggcgca ttgttgcagc ctcaagataa agtattcggc
     4861 gaagccgata ttgtggataa gtacgacaac accatccaca aaaccgacca aatgattcaa
     4921 accgtattcg agcagctgca aaagcagcct gacggcaact ggctgtttgc ctatacctcc
     4981 gatcatggcc agtatgttcg ccaagatatc tacaatcaag gcacggtgca gcccgacagc
     5041 tatctcgtgc cgctagtgtt gtacagcccg gataaggccg tgcaacaggc tgccaaccag
     5101 gcttttgcgc cttgcgagat tgccttccat cagcagcttt caacgttcct gattcacacg
     5161 ttgggctacg atatgccggt ttcaggttgt cgcgaaggct cggtaacggg caacctgatt
     5221 acgggtgatg caggcagctt gaacattcgc gacggcaagg cggaatatgt ttatccgcaa
     5281 tgagtggcgt aaaaaccaat aaagacaaat ttagatgatg tcggggaaga tgcccgaccg
     5341 acaagactat gcaaaatatg aaaaaccaag tacgcggatc aggcatggat gcccgatcca
     5401 atccggccaa tgtttcagac ggcctgcaaa acagttcggg tcatatcggt accaacacgc
     5461 gttaccgcct gaccaaactc ggcgaacaga tagcgcgcct acccatcgac ccgaaaatcg
     5521 cgcgcatttt gctggcggcg aagaaacacg actgcatggc ggaaatattg gtgattgcgt
     5581 ccgcgctgtc gattcaagac ccgcgcgagc ggccgctaga agcgcgcgat gcctcagcca
     5641 aggcgcacga gcgttttacc gacaagcagt ccgatttcct tgcctatctg aacatttggg
     5701 acagcttcca gcgcgaacgc gataaaggct tgtccaacaa gcagctggtg cagtggtgcc
     5761 gccaatattt cctgtcgcac ctgcggatgc gcgagtggcg cgagctgcac caccagcttg
     5821 cccaaaccgc gattgaaatg ggtttaacca ccaaggaagc cgctttcaga cgacctcccg
     5881 aagtcaggca gctcacgtcg tctgaaaatg cgggtgacca agacctatct gctaaactca
     5941 aacaaaaaca actggataaa aagcaacacc gcgcccaaat ccgcgccgcc aaagaagcgg
     6001 gctacgaaca aatccaccgc gccctgctca ctggccttat cgccaacgtc ggcatgaaat
     6061 cgcccgacgg taacgactac accggcgcgc gcggcagccg cttccacctt ttccccgcct
     6121 ccgccctgtt caaagccaaa cccaaatggg tgatggcggc agaattggtt gaaaccacgc
     6181 gcctttacgc gcgcgacgtc gccgttatcc agcccgaatg gatagagcag gaagcgccgc
     6241 acctcgtccg ctatcattat ttcgagccgc attgggaaca aaaacgcggc gaagtcgtcg
     6301 ccagcgaacg cgtgacgctt tacggtctga ccgtattgcc gcgccgcccc gtgtcttacg
     6361 gcaaagttgc ccccgaagaa gcgcgcgaaa tctttatccg cagcgcgttg gtggcgcagg
     6421 aatgcgattt gaaagcggat ttttttgtcc acaacaaaaa gctgattaaa gaaattaccg
     6481 aactcgaaca caaatcgcgc aagcaagacg tgctggtcga tgacgaagcc ctgtttgcgt
     6541 tttataacga acgactgccc gaaatggctt ggaaagacgc gcaaggcagc gtttggggaa
     6601 gtgaagattc cgtacggatt attgaatctg acaaagccga gaggtcgtct gaaaatgagc
     6661 gcaacgagtt tcgtaaaaac aagcgtaatg ggtctcgcca aaatgaaaat cacggcaaca
     6721 ccgtaggttg ggttgaaaac ccaacatcag ccgcaactgc aaaaactgtt gggtttgaca
     6781 atccaaccta cgctgcccaa caaaccaccc cctcccccgt gggggagggt cggggagagg
     6841 gcaaaacagt tgccgcacaa accaactttt ccgcaacctc aacaaaccct ctcccgcagg
     6901 agagggaaca gagtgccgca gcttcaacga tttcagacga cctgcgtcct gcaaatctgc
     6961 agcaaaccgc cccctccccc gtgggggagg gctggggaga gggcaaaaca gttgccacac
     7021 aaaccaactt ttccgcaacc tcaacaaacc ctctcccgca ggagagggaa cagagtgcct
     7081 cagcttcaac gttttcagac gacctgcgtc ctgcaaatct gcagcaaccc tctccctccc
     7141 ccgtggggga gggctgggga gagggcaaaa cagttgccac ccaaaccaac ttttccgcaa
     7201 cctcaacact ttcagacgac tccaaaccca aaaagcagcc tgcaccccaa aaaaaccgtc
     7261 tgaaacccct acccctcgcc gacatccgca ccttccaagc ctggctcaaa accgccgaac
     7321 gcgacaatct gcgcctgctg ttcctcagcc gcgacgattt gatgcaacac gccgccgcac
     7381 acattaccga agaacagttc cccaaattct ggcaaaccgc agacggcaag ttcaaacttt
     7441 cctaccgctt cgagccgcac catccgctag acggcgtgac catgaccgtg ccgctgaccg
     7501 tcctcaaccg cctgcacgcg ccgtcgctcg aatggctggt gcccggcatg atacgcgaaa
     7561 aaatccagtt gcaaatcaaa gcactgccca agcaaatccg ccgcatctgc gtgcccgtac
     7621 ccgaattcat cacccaattt ttaagccaaa accccgaccg caacgccccc atcctgcccc
     7681 aactcgccca agccatcgcc aaaaccgcag gcgacatccg catattcgag caaatcaacc
     7741 aagacgaatg ggccgcgttc aggctgcccg aacactgcta tttcaacctc cgcattatcg
     7801 acgacggcgg acaagagctt gccggcggcc gcaaactgca cgaattgcaa caacaactcg
     7861 gtcaagctgc cgccgttacc ttccgtgaca acacccaaga atttgagcgc gacaacgtca
     7921 ccgcatggga catcggcacc ctgcccgaat ccatcaaatt cgcacgcggc aaacaacagc
     7981 tcaccggcta tctcggccta caaaaagaaa aagacggccg catcgccctg cgcctgtttg
     8041 ataccacaga agccgcagag caggcacacc gtcaaggtgt catcgaattg atgaagctgc
     8101 aattaaaaga gcaggtaaag gatttgaaca aaggcatcca aggcttcacc caagctgcca
     8161 tgctgctcaa acacatcaac gccgacactc tgcgcgacga cctcacccaa gccgtctgcg
     8221 accgcgcctt tatcggcgaa gacgagctgc cgcgcaacga aaaagccttc aaagaacaaa
     8281 tcaaacgcgc ccgcagccgc ctgcccgccg tcaaagaagc cctcagccgc tacctgcagg
     8341 aaaccgccgc cgtctacgcc gaactcaaca gcaaactcgg caaacaccca ttgacccacc
     8401 ttctaagact acgcctgcaa accctgctcg ccgccggctt cgccacccga accccgtggg
     8461 cacaatggcc gcgcctcccc atctacctca aagccatgac cctgcgcctc gaaaaataca
     8521 gcagcaaccc cgcccgcgac gcagcccgcg aagccgatat ccaagagctg gaacaaatgt
     8581 ggcaggaaaa aacagacagc ctgattaaac aaggtctccc catttcagac ggcctcgccg
     8641 cgtttaaatg gatgattgaa gaattgaggg tgtcgctgtt cgcgcaggaa ttgaagacac
     8701 cgtatccggt gtcggtgaag cggctgttga aagagtggga aaaaattgaa aaataaaaaa
     8761 acagcctgaa aagtttcagg ctgttttttt atttgactaa tcgaagtttc ctatatctat
     8821 ttaagtccct ctcaactaat ccaaaagtta aatcagcaac atctttgggg gatacgttta
     8881 aattttcagc aatctgttca ataccaatgc catcattttt taaaatagta agcattttac
     8941 gtaatgcgct tgatatttcc ctttccattg gctctggttc gatagttcga tattttttct
     9001 ttgcaaacaa aggacaaaga ttgtgtatat acatcctatc agtaatcatt cctaatttat
     9061 gcatccgata tgctaaggca acaagtgata caccaaatcg tcttttgatt tttaataaat
     9121 tttcaatagt gataggaaca tgacgatata agcgtagtgc agcctccggc attaaaaaag
     9181 ctgaagcaaa ggcattagcc tctttttcga taatatcacg aggttcatct tctgtaattt
     9241 cactattttt actatgttcc atactgtatt tatcacggat taagtgccct aattcatggg
     9301 cagcatcaaa tcgactacgt tctgcagatt tttgtgtatt taaaaataca aatggatgat
     9361 tttcatacca agtacaaaag gcatcaatgt cctttgtatc taaagataat gaaaatacac
     9421 gaacaccctt agcttcaagt agggtgatca tattcggaat aggttcattg ccaagccccc
     9481 attctaatct tagttcctga gcagcctctt caggagaaat atcagaaaaa tcaggcaata
     9541 cggcttgact tagtgtaaat tctgtctcga gccagtcatt taacaaaaaa gccgtaatgc
     9601 tatgatttaa tgcttgtttt tcaagcctct tcgaggtgcg tgaacgagca cgaaaactta
     9661 ctgcctgaga tttcaactca ggcagtcttt cgtcattagt aaagaaatga actggaaact
     9721 ctaataaatt ggctaattca tttaaatcag gtatttgctc atcttttaca tagtttctaa
     9781 cctgtcgagc ggtaatacct aataactcag ctaattttgt ttgcgtacaa ccacgtttat
     9841 ccagcgcaaa ttccagtctc tcacgattaa atgtctgcat gatttatcat tcaaattatc
     9901 ctgctttttg ttttctttca accaaaggct catattcttc aacaggttgc ttacgttcaa
     9961 gctcatcaaa tttagttaaa tcaacatcag ctaatataat tcgctgcttg tacccagtta
    10021 tttgatgact aacaaaacca ctcggtaaag ataattcaag ttgcacttta ttatacttcc
    10081 agtgaaacag cagaacccaa aactgcacag tatcaggcaa atctagtttt gaattacgaa
    10141 tagcctcctc aaatccctta cctttccttg cggttgtcat tggcatcccg tgatgcctac
    10201 caacatctga agtagcagta gccacaataa tacttttagt tcgacatggc gagagacata
    10261 gaaatgcacc acccgaccaa ggctcaagcg tccagccatc tttacttaaa tataccctta
    10321 gagcaaatgt aatttctgct tgccgataca tccccaatgt atttctgtca gataatgctg
    10381 ctttgtcctg aatattatta tgcgcagtaa gtacaatctc tttaagcatc tcctgagata
    10441 agtacttgct gatttcactt aaagcaatat cactattttg ttgctcgact atttctccta
    10501 cttcaaatgg gaaaggttct gataatgcaa attccaccat aaaaatttcc taattttata
    10561 cgtaatgttt acacaatata tcaggaaata tgaaaacgta caactatatc tataaagcaa
    10621 ttaataagta gcctgcccaa ccgtgtcctt atctttcggc acacccgacc tgcaaatcac
    10681 gcaaaacttg gaatccgtgt gtagggtgtg tgcggtacat acgcacgcag tctttttaaa
    10741 ccacagccct tcccaactaa accaaaaggt cgtctgaacc ctattttcag acgacctttt
    10801 gccactttgt aaaacaaatc ttcccaccat cctctcccca aacatcgccc gaaccagtaa
    10861 acttctcata tttcaacaac tccttggaag caaaccatgt ctggtatcta cctaccccgc
    10921 ctattcccgc cccatatcgc cgaacgcggc ctgttgtatt ttcagcaggg caaggttctc
    10981 gatgtccgaa aaacttccgc cgggcattat cgggcggagg tgtgcggttc ggaaaactat
    11041 tgggtatagt tgaagctgga tagtgatttg tatattaaag acgaaggctg caattgtcct
    11101 tatatctaag agtgcaaaca taccttaaat tactatattg cataggcaaa atacaagcct
    11161 ataacgaatt ggaaacaaaa tgccgtctga aaacatcttc agacggcatt ataaaatctg
    11221 ttcacctttt cagatgagta atgtacaccc ttatacaatt tttgctacta tgccccataa
    11281 atccacggct aaagatatcc ttattatgtc ctatgattta tcgaaacgac ttgtaatcgg
    11341 cttagcatca agtgccctat tcgacttatc cgaatcggat aatatattta gaatggaagg
    11401 ggcagaaacc tataggcaat atcagagaga aaaacaaaac catcccctaa aaaaggcgtt
    11461 gtctttccat ttattaaaaa acttctgtca atcaatgaaa taaacccaaa cgacccaacg
    11521 attgggttta ttcttttatc cagaaacaat ccagatacag attacgagtc ataactatag
    11581 gcttaatatt acacgattct cattccatca aggcggaaaa ccgcacaaat actgaaacac
    11641 tatcgatcga tttgtaaaca agcctactta agtaacttgc agtccttatc atttccttta
    11701 aaataatcca gcccgtcact acacgaactg gcggacttct tgcaaataaa ggttactaga
    11761 ttttcattca tcttaataat aaaaggattt ttatctttat ctatggctac cgccttcaac
    11821 atgaatttac tgtctaaagc cccgcgcgcg attccattca aacggataca aaagccttct
    11881 gcctctttaa tcggcaaact tggccacttg gtagatgttt gtttaaacct cccattctgc
    11941 agataaaact tttccataaa atgtgcattt tctaacaagg ctgcccgcac tgcatttatc
    12001 tttgctttct caacataatt gcgatagctc ggataaacaa ttaaagcaag tacagacaat
    12061 atcaagacca ctgatattaa ttcaaccagc gtaaaccccc gattatcagt cattacttta
    12121 cttccaataa gaacagatta ttcaacatat ttctttgaac agacttacta tcccattcaa
    12181 cagtatgcat atttcccact ctatttttta gcggccggta tagc
//
LOCUS       DAWHRA010000056        12211 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWHRA010000056 DAWHRA010000000
VERSION     DAWHRA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12211
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.056"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            97..813
                     /locus_tag="WIB30_001124"
     CDS             97..813
                     /locus_tag="WIB30_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ3797201.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="WIB30_001125"
     CDS             962..1864
                     /locus_tag="WIB30_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ3797202.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1907..2680
                     /locus_tag="WIB30_001126"
     CDS             1907..2680
                     /locus_tag="WIB30_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ3797203.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEVGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="WIB30_001127"
     CDS             2792..4480
                     /locus_tag="WIB30_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ3797204.1"
                     /translation="MLNKDQFADNHFIRTIIEEDLESGKHTAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMHRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="WIB30_001128"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="WIB30_001128"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ3797205.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWQLFHTLSGELSV
                     NGGLVTAQASAFEFLGAKNDDDHEWFKAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFIAPMLVTTRSADVRVFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELGGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            5841..6224
                     /locus_tag="WIB30_001129"
     CDS             5841..6224
                     /locus_tag="WIB30_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ3797206.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            6517..6990
                     /locus_tag="WIB30_001130"
     CDS             6517..6990
                     /locus_tag="WIB30_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ3797207.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            7116..8219
                     /gene="mnmA"
                     /locus_tag="WIB30_001131"
     CDS             7116..8219
                     /gene="mnmA"
                     /locus_tag="WIB30_001131"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ3797208.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            8290..9960
                     /locus_tag="WIB30_001132"
     CDS             8290..9960
                     /locus_tag="WIB30_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ3797209.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETVSFQTVLKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            10072..11706
                     /locus_tag="WIB30_001133"
     CDS             10072..11706
                     /locus_tag="WIB30_001133"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ3797210.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSENIEK
                     NKGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESTDLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            11721..11923
                     /locus_tag="WIB30_001134"
                     /pseudo
     CDS             11721..11923
                     /locus_tag="WIB30_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11934..>12211
                     /locus_tag="WIB30_001135"
     CDS             11934..>12211
                     /locus_tag="WIB30_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3797211.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDRVIDWQLIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCR"
BASE COUNT         3037 a         3310 c         3210 g         2654 t
ORIGIN      
        1 atttgtgcaa tgctttaatt cctctgacac gatttgaaac acagccgcct taaaacatct
       61 taaaaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccctgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc cggcaaaaag
      241 ctgaccgaat ccgaactttg ccgtcagatg gtgtgttcgc gcaacaccgt ccgcggcgcg
      301 ctgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc cggcctgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgataa ggcgcgaaga agaggcttcc ggcaggggcg accgcgtcgg ctggaaccgc
      541 ctgtccaatg ccttccacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggaacgct tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca catatcatgc ggcaataaag gagggcagga
      961 gatggacagc ctgatgacat tgctttcggt attgataccg atgttcgccg gattttttat
     1021 ccgtgttccc aagccttacc tgcccgcttt ggacaaggtg ctgtcggttt tggtgtatgc
     1081 cgtgctgctg ctgatcggcg tatcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcggggcga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggcgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcattcgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaagcgcg ggcatgtatt gtctgatgct
     1381 gctggtgttc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcgggcgg
     1501 gctgctgttt gccgcatcgg cagacggcgt gtcgtggacg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtgatgacc gaggcttacg gcgcggtatg
     1621 gggcagtatc gcgcttttga acgatttggc acgagagctg ttcgcgctgg catttattcc
     1681 gctgctgatg aagcgttttc ccgatgcggc agtgggggtc ggcggcgcga ccagtatgga
     1741 tttcacattg cccgtgattc ggggtgcggg cggcttggaa gccgtaccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata tcgccgctcc gtttctgatg gtggtgtttt ccgctttggg
     1861 ctgaacgcgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgagttgga
     2041 ggaggtcggc agcctgaaac gccatcacgg cggcgcatct tcgggcggaa acttgccgga
     2101 gggcctgccc gccgaccgcc aaacccggtg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa cacatccccg acggttcgtc gctgtttgtc agtatcggca cgacgatgga
     2221 ggccgtggtg tcggagctgg tcaggcggcg cggcagcctg agggtgatta ccaacaatat
     2281 ccacgccgcc tcgattgctt cggcgcggac ggattatacg gtcatcatca cttccggagt
     2341 cgtccgccct ttggacggcg gtattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaatgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgct tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat tcgcggacaa
     2821 ccactttatc cgcaccatca tcgaagaaga cctcgaaagc ggcaaacata cagctgtcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggctt atatttatga cggtttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagacg ttgagtggtt
     3061 gggcttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttggtta aagacggcaa agcgtatgtc gatgatttga cgccagaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcaggcaaa aacagccctt atcgcgaccg
     3241 cagtatcgaa gaaaacctcg acctgttcac gcgcatgaaa aacggtgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatccggc aacatcaata tgcgcgatcc
     3361 ggtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgcatcta
     3421 tccgatgtac gactacacgc attgtatttc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctc gaatttgaag cccatcgtcc gctgtatgac tgggtgttgg acaacatccc
     3541 cgcgccgcac gccacacgtc cgcgccaata cgagttttcc cgtttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacca tttccggtat gcaccgacgc ggctacacgc ccgaaggcct
     3721 gcgtctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaactccgcc ccgcgcctga tggcggtgtt
     3841 gaacccgctc aaagtaaccc tgaccaactt tgaagccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aggaaatggg cgagcgcgaa gtacctattt cacaaaccat
     3961 ctatattgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtggttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tcccgaaggc cgcaaagtca aaggcgtaat tcactgggtg tctgccgaac acgcagccga
     4201 aatcaaagtc cgtctgtacg accgcctctt taccgtcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagacggc gaatacctgc cgttcaccga tttcctcaat ccggaatccg tcaaggaaat
     4321 caccgcctac gccgaacctg ccgccaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgtaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ttgccgtctg
     4501 aatattgttc ggacggcatt tcccctttgc ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctac
     4621 aaagacacca cctacgccat gatgcgcgag atggcaaaac gcggctggca actgtttcac
     4681 acattgagcg gggaattgtc tgtaaacggc ggtctggtaa cggcacaggc atcggcattt
     4741 gaatttttgg gtgcaaaaaa cgatgatgac catgaatggt ttaaagcggc ggacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccaccca attactgacg ctggcggaac agcagggcgc gaaagtcttt
     4921 aacagcggac aggcgatgcg tgatttcaat gaaaaactgg cgattttgaa cttcagccgc
     4981 tttatcgcgc ccatgctggt aacgacccgt tccgccgatg tccgcgtatt tttgaaagaa
     5041 cacggcgaca tcatcattaa accgctcgac ggcatgggcg gtatgggcat cttccgcctg
     5101 accgaaaaag accccaacat cggcagcatc ctcgaaaccc tgatgcagct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtatcc cgcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg gcgcggcgtg gcgcaggaat tgggcggacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgctggc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatc
     5461 atgaaacaaa aaggtttcga tgtggcggca atgtttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgtcgt ctgaaaggct tttgcttcac aaccgtttgg tttgttcggg
     5581 caggcagggt tttccctcag tcggcagaga tacgcttaat gccgtacgga cacaccgccg
     5641 ccccgttttc agacggtata tatagtggat taacaaaaat caggacaagg tgacgaagcc
     5701 gcagacagta caaatagtac ggaaccgatt cactcggtgc ttcagcacct tagagaatcg
     5761 ttctctttga gctaaggcgg ggcaacgccg tacaggtttt tgttaatcca ctatattgag
     5821 aacattttga aaggataccg atggaacctt cctcctacgc ggcagaaaaa aaaggcaaag
     5881 gcggcatcag gcgcgtcatt aacgcattcg gctattcgat agacggcatc gccgccgcct
     5941 accgttacga agcggcattc cgtcaggttt tgtggctgaa cgtactgctg gtgtgcgcgg
     6001 catttttttg ggtttccgaa accgccgtcc gcctgccgtt gattatcgcg tcttttgtgt
     6061 cggtcattgt cgaactgttc aacaccgccg tcgaagctgc cgtcgatcat acttcgaccg
     6121 aaaaacacga gctggccaaa cgcgccaaag acgcaggttc tgccgcacaa ttgttcgcga
     6181 tgctgatgtt ggcggcggtt tggctgtctg ccctgttcgg gtaaaacgct tgcagcagga
     6241 tttgtaatcc tttaggatta gtatgtggta tttattattc acttaatcta catcaaattt
     6301 ccaggcagta tttgtatgta tagtggatta acaaaaatca ggacaaggcg acgaagccgc
     6361 agacagtaca aatagtacgg aaccgatcca cttggtgctt cagcacctta gagaatcgtt
     6421 ctctttgagc taaggcgagg caacgctgta ctggtttttg ttaatccgct ataagattaa
     6481 gcacattccc cgtctgatat tataaggagc agaaagatga aaaaattatt ggcggccgtg
     6541 atgatggcag gtttggcagg cgcggtttcc gccgccggag tccacgttga ggacggctgg
     6601 gcgcgcacca ccgtcgaagg tatgaaaata ggcggcgcgt tcatgaaaat ccacaacgac
     6661 gaagccaaac aagacttttt gctcggcgga agcagccccg ttgccgaccg cgtcgaagtg
     6721 catacccaca tcaacgacaa cggcgtgatg cggatgcgcg aagtcgaagg cggcgtgcct
     6781 ttggaagcga aatccgttac cgaactcaaa cccggcagct atcatgtgat gtttatgggt
     6841 ttgaaaaaac aattaaaaga gggcgataaa attcccgtta ccctgaaatt taaaaacgcc
     6901 aaagcgcaaa ccgtccaact ggaagtcaaa atcgcgccga tgccggcaat gaaccacggt
     6961 catcaccacg gcgaagcgca tcagcactaa tctgctgaaa atgtttgaaa tgccgtctga
     7021 aaaagcccgg gctttcagac ggcattttta tgcccgcctt taaaatgtgt taaaatccgc
     7081 ctttaaaaac cgccgtttcc aaccatattc tacgcatgaa tacgacagca aacccttcca
     7141 atatcatcgt cgggctctcc ggcggtgtcg attcttccgt aaccgccgcc ctgctcaagc
     7201 agcagggtta tcaagtgcgc ggtgtgttca tgcagaactg ggaagacgac gacaacgacg
     7261 aatattgcag catcaaacag gattcgttcg atgccatcgc cgttgccgat attatcggca
     7321 tcgatatcga catcgttaat ttcgccgcgc aatataaaga caaagtcttt gcttattttc
     7381 ttcaggaata cagtgcgggg cgcacgccga atccggatgt gttgtgcaac gccgaaatca
     7441 aattcaaatg ctttttggac tacgccgtag ggcagggcgc ggataccatc gccaccggcc
     7501 actatgcgcg caaagaagtt cgcaacggcg tgcattacct gctcaaaggt ttggatcgaa
     7561 acaaagacca aagctatttc ctctaccgcc tcaagccttt ccaactcgag cgcgcgattt
     7621 ttccgttggg cggtttggaa aaacccgaag tgcgccgcct tgccgccgaa tttaatttgc
     7681 cgactgccgc taaaaaagac agcacgggca tctgtttcat cggcgaacgc ccgtttcgcg
     7741 agtttctgca gaaatacctg ccgaccgaca atggtaaaat ggttacgccc gaagggaaaa
     7801 ccgtcggcga acacgtcggg ctgatgttct acacgctcgg acagcgcaaa ggtttgggca
     7861 ttggcggcgc gggagaaccg tggtttgttg cggctaaaga tttgacgaaa aacgaactga
     7921 ttgtcgtgca aggacacgac catccgctgc tttatacccg cagccttgtg atgaacgatt
     7981 tgagtttcac gctgcccgaa cgcccgaagg caggacgcta tacctgcaaa acgcgttacc
     8041 gtatggcgga cgcgccttgt gagttgcgct atttggatga tgaaaccgcc gagctggtgt
     8101 ttgacgaacc gcaatgggcg gttacgccgg gtcagtccgc cgtgctgtac gacggcgata
     8161 tctgtttggg cggcggtatc atccaaacga ccgacaaacc cgtcatcatc acacgataaa
     8221 ggtaatgccg tctgaaacgg ttttcagacg gcattgttcc gctcaattcc actttaaaga
     8281 ccgataccta tggaaaaaat ctggttagac agctacgaga agggcgtcag tgccgaaatc
     8341 gacatcacgc aatacaattc cgtcagcgac gtattccgcc aaagcgtgga aaaatttgcc
     8401 gaccaacctg cttttcaaaa catgggtaag acgctgactt atgccgaaac cggcaaactg
     8461 gcaacagatt ttgcttctta tctgcaaaac gtccttaaat tgcctcgagg agagcgcgtg
     8521 gcaataatgt tgcccaatgt actgcaatat ccggttgccc ttttcggcat tttgcaggca
     8581 ggtttggtag cggtgaacac caatccgctc tataccccgc gcgagctgga gcatcagttg
     8641 aaagacagcg gcgcgaccgc catcatcgtt ttggaaaatt tcgccaacac gctggagctg
     8701 gtgctgccgc gcacgcagat caaacacgtc atcgtcgcct ccgtcggcga aatgttcggg
     8761 ctgcttaaag gttcgctgat caatttcgtc atccggaaaa tcaagaaaat ggtgcccgaa
     8821 taccgtattc gggaaaccgt ttcctttcag acggtattga aagagggggc gaagcacgtt
     8881 ttccaacctg tcgcattaaa ccgtgaagat accgcgctgt tgcaatacac gggcggcacg
     8941 acaggtgttg ccaaaggcgc agtgctgagt cacggcaaca tctgcgccaa tatgcttcag
     9001 gcaaaagaat ggattaaaaa ccagttgcgc gaaggcaaag aaaccgttat cgccgccctg
     9061 ccgctgtatc atatttttgc cttaaccgtg aatctgatga tttttgccaa tgccggctcg
     9121 aaaatcatcc tgattaccaa cccgcgcgat atgaaaggct ttatcggaga actgaaaaaa
     9181 cagcgggtta atgtatttat cggcgtgaac acgctgttta acgcgatggt taaccgtcct
     9241 gattttgccg aagtcgattt ttcagaattg cggctgactt tgggcggcgg tatggcgacc
     9301 caaaaagccg ttgccgaaaa atggaaaaaa atcacaggca cgcccatcgt cgaagcctac
     9361 ggtctgaccg aagccagccc cggcgtgtgc tgcaacccct taaacatcga atcatacagc
     9421 ggcagcatcg gtttgcccgt ctcgtccacc gaagtcgaac tgcgcgacgc aaacggcaaa
     9481 gaagtccccg tcgggcagcc gggcgaattg tgggtaaaag gccctcaagt gatgcaaggc
     9541 tactggaacc gccccgaaga aaccgccaaa gccatagacg cgcgcggctt cttggaaacc
     9601 ggcgatattg ccgtcatgga cgaaaaaggc tggttgaagc tggtcgatcg taagaaagac
     9661 ctcgtcgttg tttccggatt caatgtttat ccgaacgaaa tcgaggaagt catcgcgcat
     9721 cacggcaaag tgatggaagt cgcctgtatc ggcgttcccg acgaaaaaac cggcgaggca
     9781 ctcaaagtat tcgtcgtcaa aaaagacccg tctttgacca aagaagaact tatcgccttt
     9841 tgccgcaccg aattaaccgc atataaagta ccgaaaaata tcgaattccg agacgagttg
     9901 cccaagtcca acgtcggcaa aatcctgcgc cgcgagttgc ggcaaagtac cgggaaataa
     9961 agaaaagatg ccgtctgaaa acagccgtcc accgttcaga cggcatccgt ccgtttgcca
    10021 gaaccgcgcg cttcacgtta aaatcacgca ttccaatacg ggtatttcat tatgaccaaa
    10081 ttcattttcg taaccggcgg cgttgtctcc tcactgggta aaggtatcgc cgccgcttct
    10141 attgccgcca tcctcgaatc gcgcggcttg aacgttacca tgctcaagct cgatccttat
    10201 atcaacgtcg atcccggcac gatgagcccg ttccaacacg gcgaagtgtt cgtaaccgac
    10261 gacggcgcgg aaaccgacct cgacttggga cactacgaac gctttatcga ttccacgatg
    10321 acccgccgca acagcttcag cacggggcag gtgtacgaaa acgtcatcgc caaagaacga
    10381 cggggcgact acctcggcgg cacggttcaa gtcatcccgc acattaccga cgaaatcaaa
    10441 cgccgcatcc acgaaggcgc ggcaggttac gatgtggcga ttgtcgaaat cggcggcacg
    10501 gtcggcgaca tcgaatcgct gccgtttttg gaagccatcc gccagatgcg aagccagttg
    10561 gggcgcaaca acaccttgtt tgcccacttg agctacgtcc cctacatcgc cgccgcaggc
    10621 gaaatcaaaa ccaagccgac ccagcacacc gttaaagaaa tgttgagcat cggtttgcaa
    10681 cccgacatcc tgatttgccg tatggacagg acaatgcctg cggacgaacg ccgcaaaatc
    10741 gccttgttct gcaacgtgga agaacgcgcg attgtcggca gctacgatgt ggacagcatc
    10801 tacgaatgcc ccgaaatgct gcacgaccaa ggcatcgaca acatcattac cgagcagttg
    10861 cagcttaatg tgcagcaggc ggatttgacc gcgtggaaaa aaatcgtcca cgccatccaa
    10921 aacccgaaac acaccgtcaa aatcgccatg gtcggcaaat acgtcgattt gaccgagtcc
    10981 tacaaatcgc tgatcgaagc cttgaaacac gcaggcattc acaccgaaac cgacgtgcag
    11041 attacctttg tcgacagcga aaacatcgag aaaaacaagg gcgacgtttc catgcttaaa
    11101 gacatggacg ccatcctcgt tcccggcggt ttcggttcgc gcggtgtgga aggcaaaatc
    11161 gccgccgtgc gctacgcccg cgaaaacaac gtgccatact tgggcatctg cctcggtatg
    11221 cagattgcgc tgatcgaata cgcccgcgac gtggcaggct tgaaaggcgc gaattccacc
    11281 gagttcgacc tcaaatgcgc cgcgccagtc gttgccctga ttgacgaatg gcaaactgcc
    11341 gacggcagcg tcgaaacccg cgacgaatcc accgatttgg gcggcacgat gcgtttgggc
    11401 gcgcaagaag tcgaattgaa agcaggcagc ctcgccgcca aaatctacgg cagcggacat
    11461 atccgcgaac gccaccgcca ccgctacgaa gtcaacaaca actacgttcc tacactggaa
    11521 caggcaggtt tggtcatcgg cggtgtatct gccggacgcg aacgcttggt cgaaaccatc
    11581 gaactgccga accatccttg gttcttcgcc tgccagttcc accccgagtt tacctccaat
    11641 ccgcgcaaag ggcatccttt gttcaccgcg tttgtcaaag ctgcgttgaa caataaaaaa
    11701 gcctgataaa gcgttacttg atgatcaaaa tgccgtctga aagcctgaat gaagactttc
    11761 agacggcatt ttcgcaaatc ggggattata aaagccatca atttaatttt tatggtttta
    11821 tctaatattt accaactgaa tattgttaag cggggcgtga aactcaagtt gagacctttg
    11881 caaaattccc caaaatcccc taaattccca ccaagacatt taggagattt ctcatgagca
    11941 ccttcttcca gcaaaccgcc caagccatga ttgccaaaca catcgaccgc ttcccgctat
    12001 tgaagttgga ccgggtgatt gattggcagc tgatcgaaca atacctgaac cgtcaaaaaa
    12061 cccgttacct tagagaccac cgcggccgtc ctgcctatcc cctgctgtcc atgttcaaag
    12121 ccgtcctgct cggacaatgg cacagcctct ccgatcccga actcgaacac agcctcatca
    12181 cccgcatcga cttcaacctg ttttgccgtt t
//
LOCUS       DAWHRA010000057        12044 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWHRA010000057 DAWHRA010000000
VERSION     DAWHRA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.057"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(53..361)
                     /locus_tag="WIB30_001136"
     CDS             complement(53..361)
                     /locus_tag="WIB30_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797212.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WIB30_001137"
     CDS             complement(345..647)
                     /locus_tag="WIB30_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ3797213.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WIB30_001138"
     CDS             complement(721..3084)
                     /locus_tag="WIB30_001138"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ3797214.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQIDAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGNVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3120..3638)
                     /locus_tag="WIB30_001139"
     CDS             complement(3120..3638)
                     /locus_tag="WIB30_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797215.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3645..4709)
                     /locus_tag="WIB30_001140"
     CDS             complement(3645..4709)
                     /locus_tag="WIB30_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ3797216.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WIB30_001141"
     CDS             complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WIB30_001141"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ3797217.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WIB30_001142"
     CDS             complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WIB30_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ3797218.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WIB30_001143"
     CDS             complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WIB30_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ3797219.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WIB30_001144"
     CDS             complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WIB30_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ3797220.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WIB30_001145"
     CDS             complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WIB30_001145"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ3797221.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEQRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9446..9522)
                     /locus_tag="WIB30_001146"
     tRNA            complement(9446..9522)
                     /locus_tag="WIB30_001146"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9486..9488),aa:Val,seq:gac)
     gene            9701..10816
                     /gene="tgt"
                     /locus_tag="WIB30_001147"
     CDS             9701..10816
                     /gene="tgt"
                     /locus_tag="WIB30_001147"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ3797222.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQAKKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRTGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQARFHENRARGTD"
     gene            10826..11908
                     /locus_tag="WIB30_001148"
     CDS             10826..11908
                     /locus_tag="WIB30_001148"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ3797223.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGSWR"
BASE COUNT         3006 a         3135 c         2702 g         3201 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg attatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgaccgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgacgttgcc
     1141 gtctgaaacg atattggcgg cgcgtccggg atgcagggca ggatgtccgg ttttaacgaa
     1201 ctcgaccgct ttgtttttca acagattttc cacatctgcc ttaatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttcggg cattgccgcg ccgtaccaca agccgccgat
     1321 gcgttcgttt tggacaaacc ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtag ccgcgcgccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggcccaa
     1861 acgcaatccg acctgcttcg cttcgggcaa ttcgccttgc gcttccacca tttcgcctgc
     1921 cgcaccgccg caaatctgca acaccaattc ggtagcacgc tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatccg gcgtagtagc
     2401 gcgcgcgttc acgttttcaa tcacacggct gataaagcga ccgcaatcgg caggcgcgtc
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacaccacgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tgcccggcaa cactgcgccc ggcaacgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaacccgc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttcg gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggaggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgaa taggagaatt gcatgttatg ttctcaatta actaattaac tttagatttt
     3121 caagagctag ctaaaccttt ttgaatggct aagtccctat taattacttc tacagaaatg
     3181 ttccataaag aaatcaaccg caaagtatct ttcttattaa attcttgctt tattaattca
     3241 ttcaattgcc caagataatt aagcggcaca tccgggataa ccagtaataa tttgatttct
     3301 atgctacccc attgcaaatt tgaaaaagca ctaggcataa ttgtttcttt gatatcttct
     3361 ctgcctaata ttcccaaaat ttcaacattt aatgcattac accatttttg ataaatctct
     3421 gaaatatatt catcaaaacg ctcaggagat ctttcttgat ttggaatact ttgttttgcc
     3481 tcaacaaata acaaattatc ttcttgcaga tagataaact ctactgtctt aataccttct
     3541 tgccttctct tccggatata tggttctata taaaacaggt tattttttac tagattaaaa
     3601 gttatacctg attcagtgag atttattatt tcactcataa aaacctaacc aatccatgct
     3661 gtttccggag ataaaacacc tatttcttct ctgaacaatc gaatagactc accaataatt
     3721 tcattatccg gcatgctatt ttgtaaattt tcctgcaccc aagtagtctg ttccttttct
     3781 ttgccaggca taaaaacagg aatactttca ctatgctttc tagctaataa acacagcttc
     3841 ttgataacaa aatatgaatg cgtactcata aaaatctgta ttcccaattt ggatttagat
     3901 aaggaaaaca gaatttctag aaattgatta attgctttcg gatgtagatt agcttctggc
     3961 tcatcaataa ataagattga tccaggactc aaaaaccgat ttcctaatag agtgtctaat
     4021 atcccaatct tttttacccc ctctgctgta gaattgatag agaaagtttg tctatctttt
     4081 tcatataccc atgccttctt tttatcattg tagctaacat aaccgccaaa caatgcttct
     4141 agatcctttc tagcttccgc tgcctcaggg taattcctcc cttttgttgg tttaatatcc
     4201 aatgctttta ccaaatcaat gtaagtagca tcaaaaccaa atctcttttc ttcctctgat
     4261 tttttaatta catcaaatag tgatagaact tctttgggtg gtaaaaaaat agagttagcg
     4321 tttattctgg gagaaagttc gtatagctcc ggtttaatta gctttttggt agttctacca
     4381 aaagaaaaca aaactgaaga actatcttct aaagtcatac ttaacttaag aggccgttcg
     4441 tttggatttt cttttcctct tgtaactaaa tcaccaattt cctctacttg gaacgtccaa
     4501 tatagcttat ctcgcaattc ctcagaaaaa tcacgaatat tctttccttt atgagcttcc
     4561 tcatgacttt ttaaaacact atataatgcc tttaaaataa aagttttccc tttgctattt
     4621 tctgcaataa ttaaatttat tttccctaaa ttatcagcct tcaataagct aatcggcccg
     4681 aaattttcaa gccaaaatga tttaatcata taatccccct aatatatatc aacaatattt
     4741 gtacaagaca cattgtgaaa agacagcaac cacagcacca tagtaaacgc atagcaggcg
     4801 gataatggag cgacatagcg tttcatgatt tagctttctg aggtaggcac aggctgcttt
     4861 tttcattttt aggcagccta cacaatcatt ttacaaactg cttcaaaaaa ttcaaatcgt
     4921 tatcaaaaaa caagcgcaag tcgttcacgt tgtaacgcag catagcgaag cggtcgagac
     4981 caataccaaa ggcgaaaccg gtatattttt cagggtcgat attgacgttt ttcaacacgt
     5041 taggatgtac cataccgcaa ccgccgactt ccagccattt gccgttttcg cccataatgt
     5101 cgatttcggc agacggttcg gtgaacggga agaaagacgg gcggaaacgt acttgcaaat
     5161 catcgcgttc aaagaagcga cggataaaat ccgtgaacac tgctttcaaa tcggcaaaag
     5221 ttacgccctc ttctacccac aaaccttccg cctgatggaa cataggcgag tgcgtggcat
     5281 cgctgtccac acggtaaacg cggccggggg cgataatgcg gatgggcggc tcttttttat
     5341 cgagcatata gcggatttga atcggggaag tgtgcgtacg caaaacatcg ccgttttcaa
     5401 cgtaaaacgt atcctgcatc gcacgggcag gatggtttgc agggatgttc agggcttgga
     5461 aattgtgaaa atcgtcttcg atttcaggcc cgtccgccac ttcgaaaccc attccgtgaa
     5521 agagttcgac cacacgttgc aaggtcaggg ttacgggatg caggctgccg ccttcctgag
     5581 cgcgtccggg cagggtaatg tcgagggctt cggcggcaag tcgggcttgc agcttggctt
     5641 cgttgagggc atcgcgtttg gcattaaaag ccgtctgaaa ccggtttttg cattcattga
     5701 tatgcgcacc tatggttttg cgctcttcag gcgacatttg ccccaaagtt ttcagaagtc
     5761 cggtcaactc gccggttttg ccaagataac gggctttgat ttgttctaga gcgttgaagt
     5821 cttgcgcagc ttctactgcg gcaatgcctt ctgcaacgat gcggtttaca ttttccataa
     5881 tatcaagtct gtcaattaat gatataccac ctgaaacaac gaggccgcct gtatagcctg
     5941 acatatcaga ctcttcagac gaccttattt tcttcacggt caatccattg gataaatttt
     6001 ctgtatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgccg tactatctgt
     6061 actgtctgcg gcttcgtcgc cttgtcctga tttaaattta atccactata ttttaacgca
     6121 aatttattta aaaaaataga ctttaaagaa agaaaatcaa tcacatagaa tttctgcaaa
     6181 caaagaaaaa aggaagccgc agcttccttt tcaatttttt ggattaagca gccaaagcag
     6241 ctttggcttt ttcaaccaat tgtgcaaaag cggctttatc gaacacggcc aaatcagcca
     6301 atactttgcg gtcgatttcg atagaggcgc gtttcagacc attcataaat ttgctgtaag
     6361 acaacccgtt ttcacgcgca cctgcattga tacggacaat ccacaattga cggaattggc
     6421 gtttgcgttg gcggcggtcg cggtatgcgt attggccggc tttcattacc gcctgcttgg
     6481 caacgcggta aacgttttta cgacggccgc ggtagccttt ggctaacgcg aagatttttt
     6541 ggtgacgggc acgggcggta acaccgcgtt ttacgcgtgg catattctaa actccttaag
     6601 cgtagggtaa cattttagca acagaagcca aatcgcgatc gtttaccata gaggtaccgc
     6661 gcagttggcg tttgtttttg gtggtctttt tagtcaagat gtggcgtttg aacgcatgag
     6721 cgcgtttcac accgccgtta cccagtactt taaagcgttt tttcgcgcta gacttggttt
     6781 tcattttagg catgggaaaa ctccattcgt tatcggataa ggcattaggg ggttttaaaa
     6841 catcagtttc aaacacttga acccacaata ccacggtttt ttgccgcaat tgaaaactta
     6901 ctccgaagcg gcaatcggag taagcggaga attatagctt tattttttct tcggcgcaat
     6961 catcatcacc atttggcgac cttccatttt gggaaaggac tcgatttgcg ccacttcagc
     7021 caaatcttct tttacacgtt ccaaaagttg cgcgccgagt tgctggtgag ccatttcacg
     7081 gccgcggaaa cgcaatgtca ctttgacttt atcgccgtcg gcaaggaagc ggttaatgtt
     7141 gcgcatcttg atttgataat cgccctcatc cgtacccgga cggaatttga tttccttgat
     7201 ttgcacctgc ttttggtttt tcttggcttc gtcgcgtttc ttggcctgct ggtatttgta
     7261 tttaccgtaa tccatcagtt tacacacagg cggtttagca gttggggaaa tctctaccaa
     7321 atcgacatcc tgcccttcgg ccatagccaa agcttcacga actgaaacga caccaagctg
     7381 ttcgcctgac tcactgatta aacgcacttc tttggcggta atttcgccgt tgattcgtgc
     7441 ttcgcgttct tgagcgatga tgaatactcc tataaaaatt aatgattgac gagggcatca
     7501 gtgatttctt gctgcaattg cgcaatgaaa tcatccaaat ccaaagaacc caaatcttct
     7561 gctttgcggc gtaccgccac tttgttttcc tgcttttctt tatcgccgac aacgatttga
     7621 taaggaaaac ggtattggct gttgtcgcgg attttgtaac cgattttttc gttacgcaaa
     7681 tccaactcgg cgcggaatcc tgccgcctgc aatttggcag ccacttcacg gcagtaatcc
     7741 gcttgatttt cggtgatatt cataatcacc atttgaaccg gcgccagcca taacgggaaa
     7801 gagcctgcat ggttctcaat cagaatgccg ataaaccgct ccaaagaacc caaaatggcg
     7861 cggtgcaaca ttacagggcg agcgcggtca ttgttttcgg ttacatattc tgcatccaag
     7921 cgttccggta agacgaaatc tagttgcaat gtaccgcatt gccaagaacg acccaaggca
     7981 tctttgacgt gatattcgat tttaggcccg taaaacgcac cttcgcccgg caattcgccc
     8041 cattccacgc cgcaggcagt caatgcctcg cgcaaaccct gctctgcctt atcccacacg
     8101 tcatctgaac ctgcacgttg ttcagggcga agagaaagct tgacggatac atcatggaaa
     8161 ccgaactgtt tgtagatgcg aaccaacaat tcattgaatg cgcgcgcttc gctgacgatt
     8221 tggtcttcgg tacagaaaat atgcgcatca tcttgcacaa aaccgcgaac acgcatcaga
     8281 ccatgcaatg caccgcttgg ttcattgcgg tggcaagaac cgaactcggc caaacgcatc
     8341 ggcaaatcgc gatatgagcg caaaccgtta ttaaaaattt gcacatgacc cggacagttc
     8401 atcggtttca ccgcgtattc gcgtttttct gaactggtca cgaacatatt gtctttgtag
     8461 ttgtcccaat ggccggattt ttcccaaaag gttttatcca taatttgagg cgttttgacc
     8521 tctttataac cggcggcgtt cagctcttta cgcatatgct gttcaatcac ttgccacaaa
     8581 gcccagcctt taggatgcca aaacaccatg cccggcgctt cgtcttgcag atggaacaga
     8641 tccaattgct tgccaagttt gcggtggtcg cgcttttcgg cttcttcgat acgttgaata
     8701 taggctttca actcgtcttt tgtcgcccaa gccgtaccgt atatgcgttg cagcatttca
     8761 ttgttgctgt cgccgcgcca gtatgcgccc gccaacttgg tcagtttgaa gtttttcagg
     8821 aaacgggtat tcggaacgtg cggtccgcgg cacatatcga catattcctg atgatgatac
     8881 atccccatcg cttccacttc gggcatatcg tcaatcaggc gcagtttgta ttcttcgccg
     8941 cgctcttgaa aaattttaat cgcctccgca cgcggagtca tgattttgac cacatcatag
     9001 tcttgggcaa tcaattcttt catacgcgct tcaatggcgg caacatcttc cggtgtaaac
     9061 ggtttttccg tggcgatgtc gtaataaaag ccctcttcaa tgacggggcc gataaccatt
     9121 tttgcattag gatagagttg cttgacggca tgcccgacaa gatgggcgca ggaatggcgg
     9181 atgatttcga tgccttcctg atctttcgga gtaatgattt gaacagcaga attttcaaca
     9241 atcgggtcgc acgcatcgac caatttgccg tttaccctgc ctgccaccgc cgccctcgcc
     9301 aaaccgacac cgatagacgc agcaatttga gccacggtaa cgggggattc gtattggcgg
     9361 actgagccgt ccggcaaggt aatgttcaac atcaaaaagc tccaaaacat taaaaaataa
     9421 cggcataaat gccgttatgg gaatttggta ggcacgattg gattcgaacc aacgaccccc
     9481 accatgtcaa ggtggtgctc taaccaactg agctacgtgc cttcaaagaa tttttgcatt
     9541 ttatcgggtc gttttaattt ttgcaagagg tatgtgcatt tctttatgca acattgtggc
     9601 aagatttggg gatgtcgtct gaaaaaccgt gggaattcat ttataatgcc gtcttttaaa
     9661 acatatttcg ccccctgtcg cccaactgac attcaatctt atgcttaaat ttaccttaca
     9721 caaaaaagac ggatacgccc gacgcggcac gctggagttg aaccacggca aaattgaaac
     9781 gccggtattt atgcctgtcg gtacttacgg ctcggttaag gcgatgaacc cgcaaaacct
     9841 gcacgacatc aaggcgcaaa ttattttggg caacacttat catttgtggc tgcgtccggg
     9901 cttggaggtc gtcgaacagt ttggagggct tcacggcttt atcggctggg acaaaccgat
     9961 tctgaccgac tcgggcggtt ttcaggtttt ttctttgtcg gatatgcgca agctgacgga
    10021 agaaggctgt acgttcaaaa gcccgatcaa cggcgacaag ctgtttttat cgcctgaaat
    10081 ttccatgaaa atccaaacgg ttttgaactc ggacatcgcg atgcagctgg acgaatgcac
    10141 gccgggcgaa gcaacgcgcg aacaggcgaa aaaatcgctg caaatgagcc tgcgctgggc
    10201 ggaacggagc aaaaaagcct ttgaagattt gaaaaacccg aacgcgctgt tcggcatcgt
    10261 gcaaggcgcg atgtatgagg atttgcgcga agaatcgctg cgcggtttgg aacagtttga
    10321 ttttcccggc cttgccgtcg gcggtttgtc cgtcggcgaa cccaagcccg aaatgtaccg
    10381 tatgctgcgc gccgtcggtc cgattctgcc ggaacacaag ccgcattacc tgatgggcgt
    10441 aggcacgccg gaagacctcg tgtacggcgt ggcacacggc atcgatatgt tcgactgcgt
    10501 gatgcccacc cgcaatgcac gcaacggctg gctgtttacc cgtttcggcg atttgaaaat
    10561 caaaaacgcc aaacacaagc tcgacaagcg tccgatagac gaaagctgca cctgctacgc
    10621 ctgccaaaac ttcagccgcg cctacctgca ccatctgcac cgcaccggcg aaatcttggg
    10681 cgcacagttg aacaccatcc acaatctgca tttctaccaa gtcattatgg cggaaatgcg
    10741 cgaagccgtc gaacaaggca aatttgccga ctggcaggcg cgattccacg aaaaccgcgc
    10801 acgcggcaca gactgattcc ctgcaatgcc gtctgaagcc gcttcagacg gcattttgcc
    10861 gctttaccct acccgcacta tttttcagaa accgatcatg cttccattct ttccagaacc
    10921 ctccctttcc tatacgcaac aaaaccgtac cgccgttttg ctgttgaacc tcggcacgcc
    10981 cgacgcgccg accgcgcagg cggtacgacc ttatctgaag tcctttctga ccgaccggcg
    11041 cgtcgtcgaa ctgcccaaat ggctgtggta tcccattctg catggtctgg tactgacgct
    11101 ccgccccaaa aaaagcgcgc acgcctatga aaaaatctgg tttaaggaag gttcgccctt
    11161 agaagtttac acagcacgcc aagctgccgc gcttgccaaa cgtatgcccg acctcatcgt
    11221 ccgccacgcc atgacctacg gcaatccttc ggtggcagac gtattgtcgg aactgaaggc
    11281 gcaaggcgcg ggcagactgc tggtcatccc gatgtacccg caatatgccg cttccagcag
    11341 cggagcggca gtagataagg tgtgcgaaca attactgctg caacggaacc aaatgagcgt
    11401 ccgcaccgtt tcgcgctttt atgacgatac cggctacatt gatgcgatga aaaaccacat
    11461 cctccgatat tgggcggaac acgggcgcgg gaaaaaactg atgttgagtt ttcacggcgt
    11521 accgcagaag caccacgacc tcggcgaccc ctatcccgac gagtgccgcc acaccgccaa
    11581 actgcttgcc gaagcactgg aactgaccga agaccaatat gtcgtatcgt tccaaagcca
    11641 attcggcagg gcaaaatggg ttacaccgag cacgcaggat ttgttcggca aactgcccaa
    11701 acagggggta accgagctgg acgtattttg cccgggcttt ttggcagact gtttggaaac
    11761 catggaagaa atcgccctga tggggcggga acagttttat gaagcaggcg gcaaaagcta
    11821 ccgctacatc ccctgcctca acgacaaccc cgactggata gatgcactcg tcgcacttgc
    11881 cgaagaaaac cttggcagct ggcgttgaaa ctgtttgacc catgtctaaa atgccgtctg
    11941 aacgccgtgt tcagacggca ttttcccgct actttagcgt atgatggcat ctatgctgga
    12001 caacaacatc aagtgcaacc ttatagtgga ttaaatttaa acca
//
LOCUS       DAWHRA010000058        12000 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWHRA010000058 DAWHRA010000000
VERSION     DAWHRA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12000
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.058"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(318..1343)
                     /locus_tag="WIB30_001149"
     CDS             complement(318..1343)
                     /locus_tag="WIB30_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ3797224.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKVAKV
                     AATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKDAQISESE
                     LSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNPALKNLSPAQRRDFADNIIA
                     EKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAV
                     GAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFEEAVHALK
                     PGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVNLLRDLHS
                     GAYVDIR"
     gene            complement(1448..3856)
                     /locus_tag="WIB30_001150"
     CDS             complement(1448..3856)
                     /locus_tag="WIB30_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ3797225.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSVEWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGEVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYITAHSLSAGR
                     NKRP"
     gene            3926..4930
                     /locus_tag="WIB30_001151"
     CDS             3926..4930
                     /locus_tag="WIB30_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ3797226.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(4987..5667)
                     /locus_tag="WIB30_001152"
     CDS             complement(4987..5667)
                     /locus_tag="WIB30_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ3797227.1"
                     /translation="MKSRHLALGVAACDSKVQTSVPADSAPAASAAAAPAGLVEGQNY
                     TVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEHVVWQKE
                     MLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQNPEVLKKWLGEQTAFDGKKV
                     LAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTIDLLADKV
                     REEQKAAQ"
     gene            complement(5961..6068)
                     /locus_tag="WIB30_001153"
                     /pseudo
     CDS             complement(5961..6068)
                     /locus_tag="WIB30_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6126..7664
                     /gene="murJ"
                     /locus_tag="WIB30_001154"
     CDS             6126..7664
                     /gene="murJ"
                     /locus_tag="WIB30_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ3797228.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQVSLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            7797..8753
                     /locus_tag="WIB30_001155"
     CDS             7797..8753
                     /locus_tag="WIB30_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ3797229.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            8804..9586
                     /gene="trpC"
                     /locus_tag="WIB30_001156"
     CDS             8804..9586
                     /gene="trpC"
                     /locus_tag="WIB30_001156"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ3797230.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVPLEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIETEVGKLF"
     gene            9651..11951
                     /gene="recQ"
                     /locus_tag="WIB30_001157"
     CDS             9651..11951
                     /gene="recQ"
                     /locus_tag="WIB30_001157"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ3797231.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGRSLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGWDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLGQNAV"
BASE COUNT         2423 a         3359 c         3439 g         2779 t
ORIGIN      
        1 acgatggaaa gattgttgtt gcttcggata aatttctgca gttttaaata accggattcc
       61 cgcctgctcg ggaatgacgg cataggtttt tttgtttttc cgataaatta ccacaaccca
      121 aaatcccgtc attcccgcga aagcgggaat ccagaccttt aaattccagc cattcccgat
      181 aaattcctgt tacttttcgt ttctagattc ccgctttcgc gggaatgatg aatgacggtg
      241 taaaagtaac tcgaaatcca aaaagccata ccgcccggat ttttgccgat cggtatggct
      301 ttttgcttca aaccgcctta gcggatgtcg acatacgcgc cggaatgcag gtcacgcaac
      361 aggttgacgg ttgcctgttc ggctttttgt tggaagatgt attgccgcac ggaattgcgg
      421 atacgttcct gaggtgtgcc ggcatcgcgc acttcgttca atttgatgat atgccagccg
      481 aattgggtgc ggacgggcgc gccgacctgt ccgggtttga gcgcgtggac ggcttcttca
      541 aaggcgggaa ccatcacgcc gtcggcaaac cagcccaaat ctccgccgtt gcccgcgctc
      601 gcgtcttgcg aatattggcg cgccaggctg gaaaagtctg tgccgctgcg ggcctctccg
      661 tagattttgc ggatggtgct ttccgcgccg acggcggcgt tttcgctgtc ggctttaatc
      721 aggatgtgtt gggcgcggta ttggcgcaac ggtgcgcctt cgggcagggt gatgccttgt
      781 ttttgcgcct gctcgaggaa ggcatcgatt tcagcttcgc tcacgcggct gttctgcatc
      841 actgcctgct ggcggacttt ttcggcaatg atgttgtcgg caaaatcgcg gcgttgggcg
      901 gggctgaggt ttttgagggc gggatttttt gcgacgacgg catcgatttc cgcttcgctt
      961 gcttgaatgt tgcggcgttt gcccgcctgt acaatcaggg attggttgac aagctgcatc
     1021 agcacctgtc gggacagctc ggattcgctt atctgcgcgt ctttgggcag gttggctttg
     1081 gcttcggcaa cggcttcggc aagccggcgg cgcgtgatga cttcgttgtc ggcaacggcg
     1141 gcaatgccgt ctgaaaagcg gataccgccc tgctgttgtg cgggtgcggc ttctttttgc
     1201 gccgtggcgg caactttggc aactttggca gctttggcag ctttggcagc tttggcggat
     1261 gcggtttttg ccttttgcgg tgcggcgtgg acatcggctg ctgccagcaa tgcggcggca
     1321 atcatcaggg ctttgatttt catcattctt tccttatcgt ttgaatcctg cctttgatgg
     1381 cgggtttggg gttgtcggtg gaatgccgtc ttgtgcggga acggtgctcc tgccaggttt
     1441 ccgacggtca gggtcgtttg ttgcgtccgg cggaaagaga gtgggcggtg atatagccgg
     1501 gaacggcgac atccatcctg tctgcggggt ttctgccgac actgctgagg tctttcaact
     1561 gaagtgagaa aaagacagcg tttttgtagg tgttttcgcc ggtaacgtag cgttgggcgt
     1621 acacgcccgc gccccagcag ccgcaactgc ttttgtattc cgcacccgcc agcacctcta
     1681 tcggtttttt ggcttcaaaa ccgtagttgt aacggacgac ggccgacagg ttgcgcgtca
     1741 gcggccattg tgcggacagg tcgagctggc tgagtttgtc gtaaaaatag gaaccgtcgg
     1801 acttcaggta gattttttcg ttgcgcccgt atttgtagcg ggcgttcagc actttgccct
     1861 gtgcgggacg gtagcttgca ccgacggcgt agttctcggc gcgtttgtcg ttttggttgt
     1921 agtggatgct gctgtcgagg atgaagcggc tgccgatgct gccggaggca aatgccaccc
     1981 agtcggaacg gttgcgcggt tttttgccga cgctgccgtc aagcatcacc gcatcatcct
     2041 tgaaatagaa tttctgaccg atgccggcgc ggaaacgctc ttcccccgtc gcgccgtcca
     2101 aaatacggct ttgcacggcg gcggaaaggc tgtttgcggt gttaatcctg tcgttgccgt
     2161 aatagaggtt ttcgcgaaag agctgcccgt agccgaagct gctttccgac gaatcgaaat
     2221 tgggcaggtc gttttgggat ttggcaggaa tatagttgta gaacaggcgc ggctcgaggg
     2281 tttgcaggac ttctccgccg aacatccgcg tattccgctc aaaagttgcg ccgctgtcga
     2341 tgttgacaat gggcagagtg cggctgacgc gtcgggcttc ttggctgccg aagcggttga
     2401 ggctgtaata ggtggcgtgc agtccgagtt tgggacggac atagccccag ctgttgctga
     2461 aatcccattt gatgtcggga tagacgacca ggcggctgcc gtcttggcgg ctgtcgtggc
     2521 tgaatcgggt aaattgtgcg gacacgccga tttgcgccct gccggtgttt ttacgccact
     2581 cgaccgaaag gcgcggcatg agggcatacg gtttgtcttt gtagccgctt tggtttgcca
     2641 gcgtctggta tttcagaacc gaaaggccgg cattcaggct gccgcccgcc gccctgccgc
     2701 cataatccag ccatacacgg cggttgaggt tgacgttgcc ggcgatttct ttgttgccgt
     2761 aaaagtcgcg gtagtagccg ctgtcggaga cttggttgaa atcgacaccc gcctgaagcg
     2821 tgtcggaaat gtcgtgccga tgctgccatt tcgcctgata gcggttattc ctgccgcttt
     2881 tcttgtcgtg cggcagccag gtcaggtcgg actggccggc ataatccggc cgcaggtagc
     2941 gtacctgccc gtcaaagacc gcgccgcgtt cgccgatcac gctgggcgcg aacgtggcat
     3001 cgagattggg ggcaaggttg aaataatagg gaacggaaag ggaaacgccg tccgaaccgg
     3061 cggacagtga gggaacaagc aggccgcttt tgcggttgcc gtcaagcggg aagtccgccc
     3121 aaggggtgta gaaaatggga acgccgccga acacgaaggc ggcgtgtttg gcaacgccta
     3181 tgcctttttc ccgatcggct tcgacggaag cggccttgac ataccagccg gcatcgccgg
     3241 cggaacaggt gttgaattgg gtttccgtca gtttgtaatg cccttcgccc aacatttcgg
     3301 cggtgcggct gacgctttgc agccgccgtc cgccgtgttc ggtttccata cggacgttgt
     3361 gcgcttcccc ggtctgctgc tcgagattgt aggtcagggt ttcgccccga atcagcgtac
     3421 cgtcctgttg gagggcgaac cggtcgcctg cggtaacggt gtcgcccgac tggtcgtaat
     3481 ccgcccaatc ggtattgagg gtcgtccggt tgcgttcgac gacgacgttg ccttcggcac
     3541 gcacctgcac ctgcgactgt ccttccacct tgtcggcaac aatgcgcgta tagtcttcgg
     3601 ggatggatgc ttcgccgctg cctcggacgg cggcttcggt tctctcgggg ctgccgcttt
     3661 cgttactgca aaacaggcag gtcgaaccga ggctcaggct ggtaggctgt atgggttcgg
     3721 acacgctccg aacgcttcct ccggcggtcg gattgtccgt ttcctccgcc gcaacggcat
     3781 cggcggcggc gcaatgcgtg ccgaaacaga agcccaatgc cagcaccagt ggtttgagtg
     3841 aaaataaacg agccaaaatc gcccctcaag tcggtttgcc agttagaata gcgtttattg
     3901 taacctgaaa tgctttagta ctgttatgca acggcaaacc gaactgaaaa attggcttca
     3961 gaccatctat cccgaacggg acttcgatct gtccttcgcg gcggcggatg ctgatttccg
     4021 ccgctatttc cgtgcaacgt tttcagacgg cagcagtgtc gtctgcatgg atgcaccgcc
     4081 cgacaagatg agtgtcgcac cttatttgaa agtgcagaaa ctgtttgaca tggtcaatgt
     4141 gccgcaggta ttgcacgcgg acacggattt ggggtttgtg gtattgaacg acttgggcaa
     4201 tacgacgttt ttgaccgcga tgcttcagga gcagggcgaa gcggcgcaca aagccctgct
     4261 tttggaggca atcggcgagc tggtcgaatt gcagaaggca agccgtgaag gggttttgcc
     4321 cgaatatgac cgtgaaacga tgctgcgcga aatcaacctg ttcccggaat ggtttgtcgc
     4381 aaaagaattg gggcgcgaat taacattcaa acaacgccaa ctttggcagc aaaccgtcga
     4441 tacgctgctg ccgcccctgt tggcgcagcc caaagtctat gtgcaccgcg actttatcgt
     4501 ccgcaacctg atgctgacgc gcggcagacc gggcgtttta gacttccaag acgcgcttta
     4561 cggcccgatt tcctacgatt tggtgtcgct gttgcgcgat gcctttatcg aatgggaaga
     4621 agaatttgtc ttggacttgg ttatccgcta ctgggaaaag gcgcgggctg ccggcttgcc
     4681 cgtccccgaa gcgtttgacg agttttaccg ctggttcgaa tggatgggcg tgcagcggca
     4741 cttgaaggtt gcaggcatct tcgcacgcct gtactaccgt gacggcaaag acaaataccg
     4801 tcccgaaatc ccgcgtttct taaactatct gcgccgcgta tcgcgccgtt atgccgaact
     4861 cgccccgctc tacgcgctct tggtcgaact ggtcggcgat gaagaactgg aaacgggctt
     4921 tacgttttaa acccaatcaa aatgccgtct gaaaaccaag tttcagacgg catttttcaa
     4981 acgggcttac tgcgcggctt tttgttcttc gcgtactttg tccgccaaaa ggtcgatggt
     5041 gttcataccg gactcccagt cggcaaattc aactttatat ttaccgccga cgataaccgt
     5101 gggcgtaccg tcgatttgga aggtttcggt cagctcctgc attttgtcgg cgcgcgcctg
     5161 gctttcgggg gactcgtagg cggcaaggac ttttttgccg tcaaaggcgg tttgttcgcc
     5221 cagccatttt ttgaggactt ccggattttg cagcttgatt ttttggttga ccatcgcatc
     5281 gaaaatatgg ctgttcgcca catctttgct gtcggcggca gccatatcga cggcggcggc
     5341 gaggcgtgcc agcgtcagca tttctttctg ccagacgacg tgttcggtac gcaggtacat
     5401 atcgtcttta aaagacttgg cgtgtttgct taaaacaggt tcgaggtggg cgcagtgcgg
     5461 acagaaatag ccgaaaaact caaggacttc gactttgcct gcctgctgtt ggggaatcgg
     5521 gttggcaagg acggtatagt tttgcccttc gaccagccct gccggggcgg cggctgccga
     5581 agcggcaggc gcgctgtcgg cggggacgct ggtttggact ttgctgtcgc acgcggcaac
     5641 gccgagggcg aggtgtctgg atttcatacg gctctccgtg atgttggaaa cagatgttgg
     5701 aaatcaatcg gaatccggct attttattgc atttccgcgt gttgatacag tttgccgccg
     5761 gaaaaggacg ttttcgtttg gggaaaccgc ttcggacggc atcaaacccg atgccgtctg
     5821 aagcggtttc tgtcgtacaa tacgcgccgt cgccccagac ggatacggct gttggggaac
     5881 taatgatgtt ccaacgcaca ggacggcaca taaagcaccg ccctatgcgt tgccctggtt
     5941 cggaagggtt acaccccttt caaatatagt ggattaaatt taaaccagta cagcgttgcc
     6001 tcgccttgcc gtactatttg tactgtctgt ggcttcgtcg ccttgtcctg atttaaattt
     6061 aatccactat caaagtctga tcctgccgcc ctaaagggcg gggtttcaac cgaaaaggaa
     6121 atacgatgaa tatgctggga gctttggcaa aagtcggcag cctgacgatg gtgtcgcgcg
     6181 ttttgggatt tgtgcgcgat acggtcattg cgcgcgcatt cggcgcgggc atggcgacgg
     6241 atgcgttttt tgtcgcgttc aaactgccca acctgcttcg ccgcgtgttt gcggaggggg
     6301 cgtttgccca agcgtttgtg ccgattttgg cggaatataa ggaaacgcgt tctaaagagg
     6361 cgacggaggc ttttatccgc catgtggcgg ggatgctgtc gtttgtgctg gtcatcgtta
     6421 ccgcgctggg catacttgcc gcgccttggg tgatttatgt ttccgcaccc ggttttgcca
     6481 aagatgccga caaatttcag ctctccatcg atttgctgcg gattacgttc ccctatatct
     6541 tattgatttc actttcctct tttgtcggct cggtactcaa ttcctatcat aaattcagca
     6601 ttccggcgtt tacgcccacg ttcctgaacg tgtcgtttat cgtattcgcg ctgtttttcg
     6661 tgccgtattt cgatcctccc gttaccgcgc tggcttgggc ggtttttgtc ggcggtattt
     6721 tgcagctcgg ctttcagctc ccttggttgg cgaaactggg ttttttgaaa ttgcccaaac
     6781 tgaatttcaa agatgcggcg gtcaaccgcg tgatgaaaca gatggcgcct gcgattttgg
     6841 gcgtgagcgt ggcgcaggtt tctttggtga tcaacacgat tttcgcgtct tatctgcaat
     6901 cgggcagcgt ttcatggatg tattacgccg accgcatgat ggagctgccc agcggcgtgc
     6961 tgggggcggc actcggtacg attttgctgc cgactttgtc caaacactcg gcaaaccaag
     7021 atacggaaca gttttccgcc ctgctcgact ggggtttgcg cctgtgtatg ctgctgacgc
     7081 tgccggcggc ggtcggactg gcggtgttgt cgttcccgct ggtggcgaca ctgtttatgt
     7141 accgcgaatt tacgctgttt gacgcgcaga tgacgcagca cgcgctgatt gcctattctt
     7201 tcggcttaat cggtttgatt atgattaaag tgttggcacc cggcttctat gcgcggcaaa
     7261 acatcaaaac gcccgtcaaa atcgccatct tcacgctcat ctgcacgcag ttgatgaacc
     7321 ttgcctttat cggcccactg aaacacgtcg gactttcgct tgccatcggt ctgggcgcgt
     7381 gcatcaatgc cggattgttg ttttacctgt tgcgcagaca cggtatttac caacctggca
     7441 agggttgggc agcgttctta gcaaaaatgc tgctctcgct cgccgtgatg ggaggcggcc
     7501 tgtatgccgc ccaaatctgg ctgccgttcg actgggcaca cgccggtgga atgcaaaagg
     7561 ccgcccggct cttcatcctg attgccgtcg gcggcggact gtatttcgca tctctggcgg
     7621 ctttgggctt ccgtccgcgc catttcaaac gggtggaaag ctgaccgatg ccgatatcct
     7681 ttttcggaaa acgctttttc cgcgcctata ttcgggcgcg catcccccgc gtccgcacca
     7741 gccggacggg ctgccgcatc atcaatgccg tctgaaaaca gaaaaaccga taccttatga
     7801 ttttgacacc gccggacaca ccgtttttcc tccgcaacgg caatgccgac acgattgccg
     7861 ccaaattcct gcaacgcccc gcgcccgcat accgccgaga gctgcttccc gacagcacgg
     7921 gtaaaaccaa agtcgcctac gacttttcag acggcatttc gcccgatgcg ccgctggtcg
     7981 tgctgtttca cggtttagaa ggaagcagcc gcagccatta cgcggtcgaa ctgatgctcg
     8041 cggtacgcag ccgcggttgg cacggcgcag tcgtccattt ccgcagctgc ggcggcattg
     8101 ccaacaccgc tccggtgttc taccacttgg gcgataccgc cgaaatcgcc tttactttgg
     8161 acacgcttgc cgcgcgttac cgtgaaatat acgccgtcgg cgtatcgctg ggcggcaacg
     8221 cgctggcaaa atatttgggc gaacagggcg aaaacgcgct gccgcaagcc gccgccgtca
     8281 tctccgcacc cgtcgatgca gaggcggcag gcaaccgctt cgacagcggc atcacacggc
     8341 tgctctacac gcgctacttc ctccgcacac tgatacccaa agcacggtcg ctccaaggtt
     8401 ttcagacggc atttgccgca gggtgcaaaa cactgggcga gtttgacgac cgcttcaccg
     8461 caccgctgca cggctttgcc gaccggcacg actactaccg ccaaacttcc tgcaaaccgc
     8521 tgctcaaaca cgttgccaaa ccgctgctcc tgctcaatgc cgccaacgac cccttcctgc
     8581 cgcccgaagc gctgccgaca ccggacgaag tgtccgaagc cgttaccctg ttccagccgg
     8641 cacacggtgg tcatgtcggc tttgtcagca gtaccggcgg caggctgcac ctgcaatggc
     8701 tgccgcagac cgtcctgtcc tatttcgaca gcttccgcac aaacaggcgt taacggtttg
     8761 atgctaatat tccccctttc cccggacgaa tacggaacac aatatgaccg acatcctcaa
     8821 taaaatcctt accgccaagg cacaggaagt tgccgcccaa aaagccgccg ttcccctcga
     8881 acacatccgc gcacttgccg cagaagccgc gcccgtccga agcttcatcg gttcgatacg
     8941 cggcaaacac cgcctaaacc tgcccgccgt cattgccgaa atcaaaaagg caagcccgag
     9001 caaaggcctg atccgtccgg acttccgacc ggccgagatt gcacgcgcct atgaaaacgc
     9061 cggagcggcg tgtttgtccg tactgaccga cgaaccctat ttccaaggtt cgcccgaata
     9121 cctcaaacag gcgcgcgaag ccgtatctct gcccgtgctg cgcaaagact tcatcatcga
     9181 cgaataccag gtttatcagg cgcgcgcctg gggggcggat gccgtcctgc tgattgccgc
     9241 cgcactggaa cgggaacaat tggaacgctt tgaagcggtg gcgcacgaat tgggcatgac
     9301 cgtcctgctc gagctgcacg atgaaagcga attggaaaaa tgccgcaacc tgaccacgcc
     9361 gttgtggggt gtaaacaacc gcaacctgag gacttttgaa gtctccctcg accaaaccct
     9421 gtcgctgctg cccgcactgg aaggcaaaac cgtcgttacc gaaagcggca ttacgggtaa
     9481 ggcggatgtg gaatttatgc gggcgcgcgg cgtgcatacc ttcctgatcg gcgaaacgtt
     9541 catgcgtgcc gacgatattg agacggaagt gggcaaactc ttctaaatcc cgatttcaga
     9601 cggcatattg ccgccgaccg accaacaccc ttaccaagca cggaaccatc atgcaccgac
     9661 cgaccgccaa acagattttg cacgaagtat tcggttatcc cgaatttcgc ggcaggcagg
     9721 aggctgtcat caatactttg gcaggcggca ggagtttgat ggtgctgatg ccgacgggcg
     9781 ggggcaagtc tttgtgttac cagattcccg cgctgatgcg cgaaggcgtg gcggttgtcg
     9841 tatcgccgct gattgcgctg atgaacgacc aagtggccag cctgcatgtg gccgggattg
     9901 aagcagcggc agtcaacagc ggcacatcgg cagatgaggc gcgcgagatt gccgacaagc
     9961 ttgcccaagg ccgtctgaaa ctgctttatg tcgcaccgga acgcttggtt accgaccgct
    10021 ttttgcgttt tctcgaccaa caaaccgtca gtctgtttgc cattgacgag gcgcattgtg
    10081 tcagccagtg ggggcacgat ttccgccccg aataccaaca gctcggcatc cttgccgaac
    10141 gctatccgaa cgtcccgcgc atcgccctga ccgctaccgc cgatgccgcc acgcgcgccg
    10201 acatcaagca ttatctgcac ttggacaatg cgcccgaatt tgtctccagc ttcgaccgtc
    10261 cgaatattta ttatcaggtt atcgaaaaaa acaacggcaa aaaacaattg ctggatttca
    10321 tccgcaaaga aatgacgggg caaagcggca ttgtgtattg cctaagccgc aaaaaggttg
    10381 aagatgtggc gcagtttttg cgtgaaaacg gattaaacgc gattccgtat catgctggtt
    10441 tgagcatgga cgtgcgcgag gaaaaccaac gccgctttac gcatgaagac aatattatcg
    10501 tggtggcgac cgtggcgttc ggcatgggca tagacaaacc cgacgtgcgc tttgtcgccc
    10561 atctcgatat gccccagagt gtcgaacatt tctatcagga atcggggcgt gccggcaggg
    10621 acgggctgcc tgccgcgagc tggctgtgtt acggtttgaa cgattgggtg ttgctgcgcg
    10681 aacggattgc cgaaggcaac agcgacgagg tgcaaaagca aatcgaaatg caaaaactcg
    10741 atgccatgct ttccgtctgc gaaaccgccg cctgccgccg cgtactgctg ctcaaacatt
    10801 tcggcgaaga atccgaaccc tgcggccatt gcgacaactg cctgcatccg cccgtacggt
    10861 ttgacggcac ggtgttggtg caaaaattac tcagctgcgt gtaccgcgcc ggacaacgtt
    10921 ttgccgccgg ttacatcacc aaccttttgc gcggcaaaag cgacgattgg atacgcggca
    10981 accggcacga acaactgtcc accttcggca tcggtacgga gttgtccgac aaagaatggc
    11041 gcagcgtcat ccgccagtgt atcagcctcg gctatctcac cgtcaacatt acccgatatc
    11101 aggcattgca actgaccgaa gccgccaaaa aagtcctcaa aggcgaaacc gaagtgatgc
    11161 tgcgtccgct caagcgcgac aagcccgccg cccgcaccct caaagacaac tggctgcgta
    11221 ccgaacgcga agaacgcctg tggcaggcat tgcgcgtttg gcgtatgaaa caggcagaag
    11281 ccgaaggcat ccccgcctat atgattttcg gcgacaaaac cctgcgcgac cttgtcgaaa
    11341 aaatgccgca aaacctcaac gggctgcacg acatctacgg cttgggcgaa gccaaaaccg
    11401 aacgtttcgg acacggcata ctcgaagtct gccgcaacgc cgccggcttc agccgcgacg
    11461 cggtcatccg tccgcaaacc gaacgcgaac aacaactgcg tcaaaaactc gaggcctggc
    11521 ggtatgaaca ggcaagggcg gaaaactgcg ccctgcatgc cgtcctctcc gacgaaagcc
    11581 ttgccgatat gcttgccgcc acgcccgaaa ccgaaaccga cctcgaaggc gtacacggct
    11641 tgggcagcgt acgcgccgcc aaatacggat gggacatcct cgccgtctgc cgtccgtttt
    11701 cagacggcat cgatgaaacc gccaaacaca aacgccgcct gatgcgcgcc ctgattcaat
    11761 ggtgcaacga aaccgcaaaa cacgaacagt ccgaacccta ccgcattctc agcaaagccg
    11821 ccctacgcgc cattgccgcc aaacagccgg aaggtttggc ggagcttgcc gccgtatatg
    11881 gcgtaggcga agaaaaagcc gcacgttacg gtgcggcggt gttggcggtg ttgggacaaa
    11941 atgccgtctg aaaaccgtta ccaagtttca gacggcattg cctctattta aaaattcctg
//
LOCUS       DAWHRA010000059        11902 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWHRA010000059 DAWHRA010000000
VERSION     DAWHRA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.059"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            153..1052
                     /locus_tag="WIB30_001158"
     CDS             153..1052
                     /locus_tag="WIB30_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3797232.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEVEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
     gene            complement(1113..1219)
                     /locus_tag="WIB30_001159"
                     /pseudo
     CDS             complement(1113..1219)
                     /locus_tag="WIB30_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1317..2336)
                     /gene="lepB"
                     /locus_tag="WIB30_001160"
     CDS             complement(1317..2336)
                     /gene="lepB"
                     /locus_tag="WIB30_001160"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ3797233.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFAAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDSSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(2479..4272)
                     /gene="lepA"
                     /locus_tag="WIB30_001161"
     CDS             complement(2479..4272)
                     /gene="lepA"
                     /locus_tag="WIB30_001161"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ3797234.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPAGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(4415..5116)
                     /locus_tag="WIB30_001162"
     CDS             complement(4415..5116)
                     /locus_tag="WIB30_001162"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ3797235.1"
                     /translation="MTLKTVAVIGAMEQEIELLRETMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPECVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            5302..6414
                     /locus_tag="WIB30_001163"
     CDS             5302..6414
                     /locus_tag="WIB30_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ3797236.1"
                     /translation="MTEKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            6448..7425
                     /locus_tag="WIB30_001164"
     CDS             6448..7425
                     /locus_tag="WIB30_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ3797237.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQYMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            7429..7779
                     /locus_tag="WIB30_001165"
     CDS             7429..7779
                     /locus_tag="WIB30_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ3797238.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            7784..8563
                     /locus_tag="WIB30_001166"
     CDS             7784..8563
                     /locus_tag="WIB30_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ3797239.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            8589..8804
                     /locus_tag="WIB30_001167"
     CDS             8589..8804
                     /locus_tag="WIB30_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ3797240.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREAKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQY"
     gene            8905..9216
                     /gene="grxD"
                     /locus_tag="WIB30_001168"
     CDS             8905..9216
                     /gene="grxD"
                     /locus_tag="WIB30_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ3797241.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            9322..9948
                     /gene="upp"
                     /locus_tag="WIB30_001169"
     CDS             9322..9948
                     /gene="upp"
                     /locus_tag="WIB30_001169"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ3797242.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLATELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            9971..10291
                     /locus_tag="WIB30_001170"
     CDS             9971..10291
                     /locus_tag="WIB30_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ3797243.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETWLLKP
                     LSAQ"
     gene            10542..10961
                     /locus_tag="WIB30_001171"
     CDS             10542..10961
                     /locus_tag="WIB30_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797244.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            10982..11743
                     /locus_tag="WIB30_001172"
     CDS             10982..11743
                     /locus_tag="WIB30_001172"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ3797245.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            11758..>11902
                     /locus_tag="WIB30_001173"
     CDS             11758..>11902
                     /locus_tag="WIB30_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis operon protein HemX"
                     /protein_id="HEZ3797246.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQS"
BASE COUNT         2824 a         3002 c         3175 g         2901 t
ORIGIN      
        1 gggttttatc cggcaggacg gttttttgcc ccaacggaaa atagccgacc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt cgggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gtgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg tcagcggttt ggtgtccggg ctgatgcttg
      661 ccctgtcgat ggtgttcgtc cgcaagactc atgaagtcga gccggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgt gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtctt aatattgatt ccatcaaggc agtattggca
     1081 gttttccgtg caaaacctgc gttaccggac tgatatagtg aattaaattt aaaccggtac
     1141 agcgttggct cgccttgccg tactatttgt actgtctgcg gctcgccgcc ttgtcctgat
     1201 ttaaatttaa ttcactatag gtaggaaaat ccgacaacgt tagatccgcc tgcaaaagtg
     1261 aggaatagca aatgccgtct gaaactattt tcagacggca ttcttggctt cctggcctaa
     1321 cggattgccg taccggccct gccgaaatcg ccgaagttca tcaaaatgaa cattgccttg
     1381 ccgacaacca gcttgtcatc cacaaatccc cagtagcgcg aatcggcact gttgtcgcgg
     1441 ttgtcgccca tagcgaaata gcgtccttcg ggaactttgc acacgaaacc gctgctgtcg
     1501 tcggcatatt ggcagtgttc caaaccgctt tgctctatgg aatatccgtt ttcagacata
     1561 atatcggagg tatatttgcc caatacgggc agggaaacgg caggctgtcc ttcttttttc
     1621 agaatattga aggatttgcc gtctagaccg ctgcggaaca tatccgtgtt gtggatttcg
     1681 gaagggtcgg tgtcgtcggg ataacggtat gtgccgttag gaatatcgga agtgggtttg
     1741 ccatttaccg tcaaaatctt atcccgatat tcgaccacat cgcccggaat gccgacaata
     1801 cgcttgatgt aggtcatctc tggctgcaga ggataattaa aaacaacgac atcaccccgt
     1861 tcgattttgc ctgtaggaat aaatatattg tttaaaacgg gtacgcgcag gccgtaggaa
     1921 aatttgccga ccaaaatgaa atcgcccttg atcaggcccg ggcgcatcga gctggatgga
     1981 atttggaacg gttcggcaat aaacgatcgg atgaggaaca ataccaaaac ggtagggaag
     2041 aaactgccga aataatcgcc gaagtggctg ctttccgaga tttcgggatg agtcttcagg
     2101 cggtatttat atacccccca agccgtaccg cacaatacaa cgaaaatcag gaaaacggcg
     2161 gcaaagctca taaacaggga caaagcggca aacacgccga ccgccgtcag gatataggcg
     2221 tattccagac cggaactcca ttcaccgttt tcctgccgct ttttgtcgct tttgaaataa
     2281 aggatgatgc cggcaagcag cgcggcagcc gcgcccgaca ttagcattgt gttcattgtt
     2341 gttccttaat gcttaaaaac ccgcctgtcc gtgcaaccgt tttaaggcgg caaattgcaa
     2401 aatttgtttg cgggcgcgtg cccctgaaat cagggcggtt tgaggggtgt tcccgacgcg
     2461 ccgccctgtg tgccagagtt atttgtcgct gacctgcaaa atcgccaaga acgcgctttg
     2521 cggaatttcc acattgccca cttgtttcat acggcgttta cctgcctttt gtttttcaag
     2581 cagttttttc ttacgcgtaa tatcgccgcc gtaacatttc gccaaaacgt ttttacgcag
     2641 ggctttgacg ttttcgcggg cgataatctg gctgccgatg gcggcttgga cggcgatgtc
     2701 gaacatttgg cgcggaatca gctcgcgcat ttttgatgcc agctcgcggc ctcggtgaac
     2761 cgcgctttga cggtgcacaa tcaggcttaa tgcatcgact ttttcgccgt tgaccataat
     2821 atccagctta atcaaatcag acggttggaa ctctttgaaa tggtagtcca acgaagcata
     2881 accgcgcgaa gtggatttga gtttgtcgaa gaagtccatg acgacttcgt tcatgggcaa
     2941 atcgtaagtc agcatgactt ggcggcccat gtactgcata ttgacctgaa cgccgcgctt
     3001 ttggttacac aaagtcatga cgttgccgac gtattcctgc ggcacaagga tggtcgcggt
     3061 aataatcggc tcgagtatgg tttcgatgct gccgatgtcg ggcagtttgg acgggttttc
     3121 aacttcgatt ttttcgccgc ttttcaacac gacttcgtaa accaccgtcg gcgcggtggt
     3181 aattaaatcc atatcgaact cgcgctccaa gcgttcctgc acgatttcca agtgcaacag
     3241 acccaagaag ccgcaacgga agccgaagcc caatgcctgg gaaacttcag gctcgaattt
     3301 caacgaagcg tcgttaagct gcaatttttc caaagcgtcg cgcaaggctt cgtagtcgtg
     3361 gctttccacg ggataaagtc cggcgaatac ttggctttgt acttcttgga agccgggcag
     3421 cggctcagtg gcagggttgg caaccagtgt aaccgtatcg ccgactttcg cctgacccaa
     3481 ttcttttacg ccggtaatca aaaagcccac ttcgccggct ttgagttctt gtttttgaac
     3541 cgatttcggt gtgaatacgc ccaactgctc aacctgcgtt tccgccttgg tgctcataaa
     3601 gcgcactttg tctttcagtt tgatggtgcc gtttttcact cgaatcagca tgaccacgcc
     3661 gacgtagttg tcaaaccacg aatcgacaat caccgcttgc agcggcgcgt tttcgtcgcc
     3721 ggcaggtgcg gggattttgg caacgatttc ttccaaaacg tcttccacgc cgatgccgct
     3781 tttggcggaa cactgcaccg cgccgaccgc atcgatgccg atgatgtctt caatttcctg
     3841 ctccacgcgt tcggggtcgg cggccggcag gtcgattttg ttcaaaacag gcacaacttc
     3901 cacgcccaaa tcaatcgcgg tatagcagtt cgccacagtt tgagcttcta caccttggga
     3961 cgcatcgacc accaaaagcg cgccttcgca agctgacagc gaacgggata cttcgtaaga
     4021 gaagtcgacg tgtcccggcg tgtcaatcaa attaagctga tacacctgcc cgtcgcgcgc
     4081 tttatagttg agcgcggcgg tttgcgcttt gatggtaatg ccgcgctctt tttcgatgtc
     4141 catggaatcg agtacctgcg tactcatttc gcgcaaatcc aaaccgccgc aatattggat
     4201 gaagcggtcg gcaagcgtcg atttgccgtg gtcgatgtgg gcaatgatgg agaaatttcg
     4261 gatatttttc attagagttg ttttgaatgt cggacagtgg gtttgggaaa tgccgtctga
     4321 acaaacggcg ttgcgtccga atatcgggtg caacgtggaa atagcccgat attctaacgg
     4381 aaaaccgctg ttttggcatg atttgataaa ggttttataa agatttgacg atttctgcca
     4441 ccatttttgc agaacttgcc gccgccgttt tcaaaaattc gtcaaagctg atgtctgcct
     4501 tttcgtccgc cgaatcggaa accgcgcgga tgatgacgaa aggcgtttcc aactgatgac
     4561 aggtttgggc aatcgccgcc gcttccattt ccactgcttt gactttgggg aagtgcttgc
     4621 ggatttccgc cacgccttcg ctgctgtgga cgaagcggtc gccgctgaca atcaggcctt
     4681 gttctaccgc cgcgccttca aacacttccg ccgcctgttt tgctttgccg accaagaggc
     4741 cgtctgaaac aaatacggcg ggcagttgcg gcacttgtcc ccaaacatag ccgaatgcgg
     4801 ttacatcgac atcgtggtgc gcgatttctg tgccgaccac gacatcgccg actttcaaac
     4861 ccttgcccaa accgcccgcg ctgccggtgt taatgacgca ttccggtgcg aattgatgga
     4921 taagccaagc cgttgcaacc gccgcgttga ccttgccgat gccgctcaat gcaagcacca
     4981 tgcgttttcc cgccaattcg ccttcataag cggcaaactt gccgaaagag acggctttga
     5041 cattttccat cgtctcgcgc aaaagctcga tttcctgttc cattgcgccg ataacggcta
     5101 ctgttttcaa agtcatattg ctgacctgct gtgaatttcg gatagaatgc cgaattatac
     5161 acgctaacac ggcaggattg agtggaggcg gtttgtccat gccgtctgaa acggtttcag
     5221 acggcatggc gggtttttgg tagaatggga aggtacagat tgtttgaaga ttgagggacg
     5281 aggatgttta ccgatgaaaa tatgaccgaa aaggaagaac tgttcgcatg gctgcgccat
     5341 atgaacaaaa acaaaggttc cgacctgttt gtgacgaccc atttcccgcc cgcaatgaag
     5401 ctggacggca aaatcacccg catcacggac gaaccgctga cggcggaaaa atgtatggaa
     5461 atcgcctttt cgattatgag tgcgaagcag gcggaagaat tttcatcgac caacgagtgc
     5521 aacttcgcca tcagcctgcc ggacaccagc cgcttccgcg tcaatgcgat gatacagcgc
     5581 ggcgcgacgg cgttggtatt ccgtgcgatt accagcaaga ttcccaagtt tgaaagcctg
     5641 aacctgccgc cggtcttgaa ggatgtcgcg ctgaaaaaac gcgggctggt tatttttgtc
     5701 ggcggcaccg gctcgggcaa atcgacttcg cttgcctcgc ttatcgacta ccgcaatgaa
     5761 aattcgttcg gacacatcat caccatcgaa gacccgatcg agtttgtcca cgaacacaaa
     5821 aactgcatca tcacccagcg cgaggtcggc gtggatacgg aaaactggat ggcggcgttg
     5881 aaaaacacgc tgcgtcaggc gccggatgtc atccttatcg gcgaaatccg cgaccgcgaa
     5941 acaatggact acgccattgc ctttgccgaa acggggcatt tgtgtatggc gacgctgcac
     6001 gccaacagca ccaatcaggc actcgaccgc atcatcaact ttttccccga ggagcggcgc
     6061 gaacaattgc tgacggattt gtcgctcaac cttcaggcgt ttatttcgca acgcctcgtt
     6121 ccgcgagacg gcggcaaggg cagggtggcg gcagtcgagg tgctgctcaa ttcgcccctg
     6181 atttcggagt tgattcacaa cggcaacatc catgaaatca aagaagtgat gaaaaaatcc
     6241 actaccctgg gtatgcagac cttcgatcaa cacctttacc aattgtatga aaaaggcgat
     6301 atttccctgc aagaagcatt gaaaaatgcc gattccgcac acgatttgcg tttggcggta
     6361 cagttgcgca gccgccgcgc gcaaagttcc agccccgatt tggaactgct ctgatggcgg
     6421 tatggatttc cggacggatg gtttgaaatg atttatccgt ggcataatga gcaatggcgg
     6481 cagattgcgg aacattggga gcgtcgtccc aatgcatggc tgtttgccgg caaaaaagat
     6541 acggggaaaa ctacatttgc ccgctttgcg gcgaaggcac tgttgtgcga aacccctgca
     6601 ccgggctgca aaccctgtgg cgaatgtatg tcctgccatc tgtttggaca gggaagccat
     6661 cccgattttt acgaaatcac ccccttgtcg gacgaacccg aaaacggacg caaactgttg
     6721 cagatcaaaa tcgatgccgt cagggaaatc atcgataatg tgtacctgac ttcggtacgg
     6781 ggcggtttgc gcgtgattct gattcatcct gcggaaagta tgaatgtcca agccgccaac
     6841 agtttgttga aagtgttgga agaaccgccg ccacaagtgg tctttttgct ggtcagccac
     6901 gcggcggaca aggttttacc gaccattaaa agtcgctgcc ggaagatggt tttgcccgct
     6961 ccttcccatg aagaggcatt ggcatatctg cgtgaaaggg gtgtggcgga acctgaggaa
     7021 cgtctggctt tccattccgg agcgccgctg tttgatgagg cggacggtgt ccgtgcgttg
     7081 cggattaaac tgttggatat tttggcagaa ccaaggttgt tgaagatttt ggattacgcc
     7141 gcgcttttcg ataaggagaa acttccgctc gccgtatttg tcgggtggat gcagaaatgg
     7201 ctggtcgatt tgggattgtg cctgcaatac atgaaacccg tctattatcc cgcttatgaa
     7261 gacaggctgc ttcagacggt atccggtttc cgtccgcgca atgtatttgc ggcggaggat
     7321 atgctcaaac agcttgcccc ctacgggttt catactttaa atgtcaaaat gcagatcgag
     7381 catctgctca tcaactattt ggaattgaag aaagagaacg ggtgaattat gtcagacgga
     7441 caaaatattc cggcaaaaat gatgtcgttg cagctgaaag acatgaatct gctgtacagc
     7501 tcctacatgc cgtttttgga acacggcggt ctgtttgtgc agaccaacga cgtattttcc
     7561 atcggggacg atattctgct tgccgtagaa atcctcaact tccccaaact gttcctgccg
     7621 accaaagtcg cctggatcaa tcctgcgcgt acttcctcca aacccaaagg ggtggggctg
     7681 gcattcacaa aacacgaaaa ctgcctgaaa gtcaaagacc agatcgaagt cgaactgggc
     7741 aacacaatcg gcggcagcag acctacgttt accatgtaac gccatgcata tcatcgattc
     7801 gcactgccac ctcaattttg aaggtttgaa agaacgcctg cccgaagttt tgtccaacat
     7861 ggaagcaaac ggcgtggggc aggcgctcgc catcagcgtt agcagggaaa gttttgccga
     7921 agtatccgct atcgccgaag tttatgaaca tatttactgc acaataggcg tacatcccga
     7981 cagcaaggaa gccgaagaat tttctattgc ggaaatggtt gctgcagccg cccatccgaa
     8041 agtggtcggc atcggcgaga cgggtttgga ttattactgg tgcaaaggcg atttgtcctg
     8101 gcaacacaaa cgctttgcag accacatcga agcagccaat caaaccggac tgcccgttat
     8161 cgtccatacg cgtgatgcgg cggcggacac cttgtctatc ctgaaagaat gccgggttaa
     8221 ttcgggcgtt atccactgtt tttccgaaga catcggtttt gcccgtgcag caatggattt
     8281 ggggctttat atttctttct cgggaatcgt tacctttaaa aacgcaccct tggttcagga
     8341 ggcggcgaaa tatgtgccgg acgaccgcat tttggtggaa actgatgcgc cgttcctcgc
     8401 ccccgttccc aaacgcggca ggcagaacga accggctttt gtgcgccata ccgccgaaca
     8461 tatcgccaaa ttgcggaacc aaacattgga acaggttgcg gcatatacga cggaaaactt
     8521 ttaccggctg tttaaaaaag tacccgatat gcggaccgtc tgaccctgta ccgacgataa
     8581 ggaaaaccat gaaggcaatt catccgtatg catgtccgcg ctgctgccgg ctgcctgcca
     8641 acacgtttcg gacaggcatg gcaaattccg cttccaaatt ctgcattgcc aaaggcggca
     8701 gacgggaagc aaaaaaagac gaaagcggcg gcggatatgc cctgtgccat ttgccggaca
     8761 gcaggattgt cgaggagtgg gaatatttcc gttcacaata ttgatactgc gcgatatacg
     8821 gcaaatattg tgggaagttt ccgcttttgc gtataatgcg ccctacctga caaattttgt
     8881 caactttatc aaaaggaata agcgatggct tccatccacg accaaattaa agaagtagta
     8941 acgacacacc gcgtcgtatt gtttatgaaa ggtacgaagc agtttccgca atgcggtttc
     9001 tcttcccgcg ccgtgcaaat cctgaacgcg gcaggctgca ccgattacgt taccgtcaac
     9061 gtattggaaa atcccgaagt gcgccaaggc attaaggaat acagcgactg gccgaccatc
     9121 ccccaacttt atgtgaacgg cgagtttgtc ggcggttcgg acatcctgat ggaaatgtat
     9181 gaggcaggcg agctgcaaga gctgctgaaa gcctgatgga ttcggcaatg ccgtctgaac
     9241 gtgtttcaga cggcattttc ttttccggca aatcaaaaaa aagtataatg gcgcgtctca
     9301 aaatcacatt ggaacaccgc gatgaacgtt aatgttatca accatccgct cgtccgccac
     9361 aaattaaccc tgatgaggga ggcggattgc agcacctaca aattccggac gcttgccacc
     9421 gagctggcgc gcctgatggc atacgaggca agccgtgatt ttgaaatcga aaaatacctt
     9481 atcgacggat ggtgcggtca gattgaaggc gaccgcatca agggcaaaac attgaccgtc
     9541 gttcccatac tgcgtgcagg tttgggtatg cttgacggtg tgctcgacct gattccgact
     9601 gccaaaatca gtgtagtcgg actgcagcgc gacgaagaaa cgctgaagcc tatttcctat
     9661 tttgagaaat ttgtggacag tatggacgaa cgtccggctt tgattatcga tcctatgctg
     9721 gcgacaggcg gttcgatggt tgccaccatc gaccttttga aagccaaggg ctgcaaaaat
     9781 atcaaggcac tggtgctggt tgccgcgccc gagggtgtga aggcggtcaa cgacgcgcac
     9841 cctgacgtta cgatttacac cgccgcgctc gacagccact tgaacgagaa cggctacatc
     9901 atccccggct tgggcgatgc gggcgacaag attttcggca cgcgctaact gactgatttt
     9961 cggagttgat atgaattttc aagactatct cgccacattt ccttcaatcg accatctggg
    10021 cggtttggac gttcaggatg ccgacggcaa aacggttcac cacattcctg ccgttcaggg
    10081 taagctcggt tcgctcaagc tgtacaatgc tttggcggaa cgttttgacg gaaaattggg
    10141 taaagaagcg gcagaacagg gtttgatatg gtttgccgaa cacgttgccg acgcgcgtgc
    10201 ccatccgggc aagcatccga acatcgatct gctggaaaat gtcgtgcaaa gcggtgaaac
    10261 ctggttgctc aagccgcttt ccgcgcaata attttcgacc atgccgtctg aaatccgttt
    10321 cagacggcat tttgtcggaa agaagaccgt aaaacgggca ttttcttttc tatttcagga
    10381 tacgggcaat gatgtttcaa cacacaggac gacacataaa gcgccgccct atgtgttgcc
    10441 ctaatttgga aggggttaca cccttttcaa ataaaatctg atgctgctgc cacgaaggac
    10501 ggatgtccga gtggcggggt ttcaaccatt aaggaaatac gatgaaaaaa atgttccttt
    10561 ctgccgtatt gcttctgtcg gctgccgccc aaaccgtgtg ggcggatacg gtgttttcct
    10621 gtaaaacgga caacaacaaa tacatagaag tccaaaaaat caaccgcaat ctttacgaat
    10681 attcgttcgg cagtgcggca aaaaaagaaa ttgccatacg caacagcaaa gctgacctgt
    10741 tggggcgttc cgacaggtgg caaggtatgg gcagcggtcg ttgggcaacg atgaaattcc
    10801 aaaacggcga atttatgtac accatatgga caggcttcga ttccgtgact catacggaaa
    10861 gcagcggtgt cgttgtggag cgtaggggca aggaagtcgc acgggtcggc tgtacgccga
    10921 aaaccgcgca ggcgaatttc aacgatgacg atttttccta gtaatcgggg cggataaggc
    10981 gatggaaaca gcgaaacccg tcatgctgat tgtccgtccg tcgggcaggg ccgcagaaga
    11041 tgtcgaaact tgcctgaatg ccggttggcg cgcggaagta ttgagtccgg tcgaaatcga
    11101 agcagatgct gccggactgg aacttttgtc cgaacaatat gcccgtgcgg atgccgtgtt
    11161 ttgggtcagt ccgaccgccg ttgaaaccgc cgtcccgtac cttaaccttt cagacggcat
    11221 aaaggcgcag attgccgtag ggcagggcag ccgccgcgca ttggaacgct gtttggtcag
    11281 aacggtcatc gcgcctgatg acggcaacga cagcgaggcg gttttgcgcc tgccggtttg
    11341 gaacagtctg cccgaaggtg cgcgcgtatt gtttgtgcgc ggacacggcg ggcgggattt
    11401 tttgatgaat gccttgcagg agaaaggttt tcggacggag gtggcagaag tctatttcag
    11461 acggcataaa cctttgaact ttcaaaattt ccaaaccgaa aatattgccg ccgcctatat
    11521 tacgtcgacc gagctggtgc gcttgctgtt cgggcagctt ccgccgcaat tttcccgatt
    11581 cttcaaatcc ttgctatact ttacccatca tccgcgcatt gcggaggcat tgaagcgcga
    11641 aggcgtgtgt tcggtcgaaa ccgtccctac gctggaagcc gcgctttccc attcttccat
    11701 ttccgtttca gacggcatgg tctttcccgg aacctcaaat taataaggag caaaacggtg
    11761 ggcgaacctg aaaacaaatc atccgaaccc gtacgcgaga tacaggcatc aaaagaaatg
    11821 ccgtctgaaa cctcttcccc acgcaaagaa aacgaaacag aagtacacat tcctgccgct
    11881 ccttttatcg tcaaacagtc cg
//
LOCUS       DAWHRA010000060        11767 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWHRA010000060 DAWHRA010000000
VERSION     DAWHRA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.060"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            222..1580
                     /locus_tag="WIB30_001174"
     CDS             222..1580
                     /locus_tag="WIB30_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ3797247.1"
                     /translation="MKLIYIDPPYNTGNDGFKYNDKFNHSTWLTFMKNRLEIAKELLM
                     KDGSIFVSIDDNEQAYLKILMDEVFGNENFICNFIWEKKTGASDAKQIATITEFVLCY
                     SKNFKTVKLNKNTFSYDTERYKLSDKFEQERGKYYIDNLDRGGLQYSDSLNFAIQCPD
                     GTFTYPNGRTEFVNDGWIWKWSKNKIDWAITNGFLEFRKSKSKKSGWSVCYKNYMLVD
                     NENKPIERSAPYKNLIQDILNTHATDELKKLFGSKVFTTPKPESLLQYLIQIATSESD
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSINEFSQTIQE
                     PEFQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFAGCLNDDDKALSRAFYQTEKKD
                     GE"
     gene            1570..4350
                     /locus_tag="WIB30_001175"
     CDS             1570..4350
                     /locus_tag="WIB30_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ3797248.1"
                     /translation="MANNKMLFEVIENRKAVYLEDGDDEKCRLPEFVERNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIGSTHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQGELDLEKEIND
                     RTSNAEIERKGWEHMVLELLLNKNGNPSENVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFAANKDFRELLIWANKKIPNPNAKTNNANSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDEKVKQLGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRPDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKTLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARL
                     GDLKSQYDIHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(4494..5666)
                     /locus_tag="WIB30_001176"
     CDS             complement(4494..5666)
                     /locus_tag="WIB30_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ3797249.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            5957..6403
                     /gene="iscR"
                     /locus_tag="WIB30_001177"
     CDS             5957..6403
                     /gene="iscR"
                     /locus_tag="WIB30_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ3797250.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6432..7646
                     /locus_tag="WIB30_001178"
     CDS             6432..7646
                     /locus_tag="WIB30_001178"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ3797251.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7907..8293
                     /gene="iscU"
                     /locus_tag="WIB30_001179"
     CDS             7907..8293
                     /gene="iscU"
                     /locus_tag="WIB30_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ3797252.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            8380..8700
                     /gene="iscA"
                     /locus_tag="WIB30_001180"
     CDS             8380..8700
                     /gene="iscA"
                     /locus_tag="WIB30_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ3797253.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(8660..8881)
                     /locus_tag="WIB30_001181"
     CDS             complement(8660..8881)
                     /locus_tag="WIB30_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ3797254.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            8963..9463
                     /gene="hscB"
                     /locus_tag="WIB30_001182"
     CDS             8963..9463
                     /gene="hscB"
                     /locus_tag="WIB30_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ3797255.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKKSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            9561..>11767
                     /gene="gyrA"
                     /locus_tag="WIB30_001183"
     CDS             9561..>11767
                     /gene="gyrA"
                     /locus_tag="WIB30_001183"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ3797256.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGLVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIET"
BASE COUNT         3553 a         2929 c         2605 g         2680 t
ORIGIN      
        1 aaagacaaga aatctttttt aatcaaaccc ttgcttttga tgaaattgat cggctttttg
       61 acgcaaaagc attctcaaaa ttctctcgct ataccgcaga cggcaaacaa gccgttggcg
      121 aaatcaaacg acattcagac ggcacacccg ccgaaaatct cattatcaaa ggcaataatc
      181 tgattgccct gcattcgctt gccaagcagt ttaaaggcaa agtgaagctg atttatattg
      241 acccgccata taacacgggt aatgacggtt ttaaatacaa cgacaaattt aatcattcca
      301 cttggctgac ttttatgaaa aaccgtctag aaatcgccaa agagctgctt atgaaagacg
      361 gttcgatttt tgtgtcaatt gacgacaacg aacaggcata tttgaaaatt ttaatggatg
      421 aagttttcgg aaatgaaaat ttcatctgca attttatttg ggaaaaaaag acaggtgcgt
      481 ccgatgccaa acagatagcg actattacag agtttgtctt atgttactca aagaacttta
      541 aaacagttaa attaaataaa aacacgtttt cttatgatac agagagatac aaattaagtg
      601 ataagtttga acaggaaaga ggcaaatatt atatcgacaa tttagataga gggggattgc
      661 agtatagtga cagtttgaat tttgcaatcc aatgtccaga tggcactttt acgtatccga
      721 atggcaggac tgaatttgtc aatgatggct ggatatggaa atggagtaaa aataaaattg
      781 attgggcaat aacaaacggt tttttggagt ttagaaaatc aaagtctaaa aaatctggat
      841 ggagtgtatg ttataaaaac tatatgttgg ttgataacga aaacaagccg atagaacgtt
      901 ctgctcccta taagaactta atacaggata tcttaaatac acatgcgaca gatgaattga
      961 aaaaactgtt cggcagcaaa gtttttacta ctccaaaacc tgagagctta ttgcagtatc
     1021 ttattcaaat tgccacatcc gaatccgaca tcgtcttaga ctaccatctt ggtagtggca
     1081 caaccgccgc cgttgcccac aaaatgaacc gccaatatat cggtattgaa caaatggatt
     1141 atattgaaac gcttgctgtt gaacgcttga aaaaagtgat tgatggcgag caaggcggta
     1201 tttccaaagc cgtgaattgg caaggtggtg gcgaatttgt ttatgccgaa cttgctccat
     1261 ttaacgaaac cgcaaaacaa cagattttgg cttgcgaaga ttcagacggc atcaaaacgc
     1321 tgtttgaagg tttatgcgaa cgctatttct tgaaatacaa cgtcagcata aatgaattta
     1381 gtcaaaccat tcaagagcct gaatttcaat ctttgccatt agacgaacaa aaacaaatgg
     1441 tgcttgaaat gttggattta aatcaaatgt atgtttcatt atccgaaatg gacgatgaac
     1501 aatttgcagg ctgtctgaat gatgacgata aagcgttaag ccgtgcgttc tatcaaacgg
     1561 agaaaaaaga tggcgaataa taaaatgttg tttgaagtga ttgaaaaccg caaagctgtg
     1621 tatttggaag acggcgatga tgaaaaatgc aggctgcctg aatttgtaga acgcaattta
     1681 aaatatccgt tttttgaatg gcaaaaatcg gctttagaaa actttgtgat ttttgaccgc
     1741 acttcaaaat taaaggattt tcccgatatc aaaaaccgcc ctacccattt gctgttcaat
     1801 atggcaacag gtgcaggcaa aacgatgatg atggcggcgt tgattttgta ttattttgaa
     1861 aagggttatc ggcattttct gtttttcgtc aatcaaaaca atatcgtaga taaaacggaa
     1921 aataatttta ttggctcaac gcacgcaaaa tttttattta ccgagaagat tttgcagggc
     1981 gatacggtaa ttcccattcg caaagtggaa atgttcagcc cacattcaga cggcattgaa
     2041 attaaattta ccagcattca aaagctgtat aacgatatcc acaccgaacg ggaaaaccaa
     2101 accacattgg cggatttgca caaattgaac cttgtgatgc tgggcgatga agcacaccat
     2161 ttaaacgcac aaaccaaagg caaaaaacaa ggcgaattag atttagaaaa ggaaattaac
     2221 gaccgcacta gcaatgccga gattgaacgc aaaggctggg agcatatggt tttggaattg
     2281 ttactcaata aaaatggcaa tcctagcgaa aatgtgctgt tggaatttac cgccacgcta
     2341 cctgaaaatg ctgacgtgca acaaaaatac gcggataaaa tcatcacaaa atttggctta
     2401 aaagaatttt tgcaaaaagg ttataccaaa gaaatcaatt tggtatccag tacgctgggc
     2461 aagaaagagc gggtgttgca cgctttatta tttgcttggt atcgccatca aatcgcgttg
     2521 aaatacggca ttgccaattt caagcctgtg atgttgttta gaagtaagac gattgatgaa
     2581 tcaaaagcgg attattcggc atttttaaat tgggcagaaa atgtacaagc ggatgatttt
     2641 gagtttttag atacgttttc aggcagcctg aataatagcg acaacgccaa cgaacaaggc
     2701 aaaacccgaa ccgaacaagc cctaaaattt atgcaggaaa atggctttga gtttgcacat
     2761 ttggcaaatt gggtgaaaca aaattatcaa aaacataatg tgattattac caactccgaa
     2821 accaacaaaa acaaaactga aaaaaccgac agcgaaaccg aaaaattgct aaacaatttg
     2881 gaagcggctg ataatccaat ccgtgccatt tttacggtag atagattaac cgaaggttgg
     2941 gacgttctga atttgtttga tattgtgcgt ttgtatgaag ggcaaaatgg cggtggttca
     3001 aataaaaaat caggcaaaac ggctgccgct accgtatcgg aaaagcagtt gattggtcgt
     3061 ggcgtgcgtt attttccatt tgcgtttgaa ggtaaacagc cgaataaacg caaatttgac
     3121 aacgatatgc aacacgaatt gcgtatcttg gaagaattgt tttattacac gcacgatgag
     3181 caatcccgct atatttcaga actgaaaaac gagttgcgaa aagacggtta tttgcctgaa
     3241 aaagacgatg ataaggtatt ggcaacattt aaactcaaat ctgaatttgc cgctaataag
     3301 gattttagag aattgttaat ttgggcgaat aaaaaaatcc ccaacccaaa cgccaaaacc
     3361 aataatgcaa acagcctgca agccaatccg caaacgcttt cattccaagt tcacggcaat
     3421 caactgttgc aggaaacgca atttacagcc gatgaaaatg atgaaaaagt caagcaactt
     3481 ggcacgcaaa ataattttac tcaaaccata aaaatgagtg aaatggagcg gcacattttc
     3541 aataaagccc tgcatatcaa agggaaaaat agtcaatctt tattccattt caaccgtttg
     3601 caaagcaagc tcaacattca aaatcgcaat gaattgcaaa ataacttgtt gaaagactgg
     3661 caaattgaat ttttaggatt agggcaagac aaacaggttc gcccggacga taaacttgca
     3721 ggctgcctga aaatcttgga aatggttgaa aaacatctga atgaaagtga tatgccgttt
     3781 atcggtacaa aagaatttac gcctaaaaca ttatgggaaa ttttcggcac accaaaacaa
     3841 aaatgggtca aaaaagatga tgtaaaaacg actattgcca cgcaaaatga ttggtatgtg
     3901 atgaataatt ttgccggaac gggtttggaa gaagcgttaa ttcaatttat ttcagcgcgt
     3961 ttgggcgatt tgaaatctca atatgacatt catttaatcc gtaatgaaga agtgtttaaa
     4021 ttgaataact ttgccgatgg tgaaggattt atgccggact ttattttatt gctgaaagat
     4081 aaacaaaaat cttcttccaa tggcgtgaat gactttttgc attaccaaat tttcattgag
     4141 cccaaaggtg agcatttagt ggaaactgat ggatggaaag aagaattttt agaagccatc
     4201 actgtggaat atgggatgga taagatcctg caaaaagata caccgcatta tcgtttgatc
     4261 ggtttaccgt tttttactga tcatcagaaa aatggacaat ttacggagtc attcccttta
     4321 ggtgcggcat cgcttgaaaa aatgaagtag tgcattgcag gcagccccgt ttgacaaaac
     4381 ttcctttaca aaagggcgtt ttgtcagata tttaatcaac acattattaa aatacagcca
     4441 aattttaatg ccgtctgaac cctgtgttca gacggcatcg tatttttcag tatctaaacc
     4501 gtttccctac cccaatcctt acccctcaaa atcgaggcat cgacatcttg aatatcgcgg
     4561 tgtcccgtaa acgccataga tatatccatt tctttatata ggatttccag cgcacgggtt
     4621 acgccttctt cgccatacgc gcccaagcca tacaggaacg cccgacctat cattgtacct
     4681 ttcgcgccca aagcccaagc cttcaaaata tcctgaccgc tgcggatgcc gctgtccatc
     4741 caaacttcga tgtcgctgcc cactgcgctg acgatgtcgg gcaaggcttt gatggcagac
     4801 acggtatcgt cgagctgtcg gccgccgtgg ttggaaacaa tcaatgcgtc cgcgccgctt
     4861 ttcgctgctt tttccgcgtc ttcgggttcc ataatgcctt tgataatcag cttgccgccc
     4921 cacaaatctt taatgcgcgc cacatcgtcc cagctcaggc gcgggtcgaa ttgttcggaa
     4981 gtccatgaag acagcgaaga caaatcgccg acgttcttcg cgtgtccgac gatattgcgg
     5041 aacgtgcggc gttccgtgtt cagcattttc atacaccatt cgggcttggt cgccagattg
     5101 attaaattgg cgatggtcgg tttcggcggc gcggacaggc cgtttttgat gtctttgtgg
     5161 cgttgcccca aaacctgcaa atcagcggtc aataccaatg ccgaacattt ggcatccttc
     5221 gcgcgcttaa tcaggttttc cataaactcg cggtcgcgca tcacataaag ctgaaaccaa
     5281 aatggtgcgg aagtgttctc ggcaacgtct tcaatcgagc agatagacat cgtggacagc
     5341 gtaaacggaa tgccgaactt ctccgccgcc cgcgccgcca aaatttcacc gtcggcgtgt
     5401 gccatacccg tgaaacccgt cggcgcaatc gccaccggca ttttcacatc ctgcccgatc
     5461 attttggttt ccaggcttcg gccttccata ttgaccaata ccttttgtcg gaagcggatg
     5521 tctttgaaat ccgaagtgtt ttcacggtag gtagtttctg tccacgaacc cgaatcgatg
     5581 taatcgtaaa acatacgcgg cattttgcgc ttggcaacgc ggcgcaagtc ttcgatgcag
     5641 gtcattttgc tcaaatcacg tttcatttgt cgccccctga atacctgaat aactttatat
     5701 gaaatcgata atgtatatca atattgatta taaggcaaat catttcaaca tttgccgcat
     5761 ccgccgcagc tccctacttt aagcgacata aggtttaaaa ttcaaaaata acaaattaaa
     5821 atcaaaatat taaaaatcaa tcaatctatc gatttaaaca gccaatcaca caatccgccc
     5881 tcatacttga ctgaaacact cagatattgg acaattccac ccactaataa aaaaaccgac
     5941 atgggcaacc accaccatga gactgaccac caaagggcgt ttcgccgtta ccgctatgct
     6001 ggatttggcg atgaacgcgc aaaccggcgc cgtcaaactc agtgccatca gcgaacgcca
     6061 aaacatatcc ctctcctatc tcgagcaatt gttcggcaaa ctccgccgcg ccggacttgt
     6121 tgaaagcctg cgcgggcccg gcggcggcta catcctcgcc gcaccggcgg cacgcatcaa
     6181 catcgcccaa atcatcgccg ccgccgaaga ccggctggac gcaacccaat gcggcagcaa
     6241 agccaactgc caccacggcg cgccctgcct gacgcacgat ctttgggaga atttaaacaa
     6301 aaccatcaac gactacctcg gcagcgttac cctgcaaagc atcatcgaac agaaaaacaa
     6361 cggcgacggc agccgcgtcg tccaatttac acacatccat taaataacac ccgaaaaaga
     6421 aagagcaaac catgaccgtc aaaacccccg tttacctcga ctacgccgcc accacccccg
     6481 ttgacaaacg cgttgccgaa aaaatgattc cctatctgac cgaaaccttc ggcaacccag
     6541 cctccaacag ccacagcttc ggctgggaag cagaagaagc tgtagaaaaa gcacgtgcag
     6601 acattgccgc cctgattaac gccgactcta aagaaatcgt tttcaccagc ggcgcgaccg
     6661 agtccgacaa cctcgccatc aaaggcgcgg caaacttcta caaaaccaaa ggcaaacacc
     6721 tcatcaccgt caaaaccgaa cacaaagccg tactcgacac catgcgcgaa ctcgaacgcc
     6781 aaggttacga agtaacttat ctggacgtac aagaaaacgg tttggttgat ttagacgtac
     6841 tgaaagccgc catccgcgaa gacaccatcc tcgtttccgt aatgtgggta aacaacgaaa
     6901 tcggcgtggt tcaagatatt cccgccatcg gcgaaatctg ccgcgaacgc aaaatcattt
     6961 tccacgtcga tgccgcccaa gcctgcggca aagtacctgt tgacgttgaa gccgccaaag
     7021 ttgatttgtt gtctatgtcc ggccacaaag tatacggccc taaaggcatc ggcgcgctgt
     7081 acgtccgccg caaaccgcgc gtccgcctcg aagcccaaat gcacggtggc ggccacgaac
     7141 gcggcttccg cagcggcaca ttaccgaccc accaaatcgt cggtatgggc gaagccttcc
     7201 gcatcgccaa agaagaactc gaacaagata tggcgcacta ccgcaaactg cgcgacatct
     7261 tcctcaaagg catcgaaggc atcgaagaag tctatatcaa cggcgacctc gaacaccgcg
     7321 ccccgaacaa cctgaacgtc agcttcaact tcgtcgaagg cgaaagcctg attatggcag
     7381 tgaaagaact cgccgtatcc agcggctccg cctgcacctc cgcctcgctc gaacccagct
     7441 acgtcctgcg cgcattgggc agaaatgacg aattggcgca ctcgtccctg cgcatcacct
     7501 tcggtcgcat gaccactgaa gaagaagtgc aatttgccgc cgaactgatt aaatccaaaa
     7561 tcggcaaact gcgcgaactg tcgccgttgt gggaaatgtt caaagacggg attgatttga
     7621 actcgattga atgggcggcg cattaaagcg taccaacatg ccatccgaac cttcagacgg
     7681 cattccaaaa acaaagcaat caagagaaaa atatgaacga acaagattta gatttggaca
     7741 acctcgacaa cctgcttgac gatttcgacg gcgttaccgt ggaaggcggc gtcgattcgg
     7801 aaaacgacga cggctgtgaa ggcggagcgt gcaaaatcta actcaggccg tctgaaaacc
     7861 ggcaaacaaa ccacttaaat catcaaaaca ttaaggaaac cacatcatgg catacagcga
     7921 taaagtaatc gaccactacg aaaatccccg caacgtcggt actttcgaca aaaacgacga
     7981 atctgtcggc accggcatgg tcggcgcacc ggcctgcggc gacgtgatgc gcctgcaaat
     8041 caaagtgaac gatgaaggca tcatcgaaga tgcgaaattc aaaacttacg gctgcggttc
     8101 cgccatcgct tcgtccagcc tgattaccga gtgggtcaaa ggcaaaagcc tggatgacgc
     8161 gctggcaatc aaaaacagcg aaatcgccga ggagttggaa ctgccgccgg taaaaatcca
     8221 ctgctccatc ttagcggaag acgcggtaaa agcggccgtt gccgactacc gcaaacgtca
     8281 ggaaaacaga taaagccctt cagacggcat cgtcccgcaa tgccgtccga accacccgcc
     8341 gcttcaggtc cgtccggggc ggtacaacaa ggaagaaata tgattaccct taccgagaat
     8401 gccgcaaaac acatcaatga ctatctcgcc aaacgcggca aaggcttggg cgtacgcttg
     8461 ggtgtgaaaa ccagcggctg ctcggggatg gcgtacaacc ttgaatttgt cgatgaagcc
     8521 gatggcgacg acctgatttt cgaaggacac ggcgcgcgca tttatatcga tccgaaaagc
     8581 ctggtttatc tggacggcac gcaagtcgat tacaccaaag aaggtttgca ggaaggtttc
     8641 aaatttgaaa accccaatgt caaagactcc tgcggctgcg gcgaaagctt ccacgtttaa
     8701 ggcataaaaa cggcgggaca gtatcaaaac cgtcccgcca ttttttcgct tcctgcctgt
     8761 tgtagctgcc tttgcctttc cttttccgtt ccaccttgtg ccggaacaaa tcggatttca
     8821 ctaaggcttt taaagcattg tcgcgtattt tgcctttatt gtgctgcact ttgccgccca
     8881 tattcagtcc tttcgtttaa gaagctgcag attataaggc aaaaacagtt ttctgccaaa
     8941 atcttacatt tatcatccta ctatgtccca atatttcacc ctcttccgga ttgaacccgc
     9001 tttcgatatc gacaccgaaa acttggaaca aacctaccgc gccttggccg cccgtttcca
     9061 tcccgataaa ttcgcttcag cttccgcctt tgagcaaaag caggcagtga tgatgtcttc
     9121 caccatcaac gatgcctacc gcaccttgaa aaaccccatc gaccgcgccg cctacctgct
     9181 gaaaaaatcg ggcatcgatg ccgacgcgcc ggagcatacc gctttcgccc ccgaattcct
     9241 tatgcagcaa atggaatggc gcgaaacgct gatggaggca cgggcaggca acgaccttga
     9301 atccttgaaa aatctcgaca acgaaatccg cgacgagcaa gaaaaactgt tctgcggtct
     9361 gaaacagtcg tttgcccgac aagattacga cacagccgca caacaagtcc gccaaggcag
     9421 gtttctcgac aaactccgca acgaaatttc ctcggcatta taatccgcac cgtgtttcag
     9481 acggcgtaac cgccgcaccg ttccgcgtca aaatatgcta aaataagcaa caattttttg
     9541 ccatacgaaa cattgaaacc atgaccgacg caaccatccg ccacgaccac aaattcgccc
     9601 tcgaaaccct gccggtaagc cttgaagacg aaatgcgcaa aagctatctc gactacgcca
     9661 tgagcgtcat tgtcgggcgc gcgctgccgg acgttcgcga cggtctcaag ccggtacacc
     9721 gccgcgtact gtacgcgatg cacgaattga aaaacaactg gaatgccgcc tacaaaaaat
     9781 cggcgcgcat tgtcggcgac gtcatcggta aataccaccc ccacggcgat accgccgtat
     9841 acgacaccat cgtccgtatg gcgcaaaatt tcgctatgcg ttatgtgctg atagacggac
     9901 agggcaactt cggatcggtg gacgggcttg ccgccgcagc catgcgctac accgaaatcc
     9961 gcatggcgaa aatttcccac gaaatgctgg cagacattga ggaagaaacc gtcaatttcg
    10021 gcccgaacta cgacggtagc gaacacgagc cgcttgtact gccgacccgt ttccccacac
    10081 tgctcgtcaa cggctcgtcc ggcatcgccg tcggcatggc gaccaatatc ccgccgcaca
    10141 acctttccga taccgtcaat gcctgcctgc gcctgctcga tgcacccgac accgaaatcg
    10201 acgaactgat cgacattatc caagcccccg acttcccgac cggggcaacc atctacggct
    10261 tgagcggcgt gcgcgaaggc tataaaacag gccgcggccg cgtcgttatg cgcggtaaga
    10321 cccatatcga acccataggc agaaacggcg aacgcgaagc catcgttatc gacgaaatcc
    10381 cctatcaggt caacaaagcc aagctggtcg agaaaatcgg cgatttggtt cgggaaaaaa
    10441 cactggaagg catttccgag ctccgcgacg aatccgacaa atccggtatg cgcgtcgtta
    10501 tcgagctgaa acgcaacgaa aatgccgaag tcgtcttaaa ccaactctac aaactgactc
    10561 cgctgcaaga cagtttcggc atcaatatgg tggttttggt cgacggacaa ccgcgcctgt
    10621 tgaacctgaa acagattctc tccgaattcc tgcgccaccg ccgcgaagtc gttacccgac
    10681 gtacgctttt ccggctgaag aaggcacgcc atgaagggca tatcgccgaa ggcaaagccg
    10741 tcgcactgtc caatatcgat gaaatcatca agctcatcaa agaatcgccc aacgcagccg
    10801 aggccaaaga caaactgctt gcacgtcctt ggcgcagcag cctcgttgaa gaaatgctga
    10861 cgcgttccgg tctggatttg gaaatgatgc gtccggaagg attggctgca aacatcggct
    10921 tgaaagagca aggttattac ctgagcgaga ttcaggcaga tgctatttta cgcatgagcc
    10981 tgcgaaacct gaccggcctc gatcaagaag aaattgtcga aagctacaaa aacctgatgg
    11041 gtaaaatcat cgactttgtg gatatcctct ccaaacccga acgcattacc caaatcatcc
    11101 gcgacgaact ggaagaaatc aaaaccaact atggcgacga acgccgcagc gaaatcaacc
    11161 cgttcggtgg cgacattgcc gatgaagacc tgattccgca acgcgaaatg gtcgttaccc
    11221 tgacacatgg cggctatatc aaaacccagc cgaccaccga ctatcaggcg cagcgtcgcg
    11281 gcgggcgcgg caaacaggcg gctgccacca aagacgaaga ctttatcgaa accctgtttg
    11341 ttgccaacac gcatgattat ttgatgtgct ttaccaattt gggcaagtgt cattggatta
    11401 aggtttacaa actgcccgaa ggcggacgca acagccgcgg ccgtccgatt aacaacgtca
    11461 tccagttgga agaaggcgaa aaagtcagcg cgattctggc agtacgcgag ttccccgaag
    11521 accaatacgt cttcttcgcc accgcgcagg gactggtgaa aaaagtccaa ctttccgcct
    11581 ttaaaaacgt ccgcgcccaa ggcattaaag ccatcgcgct caaagaaggc gactacctcg
    11641 tcggcgctgc gcaaacaggc ggtgcggacg acatcatgct gttctccaac ttaggtaaag
    11701 ccatccgctt caacgaatac tgggaaaaat ccggcaacga cgaagcggaa gatgccgaca
    11761 tcgaaac
//
LOCUS       DAWHRA010000061        11553 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWHRA010000061 DAWHRA010000000
VERSION     DAWHRA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.061"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            230..442
                     /locus_tag="WIB30_001184"
     CDS             230..442
                     /locus_tag="WIB30_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ3797257.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            590..2173
                     /locus_tag="WIB30_001185"
     CDS             590..2173
                     /locus_tag="WIB30_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ3797258.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESGAFSQTAI
                     NNLEARARLMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDAVKDKIFSICWNNLT
                     KHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTNLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDILLSGIRDVMKTGEAFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2255..3352
                     /locus_tag="WIB30_001186"
     CDS             2255..3352
                     /locus_tag="WIB30_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ3797259.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3466..4581
                     /locus_tag="WIB30_001187"
     CDS             3466..4581
                     /locus_tag="WIB30_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ3797260.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4706..5161
                     /locus_tag="WIB30_001188"
     CDS             4706..5161
                     /locus_tag="WIB30_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ3797261.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5232..5708
                     /locus_tag="WIB30_001189"
     CDS             5232..5708
                     /locus_tag="WIB30_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ3797262.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQQ"
     gene            5753..6046
                     /locus_tag="WIB30_001190"
     CDS             5753..6046
                     /locus_tag="WIB30_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEZ3797263.1"
                     /translation="MKPSLLLQSRKKEILAVFGKYPLICNPRVFGSVSRGDDTENSDI
                     DLLVDAKTGTTLLDLGGLQEELQKLLGIKVDLLTPDDISAHFRDKVLTEAVAL"
     gene            6043..6315
                     /locus_tag="WIB30_001191"
     CDS             6043..6315
                     /locus_tag="WIB30_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEZ3797264.1"
                     /translation="MKMQKELSVYLKQILQAAQYIRLYTDKMDYGQFSADTKTVQAVV
                     FNLFLIGENATHILKSYPEFAEETKYLNWIGMRGYCAIGLPMATLK"
     gene            6498..7415
                     /locus_tag="WIB30_001192"
     CDS             6498..7415
                     /locus_tag="WIB30_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ3797265.1"
                     /translation="MKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEFGEI
                     GIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAHAAS
                     VIDELKTKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTDLCT
                     PEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVIEKSRYRLKALPDNTMGFQS
                     QGFTFPAGRDFDGEKLTNFFNDLPNMTEGLVRAKGVFQVLGTWVWLNWVDGQWGANQV
                     SWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            7480..8037
                     /locus_tag="WIB30_001193"
     CDS             7480..8037
                     /locus_tag="WIB30_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3797266.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TAGTIAMARTTAPDSATSQFFINLADNASLDYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
     gene            8145..10196
                     /locus_tag="WIB30_001194"
                     /pseudo
     CDS             8145..10196
                     /locus_tag="WIB30_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706096.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            10209..>11553
                     /locus_tag="WIB30_001195"
     CDS             10209..>11553
                     /locus_tag="WIB30_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ3797267.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSGGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRVEAARRLKTESDPFYRDYL"
BASE COUNT         3181 a         2763 c         2930 g         2679 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataaat gcgccgattg ccaaggcaat gatgctcaac
       61 cagggttttt gcgaagcgtt cattgttgtt gtcctgatgg gaattgccgc cattatgcgc
      121 aagcgtccgt attttttcaa accgccgcac cgtttccgcg tttgcccaag ttcacgcccc
      181 gacttacaat aacgcccgtc agaatgcagt accaacgaaa ggaaaaatca tggaaaccct
      241 tatcctcgac atcggcggca tgagctgcgg cggctgcgtc aaaagcgtta cccggatatt
      301 ggaaggcgta aaaggcgtgg caagcgtcga agtcagcctt gaaaacaaaa gtgcgaccgt
      361 cggatacgac cccgcgcaaa ccgatgcagg ggcgttgatt gaagccgttg aagacggcgg
      421 ctatgatgcc gcgttgaaat aaagcggcaa aaatgccgtc tgaagccttc gcgcctccca
      481 gatgccgtct gaagtacgct ccgcgccttc agatggcatt tgatttagtt gaacgggtag
      541 gatgaattgg ttaaacttgc gggcaatagt gccttcagac aaacaaaata tgccgaacaa
      601 gaagaaaaaa gccgcctttg cgcgtgctgc aacggagaac ggtgtgccgg acgtgtccaa
      661 catcaaaaat tcatatgatg atttgatgta cgaatccggc gcattctctc aaaccgccat
      721 caataatttg gaggcgcgcg cccgcctgat gggtttgcag cccgcgcccg ccgccaatgc
      781 caaggtcttg gagctgggct gttcgatggg cggaaacatc atcacgcagg cactttatta
      841 cccggatgcg gaatttgtcg gtatcgacct gtccggcagg caggttgcac agggcaacgc
      901 catcattgaa aaaatgggct tgaaaaatgt gcgtctggaa gaaaaagata ttttgaccat
      961 cgatgagtca ttcgggaagt ttgactatat catcgtccac ggcatttggt cgtgggtgcc
     1021 tgacgcagtt aaagacaaaa ttttttcgat ttgctggaac aacctgacca aacacggcat
     1081 tgcctatatt tcatacaatg tttaccccgg ctggaaacgg caggagcagt tgcgcgaaat
     1141 tatgtacttt gccggcaggg atgtgcttga agaacccttg gaagcgcgga cgcggaaagg
     1201 cttggacgcg ctcaaggcgc tggcggaaat tttggaaaac gacaagggct tggacggcgg
     1261 cggcaaactt ccggcgattc aaaaaatatt gaatcataat ttttattaca tcgcacacga
     1321 atatatggag gcgtttaacg acccgattta cgtcaacggc ttcatcgaat gggctaaccg
     1381 ccacaggctc gcatatatcg gggataccaa tttgcacgtg tcttttgttt cttggatggc
     1441 ggagcatacg cgggagcgga ttctggcatt ggcaggggac gattatattg ccaaagaatt
     1501 ttacagtgat attttatccg accgccaatt ccgccgttcg cttttatgcc gtgaggaagt
     1561 cggggatact gtcaggcgtg atgagtcggt tgccgtcgaa gtgatagaaa gtttgaattt
     1621 ccgcccggca agaggggaaa caatcaactt tgatgaaaac gatatcctgc tttcgggcat
     1681 acgcgatgtg atgaaaaccg gagaggcgtt taaaacggaa gatgttgcgg aaaatctcgc
     1741 ccgccgattt cccggtttgg aatttgaccg catgaaaatc aattcccagc ttttattgca
     1801 aaccattctc gggcgttttt ctgtttcatc agacaatgcg ggcaaaccat tttttgaaga
     1861 ccataaaacc tatgtgccgg cgcgctttac aaactatgcc gccgcctttg tggaacacgg
     1921 cgcggaagcg tttgtccggc ctgccaaccg ttacaacgaa agcaccccct cattcggata
     1981 cgggcatttg tacattatgc gccaattgtc gcggccgacg agcaaacagg cattgattga
     2041 aacggttgcc gaaaacttga acatcgtcag caccacgccc gacggtttga cattccatcc
     2101 gcctgccgaa gtgtatgtgg aagaaatatt ggcagacttg gcagacaggc attttctcgt
     2161 ttcggcggat tgacgggcaa gtcccggcgg caatccgtcc gatatatttt cagacaggaa
     2221 agacacacac tgcgaaaagc ggtgcacgac acatatgaac cagacattta ccttgcccga
     2281 tacgcgcccg tatccccaaa atccgattaa aaaccacctg ctgctcaatg cctaccagtt
     2341 ggcgcataat tcttcccagg cttcgcgcaa actctcgtcc ggccaacttc aaaccgaaat
     2401 caggggaatg cttgagcaaa accactatat caacctttcc ctcgcgctga cgatgtcgcc
     2461 cgatgccgga acttatgccg cgctgctttc cagtgtgaac gcggtactcg attgcgagaa
     2521 agaaggcgaa gtgcagtggt tcgccctgcc ggtcgtgctg gtgtccggct gcaaaaaaga
     2581 acgtgcaatc gagatgaagc tgccgacgga ggcattgttt gcctgcctgc aaaactatcc
     2641 gcacctgcgc gcgttgacgc aagagacgca atggctgcct tatcttgtgc attcttccga
     2701 tttgagcgcg gtcgcgccgg atgagtggtg gcgtgccaaa caaaataccg aagcggcggc
     2761 gcaacacttg cgccgtttcg ccccgcgccc tttgctgttg cccgaagggc agtccgtcca
     2821 cgtcgtttac gcgctgggtt tcggcagcgg caaagttcag acggcattgg gtcagaacct
     2881 tcttcaggca ggtctgcccc tgatgcaggt atggcaggaa aatcttgcat cggaaggcgt
     2941 tacgctgttt gccaatccgc tttccccgga ttctccggta cgcgcgcttt cagacggcag
     3001 ccacacgcgc caacgtatgg cgatggatgt gtttgcggca aacgcgatac gcgccgtccg
     3061 tatgcagagt ccgcgcgtcg gcgtggtcgc tgcggcaaag gcgggcggac agattttatt
     3121 cgggtttaat gcgaccgacg gcgcgtttga agtcgtgccg caggtgtttt cgtggcagct
     3181 ttcctttacc gacaacatcg ccgtcatcca gcaaaatttc ctcgacctga tggcggagtg
     3241 ccgcgtggaa cacgtttacc tgttgcacaa tcccttgggc gaacaggaaa gcatcccgag
     3301 ctatgcggaa gcattgaaac gggaagggca caatccgttt ttcagcgcat agtgatttgt
     3361 agtagtgata ggctttctca tttatataat aaatcaaaat aaagagcaca acactttttc
     3421 attctgtgtt gtgccttgag tgaaacgaaa ggatgaatta taaatatgaa gattgtaatt
     3481 gcaccggatt cgtttaagga aagcttgaca gctcaacagg tagctgaagc aataaaaaga
     3541 ggcttccaac aatcgatagc agatgtggaa tgtctcctct gtcctgtcgg tgatggggga
     3601 gaaggcactg tagatgctat ccgacattct cttgacctag aagaaaaatg tctccaagtg
     3661 acaggacctt ttggacaaaa agaagtcatg cgctattttc aaaaagaaca actagcccta
     3721 ttcgaagttg ctgatttggt tggtcttgga aaaatcccgc tagagaaacg aaatccatta
     3781 caaatccaaa ctcgtggtat tggagaattg attcgccacc tcattagtca agaaattaaa
     3841 gaaatctata ttggcgttgg cggtacggcc agtaatgatg gaggtattgg gattgctgct
     3901 ggtttaggtt atcaatttta tgatgaggat ggaaatgcct tacccgtttg cggtcaatcc
     3961 ttactaaacc tagcttctgt ttcaacagaa aatcgctatg aaattcctga agatgttcac
     4021 attcgtattt tagcagatgt tgtgagtccc ttatgtggac accaaggtgc gacctatacg
     4081 tttggcaagc aaaaagggtt ggattctact atgtttgagg ccgtagatca ggcaatacaa
     4141 gatttttatg aaaaagtctc ccctgcaaca ttaaaactta aaggagcagg agctggtgga
     4201 ggcatcgctg gtggtttgtg cgcctttgct caggcaagta tcgtatctgg aattgacacc
     4261 tgcctggact tgattgactt tgataagaaa gtgtcagatg ttgacttggt tatcgttggt
     4321 gaaggaagac tagatcgtca aagtttagca gggaaagcgc ctattggtgt agcaaaaaga
     4381 acccctgtcg gagttcctgt tgtcgctatt tgtggcagtc tcgttgaaga tttgccttcc
     4441 ctgccatttg aaaatatcca agctgccttt tctattttgg agaaaagtga acctttagaa
     4501 gatagtttga aaaatgccag cctctatttg gagcatacgg cttctaatat tggacactta
     4561 ttaaatatgc ctaagattta gccaaaccat ttcttccaga tggatgtttt ggctggttct
     4621 gccttatgcg gttacgatta accgaccgat gccgtctgaa agggtttccg cttcagacgg
     4681 catcgcgccg gaacagggag gggatatgaa aaaaccgaaa atcctttttg tctgcctcgg
     4741 caacatctgc cgttcgccga tggcggaata cattttgcgc cgccgcgccg ccgaagcggg
     4801 cattcccctt gaagcggaca gcgcggggac atcgggctgg cacgacggcg aagatatgca
     4861 ccgcgagacg gcaaagatat tgaaaaaaca cggtatcgat gcttcaggct ttaccagccg
     4921 caaaatccgc caaagcgatg cggcggcgtt tgactgcatc atcgcgatgg acggcaagaa
     4981 tttgtccgaa ttggaaagaa ccttcggcag gcggccggaa aaaatattca agctgaccga
     5041 cctgataccc gaaagcggtt acgaccatgt ccccgatccg tggtatacgg gcgattttga
     5101 agaaaccttc aggcttgcgg atgcgggctg tcgggcattg ttggaaaaga tttccaaata
     5161 aagcaattga atacaaatat aaaaccgcct cctgccgtat cggttgttca gacggcataa
     5221 acagagaatt tatgaaaaca aatttcaaac agaaaattat cgaacaggca cgcagcgaag
     5281 gcttgcaggt aaccgctttg cgcgagcagg tattagatat tgtcttgcag caaagcggcg
     5341 tgattaaagc ctacaacgtc ttgtcgcaga tgcagcagca aagcgagggc gtgcttgcac
     5401 cgcctaccgc ctaccgcgcc cttgattttt gggcggagca gggcgttttg cacaaagtgg
     5461 cggcggtcaa cggctatatt ttgtgcagcc acgcgcagca cgagtgcgac gaccattgcc
     5521 acgaccacga agaagccgaa gcgcaccaca gcgcgtttat tttggtctgc accgaatgtg
     5581 gcacggcgga cgagcaaacc ctgagccacg agtgggcggc actgcgcgca ggcgttgccg
     5641 aaagcggctt tgcgctgaaa gaagaacacg ttgttttaac cgggatttgt aaaaaatgtc
     5701 agcagtaatc ggtggtttgc attgataata agcctttcga aaggagcaga taatgaaacc
     5761 gtctcttttg ttgcaaagca gaaagaaaga aatattggca gttttcggga aatatccatt
     5821 gatttgcaat ccaagggtat tcggttcggt ttctcgcgga gatgatacgg aaaacagcga
     5881 cattgatttg ttggtggatg caaaaacagg gacaacatta ttggatttgg gcgggctgca
     5941 agaggaattg cagaaactct tgggcataaa ggtcgatttg ctgacacccg atgacatctc
     6001 ggcccacttt agagataagg tgttgactga ggctgtcgca ttatgaaaat gcagaaagaa
     6061 ttgtcggtct atttgaaaca gatattgcag gcagcgcaat atatccggct atataccgac
     6121 aaaatggatt atgggcaatt ttctgccgac acaaagactg tgcaggcagt cgtttttaat
     6181 ttgttcctta tcggtgaaaa cgcaacccat atccttaaat cgtatccgga atttgccgag
     6241 gaaaccaaat atttgaattg gatagggatg agggggtatt gcgcaatagg attgcccatg
     6301 gctactttga aatgaacctg tctgtcgttt gggagacggt tttgaacgtc ataccggcga
     6361 tgtcagccga tatatcgaat ttgctggaac aattgtcaat tgatgaagaa catgataaca
     6421 gctgccatca cttttaacat tatttaatca tttaaaacac cctatcttca aattttaaag
     6481 tgggacatca catagaaatg aaaaaaacca aagtccacct gatttcaggt tttctgggaa
     6541 caggcaaaac caccgcgctc aaaagcctga tggcgcaaaa agacccgaac gaaaaatggg
     6601 tcatcatcgt caacgagttc ggcgaaatcg gcattgacgg cgcggtattg agcgacaacg
     6661 gcatccctgt ggcagaaatc gccggcggct gtttgtgttg caccgccggc ccgcaaatgg
     6721 gcgtaaccgt gcagaaaatg ctgcgcgacg ccaagcccga ccgcctgatg attgaagcaa
     6781 gcggactggc gcacgccgcc agtgtcattg atgaactgaa aaccaaaccg ctggacagcc
     6841 ttttggaaat cggcgcagtc tttaccgtcg tcgatccgcg ccagttcatc aaccccgatt
     6901 acgcgcagca ggcattgtat aaagaccaaa tcggcatctg cgacgtattg gttgccagca
     6961 aaaccgattt atgcaccccc gaacagcttg ccgaatttca cgacaaagcc gcaaaactgt
     7021 tcccgcccaa ggctaaagtg gtcgaagttc aaaacgcaca actcgatatc caatggcttg
     7081 acatccccgt catcgaaaaa tcacgctacc gcctcaaagc cctgccggac aacacgatgg
     7141 gcttccagtc gcaaggtttc acattccccg ccggacgcga tttcgacggt gaaaaattga
     7201 ccaacttctt caatgatttg cccaatatga ccgaaggact cgtccgtgcc aaaggcgtgt
     7261 tccaagtatt gggaacgtgg gtgtggctca actgggtgga cgggcaatgg ggtgcgaacc
     7321 aagtgtcttg gcggcgcgac tcgcgttttg aattgattgc caaatcgttt gatgcggatt
     7381 taatcgaaca aaaactcaaa gacgcattgg aatagcgtta acgagccttc agacggcata
     7441 ggaaatgaaa atgccgtctg aaggtctgag gaggacggga tgaaacccaa attcaaaacc
     7501 gttttaaccg cgctgctttt ggcggtttcc ctgccgtcta tggcggcaac ccatgttttg
     7561 atggaaaccg atatgggcaa tatccgtttg gttttggacg aatccaaagc ccccaaaacc
     7621 gttgctaatt tcgtgcgcta tgcccgaaaa ggcttttacg acgacaccgt ttttcaccgc
     7681 gttatcgacg gttttgttat ccagggcggt ggattgaccg aggacttggc acaaaaggca
     7741 agcgataagg ccgttgccaa cgaatccggc aacggcttga aaaacaccgc cggcaccatc
     7801 gccatggcgc ggacgacagc ccccgattcc gccaccagcc aattctttat caatctggcg
     7861 gacaacgctt cgctcgacta caaaaacgga caatacggct ataccgtttt cggcagggtc
     7921 gaaagcggca tgaacaccgt ttccaaaatc gcccgcgtca aaaccgccac gcgcggcttt
     7981 tatcaaaacg tacccgtaca gcccgtcaaa atccgtcgcg ttgttgtcgg gcagtaacac
     8041 gcagacagac gttcagacgg cgtcgcccgt ttcccaaaaa acgccgttta aagtaaaaaa
     8101 atattttaaa acagacagtt gatattgaca aattcaaacc gaggatttta aaatgctgcc
     8161 aacccaaccc aacccaaccc aacccaaccc aacccaaccc aagccctgat ctaagcgaca
     8221 cagccggggc gaacactgag gcaatctaca cttcagacgg cattaccgcc aactctaccc
     8281 aactcgaaca gctcaaaaaa ctgtttcccg cctgttttga cgcagacgga aatttcctta
     8341 tcgacagatt acaagccgaa atcgcgccgc agaccgacat cggacgcgaa ttttacgaaa
     8401 tgaactggct gggcaaatca tatgctcgcc tgcttcgcaa cctgccgccc gaaaccctga
     8461 taagtgaaga caagacgcac aacgccaagc ccgaaaatgc aggcagccaa aacctgctga
     8521 ttcgtggcga caatctagaa gtgttgaaac acttaaaaaa cgcctacaca aacagcgtga
     8581 agatgattta catcgacccg ccctaaaaca ccggatcaga cggctttgtc tatcaggacg
     8641 accgcaaatt cacacccgct gaacttgccc gcctagccaa tattgatgaa gacgaagccg
     8701 cgcggatttt agatttcacc gacaaaggct caaactcgca cagcgcatgg ctcaccttta
     8761 tgtatccgcg cctgtatgtc gcccgcgaac tgttaaagga cgacggtgtg atttttatct
     8821 cgattgacga taacgaagcg gcgcagttga aattgttgtg tgatgaagtg tttggggaag
     8881 ggaattttgt tgcacaattg ccttggcgaa aaagaacagc taaatcagat gtgccttttg
     8941 gtatttcgca ggattatgaa tggatatttg tattcgcaaa atcttgccaa tttattgcag
     9001 caactaaagg caaggaacga cgctattatg agactgatga tttccccgat cgtccttggc
     9061 gtacccacga cttaacgaaa caaacaactg cggcggaaag gccaaatagt tttttcacaa
     9121 tggttgatcc caagacagga aaaaaatatc cagcaaatcc aaatgcaact tggcgtgtaa
     9181 ccaaagatac atttcaagat tattataata aaggaaaaat cgttttccct gatgattatg
     9241 atttcttaaa tatcagcaat ccagttatgc gttactttaa agatgacgac atgaaaaaag
     9301 ctggcgagga ttttggcaaa gtagctgtaa gcagtagatt acctgaaaat gttggtacat
     9361 tagccgatgc agtagccgaa tatttggcta tttttagtag gacgctacct gaaaatatcg
     9421 gaatgactaa agaaggcaca aaagaaatca cagatttatt tggtagcaaa atatttacct
     9481 tccccaagcc tagtcaattg attaaatttt tagtttcaat aagttcaaag agtaatgacc
     9541 taatcctaga cttcttcgca ggcagcggca caaccgccca cgccgtgatg cagcttaacg
     9601 ccgaaggaca aaacggtaac cgccgctata tctgtgtaca gcttcccgaa aaaaccgctg
     9661 aaaaatccga agcccgtaaa gcaggctacc cgaccatctt cgacatcacc aaagcccgca
     9721 tagaaaaagc cgccgccaaa atccgcgtcg aacatcccga ttacacgggc gattcgggct
     9781 tcaaaatctt tcaaacggca gacaatttca ggcagcatcc ggacaaggat ttttcgcccg
     9841 aacaaccgga tttgccgctt aacgatgaat taagcgaaga acagctgcaa acgcttctga
     9901 ccacctggac gctgtatgac ggggcggcac tgaccacgcc ggttgagcct gtgcggttag
     9961 gggcttacac ggcgtatctg tgcgaaaaac ggctgtatct gatgaatgcc ggttttactt
    10021 ccgccgattt gttggcgttt atccgcaagc tggacgacga tgcggatttc aatcccaacc
    10081 gcgtgattgt attcggcagc aatatggcaa gcgccatgca gcacgaactt gaccaggcgg
    10141 ttcgcggtta tgccaataaa aaagagattg agttgaatgt ggttatcagg gtttgacgga
    10201 ggcaattcat gagcggtttt aattacgaga aaaaccagcc gcaccaaatg cgggcggttt
    10261 cggcggtttt gggcgtgttt gacggggcaa cgcccaaata tcggacggca gacgaaaatc
    10321 ccgaactttt gtttgctgca aaacaatacg caaacaatat cctgaaagtg caaagccaaa
    10381 acggtataga cggccgattc cccgaccgtt cggacgacca aaatatcctt gatatttcca
    10441 tggaaacggg cacgggcaaa acctatacct acacacaaac catgttcgag ctgcaccgtt
    10501 ggctgggcgt gttcaaattt atcgtggtcg tgccgacttt gtccattaag gcgggaacac
    10561 agcagttttt gcaaagcaag gctttggcag agcattttga acaggatttc ggcggcgatt
    10621 atgaaggcgt gcgcctgaaa acctatgtgg tggaaagcgc gaaaaagaat aagggcaaaa
    10681 agtccaatgc gcccataacg attgagcaat ttgtcaaagc ggaaaacaaa aaggaaatcc
    10741 atgtgctgct gattaacgcg ggcatggtta attcgtcgtc catgaacgat acgggcgaca
    10801 aggcattgaa agatttgttt gacaatcccg ttgatgcatt ggccgccgtg cgcccgtttg
    10861 tgattgtgga cgaaccgcat aaattcccga cccgggagag cgcgaaaacg tggggcaata
    10921 tcaaacgctt aaaaccgcaa tatattttgc gctacggtgc gacatttaac gatgaatatt
    10981 acaacttgct ttaccgtttg acggcagtag acgcgtttaa cgacgggctg gtcaaaggcg
    11041 tgcgcgtgtt tcaggaagaa atgcagggcg gcatggatgc ggcggtaaaa ctggtgtcgt
    11101 cgggcggcaa agaagcgaaa tttgaattaa acgaaaagga caaaaagcag acgttcaaac
    11161 tggcaaaagg cgaagatttg gcgcaaatcc atccggctat ttcggatttg aaaatcgaca
    11221 aaatgaataa aaccgtggtg gtgttaagca acggcttgga gttgaaaacg ggtgccgtca
    11281 tcaaccctta ttcctattcg caaacggtgc aggatgcgat gatgcagcgg gcggttgccg
    11341 aacatttcaa gctggaacgc gcgcttttgg cagaacgcgc gccacagccc aaaatcaagc
    11401 cgctgacgct gttttttatt gacgatatcg cgggctaccg cagcggcaac gagctttcag
    11461 gcagcctgaa agataaattt gaaagctgga ttcgcgtgga agcggcacgc cgtctgaaaa
    11521 cggaaagcga cccgttttac cgcgattacc tgc
//
LOCUS       DAWHRA010000062        11544 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWHRA010000062 DAWHRA010000000
VERSION     DAWHRA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.062"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            499..1800
                     /gene="bioA"
                     /locus_tag="WIB30_001196"
     CDS             499..1800
                     /gene="bioA"
                     /locus_tag="WIB30_001196"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ3797268.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKCRLKTAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1797..2444
                     /gene="bioD"
                     /locus_tag="WIB30_001197"
     CDS             1797..2444
                     /gene="bioD"
                     /locus_tag="WIB30_001197"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ3797269.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2411..2641
                     /locus_tag="WIB30_001198"
     CDS             2411..2641
                     /locus_tag="WIB30_001198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797270.1"
                     /translation="MDGVGKNRRGIKIGKNMEHLYDEYGNTTPNRRTTNKKDETISSK
                     VTVAAIPVAAPFAIADIVDQDFIQAIMKITGH"
     gene            2665..3051
                     /locus_tag="WIB30_001199"
     CDS             2665..3051
                     /locus_tag="WIB30_001199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797271.1"
                     /translation="MKCYIKVVIIVFIMVMTTSCNPCFFATSSCDYSFRKNYPINSDD
                     EYYVKNNTSKETRIADLRYCISYACPEVYQKYQLPDENGDYREIIRRKYKDDYKKGYK
                     NADDVMTCYGKIGECMKNNKGYSTKW"
     gene            3045..3488
                     /locus_tag="WIB30_001200"
     CDS             3045..3488
                     /locus_tag="WIB30_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797272.1"
                     /translation="MVMFRLLSTALVLLIMQGCYYTTIDNEKCFVAPQSVHCPVSSDI
                     ENVIRKNPNKSINQVRSDIKYCANMYFSNIRELNIPQDFDNYIFMLKEKGIPRNDKEL
                     AYNAVRNMHIICLSSLGGYDYYQKTAEFQKIDYKKEMEKLLNENH"
     gene            3670..3942
                     /locus_tag="WIB30_001201"
     CDS             3670..3942
                     /locus_tag="WIB30_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797273.1"
                     /translation="MTIRANNDIEYFVKNNTTNEQRIDDFRSCLSVACPEIYKLYQLP
                     DEKGNYQYIIKKYYDNKYNDNAVKSTNCIGDNARCMLNNKGYSTKW"
     gene            3945..4421
                     /locus_tag="WIB30_001202"
     CDS             3945..4421
                     /locus_tag="WIB30_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ3797274.1"
                     /translation="MGYGFEEWLPDLPAPVSDGIGLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            4451..5341
                     /gene="ubiA"
                     /locus_tag="WIB30_001203"
     CDS             4451..5341
                     /gene="ubiA"
                     /locus_tag="WIB30_001203"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ3797275.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            5526..5975
                     /gene="ptsN"
                     /locus_tag="WIB30_001204"
     CDS             5526..5975
                     /gene="ptsN"
                     /locus_tag="WIB30_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ3797276.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            5977..6939
                     /locus_tag="WIB30_001205"
     CDS             5977..6939
                     /locus_tag="WIB30_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ3797277.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNENRPD"
     gene            6920..7774
                     /gene="rapZ"
                     /locus_tag="WIB30_001206"
     CDS             6920..7774
                     /gene="rapZ"
                     /locus_tag="WIB30_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ3797278.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            7855..8796
                     /gene="ylqF"
                     /locus_tag="WIB30_001207"
     CDS             7855..8796
                     /gene="ylqF"
                     /locus_tag="WIB30_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ3797279.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            8897..9499
                     /locus_tag="WIB30_001208"
     CDS             8897..9499
                     /locus_tag="WIB30_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ3797280.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            9683..11356
                     /gene="recN"
                     /locus_tag="WIB30_001209"
     CDS             9683..11356
                     /gene="recN"
                     /locus_tag="WIB30_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ3797281.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPTLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRSLGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDKIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            11466..11542
                     /locus_tag="WIB30_001210"
     tRNA            11466..11542
                     /locus_tag="WIB30_001210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11500..11502,aa:Arg,seq:acg)
BASE COUNT         2986 a         2714 c         3008 g         2836 t
ORIGIN      
        1 ggctgtaacc ttcaatcgga atttgttgcc tgcgggatat gcggtatgaa tgtttgggat
       61 atatggggca ggatggtgga aatctatcat aagtataaga agccgtgcct ggttttggcg
      121 gtggattttg tgatgggtat ggtattcata gagccgaatg aggagctgtg catcggtaga
      181 tgctatgcgc ctatgtcgga gtcccctgat tttgctaacg ctgttgcgat ggctgttgct
      241 atgatctgta tcgtatggat tgccgttttg tctatgactg attgaagcgt tgggcggagg
      301 ctgtgtgaaa caatattggg cgttgggtcg tctgattcca atcgggcttg gggaatgcga
      361 aacggtgtgc gcttatactg cggacgattt gttgcgtggt tttacgcccg aaacggatgg
      421 aaaggtgtgg gaagcggtct gtcggagtag aatacgcgtt ttgcgtttga atgcagcaat
      481 aagaaaagag agaaacttat gccgtctgaa catcaacaca catcatcatt gcttgatttc
      541 gaccgtaccc atctgctcca tccctatact tccatgaccg atccgcttcc cgtttatcct
      601 gtcaaacgtg cagaaggggt gtttatcgaa ttggcggacg gcacgcggct gattgacggg
      661 atgtcgtctt ggtggtgtgc gatacacggt tacaatcatc ccgttttgaa tcaggcggtt
      721 gagacgcaga tgaaacagat gtcgcatgtg atgttcggcg gtttgacgca tgagccggcg
      781 gtggagctgg gcaaattgtt ggtcgggatt ttgccgcagg ggctggatcg tattttttat
      841 gcggattcgg gttcggtttc ggtggaagtc gcgttgaaga tggcggtgca ataccagcag
      901 gcgcggggtt tgcgtgcaaa acaaaatatg gcgacggtgc ggcgcgggta tcacggcgat
      961 acttggaacg cgatgtccgt ctgcgatccg gaaacgggga tgcaccatat tttcggcagc
     1021 gcgttgccgc agcgttattt tgtcgataac ctgacaagcc gtttcgacga tgaatgggac
     1081 gaggcggatt tgcagcctgt ccgcgccttg tttgaagcgc atcatacgga cattgccgcc
     1141 tttattttgg aaccggtcgt gcagggcgcg ggcggtatgt atttttatca tccgcagtat
     1201 cttcgcggat tgcgcgattt gtgcgacgag tttgatatcg ttttgatttt tgatgaaatc
     1261 gccaccggat tcgggcgcac gggcaagatg tttgcctgcg aacacgcgga ggtcgtgccg
     1321 gatattatgt gcatcggcaa gggtttgagc ggcggctata tgacgctggc ggcagcaatc
     1381 acttcgcaaa aagttaccga aacgatttcg cgcggcgaag cgggcgtgtt catgcacggc
     1441 ccgacattta tggcgaaccc gctggcgtgt gccgttgcct gcgcttcggt caaactgctt
     1501 ttgtcccaag attggcaggc aaatatccgc cgcatcgaaa gcatcttaaa atgccgtctg
     1561 aaaaccgcgt gggacattcg tggtgtgaaa gacgtgcgcg ttttgggcgc catcggcgtg
     1621 atcgagttgg aaaaaggcgt ggatatggcg cgttttcaag cggattgcgt ggcgcagggc
     1681 atttgggtgc gcccgttcgg caggttggtg tatctgatgc cgccttacat catttcagac
     1741 ggcgttttga ccaaacttgc cgacaaaacc gtgcaaatct tgaaggaaca cagcaaatga
     1801 aaggcgttta cttcgtcagc ggcatagaca cggacatcgg caaaaccgtc gccaccggcg
     1861 tgttggcaaa acaattgttg cagcagggca aaagcgtgat tacgcaaaag cccgtgcaaa
     1921 ccggttgcca aaacattgcc gacgacatcg ccgtccatcg taaaatcatg ggtaaaccca
     1981 tgcaggaagc cgacaaacaa gggctgacca tgcctgaaat cttcagttat ccagcttcgc
     2041 ctcatctcgc tgcccgtctt gacggcaggg ctttggactt ggacaaaatc cgcaccgcca
     2101 cacaagaatt ggcggcgcag tacgaagtcg ttttggtcga aggcgcgggc ggattgatgg
     2161 ttccgctgac ggaaaacctg ttaaccattg attatatccg tcagcaaggc tatcccgtca
     2221 tcctcgttac cagcggacgg cttggcagta tcaaccacac tttactcagt ttcgccgcgc
     2281 tcaaacaata cggcattcgc ttgtacagcc tcgtgttcaa ccacatccac gacagccgcg
     2341 acgcacacat cgcccaagac agcctgagct acttgaaatg ccgtctgaaa gccgattttt
     2401 ccgaagcgga atggatggag ttggcaaaaa cagacgcggt ataaagattg ggaaaaatat
     2461 ggaacaccta tatgatgaat atggaaatac aacgccaaat agaagaacaa caaataaaaa
     2521 agatgaaact atttcaagta aagtaactgt tgcagcgata ccagtagccg ctccatttgc
     2581 tattgctgat atagtcgatc aggattttat ccaagcgatt atgaaaatta ctggtcattg
     2641 attatctata tattggtggt tgttatgaaa tgctatatta aagttgtcat tattgttttt
     2701 ataatggtaa tgacaacttc ttgtaatcct tgcttttttg caacttcttc ttgtgattat
     2761 tcttttagaa aaaactatcc tataaatagc gacgatgaat attatgttaa gaataatacg
     2821 agcaaagaaa caagaattgc ggatttacgc tattgtattt cttatgcatg tccagaagtt
     2881 tatcaaaaat atcaactacc agatgaaaat ggagattata gggagattat tcgtagaaaa
     2941 tataaagatg attataaaaa gggctataag aatgcagatg atgtcatgac atgctacgga
     3001 aagattggag aatgcatgaa aaataataaa ggatattcaa caaaatggta atgttccgct
     3061 tattatcaac agcccttgta ttgttgataa tgcagggctg ttattacaca actattgata
     3121 atgaaaaatg ctttgttgcc ccacaaagcg ttcattgtcc tgtaagttct gatattgaaa
     3181 atgttattag aaaaaatcct aataaaagca taaatcaagt taggagtgat attaaatatt
     3241 gtgctaatat gtatttttcc aatatacggg aattaaatat tccgcaagat tttgataatt
     3301 acattttcat gcttaaagaa aaaggtattc caagaaatga taaggaattg gcttataatg
     3361 cagttagaaa tatgcacatt atttgtttga gttctttggg tggttatgat tattatcaaa
     3421 aaaccgcaga atttcagaaa atagattata aaaaagaaat ggagaaattg ttaaatgaaa
     3481 accattaaat ttgctattgc cgatatagtt gatagcgact ttatccaagt gattatgaaa
     3541 ttaagcgggc attgatttat gattacgcgt tgttttaaag ttgtcattat tgtttttata
     3601 atggtaatga caacttcttg taatccttgc ttttttgcaa cttcgtcttg taattatcct
     3661 tttgatgata tgacgataag agcaaacaac gatattgaat attttgttaa aaataatacg
     3721 actaacgaac aacgaattga tgatttcaga agttgccttt ccgttgcttg tcctgaaata
     3781 tataaattat atcaattacc tgatgaaaag ggtaattatc aatatattat aaaaaaatat
     3841 tatgataata aatacaatga taatgcagtc aaatcgacaa attgtattgg ggataatgca
     3901 agatgtatgc ttaataataa aggatattca acaaaatggt aataatgggt tacggctttg
     3961 aggaatggct gcccgacctg cccgcccctg tttcagacgg catcggcctg ccgatgagcc
     4021 ggttattgaa agcccggtcg ctgaccgccg cattgcgcgc cttgccgcat acattttcgg
     4081 tggaactgct gaaactgggc gaattggaga cggaatgcgg agggaggctg gtgcgcgaag
     4141 ttttgttgaa gctggacggt atccctgttg tcgcggcaag gagcgagtgc cgtatcggtt
     4201 cggcgttttg gcaaaacatt ttggactgcg gcacgcgtcc tttgggcgag cgtctgtttc
     4261 aagccgattt ggaaggggcg cgttcggcgt ttgagtttgc cgtttccggc gaaggatgcg
     4321 gacggtactt tgccgcgcgg cgttctcggt tttcccgtca cggcgaggaa atgctgctga
     4381 ccgagtattt tctgcccgaa ctgaaacgct ttatcggata aaataccgtt ttttcaagct
     4441 gtgcggcaat atgaatccta aatccccttt atttttacga ctgtccgatc gtttggatgt
     4501 gtacctgcgc ctgatgcggg cggacaagcc cattgggacg ctgcttttgc tgtggccgac
     4561 ctactgggca ttgtggctgg cttcagacgg cattcccgat ttggcggtat tggcggcgtt
     4621 tacaatcggc acgtttttaa tgcggagtgc cggttgcgtc atcaacgatt ttgccgaccg
     4681 cgattttgac ggtgctgtcg agcgcaccaa aaaccgtccg ttcgcacagg gtcgggtcgg
     4741 aaaaaaagaa gcgctgctgc tgacggcatt tttgtgcctg cttgccgcat tgtgcctgat
     4801 tccgctgaat catctgacat ggctgatgag cctgcccgcg ctgtttcttg cgctgactta
     4861 cccgtttacc aaacgctttt ttccgattcc ccaactctat ctcgggcttg ccttttcctt
     4921 cggtatcccg atggcgtttg ccgccgttgc cggaaacgtg ccgcctcaag cgtggatact
     4981 ctttgccgcc aatgtgttat ggactctggc gtatgacacg gtttatgcaa tggcggacaa
     5041 agaagacgat ttgaaaatcg gcatcaaaac ctccgccgtc acgttcgggc gttacgacat
     5101 cgccgccgtt atgctgtgtc atggcggctt taccctgctg atggcagtat tgggtgcggt
     5161 tatcggtgcg gcatgggcat attggacggc aatccccatc gtcctgctgc tgcaataccg
     5221 ccaatatgcc gccatcaaaa gccgcgtccg gcaaatctgt tttgaaacgt ttttggcaaa
     5281 caacagaatt ggttgggtgt ggtttaccgc catttttgcc catacgtttt tcgcgaaata
     5341 aggcagggca atgccgtctg aagagccgta aactgctttg gacggcattt ctatctgtgc
     5401 cgaaaagcgt taaaatatgt ttttaaaacg ctgtgttatg tcagcccgta ccgtatgcgg
     5461 gattgagatt tgccctagca gtcggtacaa tctttctgtt ttgcgatgtc tgaaaagaga
     5521 agcttatgag ccttatcggc gaaattttgc ctttgtccca tattgttttg gatatggagg
     5581 tgggcagtaa aaaaaggctg tttgaggaag cgggcctgct tttggaacgc gaatcctcat
     5641 tgtcccatgc tgatgttttc gaatgtcttt ttgcccgtga aaaactcggt tcgaccggtt
     5701 tggggcaggg cgttgccatc ccgcacggcc gtcatgccgg cgtgaagcag gcgacgggcg
     5761 cgttcatccg cacgcgcgaa cccgtcggat ttgacgcacc ggacggcaag ccggtttccc
     5821 tgatttttat cttgctggtg ccggaaaacg caactggcga acatttggaa gtcttatcca
     5881 aactggccgg caagttttcc caaaaaagca tccgcgaatc gctgatgacg gtttcttctg
     5941 cggaagaagc ccgcgccatt ttgactgaag aataatatgc ccagtatttc cgtccgccga
     6001 ctgtttgacg acaaccaata caagttacaa cttgcctggg ccgccggcaa ttcgggtgcg
     6061 gacaaccgta tcggcgtaga ggcggacaag cccgtcctcg ctttggtcgg acacctgaat
     6121 ttcatccacc ccaaccaaat ccaagtggtc ggtttggcgg aatcggaata tctgaaccgt
     6181 ctcgaatcgg gggaaacggg ttatcagttc ggcgacctgt tcgacatttc tatgtctttg
     6241 gttattgtgg caaacggctt gccggtttcc ccggggctgc gcgactattg ccataaaaac
     6301 gatattccac tgctgacttc caaactcgaa agcccctatc tgatggacgt gttgcggatt
     6361 tacctgcaac gcaccctggc ggcatcgtcc gtcaaacacg gcgtatttct cgatgtgttt
     6421 gaaatcggcg tgctgattac cggacattcc ggcttgggca agagcgaatt ggcgttggaa
     6481 ctgatttcgc gcggccacag cctgattgcc gacgatgcgg tcgagctgtt ccgcatcggt
     6541 ccggaaacat tggaagggcg ttgttcgcct atgctgcgtg attttttgga agtgcgcggc
     6601 ttggggatac tcaatatccg ccatattttc ggcgaaactt ccatccgccc caaaaaaatc
     6661 ctgcaactca ttatcaattt ggtcgaggca gacgacgagt atatgaagca gctcgaccgg
     6721 ttgagtatcc gcaccgaaac cgaatccatc ctcaacgtca acgtccgttc ggttacgctg
     6781 cccgtcgccg tcggacgcaa cctcgccgtt ttggttgagg cggcggtacg caattatatt
     6841 cttcagttgc gcggtaagga tagtacgcgc gaatttttgg aacgtcatca gacgcagctt
     6901 aaagaaaacg agcaacacaa tgaaaatcgt cctgattagc gggttgtccg gttcgggcaa
     6961 atccgtcgca ctgcgccaaa tggaagattc gggttatttc tgcgtggaca atttgccttt
     7021 ggaaatgttg cccgcgctgg tgtcgtatca tatcgagcgt gcggacgaaa ccgaattggc
     7081 ggtcagcgtc gatgtgcgtt ccggcataga catcggacag gcgcgggaac agattgcctc
     7141 tctgcgcgga ctggggcaca gggttgaagt tttgtttgtc gaggcggaag aaagcgtgtt
     7201 ggtccgccgg ttttccgaaa ccaggcgagg ccaccctctg agtaatcagg atatgacctt
     7261 gttggaaagc ttaaagaaag aacgggaatg gctgttcccg cttaaagaaa tcgcctattg
     7321 tatcgacact tccaagatga atgcccaaca gctccgccat gcagtccggc agtggctgaa
     7381 ggtcgaacgt accgggctgc tggtgatttt ggagtccttc gggttcaaat acggtgtgcc
     7441 gaacaacgcg gattttatgt tcgatatgcg cagtctgccc aacccgtatt acgatcccga
     7501 gttgaggcct tacaccggta tggacaagcc cgtttgggat tatttggacg gacagccgct
     7561 tgtgcaggaa atggttgacg acatcgaaag gtttgttacg cattggttac cgcgtttgga
     7621 ggatgaaagc aggagctacg ttaccgtcgc catcggttgc acgggaggac agcaccgttc
     7681 ggtctatatt gtcgaaaaac tcgcccgaag gttgaaaggg cgttatgaat tgctgatacg
     7741 gcacagacag gcgcaaaacc tgtcagaccg ctaattccgt caaaccatta tgccgtctga
     7801 aaccccgtcc tccgtttcag acggcatttc aaatattgaa aagaaagaac agacatggca
     7861 atccaatggt ttcccggcca tatgaacaag gcgaaaaaag ccatcgccga gcgtgcaaaa
     7921 agcgttgata tggtgattga aatgctggac gcgcgtatgc ccgcctccag cgaaaacccc
     7981 ctgcttgccc agctttccaa aggtaaaccc aaacttaaaa tcctcaacaa acaagatctt
     8041 gccgaccccg agcgcaccaa aatctggctc gaacactata acagccgccc cgacacctgc
     8101 gccatcgccc tcgattcctc cgaaacaggc gcacacggca aaattaccca agcctgtcgt
     8161 gccatgattc cccaccggca aggcatagac aaacccttgc gcgtcttgat ttgcggcatt
     8221 cccaacgtcg gcaaatccac cctcatcaac ggcatgatag gcaaaaaatc cgccaaaacc
     8281 ggcaacgaac ccggcatcac caaagccgaa caacgccttt tccttgccga cgacttctgg
     8341 ctgtacgaca cccccggaat gctgtggccg aaaatcattg tcgaagaaag cggctacaac
     8401 ctcgccgccg gcggttcagt cggacgcaac gcattggacg aagaagaagt cgccctcgaa
     8461 cttttagact acctccgccg ccactacctc cctatgctgc aagaacgcta ccaagccgac
     8521 aaagacccca gcagccactg ggacgaaaac gtttggctcg aatggatagc caaaaaacgc
     8581 ggcgcagtcc tcagcggcgg acggatcaac taccaaaaag ccgccgaaaa catcctcacc
     8641 gacttccgtg aaggcaaaat cggcagaatc accctcgaaa cgccgaacca atgggaaact
     8701 tggctcaaaa aagcccgtca gaaagaagcc gaactcaaag ccatacgcga agccagaaaa
     8761 gcagagcgta aagggcagaa gccttcggaa gcataaagaa tgccgtctga aaaatatttt
     8821 tcaggcagct tctctctact ccaaccgatt tcagacggca tatccaaacc catgccgttt
     8881 cagcacggat acccgtatga ccgacaaaat ttctcccgac gcgctgattg aagccgcact
     8941 gctgacccaa accgaaccgc tgaccgaaaa atctatgcgc gaattgtgtg tgccgccact
     9001 gtcgcaggac aaactgattg atgtgttggc gcagttgaaa acgcgttggc aggatagggc
     9061 gttgcaactg gtgcatacgc aagagggctg gcgttttcag attgttcaga cggcattcga
     9121 gcggctgggc agcctgcaag aacagcgtgc gccgcgctac tcccgcgccg tgatggaaac
     9181 actggcgatt atcgcctacc agcagcccgt aacgcgcggc gacatcgagg gcatacgcgg
     9241 cgtggcggtg tcgcagaacg tgatgcagac cttgcaggat cgggggtgga ttgaagtcat
     9301 cggacatcgg gacacattgg gaaaacccgc attgtgggca acaacggcaa cgttcctcag
     9361 cgatttgcgc ttggacggtt tggaagaact tcctccgctg accgaactgg gcgaactggt
     9421 tttgcccgat ttaatggaaa tgccgcctac ggatgaagaa gagccggaag ccgtaccgtc
     9481 cgataccctg cccaactgaa attccaaatg ccgtctgaaa cgcacattgc ttcagacggc
     9541 attgcaacaa ataagcagat aaaaacaagc attaagaaaa attaaggaaa aacttatttt
     9601 taatttaaaa aatcttagtt ataattcgta tatctaaagt tgatattgct tttgtcggta
     9661 gaattgctaa ggaatcctca cgatgcttct aacactttct ttgcgtgatt ttgtcattgt
     9721 tgaaaatctg aatctggatt ttcaaagcgg ctttaccgta ttgaccggag aaactggcgc
     9781 gggcaagtcc attactttgg atgcgattgg tctgctgttg ggcgataaag ccgattacag
     9841 ccaagtccgc agcggcgcaa aagaagcgca gttgtcggca ttgttcgata tttcccattt
     9901 gcctacttta aaagcagaat tgtatgaaca ggggctttta aacgacggag aagaagaact
     9961 cagtatccgc cgcattatcg atgccaaagg caaaagccgc agctttatca acaatcaggc
    10021 cgctaccttg gcgcaactca aagccgtcgg cagccagctt atcgacatcc acgggcaaaa
    10081 cgcccatcat tcgcttaatc aggaagccgc ccagcgcgaa ttgttggacg catttgcggg
    10141 cagcagggcg caggcggaaa ccgtcaggca gctttatcaa aactgggcaa actcaaaaaa
    10201 agcattgcag gaagcgcagg aacaggcaga aaacattgca ctcgaacaag agcgtctgga
    10261 atggcagttt aacgaattga atcagttgga cattaaacaa ggcgaatggg aagctctcag
    10321 ccaaagccac gacagccttg cccattctgc cgagctgttg caggctgccg aagaagtcgg
    10381 aagcaagatt gacggcgaca acggcatcca acgccatatc tatcaatgtc aaaaactatt
    10441 ggccaatctg caaaacatcg agccgcgctt tgccgagagc ctgaatatgt tggcaagcat
    10501 cgaagccgaa ttgggcgaaa tcagtgccaa tatgcgcgat gtggcaggac gcagcgacat
    10561 caaccccaat gaattggcgg cacaggaaca gcgtatgggc gaactgatgg ggatggcgcg
    10621 gaaataccgg atcgagcctg aagagttgcc tgccaagttg gcagaaatcg aagaacgcct
    10681 gcaaagcctg caagctgccg ccgatttgga cgcgctcgag cataatgttg cccacaattt
    10741 tgccgaatat caggaagctg cccacatcct ttctgccatg cgccatcagg cggcagagcg
    10801 tttgagcggc gaaacgaccg agcatatgca acaccttgcc atgaaaggtg cacgtttcga
    10861 catcgtcctg ttgccctcgt cgccgacggc acacggtttg gagcaggttc aatttcaagt
    10921 tgccgccaac aaaggcaatc cgccccgtcc gctgaataaa gttgcctccg gcggcgaatt
    10981 ggcgcgtatc agccttgcct tacaggttgt tgccagccaa tatacccaag ttcccaccct
    11041 gatttttgat gaggtcgata ccggtattgg agggggagtg gctgaaatgg tcggcaaggc
    11101 attacgttcg ttgggcagaa aacatcaggt gcttgccgtt acccatcttc cgcaagtcgc
    11161 atcctgcggc gaaaaccact ggcgggtgcg caagcacagc gagggagagc aaaccgtcag
    11221 cgaaatcagt atattggata aaatccaacg ggtcgaagag gttgcccgta tgttgggcgg
    11281 agaagtcatt accgatacga cgcggcaaca tgcggcagaa ttgctgcaac ttgcgtcgaa
    11341 aaatagttta ttttaaaatc aatcagttaa aaaataacta aaaataaaag tctaaaacaa
    11401 tagacagaac tcagataaat ccgtattatc acgctttctt aatcacttga acaagtgatt
    11461 gtgctgcacc cgtagctcag ttggatagag tatctggcta cgaaccagag ggtcgggcgt
    11521 tcgaatcgct ccgggtgcgc cagt
//
LOCUS       DAWHRA010000063        11448 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWHRA010000063 DAWHRA010000000
VERSION     DAWHRA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11448
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.063"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..79)
                     /locus_tag="WIB30_001211"
     CDS             complement(<1..79)
                     /locus_tag="WIB30_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ3797282.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WIB30_001212"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WIB30_001212"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ3797283.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMVGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="WIB30_001213"
     CDS             complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="WIB30_001213"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ3797284.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSENKPAAKPTYTQVF
                     GKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLACE
                     GMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSFLR
                     CVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKGII
                     RREGGKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVTLE
                     ENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVERRV
                     RAWLSDRDAAN"
     gene            complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="WIB30_001214"
     CDS             complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="WIB30_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ3797285.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALSQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4798..5862
                     /locus_tag="WIB30_001215"
     CDS             4798..5862
                     /locus_tag="WIB30_001215"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ3797286.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            5882..6865
                     /locus_tag="WIB30_001216"
     CDS             5882..6865
                     /locus_tag="WIB30_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ3797287.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILCMTRS
                     KPVNRTTFCCLSLTTALILTACSSGGGGSGGGGVAADIGAGLADALTAPLDHKDKGLK
                     SLTLEDSIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKNDKISRFDFVQKIEVDGQ
                     TITLASGEFQIYKQDHSAVVALQIEKINNPDKTDSLINQRSFLVSGLGGEHTAFNQLP
                     GGKAEYHGKAFSSDDPNGRLHYTIDFTNKQGYGRIEHLKTPEQNVELASAELKADEKS
                     HAVILGDTRYGSEEKGTYHLALFGDRAQEIAGSATVKIGEKVHEIGIAGKQ"
     gene            7004..7681
                     /gene="tsaB"
                     /locus_tag="WIB30_001217"
     CDS             7004..7681
                     /gene="tsaB"
                     /locus_tag="WIB30_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ3797288.1"
                     /translation="MQADFNRPVLAIDTGTSHLSLALRADGETRLFHQEVGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYTKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHSELLYVRNKIALTAKEQ
                     AERKART"
     gene            7678..8118
                     /gene="rimI"
                     /locus_tag="WIB30_001218"
     CDS             7678..8118
                     /gene="rimI"
                     /locus_tag="WIB30_001218"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ3797289.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8112..8849
                     /locus_tag="WIB30_001219"
     CDS             8112..8849
                     /locus_tag="WIB30_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ3797290.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8911..9552
                     /gene="pyrE"
                     /locus_tag="WIB30_001220"
     CDS             8911..9552
                     /gene="pyrE"
                     /locus_tag="WIB30_001220"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ3797291.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9626..10060
                     /locus_tag="WIB30_001221"
     CDS             9626..10060
                     /locus_tag="WIB30_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797292.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10057..11367
                     /gene="argA"
                     /locus_tag="WIB30_001222"
     CDS             10057..11367
                     /gene="argA"
                     /locus_tag="WIB30_001222"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ3797293.1"
                     /translation="MSAPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASAAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
BASE COUNT         2601 a         3641 c         2963 g         2243 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccaaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatat agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat tctgtttaca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgcccaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acatcgcctt cgacttgagc ttcgtcttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgcc ggacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat ggtcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccagct ccaagcccgc accgccaaca
     1501 accgcgtcgt caactttacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa acccttacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgatgg
     1681 cgcggccagg cggcttaatt tgccgcatcc cgatccgaca accacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacaaagcgc
     1981 atatcggcga cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttcccacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg cgtctgcctg atagcaggtc gaaagcaaca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat gttcggcacg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catcccttcg caagccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caatgccgac gtcgaaatag cggtcgggga atcgttgttc aaactcaacc
     2521 aagccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcggc aggtttgttt
     2641 tcagacggca tttgcgccgc gctttctttg ggcaggttgg cgacggcgtg gtatttgacg
     2701 gggtcgtttt cggcaagttt gtagccgttg ccctttttgg tgatgacgtg cagcagttga
     2761 gggcctttgc gactgcgcaa gtctttcaat acgtccacca gattttcgac gttgtgtccg
     2821 tccacggggc cggtgtagcg gaagccgaag ttttcaaaca aagacagcga ctgtttggcg
     2881 tgttcggctt cttcggcaag ggttttgatt ttgtgttcga ctttttgggc aaactccatc
     2941 gcgccgggta ttttgtctaa taccttgccc gtttgcgctt tgacggtact caacaggccg
     3001 tgcatatcgc gcacgacgtt gctggcaagg tatttcggca gcgcgccgac gttgggggaa
     3061 atcgacattt cgttgtcgtt gaggacgacc agcaaatcca catccatatc gcctgcgcaa
     3121 ttcaaggctt caaacgcctg ccccgccgtc atcgcgccgt cgccgatgat ggcgacgctg
     3181 cggcggtcgc tgcccaagag tttgtctgcc gccgccatgc ccaacgccgc gccgatggag
     3241 gtggaggaat gccccacgcc gaacgcgtcg tactcggact cgcaacgttt cggaaaaccc
     3301 gccaaaccgc cgtattggcg catggtgtgc atctggtttt tgcgtccggt aaggattttg
     3361 tgcggatagc tttggtgtcc gacatcccac accagcttgt cttcgggcgt gttgtacaca
     3421 tagtgcaggg cgatggtcag ctcgaccgca cctaagttgc tggcgaaatg accgccggtt
     3481 tgaccgacgg attccagcag aaaggcgcgc aactcgccgg caaggcgcgg cagctgtttt
     3541 ttgtccagac ggcgcaaatc ttgcgggctg tcaatcaggt cgagtagggg gcttgggttc
     3601 atggtgtgtc ttttttatgt gtcgtccggg tgcaacggcc aattatatat caagagtgtg
     3661 cggctgacgg ctgattttgc cgtatgtcat tcgtcctgcc gcttggcgcg cgggtgggct
     3721 tcgtcataca ggcgggcgat gtggtcgaaa tcgagtttgg tgtaaatctg cgtggtcgaa
     3781 aggctgctgt gcccgagcag ctcctgcacc gccctgatgt cgcgcgaagc ctgcaacagg
     3841 tgtccggcgt agctgtggcg catcatatgc ggcgaaacgt gcctgccgtc gccgttttgc
     3901 gccgcccatt gcgccaaacg tttttggatt tggcgttggc tcaggcgcgt gccgttcctg
     3961 ccggtaaaca gggctttgcc gtccgatgcc gtctgacgca gcggcagata gtttttcagg
     4021 gcttccacgc ttttgccggt cagcggcacc tgccgctgct tgcgcccttt gccggtaacg
     4081 tgtacccacg cctcgtccaa atagacatca tctgcattca agccgtgtat ctcgctcacg
     4141 cgcaaaccgc tgccgtacat cagctcgaac agggcgtgat cgcgcaccgc cagcgggtcg
     4201 ccgccgtcca cgggcaaatc cagcatccgg ttcagccatt cctgcgacag ggctttgggt
     4261 acgcgctcgg gctgcttcgg cggtttgatg tcggcggtcg ggtcggtatg cagcagaccg
     4321 cgttccacca gccaaacaca atactgccgc caagacgaca gcttgcgcgc cagcgtccgc
     4381 gcattcaaac cgcgctgcga cagccgccgc aacgccgccg taaaatcgcg ccgcgacaag
     4441 tcctgcggca cgccgcctgc atcttcagac ggcatttgtg ccaacagcgc aaacagttct
     4501 tccaaatcgc gccggtatgc cgccaccgtg tgctccgact tgccctcgcg cacgatgttt
     4561 tccaaataag cgtcaaaatg cgccgcaaac ccgtccaaaa ccataaccgc cccacacaaa
     4621 tatcaaaaaa acagtgaaaa cattatcata aacgacacac caaaaccccg aaccgcccaa
     4681 aatccgaccc gtccgcccgc cgttatcgtt aacataaaat caatgaaccg tttatggttt
     4741 tttgccgcaa aaaacattat aatccgcctt atttacctat tgcccaagga gacacaaatg
     4801 gcactcgtat ccatgcgcca actgcttgat catgctgccg aaaacagcta cggcctgccc
     4861 gcgttcaacg tcaacaacct cgaacaaatg cgcgccatta tggaagccgc cgaccaagtc
     4921 aacgcgcccg tcatcgtaca ggcgagtgca ggtgcgcgca aatatgcggg cgcgccgttt
     4981 ttgcgccacc tgattctagc ggcagtcgaa gaatttccgc acatccccgt cgtgatgcac
     5041 caagaccacg gcgcatcgcc cgacgtgtgc caacgctcca tccaactggg cttttcctcc
     5101 gtgatgatgg acggctcgct gatggaagac ggcaaaaccc cttcttctta tgaatacaac
     5161 gtcaacgcca cccgtaccgt ggttaatttc tcccacgctt gcggcgtatc cgtcgaaggc
     5221 gaaatcggcg tgttgggcaa cctcgaaacc ggcgaagccg gcgaagaaga cggtgtaggc
     5281 gcagtgggca aactttcgca cgaccaaatg ctcaccagcg tcgaagatgc cgtgcgtttc
     5341 gttaaagata ccggcgttga cgcattggcg attgccgtcg gcaccagcca cggcgcgtac
     5401 aaattcaccc gtccgcccac aggcgacgtg ttgcgtatcg accgcatcaa agaaatccac
     5461 caagccctgc ccaatacaca catcgtgatg cacggctcca gctccgttcc gcaagaatgg
     5521 ctgaaagtca tcaacgaata cggcggcaat atcggcgaaa cctacggcgt gccggttgaa
     5581 gaaatcgtcg aaggcatcaa acacggcgtg cgtaaagtca acatcgatac cgacttgcgc
     5641 cttgcttcca ccggcgcggt acgccgctac cttgccgaaa acccgtccga cttcgatccg
     5701 cgcaaatatt tgagcaaaac cattgaagcg atgaagcaaa tctgcctcga ccgctacctc
     5761 gcgttcggtt gcgaaggtca ggcaggcaaa atcaaaccgg tttccttgga aaaaatggca
     5821 aaccgttatg ccaagggcga attgaaccaa atcgtcaaat aacaggttgc ttgtaaacaa
     5881 aatgccgtct gaaccgttgt tcggacggca tttgattttt gcttctttga cctgcctcat
     5941 tgatgcagta tgcaaaaaaa gataccataa ccaaaatgtt tatatattat ctattctgtg
     6001 tatgactagg agtaaacctg tgaaccgaac taccttctgc tgcctttctc tgaccactgc
     6061 cctgattctg accgcctgca gcagcggagg cggcggaagc ggaggcggcg gtgtcgccgc
     6121 cgacatcggc gcggggcttg ccgatgcact aaccgcaccg ctcgaccata aagacaaagg
     6181 tttgaaatcc ctgacattgg aagactccat tccccaaaat ggaacactga ccttgtcggc
     6241 acaaggtgcg gaaaaaactt tcaaagccgg cggtaaagac aacagcctca acacgggcaa
     6301 actgaagaac gacaaaatca gccgcttcga cttcgtgcaa aaaatcgaag tggacggaca
     6361 aaccatcacg ctggcaagcg gcgaatttca aatatacaaa caggaccact ccgccgtcgt
     6421 tgccctacag attgaaaaaa tcaacaaccc cgacaaaacc gacagcctga taaaccaacg
     6481 ctccttcctt gtcagcggtt tgggcggaga acataccgcc ttcaaccaac tgcccggcgg
     6541 caaagccgag tatcacggca aagcattcag ctccgacgac ccgaacggca ggctgcacta
     6601 caccatcgat tttaccaaca aacagggtta cggcagaatc gaacacctga aaacacccga
     6661 gcaaaatgtc gagcttgcct ccgccgaact caaagcagat gaaaaatcac acgccgtcat
     6721 tttgggcgac acgcgctacg gcagcgaaga aaaaggcact taccacctcg cccttttcgg
     6781 cgaccgcgcc caagaaatcg ccggctcggc aaccgtgaag ataggggaaa aggttcacga
     6841 aatcggcatc gccggcaaac agtagcattt gaaaaatacc gtccgaacac gataatttac
     6901 cgttcggacg gcatttttgc gccataccgc cctaggacgc gacggcacgg caggcaagcc
     6961 gatttcacta tataatgccg tctgaaccaa cgagaaccac accatgcaag ctgattttaa
     7021 ccgtcccgtc ctggctatcg ataccggtac ttcccatttg tcgctcgcgc tgcgcgccga
     7081 cggcgaaacc cgtctgttcc atcaggaagt cggcagccgc cagtccgaac tgattctgcc
     7141 ggaaatccgc actctattcc gaaatgcggg cattaccgcc gccgatttgg gggcgattgt
     7201 ttatacgaaa ggtcccggcg cgtttaccgg actgcgtatc ggcatcggtg tggcgcaagg
     7261 tttggcaacg ccgtttgata cccctttaat cggcatcccc acgctcgatg ccgccgcctc
     7321 gctgccgccg ccgcaaagct gcatactcgc cgctgcggac gcgcgtatgg gcgaagtgtt
     7381 ttatgcgtgg ttcgacacgc tgaaccgccg ccgtttgagc gattatcagg tcgggcgggc
     7441 ggcagacatc cggctgccgg agggatgtgc cttttcagac ggcataggca gcgcgttcgc
     7501 gttggaagaa acgccgccgt tttcaggcaa acccgatatg ccgaccgccg ccgacttcct
     7561 caatctggcg gcggaaggcg gttttcccgc cgtcgctgct gcacactccg aactgctcta
     7621 cgtccgcaac aaaatcgccc taaccgccaa agaacaggcg gaacggaaag cccgaacatg
     7681 aacatccgcc gcgcctcacc gtccgactgc gccgcactcg ccgcgctcga tgccgtctgc
     7741 aacccgtccg cgtggacgca acgccaattt gagtccgcat tggtttcgcc gtccgaacag
     7801 gttttcctcg cggaaaaaga cggcgggatt gccgccttta tcgtttggca gaacctgccc
     7861 gacgaatccg aactgcacct gattgccacc gcgcccgaat gccgccgccg tggcgttgcg
     7921 tccgccctgc tcgaatattg gttcgcacat ctgcccgaag acacgcaacg cctgctgctc
     7981 gaagtccgtg caggcaacac cgccgcacag gcactgtacg cggcgcacgg ctttaccgat
     8041 gcgggcaggc ggaaaaacta ttaccgtaca gccgacggta aaaccgaaga tgctgtctta
     8101 atggagaaaa tatgttaagc gcgcgctacc tccacctgca cgaagctttg ggtttaggcc
     8161 cgatgtggct gaaacaggcc gccgccgtcc tgccgcccaa aaacacaccc gcaaccccgg
     8221 cacaggcacg tcctcaaacc gtccgcgccg ccccgatccg cccttcccaa ccccataacg
     8281 gtcaggcgcg gctcgaaacg atgaaagcgt tggaaaccgc cgccgtacat acgcgcaaac
     8341 ccgcgcctga aaccgaaacg cccccgcccg gcctttcaga cggcatcgcc cccgttcccg
     8401 ccgcttcggg cataaccaaa cttgccgtcg tcagcctttg cccaccgatt gaggatgcgg
     8461 tttacgggca actgttctac ggcaaagcgg gtgttctgct tgacaacata ctcaaagccg
     8521 taggactgga tgccgcctat gtccacaaaa cctgttgggt gaaaaccgcc gccgtcggca
     8581 acccgatgcc gtctgaagcg gcaatcgaaa ccgcgacggt ttccgtcatg caggaactcg
     8641 acggctgccg cgcccccgcc gtcctattcc tcggacaggc ttttgtccag cctgaacggc
     8701 aaacgatgat tgaaactttg tgcggcagcc gtcccttctt catcatcgac catcccgccc
     8761 ggctgttgcg ccaacccgaa cttaaagccc gcgcctggca ggtgttgaaa cagttgaaac
     8821 gcgccttgcg gcaaggcggc ggcagttgaa gcgcaccgca cggggcggta gaatcgcaac
     8881 tgcgtcccaa tatctgacag aaagcacaaa atgaccgatt tccgccaaga tttcctcaaa
     8941 ttctccctcg cccaaaatgt tttgaaattc ggcgaattca ccaccaaagc cggacggcgg
     9001 tcgccctatt tcttcaatgc cggcctcttt aacgacggct tgtccacgct gcaactggca
     9061 aaattttacg cacaatccat cattgaaagc ggcatccgat tcgatatgct gttcggcccc
     9121 gcctacaaag gcattatttt ggcggcggca accgcgatga tgctggcgga aaaaggcgtg
     9181 aacgtcccgt ttgcctacaa ccgcaaagaa gccaaagacc acggcgaagg cggcgtgttg
     9241 gtcggcgcgc cgcttaaagg gcgcgtgctg attatcgacg acgtgatttc cgccggcaca
     9301 tccgtacgcg aatcgatcaa gctgattgaa gcggagggcg caacccccgc cggtgtcgcc
     9361 atcgcgctcg atcgcatgga aaaaggcacg ggtgagttga gcgcggttca ggaagtggaa
     9421 aaacaatacg gcctgcccgt cgcccccatc gccagcctga acgatttgtt tatcctgttg
     9481 caaaacaatc ccgaattcgg acagttcctc gaacccgtcc gagcctaccg tcggcagtac
     9541 ggcgtagaat aaaaacaaag catatgccgt ctgaaccgcc ttacgcctca gacggcatca
     9601 aacctgacac acacgaggaa ataccatgcc cgcctgtttc tgcccccact gcaaaacccg
     9661 tctctgggtc aaagaaaccc aactcaatgt cgcccaaggc ttcgtcgtct gccaaaaatg
     9721 cgaaggactg tttaaagcca aagaccatct ggcaagcacg aaagaaccca tattcaacga
     9781 tttgcccgag gctgtttcgg atgtcaaact cgttcaccgt atcggcacgc gcgccatcgg
     9841 caagaaacag atttcccgtg acgaaatcgc cggcatcctc aacggcggta caacccagcc
     9901 cgatattccg cccgcaaccg ccgccacccc tgctgccgca ccgcaggtta ccgtaccgcc
     9961 cgccgcgccc gcccgtcagg atgggttcaa ctggacgatt gcaaccctgt ttgcccttat
    10021 cgtcctcatt atgcagcttt cctacctcgt catcctatga gcgcgcccga cctctttgtc
    10081 gcccacttcc gcgaagccgt cccctacatc cgccaaatgc gcggcaaaac gctggtcgcc
    10141 ggcatagacg accgcctgct cgaaggtgat accttaaaca agctcgccgc cgacatcggg
    10201 cttttgtcgc aactgggcat caggctcgtc ctcatccacg gcgcgcgcca cttcctcgac
    10261 cgccacgccg ccgctcaagg ccgcacgccg cattattgcc ggggcttgcg cgttaccgac
    10321 gaaacctcgc tcgaacaggc gcagcagttt gccggcaccg tccgcagccg ttttgaagcc
    10381 gcattgtgcg gcagcgtttc cgggttcgcg cgcgcgcctt ccgtcccgct cgtatcgggc
    10441 aacttcctga ccgcccgtcc gataggtgtg attgacggaa ccgatatgga atacgcgggc
    10501 gttatccgca aaaccgacac cgccgccctc cgtttccaac tcgacgcggg caatatcgtc
    10561 tggctgccgc cgctcggaca ttcctacagc ggcaagacct tctatctcga tatgcttcaa
    10621 accgccgcct ccgccgccgt ctcgcttcag gccgaaaaac tcgtttacct gaccctttca
    10681 gacggcattt cccgccccga cggcacgctc gccgaaaccc tctcggcaca ggaagcgcaa
    10741 tcgctggcgg aacacgccgg cggcgaaacg cgacggctga tttcgtccgc cgttgccgct
    10801 ctcgaaggcg gcgtgcatcg cgtccaaatc ctcaacggag ccgccgacgg cagcctgctg
    10861 caagaactct tcacccgcaa cggcatcggc acgtccattg ccaaagaagc cttcgtctcc
    10921 atccggcagg cgcacagcgg cgacatcccg cacattgccg ccctcatccg cccgctggaa
    10981 gaacagggca tcctgctgca ccgcagccgc gaatacctcg aaaaccacat ttccgaattt
    11041 tccatcctcg aacacgacgg caacctgtac ggttgcgccg ccctgaaaac ctttgccgaa
    11101 gccgattgcg gcgaaatcgc ctgccttgcc gtctcgccgc aggcacagga cggcggctac
    11161 ggcgaacgcc tgcttgccca cattatcgat aaggcgcgcg gcataggcat aagcaggctg
    11221 ttcgccctct ccacaaatac cggcgaatgg tttgccgaac gcggctttca gacggcatcg
    11281 gaagacgagt tgcccgaaac gcggcgcaaa gactaccgca gcaacggacg gaactcgcat
    11341 attctggtac gtcgcctgca ccgctgaccg caacggaaag ccgccgcaga aatgccgtct
    11401 gaaccccgtt tcagacggca tttccccgat tatatagtgg attaacaa
//
LOCUS       DAWHRA010000064        11397 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWHRA010000064 DAWHRA010000000
VERSION     DAWHRA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.064"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(126..1391)
                     /gene="ftsY"
                     /locus_tag="WIB30_001223"
     CDS             complement(126..1391)
                     /gene="ftsY"
                     /locus_tag="WIB30_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ3797294.1"
                     /translation="MFSFFRRKKKQETPAPDEAQIQETAAKAESEVAQIVENIKEDAE
                     SLTESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETIS
                     EAVGQVKEDVATTEEHKLSWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            1537..3105
                     /gene="msrAB"
                     /locus_tag="WIB30_001224"
     CDS             1537..3105
                     /gene="msrAB"
                     /locus_tag="WIB30_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ3797295.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            complement(3207..3692)
                     /locus_tag="WIB30_001225"
     CDS             complement(3207..3692)
                     /locus_tag="WIB30_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ3797296.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(3718..4566)
                     /locus_tag="WIB30_001226"
     CDS             complement(3718..4566)
                     /locus_tag="WIB30_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ3797297.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            complement(4660..5661)
                     /locus_tag="WIB30_001227"
     CDS             complement(4660..5661)
                     /locus_tag="WIB30_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3797298.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            5720..6451
                     /locus_tag="WIB30_001228"
     CDS             5720..6451
                     /locus_tag="WIB30_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ3797299.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGLFTLINRAMTRYMTRRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDEVLTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPRKYERMLCVKPSLS"
     gene            6427..6699
                     /locus_tag="WIB30_001229"
     CDS             6427..6699
                     /locus_tag="WIB30_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ3797300.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPIYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            6754..8124
                     /gene="glmU"
                     /locus_tag="WIB30_001230"
     CDS             6754..8124
                     /gene="glmU"
                     /locus_tag="WIB30_001230"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ3797301.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVIGHGKDQVLNAVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     AKLAADVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWMR
                     PEKDKQ"
     gene            8201..8530
                     /locus_tag="WIB30_001231"
     CDS             8201..8530
                     /locus_tag="WIB30_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ3797302.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAVLQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNISCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(8600..9865)
                     /gene="efeB"
                     /locus_tag="WIB30_001232"
     CDS             complement(8600..9865)
                     /gene="efeB"
                     /locus_tag="WIB30_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ3797303.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYNYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(10129..11295)
                     /locus_tag="WIB30_001233"
     CDS             complement(10129..11295)
                     /locus_tag="WIB30_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ3797304.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGA
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRIHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            complement(11292..>11397)
                     /locus_tag="WIB30_001234"
     CDS             complement(11292..>11397)
                     /locus_tag="WIB30_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HEZ3797305.1"
                     /translation="GEALVWLLYLIPVITWFLCGSRPSETLTRKEELK"
BASE COUNT         2570 a         3370 c         2944 g         2513 t
ORIGIN      
        1 aaagtgggaa tccagaatct cggactttca gataaccttt gaatattgcc gttatcccaa
       61 ggtctggatt cccgcctgcg cgggaatgac ggcatcggtc tgctgttttc ggacggcatt
      121 tcggctcaat ccagcagtgc gtccacaaac gcgcgcgcgt caaacgggcg caggtcgtct
      181 atgccttcgc ccacgccgat atagcggacg ggaacggggc ggtcggaggc aagcgcggcg
      241 aggatgccgc cttttgccgt gccgtcgagt ttggtcacga ttaaaccagt cagccctaat
      301 gcgtcgtcaa aggctttgac ttggttgacg gcgttttgcc cgatgttggc atcaagcacg
      361 acgatgattt cgtgcggcgc gtcgggcatg gctttttgca gcacgcgttt cacttttttg
      421 atttcttcca tcaaatgaag ctgcgtgggc aggcggccgg cggtgtcggc cagcacaatg
      481 tcgatgccgc gcgctttggc ggcttggacg gcatcgaagc acacggcggc ggaatcgccc
      541 gtggtttgcg aaatcacggt aacgttgttg cgttcgcccc aagcttgaag ctgctcacgc
      601 gcggcggcgc ggaaagtatc gcctgccgcc agcaatacgg atttgccctg cgcttggaaa
      661 tatttggcga gtttgccgat agacgtggtt ttgcccgcgc cgttgatgcc ggcaagcatg
      721 atgacaaacg gctctttggt ttcgggcaaa accaaaggtt tctccagagg cttaatcagg
      781 tcgtacaagg cttctttcaa cgcgccgcgc aattcgttgc cgtctttcag ccctttgagg
      841 ctgacgcggt cgcgcacgtc tttcatcagg tattcggtgg cttccatgcc catgtcgctg
      901 gtaatcagca cggtttccag ctcttcgtac aaatcttcgt cgatttgtcc gccgccgaac
      961 acgcccgcca gcgatttcgc cattttgtcg cgcgatttgg tcaaaccttg tttcaaacgc
     1021 gccgcccaac tgagcttgtg ttcttcagtt gtcgcaacgt cttctttaac ttgcccgaca
     1081 gcctcgctga tggtttcggc aacagcttct ttcgccgctt cgacttgttc cgctgctttt
     1141 tccgccgctt cctctgcttc agacagcatc tcggcaacgg tttcctttac ctgttcaacc
     1201 gcaccgctga cggtttcaac ggcagattcg acctgccctt tgacgctttc cgttaaagat
     1261 tcagcatctt ctttaatatt ttcaactatt tgagcaactt cagactctgc ttttgctgcg
     1321 gtttcctgaa tttgagcctc atcgggagcc ggagtttcct gttttttctt gcgacggaag
     1381 aagctgaaca ttgaattttc cttttaattt tagaaacttg aaatagggag tattgtagcg
     1441 tattttacgc ggcaaggttg tctgaaaatc cgggctgtaa ggtttcggca tctcaaacgt
     1501 ctaatcatgc aaaccgtcca cacaggaaac atcaaaatga aacaccgtac tttcttttcc
     1561 ctttgcgcca agttcggctg cctgcttgcg ctgggtgctt gttcgcccaa aatcgtcgat
     1621 gccggagccg cgaccgtgcc gcacacttta tccactttga aaaccgcaga caaccgcccc
     1681 gccagtgttt acttgaaaaa agacaaaccg acgctgatta aattttgggc gagctggtgt
     1741 cctttgtgtc tgtccgaatt gggacagacc gaaaaatggg cgcaagatgc aaaattcagc
     1801 tccgccaacc tgattaccgt cgcgtctccg ggctttttgc acgagaaaaa agacggcgac
     1861 ttccaaaaat ggtatgccgg tttgaattat cccaagctgc ccgtcgtaac cgacaacggc
     1921 ggcacaatcg cccaaagcct gaatatcagc gtttacccct cgtgggcgtt aatcggtaaa
     1981 gacggcgacg tgcagcgcat cgtcaaaggc agcatcaacg aagcgcaggc gttggcgtta
     2041 atccgcgacc cgaatgccga tttgggcagc ttgaaacatt cgttctacaa acccgacact
     2101 cagaaaaagg attcaaaaat catgaacacg cgcaccatct acctcgccgg cggctgcttc
     2161 tggggcttgg aagcctattt ccaacgcatc gacggcgtgg ttgacgcggt atccggctac
     2221 gccaacggca acacgaaaaa tccgagctat gaagacgtgt cctaccgcca tacgggccac
     2281 gccgaaaccg tcaaagtgac ctacgatgcc gacaaactca gtctagacga catcctgcaa
     2341 tatttcttcc gcgtcgttga tccgaccagc ctcaacaaac agggcaacga caccggtacg
     2401 caataccgca gcggcgtgta ctacaccgac cccgccgaaa aagccgtcat cgccgccgcc
     2461 ctcaaacgcg agcagcaaaa ataccaactg cccctcgttg ttgaaaacga gccgctgaaa
     2521 aacttctacg atgccgagga ataccatcag gactacctga ttaaaaaccc caacggctac
     2581 tgccacatcg atatccgcaa agccgacgaa ccgctgccgg gcaaaaccaa gaccgccccg
     2641 caaggcaaag gcttcgacgc ggcaacgtat aaaaaaccga gtgacgccga actcaaacgc
     2701 accctgaccg aagagcaata ccaagttacc caaaacagcg cgaccgaata tgccttcagc
     2761 cacgaatacg accatttgtt caaacccggc atttatgtgg acgttgtcag cggcgaacct
     2821 ttgttcagct ccgccgacaa atatgattcc ggctgcggct ggccgagctt cacgcgcccg
     2881 attgatgcaa aatccgttac cgaacacgat gatttcagct acaacatgcg ccgcaccgaa
     2941 gtgcgcagcc acgccgccga ttcgcatttg ggacacgtct tccccgacgg cccgcgcgac
     3001 aaaggcggac tgcgctactg catcaacggc gcgagcttga aattcatccc gctggaacaa
     3061 atggacgcgg caggctacgg cgcgttgaag agtaaagtga aataagccgc accgccgcct
     3121 aacccgacaa aataccgtct gaaacctgca acgtttcaga cggtattttt tatccggcgg
     3181 ggattctgtc cagacggaga ttttgtttag acagcatcgc cgccgttttc aatcagcccc
     3241 gccaaccgtt ccaacgcgaa ggcgaccgcc tgcgcgcgga cggattcgcg gttgccgtca
     3301 aaacggcgca ttgcttcgca acttccgccc ggaaaggcaa acccgaacca aaccgtgccg
     3361 acgggtttgc tttcgctgcc gccgcccggt ccggcgatgc cggaaatacc gacggcgtaa
     3421 tccgcctgcg ccacggcttt cgcgccgcgc gccatctcat agacggtttg gcggctgacc
     3481 gcgccgtgtt cgagcagggt ttcgggcaac acgcccaagc ggtcttcttt ggctttgttg
     3541 ctgtatgtta caaaactttg atcgaaccat tgcgaactgc ctgcaacgct tgtgaatgcg
     3601 gcggcaagca ttccgcccgt acaggattcg gcacagctta cggtttgacg ttttttcgtc
     3661 aggtttcggg cgatggtgtg cagcgcgtcc atttcccact cccctttcag acggcgttta
     3721 agaattgatg atgtgtatgt cgcgttgcgg gaacgggatg ttgatattga ctttgcggag
     3781 gttttcgacc acttgttcgt tcaagtcgca ttgcagcgtc cagcggtctg cttcgtttgc
     3841 ccaagcccat aatgtgattt cgatggcatt gtcgcccaag gcggtgatgt aggcggcggc
     3901 ctgccgctct tcgttttgaa cgctcaaggg gtgttcgacg gcggctttca acaccgcctc
     3961 tttcgccact ttcaaatcgc agttgtaatc gacgccgact atcacttggg cgcggcacag
     4021 cggcagtgtg gaacggttga cgatgctgtt gcccatcacc acgctgttgg gcagcacgac
     4081 ttcttcgttg tcggtcgtcc gcaaagaagt ctgcaccatt ttaatctctc ggacatatcc
     4141 ttcaaaaccg ccgacgcgga taaaatcgcc gactttgaac gggcggaaca ggataatcag
     4201 cgcgccggcg gcaaaattgg acagctggtc tttcaaggac aacgccaccg ccaaacccgc
     4261 gccgccgatt aaggcggtta cggatgttgt ggaaacgccc aatctgccca atgcggcaat
     4321 aatcaccaaa atcaataagc cgatattggc aacattacac aaaaaactaa tcagcgtggc
     4381 atcgacctgc gcgcgcgtca tcgccgccct catcacagcg acaatgcgtt tcgccgccca
     4441 ttttccgacc aaaaaaataa gcagcgcggc ggcaaggttc agcccgaacg cccacgcctt
     4501 ttcagccaga tgctcccaac cggaaacact gatcaggtgt aaaaaatcaa attgtttgaa
     4561 gtccattgtt ttcctcttga tcgaacatcc gccccgtgcg gcgtaatcgg cacaggtgta
     4621 aaaatgccgt atgaagccct gcggggcggt atgtttgttt tatttcaaaa ccgttctaat
     4681 ccaacgggaa acccatccgc gctggttcgc atcaatatcg cgctgcgcgg gggcggtggt
     4741 gtgcgtcggg gcttgggcga agcggaacac gtcgggcagg cgcgtgtttt tgacggcggg
     4801 gtaaacccac atttcggacg gaaccgcctg ctgcacttcc cgactttgca gccattgcac
     4861 cagttttgcc gccaattccg gctgtttcgc gcccttcaag accgccgcgc cttcgacctg
     4921 gcggaatacg ccgcctttta aaaacaggtt gcccgtcggc ggctcgctgt atttgccttt
     4981 ggaaaaatac acttccgccg ccgggctggc ggcataaccg accaccagcg gatacgcgcc
     5041 gccgttgtgc gaaaagtcgg tgtaatacgc ctcgctccag cctttggcga ccttcacgcc
     5101 gttctgccgc atctgtgccc accatttgaa cgcgccttct tcgcccagac cgctgatgtt
     5161 cgccatcagg aagcccagcc ccggggacga cgtggcgggg gacggcacga ccaataggtt
     5221 tttatattcg gggcgggtca aatcctgcag ggtttgcggc aggggcagct ttttgccttc
     5281 aaaccatttt ttgtcgtaat tgatggacac atagccgtaa tcgaccgcca aagccgaagg
     5341 cagcccgacc gcgacggggg cggattcggg ttgcgccgcc gccaaaatgc ccatttcccg
     5401 cgccttgccg atattggcgt tgtccaaacc gtacaccgcg tcggcaatcg ggttggcgcg
     5461 gctcaaaatc agtttgttga gcatttcgtt cgcgccgccc gcctgaataa tcgacacctt
     5521 cgcatcgttt gcccgctcga agcgcgcaat cagccctttg ggcaggctga acgacttatg
     5581 caccgccagc ctgacttccg tctgcgcctg caggtatgcc gaaaccgcca gcagcggcag
     5641 cagccaaatt ttccgtttca ttccgagtcc tcctctattc gctgtaaaat aacattctaa
     5701 caaattttca cggttcaaaa tgcaagaaaa cccgaccgtg tggctgttcg acctcgacaa
     5761 cacgctgcac aatgccgatg ccggcctctt cacactcatc aaccgcgcta tgacacgcta
     5821 tatgacacgc cgcctcaaac tctccgaatc cgccgcgtcc gacctgcgtc aagactattg
     5881 gcaccgctac ggcgcaacgc tcgccggact gcaaatccac catcccgaaa tcgacatcgc
     5941 cgaatttttg cgcgaaagcc atccgattga cgaggttttg acccgtctgc acggtatgcc
     6001 ggaaacacaa aacaccctga gccgcctaaa agggcgcaag gcggtttttt ccaacggccc
     6061 gtcgttttac gtccgtgccg ttgtcggcgc actcggtttg gaaaaccgtt tcgacgcgct
     6121 tttcggcacg gatgatttcg ggctgctgta caaacccaat ccgcaagcgt atctcaatgt
     6181 ctgccgcctg ttggacgtac cgcccgaatg ctgcattatg gtggacgaca gcgcggacaa
     6241 cctgcatcag gcaaaagcat tgggcatgaa aaccgtccgg ttcggtgcaa aagtgcacga
     6301 cctgcccttt atcgatgctt ccgtaagcga tatggcgcaa ctggctcggt atgcagaaac
     6361 tttgtcagaa caccgccaaa atcattacaa tacccgcaca accccccgaa aatacgaaag
     6421 aatgttatgc gtaaaacctt ccttatcctg atgactgccg ccgccctttt gtcgggctgc
     6481 gcgtgggaaa cttatcaaga cggcaacggc aagaccgccg tccgtcaaaa atatcccgcc
     6541 ggcacgccca tttattacca agacggcagc tactcgaaaa atatgaacta caaccaatac
     6601 cgtcccgaac gccatgccgt gttacccaac caaaccggca acaacgccga cgaagagcat
     6661 cgccaacact ggcaaaagcc caaatttcaa aaccgataaa cccaccctat gccgtctgaa
     6721 gccctttcag acggcattgc acaggaaacc gtcatgccgc aaaacacttt aaacatcgtt
     6781 atcctcgccg ccggcaaagg cacgcgcatg tattccaaaa tgcccaaagt gctgcaccgc
     6841 atcggcggca agcccatgct cgggcgcgtt atcgacaccg ccgccgcgct gaatccgcaa
     6901 aacatctgcg tcgtcatcgg gcacggcaaa gaccaagttt taaacgctgt caaacgcgat
     6961 gtcgtttggg ttgaacaaac cgaacagctc ggtaccggcc acgccgtcaa aaccgccctg
     7021 ccccaccttg ccgccgaagg ccgcacgctg gtgttgtacg gcgacgttcc tttaattgac
     7081 gttgaaaccc tcgaaaccct gctcgaagcc gcaggcaacg aagtcgggct gttgaccgac
     7141 gtgcccgccg acccgtcagg tttggggcgc atcatccgcg acggcagcgg cagcgtaacc
     7201 gccattgtcg aagaaaaaga cgccgacgcc gcccaaaaag ccgtgaaaga aatcaatacc
     7261 ggcattttgg ttctgcccaa cgccaaactg gaaaactggc tgaacagcct ctccagcaac
     7321 aatgctcaag gcgaatatta tttaaccgac ctcattgcca aagccgttgc cgacggtatt
     7381 aaagttcatc ccgtccaagt ccgcgcctcc cacctcgccg ccggcgtgaa caacaaacgc
     7441 cagctcgccg aactcgaacg catcttccaa accgaacagg cgcaagaatt gctcaaagca
     7501 ggcgtaaccc tgcgcgatcc ggcacgtttc gatttacgag gccgtctgaa acacggacag
     7561 gatgtcgtaa ttgatgtgaa ctgtatcttt gaaggcgaag tcgaactcgg cgacaacgtc
     7621 gaaatcggcg caaactgcgt catcaaaaac gccaaaatcg gcgcaaacag caaaatcgcc
     7681 cccttctccc acctcgaaag ctgcgaagtc ggcgaaaaca accgaatcgg cccctatgcc
     7741 cgcctgcgtc cgcaagccaa actagccgcc gacgtacacg tcggcaactt cgtcgaaatc
     7801 aaaaacgccg ccatcggcaa aggcaccaaa gccaaccacc tcacctacat tggtgatgcc
     7861 gaagtcggca gcaaaaccaa cttcggcgca ggtacgatta ttgccaacta cgacggtgtg
     7921 cacaaacaca aaaccgttat cggcgacgaa gtccgcatcg gttcaaactg cgtcctagtc
     7981 gcccccgtta ccctcggcaa caaagtaacc acaggcgcag gcagcacgat tacccgcaat
     8041 atcgaagaca acaaactcgc cctcgcccgc gcccgccaaa ccgtcatcga aggctggatg
     8101 cggcctgaaa aagacaagca ataaagctat gccgtctgaa gccggtttca ggtttcagac
     8161 ggcatcaaac tcctccgata tcagataagg actgcaaacc atgtcattac ccccatgccc
     8221 acaatgcgcc tccgaataca cttatgaaga cggcggacaa tacatctgcc ccgaatgcgc
     8281 ccacgaatgg aatgaaaccg aatccgccgc cgaccttgcg gctcaagtgc gcgatgccaa
     8341 cggcgcagtg ctgcaaaacg gcgacaccgt catcctcatc aaagacctca aggtaaaagg
     8401 cagctcgatg acgatcaaac aaggtacaaa agtcaaaggc atacgcctac aggaaggcga
     8461 tcacaacatc tcctgcaaaa tcgacggcag cgcgatgaat ttaaaatctg aattcgtcaa
     8521 aaaagcctga ccgcccaaac actaaaatgc cgtctgaacc ccttatcgga ttcagacggc
     8581 atttgtccgt caggcaggtt tatacgccca gcagcccttg tcccaagaag ccgccttttt
     8641 ccacgccggg cagcacgaag aaatagccgc cgccgaaggg gctgatgtat tcttccagcg
     8701 gttcgccgtt gaggaggttt tgcacgaaga tgaagccgtc tgcaaggttt gcctgataac
     8761 agacgaacac cagcccgaca tcaagctgtc cgcttgaggc gagtccgcgc gaatagttgt
     8821 aggcgcggcg gaagaggcgg tgttttttaa ggaactcggg atcgcgcgga ttcgccaggc
     8881 gtatatggct gtctttgggc gtggtattcc cctcggggtc tttggcaaaa tccggttggt
     8941 cggcttcttt tttgccgtcc atcggcgcgc cgctgtattt gcgccgcccg aaaatgtcgg
     9001 tttgctcttg aagcggcgtc ctgtcccaaa actcaacaaa gtggcggata aggcggactg
     9061 cctgatagct gccgtttttc gcccactccg gttcgtcgag gctgttggcg gccacccccg
     9121 tccacaaaac ctcgtcggca gttttggggt cggaaacttt ggggttaccc gtgccgtcgc
     9181 ggaagcccaa caggttgcgc gccgccatcg cgccgggttc ggatttaggc tgccacccgt
     9241 cgatactcca gcggataacg gcggtttgga cggtgtgttt gatgatgtcg cgcagggcgg
     9301 cttggcaggt ttcgggggtg aaggcacaga tttgcaggct caaatcgccg tcgcaccagc
     9361 ttttttgcag cttatcgttg gagaagtcgc gcatttcctg caaatgaatc ggttttttgt
     9421 ctttgagtcc gaaccggccg tcaaacaggc tgctgcccac ccccacggta acggtcaacc
     9481 cgtcggggtt gaaggctttg cccaaaatgc cgctgccggc tggcggaagt ttgtcgtcgc
     9541 cgtcttggta ttcgccgcct tgggtgagaa actcgatgcg ggcggtcagc gtgcggaaca
     9601 ggttttccag ctgcttggca ctttgcgcgg ttacgtcgaa ggcgcacata atcgaaaacg
     9661 cctgctgcgg cgtaacgatg cctgcctgat gttcgccgta gcagggatag gcttggggcg
     9721 agtgttggct ttcggcggtg cgttcggcgg tttcgccctg ttttttgccg ccgagataac
     9781 ctccgattgc gccgactgcg ccggctgcga tcgcggtttt aaaaagagtg cgcctggtcg
     9841 gttgtgcggg ttggtttttg ctcatggtgt ttccttcaat atccgtgcag caggatgggt
     9901 atccctgcac gccgtttttc cattaaccga tagcggcagg ttgaaatccc gccctgcaat
     9961 atatggcgga tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
    10021 taaatagtac ggcaaggcaa ggcgaggcaa tgccgtactg gtttttgtta atccactata
    10081 aaacttcaga cggtatctgc cgccaaatac cgtctgaacg cttgcggctt atttcaagcc
    10141 gagtatgccg cgaagttggg caaggtcttc ggcaagcgcg ttaatagagg cctgtaacgc
    10201 tttgcggtcg gcttcgccca gcttgtcgta ggtttcaaaa ccgtctttag tccggtattt
    10261 cgccagaatt tcgttgacct gtttgaagtt ggtatcggtt ttttccaaca aggctttgtt
    10321 tttggcctcg atcagcggac ggaacaaatc gacgattttt ttcgaaccgt ccacattggc
    10381 ttggaaatcg ctcaaatcgg tgtggctgta ccggtcttct tcgccgctga ttttactgcc
    10441 cgccacttct tcaatcagtt cggacgcgcc gccgaccacc ttgcccggag gaaacgccaa
    10501 tgcgtcgatt tctttttgca gggcttcgac atcggtcatc agtttcgctg caatttcctt
    10561 cacgccggac acgtcttttt ccacccaaag ggcgtattcg atacgatgga agccggtaaa
    10621 tccggcatct ttcgcgccgt ctttgaagtc gtcttcacgc gcatcgatga cggggtcgag
    10681 ttcgctgaaa agctcggcaa tcggttcgat gcgttcgtaa tggatgcggg tgtcggcaaa
    10741 cagggatttc gccttttcaa tgtcgcctgc tttgacggct tcggtaaagg ttttggtttt
    10801 cgccaccagc tctttaacct cgccttgaac gtaggcttta tagtcggcga gcggttggga
    10861 cagtttttcc aaatccgctt cgttggcggt gtctttaaag ccgctgtcgg ttaccaccag
    10921 cttgccgcgc ggattggtca aaaggccgca ggtcatttcg tattcgcccg gcaacagggt
    10981 gacggtcatt ttatcggaaa gtccgggggc gatgttttcg cgctcgtcca ccaccatcac
    11041 gcccttcagg atttcccatt cgagcttgcg gccgctgttg tttttaatat tgaacacaac
    11101 ctgtccgctc ggcacggtca gttccatcgg ttcgcaggca ttgtcgttga cggcgatact
    11161 gaccgcaccg ccctcgttgg cggtttgcgc ctcaccggac gctgccggcg cagctttctc
    11221 cgcctccggc ggctggcacg cggtcaaacc taaggcaagc atcacggaca atgcggtcaa
    11281 attgaatttt ctcatttcag ctcctcttta cgggttaaag tttcagacgg cctgctgccg
    11341 cacaaaaacc aagttatgac gggaataagg tacagcagcc aaaccaaggc ctcgccc
//
LOCUS       DAWHRA010000065        11295 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWHRA010000065 DAWHRA010000000
VERSION     DAWHRA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.065"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..498)
                     /gene="dnaX"
                     /locus_tag="WIB30_001235"
     CDS             complement(<1..498)
                     /gene="dnaX"
                     /locus_tag="WIB30_001235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ3797306.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTV"
     gene            complement(593..2569)
                     /gene="mutL"
                     /locus_tag="WIB30_001236"
     CDS             complement(593..2569)
                     /gene="mutL"
                     /locus_tag="WIB30_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ3797307.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPEQSLHERIAAIVGDDFQT
                     ASLEIDSGSGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDRASNYPTGNKPDTRNAFGASGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSPSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGKLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLNELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            2712..2897
                     /locus_tag="WIB30_001237"
     CDS             2712..2897
                     /locus_tag="WIB30_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ3797308.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            2908..3576
                     /locus_tag="WIB30_001238"
     CDS             2908..3576
                     /locus_tag="WIB30_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ3797309.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPRYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            3576..4292
                     /locus_tag="WIB30_001239"
     CDS             3576..4292
                     /locus_tag="WIB30_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ3797310.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            4340..4825
                     /gene="purE"
                     /locus_tag="WIB30_001240"
     CDS             4340..4825
                     /gene="purE"
                     /locus_tag="WIB30_001240"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ3797311.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(5008..6462)
                     /locus_tag="WIB30_001241"
     CDS             complement(5008..6462)
                     /locus_tag="WIB30_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ3797312.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(6459..7160)
                     /locus_tag="WIB30_001242"
     CDS             complement(6459..7160)
                     /locus_tag="WIB30_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ3797313.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(7157..7936)
                     /locus_tag="WIB30_001243"
     CDS             complement(7157..7936)
                     /locus_tag="WIB30_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ3797314.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            8221..9627
                     /locus_tag="WIB30_001244"
     CDS             8221..9627
                     /locus_tag="WIB30_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3797315.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLDMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQIAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWVFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVAGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGTNLTRNTPS"
     gene            9739..11265
                     /locus_tag="WIB30_001245"
     CDS             9739..11265
                     /locus_tag="WIB30_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ3797316.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPAPRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         2387 a         3111 c         3097 g         2700 t
ORIGIN      
        1 gacggtaacg ggaactttgt gcggatcggt ggtggcgagg atgaatttga cgtgttcggg
       61 cggctcttcc agcgttttga gcatagcgtt gaacgcgctt ttggaaagca tatgcacttc
      121 gtcgatgata tagactttgt attttccggc ggtcggtgca tattgggcgt tttccaagac
      181 ttcgcggatg ttgtcgatgc ctgtgttgga ggcggcgtcg atttccagca ggtcgacgta
      241 gcgtccggca tcgatctgcg tacagctttc acatacgccg caaggttcgc cgtgttgcgc
      301 gttttcgcag ttgaggcttt tggcaaggat gcgggcgatg gtggttttac ctacgccgcg
      361 cgtgccggtc agcaggtagg cgtggtgcag cctgccttcg tccagggcgt tttgcagggc
      421 tttgacgacg tgttcctgac cgactaagtc ggcaaaggtt ttgggccgcc attttcgggc
      481 gagaacttga taggccatgt ttttctcttg gtttcggtcg tgatgtttct gtcggtgcgt
      541 cggaatgccg tctgaacggc ggtctcgggc ggcgtattct agcactttcg gcttactgtc
      601 cgcgcaagaa cagtgcgtcc aattctttca aagtcagttt gacccaagtc ggcctgccgt
      661 ggttgcactg gttgctgcgc ggcgtatttt ccatatcgcg cagaagggcg ttcatttcgg
      721 gcagggtgag ccggcggccg gcgcggatcg agccgtggca ggacatggtg gcgaggatgc
      781 ggttttcgtg ttcctcgatg gtttggctgc tgccgacttg ggcgagttcg tttaatacgt
      841 ctttggcgag cgagacgaca tcggctttgc cgagcatggc gggaactgca cggacggcga
      901 gggtgttgcc gcccatatcg gataattcca gcccgaagcc tgccagcgtt tcggcataat
      961 cggcaagggc ggcgcattct tcgtgggacg cggcaaaggt tacgggaata agcaggcgtt
     1021 ggctttgcag cttgccgttt tcctgccgtt ggcgtttcat tttctcgtag ttgacgcgtt
     1081 cggcagcggc gtgcatatcg atgagcagca ggctgtcttc ggcttgggca aggatgtaga
     1141 tgcctagcaa ttgggcaatg gcgaaaccga gcggcggcag ttcggatggg gacgggatgc
     1201 cgtctgaaag cggcgtatct gtttggggag caggcgtttc agacggcata ttgccgaaac
     1261 gtgcctgctc gaatcggctt aactcaaggt cgatgtcgtc ggtttttttg tagagttcgg
     1321 cataggtgtt cattgccgcg cggctttcgc gcagggacag gctgcgttgt tgcggcgcat
     1381 atgcggactg atagggcata ggcgcggttt tgcctgatgc accaaaggca ttgcgcgtat
     1441 cgggtttgtt gcctgtcggg tagttggatg cgcgatcaaa cagattttcg ctgtcgtttt
     1501 cagacggcat tggggcaggc gtaacgccgg taatgtcatg caacacttcg cctgcgttgc
     1561 tgacgctttc ggtcaagtcg gcgcgtgtgt cggcaagggc tttgttgagc gtgtggaaca
     1621 cgagttggtg cacctgctgg ctgtcgcgga agcggatttc ggttttggtc gggtggacgt
     1681 tgacatccac ggcttcgggc ggcaggtcga ggaaaaggac gaaagcgggg gtgagggcgt
     1741 tgtgcaaaac gtcgcggtat gcctgcttga cggcgtggag catcactttg tcgcggacaa
     1801 aacgatggtt gacgaagcag tattgtttgt cggttttgcc tttggcgaaa gtcggcttgg
     1861 caatcgcgcc atagagccgc aatgcgccgc tgccgctgtc gatttccaaa gatgccgtct
     1921 gaaaatcatc gccgacaatg gcggcaatcc gttcgtgcag gctttgttca gggagtttga
     1981 acacttgttt gccgtcgcgt ttgagcgaga aggcaatgtg cggatgcgcc agcgcgaggc
     2041 gttcgagcat agtggcgcag tgggcgtatt cggtggcttc agacttgagg aacttgcgcc
     2101 gcgcgggggt gttgaagaag agttcggcgg cttcgatggt ggtgccgacg gggtgggcgg
     2161 cggcggtggg gctgctgagt ttgccgtctt cggctttgac ttgggttgcg tgcgaactgt
     2221 cttcttgacg gctggtcagg gtcaggcggc tgacggaggc gatgcttgcc aagccttcgc
     2281 cgcgaaagcc catgctggca acgtgttcta aatcgtttaa ggttttgatt ttgctggtag
     2341 cgtgtcggtg gagcgcaagc tcgatgtcgt ccggatgaat accgctgccg ttgtcgctga
     2401 cgcgaatcag gcggatgccg ccgcccgcca gctcgacatc aatcgccgtt gcgcctgcat
     2461 cgatactgtt ttcaacgatt tctttcaagg cgttggcagg gcgttcgacc acttcgccgg
     2521 cggcgatttg gttgacaaga tggtcgggca gggcggcaat gcgtggcata aggtaggctt
     2581 ccgttgcaaa aacgctttat tctataataa aaccccttac ttttctgcct gtattcttga
     2641 taaaagccgt ttatcccgtt tggaaaatac cagtataatc acgccgtatc ccgtaaaatt
     2701 ggagcgcaaa gatgtatcac taccaatccg aagccacaca attcctcaac cgcctgattg
     2761 aagaaaaacc tgagttggaa cagcagcgtt tggaaaaccg ggggcttttg tgggatgtcg
     2821 aactcaatcc cgaagaacag gaaaacttcg aggcggcaaa agtagcgaaa aaaccttata
     2881 cctattacca agactgaacc cgccgaaatg acgacccatc tgtccaatgt cgcaccggac
     2941 ctgcaaaact atctgaacag catcggcgaa cccgaacatc ccgttttgac gcggctgcgt
     3001 gagaagaccg agcatcaccg tatgggcaaa atggcgattg cgcgcgaaca ggcggcggtt
     3061 ttggtttggc tggcaaagct gattcgcgcg gaaaaatatc tggaagtcgg cgtgtttacc
     3121 ggatacagca gcaccgcgct tgcattggcg ttgccagaac acgggcggat taccgcctgc
     3181 gacatcaatg taacctttac cgatacggcg cgtcaggttt ggaacgaggc aggtgtggca
     3241 cataaaatca gcctgcacct gcaacccgca ttgctgacat tggatgattt gattgcacag
     3301 ggtgaagccg gaagctacga tttggcactg atagacgcag acaaaccgcc cacgccgcga
     3361 tatttcgagc gttgcctcaa gcttgtccgt caaggcggca tcatcgccat cgacaatatt
     3421 ttgctgaacg gaagggtgat gcgcgaagcg gcttccgatg cgccgcccag cgtcggcatc
     3481 ctcaaagatt tcaatcaaaa cctgccgaac gatacgcgga ttgtccccat caccctgccc
     3541 gtcggcgacg gcttgaccct gcttctgaaa aaataatgaa gaccaaatta ccgcttttta
     3601 tcatttggct gtccgtatct gccgcctgtt cttcccctgt ttcccgcaat attcaggata
     3661 tgcggcccga accgcaggca gaggcaggta gttcggacgc tattccctat cccgttccca
     3721 ctctgcaaga ccgtttggat tatctggaag gcacactcgt ccgcctgtcg aacgaagtgg
     3781 aaaccttaaa cggcaaagtc aaagcactgg agcatgcgaa aacacaccct tccagcaggg
     3841 catacgtcca aaaactcgac gaccgcaagt tgaaagagca ttacctcaat accgaaggcg
     3901 gcagcgcatc cgcacatacc gtcgaaaccg cacaaaacct ctacaatcag gcactcaaac
     3961 actataaaag cggcaggttt tctgccgctg cctccctgtt gaaaggcgcg gacggaggcg
     4021 acggcggcag catcgcgcaa cgcagtatgt atctgttgct gcaaagcagg gcgcgtatgg
     4081 gcaactgcga atccgtcatc gaaatcggag ggcgttacgc caaccgtttc aaagacagcc
     4141 caaccgcgcc cgaagccatg ttcaaaatcg gcgaatgcca atacaggttg cagcagaaag
     4201 acattgcaag ggcgacttgg cgcagcctga tacaggctta cccgagcagc ccggcggcaa
     4261 aacgcgccgc cgcagccgtg cgcaaacgat agtttttcca tttttccgct ttttacccca
     4321 tccgcaccaa ggaggattta tgatacaaat cggcatcatc atgggcagca acagcgattg
     4381 gcccgttatg cggcaggcag cgcagtttct tgaagagttc ggcgtagaat atgaggcgcg
     4441 cgttgtttcc gcacaccgca ccccggattt gatgttccaa tacgccgaaa ccgcacgggc
     4501 gcgcggcatc aaagccatca tcgcgggtgc gggcggcgcg gcgcatttac ccggtatggt
     4561 tgccgccaag accaccgtcc ccgttttggg cgtacccgtc cccagcaaat acctgcgcgg
     4621 cgaagattcg cttttatcga ttgtacaaat gcccaaaggc gtacccgtcg ccacattcgc
     4681 catcggcgag gcaggcgcgg caaatgccgc attgttcgcc gtttccatgc tcgccaacga
     4741 aacccccgaa ctggcgcaaa aactggcaga cttccgagcc aaacaggaac aggctgtttt
     4801 gagtatggaa ttggaacaga tttaaaaccc gccgtccgcg aatatatagt ggcttaaatt
     4861 taaaccagta cggcgttccc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg
     4921 ccttgtcctg atttttgtta atccactata caaatgccgt ctgaagccct gtttcgggtt
     4981 tcagacggca tttttgcgaa caacttttta tgcctgttct ttttggcgca ttttttctgc
     5041 cattagttcg tgcagggttt tcttcgccgg tttcatcggc acgcggtttt gcgtccaacc
     5101 caactgtttg cgcggggtca ggttgcggaa cttggtggct gcccaaccga aggcgcggta
     5161 ggttttgctg ccgctgaaaa taccgttgaa tgtgcgccac gccatttgtt cgccgaaggt
     5221 atgcgatgcg ccttgccctc ggatggggtg cggcacggtt tcggtcggcg aacgttgcgc
     5281 ttcaacgcgc aaacgctgca tttgttcggt aatcgggatg cgtaccggac agacttccac
     5341 acacgcaccg cacatggtac aggcggtcgg caggtcgcgg gtggcatcca gacccaacag
     5401 gtgaggggaa ataatctcgc caatcgggcc gggataggtt gtgccgtatg ccgcgccgcc
     5461 gatgcgggta taaaccgggc aatggttcat acacgcgccg caacggatac attgcagggt
     5521 gcggcgcatt tggtcttcgg cataagcctg gctgcggccg ttgtcgagca gaaccaagtg
     5581 catttcttgc ggaccgtcta attcttcact gcggcgcggg ccggtaatca tattgaaata
     5641 ggttgtgatg ttctgaccga tggcggagcg cggcagcaga ctgtacaagg gtgggatgtc
     5701 ggacaatttc gccaccactt tttcaatgcc ggtaatagcg atatgcacgg gcggaacggt
     5761 ggtactcaag cgaccgttgc cctcgttttc caccagacac agcgtacctg tttcagcaac
     5821 ggcaaagttt acgccactca agccgacatc ggcagtgctg taaatatcgc gcagggcttt
     5881 gcgggcgaag ccggtcagtt ggtccacatc gtctgtaagc ggtgtgccga ggttttggtg
     5941 gaacagttcg ctcacctgtt ctttggtttt atggattgca ggcatcacga tatgggtcgg
     6001 tttttcgcct gccatttgga cgatgaactc gcccaagtcg ctttctaccg ctttaatgcc
     6061 ttttgcttca agataatggt tcagctcgat ttcctcgctg accatcgatt tgcctttgac
     6121 catcagcttg ccgtttttgg ctgtgatgat gtcgtggata atttggcagg cttcggtcgg
     6181 ggtttctgcc cagtgcactt tcacgcccaa cttagtcagg ttttcttcca gctgctccag
     6241 cagggctggc aatttagaca atgagcgctg acggacgtgt tcgcacaaat cgcgcaggct
     6301 ttgcagctct tcttcgtcgg tcaaaacggc tttgcgtttg gtcatcagca tatccatcgc
     6361 ggtacgcagg cttttgcgca aaggcttgtc ttgaagggaa attgcggcgt tttgcttgaa
     6421 agtttccggc ttcatgtgaa atttgatggt ttgcgtagtc atgcgttttc ctccaaatcg
     6481 gcaggggaaa tgtggtcggg caggatggcg aggatgacca aatcgcgcgg gccgtgcgcg
     6541 ccgtaagcaa gcgtcagttg gatgtctgcg gttttggacg ggccggaaat caggaataca
     6601 ttggtcggca taccgttttc caccagtttt tcgccttcga cggcattatg aaactcgttg
     6661 tacatcttgg acgtatcgaa caggcagaaa tgcacgggag gaacgaggct taaagtacgc
     6721 ggctcttcgg ggctggaaaa cagcatcagc gtgccggtgc gggcgatgcc gcattgcgcg
     6781 ccgctgaagc ccgcatcgat gttcgtgaaa aactcggttt tccaagtatc gatttcgcgc
     6841 tcgaaggcaa tcggttcgat attgctgtcc gccaatgcgg cacgggcaat ttgtccgtgt
     6901 tcggtcgcca agggcagcag gatgtttttc aaacccttgc cttctgccgc ttcgcggaaa
     6961 acctgcatcc aattgctttt cgtcacccaa taaatttcgg ttttgaccgc acgcatagcg
     7021 gcagcccaat gtttcagacg ctcaacttcg ctgccccaag aaacacccat ttcacggtaa
     7081 taatcaaaaa ccgcaggttc ttccatcggc aatgcgtcgg cttttttcag ttttgccaaa
     7141 atattttcac gcgcgctcat gctttgcctc cggtgcgttc caacaagaag gatgcgatat
     7201 gtttcggacg cggcatatcc ggctcgtcct tggcgatttt gccgccgatg ttcatcatac
     7261 agccgcagtc cgcgctgatg atttcggttg cgccggtttc tttcagcgcg gcgactttgt
     7321 ctgttaccat tgcgccggaa atatcggctt gtttgacgga gaatgtgccg ccgaagccgc
     7381 aacattcgct ttcgtggtcg tggacgatgc gttcgacgtt ttccataccg tcaatcagtt
     7441 gccagcctga aagatggaca ttcatttcgc ggcgggcggc gcaggaagtg tgaacggcga
     7501 ctttgagcgg ttcgcccttg tcttcgggtt tgaaaccgat ggcaagcagg aaatgggtaa
     7561 actcgatgat gcggccggcg caatccacag ccctttcctc gtactcgctg cctttaaaca
     7621 gcgtcggcca gtggtgtttc atcatgccgc cgcacgagcc ggacggcacg acgatcggcc
     7681 agttttcagg gaaaaggtcg agttgtgctt tggcgacatc gaaggcttcg gtcggatggc
     7741 cggatgaata ggcaggctgg ccgcagcagc tttgcgccat cgggaaatgg acgcgtatgc
     7801 cctgctgctc gattagggta atggcatcca tgcctgcttc gggcataaaa agatcaagga
     7861 cgcaagtgcc gaaaaaatag acatcggtcg gattgctgtc gtatcggatg atttttgggg
     7921 gaatggttgc gctcatgttt tttgataacg ggaaacccgt ttttctctgt agaaaggtaa
     7981 gcgtttactt taagtaattg actgttgcgg gtcaagtcta attttaaaaa ataatccggt
     8041 ttttcttaca aactgccccc ataacgctta ctgtacctta atctgatggt tttcgataat
     8101 aattatcatt acaatgcaat gccggttcgt ttgcttgtga acattcaaga tgccgactct
     8161 gacggcattc agacagcatc tgaaaacaat aacggcacaa ggatggggta caatcgccct
     8221 atggaaaaaa acaccctatc cgcccgcgca ccatcccctt ggctgccgct tctgctggca
     8281 attgccattt ttatgcagat gttggatgcg accattttaa ataccgcact gcctgaaatt
     8341 gccgccgacc tgaatgagtc gcctctggat atgcaactgg cagttatttc ctacacgctg
     8401 acggttgccc tgctgattcc tttgagcggt tatttggcgg acaggttcgg aacgaaaaaa
     8461 gtctttttcg gttcgattgc cgtttttatg ctcggatcgg cattgtgcgc cgcatcgggt
     8521 tcgctgtttg aattgacgct ttcccgtgtc gttcagggca tcggcggttc gatgctggtt
     8581 ccgataccgc gtctgaccat cttgcgtgtg tacgacaagt ccaagctgct caatgccatc
     8641 aattatgcgg ttatgcccgc attaatcggg ccggttttag ggcctttggc gggcggttat
     8701 ttggtcgaat acgcttcgtg gcactggatt ttcctgctca acctgcccat cggtctgctg
     8761 ggtttcatat tgggacgcaa catcatgccc gatattaaag gcagtaatat ctctttagac
     8821 ttcaaaggtt atctgatttt ttctgccgcc gcgtgcctct tgttactttc ggcagaaagc
     8881 ctgtcgcacg cgctgcctcc gtattttgca ctgttgccgc tgtgcggcgg actgctgttt
     8941 gcacgccgtt atttccgaca tatgaaaacc gcgtccaaac cgatttattc cgccgacctg
     9001 tttctgatac gcactttccg tctgggactg gcgggcaatc tgttcagccg tctcggcatc
     9061 agctcgattc cttttctgat gcccctgatg tttcaaatcg ctttcggctt cggcgcaagc
     9121 ctgtcgggtt ggctggtcgc acccgtcgcc ctgtcttcgc tgctggtcaa accgctgatt
     9181 gcaccgctca tgaaacgttt cggctaccgc acggtactgc tttggaacac caagctgctt
     9241 gccgccttca tcatgctgct cgccctgcct gacggaaact cgccgctgtg gatttgggtt
     9301 ttcctctcgc tggcgatcgg cgcgtgcaac tccctacagt tttctgccat gaacacactg
     9361 accctcgccg atttgcgccc gcaacaaaca ggcagcggca acagcctgat ggcggtcaac
     9421 caacagcttg ccatcagcat gggcattgtt gccggcgcat taatccttaa aaactggaca
     9481 tttctgatac cggcttcttc aggtctgcat tccgccttcc gtatgaccct gctcagcatc
     9541 ggcggcatca cccttgcatc atcgctggtt ttcaaacggc tgcacgtttc agacggcacc
     9601 aacctgacac ggaacacacc gtcctgaagc ggtccacacg caaaactttt acccgtttca
     9661 acgtttggat tatgataccg cacttccatg cgcgccaacc ccaaaacaca ggcaatgccg
     9721 tctgaaacca tatccctgat gaaaacacgc agcctaattt cccttttatg cctccttctc
     9781 tgttcatgtt cttcatggtt gcccccactg gaagaacgga cggaaagccg tcatttcaat
     9841 acttccaaac ccgtccgcct ggacaacatc ctgcaaatcc ggcacacccc tcataccaac
     9901 gggctatccg atatctatct gttgaacgac ccccacgaag cctttgccgc ccgcgccgcc
     9961 cttatcgaat ctgccgaaca cagcctcgat ttgcaatact acatctggcg caacgacatt
    10021 tccggcaggc tgctgttcaa cctcgtgtac cttgccgcag aacgcggtgt gcgcgtacgc
    10081 ctgctgttgg acgacaacaa cacgcgcgga ttggacgacc tcctgcttgc cctcgacagc
    10141 catcccaata tcgaagtgcg cctgttcaac cccttcgtct tacgaaaatg gcgcgcactc
    10201 ggctacctga ccgacttccc ccgcctcaac cgccgcatgc acaacaaatc ctttaccgcc
    10261 gacaaccgcg ccaccatact cggcggacgc aatatcggcg acgaatactt caaagtcggt
    10321 gaggacaccg ttttcgccga cctagacatc ctcgccaccg gcagcgtcgt cggcgaagta
    10381 tcgcacgact tcgaccgcta ctgggcaagc cattccgccc acaacgccac gcgcatcatc
    10441 cgcagcggca acatcggcaa gggtcttcaa gcactcggat acaacgacga aacgtccaga
    10501 cacgcgctcc tgcgctaccg cgaaaccgtc gaacagtcgc ccctctacca aaaaatacag
    10561 gcaggacgca tcgactggca gagcgtccaa acccgcctca tcagcgacga ccctgcaaaa
    10621 ggactcgacc gcgaccgccg caaaccgccg attgccgggc ggctgcaaga cgcgctcaaa
    10681 cagcccgaaa aaagcgtcta tctggtttca ccctatttcg tccccacaaa atccggcaca
    10741 gacgcactgg caaaactggt gcaggacggc atagacgtta ccgtcctgac caactcgcta
    10801 caggcgaccg acgttgccgc cgtccattcc ggctatgtca aataccgaaa accgctgctc
    10861 aaagccggca tcaaactcta cgagctgcaa cccaaccatg ccgtccctgc cacaaaagac
    10921 aaaggcctga ccggcagctc tgtaaccagc ctgcacgcca aaaccttcat tgtggacggc
    10981 aaacgcatct tcatcggttc gttcaacctc gacccccgtt ccgcgcgtct caacaccgaa
    11041 atgggcgttg ttatcgaaag ccccaaaatc gcagaacaga tggagcgcac ccttgccgat
    11101 accacccccg aatacgccta ccgcgttacc ctcgacaggc acaaccgcct gcaatggcac
    11161 gatcccgccc cccgaaaaac ctacccgaac gaacccgaag ccaaactttg gaaacgcatc
    11221 gccgcaaaaa tcctatccct gctgcccata gaaagtttat tatagaaata tagcggatta
    11281 acaaaaacca gtacg
//
LOCUS       DAWHRA010000066        10985 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWHRA010000066 DAWHRA010000000
VERSION     DAWHRA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10985
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.066"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1022..1792)
                     /gene="xth"
                     /locus_tag="WIB30_001246"
     CDS             complement(1022..1792)
                     /gene="xth"
                     /locus_tag="WIB30_001246"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ3797317.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1846..2133)
                     /locus_tag="WIB30_001247"
     CDS             complement(1846..2133)
                     /locus_tag="WIB30_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ3797318.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            complement(2538..3419)
                     /gene="nadC"
                     /locus_tag="WIB30_001248"
     CDS             complement(2538..3419)
                     /gene="nadC"
                     /locus_tag="WIB30_001248"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ3797319.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3494..4432)
                     /locus_tag="WIB30_001249"
     CDS             complement(3494..4432)
                     /locus_tag="WIB30_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797320.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGMLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVGRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            4634..5746
                     /gene="nadA"
                     /locus_tag="WIB30_001250"
     CDS             4634..5746
                     /gene="nadA"
                     /locus_tag="WIB30_001250"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ3797321.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYICRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKQFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA"
     gene            5904..6233
                     /locus_tag="WIB30_001251"
     CDS             5904..6233
                     /locus_tag="WIB30_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEZ3797322.1"
                     /translation="MHWLFLTVAILSEVCGSSMLKLSGGFSKLWPSIGVVVSFSVCFW
                     ALSMTLKTMPLATAYAIWAGVGLVLTALVSVVFFGEKADFIGIVSIGLILLGVVLLNT
                     MSHMSGH"
     gene            6282..7790
                     /gene="nadB"
                     /locus_tag="WIB30_001252"
     CDS             6282..7790
                     /gene="nadB"
                     /locus_tag="WIB30_001252"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ3797323.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRAILSQGKPAIEWLLAQGVAFDR
                     NHNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLDCRTQETHRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VGNLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLCAQA
                     IADGQAFQAEPFQRPSESLSAEAGIFSDDLQNTFSRPVLQTFNQRHLGILRNDTGLRR
                     AIAQLQLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(7968..8633)
                     /gene="pgmB"
                     /locus_tag="WIB30_001253"
     CDS             complement(7968..8633)
                     /gene="pgmB"
                     /locus_tag="WIB30_001253"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ3797324.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRYKNDNYVEMIQAVKPEDVYPGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTDFFDAIADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSGIAELTYAYLQNVWAQ
                     SGR"
     gene            complement(8646..10904)
                     /locus_tag="WIB30_001254"
     CDS             complement(8646..10904)
                     /locus_tag="WIB30_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ3797325.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRLFTVFGVRFDVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDGLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGRQTDWQVSNSFEAEVGGTPKTFEKRVIVTTSRDYQGLEAVKVAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRRGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTVALEK"
BASE COUNT         2409 a         2898 c         3067 g         2611 t
ORIGIN      
        1 tgaaactgat gcagatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
       61 cagtacaaat agtacggcaa cgctgtactg gtttaaattt aatccactat attttgtaat
      121 agctgccatt atgcctacta ggatatatac cttctatata atccttattt aaataaaaac
      181 ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa gattttatct ttctcatcag
      241 gtttatattt gctatcaata tcaaaaagag aataaggatt acaatgagtt cgacatacta
      301 ggtaaaaatt tatatcttgg atcatgaaat attcgatatt tatcaggatc gaaagatgct
      361 aaaaattcat caatatcggt actggatatt ttgtcatcac taataaacat cgctagagat
      421 gggcgacttt gattttccat ttgttgatta gtaaaggata taaatactaa cttgccattg
      481 tcaaaccaca tagatttata tagtggatta aatttaaacc agtacgtcgt tgcctcgcct
      541 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      601 tatagttatt gcctttggga gaatcaattc tgacagcaca taacataggt tcatctttac
      661 agtcgttata ttgggtttca tcaaactcaa ctgcatagca caataaaggc aataggaaaa
      721 aaaggagatt aatttattac tatacataag gcagcctgac tattcattct aaaataaatc
      781 cggtttttaa tatttgattc tgctccggat tatatacata ataaatttta ctgctccaaa
      841 atctgatatt aaccctatgg atttcataaa tttttcagaa aaataggctg tgcttgaaca
      901 atccgccgac tccggctgtt tgtcagggaa aaggcaatta ttttcggtta gtcagcagcc
      961 tatcctattg aatcacttaa agatatggat gtttgtcaac gccatatcaa aacacggtct
     1021 tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct cggacgctcc agcgcgcgcg
     1081 tctccaaatc aacgcggaca tccttcaacg ccgccgccat cgcaggcgac accaaaatat
     1141 ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc gcgatagtcg aaccaggtat
     1201 agaacgcgcc ttcgggatgg acttggcgca ggctgtcggt cagtcccaaa tccagcaggt
     1261 ttttgaacca ctgccgttcg acggacgaac agtggatttt ttcgtgccat ttttcagggt
     1321 cgtaacagtc cgcatcggca ggcgcgatat tgaaatcgcc cagtagcacc agtttgccgt
     1381 agcgggtcat ttcatcgcgg acaaattcgg ttaacgcggc aaaccattgt tctttatatt
     1441 tgaatttcgg gctgtcgagg gcttcgccgt tgacgcaata gacattgatg acgcgcacac
     1501 cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc cggcagcgcg ggcagcccga
     1561 tatgcacgtc ttccggcgcg ttgcggctga cgattgccac gccgttgtag gttttctgcc
     1621 cgctccaaac acagtgccag cccatcattt gcaaagcggc ggccggaaat ttgtcctgat
     1681 cgagtttgag ttcctgcaaa accaaaatat cgggcggatt gtcggcaagc aggttttgca
     1741 cctgcggcag ccgcacattg agcgaattga cgttccaagt ggtgattttc ataacatttc
     1801 cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa ggattttaac accataccgc
     1861 gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt gccgcgtctt cggaaatcag
     1921 gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc agaccgcccg ccctcaggcg
     1981 gttcaaatgg ccggaaacgg cggtggccgg cagggataag gattttgcca gttcggcgat
     2041 attgcgttcg ctgtccaaca gttggatcag tatcgccata cgttcgggat gcgccataag
     2101 tttgagtatg gtgtggagcg gtattgtgtt catcggtatt cctaaaacct ttgccgcccg
     2161 ccaagcggat tgacgggcga cagatatgga tgattgtgat tatctttata aatcgtatta
     2221 taaatcaaat atttatggat gattgtacag gattgtatgt tccttttccc acttttttgc
     2281 aaacacgcgt aaaggcgggc tttcagacgg cataaaactg ccgcccccgt cctttcccct
     2341 gccctaccga tgcctccgcg ctgtcgggca aacaaaatac ctgcctgaaa cgctgttttt
     2401 tctaaagcat cacgaccttc acgaaaaatg ccgtctgaag aatatttcag acggcatttc
     2461 ctccttgagg caataaccgg attacggtta ggcggttcct tgtctgacat atcagacaac
     2521 tgccgacacc ctaaaactca tgccaagaaa tccaaaccta tgtccaacga gcggctgctg
     2581 tgggtcagat agccgagggc gatgccgtcc acgccggttt gcgccacgcg cttcaggcgg
     2641 tcgaagccga tgccgcccga tgcttcgcaa tagatggtgt gggggtgggc ggtttgcgtg
     2701 tggcagcggt ttgccgcttc tttcagggtt tcgtcgtcca tgttatccag caaaatccgt
     2761 tccgcgcccg ctgcgatggc ttcgtccagt tgtgccaacg tatccacttc gatttccacg
     2821 caggtcaatg ctccgacagc ctgttttgcc tgctgcacgg cttgggcgat gctgccgcaa
     2881 taggcgaggt ggttgtcttt gatgagcacg gcgtcgtcca aacccatgcg gtggttcaca
     2941 ccgccgcctg ccctgacggc gtatttttgc aggacacgca gcagggggat ggttttgcgg
     3001 ctgcacacga tgtctgtacc gtattcggcg acttcggcaa cggcacgcgc ggtggcggtg
     3061 gcgatgccgc ttaagtgcgt gaggtagttg agcgcggtgc gttcggcggc gagcagcgcg
     3121 cgggcgttgc cttcgacggc ggcaagcgtc tgacctgcgc ggacggcttg cccgtctcgg
     3181 atttcggctt ggaagcggac ggacggatcc atcgtctgaa aggcgagacg cgccaagtcc
     3241 atgccggcga taacgccgtc ttcgcggctg acaaggaaga gtttggcggt tttgtcgggg
     3301 gcgatgacgg cggcggacgt aatatcgccg cgcctgccca agtcttcgct caaggcttgt
     3361 tccaccatgg ggcgcaacag ggtgtcgggc agggggaaga gggtgttttc agacggcatg
     3421 gggtttcctt tgttgaaaaa gattgatgtt gcaccgatgg gcaaaacttc gtttgatgtc
     3481 gtctgaaaac gggctaacgg ctgcccagcg gcagtccgat gtccgaaatc agcctgtcgg
     3541 gcaggacgtc gtcgcggaag cggcaaagct gcgcgggacg gcctttgctg cccgatacac
     3601 cggtatccga cggctcgatg aggttttgct gctgaatctg gcggcggaag ttttgcttgt
     3661 gcagcaatct gccgctgatg gcttcgacac tgttttgcag ttgcagcagc gtgaattcgg
     3721 gcggcatcag ttcaaaaatc acggggcggt atttgatttt ggcgcgcagg cgagacaggg
     3781 cggtcgccag tacgcggcgg tggtcgtggc gcatgggctg ccccgtaagc gcgaagtcga
     3841 agtttgcctg cggctcggcg gcctccgcta tcaggccgct ttcatacagc atttcatagc
     3901 gttgcaaaac gtattcttcc gaccagtttt ccggttcgac cccccaacac aaatgaatgc
     3961 gcttgagccg cttttggcgc acttcctccg tgtccgccga gtttgcccaa atgcgcaggc
     4021 ggccgacgac ggcgtcgcgc tgcccgccgt cggtacgcaa atcttcccac gggaaatagc
     4081 cgtagcagtc ctgccatttc gcgtcgggat gcaggatgct gtcggctgcc tcgcgcacca
     4141 gccccaaata gctgacgtac aagacgggca tgccgtgttc gttgcggcgg tgggtatcga
     4201 caaaggtgta aagctgttcc acatagccca taggctgcga agtctgcttg gcgacccaca
     4261 gttttacgcc tgcctgcaag gaattgcgca ggggggagag cgggccgttg ggcaggagca
     4321 tgccttgggc gacggtcaat acccgcaggc cgccgtcggt aacggcaatc aatacgggaa
     4381 ccagctcgac gatgctttgc ggcggggctt cggcttcggg gtaggcatcc atgctcggct
     4441 ttctgcgggt gggaaaggct ttattgtaaa ccaaagcggt ttgtcggaac gaagcgggtc
     4501 ggttttacgg tattgcaaac ctgttttcag acgaccttgc atattatact caagttgagc
     4561 ataatgtaaa aacagtttga aaaacagacg gcaaaccttc atgccgtctg aaaaacagct
     4621 acaaggaaac atcatgcaaa ccgccgcccg ccgctcgttc gactacgata tgccactcat
     4681 ccaaacgccg acttccgcct gccaaatccg tcaggcgtgg gcgaaggttg ccgacacgcc
     4741 cgaccgcgag acggcagatc gtctgaaaga cgaaatcaag gctttgctga aggagaaaaa
     4801 cgcggtcttg gtggcgcatt attacgttga tccgctgatt caggatttgg ctttggagac
     4861 gggcggatgc gtgggcgatt cgctggaaat ggcgcgcttc ggtgcggaac acgaagccgg
     4921 tacgctggtg gtggctggtg tgcgcttcat gggcgagagc gcgaaaatcc tctgccctga
     4981 aaaaacggtg ctgatgcctg atttggaggc ggaatgttct ttggatttgg gttgcccgga
     5041 agaagcgttt tcagcgtttt gcgaccaaca ccccgaccgc acggtggtgg tgtacgccaa
     5101 cacttccgcc gccgtgaaag cgcgtgccga ttgggtggta acgtcttcgg tggcgttaga
     5161 aatcgtatcg tatttgaaat cacgcggcga gaagctgatt tggggacccg accgccacct
     5221 cggcgactac atctgccgcg aaacgggcgc ggatatgctg ttgtggcagg gttcgtgcat
     5281 cgttcacaac gaattcaaag ggcaagagct ggcggcgttg aaggcggaac accccgaagc
     5341 ggtggtgctg gttcatcctg aatcgccgca aagcgtcatc gaactgggcg acgtggtcgg
     5401 ctcgaccagc aaactgctca aagccgccgt atcgcgtcct gaaaaaaaat tcatcgtggc
     5461 gaccgatttg ggcatcctgc acgaaatgca aaagcaggcg cccgacaaac aatttatcgc
     5521 cgcgccgacg gcgggcaacg gtggaagctg taaaagctgc gcgttctgcc cgtggatggc
     5581 gatgaattcg ctgggcggca tcaaatacgc cctgacaagc ggacgtaacg aaatcctgtt
     5641 ggacaggaag ctgggcgaag ccgccaaact gcctttgcag cgtatgctcg actttgcggc
     5701 agggctgaag aaaaaagatg tgttcaacgg catgggcccc gcctgatttg tcgttcgtaa
     5761 tgggtgcagc ctgaaaatac cgcccgcctg ctttcccaag acgggatttg cacacaaaaa
     5821 atctttaaca acaagactgt ttttttattt tattgcattg atataattat actttctttc
     5881 atttatcagg taaaaacatg caaatgcact ggctctttct gactgtagca attttaagcg
     5941 aagtctgcgg ttcttccatg ctcaaactga gtggcgggtt tagcaaactg tggccttcta
     6001 ttggcgtggt agtcagcttt tcggtgtgtt tttgggcctt gtctatgaca ctgaaaacca
     6061 tgccgctggc tacagcatac gccatttggg caggcgtggg actggtttta acggctttag
     6121 tcagcgtggt gtttttcggt gagaaagctg atttcattgg gattgtcagc atcggtttga
     6181 ttttgctggg cgtggtgctt ctcaatacca tgtcgcatat gtcggggcat tgattaatcc
     6241 actataccgt ttcagacggc ctctcaaaca aggacaacac catgcaaacc gattgcgacg
     6301 tattgattgc cggaaacggg ctggcggcac tgacgctcgc cctgtcgctg cctgaatcgt
     6361 tccgcatcgt tattttgtgc aaaaaccggc tggacgacac cgccagccgt catgcgcaag
     6421 gcgggattgc ggcggcgtgg tcgggagagg acgacatcga aaaacacgtt gccgatactt
     6481 tggaagcggg cgcgggtttg tgtgatgaag ccgccgtccg cgccatcctg tcgcagggca
     6541 aaccggcaat cgaatggctg ctggcgcagg gcgtggcgtt cgaccggaat cataacggcc
     6601 tgcacctgac gcgtgaaggc gggcatacct gccgacgaat cgcccacgtc gccgactaca
     6661 cgggcgaagc cgtcatgcag agcctgattg cccaaatacg ccgccgcccg aacatccgcg
     6721 tttgcgagcg gcagatggcg ttggacattc aaaccgaatc aggcgcggcg tgcggactga
     6781 ccgtcctcga ctgccgaaca caagaaaccc accgcatccg cgcccgccat accgtactcg
     6841 caggcggcgg cttgggacag atttacgccg ccaccaccac gccgcccgaa tgcacgggcg
     6901 acgccatcgc catggcgata cgcgcaggct gcgcagttgg aaacctcgaa tttatccaat
     6961 tccaccccac aggcttggca agaccgtctg aaaacggacg caccttcctc atttccgaag
     7021 ccgtgcgcgg cgaaggcggc atcctgacca accaagcggg cgaacgattt atgccgcatt
     7081 acgaccgccg cgccgaactc gcaccgcgcg acatcgttgc ccgcgccatc gcagccgaaa
     7141 tcgccaaaca aacgcaagac ttcgtctcgc tcgacatcag ccatcaaccc gcagcgttcg
     7201 tccgccggca tttcccatcc atccatcgac actgtttatc ccaatgcggc ctagacatca
     7261 cgcgccaagc catccccgtc cgccccgtgc aacactacac ctgcggcggc atccaaaccg
     7321 acccctgcgg cagaacctcc ctgccgcagc tctacgcctt gggcgaaacc gcctgcactg
     7381 ggctgcacgg agccaaccgc cttgccagca actccctgct cgaatgcgtc gtgaccgcaa
     7441 ggctctgcgc ccaagccatc gcagacggac aagcattcca agccgaacca ttccaaaggc
     7501 cgtctgaaag cctctccgcc gaagcaggca tcttttcaga cgaccttcaa aacacattca
     7561 gccgccccgt cctgcaaacg ttcaaccaac gccatctagg catcctacgc aacgataccg
     7621 gcctgcgccg cgccatcgcc caactgcagc tttggaagca aaaccaagcc gaaccgcaca
     7681 ccgcgtccga atacgaaaac cgcaacctgc tcgaatgcag cctcgccgtc gcccaagccg
     7741 catacaggcg gagacagaac atcggcgcgc attttaatag tgattgttaa ggttggctgg
     7801 ttagaaaaaa aatcgttcaa ataaatttga atagcatggt taagagattg gcatttagtt
     7861 tttagggttg tgtaagttgg gttatcattc tgttctaaaa gcagaaaggc cgtctgaaac
     7921 actgttcaga cggcctttta cttccttgac actttatctg acgcgtttta cctgcccgac
     7981 tgtgcccaca cgttttgcag gtaggcgtag gtcagctcgg cgatgccgga caccagcgcg
     8041 atgtcgctgc ccaagtcctc ggctttgccc acgccgatgg gcaaggcgcc ggcggctttg
     8101 atggcggcta cacctgcggc ggcgtcttca atgccgatgc agcggcggat gtctgcgccc
     8161 acgccctcgg cggcggcgag gaagatgtcg ggggcgggtt tggaatgcgc gacggcggca
     8221 gggtcggcaa tggcgtcgaa gaagtcggtc agccccatgc gttcgagcag gaaggggccg
     8281 tttttactgg cggacgcgag ggcgattttt ttgccgtttg ctttcaatgc ttccagcagc
     8341 ggcaaaatgc cggggtacac gtcttcgggt ttgactgcct gaatcatctc gacgtagttg
     8401 tcgtttttgt agcgggtcag ttcggcaaac tcggcttcgc cgacggtttt gccgccgtgc
     8461 gcgaggatgc gtttgagcga atcgtcgcgc gacacgcctt tgagctgctc gttaaacttg
     8521 cggtctatgc cgatgcccag ttcttcggcg agctttttcc atgcgcggta atggtattcg
     8581 gcggtatcgg tgatgacacc gtcgagatcg aagagcacag cggtaaacgt cattttgcgc
     8641 cctccttatt tttccaacgc aacggtgtgg ctgccgttga gcgtgatgtc tttgccgtac
     8701 acctgcaaat caagcggttc gcctttgagc agggtgaaga cgacgttttc tttgccgacg
     8761 gcgactttaa tcagacggcc tcggtagttg atatggaagg cgtagcctgt ccatgcgccc
     8821 ggcaggaacg gtgcgaagct gagtttgccg ccccaagttt tcatttgggc gaaaccttgg
     8881 acgatggcga gccacgagcc ggtcatagaa gtaatgtgca ggccgtcttc ggtgtcgttg
     8941 ttgtagttgt ccaagtccag gcgggctgtg cgccggtaca tttccacggc tttttcttct
     9001 ttgcccagtt cggcggcgag aatagagtgg atacacggcg acaacgagct ttcgtgcacg
     9061 gtcatcggtt cgtagaagtc gaaattgcgg cgtttttcgt cgatattgaa acggtcgccg
     9121 aagaaataga tgccttgcaa tacgtccgcc tgtttgataa agggcgaacg caggattttg
     9181 tcccacgacc atttctggtt gagcggcaaa tcgtcgggcg aaagcgcgga cacggggcgg
     9241 atgtctttgt cgaggaagcc gtcgtgttgc acgaatacgc cgagttcttc gtcatgcgga
     9301 cggtacatat tcgcgctgat gtccgcccat ttttccaact cggcggcacg cacgttcaaa
     9361 tccggacgcg ggtatttcgc caaggcttcg cgggtgtagt ccaataccca agcggcaagg
     9421 gtgttggtgt accagttgtt gttgatgttg ttttcgtatt cgttcgggcc ggttacgccg
     9481 tgaatcatgt atttacccct gcgtttggag aagtggacgc ggtccgccca gaagcgggac
     9541 acttcgacca agacttccaa gccttctttg gcaagatagc tttcgtcgcc ggtgtagttg
     9601 gtgtagttgt agatggcgta aggaatcgcg ccgttgcggt ggatttcctc gaaggtgatt
     9661 tcccattcgt tgtggcactc gatgcccgta aacgttacca tcggatagag tgcgcccgcc
     9721 aagccctgtt cgcgcgcgtt gtgctgcgcc tgcggcagtt ggttgcggcg gtattgcagc
     9781 aggttgcggg taacttcggg ttccgccagc gcgaggtaga gcggcactgc gtaggcttcg
     9841 gtgtcccaat aggtcgcgcc gccgtatttt tcgccggtaa agcctttcgg gccgatgttc
     9901 aggcgcgcgt cttcgccgta gtaggtggag aacagttgga acagattgaa acggatgcct
     9961 tgttgcgctt cgtcgctgcc ttcgatgacc acgtcggcga tttcccaacg gtgcagccag
    10021 cctgctttgt gcgcgtccag caaggtttca aacgcaacgc ctgcgacttt ttccgacaag
    10081 gcacgaccgg cgactttcac tgcttccaag ccctgataat cgcggctggt ggtaacaatc
    10141 acgcgttttt caaaggtttt gggcgtgccg ccgacttcgg cctcgaaaga attggagacc
    10201 tgccagtcgg tttgtctgcc gccgagggct ttgaagctac cggcgaaagt ttgctcggcg
    10261 ttgacgataa attgttccac accaaaaggg ttggcaacgg tttgggcggc aatgtaggag
    10321 aggccgtctg aaacgccttt gtccaatacc tgccagaatt tttcttcgta gttggagtct
    10381 tcgtttttca cgtcggcatc gatgatggaa tcgatgcgga cttggtgggt tttgccgtca
    10441 acggatacgg cttcccagcg gataaccgcc agctcttttt gcgcgacgga caggaatttg
    10501 cacacatcga aacgcacgcc gaatacggtg aacaagcggc gcaacacgcc gtgctgcata
    10561 tcgagttcga cggagaagcc ggcaacatcg tttttcgcca agtccacctc ttgaccatcg
    10621 acaaagattt tgactttgct gaaattgagc gcgttgatgg ctttgccgaa atatttggga
    10681 tagccgtttt tccaccagcc gacgcgggtt ttgtcgggga accacacgcc ggcgatgtag
    10741 gtgcccaagt ggctgtcggc ggagtaggtt tcctcaaagc tgccgcgcat acccatatag
    10801 ccgttgccca aactggtcag gctctcttgc agccgtttgt gttctttttc cagttttgcc
    10861 gaacgcagcg tccaagggct gatttccata attcttgtgt acatttatga agctcctgtt
    10921 tggattgatt tgagggaatg gtgaaatctt atagtggatt aacaaaaatc aggacaaggc
    10981 gacga
//
LOCUS       DAWHRA010000067        10924 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWHRA010000067 DAWHRA010000000
VERSION     DAWHRA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.067"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(5..1006)
                     /locus_tag="WIB30_001255"
     CDS             complement(5..1006)
                     /locus_tag="WIB30_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ3797326.1"
                     /translation="MIDRQTNEPKQKNRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRERLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEAYEPPAHWEWIRKIRDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPLARLKQWLGMMKKEFAAAQNLFDRVRTVKDADEVRNILAEFEREMNT"
     gene            complement(1187..5020)
                     /locus_tag="WIB30_001256"
     CDS             complement(1187..5020)
                     /locus_tag="WIB30_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ3797327.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKTRWEWLLANLDTPLADYKARYGAA
                     VHAAPEAKNNESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLIEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQIGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKYILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5281..5377
                     /gene="ffs"
                     /locus_tag="WIB30_001257"
     ncRNA           5281..5377
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WIB30_001257"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5544..7235
                     /gene="dld"
                     /locus_tag="WIB30_001258"
     CDS             5544..7235
                     /gene="dld"
                     /locus_tag="WIB30_001258"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ3797328.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKVMQFVSKFLPDHLPKSMRDYRNKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7367..7474
                     /locus_tag="WIB30_001259"
                     /pseudo
     CDS             7367..7474
                     /locus_tag="WIB30_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7530..7901
                     /locus_tag="WIB30_001260"
     CDS             7530..7901
                     /locus_tag="WIB30_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ3797329.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8056..8604
                     /locus_tag="WIB30_001261"
     CDS             8056..8604
                     /locus_tag="WIB30_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ3797330.1"
                     /translation="MARLTVHTLETAPEAAKACVEAVLQNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLAQTEINPELQAYA"
     gene            8740..9831
                     /locus_tag="WIB30_001262"
     CDS             8740..9831
                     /locus_tag="WIB30_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ3797331.1"
                     /translation="MNAQTLLTNVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGKVLAGTGMSNTVKHLVGIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQREGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNSYLDDGYDSLKARLD
                     GAWAETERLADLAWDGAPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10467..10625
                     /locus_tag="WIB30_001263"
     CDS             10467..10625
                     /locus_tag="WIB30_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ3797332.1"
                     /translation="MCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGVGKED
                     FYLLDFVI"
BASE COUNT         2571 a         3018 c         2914 g         2421 t
ORIGIN      
        1 atattcaagt attcatttct cgctcaaatt cagccaagat gttccgaact tcgtccgcat
       61 ccttaaccgt tcggacgcgg tcgaacagat tttgtgctgc tgcaaattct ttcttcatca
      121 tacccagcca ctgtttcagc cgcgccagcg gatatttgtt gtttgcctct tttgtcaggc
      181 acagctcgaa aaactgccgt atccatttgg aaacttcggc aaaatccgtg tctttgaccg
      241 gcccgccgtt ctcgtattgc ttgatttgcc gcgccaaatc ggggcggatg accgcgccgc
      301 gaccgagcat cacgctgttg cagccgctga ttgttttgat gccgatatag tcctgcaggc
      361 tgaaaacgtc gccgttggcg gtaacgggaa tattgacggt atcgcggatt ttgcgtatcc
      421 attcccaatg cgccggcggt tcgtaagcct cggctttggt acgcgcgtgt acggtcagtc
      481 cgcatgcgcc cccttcggca atcgcacagg cgcattccaa agcccggctt ttgtcttcgt
      541 aaccgagccg catttttgcg gtgagcggaa tatgtgcggg caaacgttcg cgcagcgttt
      601 tgacgatgtg gaatatcagt tccggctctt ttaaaaggat tgcgccgcct ttgtgtttgt
      661 tgacggtggg cgcggggcag ccgaagttca aatcgatttt gttcgcaccg aagcggacgg
      721 cttccagcgc attcgccgcc atattgtccg catcgctgcc caaaagttgg acggtgcagg
      781 gcgtgccgga aaacgttttg tttccgttgg cgatttcggg gacatatttt aaccatatgg
      841 atcgggaatg cacggtatgg gtaatgcgta caaattcgct gacgcattcg tcgtagccgc
      901 caatacgcgt cagcaggtcg cgcatcacgt catcgaccag accctgcata ggggcaagga
      961 tgattcggtt tttttgtttg ggttcgtttg tttgtctgtc aatcatgatt tgggtaggta
     1021 tagtggatta aatttaaacc agtacagcgt tgcctcgcct tgccgtacta tttgtactgt
     1081 ctgcggcttc gtcgccttgt tctgattttt gttaatccac tatatttggt tcaaacaatg
     1141 ccgtctgaag gctttcggcg ttcagacggc atttccgtgt ccgttgttac agcaacactt
     1201 tctctacacc gccgttgttg gcttttttta caaactcatc cagccagttt tcgccgagga
     1261 tgtatttcgc catttcgatg acgatgtagt cggcaggcat attgttgtcg tcggcgtagc
     1321 ggctcaagcc ttgcaggcag gcggggcagg aggtcagcat tttgacgggt tcgccctgcg
     1381 gcagctcttt gaggtttttc tcgatttcct cttgtttgcg gaacttgacc tgagtggcga
     1441 tgtctggccg tttgacggca aacataccgg attcgccgca gcagcggtcg cttaaaacga
     1501 ctttctgccc catcaggctg ctggccattt gggtggcgtt catggttttg atgggggtat
     1561 ggcaggggtc gtggtagagg tattgctgac cttttacgcc gtcgagtttc acgccttttt
     1621 cgagcaggta ttcgtggatg tcgatgattc ggcagccggg gaagatttct tcaaagcggt
     1681 atttttcgag ctggtcgtaa caagtgccgc aactgacgac gacggttttg atgtcgaggt
     1741 agttgagggt gttcgccata cggtggaaag ccacgcggtt gttggtgctc atttcttcgg
     1801 cttttgcctt attgccgcct gcgtcttgcg gatagccgca acacatatag ccgggcggca
     1861 ggacggtttg tacgccgacg tgccagagca tggcttgaac ggcaagtccg atttggctga
     1921 acagacgctc agagccgcaa cccgggaagt agaacacggc ttcggcatct tcgggcgcgg
     1981 cggggttgcg gatgatgggg atgcttttgc cgtcttcgat gcccaataag gagcgcggtg
     2041 ttttggcggg tacgtttttg ggcagtgggc ggttgatgaa atggataacc tgttctttaa
     2101 tcggggcttt gccgacggtt gccttgggtt cggctttttg ctttttcgtg ccgattggaa
     2161 gaagtttgcc gattttgtag gcgaaattct gcgctgggaa gccgatctgt atcatggcgg
     2221 cgcgaagggc tttgatggtt ttcgggccgg tggcgttcaa aaacgccata cccatagctg
     2281 cggcaggcgc aaatcgtttg tggccggaat cggcaagata gttgcggacg gctacggtaa
     2341 cgtcgccgaa gtcgatgttg acggggcagg gtttgacgca gcggtggcac acggtgcagt
     2401 ggtcgccgat gtccatgagt tcttcaaagt gtttgatgga aacgccgcgg cgggtttgtt
     2461 cttcgtataa gaaggcttcg atcaataagc ccacgccgag gattttgttg cgggggctgt
     2521 acagcaggtt ggcacgcgga acgtgagtag agcagacggg tttgcatttg ccgcagcgca
     2581 ggcagtcttt gacggaatcg gcaatcgtgc cgaggtttga tttttccata atcagcgatt
     2641 ccgcgcccaa cagctcgaag gacggcgtgt aggcgttgcg taagtccgag cctttcatca
     2701 gtttgtgacg gttgaaggtg tgtttcggat cgacttggtt tttgtagtcc caaaacggct
     2761 gcatttcttc gtcgcttaaa aattcgagct tggtaatgcc gatgccgtgt tcgccggaaa
     2821 tcacgccgtt aagcgaacgg gcgattttca taatgcgttc gactgagcgg tatgccgtct
     2881 gaagcatttc ggcatcgtct gagttaaccg gaatattggt gtgaacgtta ccgtcgccgg
     2941 cgtgcatatg cagggcgaca aagacgcggc tgcgtacggt ttttgcgtgg atttttccca
     3001 agccttggat aattttggtg tcggttttgc cgctgaagat ttcagaaagc ggtttcatta
     3061 cgtccgcttt gacagacacg cgcaggcgga aatcgcggaa ggcaataaag cagctttcat
     3121 tgtttttggc ttcgggtgcg gcgtggacgg ctgcgccgta gcgggctttg tagtcggcaa
     3181 gcggcgtgtc aagattggcg agcagccatt cccaacgcgt tttgacggcg gaaacgtggg
     3241 ccagggcgtg tttgccgcgt tcgcccaaca gttcggcggt cggcaggtca gtgcccattt
     3301 tgtcgatggg gagtttgccc gaaagatatt gctccaaggc ggcacagagt ttgagcttgt
     3361 tttggatgga aagctcaatg ttgatgcgtt cgatgccgtc cgaatactcg ccgagccttt
     3421 cgagcgggat gaccacgtct tcgttgattt taaaggcgtt ggtatgtttg gcgatggcgg
     3481 cggtgcggct gcggtcgagc cagaaggttt tgcgggcttc gggcgatacg gcgataaagc
     3541 cttcgccgtc gcgggcgcgt gcgagttcac agatgtgttc ggcggctgcc tctacggcgg
     3601 cttcgtcgtc tgaaaccacg tctgccagca aaaccatttt cggtcgtccc ttgcccgccg
     3661 ctttggtggc gtagccgacg gcgcggacat aacgccagtc caaatgttcc aaacccgcca
     3721 gccgcacgct ttcatgggca agcaaaaagt cgcggatttc gacaatagat ggcgtggcgg
     3781 tggcgaccgt accgaaaaac tccatacaca cggtgcgcgt gtatttcggc attttatgca
     3841 acacgaaggc aacgctggtg atgatgccgt ctgtaccttc tttttgcacg ccgggcaggc
     3901 cgctcaagaa tttgtcggta acgtctttgc ccaaaccgac tttgcggaat ttgtggccgg
     3961 ggatttccaa gcgttcggtt ttaacgatat tgatgccgtc tgaatccagc gtgtgaacgt
     4021 cgaacacggc ggtttcttcg tcgtggattt tgccgaaatt gtggcgcacg cgctcgatac
     4081 gcagccattc gccttgaggg ttaaccatgt tccagtaggc gaggttgtcc aacgccgtcc
     4141 cccacagcac ggcttttttg ccgcccgcgt tcatcgccac attaccgccc acgcatgacg
     4201 cgtcggcaga agtcggatcg acggcgaaca ccaaacctgc ctgatgcgcg gtttcttcca
     4261 cccgccgcgt aaccacgccc gcgccgcacc ggataatcgg atgcttgccg tccaagcctg
     4321 ccagctcaac gtattcaaca ccacgatgct tgtcgagttt ttcggtattg atgactgcgc
     4381 tgtttgcgtc caaaggaatc gcgccgccgg tataacccgt gccgccgccg cgcggaataa
     4441 tgaccaaatc cagctcgatt aaggcgcgca ccaaaggcgc gatttcagcc tccgtgtcgg
     4501 gattgacgac gacaaacgga tactccacgc gccagtcggt tgcatcggta acgtgcgtta
     4561 cccgcgccag cccgtcgaac ataatattgt gcggcttggt gattttgctc aaacgctcca
     4621 aaatctgccg ccgtttttgg ctggtttcat caaaactgct atcaaaacgc tcgactgctt
     4681 tttctgctgc ggcaaccaac acatccactt gccgattatc gtcgcggcgt ttgcggattt
     4741 catttaagcg gtggcgcatt tcacgtacca gcgcggcgcg gcgttttggg tgctccagca
     4801 agtcatcgac cagatacgga ttgcgcacga ccacccaaat atcacccaac acttcaaaca
     4861 gcatccgcgc cgaacgcccg gttttgcgtt gaccgcgcag gtcttgcagg atttgccacg
     4921 cttcgtcgcc caataatcgg atgacgattt cgcggtcggt gtaggaagta tagttgtagg
     4981 ggatttcccg aatacgctga ggggcggtag tcgtggtcat ggtgtgtccg tgtttggcag
     5041 tttttatttg aatgccgtct gaaaagtcgg tttcaggcga gtttgaaaag gcgataatgt
     5101 agttcaattt ttgatacttt tcaatgccga aacaggaaat acccttatag aaacaaaata
     5161 acgctttgag aaattgttga caatattatc aaatataaat tttaggtggt gtttccggcg
     5221 tatgaccgta tcaaaacggt tgagtttttc agaaaacccg ttataatgcc gtttctctga
     5281 ggcgggtctc cccgcatggc aaatcggaac accgggtcag gggcggaagc cagcagccca
     5341 ctccaatgcg ccagtgccgg gggtttggcc cgccgcccta tttgaaacgc cggagcttcg
     5401 atgttccggc gttcccgtat cgcaccgatg ccgtctgaaa gccgttcaga cggcattttc
     5461 tttactttgc cataacgtat aactacaatg tgaaaacaat tccaatgtgc aatgcacctg
     5521 ccgacaattt taaggaaact gccatgagcg catcacaatt actgtcaaga ctgacccaaa
     5581 ccgtaggcga aaaatacatc atcaccgacc ccgcgaaaac cgaacaatac cgccaaggtt
     5641 accgcttcgg cgagggtaag gcgttggcgg tggttcgccc cggaagcatt ctggaaatgt
     5701 ggaaaattct gcaggcgtgc gtcgaagcag acgtgattgt gattacgcag gcggcgaata
     5761 ccggtttgac gggcggctcg acccccgacg gcaacgatta cgaccgcgac atcgtgattg
     5821 tgaacaccat gcggatgaac atcatccaaa ccatcaacaa caacgaacaa gtcgtctgcc
     5881 tgcccggctc gaccctgaac cagcttgaat tgctgctgaa acctttgggg cgcgaaccgc
     5941 attcggtcat cggctcatcc tgtatcggcg cgtccgtctt gggcggcgtg tgcaacaact
     6001 ctggcggcgc gttggtacag cgcggcccgg cctacaccga aatggcgttg ttcgcccaaa
     6061 tcaacgaaga aggcaggttg gaactggtca accacttggg catagacttg ggcgatacgc
     6121 cggaagaaat cctgaccaac ctgcaaggtc atcattatca aaacaaagac atcaaacaag
     6181 acgcgggcaa aggacacgac cacgcctatt gcgaacacgt ccgccaagtg gacgaaccga
     6241 ccgccgcgcg tttcaacgcc gaccccgcgc gccattacga agcctcgggc tgcgcgggca
     6301 aactgatggt gttcgccgtc cgtttggaca ccttcccgca agaaaaacaa accgccgtgt
     6361 tctacatcgg caccaacgac atcaacgagc tgaccgacat ccgccgcgcc gccttgggcg
     6421 aatttgaaag cctgcccgtt tccggcgaat acatccaccg ccacgctttc gatattgccg
     6481 acgtgtacgg caaagacacg ttttacgtca tcaaaaaatt cggtacgcac caactgccga
     6541 aattatttga tttgaaggca cgcgtggaca ggttcggcaa aaaagccagc ttcctgccca
     6601 agcatttttc cgacaaggtc atgcagttcg tcagcaaatt cctgcccgac cacctgccca
     6661 aatccatgcg cgattaccgc aataaatacg aacaccacct gattctgaaa atgggcggga
     6721 aaggcgtgga tgaggcgcgc gcattcttaa aagaatattt ttcacaccac ggcggcgcgt
     6781 ttttcgaatg caacgccgaa gaaacccaag ccgcgatgct gcaccgtttc gccgtcgcct
     6841 ccgccgccat ccgctaccgc gccgtgcatg atgacgaagt ggaagacttg gtcgcgctcg
     6901 acatcgccct gcgccgcgac gatcgcgact ggtttgaaaa actgccgtcg gaaatcgaca
     6961 ataaaatcat ccataaactc tactatggac atttcatgtg ccacgttttc catcaggatt
     7021 acatcatcaa aaaaggcaac gactgcatgg cgttggaaca cgaaatgctg catcttttag
     7081 accaacgcgg cgcgcaatat cccgccgaac acaacgtcgg tcatttgtat gaagccaaac
     7141 ccgcgctcaa acagttttac cgcaaacttg acccgaccaa cagcttcaac ccgggcgtag
     7201 gcaaaaccag caaaaagaaa aactgggcgg aataagcgcg tccgctttga aggcaggcaa
     7261 tgccgtctga aggcaaaacg ccgttcagac ggcattgtcg ggtttgaagt tccccgtcgg
     7321 gtaatcgctt tcaaaccgta tgccgacaca cgggcgcgcc cctcccaaat atagtggatt
     7381 aaatttaaat caggacaagg caacgaagcc gcagacagta cagatagtac ggcaaggcga
     7441 ggtaacgccg tactggttta aatttaatcc actataaagt ctgatcctac cttcccaaag
     7501 ggcagggttt caaccgaaaa ggaaacacga tgaaaccata caaaatctac acccatcccg
     7561 ccctgccgcc ccaagccgtc aaacaaggct ggtcgtggcc gggccttttg ttcggcacgc
     7621 tgtgggcgtg tttcaaaaga atgtggggtt tggggctggg gctgacgggc gcgatattcg
     7681 tgctggctgt gtttgcccaa ctggtttacg gcgacacgcc cgccacagac tcggcgttta
     7741 acgtattggg cttggcggtt tccgtctggt tcggcgcaaa gggcaacagc ctttatgcac
     7801 gccacctttt atcgcgcggc tataccgaat tgcccgaaac ggttgaggca gccaatcccc
     7861 aagccgcatt ggcgcaatat ttcgggcgcg ggggccgata agcgcgtttt cttttccatc
     7921 atgccgtctg aaaaatgttc agacggcatt tcttataacc ttttgtttgc agcacataac
     7981 caatcagtct ttcctattcc gttccgaaat gcataccatg cgcccgtccc caatatttgc
     8041 gaacaaggaa agaaaatggc acgtttaacc gtacacaccc ttgaaaccgc ccccgaagcc
     8101 gccaaagcgt gcgtagaggc ggtacttcaa aacaacggct tcatccccaa ccttatcggc
     8161 gtattggcaa acgcccccga agccttggcg ttttaccaag aagtcggcaa gctcaacgcc
     8221 gccaacagcc tgactgccgg cgaagtcgaa gtcatccaga tcatcgccgc ccgcaccaac
     8281 caatgcggct tctgcgtggc agggcatacc aaactggcaa ccctgaaaaa actcctttcc
     8341 gaacaatccg tcaaagccgc gcgcgctttg gcggcaggcg aatttgacga tgccaaactc
     8401 ggcgcgctcg ccgccttcac ccaagccgtg atggcgaaaa aaggcgcggt atccgacgag
     8461 gaactcaaag cattttttga tgcaggctac aatcagcagc aggcggtcga agtcgtgatg
     8521 ggcgtagcct tggcaaccct gtgcaactac gtcaacaacc tcgcccaaac cgaaatcaac
     8581 cccgaattgc aggcatacgc ctaagcaaac aaaaccgcct gaatgcaggc ggtttttcaa
     8641 aaacaccccc tcaaacaaaa acaagccgcc caagcggcag gggaagcagc cgcgcatcag
     8701 gcgcgccccc gattccctcg caaaccttaa ggaacaaaga tgaacgccca aaccctactt
     8761 accaacgttg ccgaactcgt caaaaccaag ctcaaaccca tagtggacga catcgaccgc
     8821 aaaggatact accccgaagc atttatgcgc gaactcggcg caatcggcgg attcggcgca
     8881 gtcggtacag aagccgaagg cggcaacggc ttgggtttgg caacgcaaat cgctgttttg
     8941 cgcgaaatcg gcaaagaatg cggcgcaacc tccttcagcg cgtggtgtca ggcggcttgc
     9001 gcgtggtatc tgcaccaaac gcccaaccaa gccgtcaaag acaaatacct cgccgacatc
     9061 ctgcaaggca aagtattggc aggcactgga atgtccaata ccgtcaaaca ccttgtcggc
     9121 attgaaaaac acaaccttca agccgagcgc gtggagggcg gctacacagt caacggcgcg
     9181 ttgccgtggg tgtccaacat cggcgaagac cacatctggg cgaataccgc ccaaatcggc
     9241 ggcggctacg ttatgttcat cacaggcggg caaagggaag gcgtaagcct gcaaaactgc
     9301 cccgaatttt gcgccctcga aggcacgcgc accttcagcc tgaacttcaa agacgtattt
     9361 atccccgacg aagacatcat cgccgcgccc gaacagtttg ccgactacat ccaaagcatc
     9421 aaagcaggct tcatcttgct gcaaatcggc atcggcgcgg gcgtgattga cggcagcctc
     9481 ggcatcatcc gcctcgccaa cgtcgtcaac gccgaagtca acagctatct cgacgacggc
     9541 tacgacagcc tcaaagcaag gttggacggc gcgtgggcag aaaccgaacg gctcgccgac
     9601 ttggcgtggg acggcgcgcc cgataacctc gccaccctca agctgcgcga agccgccgcc
     9661 gtactggcac ttgccgccgc ccaatccgcc gccctgcatt ccggcgcaaa aggctacctg
     9721 atgcgcagtc ccgcccaaag gcgcgtccgc gaagcaatgt ttgtcgccat cgtaaccccc
     9781 gcgatcaaac acctgcgcaa agaaatagcg gcaatcgaag ccgcaaaata aacaggcgga
     9841 aaaaacagaa acccccaccg cgtcattccc gcgaaagcgg gaatccagcc cccaacgcaa
     9901 caggaatcca tcggaaaaaa cagaaacccc caaaccgtca ttcccgcaaa agcgggaatc
     9961 cagcccccta acgcaacagg aatccatcga aaaaaacaga aaccctcaaa ccgtcattcc
    10021 cgcaaaagcg ggaatccagc ccccaacgta acaggaatcc atcggaaaaa acagaaaccc
    10081 tcaaaccgtc attcccgcaa aagcgggaat ccagccccct aacgcaacag gaatccatcg
    10141 aaaaaaccga aacctccaaa ccgtcattcc cacggaagtg ggaatccagt aaccgaaaaa
    10201 ccacaggaat ctatcgaaaa aaaacccaac ccccaaaaaa accgggcgga tacctcacca
    10261 tcctcccaaa ccctgattta tagcggatga acaaaaatca gggcaagacg gcgaaaccgc
    10321 agacagtaca aatagtacga aaccgattca cttggtgctt gagcacctta gagaatcgtt
    10381 ctctttgagc taaggcgagg caacgccgta ccggtttttg ttaacccact atacaaaaaa
    10441 caaggaaaca caaatggcgc aataatatgt gcggcccctg cggctggatt tacgatgaag
    10501 aacacggcga ccccgaacac ggcatcgccc ccgggacaaa gtttgaagac atccccgacg
    10561 actggaaatg ccccgaatgc ggcgtgggca aagaagattt ctacctgttg gatttcgtga
    10621 tatagcggca gccgcaccct tagggaaaac cccgtcatcc ggcgttccgc ctgcggcggc
    10681 agggaacacc gttttatagt ggattaacaa aaatcatgac aaggcgacga agccgcagac
    10741 agtacaaata gtacggaacc aattcacttg ttaaagaatc gttctctttg agctaaggcg
    10801 aggcaacgcc gtactggttt ttgttaatcc actatatccg tttgcgcccg ccaagccgtg
    10861 ttgcacagat gcgacacggc tttttccttt tcgtcgtccg aatgtaaatt ttcggcaaaa
    10921 aacg
//
LOCUS       DAWHRA010000068        10922 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWHRA010000068 DAWHRA010000000
VERSION     DAWHRA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.068"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(74..457)
                     /locus_tag="WIB30_001264"
     CDS             complement(74..457)
                     /locus_tag="WIB30_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ3797333.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGSILQFVCNWGFPAAEKTCSVLKKIGAVHSAMLIHRAADALDKEIRRLQS
                     EGKNLKEMWDITSRQQNRLTAEQSG"
     gene            complement(515..1891)
                     /gene="argH"
                     /locus_tag="WIB30_001265"
     CDS             complement(515..1891)
                     /gene="argH"
                     /locus_tag="WIB30_001265"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ3797334.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRCRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(1910..2779)
                     /gene="galU"
                     /locus_tag="WIB30_001266"
     CDS             complement(1910..2779)
                     /gene="galU"
                     /locus_tag="WIB30_001266"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ3797335.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(2807..3406)
                     /gene="rdgB"
                     /locus_tag="WIB30_001267"
     CDS             complement(2807..3406)
                     /gene="rdgB"
                     /locus_tag="WIB30_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ3797336.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDTEVKNRESHRAQALAELLRKLAL"
     gene            complement(3399..3632)
                     /locus_tag="WIB30_001268"
     CDS             complement(3399..3632)
                     /locus_tag="WIB30_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797337.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLHAAQSATKENRYV"
     gene            complement(3740..4273)
                     /locus_tag="WIB30_001269"
     CDS             complement(3740..4273)
                     /locus_tag="WIB30_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ3797338.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDVE
                     EAKKVIKESIERWNKQA"
     gene            complement(4375..4833)
                     /gene="nudB"
                     /locus_tag="WIB30_001270"
     CDS             complement(4375..4833)
                     /gene="nudB"
                     /locus_tag="WIB30_001270"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ3797339.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            5044..5120
                     /locus_tag="WIB30_001271"
     tRNA            5044..5120
                     /locus_tag="WIB30_001271"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5078..5080,aa:Pro,seq:ggg)
     gene            5279..6587
                     /gene="mafB"
                     /locus_tag="WIB30_001272"
                     /pseudo
     CDS             5279..6587
                     /gene="mafB"
                     /locus_tag="WIB30_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            6630..7172
                     /locus_tag="WIB30_001273"
     CDS             6630..7172
                     /locus_tag="WIB30_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ3797340.1"
                     /translation="MFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHTQ
                     REWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIK
                     KPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQKWRGTSKSG
                     VEIEGFTEPNRTAYPLYNSK"
     gene            7199..7618
                     /locus_tag="WIB30_001274"
     CDS             7199..7618
                     /locus_tag="WIB30_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797341.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            7696..7779
                     /locus_tag="WIB30_001275"
                     /pseudo
     CDS             7696..7779
                     /locus_tag="WIB30_001275"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7776..8018
                     /locus_tag="WIB30_001276"
     CDS             7776..8018
                     /locus_tag="WIB30_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ3797342.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            8096..8341
                     /locus_tag="WIB30_001277"
     CDS             8096..8341
                     /locus_tag="WIB30_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ3797343.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLVPEWEHSKTGHIGGTAMDKGK"
     gene            8344..8730
                     /locus_tag="WIB30_001278"
     CDS             8344..8730
                     /locus_tag="WIB30_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797344.1"
                     /translation="MWKIYKENSTDLNFAIGSIYCQAINLTEFKMWVEKIIREVDLDE
                     IPNYFFDLIDFQSLYDLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPVSKEKA
                     LKALKKHPEIYQRFQHFFPFVELPPL"
     gene            8832..9221
                     /locus_tag="WIB30_001279"
     CDS             8832..9221
                     /locus_tag="WIB30_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797345.1"
                     /translation="MWKIIKEDSDDLEFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIYVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            9248..9397
                     /locus_tag="WIB30_001280"
     CDS             9248..9397
                     /locus_tag="WIB30_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ3797346.1"
                     /translation="MRLSDGIFDIKRITKRAFDSKGTSEIPDYTWHHHQDTGCIGWKA
                     MNKRR"
     gene            9400..9786
                     /locus_tag="WIB30_001281"
     CDS             9400..9786
                     /locus_tag="WIB30_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797347.1"
                     /translation="MWKIYKENSTDLNFSLGSIYCQAINITEFKMWVEKIIREMDLDE
                     IPNYFFDLTDLQSLFHLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDSPISKEKA
                     LKALKKHPEIYQRFQHFFPFVELPPL"
     gene            9908..10849
                     /gene="mafA"
                     /locus_tag="WIB30_001282"
     CDS             9908..10849
                     /gene="mafA"
                     /locus_tag="WIB30_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ3797348.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATRYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGMGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
BASE COUNT         2782 a         2560 c         2836 g         2744 t
ORIGIN      
        1 cgtctgaaca gggttgctgg aatagtattg ccatcccagc agatacagtt tgtcggggtc
       61 ttgccaatat tgttcatcca gactgttcag cagtgaggcg gttttgttgt cgagatgtta
      121 tatcccacat ttctttcagg tttttacctt ccgattggag gcggcggatt tctttgtcca
      181 atgcgtctgc cgcccgatgg atcagcattg cgctgtgtac cgcgccgatt tttttcagaa
      241 cggaacaagt cttttcggcg gcggggaaac cccagttgca gacaaattgc agtatgctgc
      301 cgttaccgat atcggcttct gtccgccaaa tcaaaaccag ctcctgttcc cgcccgtcca
      361 tgctttcgag tttgccgtca tgctgttcaa aaagtttgtc caccgcctgc cacatcatct
      421 gttcgaagcg gtcggcttgg gtatccgtat cggtcatcgt gttcttgcct tttaaaaatg
      481 ccgtctgaac atttcttcag acggcatttg ggggttaagc caacatttcc cgccagcgtt
      541 tcacttggaa gcggacttgt tccggcgcgg taccgcccaa gtggttgcgg gcgtttaagc
      601 tgccttcggg tgtcagcacg ccgtaaacgt cgtcggcaat caaatcgctg aaaccttgta
      661 agacttcgag cggcagttcg ctcaaatcga cgcccgcttg gtcggcgtgg cgcacggctt
      721 gggcgacgac ttcgtgggca tcgcggaaag gcatgccttt tttgaccaga taatccgcca
      781 agtcggtggc ggtagcgaag ccctgcatca cggcggcgcg catattgtcg ggtttgacgg
      841 ttacgccgcg catcatatcg gcgtaaatcc gcaacgtgtc gataagcgtg tcggcggtgt
      901 cgaacaaggg ttctttgtct tcctgattgt ctttgttgta cgccaagggt tgggatttca
      961 tcagggtaat cagaccgata aggtgtccga tgacgcggcc ggatttgccg cgcacgagtt
     1021 cgggcacgtc ggggtttttc ttctgcggca tgatggacga acctgtgcag aaacggtcgg
     1081 cgatgtcgat aaagccgaaa cgcgggctca tccacaaaat caattcttca gacaggcggc
     1141 tcaggtgaac cataaccagc gaggcggcgg ctgtgaactc aatggcgaaa tcgcggtcgg
     1201 atacggcatc gagcgagttc tggcagattt gttcaaagcc caatagctcg gcggtgattt
     1261 cgcgctgaat cgggtaggtc gtcccggcaa gggcggctgc gccgagcggc atacggttga
     1321 cgcggcagcg gcagtccgcc atccgttcgt tatcgcgtcc gagcatttcg acgtaggcga
     1381 gcatatggtg tccgaagctg acgggctggg cgacttgcag gtgggtaaag cctggcatga
     1441 cggtttcggc gttttgttcc gccaaatcca gcaatgccgt ctgaaggctt tgaatcaggc
     1501 tttgtataac ggtaatctgg tcgcgcagcc acaggcggat gtcggtggcg acttggtcgt
     1561 tgcggctgcg gccggtgtgc aggcgtttgc ccgcgtcgcc gattttgtcg gtcaggcggc
     1621 gttcgatgtt catatggaca tcttccaaat cggacgacca ttcgattttg ccgctgcgga
     1681 tttcttcgag gatttccgcc ataccccggc ggatgtccgc caaatcgcct tcgtccaaca
     1741 cgccggtttc tttcagcatt tgcgcgtgtg ccagcgagcc ttggatgtcc cattcggcaa
     1801 gccgtcggtc gaaaccgatg gaggcggtgt attgtttgac gagttcggaa acgggttcgt
     1861 tgaaacgtcc ggaccaggtt ttgtcgtgca taaggattcc ttgatggggt tattcggtgc
     1921 ggtatttttc caaaagccgg cggaagggtt cgccggtttc gggatgtttc agaccgtagg
     1981 cgacggtggc ttcgaggtag cccagtttgc tgccgcagtc gtagcgcgta ccttcaaagg
     2041 ggtgcgccag gacaaattcg tgatcgagca gcttggcgat gccgtctgta agctggattt
     2101 cgttgcccgc gccgcgcgga agattggtta agaggtcgaa aatgcgcggg gtgaggatgt
     2161 agcgtccaac aacggcaagg ttggagggcg cgtcttcggg cttgggtttt tcgacaatgc
     2221 cggtaatgcg ttggaactgt ttgagctgtt cggtttcgac gatgccgtat gagccggttt
     2281 gcgatgcttc aacggtttct acgcccaaaa tgctgttgcc gctgcgcccg tacacttcga
     2341 ccatttgttt gagcgcgcct ttgggggcat caatcaggtc gtcggcaagg ataacggcaa
     2401 agggttcgtc tccgatggcg gcgcgggcgc acaagacggc gtgtcccaag cccagtgctt
     2461 ccgcctgacg gatgtagagg caggtaatgt tcggcggcag gatgttgcgg acgtgttcca
     2521 acaatttgtc tttatggcgc atttccaact cggtttcgag ttcgtatgcc ttgtcgaaat
     2581 ggtcttcgat gctgcgtttg ttgcgtccgg taacaaacac catttccgtg cagccggctt
     2641 ccacggcttc ttctacggcg tattggatca gcggcttgtc gacgatgggc agcatttctt
     2701 tcgggctggc cttggtggcg ggcaggaagc gggttcccat ccctgcgacg gggaaaacgg
     2761 ctttccgtat gggtttcatt ctttttcctt tgtattgttt tgatgtttaa agggcgagtt
     2821 tgcgtaagag ttcggcaagt gcctgcgcgc ggtggctttc gcggtttttg acctccgtat
     2881 ccaattcggc ggcggttttg ccgtgttcgg gcagataaaa atacgggtcg taaccgaaac
     2941 cgttttgccc gagcggcgtg tcgttccact gcccgtgcca tacgccctcg gcgataatcg
     3001 ggcgcgggtc gtctttatgg cggacaaaaa ccaatacgca gacatagcag cagcttttgt
     3061 ctgccttgcc gacaagttcg gcggcaagtt tcaggttgtt ggcggtatcg gatttgggat
     3121 tgtcgcccgc gtaacgtgcg gaatggatgc ccggcgcgcc gtttaaggcg gcggcacaga
     3181 tgccgctgtc gtcggcgagt gcgggcagcc cgctgtattt ggcggcatgg cgtgcttttg
     3241 ccagcgcgtt ttcgacaaag gtgggatagg gttcggggca ttcgggtatg ccgaatgcgg
     3301 attgcggcaa tacggtgatg ctgtaaggtt tgaataagtt gccgaactct tcgagcttgc
     3361 cggcattgcc gcttgccaaa acgatttttt ccggtttttc agacatagcg gttttccttt
     3421 gtggcggatt gggcggcgtg tagggatttg tgccgcaaat agagggcgag tgcgccgatt
     3481 tgcccgaaca gcgcgccgaa tgcgaaaaga aaggaggcga cggcgaaggc tttgctgccg
     3541 acggtcagta gataagcacc caagaggacg agcagcataa atgccagtgt gaacagggca
     3601 acagacaaca gatagatttt tcggcggttc atggcgttcg gtcggaaacg gtatgttcgg
     3661 attatatccg attgggacgg cattccctag agcttggaaa aatgccgtct gaaggggcgt
     3721 tcagacggca tggcgggcgt tatgcctgtt tgttccagcg ttcgatggat tctttgatga
     3781 cttttttcgc ttcttccaca tcgccccagg attcgacttt ggtcgtacct gcttttttca
     3841 ggtctttgta atggttgaag tggaactcga tttgtttgat gagctgttgc ggcaaatcgg
     3901 acaaagtttt gtaggcgttg ccgttgttgc ggtcgtcggc aggaacgcag acgattttgt
     3961 cgtccacttc gccgtcgtca acgaatttca tcacgccgat aacgcgcgct tccaagaata
     4021 cgccggttgc cagaggctgt tcggtaacga gcagcacgtc caattcgtcg ccgtcttcgt
     4081 ccaaagtttg gggaatgaag ccgtagttgg tcggtttggc gaagatggcg ggttcgacgc
     4141 ggtcgagttg gaatgcggcg agtttgcggt tccactcgat tttgtggttg ctgccggcgg
     4201 ggatttcgtt gacgacgttg atgatgccgc cgtccacatc gccgggggtc aggatttggt
     4261 tgaagtctgc catttggttt cctttgtttg ggaaagtttg aagtttgaaa gtatagcaca
     4321 aacgtccggc tgaaaatgcg cccgatgccg tctgaaaggg tgtacgggcg cgtgttaccg
     4381 tttgcccaaa aacctgccca gttccaaaat tgcgcgcctg ttggacgggg aaaacacttt
     4441 ttccgccgct tcttccaagc cgaaccagcc gtaggagacg tgttcctcgg gttgcaggac
     4501 gacgggcgta tcgcgcggga tttcggcaga gaagaggtgt tcgcggtttt caaacacgcc
     4561 ttttggatag cggtgccgcc agtggtggta gatttcgtaa accgtgctgt cgtgccagtc
     4621 ttgaagctgc ccgtccgcca gcaggatgcc ggtttcttcc caaacttcgc gccttgccgt
     4681 ttgggcgacg gtttcgcctg gttcgaggct gccggttacc gactgccaaa atcctttcgg
     4741 atgcgtgcgt tcgatgagca ggatgccgcc gtccccgcta taaaggacga ccagtgcgga
     4801 aacggggtat ttgagcggtt ttgccatcgg catctttcgg cgggctgcgg taatgaaggg
     4861 gcggattata gcaaacgccg cacgttatgg cgtttatcct tttccgtatc cttttttctg
     4921 cacggatggg acgcgccggt gtttgccggt aaattttccg ttgtgtcaaa aagataaggg
     4981 cggttgtgat tttaatgctt gccaaagcgt cgggcggaaa ctataatccg aaacttatcg
     5041 agtcggagtg tggcgcagtc tggtagcgca cttgcatggg gtgcaagggg tcgaaggttc
     5101 gaatcctttc actccgacca aaaattccga aagccgcttt caaaagcggc ttttttgccg
     5161 tccgtatgat tatgatgtag agtacgcggc gacagacatt caaatgccgt ctgacaaacc
     5221 gttcagatgg catctcttta tcttagtttc attccgtacc atcttaagga acatcaaatt
     5281 gggcatttcc cgcaaaatat cccttattct gtccatactg gcagtgtgcc tgccgatgca
     5341 tgcacacgcc tcagatttgg caaacgattc ttttatccgg caggttctcg accgtcagca
     5401 tttcgaaccc gacgggaaat accacctatt cggcagcagg gggggaactt gccgagcgca
     5461 gcggtcatat cggattggga aacatacaaa gccatcagtt gggcaacctg atgattcaac
     5521 aggcggccat taaaggaaat atcggctaca ttgtccgctt ttccgatcac gggcacgaag
     5581 tccattcccc cttcgacaac catgcctcac attccgattc tgatgaagcc ggtagtcccg
     5641 ttgacggatt cagcctttac cgcatccatt gggacggata cgaacaccat cccgccgacg
     5701 gctacgacgg gccacagggc ggcggctatc ccgctcccaa aggcgcgagg gatatataca
     5761 gctacgacat aaaaggcgtt gcccaaaata tccgcctcaa cctgaccgac aaccgcagca
     5821 ccggacaacg gcttgccgac cgtttccaca atgccggcgc tatgctgacg caaggagtag
     5881 gcgacggatt caaacgcgcc acccgataca gccccgagct ggacagatcg ggcaatgccg
     5941 ccgaagcttt caacggcact gcagatatcg tcaaaaacat catcggcgcg gcaggagaaa
     6001 ttgtcggcgc aggcgatgcc gtgcagggta taagcgaagg ctcaaacatt gctgtcatgc
     6061 acggcttggg tctgctttcc accgaaaaca agatggcgcg catcaacgat ttggcagata
     6121 tggcgcaact caaagactat gccgcagcag ccatccgcga ttgggcagtc caaaacccca
     6181 atgccgcaca aggcatagaa gccgtcagca atatctttat ggcagccatc cccatcaaag
     6241 ggattggagc tgttcgggga aaatacggct tgggcggcat cacggcacat cctatcaagc
     6301 ggtcgcagat gggcgcgatc gcattgccga aagggaaatc cgccgtcagc gacaattttg
     6361 ccgatgcggc atacgccaaa tacccgtccc cttaccattc ccgaaatatc cgttcaaact
     6421 tggagcagcg ttacggcaaa gaaaacatca cctcctcaac cgtgccgccg tcaaacggca
     6481 aaaatgtcaa actggcagac caacgccacc cgaagacagg cgtaccgttt gacggtaaag
     6541 ggtttccgaa ttttgagaag cacgtgaaat atgatacgaa gctcgatatt caagaattat
     6601 cggggggcgg tatacctaag gctaagcctg tgtttgatgc gaaaccgaga tgggaggttg
     6661 ataggaagct taataaattg acaactcgtg agcaggtgga gaaaaatgtt caggaaacga
     6721 gaagaaggag tcagagtagt cagtttaaag cccatacgca acgagaatgg gaaaataaaa
     6781 cagggttaga ttttaatcat tttataggtg gtgatatcaa taagaaaggc acagtaacag
     6841 gagggcatag tctaacccgt ggtgatgtac gggtgataca acaaacctcg gcacctgata
     6901 aacatggggt ttatcaagcg acagtggaaa ttaaaaagcc tgatggaagt tgggaaatga
     6961 aaaaaggaag gggcggaaaa cctatgacaa atcattctat gttcccaaaa gattgggatg
     7021 aggctagaat tagggctgaa gttacttcgg cttgggagaa aagaaaggaa ttagaaggtc
     7081 aaaaatggag aggtacaagt aaatctggtg ttgaaataga aggatttacg gaacctaatc
     7141 gaacagctta tccactttat aatagtaaat gaagtattga ttaaaaaggt gtttttaaat
     7201 gtataaaata aaatgttttt atgatcagtt tagttctggt gaattattta actactgcca
     7261 gtttttagat aattcttcta atcaaaagat aaatacacga ggaacggtgt atcaatatat
     7321 tatttatgtt ttaacaggag acctttatct acaaaaagac atagatgaaa atttagaatt
     7381 tatccatcaa gcagaaaata atcccaatga agtttattca ggaggtggac aaggtttttg
     7441 ctgggatatt tctgctgaaa aagtagtttt ctatcacaac gaatttgatg aagaagacgg
     7501 ctggcctgat ttaagctgtt cgctgcatac ttttaaaact gctttaattg cttggaatgc
     7561 ttttttgcaa ttgcctaaaa gtattcattc ggtggtggag actgtgattg aggaataagc
     7621 ataattagct taatgaatag aatcagcgat atagattgga ctgcaaatcc acgcttatac
     7681 gctgtgccat gattaacagg aagagagcaa cttggatggg ctagtaggga tggtaagcat
     7741 ttaaatatat caattgatgg aaagattaca cacaaatgac taatttaaaa ttagatttct
     7801 actctgaagt tattataaaa gattcttgcc ctaatgattt gttagaaaat ggtgagacta
     7861 ttaaaggaaa aaaaggagtg gtattaggta taagcgaaga agatggtata atctatggct
     7921 atactatttt actttttgat ataaaatatt gtatatatat agataagaaa tatattatac
     7981 cgactggaaa aaaattctct cgcgatgatt tttattagct aatttaagta atttaagcaa
     8041 gttttttaga tactgaaact ggtaattatg tttatgtaag cataaggagt tcagattgaa
     8101 ggattctaca gggcaaatga ggctggcaac caaggatttg gcggaagcca ttaaacgagg
     8161 agaagtacgt agttctgctt ttacaacaaa gcaactaaag gcaatcgaaa aaggagaatc
     8221 taaaataccg gattatactt ggcatcatca tcaggatact ggacgtatgc agcttgtacc
     8281 tgaatgggaa cattctaaaa ccggtcacat aggagggacg gcaatggata aaggtaaatg
     8341 attatgtgga aaatttataa agaaaatagc accgacttaa attttgccat aggcagtata
     8401 tattgccaag caatcaatct taccgaattt aaaatgtggg tcgaaaaaat cataagggaa
     8461 gtggatttag atgaaattcc aaattatttt tttgatttga tagattttca atcactatac
     8521 gatctgattg atattatagg atttgttccc gaaaataatt tatcaaaaaa tcaggataat
     8581 gcattaacag gtattgcttt cttaagaggg atagatgtct atgaccctcc cgtttcaaaa
     8641 gaaaaagcat taaaagcctt aaagaaacat cctgaaattt atcagaggtt tcagcatttc
     8701 ttcccatttg tagaactgcc cccgctttaa acagtcaaaa tgccgtctga aacgatattc
     8761 ggcgttcaga cggcattttt tgatacaaag caggtaacca aaagagtgtt tgacggcaaa
     8821 ggaagataat tatgtggaaa atcataaaag aagatagtga tgatttagaa tttgcaatta
     8881 aatgcttatt ctctcaatct attgatttaa atgaattcaa gttatggatt gaacaagtaa
     8941 tacgagatat gcccatcgag gacatccctt tttatatttt tgacttggcg gattttgatg
     9001 ggggaattgc cgatattgac aatattgtag gttttgtttc gagttacagc ctatcaaaat
     9061 cgaaaaaaaa tgccttgaca ggcattgcct tcttaagggg gatatatgtc tatgatccgc
     9121 ctatttcaaa agaaaaagca ttaaaagcct taaagaaaca tcctgaaatt tatcagaagt
     9181 ttcagcattt ctttccgttt gtagaactgc ccccgcttta aacagtcaaa atgccgtctg
     9241 aaatgatatt cggctttcag acggcatttt tgatataaag cggataacta aaagagcgtt
     9301 tgacagcaaa ggaacatctg aaataccgga ttacacttgg catcatcatc aagatacagg
     9361 atgtattggt tggaaagcga tgaacaaaag aagataatta tgtggaaaat ttataaagaa
     9421 aatagcactg acttaaattt ttccctaggt agtatatatt gccaagcaat caatattacc
     9481 gaatttaaaa tgtgggtcga aaaaatcata agggaaatgg acttagatga aattccaaat
     9541 tatttttttg atctaacaga tcttcaatca ctattccatc taattgatat tataggattt
     9601 gttcccgaaa ataacttatc aaaaaatcag gataatgcat taacaggcat tgccttctta
     9661 agggggatag atgtctatga ttcgcctatc tcaaaagaaa aagcattaaa agccttaaag
     9721 aaacatcctg aaatttatca gaggtttcag catttctttc cgtttgtaga actgcccccg
     9781 ctttaaacag tcaaaatacc gtctgaaagc catttccgcc gctcagacgg cattttttcc
     9841 tctttcgttt acaaaccctt aaaatccatt tacactcaaa ctcgttcaaa acaaaacaaa
     9901 ccctgccatg aaaaccctgc tcctcctcat ccccctcgtc ctaaccgcct gcggcacact
     9961 gaccggcata cccgcccacg gcggcggcaa acgctttgcc gtcgaacaag aactcgtcgc
    10021 cgcatcgtcc cgcgccgccg tcaaagaaat ggatttgtcc gccctgaaag gacgcaaagc
    10081 cgccctttac gtctccgtta tgggcgacca aggttcgggc aacataagcg gcggacgcta
    10141 ctctatcgac gcactgatac gcggtggcta ccacaacaac cccgaaagtg ccacccgata
    10201 cagctacccc gcctacgaca ctaccgccac caccaaatcc gacgcgctct ccagcgtaac
    10261 cacttccaca tcgctcttaa acgcccccgc cgccgccctg actagaaaca gcggacgcaa
    10321 aggcgaacgc tccgccggac tgtccgtcaa cggcatgggc gactaccgca acgaaaccct
    10381 gctcgccaac ccccgcgacg tttccttcct gaccaacctc atccaaaccg tcttctacct
    10441 gcgcggcatc gaagtcgtac cgcccgaata cgccgacacc gacgtattcg taaccgtcga
    10501 cgtattcggc accgtccgca gccgtaccga actgcacctc tacaacgccg aaacccttaa
    10561 agcccaaacc aagctcgaat atttcgccgt cgaccgcgac agccggaaac tgctgattgc
    10621 ccctaaaacc gccgcctacg aatcccaata ccaagaacaa tacgccctct ggatgggacc
    10681 ttacagcgtc ggcaaaaccg tcaaagcctc agaccgcctg atggtcgatt tctccgacat
    10741 taccccctac ggcgacacaa ccgcccaaaa ccgtcccgac ttcaaacaaa acaacggtaa
    10801 aaaccccgat gtcggcaacg aagtcatccg ccgccgcaaa ggaggataaa ccgtgaaacc
    10861 gctgcgcaga ctgacaaacc tccttgccgc ctgcgccgta gcggccgccg ccttcggaca
    10921 gc
//
LOCUS       DAWHRA010000069        10804 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWHRA010000069 DAWHRA010000000
VERSION     DAWHRA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10804
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.069"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(107..1324)
                     /locus_tag="WIB30_001283"
     CDS             complement(107..1324)
                     /locus_tag="WIB30_001283"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ3797349.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1524..2252)
                     /gene="rph"
                     /locus_tag="WIB30_001284"
     CDS             complement(1524..2252)
                     /gene="rph"
                     /locus_tag="WIB30_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ3797350.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2334..2798)
                     /locus_tag="WIB30_001285"
     CDS             complement(2334..2798)
                     /locus_tag="WIB30_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ3797351.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTAQELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3015..3791
                     /locus_tag="WIB30_001286"
     CDS             3015..3791
                     /locus_tag="WIB30_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ3797352.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            3929..4456
                     /locus_tag="WIB30_001287"
     CDS             3929..4456
                     /locus_tag="WIB30_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797353.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKICILRTSFI
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRSS
                     TDYIVSRNLKFRHLY"
     gene            4810..5664
                     /locus_tag="WIB30_001288"
     CDS             4810..5664
                     /locus_tag="WIB30_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ3797354.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPKQGAAYVVVNFIALLELAKEGLVRIVQEDGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5705..6913
                     /gene="pncB"
                     /locus_tag="WIB30_001289"
     CDS             5705..6913
                     /gene="pncB"
                     /locus_tag="WIB30_001289"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ3797355.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEAELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            7005..8723
                     /gene="argS"
                     /locus_tag="WIB30_001290"
     CDS             7005..8723
                     /gene="argS"
                     /locus_tag="WIB30_001290"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ3797356.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGIAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVVRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERAAALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSNYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLSEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAESASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8818..9193
                     /locus_tag="WIB30_001291"
                     /pseudo
     CDS             8818..9193
                     /locus_tag="WIB30_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9289..10707
                     /locus_tag="WIB30_001292"
     CDS             9289..10707
                     /locus_tag="WIB30_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ3797357.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2589 a         3055 c         2671 g         2489 t
ORIGIN      
        1 attttttgtt ccaaagtcaa aatatgccgt ccgaacattc gggcggcaga caaaacggca
       61 ctgcccgata aaggcagtgc cgttgtccgt ttcaaaccgt gaaacatcag cccaaattaa
      121 aggctttatg caataccctg gttgccagtt ccatgtattt ttcatcaatc aatacggaaa
      181 ctttgatttc ggaggtggaa atcatttgga tgttgatacc ctcttcggcg agcgtgcgga
      241 agattttggc ggctacaccg acgtgcgaac gcatacccaa accgactgcg gagactttgc
      301 atacggtgtc gtcgccatca atagaagccg cgccgatact gtcttggcgt tccgacagga
      361 tttccaaagt ctgcttgtaa tcgccgcgcg gtacggtaaa ggaaaaatcg gttgtgcctt
      421 cgctgccgac attttggata atcatatcga cttcgatgtt ggcatcggca accgcgccta
      481 aaatctgata ggcgacgcca ggtttgtcgg gtacgccgcg cacgttgatg cgggcttggt
      541 ttttatcgaa tgcgataccg gttacggcag ctctttccat gttgtcgtcc tcttcaaagg
      601 taattaaggt gccattgcca ccgtcttgca ggctgctcag tacgcgcagg cgcactttgt
      661 attttccggc gaattctact gaacggattt gcaaaacttt cgaaccgagg cttgccagtt
      721 cgatcatttc ttcaaatgta accgtatcca tgcggcgcgc ttcgggtacg acgcgggggt
      781 cggttgtgta aacgccgtct acgtcggtat agatttggca ctcgtcggct ttgagcgcgg
      841 cggcaagcgc gacggcggaa gtgtcggaac cgccgcgtcc gagcgtggaa atatcgcctt
      901 cactgctgat gccttggaag ccggcaacga tgacgacttt gccggcggta aggtcggcac
      961 gcattttttc gtcatcaatg ctttcgatgc gggctttggt gtgggcggta tcggttttga
     1021 gggcgacctg ccagcctgtg tagcttttgg catccacgcc gatgtctttc aatgccatcg
     1081 ccaaaaggcc gatggttact tgttcgccgg tagctaagac gacgtccagc tcgcgcggat
     1141 cgggatgctc ttgcatttcg tgcgccagtg cgaccagtcg gttggtttcg ccgctcatgg
     1201 cggatacgac gactacgatg tcgtgtcctt cggcgcgggc tttggcgaca cgtttggcta
     1261 cgtttttgat gcgttcgggc gagcctactg atgtgccgcc gtatttatgt acgattaacg
     1321 ccatgtttcg tgctttcttg tgggggttgt cgggcagctt ggtttgctgg aaaaagggtt
     1381 attattacta ttttttacat ggaattcaag aacggactgc gctttcccgc ctgccgtttg
     1441 acagcggtca gcgaaaaacc tgttctttca gattgttgac aaaatgccgt ctgaacggtt
     1501 ttcagacggc atccggacga caatcaggcg gcggacaacg cattttgctg gtgttgcagc
     1561 agttcgccta tgcctttttg cgccagtgca accagtttgc ccaattcgtc caaactgaac
     1621 ggcgcgtctt ccgccgtccc ctgtatttcg atgatttttc ccgatgcggt catgacgata
     1681 ttcacatcac tgtcgcaacc ggagtcttcg ggataatcca aatccaaaag cggcacgccg
     1741 ttcactacgc ctactgacac agcggcaacg gcttcgcgga tggggttttc actcaaaatg
     1801 ccgtctgaaa ccagtttgcc gacggcgatt tgcagcgcga caaacgcacc ggtaatcgaa
     1861 gccgtgcgcg taccgccgtc tgcctgaatc acatcgcagt caatcaagat ttgtcgttca
     1921 ccgagttttt ccatatccac gaccgcgcgc agggaacgcc cgatcaaacg ttggatttct
     1981 tgtgtgcgcc cggactgttt gcccgccgaa gcttcgcggc gcatccggga agcagttgag
     2041 gcaggcagca tcccgtattc cgccgttacc cagccttggt ttttaccgcg cagaaacggc
     2101 gggacgtttt catctatgga agcggtacaa atcactttgg tattgccgca ttcaataagg
     2161 cacgaaccgt ccgtatgcgg caggaaatga ggggtgattt tgatatcgcg caggctgtcg
     2221 gcggcgcgcg agatgcggat gtaatcaggc atactgccct cccgttaaaa acagataaat
     2281 taaaaagcct taaatatgaa aaatcacatt taaggccttc aaactgaaaa tttctacgcc
     2341 tcttcggctt tgctgcggat aatcaaaagc ggcaggtggc tttggcgcat taccgtttcg
     2401 gcaaaactgc ccattaaaag gtgcatcagc ccggtacgtc cgtgcgtacc caacaccagc
     2461 aggtcggcac cgttttcatc ggcataatca accaaatcct gcgccatttc acgcgcaccc
     2521 ttattggcaa ccagcaggtg tttgacggta ttttccacac ccagttcctg ggcggtgcgc
     2581 tcggcggcat ccaaaacttc gttgccttgc gcgacggcgg cggcttcgta gctttcgtgt
     2641 tgcaaaaatt cgggggcgag tgccatatat tcggcaggat tggcaacgtg caccaaagtc
     2701 aggcgcgcac cgttgacccc ggcaagctcg gcggcatgtt tcagggcatt gatggacgtt
     2761 tcactgccgt caacggcaac aaccaaatgt ttgtacatat cgtattctcc ttttgcaccg
     2821 cctcgcggtg ccctcttgtc ggatgggcgc agggacagtt tgcgctgttt cattatagac
     2881 ccgccgtcgg gctttataca acagccgaac agcccgaccg ctttccagta taatatgccg
     2941 cttccgtgca gtcaggcatt ttttgccggc tttcgttcac tttttgattt gacgcaatct
     3001 tgcaggattc gaccatgtcc gacaacgctt tgacctcttc gcgacgcttc ggcggcatcg
     3061 ccagactcta cggagactct gccttggcgc acttttcaca ggcacacgtc tgcgtagtcg
     3121 gcgtgggcgg tgtcggctcg tgggcggtcg aggctttggc gcggacgggc atcggacgtt
     3181 tgactttgat tgatttggac aacgttgccg aatcgaatgt caaccgccag ctgcacgccc
     3241 tgaccggcga cttcggcaaa gcaaaagtta ccgccttgcg cgaacgcatt acacaaatta
     3301 atccgcaatg cgaagtgttt gaaattgaag atttcgttac cgaagacaat ttgccggaat
     3361 acttcggaaa aggttttgat ttcgtcatcg acgcgatcga ccaagtgcgc gtcaaagcag
     3421 caatggcggc ttattttgtg gaacgcaaac aaccgtttgt cctcagcggc ggcgcgggcg
     3481 gacaaaaaaa tccggcgtta atccaaaccg ccgatttgag ccgcgtaacc cacgacccgc
     3541 tgcttgccaa cctgcgctac accttgcgga aacgctacgg attcagccgc gatacgaaag
     3601 caaatatgcg cgtgccttgc gtgtattcga ccgaaaatat cgtgccgccg cagtctaggg
     3661 aggcttgttc ggcagatgcc gctccgcaag gcttgtcgtg cgccggctac ggtgcaagca
     3721 tgctcgttac cgcttcgttc gggctatatt gcgcacaggc ggcggtggaa cacatcgcag
     3781 acaaaaaata agcaatgccg tctgaaacag gattcagacg gcatttgaac aaactatggt
     3841 tatgatttaa gacaacaaag gatacggata aaaaataaca taaaatatat gattcctaat
     3901 aatataccaa gtatcggaga gctatttaat ggaattcgtt aataatttag ttattttttc
     3961 atttttatta ctaatgctta ttccgatatt ttttgtagta tatggtatat accataagat
     4021 acgttatcgc aaaatatgta tcctaagaac aagttttata ttattagtgg taatactttg
     4081 cagtatgtat tacatatatt gccgttatct tgaccaacaa aaagtagctt attattgcat
     4141 agatgaacaa tgtatttcta ttgttcatct atacaaagat tatggtataa actctcccac
     4201 atatgcgaga atttacgcag gaaaaatatt gtttagattt caagtaagag ctaaaaatta
     4261 cgctgaatta cttatggaag atgatatatc aattagtaaa aaaattttgg ggaataaatt
     4321 tatcatttat gggtcgctac ctgtaatata cggtaatgta gataatattg aagtaaaaga
     4381 agctactggt tatatagata gatccagtac tgattatatt gtctcaagaa acttaaaatt
     4441 cagacattta tattaattaa gaggttttag caagagtgcc gtcaaaatat agggcgcatc
     4501 atcgaattcg cgaaagacaa acgctacgat gaacgtttca aggatttgaa aaaagaatcc
     4561 ataggctatc tgaaccggca tcccggtttg gtgtccgact acctgaaggc ggcaatcaag
     4621 ctgtcggttc agaaaaacca acatcagcac gcctaaaacc gtattcacaa cctgctcctt
     4681 ttcaaaacat ttgcatttaa aagccgttat aatgccgtct gaacatctgc ccgaccacat
     4741 tatacgtgaa tgtcggcaga ttgttttctt ttgtaaactt atattaaaat ccacttaccg
     4801 attcacgcca tgccgcccat ccctgcccca tctgcaccat ccgagcacac tgtcgcatgg
     4861 gtattcggcc aacccgttac cgatttgccc caggatttgt ttattccgcc cgatgcattg
     4921 aaagtcgtat tgggcagctt ccaaggccct ttggatctac tgctgtatct gatccgcaaa
     4981 cagaatatcg acgtactgga tattccgatg gtgaagatta ccgagcagta tctgcactac
     5041 atcgcccaaa tagaaaccta tcagtttgat ttggcggcgg aatatctttt gatggcagca
     5101 atgctgattg aaatcaaatc gcgcctgctg ctgccgcgta ccgaaaccgt cgaagacgaa
     5161 gaagccgacc cgcgtgccga gttggtgcgc cgcctgctgg cttacgaaca gatgaagctg
     5221 gcggcgcagg gtttggacgc gctgccccga gccggacggg atttcgcgtg ggcttacctg
     5281 ccgctggaaa ttgccgtcga agccaagctg cccgaagtct atattaccga cttgacgcaa
     5341 gcgtggctgg gtattttgtc tcgggcaaaa cacacgcgca gccacgaagt aatcaaagaa
     5401 accatctccg tgcgcgcgca aatgacggca atcctgcgcc gtttgaacgg acacggaata
     5461 tgcaggtttc acgacctgtt caatcccaaa cagggcgcgg cttacgtggt cgtcaacttc
     5521 atcgcactgt tggagcttgc caaagaagga ttggtcagaa tcgtgcagga agacggtttc
     5581 ggagaaatcc gaatcagcct caatcatgag ggggcgcatt cagacggcat ttccggcaca
     5641 cgaggcgggc gcgatgtgtt ctaatacgcc ccaagccgcc accaaaaatc gggagacacg
     5701 ccatatgacc ggcatcatac attcgctgct tgacaccgac ctctacaaat tcactatgct
     5761 gcaagtggtt ctgcaccagt ttccgcagac gcacagcctt tacgaattcc gctgccgcaa
     5821 cgcctcgacc gtctatccgc ttgccgacat cagggaagac ttggaagccg aactcgacgc
     5881 gctctgccaa ctacgcttca cccacgacga actcgactac ctccgtagcc tgcgtttcat
     5941 taaaagcgac tttgtcgatt atctcgaact cttccagctc caacgccgct ttgtcgaaat
     6001 cggcacagac gataaagacc gtctgaacat ccgcatcgaa ggtccgatga tacaggcgat
     6061 gttttttgaa atcttcatcc tcgccattgt caacgaactt tacttccgcc gcctggaaac
     6121 ccctgcagtc atagaagaag gcgaacgccg gcttcaagcc aaagccgcgc gcctcaaaga
     6181 aatcgccgcc gcacaaaacc ccgacgaacc gcccttcctg atttccgact tcggcacgcg
     6241 ccgccgctac aagctcgcgt ggcaggaaca cgtcatccgc accctgctcg aagccgcccc
     6301 aagcatcgtg cgcggcacca gcaatgtctt tctcgccaaa aaactcggca tcacccccat
     6361 cggcaccatg gcgcacgagt tcctccaagc cttccaggcc ctcgacgtac gcctgcggaa
     6421 tttccaaaag gccgcgctcg aaagctgggt gcacgaatac cggggcgatt tgggcgttgc
     6481 cctgaccgac gtggtcggta tggatgcctt cctgcgcgat ttcgacctct atttcgccaa
     6541 acttttcgac gggctgcgcc acgacagcgg cgacccttac gtttggggcg acaaagccta
     6601 cgcccactat caaaagctca aaatcgacag ccgcaccaaa atgctgacct tctccgacgg
     6661 gctggacatc gaacgctctt gggcattaca ccaatatttc aaagaccgct tcaaaaccgg
     6721 cttcggcatc ggcaccaacc tgaccaacga tatggggcat acgcctttga atatcgtctt
     6781 gaaactggtc gaatgcaacg ggcagtccgt cgccaagctg tccgactctc cgggcaaaac
     6841 catgaccaac aacagcacct tcctcgccta cctgcgccaa gtgttcgacg tacccgaacc
     6901 cgaaacgccg taaaccggca gaaaaagcgc acaattcctg tttctgccgc ataaaatctt
     6961 ttaaaatacc gcctgatttg aatttaaccg aaagaccgaa cttcatgaac ctacatcaaa
     7021 ccgtcgaaca cgaagccgcc gccgcctttg ccgccgcagg catcgccggc agccccgttg
     7081 ttttgcagcc gaccaaaaat gccgaacacg gcgatttcca aatcaacggc gtgatgggtg
     7141 cggcgaaaaa agccaaacaa aacccgcgcg aactggcgca aaaggtcgcc gacgcattgg
     7201 caggcaacgc cgtcatcgaa agcgcggaag tcgccggtcc cggttttatc aacctgcgcc
     7261 tgcgccccga atttctcgcg caaaacattc agacggcctt gaacgacgcg cgtttcggca
     7321 tagcgaaaac cgacaaaccg caaaccgtcg tcatcgacta ttcctcgccc aatctggcga
     7381 aggaaatgca tgtcggtcac ctgcgttcca gcatcatcgg cgacagcatt tcgcgcgtgt
     7441 tggcatttat gggcaacacc gtcgtccgtc aaaaccacgt cggcgactgg ggtacgcagt
     7501 tcggcatgtt ggtcgcttat ttggtcgagc agcaaaaaga caatgccgcg tttgagctgg
     7561 cggatttgga gcagttttac cgcgccgcca aagtgcgctt tgacgaagat cctgccttcg
     7621 ccgacaccgc gcgcgaatac gttgtgaagc tgcaaggcgg cgatgaaacc gttttggcat
     7681 tgtggaaaca gtttgtcgat atttcgctct cgcacgccca agccgtttac gacacgctgg
     7741 gcttgaagct gcgccccgaa gacgtggcgg gcgaatcgaa gtacaacgac gatttgcagc
     7801 cagtggtcga tgatttggtt caaaaaggtc tggcggtcga agacgacggc gcgaaagtcg
     7861 tattcttgga cgagttcaaa aacaaagaag gcgaacccgc cgcgtttatc gtgcaaaaac
     7921 aaggcggcgg cttcctttat gcttccaccg acttggcgtg tttgcgctac cgtgtcggca
     7981 cgcttcatgc cgaccgcctg ctgtacgtcg tcgaccaccg ccaagccctg cactttgagc
     8041 aactctttac cacttcccgc aaagcaggct atctgccgga aaacgtcggc gcggcatttg
     8101 tcggcttcgg cacgatgatg ggcaaagacg gcaaaccgtt caaaacacgc agcggcgaca
     8161 ctgtaaaact ggtcgatctg ctgaccgaag ccgtcgaacg cgccgccgct ttggtgaaag
     8221 agaaaaatcc cgaattgggc gcggacgaag ccgccaaaat cggcaaaacc gtcggcatcg
     8281 gcgcggtcaa atacgccgac ttgagcaaaa accgcaccag caattatgtg ttcgactggg
     8341 acgctatgct ttcgtttgaa ggcaacaccg ccccctacct gcaatatgcc tacacccgcg
     8401 tgcaaagcgt gttccgcaaa gccggtgaat gggacgcaac cgcaccgact gttttgagcg
     8461 aaccgctgga aaaacagctt gccgccgagc tgctgaaatt cgaagacgtg ctgcaaagcg
     8521 tggcggacac ggcgtatccg cactacctcg ccgcctacct ctatcaaatt gcgaccctgt
     8581 tcagccgctt ctacgaagcc tgtccgatac tcaaagccga aagcgcaagc cgcaacagcc
     8641 gcctgcaact ggcgaagctg accggcgaca cgctgaaaca aggcttggat ttgctgggca
     8701 tcgatgtgtt ggacgtaatg taaaaccgca ccgcccgatt gcggacaaca gcctcgccat
     8761 ccttatccga atctgaaaaa agcggcgcga tacaccgtat ccgccgcccc tcccaaaatg
     8821 cgaaacaaac aaacgccaag caagcaagca agcaagcaag caagcaagca agcaagcaag
     8881 caagcaagca agcaagcaaa aaattataac ccccttcctg ccgacgcacg cactttccgc
     8941 gcggcgcatt ccccttttcc cgcccctcaa atccgccttt tcttcaggca gggtttcagc
     9001 ccgcctcttt tccctgtttt cctttccccg acacgcgtgc gcgccccctg ccgcactgtg
     9061 ctgcactttc gcgcccggac ggcatcgttc cgccatccgg ttctctgttt taaatacccc
     9121 tgtttcagaa agaaatgcag atgtttcaac acacaggacg acacataaag caccgcccta
     9181 tgtgttgccc tgatttggaa ggggttacgc ctcccaaata aagtctgatc ctgccgcccc
     9241 gaaggacaga tgtccgagtg gcgaagtttc aaccgaaaag gaaatacgat gaatattcac
     9301 accctgctct ccaaacaatg gacgctgccg ccattcctgc cgaaacggct gctgctgtcc
     9361 ctgctgatac tgctggcccc caatgcaatg ttttgggttt tggcactgct gaccgccacc
     9421 gcccgcccga ttgtcaattt ggactatctt cccgccgcgc tgctgatcgc cctgccttgg
     9481 cgtttcgtca aaattgccgg cgtattggcg ttttggctgg cggttttgtt tgacgggctg
     9541 atgatggtga tccagctctt cccttttatg gacctcatcg gcgccatcaa cctcgtcccc
     9601 ttcatcctga ccgcccccgc cccttatcag ataatgaccg ggctgttgct gctgtatatg
     9661 ctggcgatgc cgtttgtgtt gcagaaagcc gccgccaaaa ccgacttccg gcacattgcc
     9721 gtctgcgccg ccgttgtggc ggcagccggc tatttcaccg gccatttgag ttactacgac
     9781 cggggtcgga tggccaatat cttcggcgca aacaacttct actacgccaa aagtcaggcg
     9841 atgctctaca ccgtcagcca gaatgccgac tttattaccg ccggcctggt cgatcccgtc
     9901 ttcctcccct tgggcaatca acagcgtgcc gccacgcatc tgaacgagcc gaaatctcaa
     9961 aaaatcctct ttatcgtcgc cgaatcttgg gggctgccgg ccaatcccga acttcaaaac
    10021 gccacttttg ccaaactgct ggcgcaaaaa gaccgttttt cggtttggga aagcggcagt
    10081 tttcccttca tcggcgcgac ggtcgaaggc gaaatgcgcg aactgtgtgc ctacggcggt
    10141 ttgcgcgggt tcgcactgcg ccgcgcgccc gacgaaaaat ttgcccgctg cctccccaac
    10201 cgtttgaaac aagaaggtta cgccaccttt gcgatgcacg gcgcgggcag ttcgctttac
    10261 gaccgcttca gctggtatcc gagggcgggc tttcaagaaa tcaaaaccgc cgaaaacctg
    10321 atcggtaaaa aaacctgcgc cattttcggc ggcgtgtgcg acagcgagct gttcggcgaa
    10381 gtgtcggcat ttttcaaaaa acacgacaag ggactgtttt actggatgac gctgaccagc
    10441 cacgccgact atcccgaatc cgacattttc aaccacaggc tcaaatgcac cgaatatggc
    10501 ctgcccgccg aaaccgacct ctgccgcaat ttcagcctgc acacccaatt cttcgaccaa
    10561 ctggcggatt tgatccaacg ccccgaaatg aaaggcacgg aagtcatcat cgtcggcgac
    10621 catccgccgc ccgtcggcaa cctcaatgaa accttccgct acctcaaaca ggggcacgtc
    10681 gcctggctga acttcaaaat caaataacaa caatgccgtc tgaacgcacc aacagccttc
    10741 agacggcatt ttgcagacag accgaccctt caagcccact tttttcatca tctccgataa
    10801 attg
//
LOCUS       DAWHRA010000070        10497 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWHRA010000070 DAWHRA010000000
VERSION     DAWHRA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.070"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..804
                     /gene="cobA"
                     /locus_tag="WIB30_001293"
     CDS             <1..804
                     /gene="cobA"
                     /locus_tag="WIB30_001293"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ3797358.1"
                     /translation="GFVSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLV
                     RRDAERIFVGKRARGDRTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETL
                     ARHQIPFSVVPGITAAVGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARS
                     RQTLVIYMGALKAALIAERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAET
                     APNPALIVIGEVVGLHEKLAWFGENGEGENRVGQAYPALGGLNAGQRAA"
     gene            815..1555
                     /locus_tag="WIB30_001294"
     CDS             815..1555
                     /locus_tag="WIB30_001294"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ3797359.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            1593..2516
                     /gene="cysD"
                     /locus_tag="WIB30_001295"
     CDS             1593..2516
                     /gene="cysD"
                     /locus_tag="WIB30_001295"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ3797360.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPAEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            2555..3841
                     /locus_tag="WIB30_001296"
     CDS             2555..3841
                     /locus_tag="WIB30_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ3797361.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKEAIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ENLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVEQTVRIEPNG
                     LTAKVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARRYLLKHGTQTLPAKVGEIESVLDVLTLEQEAGAESLKINDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            3838..5652
                     /locus_tag="WIB30_001297"
     CDS             3838..5652
                     /locus_tag="WIB30_001297"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ3797362.1"
                     /translation="MSGHDMQNTNPPLPPMPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLKAAGIQVRRAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLIERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTVPEGRYSKADPFPAALLANQKITARQSDKDVRHIELDLSGSDLSYQ
                     AGDALGVWFDNNPALVGEILDLLGIDPATEIQAGGKMMPVARALSSHFELTQNTPAFV
                     KGYAAFAHYEELDKIIADNAVLQDFVQNTPIVDVLHRFPASLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDKSKPIVMIGSGTGIAPFRAFLQQRAAENATGKNWLFFGNPHFARDFLYQTEWQ
                     QFAKEGFLHKYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            5679..7448
                     /gene="cysI"
                     /locus_tag="WIB30_001298"
     CDS             5679..7448
                     /gene="cysI"
                     /locus_tag="WIB30_001298"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ3797363.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEEFARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            7621..9480
                     /gene="ilvD"
                     /locus_tag="WIB30_001299"
     CDS             7621..9480
                     /gene="ilvD"
                     /locus_tag="WIB30_001299"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ3797364.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEQWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(9543..9803)
                     /locus_tag="WIB30_001300"
     CDS             complement(9543..9803)
                     /locus_tag="WIB30_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ3797365.1"
                     /translation="MTKAVRRKNVQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            10126..>10497
                     /locus_tag="WIB30_001301"
     CDS             10126..>10497
                     /locus_tag="WIB30_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ3797366.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFIS"
BASE COUNT         2564 a         3099 c         2881 g         1953 t
ORIGIN      
        1 ggttttgtgt ctttggtcgg cgcgggtccc ggcgatgcgg ggctgctgac gctcaaaggg
       61 ctgcaggaaa tccagcaggc agacgtggtg ctttacgacg cgctggtttc agacggcata
      121 ctgtccctcg tccgccgcga tgcggaacgg atttttgtcg gcaagcgcgc ccggggcgac
      181 cgcaccccgc aagaggacac caatgcgctt atggtcaggc tggcccgcga aggcaggcgc
      241 gtggtgcgtt tgaaaggcgg agaccctttc gtgttcggac gcggcggcga agaattggaa
      301 accttggcac gccatcagat tccgttttcg gtcgtccccg gcattaccgc cgccgtcggc
      361 gcgaccgcct atgccggtat cccgctcacg caccgcgatt atgcccaaag tgcggttttc
      421 gtaaccggcc accgcaaggc ggatgcgccc gacatcgaat ggcagaccct cgcccgcagc
      481 cgccagacct tggtgattta tatgggtgcg ctcaaagccg ccctgattgc cgaacggctg
      541 caacagcacg gacgctcgcc cgacacgccg gcagccgtca tcagccaagg cacgctgccg
      601 gctcaaaaaa ccgcaaccgg cacgctcgcc aaccttgccg aactcgccga aaccgcgccg
      661 aatccggcat tgattgtcat aggcgaagtg gtggggctgc acgaaaaact cgcctggttc
      721 ggtgaaaacg gcgaaggaga aaaccgggtc gggcaggcgt atcccgcatt gggcggactg
      781 aatgccggac aacgggcggc ataaggcggg aaaaatggaa acgacactgt tcaaacccgc
      841 cctgtggcag ataccgcata tcggatccgg cggcgaaacc gcccttgcgg aaaaaacgga
      901 aaccctcaaa caacgcctgc accggattgt cggcagccac cgggatgcac ggtttgccag
      961 cagccttgcg gcagaagata tggtgattac cgaccttatc gccggcgaaa acctcaatat
     1021 cggcattttt accttggata cggggctgct ccacacggaa accctgaacc tgctggacag
     1081 gctcggacgc gcatatcccc atttgcggat aaagcgtttc cggccggttc gagaagatgc
     1141 cgaccgttat gtggaaagca aaggcaggtt tgccttttac gacagcgttg aagcgcgccg
     1201 cgaatgctgc cgcatccgaa aaatcgaacc gctcaaccgc gccattgcag gcgcggacgc
     1261 ctggcttacc gggcaacgcc gcgaacaatc cgccacacgc acggaactgc cgtttgccga
     1321 atacgatgcc ggacggggca tcggcaaata caacccgatt ttcgactggt cggaacacga
     1381 cgtgtgggca tacatcctcg ccaacaatgt gccttacaac gatttgtacc ggcaaggatt
     1441 tcccagcata ggctgcgacc cctgcacccg ccccgtcaag gcgggcgaag acatccgcgc
     1501 cgggcggtgg tggtgggaag gcagaaacag caaagaatgc gggctgcaca aataattcat
     1561 aaatatcaga cagaagaaat aaggaatatg gtatgacgaa aaccgaaccg aacaacgccc
     1621 aactcgactg gttggaatcc gaatccatcc acatcatccg cgaagtggcg gcggaatgcg
     1681 aaaaccccgc cctgctcttt tccggcggca aagattccgt ggtcctgctc gccctcgcct
     1741 gcaaagcctt ccggctgggc agcaggccgg taaaactgcc cttcccgctg gtgcatatcg
     1801 acacggggca caactacccc gaagtgattg ctttccgcga cgcgcaggcg gcaaaactca
     1861 atgcccgcct gatagtgggg cgcgtggaag actccattgc caaaggcaca gtagttttac
     1921 gcaaagaaac cgattcgcgc aatgcggcac aggcggttac cttgttggaa accatcgagg
     1981 caaacggctt tgacgcgctg atgggcggcg cgcggcgcga cgaagaaaaa gcccgcgcca
     2041 aagaacggat tttctcgttc cgcgacgagt tcggacaatg ggatccgaag gcgcaacgcc
     2101 ccgagctgtg gtcgctgtac aacaccaggc tgcacaaagg cgaaaatatg cgcgtcttcc
     2161 cgatttccaa ctggacggaa ctcgacatct ggcaatacat cgcccgcgaa aacctcgagc
     2221 tgccgccgat ttattacagc cacaggcgcg aagtggtcag acgcaggggg ctgctggtcc
     2281 ctgtaacgcc gctcaccccg aaaatgccgt ctgaaacctc cgaaatcctt gatgtccgct
     2341 tccgcaccgt cggcgacatc agctgcacct gcccggtaga aagcaccgcg tccacgccgg
     2401 cggagattat cagggaaacg gcggttgccg acatttccga acgcagcgcg acccggctag
     2461 acgatcaggc aagcgaggcg gcaatggaaa aacgcaaaaa agaaggctat ttctaaattt
     2521 caaaaaccct ttaagcagat atgaggaaga aaatatgacg gcacaacacc aggccccgct
     2581 ccgcttcatt accgccggca gcgtcgatga cggcaaaagc accctgatcg gacgactgct
     2641 ctacgacagc aaagccctgc tgtccgacca aatcaaaacc ttggaatccg gcaaaagcaa
     2701 aggtttgaaa gaagccatag acttctccat cctgaccgac ggactggaag ccgaacgcga
     2761 acagggcatt acgatcgatg tggcataccg ctatttttcc acggcgaaac ggaaattcat
     2821 catcgccgac acgccggggc acgaacaata cacgcgcaat atggttacgg gcgcaagcac
     2881 cgcttcggcg gcagtcgtgc tggttgacgc atcccaactg gatttcggcg cgcagccctt
     2941 gcagcttctg ccgcagacca aacgccattc cgccatcctc cgccaactca actgcccgca
     3001 tattgtggtg gcggtcaaca aaatggacct gctggattac agcgaagaca aattcaacgc
     3061 cattgtggag gcataccgcc gacttgccga acagctcggt ttgaaagacg tgcatttcgt
     3121 accggtgtcc gcactcttgg gcgacaacat cgtttacccc ggcggcaata tgccttggta
     3181 taaaggcgaa cccttattgt ccattttgga aaacctgccg ggggcggacg aagtcagccg
     3241 caccgccgac gatttttatt tccccgtgca actggtcgtc cgccaagatg ccgacaaggc
     3301 cgatgatttc cgaggctatc aggggcgcat cgaacgcggt tcggtcgccg tcgaacaaac
     3361 cgtccgcatc gaaccgaacg ggctgaccgc caaagtgtct gaaatcatca cacccaaagg
     3421 cgaagtggcg caggcttttg ccggcgaggc ggcaaccctc cgactggatc gcgacatcga
     3481 tgtttcgcgc ggcgaccttt ttgtcgataa aaattccccg ctcgccccgc aaaaacatct
     3541 ggaagccacg ctttgctggt ttgacgaacg tccgctcaac accgcgcgca gatacctgct
     3601 caagcacggc acgcaaaccc tgccggcaaa agtcggggaa attgaaagcg ttttggatgt
     3661 cctcacgctg gaacaagagg caggcgcgga atccttgaag ataaacgaca tcgccaaagt
     3721 ccgcatcaac cttcaaaaac ccgttacggc aacgccttat gcggaaaaca ccgccgccgg
     3781 ttcgtttatc ctgatagacg aagcgacata cggcactgtt gcggcaggta tgattttatg
     3841 agtggacacg atatgcagaa cacaaatccg ccattaccgc ctatgccgcc cgaaatcacg
     3901 cagctcctgt cggggctgga cgcggcacaa tgggcgtggc tgtccggcta cgcttgggca
     3961 aaagcaggaa acggggcatc tgcaggacta cccgcgcttc agacggcatt gccggcggca
     4021 gaaccctttt ccgttaccgt cctttccgcc tcgcaaaccg gcaatgcgaa atccgttgcc
     4081 gacaaagcgg cggacagcct gaaagccgcc ggcatacaag tcaggcgcgc cgaactgaaa
     4141 gactacaagg cgaaaaacat cgcagacgaa cgccgcctgc tgctggttac gtccactcag
     4201 ggcgaaggcg aaccgccgga agaagccgtc gtgctgcaca aactgctgaa cggcaaaaaa
     4261 gccccgaaat tggacaaact ccaatttgcc gtactgggct tgggcgacag ctcctatccg
     4321 aatttctgtc aggcaggtaa agatttcgac cggcgttttg aagaattggg cgcaaaacgc
     4381 ctgatcgaac gcgttgatgc ggatttggac tttaccgcct ctgcaaacgc ctggacagac
     4441 aatatcgccg cactcttaaa agaagaagcc gcaaaaaacc gggcaacacc cgcgccgcag
     4501 gcaacgcccc ccgccggcct tcagacggta ccggagggca ggtactctaa ggcagacccc
     4561 tttcccgccg ccctgctggc caatcagaaa atcaccgccc gccagtccga taaagacgtg
     4621 cgccacatcg aacttgattt gagcggttcg gatttaagct atcaggcagg cgacgcgctc
     4681 ggcgtttggt ttgacaacaa tccggcactg gtcggggaaa tcctagacct gctcggcatc
     4741 gatccggcaa cggaaataca ggcaggcgga aagatgatgc cggttgcgcg cgcactttca
     4801 tctcatttcg aactcacgca aaacaccccg gctttcgtca aaggctatgc cgcgttcgcc
     4861 cattatgaag aactcgataa aatcattgcc gataacgccg ttttgcagga tttcgtgcaa
     4921 aacacgccta ttgtcgatgt gctgcaccgc ttcccggcaa gcctgacggc agaacaattc
     4981 gccggcctgc ttcgcccgct tgcgccgcgc ctgtattcga tttcctcgtc gcaggcggaa
     5041 gtgggggacg aagtgcacct gaccgtcggc gcggtgcgtt ttgaacacga agggcgcgcc
     5101 agggcgggcg gcgcatcggg tttccttgcc gaccggctgg aagaggacgg cacggtgcgt
     5161 gtatttgtgg agcgcaacga cggcttcaga ctgcccgaag acaaaagcaa acccattgta
     5221 atgataggct ccggtacggg cattgcaccg tttcgcgctt ttttgcaaca acgcgccgca
     5281 gaaaatgcaa caggcaaaaa ctggctgttt ttcggcaatc cgcattttgc ccgtgatttt
     5341 ctctatcaaa ccgaatggca gcagtttgcc aaagagggtt tcctgcataa atacgatttc
     5401 gcctggtccc gcgatcagga agaaaaaatc tatgtgcagg acaaaatccg cgaacaggcg
     5461 gaaggacttt ggcaatggct gcaggaaggc gcgcatatct atgtgtgcgg cgatgcggca
     5521 aaaatggcaa aagacgtgga agccgccttg ctggatgtga ttatcggggc aggacatttg
     5581 gacgaagagg gcgcagaaga atatttggat atgctgcgcg aagaaaaacg ctatcagcgt
     5641 gatgtttatt gattaaatat aatcgggagg aacacaaaat gaccgtacag accaagacaa
     5701 aaggtttggc gtggcaagaa aaaccgctat ccgacaacga acgtctgaaa accgaaagca
     5761 attttttacg cggcacgatt ttggacgatt tgaaagaccc gctcacgggc ggcttcaaag
     5821 gcgacaactt ccaactcatc cgcttccacg gtatgtatga gcaggacgac cgcgacatcc
     5881 gcgccgaacg cgccgaggca aaactcgagc ccttgaaatt tatgcttttg cgctgccggc
     5941 tgccgggcgg gatcatcaaa ccgtcccaat ggatagaact ggacaaattt gcccgggaaa
     6001 acagtcatta ccgctccatc cggctgacca accggcaaac cttccaattt cacggcgtgc
     6061 cgaaagccaa gttgcagacg atgcaccgcc tcttgcacaa actgggtttg gattccatcg
     6121 ccacggcggc ggatatgaac cgcaacgtgc tttgcacgtc caacccgatc gagtccgaac
     6181 tgcaccggca ggcttacgaa tacgcgaaaa agatttccga acacctgctg ccgcgcacgc
     6241 gcggttatct ggatgtgtgg gtggacggca aaaaagttca aagttccgac gacttccttc
     6301 aagaagacga gccgattttg ggcaaaacct atctgccgcg aaaattcaaa accgcagtcg
     6361 tcatcccgcc cttgaacgat gtggactgct acggcaacga tttggatttc gtcgccgttt
     6421 cagacggtaa cggacagctt gccggcttca atgttttggc aggcggcggg ctttcgatgg
     6481 aacacggcaa caccaaaacc tatccgaaca tttcactgga actgggtttc gtgcctccgg
     6541 aacacgcgct gaaggccgcc gaagcggtgg taaccacgca gcgcgacttc ggcaaccgca
     6601 gcgaccgcaa aaacgcccgc acccgctaca ccattcaaaa tatgggcttg gacaacttcc
     6661 gcgcggaagt tgaacgccgt atgggtatgc cgttcgaacc cgtacgcccg ttcaaattta
     6721 ccgggcgcgg cgaccgcatc ggctgggtga aaggcataga cggcaactgg catttaaccc
     6781 ttttcatcga aagcgggcgt ttggttgacg aaggcgggaa acagcttctg accggcgtgt
     6841 tggaaatcgc caaaatccac aaaggcgatt tccgcatcac cgccaaccaa aacctcatcg
     6901 tggcaaatgt cgccgaagca gacaaagcaa aaatcgaaga atttgcccga acatacggat
     6961 taatccgcaa cgatgtcagc aagctgcgtg aaaatgcgat gtcctgcgtt tcctttccga
     7021 cctgcccgct ggcaatggcg gaagccgaac gcgtgctgcc ggacttcatc ggcgagctgg
     7081 ataagattat ggcgaaacac ggcacgtcgg acgactacat cgttacccgc attaccggct
     7141 gcccgaacgg ctgcggacgg gcgatgttgg cggaaatcgg actggtcggc aaagccgtcg
     7201 gacgctacaa cctccatatc ggcggcgacc gtgaaggcgt acgcatcccc cgtctttaca
     7261 aagaaaacat caccctgccc gaaatccttg ccgaattgga cgacctgatc ggcaaatggg
     7321 cggcagaacg caatatcggc gaaggtttcg gcgatttcgc catacggacg ggcatcgtca
     7381 aacccgtatt aaatgcaccc gttgattttt gggacgcatc caaagccgtc gcgattgccc
     7441 gcgcctgaac ccgtccccgc attcctgcgg ggattttctt ttttgccgca cgccgtttca
     7501 atcccgcaaa attccgatac ttggtataaa ataccctctt ttcccactct aaaaaaccgt
     7561 acccgatacc gtctgaaaca gccctgtctt tcagacggta taggcacaag gaaacacact
     7621 atgcctgaat accgctccaa aacctccacc cacggccgca atatggcggg cgcacgtgca
     7681 ttgtggcgcg ccaccggcgt gatggaaacc gacttcggca agcccatcat cgccgttgcc
     7741 aactccttca cccaattcgt gcccggccat gtccacctgc acaatatggg ccagctggtt
     7801 gcccgcgaaa tcgaaaaagc cggcgcaatc gccaaagaat tcaacaccat cgccatcgac
     7861 gacggcatcg ctatgggaca cagcggcatg ctgtactccc tgcccagccg cgatttgatt
     7921 gccgactcta tcgaatatat ggtcaacgcc cactgcgccg acgcgctggt gtgcatttcc
     7981 aactgcgaca aaatcacccc gggcatgctg attgccgcga tgcgcctgaa catccccacc
     8041 atcttcgtct ccggcggccc gatggaagcg ggcaaggtta tcggcgtggc aaacatccag
     8101 cccgaacgcc gtttggactt gattgacgcg atgattgaat cggcggacga caatgtcagc
     8161 aaccggcaag tcgaggaagt cgaacaaaac gcctgcccga cctgcggctc gtgttcgggt
     8221 atgtttacgg caaactcgat gaactgcctg accgaagcac tcggcctttc cctgcccggc
     8281 aacggttcgt atttggcgac ccacgccggc cgcaaagaat tgttcctcga agccggccgt
     8341 atgattgtcg aaatcaccaa acgctattac gagcaaaacg atgaaaccgt gttaccgcgc
     8401 agcattgcca ccaaaaaagc gtttgaaaac gctatgacga tggatattgc gatgggcggc
     8461 agcaccaata ccattttgca cctgttggcc gttgccaacg aagccggtgt cgatttcaaa
     8521 atggcagaca tcgaccgctt aagccgcgtc gtgccctgca tctgcaaaac cgcacccaac
     8581 aaccacgact actatatgga agacgtgcat cgcgccggcg gtatcttcgc catcctgaaa
     8641 gaactggaca aagcgggcaa actgcacacc gacgtgcaca ccatccacgc gccgacgctg
     8701 aaagacgcga ttgaacaatg ggacgtgacc aatcccgaaa acacccgtgc catcgaacgc
     8761 ttcaaagccg cgccgggcgg cgtacgcacc acccaagcgt tctcacaaaa ccgtatgtgg
     8821 aaaaccctcg acctcgaccg cgaaaaaggc tgtatccgcg acgtggcaca cgcctactcg
     8881 caagacggcg gtttggcggt cttgttcggc aacatcgccg agcgcggctg cgtggtaaaa
     8941 accgcaggcg tggacgagag catcctcaaa ttcaccggcc gcgcccgcgt gtttgaaagc
     9001 caagaagacg cagtagaagg cattttgggc aaccaaatcg tcgctggcga catcgtcatc
     9061 atccgctacg aaggcccgaa aggcggcccg ggcatgcagg aaatgctgta tccgacttcc
     9121 tacctgaaat ctaaaggcct cggcaaagcc tgcgctctct taaccgacgg acgcttctcc
     9181 ggcggcacat caggtttgtc catcggacac gcctcgcccg aagcggcgga aggtggcgcg
     9241 atcggtttgg tacacgaagg cgataccgtc gaaatcgaca tccccaaccg cagcatccac
     9301 cttgccattt ccgatgaaga gcttgccgca cgccgtgccg aaatggaagc gcgcggcagc
     9361 aaagcatgga agcctaaaaa ccgcgaccgc tacgtctccg ccgcattaag agcttacggc
     9421 gcgatggcga cttccgccga caaaggcgcg gtgcgcgacg tagcgcaaat cgaaagataa
     9481 gccccaaaac gtgcggcaaa tgctgtctcg tctgaacagt aaaggtctga tgatggacaa
     9541 atttatctgg gcgatggcgg tattttccgc aattttggcc tcgttatcgg cagcggcttt
     9601 agcgcgatcg agtatgtaaa agagctgccg ctcgccgccg ttgccgtgat gccttgcgcg
     9661 ccgaagtcgt tgctgtggtt gttggcgaag ctgaggtagt cgaatcccgc gtcggcaagg
     9721 tattgcccgt atgcggaggg cgttcggaat gcatagcata ttttgggggt ttgcacattt
     9781 tttcggcgta ccgccttcgt caaacagcgt gccttcgagg ttgccgacgg taatgtccgc
     9841 gtcttgcaag gcagattcga cgtttttcag aatattggta tcgggcaggt aatcgaccgg
     9901 ataattgctg ccgagcataa tgtcgcccac gccgataatg gaaacggtat cggctgtctt
     9961 ttcgtcaggc gcatcctgtc cgtccacatc gccggtattt gaaacgggcg cggggtttga
    10021 accgtcaatc ggcagcacgg ggattccgtt ccggcaggat gcgccgaggg cttgaccccg
    10081 gcggtttgcg gtgcggaaac ggcataaagg gcgaaccttg acgaattgaa catcaaatat
    10141 tcgggcatcg aaaacaggtt tgaaaccgcc atcctgaaga aaaacggcgt aagatccggc
    10201 ttcgtctcct tcgcccccaa ccttgccgcc gtcaaactga acgattatgc caaagttaga
    10261 aaacggatta ccaaaaccaa acagaaagcc gacatcgtca ttgtgatgtt ccacggcggc
    10321 gcggaaggga aacaggcgga acacctgccg ttcgataccg aaatcttcta tggggaaaac
    10381 aggggcaacg tcgttgagtt tgcgcggctt gccgtcgatt ccggcgcgga tgtcgtattc
    10441 gggcaggggc cgcacgttac tcgcgccgtc gaactttacc gcgaccgctt catctcc
//
LOCUS       DAWHRA010000071        10489 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWHRA010000071 DAWHRA010000000
VERSION     DAWHRA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.071"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            27..572
                     /locus_tag="WIB30_001302"
     CDS             27..572
                     /locus_tag="WIB30_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ3797367.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMHNIVASILRNNY
                     GLTVRTDGEGKGNMPLREAVKLIRGSDVAIEFHTNAAASKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAAKV"
     gene            569..742
                     /locus_tag="WIB30_001303"
     CDS             569..742
                     /locus_tag="WIB30_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797368.1"
                     /translation="MKKSLIALALSVLKPQMPEFEIKPANIGYLKQHPSMRPGKSGVS
                     AAKRAARKRKNRR"
     gene            745..909
                     /locus_tag="WIB30_001304"
     CDS             745..909
                     /locus_tag="WIB30_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797369.1"
                     /translation="MGQVAFYEKMIGLWSSKSREASERADLAAFEFAEGELANYREML
                     KRHLQTKSVE"
     gene            912..1148
                     /locus_tag="WIB30_001305"
     CDS             912..1148
                     /locus_tag="WIB30_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797370.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            1120..1539
                     /locus_tag="WIB30_001306"
     CDS             1120..1539
                     /locus_tag="WIB30_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797371.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQAVID
                     KMERDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNASGGCIDGFGSHGLQLYNRALGYGN"
     gene            1559..1741
                     /locus_tag="WIB30_001307"
     CDS             1559..1741
                     /locus_tag="WIB30_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797372.1"
                     /translation="MPTPPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQN
                     QAWRDWAGNHSRKVGN"
     gene            complement(1786..1956)
                     /locus_tag="WIB30_001308"
     CDS             complement(1786..1956)
                     /locus_tag="WIB30_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ3797373.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            1969..2187
                     /locus_tag="WIB30_001309"
     CDS             1969..2187
                     /locus_tag="WIB30_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797374.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            2184..2522
                     /locus_tag="WIB30_001310"
     CDS             2184..2522
                     /locus_tag="WIB30_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797375.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            2534..2800
                     /locus_tag="WIB30_001311"
     CDS             2534..2800
                     /locus_tag="WIB30_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797376.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKADAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            2797..2994
                     /locus_tag="WIB30_001312"
     CDS             2797..2994
                     /locus_tag="WIB30_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797377.1"
                     /translation="MSDPILDALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            2997..3503
                     /locus_tag="WIB30_001313"
     CDS             2997..3503
                     /locus_tag="WIB30_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ3797378.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(3500..4090)
                     /locus_tag="WIB30_001314"
     CDS             complement(3500..4090)
                     /locus_tag="WIB30_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797379.1"
                     /translation="MNWLNRGIQEKILRVLSDNYPNCLKATKVYNSLFPSSSLPITSN
                     IPVDKQGLGLALTLGCEIQSQHLATLQSDEFQYFLKNIYYLEESGLIEITSIDSLHKN
                     FDCKINHKGIDFLTDDGGLSAILGVVTVKLHSDTIQALITAKIDQAEISDSEKSWLKK
                     ELGKIKDTALSTLTENAINAIPAATLITLLKKFVGL"
     gene            4103..5725
                     /gene="terL"
                     /locus_tag="WIB30_001315"
     CDS             4103..5725
                     /gene="terL"
                     /locus_tag="WIB30_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ3797380.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTK
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYQANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRVTG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            5725..7293
                     /locus_tag="WIB30_001316"
     CDS             5725..7293
                     /locus_tag="WIB30_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ3797381.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEECDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     NGTTATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPVNRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            7280..8575
                     /locus_tag="WIB30_001317"
     CDS             7280..8575
                     /locus_tag="WIB30_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ3797382.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            8685..9101
                     /locus_tag="WIB30_001318"
     CDS             8685..9101
                     /locus_tag="WIB30_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ3797383.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            9332..10393
                     /locus_tag="WIB30_001319"
     CDS             9332..10393
                     /locus_tag="WIB30_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797384.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFDLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAKGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
BASE COUNT         2757 a         2458 c         2915 g         2359 t
ORIGIN      
        1 caatcaatat tttttggaga tgatgaatgg gcaaaaccgt aaccttaacc gctggacaca
       61 gcaacaccga cccgggtgcg gtcaacggca gcgaccgtga ggcggacttg gcgcaggata
      121 tgcacaacat tgtggcttca atcctgcgta acaattacgg cctgaccgta cgcaccgacg
      181 gcgaaggcaa aggcaatatg ccgcttcgcg aagctgtaaa actgattcgc ggctcggatg
      241 tggcgattga gttccatacc aacgcggcgg ccagtaaagc agctacgggc attgaggcgt
      301 tgagtaccgt taaaaacaaa cgctggtgtc aggtgttgag caaagctgtt gccaagaaaa
      361 ccggctggaa actgcgcggc gaagacggct ttaaacccga caatgcgggc cagcattcgc
      421 gcctagctta tgcacaagcc ggcggcattg tgtttgagcc gttttttatc agcaacgaca
      481 ctgatttagc cttgtttaag actactaaat ggggcatctg ccgcgcgatt gcggacgcga
      541 ttgcgatgga attgggggcg gcgaaggtat gaaaaagtct ttgattgctt tggctctgtc
      601 tgtcttgaaa ccgcagatgc ctgaatttga gattaagcct gccaatattg gctatttgaa
      661 acaacatccg tctatgcgcc cgggtaagtc gggcgtgtcg gcagccaaac gcgcggcgcg
      721 taaacgcaag aatcgtcgtt aattatggga caggttgcgt tttacgaaaa gatgattggg
      781 ctgtggtcga gcaaaagccg tgaggcaagc gaacgggctg atttggcggc gtttgaattt
      841 gcggagggcg aactggccaa ttatcgggaa atgctgaaac ggcacctgca aaccaaaagt
      901 gtggaatagc aatgcgtatt ttggatattt ttaaaaaccc cgcgacaggc aatgtgtcgc
      961 actcgaaact gtgggcaaac gttgcctgcg cggcggggac gtttaagttt gtgatgctgc
     1021 ccgatccgtc ggcggagatt tgggcggtgt atttgggcat tgtcggcggc tatgcggtgg
     1081 cgcgctcgtt tgtcagcgtc aaacgtcagg aggtcgagaa tgaatctcgt gaaactgctg
     1141 gcgaataact ggcaaccgat tgccatcatc gcgcttgtcg gcacgggctt ggcggtgtcg
     1201 caccatcaag gctacaagtc ggcatttgcg aagcagcagg cggtcatcga caagatggag
     1261 cgcgacaagg cgcaagccct gctgttgtcg gctcaaaact atgcgcgcga actggaacag
     1321 gcgcgtgcgg aagcgaaaaa atatgaagtc aaggcgcacg ctgtcggcat ggctttggcg
     1381 aaaaaacagg cggaagtcag ccgtctgaaa acggaaaata aaaaggaaat cgaaaatgtc
     1441 cttactcaag accgtaaaaa tgcaagcggc ggttgcattg acggctttgg ctctcacggc
     1501 ctgcagctct acaaccgcgc cctcggctac ggaaattaag gttgtcgaaa aggcagtcat
     1561 gccgaccccg cctgctgcat tgatggtcgc gccggtacgc ccgaatccgc cgaaagacgg
     1621 caagacggcc acgctgttgg aacacgccgc tgagtttggt ggctatgtgg cggagttgga
     1681 aaatcaaaat caggcttggc gcgactgggc gggcaatcac tcccgcaaag tcggaaactg
     1741 acaaaaaagc ccgcgtaggg cgcgggctga gggtgaaagc ggattttata cctcttttac
     1801 aggggtagcg gcggtagtgc ttttcagcaa atcgactgcg tgctgacagt tttgcttgct
     1861 ggtgtagcct tcgccctgag cgatgatttc atggttggct gctttcaaac gccaacggta
     1921 ttcgcctttt gcgtctttat agatttcaaa atacataagg tttctcctat gaatgagtac
     1981 acgttttctt accgctttaa cggcaagtcc tggtcattga gcatttgggc ggacaaccct
     2041 gaagaagcca gggcgaaatt tcgggctgca cgagaaaatg cgcactatga cggcgaagtt
     2101 gtagcaaagg tttatacatt tgtaaatatt tcgtgggtta agaaattgta caagcggaca
     2161 aaatatttaa tgggtatcaa agaatgacct accgtgaatt agttgaacgt cagttggctg
     2221 tgcgccatgc cgatttggaa ttgggcttaa gccgcgcacg cgagcaagag ccgtttgtca
     2281 ttcatgtttc cgatctgttg gataaggcag gcattgagta cgcggtacgc atggataagg
     2341 attttcagac gacgtttcac cttgaatatc caattacgaa ctatgacacc tttaaacgtg
     2401 cggtttggca aactttgggg gcgtattact gtgtttgtaa tgatggtgat ggactggaga
     2461 ttgccagcaa tcgccctgac ggttacgccg tccgtatcgt attcggcgat gtgccggttt
     2521 aaaggggttt taaatggact ttgaatttgg tttcagaacc ctgtggccga ttgcgacggc
     2581 ggcattttgg ttttgggtca acggcatttc aggccgcctg aaagaggcgg acaagcgtat
     2641 cgacgacctt aaagaggagt tgcacgcggt caagctctct tatcacacca aggcggacgc
     2701 caaggcagac agcactaata ttgcggcggc cttggagcga attgaaaaca agttagaaaa
     2761 agtaaacgaa aaactggaca ggaaagcaga caaatcatga gcgacccgat tttggatgcc
     2821 ttggcgcgta ttgaaaacaa gactgatcaa acgctgaaaa atcagaagga aatgcaggcg
     2881 gaaattgcgc aaattcgcca agacacgaaa cgcacggcca ttacattcgg cgcactgggc
     2941 ggcggcgtga ttacggtcgg ctgggaattg cttaaagcga aaatgggact gtaattatgg
     3001 ctcacccgca agaaatccgt gaaaagttac gccggctcta tgtgagcggc gagcaaactt
     3061 tggaaacggc ggccttgatg tgcgaaatcc cgcagaccac tgcgcgtgcg tggaaacgtg
     3121 cggataagga aaaaggcgac gactgggata agatgcgcgc cgcttacact ttggccggcg
     3181 gcggtattga ggatttgagc cgtgcgatgt tggccggttt tatggtgcag tacaacagca
     3241 cgatgacgat gctgcaggat tcgagtaccg aagatttgcc accatccgac cgcgccaagc
     3301 tgttggccag cctggccgat gcgtttacga aaaccgtatc ggccaatgcg cgtgtgatgc
     3361 cggaaacgtc aaaactggcg acggctttgg aattgattga gttcttgatg gcgtttgtac
     3421 aagaaaaaca tcccaaacat ttgcctgcct ttgtggaggt attggagccg tttggggtgg
     3481 aagtggagaa gaagtttggt tagaggccaa caaatttttt taaaagagta atgagggtgg
     3541 cggcagggat agcattaatt gcattttccg taagcgtact taatgcagtg tccttgattt
     3601 tccctaattc ttttttcaac cagctttttt ctgaatcaga aatctctgct tggtctattt
     3661 ttgccgtaat taaagcctga atagtgtcgc tgtgtagttt gactgtgaca acaccaagaa
     3721 tggcggatag gccgccatca tcggtaagga agtctatgcc cttatgatta attttgcagt
     3781 caaaattttt atgaagtgaa tctatcgaag taatttcaat taaaccagat tcttctaagt
     3841 aataaatatt ttttaaaaaa tattggaatt catctgattg taaagttgct aggtgttgac
     3901 tttgaatttc acaaccaaga gtcaaagcca agcccaatcc ttgtttatca acagggatgt
     3961 tagaagtaat aggtaaagaa ctactaggga aaagagagtt atacaccttg gttgctttta
     4021 ggcagttcgg gtaattatca cttaaaactc gtaagatttt ttcctgaata cctctattta
     4081 accagttcat aaattattcc tcatgaaaac aaaagaattc ctcaaatccc ttgccgaact
     4141 ggccgccagt ttgcgccaag tcatcgaagc ggaagtggac ggcttcgatg cgtcgcccaa
     4201 ggctattgct gcacgccgtg ccaaggtgtt tgacccggta ggcggttacg agtatttcgt
     4261 gaatacctac ttcccccatt atatccgctc gcctgagaaa tccgaactgc atgcgttttt
     4321 attcagccgt ctgccggaga ttatccgctc ccccaaaggg gaaaatgagg cggtgggtgc
     4381 gccgcgtgga gagggtaagt cgacgaaggt tactcagttg tttacgctgt ggtgtattgt
     4441 gaccggccaa aaacattatg ctgttattgt gatggacagt atcgaccagg catatccgat
     4501 gctggaagcc atcaaggcgg aacttgaatt taacccgcgc ttgaaaaccg actttccgga
     4561 agtatgcggg cagggccgtg tatggcaggc cggtacgatt gtgacggcca atgacgttaa
     4621 agtccaagtg gccggtagcg gtaaaaagct gcgcggtttg cgtcacggcc cttaccgtcc
     4681 tgacttaact gttttggacg atattgagaa tgacgagcaa gtccgcaacc ccgaacagcg
     4741 cgacaagctc aatgcgtggc tgactaagac cgtattgcct ctgggcggtg tcggtcagaa
     4801 atacgatgtg atttatatcg gcacgatttt gcattacgac agcgtactta accgcacttt
     4861 gaataacccg ttttggcacg gtattaagtt taaggcgatg aaacgctggc ctgaccgcat
     4921 ggatttgtgg gacaggtggg aggaactttt ccgaaacgac ggcgagacgg tggccgaggc
     4981 gttttatcag gcaaacaaag acgagatgga gcgcggcgcg gtcacttctt gggcggcgcg
     5041 tggcgtactc gcgctgatga aaatccgtgc gcgtgacggc catgcgacgt ttgattcaga
     5101 atatcaaaac gatccggtca gtggcgaaga tgcgccgttt gccaagtcga tgaagttttg
     5161 gaacgacctg ccgtccgatt tggtgtattt cggtgcgctc gacccgtcac tcggaaaggc
     5221 cggggcgagc cgtgacccgt ccgcgattat cattggcggt tatcaacgtg taaccggcaa
     5281 actgtatgtc gtggaggctc agattaaaaa acgtctccct gatttgatta ttgaggacgt
     5341 tattcgattg caccgtcaat atcgttgcaa actgtggttt gttgagactg ttcaatttca
     5401 ggaatttctg aaagacgagc tggtcaagcg cagcgcggcg cgtggaatac ctgtcccagc
     5461 gcgggcggtc aaaccggtat cggacaagct gttgcggatc gagactttac agcctcacat
     5521 ggcgaacggt ttgattctgt tgaatgagag ccaacaaacg ctgatacagc agttccgcca
     5581 ttttccaaag gctgatcatg atgatggtcc tgatgccgtg catatgctct ggtcgggggc
     5641 ggtggccaat tgtgtgccga tagaatggca aagccctacc gataacgatt ttgatgacga
     5701 gataaaaagt aaatggagcc gataatggca aaaaagaaca ataaaactaa aatccaaaag
     5761 cccgaagctg cattgcagac ggacgtggct caaattacgg cgaccggtcg ggttatcgcc
     5821 gagcatccgt ccaattttat tacgccgcaa aagatgcggg ccctcttcga ggacgcagaa
     5881 agcggcgaca tccgcgccca acacgagctt ttcgcggaca ttgaggagtg cgacagcgac
     5941 atcgcggcaa atatggggac gcgcaaacgc gcgctgctga cgctcaactg gcgcgtcgcc
     6001 ccgccgcgaa atgcgacgcc cgaagaagaa aagctgtccg accaagccta cgaaatgatg
     6061 gacagcctgc ctaccctcga agacctgatt atggatttga tggacgcggt agggcacgga
     6121 ttttctgcgt tggaggtcga gtgggtattt tcagacggcc tttacctacc ccgaaacttt
     6181 atccaccgcc cgcaaagctg gttcaaatgg gacaaagaca acgggctgct gctgcgtacc
     6241 cgcgaaaatc cggaaggcga agcgttgtgg ccgctgggct gggtcgttca tacccaaaaa
     6301 tcgcgcagcg tccagcaggc gcgcaacggg cttttccgca cgctttcctg gctgtatatg
     6361 ttcaaacact acgccgtcca cgattttgcc gagtttttgg agctgtacgg catgcccatc
     6421 cgtatcggca aatacggcgc gggcgcaact aaagaggaaa aaaacaccct gcttcgagcg
     6481 gtggcggaaa tcggtcacaa cgcggcaggc atcatgccag aaggtatgga aatagagctc
     6541 cacaacgcgg caaacggtac gacggcaacc agcaatccgt ttttgcagat ggccgactgg
     6601 tgcgaaaaat cggcggcgcg gctgattttg gggcaaacgc tgaccagcgg tgcggacgga
     6661 aaatccagca ccaacgcgct gggcaatatc cacaacgagg tacgccgcga tttgctggtg
     6721 tcggacgcaa aacaggtggc gcaaaccatc acaagccaaa tcatcggacc gttcctgcaa
     6781 atcaactatc cccatgccga cccaaaccgc gtgccgaaat ttgaatttga cacgcgcgag
     6841 ccgaaagaca tcgcggtctt tgccgacgct atcccgaaac tggtggatgt cggcgtacaa
     6901 atccccgaaa gctgggtgcg cgacaaactg gtcattccag atgtgcagga gggtgaggct
     6961 gtgttggtgc ggcaggtacc ggacaatccg gtaaacagaa ctgcattggc ggctttatcc
     7021 gcccacaccg taccatctaa ggctacgggc aggcatcagg aaatattgga cggcgcgttg
     7081 gatgacgcgc tggttgagcc cgatttcaat tctcagctca acccgatggt gcgtcaggcg
     7141 gttgccgcac ttaatgcttg caacagctac gaggaggcag atgccgcact gaatgcgctt
     7201 tatccgaatt tggacaacgc gaaactgcgt acctatatgc agcaggcctt gtttatcagc
     7261 gatattttgg gacaagacca tgcccgcgcc tgatttggga tttgccttaa gtctgccgcc
     7321 aaaaaaggca atcgagtggc tggaaagtaa aaaggttacg gcggagagct accgcaatct
     7381 gacagcctcc gaaattgcca aagtctatac gattgcccgc atgaccgact tggatatgct
     7441 caacgacatc aaaacttcga tggttgaatc ggcaaaaagt ggacagtcgt ttgacgattg
     7501 gcgaaaaggt atcttgaatc tgctcagcaa caagggctgg ctgcatccga acgggcataa
     7561 cggtaaggat atcatcgacc cagccaccgg cgaggtattc ggttcgccgc ggaggttgga
     7621 gacgatttac cgtaccaata tgcaaactgc ctacaacgcc ggtcaatatc aaggatatat
     7681 ggcaaatatt gatgcacgac cttattggat gtatgacgcg gtaggcgaca gccgcacccg
     7741 tccggcgcat tcggcaatag acgggctggt gtaccgctac gacgacccgt tttgggcaac
     7801 gttttacccg cccaacggct acaactgccg ctgctcggtc atcgcgctgt cggagcggga
     7861 tgtggaacgc caggggcgga ttgttgggca aagcacggcg gacaatctgg tcgagaccca
     7921 taaaatctac aacaaaaaag gcgatactta tctgaccctt gcctataaag caccggatgg
     7981 cagtctgtac acgaccgatc gaggatttga ttacaacgcc ggacgaatga actaccgccc
     8041 cgatttagac aagtacgacc gtgcgttggc gcatcaattt gccaaagcgg aaatgggtgg
     8101 tgcggatttt aaaaccagct ttaaacagct tgaaaaagag ttttatgaag tcaagcaacg
     8161 tttggatatt gatggcaagc ccgataaaga gcagaaaatc aaaatccgaa atgcgctatc
     8221 aagacagctt aaatttgctg cgggtgtatt gagcaaggaa acgcaagaat tggcaggtat
     8281 gacacgagcg acggtgtggc tgtctgatga tacgttggtt aaacaggtag acagccgtga
     8341 ggggcagaat ttcgatgact cctactatgc ttttttgccg gatatgctgc aaaaccctga
     8401 acatgtcatc cgcgacaatc gtgaattgat tttcacagct cgctataaag gctcggcatt
     8461 gtgggcagtt ttaaaatata ttaaggaggt ggatgagatt tatctacagt cgtaccgaat
     8521 cagtaacgac aaagagattg ccaaatttat ggcgaagaag aaagtattga aatagacgtt
     8581 gggcaaggct cgaaatcact tgcacacgct ctcggacgcc ctaacgggca ggctgcggta
     8641 tcgaggttat caccgctttt ccaacgtctg tacggaataa taccatgatt gatgtcaaaa
     8701 tagacaatat ctttgtcgtc ctaaaccaaa tcgagcggct tggcaacggg atcgaaaacc
     8761 gctacctgct gatgcgccga ctgtccgaaa ccatgcacac ggcggtcaag ctcaatttcc
     8821 gctacgcagg ccgtccgaaa tggttggggc taaaataccg cgacggcaag ccgctttcgg
     8881 attcgggtcg tctgaaagac agtttttcca cactgtcaga caacgataca gcccttgtcg
     8941 gtacgaatat cgtctatgcc gccatccaca acttcggcgg tatggcgggg cgcaaccgca
     9001 aagttcggat tccgcaacgg gaatttttga cgctgacgga cgacgacaaa caggctttga
     9061 tggacgatgt gcaggattat ttttcgggtc tgataccgtg aatttataaa accctcaaaa
     9121 acgcgctttt tagcgcgttt ttttatgcgg gtaatacaaa cccctgccca agatataaaa
     9181 atcaatccta gacgcttcta aaaagcccct gaaaacgatt aattgtgtat cgcgcggaca
     9241 ggttttaaaa aaatggcggg agggtttgaa gcacgcctac tctttgttgt tttttcaaat
     9301 aggcaaaatg accgtattga gagaggtaca catgtccaaa aatgcacaaa aaaccctact
     9361 tgccgtgtgc agtttcgagg tgcagccaaa agacgggcga atccaactgc tgccatatgg
     9421 cgaatttcgc gcagtagacg gtcgtccgac tgatgtccct gcgtggtatc tgaccgaaga
     9481 aaacggtcat gatgtcgcgt tgttggccaa cagctcgcgc aatcagttgg ttgtcgatta
     9541 tgaacaccag acgctctaca aagagaaaaa cggacaacct gcacctgccg ccggttggat
     9601 gcgttggctg gagttcacgc ctaaaggcat gtttgccgaa gtggagtgga cggacaaggc
     9661 ggctgcggca attgccgcaa aagagtatcg ctacatctct gctgtgtttt cctatgacac
     9721 aaagggatat gtaagcaaaa tttttcacgc cgcgctgaca aatttccccg cgttggacgg
     9781 tatggacgag gtgctggcgg cagcgtcggc gcaaatttta aaaccggaaa cggagcaaaa
     9841 ccctatgaaa gagttgttac agcaactgtt cgacctgcct gatgcgggcg aagaagaact
     9901 gaaggcggca ttgtccgcgc tcgtggaagc caagccgaaa gacgtggcat tgtctgccga
     9961 cgtgttcgcg cagctggcgg aaaaagacag ccgcatcgcg gcattgacgg cgcaaaccgc
    10021 caagcctgat ttgactaaat acgcgcctat ctcagtggtt caagagctgc aaagcaaagt
    10081 cgccgcgctg actgccaagc aggaagcaga caaaggcaac gaattgatta ccgccgcgct
    10141 gacttcaggc aaattgctgc ctgctcagaa ggagtgggca aaaggcgtat tgaaacagcc
    10201 gggcggcttg gcatttttga ccggctttat tgaaaacgcc cagccggtcg ctgcactggc
    10261 aggctcgcaa acgggcggca aagcacccga cgaacgcgtc gccgcactga ctgcggaaga
    10321 ggcagccgca gcaaaaatgc tgggcatgtc cggcgaagaa tttgtaaaaa tcaaagaaag
    10381 cgaaggtaag taatggacaa atcagcgatt ttgaccgcaa tcacggcagc attccgcaaa
    10441 gaatttcaaa cggccttgga ttcggatttc aagtgcaaca ctaaggtac
//
LOCUS       DAWHRA010000072        10271 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWHRA010000072 DAWHRA010000000
VERSION     DAWHRA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.072"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(84..1187)
                     /locus_tag="WIB30_001320"
     CDS             complement(84..1187)
                     /locus_tag="WIB30_001320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ3797385.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1267..2823)
                     /gene="dnaA"
                     /locus_tag="WIB30_001321"
     CDS             complement(1267..2823)
                     /gene="dnaA"
                     /locus_tag="WIB30_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ3797386.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VSASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3115..3249
                     /gene="rpmH"
                     /locus_tag="WIB30_001322"
     CDS             3115..3249
                     /gene="rpmH"
                     /locus_tag="WIB30_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ3797387.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3252..3617
                     /locus_tag="WIB30_001323"
     CDS             3252..3617
                     /locus_tag="WIB30_001323"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ3797388.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3682..3903
                     /gene="yidD"
                     /locus_tag="WIB30_001324"
     CDS             3682..3903
                     /gene="yidD"
                     /locus_tag="WIB30_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ3797389.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4076..5713
                     /gene="yidC"
                     /locus_tag="WIB30_001325"
     CDS             4076..5713
                     /gene="yidC"
                     /locus_tag="WIB30_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ3797390.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMVAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(5794..6507)
                     /locus_tag="WIB30_001326"
     CDS             complement(5794..6507)
                     /locus_tag="WIB30_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ3797391.1"
                     /translation="MVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQSVVSKTVADWRR
                     MKEMPNLKKRPTIFVMYAG"
     gene            complement(6566..7156)
                     /gene="maf"
                     /locus_tag="WIB30_001327"
     CDS             complement(6566..7156)
                     /gene="maf"
                     /locus_tag="WIB30_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ3797392.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAHKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7304..7807
                     /locus_tag="WIB30_001328"
     CDS             7304..7807
                     /locus_tag="WIB30_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ3797393.1"
                     /translation="MSDPNLIDSEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            7841..8020
                     /gene="rpmF"
                     /locus_tag="WIB30_001329"
     CDS             7841..8020
                     /gene="rpmF"
                     /locus_tag="WIB30_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ3797394.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8195..8722
                     /locus_tag="WIB30_001330"
     CDS             8195..8722
                     /locus_tag="WIB30_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ3797395.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            8811..9866
                     /gene="plsX"
                     /locus_tag="WIB30_001331"
     CDS             8811..9866
                     /gene="plsX"
                     /locus_tag="WIB30_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ3797396.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            10002..10253
                     /locus_tag="WIB30_001332"
     CDS             10002..10253
                     /locus_tag="WIB30_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ3797397.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
BASE COUNT         2602 a         3096 c         2440 g         2133 t
ORIGIN      
        1 ttccgacagc cgaacaaaac cccgaatccg tctgcacggt tcggggcata tctccaatac
       61 gggcatcgtg ctccggaaaa ccgtcaaatt cgcatcggca tcacgatata tttgaaattc
      121 ggattgttcg gcacggtaaa cagcgtcgag cggttggcat cgccgaaggc aagctgcata
      181 tcgtcggaat ggatgttgcg caacacgtcc atcagatagc cgatattgaa accgacttcg
      241 agttcgccgc cctgataggc aatctcgatt tcttcgcgcg cttcttcctg ctcgttgttg
      301 ctgcacacga cgctcaacag gccggattgc aggaacaggc gcgcgccacg gaatttttcg
      361 ttggcaagaa tggacacacg ttccagcgcg cccaaaagtt cggcacggga caaaacgaaa
      421 atcttgtcgt tgtccaaagg aatcacgcgg ttgaaatcgg ggaatttgcc gtcgatgacc
      481 ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct gtttgtccag cagctcgatt
      541 tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc
      601 aaaatcactt cggcgcgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg
      661 tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg
      721 agataatagc ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg
      781 aaacgctcct gctccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga
      841 aaatcggcgg caggcagggt ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga
      901 cggctgtcgt cccaatccag cgacaccagc gcaccggcag gcagtgcccg taaaatgtcc
      961 tgaaatttct tggcattggt ggtgatgcgg aagtcgtccg cgccgccctc gggacccgca
     1021 gtgtcgattt ggatttccaa atcggttgcc aagagtttgg tctgaccgcc ttttccctca
     1081 atcaggacgt tggacaggat gggcagggta tggcggcgtt cgacgatgcc ggtaacggct
     1141 tgcagcggct tgagcaggct gtcgcgttcg gcttgtaaaa tcaacatttt cgctccttaa
     1201 atcggttgga ttttttaccc ttcggacggc atgggcaata tcatgccgtc tgaaaggcgc
     1261 gttcggtcag ttttgaatca gaatcagcag tttctcgtaa tcctgcgcca actcggggtc
     1321 ttcctcgcgc agtttcgcca ctgccctgat gccgtgcata acggtcgtat ggtcgcgccc
     1381 accgaacgaa tcgccgatag acggcaggct caaagtggtc agttctttgg tcaggctcat
     1441 cgccacctgg cgcggacggg caatgtttcg tgtccgtttc ttaccgagca catcgctgat
     1501 tttgatgcgg taatatttcg caacggcatc aataataata tcggcggtga tgactttgtg
     1561 cttctcggca ataatgtcct gcaaagcggt acgcgccaaa tcgatgtcga tgacgggacg
     1621 gttcataaag cggctgctcg cgctgacgcg gttgaacgcg ccttcaagct cacgcacgtt
     1681 ggaacggatc agattggcaa tgaacagcgc ggcttcgtct tcaatactga tgcccgccgc
     1741 ttccgccttt ttctgcaaaa tggcgatacg catttccaat tcgggcggct cgagttccaa
     1801 agtcagtccc catgaaaaac gggatttgag gcggtcgtcc ataccttcga ttttggcggg
     1861 taaaacatcg caagtgagga tgagctgttt tttctcgtta tggaaatgat tgtacagata
     1921 gaagaattcc tccatcgtac ggtctttgcc tttgatgaac tggatgtcgt cgataatcag
     1981 caggtcgtat tgcttgtatt gctgcttgaa cacgtcgtag gtattgttgc gaaccgcctt
     2041 cataaagctg cggatatagt cgtccgaatg catatagcgc actttggcat cgggacggtt
     2101 tttcaacagt tcgttgccga ccgcctgcac aaggtgggtt ttgcccaaac ccgtactgcc
     2161 atagaggaag aacgggttgt aactctgccc cgggctttcc gcaatcgcct gcgccgcagc
     2221 cgccgcaagg cggttgccct taccttctac caacgtatca aacgtgtaat ccggagacag
     2281 gttggtctgc tcgtaacgcg cctcttccgc atcgcgctgc acgtccgtcc gtgctttggc
     2341 aactgccacc gattccggcc gggaagcaga cccggcagcc tgacgcggct cgtgcggcag
     2401 gtttttcata cgttccgcca aaatatccgc cgccgttttc gacgcagcgg gtttgacagg
     2461 ctcttcagac ggcagctcgt ccaacagaac ctcctgcacg ggcattccct ccgacaccgc
     2521 atgcaaggac ggctcggcag gttcgacagc accttcaacc gccgccatct cataacgcac
     2581 gccttctccc ggtttgaata cgaaggcgga acggccggca gccaactctt ccctcaccgc
     2641 ttctattttt ccggcaaact ggctcttgag catattgcag gcaaactggt tcttgccgta
     2701 caccacccat acgccaccct cctcaccaac ggtaaggggc gcaatccatt gcgcaaactg
     2761 cccttgaggc aacatatcgt gaagacggcg gaggcacagc ggccaaaact ctgctaatgt
     2821 catggatagg ctcgaatcgg taaaaatgaa atcgaaaaca aagaaaatat aatattttca
     2881 aaaagaaaac aaatctgttg aacgcacatc ggttcaaaac gcgactgccc gattataccg
     2941 actcacgaat attttatcca caacccgtgc aaaaatttat ccacagaaag gcggcggaaa
     3001 tccgcaggca atcgggcaat cttcctgcaa agtttctata ttgattgaca aaagcggcaa
     3061 attggagtgt aattcacggt ttaattatct acccattcta ttttaggaaa catcatgaaa
     3121 cgcacttatc aaccttccgt taccaaacgc aaacgcaccc acggcttcct ggtgcgctcc
     3181 aaaacgcgcg gcggccgcgc agtattggcc gcacgccgtg ccaaaggccg caaacgcctg
     3241 gcggtataat tttggactac cgcttcggaa ggcagtaccg cttgttgaaa acggatgatt
     3301 tttcatccgt ttttgcattc agaaaccgcc gcagccgcga cctgctgcaa gtttcgcgct
     3361 caaacggcaa cgggctgggc catccccgca tcggtctggt ggtcggcaaa aaaaccgcca
     3421 aacgcgccaa cgaacgaaat tatatgaagc gcgttatccg cgactggttt agattgaaca
     3481 aaaaccggct gccgccgcag gatttcgtcg tgcgcgtcca ccgtaaattc gacagggcta
     3541 ccgcaaaaca ggcaagggcg gaactggcac aactcatgtt cggcaacccc gcaaccggat
     3601 gcaggaaaca ggcatgatta gaacggtact ctgcaggcaa ggttcagacg gcaacgggtt
     3661 tcccatacaa ggaacatccc gatgaacttt ctattgtcca aactcctgct gggactgata
     3721 cggttctacc aatattgcat cagcccgctg attccgccgc gctgccgtta tacgccgacc
     3781 tgttcgcaat acgcggtcga agcggtcaaa aaatacggcg cattcaaagg cggccggctc
     3841 gccatcaagc gcattgcacg ctgccaccct ttcggcggac acggacacga ccccgttccc
     3901 tgacccgacg caatattcaa attgcacgct ttccttttat ttcccatcgg tttctatata
     3961 atgccgtctg aaacttcggg ctggcggcac gaccgtcggg tatgaagccc gcccttattc
     4021 cccgtctatc ggaacacgca acctgcggca tttccgacca ttcaggaaac tctatatgga
     4081 ttttaaaaga ctcacggcgt ttttcgccat cgcgctggtg attatgatcg gctgggaaaa
     4141 gatgttcccc actccgaagc ccgtccccgc gccccaacag gcagcacaac aacaggccgt
     4201 aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac
     4261 ggttcaagcc gtcattgatg aaaaaagcgg cgacctgcgc cggctgaccc tgctcaaata
     4321 caaagcaacc ggcgacgaaa ataaaccgtt catcctgttt ggcgacggca aagaatacac
     4381 ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctaa aaggcatcgg
     4441 ctttagcgca ccgaaaaaac agtacagctt ggaaggcgac aaagttgaag tccgcctgag
     4501 cgcgcctgaa acacgcggtc tgaaaatcga caaagtttat actttcacca aaggcagcta
     4561 tctggtcaac gtccgcttcg acatcgccaa cggcagcggt caaaccgcca acctgagcgc
     4621 ggactaccgc atcgtccgcg accacagcga acccgagggt caaggctact ttacccactc
     4681 ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca gcttctccga
     4741 cttggacgac gatgccaaat ccggcaaatc cgaggccgaa tacatccgca aaaccccgac
     4801 cggctggctc ggcatgattg aacaccactt catgtccacc tggatcctcc aacccaaagg
     4861 cggacaaagc gtttgcgccg ctggcgactg ccgtatcgac atcaaacgcc gcaacgacaa
     4921 gctgtacagc accagcgtca gcgtgccttt agccgccatc caaaacggcg cgaaagccga
     4981 agcctccatc aacctctacg ccggcccaca gaccacatcc gttatcgcaa acatcgccga
     5041 caacctgcaa ctggccaaag actacggcaa agtacactgg ttcgcttccc cgctcttctg
     5101 gctcctgaac caactgcaca acatcatcgg caactggggc tgggcgatta tcgttttaac
     5161 catcatcgtc aaagccgtac tgtatccatt gaccaacgcg tcctaccgtt cgatggcgaa
     5221 aatgcgtgcc gccgcgccca aactgcaggc aatcaaagag aaatacggcg atgaccgcat
     5281 ggcgcaacaa caggcgatga tgcagcttta cacagacgag aaaatcaacc cgctgggcgg
     5341 ctgcctgcct atgctgttgc aaatccccgt cttcatcgga ttgtattggg cattgttcgc
     5401 ctccgtagaa ttgcgccagg caccttggct gggttggatt accgacctca gccgcgccga
     5461 cccctactac atcctgccca tcattatggc ggcaacgatg gtcgcccaaa cttatctgaa
     5521 cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc
     5581 cgtcatgttc ttcttcttcc ctgccggtct ggtattgtac tgggtagtca acaacctcct
     5641 gaccatcgcc cagcaatggc acatcaaccg cagcatcgaa aaacaacgca cccaaggcga
     5701 agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc agacggcatt
     5761 tttattgttc accccctatc ggggcggaaa tcttcaacct gcatacatca caaaaatcgt
     5821 cgggcgtttt ttcagattgg gcatttcctt catcctgcgc caatccgcaa ccgttttact
     5881 gaccaccgac tgagtcggca acgtcaaatc cgtagccgtg cacaagcgcg tttcaggatg
     5941 caggttttcc accgcatccg ccaacaacac atcgttgcga tacggcgttt cgataaacag
     6001 ctgcgtttca ttctgctggc gcgaacgttg ttccaaagcc ttcaaactct gaatccgctc
     6061 atttttttca gacggcaaat aacccttaaa cgcaaagttc tgcccattcg cacccgaagc
     6121 catcagcgcc agcaacaagc tggacggacc gaccaaaggc cgtacttcaa aaccgtgttt
     6181 atgcgccaat gccaccaaat tcgcacccgg atcagccaca gccgggcaac ccgcctcgct
     6241 gacaatgccc atactgcgcc cttcttgcaa aggtttcagc aattccggca aagtcttcaa
     6301 atccgtgtgt tcgttcagcg tttgcaaatt cagttcgcgg ataggcattg tcacgcccaa
     6361 atgtttcaaa tgcgcgcgcg ccgttttttc cgcttccacg acaaaatccg tcagcccgac
     6421 aatcgcctgt tgttcatgcg gcaacaggca cggcgtgtca ggcgtaccca aaggcgtagg
     6481 aatcaaatac aaaacaggag acaccattcc ctcactcatc ggttaaaaat gccgtctgaa
     6541 cccttcagac ggcataaacg ggcagttaca gaacctccac gccctcattt ttcaaaaaat
     6601 caaccagacg gaaaaccggc aaaccgatta aagcattcgg atcggtactc tcaatccttt
     6661 cgatcaacaa tgcacccaaa gcctcgctct tcaacgcaca cgagcaataa accgcatcag
     6721 gctcgcgctc caaatagcgg aggatgtgta gctcgtccaa cttcctcatc acgaccaccg
     6781 tcttatcgat atgccgctgc atcctgcccg taaccgtatt tagcagaacg accgcgctgt
     6841 aaaactcaat ctccctgccg ctcaaatgca tcaacatctt gtgcgcatta gcaaggttca
     6901 tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg
     6961 cctctgggaa acgcccggcc aacgaccgcg ccttaccctc ggcaaggcgc aatgccgtct
     7021 gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggacgcc gcctgaaagg
     7081 caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc aaaatcaaag
     7141 gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag gggcagtaaa
     7201 atccagccat gcgccgaaac acggataccc gccttcggcg taccgcaaca tttttcttaa
     7261 aaatattgac gttagaacat ctaaattata tcatatcccg tttatgtcag accctaattt
     7321 gattgactcg gaaatttttg ccgccgaagg gcagaacctg caaggcagtt ttctgctgga
     7381 agaattggat gaacgcgtca gttcgcacga ttatcccgcc gacaggcaga ccaaaatatc
     7441 gtttacactg accggcggtc gcgaccggct gcaacgcctg ttcctcgacc tgaacgtcaa
     7501 agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca tgcttgatga
     7561 aagcagccgt atcgtcctgt tttccaacga agagtccttg gacgaatcca tgcttgccga
     7621 cgaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcacat tggtagaaga
     7681 ccaaatcctg atgtccctgc ccttttcgcc gcgacacgaa gactgcggcg acaatgggac
     7741 actggaagaa gtcaatcggg acaaacccaa cccctttgct gttttggcag gtttgaaaag
     7801 caattgatta ggacacagtt tatttatcta ggagcttgaa atggccgttc aacaaaacaa
     7861 aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcgctgaccg cgcccgcact
     7921 gtctgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatttccc ccaacggtat
     7981 gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg actgactgaa
     8041 aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca ccatccgtcc
     8101 aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataatac cccgtgaaaa
     8161 tattccaaaa gccccaacca ccaaggaaat tccgatgaaa cagaaaatct ggtacaccta
     8221 cgatgacatc caccgcgtca tcaaagcatt ggcagaaaaa atccggaacg ccgacatcaa
     8281 atacgatgcc atgattgcca tcggcggcgg cggctttatt ccggcacgta tgctgcgctg
     8341 ttttctggaa attccgattt atgccgtaac caccgcctat tacgacagcg acaacgaagg
     8401 acaggttacc gaagaagtca aaaaagtcca atggctcgac cccgttcccg aagccctgcg
     8461 gggcaaaaac gtactcgtcg tcgatgaagt ggacgacagc cgcgtaacca tggagttctg
     8521 cctgaaagaa ctgctcaagg aagacttcgg tacgatcgga gtcgccgtac tgcacgaaaa
     8581 aatcaaagcc aaagcaggca aaatccccga aggcattccc tatttcagcg gcatcaccgt
     8641 agaagactgg tggatcaact atccgtggga cgcactcgac atcgacgaac acaaccgcct
     8701 tgccgaggcc ggccgaggct gaccctttca gacggcatat tttccgaacc gatgccgtct
     8761 gaagcccgca cgacccctgc cgcagaccga aaacctaccg gagaaaccct atgattacat
     8821 tggccgtaga tgccatgggc ggcgaccaag gacttgccgt taccgtaccc ggcgcaaccg
     8881 cattcctcca agcacacccc gatgtccgcc tgattatgac cggcgacgaa acgcaactgc
     8941 gccaagccct gaccgcggca ggcgcaccga tggaacgcat cgacatctgc cataccaccc
     9001 aagtcgtcgg catggacgaa gccccgcaat ccgccctgaa aaacaaaaaa gactcctcca
     9061 tgcgcgtcgc catcaaccag gttaaagaag gcaaagccca agccgccgta tccgcaggca
     9121 acacgggtgc gctcatggca accgcacgtt tcgtcctcaa aaccattccc ggcatcgaac
     9181 gccccgccat cgccaaattc cttccttccg acaccgacca cgttaccctt gcactcgacc
     9241 ttggcgcgaa cgtcgactgc acgcccgaac agctcgccca atttgccgtt atcggcagcg
     9301 aactcgtcca cgcactccat cctcaaaaag gacagccgcg cgtcgggctg gtcaacgtcg
     9361 gcacggaaga catcaaaggt acggacaccg tcaaacaaac ctacaaactg ctgcaaaaca
     9421 gcaaactcaa ctttatcggc aacatcgaaa gcaacggcat cctctacggc gaagcagatg
     9481 tcgtcgtcgc cgacggcttt gtcggcaacg tcatgctcaa aaccatcgaa ggcgcggtca
     9541 aattcatgag cggagccatc cgccgcgaat tccaaagcaa cctgttcaac aaacttgccg
     9601 ccgttgccgc cctacccgcc ctcaaagggc tgaaaaacaa actcgatccg cgcaaattca
     9661 acggggccat cctgctcggg ctgcgcggca tcgtgattaa aagccacggc ggcacagacg
     9721 aaaccggttt ccgctacgca ctcgaagaag cctaccacga agccaagtcc gccggccttt
     9781 ccaaaatcga acagggcgta gccgaacaac tcgccgcact cgaaactgcc aaagccgtcc
     9841 aaaacgaaaa tgtcggcggt ctgtaacacg cgcgatgccg tccgaacgcc cccgcccctt
     9901 tcagacggca tccgcccgcc ccgaacctgc gggcgcggac ggcgatgcac ctgtccggca
     9961 cttcccaaat acttccctgt aaaataagga atatttgaaa aatgaagaca ttagaaaaac
    10021 ggatgaaagc tctagacaaa cggatgatga agttcggaaa atcccttgaa ggcaggcttg
    10081 atgcccgtct gattgaatcc gcattggatt atattcatta ttcggaacgt tttttggctt
    10141 ttgaaatcct gtgtacttat atcgaagatt tcgatgtccg gctgacggaa caagaatccc
    10201 gggaaatttc ttttatcaac aaggaatttg agatagaaag cacgtccgat taaccaataa
    10261 agccaatggg t
//
LOCUS       DAWHRA010000073        10172 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWHRA010000073 DAWHRA010000000
VERSION     DAWHRA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.073"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WIB30_001333"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WIB30_001333"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ3797398.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVNADYAVKVPDGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1457..1846)
                     /locus_tag="WIB30_001334"
     CDS             complement(1457..1846)
                     /locus_tag="WIB30_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ3797399.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFATYDKNKLKQNEYFKITLSEVSPDHFTLQAAPDPMTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2072..3250
                     /locus_tag="WIB30_001335"
     CDS             2072..3250
                     /locus_tag="WIB30_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ3797400.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3316..5250
                     /locus_tag="WIB30_001336"
     CDS             3316..5250
                     /locus_tag="WIB30_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ3797401.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5667..6449)
                     /locus_tag="WIB30_001337"
     CDS             complement(5667..6449)
                     /locus_tag="WIB30_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ3797402.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6588..8777
                     /locus_tag="WIB30_001338"
     CDS             6588..8777
                     /locus_tag="WIB30_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ3797403.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGIV
                     WETDIAPDMDTARILSVQTAFMEEKPLPQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSYHGQASHSEFVLNADQQKASDEIQTALGRFRPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESVSRQDLHRAVSLWVQVLQQNRDSKIRWSVDVDPQEA"
     gene            complement(8823..9897)
                     /locus_tag="WIB30_001339"
                     /pseudo
     CDS             complement(8823..9897)
                     /locus_tag="WIB30_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(9945..>10172)
                     /locus_tag="WIB30_001340"
     CDS             complement(9945..>10172)
                     /locus_tag="WIB30_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ3797404.1"
                     /translation="AVLKALYMATVAATRFEPLIRDFYQRLLSEGKPYKVAVTVCMRK
                     LLTISNARMRDYFAENDTAENDTAENGIRTA"
BASE COUNT         2362 a         2617 c         2973 g         2220 t
ORIGIN      
        1 gacgtgttgc gataagggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtactgcca
      121 cgtcaaatca gtggtgatgg ccgcagccgc attctttttt catatcgatt accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggcacaacc aaaccttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagcccga tggcaaccac acgtccgccc gcgcggacgc
      421 aattcacggc agagttgaat gcggcagcag atacggcggt tacgaccgca gcgtgtgcgc
      481 cgccggtttt ttcctgaatc actttggcag cgtcttcttt ggcggcgttg acaaccaaat
      541 ccgcgccggt ttctttggca aacgccagtt tgtcgtcgtt gatgtcgatg gcgacaacgt
      601 gcgcgccgaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacac tggaaacttt aatggcttta taagtggtca
      721 cgccggcgca agtgatgctg gacgcttggg cggggtcgag accgtcgggg actttgaccg
      781 cataatccgc attgacgatg cagtgggtcg ccataccgcc gtcggcggtg tagcccgcgt
      841 tcaatacaga gcggcacagg gtttcgcggc cggtattgca gtattcgcaa gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtattccaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catctcacag
     1261 gtcaagtggt atgttgttga aaaatataga ttatatgtta tattataaca gcttggaaag
     1321 gcgcggcatc ggggcggttg ccggatgagg ggcggcaggt ttcaagtttg aaaaaccgga
     1381 cggcaaaccc gtaaagatac cgtctgaagc tgtgtccgga cggcatcttt acgggtttgc
     1441 gggcttcggc ggaggattag tcgaagccgg ggcaggattg gtttgtaccg gaagcggcaa
     1501 tggtaccgcc gtcgttgagc gtaacgacgc aggtttcgcc gtcgttggtc atcggatcgg
     1561 gggctgcctg aagggtgaag tggtcggggc tgacttcgct taaggtaatc ttgaaatatt
     1621 cgttttgttt cagtttgttt ttgtcgtagg ttgcaaacgt ccctttttgg cggtagtaac
     1681 gttccatggt ctgcgcgttg tgcagcaggg tcgtcctgac ttccgacagg cggacgcgcc
     1741 ggatgtaggt tttataggaa gggtaggtga tgagcgtcag gatgccgagg atggcgacgg
     1801 caatcatcag ctcgagcagc gtaaagcctt tttgaacgtt tttcatagca atgtgtttcc
     1861 atttgtttgt cggtcgacgg cattataacg ccgtatcgga aatggcggaa tatgtaaacg
     1921 gattgaaatt ttcgggaaag cagattgtat aagccattta aaacaaatgg ttatttttat
     1981 tgtcggcagt ttgccgcctt ggatggggca gggacttgcg gtagaatccg cttccgattt
     2041 atgggattga cgcatacaga gaattgaaaa catggcaaaa atgatgaaat gggcggctgt
     2101 tgcggcggtc gcggcggcag cggtttgggg cggatggtct tatctgaagc ccgagccgca
     2161 ggctgcttat attacggaaa cggtcaggcg cggcgacatc agccggacgg tttctgcaac
     2221 aggggagatt tcgccgtcca acctggtatc ggtcggcgcg caggcatcgg ggcagattaa
     2281 gaaactttac gtcaagctcg ggcaacaggt taaaaagggc gatttgattg cggaaatcaa
     2341 ttcgacctcg cagaccaata cgctcaatac ggaaaaatcc aagttggaaa cgtatcaggc
     2401 gaagctggtg tcggcacaga ttgcattggg cagcgcggag aagaaatata agcgtcaggc
     2461 ggcgttgtgg aaggatgatg cgacttccaa agaggatttg gaaagcgcgc aggatgcgtt
     2521 tgccgccgcc aaagccaatg ttgccgagct gaaggcttta atcagacaga gcaaaatttc
     2581 catcaatacc gccgagtcgg aattgggcta cacgcgcatt accgcaacga tggacggcac
     2641 ggtggtggcg attctcgtgg aagaggggca gactgtgaac gcggcgcagt ctacgccgac
     2701 gattgtccaa ttggcgaatc tggatatgat gttgaacaaa atgcagattg ccgagggcga
     2761 tattaccaag gtgaaggcgg ggcaggatat ttcgtttacg attttgtccg aaccggatac
     2821 gccgattaag gcgaagctcg acagcgtcga ccccgggctg accacgatgt cgtcgggcgg
     2881 ttacaacagc agtacggata cggcttccaa tgcggtctac tattatgccc gttcgtttgt
     2941 gccgaatccg gacggcaaac tcgccacggg gatgacgacg cagaatacgg ttgaaatcga
     3001 cggcgtgaaa aatgtgctga ttattccgtc gctgaccgtg aaaaatcgcg gcggcaaggc
     3061 gtttgtgcgc gtgttgggtg cggacggcaa ggcggcggaa cgcgaaatcc ggaccggtat
     3121 gagagacagt atgaataccg aagtaaaaag cgggttgaaa gagggggaca aagtggtcat
     3181 ctccgaaata accgccgccg agcaacagga aagcggcgaa cgcgccctag gcggcccgcc
     3241 gcgccgataa acgaatatgc cgtctgaaca cggaaacggt ttcagacggc atttgttatt
     3301 gatttacgga atattatgag cttgatcgaa tgtaaaaaca tcaaccgcta tttcggcagc
     3361 ggcgagaacc gcgtccatat tttgaaagac atcagcctgt cgatagagaa gggcgatttt
     3421 gtcgccatca tcgggcagtc cggttcgggc aagtccacgc tgatgaacat actcggttgt
     3481 ttggataccg ccggttccgg ttcgtaccga atcgacggca tcgaaactgc caaaatgcag
     3541 cctgacgaac tggcggcatt gcgccgcgaa cgcttcggct ttatcttcca acgctacaac
     3601 ctcttaagct cgctgaccgc aagggataac gtcgcgctgc cagccgtcta tatgggcgcg
     3661 ggcggcaaag agcgttccgc gcgggcggac aaactcttgc aggatttggg tttggcaagc
     3721 aaagagggca acaagcccgg cgaactctcg ggcggacagc agcagcgcgt ctccatcgcc
     3781 cgcgccctga tgaacggcgg agaaatcatc ttcgccgacg agccgaccgg cgcgctcgat
     3841 accgccagcg gcaaaaacgt gatggaaatc atccataagc tgcacgaagc cgggcatacc
     3901 gtcattatgg tcacgcacga ccccggcatc gccgccaatg ccaaccgcgt catcgaaatc
     3961 cgggacggcg aaatcatttc cgacacctcg aaaaatccag aaatccccgc aagcaatgtc
     4021 gggaggattc aggaaaaagc ctcgtggtcg ttttattacg accagtttgt cgaagccttc
     4081 agaatgtcgg tgcaagcagt attggcgcac aaaatgcgtt cgcttctgac gatgctcggc
     4141 atcatcatcg gtatcgcttc ggttgtctcc gtcgtcgcat tgggcaatgg ttcgcagaaa
     4201 aaaatccttg aagacatcag ttcgataggg acgaacacca tcagcatctt cccggggcgc
     4261 ggcttcggcg acaggcgcag cggcaggatt aaaaccctga ccatagacga cgcaaaaatc
     4321 atcgccaaac aaagctacgt tgcttccgcc acgcccatga cttcgagcgg cggcacgctg
     4381 acttaccgca ataccgacct gaccgcttct ttgtacggcg tgggcgaaca atatttcgac
     4441 gtgcgcggac tgaagctgga aacggggcgg ctgtttgacg aaaacgatgt gaaagaagac
     4501 gcgcaggtcg tcgtcatcga ccaaaatgtc aaagacaaac tctttgcgga ctcggatccg
     4561 ttgggtaaaa ccattttgtt caggaaacgc cccttgaccg tcatcggcgt gatgaaaaaa
     4621 gacgaaaacg ctttcggcaa ttccgacgtg ctgatgcttt ggtcgcccta tacgacggtg
     4681 atgcaccaaa tcacaggcga gagccacacc aactccatca ccgtcaaaat caaagacaat
     4741 gccaataccc aggttgccga aaaagggctg accgatctgc tcaaagcgcg gcacggcacg
     4801 gaagatttct tcatgaacaa cagcgacagc atcaggcaga tagtcgaaag caccaccggt
     4861 acgatgaagc tgctgatttc ctccatcgcc ctgatttcat tggtagtcgg cggcatcggc
     4921 gtgatgaaca tcatgctggt gtccgttacc gagcgcacca aagaaatcgg catacggatg
     4981 gcaatcggcg cgcggcgcgg caatatcttg cagcagtttt tgattgaggc ggtgttaatc
     5041 tgcgtcatcg gcggtttggt cggcgtgggt ttgtccgccg ccgtcagcct cgtgttcaat
     5101 cattttgtaa ccgacttccc gatggacatt tcggcggcat ccgttatcgg ggcggtcgcc
     5161 tgttcgaccg gaatcggcat cgcgttcggc tttatgcctg ccaataaagc agccaaactc
     5221 aatccgatag acgcattggc gcaggattga ggttggcaca aagatgccgt ctgaagctgc
     5281 aggaccggtc attttggagc agaaacttat tggacaaaaa cggtttctta gattctacgt
     5341 tctagattcc cacttgcgtg ggaatgacgg cggcgggggt tcgatgattg cacacaacgc
     5401 tcgagtcccg tcattcccgt aaagacggga attcggttcg ttcggctttg cttgtttcgg
     5461 ataaatcacg gtaactcaat attccagatt cccgcccgcg tgggaacggc ggcggggctt
     5521 cgtattgttc aatttattat tttcaatcat tcaatgggtt aggatgtgtt tgttggcttg
     5581 ctaactttca gtgcggattg gttttcagac ggcatttccc gcaaaaaagg cttggaattt
     5641 tccaagcctt ttgttttgcg gatggattat tgatttttgc ggatgatttc ctccagttgg
     5701 ggcatcgggc tgtagccgct ttggctgcgc ccgttgggga agacgagggt cggcgtgccg
     5761 ttgaagccga attgttcgcc caaggaagtg gtttccgcga cgggattgtc gcagatgctg
     5821 ccgccgaccg ggaatttgcc tttacgcatc caatccgtcc acgctttggc gcggtcgggc
     5881 tgacaccata agatttgcgc cttgcgcgcg gcatcggggt gcaggccggc aatgggcatc
     5941 ataaagctgt aaaccgtcac gtcggtcatt ttttcaaact cgtgttccaa gcgtttgcag
     6001 aacggacaat cggggtcgga gaagacggcg actttcagct tgccgttgcc gcgcacttct
     6061 ttgatggctt tgtccaaagg cagggaggcg aagtcgattt tgttcaaatc ggcggcgcgt
     6121 tcttcggtca ggtttttgcg cgtgtcgatg ttgatgagtt cgccgacgaa catatagccg
     6181 ccttcggcat cggtgtagat aatctgcctg ccgctgacga cgacttcgta aatgcctttg
     6241 accggtgttt cgctgacgct caacactttc aaatcttggg cggaataggt tttttccaaa
     6301 cgcgctttca aagaggcggc aacggatttg ccggcggact cggctttgac ggcgggttcg
     6361 gcgttggcat tggaaacggg cgtttgcccg caagccagca gtgggaggac ggtaaagggg
     6421 gtcaagattt tgattaactt ggttttcata taaagatgat tgcgcgtgtt ggaaaagcgg
     6481 aattgtatca aatctctgtt gcgcctgcat tgcgcctagg ctcaatttat cgtctgaaaa
     6541 tagcttccgc ctgttaaaat acgcaaaaaa atgatttgcc tgcctgtatg atctaccacc
     6601 gcatcgccgt aaacgtgccg ctttcagacg gccttttgac ttattcccat tccgatccgc
     6661 ttcctccggg aacgcgggtg cttgtgcctt tccgtaataa aaccgttgtc gggattgtgt
     6721 gggaaacgga tattgcgccc gatatggata cggcgcggat tttgagtgtt cagacggcct
     6781 ttatggaaga aaagccgttg cctcaaagct ggcgtgattt gttggcgttt acgtcgcgtt
     6841 attaccacta tccgactggg caggcggtgt ttgccgcgct gccgcagggt ttgaaggaaa
     6901 cgcgcgcggt ggaaatgccg cagccgccgt tgttttatgc tttgaacgaa gcgggcaggg
     6961 cgcaaacgcc gccgccggcg cggtttaaca aaaaagcggc tttgtgggac gcgctgcttt
     7021 cgggcggaat gacgatggcg gcgttgaagc aggtaaacgc gcaggcggcg aaattgattg
     7081 aagattgggc ggaacagggt tggattgaaa cgacggaggc ggcgaaaccg gtattgaggt
     7141 cgtaccacgg gcaggcttcg cactctgaat ttgtattaaa cgccgaccag cagaaggctt
     7201 ccgatgaaat tcagacggca ttgggacgct tccggccgtt tctgctgtac ggcatcaccg
     7261 gcagcggcaa gaccgaagtg tatttcgatg cgatggcgaa agtgttggcg caggggcggc
     7321 aggtgttgtt tctgttgccc gaaatcaacc tcacgccgca gcttttaaag cgggtggaaa
     7381 accgttttgc tgacgtgccg accgccgtgt tgcacagcca gatggcggca ggcaggcgca
     7441 cgcaggatta tttgcgcgcg atgttggggc aggcgaaatt ggtcatcggc acgcggctgg
     7501 cggtgttcac gccgatggat gatgtcgggc tgattgtggt cgatgaggaa cacgacggct
     7561 cgttcaaaca ggacaacgaa ttacgctacc acgcccgcga tttggcggtg tggcgggcga
     7621 agcagagcgg ctgccccgtt gtgttgggca gtgctacgcc cagtttggag agttggcaca
     7681 aggcgcaaag cggcgcgtac cgcctgctgc aactgaccga acgcgcccat accgccgcgc
     7741 aactgccgca agtggacatc ctcaacgtag gtcgtctgaa actcgacaac ggcttctcgc
     7801 cgcaagcctt gcagcttttg aaacagaact ttgaagcagg cggtatgtcg ctggtgtacc
     7861 tcaaccggcg cggtttcgcg cccgcgctgt tttgcggcga ctgcggccat accttcggct
     7921 gcccgaactg ctccgccaaa atggtgttgc accaacgcgc ccgccaattg cgctgccacc
     7981 actgcgacca ccgcgaaccc attccgttca aatgccccga ctgcggcaac caagacctga
     8041 ccgccgtcgg acacggcacg cagcgcgtcg aagaaaccct gcgcgccttc ctgcctaaag
     8101 ccgccgtcgt ccgcgtcgat agggacagca cggcgcacaa aaatgactgg gcggatttgt
     8161 accgccgtat cgccgacaac gaaatcgaca ttctggtcgg cacgcagatg ctcgccaaag
     8221 gtcatgattt cgcgcggctc aacctcgtta tcgtattgaa cgccgatggc agcctgtata
     8281 gcgcggactt tcgtgcgccg gaaaggctgt ttgccgagct gatgcaagtg tccggcaggg
     8341 cggggcgtgc cgacaaaccc ggcaaggtgt tgatacagac ccaactgccc gaacatcccg
     8401 tcttcgccgc cgtcaaggcg caggattatg ccgtgtttgc cgaaaatgaa ttgaacgagc
     8461 ggcaaatgtt cgcgatgccg cccttcggtt tccagaccgc cgtccgcgcc gatgcgccgc
     8521 gtgttgccga tgcgatggaa tttctcaatg ccgccaaaga aaccctcgcc ccgctgttgc
     8581 ccgaaagcgt ttcccagttt ggcgccgccc ctatgctgat ggtgcgcctt gctgaacgcg
     8641 aacgcgcgca aatcttcctc gaatcggtat cccgacagga tttgcaccgc gccgtgagtt
     8701 tgtgggtgca ggtgttgcag caaaaccgcg acagcaaaat cagatggtcg gtggatgtcg
     8761 atccgcagga ggcttgatta tcggcaatac gatgccgtct gaaaccgttt cagacggcat
     8821 ttttattccg ggtcgcccgt aaacgcattc gcccgaaaaa tcggaacaaa cgtgaaaaga
     8881 taaagcgtga acacgacggc ggtcagaatc gcggggacgg tgatgaagaa tatcgggttc
     8941 acgttcatta aaacagcgcg cgaaacggcg gcggcgaaaa gaatggggat ggcgatgcgg
     9001 cagagtttgg ggtagtcaag tttggtaaag ccgctgtgcc acagtccggc agtcagccac
     9061 accatcatca cgccgcccat catgccgccg agagtaatca ggtgcagggg cgcggaggag
     9121 ggcagatttt gcagtttcgc cgcgcctgtc cacagatagc ctgcggcggc aaagagttgg
     9181 agtaggtaat aggtgcggac gtagtgtttg cgcaggagtt cgtgatggtg cagttcgcgc
     9241 agcttggcga gcaggatgaa accgacggcg aatgcggtaa aaccggcggt ttgcgcgggc
     9301 agccaaagtt cggcggcggc gtgcagcagc aggaaggtga tggcgatgtt tttatagacg
     9361 acgttgggga tgaatacggg gtctttcaga cggcattctt tcaacgcttc cgcacccagc
     9421 aaaacactca cgcgtatgga aacaaacatc accgccgcca tattcaaatg cacttgcgcg
     9481 cgcagtaagt tcaaatcgcc gctgacggca taggccgtct gaaaaacggt aaatgcggca
     9541 agcaacgtca aaagagcgaa gttgtcggtg ttgcggtcga gccaaatcag ccgggcgcag
     9601 aacagcagca ataccagcca ataggcggca acgaaaaacg aggcagtttg cggcgaaaag
     9661 ggcagtatag cggatgcggc gagcaataat gccgccatca aagtcgcgac aggtttcagg
     9721 ttacccgaaa aaaaccgtcc agtccaacaa agccgcagtc aggaaaccac catatgccgc
     9781 aggtagcatg agttctaaga agatttggcg gtgcaggacg acggcaccgg ggctgatgaa
     9841 aaacaccaac gcgccgagta tggcaagcac tgtcgcgccg acgaaaaacg ggcgcatagc
     9901 aactgtattt ttcaccccgt cgggcaaaaa taccaaaact caaatcaagc cgtccggata
     9961 ccgttttcgg cggtatcgtt ttcggcggta tcgttttcgg caaaataatc acgcatccgg
    10021 gcattcgata tcgtcagcag tttacgcata cataccgtaa cggcaacctt atacggctta
    10081 ccctcggaca gcaggcgttg gtagaaatcc cgaataagcg gttcaaaacg tgtcgctgcc
    10141 acggtagcca tatacagtgc cttaagcacc gc
//
LOCUS       DAWHRA010000074         9869 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWHRA010000074 DAWHRA010000000
VERSION     DAWHRA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.074"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="WIB30_001341"
     CDS             complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="WIB30_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ3797405.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2288..2560)
                     /locus_tag="WIB30_001342"
     CDS             complement(2288..2560)
                     /locus_tag="WIB30_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797406.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVALSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2746..3432
                     /locus_tag="WIB30_001343"
     CDS             2746..3432
                     /locus_tag="WIB30_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3797407.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3564..3902
                     /gene="erpA"
                     /locus_tag="WIB30_001344"
     CDS             3564..3902
                     /gene="erpA"
                     /locus_tag="WIB30_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ3797408.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            4062..4526
                     /locus_tag="WIB30_001345"
     CDS             4062..4526
                     /locus_tag="WIB30_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ3797409.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPTRNIKPLVFGLLIAFLGYLLGKIF"
     gene            4561..6072
                     /gene="ubiB"
                     /locus_tag="WIB30_001346"
     CDS             4561..6072
                     /gene="ubiB"
                     /locus_tag="WIB30_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ3797410.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(6276..7094)
                     /gene="cysE"
                     /locus_tag="WIB30_001347"
     CDS             complement(6276..7094)
                     /gene="cysE"
                     /locus_tag="WIB30_001347"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ3797411.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            7278..7856
                     /gene="grpE"
                     /locus_tag="WIB30_001348"
     CDS             7278..7856
                     /gene="grpE"
                     /locus_tag="WIB30_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ3797412.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(7980..8195)
                     /gene="nqrM"
                     /locus_tag="WIB30_001349"
     CDS             complement(7980..8195)
                     /gene="nqrM"
                     /locus_tag="WIB30_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ3797413.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(8221..9276)
                     /locus_tag="WIB30_001350"
     CDS             complement(8221..9276)
                     /locus_tag="WIB30_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ3797414.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPNSEISRFNQTTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDSASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(9570..>9869)
                     /locus_tag="WIB30_001351"
     CDS             complement(9570..>9869)
                     /locus_tag="WIB30_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3797415.1"
                     /translation="KGYDSEENRQHLEEHQLLDGIMRKAHRKHPLSEVQTKRNRYLSK
                     TRYVVEQSFGTMHRKFRYARAAYFGLSKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT         2206 a         2233 c         2724 g         2706 t
ORIGIN      
        1 aaaaatttcc agagaaccga tacaacagtt ggaacttggg tttgggaata ttacggtaga
       61 tgaacttgga acctctgtta tgctatggtc ttttatctca attgaaaaaa gcgcgaatca
      121 aacggttcgc gcttttttca gacggtatta attatttttt gtcgtctttt acttcttcaa
      181 agtcggcatc tacgacatca tcgtctttct ttgcagaagc attggcttgt tcgctttcgc
      241 ctgcttgggc ttcagcttgt gcttgagcgt aaaccatttc ccccagtttt tggctggctg
      301 cgcccagcgc ctcggttttg gcatcgatag cggctttgtc gtcgccttta actgcttctt
      361 cggcttcttt cagcgcggct tcgatttttt ctttctcggc tgcgtcgagt ttgtcgccgt
      421 agtcggccaa agattttttc acagagtgaa tcagggcttc agcttggttg cgggaagcga
      481 ccaattcagt cagtttttta tcttcctcgg cattggcttc ggcatctttc accatgcgtt
      541 cgatttcttc ttcgctcaaa cctgaagaac cttggatggt gatgttggct gctttgccag
      601 tgcctttgtc tttggcggaa acgtgcagga taccgttggc gtcaatatcg aaggtgactt
      661 cgatttgcgg cataccgcgc ggtgcgggtg cgatgtcgcc caagttgaac tgacccaaag
      721 atttgttggc agaagcgcgt tcgcgttcgc cttgcagtac gtggatggtt actgcgcttt
      781 ggttgtcttc ggcggtagag aacacttgcg acgctttagt cgggatagtt gtgttctttt
      841 gaatcagctt ggtcatcacg ccgcccatgg tttcgatacc caaggacaga ggggttacgt
      901 ccagcagcaa tacgtcgctg cggccgccgc ttaacacttc gccttgaatc gctgcgccta
      961 cggcgacagc ttcgtcaggg ttcacgtctt tgcgcggctc tttgccgaag aagtctttaa
     1021 cggcttcttg tactttcggc atacggcttt gaccaccgac caaaatcacg tcgtcgatgt
     1081 cgccagtgct cagaccggca tctttcaatg cggtgcggca aggctcgata gagcgggcaa
     1141 tcaggtcttc aaccaggctt tcgaatttgg cgcgggtaat tttcatcgcc aagtgtttcg
     1201 ggccggttgc gtccatggtg atgtacggca ggttgatttc ggtttgctgg ccgctggaca
     1261 attcgatttt ggctttttcg gcagcttctt tcagacgttg cagagccatg acgtcttgtt
     1321 tcaaatcaat gccttgttct tttttaaact cgtcaatgat gtagtcgatc aagcgttggt
     1381 cgaagtcttc accgcccaag aaagtatcgc cgttggtagc caatacttca aattgtttgt
     1441 cgccgtcgag gttggcgatt tcgatgatgg aaatatcgaa agtaccgccg cccaagtcat
     1501 atacggctac tttgcggtct ttgttgtcgc ctttgtccat accgaatgcc aaagcggctg
     1561 cggtcggctc gttgatgatg cgttttacgt ccaaaccggc gatacggcct gcgtctttgg
     1621 tggcttgacg ttggctgtcg ttgaagtagg ccgggacggt aatcacggct tcggttactt
     1681 tttcgcccaa gtaagcttcg gcggcttctt tcattttacg caggacttct gcggaaattt
     1741 gaggaggaga cagctctttg ccttgtgctt ttacccatgc gtcgccgttg ttggctttga
     1801 tgatttcgaa aggcatagat tcgatgtcgc gttggacttc tttgtcttca aatttgtggc
     1861 cgatcaaacg tttggcggcg taaatagtgt ttttggcgtt ggtcaccgct tggcgtttgg
     1921 caggcgcgcc gacgaggatt tcgccgccgt ccaaataagc gataacggac ggcgtggtgc
     1981 gtgcgccttc tgcgttttcg ataactttgg tttgaccgtt ttcggaaatg gccaaacaag
     2041 agttggttgt acctaagtcg ataccgatta cttttgccat gtggataatc ctatttgatt
     2101 ttgcttattt tgagaaatat gttggaacat tttgtcccga tgggctgtaa atagggcggg
     2161 cggcgggctg tttcaagcta cagcatggct ataagtatat aactttatga atatattggt
     2221 tttatatttg atttaataca tttggctcca atgcattcaa gcataatgtt tcaaatggca
     2281 ggcaggttta ttcatagacg atgccggcga gcatttcctg ttcgttcaag ttgccgtact
     2341 ctttttccca gtcgtgtgaa gactcgatga tgtcgcattc tttggaaagg gcgacttgtt
     2401 ctgcatccat atctttggcg ttcagtatgt tgaattgttc gcacagggat gcggataaag
     2461 tgatgtcagg ctgtttggct tcagaacggt tttcttggaa ggcaaagcag aatgcggtaa
     2521 atgccgcagc atagataaga tatttgccgg ttttcttcat tttctatcct ttttctgtca
     2581 attcaggatt aaacctatgg aaaatcttaa aaattatgta ttgagtaaga aaaatcataa
     2641 tttaaattta gtttatcata gttgttccgt ttttgggata gctaaggtaa aatatatttc
     2701 atgtttactt tagatgattg aatgaagggg agcggaagga tatttatgga aacctttaaa
     2761 gacagactgg tttttttatg gaaaagcgaa gcgaagcagg caaaaatcgc atccgatatt
     2821 gaaatgacga ttgcgggctt cagcaggata tggaatgaag gcggtttgcc gaagtctgaa
     2881 acattaaaaa aaatcaagca gttgaagggg tgtagtatcg attggctgct gaccggggag
     2941 ggtaatccgt ttccggatga agccccaaaa aaatcccttg cttacgatac tttgggcaat
     3001 gaagtcgata cggacgagtt tgtcttcgtg ccgagatatg atattcgggc ggctgcggga
     3061 tacgggcagt ttgtcgatca tgaggaaccg gtatttacaa tggcgttcag acggcattgg
     3121 attgagaatt atgttacccg cgatacgaaa aacctgtctg taatttccgt caagggggat
     3181 tcgatggagg gggttttgaa tgacggcgat tcgattttgg tcaatcatgg tgaaaatacg
     3241 ccgagggacg gtctgtatgt gttgcggatt aatgaaaatc tgctggttaa acgtttacag
     3301 attgtaccgg gcgggattat caatgtgatt tctgcaaacg aggcttatcc tgcttttgaa
     3361 atcaatttga acgatttgac cgatgatgtg gagattatcg ggcgtgtcga gtggttcggc
     3421 aggacgattt gagtttgggg cttgaaattg caggcggtca aacttatcta ttggaacaat
     3481 tcctttttca aaggcgaagc ctgcttgcct ttgaaggggg tttgagagag aatgcagaaa
     3541 atattatatt aaggaataac accatgtcgg atgaaagccc tattattttt actgacagct
     3601 gctgtgccaa agttgccgat ttgattgccg aagaaaacaa tcccgatttg aaattgcggg
     3661 tttttgtcaa tggcggcggc tgttcgggtt tccagtacgg atttactttt gacgaaatca
     3721 aaaacgacga cgattttgaa attgagaaaa acggtttggt ctttttggtc gatccgatga
     3781 gctatcaata tctggtcggt gcggaaatcg actatacgga aagtttgcag ggttcgcaat
     3841 tcgtcatccg caatccgaat gcggaaacaa cctgcggttg cggatcgtcg ttttccgtat
     3901 gaccgcttgg tttgtgtgat gccgtctgaa cgttcagacg gcatttttac ttttagaaaa
     3961 tatattatcg ggatgaattc acatataatc cgattgtttg aagatgaatc gggtttcccg
     4021 aaaggaacgg gcggaacggt atcaggcgta tttgttccct tatgattgag atgagtaaag
     4081 attaccgaaa cgatttgtac gatgtatatg tttcttaccc gccccaagtg gatcgcgggc
     4141 ttatccggga gtgccttaag gagaatctcg gcgaggaaaa ggcggaagga ttgatcgaat
     4201 cgctcgattc caaacctcaa gtgctggttg aggaaaaatg cacttgggcg aaacgggaag
     4261 agttgcatga ttatttcagc tatttgggtt tggatattat tacccggaga tatatggagt
     4321 tggaaacggt cgtgccgccg gaggaaggtg agggcgaagg agagggcgcg gatggggaaa
     4381 tgcccgaata tcttgaactt catggcgggc gggaagatga tatttccgca ccttcgcaac
     4441 ccgaaccgcc gacccgcaat atcaaaccgc tggttttcgg gctgctgatt gcctttttgg
     4501 gctatctgct cggtaagatt ttttgattgt ccgataaatg ctgtattcgg gattttatat
     4561 atgaaatggt tgaaacgcct gacggtcatt gtcgggactt tttaccgcta tcggctggca
     4621 ggtctgtgtg cttcgctgat gggtagcggt tggatatgcg ctctgctgaa aatgatgccg
     4681 cagtcgtcca aattgaaaaa cgaaccgcct gctgtccgtc tgcgccttgc cttggaaagc
     4741 ctggggccga ttttcatcaa gttcgggcag gtattatcta cgcgccccga tttgattccg
     4801 cacgattacg ccgtcgaact ggcaaagctg caagacaaag tgccgccttt tgacgcgcgg
     4861 ctttcgcgtg aacaaatcga aaaatcgttg ggtcagtcca tcgaaaagct gtatgcggaa
     4921 tttgaaaccg agcccatcgc cagcgcgtcc atcgcccaag tacacaaagc ccgcctgcat
     4981 tcgggcgaac gagtggcggt taaagttttg cgccccaacc ttttgcccct tatcgaacag
     5041 gatttgtcgc tgatgcgttt cggcgcggcc tgggtcgaac gcttgttttc agacggcagg
     5101 cgtctgaagc cgctcgaagt ggtggcggag ttcgacaaat acctgcacga cgagttggat
     5161 ttgatgcgcg aagccgccaa tgccggacag ctcgggcgca atttccaaaa cagcaatatg
     5221 ctgattgtgc ccaaggtgtt ttacgactac tgcaccagcg acgtgctgac aatcgaatgg
     5281 atggacggca cgccggttgc cgacatcgcc aaactcaaag cagacggcat cgatttgcac
     5341 aaactcgccg attacggcgt ggaaatcttt ttcacgcagg tcttccgcga cggctttttc
     5401 cacgcggata tgcaccccgg caatattttg gttgccgccg acaaccgcta catcgccctc
     5461 gatttcggca tcgtcggcac gctgaccgat tacgacaaac gttatctcgc catcaatttc
     5521 ctcgcctttt tcaaccgcga ttaccggcgc gtcgccaccg cccacatcga atcgggctgg
     5581 gtgcctgccg acacgcgtgc ggaagaattg gaagccgccg tccgcgccgt gtgcgaaccg
     5641 gtgttcaaca aaccgatttc gcagatttcc ttcggcttgg tgctgatgcg cctgtttgaa
     5701 gtcagccgcc gcttcaatgt cgaaatccaa ccgcagcttg tgctgctgca aaaaacgctg
     5761 ctcaacatcg aaggcttggg ccgccagctc gatcccgatt tggacttgtg gaaaaccgcc
     5821 aaaccgtttt tggtgaaatg gatgaacggg caggttggcc ctaaagccct ttggcgcaac
     5881 ctcaaaaacg aagcccccga ctgggcgcaa atcattcctt cattgccgcg caaaatcaat
     5941 gccttggtcg atgaggcgcg ccggcaggaa atgcgcgatg cctatattca tttggttaaa
     6001 gtgcagcagc ggcaaagcct gtggctggct gtgattgcgg ttgttttgct gctgattttg
     6061 cttttgaaat aggctttgtc cgaattatcg cccgactccg cccgtttaca aggaaaccgg
     6121 ttatttccgg ttgcgtggga atcgggtgta tcgaataaaa ggcattttgt ccgactggca
     6181 agtgccgaca tcggcggcat atcaaggcgc aggcttgaag cgggcaatgt cgtctgaagc
     6241 ccgtttggcg tttcagacgg cattggtgcg gatattcaaa tcataaagtc gatttcggta
     6301 aactggatat tttgatccat atccgccgac ggtgttttga gcgatcgcgc cacgggtttg
     6361 gcgggtacgc cgacaaccgt gatggacggc ggcacgtctg aaaccacgac gctgcccgcc
     6421 ccgattttgg cattgctgcc gatgcggata ttgcccaata tcgaggcgtt tgcgccgatc
     6481 atcacgccgt cgccgatttt agggtggcgg tcgccgcctt ctttgcccga accgccgagc
     6541 gttacgccgt gcaaaatcga aatattgttg cccaacacgg cggtttcgcc ggcaacaaag
     6601 ccggtggcgt ggtcgagcat cagcccgtat ccgaaacggg cggcgggatg gatgtccacg
     6661 ccgaatactt cggacatacg gttttgcagg aaatacgcca gcgttttgcg cccgtcgaga
     6721 tacagccggt ggttgatgcg gtgtgcctga atcgcgtgga agcctttgaa atataaaagc
     6781 ggcagcgaat attcgtcgca ggcgggatcg cgttcgtaga tggcttttaa gtctgcttcg
     6841 acgcatttgc cgatttgggt gtcgctgccc aacgcctgct ggtagatttc aaacagcgcg
     6901 cgcacgtcca taatcgggct gccgagtttg ctggaaaggt ggtaggcaag gacggagccg
     6961 agggactcgt ggcgcaacac ggtttggtgc aaaaaacttg ccagcatcgg ttcggcggag
     7021 accgcggccg cggtttcttc gcggatggtg tgccagaggt cgaaaccggt tgtgtttaaa
     7081 tggtcttttt tcatgagtga tgacgtttga aaatcgatat ggtcggcagt atcttaccgt
     7141 ctatattatt ttttcggtag gggatttgaa aatgaatttg aaattctctg cttttgcttg
     7201 aagtttcttg aaaatgtcct tatcttgcgc gggtaataac tggattttga tttccaattt
     7261 gttttaaggg atacgatatg agcgaacaga cacagcagca aaacagtgaa gaagcggttg
     7321 aaaatgtgga ggcggtggaa accgtcgaga cagtaggaaa tgcggacggt gtgcaggaac
     7381 aggctgccgc agagccggct tatgaggatt tgcaggcgcg gattgccgag ctggaagcgc
     7441 agttgaaaga cgagcagctg cgcgctttgg caaacgagca aaacctgcgc cgccgccacc
     7501 agcaggaaat tgcggatacg cacaagttcg ccggacagaa gtttgccgtg gaaatgctgc
     7561 cggtcaagga ttacctggaa atggcgcttt tggatcagag cggcaatttc gatgcgctga
     7621 aaatgggcgt gcagatgact ttgaacgagt tgcagaaagc atttgatgct acgcaaatca
     7681 aggaaatcaa tcctaaagcg ggcgataagc tcgatccgaa tatccatcag gcgatgcagg
     7741 cggtggcaag cgaacaggaa cccaataccg tggtgggcgt gatgaagaag ggttatacgc
     7801 tgtccgaccg cgtgttgcgc ccggctatgg ttacggtggc acagaaggaa gcctgaaggc
     7861 gtctggggaa taatctgatt tatttcctga agcgcgtttt gcgtataaac cgatcgaagt
     7921 aaagcggcaa tgccgtctga acccgcctgt cgggcttcag acggcatttt gtttcggcgt
     7981 taacggtcaa cccgtatgcc gcctgcctgt ttttcttcat ccagtttctt ttgcagggtg
     8041 tcgcaaggtg tgtcgcagtc gcacattttt ttcataccca aggcagtaat gccgccgcaa
     8101 ctgcctttga tgctgcgttt ggagaaaata tagccgaccg ccataccgat gatgacggtc
     8161 aggaagatgc cgaaggtaag gagcagggtt ttcatggtgt ttcctaatcg gtttgtatgt
     8221 ttagcggagc agtttttcaa attcggaaga catcgcggtg cggtagccgc ctttatccct
     8281 gacaatcagg aaaacagcga gtttttcgcg ctctgccagc tttaaggctt cggtttcgcc
     8341 caatacgaat aatcctgtgg acaagccgtc cgccgtcatc gcactgtctg cgaccacgct
     8401 gatggaggcg aggttatggc tgatgggtcg tttgttgttc ggattaatga tatgggagag
     8461 gcgtttgccg cttttatcga cgtggaaaat acggtaatcg ccggaagtgg caagcgaacg
     8521 gttgttcagc gggacgataa tctgcgtatt gccgccttgg atgatattgg gttgctcgat
     8581 accgatgcgc cacggttcgc cgcgcgcgtt tttgcctttg ccgtgcaact cgccgccgat
     8641 ttcgaccaga taattttgaa tgccgtattt ttccagttcg cccgcaactt tatcaacgcc
     8701 gaagcctttg gcaatcgaag ataaatccaa ataggctttg gggtgggttt tgctcaagga
     8761 ggcggaatct ttgccttgtt tcaaaatgat tttgtctatg cccgtataag atgccgcctg
     8821 tttgatttgt tccggcgacg gttcacgggt aacggatttg tcgggcccga atccccaaag
     8881 gttgaccaaa gggccgacgg ttacgtccag cgcgccgtgt gtcaggcggt tcaggcggac
     8941 agcttcggca gtaacgtgtg cgaagtcgct tgaaatgcgg aggggtttgc cggccgtggt
     9001 ttggttgaac cggctgattt cggaattagg ctgataggtg gacatctgcc ggttgacttc
     9061 tttaagcgcg tcatcgatgc gcttttgtat ttcggcaggt gaggggagtt tgtcccgatt
     9121 atttgaaagg tatttgacgg tataggtcgt gcccatcgtt tcgccttgca gggtaacggt
     9181 ttgcgcggtt tgttccgaac aggcgttcag gaagatgaaa cccagggcaa atatcaagac
     9241 gcggataaag ttcggcaggc gtgtttcaga cggcatagtg tttgacggtt ttggcaaatg
     9301 gtttgaatta tatcgcaaaa cggccgatat gtttctatgc cgatgccgtc tgaagggcat
     9361 caggggcatc gcccgctgtt tgggggcggt aataaaaaaa caaaccggtg ttttacaaac
     9421 cgagaccttt gcaaaattcc tttccctccc gacagccgaa acccaaacac aggttttcgg
     9481 ctgttttcgc cccaaatacc gcctaatttt acccaaatac ccccttaatc ctccccggat
     9541 acccgataat caggcatccg ggctgctttt taggcggcag cgggcgcact tagcctgttg
     9601 gccgctttca aaaggttcaa acacatcgcc ttcagatggc tttgtgcact cactttgctc
     9661 agcccgaaat aggctgcccg cgcatagcgg aatttacggt gcatcgtacc gaagctctgt
     9721 tcgaccacat aacgggtctt cgacaaatat cggttgcgtt tggtttgcac ttccgacagt
     9781 ggatgtttgc ggtgggcttt gcgcataata ccgtctaaca actgatgctc ttccagatgt
     9841 tgccggtttt cctcactgtc gtagccttt
//
LOCUS       DAWHRA010000075         9860 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWHRA010000075 DAWHRA010000000
VERSION     DAWHRA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.075"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(137..832)
                     /locus_tag="WIB30_001352"
     CDS             complement(137..832)
                     /locus_tag="WIB30_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ3797416.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPVE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKLLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(947..3058)
                     /locus_tag="WIB30_001353"
     CDS             complement(947..3058)
                     /locus_tag="WIB30_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ3797417.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGTVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNLTLNGDYNIGRFENHLTVGMDYSREHRNPTLGFSSAFSASINPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSIDTLSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDPKNNPYIYAVSGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAMLGWNHKNVNVTFAAANLFNQKY
                     WRSDSMPGNPRGYTARVNYRF"
     gene            complement(3244..3849)
                     /locus_tag="WIB30_001354"
     CDS             complement(3244..3849)
                     /locus_tag="WIB30_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ3797418.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(3898..4296)
                     /locus_tag="WIB30_001355"
     CDS             complement(3898..4296)
                     /locus_tag="WIB30_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ3797419.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            4404..5315
                     /locus_tag="WIB30_001356"
     CDS             4404..5315
                     /locus_tag="WIB30_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ3797420.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENIV
                     QAKLLHRNSRNLSLTGAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKASACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            5305..6606
                     /locus_tag="WIB30_001357"
     CDS             5305..6606
                     /locus_tag="WIB30_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ3797421.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKHSPADSDIFATYRQPFPENLSAPQPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDAPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGGLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTYPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(6674..6898)
                     /locus_tag="WIB30_001358"
     CDS             complement(6674..6898)
                     /locus_tag="WIB30_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797422.1"
                     /translation="MVFWLQRLKYPLLLCIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLLAMGTVSAWVAVIWAYLIIESEKNGRY"
     gene            6998..9148
                     /gene="dinG"
                     /locus_tag="WIB30_001359"
     CDS             6998..9148
                     /gene="dinG"
                     /locus_tag="WIB30_001359"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ3797423.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGDPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQNNAQQNLTGFETPEVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQMTGKIAALTDKAELANLADETAASLLDSLHEWQFHLAE
                     EPSLSLGVSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDSALLDRLTGEFGLFIARIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLILRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            9184..9798
                     /locus_tag="WIB30_001360"
     CDS             9184..9798
                     /locus_tag="WIB30_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ3797424.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT         2324 a         2870 c         2520 g         2146 t
ORIGIN      
        1 ggtttaaatt taatccacta tatattctga tttaaaacca taaggcttta agcaatcatc
       61 tcttctataa agcctaaata caaaaggccg tctgaaatcc tattttcaga cggcctttac
      121 tcattcaatc ctcaacttat tgcggctttt tctgctcttc gcgtacttta tccaccaact
      181 ggtcaatcgt ggtcataccg gactgccagt ctttaaattc aacttggtat ttgccgccga
      241 cgatcacagt cggtgtgccg ctgatttgga atttattggt caactcttcc atttgagccg
      301 cacgcgcttg gctttcagga gcctcaaatg cagccaataa ttttttgccg tcaaacgctg
      361 tttgctcgga cagccatttt ttcagggtat cggtatcggc cagattgatt ttttgattaa
      421 ccatcgcatc gaaaatatgg ctgttggctt tatctgattc accggccatt tccactgcgg
      481 ccgccaaacg tgccaaaggt ttcatttcat caccccacac gacatgctcc cggcgcatat
      541 aggtatcgtc tttaaacgtt ttgatgtgct cgctcaagac cggctcaaga tgggcgcaat
      601 gcgggcagaa gtagccgaaa aattccaata cttcgatttt accggcctgc tgttgcggaa
      661 taggcgtaga caatacagtg tagttcacac cttcgttcaa ctcaacaggg gctgccggag
      721 cagatgagct gctttggacg ctgtctgccg gaacgctggt ttcagcctgt ttgctacaag
      781 cggccaatgc caacagggtc aatgaagtca aagctaaggt tttcagtttc ataggtatct
      841 cttgtgtgtc agattaatgt gcggatttta tgacatttta ttgaaggcgt gttcgatttg
      901 agtgaaaaaa tgtgtttagt tttatttcag cctttgcctg atttcatcag aaacggtaat
      961 ttacccgggc agtatagccg cgcggattac ccggcataga gtccgaacgc caatattttt
     1021 gattgaacag attggctgcg gcaaaggtaa cgttaacatt tttatggttc cagccaagca
     1081 tggcatcaac tcgggcaaag cctggaagcg tagtcacttc tttatttctt gagttgtaac
     1141 cgtagcgttt gcctgtaccg gttacgccga tttcgccgta gaggttttcg gtcggggtat
     1201 aacggaaaaa caggttgccg gtaacgttgc tggtattatt caaatggatg cccactcggt
     1261 cgggattttc tttgtcttca acgactttcg cctgcatcac gcccaacgaa ccgcgcagat
     1321 agagtttttt ggggatgatt tgcccgatgg cggacaattc cacgccgcgc gaacggtgtt
     1381 tgccgctaac cgcataaata taagggttgt tttttggatc ggggcggtag cggatattga
     1441 agcgttcgat ttggtaggca gacaacgtag tgctgaggcg gtcgtccagc caactgcttt
     1501 tcacgccggt ttcgtattgg cgggtgtact cggggtcggc gttgaacacg gcggaagaca
     1561 acgtatcgat gctcaaatag ccgccgcgtc cgccataagg cgcgaagcct ttgttatacg
     1621 aggcgtaaag tgtgtggacg ggattgatgt tccacactgc gccgatgttg gggctgaacg
     1681 agtgtccgct gtattggcgg ctgctgccgg tgagtttgtt ttcggaatta aaggtgtatt
     1741 tgtcgtaacg gccgccgagg acgaatttca aatcgggcgt ggcggagaag atgttttgca
     1801 caaagatgcc gtaggagtcg gctttgtggc ggttttgggt cagaataggc tgcaatctgc
     1861 ccgaagccgg ccagcttgcg cggtcgtagg ggttgatgga ggcggaaaag gcgctgctga
     1921 aacccaatgt cgggttgcgg tgttcgcggc tgtaatccat gcctacggtc aggtggtttt
     1981 caaaacggcc gatgttgtag tcgccgttga gcgttaagtt ggacgacagg gttttgttgt
     2041 cggtctgctg ccaggcgtag ttacgtttga ttaagttgcc attttcgctg cctgcataga
     2101 aatgatcaaa atcctgcgcc gccgtgcggt gggcgagctg ccattgggca cgccatttgt
     2161 cgttgaaggc gtattcaagg tcggaacgcc aaacttgcag cttgtctttg acaaaatcgt
     2221 tccggtgggc gaaccccatg cggtaaggca gtccgaagcg gtcgtacacg gacttggtcg
     2281 gactgcggtc gggcgtgcgc tccacattgt cgtaggtgta ttgccccgtc cacttcaagc
     2341 cgttgtcgag tttgacggta atgctgggcg aaaccatgac atttttgctg tctatgccgc
     2401 tgcggaacga attggcgcgc ccgacttcgc cggtgaggcg gatggcgacg tttttgttca
     2461 gcacttcgtt gatgtccata ttcaggctgc ggtttgccca cgaaccataa accgtaccga
     2521 tattacggct ttgtttgaag ttggcgtatt tgctgaccat gttgatgaca ccgccgccgt
     2581 tggtacgccc ataaagcacg gaggacgggc ctttcaggat ttccacgcgc tcgatgttgg
     2641 cagtactgcg gcgcacttgt ccgctttcgc gcacgccgtc gcggtaaata tcggatgcgt
     2701 cggcttgaaa accgcgcagg aaaatgcttt caccgcgcat atcgtaggca gcgtcgatgc
     2761 cggcattgcc ttcgaggatg gaactcaaat cgttcgtacc gtaatttttg tttttctgga
     2821 tattgagcgt atcgatggtt tgcggcgttt ctttgatgag ctgtccgttg cgggtaacgg
     2881 cggcttcgtc gtagttgatg tagcctttga gtacgctggt gtcggactgt ccgaccacgg
     2941 aaacggtagg tagcgtggcg gtgtaatgtt caccattgtc ctgcgtatcg gcggcagcaa
     3001 cagggaagga agcaataatc agcgtgggta ataaagctaa atgaaatgat attttcattt
     3061 ttatactcaa tttaacaaaa caaccgaatt atattgcctc acggaggaaa tgagaataat
     3121 ttcttttaac tatattgaac atgatatttg taaacaaagg tctcagaatg aggaaaactc
     3181 gccgcctgat actgaaaaat gccgtctgaa cagggttcag acggcatttt tttgaccgcg
     3241 aaattatgcg ccgaacactt tcaaacgttc tgcaactggt tcaaaggtct tttcacctgc
     3301 cgccccttca ataccgccga taaccatttg tgcgcgcaac aaccagtttt cggggatatt
     3361 ccacgctttg gcaatcgccg catcgggcaa gggattgtaa tgttgcaggt ttgcacctac
     3421 gccgaccgcg gcaagtgtcg tccaaacggc atactgcacc atcgcgttcg cctggtccgc
     3481 ccaaacggga aagttggcgg cataagcagg gaactgctcc tgcaaacctt tgacgacatt
     3541 ttgatcttca taaaacaaaa tagttgccgc acccgcctta aacaggttca atttttgcgc
     3601 ggtcggttca aaactgtcgg caggcacgac ggcacgcagc gcgtcttcga caaattgcca
     3661 caccttatca tgctcttcgc caaacagcac gaccacgcgg gcagattggg aattgaacga
     3721 agaaggtgtg tgcaaaacgg cgtgttcgac gatttggaca acttcatctt tgccgacggg
     3781 cagattttta tttaacgaat aaatggaacg gcggctttcg gcagcctgtt gcagagattg
     3841 acgggtcatt tcttttcctc aaaaacattc aacaaaatac actttaggca gtccgattca
     3901 ggctttacgc ttgaaaaaca gcctgtccag cgaccatgct ccgccgcccg ccgccgcgat
     3961 atagaggaat acgaagcaga acagcactgc ggactcgccg ccgttggcaa tcgggaacaa
     4021 agcatttccg gaagcgtgcg ccataaaata ggcaaccgcc atctgaccgg acaaaacaaa
     4081 cgcggcaggg cgcgcaaaca ggcccaacac cagcaaaatg ccgccgacaa tttctaaaat
     4141 accggcaagc agcaacagcc cgccgggcga accgctgccc atttcaatgg ggaaggcgaa
     4201 gattttcgac gtaccgtgca acaaaaacag gtaggcggtt acgatacgca aaacagaaag
     4261 caaaaccggt tggatacggt tgcagcaatc ggacataaaa gctcctttga tatttgtctt
     4321 catttcaggc ggtagagtat agatgtttcc ccttttcaaa tatatactgc ttttattgat
     4381 acaacttttc cacacaagaa aatatggaca ccctgttcag cctcaaggtt ttccgccaag
     4441 tcgtccaaag cggtggcttc acccgcgccg ccgacgcgct cggcatctcc accgcaatgg
     4501 caagcaaaca cgtcagccac ttggaaaaca tcgtccaagc caaactcctg caccgcaaca
     4561 gccgcaacct cagcctgacc ggagccgggg aagaatacta ccggcaatgc agttacgcgc
     4621 tcgacacgct cgacgatgcc gcgcaaaaag ccgccggggg gacggaaaaa ccgcaggggc
     4681 tgctgcgcgt aacgatgccg ctgtggtttg ccggcagcca gatatgcaac tggctggcgg
     4741 aataccgcga acgttatccc gaagtaacat tagaactgat tttggacaac cgccacgtcg
     4801 atttgattgc cgaaggcgtg gatttggcgt tgcgcgtttc ccaaaccctg tccccgtcgc
     4861 tgattgcgcg cccgctggcg gaaatcgaat ttgccctgct cgcctcgccc gatttcctga
     4921 ggcgcaacgg cgtgccggaa acgccggaag aagtggcagg gctgcccgcc gtcctgccga
     4981 cctacaccaa ccagcagaaa ctcgacctca cccgcaaatc ggacggcaaa aaataccggc
     5041 ttgaactgac ccccgtcatc cgtaccgaca acacgctgat gatgcgcgaa atgattaagg
     5101 cgagcgcgtg catcggttat cagccgcttt gggcggcgga acacgatttg cgctgcggca
     5161 cgctggtgag gctgctgccc ggatacgccg tcccgaccga ccggctgaac gccgtttatg
     5221 cggacagggc attcttaagc gcgaaagtcc gtagcttcat cgattttctg aacgaaaaaa
     5281 tcgccagcag gaaaggctgc cgaaatgccg tctgaaaccg cccgccccct ttatgcggac
     5341 acgtgcgccg cacacacgct cgtctggttc cgccaaaacc tccgcatccg cgacaacgcc
     5401 gccttatgcg ccgccgttgc cgaaggtttg cccgttatcg gtatttggat tgacgatgcc
     5461 gaaacagaca accctcgccg cgccgcgttc taccgccaat ccgccgccga actcgcccaa
     5521 gggcttgcag ggcgcggcat cccgctctac acggcggcat cgcctgccga gctcgtccgg
     5581 ctcgccgtcc gcctcaatat ccgcgccgtc atcgccgacg aatcccatac ttttgccgac
     5641 aaactcgccg acaacgccct ttggcatgaa ttggacaaac acggcatcgc gttaaccttc
     5701 gtcaatgacc gcgccgtttt cggcaaaacc gacctgatac ccgacggcgg cacggcacac
     5761 accgatttcg accgctaccg cgaagtatgg ctcgaccgct tttccaagca ctcccccgcc
     5821 gattcggaca tattcgcgac ataccgccaa ccgttccccg aaaacctttc cgccccgcag
     5881 cctgccgcgc tttcagacgg catcttcctg ccgcaaaacg gcggcgaaac ggcggcttgg
     5941 cggcagtggc ggcggtttct cgaacaggcg gattcctact ccgttttaaa ggatttcccc
     6001 tcgcgcaaac acacttcgct gatgggcgcg tatttgagtg ccggctgcat ctcgccgcgc
     6061 ctgctcgcgc gggaaagcct cgaacgccgt ctgaacgcgt gggcggacaa catcatccgc
     6121 cgcgattttt tccttcaact tgccttgcag cacgcggatg atgccccttc agacggcaat
     6181 ccggaacaca ccctgcgcct gacgctttgg cagcagggcc agaccggcat tccgattatc
     6241 gatgccgcga tgcgctgttt gcacaaaacc ggtggcctcc accccgccct gagacgcttg
     6301 agcgcggact ttttctgcca cgttttaaac ctcccccgcc gcgaaggcga gatatggttt
     6361 gcccgacagc tgaccgattt cgatgcagca atcaaccaag gcaactggcg gcttgccgcc
     6421 tcacggcaca cctaccccga cattgccgcc gcctcatacc gtaccgaccc ggacggtact
     6481 tttatcaaac ggcacattcc cgaactcgcc cacctgtccg ccgacaccgt ccacacgcct
     6541 tggcggtttg cctgttcggt cgatacccac ggctatcccg cctatcctgt cgccggtatt
     6601 ccctaagcgg tacggcaacc taaaacaaat gccgtctgaa cttccgtttc agacggcatt
     6661 gcgtccggat gactcaatat cttccgtttt tttcactttc aataatcaga tatgcccaaa
     6721 tcaccgccac ccaagccgac accgttccca tcgccagcaa aaaatgctgc caatccgtca
     6781 tcggcggtac aggacaacgg ccgcattcga tttccgcgcc gcccaacaac cggtacagca
     6841 gcatatccgc aatacaaagc aacaacgggt atttcaaacg ctgcaaccaa aaaaccaacc
     6901 acgtccgcac gatttacctc ccccattccg acaacgatat tgtaccgctt ccgtgcaatg
     6961 ttaaaataat cccacttcta ccgccgtaca aaacgctatg ctcaccgatt tagaaaaaaa
     7021 cgccatccgc gaccattacc aaaatatcgg caaaaatctg cccggtttcc gtccgcgcgc
     7081 ttcgcagcgg gaaatgattg cggcggttgc caacgctttt tcgcggacgt tgacgcgtga
     7141 agaaggcggc gatccaccca agcgcgaggg cgagagcatt gccgtaatcg aaggcccgac
     7201 cggcgtgggc aaatcgcttg cctacctgct ggcgggcggc atcatggcgc aaacacgcgg
     7261 caagcggctg attgtgagca gcgcgacggt tgccttgcag gagcagttgg tagaccgcga
     7321 cctgccgttt ttggtcgaaa aaagcggttt ggaactgagc ttcgcgcttg ccaaagggcg
     7381 cggccgttat ctctgcccct acaaactcta tcaactgacg caaaacaacg cccagcaaaa
     7441 cctgacgggt tttgaaacgc cggaagtgtt gtgggacagc aaacccaagc ccgaagaatt
     7501 gaagctgctg cgcgacatcg ccgacgaatt ttccgcccga cggttcaacg gcgaccgcga
     7561 cacttggccg gaaaaaatcg atgacgcgat ttggctcaaa gtgaccaacg accgccacgg
     7621 ctgcctgaaa accgcctgtc ccaaccgtcc ggaatgccct ttttacctgg cacgcgatgt
     7681 cttggaaacc gtcgatgtcg tcgttgccaa ccacgatctt ctgcttgccg acatcagcat
     7741 gggcggcggt gtaattctgc ccgcgcccga aaacagtttc tattgtatcg acgaagcgca
     7801 ccacctgccc aaaaaagccc tcagccgttt tgccgccgaa cattcatgga atattgccgt
     7861 ttggacgctg gaaaaactgc cgcagatgac cggcaaaatt gccgcgctga ccgataaagc
     7921 cgaacttgcc aaccttgccg acgaaaccgc cgcatcattg ctcgacagcc tgcacgaatg
     7981 gcagttccat ttggcggaag agccgtcttt aagtttgggg gtgtctgaaa acgacagacg
     8041 aaccaacagc gaaccgactt ggctgtggga agacggcaaa atccccgaag gcctcgaaac
     8101 caccgtttcc aacaccgcca ttgccgcacg cagcctgctc aaacacgtta tcgggctgaa
     8161 cgatgcgctt tccgctgcgc gccgcgaaaa agaacaagac agcgcgctcc tcgaccgcct
     8221 gaccggcgag ttcggtcttt ttatcgcccg tatcgaacaa atcagcgcgg tttgggattt
     8281 gctctccacc gtccctctcg aaggtgaaga accgctggca aaatggataa cccgccgcgc
     8341 cgacgacaaa aacgactaca ttttcaacgc cagccccatc agcagcgcgt cccacctcgc
     8401 caacagtctg tggcggcgcg cggcaggcgc ggtgttgacc tccgccaccc tgcaatcctt
     8461 gggcaacttt aacctgatat tgcgccaaac cgggctgcaa tggctgcccg aaaccaccac
     8521 cctcgccctc aaaagcccct ttgactttga aaaacagggc gaactctaca tccctcccat
     8581 atacgccagc cccaaagacc ccgaagccca caccgccgcc atcatcgaat ggctgcccaa
     8641 gcttatttcg cccaccgaag ccatcggcac gctcgtcttg ttttcctcgc gcaaacaaat
     8701 gcaggatgtc gccctgcgcc tgcccggaga ctacctgccg ctcttgctcg tacaaggcga
     8761 attacccaaa gccgtcctcc tgcaaaaaca ccaccgggcc atagaagaag gcaaagccag
     8821 catcatcttc ggactcgaca gctttgccga aggactcgac ctgcccggca ccgcctgcgt
     8881 gcaagtcatc atcgccaaac ttcccttcgc catgcccgac aaccccatcg aaaaaaccca
     8941 aaaccgctgg atagaacagc gcggcggcaa ccctttcatc gaaatcaccg tccccgaagc
     9001 cggcatcaaa ctgatccagg ccgtcggccg cctcatccgc accgaacaag actacggccg
     9061 cgtaaccatc ctcgacaacc gcatcaaaac gcagcggtac ggccaacaat tattggccgg
     9121 cctgccaccg tttaaaagga tagggtaaac ataccgccct tcccgcaaca gaaaggaaaa
     9181 atcatgaact tcacccgact gctcaaccaa gtcttaagca cggttcaaaa aaaaggcaac
     9241 acattctccg acagcccgct caattcattc ggcggaggcg cgctggttgc cggtgtcgcc
     9301 tccatgctgc tgaacggtaa aaaccgcaaa accatcacca aaatcggttc gaccgccgct
     9361 ttgggctacc tcgcctaccg gggctatcag atgtggcagc aaaacaaagg gcgggcaacc
     9421 gtaacacaaa gcgatttcca acctgccgga gaaactgaag aaacatacag ccgcaccgta
     9481 ttgcgcacca tgatagccgc cgccgcttca gacggcatga tagacgaagc cgaacgccgg
     9541 actatcgaac aggaaagcgg cacagacccc gaaactgccg catggctcgc cgccgaatac
     9601 cgcctgcccg caagcatcgg ggacatcgcc gccgccgtcg gcaacgatga ggcgttggcg
     9661 gcggaggcct atctggcggc aaggttggtc tgtgccgatt tgtcgcggaa agaaaccgtc
     9721 ttcctcgccc gcctgtcgca ggctttgaaa ctggatgaca atctggtgga gagtttggaa
     9781 aggcaattgg ggttttgatg acacagccgg ggagggaaaa tcatcaaaaa acggttattc
     9841 cgaccccggc ctcaaaacgg
//
LOCUS       DAWHRA010000076         9785 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWHRA010000076 DAWHRA010000000
VERSION     DAWHRA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.076"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..2850
                     /locus_tag="WIB30_001361"
     CDS             <1..2850
                     /locus_tag="WIB30_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ3797425.1"
                     /translation="KLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVWQDLNQ
                     DGISQANELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKMGDLLL
                     AADNLHSRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKE
                     AQLALLDNLIHKWAETDSNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSL
                     SDKAKAAIDAARDRIAVLDAYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAKNIYQN
                     LLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGEL
                     RSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGHN
                     KNVSLYGNDGNDTLIGGAGNDYLEGGSGSDTYVFGKGFGQDTVYNYDYATGRKDIIRF
                     TDGITADMLTFTREGNHLLIKAKDGSGQVTVQSYFQNDGSGAYRIDEIHFDNGKVLDV
                     ATVKKLVQQSTDGSDRLYAYQSGNTLNGGLGDDYLYGADGDDLLNGDAGNDSIYSGNG
                     NDTLDGGEGNDALYGYNGNDVLNGGEGNDHLNGEDGNDTLIGGAGNDYLEGGSGSDTY
                     VFGEGFGQDTVYNYDYATGRKDIIRFTDGITADMLTFTREGNHLLIKAKDDSGQVTVQ
                     SYFQNDGSGAYRIDEIHFDNGKVLDVATVKELVQQSTDGSDRLYAYQSGNTLNGGLGD
                     DYLYGADGNDLLNGDAGNDSIYSGNGNDTLNGGEGNDALYGYNGNDVLNGAEGNDHLN
                     GEDGNDTLIGGAGNDYLEGGSGSDTYVFGKGFGQDTVYNYHVDKNSDTMHFKGFKAAD
                     VHFIRSGSDLVLSASEQDNVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAGNNL
                     VQSMSVFGSNTAATGGNVDANIQSVQQPLLVTPSA"
     gene            complement(3192..5795)
                     /gene="pepN"
                     /locus_tag="WIB30_001362"
     CDS             complement(3192..5795)
                     /gene="pepN"
                     /locus_tag="WIB30_001362"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ3797426.1"
                     /translation="MSKTVHYLKDYQTPAYHILKTDLHFDINEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIAGVPSERFTVEVETEILPVENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSGGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAESRL
                     KNNIFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAMAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDILAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYAEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(5926..6720)
                     /locus_tag="WIB30_001363"
     CDS             complement(5926..6720)
                     /locus_tag="WIB30_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ3797427.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHTGRLKEIRNYCETDAANTYL
                     MYLRFCLVSGRLDADEYEMEIKRMRNYLSAQAGEKPHWEEFVRAWE"
     gene            complement(7467..8336)
                     /locus_tag="WIB30_001364"
     CDS             complement(7467..8336)
                     /locus_tag="WIB30_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ3797428.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEIN
                     LAKCFSEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDIPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPGEDAKADAQRMNRFIEDRVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(8387..8923)
                     /gene="ruvC"
                     /locus_tag="WIB30_001365"
     CDS             complement(8387..8923)
                     /gene="ruvC"
                     /locus_tag="WIB30_001365"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ3797429.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPPDAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQPDAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(8926..9165)
                     /locus_tag="WIB30_001366"
     CDS             complement(8926..9165)
                     /locus_tag="WIB30_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ3797430.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(9196..>9785)
                     /locus_tag="WIB30_001367"
     CDS             complement(9196..>9785)
                     /locus_tag="WIB30_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ3797431.1"
                     /translation="LGWHDDHKNLPVVARIAEDCGIAALAVHGRTRTQMYKGEAAYDL
                     IAETKCRLNIPVWVNGDITSPQKAAAVLKQTAADGIMIGRGAQGRPWFFRDLKHYAEH
                     GVLPPALSLAECSAAILNHVRAMHAFYGETAGVRIARKHIGWYIDEMPDGEQTRREIN
                     RLDSAAAQYDMLAGYLERLAGKTDRWACAYRQNAV"
BASE COUNT         2386 a         2440 c         2499 g         2460 t
ORIGIN      
        1 aaactggcag acggttcttt tgccaaacac ggctacgcgg ctttggccga attggattca
       61 aacggcgaca acatcatcaa cgcggcagac gccgcattcc aaaccctgcg tgtatggcag
      121 gatctcaatc aggacggcat ttcccaagct aatgaattgc gtacccttga agaattgggt
      181 attcaatctt tggatctcgc ctataaagat gtaaataaaa atctcggtaa cggtaacact
      241 ttggctcagc aaggcagcta taccaaaaca gacggtacaa ccgcaaaaat gggggattta
      301 cttttagcag ccgacaatct gcacagccgc ttcaaagaca aagtggaact cactgccgaa
      361 caggcaaaag ccgccaatct tgcgggcatt ggtcgtctgc gcgatttgcg cgaagctgcc
      421 gcattgtccg gcgatttggc caatatgctg aaagcttatt ctgccgccga aactaaagaa
      481 gcacagttgg cattgttaga taatttgatt cacaaatggg cggaaaccga ttcgaactgg
      541 ggcaaaaaat cgccaatgcg actttcaacc gattggacgc aaacggctaa tgaaggtatt
      601 gcactgacac catcccaagt agcacaacta aaaaagaacg ctttagtttc cctttctgat
      661 aaagctaaag cagctattga cgccgcccgc gaccgcattg ccgtgcttga tgcctacacg
      721 gggcaggatt ccagcacact ctattacatg agcgaagaag acgcgcttaa tatcgtcaaa
      781 gtaaccaacg atacatacga ccatctcgcc aaaaacatct accaaaacct gttgttccaa
      841 acccgtttgc agccatattt gaatcaaatc agtttcaaaa tggaaaatga tacgttcact
      901 ttggatttta gtggtcttgt tcaagcattt aaccatgtca aagaaactaa tccgcaaaaa
      961 gcttttgtgg atttggccga gatgcttgca tatggcgaac ttcgttcttg gtatgaaggc
     1021 cgaagactaa tggccgatta tgtggaggag gcaaaaaaag caggtaaatt tgaagattac
     1081 cagaaagtgt tgggtcagga gaccgttgca ttattagcta aaacatcggg tacgcaagca
     1141 gatgatatcc tgcaaaatgt aggctttggt cataataaaa atgtttcttt atatggtaat
     1201 gacggcaacg acactctgat cggcggtgcc ggtaatgatt acttggaggg cggcagcggt
     1261 tcggatactt atgtcttcgg caaaggcttc ggtcaggata cggtctataa ttacgactac
     1321 gctaccggac gcaaagacat catccgcttt accgacggta ttacagccga tatgctgact
     1381 tttacccgag agggcaacca tcttcttatc aaggcaaaag acggcagtgg acaagtgact
     1441 gttcagtcct atttccagaa cgatggctca ggtgcttacc gtatcgacga gattcatttc
     1501 gataacggca aagtactgga tgttgccact gtcaaaaaac tggtacagca atccaccgac
     1561 ggctcggaca gattgtatgc ctaccaatcc ggaaatacct taaatggcgg attgggcgat
     1621 gactatctgt acggtgccga cggggatgac ctgctgaatg gtgatgcagg caacgacagt
     1681 atctacagtg gcaatggcaa tgatacgctc gatggaggag aaggcaacga cgccctgtac
     1741 ggctataatg gtaacgatgt actgaatggt ggcgaaggca atgatcattt gaacggcgaa
     1801 gacggtaacg acactctgat cggcggtgca ggcaatgatt acttggaggg cggcagcggt
     1861 tcggatactt atgtcttcgg cgaaggcttc ggtcaggata cggtctataa ttacgactac
     1921 gctaccggac gcaaagacat catccgcttt accgacggta ttacagccga tatgctgact
     1981 tttacccgag agggcaacca tcttcttatc aaggcaaaag acgacagtgg acaagtgact
     2041 gttcagtcct atttccagaa cgatggctca ggtgcttacc gtatcgacga gattcatttc
     2101 gataacggca aagtactgga tgttgccact gtcaaagaac tggtacagca atccaccgac
     2161 ggttcggaca gattgtatgc ctaccaatcc ggaaatacct taaatggcgg attgggcgat
     2221 gactatctgt acggtgccga cgggaatgac ctgctgaatg gtgatgcagg caacgacagt
     2281 atctacagtg gcaatggcaa tgatacgctc aatggaggag aaggcaacga cgccctgtac
     2341 ggctataatg gtaacgatgt actgaatggt gccgaaggca atgatcattt gaacggcgaa
     2401 gacggtaacg acactctaat cggcggtgcc ggtaatgatt acttggaggg cggcagcggt
     2461 tcggatactt atgtcttcgg caaaggcttc ggtcaggata cggtctataa ttaccatgtg
     2521 gataaaaact ctgacactat gcactttaaa ggatttaaag cagcagatgt tcattttatc
     2581 cgttccggaa gtgatttggt gcttagcgct tctgaacaag acaacgtacg tatttccgga
     2641 ttcttctatg gtgaaaacca tcgtgtagat acatttgtct ttgatgatgc agctatcagt
     2701 aatccagatt ttgccaagta tattaatgct ggcaataatt tggtacagtc tatgtctgtg
     2761 ttcggttcta atactgctgc gacaggagga aatgtggatg ccaatataca atccgtacag
     2821 cagccgttat tggtaacgcc atctgcataa ggagcctaat tacattcatg gcttaaactg
     2881 aaaaacagca atcaagttta ttttgattgc tgtttttctt aatattggga taagggtcgt
     2941 attttaatta accttaatcg gtgcacttct agcaatatag tggattcaca aaaaccagta
     3001 cagcgttgcc tcgccttacc gtactatctg tactgtctgc ggctttgtcg ccttgtcctg
     3061 atttttgtta atccactata attaatatga ctttgcggcc gttttgccat tgcgtaataa
     3121 aacgatgggg aagtgatgat aaaacgtgtg tgtaactata tcagacggca ttgtttttct
     3181 gtttgacggc ctcaatccaa aattttgccg acgatttcgc ccacgtcttt cgacaatcct
     3241 tcctgcgccc gaatgcgctg caatgcttgt ttcaccaagt ttttgcggtg cggctcgagc
     3301 ttgttgcaga ggttgaacgc ctgcaccagg cgggcggcga cctgcgggtt aaagcggtcg
     3361 atttcgatga ctttgtcggc gatgaagcgg tagccgctgc cgtcttctgc gtggaaatgc
     3421 gggacgttgc ggctgaagct gccgatgagc gagcgggctt tgttgggatt ttcgaggctg
     3481 aacttcggat gctgcaaggc ggtttgaacc tgttgcaggg tgtcgctgcg gcggcttgag
     3541 ccgacgaggg cgaaatattt gtccatcacc agcgcgtcgt ctgaaaactt gtcggcaaac
     3601 tgcgccagca ggcggttgcg cgtatcgctt tcgttgccgt tgacggcgga caggatgccc
     3661 cattcgtggg tcatgttttg cgccatttcg gcgtatttct cggcaacggt ttcgatgtgc
     3721 gcgggatcgg cgcgcaggac gaaggcgcgg cagacattgc gcagcgtgcg ccaaccggcg
     3781 gcttcggggc tgtactcgta gctttggttt tcctgcttcg ccgcctgacg gttcaattcg
     3841 tgccatttcg gcagaaagcg gacggcaagt atatccaaca aggcttcgcg cgcctgatgg
     3901 tagcgcagcg ggtcgatgtt ttccgcgccg tcccacagct cggcttcaga cggcacaccc
     3961 aaaagcaggg ctttgaaagc gttgtctaag aggtcgtctg aaatgacttt ttcgacggcg
     4021 gcaagcagtt tttcgtgttt cggcaacccg atgccgtctg aaagcgcggc aaggttggcg
     4081 gcgacggcac ggcggtagag cgtttgggcg gcttcccagc gcgtgaaggc gtcgctgtca
     4141 tgggcgagca gaagcagcag gtcgtcgtcg ctgtacggat agttcagatg caccggcgcg
     4201 ctgaagccgc gcagcagcga gggaacgacg gcttcggtta cgccttcgag caggaagatc
     4261 tgttcggctt cggtcagcag caatacggct tcggtcgcgc gtttgccctg ataatcgaat
     4321 gccatcgctt cgccgttgcg gttcaacagt ccgactttga cgggaatcat catcggctgc
     4381 ttgtccgcca tatcgggcgt gggcggcacg gtttgtttga tggtcaactc gaaaatattg
     4441 tttttcagac ggctttccgc ttccaaaacg ggcgtgcccg cctggctgta ccacaaggca
     4501 aactggtcga gattgatgcc gttcgcgtcc gccatcgccg cgcggaaatc gtcgcaggta
     4561 acagcctgtc cgtcgtggcg ttggaaatag agcttcatac ctttttggaa gccctcttcg
     4621 ccgagcaagg tgtgatacat ccgcaccact tccgcgcctt tttcataaac ggtcatggtg
     4681 taaaaattgt tcatctcctc atagctggcg gggcgcaccg gatgtgcggt cggacctgcg
     4741 tcttcaggga actggtgctg gcgcagcagg cggatgtttt cgatacggcg cacggcgcgg
     4801 ctggcgcggt cgccggaaaa ttcttggtcg cggaacacgg tcaacccttc cttcagcgaa
     4861 agctggaacc agtcgcggca ggttacgcgg ttgcccgtcc agttgtggaa atattcgtgt
     4921 ccgaccacgg attcgatgcc ttcgaaatcg gtatcggtgg cggtacggct gtcggcaagg
     4981 acgaacttgg tattaaaaat gttcagcccc ttgttttcca tcgcgcccat attgaaatcg
     5041 cccacggcga cgaccatgaa aatatccaag tcgtattcca aaccgaagcg cgtttcgtcc
     5101 cacttcattg cgtttttcag cgattccacg gcaaagccga ccttgggctt gtccgcttcg
     5161 gtggtgtaaa actcgatttt gacgtttctg ccgctcatgg tggtgaaata gtcttccgtt
     5221 accgccaaat cgcccgcgac caaagcaaac agatagctcg gtttggcaaa cgggtcttcc
     5281 catttcaccc aatggcggcc gcctgaatac tcgccgccgt cgattttgtt gccgttggaa
     5341 agcaaaacgg gatagcgttt tttgtccgca acgatggtgg ttgtgaattt ggacatcaca
     5401 tccggacggt cgatgtagaa cgtgattttg cggaagccct ccggctcgca ctgggtaaat
     5461 agattgccgc cggaagcata cagccccatc agcgatttgt tttccaccgg caggatttcg
     5521 gtttccactt caacggtgaa gcgttcggat ggcacgcctg caatcgtcag cgtctcgcct
     5581 tccaacacat aatccgccgc cgccccgtta attttgacgg acaaaagttt cgccgaaccg
     5641 tccaacacca gcggctcccc taccctctgc ggctcaaccg tcaaacgcga cttcacgacg
     5701 gtttgcggtt cattaatatc aaaatgtaaa tcggttttga gaatatggta ggcgggcgtt
     5761 tgatagtctt tgagataatg cacggttttg ctcatttttt tctttcaatg ttattttgtt
     5821 tgactggaaa aggcttcaga cggcacgggc gcatcccgcg tatgccgtct gaagccgcag
     5881 cggcggcacg ggcgcgccgc cggacaaccg gtttgaattc aatctttatt cccacgcgcg
     5941 gacaaactct tcccaatgcg gcttttcccc ggcttgtgcg gacaggtaat tccgcatccg
     6001 tttgatttcc atttcgtatt cgtccgcatc cagcctgccg ctgaccagac agaaacgcag
     6061 gtacatcaga taagtgtttg ccgcgtcggt ttcgcaataa ttgcggattt ccttcagcct
     6121 gcccgtatgg aatgcctccc aaaccttgct gccgtccata cccagcttgc ccggaaaacc
     6181 gcacagtttc gccatatcgt ccagcggcac gtttgccctc ggctggtaaa gcgcgagcaa
     6241 atccatcaaa tcgcagtggc gttggtgata acggctgatg tagttgttcc acttgaaatc
     6301 gcggctgtcg ccgaaatcgc cgtcgcccat atcccaatag cgcgcggcgt tgatgccgta
     6361 tatcagggag cggtaatgca gtacgggcag atcgaaaccg ccgccgttcc aactgaccag
     6421 ttgcggcgta tgtttttcaa tcaattcgaa aaatttagca atgaccactt cctcgccgtc
     6481 atccatctcg ccgatggtgc cgacatgtac tttatcctgc ccccaacgca tgcagcacga
     6541 aatcgccaca acctgatgaa gatgatgctg cataaaatcg ccgcccgtct gagcacggcg
     6601 tttttgctgg gcaaacagca ccacttcatc gtcgggcagc gaggacggca gctcgtacaa
     6661 tgttcggata ccctgcacat cgggtacggt ttcaatatcg aaagccaaaa tcgtggtcat
     6721 gacagcacct tgtatttaaa acggatgcac ctattgtgtc attaaaaggc ggataaaaaa
     6781 agaggcaacc ccccacagga ttgccccaat acctcaaatc agagatttac gcttcacaaa
     6841 caatacaggc tttcgcctgc ggctttaccc gcgtagctca actctacgcc ggcaaacttt
     6901 cgtttcaccg tttccgatga aaccccgacc aatcgcaaga ctgaccggaa aatcctttca
     6961 gacggcattt cctgcctgtc gtgtaattcc atgtagcgaa atgtacgcca ttttctacgc
     7021 tttgccaagc attttttaca atataaatgt caaaacatta attttataaa attgctgaaa
     7081 atattaaata tatggatttt tattttttat atttcaataa atataaattt aattttgatt
     7141 tatatttaaa tttaagcata aaatgtcaaa tattaaagta aacatgaaag gcatatatta
     7201 aatatttatt tataacgcta tgtttttaaa gaaaattaat tttaatatat taactagatt
     7261 gtctgcatat attcataggt ttgcggtatt tcttccaaaa cctgcttcga atttcccgac
     7321 caagtcttaa aaatattgtt tttgagatac ttaaatagca gcgattatca aatgaaatct
     7381 gttcatataa tctgccattt tgcatttaaa aaaacaatca ggagtttcga ctcgaaacgc
     7441 ctgatatgtt ttgtaatttt acgtagtcag taaaaatcgg ggctgccttc cggacgggtt
     7501 ttaaaacgct tgtgcagcca aaaatattgt tccggatgtt cgcgcaccct gtcttcgata
     7561 aaacggttca tgcgctgcgc gtcggctttc gcgtcttcac ccggaaagga tttccaagca
     7621 gggtagaaat gcaatgtaac cgtattgtct gcctcgcgga cgggaatggc gggtatcact
     7681 tttgcatttg caagcgcggc aatgcggctc aatccggtaa tcgttgccgt ctgaataccg
     7741 aaaaaatcca caaaaaccga atcgttgcgt ccgaaatcct gatcgggcag atacagaaac
     7801 ggcgcgctgc ttttgcggaa ctgtttgacg agggcgcgca gcccttcggt gcgcccgata
     7861 aggaagacgt tgtgatagcg gttgcggcct ttcaaaatct gttcgtccaa tatcttgttt
     7921 ttttgatggg aatacatact gatcagcggg atatcctgat taagcgcgta caccgccatc
     7981 tcgaacgcgg taaagtgcgg atacaggatg atgacttttt cccccgccgc cagcgcgtcg
     8041 tccaaataat gcttattgcg gtagcgcacc agcgatttca aacgtccggc aggcgcgtac
     8101 caatataaac cgtattccaa catcagtttc gccatgtgtt tgaaatgctg tttcaacacg
     8161 gttttacgct tttcctcact ccattcggaa aaacattttg ccaaattgat ttcgccgata
     8221 cggcggcgcg gtttgaccag aaggtaggca agcaaacccg tcaggtcggc aatcttgtgc
     8281 agcagcgcaa acggcagaaa ctgcaaaaca tacagtacaa aaaatataaa tttcatctcg
     8341 atacacattt tcttttcaga cggcaaaata caaatgccgt ctgaaactat tgaaacctgc
     8401 cgcgcttgac ctgcatcccc gaaggattga gtttggcggc aagcccgtgg ttgcgtaagg
     8461 cgtgggtcag cgcgacggca agaccgtccg ccgcatccgg ctggggcgtt cccgaaagtc
     8521 ccaacatctg caccaccata tgctgcacct gttcttttgc cgccttgccc ttgccgacta
     8581 ccgcctgttt gacctgcaag gccgtgtatt ccgaaacggg cagcttatgg ctgaccaatg
     8641 ccgccaatgc cgcgccccta gcctgaccga gcatcagcgt cgatgccgga ttgacgttga
     8701 cgaacacctg ttccactgcc gcctgttgag gcttgtaaac ggtaacgact tcgccgatgt
     8761 gccggacgat gacggcaatc ctgtctgcca gaggcgcatc gggaggcgtt ttgatgcagc
     8821 cggaggcgac gtaaaaatga tcgcgccccc tgacatcgat gatgccgaaa cccgttacgc
     8881 ggctgcccgg gtcaatgccc aagatacgga cggttgccga catattcaca acaaaccgtg
     8941 ttgaatcagt tttttacgca aggtattgcg gttcagcccc agcatcacgg aggctttgga
     9001 ctggttgccg ccgcattgct ccatcacgca caccagcagc ggtttttcca cctgatgcaa
     9061 gaccatatcg tacacgccgc aaggttcggt accgttcagg tctttgaaat attgttccaa
     9121 attttgtctg atacattggg aaatatcggg aagggtatgg ggcataatcg cgctttcaaa
     9181 aggataatcg ggtgttcaga cggcattttg gcggtaggcg cacgcccaac ggtcggtttt
     9241 tccggcaagt ctttcaagat aacctgcaag catgtcgtat tgcgccgccg cactgtccaa
     9301 acggttgatt tcacgacgtg tctgttcgcc gtcgggcatt tcgtcgatgt accagcctat
     9361 gtgtttgcgt gcaatgcgca caccggcggt ctcaccataa aacgcgtgca tggcgcggac
     9421 gtggttcaaa atggcggcac tgcattctgc caaactcaag gcaggcggta aaacaccgtg
     9481 ttcggcgtaa tgtttcaaat cgcggaagaa ccacggtctg ccttgcgcgc ctcgccctat
     9541 catgatgccg tcggcggcgg tttgtttgag gacggcggcg gctttttgcg gcgaagtaat
     9601 gtcgccgttg acccaaaccg ggatgttcag acggcatttg gtttcggcaa tcaggtcgta
     9661 agccgcttcg cctttgtaca tttgcgtgcg cgtgcgtccg tggacggcaa gggcggcaat
     9721 gccgcaatct tcggcgatgc gggcgacgac aggcaggttt ttgtggtcgt cgtgccaacc
     9781 caaac
//
LOCUS       DAWHRA010000077         9630 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWHRA010000077 DAWHRA010000000
VERSION     DAWHRA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.077"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            2..202
                     /locus_tag="WIB30_001368"
     CDS             2..202
                     /locus_tag="WIB30_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797432.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
     gene            370..905
                     /locus_tag="WIB30_001369"
                     /pseudo
     CDS             370..905
                     /locus_tag="WIB30_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            938..1129
                     /locus_tag="WIB30_001370"
     CDS             938..1129
                     /locus_tag="WIB30_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797433.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            1187..1843
                     /locus_tag="WIB30_001371"
     CDS             1187..1843
                     /locus_tag="WIB30_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ3797434.1"
                     /translation="MMKFKYVFLLACVVVSLSYRLNAAPMFNDNPVVYGKIKVQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLEKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVGQIRQARAE
                     "
     gene            1969..3264
                     /gene="hisS"
                     /locus_tag="WIB30_001372"
     CDS             1969..3264
                     /gene="hisS"
                     /locus_tag="WIB30_001372"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ3797435.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            3265..3894
                     /locus_tag="WIB30_001373"
     CDS             3265..3894
                     /locus_tag="WIB30_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ3797436.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFAVLILAALGYLGYTVYQN
                     RAASQNQEAAAILAEIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMVAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            4048..5505
                     /gene="der"
                     /locus_tag="WIB30_001374"
     CDS             4048..5505
                     /gene="der"
                     /locus_tag="WIB30_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ3797437.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEEIL
                     ENFPEPEVEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     ERKGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREDRKEEKNRFKKKTKVSVKKQFSK"
     gene            5536..6435
                     /gene="rdgC"
                     /locus_tag="WIB30_001375"
     CDS             5536..6435
                     /gene="rdgC"
                     /locus_tag="WIB30_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ3797438.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     AAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSGRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            6487..6901
                     /locus_tag="WIB30_001376"
                     /pseudo
     CDS             6487..6901
                     /locus_tag="WIB30_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6967..7533
                     /locus_tag="WIB30_001377"
     CDS             6967..7533
                     /locus_tag="WIB30_001377"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ3797439.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(7597..8301)
                     /locus_tag="WIB30_001378"
     CDS             complement(7597..8301)
                     /locus_tag="WIB30_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ3797440.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEAVQDTMSA
                     RLQVAAEGRDKNAVNAEFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAILRFK
                     ARADKLSGILGTSSYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            8670..9053
                     /locus_tag="WIB30_001379"
     CDS             8670..9053
                     /locus_tag="WIB30_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3797441.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTKHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            9085..>9630
                     /locus_tag="WIB30_001380"
     CDS             9085..>9630
                     /locus_tag="WIB30_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3797442.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIP"
BASE COUNT         2612 a         2444 c         2307 g         2267 t
ORIGIN      
        1 aatgcaattg atatgtgccg attggacagg tataggaaat tttatgaaaa ccttcgaaaa
       61 aacatggtct gcacaatatc gggatatgga aatttcagta cggaattttt ggaatttgga
      121 gcggacaggg gcggaagtct atatcaacgg aaggcgggtt tatcataacg aagccgaaat
      181 ggcgtctgct tctttgcgtt agctaatggg ggaatacctg gaatttgaag aaagcggtac
      241 gaaaattacc gttgaaatcg gcagcgcgtg gcattttaat gaaactataa gaaatattaa
      301 tacacattta agaggtacga aacttccagg ttctaactgg agagttcaag ataaggggca
      361 ttttcatctt tggaaaagat aatagattct aaggtaaggg agactaattt taatcaggca
      421 tggcgtgtta tacgcgccat gcaaatagat aatatatccg atgaacaggt tgtgattgtc
      481 agaatgtctt gttatcttaa gaaagatgaa atttttaaga atatagaaag atatggatat
      541 cagtcatcag atattaagaa aattagttct ccttttccat taaatatttt acctgctccc
      601 aaaccttatt atttttatct ttatgatacc catttgaatc aaatttatga tttggcttgg
      661 tcatgtaatg attttttaat gtttgaaata tttattttgg atagggaaag catcaaatat
      721 atagataaag ataaactttt taaatctgga gaaacactat attcgaattt taaatctatt
      781 aaaaatcaat gcaaaaatta tttttttgcg gttttgattt tgatgcccct tactttaaaa
      841 gcggtgtgag tcaggtggca gattataatt ttgttcctga tgcattttct ttattcttta
      901 tttaatcaaa ttatttgaat aagcgtaggg tgggcacttg ctgcccacgc gttctgtttc
      961 aagtttatta ttgggtgaat atttggaatt tgaagaaaac ggtacaaaga ttgctgcgga
     1021 aatcgacagt gcatggcatt ttttgggcgc ggcttgtcgg atttcaataa atggaaagta
     1081 ttatgcgggt aatcggattg tttggtttgc caaaaaagcc gaaagctagg aataatttga
     1141 caagggcagc ctgaaagatt ttcaggctgc cctttatgtg gacaatatga tgaagttcaa
     1201 atatgttttt ctgttggcgt gtgttgtcgt ttctttatct tatcgtttga atgctgcacc
     1261 gatgtttaac gataatcctg ttgtttacgg aaaaatcaaa gtgcagagtt ggaaagcgcg
     1321 gcgggatttc aatattgtaa agcaggattt ggatttttcc tgcggggcgg cttcggtggc
     1381 gacgcttttg aacaattttt acgggcaaac gctgacggaa gaagaagtgt tggaaaaact
     1441 ggataaggag cagatgcgcg cgtcgtttga ggatatgcgg cgcattatgc ctgatttggg
     1501 ttttgaagcg aaaggctatg ccctgtcttt cgagcagctt gcacagttga aaatccccgt
     1561 catcgtgtat ctgaaatacc gcaaggatga tcatttctcg gtattgcgcg gtatagacgg
     1621 caatacggtt ttgcttgccg acccgtcgct ggggcatgtt tcaatgagca gggcgcagtt
     1681 tttggatgct tggcaaaccc gtgagggaaa tttggcaggt aagattttgg cggtcgtgcc
     1741 gaaaaaagcc gagacaattt caaataaatt gtttttcaca catcatccca agcggcagac
     1801 agagtttgca gtcggacaaa tcaggcaagc acgtgcagag taagtcaaat gccgtctgaa
     1861 aactgtttca gacggcattt tacccgtccg caaaccaccc ccaacctcac aaacccttaa
     1921 aaatccgcta taatcgcccg caatttgatt taagcattct tacttgaaat ggcacaaaaa
     1981 atccaatccg tcaaaggcat gaacgacctt ctgcctgtcg agcaaaaaga tttcaaactg
     2041 acggctgcgt tttggcaggc gtttgaggat acggtaaacc gctggacgcg cgcttacggc
     2101 tatcagcaaa tccgtacgcc gattgtcgag caaaccggtt tgtttgtccg ctccatcggc
     2161 gaagaaaccg atgtggtcgg caaggaaatg tacacctttt ccgattcaaa cgattctttg
     2221 agtttgagcc tgcgtccgga aggtacggct tcttgtctgc gtgcggtggt cgaacacaac
     2281 cttctgtaca acagcccgca aaagctgtgg tatatggggc cgatgttccg ccgcgagcgt
     2341 ccgcaaaaag gccgttatcg tcaattccat caggtcggta tcgaggcttt gggttttgaa
     2401 gggccggata tcgatgcgga aatcatcgcg atgtctgccg acttgtggga aaaattgggt
     2461 atccgcgaat acctgacttt ggaaatcaac agcttaggca accgtgagga gcgagcggca
     2521 caccgtgcgg cgttggttga atatttgaca cgctatgaag ataaattgga cgaagacagc
     2581 aaacgccgtc tgaaaaccaa tcctttgcgt gttttagata cgaaaaaccc tgatttgcag
     2641 gaaatctgca acgccgcgcc gcgtttggtg gattatttgg gcgaggcttc gcaaaaccac
     2701 tatgcacgct tcaaggcgat gttggacggt ttgggtatcc aatatattga aaatccgcgc
     2761 ttggttcgcg gtttggatta ttacaatcag acggtttttg agtggacgac cgacaaactc
     2821 ggcgcgcagg caactgtgtg cggcggcggc cgttacgatg gtttgattga ggaacttggc
     2881 ggcaaacctg cgccgtccat cggctttgcg atgggcatcg aacggctgct gcttttggtg
     2941 agcgaatacg gctcgctgga agtgaacgct gcgcctgatg tctatgcaat gcaccaaggc
     3001 gaaagggcgg acttgcaggt gatgaaatac gcgcaagcct tgcgcgcgca aggtttcaat
     3061 gtaatgcagc attccggcta tcaaagcctg aaagcgcaaa tgaaaaaagc cgacaacagc
     3121 ggcgcgcgct ttgccctgat tgtcgcgcaa gacgaactgg cgaacggtac ggttacgctc
     3181 aaagacatga acggcgcaca cggtcagcaa actgtcgccg ccgaggattt aacccatact
     3241 ttacaacaat ggaagaacgc ataaatggca gcccatctcg aagaacaaca agagttagac
     3301 aactttaaat atttttggaa aaccacgggc aaatggctgt ttgccgtgct gattttggcg
     3361 gcgctcggct acttgggata cacggtttac caaaaccgtg cggcttccca aaatcaggaa
     3421 gccgctgcca tattggcaga aatcgtagaa aaggcgcaaa acaaagcccc gcaaagcgaa
     3481 atcaatgccg aattgaccaa actccaacaa agctacccgc attccatttc cgccgcccaa
     3541 gccacgctga tggtggcggc aaccgaattt gacgcgcagc gttacgatgt tgctgaaggc
     3601 catttgaaat gggtgttgtc caaccaaaaa gacagcctga ttcaagcgtt ggcggcacag
     3661 cgtctgggcg ttgtgttgtt gcaacaaaaa aaatacgatg ccgcacttgc cgcgctcgac
     3721 acgccggttg aagcggactt cgcccccctg ctgatggaaa ccaaaggcga tgtctatgcc
     3781 gcacagggaa aaagccagga agccttaaaa aactacggac aggctttaga aaaaatgcct
     3841 caagattctg tcggtcgcga attggttcaa atgaaacttg attcgctgaa ataaatgccg
     3901 tctgaaatgc cgtccattat ttatcagacg gcattttacc ctccctcttt atggcctctt
     3961 gccgtgaaga acctatttac cgtgtccaac ggacacctgc ttgtgttcag ttctcccgaa
     4021 cggcacttga cacagaaaga cccaatcatg aaaccaacca tcgcgcttgt cggccgcccc
     4081 aacgtcggca aatctacttt attcaaccgt ttgacgcgca ccaaagacgc gctcgtacac
     4141 gacctgcccg gtctgacgcg cgaccgccat tacggacacg gcaaagtcgg cagcaaacct
     4201 tatttggtca tcgataccgg aggtttcgag ccggttgtgg acagcggcat tttgcacgaa
     4261 atggcaaaac aaaccttgca ggctgtcgat gaagccgatg ccgtcgtatt tttggtggac
     4321 ggccgcaccg gtctgacccc gcaagacaaa atcattgccg accgtttgcg ccaaagcccg
     4381 cgccctgttt atctggcggt aaacaaaggc gaaggcggca acagggccgt gcttgccgcc
     4441 gagttctacg aacttgcttt gggcgaccct tatgttattt ccggtgcgca cggcgacggc
     4501 gtgtattacc tgattgaaga aattttagaa aacttccccg agccggaagt cgaagaagcc
     4561 gatgcaaaac atcccgtttt tgccgtcatc ggccgtccaa acgtcggcaa atctacgctg
     4621 gttaacgcca ttctcggcga agagcgtgtg attgcattcg atatggcagg tacgacacgc
     4681 gacagcatcc acattgattt tgaacgtaaa ggcaaaccgt ttaccatcat cgataccgca
     4741 ggcgtgcgcc gtcgcggcaa agtcgatgaa gcggtggaaa agttctccgt tatcaaagcc
     4801 atgcaggcgg ttgaagcggc aaacgtcgct gttttggtat tggacgcgca gcaggacatc
     4861 gccgaccaag atgcgacgat tgcaggtttc gctttggagg caggccgtgc tttggtggtg
     4921 gcggtcaata aatgggacgg catcagcgaa gaacgtcgcg agcaagtgaa acgcgatatc
     4981 aaccgcaaac tgtatttcct tgattttgct aaattccact ttatttccgc attgaaagaa
     5041 cgcggcatag acggattgtt cgacagcatt caggctgcct acaacgcggc gatgattaag
     5101 atgccaacac cgaaaatcac gcgcgtcctg caaagcgcga tcgagcgtca gcaaccgccg
     5161 cgtgctggct tggtgcgtcc gaaaatgcgc tatgcccacc aaggcggcat gaatccgccg
     5221 gtgattgtgg tacacggcaa ttcgctgcac gcaatttccg acagctatac gcgctacctg
     5281 acccaaacat tccgcaaagc cttcaatctg caaggcacgc cgctcagaat tcaatacaat
     5341 gtttcagaaa acccctatga aaatgcggaa gacaaaccga agaaaaaacc gctgcgccgt
     5401 gtcagcctga gcaaccgtat tgagaaacgc gaagaccgca aagaagagaa aaaccgcttc
     5461 aagaagaaaa ccaaagtcag tgtgaaaaaa caattcagca aataattccc gacatttcaa
     5521 acaacaggaa acattatgtg gttcaagcag attagttttt atccgctcaa caaagaaaag
     5581 ctgcctgagg cagacgtact tgccgacaaa cttgctgaag ctgaatttac ccattgccaa
     5641 ggcttagact ggttcagcga aggctttgcc gcaccggttt cgttctctcc cgaactcgtt
     5701 ttccctgccg actttacctt gcgcgtcgcc ctgaaaaaag aggaaaaagt cctgcctgcc
     5761 ggtgtcatcc gcgatatttt ggaagagaag gtggcggaaa tccaaaacaa tgaagcccgc
     5821 aatgtcggtc gcaaagaaaa acaagagctt aaagagcaaa ttacagacga cctgctgccc
     5881 cgagcgttta cccgcagcgg ccgtacagag gcggtgttta acacccgcca cggctatctg
     5941 ctcgtcaaca acgcggcttc cgccaaagca gaaaacatcc tgaccaagct gcgcgaagcc
     6001 ttgggcggtt tggaagcctc gctgccgaat accaagcaat cgccctcttc cctgatgacc
     6061 ggctggctgt tgcaagggca ttgcgaaggc ggttttgaat tggacagcga ttgcgaactc
     6121 aaaggtacgg gcgatattgt tcccgtcgtc aaagtatcca aacaagattt aaccgccgac
     6181 gaagtggttc aacacgtcaa aaacggtaaa accgtaaccc agctcggttt ggtttggcgc
     6241 gaacaaatcg cctttatcct cactcaagac tttacactca agcgcatcca atacctcgac
     6301 gtattgcagg aagaagccga aagcaacggc gacgatgccg ccggtctcgc cttcgcctcg
     6361 caaatcctga tggctgaatc cgtcagcacc atgttggaag agctggtttc ctatttgggc
     6421 ggctggcaag attgattttt cagacggctt ttataccgtc agccaaaatc caatggaata
     6481 acccttatgc ttaaacatct cgcattccta ctgcccgcca tgatgttcgc ccccccgccg
     6541 cgtccgccgt tctgacttcc tatcaagaac ccggctgcac ctacgaaggc gatgtcggca
     6601 aagacggtaa acccgccggc aaaggcacat gcgctgccaa gacgggcgca actataccgg
     6661 ttcgtttaaa aacggcaaat tcgacggaca aggcgtttat accgttgccg ccaaccgcga
     6721 aatatttatc gaaccgttca attccgacag taccaaattc cgcaacatgg tactctcggg
     6781 cacattcaaa aaaggcttgg cacacggcag atttaccgtc tcgcaaaacg gcgaaaccct
     6841 cttcattatg aaatgcgaaa acggcatgat taaagaagtg aagctgccca aaaacaaata
     6901 agccccgttt tcagacggca tccctgaatg ctttgtccga acactgcaag aaacaaggaa
     6961 aacattatga gcatcaagtc cgacaaatgg atacgccgaa tgagcgaaga attcggcatg
     7021 atcgaccctt tcgagccgaa ccaaatcaaa gaagccgacg gcaaacgcat catctcctac
     7081 ggtacgtcca gctacggcta cgacatccgc tgcgcaaacg aatttaaaat cttcaccaac
     7141 atcaacagca ccatcgtcga tcccaaaaac ttcgacccga aaaacttcgt taccgttgaa
     7201 gacgactgct gcatcatccc gcccaattcc ttcgcactgg cgcgcacggt cgaatatttc
     7261 cgcatcccgc gcaacgtcct gaccgtctgc ttgggcaaat ccacctacgc ccgctgcggc
     7321 attatcgtca acgttacccc gttcgaaccg gaatgggaag gctacgttac cctcgaattt
     7381 tccaacacca cccccctgcc cgccaaaatc tacgcaggcg aaggcgtggc gcaagtcctc
     7441 ttcttcgaga gcgacgaaat ctgcgaaacc tcatacaaag accgcaacgg caaatatatg
     7501 gggcaaaccg gcgttaccct gcccaaagcc tgaaatataa atgccgtctg aacccttatc
     7561 gtcgggtttc agacggcatt tgccgttcac ctcaaatcag aactgaaccg tcccattgac
     7621 gctgatgctg atttcctcca caccaggcgc ggcggaatcc gcaccctcca tattgacgct
     7681 tgccgccatc ggcatggcac gaagcatttt tgcctgagca gctccccctc ccgcgatatg
     7741 gctgccgatg tgtcccaaat tcaatttgac gattttataa ctggacgtac ccaatatgcc
     7801 cgacaacttg tctgcacgcg ccttgaaacg caaaatagca tctttactga cctgatcgat
     7861 gacctcgttt cggcgttcgc gcgacacatg gaaatccgta tattccaatg cggcatctgc
     7921 ctgaacatcg gcaataaaac ggtttaactc atcaaaatcc ctcccttcga ccttaaattc
     7981 cgcacgctcc tcccaacctg tttgaatgcg tctgccgttg gtatattgat agcgcggcat
     8041 cgcactgcgc gataccaatt cggttttaaa gctaccattt ttcgattttc tgatgaactt
     8101 gttgaatttt ttaacaaact cggcattgac ggcatttttg tcccgtcctt ccgccgccac
     8161 ctgtaaacgc gcggacattg tatcctgaac cgcctcgaca ccggccgatt cggaaaattc
     8221 gacaatattg taattcaatg cttcagccgc tgccggaaaa gataccgcca atagggaagc
     8281 cgccaaaata gaacgcagca tatccgtatc cttacttata aaaccgccaa tctagcatat
     8341 cggcgcgcta aattgcgtta gcgtaaggca acaatttaaa tattcggtct gcgtctgtct
     8401 tataataacc gcctttccga tacggaatcc cactatgccc aaacccttgc acgtcctcgt
     8461 catcccctca tggtatccgc aatccgaaca ggatgtggac gggatttttt tcaaaatcag
     8521 gcactggcat tgcagaggaa aggcatcaaa accgccgtgc ttgcaccgat gttccgctac
     8581 ttgcggaaag aaacagcaag catcctgacc ggccttacgg ttttgcaaaa tatcggcaaa
     8641 gcggtttgga catctatgca tggcacggca tatatttctt cccccgcttt ccgttcatcg
     8701 acatcgactg catccgctgg gtgtgtgcgg gtttgaaagc tttcaaacac tacatccgcg
     8761 aaaacgggct gcccgacctg attcacgccc accgcatgaa ctatgccggc atactcgccc
     8821 aaaaaatttc ccaaaaatac ggcatcccct atgtcgtcac gaaacacagc agcaccatca
     8881 cgcgcggtct gatccgtccc caccaatggc agccgatgga aaaagcagcg gcgcacgcct
     8941 ccgcactgct cgctgtcagc cgccatttcg cacacgtcct gcaacacaaa tacggctgcg
     9001 aatggcagta cctcccccaa catactgggc ggaatattca accgaccgtt tgaacaaaga
     9061 gaaaaaaaca acaaaccgca tttcgtgttc tgcaccgtct cgcaccttcg ccgtctcaaa
     9121 ggacacgatg tcctactcac tgcctttgcc cgggcgttgg cacaatgccc gcaactgcgc
     9181 ctgaacatcg gcggcagcgg acaggaagaa cagcggctga aacagcaggc ggcagacttg
     9241 ggcattaccc atgccgttac atttttgggc gcattgcagc ccgaagcagt cttggatttg
     9301 atgaggaaca gcgacgcatt cgtccttgcc agccgcaccg aaaccttcgg cgtggtctat
     9361 atcgaagcac tgtcccaagg attgcccgtc attgcgacac gctgcggcgg tgcggaatct
     9421 attgtttcag acggcaacgg atatttggtt cctgttgacg acgacgatgc ccttgccgac
     9481 gcactcatca aaatgtatga acaccactct gattttgaac ctgcccgact tagggaaaac
     9541 tgtctgaacg aatttggcga aaatgccgtt ataggcaggt tgatcggcat tttccgacag
     9601 gcaatcgcag aatacggtaa gaaaataccg
//
LOCUS       DAWHRA010000078         9440 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWHRA010000078 DAWHRA010000000
VERSION     DAWHRA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.078"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(61..1119)
                     /gene="dinB"
                     /locus_tag="WIB30_001381"
     CDS             complement(61..1119)
                     /gene="dinB"
                     /locus_tag="WIB30_001381"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ3797443.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(1263..4352)
                     /locus_tag="WIB30_001382"
     CDS             complement(1263..4352)
                     /locus_tag="WIB30_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ3797444.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALLGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGMTVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILPCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEYAAGLSGEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDKCMSDPFALDFI
                     CQGSWGDPGVDADKAEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRYRVRPNT
                     GATHGVYAGSCQEEPDGDLSSPLVRGHKEPDWQAYDEKGNRTVYAEECRNAKKIKTEP
                     KLDAEGKQVYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGTPAFTGFSGTVPVWK
                     TVKVADDHVPALYNYAKYLNTNKTHSLTASTRFNVTGRLHLLGGLHYTRYETSQTKDM
                     PVRYGQPASDFQTASSIRADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGSYTKIFK
                     QQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVDFGYVPG
                     AGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKNAAEVNAER
                     LAKNSSADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNIRQGGYG
                     LIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            4626..5405
                     /locus_tag="WIB30_001383"
     CDS             4626..5405
                     /locus_tag="WIB30_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ3797445.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGNTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALTEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(5616..7028)
                     /locus_tag="WIB30_001384"
     CDS             complement(5616..7028)
                     /locus_tag="WIB30_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ3797446.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKVKAELVGRYHSDCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            7119..7195
                     /locus_tag="WIB30_001385"
     tRNA            7119..7195
                     /locus_tag="WIB30_001385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7153..7155,aa:Arg,seq:acg)
     gene            7213..7287
                     /locus_tag="WIB30_001386"
     tRNA            7213..7287
                     /locus_tag="WIB30_001386"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7246..7248,aa:Glu,seq:ttc)
     gene            7846..9021
                     /locus_tag="WIB30_001387"
     CDS             7846..9021
                     /locus_tag="WIB30_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ3797447.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
BASE COUNT         2378 a         2602 c         2137 g         2323 t
ORIGIN      
        1 gccatgccgt ctgaaaaccg ttcagacggc atattttttg gacggcgcgc gtaaagcggt
       61 ttacgcccac aaatcctgct gctggttttt cggcacaaga tgccccacgc cgataccgat
      121 aaggcggaac gcgtcttccg tctgcggcgg gacgcgcgcc atcaacattt gcgcagcctg
      181 cagcagagct gcgcagtcgg gcaatacgga ggaataagtc agtgtgcgcg tgatgatgcg
      241 gaaatcgtag gtcttcagct tgagcgttac gctttgggct tcgacgtttt tgcgcgtgat
      301 ttgccgccac aagtcttcgg caagatgggg gaggtgtccg gcagcctgct cgagcggcag
      361 gtcttcgggc agggtaattt ctgtggagat ttggaggcgt tcgcgttcgg ctttgacggg
      421 gcgttcgtcc gtaccgcgca ccaaatcata gaggcggtat ccgtagcgtc cgaaatggtt
      481 taagagttcg ccgcgctcga aacggcgcaa gtcgcccgcc gtccgcatac ccagcgactg
      541 catttttttc agcgttacct tgcccacgcc ggggattttg cccaaaggca gggtttccaa
      601 aaatgccatg actttgtgcg gcggcaacac aaactgcccg ttcggcttgc gccagtccga
      661 cgcgattttc gccagaaatt tgttcggcgc gatgcctgcg gatgcagtca aacctgtttc
      721 cgcaaaaatg gcggcacgga tttctttggc aacttcactg gcgtaaggga tgtttttgaa
      781 attacgggta acgtcaagat aggcttcgtc cagcgacaag ggttcgatta aatcggtata
      841 acgcctgaat acggcgtgaa tctgcgcgga aacctgacgg tacaaatcga aatgcggcgg
      901 cacatacacc gcttgcggac acagcctttt cgccgttgcc accgacatcg cggaatgcag
      961 cccgaactgc cgtgcctcat acgatgcggc gcaaatcacc gaacgcgcgc cctcccacgc
     1021 gacgaccacc ggccgccctt tcaaatgcgg ctgttcgcgc agctctaccg atgcgtagaa
     1081 tgcgtccatg tcgatgtgga taattttgcg tgaagacatc ggctcttctg aggataaaag
     1141 ggatattcta ctgccggcat cgggcaaatt ccaaatatac gccccgatag acctgcctcc
     1201 ataaaaatgc cgtctgaaac ataccctgtt tcagacggca tccgcaaaac tacggttttc
     1261 aattaaaact gccaatccag tttcatgctg acagtgcgcg gctctccgta gaagttgttt
     1321 gcgccgcgcg tacggttgta gttgttctca aaataagtgc gtccgtttaa gttcgtaccg
     1381 atgaggctca atttggcgtg tttgcccaat tcgtaacgga cgaaaccgtc tatcagcccg
     1441 tagccgccct gcctgatgtt atacagactg cttgtgccgc tttgtgcgga cacgccgccg
     1501 ccgacggtca gccccgtatt cggtatatgg aagctcgttc cgaaacggaa tatgtgcacg
     1561 ggtgtgaaat tgctgaagtt gtacgggtct gcactggaat ttttggcaag gcgttcggcg
     1621 ttgacttcgg cggcgttttt gtagcggctc ttgttgtagg tgtaacccgc aaagactttc
     1681 caatcttcgt tcaactcacc cgacaactcg aattccgcac ccctgctgac cactttgcct
     1741 atcggtttgg caacggtttg gaacgacccc tgcttgccgc ctgctccggg aacatagccg
     1801 aaatcgacga ccgtgcggtt tttctgttcg aggtaaaaca atgcgaacga agcattcagc
     1861 cgtccttgca agaacgcgcc tttccagcct acctcatagt ttgtgccgac caaaggcggt
     1921 aaaacggttt tggcactgac atcgacatta tcctgctgtt tgaagatttt ggtataactt
     1981 ccgtaaatac tctgttgcgg tgtcaagtca taggtaatgc ctgcataggg cgtcaattta
     2041 tgaccttgca tcttggccgt gtaatggtcc tgatccgccc taatgctcga tgccgtctga
     2101 aaatcgcttg ccggctgccc atagcggaca ggcatatctt tggtttgcga agtctcatag
     2161 cgcgtgtagt gcagcccgcc caaaaggtgc agtcggccgg ttacgttgaa acgcgtgctg
     2221 gcagtcagcg aatgggtttt gttggtgttg aggtatttgg cgtagttata cagcgcagga
     2281 acatggtcgt ctgccacttt gacggttttc caaaccggca ccgtaccgga aaaaccggta
     2341 aaggcaggcg tgccgtcggg attggtctcc tgaatcttgt tgcctttttc gtccagctca
     2401 tatacatcga catataccgg tgtccggctg ccgctgtatt cgtcatagta atacacctgc
     2461 ttgccttcgg catcgagctt gggctcggtt tttattttct tggcgttcct gcattcttcg
     2521 gcataaacgg tacggttgcc tttttcatcg tacgcctgcc aatcgggttc tttatgcccc
     2581 ctgaccaaag gagacgacaa atcgccgtcc ggctcctcct gacaacttcc cgcatacacg
     2641 ccgtgcgttg cccccgtatt cggacgtact ctgtagcggc gttcgtagat ttctagatat
     2701 tccgaacgta tcttttcatc accgtaggca tagccgacaa agaaatcatg ctcccgcccg
     2761 aacagcccat atgtgccggt caggtcaagt ttaattcccc attggcggtc gtctttggta
     2821 tgccgcaacg gcatataact gtatcgtcgg ttggcggtag ccttccgatt aaaggaagag
     2881 tcatacaggc tgtttggaaa acgttgtgcc gcattattaa agatgccctc cttcgcaagg
     2941 gctttatcga caaattccgc cttgtcggca tcaacgcccg gatcccccca agaaccttga
     3001 cagataaagt ccagcgcgaa agggtcactc atacacttgt caaaaccggc tttgcgttct
     3061 gcggcacggc ggctgcgata ctgttcgaaa gcagtattat cgaaacggtt tttaacaaaa
     3121 tcgtctttgc gctcccggta ttccttggcg gtttcatcac gatatgcttt cagtttctcc
     3181 aatgccttat ctttcggctc gaacgggatg acttcgtttt tttcagtcaa aaagcctacc
     3241 gcatcctcac ccgacaaacc cgccgcatat tcgtttttca gaaaaaactg ccccaccttc
     3301 gcatcggatt cattcttggt ataagacact tcggcattga gctgccaacc gttgtcaaac
     3361 acatgtttga atcctgagaa aaggttgtat ttgtcggcac ttaaccgcga ccaatcctcc
     3421 cccaaataag tgttgcgcgg cagttgcaaa ggccggttgc aggcaggcgt tgaactgaac
     3481 ggggcagttt tctgattttc acagggcaaa ataatgcccg aaaaatcagg aacctcccta
     3541 ctcttctgat acatgccgcc caaagtaagc acactgctgt cgcccgcatc ggcttcggca
     3601 atgccgtaaa ccatatgttt cctgccccaa acgcggtctt taaacgattt tttatactct
     3661 tccgcaccca ccaaccttcc gcgtaaggta ttcgccttat tcaggctgcc tgaaacatcc
     3721 aacactgcac gccggctgcc gcgatggtcg gcggtcagct ctccggtatg tttgaaagaa
     3781 gcggtaggtc gcttacggat caaattgacg gttcctcccg gctctgaatt ggattgggtc
     3841 aaccccgttg caccccgtac aacttcaata tggtcataaa ccgccaaatc ggtactcgga
     3901 gacacgtcga ttttcgccgt atatcccgaa cggcctgcaa cattgacggt cataccgtct
     3961 tcaccaatct gatcaatata gaaaccgcgt gacaaaaacc gcgtctgcaa gcctgaatcg
     4021 cgcacaacgt tgacacccgt cgtgtttttc attgcctctt caagcgtatg caccgcctta
     4081 tcgtcaaggc ggctgcgcgt gatgacgctg accgactgcg gcgtatcctt gcccgcaatc
     4141 ctcatacctg tggcggtgga catccgatct atcgtataag aacgggtctt ttcggtcttg
     4201 cccaacaaag catgagagcc gcgtacattg accgtatcca gactgacggt attgccgtct
     4261 gaaacaggca caacaccgtc tgcaaaagaa ccaccgtaag ccgataacag cataacggtc
     4321 agaattttaa gtgaaaaatg attttgattc atagagacct ctgtaatatg caagtgtgca
     4381 aatcgtccaa aggctctcac aactgttttg attttttata ttaattgaaa aaaagtaatt
     4441 ctcaattaaa tttatagata ggattgtatt cccattttga caaaaaacaa actactcctt
     4501 accgtttatt tcaaaaaacg ataacattgt attgaaaaat atccgaattt aaatacagac
     4561 cgccaatgca gaaaaaaaca cccaaattgg ctataatccc gacaaacaca ctcaaggaca
     4621 acaacatggc agcctcgccc gaagcaaaat tcaccgaaga aaagattttg tgggtcaaac
     4681 accacacgcc gaaactcatc actttcgcca tcagccgtcc cgaatcctac cgctttaaag
     4741 ccggacagtt ctcccgactc ggtttctacg aaggggaagg tttcatttgg cgtgcctatt
     4801 ccattgtttc cgcagaatat gccgacacgc tcgaatattt tgccgtactc atccaagacg
     4861 gccccatgtc ggcccgtttc gccaaaatgc aacagggcaa caccatcctg ctcgataaaa
     4921 atgccaccgg cttcctcctg cccgaacgct tccccgacgg caaggatttg gtgatgctct
     4981 gcaccggctc gggcatcgcc cccttccttt ccattctcga acaacccgaa atccgtcaac
     5041 gtttcgatac cgtcaacctg atacattccg tatcttttcc cgaagaattg attttcaacg
     5101 accgactcgc cgcattgact gaacatcccc tggtaggcga atacggacac tctttccgtt
     5161 tcgtccctgt taccacccgt gccgccaacc cctcgggctt aagcggaaaa cgcattccgg
     5221 aactcttaaa aaacaacagc atcgaacagg cgctgcatac caagttcacc ccggaatcca
     5281 cacggtttat gatttgcggc aacccggaaa tggtcaaaga cactttccaa acgctgctcg
     5341 acatgggtta cgccatgcac cgcaaccgca ttcccggtca aatcatgatg gaaaacggct
     5401 tctaaaaacc accctgcttg tccgatgcct tcggatggac gggcaaaccg acacggcacg
     5461 aaaaccgcgt cggcaaaaat gccgtctgaa aaaattcaga cggcatcttc ggatacatta
     5521 cctgcaaacg gcaacacacc ggcacaaacc gattaggcaa tcaacacggt gacggctgtt
     5581 tacatacttg ccggctttca ccaaccgata tcgatttaac cgatttcctt aatatttttc
     5641 ctgtccgttt taaacttcgc cttaaacgca tccggtaaat ctttatcgaa ataccaaagc
     5701 ccgtcatcca tttccaatgc gccgcccatt ccgtgcagaa cgactttttc ccccgtcaaa
     5761 ggatcggttt cggtaatttc cacctcatgc atttttcccc aatccaaaac aggcaacttg
     5821 tgtgcaaacg ccaaaacctg ttcgtcagaa cggccgcacg ccttatcgca tcggcctgaa
     5881 acatatacgg gacaaggctc atcctctgca taaccgtccg gaaggatacc ggctgcggca
     5941 ggacacaatc cgtatgtttc cgcccacgac gacaaagccc gtctcgctgc ctttttattg
     6001 gcaaataatc cggtaggcgg attatccgtc acaccgtttt tcaaagccgc tgttttcgca
     6061 ttcaacatgc cgtctgaacc tttttcaaac ctgacggtcg taaatgtttt aagcagattt
     6121 ttggcagaca cataacaatc cgaatgataa cgcccgacca attccgcttt aaccttatat
     6181 gcatgcaggc tgcccaatgc gggaaaaaaa cggacttcct ccgtattgca ccaatcaaac
     6241 ggggcttttc cggagtccaa taaagccgaa acctcgctat atacccgttc aaacgtaccg
     6301 atataattta ctttccctcc gccgtcgaaa caagccagca cccccatacc gtcaggcaaa
     6361 ccgtacaact gttccctcaa ccgttcgggc agcgcggcag gcagcggttt cggattcatc
     6421 aaacggaaac actgcctgat ccatgcctca accccgtgtt ccgacagact gtattccaaa
     6481 taatcacaca atgccgatac atccgccatc gcacgatgcc tgtcttccac aacaatcccc
     6541 aacctttcga tgatactgtc caggctgtgc ttgtaaaatt gcggatacag acaccgggac
     6601 agctgcacac tgcacaaagc aggcgatgaa aatccgatac ccgcacgatg aaactcatgc
     6661 tttaaaaacg tatagtcgaa acggctgtta tgtgcaacca gcacacaacc cttcaatacc
     6721 gaaaacaact cgccggcaat ctctgcaaaa acaggcgcat cggcaaccat gccgtctgaa
     6781 atccccgtca gccccgccac aaactgcgga atcggttttt gaggattaac caaccactca
     6841 tgcctcacca cccttccctg ctcaaacttg accaaagcca cttcggttac cctgtcttca
     6901 tacagattgc cgcccgtcga ttccaaatcg accacggcaa caggcattcc aaaccgtaaa
     6961 aatacctttt ccagcaaggg ccagcgggaa gcaacaatca ttttattctc tttaaattca
     7021 aacaacaaac cgatatttta cacttttaag gcatttcatc caacaaaaca attgacagaa
     7081 tccgatgatt accctaaaat tcaaatcttt cttgcagtgc acccgtagct cagttggata
     7141 gagtatctgg ctacgaacca gagggtcggg cgttcgaatc gctccgggtg cgccagtaag
     7201 aaaatacaat atgcgcccat cgtctagcgg ttaggacatc gccctttcac ggcggtaacc
     7261 ggggttcgat tccccgtggg cgtgccaaat tccaaatccc ccgaaataat ccctcgggga
     7321 tttttattgc ctcggcaatt cattaccata tctttaccta cccccttcat cagactctca
     7381 gacgtaatcg aatcatattc aaacctttgc cgtgcaaacc gatatcacgt aaccggatgc
     7441 ggtgtccgtc caacatttta cccgattgaa acgcctgata tattgcaccc catcaacgtg
     7501 gcattacttt tcttaacaat cccctttgac agcaactgac tagggctttt ttatgccatc
     7561 atcaaattta tagtggatta actttaaacc agtacggcgt tgcctcgcct ttccgtacta
     7621 tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaattcac tataatattt
     7681 tctctcccga ttgaaacagg cgtaacagaa tatgcccgaa gctccggctg ctttcttgtt
     7741 taccgccgcg atatttagag tataatacca aatttgagca atagttctaa aacagttaga
     7801 accatttttc atgagcctga ctgattcgta tactcggaga aactgatgca aaatattttt
     7861 gaccctttgg ttattcgtgg aaaatccctt acccccatcg tgcaaggcgg tatgggggtc
     7921 ggtgtttccg catcgggttt atccagcgcg gtggcacgtg aaaacggtat cggaacgatt
     7981 gccagtgtgg atttgcgcca ccttcacgaa gacctactcg ccgaatcaca aatcaatccg
     8041 agtgaagaga aatatacatc tttgaactgt accgcattag acagggaaat ccaaaaagcc
     8101 aaaagcgctt cagagggaaa aggactgatt gcggtcaacg tgatgaaggc ggtcaaagac
     8161 cacgccgcgt atgtccgcca ggcttgcgaa tcaggggcgg atgcggttgt aatgggtgcc
     8221 ggcctgcctt tagacctgcc ggaaatgacc gagggctatc ataaagatgt cgcgctgctg
     8281 ccgattctgt ccgaatcgcg cggtattaat atcgtcttga aacgttggat gaaaaaaggc
     8341 atattgcccg atgcgattgt agtcgaacat cctgcccacg cggccgggca tttgggcgcg
     8401 tcaaccgtcg aaggcgtaaa cgatgctaaa ttcgacttca aacgcgtgat tgaggaaacg
     8461 tttgaagttt tcaaaagttt agggctggaa agcgaaaaaa tcccgcttat tcttgcggga
     8521 ggcatggcaa attttgaaaa agtcaaaacc gccctaaaga actggggagc atccgccgtt
     8581 caaatcggta cggcttttgc cgttaccgaa gaaggagatg cacaccttaa cttcaaaaaa
     8641 acgctcgccg gtgcggaaac tgaaaaagta gtcgaattta tgtctgttgc cggtttgccg
     8701 gcgcgcggtg tccgcaccaa attcctagac agctacatca agcgtgaaag caaacttcag
     8761 acaaacgcca aagccgaccc gcgccgctgt acccaaggtt taaactgcct aaccagttgc
     8821 ggtctgcgcg acgggctttc caaagccgga caattctgca ttgatatcca gcttgccgcc
     8881 gcattccgtg gagaagtaga taaaggcctg ttcttcagag gtaaagaccc gctgcccttc
     8941 ggcaatgcca tccgcaccgt ccgcgagacg atacaatatc tgctgacggg gagcgaacct
     9001 gttgcaacgc tcggacgctg acatcaggta ggatttgatt tgcaaacaaa atgccgtctg
     9061 aagcctttca gacggcattt ttcaggctgc gtttcggaat agtgtcgaaa aaacggagag
     9121 aaataaaaac gtttttatgt aaacaaaacg ccttccgcat tgtgtcgggc atacaacaca
     9181 aatgccgtct gaagcccttc agacggcatt tttcaggctg catttcggaa tggtgttaaa
     9241 aaataaacgg gataagatac atttattttc gtccgataaa tcagaccatc acgccgaata
     9301 atcaaacaat gcctgcccga cattgcatct ggcaaaccaa tgcaatgaaa acacggcttt
     9361 tttatttgct tccggcatta ttgaaaagtt tatccatcgg ggtcagatcc accgcattcc
     9421 cttggctggt aatccatttg
//
LOCUS       DAWHRA010000079         9434 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWHRA010000079 DAWHRA010000000
VERSION     DAWHRA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.079"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(135..470)
                     /locus_tag="WIB30_001388"
     CDS             complement(135..470)
                     /locus_tag="WIB30_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ3797448.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            599..1504
                     /locus_tag="WIB30_001389"
     CDS             599..1504
                     /locus_tag="WIB30_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ3797449.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGNGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1566..2054)
                     /locus_tag="WIB30_001390"
     CDS             complement(1566..2054)
                     /locus_tag="WIB30_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ3797450.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2201..3103
                     /locus_tag="WIB30_001391"
     CDS             2201..3103
                     /locus_tag="WIB30_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3797451.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3144..4274)
                     /locus_tag="WIB30_001392"
     CDS             complement(3144..4274)
                     /locus_tag="WIB30_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3797452.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4478..7447
                     /gene="alaS"
                     /locus_tag="WIB30_001393"
     CDS             4478..7447
                     /gene="alaS"
                     /locus_tag="WIB30_001393"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ3797453.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYVSDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQKQAFFYKLV
                     PDLVKAMGDAYPELKEKQTQIMEALRAEESRFGETLEKGMGLFNQVYNELKFNQIFDL
                     LAKEFKVTPVEDIDLDMFSTRNFVGRSAEIPYISNQHPNVDYPAPMGNVNEKIYAMPV
                     RLTDGRIGINIFPTDETARLILSNDWNSLRFYSFCNSLITYYQQKGQLETLKLNGEHI
                     FKLYDTYGFPYDLTADMARELGIELDEAGFEREMEAQRARARAAQSFKANAQLPYDGQ
                     DTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGYI
                     FSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATHL
                     MHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAVN
                     AAIMSMEDAQKTGAMMLFGEKYGEEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIISE
                     GGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKAL
                     EKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNAV
                     ILLAAVNDGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDADK
                     LPAVLDSVKDWVGAKLV"
     gene            7708..8139
                     /locus_tag="WIB30_001394"
     CDS             7708..8139
                     /locus_tag="WIB30_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797454.1"
                     /translation="MSISLIGLHITIAIILFFTTNFMGKKSSIFGYYQLSFSEENHSP
                     AFNIFYRAFTPILFIVIFSWVVTSLEIPISLEKINYVVIYYFIIRLLSVFVFEKTHIV
                     NWFNQLTIPILSITLSFIVYNKMILPKSFLLPSSQEVATTF"
     gene            8439..8726
                     /locus_tag="WIB30_001395"
     CDS             8439..8726
                     /locus_tag="WIB30_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797455.1"
                     /translation="MQVTSAEYLSNEESIKKGGNILMEKYNEKYNESIDGNKTLYKSY
                     YESRRESIKNYNPDAKYINEIESIYMMLGEIYPNAPDFMSPHFEGDCSEGE"
     gene            8925..9011
                     /locus_tag="WIB30_001396"
                     /pseudo
     CDS             8925..9011
                     /locus_tag="WIB30_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            9214..>9434
                     /locus_tag="WIB30_001397"
     CDS             9214..>9434
                     /locus_tag="WIB30_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ3797456.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLS"
BASE COUNT         2459 a         2381 c         2220 g         2374 t
ORIGIN      
        1 ttcacgctta ccccttgttt ctcaaatgcc gtctgaaaaa aacggctgta tatgttgtct
       61 gcaaaatatc gggaagcata acagacaaaa tgccgtctga aatattttca gacggcattt
      121 caatccgatt cggattattt ttgcgtttca accgcttcca atgcacgcag ggcgtaagtg
      181 taagcggcac cggcattcag ggcgatggcg gttgccaatg cgccggcgat ttcgctgtcg
      241 gtcgcaccgg ctttggcggc ggcggcggcg tgaacactga tgcagctctc acaacgtgtg
      301 gtaatggcaa cggcgatggc aatcagttcg cgcgttttgg catcaagtgc ttcggcggcg
      361 gcggcctgtt ccaatgcgcc gtaggcctgc agcattttag gatgcgcctt acccagctcg
      421 ccgaacgatt ttttaaccaa tgcggtatgt tctttccaat ctttaaacat tttcttttcc
      481 tttctcttgc gtttaaccct gatacgcgct tgcgtatctg ttttcgatgt gcgtattatt
      541 gcaattattc agttgtgttt ctcgtttaat catctcattt tatggttcaa aaagatttat
      601 ggacattctg gacaaactgg tcgatttcgc ccaattgacg ggcagtgtgg atgtgcagtg
      661 ccttttgggc ggacaatggt cggtacggca tgaaaccttg caacgcgaag gactggtaca
      721 cattgttaca tcgggcaacg gctatctctg catcgacggc gaaacttccc cgcgtccggt
      781 cagtacaggg gatattgtat ttttcccgcg cggcttgggt catgtgttga gccacgacgg
      841 aaaatgcgga gaaagtttac aaccggatat gcggcagcac ggtgcgttta cggtcaaaca
      901 gtgcggaaac ggacaggata tgagcctgtt ttgcgcccgt ttccgctacg acacccacgc
      961 cgatttgatg aacgggctgc ctgaaaccgt ttttctgaac attgcccatc cgagtttaca
     1021 gtatgtggtt tcaatgctgc aactggaaag caaaaaacct ttgacgggga cggtttccat
     1081 ggtcaacgca ttgtcgtccg tcctgctggt gcttatcctg cgcgcctatc tcgaacagga
     1141 taaggatgtc gaactctcgg gcgtattgaa aggttggcag gacaaacgtt tgggacattt
     1201 aatccaaaag gtgatagaca aaccggaaga cgaatggaat gtcgacaaaa tggtggcggc
     1261 tgccaatatg tcgcgcgcgc aactgatgcg ccgtttcaaa agccgggtcg gactcagccc
     1321 gcacgccttt gtgaaccata tccgcctgca aaaaggcgcg ttgctgctga aaaaaaaccc
     1381 ggattcggtt ttgtcggtcg cactgtcggt aggctttcag tcggaaacgc acttcggcaa
     1441 ggcgttcaaa cggcaatatc acgtttcgcc gggtcaatac cggaaagaag gcgggcaaaa
     1501 ataaatcggg gcttcaaacg caaatgccgt ctgaaaaggc tttcatacag catttgcgta
     1561 ccgcgtcatt tcaagggctg catcttcatc acttccatca aaaagttggt aaatgcgggg
     1621 ttgttgggtt tgacatccat atttttccaa cgctgctgcc agccgcgcaa ggcattctgg
     1681 atatacagct tggactgttc cgtattgatt gcgccccgct ggctgtctat cgccgaacgc
     1741 aggtagattt catacatact gtcatcgacg gcattgcgtc cgaccaggcg ttttctgaag
     1801 ttgttcagat attgcgccgc ctgaaccttg gtcattttac cgatacccac ctgatagccc
     1861 aagcgcgtcg cttcatcgct gattttggca acatccgtcc aatgcgaaga ggcaaggcgg
     1921 aaaccttttg caggtgcttc cgttttgacg gtattgatag gattcacggg gatttccgtc
     1981 aatgtgggca cataaataga ctggcagccg gaaagaactg ccacaatgga aagagggata
     2041 aggtattttt tcatgccccc attataatca agtttgcctt gagaaaacaa attgttcggc
     2101 aagaaaaata aaatttcggc atcagaagca ggcaaaaaca cattccacaa gccttgccgc
     2161 aaggtttaca atccgaccgt ccttatcgca acgaccgttt atggataccg caaaaaaaga
     2221 cattttagga tcgggctgga tgctggtggc ggcggcctgc tttaccatta tgaacgtatt
     2281 gattaaagag gcatcggcaa aatttgccct cggcagcggc gaattggtct tttggcgcat
     2341 gctgttttca accgttgcgc tcggggctgc cgccgtattg cgtcgggaca ccttccgcac
     2401 gccccattgg aaaaaccact taaaccgcag tatggtcggg acgggggcga tgctgctgct
     2461 gttttacgcg gtaacgcatc tgcctttggc caccggcgtt accctgagtt acacctcgtc
     2521 gatttttttg gcggtatttt ccttcctgat tttgaaagaa cggatttccg tttacacgca
     2581 ggcggtgctg ctccttggtt ttgccggcgt ggtattgctg cttaatccct cgttccgcag
     2641 cggtcaggaa acggcggcac tcgccgggct ggcgggcggc gcgatgtccg gctgggcgta
     2701 tttgaaagtg cgcgaactgt ctttggcggg cgaaccctgc tggcgcgtcg tgttttacct
     2761 ttccgtgaca ggtgtggcga tgtcgtcggt ttgggcgacg ctgaccggct ggcacaccct
     2821 gtcctttcca tcggcagttt atctgtcatg catcggcgtg tccgcgctga ttgcccaact
     2881 gtcgatgacg cgcgcctaca aagtcggcga caaattcacg gttgcctcgc tttcctatat
     2941 gaccgtcgtt ttttccgctc tgtctgccgc attttttttg ggcgaagagc ttttctggca
     3001 ggaaatactc ggtatgtgca tcatcatcct cagcggtatt ttgagcagca tccgccccac
     3061 tgccttcaaa cagcggctgc aatccctgtt ccgccaaaga taaaaaatgc cgtccgaaca
     3121 tccttcagac ggcatatcgg gctttatttc cccgccttca catcctgcca ctggcgcacc
     3181 ataaacttca atgccgccgg ctggataggc accatgataa agctgttttt caaatcctcc
     3241 tcggttggga aaatcgtatt gtcgttttta aattcgtctt ccatcagctc acgcgcaggc
     3301 ttgctcgaag gcgcgtaagt aacgaaattg ccgtttttcg ccgacacttc cgggtcgagg
     3361 aagtcgttga tgtatttgtg cgcgttggcg acgtttttcg catctttcgg aatcacgaaa
     3421 gaatccaccc aaatccccac gccctctttg ggcatcatca cgcggatttt ttccttgccg
     3481 cccgcttctt cggcacggcg tttggcgatg ttcaaatcgc cgccgaaacc gattgttacg
     3541 caggtatcgc cgcgcgccaa atcatcgata aagccggacg aagtaaagcg tttgatattg
     3601 gggcggtttt tcttgagtag ggcggttgcc tccctgatgt cttccgtatt gctgctgttc
     3661 gggtttttac ccaaatagtt caacaccata ggatagattt ccgccgcgct gtccaaatag
     3721 ctgatgccgc attgcttgag tttggacgtg tattcggggt cgaacaccaa atcccactgg
     3781 ttgtccggca gcttgtccgt acccaaagcc tttttcacgc gttcggtatt gatggcgaag
     3841 gtatttgtcc cccaataaaa cggcacggcg tattcgtggc cgggatcgac cccgtccatc
     3901 agcctcatca tttcgggatt gaggtgttta taattgggaa tcagcgattt atcgattttc
     3961 tgatacgcac ctgccttaat ctgcctgccc acaaacgcat tggacggcgc gacaatgtcg
     4021 taaccggatt ttccggtcag caccttgctt tccagcgttt catcgctgtc gtacacatca
     4081 taagtaacct tgatgccgtt tttcttttca aaatcggcaa cggtttccgg atcgacgtat
     4141 tccgaccagt tgtaaatttt caatacgttt cggttttccg ccggcgccgg tttttcggca
     4201 ggcggtttgt ccgaaccgcc gcacgctgca agcagcaaag cagtcaggac ggcgagtggc
     4261 agatgtttgg ccattatcat tccttgcata tcgggttgga gaaagcggcc attatagccg
     4321 atattgggaa cagggcttca gacggcattc aaaatcccgc aatgccgtct gaaagccgcc
     4381 gcttccatag ctagaaacag ggatttgcgg taagataccg ccgttcgttt tccctgcttt
     4441 taccatgaca agacatttga gagacattga aaaaattatg aaaacctccg aactgcgcca
     4501 aaaattccta aaattttttg aaaccaaagg ccacaccgtc gtccgctctt ccagcctcgt
     4561 gccgcacgac gacccgaccc tgctgtttac caacgcgggc atgaaccagt ttaaagacgt
     4621 attcttaggt ttcgacaaac gcccgtacag ccgcgccacc accgcgcaaa aatgcgtacg
     4681 cgcaggcggc aagcacaacg acttggaaaa cgtcggctac accgcccgcc accacacctt
     4741 ctttgaaatg atgggcaact tctccttcgg cgactacttc aaacgcgacg ccatccactt
     4801 cgcttgggaa tttctgactt cccccgaatg gctcaacatc cctaaagaca aactgttggc
     4861 gaccgtttac gcggaagacg acgaagccta caacatctgg ttgaacgaaa tcggtatgcc
     4921 gtccgagcgc atcgtccgca tcggcgacaa caaaggcgcg aaatacgtgt ccgacaactt
     4981 ctggcaaatg ggcgacaccg gcccttgcgg cccctgctcc gaaattttct acgaccacgg
     5041 cgaagaaatc tggggcggca ttcccggcag ccccgaagaa gacggcgacc gctggatcga
     5101 aatttggaac tgcgtattta tgcagttcaa ccgcgacgaa caaggcaata tgaatccgct
     5161 gcccaagccg tccgtcgata ccggtatggg cttggaacgt atggccgccg tcatgcagca
     5221 tgttcacagc aactacgaaa tcgacttgtt ccaagacctg ctcaaagccg ttgcccgcga
     5281 aaccggcgca gctttcagca tggatgaacc cagcctgaaa gtcatcgccg accacatccg
     5341 ctcctgctcg ttcttgattg cagacggcgt gttgccttcc aacgaaggcc gcggctacgt
     5401 attgcgccgc atcatccgcc gcgccgtgcg ccacggttac aaactcggtc agaaacaagc
     5461 atttttctac aagctcgtgc ctgatttggt gaaagcgatg ggcgacgcgt atcccgagtt
     5521 gaaagagaag cagacgcaaa ttatggaagc cctgcgcgcg gaagaaagcc gcttcggcga
     5581 aacgctggaa aaaggcatgg gcttgttcaa ccaagtctat aatgaattga aattcaatca
     5641 aatttttgat cttctggcga aagaatttaa ggttacacct gttgaagata ttgatcttga
     5701 tatgtttagt acaagaaatt ttgtaggaag aagtgctgaa attccttata tatcaaatca
     5761 acaccctaac gtggattatc ccgctcctat ggggaacgta aatgaaaaaa tttatgctat
     5821 gccagtgaga ttaacagatg gaagaattgg tattaatatt tttcctactg atgaaactgc
     5881 acgcctaatc cttagcaatg attggaatag tttgcgcttc tattcttttt gtaattcgtt
     5941 gattacttat tatcaacaga aaggtcaatt agaaacatta aaattgaacg gcgaacacat
     6001 cttcaaactc tacgacacct acggtttccc ctacgacctg actgccgaca tggcgcgcga
     6061 actgggcatc gaactggacg aagcaggctt cgagcgcgaa atggaagccc aacgcgcacg
     6121 cgcacgcgcc gcccaaagct tcaaagccaa cgcccaactg ccttatgacg gtcaagacac
     6181 cgagtttaaa ggttatagcg aacgccaaac cgaatccaaa gtcctcgccc tctacaaaga
     6241 cggcgagcaa gtcaacgaat tgaacgaagg cgacagcggc gcagtcgtta tcgactttac
     6301 cccgttctat gcagaatccg gcggccaagt cggcgacgtc ggctatatct tctcaggcga
     6361 aaaccgcttt gaagtacgcg atacccaaaa aatcaaagcg gccgtattcg gccaattcgg
     6421 tgtacaaacc tcaggccgtc tgaaagtcgg cgacagcgtt accgccaaag tggacgacga
     6481 aatccgcaat gccaatatgc gcaaccacag cgcgacccac ctgatgcaca aagccctgcg
     6541 cgacgtattg ggcggacacg tcgaacaaaa aggctcttta gttaccgccg aatccacccg
     6601 tttcgacatt tcccatcccc aagcggtcac tgccgaagaa atcgccgaag tcgaacgccg
     6661 cgtcaacgaa gccgttttgg cgaacgttgc cgtcaatgca gccattatga gcatggaaga
     6721 cgcgcaaaaa accggcgcga tgatgctctt cggcgaaaaa tacggcgaag aagtgcgcgt
     6781 actgcaaatg ggcggtttct ctaccgaatt gtgcggcggc acacacgttt cacgcaccgg
     6841 cgacatcggc ctcttcaaaa tcatcagcga aggcggtatt gccgcaggcg tgcgccgtat
     6901 cgaagccatc accggcctga acgcactcaa atgggcgcaa gagcaagagc gtttggtgaa
     6961 agacattatt gccgaaacca aagcccaaac cgaaaaagac gtactggcaa aaatccaagc
     7021 aggcgcggca cacgccaaag cattggaaaa agaattggca cgcgccaaag ccgaactcgc
     7081 cgtccacgca ggcgccaaac tcttggacga tgcaaaagac ttgggcgcag ccaaactcgt
     7141 tgccgcccaa atcgaagccg acgcagccgc cctgcgcgaa atcgttaccg atttaaccgg
     7201 taaatccgac aacgccgtga ttcttttagc ggcagtaaac gacggcaaag tctccctgtg
     7261 cgccggcgta tccaaagccc tgactggaaa agtgaaagca ggcgacttgg tcaaattcgc
     7321 agccgaacaa gtcggcggca aaggcggcgg ccgtcctgac ttggcgcaag ccggcggcac
     7381 ggatgccgac aaattgcccg ccgtgttgga tagcgtgaaa gactgggtcg gcgcgaagct
     7441 ggtttgatgt gggaaaggca gcctgaaagg tttcaggctg ccttttgtgc aaagaggccg
     7501 tctgaaaggt ctcgtttgcc gtaggttggg tcgcgaccca acaaattttg tgaagtataa
     7561 aaatgttggt catgacccaa cctacctgcc tttttgtaca aagaggctat ctgaaaggcc
     7621 ttgtttgccg tatggtgggt cgcgacccag cagattttta ttagggtatg acccaagcta
     7681 cttgctacga taaaaaagga tttttaaatg agcattagcc ttattggact acacattacc
     7741 atagcaatca ttttgttttt tactacaaat tttatgggaa aaaaatcatc tatatttggc
     7801 tattaccaac tgtcttttag cgaagaaaat cactctccgg catttaatat tttttacaga
     7861 gcatttaccc ctatattatt tatcgttatt ttttcttggg ttgttactag tcttgaaatt
     7921 cccatttctc ttgaaaagat aaactatgta gtaatttatt attttataat tagattgtta
     7981 tctgtatttg tttttgagaa aacacacata gttaactggt ttaatcaact aacaataccc
     8041 atactatcca taacattatc atttatagta tataacaaaa tgattttgcc caaaagtttt
     8101 ctacttccat cctcacaaga agtagctact actttttgaa tagcgcttgg tggttacata
     8161 tataatatat taaataatga atcagggcat ttaaaatctt ataaagaaag aagagtaaat
     8221 tatgtaaaac acatgcacaa aaaatttgaa agttattttg gtaaaattat agataaaata
     8281 atcaaagagg atagttataa taatgatgat tttttaaccg ataagaaaaa agcactaata
     8341 tattcagttt taatttatga gaattttaat aggggactag tttatagata ttttgaaaaa
     8401 aattattttg tactggtaga ataaaaacat ttggaataat gcaagtaacc tcagcagagt
     8461 acctttccaa tgaggaaagt ataaaaaaag gcggaaatat tcttatggaa aaatacaatg
     8521 aaaaatataa tgaatctatt gatggcaata aaactctcta taaatcatat tatgaatcaa
     8581 gaagagagag tattaaaaac tacaacccag atgcaaaata cattaatgaa attgaatcaa
     8641 tttacatgat gcttggagaa atctatccaa atgcaccaga cttcatgtca ccacattttg
     8701 agggggactg ctctgagggg gaataaaatc attatttatt ctttattagt tattagcagg
     8761 atttgtcggg cataaatgcc cgacctacaa attcaatttt ttcaaacctc tgccaaatat
     8821 tttcatcttt gcaaggctgt ctgaaaaccc aaaccccatt ttcagacggc cttttttcgc
     8881 taaaatcccc ataccgttca atccgaaaac acaggagaat catcatggaa gttaccatct
     8941 ccgccatcat caatggcgaa tttgccgacc aatacggcaa gcgcggtagt cagtttaatg
     9001 aaaacgggat gctgatttaa ttctatttcc tttgaaacta ccaataacct gcctccatca
     9061 taaaactaaa agcaagccgt agcctgcatt cccacaaacc gcgtgcgttg ccatgtcaca
     9121 caccctacct gcgggcgacg caaaccttaa gagacctttg caaaattccc caaaatcccc
     9181 taaattccca ccaagacatt taggggattt ctcatgagca ccttctttca acaaaccgcc
     9241 caagccatga ttgccaaaca catcgaccgt ttcccgctat tgaagttgga ccaggtgatt
     9301 gattggcagc cgatcgagca gtacctgaac cgtcaaaaaa accgttacct tagagaccac
     9361 cgcggccgtc ccgcctatcc cctgttgtcc atgttcaaag ccgtcctgct cggacaatgg
     9421 cacagcctct ccga
//
LOCUS       DAWHRA010000080         9420 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWHRA010000080 DAWHRA010000000
VERSION     DAWHRA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.080"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..149
                     /locus_tag="WIB30_001398"
     CDS             <1..149
                     /locus_tag="WIB30_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ3797457.1"
                     /translation="AAGGKVSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELL
                     TLLG"
     gene            206..1381
                     /locus_tag="WIB30_001399"
     CDS             206..1381
                     /locus_tag="WIB30_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ3797458.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLARLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALENAGFIHYETSAFAQNNKQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQNRPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPTVFRPTEQGRLFLNDLLQCFL"
     gene            1378..1599
                     /locus_tag="WIB30_001400"
                     /pseudo
     CDS             1378..1599
                     /locus_tag="WIB30_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691731.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1878..2402
                     /gene="nspA"
                     /locus_tag="WIB30_001401"
     CDS             1878..2402
                     /gene="nspA"
                     /locus_tag="WIB30_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ3797459.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            2538..2927
                     /locus_tag="WIB30_001402"
     CDS             2538..2927
                     /locus_tag="WIB30_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ3797460.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEVRQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            3005..3835
                     /locus_tag="WIB30_001403"
     CDS             3005..3835
                     /locus_tag="WIB30_001403"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ3797461.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKHVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            3850..5307
                     /locus_tag="WIB30_001404"
     CDS             3850..5307
                     /locus_tag="WIB30_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ3797462.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMSWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILTAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(5348..5737)
                     /locus_tag="WIB30_001405"
     CDS             complement(5348..5737)
                     /locus_tag="WIB30_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797463.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(5919..6320)
                     /locus_tag="WIB30_001406"
     CDS             complement(5919..6320)
                     /locus_tag="WIB30_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797464.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(6737..7066)
                     /locus_tag="WIB30_001407"
     CDS             complement(6737..7066)
                     /locus_tag="WIB30_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797465.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVIPLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(7092..7799)
                     /locus_tag="WIB30_001408"
     CDS             complement(7092..7799)
                     /locus_tag="WIB30_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ3797466.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITF
                     KQQPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPYDKGNQRYKDASYHGKNDNSV
                     KSRAPTNGQAALDNSVQVKSTSPRRVGVDKANNEIVVLNKTQTFNNGSAEYHGHVRSW
                     QDLHTDQKNALKKAGLD"
     gene            complement(7934..8251)
                     /locus_tag="WIB30_001409"
     CDS             complement(7934..8251)
                     /locus_tag="WIB30_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797467.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(8254..>9420)
                     /gene="mafB"
                     /locus_tag="WIB30_001410"
     CDS             complement(8254..>9420)
                     /gene="mafB"
                     /locus_tag="WIB30_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ3797468.1"
                     /translation="FITDNAQRQHYEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQM
                     GNLLIQQANINGTIGYHTRFSGHGHEEHAPFDNHAADSASEEKGNVDEGFTVYRLNWE
                     GHEHHPADAYDGPKGGNYPKPTGARDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYN
                     NLGSNFSDRADEANRKMFEHNAKLDRWGNSMEFINGVAAGALNPFISAGEAVDQWMQE
                     NPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEM
                     FQTRNVDFKAKSIGTKIHDGAQGKHISGHRNYIEGKSTLNQNINPQELLNGIHSGAYP
                     VISKGARGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
BASE COUNT         2235 a         2609 c         2129 g         2447 t
ORIGIN      
        1 aagccgcagg cggcaaagtt tccggcagcg tgtccaaaaa aaccgactac gtcgtcgccg
       61 gagaagctgc gggcagcaag ctggaaaaag ccaatgcctt gggcgtttcc gtcctcagcg
      121 aggccgaact gcttaccctg ctcggctgaa cctatgccgt ctgaaccgcc ttcagacggc
      181 attatccact tttccgaacc acaccatgca caccatttcc tttccaaacc gcacccggct
      241 caccgccctg ccgcccctgt cgctttacat ccatatcccg tggtgcatca aaaaatgccc
      301 gtattgcgac ttcaattccc acagcctgaa aaacggattg cccgaagccg cctatatcga
      361 cgcgctgctg accgatttgc agcttgaatt gcccaatatt tggggcaggc cggtggaaac
      421 gatatttttc ggcggcggta cgcccagcct gtttcaggcg gaatcgattg accgtttgtt
      481 gagcggcgtg cgttcgctgt tgcgcttgca gccggaagcg gaaattactt tggaagccaa
      541 tccgggcacc tttgaaatcg agaagtttca aggatttaaa gacgcaggca tcacgcggct
      601 ttctattggc gtacaaagct tcaacgacga tatgcttgcg cggctggggc gcgtccacaa
      661 cggcaaggaa gccttaaccg ctatcgatac tgccctgaaa ttatttgata aagtcaatat
      721 cgacttgatg tacgccctgc cgaaccaaac cgttcagacg gcattagacg atgtgcaaac
      781 cgccatcgcc acgggcgcga cccacatcag cgcgtatcat ctgacgatgg aaccgaacac
      841 gccgttcggt catacaccgc ccaaaggttt gccgcaagac gaagccgccc tcgacatcga
      901 agacgcggta cacggcgcat tggaaaacgc cggctttatc cattacgaaa catcagcctt
      961 cgcacaaaac aacaaacaat gccgccacaa tttgaactac tggcagttcg gcgattattt
     1021 gggcataggc gcgggcgcgc acggcaaaat ttcctacccc gaccgcatcg agcgcaccgt
     1081 ccgccgccgc catcccaacg actacctcgc cctgatgcaa aaccggccgg gtgaagccgt
     1141 cgaacgcaaa accgttgccg ccgaagattt gccgttcgag tttatgatga acgccctgcg
     1201 cctgaccgac ggcgtacctg ccgcgatgtt gcaggagcgc acgggcgtac cgagtgccaa
     1261 aatcatggcg caaatcgaaa cggcaaggca aaaaggcctg ctggaaaccg acccgaccgt
     1321 gttccgcccg accgagcagg gacgtttgtt tttaaacgat ttattgcagt gttttttata
     1381 agcgctaaca aatacaagga ttcaaaaacg gcaaattcca acaacggaca ttctcaaaaa
     1441 tgacgcgcgg caacggcggc ggcgacaagg atggaaccgg caggcggcgg ataccggtgg
     1501 ttttcggtac ggggatgtgc agaagatgtg gagcttatcc ttgccgccgt gaaaaaagcc
     1561 ggcggcagcc gcgtcctcgc cgggtgtttc agacggcatc gccgtgcaca aaaaaccgta
     1621 tgcctgcacg cgcgcaaatt ccgtcatcca caccgtcccg cctttgctgg ttttcccaaa
     1681 ccgtttgcaa gttccgccca tccgccgcgt gatgccgccc cgtttaaagg cagggcgcgg
     1741 gacaatgcca ggtacgccgt cccgcatctt gaggcattga aaatattaca atgcaaaaag
     1801 aaaatttcag tataatacgg caggattctt taacggattc ttaaccattt ttctcccgat
     1861 cataaaggaa ctaagatatg aaaaaagcac ttgccgcact gattgccctc gccctcccgg
     1921 ccgccgcact ggcggaaggc gcatccggct tttacgtcca agccgatgcc gcacacgcca
     1981 aagcctcaag ctctttaggt tctgccaaag gcttcagccc gcgcatctcc gcaggctacc
     2041 gcatcaacga cctccgcttc gccgtcgatt acacgcgcta caaaaactat aaagccccat
     2101 ccaccgattt caaactttac agcatcggcg cgtccgccat ttatgacttc gacacccaat
     2161 cgcccgtcaa accgtatctc ggcgcgcgct tgagcctcaa ccgcgcctcc gtcgacttgg
     2221 gcggcagcga cagcttcagc caaacctcca ccggcctcgg cgtattggcg ggcgtaagct
     2281 atgccgttac cccgaatgtc gatttggatg ccggctaccg ctacaactac atcggcaaag
     2341 tcaacactgt caaaaacgtc cgttccggcg aactgtccgc cggcgtgcgc gtcaaattct
     2401 gatatgcgcc ttattctgca aaccgccgag ccttcggcgg ttttgttttc tgccaccgca
     2461 actacacaag ccggcggttt tgtacgataa tcccgaatgc tgcggcttct gccgccctat
     2521 tttttgagga atccgaaatg tccaaaacca tcatccacac cgacaaagcc cccgccgcca
     2581 tcggcgcgta cagccaagcc gtccgagcag gcgacaccgt ttacatgagc ggtcaaatcc
     2641 ccctcgatcc cgccacgatg accgtcgtcg gcaacggcga tttccgcgcc gaagtgcgcc
     2701 aagtgttcca aaacctgcaa gccgtcgccg aagcggcagg cggcacgctg gccgacatcg
     2761 tcaaactcaa cgcctacctg accgacttgg gcaattttgc cgtcttcaac gaagtcatgg
     2821 cggaatttat cgccgagccc ttccccgccc gcgccgccgt cggcgttgcc tcgctgccca
     2881 aaggcgtgca ggtcgaagcc gaagccgtcc tcgttttgaa cgcataattc cacgttcgga
     2941 ctgcatgata tttcaaaacc atgccgtccg aaccgcaaca ctttcccact tcacaacatc
     3001 tgcaatggca cattacgcaa tcggcgacat acaaggctgt ttcgacgaac tgaccgcgct
     3061 gctcggcaaa atcggtttca accacggcac ggacaccctc tggctgacgg gcgacatcgt
     3121 caaccgcggc ccgaaatccc tcgaaacgct gcaattctgc atccggcacg aaaacagcgt
     3181 gcaaatcgtc ctcggcaacc acgacctgca cctgctcgct gtcggctgcg gcgaaggcgc
     3241 gcccaaacgc agcgacacaa tcgaacccat actcaaacac cccgacggaa aaaaaatgct
     3301 cgactggctg cgtgcgcaac cgctgttgat acgcgagggc agccgcgtga tggtacacgc
     3361 cggcatcctg ccgcaatggc gcatcgccaa agccgaatcg ctcgctagag aagccgaagc
     3421 cgaactgcgc ggcaaaaaac acgtcaaatt cttctccaaa atgtacggca acaaaccggc
     3481 cgcgtgggac gaaggtttgg aaggctatgc ccgcctgcgc ttcatcgtca acgccttcac
     3541 gcggatgcgc gccctgacct ttaaaaacga actggatttc gactacaaat ccacagtgaa
     3601 aaaaatgccg ccttacctgc gcccgtggtt caaagccccc gaccggcaaa acctcgaccg
     3661 caccatcatc ttcggacact ggtcctcgct gggctacacg aatgccgaca acgtcatctc
     3721 gctggacacc ggcgcgctgt ggggcggaca gctgaccgcc gtcaacctcg aaacggaaga
     3781 aattacccaa gtccaagccg ccggcggcat agactggaaa agcttcgcaa aataacggac
     3841 cgccccacta tgcacaaaat cctgcccatc gcccacgtcc tctcccgact gggtatgctg
     3901 ttttccttta tcctgctgat acccgccgcc ctctcctacg ccttttcgga cggcgcgtac
     3961 accgccttcg ccaccaccgc gaccgttacc ctttccggct cgtgcatcgt ccggctcgcc
     4021 accctccggt tcaggcgcga actgcgcccg cgcgacggct tcaccctcgt cctgatgttg
     4081 tggctggcgt ttgccgctat ggcggcgatg ccgatgtacc tgtatttccc gaatatgggc
     4141 tttaccgacg cattttttga atcgatgtcg ggactgacca ccaccggcgc gaccgtcatc
     4201 ccccacgtcg acgggctcgc cccctccgtc aacttttggc ggcatatgct caactggctg
     4261 ggcgggatgg gcatcatcgt ccttgccgtc gccatcctgc ctatgctcgg cgtaggcggc
     4321 acgcagttat tcaaagccga aatccccggc attgacaaag aaagcaaaat gtcgccgcgc
     4381 atttcccaag tggcgaaaaa actctggttc ggctacaccc tgatcaccat cctcgcggca
     4441 gcctgcctgc attttgccgg gatgagctgg ttcgatgccg tctgtcacgc aatggcgacc
     4501 ctctcgctgg gcggattttc cacccacgat gccagcatag cttattacaa ctcccccctc
     4561 atcgaggcgg tcatcatcgt tttcaccatt gtcggcggca tcaattttgc cagccatttc
     4621 gccgccctca acagccgcag cctcaaaacc tattggaaag acgaagaatg ccggacgatg
     4681 ctgctgctgc tctccggcag catccttacc gccgccctgt acctgtggca caccggctat
     4741 tacgccggtt ttaccgaatc cctgcgctac accgccttca acttcgtctc catcggactg
     4801 gcaaacgggc tggccaacac cgacttcgca caatggccgc tcctgatttc cctgtggatg
     4861 tttttcctcg ccaacatcct cgccaactcc ggctcgaccg gcggcggcat caaaaccata
     4921 cgcgccctcg tcctgttcaa attcagcctg cgcgaaatga tggtgctgct gcaccccaaa
     4981 gccgtccgca ccgtcaaaat cagcggcaaa gccatacccg accgcctcgc gctgaccgtt
     5041 atgtccttca tcttcatcta ctttatgacc gtcgtcctct tcagcttcct gctgatggcg
     5101 agcggtatgg aatttaccac cgccttcacc gccgtcatcg cctgcatcac caacgccggc
     5161 cccggcctgg gcgaagtcgg gcccgccggc aattacgccg gtttggacgt gatgcagaaa
     5221 tggatctgcg ttaccgccat gctcttgggc aggctggaaa tcttcaccgt cttcatcctc
     5281 ttcacccccg cctactggaa gaaataaaaa atgccgtctg aaaacttttc agacggcatc
     5341 ttgtccttta aagaagcgga agctctacaa aaggaaagaa atgctgaaac ctctgataaa
     5401 tttcaggata tttctctaag gcttttaatg ctttttcttt tgaaataggc ggatcataga
     5461 catctatccc ccttaagaag gcaatgcccg ttaatgcatt tttttgatca tttgaaatgt
     5521 aaccataatt aacgactcca attacatcat aaatttcccc tatactctta tcaaaatcaa
     5581 tcaaatcaaa aatatacaat ggaattgtat caataggagt atctctgaca actatatcca
     5641 accataattt aaattcagaa agattaatag catcatagaa catacagact attgcaaatc
     5701 ctaaatcttc acaattttct ttactaattt tccacataat tatttacctt tatccattgc
     5761 ttcccaaccg atacatccgg tattttagct tctccttttc cgtcaaacac tcctttggtt
     5821 acctgctttg tgtcaaaaat accgtctgaa agccgaatat tgtttcagac ggtattttga
     5881 ttgttgggtt tttaatcaga gctttgtttg ataatttgtt attttgaata aaatacaggt
     5941 tctgtcgagc tgataacttc tattacagaa tcgctaaaaa agaaaatata agccttataa
     6001 tatttctttt caaaaaaatc aaaactttct tctttcaacc agtccatata actatcatct
     6061 attatctcga aaaaataatt tatagcagga tttctatccc aatcattttt ttctttattt
     6121 tcaaataaat attctgaaaa ttttacttta taagattcat ccgttatttg aaaatgaatt
     6181 atattgtcaa taaatatttc aatatttaat tcataatttc ggtcggatgc caaaatgttc
     6241 acatcttgtt cggtaacaca aattttgtcc acataaaatc tgtgattatt gttagaaaat
     6301 tgtaatggtt taatcttcac aatcgttttc catatctaat ttttgttttc ttattgccct
     6361 gccggataaa gcaggtctgc ctccgtactg tctatcctca catttacttt cctgccatcg
     6421 gcttttatct ccccggtttt accgtttttg ccaactttgc ggctttggcg gcggcaatgt
     6481 tgaaggcggc ttcgacggtt tcggcggcat tggggttgac ccgtactcat ccgcaaacgg
     6541 caacacgacg gacggtgttt gcttttcagc cgtctgccgt tttgaccaag gtgctgccgt
     6601 ggaaggagca agtgtaggat ttttgaagaa ctcagttctc aatatcctat ttcaaatgaa
     6661 agaaatgccg tctgaaaatt aaacacggtt tcagacggca ttttgattgt tgggttttca
     6721 acccgaccta cggttcctaa tgattttcag ataaaaataa agaaatatct tctaacgcat
     6781 cttcagccct accttgaagc tctggatcat cttgctttga atgtagtaaa ggaataactt
     6841 tatcgtaatc accgatattt gaatgtagcc tggcgagatg tccgaaacag gttagcgata
     6901 atccggcaat atccaagtct ttatttttcg ataattccat caatttctca gaaatccacg
     6961 gtacttcata tgctttggaa ttaaatacca tatcaagaat gccatctata acactttgtt
     7021 tatcatcaga agaaatcaac ttttctgttt tacttttatc agtcattttt taatttttcc
     7081 ttttgaatta actaatccaa tcctgctttt tttaaagcat ttttctgatc ggtatgcaaa
     7141 tcttgccaac ttctgacatg cccgtgatat tccgcagaac cgttattaaa agtttgagtt
     7201 ttgtttaata caacgatttc attattggct ttatcaaccc caactcttcg aggagaagtt
     7261 gatttaactt gaacggaatt atcaagagct gcttgtccgt ttgttggtgc tctacttttc
     7321 acagaattat catttttgcc atgataagaa gcgtctttat atcgttggtt gcctttatca
     7381 tacggacaat cattatgaac ccaaacccct tccgtttccg cctgactgcc cttgacgaag
     7441 taggtatgcc aatcggcgac ggtcagattg taggctttga gcggctgctg tttgaaggta
     7501 atgttttgaa ccgtctgttt tgcgccgctt tcggaaagca gggtgtcgcc ttttttcaga
     7561 cgacctgcct gtatccattt tccttgactg taaaacgggt ggattttatt ggaaatcagg
     7621 gtttggttgt tgccgatgcc gtctgaaatt tcaatgtaaa cggtttcttg atacggattg
     7681 ccgtatcggg cggtaacggg tttgtatccc gtttttccgc ttgcctcgtc cttggcgaag
     7741 acgcggtcgc cggttcggat acgggcaatg gctttgtagc cgtctgccgt tttgaccaag
     7801 gtgctgccgt ggaaggagca ggtgtaggat ttttgaagaa ctcagttctc aatatcctat
     7861 ttcaaatgaa ggaaatgccg tctgaaaatt aaacacggtt tcagaagaca ttgtgattgt
     7921 tggttttttt aacctacgct tgcgaaatta attcctttct cctgaatact gcaccattta
     7981 tttggtttaa ctccatagaa gaatgattag aataaataca ttcaaagtca gcttcatcaa
     8041 aatattctcc acacatagac cataactctg taatcaaata ttccatatct tcatggtctt
     8101 ctgtgatggc ttctcttgat aaataatttt tgatttttaa aaaccttctt ccttcctcgt
     8161 aatctacggt aattaacctg atatctgagt gtatcatgcc cagcattgct atattcatac
     8221 cgactcgcag ccagcttggt aatatattca taactattgc acctttttaa tggttttagg
     8281 gttagccgga acaatgtgaa ctccattttt acctgaatga atcgtaccaa aattggtact
     8341 taatcctgat ttcccatcgc tgcctatagg atacccaaaa tcaacaacag gatttcctct
     8401 tgctccttta gaaataactg gataagcacc tgaatgtatt ccgttcaaca attcttgagg
     8461 attaatgttt tgatttaaag tactcttacc ttcaatgtag tttctatgtc ctgaaatatg
     8521 tttcccttga gctccatcat gaattttagt cccaatagat tttgctttaa aatcaacatt
     8581 gcgggtttga aacatttctc catctacaga ctgagataat cttgaagcag tgttatgctt
     8641 gtaggagtct gagaaatccc cactaaccgc agccttcccc ggttttgccg cctttgtcag
     8701 gtttttgact ttggcaaacg gcaggacgtt gaccagggct tcgacggttt cggcggcatt
     8761 ggggttttcc tgcatccact ggtcaacggc ttcgcccgcg ctgataaagg ggttgagcgc
     8821 gccggcggcg acgccgttga taaactccat gctgttgccc cagcggtcga gcttggcatt
     8881 gtgctcgaac atttttctgt tggcttcatc ggcgcggtcg gagaaattgc tgccgaggtt
     8941 gttgtagttg tcggatatgc gttgccggat gctgcgggtg tcggtcggat tgagtttgat
     9001 actgcgggct gtgccgttga cgtgataggt gtattcgtct cgtgcgcccg taggtttggg
     9061 gtaattgccg cccttcgggc cgtcgtaggc atcggcggga tgatgttcgt gtccttccca
     9121 gttgagccgg tatacggtaa agccttcgtc aacgttgcct ttttcttcgc tcgcgctgtc
     9181 ggcggcgtgg ttgtcgaagg gggcgtgttc ttcgtgtccg tgtccggaaa agcgggtgtg
     9241 gtagccgatt gtgccgttga tgtttgcctg ttggatgagc aggttgccca tctggtgggt
     9301 atagtcttgg atgacgttga ttttgccggt gcggtcggaa acgctgccgc gcgggtcgcc
     9361 gaagaggtgg tatttgccgc cgggttcgta gtgctgccgt tgggcgttat cggtaatgaa
//
LOCUS       DAWHRA010000081         9178 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWHRA010000081 DAWHRA010000000
VERSION     DAWHRA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.081"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(209..931)
                     /gene="rmpM"
                     /locus_tag="WIB30_001411"
     CDS             complement(209..931)
                     /gene="rmpM"
                     /locus_tag="WIB30_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ3797469.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPAPAPVVVVEQAPQYVDETISLSAKTL
                     FGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYVVA
                     NNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPDRR
                     VDVKIRSIVTRQVVPAHNHHQH"
     gene            1295..2245
                     /locus_tag="WIB30_001412"
     CDS             1295..2245
                     /locus_tag="WIB30_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ3797470.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2358..3092)
                     /gene="fnr"
                     /locus_tag="WIB30_001413"
     CDS             complement(2358..3092)
                     /gene="fnr"
                     /locus_tag="WIB30_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ3797471.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3293..4714
                     /gene="hemN"
                     /locus_tag="WIB30_001414"
     CDS             3293..4714
                     /gene="hemN"
                     /locus_tag="WIB30_001414"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ3797472.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     FLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDDQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5035..6609)
                     /locus_tag="WIB30_001415"
     CDS             complement(5035..6609)
                     /locus_tag="WIB30_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ3797473.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            6934..8034
                     /locus_tag="WIB30_001416"
     CDS             6934..8034
                     /locus_tag="WIB30_001416"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ3797474.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADTRQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            8154..9095
                     /gene="mafA"
                     /locus_tag="WIB30_001417"
     CDS             8154..9095
                     /gene="mafA"
                     /locus_tag="WIB30_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ3797475.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLITPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKKPDVGNEVIRRRKGG"
BASE COUNT         2322 a         2694 c         2203 g         1959 t
ORIGIN      
        1 cggcaatggg acacaaacct gaatccgaat ccgaacctgc accccgattc tgacgatagc
       61 cgtttcagaa aaccacccaa aaaaacaaac cccgcaaaag cgggggtttg tttcacatca
      121 tcgatgccga ccgacgtttc acgaatattg caacagtacc cggtacaaaa tccgctaacc
      181 tcatgcatcg gcaagatatt gcctagcctt agtgttggtg atgattgtgt gccggcacaa
      241 cctgacgggt tacgatgctg cggattttca catccacgcg gcggtcaggt tcgatacatg
      301 caatcagagc ctcacgtttt ttggctttag agactttcgc acccagtttg gcaacttcgg
      361 cttcacaaac ttgagtcatt tgcgcttgag attcgcccaa gccgacagca gaaattctag
      421 aaacaggtac gccgttgctg accaggttgt ttgccactac gtatgcgcgg cgttcggaca
      481 gggcctgatt gtatttgtca gaacccataa agtcggtatg gccttcgacg cggacagatt
      541 ggatattggt ttgacccagg cgttgcgcca atactttcag gttgtcttga gcttcggcgc
      601 gcaatgaatc cttatcgaaa ccgaacaggg ttttggcaga cagggaaatg gtttcatcaa
      661 catattgcgg agcctgctcc acaacgacga caggcgcggg tgcgggttct ggctcgggtt
      721 cgggggcagc aaccgcatcg ccgcattcta cgcgaccttg gcttgcttta tcaaagtagg
      781 cgtttttcca gcattcgcca tagttgttgc gtacaatttc gttcgactgg ccgcttacgg
      841 tgtaaccctg aacggacgcc tcgcccgcaa cagcagtgcc ggaagcgagc aatgcaacga
      901 ataatgcgct taatttcagc tgtttggtca ttttattccc tcattaaatt tgtacagcag
      961 atacagaagc aactgctgaa ttgccaacat acaggccgct gtagaaacat ggcggcaaat
     1021 cggatatttc agtctccact ataaagaagc agggggagga ctgtcaatac tcaacgcctg
     1081 aaaacaaccg taaaacagcc tgtttcctga tagtctatca tgccgaaaaa tcaattccgt
     1141 gtcattactt tagggggatt ttttccatat cgcacgattg ccgttgcaca aaaacaacaa
     1201 acacaacagc aaagccccat accgtaccgg caagaaaata tgataaatta taaacaatgt
     1261 tcagccaccc gacacagacc cacaccgacc cgccatgaaa ttacaacaat tgaaatacgc
     1321 cttagaagtt taccggcaca acctgaacgt ttccgaagcg gccgaagcct tgtttacttc
     1381 gcaacccggc atctccaaac aaatcaaatt gctggaagaa gaaatcggca ttcagatttt
     1441 tatccgcagc ggcaagcgcg tggtttcggt ctcgcagccg ggcaaggtgg ttttggatat
     1501 tgcagaacgt attttgcgcg atgttcagaa cattaaaaat atcggcagcg agtttaccgg
     1561 acaggacagc ggttcgctga cggttgccac gacgcatact caggcacgct atgccctacc
     1621 cttgattgtt gccgattttg tgaaacgcta tccgaaagtc aacctgacca tcaaacaggg
     1681 gagccctgcc gccatcgccc aaatggttac ttcgggagag tctgatttgg caattgttac
     1741 ggaacgtata gacgaccacc ccgaactggg aagactttcc tgctacgact ggacacacgc
     1801 ggttatcgta ccgaacgacc accccttgct cgaatgcaga aaccccctcc gtattgaaga
     1861 tttggcgagg tttccgctga ttacttatga atttgcattc aatgcgggca gcagcatcgc
     1921 gcgggcattt tccaaagccc gtttggaaca gccggatgtc gcattggcgg cggcggatac
     1981 ggacgtattg aagacttatg tgcgcttggg tttgggcgtg ggactgatgg cgaaaatggc
     2041 gtacaacccg gatacggacg gcgatttgca gcttgtggat gcggcacacc tgttcgagcc
     2101 gtcgccgacg tggattgctt tgcgcagcga tacttatttg cgcggatatg cctacgactt
     2161 tatccaagcg tttgcgccgc acctgacacg cgagaaggtg gataggattc tgtacacgcc
     2221 catcagcgag gatttttcga tttaggcggc tgccggtttt cagacggcac tttgcggcag
     2281 atacaacaaa caggacagat gttttcgtct gccctgtgtt tattgagaat gccgtctgaa
     2341 atgttcgtac gggttaatca aatggcgtgc gaacaaccgg ataccatctt cttcaacacc
     2401 tgcagattga ggatcttgat gtgtttgtgt tcgacggaaa tcaatccttc ctgatgaaat
     2461 ttagataatg tgcggctgac ggtttcaagt ttcagcccga gataactgcc gatttcttcg
     2521 cgggacattc ttaagatgaa gtcgttggca gcaaaacctc gggaataaag gcgttgggaa
     2581 aggttcagca ggaaggcggc aatccgctct tcggcgcgca tattgcccaa cagcagcata
     2641 acaccttggt cgcgcacgat ttcacggctc atcatgcgga agaagtgcgt acgcaggctg
     2701 gggatgtttt gccccagttc ttcgatgtgg gtaaacggca gttcgcacac ttcgctgtct
     2761 tccaaggcga ccgcgtcgca actgtgcaca tgggaacaga tgccgtccat gccgatgagt
     2821 tcgcccgaca taaagaaacc cgttacctga tcgcggccgt cctgactggc gacggttgtt
     2881 ttgaagaagc ccgaacggat ggcaaagagc gaagtaaagg cttcgccgac acagaacagg
     2941 tattcgccct ttttcaggcg gcggctttga cggatgacgg catcgagttg gctgagctcg
     3001 ttgggcagca gcccgacagg caggcagagt tcccgcaaag aacaggaaga acacagcgtt
     3061 ttcatctgat gtgtagtatt atgcgaagcc ataccgtacc tttttgtgcg ctttgcccca
     3121 tcatgattat agtggattaa atttaaacca gtacggcgtt gcctcgcctt gtccttattt
     3181 aaatttaatc cactatatgt gcttattgac acatatcaag acaggtttat catactgtgg
     3241 cattctacca aactatccaa aatttgacta acatcataaa accacacaca ccatgaaaat
     3301 cattcagata cagaacaatc acaatgtaaa cgatgaccgc cccgagtttg accgcgcgct
     3361 gattgccagc ctgcccgcca gcggtccgcg ctacacttcc taccctaccg ccgaccgttt
     3421 ccatgacggt ttccgcgaag gcgaatatat caaagcttta catttgcgcg gtatgggcgc
     3481 gttaaacaaa ccgctttccc tttacattca cattccgttc tgcaacacca tctgctacta
     3541 ctgcggctgc aacaaaatca tcaccaaaga caaaagccgc gccgatgcct acatcgaatt
     3601 tcttgaaaaa gaaatggaac tgctcgctcc acatctgaac ggacggcacc agcttgccca
     3661 actgcacttc ggcggcggca cgccgacctt tttgagcgac gaccaaatcg aacgtgtctt
     3721 ccgcatgata cgcaaacatt tcgagttaat ccccaccggc gaatactcca tcgaaatcga
     3781 cccgcgcaaa gtcagccgcg acaccgtcct catgctcggc agactcggct tcaaccgcat
     3841 gagcatcggc attcaggatt tcgaccccaa agtgcaggcg gcggtcaacc gcatccaaag
     3901 ttacgaagaa accaaagaag tcatcgatgc ggcgcgcgaa gcggggttca aatccgtcag
     3961 cgtcgatttg atttacggcc tgccgcacca gacttcggaa agcatcaaaa ccaccatcga
     4021 taccgttttg tcgctcgatc ccgaccgcct cgccctttat cactacgccc acctgccgca
     4081 cgtgttcaaa ccgcaacgcc gcatcgatac cgccgccgtc cccgacagcg aagagaagct
     4141 cgatatgctg caatactgcg tccaaaccct aaccgaacgc ggctacgtct tcatcggcat
     4201 ggatcatttc gccaaacctg acgacgaact ctccatcgcc ctcaaagaag gcttcctcca
     4261 gcgcaacttc caaggctatt cgacctacgc ggattgcgat ttggtcgcca tcggcgtgtc
     4321 gtccatcggc aaaatcggca gcacctattc ccaaaacgaa cgcgacatcg atgcctacta
     4381 tgccgccatc gacgaaggca gactgcccat catgcgcggt tatcagctca atcaggacga
     4441 catcctgcgc cgcaacatca ttcaggattt gatgtgccgt ttcgcgctcg actatcggat
     4501 ttacgaaagt atgttcggca tcccgttcga ccgctacttc aaagacgaac tggcggattt
     4561 ggaaaaactc gccggtttgg gattggtgcg cctgaacagc cacgggctga ccgttacccc
     4621 gaaaggacgc ttcctcatcc gcaacatcgc catggtcttc gattaccacc tgcgccacaa
     4681 ggaaaccaaa gcaaaatatt cgcaaacggt gtaattgcgt ctaacgtaca aatgccatcg
     4741 gcaggataag tgttttcaag aacaggcggc ggcatatcat aacgttccgc acctttgtgt
     4801 ccgaccgttc cgaaaccaag atatagtgga ttaacaaaaa ccagtacggc gttgcctcgc
     4861 cttgccgtac tatctgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatca
     4921 ctataccaaa cggcatatcc cgacagaaca gattgcgcat aaggcacaag cccgcacatt
     4981 ccatcaacaa aatgccgtct gaacacgggt tcagacggca tcagtatttt acaatcagaa
     5041 tactgcctgt aaaaccaagt agcagacaac cgacaatacg gcggcggcag gcagggtaat
     5101 gacccacgcc aaaccgatgg gcttcatcag tttccaattg gcattacggt tgaccagacc
     5161 gatgccgagt accgcgccga ccaagatatg cgtactggac acgggcagcc ccatcagcga
     5221 cgcgcccatc acgacggatg cggcggacag ttcggcggta aaacccgagg cggggtgcat
     5281 ttcggccaaa ctcgtaccga cggttttaat cacctcttta ccgacaaacc acaaaccgac
     5341 aatcagcgcg atgccgaaag tcagcatcgc aatcgggggg acgacatttt gcgcggcaac
     5401 gctgttggta cgcaaaacat ccataatcgc ggcaaacgga ccgatggcgt tggcgatatc
     5461 gttcgcaccg tggctgaatg cgaagccgca ggcggtaaag acctgcatcc atgaaaacat
     5521 ctgaaaggtc gatttgccca agtctttacg cttgaggctt ttggcaaaaa caaacgtccc
     5581 catccacacc gccgcgccta tcataaagat ggtcaggaag ctgttgacgt tgctcatccc
     5641 caaatgcagg tttttcaagc ccttgaaaat cagcatagcg gaaatcatca tcgcgccgaa
     5701 cgaagcgata aagggaatcc aagaatgcag cgctttgtag gaatcgacat tgtttttacg
     5761 gttgtcgaac gcataaagac cgcggtaata ctccgattgc agctcttgcg gatcgaattc
     5821 gggttcgtcg taaatttgcg cgtcgtgcgc cattttggtg gcgtactcga ctttttcggc
     5881 ttcggacaaa ccctcgaaaa acaggcggtg ccgttcttta taggcctttt tttcctgctt
     5941 gatgcccttg agcgtgcctt ccgcccaagc gttgtaatct aagacgtttt tcttgacgcg
     6001 cgaaaacagg aaataggaca ccgcgccgcc caacacgggc gacaataccc aagaaacacc
     6061 aataccgccc agcttgcccc aacgtatcaa atcgcccgat gcggcatcgt tcattaccgc
     6121 catacatacc gcgctgccga caatgccgcc gataatggaa tgggtggtag ataccggaag
     6181 cccttttttc gaggcaaaca acagccacaa cgccgccgcc aaaagcgcgg acatcataat
     6241 aaacacaaac tgtatgggtt cgaaatcaac acccttcaaa tcgacgatgc ctttgcgtat
     6301 ggtattggtt acctcgccgc ccgcgatgac cgcgccgctg acctcaaata ccgccgcaat
     6361 cagcaaagcc tgcgggatgg tcagcgtacc cgcaccgacg ctggtgccga aagaattggc
     6421 aacatcgttg ccgccgatgt tgaacgccat aaacacgccg aacatcgtgg caataatgaa
     6481 taaggcggcg taattgtaat gggtataacc caagccccaa taaataaaat agccgactgt
     6541 acctaccagc atggcggcaa agacggcgtt gatggtttta acatttgcgc tcatttggat
     6601 ttgagccatg gcaatcttcc tttaatgtgg tggaaatcat gagtgttgcc gacactgtac
     6661 tgctgcctcc ggtcgaactg ccgtttcggg atatggcaga cgcatccttc cttctgaacc
     6721 gcatccgaat acccccaaac ctgaccgaat gcaaaactgc tgccggaaac ggtttgtatg
     6781 acacttctac tactggatgt tgcgggcgca ttataatatt tttccatccg tcttgaaaca
     6841 tttatttaca ctttatttac actgcggcgg caaatcggta tacgagcgtc aatacacgtt
     6901 aaaatggcgt tttgcaccag tttgggagtg atgatggaaa cacagcttta catcggcatc
     6961 atgtcgggaa ccagcatgga cggggcggat gccgtactga tacggatgga cggcggcaaa
     7021 tggctgggcg cggaagggca cgcctttacc ccctatcccg acaggctgcg ccgccaattg
     7081 ctggatttgc aggacacagg cgcagacgaa ctgcaccgca gcaggatttt gtcgcaagaa
     7141 ctcagccgcc tgtacgcgca aaccgccgcc gaactgctgt gcagtcaaaa cctcgcaccg
     7201 tccgacatta ccgccctcgg ctgccacggg caaaccgtcc gacacgcgcc ggaacacggt
     7261 tacagcatac agcttgccga tttgccgctg ctggcggaac ggacgcggat ttttaccgtc
     7321 ggcgacttcc gcagccgcga ccttgcggcc ggcggacaag gcgcgccact cgtccccgcc
     7381 tttcacgaag ccctgttccg cgacaacagg gaaacacgcg cggtactgaa catcggcggg
     7441 attgccaaca tcagcgtact cccccccgac gcacccgcct tcggcttcga cacagggccg
     7501 ggcaatatgc tgatggacgc gtggacgcag gcacactggc agcttcctta cgacaaaaac
     7561 ggtgcaaagg cggcacaagg caacatattg ccgcaactgc tcgacaggct gctcgcccac
     7621 ccgtatttcg cacgacccca ccccaaaagc acggggcgcg aactgtttgc cctaaattgg
     7681 ctcgaaacct accttgacgg cggcgaaaac cgatacgacg tattgcggac gctttcccgt
     7741 tttaccgcgc aaaccgtttt cgacgccgtc tcacacgcag cggcagatac ccgtcaaatg
     7801 tatatttgcg gcggcggcat ccgcaatcct gttttaatgg cggatttggc agaatgtttc
     7861 ggcacacgcg tttccctgca cagcaccgcc gaactgaacc tcgatcctca atgggtggag
     7921 gcagccgcat ttgcgtggtt ggcggcgtgt tggattaacc gcattcccgg cagcccgcac
     7981 aaagcaaccg gcgcatccaa accgtgtatt ctgggcgcgg gatattatta ttgagcgcaa
     8041 ataccgtctg aaagccattt ccgccgctca gacggcattt tcgccccttt tgtttacaaa
     8101 cccttaaaat ccctttacac tcaaaatccg ttcaacatca aacaaacccc gctatgaaaa
     8161 ccctgctcct cctcatcccc ctcgtcctca cagcctgcgg cacactgacc ggcatacccg
     8221 cccacggcgg cggcaaacgc tttgccgtcg aacaagaact cgtcgccgca tcgtcccgcg
     8281 ccgccgtcaa agaaatggat ttgtccgccc taaaaggacg caaagccgcc ctttacgtct
     8341 ccgttatggg cgaccaaggt tcgggcaaca taagcggcgg acgctactct atcgacgcac
     8401 tgatacgcgg cggctaccac aacaaccccg aaagtgccac ccaatacagc taccccgcct
     8461 acgacactac cgccaccacc aaatccgacg cgctctccag cgtaaccact tccacatcgc
     8521 ttttgaacgc ccccgccgcc gccctgacga aaaacagcgg acgcaaaggc gaacgctccg
     8581 ccggactgtc cgtcaacggc acgggcgact accgcaacga aaccctgctc gccaaccccc
     8641 gcgacgtttc cttcctgacc aacctcatcc aaaccgtctt ctacctgcgc ggcatcgaag
     8701 tcgtaccgcc cgaatacgcc gacaccgacg tattcgtaac cgtcgacgta ttcggcaccg
     8761 tccgcagccg taccgaactg cacctctaca acgccgaaac ccttaaagcc caaaccaagc
     8821 tcgaatattt cgccgttgac cgcgacagcc ggaaactgct gattacccct aaaaccgccg
     8881 cctacgaatc ccaataccaa gaacaatacg ccctttggac cggcccttac aaagtcagca
     8941 aaaccgtcaa agcctcagac cgcctgatgg tcgatttctc cgacattacc ccctacggcg
     9001 acacaaccgc ccaaaaccgt cccgacttca aacaaaacaa cggtaaaaaa cccgatgtcg
     9061 gcaacgaagt catccgccgc cgcaaaggag gataaaccgt gaaaccgctg cgcagactga
     9121 caaacctcct tgccgcctgc gccgtagcgg cggccgcact catacagccc gccctcgc
//
LOCUS       DAWHRA010000082         9080 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWHRA010000082 DAWHRA010000000
VERSION     DAWHRA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.082"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(190..942)
                     /locus_tag="WIB30_001418"
     CDS             complement(190..942)
                     /locus_tag="WIB30_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ3797476.1"
                     /translation="MITLPSLNNLPSKWDEIRHWLESRVFECGVMPHPNLTECEAKQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGSRGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALEHGEVQTALF"
     gene            1292..1815
                     /locus_tag="WIB30_001419"
                     /pseudo
     CDS             1292..1815
                     /locus_tag="WIB30_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            complement(1904..2183)
                     /locus_tag="WIB30_001420"
                     /pseudo
     CDS             complement(1904..2183)
                     /locus_tag="WIB30_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(2555..3700)
                     /gene="dapE"
                     /locus_tag="WIB30_001421"
     CDS             complement(2555..3700)
                     /gene="dapE"
                     /locus_tag="WIB30_001421"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ3797477.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLVERLYKIGFAAEEL
                     HFGDTKNIWLRRGTKVPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(3814..4434)
                     /locus_tag="WIB30_001422"
     CDS             complement(3814..4434)
                     /locus_tag="WIB30_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ3797478.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(4468..4977)
                     /locus_tag="WIB30_001423"
     CDS             complement(4468..4977)
                     /locus_tag="WIB30_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ3797479.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(5072..5623)
                     /gene="azu"
                     /locus_tag="WIB30_001424"
     CDS             complement(5072..5623)
                     /gene="azu"
                     /locus_tag="WIB30_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ3797480.1"
                     /translation="MKTYLALISAAVIGLAACSQEPAAPAAEATHAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(5904..6326)
                     /locus_tag="WIB30_001425"
     CDS             complement(5904..6326)
                     /locus_tag="WIB30_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ3797481.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLILLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESLDSAAAKLEKRHPELAEALANLVRRHGA"
     gene            6457..>9080
                     /gene="secA"
                     /locus_tag="WIB30_001426"
     CDS             6457..>9080
                     /gene="secA"
                     /locus_tag="WIB30_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ3797482.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADEALSDEEKQAQISALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLMDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEA"
BASE COUNT         2201 a         2584 c         2251 g         2044 t
ORIGIN      
        1 ggggcgtatt atagcgtccg gaaaaaatgt gtcaatcctt aattttggaa aaatgggcga
       61 caaaacgaca agcatatgaa tcagaaagac attaagaccg atgccttaaa aggattgccg
      121 ttgtatgaat ttccacagcc gtcatcacac catatttaag cccgatgagc cgttctgccc
      181 tccccccgct taaaacaatg ccgtctgaac ttcgccgtgt tccaaagcca gtaaaaactg
      241 tttgcggttc aacccgcccg cgtagccggt cagtttgccg tcgctgccga tgacgcggtg
      301 gcagggaatc aggatagata ctttgttctg cccgttggcg gcggcaacgg cgcggacggc
      361 tttggggttg cccaaacgct gcgcctgctc cttgtagctg cgcgtttcgc cgtaaggaat
      421 cgccaagagc gcgtcccatg cctgcttttg aaactcggtg ccaatctgct ccaaaggcgt
      481 ggcaaaggtt ttcagacgac ccttgaagta taagtccaat tcctgccgca aaagttgcgt
      541 ccgctcatcc tcccgaaaca caaaccgtcc gcgcaaggct ttttggacgg cggcaatttc
      601 ctgttccaaa tgcttctgtc cgacaaattc cagcaaacac aaacccctgc taccgaacac
      661 cgccagcatc tcgcccaaag gcgtggcaat ggcggcacac accagctcgt tcaaactgtc
      721 gggataacgc gcttccaaca gacggatggc gcggcggatg cggacatatt cttcaggcgc
      781 gcagccgata ttgtcccaaa aatcccgctc gaactgtttg gcttcgcatt ccgtcagatt
      841 gggatgcggc ataacgccgc actcaaaaac ccgagattcg agccaatggc ggatttcatc
      901 ccattttgac ggcagattgt ttaaggaagg cagggtaatc atttgtttgc tccgtatccc
      961 tatcatagat ttgacggcaa aatccccaat ttttgccatt cccgcacgcc ggagcaggaa
     1021 cgggctatga cgtaaatctt gagggttagg ttgcggcaat acctaaatat tcgatatttc
     1081 taaagcatca gagaaaggaa tgtttcaaca cacaggacga cacataaagc gccgccccat
     1141 gaaaaatttc agacgacctg caaagggtcg tctgaaacca cgatttttgc atttgcgcat
     1201 tctggcacat catccaaccg tttcggcaca ttcctgccgc cgttgacagc ctataatgaa
     1261 tccacttatt catcaagcaa aggaatcatc tatgcaaacc ctcatcctct ccgccgtact
     1321 gctggctttt tcaaccgctg cctttgccgg gggcgcattc acgctgcaat tcgacaaccc
     1381 gtccgaagac ggcggcttca cgcaaaacca gcttttgagc gcgccttacg gcttttgctg
     1441 ttcaggcgac aatgcttcgc ccgcgctgtc gtggaaaaat ccgcccgccg ggacaaaagt
     1501 ttcgtcctga ccgtttacga taaagacgcg ccgaccggac tgggctggat gcactgggtg
     1561 gtcgccgaca ttcccgccga tgtccgccgc cgcaatgcgg cctcgctgca attaagccgc
     1621 tgcgccagca tcgccgacga cccgtccgca gccatatcgg cggtaatcag tttgcggatt
     1681 tgccgcatca ggttgacgcc ttcgtacacg gcaaaaccga tgccgtcatg ctgcaaccac
     1741 gccaacacgc cgcaaagcgc ggcctccgcc gcattgtgcg gcacttcttc atccgtcagc
     1801 accgccgccg cataatcaaa cgccgcgccc atccgcccgg ggtgcggcag cttcaccgca
     1861 tcgcacactg cctgcgccgt cccgtattgt gcggcgaacc tttctacggt ttcctgttcg
     1921 aaagcaatcc attgcgcctg atagaggtcg tctgaatcag gaatattgat gacgtcaaac
     1981 gtctgcccgc ctgccaacgc gacaacctca cccgccgcaa tcctcaattc gcgccccgcc
     2041 caccgcaact tcttacatcc gcgccgcacg ataagcacag ctggttcata taccgccacg
     2101 ctgcggtaca aggcggtatg atgttgcacg atgccgccca aagcacccaa tcgttcgcgc
     2161 gtatgaaagt aatttgacgg cactattatc tccaacacgc agggccgcac ataaggcgca
     2221 gccctgttta ccctgatttg gaaggggtta cttcccaaaa ataaaccaaa atcccactgc
     2281 cccgaatggc cgggtttcaa ccatattccg tcggtgtgcc tgcaacttga ctccctcccc
     2341 tgtgggggag ggttggggag agggtaaaac ggggcagata cagacaatat ttccgttacc
     2401 gccccgatgc cctctcccta accctctccc acgggagagg gaatggattg ccgttgaaat
     2461 aaatcgctct acataaaaaa tcaatgtgtt atctcaaacc cacgttaggt ctaagcaaat
     2521 ggtcggacat ccatatccgg caagcaagct gctttcagac ggcatttcca gccaacaacc
     2581 gcgccaatat cccctcatac accgcagaca gcttcggaat gtcgtttagc cgcacgtttt
     2641 cgttgatttg gtggatggtc gcattggacg ggcctaattc gatgagttct tttgcaatgg
     2701 ctttgatgaa gcgtccgtcc gaagtgccgc cggtggtgga caattcggtc tctacgccgc
     2761 agatttcggc aatggcggcg cgtgccacgt cggtcagttt gcccgcgtgg gtcaggaagg
     2821 gctgccccga acacgaccac tgcaaatcgt attgcacacc gtgtttgtcc aaaatggcgt
     2881 ggacgcgttg tttcagccct gcttcggtgg actcggtgga gaagcggaaa ttgaatttga
     2941 cgctcagctc gcccggaatg acgttggtcg cgcctgtgcc gccgttgata ttggaaattt
     3001 gaaagctggt tggcgggaaa tattcgttgc cttcatccca gacttcctgc gtcagctcta
     3061 acaaggccgg ggcaaaagta tggacgggat tgattgccaa atgcggatag gcaatatgac
     3121 cttgcttgcc tttgacggtc aggttgcccg acagcgagcc acgccggccg tttttaatca
     3181 tatcgcccaa tttgtccacg gcggtcggct cgccgacgat gcagtagtca atcaactcgc
     3241 cgcgcgcttt caatacatcg acgactttgg tcgtgccgtc caacgcgtcg ccctcttcgt
     3301 cggaagtaat cagaagcgca atgctgcctt ggtggtcggg atgttcggca acgaagcgtt
     3361 cacaggcggt aacgaagcag gcgatgctgg ttttcatgtc cgccgcgccg cgcccgtata
     3421 atcttccgtc gcgttcggtc ggctcgaacg ggggcgaatc ccatttttca acagggcctg
     3481 tcggtacaac gtcggtatgc cctgcaaaac agacgacggg aactttcgtg ccgcgtcgca
     3541 accagatgtt tttggtgtcg ccgaaatgga gttcttcagc cgcaaaaccg attttgtaca
     3601 ggcgttcgac aagcagtttt tggcaatctc ggtcgtcggg ggtaacggat gggcgggaaa
     3661 tcaacgcttt ggctagttct agggattggg tttcggtcat atttgttcac ttttgaaatt
     3721 agaccatctg aaacgttctg aatgtgattt tcagacggca tttaggttag gttggcatac
     3781 ggggtgggta tttttacccg tcagtcttct gaatcatttg ccgtgacacg cttcgtaaag
     3841 aggcagcaaa tcttccaccg tttccgctat ccatttcgcg acatcctgcc tgcccaaatc
     3901 gtcgcgttcg atgtgtttgc cgatgcagaa aaagtcttcg tcgttttgca actttcggtc
     3961 ggactcgttt tgttgcgcga cggtgcggta gtcgtcgtat tcgctttccg caccgtgcca
     4021 catatcgaaa gaagcgtatt tttcggtatc aaaattatcc aaccagcggt tgtattcggg
     4081 cagcgcaatc ggcgacacgt cggctttgta gcagtgccaa tccaagctga cgctcaggcg
     4141 gcggcggttc aataaaatcg ataaaatcgc ggcggaattt ttatattgtt cgtatttgaa
     4201 gtaggcaaag aaatgggcgc gaacctgcca gccgttacac cagcgttcga tgtgcggcgg
     4261 cgcaaacggc gcacccaatt cggcggcaac ctgctgaatc agctgctgcc atatctgcca
     4321 gttttcttta tagtcagcct tgatttgcgg aatgctttca ggctggtatt ttttaagctg
     4381 ggaaaattgg aaaaacggga tattgaacaa atcgcaactt ttcggggtca gcataatata
     4441 tccttgagac gattgtttca gacggcatta tttgcgccgg cgcgccgcca taatttcgcc
     4501 gatttcggtc agtttttctt ttgggataaa ggtgttgccc atatcaaaca gcggctcttc
     4561 aatcgccaaa tgaacatcat atcccgccac aaaacgtttg aacgcttcct catcggggac
     4621 ataagcgttg tctgcttcga gtttggcaaa ttcggcggaa acagccgccc agttgtcgtg
     4681 cagcccgata tgttggcgca aaagctcgtc cacgctttct tgggcttgcg gcgcatattg
     4741 cagcagcagc gggaagaagt tttcttcttc gtcttcatgg tgcagcggcg cggcaacgtt
     4801 gaaatactgg gcgatttggc ggatggtttg caaaacaatc tgattgcagc cgttttcggc
     4861 gatatagtcc gacagcatgg cgacttgtcc gcaaaaacgg cgcactttgc cgtggcaggc
     4921 atacagcatt tcaatcggtt cggcaaaggt aacgcttttg gtttcaaacg gattcatgtt
     4981 ttcgttctca acgggcactt ttcaagcagt cattttataa taaaacagcc tgcacaaagc
     5041 aggctgtccg tcttttgaga ctttaagcgg attaatcgac caaagtcact ttgccgttca
     5101 tcaaagcacc gtgacctggg aaggtacaag cgaatttgta ttcgccgtca gccagtttgg
     5161 caggatccaa agtcagggaa gcttcttcgc cgccgccgat cagtttggtg tgggcaacaa
     5221 cgcgcgcatc gtcaggtttg acatagtcgg tatcggcagc acctacgccg tctttaaata
     5281 cgccgtccat gtcttcagct ttggcaatca caaggttgtg acccatgctg gctttgggtt
     5341 gcgtaccggt atgtttcaga gtgatggtga actctttaca tgctttgctg acttggatgt
     5401 ctttggtgtt gaactgcata ttgtcgttgg attcgacagt tgccgcacag ttgccggcag
     5461 caggggcttc ggcagcatct gcaggagcag cttcggcggc aggtgcttcg gaagcgggtg
     5521 cttcagcagc atgagttgcc tcggcagcag gcgcggcagg ttcttgagag caggcagcca
     5581 aaccgataac ggcagcagaa atcagagcca gatacgtttt cataacaaat ctccaatcga
     5641 taaaataata ttcggtttta cggaaatcaa agtgcaaccg ccattaacaa aaccttgaaa
     5701 aagattccgc cgcgttgcac aaacagatgt ttcggggcgg cattttgcta caaatttcat
     5761 ttgaaatcaa agcctgtctg caagtttaca atcgtttact ttaaaagggg caattttacc
     5821 ccgaacctat ttctttagta ttagacccat tatcctttac ttcttaatat taacggatgt
     5881 ttacacaaat tcccgtatac attttatgcg ccatgccttc tgaccaagtt tgccaatgcc
     5941 tccgccaatt cgggatgccg tttttccaac tttgccgccg ccgaatccaa actctccagc
     6001 gcagccttac tcaaatgcag ggtattggtt ttcggcggtt tttccggttt cgggaccagc
     6061 ctgaccgaaa cagagcgtat cgaagcatca agccctgcca actgcggcaa taccgacggg
     6121 gcaatcatct tcaaacgcga tgccgccata ttgtttgccg ccaaaagaat cagcctaccg
     6181 tcttcgatac atgccgtctg aaaatgcggg tgcaggttgg caggcagcag ttttttcacg
     6241 gcggcatcca accgccgcca ctgtcccgcc tgtttcaaaa gtccggaaag cagcgcgtcc
     6301 cgcctgccca actgttccaa attcataaaa catacaccca aaaagattga aataccgcaa
     6361 acgcgccttt atttcagacg gcattagcac tttgcacaaa cgcttgtgtt aaaatcgcgt
     6421 tttcgcccac tattatatca ggcgcaggaa ttattcatgc tgacaaacat tgccaagaaa
     6481 atcttcggca gccgcaacga ccgcttgctg aaacaatacc gtaaatccgt tgccagaatc
     6541 aacgcgctcg aagaacagat gcaagcccta agcgatgctg atctgcaagc caaaactgcc
     6601 gaattcaaac aacgcctcgc cgacggtcag actttggacg gcattttgcc cgaagccttc
     6661 gccgtctgcc gcgaagcgtc ccgccgcacc ctcggtatgc gccacttcga cgtgcagctt
     6721 atcggcggta tggtgctgca cgacggcaaa atcgccgaaa tgcgtaccgg cgaaggcaaa
     6781 accttggtcg ccaccctcgc cgtctatctc aacgcgctgg ccggcaaagg cgtacacgtc
     6841 gttaccgtca acgactacct cgcctcacgc gatgcgggca ttatggagcc gctctacaat
     6901 ttcctcggcc ttaccgtggg cgtgattatt tcagatatgc agccgttcga ccgtcaaaac
     6961 gcctatgccg ccgatatcac ctacggtacg aacaacgaat tcggcttcga ctacctgcgc
     7021 gacaatatgg ttaccgacca atacgacaaa gtgcagcgcg aattgaattt tgccgttgtc
     7081 gatgaagtgg attccatctt gattgacgaa gcgcgcactc cgctgattat ctccggccag
     7141 gcagacgaca acatccagtt gtaccaaatc atgaacaccg ttccgcccca cctcgtccgc
     7201 caagagacag aagaaggcga aggcgactac tgggtcgatg aaaaggcgca ccaggtcatc
     7261 ctgagcgaag caggtcacga acacgccgag caaatcctga cccaaatggg attgctggca
     7321 gaaaacgact ccctctattc cgccgccaat atcgccctga tgcaccacct tatggcggca
     7381 ttgcgcgcgc attccctctt ccacaaagac caacattacg tcatccaaga cggcgaaatc
     7441 gtcatcgtgg acgaattcac cggccggctg atgtccggcc gccgctggtc ggagggtctg
     7501 catcaagccg tcgaagccaa agaaggcgtg gaaatcaaac gcgaaaacca aacccttgcc
     7561 tccatcacct tccaaaacta tttccgcctg tacaccaagc tctccggcat gaccggcaca
     7621 gccgataccg aagctttcga gttccaaagc atctacaacc tcgaaaccgt catcatcccg
     7681 accaaccgcc ctgtacagcg caaagacttc aacgaccaga ttttccgttc cgccgaagaa
     7741 aaattcgaag ccgtcgtcaa agacatcgaa gaatgccaca aacgcgggca gcccgtcctc
     7801 gtcggtacga ccagcatcga aaactccgaa ctggtctccc accttctgca aaaagccgga
     7861 ctgccacaca acgtcctcaa cgccaaagaa cacgaacgcg aagcgctgat tgtcgcccag
     7921 gcaggcaaag tcggcgcgat taccgttgcc accaatatgg cgggacgcgg tacggacatc
     7981 gttttaggcg gcaacctgaa gcaccaaacc gatgccatcc gtgccgacga agccttgagc
     8041 gacgaagaga aacaggcaca aatctccgca ctcgaagacg gctggcaggc agaacacgac
     8101 aaagtgatgg aagcaggcgg tttgcacatc atcggtacgg aacgccacga aagccgccgt
     8161 atcgacaacc agttgcgcgg acgttccggc cgtcagggcg accccggatc cagccgtttc
     8221 tacctctcgt ttgaagaccc gctgctgcgc ctattcgcac tcgaccgcgc cgccgccatc
     8281 ctcaaccgcc tcgcccccga acgcggcgtt gccatcgagc acaacctgct gacgcgccaa
     8341 atcgaagggg cgcaacgcaa agtcgaaggc agaaacttcg atatgcgcaa acaggttttg
     8401 gaatacgacg acgttgccaa cgagcaacgt aaagtcattt acagccagcg caacgaaatt
     8461 ctgaccagca aagacatcag cgacctgatg aaggaaatcc gttctgatgt cgtcagcgac
     8521 ctcatggata cctatatgcc gcccgacagc atggaagaac aatgggacat cccgactttg
     8581 gagaaccgtc tggctgccga attcagactg cacgaagaca tccaatcctg gctgaaggcg
     8641 gacaatgcga ttgacggtca agacatcaaa gaacgcctga tcgaacgcat cgaaaacgaa
     8701 tatgccgcca aaaccgaact ggtcggtaag caggcaatgg ccgatttcga gcgcaacgtg
     8761 atgttgcagg tcatcgacaa ccaatggcgc gaacacctcg ccgctatgga ctacctgcga
     8821 caaggcatac acctgcgcag ctatgcccaa aaaaatccga agcaggaata taaacgtgaa
     8881 gcctttacca tgttccaaga cctgtggaac ggcatcaaat tccatattgc ctccctgctt
     8941 acctcggttc aaatcgaaca aaaccctgtc gcggtggttg aagagcaacc catcggcaac
     9001 atccagtcca tccattccga atcgcccgat atggaagaac ttttgggtca gtcgcaaacc
     9061 gatttggtta ccgaggcctt
//
LOCUS       DAWHRA010000083         9043 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWHRA010000083 DAWHRA010000000
VERSION     DAWHRA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.083"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(17..532)
                     /locus_tag="WIB30_001427"
     CDS             complement(17..532)
                     /locus_tag="WIB30_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ3797483.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(608..1027)
                     /locus_tag="WIB30_001428"
     CDS             complement(608..1027)
                     /locus_tag="WIB30_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797484.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(1148..2146)
                     /locus_tag="WIB30_001429"
     CDS             complement(1148..2146)
                     /locus_tag="WIB30_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ3797485.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGYTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLREEGGEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWAAFKQLAVSLAK"
     gene            complement(2148..2627)
                     /locus_tag="WIB30_001430"
     CDS             complement(2148..2627)
                     /locus_tag="WIB30_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797486.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(2679..3458)
                     /gene="pgeF"
                     /locus_tag="WIB30_001431"
     CDS             complement(2679..3458)
                     /gene="pgeF"
                     /locus_tag="WIB30_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ3797487.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPNADAS
                     VDDTGKAACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            3633..3746
                     /locus_tag="WIB30_001432"
                     /pseudo
     CDS             3633..3746
                     /locus_tag="WIB30_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4025..4972
                     /locus_tag="WIB30_001433"
     CDS             4025..4972
                     /locus_tag="WIB30_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ3797488.1"
                     /translation="MNILSKISSFIGKTFSLWVALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAG
                     SMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(5124..6248)
                     /gene="rluD"
                     /locus_tag="WIB30_001434"
     CDS             complement(5124..6248)
                     /gene="rluD"
                     /locus_tag="WIB30_001434"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ3797489.1"
                     /translation="MQNTSFDNESDYSDDSDFVSAPETENCICLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            6247..7050
                     /locus_tag="WIB30_001435"
     CDS             6247..7050
                     /locus_tag="WIB30_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ3797490.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(7108..>9043)
                     /locus_tag="WIB30_001436"
     CDS             complement(7108..>9043)
                     /locus_tag="WIB30_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ3797491.1"
                     /translation="VQFAAKAVDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVG
                     ELNLRGLNREAWALSNGIGGAGTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGG
                     LSDGIGLMRALSVGEQSALRPELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLL
                     ACSPRLPARPRAWVLAAGCAGALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWA
                     TWWQALAAVLLFDPSAVLGVGTWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVL
                     SLVLLGYLFASLPLISPLVNAVAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTL
                     RFLVWLADVSPEFAVASAPLPLLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSP
                     GVPENEVAVTVWDAGQGLSVSVQTANHHLLFDTGTAAAAQMGIVPSLNASGIRYLDYL
                     VLSHHDSDHDGGFQAVGKIPNGGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSER
                     KNIDDNGKSCVLRVVADGAALLVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTS
                     SSGVFLNAVSPEYAVASSGYANAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRG
                     GVKAQRLRGYKFYWQKKPFE"
BASE COUNT         2049 a         2702 c         2323 g         1969 t
ORIGIN      
        1 tttgcggtat aaaggcttaa ggctttcctt tgaccgccac caatcttgtt cctgcaagaa
       61 aatcatacag aaactgccga tcgggattca gcagtgcgaa cccccacggt aaaatcagcc
      121 aaatcaatgc cgcgccggcc gcgcccttgg gcggaatgcc gaggaagtgg cgtaatccgg
      181 cataagcaag cataggaatg aagacgataa atatgcacgc ccaaataaag cgcaggcgca
      241 gcaggtgcaa agacggctgt atgccgttaa ggtcgcacaa gccgattttc catgtcctca
      301 tcgccaaggt ctgcccctga ccgtgccagt tggcgcgaaa ataaagccac catgatccca
      361 ttatcaggat actcgttacc aatgcagaaa ccgcgataga aacgggattc agaaaaatgg
      421 cggcaatacc ggcaaacaat gctgccaaac aggttgccgc accgaccagc agcatttcgt
      481 acagcatggc ggcaaagcgg cgtttgagcg gggcggtttt cagtgtcatc atcttttcaa
      541 tcagaatatg ccgtctgaaa cgccaacagg cttcagacgg catttatgca aagatgcctt
      601 gcggcggtta acggtgcgga cgtttctgac cgcgcgactg ttttgccgcc atttgattga
      661 tttcctgacg tgtcgggcgt tttttgaaca tagcgtgcgc ctcgtccacc gaacattttt
      721 gcgacaggca gattagggta atcgcatctt tctggtgttg tccgccgctg tggtacatac
      781 ggcgcaggtt tgcctcgttg attctgcctt tttcgggatc gatgtcgaat tttttcagac
      841 gttcgataaa ctggcgttgc tgtcggcggg atgtccaaac aaagccgccc gcagcggcgg
      901 cgacgatgac ggcggcaagc aggatggcaa gcggccagga gtcgacaatc aatacgataa
      961 tgaggcttaa aacggcggtg gtcagtaaaa tcaggccgcc caagcgcagt ttggtcttat
     1021 tgtccatagg gttaatgttc ctttattgag gtggtcgtgc cgtctgaaat gttttcggac
     1081 ggattggtga atacttttca ggattaacgg tattttacag tattttcgta ttttggggat
     1141 tgccgcttta tttcgccagc gacacggcga gttgtttgaa cgccgcccac gcatcgcctt
     1201 cttccgcacc tttgatgatt cggtcgattt gggcgcaggt tttgagcgcg tcaagcaggc
     1261 ggacgacgga aatccgcttg accgcaagcg gcgcgagcgt ctgcttgtcg ccccaaagtc
     1321 tgagactgtt gcggacggat tggatgctct gcccctgctt cagggcggcg gcaagccgga
     1381 tcagcgtccg cacgtcttcg gcaaccgccc acagaagcag caccggttcc ccgccttctt
     1441 cccgcaatcc gtccaagagc ctgcatacgc gcgggacatc gcccttcatc cacgcgcctg
     1501 ccagttggaa cgcgtcgaag cgggcgacgt tggcaacggc ggtttgcgcc tcatcgatat
     1561 tgacggtata ccctttcgga tacagcagcg cgagcttgtc gatttcctga cgcgccgcca
     1621 acaaattgcc ttccacgcgc tcggcaaaca gtgccaatgc gtctgcctcg atacccaaac
     1681 cgattttgtc cagccgtccg cgtatccatt ggggcaaggc cgccgcgccg accggtttgg
     1741 cttcccacac ttcccccttt gccgccaatg ctgcaaacca tttggactgg agctgggttt
     1801 tctccagttt gggcagtaaa accagcgtta ccgtatcttc cggcaggcgg gcggcaaaat
     1861 cctgcaacgc ctcgccgccg gttttgccgg gttttccatt ggggatatgc agttccaaca
     1921 gcttcaaatc ggcaaacaga cccgcactgc ctgcggtttg cagcagttcg ttccaatcga
     1981 aggcattgtc tgccgtataa acttcccgat tgaggtaacc ttgtttcttc gccgccgccc
     2041 tcaatgcgtc caatgcctcg atacgcaaca gttcttcctc gccgtggatg acgtacaggg
     2101 gtttcaaagg cgcgtccgta tcaatgtgtc cgatatgtgc cgccacgtca ttccgccttc
     2161 agaaaggtca ggcggcggac aatctgttcg gcggcatcct gccgcatttc cgcccacaat
     2221 gccgcttcct cttcctgttt gcccaagatc tcgttgtcgg cataagcaag gacgcggcgg
     2281 acggacacgg tcatcggttt gccgaccggc tcgccgcgtt tcaatacctg cgcttcaacc
     2341 gtcaagatta aaagatattc gttgataact gccgcacggg taacggtgta ggtttccttg
     2401 ttttgggaaa cgctgtctat acgcagggtc atctgcgcgc cggcagcatc gtccaccctg
     2461 cccgaagcct gatacagcgc ggtttccaaa ggaaactgca atgcctgtcc gccttcgatg
     2521 tgccagctcc ggtaggtcag cggcggagaa atgccgtctg cacctttcag gtggaaaccg
     2581 cacgcgccca gcatcagcac tgcggcagta agaaacagtt tgttcatggc ttgcctttct
     2641 caaaagcaca aagtcggtag attatatcag cggcgtgttc agacggcatt gccgtccagc
     2701 caaatcaggc tcgccatacg ccctgtcgct ccgtcgcggc ggtaggaaaa gaaagtgtcc
     2761 cgttccaaaa ccgtacaatg cgtgccgcca tatacgccgc ccacgccttc acgcttcaga
     2821 atcaggcggg cgagcgcgta aaggtcggca aggaatttgc cgccgcctat gccttcaaat
     2881 gcggcggcgg cttcgggcat gggcgtgcag aacgcatcaa acacatcctg tccgacttca
     2941 aacgcatccg cactgatggc ggggccgaga tacgccatca tttcgacagg cggaaccttc
     3001 atcgcagcaa tggtgttttg caatacgccg cccgccaaac cgcgccagcc cgcgtgtgcg
     3061 gcggcaaccg ccgtacccgc cctgtcgcaa aatagaacgg gcagacagtc tgcggtcatc
     3121 acggcacagg cggccttgcc cgtgtcgtcc acggaagcgt ccgcattggg tgtgcctccc
     3181 aacgcttcgg cagcattgac gacgacggtg ctgtggattt gattgaggta ggcaacgggc
     3241 aatccgacct gctgttgcac gatttcgcgg ttacggcgca cggcttcagg gtcgtcgccg
     3301 acgtgcgtac cgaggttcaa actctgatac gcaccttggc tcacgccgcc gttgcgcgtg
     3361 gtaatcaggg ttttcacatt ggcgggcgcg ggccaatcgg cggtcaggaa gtttttgcct
     3421 ttcggggcga gatttagggt ttctgtgatg gttttcattg gatattggaa taggtgtgtg
     3481 gcggaaaggg ttgattaact gccttcgggg caaatggatg tttgtttttt cgtcattccc
     3541 gcacaggcgg gaattcggag attcgggatt gttttcaatt gttcaaacgt tcggattcgg
     3601 attcttgcag gtgcgggaat gatgccctta tatatagtgg attaaattta aatcaggaca
     3661 aggcgacgaa gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ccgtactggt
     3721 ttaaatttaa tccactatac tttctccgag ctttatatgt ttcaacagag gatggcacat
     3781 caagcaccgc actgcgtgtt gcccgaacga gactaccctc cgattagatt ctatcgctat
     3841 aaacagacga gtttcaaccg aaaaggaatg gggataaagt ccatttccga cacctctcgg
     3901 gcgatgccgt ctgaaaacca atctccactt tcagacggca ttgtttaggc acggtcattt
     3961 tgcaaattaa ggttaaggcg tgtatgcttg ccttccctta aaaacaaaca cgcaacgcat
     4021 tattatgaat atcctcagta aaatcagcag ctttatcgga aaaacatttt ccctctgggt
     4081 cgcgctcttt gccgccgccg cttttttcgc gcccgacacc ttcaaatggg cggggcctta
     4141 tattccttgg ctgttgggca ttattatgtt cggtatgggt ttgacgctca aaccttccga
     4201 cttcgatatt ttgttcaaac atcccaaagc cgtcatcatc ggcgtaatcg cacaattcgc
     4261 cattatgccg gcaaccgcct ggctgctgtc caaactgttg aacctgcctg ccgaaatcgc
     4321 ggtcggcgtg attttggtcg gctgctgccc gggcggtacg gcttccaatg tgatgactta
     4381 tctggcgcgc ggcaatgtgg ctttgtcggt tgccgttacg tctgtttcca ccctgatttc
     4441 cccattgctg actcccgcca tcttcctgat gcttgccggc gaaatgctgg aaatccaagc
     4501 gggcagtatg ttgatgtcca tcgtcaaaat ggttttgctc cccattgttt taggtttgat
     4561 tgtccataag gttttgggca gtaaaaccga aaagctgacc gatgcgctgc cgctggtttc
     4621 cgttgccgcc atcgtgctga ttatcggcgc ggttgttggg gcaagcaaag gcaagattat
     4681 ggaaagcggc ctgctgattt ttgcggttgt cgtactccac aacggcatcg gctacctgct
     4741 cggcttcttt gccgccaaat ggaccggcct gccttatgat gcacaaaaaa cgctggccat
     4801 cgaagtcggt atgcaaaact cgggcctggc cgccgcgctt gccgccgcac actttgccgc
     4861 tgcgccggtc gttgccgttc cgggcgcatt gttcagcgtg tggcacaata tctccggctc
     4921 gctgctggca acttattggg cggccaaagc cggtaaacat aaaaaaccct aaacgccttt
     4981 catcataatg ccgtctgaac ggggcaaaca aaaccggcgg tttccgcccg accccttcag
     5041 acggcctttg tttgaaatcg gcgggaacga ttgcccggct gctccgattc tgctgcctgc
     5101 gttcgccccg cctttcaagc cgcctactcc cttacataaa ccacttcgac atcgtagtcg
     5161 tcttcgttcc aatcgtcatc gtcgtccgcg ccgaatttgt cctgccattc ctcttcattg
     5221 ctcaaagacg aatccaaacc ggcttcaaga cggaggacag acaacaaatg atatatgtcg
     5281 tccggaatcg gtgcttcaaa cgaaacggtt tcgccggttt tcggatgagt gaaacttaag
     5341 cggtaggcgt gcaatgcctg acgcgcaccc aaacttttaa cggcttcttt caccgtgtcg
     5401 ccgcacggat ggcgcgggtt gccgtaaacc gggtcggcgg caagcggatg gttggcctca
     5461 cgcatatgga cgcggatttg gtgcgtcctg cctgtttcga gcgagcattc gatatagctg
     5521 tgcgccagat agcgttccaa cactttgacg tgggtaacgg ctggtttgcc gccgaatttg
     5581 acgactgcca ttttcaggcg gttgtgcgga tcgcgtccga tttgggtttc gattttaccg
     5641 tcaaagggaa cgatgccgtt ggcgacggcg cggtaaatgc gtttgaccgt gcgctcttga
     5701 agctgttgca cgagggaatt ttgcgccggc agggttttgg caaccaccat cagcccgctg
     5761 gtttctttgt ccaaacggtg tacgatgcct gcacgaggta cttggctcaa ttcgggacaa
     5821 tgcgccaaga ggccgttgag cagcgttccc gtccagttgc ccgccgccgg atgcaccacc
     5881 agtccggccg gtttgttgac gacgatgacg gtatcgtctt cgtaaacaat atccaaagcc
     5941 ataggctctg gaacaaacgc cagattttcc tcactcggac ggacggttac acaaatttgc
     6001 tcgccgccta tcattttgtc tttgggttgc gaaggtttat cgtttacaat aaccgcgcct
     6061 tctttaatcc atgatgtcag gcggctgcgc gagtagtcgg gcagaagttt cgccaacacc
     6121 gcatccagcc gcccgccggc aagctcgagc ggaacagtca gacaaataca gttttcggtt
     6181 tccggggctg acacaaagtc tgaatcatcg ctataatcgg attcattatc aaaggaagta
     6241 ttctgcatga aaaaaattct tttaacggtt tcattaggtt tggcgctgag tgcctgtgcc
     6301 actcaaggta cggtagataa agatgcccag attacccaag actggagtgt ggaaaaactc
     6361 tatgccgaag cccaggatga gctgaacagc agcaattata ctcgagctgt caaattatac
     6421 gaaatcttgg aatcgcgctt ccccaccagc cgccatgccc ggcagtcgca actggatacc
     6481 gcatacgcct attataaaga cgatgaaaaa gacaaagctc tggcggcaat cgaacgcttc
     6541 cgccgccttc accctcaaca cccgaatatg gattacgcgc tgtatctgcg cggcttggtg
     6601 ctgttcaacg aagatcagtc tttcttgaac aaactggcct cgcaagactg gtccgaccgc
     6661 gacccgaaag ccaaccgcga agcgtaccag gcgtttgcgg aactcgtcca acgcttcccg
     6721 aacagcaaat acgccgccga tgcaaccgca cgcatggtca aactggtcga tgcactgggc
     6781 ggcaatgaaa tgtcggtggc gcgctactac atgaaacgcg gtgcatacat cgccgccgcc
     6841 aaccgcgccc aaaaaatcat cggcagctac cagaatacgc gttatgtcga agaatcgctc
     6901 gccatattgg aacttgccta ccaaaaactc ggcaaaccac agcttgccgc cgatacgcgc
     6961 cgcgtgttgg aaaccaactt cccgaaaagc ccgtttttga cgcacgcttg gcagcccgac
     7021 gatatgccct ggtggcgtta ctggcattaa gcccatcttc gtaatgccgt ctgaagccct
     7081 ttcagacggc attttatgtt tcaaacctca ttcaaacggt tttttctgcc aatagaattt
     7141 ataccctctc aaacgttggg ccttcacgcc gccgcgtccc aagccgaatt gcagtgcgcc
     7201 cgacaaatcg gtacgcagca gtttgatgcc gtgtgctcgg acacggttct gcaccgcttc
     7261 ggtcggatgt ttgtaggcgt tggcataacc gcttgaagca acggcatatt cgggcgagac
     7321 ggcattgagg aacacgcccg acgaggacgt attgctgccg tgatgtccca acaccaaaac
     7381 ctggctgtac aggttgcctc catacttgcc gaccaggctt tcctcaccct tcgtatccaa
     7441 atcgcccgtt accagcagtg ccgcaccatc agccacaaca cgcaaaacac aacttttccc
     7501 attatcatcg atgtttttgc gttcagacgg catcaaaaac tcgaaatcca cgccgtccca
     7561 ttgccaacgc tgttccgcac aatgccgcgc cccctcataa aattccggct gtccggcata
     7621 aatcccgccg ttcggtatct tgccgactgc ctgaaaaccg ccgtcgtggt cgctgtcgtg
     7681 atgcgacaaa accaaataat ccaaatacct gattcccgac gcattcaaac tcggcacaat
     7741 ccccatctgc gccgccgctg ccgttccggt gtcaaacaaa agatgatgat ttgccgtctg
     7801 aaccgacacc gacaaaccct gccccgcatc ccaaaccgta accgcaacct cattttccgg
     7861 cacgccgggt gaacggtaaa acacaaaccc tgccaacagc aacaccgccc acggacgcaa
     7921 acccaagccg cgcggcagca gcaacagcaa agcggcacac accgccaaca ccaacagcgg
     7981 caaaggtgcg gaggcaacgg caaactcggg cgacacatcg gcaagccaca ccaaaaaccg
     8041 caaagtatat tccgccaaaa atgcccccaa ctgttgcaaa ggcgcaaacg gcacgaccga
     8101 acccagcaac gccagcggcg tcaataccca agaaaaccac ggaattgcca ccgcattgac
     8161 caaagggctg attaaaggca gcgaagcaaa cagataaccg agcaaaacca gcgacaacac
     8221 cgaagccgcc cactgcccgc gcaaagcagt ttgccgtttt ccctcgtgca aacgccccga
     8281 gcacgcccat atcagggccg ccaccaaacc gaaagacagc caagtcccca cacccaagac
     8341 cgccgacggg tcgaacagca gcaccgccgc caacgcctgc caccacgtcg cccacgccga
     8401 caatcttccc ctgcgccaag cccacgcaaa cgccgccaac atcaaaacgc tgcgctgcgt
     8461 cggcacggaa aaaccggcaa gcagcgcgta aaacagcgcg cctgcacacc ccgccgccaa
     8521 aacccacgcg cgcggccggg caggaaggcg cggggaacag gcaagcagcc gcttcgccag
     8581 ccacgcaaac attaccgcca ccatcgtaac gtgcaaaccc gaaatactga ccaaatgcgt
     8641 cagccccaac ggccggaacg cctgccacaa ttcggggcgc aatgccgact gttcgcccac
     8701 gctcaacgcg cgcatcagcc cgatgccgtc tgaaagcccg ccgtccgcat ccgcctgctg
     8761 ccaattacgg ctgatgcggc tgcgccaaac cgcaatcccc caaccgcttc cgccatgcag
     8821 caaaaccctg tccgcaccga ccgtccccgc gccgcctatc ccgttggata atgcccacgc
     8881 ctcacggttc aaaccgcgca ggttcaattc gccgacgacg gggtgcacac gtgccgttat
     8941 ccgccatctg ctcccgaccg cccattcgcg ccgtttgtag tccgacagca gcaaatcaaa
     9001 cgtccgacca ccgctgtcca cagcctttgc cgcaaactgc aca
//
LOCUS       DAWHRA010000084         8953 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWHRA010000084 DAWHRA010000000
VERSION     DAWHRA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.084"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(149..694)
                     /locus_tag="WIB30_001437"
     CDS             complement(149..694)
                     /locus_tag="WIB30_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ3797492.1"
                     /translation="MSKIICLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAANKTATGIEALSTPKNKRWCQ
                     VLGKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAAKV"
     gene            complement(837..1358)
                     /locus_tag="WIB30_001438"
     CDS             complement(837..1358)
                     /locus_tag="WIB30_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797493.1"
                     /translation="MECHADVWHFILEKNMKKFYFVLLALGLAACGQEQSQKADAEQY
                     FFANKYQFADEKQAFYFERAARFRVLQQGLGGDFERFLKGEIPNQENLAKYRENITQA
                     VAYYADTNGDDDPYRVCKQAAQDAEILMKSMVTSGGGGTTDLDKESYQNYRKSMQECR
                     KTITEAEANLPKK"
     gene            complement(1360..2519)
                     /locus_tag="WIB30_001439"
                     /pseudo
     CDS             complement(1360..2519)
                     /locus_tag="WIB30_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            2565..2822
                     /locus_tag="WIB30_001440"
     CDS             2565..2822
                     /locus_tag="WIB30_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ3797494.1"
                     /translation="MKEFPDLEKHLIMLEVAVQDLEDRSLADSFVLSWLLQRITRQEP
                     TSIEQVRSFLQAQAKTFEPDSVQREHLESLLELVESAQELA"
     gene            complement(2889..4862)
                     /locus_tag="WIB30_001441"
     CDS             complement(2889..4862)
                     /locus_tag="WIB30_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3797495.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DARCAVVAAVLGIKYQYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRTAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVVKCIDDYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPDMLSA
                     QELATMFMPQEVRKVQRGWLDLFNNSYFSVELAEYHKDEVRVSYDLSDASVVNVFDMD
                     GKFITKAQANGNTREAFPTARIDQLAENRRKGKIKRAENAIKLANAEVNPALEQAAVW
                     DELGHLGGNDIEAEYAVLPKTGTDDFVLFEADR"
     gene            complement(4864..5115)
                     /locus_tag="WIB30_001442"
     CDS             complement(4864..5115)
                     /locus_tag="WIB30_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3797496.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            5348..6061
                     /locus_tag="WIB30_001443"
     CDS             5348..6061
                     /locus_tag="WIB30_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ3797497.1"
                     /translation="MRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNAVSDD
                     LNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGKEHIT
                     LEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDKAETY
                     LKKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVESLTEL
                     SDEDLQKTYAAVSTKKRKK"
     gene            6224..6436
                     /locus_tag="WIB30_001444"
     CDS             6224..6436
                     /locus_tag="WIB30_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3797498.1"
                     /translation="MNHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAP
                     FEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            6658..7236
                     /locus_tag="WIB30_001445"
     CDS             6658..7236
                     /locus_tag="WIB30_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3797499.1"
                     /translation="MFRHNGEILLSGINIKLNSGDSLLIRGPSGCGKTSLLRVLAGLW
                     PFGSNGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELIEAMNTCRLGYL
                     VDKLDKTDDWQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALDEPAEAALYRALKQ
                     KLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            7341..8009
                     /gene="hda"
                     /locus_tag="WIB30_001446"
     CDS             7341..8009
                     /gene="hda"
                     /locus_tag="WIB30_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ3797500.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            8006..8674
                     /locus_tag="WIB30_001447"
     CDS             8006..8674
                     /locus_tag="WIB30_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3797501.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT         2279 a         2543 c         1895 g         2236 t
ORIGIN      
        1 gatttaccca agcgcaggct cggatgttgt ttcaaagagc ctacccgaac agggcttggc
       61 gtgattttga tttcaggtaa cggtggtacg ccaaaatcgt ttttcaactt tgcacaatgg
      121 gcaacacata aagcaatcaa agactttttc ataccttcgc cgctcccaat tccatcgcaa
      181 tcgcgtccgc aatcgcgcgg cagatgcccc atttggtcgt cttaaacaag gccaaatcag
      241 tgtcgttgct gataaaaaac ggctcaaaca caatgccgcc ggcttgtgca taagctaggc
      301 gcgaatgctg gcccgcattg tcgggtttaa agccgtcttc gccgcgcagt ttccagccgg
      361 ttttcttggc aacggctttg cccagcacct gacaccagcg tttatttttc ggcgtggaca
      421 aggcttcgat gcctgtcgcc gttttgttcg ccgccgcatt ggtgtggaac tcaatcgcca
      481 catccgagcc gcgaatcagc ttgaccgcat cgcgcaaggg catattgcct ttgccttcgc
      541 cgtcggtgcg tacggtcagg ccgtaatcgt tacgcaggat tgaagccaca atgttgcgca
      601 tatcctgcgc caagtccgcc tcacggtcgc tgccgttgac tgcgcccggg tcggtgttac
      661 tgtgtccggc agtcagacaa ataattttgc tcattaaagc ctccctcaaa atcagattaa
      721 aatgcacttt cagaggctta cattttcaaa cggcatggct tttgcagcgg gcgaaacagt
      781 gtcagtaggc aacaaaaaag ccgcctgttg ttcagacggc cttagaatcg tttattttat
      841 tttttcggca aattggcttc agcttccgtt attgttttac ggcattcttg cattgatttt
      901 cggtaatttt gataactttc cttatctaaa tcagttgtac cgcctccacc gcttgttacc
      961 atactcttca tcaggatttc tgcatcttgc gcagcctgtt tgcagacgcg gtatgggtca
     1021 tcatctccat tcgtgtccgc ataataagcg actgcttgag taatattttc acgatacttt
     1081 gcaagatttt cttgattagg tatttctcct tttaaaaacc tctcaaaatc cccgccaagg
     1141 ccttgttgca atacacggaa acgggcggcg cgttcaaaat aaaaagcctg tttctcatct
     1201 gcaaattgat atttattggc aaaaaaatac tgctccgcat cagctttctg cgattgttct
     1261 tgcccacacg ctgccaaacc caacgccagc agcacaaaat aaaacttttt catatttttc
     1321 tccaaaataa aatgccatac atctgcatgg cattccattc taaaacaaat tctcctgctc
     1381 cgcttcctta tccgcctgct ttaaaattcg ccacacatgg cggtcgctta ggcggtgtgc
     1441 caaggccaaa tcgttaacgg cctcataagc aggggtaccg cctgccgtct gccggtcaaa
     1501 ctgactgcgg attttacggt ttcgcaactc atacaaggcc gtctcgcaac ggggtataaa
     1561 cagattgcac ggagcagccg accggccgcc tcactgccga taatctcttc caagtatgca
     1621 acacgggatt gactgttttt cgtataaccc tgccgcaaag gataagtcgt accgcccatc
     1681 aggcgcacca gctccagcgt ttcattaaac ccgatgaccg taatcaacgc ttgtacacta
     1741 tcagggagca gatgcttgac cgcatcaggc aaagccgctt tcgcaatctc aaagagttcg
     1801 tcatccggca aagattcgcc caaattcagc gcaacagaca cgcggctgta aagctgtacc
     1861 agctcgccat gcttaccgcg caaattcgca accagtcggg gcataccgct caacaccaag
     1921 ccgcagcccg tctcatcgtg cagacggcgt acaatttcaa gggcgcgcaa gggcaggttt
     1981 tccgcttcat cgaccacaat cagacggccc gaatcgcgca ggcggtcaga tacagactca
     2041 aacaaatcat tcaggctgcc catcgccgat accttcgccg ccgtcgccaa cttgcgcatc
     2101 aagaccaaag ccgtaaagct cggattagcc tcaattaaga tggctgcagg gtttttctcg
     2161 cagtagtttt tgaccgcctg agtcttgccc aaacccgcct gaccgtagat caccactatt
     2221 tcgccgcctt cgtgcgcatc gcgcatcgct tccgcaatac ggcgtgtcgt cttagtcgat
     2281 acaaatccca acaccagctc ttcacgtcgc gccttacttt cctgcatctc caaaaacgcc
     2341 tcgattttcg tctcgatggt ttcataattg ccgcctttat cggcataagt gccgttcaga
     2401 tacatactga tggatgccgg cgaagtatcg ataccgcgtg ccagttgggt ttggttcatg
     2461 cctgattttt ctttaaattc aaccagtttt tgttgcaatg cttgattaat ttgtttcatt
     2521 tttaatatcc ttgagtttta aacaaccttt aaaggccgtc tgaaatgaaa gagtttcccg
     2581 atttagaaaa acacctgatc atgctggaag tcgccgttca agacttggaa gacaggtcac
     2641 ttgccgattc tttcgtactt tcctggctgc tccagcggat tacccgtcaa gagccgactt
     2701 cgattgagca ggttcgcagt ttccttcaag cgcaggcaaa aacgtttgag cctgattccg
     2761 ttcagcggga acaccttgaa tctttgcttg agctcgttga atccgcccaa gagctcgctt
     2821 gagtttcaat ttttcaacaa gctcggccga aaacgctgca ttttgctgtt tgtccatcat
     2881 gttttccttt atctatccgc ctcaaacaac acaaaatcgt ctgtgcccgt tttcggcaat
     2941 accgcatact ccgcctcgat gtcgtttccg cccaaatgtc ccagctcgtc ccaaactgcc
     3001 gcctgttcca aagccggatt gacttccgca tttgcgagct tgattgcatt ttccgcccgc
     3061 ttgattttgc cttttcggcg gttttccgcc agttggtcga tacgagccgt cgggaaagcc
     3121 tcgcgggtat tgccgttggc ctgcgcctta gtgataaact tgccgtccat atcaaacaca
     3181 ttgaccaccg acgcatcact caaatcgtag ctgacccgta cctcgtcttt gtgatactcc
     3241 gccagctcga ctgaaaaata agagttgttg aacaaatcca gccaaccgcg ctgtaccttt
     3301 cgcacctctt gcggcataaa catcgtcgcc agctcttgcg ccgacaacat atccggcgcg
     3361 ataccgtcct gttccagcct catttcccga taagccttag gcgtataatg cccgccgtcc
     3421 ggatgtcggg gcagctcgcc gtgcgggcgg ttgttgtaat catcgataca cttgaccaca
     3481 tccgcgataa aacgcgacca gctcggcagt tttttcaaat atttctgttg ttcctccgtc
     3541 aaatccttgc ctttttccaa agcattaaaa gcactttcca tcttgcggta catcaggttc
     3601 ttggtactgc tgtccatccc cgcgcccgca aacgtctcat actggcgcgc catctcaatc
     3661 agattgtctt tccaccatcg ctcgatgatg cctcgacctt gcgggttgcc cgcgataccc
     3721 gtttcatgcc ggatacccaa tcgggacgta atacccgtga tttcatggtc tatcgtcttg
     3781 ccggtttggc cgccgccgtt atccgagtag tagatgatcg gcaaaccaaa atgcttgacc
     3841 ccgatacgca gagcgtccga taccgccaca caactttcag cgagagagac cgaaaaaccg
     3901 accacaaacc gcgtacaacc atcaataatc accgtcactt ccggcttaaa cggcctgccg
     3961 tgtaccggat gcgccacctt cgccttaaag ctgtggccgt cgccgatcca aacatcgtta
     4021 ggcttcaaag ccccccaatc acgtttcaca taaggcagca gcgatttata agccgccccc
     4081 gttttcctgc cgcgctcctg cataatcaac gggagtttgt cccaaacgcg ccgcaccata
     4141 ctcaagttag gcacatcgtt aaccggcata ttttccgctt cagcccactg cacaaaccgg
     4201 cggtagctgt gcgccagctt tggcgcggac ggaatattgt gaaactgcat aaacatcggc
     4261 aaccaaccgt agctctcaat cggcttaacc gccttcgtcg tcttcggagc caaagcaacc
     4321 aaccgctccg tcgcgttttc cgctttcaaa taagcagaaa tccagccgtc taaagtacgt
     4381 tcgccaacct tcgccgtccg gctgcggtca ttggcctttt ccaagttccc aagcgtgacc
     4441 gcgtccaatt taccttctgc cagcaagccc aaaaactgag ccaccgcagc cttggcagag
     4501 caatcgtatt ggtatttaat ccccaacacc gccgccacca ccgcacatcg cgcatccgcc
     4561 accgaccgtt gtttctcgtt caacagcttg gccgcttcag ccagtgcttg agccgacatc
     4621 gccgtccccg gtctgacttg gggcagggtt ttcggcatct tctccgccag ctcgtccgac
     4681 tgccgtttca tgatggctgc tcggatttcg gcagggaggg catattcaac accaccaccc
     4741 ttaccttgac gccttctcgt ttgccaacca tccctttgcg ctttttttct cagccctatt
     4801 actgttcttg gcatatttgg caacccaaaa ttaagcaggc ttttgagatc catatattcc
     4861 atttcaaccc actccgagca tctctttcaa ttcagctatc tttttgtctc gacaatctgt
     4921 tttcttgtaa tgcggaaaaa cttcaactaa atctttttga atcactttcg cgattgcctc
     4981 cgcaattctt ttgcttcctc gtccatttct tatgacctca gaaacagatt ggttagttac
     5041 ccctaaagcc gaagccaaca ttcttgccga tatatttttt tcttctaagg atttatagat
     5101 ttcgttcgct tgcataatgc ctccatgata gaatccatta cttacttacc ttacaggctt
     5161 taatgcctgc cttaactgtt acctggatga tatacccaaa atatttggta taccaaatat
     5221 atttggtact attttgcggt gttttatgga acgcattgat ttagcattga atttagttaa
     5281 agagagaagc cgacttggat actctcgaaa gaattttgct gagcaaactg gatgcagtgc
     5341 agaaagtttg agactgtatg aaagcgggca ggtcaacatt cctgcggact ttttacttgc
     5401 tgctgtccaa ttaggggtgg atattcaatt tgtctttaca ggcattcatt cggcaaattt
     5461 aaatgctgtt tcagacgacc ttaataaaaa attggacgac gctattggca aatcaaacgt
     5521 tgagaatcag attaacggca acgtcagtaa cgctgttatc gcctcatctg gttcggttgt
     5581 tcatcaaatt aatacccaaa atcacgtcat cagaacccgt gtcgattcaa aacccggcaa
     5641 ggaacatata acgcttgagc aagcttcaaa gctgcaacaa cttgttaaac aagttgccgc
     5701 agcagaagag attgccaaac ggtcgccaaa atcaatccgt gcaatttggg ctagtctgaa
     5761 tgctcactgt aaagttccaa gttacaaact aatcgcattg agcgattacg acaaagcaga
     5821 aacttatctc aaaaaatggc tgggtaggtt gagcaataca gcaacatcaa agaacaacga
     5881 tcccgactgg cgaaaaaaga aatacgctta tatcaaatta aatgtaaaac agcttgagct
     5941 tgaagattgg ttgaaaagtt acctcgaaaa aaatttctca gtagaaagcc tgactgaatt
     6001 aagtgatgaa gatttgcaaa aaacatatgc agctgtatcg actaaaaaac ggaaaaaata
     6061 ggcttaaaca tgaaactccg cctttaaatt caagtttcat gtttagctta tggtcttcag
     6121 atgctttttt tagatgtatg atattaaaag attttttcaa acatggaact attatacaag
     6181 ttccatgttc agtttctagt taaagccttt tataaacatt ctaatcaacc acgccgaaac
     6241 cgagccgcgc gacggcctat atgtcttgcg tataggcaat gacctctttg tcaaacgtgt
     6301 gcaacgtata ccgggcaaac tgcttgtaac gtctgaaaat ccacgttacg caccgtttga
     6361 aatagatttg agtaataccc aagacgacat cgccatcata ggccgtgttg agtggtatgg
     6421 ccggagtatt gattaaatcc attttaaaaa cctcttaaaa cagttttaaa aattccctaa
     6481 tctcgtcagc tttaaacaga aaaccgcgca ttccggcgcg gttttatgaa aaagctggcg
     6541 taacttttcc ggatacaaaa aaacgccgaa atccacgtct tccgaagatt tcagcgtttt
     6601 tttactctat ttgttccttg tgcaaaaact aacagtcccc cacaggaaaa cgtcgctctg
     6661 ttccgccaca acggcgaaat cctgttgagc ggcatcaaca tcaaactcaa cagcggcgat
     6721 tcgctgctga tacgcggccc gagcggttgc ggcaaaacct cgctgctgcg cgtgctggct
     6781 gggctttggc cgttcggcag caacggcaaa gtcagccgtc cgccgcatca agacatcctc
     6841 ttcctgccgc aacgcccgta cacggcacag ggcagcctgc gcgacgcggt ttgttatccc
     6901 gacatcgaca aacagcatcc ggagctaatc gaagccatga acacctgccg cttgggttat
     6961 ctggttgata aattagataa aaccgacgac tggcaacaca aactctcccc gggcgaactg
     7021 caacgcgtcg ccttcgtccg cgccctgctt tccaagccca aaatcgtcct gctcgacgaa
     7081 gccaccgccg ccttggacga accggccgaa gccgcgctct accgcgcctt aaagcaaaaa
     7141 ctgcccgaca gcatcatcat cagcatcgga caccgaagca cgctcaacgc gttccacaat
     7201 atgcgcctcg atgtcggcaa cgttgcctgc ggttgaacga tgccgtctga agccgttttc
     7261 agacggcatc gttcaaattg gaaaggcgcg cgaaaacaac gtacaataaa cccgtttacc
     7321 acccctcgga atgccgaacc gtgaaccagc ttattttcga ctttgccgca cacgactatc
     7381 cgagtttcga caaattcctc ggcacggaaa acgcggaact ggtctatgtc ctccgacaca
     7441 aacacggaca gtttatctat gtctggggtg aagaaggtgc gggcaaaagc catcttttgc
     7501 aggcgtgggt cgcacaggcg ctcgaagccg gcaaaaacgc cgcctatatc gatgccgcct
     7561 ccatgccgct gaccgatgcc gcgttcgagg cggaatacct cgccgtcgat caagtcgaaa
     7621 agctgggcaa cgaagaacaa gccctgcttt tttccatctt caaccgcttc cgcaacagcg
     7681 gcaaaggctt tttgctgctc ggttcggaat acacgcccca gcagctcgtc atccgcgaag
     7741 atttgcggac gcgtatggcg tactgcctcg tttacgaagt caagccctta accgaccaag
     7801 aaaaaatcga cgcgctcgcc agtatggcgg cggcacgcca agtaaccgtc gattccgaaa
     7861 ttttcgaata cctgctcaaa cactggcggc gcgatatgga cagcctgatg atgatgctcg
     7921 acacgctgga caactacgcc gtcacaatgg gcaaacgcat caccctgccg cttttgcgcc
     7981 agcttttgaa acaacaggaa acccaatgaa aaacctcgcc atcttcgacc tcgacaacac
     8041 cctcatcaac accgattccg accacgcctg gccgcaatac ctcatcaaaa aaggcttggt
     8101 tgacgctgcc gaaaccgaag cgcaaaacga aaaattctac cgcgactacc aaaacggctg
     8161 cctcgacatc gacgcattcc tcaaattcca cctcgccccg ctcgcccgtt acagcaaaga
     8221 agagctggcg gaatttcacc gcgaatttat ggcggaatac atcatccccc acatctcgcc
     8281 tatgcagcgt atgctggtgc agagccacca aatggcaggc gacgaaaccc tcgtcatctc
     8341 atcgaccaac gagttcatca tcacccccgt ctgccgcctt ttcggcatta ccaacatcat
     8401 cggcacacaa ctcgaatccg gttccgacgg ccgctacacc ggcaattaca tcggcacgcc
     8461 cagcctcaaa gaaggtaaaa tcacccgcct gaaccaatgg ctcgccgaac gcggcgaaac
     8521 gctcgaaagc tacggcaaaa cctattttta cagcgactcc aaaaacgacc tgccgctgct
     8581 gcgcctcgtc gacgaacccg tcgccgtcaa ccccgatgcc gagctggaaa aagaagccaa
     8641 agaaaaaggc tggccggttt tgaatttcaa atgacgcaat gccgtctgaa gcccgattcg
     8701 gcgttcagac ggcattgagg ctgaaatcca aaaaactcaa agccacagga atttatccgg
     8761 aaaaacaaaa aacacaggaa ttcatcggga aaaacaacaa tctttccacc gtcattccca
     8821 cgaaagtggg aatcttgatc cgtccgcacg gaaacttata tttcgtcatt tgcacccgac
     8881 ataattcaat actttatttt gcaataaatt aacggatgct gacaaattag attggattaa
     8941 ttaccctatt atc
//
LOCUS       DAWHRA010000085         8692 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWHRA010000085 DAWHRA010000000
VERSION     DAWHRA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.085"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            28..3642
                     /gene="recB"
                     /locus_tag="WIB30_001448"
     CDS             28..3642
                     /gene="recB"
                     /locus_tag="WIB30_001448"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ3797502.1"
                     /translation="MSASIQAFDPLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDGALSDGIAAYCAE
                     HHKGDTFLPTLLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGYD
                     LNALEEAEEATIIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRQTYAQEKDGITMLKQNWRRVADNAPAGTDFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVAAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEMWLPAVAEM
                     AEACQKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPEGNICVIDYKSNHLGADASAYTRQAMDEAVAHH
                     HYYLQALIYAVASARYFKLRGQPPAAVSVRYLFLRGIDGKGGGVWHWDIDAAALEQIK
                     "
     gene            3681..4040
                     /locus_tag="WIB30_001449"
     CDS             3681..4040
                     /locus_tag="WIB30_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ3797503.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            4172..4651
                     /locus_tag="WIB30_001450"
     CDS             4172..4651
                     /locus_tag="WIB30_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ3797504.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAAHRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(4926..6305)
                     /gene="radA"
                     /locus_tag="WIB30_001451"
     CDS             complement(4926..6305)
                     /gene="radA"
                     /locus_tag="WIB30_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ3797505.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(6491..7555)
                     /locus_tag="WIB30_001452"
     CDS             complement(6491..7555)
                     /locus_tag="WIB30_001452"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ3797506.1"
                     /translation="MISLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(7709..8149)
                     /locus_tag="WIB30_001453"
     CDS             complement(7709..8149)
                     /locus_tag="WIB30_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797507.1"
                     /translation="MGSSDSFKEKKEIFEIGTPAYRQKLIDVWKKSINGNEKSWVLFE
                     NGTCVILLEPEKDLAKQAKEMLSKWGKVQIGTPSADFGIITLDSGDGYAVSCHHPEIF
                     TLILKEEGLDEDFKIGIEGRSHRDCDAEEPKVIHIEDKRTIETP"
     gene            complement(8182..>8692)
                     /locus_tag="WIB30_001454"
     CDS             complement(8182..>8692)
                     /locus_tag="WIB30_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ3797508.1"
                     /translation="LADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLGLYADAV
                     KWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLLMYLGRL
                     AYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDEIGEPQKAEAQRNLVLEAVSDDE
                     RHAALEQHS"
BASE COUNT         2005 a         2620 c         2271 g         1796 t
ORIGIN      
        1 cgtccaaccg ttttccgagc acgcactatg tctgcaagca tccaagcatt cgacccgcta
       61 accgtcccca ttgccggcac caacctgatt gaggcctctg ccggcaccgg caaaacctac
      121 ggcatcgccg ccctgtttac gcgcctgatc gtattagaac aaaaaaacgt cgagcgcgta
      181 ttggtcgtta ccttcaccaa agccgccacc gccgagttga aaacacgcct gcgcgcgcgt
      241 ttggacgatg tgttgcaggt tttagaaagc aaagaaattg ccgaacttgg agacggcgcg
      301 ctttcagacg gcatcgccgc ctactgcgcc gaacaccaca aaggcgacac cttcctgccc
      361 acactcttaa aacaggcttt gcaaaaagag ggccggacgc gtctgattgt ccgcctcaag
      421 gccgccatcg ggcaattcga caacgccgcc atctatacca tccacggctt ctgtcagcgc
      481 atcctgcgcg actacgcctt cctgtgccaa gccccgttcg atgtcgaact gaccgaagaa
      541 gacggcgacc gcctgcttgt cccggcgcaa gatttttggc gggaacgcgt cagcaatgat
      601 ccggtgcttg ccgcattggc gtttaaacgc aaagctgtgc cgcaaaccgt ccttgcccaa
      661 atccgcgcct acctgtcccg cccgtacctg aatttccgcc gtccgcaggc ggatttgaaa
      721 caggcgcagc gcaacgccga aacctcatgg caaaccgtct gccgcctgct gcccgagctg
      781 gaagccggct tttggcgcat ccatcccgac ctcaacggca acagttaccg caaaaacagc
      841 ttcggcaacc tttttaaaga actggcacaa aaatccgccg ccggacaact gccctttctg
      901 gacaaagaca cgcacgacag actcttgaaa cttgcgtccg acaaactcga agccggactg
      961 aaaaaaggca aaacgcccga tgcggcagta tttgccgaat tgcagaaact ggcagacttc
     1021 gggtacgatt tgaacgcact cgaagaagcg gaagaagcaa caataatccg gctgcaactg
     1081 gatttaatcg gatacctcaa ccgcagcctt gccgagatga aaaaatcgcg ccgcgaacgc
     1141 ggtttcgacg acctgctgct cgatgtccac accgcgctga ccgacaatcc gcacgccgaa
     1201 accctcgccc gcgccgttgc cgaaaactgg gaaaccgcgc taatcgacga gttccaagac
     1261 accgacccgc tgcaatacga aattttccaa aaaatcttca tcgcccaaaa ccgcccgctg
     1321 tttctggtcg gcgatcccaa acaggcgatt tacagctttc gcggtgcgga catttacgcc
     1381 taccttcagg cggcggaaga tgcgcggcac cgctacacgc tcgccaccaa ctaccgcagc
     1441 cacgccgcgc ttatcggcag cataggcgcg ctgttccgcc tcaaagaacg cccgttcgtt
     1501 ttggaaaaca tcggctattc ggaagtcggt gcggcgcgtg ccgaaagcag gttgtccccc
     1561 gaacgtcctg ccgtacaggt tcgttggctg cacgaaaacg acaatgaaaa agccaacaaa
     1621 gacgttttgc gccgccgtgc cgccgactat tgcgccgacg aaatcgccca cgcgctcaac
     1681 gaagccgccg gaggccgtct gaatttcaaa ggctgcccgt tgcagtcggg cgatattgcc
     1741 gtgctggtac gcacgcacaa cgaggcggtg atggtttccg ctgccctgaa aaaacggcag
     1801 gtgcaaagcg tcctgctttc gcgcgaatct gtgttcgcct cgcccgaagc cgccgccctt
     1861 tccgcactga tcggcttctg gctcgaaccg cgccgcgccg gaacgctgcg ctttgtcctg
     1921 acaagcggca tattcggtta tgacgcgcag caattgcacg acttcaacca aaacgaaagc
     1981 gagattttgc attgggcgga atctgcccga accgcactcg acaactggaa tcaatacggc
     2041 attttcgccg ctatgcagca attttcccaa acacacggca tcgaaacacg cctcttaagc
     2101 cggaacaacg ggcgcagcct gaccaactat ttccaactgc tcgaactgct tgccgccgaa
     2161 gacgcgcaaa accgcaaccc cgccgcgctg cacaaatggc tgcgcgacca aatcagcctg
     2221 gccggcaaca acggcggcga caaccgcgcc atccgtctgg aaagcgacga agatttggtc
     2281 aaaatcgtta ccatgcacgc ctcgaaaggt ttacagtatc cgctggtgta ctgcccgttt
     2341 gcgtgggacg cgcaagatac cgggccgtcc gactggcaaa tcctccacca aagcgcaaac
     2401 cgaaccgaac tgttggcaaa ggcgcaactg tccgaagacg aacagaaaca atacgccgat
     2461 gaagaaatgg cggaacgcct gcgcctgctt tatgtcgcgc tgacgcgtgc cgaggaacag
     2521 ctcaacatct acgccgccta ttcttccgat accgccgaca accccctcgc ctatctaatc
     2581 gaaggcttgc cgcaagacag ccgcgaaacc gtccgccaaa cctatgccca agaaaaggac
     2641 ggcataacga tgctcaaaca aaactggcgg cgcgtggcgg acaacgcccc ggccggcacg
     2701 gatttcgcct tcaccgaaaa tgcgccgccc cctgccgcct atcgcagcaa cgccggtcaa
     2761 accgccgaat ttgccgcaaa cagcattccc gaacgcagat tccgatttgt ccgccacacc
     2821 agctttaccg ccttaagccg ccatacccaa acgcccgacg gcggcgaaga agatgcctgc
     2881 ccgtccttgg atgccgccga aacctcggta gcggcgatgc cgtctgaaac gccgacggct
     2941 tcagacggca tatcgataca cgattttccg aaaggcacgc aggcggggct gtgcctgcac
     3001 gaaattcttg aagatttcaa attcggacaa gcagccgccg gacaggaaac tctcattgcc
     3061 gacaagctga agaaatacgg gtttgaagaa atgtggctgc ccgccgttgc cgaaatggcg
     3121 gaagcctgcc agaaaacgcc gctgacgggg gcatactgcc tgtccgacat cccgcccgag
     3181 tgccgctgtc ccgaaatggg ctttaccctc cacaccgaag acttcggcct caaacggctg
     3241 cgcgactggt ttgcccgcga cgacatcagg ctgcccgaag tctgccgtgc cgccgccgaa
     3301 acgctcgact tccacaccgt caacggcttt ttaaacggct ttatcgatat ggtctgccaa
     3361 gaccccgagg gaaatatctg cgtcatcgac tacaaatcaa accatctcgg cgcggacgca
     3421 tccgcctaca cgcggcaggc gatggacgaa gccgtcgcgc accatcacta ttaccttcag
     3481 gcactgattt acgccgttgc ctccgcgcgc tacttcaaac tgcgcggaca accgcccgcc
     3541 gccgtttccg tccgctacct gtttttacgc ggcatagacg gcaaaggcgg cggcgtttgg
     3601 cattgggaca tagatgccgc cgctttggaa cagataaaat aacttcccgt cccttaagac
     3661 gacaaccgca ggagaaccct atgaatatca aacacctgat taccgccgca ctcattgcct
     3721 cagccgcctt tgccgcgcag gcagccccgc aaaaacccgt atccgccgcc caaaccgcgc
     3781 aacattcagc cgtttggatc gatgtccgca gcgaacagga atttagcgaa ggtcatttgc
     3841 acaacgcggt caacatcccc gtcgaccaaa tcgtccgccg catacacgaa gccgcgcccg
     3901 acaaagacac gccggtcaac ctctactgcc gcagcggacg gcgtgccgaa gccgcccttc
     3961 aagaactgaa aaaagcaggc tatacgaatg ttgccaatca cggcggttat gaagacctgc
     4021 tcaaaaaagg gatgaaatga cgcaatgccg tctgaaagga caggctttca gacggcatac
     4081 gacaccccgg cacgcggcgc aacatcgggc gcggcgcgtg ccgccccgtc cgaagcctga
     4141 taaacaaacc gaaaaggaaa aaggaaatac gatgaaacac atactccccc tgattgccgc
     4201 atccgcactc tgcatttcaa ccgcttcggc acatcctgcc agcgaaccgc aaacccaaaa
     4261 cgaaaccgct atgaccacgc ataccctcac ctcaaaatac agttttgacg aaaccgtcag
     4321 ccgccttgaa accgccataa aaagcaaagg gatggacatt tttgccgtca tcgaccatca
     4381 ggaagccgcc caccgaaacg gcttaacgat gcagccggca aaagtcatcg tcttcggcac
     4441 gcccaaagcc ggcacgccgc tgatggtcaa agaccccgcc ttcgccctgc aactgcccct
     4501 gcgcgtcctc gttaccgaaa cagacggcaa agtacgcgcc gcctataccg atacgcgcgc
     4561 cctcatcgcc ggcagccgca tcggttttga cgaagtggca aacactttgg caaacgccga
     4621 aaaactgata caaaaaaccg taggcgaata agcccgtcgc cgacatatgg cggatggaaa
     4681 tcaaacacac acgcccctca ttcccacgaa gtcgggaatc caagcggttg ggttaccgtg
     4741 attttcaatc attgccgaaa cggacgattc cggattcccg cccttgcggg gatgacgggc
     4801 gtgtgcgttt tgccatatca caggcaactt tgccgcaaat cttatctgtc gccggtatgc
     4861 cttcttgttt caatccgcca caaaccaaat gccgtctgaa acctgatttc agacggcatt
     4921 tccgtttatt ccctgctgtc acggcaaata tcgatggctt cctgcaaact cgaaacgccg
     4981 tagattttca ggttcggaaa ctctttggcg ttgcgcggca tattggcttt gggaacgatg
     5041 gcgcgtttga agccgagttt ttccgcttct ttgagccgct cttgcccgcg tgcgacgggg
     5101 cggacttcac cgctcaaacc gatttctcca aatgcaacca ttttttcggg cagcgggcgg
     5161 ttgcggaagc tggaaagcat cgcgaggatg accgccaaat ccgccgccgg ttcgccgatt
     5221 ttcacaccgc cgacggcgtt taaaaacaca tcctgatcga agcaggcgat gccgccgtgg
     5281 cggtttaaca cggcgagcaa catggcgagg cggttttgtt cgagaccgac ggtgagtcgt
     5341 ttgggggtga atccgtgcgc gtcatcgacc aatgcctgaa tttcaaccaa aagcggtcgg
     5401 ctgccttcct gcgtaaccaa aacgcacgaa ccgggcgtgt cgtcgcggta gctggcaagg
     5461 aagatggcgg acgggttgga cacacctttc aaaccgtttt ccgtcatcgc gaacacgccc
     5521 agttcgtttg ccgcgccgaa gcggtttttg atggcgcgta tcatgcggta gttggaatgt
     5581 tggtcgccct cgaaatacag cacggtatca accatatgct ccagcacgcg cgggccggca
     5641 atcgcgccgt ctttggtcac gtgtccgacc agtatcatgg cgatgcccat ctgtttcgcc
     5701 atgcgcgtca gttgggcggc acactcgcgc acctgcgaca cggagccagg ggcggacgtg
     5761 atttggtcgg aatacatggt ttggatagag tcgatgacga caacttcggg ctgatgctgt
     5821 ttcaacgccg tctgaatcgc ctccatacgg atttcggcaa gcaggttcac accttctgtg
     5881 ggcagttcca aacgttgcgc acgcagggcg acctgttggg cggattcctc gccggaaacg
     5941 tacagcactt tgcggctttg cgccattttg gcgatggttt gcaacagcag cgtggatttg
     6001 ccgatgccgg gatcgccgcc gagcaggatg accgcaccgt cgaccaaacc gccgcccaat
     6061 acgcggtcga gttcgcccat accggtcgga ttgcgcggca cttcggtcgc agtaacggcg
     6121 gagagggatt ggacggtcga ggtatccgcc gcccaagatt ggaaacgggc gtttttcggc
     6181 tcgggcgcgg caaggctttc ctgaagcgtg ttccactcgc cgcaatgcgg gcatttgccc
     6241 tgccatttcg gcgaagtgcc gccgcattcg gtgcattggt aaagggtttt aagcgttttt
     6301 gccatcggtt ttccctgcat ctgaaaacaa aaatgccgtc tgaacatagg gacggttggg
     6361 attgcgccaa tacgacaacg ccgccgtcat gtactatccg tacacggcgg atattgccgc
     6421 attgccttat ttttacccta accggccatc tctcggcttc agacggcata tcgggcggtt
     6481 tcctgccggc ttacccgccg tgatactgcc gagacagctc gtgtacggta tcgactaaga
     6541 ttttggcgtg ttcggggtcg gcgtgttggt tgatgccgtg tccgaggttg aagacatggc
     6601 cgctgccgtg tccgtagtcg gctaggatac gtgcgacctc ggtgcggatg gattccggcg
     6661 taccgaagag ggcgaacggg tcgaagttgc cttgcagggc gacttgcttg ccgacgcggc
     6721 ggcgtgcttc gccgatgtta cacgtccagt ccaagcccaa tgcgtctgcg ccgatttggg
     6781 ccatactttc cagccacagc ccgccgcctt tggcaaatac gataacaggc acgcggcggc
     6841 cttcgctttc gcgtttgagt ccggcgacga tctggcggat gtatttgagg ctgaattctt
     6901 taaacgccgc atcgctcaac acgccgcccc aagtgtcgaa aatctgcacc gcctgcgcgc
     6961 ccgcgtcgat ttgggcgttg aggtaggcgg taacggcttg ggcgttggta tcgaggattt
     7021 tgtgcagcaa atcggggcgc gagtacatca tggttttgat ggtgcggaat tctttgctgc
     7081 cgccgccttc gaccatataa caggcgagcg tgaacggact gccggagaag ccgatgagcg
     7141 gtacgcggcc gtccaatgct ttacggatgg aagttaccgc gtcgaaaacg tattgcagtt
     7201 tttccatatc gggaacgtgc agcttggcga tgtcggcctc gtgttgcagg gcgcgtttga
     7261 atttcgggcc ttcgccttcg gcaaaataca gtcccaagcc cattgcgtca gggacggtca
     7321 ggatgtcgga aaacaaaatc gccgcgtcca aatcgaaacg ttccaaaggt tggatggtaa
     7381 cttcggtcgc caattcggtg tttttgcaca aatcgaggaa gctgcccgct ttcgcgcgtg
     7441 tggctttgta ttcgggcaga taacgccccg cctggcgcat catccaaatc ggcgtgtatt
     7501 cgacaggttg tttgagcagg gcgcggagga aagtgtcgtt tttcaaagag atcatgtggg
     7561 gctttcggtt tgtttgtaaa acggggaagg aaaacggttt tcagacaacc tttgcggcgg
     7621 tttccgcaat gaaaggtcgt ctgaaaaggg agggacgggc acatttgcct aagtaatcat
     7681 cagtagatga ttaaacggca gcaggttttc atggggtttc aatggtgcgt ttatcttcga
     7741 tatggataac tttgggttct tcagcatcac aatcgcgatg agagcgccct tcgataccga
     7801 ttttgaaatc ttcatccaat ccttcttctt ttaggattag cgtaaaaatt tcgggatgat
     7861 ggcatgaaac ggcatatcca tcgccactat ctaaagtgat aatgccaaaa tctgcagatg
     7921 gtgttcctat ttgaaccttg ccccatttgc ttaacatctc tttagcttgt ttcgccaaat
     7981 ctttttccgg ttcaagtaaa atgacgcaag tcccattttc aaagagcacc caagattttt
     8041 cgtttccatt aatgctcttt ttccaaacat caattaactt ttggcgataa gcaggcgttc
     8101 caatttcaaa tatttctttc ttttccttaa aggaatctga agaccccatc aaattctccc
     8161 agataaagat atttcccaaa atcagctatg ctgctctaac gctgcgtgac gttcgtcatc
     8221 ggagacggct tccaaaacca agttgcgctg cgcctccgcc ttctgcggtt ctccgatttc
     8281 gtcgaaaacc tttgctagaa ccaaacgcgc ggaaatactc ggctttaatg caatgctcgc
     8341 ttcaaggtag ccttttgcct tgccccaaag tttgcggccg taggcgagcc gaccgagata
     8401 catcagcaga agcgcgttat cgggctgttc tttcagccaa gcatcggcaa aatcgatggc
     8461 tttctgctgt tcgcgctcgc ccaaaaagcg cacgctttcg acaaaggctt ccaaaagctc
     8521 ggggcggcgg ttgtgcggat aatgctgttt gacccatttg accgcatcgg catacagtcc
     8581 caaacgttcg tacttttccg caaccgatac gctcaattcc ccgtttttga ggctgtcggg
     8641 aatccgcttc aggcaggttt tcaaagcggc ggcatcggca gcatccgcca gc
//
LOCUS       DAWHRA010000086         8690 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWHRA010000086 DAWHRA010000000
VERSION     DAWHRA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.086"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            19..492
                     /locus_tag="WIB30_001455"
     CDS             19..492
                     /locus_tag="WIB30_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ3797509.1"
                     /translation="MTMQVFLTIPGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKK
                     YIDGVLTAGHGKLYQSDGRHSVNPTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDF
                     FEGKGIDVLYMHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(700..2085)
                     /gene="pntB"
                     /locus_tag="WIB30_001456"
     CDS             complement(700..2085)
                     /gene="pntB"
                     /locus_tag="WIB30_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ3797510.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFIGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSS
                     AASFAGQEVSEYREVKAADVAEMLKGANSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(2151..2534)
                     /locus_tag="WIB30_001457"
     CDS             complement(2151..2534)
                     /locus_tag="WIB30_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ3797511.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GAAARAHAAQQNGFEVFAPFAAAVLTAHATGNAAQSTINTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWVGGFVCTVGLFVVAA"
     gene            complement(2538..4079)
                     /locus_tag="WIB30_001458"
     CDS             complement(2538..4079)
                     /locus_tag="WIB30_001458"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ3797512.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFAVTRRMLN
                     MFKKG"
     gene            4369..5202
                     /gene="serB"
                     /locus_tag="WIB30_001459"
     CDS             4369..5202
                     /gene="serB"
                     /locus_tag="WIB30_001459"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ3797513.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SGADAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            5266..6489
                     /locus_tag="WIB30_001460"
     CDS             5266..6489
                     /locus_tag="WIB30_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ3797514.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVARASEGRRLGVLILCGMLAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLTV
                     NTSLLWFSAVIGPILGVAAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVISVWFP
                     EILGNGKAGNQLTFGGLTDWQHSLGLTAVKWLVVLMALAVGAYGGLITPSMMLGSTIA
                     FAAATAWNSVFPEMSSESAAIVGAAVFLGVSLKMPLTAIAFILELTYAPVALLMPLCT
                     GMAGAVWVAKKMGFK"
     gene            6653..8233
                     /gene="purH"
                     /locus_tag="WIB30_001461"
     CDS             6653..8233
                     /gene="purH"
                     /locus_tag="WIB30_001461"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ3797515.1"
                     /translation="MSSIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGTPEIAGFPGRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLAAYKQLQGKELSYNNIADADAAWEAVKSF
                     DVPACVIVKHANPCGVAIASNTLDAYKLAYATDTTSAFGGIIAFNREVDGATVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT         1973 a         2337 c         2425 g         1955 t
ORIGIN      
        1 attcccgcct gcgcgggaat gacgatgcag gtatttctga cgattcccgg ctatgatgtt
       61 gaggcagaaa tcgaaaaatt cgtttggatg gatgctgtga tttggcagat gccgggctgg
      121 tggatgcacg agccttggac agtgaaaaaa tacatagacg gagtattaac cgctggacac
      181 ggcaaactct accaaagcga cggcagacac agcgtcaatc cgactgaggg ctacggcaca
      241 ggcggcttgt tgcaaggcaa aaaacatatg ctttcactga cttggaatgc gccgattgag
      301 gcgtttaccc gcgaaggcga tttctttgaa ggcaaaggca ttgatgtttt gtatatgcac
      361 ttccacaaag ccaacgagtt tttgggtatg acccgcctgc cgacattctt atgtaacgat
      421 gtggttaaaa atccgcaagt ggaaaaatac ttggcagatt atcaggcaca cttggaaaaa
      481 gtgttcggct aattaaaaat ccatcttcaa cacggagatg gattttgttt gtttcgttga
      541 ttttgtgtca gtttcagatg taggtgctta ttcggacggg ccgtctgaaa atgtttgccc
      601 caatgcaaaa aaatcactgc aaaccttcat aaacggggtt tgcagtgatt ttttcaaatc
      661 aaacagattg aaaacctgcg ccgaattgtt cagacggcat tattttttca gttcggacag
      721 aatgtcatcc acggttttct tcgcatctcc gaaacacatt acgctgtttt cgttgaagaa
      781 cagcgggttt tgtacacctg cgtagccggt attcatcgag cgtttgaaga cgacgacttc
      841 ttttgccttc catacttcca acacgggcat acccgcaatc gggctgttcg ggtcggtttg
      901 ggcggcagga ttgacggtat cgttcgcacc gatgaccaag accacatcgg tttcggggaa
      961 atcgtcgttg atttcgtcca tttccaacac gatgtcgtag gggactttgg cttcggcaag
     1021 cagtacgttc atatgaccgg gcaggcggcc ggcgacgggg tggatgccga agcgtacttc
     1081 tgtgccgttt ttacgcaaaa gctcggtgat ttcggcaacg gggtattgcg cctgtgcgac
     1141 tgccatgccg tagcccgggg tgatgatgac gctgtttgcg cctttcagca tttcggcaac
     1201 atcggcggct ttgacttcgc ggtattcgct gacttcttga ccggcaaaag atgccgcgct
     1261 gctgtcgctg ccgaaaccac cggcaattac cgagacaaac gagcggttca tggctttgca
     1321 cataatgtag gacagaattg cgccgcttga gccgaccagt gcgccggtaa cgatgagcag
     1381 gtcgttggag agcataaagc ctgccgctgc ggccgcccaa ccggagtagg agttgagcat
     1441 agatacgacc acgggcatat ctgcgccgcc gatggaggca accaagtgcc agccgaatgc
     1501 gagggcaatc agggtcataa tcagcaggat gaagccgctg ccgtcaatgc cgataaatac
     1561 gagcagcaac acaaacgata cggcgagcgc cagtgcgttg agcttgtgtt tggcgggcag
     1621 ttgcagcggg ctgctgctga ttttgccgtt gagtttgccg aatgcgacca gcgagccggt
     1681 aaaggttacc gcgccgatga aaatgcccaa atacacttcg accagatgga tggtgtgcat
     1741 atcgtgcgaa acgttgcccg gctcgatgta gctgttgaag ccgaccaaaa ccgccgccaa
     1801 gccgacgaag ctgtgcagca gggcaatcag ttcgggcatt tcggtcattt ccaccttttt
     1861 ggctttgtag atgccgattg ccgcgccgat gagcatggcg atgatgatcc agcccagtcc
     1921 gtgggtattg tcggaaaaaa cagttacaaa aagggcgacc gccataccgg cgataccgga
     1981 atagcagccc tgtttggcgg tttcctgttt ggacagcccc gccagtgaga agatgaataa
     2041 aattgcggca acgatatacg ccgctgttac gagtcctgaa gacatggaaa ttctccgatt
     2101 ttcgatgatt tgttttcaat gccgtccgaa ctgttcagac ggtatctgtt tcaagcagcc
     2161 acgacaaaca gcccgacggt gcagacaaat ccgcccaccc acatcagcga gcgcaatgcg
     2221 gctttgtcgg cgatatagca ccagataaag gcgaggcgga acaggatgaa caggcccgca
     2281 agcgtgttga tggtcgattg cgccgcattg ccggttgcgt gtgccgtcaa aacggcggcg
     2341 gcaaacggtg caaagacttc aaaaccgttt tgctgcgcgg cgtgggcacg ggcggctgcg
     2401 ccttgcgtat gcgccagaaa atcgcgcgga ttgtggttgt ctttaaaccg gaatccgccc
     2461 gcttttttgg catacgccgc acaaaaaagc ggcaataggc aggcaatcag aatacaccaa
     2521 taggcgaaag tcatggctta ccctttctta aacatattca gcatacgccg tgttaccgca
     2581 aagccgccga agatgttgat gctggcaatc aggatggcga tgaaggcaag cagggtaacg
     2641 aaaccgttgc cttggctgat ttgcagcagc gcgccgacga cgatgatgcc ggagatggcg
     2701 ttggttaccg acatcagcgg tgtgtgcagc gagtggctga cgttccaaac gacatagtag
     2761 ccgatgacgc aggcaaggac gaacacgata aagtggttca ggaatgctgc gggtgcgacc
     2821 gcgccgaccc acagcaccaa tacggcggcg atgacggcgg gcgcgagttt tttccacagg
     2881 gaaacaggtt tcggctcggg cttggcggca ggcgcggctt tttcagacgg cgtttgctgc
     2941 ggctgggcgg aaacttgaat cggcggaggc gggaaggtga tttcgccgtc gtgggtaacg
     3001 gtcatgttgc ggataatcac gtcttcgaag tccaacgtga tttcgccgtc tttgttcggg
     3061 cttaacagct tggtcaggtt gaccaagttg gtggcgtaaa gctgggaaga ctgtccggca
     3121 aggcggtttg ccatgtcggt gtagccgatg attttcacgc cgttgccggt tacggacaat
     3181 tcgcccgggc gggtgagttc gcagttgccg cccgtcgccg ccgccaaatc gacgatgacg
     3241 gagccggatt tcatgctttc caccatttct ttggtaatca gcttgggcgc gggtttgccc
     3301 ggaatggcgg cggtggtgat gatgatgtcc acttctttcg cctgctcggc aaagagcttc
     3361 atctcggctg cgataaattc gtcgctcatc actttggcgt agccgtctcc gctgccgccc
     3421 gattcttgtg ggaagtcgag tttcaggaac ttgccgccca tcgattcgat ttgttccgcc
     3481 acttccaagc gggtatcgaa cgcgcgtacc actgcgccga gcgagtttgc cgtaccgatc
     3541 gccgccaaac ctgccacacc tgcaccaatc accaaaacct gcgcgggcgg cactttgccg
     3601 gcggcggtaa tttgaccggt gaagaaacgg ccgaaggcgt tggcggcttc aattacggcg
     3661 cggtagccgc tgatgtttgc catcgaagac aaagcgtcca aagcctgcgc gcgcgaaatg
     3721 cggggcacca tatccatcgc cagcgcgttc actttcttgg cgcgcaaggc ttcgaccaaa
     3781 gcctcgtttt ggcgcggcca caggaagctg acgatggttt gaccttcgtt caaaagcggc
     3841 agttcctgtt cggacggcgc gttgacctta taaatcaaag ggcagaccca aaccgccgct
     3901 ttgtcggcaa cggttgcgcc tgctgtttgg taagcggcat cgtccaaact tgccgccaaa
     3961 cctgcaccgc tttcgacaac ggtttcaaag cccagtttgc ccagcagggc gacggtggcg
     4021 ggcgtgcagg cgacgcgggt ttcgccggat aatgactcgc gtgggatacc gattttcatc
     4081 tctgaatcct ttttcgggta gtttatatgt atcgtgggtt aaatttaaat cggggcaggg
     4141 cggagcaacg ccgtaccggt ttaaaaccga ttcacttcaa atgttaatat attttagata
     4201 atccccttat aacgaatttt catcaggctg gcaatagttg cggcattttc ccgtgttgtc
     4261 cgacacatac tgccgtttgc gtttgaagtc attttccgat tgcagtccct cccgccgtaa
     4321 cattctttgc cgtttcacta tataatccgc attttttgag ccgccgttat gccgcacgcc
     4381 ctcgtcctcc aatttccctc cgccgaagcc ctgccttccg acttcccctc ccgcctgccc
     4441 gaacctgatt gcgccgatga aaagcgtatg cgttttatcg ttgaagaagg gttttcttta
     4501 agcggggcgg acgcggcgtt gcttggcagc cgtcaaatcg accacgccgt gttgccggat
     4561 atggattttg gcgaactcgg tttgattgtc agcgatatgg attcgacgct gattaccatc
     4621 gaatgcgtcg atgaaattgc ggcaggcgtg ggtttaaaag acaaggtagc ggaaattacc
     4681 gagcgttcga tgcgcggaga gttggatttc gagcagtcgt tgcgcagccg cgttgcgctg
     4741 ttggcgggat tggacgaaca gattttggcg gacgtttatg aaaacgtttt gaagctctcg
     4801 cccggtgcgg aatttttgtt ggacgaatgc aaaaggcacg atgtgaaatt cctgctggtg
     4861 tccggcgggt tcacgttttt taccgaacgt ctgcaacaac gcctcggctt cgaataccaa
     4921 cacgccaatg ttttggaaat tgaaaacggc aggttgaccg gccgtctgaa aggcagaatc
     4981 atcgacgcgc aggcaaaggc ggatttattg cgcgaatacc gcagccgcct cggattgcag
     5041 ccacatcagg tgttggcgat gggcgacggt gcgaacgata ttccgatgct caaagaagcg
     5101 ggcataggcg tggcttaccg tgccaaaccg aaagcgcggg ccgccgccga tgcctgtatc
     5161 aatttcggcg gtttggagcg tgtacgcggc ctgttcggat aggcggatag gaaacggatg
     5221 tcgtccgaaa ggttttcaga cggcatttga acggcaggaa cgacagtggg acgcagaaaa
     5281 ctttggtttg ccctggcagc agcaggcgtt atcggcggtt tggtcggcat tgtgctgacg
     5341 gaactgatgc acttcataca gcatacggca tacggttatg gcgcggacgg tgcgtacatc
     5401 tcgttccgcg aaggtgtggc ccgtgcttcg gaagggcggc gtttaggcgt attgattctg
     5461 tgcggtatgt tggcaggcgg cggctggtgg ttgctcaaac gtttcggtaa gccgcaaatc
     5521 gaaatcaaag ccgccttgaa gcagccgttg caggggctgc cgtttctgac gacggttttc
     5581 catgttctgc tgcaaatcat aacggtcgga ctcggttcac cgctcggacg ggaagtcgcc
     5641 ccgcgtgaaa tgactgccgc gttcgctttt gccggcggca ggcggttggg tttggatgaa
     5701 gacgaaatgc ggctgctgat tgcttgcgcc tcgggtgcag gtttggcggc cgtgtataac
     5761 gtaccgctcg cctccacact tttcattctc gaagccatgc tgggcgtgtg gacgcagcaa
     5821 gccgtcgccg ctgcattgtt aacttcagtc atcgccaccg ccgtcgcgcg catcggcttg
     5881 ggcgacgtgc agcaatatca tccggccaac cttaccgtca atacttcatt actttggttt
     5941 tccgccgtca tcggcccgat actgggcgta gccgccgtct ttttccagcg taccgcccaa
     6001 aagttcccct ttatcaagcg cgacaatatc aaaattattc ccttggccgt ctgtatgttt
     6061 gcactcatcg gcgtgatttc cgtttggttt cccgaaattt tgggcaatgg caaagcaggc
     6121 aatcaactga cctttggcgg attgaccgat tggcaacaca gccttgggct gaccgccgtc
     6181 aaatggctgg tcgtcttaat ggcgcttgcc gtcggcgcat acggcggtct gattaccccg
     6241 tccatgatgc tcggcagtac catcgccttt gctgctgcca ccgcgtggaa cagtgttttt
     6301 cctgaaatgt cctctgaaag cgcagccatt gtcggcgccg cagttttcct cggtgtttcc
     6361 cttaaaatgc ccctgaccgc catagccttt attttggagc tcacctacgc ccctgttgcc
     6421 ttgctcatgc cattatgtac aggcatggca ggtgcagtat gggtggcaaa gaaaatggga
     6481 tttaaatagg caaaagcaaa aggccgtctg aaaccaagtt tcagacggcc ttttacaata
     6541 aaattgttaa caatatttgc aaaaacctac tgccaaaaat gcgaaactgg gggataatac
     6601 cgccctgaaa attcatccca tactgattaa accttcaaca aaggaaatcc aaatgtcttc
     6661 catcaaacgc gccctgatca gcctatccga caagacaggc gcagtcgaat ttgcccaaac
     6721 cctgcacaaa ctcggtgtcg aaattctttc taccggcggt acagcaaaac tcttggctga
     6781 tgcaggcgtt cccgttatcg aagttgccga ctataccggt tttcccgaaa tgctcgacgg
     6841 ccgcgtgaaa accctgcatc cgaaaatcca cggcggtatt ctcggtcgtc gcgatttgga
     6901 cgaacacgtc gccaagatgg aagaacacgg catcggcaat atcgacctcg tgtgcgtcaa
     6961 cctctacccc ttcgctgcca ccatcgccaa accaaactgc acgctggaag acgcgattga
     7021 aaacatcgac atcggcggcc cgaccatggt gcgctctgcc gcgaaaaact ggaaacacgt
     7081 cgccatcgtt accgacaccg ccgatttccc ggccatagct gccgaactcg aagccaacaa
     7141 cggcgcattg agcgacaaaa cccgtttcaa cctctcgcgc aaagcattca gccataccgc
     7201 ccaatacgac ggtatgattt ccaattacct gacctcgctt tcagacgacg tcttgagcgg
     7261 cacgcccgaa atcgccggat tccccggccg gttcaatcaa agctggatta aagtgcaaga
     7321 catgcgctac ggcgaaaacc cgcatcagcg cgccgcgttc taccgcgata ttgaccccgc
     7381 cgcaggcagc ctcgctgcat acaaacaact gcaaggcaaa gaattgtctt acaacaacat
     7441 cgccgatgcc gatgccgcat gggaagccgt caaatccttc gacgtgcccg cctgcgtgat
     7501 tgtgaaacac gccaatccgt gcggcgtagc catcgcctcc aataccttgg atgcctacaa
     7561 actcgcctac gccaccgaca ccaccagcgc gttcggcggc atcatcgctt tcaaccgcga
     7621 agttgacggc gcaaccgtca aacaaattac cgacaaccag tttatggaag tcctcatggc
     7681 gcctaagttc accgccgaag ccctcgaaat cgccgccgcc aagaaaaacg tgcgcgtatt
     7741 ggaagtgccg cttgaggcag gcgcaaaccg cttcgaactc aaacgcgtcg gcggcggact
     7801 gttggtgcaa acgcccgaca tccaccgcat cagccgcgcc gatttgaaag tcgtctccaa
     7861 acgccaaccg accgagcagg aatggaacga tttgctgttc gtctggaacg tcgccaaata
     7921 cgtcaaatcc aacgccatcg tattcggcaa aggcggtcaa acctacggca tcggcgcagg
     7981 ccaaatgagc cgcgtggaca gcacccgcat cgccgcccgc aaagcgcaag atgccggtct
     8041 cgacctcaac ggcgcgtgtg ccgcatccga tgccttcttc cccttccgcg acggcgtgga
     8101 cgtgattgcc gaacagggca tcaaagccat catccatccg gcaggctcga tgcgcgatca
     8161 ggaagttttc gacgcagccg acgaacacgg catcgccatg gtcgtaaccg gcatccgcca
     8221 tttccgccat tgatgcagat aaacaaggta atgccgtctg aagggctttc agacggtatt
     8281 ttgcgctatt ttgcgaaggt agggatgacg gttcgggtat tcctgacagg gtggattttc
     8341 aaggtgttgt atagggtgta ggaggattcg taaaaggtgg gatgcagggt gtgcttcagc
     8401 ccgctgcatc aaaaattttt ggagaaccgg cgggagtcgg cggttttggt ttcggcgggg
     8461 acggtggaaa tgggtaacat tgacggaatc gacggaagcg gtgggctgaa gcccaccctt
     8521 gtatattgga atcactgtat catagcaaca aaccgcccga ccgccacccg cgcccaccca
     8581 aggcacacaa ccgttgcgta gcacagggag cggcagggca acccatcgac acaaccggac
     8641 agttgcccga caacacaacc gaatgcaagg caggttgatg atgagtaccc
//
LOCUS       DAWHRA010000087         8502 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWHRA010000087 DAWHRA010000000
VERSION     DAWHRA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.087"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..121)
                     /locus_tag="WIB30_001462"
     CDS             complement(<1..121)
                     /locus_tag="WIB30_001462"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ3797516.1"
                     /translation="MPSETTKQRFRRHPFQKRTIMKNIVILISGRGSNMQAIVN"
     gene            complement(168..986)
                     /locus_tag="WIB30_001463"
     CDS             complement(168..986)
                     /locus_tag="WIB30_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3797517.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(1245..1685)
                     /locus_tag="WIB30_001464"
     CDS             complement(1245..1685)
                     /locus_tag="WIB30_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ3797518.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEELADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(1748..3154)
                     /locus_tag="WIB30_001465"
     CDS             complement(1748..3154)
                     /locus_tag="WIB30_001465"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ3797519.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEASQLSNPTVLALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDVHVFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHTAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            3325..4440
                     /gene="lptF"
                     /locus_tag="WIB30_001466"
     CDS             3325..4440
                     /gene="lptF"
                     /locus_tag="WIB30_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ3797520.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFAVALILLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            4437..5507
                     /gene="lptG"
                     /locus_tag="WIB30_001467"
     CDS             4437..5507
                     /gene="lptG"
                     /locus_tag="WIB30_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ3797521.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            5717..8302
                     /gene="acnB"
                     /locus_tag="WIB30_001468"
     CDS             5717..8302
                     /gene="acnB"
                     /locus_tag="WIB30_001468"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ3797522.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLASIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAIEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
BASE COUNT         1914 a         2394 c         2209 g         1985 t
ORIGIN      
        1 cattgacgat tgcctgcata ttgctgccgc gtccagaaat caggatgacg atgtttttca
       61 taatggtgcg cttttgaaag ggatgccgtc tgaaccgctg tttggtggtt tcagacggca
      121 tttgccgtaa aaatgcccga aaacctgttt cgggcatgga ttcggactta atttactttt
      181 ttgatgtcga cttgagccgg ctgcttggcg ggcgcgtttt cgggtgcgcc gattttgacc
      241 agtttcacat caaataccaa agtggcgttc ggaccgattt tgtcgcccgc accctgttcg
      301 cggtaggcaa ggttggacgg gatgtagaac gtggcttcgc cgccttcttt cagaagctgt
      361 acgccttcgg tccaacccgg aatcacttgg ctcaaaggga aggtgaccgg gccgccgttg
      421 gctttgctgc tgtcgaatac cgtaccgtca atcaggcggc cttcgtattc cacggtaacg
      481 atgtcgtctt tggtcggctg tttgccttcg ccctgtttgg tgattttgta ttgcaggccg
      541 gaagcagtgg tcttcacgcc gtctttggcg gcattttctt tcagaaaggc ttcgcctttt
      601 tctttattgg ccttcgcgtc cgccttgtgt ttttctacgg ctttagcctg ttgttcctga
      661 aggaatttca tcatgacttc ctgagcctgc tcttcggtca ttttgatttc tttgccgtca
      721 tacactgcct gcatggcttc ggtaaagact ttcaaatcga tttccgcgcc ctgttccttc
      781 atttgcttca gggagcgtcc gatgtccacg cccatcgcat agcttgcctg ctgcatcgtg
      841 ctgccgatcg aagaggtgtc gccctgcgcg gaagaagcgg cggcaggttc ggatgcagat
      901 gcgggggcgg cttctttttt gccgcaggcg gaaagtgcca aagcggcgga aagggtcagt
      961 gcgctgattt tgaaaatggt gttcatgatg gatcttcgct gtcgataagg tcggaaaaac
     1021 gggattatag ccgagtttga atgtttcaac acacaggatg acacataaag cgtcaatcgt
     1081 gtgttgccct gttttggaag ggattgaacc ttccaaaata agttttgatt ctaccgcccc
     1141 gagggacaga tgtccaagtg gcggggttca accgataagg aaattttaat caaatagaat
     1201 caagcctgtt taaattttgt aaatgcggca tttcagacgg cattttatgc cttgccctcc
     1261 atgccgtgat gttcgatggc aaaaccgctt cggcggtagg cggtaaagcg ttcgcgcgcg
     1321 tcggcgagct cttccaagct gttgccgacg atttccaaaa cgcgcgcggg caagaccgga
     1381 gcggtgttcc aaaaaccgtc ggacaggttc aaaacggtca tgccttcggg aattcggggc
     1441 agattgccgc cgcaggcaag caggacggat ggttcagacg gcatggcttc ttccgtttcc
     1501 caaatttcgt gcggaatgaa actctcggcc tcgtattgcc aaagcatttt gtccaattcc
     1561 tgaagctgcc cgaacgaatc ggaccacacc agtatcctgc cgccgtcccg aatcgcacgg
     1621 gcaatcaggc ggcaggtgaa aatcggaact tgggcaacgt gcgtgtaaaa ggtggctttc
     1681 ggcatattgt ttgaacattt ggcaggataa tgccgtctga aaggcttcag acggcattgt
     1741 ggcaaaatta aagattccgc agatagttca gcagcaaggg aacgggacgg ccggtcgcac
     1801 ctttttccgc accggatttc cacgccgtac ccgcgatgtc aaggtgtgcc catggatagt
     1861 cttcggtaaa gtaggatagg aatgttgcgg cggtaatcgt gcccgcgccg ggcgtgccga
     1921 tgtttggaat gtcggcaaag ttggatttga gttggtcttt gtaggtctca aagagcggca
     1981 gttgccatgc tttgtcgtcc acgttgtagg aagcggcaag caggctgtcg atcaaatcct
     2041 gattgttgcc catcacgccg ctgacatcgt gccccaaggc aacaatacac gcgccggtca
     2101 gggtggcgac gtcgatgacg gctttgggtt tgaactgctc ggcgtaagtg agcgcgtcgc
     2161 acaaaatcag acggccttcg gcatcggtgt tcaacacttc gatggtcagc cctttcatac
     2221 ttttcacgac atcgcccggt ttgtttgccg cgccggaagg catattttca caagtggcga
     2281 cgacggcaat caggttaatc ggcagttgca gtttgacggc ggcgcagaag gtgctgatga
     2341 cggttgccgc gccgcacata tcaaacttca tttcgtccat gttcaggccg ggcttgaggg
     2401 agatgccgcc ggtgtcgaag gtaatgcctt tgccgaccaa taccacaggc gcggcttctt
     2461 tgtcggctgc accgaaatag ctcagttcga ccaaataggg ggcttccgcg ctgcctttgg
     2521 cgaccgacca aaacgaaccc atgttttctt tgatgtagtc tttttcgatg attttggcgt
     2581 gcgcgcccag tttttcggct tcggctttgg cggtgcgcgc taaaaattcg ggcgtgcatt
     2641 cgttgggcgc ggcgttgccc aagtcgcggc agaggctttg tccgtaaact tgcgcttcgg
     2701 cgacgcgcaa ggcttctttg acggcggctt cgtgcgcggt atggaacacg gcagtttcaa
     2761 atttggcggg cttggcttct tttttgtagc ggtcgaaacg gtaggcggca ttgccgaacg
     2821 caatcgcaaa cgcttcggca acggctgcag cctgcgcttc ttcaaagacg tgaacgtcca
     2881 cattgaccgt ttcctgattt tgcgcccatt tggcggcttc ggcggcggcc ttgttcaatg
     2941 cggcgcggcc ggtgcttttc agacagcata cggcaacagc ctgcaaaccg ttgcctgtcg
     3001 ggatttttgt gtcggcaaaa ttttgacctt cttcaagcga agacaaaagg gcaaggacgg
     3061 tcgggttgct cagttgcgat gcttcggtgc agacaaataa ctgtgcgcct gcctgctgtt
     3121 cctgcaagat ttcggttttt gtgctaaatt ccacgtttat tctcctgatt gagacggttg
     3181 tcggtagttt tcggacggcc tttcgctcaa aagaccgtct gaagacggct ggcacgattg
     3241 taccccattt gaagcaccgt ctgaaacctt gcgcggacaa tccgcctgcg ccgaaccgct
     3301 taccgccccc ctgaccgcga ttctatgatt tatcaaagaa acctcatcaa agaactctct
     3361 tttaccgccg tcggcatttt cgtcgtcctc ttggcggtat tggtctccac gcaggcaatc
     3421 aacctgctcg gccgtgccgc cgacgggcgt gtcgccatcg atgccgtgtt ggcattggtc
     3481 ggcttctggg tcatcggtat gacgccgctt ttgctggtgt tgaccgcatt tatcagtacg
     3541 ttgaccgtgt tgacccgcta ctggcgcgac agcgaaatgt cggtctggct atcctgcgga
     3601 ttggcattga aacaatggat acgcccggtg atgcagtttg ccgtgccgtt tgccgttttg
     3661 gttgccgtca tgcagctttg ggtgataccg tgggcagagc tacgcagccg cgaatacgct
     3721 gaaatcctga agcagaagca ggaattgtct ttggtggagg caggcgagtt caacagtttg
     3781 ggcaagcgca acggcagggt ttattttgtc gaaaccttcg ataccgaatc cggcatcatg
     3841 aaaaacctgt tcctgcgcga acaggacaaa aacggcggcg acaacatcat cttcgccaaa
     3901 gaaggtaact tctcgctgaa cgacaacaaa cgcacgctcg aattgcgcca cggctaccgt
     3961 tacagcggca cgcccggacg cgccgactac aatcaggttt ccttccaaaa actcaacctg
     4021 attatcagca ccacgcccaa actcatcgac cccgtttccc accgccgtac cattccgacc
     4081 gcccaactga ttggcagcag caacccgcaa catcaggcgg aattgatgtg gcgcatctcg
     4141 ctgaccgtca gcgtcctcct actctgcctg cttgccgtgc cgctttccta tttcaacccg
     4201 cgcagcggac atacctacaa tatcttgatt gccatcggtt tgtttttaat ttaccaaaac
     4261 gggctgaccc tgctttttga agccgtggaa gacggcaaaa tccatttttg gctcggactg
     4321 ctgcctatgc acattatcat gtttgccgtt gcactcatcc tgttgcgcgt ccgcagtatg
     4381 cccagccagc ccttctggca ggcggttggc aaaagtctga cattgaaagg cggaaaatga
     4441 acctgatttc acgttacatc atccgtcaaa tggcggttat ggcggtttac gcgctccttg
     4501 ccttcctcgc tttgtacagc ttttttgaaa tcctgtacga aaccggcaac ctcggcaaag
     4561 gcagttacgg catatgggaa atgctgggct acaccgccct caaaatgccc gcccgcgcct
     4621 acgaactgat tcccctcgcc gtccttatcg gcggactggt ctccctcagc cagcttgccg
     4681 ccggcagcga actgaccgtc atcaaagcca gcggcatgag caccaaaaag ctgctgttga
     4741 ttctgtcgca gttcggtttt atttttgcta ttgccaccgt cgcgctcggc gaatgggttg
     4801 cgcccacact gagccaaaaa gccgaaaaca tcaaagccgc cgccatcaac ggcaaaatca
     4861 gcaccggcaa taccggcctt tggctgaaag aaaaaaacag cattatcaat gtgcgcgaaa
     4921 tgttgcccga ccatacgctt ttgggcatca aaatttgggc gcgcaacgat aaaaacgaat
     4981 tggcagaggc agtggaagcc gattccgccg ttttgaacag cgacggcagt tggcagttga
     5041 aaaacatccg ccgcagcacg cttggcgaag acaaagtcga ggtctctatt gcggctgaag
     5101 aaaactggcc gatttccgtc aaacgcaacc tgatggacgt attgctcgtc aaacccgacc
     5161 aaatgtccgt cggcgaactg accacctaca tccgccacct ccaaaacaac agccaaaaca
     5221 cccgaatcta cgccatcgca tggtggcgca aattggttta ccccgccgca gcctgggtga
     5281 tggcgctcgt cgcctttgcc tttaccccgc aaaccacccg ccacggcaat atgggcttaa
     5341 aactcttcgg cggcatctgt ctcggattgc tgttccacct tgccggacgg ctcttcgggt
     5401 ttaccagcca actctacggc atcccgccct tcctcgccgg cgcactacct accatagcct
     5461 tcgccttgct cgccgtttgg ctgatacgca aacaggaaaa acgttgaacc aatgccgtct
     5521 gaacctctct tcagacggca tttgttttca ttgacacatt cccacagaca gatagccgtt
     5581 ccctattaca ttacctgtca taacagttcc atttttgtta aaactagtct atgatagcgg
     5641 tacaaatatt gtttacaata tttaacgcaa atcatttgca acccgacaaa agaaaaacag
     5701 aaaaaggaac aaagagatgt tagaagccta tcgtaaagcc gccgccgagc gcgccgccct
     5761 cggcattccc gccctccctt tgaacgcgca gcaaaccgcc gatttggttg agctgctgaa
     5821 aagcccgccc gcaggcgaag gcgagttctt ggtcgaactg cttgcccacc gtgttccgcc
     5881 cggtgtggac gatgccgcca aagtcaaagc ctcattcctg gctgccgttg ccgaaggcag
     5941 cgcgtccagc ccgctgatct cccccgaata tgcgaccgaa ctcttaggta caatgctcgg
     6001 cggttacaat attcacgcct taatcgaact cttggacgac gacaaactcg cgtccattgc
     6061 tgccaaaggc ttgaaacata cgcttctgat gttcgattcc ttccacgacg ttcaagaaaa
     6121 agccgaaaaa ggcaacaaat acgcgcaaga agttttgcaa tcttgggcag atgccgaatg
     6181 gttcgcctca cgcgccaaag ttcccgaaaa aatcaccgtt accgttttca aagttgacgg
     6241 cgaaaccaat acagacgacc tctcccccgc gcccgacgcg tggagtcgtc ccgatattcc
     6301 gctgcacgcg ctggccatgc tgaaaaaccc gcgcgacggc atcacgcccg acaaaccggg
     6361 cgaagtcggt ccgattaaat tgttggaaga actcaaagcc aaaggccatc cggttgctta
     6421 cgtcggcgac gtggtcggta ctggttcttc acgcaaatcc gcgaccaact ccgtcatttg
     6481 gcataccggc gaagacattc cgttcgtgcc gaacaaacgc ttcggcggcg tatgtttggg
     6541 cggcaaaatc gcgccgattt tcttcaatac ccaagaagat tccggcgcgc tgccgattga
     6601 agtcgatgta tctgctctaa aaatgggcga tgtcgtcgat atcctgcctt atgaaggcaa
     6661 aatcgtgaaa aacggcgaga ctgttgccga gtttgaattg aaatcacaag tattgctgga
     6721 cgaagtgcaa gccggcggcc gtatcaacct gattatcggc cgaggtctga ccgccaaagc
     6781 gcgcgaagcc ctgaaactgc ctgcctctac tgcattccgc ctgccgcaag cgcctgccga
     6841 aagcaaagcc ggtttcacct tggcgcaaaa aatggtcggc cgcgcctgcg gtctgcccga
     6901 aggacaaggc gtgcgcccgg gtacttactg cgaaccgcgt atgacgacgg tcggctcgca
     6961 agacacgacc ggcccgatga cccgcgacga gttgaaagac ttggcttgtt tgggcttctc
     7021 cgccgatatg gtgatgcagt ctttctgcca caccgccgcc tatccgaaac ctgtcgatgt
     7081 aaaaacccat aaagaactgc ccgcctttat ttccacccgt ggcggcgtgt cactgcgtcc
     7141 gggcgacggc gtcatccact cgtggctcaa ccgcctgctg ctgcccgata ccgtcggcac
     7201 cggcggcgac agccataccc gtttccccat cggtatttcc ttccccgccg gctccggctt
     7261 ggttgccttt gccgccgcaa cgggcgtaat gccgctcgat atgcccgagt ctgtattggt
     7321 acgcttcagc ggcaagctgc aaccgggcgt aaccctgcgc gatttggtga acgccatccc
     7381 gctgtacgca atcaaacaag gtttgctgac cgttgccaaa gccggtaaga aaaacatctt
     7441 ctccggccgc atcctcgaaa tcgaaggcct gcctgatttg aaagtggaac aagcctttga
     7501 attgaccgac gcatccgccg aacgctccgc cgccggctgt accgtgaagc tcaacaaaga
     7561 gccgattatc gagtacatga aatccaacgt cgtgttgatg aaaaacatga ttgccaacgg
     7621 ctatcaagac ccgcgcactt tggaacgccg catcaaagct atagaaaaat ggctggcaaa
     7681 tcctgagttg ctcgaagcgg ataaagatgc cgaatacgcc gccgtgattg aaatcaacat
     7741 ggacgacatc aaagagccga ttatcgcctg cccgaacgac ccggacgacg tgtgcttcat
     7801 gtccgaacgc tccggcacca aaatcgacga agtattcatc ggttcgtgta tgaccaacat
     7861 cggccacttc cgcgccgcct ccaaactttt ggaaggcaag gcagacaccc ccgtccgcct
     7921 gtggattgcg ccgccgacca aaatggacgc gaaacaattg tccgacgaag gacactacgg
     7981 cgtactcgga cgtgccggcg cgcgtatgga aatgccgggt tgctccttat gtatgggtaa
     8041 tcaggcgcaa gtacgcgaag gtgcgaccgt tatgtccacc tccacccgca acttcccgaa
     8101 ccgtttgggt aaaaacacct ttgtttacct cggttcggcg gaattggcag cgatttgctc
     8161 caaactgggt aaaatcccga ccgttgaaga atatcaagcc aatatcggca tcatcaacga
     8221 acagggcgat aaaatctacc gctatatgaa cttcaacgaa atcgacagct acaacgaagt
     8281 agccgagacc gtgaacgttt aatccccgtc atccgtatga agtaagggat tgaccgcaat
     8341 gccgtctgaa caaccttcag acggcattgc aacattccgc taacccttct ttccgcaaac
     8401 gctgcaaata cggcgttcac gcccccacat aaaggaaacg acagtgaacc tgaaaaaccg
     8461 ccattttctg aaacttttag acttcacgcc ggaagaaatc ac
//
LOCUS       DAWHRA010000088         8319 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWHRA010000088 DAWHRA010000000
VERSION     DAWHRA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.088"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(144..539)
                     /locus_tag="WIB30_001469"
     CDS             complement(144..539)
                     /locus_tag="WIB30_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797523.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            complement(523..840)
                     /locus_tag="WIB30_001470"
     CDS             complement(523..840)
                     /locus_tag="WIB30_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ3797524.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
     gene            complement(968..1246)
                     /locus_tag="WIB30_001471"
     CDS             complement(968..1246)
                     /locus_tag="WIB30_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797525.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(1253..1537)
                     /locus_tag="WIB30_001472"
     CDS             complement(1253..1537)
                     /locus_tag="WIB30_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797526.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGIFAALFVFSIVKRVMK"
     gene            complement(1545..1742)
                     /locus_tag="WIB30_001473"
     CDS             complement(1545..1742)
                     /locus_tag="WIB30_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797527.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(1747..2037)
                     /locus_tag="WIB30_001474"
     CDS             complement(1747..2037)
                     /locus_tag="WIB30_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797528.1"
                     /translation="MNIQLQGHIVGVKKINGQIEGKSFDYCCLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            complement(2082..3424)
                     /locus_tag="WIB30_001475"
                     /pseudo
     CDS             complement(2082..3424)
                     /locus_tag="WIB30_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            complement(3495..3740)
                     /locus_tag="WIB30_001476"
     CDS             complement(3495..3740)
                     /locus_tag="WIB30_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797529.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(4029..4382)
                     /locus_tag="WIB30_001477"
     CDS             complement(4029..4382)
                     /locus_tag="WIB30_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797530.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            4618..6045
                     /locus_tag="WIB30_001478"
     CDS             4618..6045
                     /locus_tag="WIB30_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ3797531.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            6114..7517
                     /locus_tag="WIB30_001479"
     CDS             6114..7517
                     /locus_tag="WIB30_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ3797532.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(7681..8274)
                     /locus_tag="WIB30_001480"
     CDS             complement(7681..8274)
                     /locus_tag="WIB30_001480"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ3797533.1"
                     /translation="MYGSHRLLCTQTDGDNAQKKTTTYKEQDPAKVTHYLTQPAEFSD
                     YQRVYLDETGFDRHLFRPYARSLKGQIVKAQISGKRYRRLSLVSAQVGNRLIAPMVYQ
                     NTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHKVLPLAPYSP
                     ELNPIEKVWANIKRYLRTVLSDYARFNDALLSYFDFN"
BASE COUNT         2094 a         1982 c         2026 g         2217 t
ORIGIN      
        1 cccgacagcc gaaacccaaa cacaggtttt cgtctatttc cgctaccaat cactccctaa
       61 ttctacccaa ataccccctt aatcctcccc ggatacccga taatcaggca tccggggtac
      121 cttttaggcg gcaacaggcg cacttagcct gagacctttg caaatttgtc ggtttcgggg
      181 tcgtattggt agcctcgtgc ctgtatgtct tctttgaaag tttcgtatac gtcgtgggct
      241 aaaagggctg ttccgacata gggaaccgcc cttgtgctga atttcgcgcc taagcgggca
      301 agtttgccga cccccgccaa tacgccggcg cgggatacgc tggcggttat tttggcgttg
      361 attcgggctt ttgcgcccgt agggatgtgt gttaaatcta ccgtttttat taaatcagat
      421 gaataagttt tactattttt aggtacaaac ttatgaattt tcgcaccttg tccggtatca
      481 actgaaacag tttcagatat ttttactgca tttgcattcg cttcaaacga atacatcatc
      541 aaaattgcaa ttatcgacaa tttcgcaaaa ttcaaatttg tatattttat gaccatcttt
      601 cagagattct ttaattacca tttctgaatt atcagaaaat gagattagcc aaatatcatg
      661 tttaattctt ctattccaga aaaaagagaa acaatcaata acattttcag acttattaat
      721 cttcgcaaat tcaacaaatt cagattgcgc tataaccgcc atcgattgcc caaaatactt
      781 gcttgacggc tgatatttat aaagtgccaa ctgcgcctga gtgataaacg gcttgttcat
      841 ggttctgcct ttcaaaggtt gttttgaaag cctgattttg acaccataac ttcatgcgct
      901 caattcttaa acagaaccgc cccgattaat acgggtacgg aaacgccgag ataaaaataa
      961 aaatccatca tttcaaaacc tttttcagca gggaaacaaa gtaaacggac gcgaggatgc
     1021 cgaatactat ccagcctgtt tcaagaccgc tttgcaggtt gtctttcgga ctgcattccg
     1081 ccaataaaag ccttagcggc tgaccgtccg acatcttcca caggctgccg ttatattccg
     1141 gcctgacaat ctgtccgttt tctttgattc ttggtactac caagctgaaa taaaggtttt
     1201 cagcctggtg cttctcaaga catttatttc cgacttggta gtacatgccg tcttacttca
     1261 tcactctctt aacgatggaa aatacaaaaa gcgcggcgaa aatgcccact acaatccaac
     1321 cggcttccat accgtccgct tttgcggctt ccaaagcgtt ttttgccgta tcgggcaacg
     1381 ttgcatttgc atgtgcggcc aaagccaggg gagcagctgt tacaacagcc agttttgcgc
     1441 cgtatttacg gcaggtgtta ataaatttca tgatattttc cttcaaaaag tgtttggcgg
     1501 taatggatgg agcgtttttc agacgaccgc cgaacatccg aaaatcagtc tttcaaaaat
     1561 ccgaatacga caaattcgta ttggttgccg atttcttcca aacctgcgtt aatcgcttct
     1621 tcgaagtcgt agaaataatc ggcattggtg attaatttgg tatgtccgat gtcgcccgtt
     1681 tcaggagaga gatacagaaa gtcccctgtt gatacggact ggacaacata gactttctgc
     1741 attcaatcag cctttcttca cgagttgaaa accgatgact ttcagttttt gggttttgcc
     1801 cgtagtgacg atttctacgt tcaggtttgc ttcgatcgga aattgggcgt ttcggaactg
     1861 ctcgaaattg gcagagccgc cgaaatcgta ttcagtagta gagctgccca atgcgttgcc
     1921 ttgggagctg tctaagggtg tggcgacaat caggcagcaa tagtcgaagc tcttgccttc
     1981 gatttgtccg ttgatttttt taacgccgac gatgtggcct tgaagttgga tgttcatttt
     2041 ttggtttcct tgtgtgatta aacgtctttc gggcagacac tttaagccca tgaaatcggt
     2101 agtcttgcga atttgtcgta aatgaagttg ttatagcttt cttcattgtt gacgtgtttt
     2161 tgctgttcaa gctgtttttc aagattctcg taatattcgt acatatagta agggtctttg
     2221 tacggtttga atgcgggctg ttcatgaatg gcttgagctt tcaaaaaggc gcagtcgtag
     2281 gcttcgggag ccaaagactt gggcagcttg tgatgactcg gctcaatcag ttcaaacagt
     2341 ttggctttgt ccaattcggg aaaaatgaat ttcagaccgt ttgccgcacg tccgaactgt
     2401 ttttttaccc attcaaggta gcggtcggct gaaatgacct tatcttcctt aaccgcgtgt
     2461 atgcgcgttg ccttttgggc gaatcgttcg caaatcggat atgcgccgcc gaaatattcg
     2521 cccggattct gcaaaacttc gaaagggata acgatgtctt ttgctttgaa ttcaatttca
     2581 aatcgcgtcc atgtgcttgt tttatcgccc aactgcttgc ctttttcata gacgcggaca
     2641 tatttggacg attcacggga gccgatacca taggtcttgc ctttggtcat tttggcttca
     2701 tcgtcttctt cccaatctga ccccaaacat tcgccttttg gtttgacgtg atgacaggta
     2761 aacatacctt tatttcggtc ttcacgggct tggttcgggc tgtattcgcc gttgaaaaag
     2821 tcttttgcga tgtcaacgcg tgtgattttt gggcggattg cattagtcag gaatgcgaaa
     2881 agtcgtgatt cccagccttc ttttgcgacg ccgcaaccgg tgccggtcag ttcgaaaaga
     2941 atggtatttt gttggccgcc aaaatggacg cgaccgtata gggcgtcttc cgaacccatc
     3001 aaccaacagc gctcatagaa acgaccgccc gagcctttgg attctttgta gataccgaaa
     3061 ccgaaaactt cttcggcgag catggacgcg gcgcgaataa aatcttcgtc ttccaaaaga
     3121 cttacacgaa cgccgtattt atcgaaaaag gttttttcat gaaatgaaaa gctaatttga
     3181 tcaatgaaag ccgaatctga tacaccgcgc cgaagaggaa cgcctaacag gtttccttta
     3241 ccgtccgtta tgtacgtttc gtaacattcg aagacttcct gaaccctgcc tgccgtttcg
     3301 gtttctgtcc ccccctgtta gataaggggg gaagatttga agcggttgtc ggcttcctgc
     3361 cgtccgctag cgcgtccgtc atcacgccgg caaccgcctt tgtcatccct tgcttatctt
     3421 ccatggtgcg aatcctcaaa aacgggcaaa aaaaagccct gttacttgta gaaagtaaag
     3481 gacgttaatt tttgttaatc gtcccttctt agggacgcaa tatataaggg acgcaatata
     3541 taaggtttta aaacgctaag aacaaaatcc gataacggct ctgaagaagt aagtctttct
     3601 ttattgcaca agatagcaaa cttccacggc attctcgccc cacccgtgcc gtccaagcct
     3661 ttttcaaaga catagatggt tttcttggca atcacttcgt cagtttggcg gtcgataatc
     3721 cgtatggtcg cacctgccac ccagtgcctg cgcagcttgg aatcgacatt gttttcgaac
     3781 gttaccgcat aacgggcggg gtgtatatga ttaagtattt gatttagcgg aaaaatcttt
     3841 acctgaatac cgaataatat cggaatggtt gggttgcagg acatccacat agcggtattc
     3901 cccatcgcta aaaaatccta ggaaacgagc aataaaattt acgccttcgc tggtctgtaa
     3961 agccgcattg tcccacatcg ggtctctggt ttttgcatct gccgaaacgg tacgctcagg
     4021 tactaccttc aacagcataa tcccttccac attgtccgcc gtctggtaaa tctttccccc
     4081 gccgttttgc attgttcgtt aaacacggct tcggcttctt tgtattttct gtcccactct
     4141 tcttgtgcct gtatttcttc tttgatcggg ccgaattgtt tgggaataat ccaaacaaac
     4201 agcatcagga tagcggcggc ggtcaggctg cctgaaagga ttttgccggg gttccgtttg
     4261 ggctttttat aggcaaagcg gacgagaaac caaagcaaca gcagcatggt gccccaatag
     4321 ccgattgaga ataggatggc caaaccttct aggaaatggc gtaaatcgtt tgtggtaaac
     4381 atgggttgtt cctgtggtta aatgtgcagg ctgctttttg ccgaaccttg ccgcatctca
     4441 aaagcagcct gcgcttcagc gttgcgttac gcagtaaaat aatgaatatt tgtaacgact
     4501 tgggtatttt ttgtcaatat tcccgccttt cccttaacag ctgccgcgct ttccgttaaa
     4561 attcctttac atatttatat tgtttcctgt ttctatattg ccaaggttat acccgttatg
     4621 tttttctccg ccctgaaatc ctttctttct cgatacatta ctgtatggcg caatgtttgg
     4681 gcggtgcgcg accagttgaa accgcccaaa cgcacggcgg aagaacaggc gtttttgccc
     4741 gcgcatttgg aactgaccga tacgccggtc tctgccgctc cgaaatgggc ggcgcgtttt
     4801 attatggcgt ttgcgctttt ggctttgttg tggtcctggt tcggcaaaat cgatattgtg
     4861 gcggcggctt cgggcaaaac ggtgtcgggc gggcgcagca aaaccatcca gccgctggaa
     4921 acggcggtgg ttaaggcggt acatgtgcgc gacgggcagc atgtgaaaca gggagaaacg
     4981 ctggcggaac tggaggctgt gggaacagac agcgatgtgg tgcagtcgga gcaggctttg
     5041 caggctgccc aattgtccaa actgcgttat gaagcggtat tggcggcatt ggaaagccgt
     5101 accgtgccgc atatcgatat ggcgcaagca cggtctttag gtctctccga tgccgatgtg
     5161 caatcggcgc aggtgttggc gcagcaccag tatcaggcat gggcggcgca ggatgcgcaa
     5221 ttgcagtcgg ctttgcgcgg ccatcaggcg gaattgcagt cggccaaggc gcaggagcag
     5281 aagctggttt cggtgggggc gatcgagcag cagaaaacag cagactaccg ccgtttgcgg
     5341 gccgacaatt ttatttcgga acatgcgttt ttggagcagc agagcaaatc ggtcagcaat
     5401 tggaacgatt tggaaagtac gcgcggtcag atgaggcaga ttcaggcggc cattgcacag
     5461 gcggagcaga atcgggtgct gaatacgcag aacctgaaac gcgatacgct ggatgcgctg
     5521 cgccaggcaa acgaacagat tgaccaatac cgcggccaaa cggataaggc aaagcagcgg
     5581 cagcagctga tgacaataca gtcgcctgcg gacggcacgg tgcaggaatt ggctacctat
     5641 acggtgggcg gtgtggtgca ggctgcccaa aaaatgatgg tgattgcgcc cgatgacgac
     5701 aaaatggacg tggaagtttt ggtattgaac aaagacatcg gttttgtgga acagggacag
     5761 gatgcggtgg tgaagattga gagctttccc tatacgcgct acggttatct gacgggcaag
     5821 gtgaaaagtg tcagccatga tgcggtaagc cacgaacagt tgggcttggt ttatacggcg
     5881 gtggtgtcgc tggacaaaca taccttgaat attgacggca aagcagtgaa tctgacggcg
     5941 ggcatgaatg tcacggcgga gattaaaacg ggtaaacggc gggtgctgga ttatctgtta
     6001 agcccgctgc aaaccaaatt ggacgaaagc tttagggagc gataggcgga tccgtactgg
     6061 gcatttgtta tccgccggtt cggacatgca gactgcctga aaccattgcc cggatgacat
     6121 tgctcaatct aatgataatg caagattacg gtatttccgt ttgcctgaca ctgacgccct
     6181 atttgcaaca tgaactattt tcggctatga aatcctattt ttccaaatat atcctacccg
     6241 tttcactttt taccttgcca ctatcccttt ccccatccgt ttcggctttt acgctgcctg
     6301 aagcatggcg ggcggcgcag caacattcgg ctgattttca agcgtcccat taccagcgtg
     6361 atgcagtgcg cgcacggcaa caacaagcca aggccgcatt ccttccccat gtatccgcca
     6421 atgccagcta ccagcgccag ccgccatcga tttcttccac ccgcgaaaca cagggatgga
     6481 gcgtgcaggt gggacaaacc ttatttgacg ctgccaaatt tgcacaatac cgccaaagca
     6541 ggttcgatac gcaggctgca gaacagcgtt tcgatgcggc acgcgaagaa ttgctgttga
     6601 aagttgccga aagttatttc aacgttttac tcagccgaga caccgttgcc gcccatgcgg
     6661 cggaaaaaga ggcttatgcc cagcaggtaa ggcaggcgca ggctttattc aataaaggtg
     6721 ctgccaccgc gctggatatt cacgaagcca aagccggtta cgacaatgcc ctggcccaag
     6781 aaatcgccgt attggctgag aaacaaacct atgaaaacca gttgaacgac tacaccgacc
     6841 tggatagcaa acaaatcgag gccatagata ccgccaacct gttggcacgc tatctgccca
     6901 agctggaacg ttacagtctg gatgaatggc agcgcattgc cttatccaac aatcatgaat
     6961 accggatgca gcagcttgcc ctgcaaagca gcggacaggc gcttcgggca gcacagaaca
     7021 gccgctatcc caccgtttct gcccatgtcg gctatcagaa taacctctac acttcatctg
     7081 cgcagaataa tgactaccac tatcggggca aagggatgag cgtcggcgta cagttgaatt
     7141 tgccgcttta taccggcgga gaattgtcgg gcaaaatcca tgaagccgaa gcgcaatacg
     7201 gggccgccga agcacagctg accgcaaccg agcggcacat caaactcgcc gtacgccagg
     7261 cttataccga aagcggtgcg gcgcgttacc aaatcatggc gcaagaacgg gttttggaaa
     7321 gcagccgttt gaaactgaaa tcgaccgaaa ccggccaaca atacggcatc cgcaaccggc
     7381 tggaagtaat acgggcgcgg caggaagtcg cccaagcaga acagaaactg gctcaagcac
     7441 ggtataaatt catgctggct tatttgcgct tggtgaaaga gagcgggtta gggttggaaa
     7501 cggtatttgc ggaataaagc aggctgaaac ggttatgaaa ttcccaaagc agcctgcacc
     7561 ccgtttcgaa agtgcaggct gctttgggat tgatccgata ttttcacatt ctcattgcct
     7621 ttaaataaag cacagttaaa aacacattta aattaagtgc gaattaattc aaatgtatat
     7681 tcaattaaaa tcaaaatagg acagtagtgc atcgttaaat cgggcgtaat cagacaatac
     7741 ggttcgcaga taccgcttaa tattcgccca aaccttctca atcgggttga gctcaggtga
     7801 ataaggtgca agaggcaata ccttatgtcc caatttttcc gccatttccc gtaagacacc
     7861 catacggtga aatcgtgcat tatctaaaat aatcaccgat ttttgagtca atgcgggcag
     7921 taggcattgc tgaaaccacg cttcaaaaaa gactccggtc atcgtatttt gataaaccat
     7981 cggagcaatc agccggttgc cgacttgtgc ggacaccaga gataagcgtc ggtatctttt
     8041 tccacttatc tgcgctttca ctatttgccc tttcaggctg cgggcatagg gacggaacag
     8101 gtggcggtca aatcctgttt catccaaata aacgcgttgg tagtcagaaa attcggccgg
     8161 ctgtgtcaaa taatgcgtta ctttggccgg gtcttgttct ttgtaagtgg tggtcttttt
     8221 ttgcgcgtta tccccatctg tttgagtgca tagcaaacgg tggctgccgt acaatcaaaa
     8281 tgtttggcga tttcatgcag ataggcatcc tggtgttgc
//
LOCUS       DAWHRA010000089         7911 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWHRA010000089 DAWHRA010000000
VERSION     DAWHRA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.089"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(89..1345)
                     /locus_tag="WIB30_001481"
     CDS             complement(89..1345)
                     /locus_tag="WIB30_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ3797534.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1382..2239)
                     /locus_tag="WIB30_001482"
     CDS             complement(1382..2239)
                     /locus_tag="WIB30_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ3797535.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(2300..3115)
                     /locus_tag="WIB30_001483"
     CDS             complement(2300..3115)
                     /locus_tag="WIB30_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ3797536.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            3179..3349
                     /locus_tag="WIB30_001484"
     CDS             3179..3349
                     /locus_tag="WIB30_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797537.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            3359..4144
                     /locus_tag="WIB30_001485"
     CDS             3359..4144
                     /locus_tag="WIB30_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ3797538.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            4414..4933
                     /locus_tag="WIB30_001486"
                     /pseudo
     CDS             4414..4933
                     /locus_tag="WIB30_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-denpendent receptor"
     gene            4938..6139
                     /locus_tag="WIB30_001487"
                     /pseudo
     CDS             4938..6139
                     /locus_tag="WIB30_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685727.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            6263..7813
                     /locus_tag="WIB30_001488"
     CDS             6263..7813
                     /locus_tag="WIB30_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ3797539.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         1896 a         1941 c         2204 g         1870 t
ORIGIN      
        1 gtactgattt ttgttaatcc actataattg atgaaatgcc gtctgaacgc tttgaaaagg
       61 gttcagacgg cattttgttc aaaccggttt atttgcgttg caatacggcg gcgaagaagc
      121 cgtcggtttg gtgtcgggcg gagttgaggc gcaggtattt gccggtatcc aaatcgattt
      181 tcaaaccggc aagcagttcg gcgcagttga cgggttcaaa ttcgggatgt tcggacagga
      241 aacgttcgac ttgcagctcg ttttcttcgg gcaggatgct gcaagtggcg taaaccaagc
      301 ggccttgcgg ttttaccagt ttggaggcag catcgaggat gctgtgttgc tgttccaaaa
      361 ggttggccac ggtctcggca gattggcggt atttgaggtc gggattgcgg cgtaaagtac
      421 ccaaaccgga gcagggcgcg tccaccaaca cacggtcggc tttgcctgcc agtcgggcga
      481 tacggctgtc gtgttcgctg ctgatgcgtt cggggtggat attggtcagt ccggcgcggg
      541 tcatacgcgg tttgaggttg gcaaggcgtt tttcggcgat gtcgaaggcg tagattctgc
      601 ctttattggc catttgcgca ccgacagcca aggttttgcc gccggcaccg gcacagaaat
      661 cgacaatgat ttcgccgcgt tttgcgccca ccaataaagc aagcagctgg ctgccttcgt
      721 cttggacttc cagtgtgccg tccaaaaaca gctcgtgttt gttaagcgcg attttgtttt
      781 tcaggcggat gccccaaggc gaataaggcg ttgcctctgc atcggcactt tcggcttgca
      841 acaatggcag cactttatcg cgtttgcctt tcaaggtatt gacgcgtata tcgagcggcg
      901 cagcttggtt gatgctgcgg ccgaaggcga ggatttcttc ttcgcgccaa tgctgctgca
      961 gctgttctac cagccattgc ggcaattctg cggcggtatt caggccgtct gaaaactcgg
     1021 ttttacgggc tttcaaattg ccgagaaact ctatttcttc ttcatcaagc aggtctttga
     1081 tttggctgat gttggtgctt ctgccgagaa ccagtgcggc gagtgcggct ttgcgcggct
     1141 gcgcgtgcgg acggcgcagg gcggtactga ttttttgata gtggcgcagc gcggcaaagg
     1201 cggtttcggc gatttcgtgg cggtcttggc ggccgagctt tttatgttcg cggaaatagg
     1261 cggagaggac ggcatcggca ggctgtttga aagtcagcat ttcagccaaa actttggcgg
     1321 tatggtcgag ttgtgcggcg ttcataatgt aatgggtttc tcttaaagtt ggtaaatggt
     1381 ttcagacggc cttcataatg cgttcgattt cgtgccaaaa gccgtccggc cgacattcca
     1441 aaacggcaat caagccttgg tcgatacggc ggatttcggt tgcgttcaat gtttcggttt
     1501 cggcgacacg cgcaggcgcg aggtaggcca tacggtcgag cagatggtag cggacttctt
     1561 ggcgtttaaa cccgtattcc gcccaatctt gaatatagat gccgcgccaa ccgtatggat
     1621 taagcggtgg ctcaaaagca tggaaaccgt gtggaaaaaa tccgatgccg cgcacgatgg
     1681 aaacgggttt tacgtcaagc ggcagaccgt gttgccgcaa ggttcggatg ccgtctgaag
     1741 tgtgcggcag accgagttgg gcggtcagtt tggcggcttt tcccaacagc gtgtctttgg
     1801 ggtcgagtcc gcgcatgtct tcgagcctgc ccgtgccgcc gccgtaatat ttgcaggtca
     1861 gctcgatatg gtagggcttg ccgttaagcc ttaccacaaa atccgccgcg ccttgcgtat
     1921 tgccgtccga accggaaacc gtgaggttgt gcgcgagcag ttcggcgtgt ggcgcgttgg
     1981 caaaccaaaa tgccagcagt tcttcggcgt aaatgccgag gcggtgtccg aacggggcgc
     2041 gtcgggtgag gtgttccgtc agcggcgcgg gatcggcatc caatgccaaa agatagcgga
     2101 aaccgcgttc ccccaatagt tcgcgcacgc ccaattcgca accgctttgc cacaaaggcg
     2161 gcgcagtcag cagcgaggcg aggtcgcgga cgggttggct ggtgagcttc caccataatg
     2221 cgtctagggc gtaattcatg cttatccgaa tatcagtcat gccgtctgaa aagctggaaa
     2281 tgttttcaga cggccttaat cagtcttttt tatggatacg atggctgacg gtattgaaaa
     2341 aaccgcgcag gatatcgatg tcttcggttt gcgtgttggc gcgtccgaac aggctctgca
     2401 tacggcgcat cagacgctcg ccgttgcggc ggttgaaaaa gccgatgtcg ttcatcacgc
     2461 tttccatgtg ggcgaccatg cctttgattt gctcgtgggt cgcagcgtgg tcttcttgtt
     2521 gaagatgggt catgggcgaa ccggtttggc tgaagatttc gtagcacacg acctgcacgg
     2581 cttgggcgag gttgagcgag aaatagtcgg gattgccgtt gatggtcatc agtcggttgc
     2641 aggcttggac ttcttcgatg ctcaagccga aagtctcgtt gccgaaaacc agtgccactt
     2701 tctcgcctcg gtttgcggtc tgcagtaatt cggataccaa atcgcgcggg gtttgcagcg
     2761 gcgcagtaat ttcgcggcgg cggctggtca gggcgcaggc gatggtggtg tcggcaaggg
     2821 cttcgtccaa agaagcggca atggtggcat tttccaaaac gtctgccgcg ccggaagcga
     2881 ggatgaagct ttcttccggt aatttaaacg attgaggatg ctccgggtca aacacgggcg
     2941 ggttttccgt catcggcgtt gccatcagat ttggggcgac gatggtcagt ttgtgcagac
     3001 ccattgtttt catcgcgcgc gcggcagagc cgatgttggc gggatggctg gtgcgcgtga
     3061 ggatgatgcg gatgttgtcc agataagcgg gcagggcggg tttgagagta gtcatgttcg
     3121 gttttcggtc ggaatggaat agggtataat gcgccgtctt tttcgttcgg acggtcaaat
     3181 gccgtctgaa atgttcttta acaacgtcgg aaatttaacg ttgcgcctga agcagacacg
     3241 cgtcgggcgg tattttaccc gatttggcag gatttacccc aaccaaataa actccgattt
     3301 tgccgctccg aaggactgcg cgtccgaacc ggcggtatgc cacccttaag gaaatctgat
     3361 gaatccgttt ttgaatacag cctttaaagc cgcccgccgt gccggtcaga tgatgatacg
     3421 cgccgcgggc aatctcgatg ccgtcaaaac cgacagcaaa gcattcaacg attttgtttc
     3481 cgatgttgac cgcaattccg aaatcatcct ggttgaggct ttgaaagaag cctatccgca
     3541 ccacaaaatc acttgcgaag aaagcggctc ccacggtaaa gccgcagccg aatacgaatg
     3601 gattatcgat ccgctcgacg gcacgaccaa cttccttcac ggccatcccc aatacgccat
     3661 ctctatggca ctcctgcaca aaggcgtgtt gcaagaagct ttggtgtacg cccccgaacg
     3721 caacgacgtt tacatggctt cgcgcggcaa aggcgcgttg ctcaatgacc gccgcatccg
     3781 cgtttccaac cgcatcgaat tgaaccgctg cctgatcggt accggctttc ctgttgtcga
     3841 tcaaagcatg atggacaagt atctggcgat tttgaaagat ttcttggcaa aaaccgccgg
     3901 cggccgtcgt gaaggcgcgg cttctttgga tttgtgtgct gtggcgactg gtcgttttga
     3961 cggctttttc gagttcaacc tcaaaccttg ggatattgcc gcaggcgcat tgattgttca
     4021 ggaagcgggc ggtatcgtaa ccgatatgac gggtgaagac ggctggctgg aaagcggcga
     4081 tattgttgcc gccaatccca aagtgctggc gcaaatgtta aaaatcattt ccgcacacgt
     4141 ttaaatttca gcccgctccc gggaatgcgg gaaaacgggc gggtatgccg tctgaagtat
     4201 cgcgccgata tttcagacgg catttttact ggcagcgggg gcaggaaggt ggaaacggct
     4261 aaatgcccga aactgccgaa gatggaaacc catatctgat taaacggaga accctgtacg
     4321 atttgtttat agtggattaa caaaaaccag tacggtgttg cctcgcctta gctcaaagag
     4381 aacgattctc taaggtgctg aagcaccaag tgaatgggca aagccaacgc ctttaccgca
     4441 ccgattaccg tcgtcaatta cgacgaacaa gccctcaata acaccgaagc gcgtactttg
     4501 gtggatgccg tagcgaaaaa agacgcttcc gtttggcagt tcggcggcga aagcaacacg
     4561 ctgaccggac tgtatttcag aggttatcag cttgatgcgc gccaataccg gtacggcccg
     4621 cctgaccgac caatacttcc gcactttaag catgaacctg tctgcgcgag gcgaatttga
     4681 aaccggcccg gtaacgcata actggagtac ggctttcgac cgcgttatcc gccagcggaa
     4741 aaccattcgc ggaacggcag ccggagctag caaggtagaa gtgaaggcaa acggaaatat
     4801 tgagcatcaa ttggcatcgt ttacagccga ctatcccaat tcttgggaga actctgccaa
     4861 cttggatgcc aatatcaaag tcaacagcct ggctttgtcc gacaccttgg gcttcgtgga
     4921 taacaaatac cgttggcgcg cccgatggta aaaccaacct gctgccttct tggaactggc
     4981 aaaataccgc cggccaaacc aatatgctta cgtttgaatg ggatgcgttt gacaatgccc
     5041 aaattaccgg cggtatcggc tacaacaagg cgcgttatta cggcacgctg atttccccga
     5101 ccgtttgcgg tacaagcggt gcgagcgacc aaaccgctac ctgctcaagt gccaagcaat
     5161 accacaccgg cacggcgagc ctgaccgacc aatatttccg cacattgagt atgaacctga
     5221 ccgcacgcgg cgaatttgaa accggtccgg taacacataa ctggagcacc gcgttcgacc
     5281 gtgttatccg ccagcggaaa accattcaga gcggtgatgc caaacgtttc agcccgatgc
     5341 tgatggcggc gtgggtgccg caacctgatt tggttgtgta cggcaactat atggaagact
     5401 tggagcctgc cgatatcaaa accgatgatt ccggcgaaac gacaatggcg aaaccgcgtg
     5461 tcagccgcca gtttgaaatc ggcgtgcgta aaaactgggg cgattttgta accactttaa
     5521 acgcattcca aatcaaacgc ccggctactg gcgcggtcat acaactacca agacaacaaa
     5581 aggggtaaca acaacgctga cctatggcaa caattccgat ttcgcccgct ataaggcgca
     5641 aggcggcgta gccggcgacg agcaaggtat ggaacgcaat cgcggtatcg agttcaatgc
     5701 ctatgctaac ctgttaaaca aaaccctgcg tccgactttg ggcctgatgt atctgcaatc
     5761 gaccgtgaaa gactacccaa attcacgcga tatgctggtt aacggcgtac aagtcgccaa
     5821 tccgcgcgta attgccaagg caggtttgga atgggataca ccgtttgcca aaggcttgac
     5881 tttaaacagc aatgtttcgt atttcggcaa gtcttaccaa gacacgcaaa aacaatacgc
     5941 cttcccatcc tataccttgg ttgatgtagg cgcgcgctac aaaaccaagc tcggcaaaaa
     6001 tacgttgacc gtcagcagct cggtggaaaa tctgttcaac aaaaactact ggcaggtaca
     6061 gcgcggccaa tacgaccgca gcttcgccgt cgtcggtatg ccgcgcactt actggctgaa
     6121 ggcggaattg gatttctgat ttccctatat ccagaaaatg ccgtccgaac aggtttcaga
     6181 cggcatttct tttaaagaga tatggcggga tacgcttaaa taatgctatt ctagcgtctt
     6241 tgaaacctgc ttgtttgaag tgatgaaaaa acctttgatt tcggttgcgg cagcattgct
     6301 cggcgttgct ttgggcacgc cttattattt gggtgtcaaa gccgaagaaa gcttgacgca
     6361 gcagcaaaaa atattgcagg aagcgggctt cttgaccgtc gaatcgcacc aatatgagcg
     6421 cggctggttt acctctacgg aaacgacggt catccgcttg aaaccggagt tgctgaataa
     6481 tgcccggaaa tacctgccgg acaacctgaa aacagtgttg gaacagccgg ttacgctggt
     6541 aaaccatatc acgcacggcc ctttcgccgg cggattcggc acgcaggcgc acattgaaac
     6601 cgagttcaaa tacgcgcctg aaacggaaaa agttttggaa cgcttttttg ggaaacaagc
     6661 tccggtttcc cttgccaata ccgtttattt taacggcagc ggtaaaatgg aagtcagtgt
     6721 tcccgccttc gattatgaag agctgtcggg catcaggctg cattgggaag gattgacggg
     6781 aaaaacggtt tatcaaaaag gtttcaaaag ctaccgcaac agctatgatg cgcccttgtt
     6841 caaaatcaag ctggcagaca aaggcgatgc cgcgtttgaa aaagcgcatt tcgattcgga
     6901 aacttcagac ggcatcaatc cgcttgcttt gggcagcagc aatctgacct tggaaaaatt
     6961 ctccctagaa tggaaagagg gtgtcgatta caacgtcaag ttaaacgaac tggtcaatct
     7021 tgttaccgat ttgcagattg gcgcgtttat caatcccaac ggcagcatcg caccttccaa
     7081 aatcgaagtc ggcaaactgg ctttttcaac caagaccggg gaatcaggcg cgtttatcga
     7141 cagtgaaggg cagttccgtt ttggcacgct ggtttacggc gatgaaaaat acggcccgct
     7201 ggacatccat atcgccgccg aacatctcga tgcttctgcc ttaaccgtat tgaaacgtaa
     7261 gtttgcacaa atttccgcca aaaaaatgac cgaggaacaa atccgcaatg atttgattgc
     7321 cgccgtcaaa ggcgaggctt ccggactgtt caccaacaat cccgtattgg acattaaaac
     7381 ttttcgtttc acacagccgt cgggaaaaat cgatgtgggt ggaaaaatca tgtttaaaga
     7441 catgaagaag gaagatttga accaattggg tttgatgctg aagaaaaccg aagccgacat
     7501 cagaatgagt attcctcaaa aaatgttgga agatttggcg gtaagtcagg ctggaaatat
     7561 tttcagcgtc aatgccgaag atgaggcgga aggcagggcg agtcttgacg acatcaacga
     7621 gaccttgcgc ctgatggtgg acagtacggt tcagagtatg gcaagggaaa aatatctgac
     7681 tttgaacggc gaccagattg atactgccat ttctctgaaa aacaatcagt tgaaattgaa
     7741 cggtaaaacg ttgcaaaacg aaccggagcc ggattttgat gaaggcggta tggtttcaga
     7801 gccgcagcag taatgggtca aatgccgtct gaaatgttca ggcggcattt tgttgtattg
     7861 gaaactgccg ttttcaacca ttcctttaga taagccgtgt cggattcgtt t
//
LOCUS       DAWHRA010000090         7844 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWHRA010000090 DAWHRA010000000
VERSION     DAWHRA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.090"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..215)
                     /locus_tag="WIB30_001489"
     CDS             complement(<1..215)
                     /locus_tag="WIB30_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ3797540.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRK"
     gene            complement(323..1240)
                     /locus_tag="WIB30_001490"
     CDS             complement(323..1240)
                     /locus_tag="WIB30_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3797541.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITTSEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            1413..2213
                     /locus_tag="WIB30_001491"
     CDS             1413..2213
                     /locus_tag="WIB30_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3797542.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSVMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            2261..3037
                     /gene="mlaE"
                     /locus_tag="WIB30_001492"
     CDS             2261..3037
                     /gene="mlaE"
                     /locus_tag="WIB30_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ3797543.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLSLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            3088..3582
                     /gene="mlaD"
                     /locus_tag="WIB30_001493"
     CDS             3088..3582
                     /gene="mlaD"
                     /locus_tag="WIB30_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ3797544.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGNSGKTYTVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            3619..4209
                     /locus_tag="WIB30_001494"
     CDS             3619..4209
                     /locus_tag="WIB30_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ3797545.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            4266..4544
                     /locus_tag="WIB30_001495"
     CDS             4266..4544
                     /locus_tag="WIB30_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ3797546.1"
                     /translation="MHTELNNGTLHISGDITVKTLTGEAFERFQQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            4541..5368
                     /locus_tag="WIB30_001496"
     CDS             4541..5368
                     /locus_tag="WIB30_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ3797547.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            5493..5855
                     /locus_tag="WIB30_001497"
     CDS             5493..5855
                     /locus_tag="WIB30_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ3797548.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            5852..6238
                     /locus_tag="WIB30_001498"
     CDS             5852..6238
                     /locus_tag="WIB30_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ3797549.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            6235..6723
                     /locus_tag="WIB30_001499"
     CDS             6235..6723
                     /locus_tag="WIB30_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ3797550.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(6805..6972)
                     /locus_tag="WIB30_001500"
     CDS             complement(6805..6972)
                     /locus_tag="WIB30_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ3797551.1"
                     /translation="MEDDVWPGRRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(6939..7313)
                     /locus_tag="WIB30_001501"
     CDS             complement(6939..7313)
                     /locus_tag="WIB30_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ3797552.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIVGRRVRGFLARRVSPHIGR
                     GVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFDRSKN
                     ALRATRKSVRLRWRTMSGRGAG"
     gene            7323..7426
                     /locus_tag="WIB30_001502"
                     /pseudo
     CDS             7323..7426
                     /locus_tag="WIB30_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7551..7766
                     /gene="rpmE"
                     /locus_tag="WIB30_001503"
     CDS             7551..7766
                     /gene="rpmE"
                     /locus_tag="WIB30_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ3797553.1"
                     /translation="MKQGTHPNYHEVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         1821 a         2274 c         1990 g         1759 t
ORIGIN      
        1 attttgcgca aatacgcgcc gcgttcgacc gcaggcaggc gttcccaagc cggttgcgcc
       61 gcgcgtgccg ccgccacagc tcggtcaacg tctgcttttc cgcctttggg ttcgcgggcg
      121 atggtttctt cggtggaagg gttcaatacg tcgcgccatt cgccgttgaa gtcgttttca
      181 aagcgtccgt tgatgtacat ggccaattgt ttcatttcgg gttctccagt tttgtagtca
      241 gatgtagttt tagtttattc ccaaataaat taccgtacaa gtttctttac acgcggattt
      301 cgggtttgaa atcaaatgcg gcttaccggt tttctttttt ggtttccgca ggcgtgctgc
      361 cgaaatattg cctgaatgcc tgagtaaagc tggaaacgtg gcggtagccg cataaatatg
      421 cggtttcgcc tatgcttttc cccccgtttt gcaacaggta gagcgcgtgc tgcatttgtt
      481 tgtggtgcag ccactcgctt gtggtaatgc cgaaatggtc gcgcatacgg cgttgcagcg
      541 tcctttcgct gatgttcagc gcgtctgtca gccggttgac ttggtgtgcg ccgtcggcaa
      601 acgcggcatt tagggtgcgg ctgaagtctt cagacggcat agcgtctgct tccgccgttt
      661 gccccgccgc cggcccgatg ccgtctgaaa ccgtgtccca caagtccgac agcagccgca
      721 acacgtccgc ctcgcggcgc aatgtttcgc ccaaatgccc ctttgggacg gctttcaggc
      781 aggatgccgc caagccgcgc aggttcgggg gcaaatccca tatcctgacc ggttcgcggt
      841 aaagcagggg tgcgaaacgc gcgtattccg gacgcagcag ccattgttcc atacctttaa
      901 tggtcatttt gaccgttttg ccgcctcggt aaagatagcg gctgaacagg acttcttccc
      961 cgacagcaat taggacaatc ttgccgccgt ccgcatcgat ttggaagcgg cagcggttga
     1021 tgccgaagtc caaactgcct tccagcaaga gcacgaacga cacataaggt tcggcgaggc
     1081 ggctgctgca aaaatcacac cgtgccgtta ctgtgccgcc gtggatggaa atgccgttgg
     1141 acaaagtatc gaaacggtag ctgccctcca catagctgcc gccgccgctt ttgaccagat
     1201 tgacgaaccg gctgctgttc aaatgttctg ctttttccat agttttctga taaaggttat
     1261 catttgaaag ataacatttt tcgccacagc aaacaatccg ccgccgacaa cggcattttc
     1321 acaacattta ccgtccgtat gccgtctgaa aattgcccgt cttcccgata agccttaaaa
     1381 tacacgccgt caaacctacc gtccgtccgt acatgagccc atcccccttt atcgaaatga
     1441 aagacgtcgc ttttgcgtat ggcgaccgcc cgattctgaa cgacatcaat ttcagcattc
     1501 cgcaaggcaa ttttgccgcc gtgatgggcg gttcgggcag cggcaaaacc acgctgatgc
     1561 ggctgattac gggtcagatc cgtccgcagt ccgggcaggt tttgattgaa ggacgggatt
     1621 tggcgggctt ttcggctgac gaactctacg aacaccgccg ccgcatgggc gtattattcc
     1681 aacacggcgc gctgtttacc gatttgtcgg tattcgacaa tatcgctttt ccgatgcgcg
     1741 aactgacacg cctgccggaa gcggtgattc gagatttggt tttgttgaaa ctgaacgcgg
     1801 tcggtttgcg cggcgtggaa aacctgatgc cgtccgagtt gtccggcggg atgtcgcgcc
     1861 gcgtcgcgct tgcccgcacg attgcgctcg accctgaaat tatgttgtac gacgaaccgt
     1921 ttaccggcct cgatccgatt tccttgggcg tgattgccca cttgatcagc cgcgtcaata
     1981 aggctttgcg ttcgaccagc gttatggtaa cgcacgacat tgaaaaatct ttggaaatcg
     2041 tcgatcaggt gatttttttg gcgcacggcg aaattatgtt ctccggctcg ccgcaggaaa
     2101 tgcgcgaact ggattcgcct tgggtgcgcc agtttgtcgg cgggctggca gacggccccg
     2161 tagcataccg ctatccggcg caaacgtcgt tgcagcagga tttgctcggg taaatgagca
     2221 ggtgccgtct gaaaccgttg aactttacat atagaaattt atgaatttta tccgttccgt
     2281 cggggcgaaa accctcggcc ttattcaatc cctcggcagt attacgctgt ttctgctgaa
     2341 cattctggcg aagtccggta cggctttcgt ccgtccgcgc ctgagcgtgc gccaagtgta
     2401 ttttgccggc gtgctgtcgg tgctgattgt tgccgtttcg gggctgtttg tcggtatggt
     2461 cttgggtttg cagggctata cgcagttgtc gaaattcaaa tccgccgata ttttgggcta
     2521 tatggttgcg gcttcgctgt tgcgcgaact gggtccggtg ttggcggcga ttctgtttgc
     2581 cagcagcgct ggcggtgcga tgaccagcga aatcggtttg atgaaaacga ccgaacagct
     2641 cgaagcgatg aacgtgatgg cggtaaaccc tgtcgcccgc gtggttgcgc cgcgcttttg
     2701 ggcgggcgtg ttttccatgc cgcttttggc ttcgattttc aacgtggcgg gtattttcgg
     2761 cgcgtatttg gtcggtgtaa gctggctgag cttggacagc ggcattttct ggtcgcaaat
     2821 gcagaacaac atcacgatac attacgatgt aatcaacggt ctgatcaaat ccgccgcgtt
     2881 cggcgtggcg gtaacgctga ttgccgtgca tcagggcttc cactgcgtcc cgacctcgga
     2941 aggcattttg cgcgccagca cgcgcacggt ggtttcgtcc gccctgacga ttttggcggt
     3001 cgattttata ttgaccgcgt ggatgtttac agattgacag ggcgtctgaa gacgaaacgc
     3061 ggaacatcgg atattcaagg aactttaatg aaaaagaaca tattggaatt ttgggtcgga
     3121 ctgttcgtcc tgattggcgc ggcggcggtt gcctttctcg ctttccgcgt ggcgggcggc
     3181 gcggcgttcg gcaattccgg caagacttat accgtatatg ccgatttcgg cgacatcggc
     3241 ggtttgaagg tcaatgcccc cgtcaaatcc gcaggcgtat tggtcgggcg cgtcggcgct
     3301 atcggacttg acccgaaatc ctatcaggcg agggtgcgcc tcgatttgga cggcaagtat
     3361 cagttcagca gcgacgtttc cgcgcaaatc ctgacttcgg gacttttggg cgagcagtac
     3421 atcgggctgc agcagggcgg cgacacggaa aaccttgctg ccggcgacac catctccgta
     3481 accagttctg caatggttct ggaaaacctt atcggcaaat tcatgacgag ttttgccgaa
     3541 aaaaatgccg acggcggcaa tgcggaaaaa gccgccgaat aaacgtatgc catcatattc
     3601 aaactaggac acttaatcat gaaaaaatcc tccctcatca gcgcattggg catcggtatt
     3661 ttgagcatcg gcatggcatt tgccgcccct gccgacgcgg taaaccaaat ccgtcaaaac
     3721 gccactcaag tattgagcat cttaaaaagc ggtgatgcca acaccgcccg ccaaaaagcc
     3781 gaagcctatg cgattcccta tttcgatttc caacgtatga ccgcattggc ggtcggcaac
     3841 ccttggcgca ccgcgtccga cgcgcaaaaa caagcgttgg ccaaagaatt tcaaaccctg
     3901 ctgatccgca cctattccgg cacgatgctg aaattaaaaa acgccaacgt caacgtcaaa
     3961 gacaatccca tcgtcaataa aggcggcaaa gaaatcatcg tccgcgccga agtcggcgta
     4021 cctgggcaaa aacccgtcaa catggacttc accacctacc aaagcggcgg taaataccgt
     4081 acctacaacg tcgccatcga aggcgcgagc ctggttaccg tgtaccgcaa ccaattcggc
     4141 gaaattatca aagcgaaagg cgtggacgga ctgattgccg agttgaaggc taaaaacggc
     4201 agcaagtaat ccataagcca tgccgccaga ctgaagatgg catggatttt ttcctaccga
     4261 acactatgca tacagaactc aacaacggaa cactgcacat cagcggcgac atcaccgtca
     4321 aaaccctgac cggggaggct tttgagcgtt tccagcagca atgccgtctg aaagaaacca
     4381 ttgccgtcga tttcggcggt gtcaaacgtg ccgattccgc ctgcgtgtcg ctgctgctcg
     4441 aagtgctgcg cggctgcaaa ggcagcgtca ggctgaccgg cattcccgaa tccgtgcgcg
     4501 cgctgtccga actgtacgaa atcaaagact ggctgaaatc atgaaaaaaa ccgcctatgc
     4561 cttcctcctg ctgatcgggt tcgcttccgc ccctgcattt gccgaaaccc gccccgccga
     4621 cccttatgaa ggctacaacc gcgccgtttt caaattcaac gaccaagccg accgctacat
     4681 tttcgcccct gccgcgcgcg gctaccgcaa agttgcgccg aaacccgtcc gcgccggcgt
     4741 gtccaatttt tttaataacc tgtgcgacgt ggtcagcttc ggcagcaata tcttgcgctt
     4801 ggacatcaaa cgcgcaagcg aagaccttgt ccgcgtcggc atcaacacca ctttcggttt
     4861 gggcgggctt atcgacatcg ccggcgcggg cggcattccc gacaataaaa acaccttggg
     4921 cgacacgttt gcttcgtggg gatggaaaaa cagcaattat ttcgtgttgc ccgtcttagg
     4981 gccgtccacc gtccgcgacg cgctcggcac gggtattacc tccgtttatt cgcccaagaa
     5041 tatcgtcttc cgcacccctg tcggacgctg gggcacgact gccgtatccg ccgtcagtac
     5101 gcgcgaaggc ctgctcgatt tgaccgacag cctggacgaa gccgccatcg acaaatacag
     5161 ctacacgcgc gacctctata tgaaagtccg tgcgcggcag accggtgcaa cacctgccga
     5221 aggtacggaa gataacatcg acatcgacga attggtcgaa agtgccgaaa ccggcgcggc
     5281 ggaaactgcc gttcaagaag attccgtatc cgaaacacag gcagaagcag caggggaagc
     5341 cgaaacgcaa cctggaacac aaccttaaag ccatgccgtc tgaaaatccg tgttcggacg
     5401 gcattccctt ctgtcggaac ggacagtccg aaaccacaga ttgggctata atctcttttt
     5461 tgcgcatacc gcgcgacagc aaaggaaaaa ccatgtacga agtcaaccgc agcgtattcg
     5521 tcctcatccc tttagaacct ttttggaact ggctgcaaac cctgccgggc aaccacctcg
     5581 acggactgac tcttgaagac atccaagccg acgccaattc ctaccttgtc cgcccgtgcg
     5641 aaaccgccga cgaagtatgg gacgaaatcg aagcccgttt tgaagacatt ttcgccgccg
     5701 aacttgccga ctggtgcgaa gacgaacgcg agtggcctgc gctcgatgcc gacattttca
     5761 acgaatggtt cgacatccag ctttctaccg tcatcaccga cctcgaacac gaaccgcttg
     5821 cccgcgaagc cttccaaccc atcaacctga actgatgaag ctgaccgtcc gcaactacca
     5881 tctcgacggc tacggccatg tcaacaatgc gcgctacctc gaatttttcg aagaagcgcg
     5941 ctgggcgttt tttgaagaac gcggactgct gcacgagttg gcaggcctca tactgattgt
     6001 cgcccgcatc gacatccgat acagccgccc cgccgtcgaa ggcgacgtat tgcagttttc
     6061 atgccgcctg aaaacccccg gcacgcgccg tattgtgctg acccagacga ttacactgcc
     6121 aaacggcaaa accgccgcag aagccgacat caccctgatg cccgtccacg ccgccacaca
     6181 acgcaccgtc agcctgcccg ccacccttgc ccgcgcattg gaagccttgt ccgaatgaaa
     6241 aaaatactca ccgccgccgt cgtcgcactg atcggcatcc tccttgccat cgtcctcatc
     6301 cccgacagca aaaccgcgcc cgccttctcc ctgcccgacc tgcacggaaa aaccgtttcc
     6361 aacgccgacc tgcaaggcaa agtaaccctg attaattttt ggtttccctc ctgtccgggt
     6421 tgtgtgagcg aaatgcccaa aatcattaaa acggcaaatg actataaaaa caaaaacttc
     6481 caagtccttg ccgtcgccca gcccatcgat ccgatagaaa gcgtccgcca atatgtcaaa
     6541 gactacggtt tgccgtttac cgtcatgtat gatgcggaca aagctgtcgg acaggcgttc
     6601 ggcacacagg tttatccgac ttccgtcctt atcggcaaaa aaggcgaaat cctcaaaacc
     6661 tacgtcggcg aacccgattt cggcaaactc taccaagaaa tcgataccgc gctggcacaa
     6721 tagcgatgcc gtctgaaacg caaaaaaaca gaaacgatgt tcctgttttt tcagatcatc
     6781 cgccccgaca cgttcagacg gcattcaacc ttccggcaga ttctttttca ccactgccgg
     6841 attgcctgcc gccaaagagt agggcggaat gtcttttgta accaccgcgc ctgcgcccac
     6901 gaccgaaccg cgtccgacgg ttacgcccgc cattacaatc accctgcgcc ccggccagac
     6961 atcgtcctcc aacgtaatcg gacggatttc cgtgtagccc tcaaagcgtt ttttgaacgg
     7021 tcaaacttgt ggttatttga ataaaacaga cattccggct ccatcatcac atttttgccg
     7081 accaccggcc cacggcagat ttcacagttt gccccgatgc ccgagccgtc gcccaaaacc
     7141 gtatccggaa acacatacgc cccgcgttcg atattgaccc cgcgtccgat atgcggagaa
     7201 acccgccgcg ccaaaaaacc gcgtacccgt ctgccgacga tgccgcgcat atgcgacggc
     7261 ggcaacaccg aaccggcaaa acgcagcagc aggaaaaaga attttgatcc catcaagcct
     7321 ccctcaaaag aactttttat agtggattaa caaaaatcag gacaaggcga cgaagccgca
     7381 gacagtacag atagtacggc aaggcgaggc aacgctgtac tggtttttgt taatccacta
     7441 tacattttat cgaatatcct cttgacgaaa caacagaaaa tagcgagaat gccgagcttg
     7501 tctgaagcat aggtacatcc cctatgcccg cttttttgaa aggaaatcaa atgaaacaag
     7561 gcactcaccc gaattatcac gaagtcaacg ttacctgctc ctgcggcaac aaatttgtaa
     7621 ccaaatccgc gatggaaaaa gaaaacttca atatcgaggt ttgctccctg tgccacccgt
     7681 tctataccgg cacccaaaaa atcgtcgata ccaccggccg cgtggacaaa ttcaacaaca
     7741 aattcggcaa cctgttcaaa cgctgattgc cgctttgcaa gaattaagcc ctgccatcat
     7801 ttcgatggca gggcttttta atatttggca cactgacttc ttat
//
LOCUS       DAWHRA010000091         7837 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWHRA010000091 DAWHRA010000000
VERSION     DAWHRA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.091"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            58..1227
                     /gene="prpF"
                     /locus_tag="WIB30_001504"
     CDS             58..1227
                     /gene="prpF"
                     /locus_tag="WIB30_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ3797554.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1641..2840
                     /locus_tag="WIB30_001505"
     CDS             1641..2840
                     /locus_tag="WIB30_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ3797555.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            2948..3568
                     /locus_tag="WIB30_001506"
     CDS             2948..3568
                     /locus_tag="WIB30_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ3797556.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(3695..4138)
                     /locus_tag="WIB30_001507"
     CDS             complement(3695..4138)
                     /locus_tag="WIB30_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ3797557.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4312..4680
                     /locus_tag="WIB30_001508"
     CDS             4312..4680
                     /locus_tag="WIB30_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ3797558.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            4725..5879
                     /locus_tag="WIB30_001509"
     CDS             4725..5879
                     /locus_tag="WIB30_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ3797559.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(5936..6808)
                     /locus_tag="WIB30_001510"
     CDS             complement(5936..6808)
                     /locus_tag="WIB30_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ3797560.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            6893..7705
                     /locus_tag="WIB30_001511"
     CDS             6893..7705
                     /locus_tag="WIB30_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ3797561.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSSEAGKVMNTLL
                     VYGRDGVRTGLYHKMHLFGFSGLGERYAEADTISAGRAVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRTRAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1686 a         2157 c         2282 g         1712 t
ORIGIN      
        1 cctaatccac tataaaaatc gtgggcagag cccacgctac ataaggagaa tctagaaatg
       61 ccgcaaatta aaattcccgc cgtttactac cgtggcggta catcaaaagg cgtgtttttc
      121 aaacgttccg acctgcccga ggcggcgcgg gaagcgggaa gcgcacgcga caaaatcctc
      181 ttgcgcgtac tcggcagccc ggacccctac ggcaagcaga tagacggttt gggcaacgcc
      241 agctcgtcca ccagcaaggc ggtgattttg gacaagtccg aacgcgccga tcacgatgtc
      301 gattaccttt tcgggcaagt ttccatcgac aaaccttttg tcgattggag tggcaactgc
      361 ggcaacctca ccgccgccgt gggcgcattt gccatcgagc aaggcttggt cgataaaggc
      421 aagattcctt cagacggcat ctgcacggtc aaaatctggc agaaaaacat cggcaaaacc
      481 attattgccc atgtaccgat gcaaaacggc gcagttttgg aaacaggcga ttttgagctc
      541 gacggcgtaa cgttcccggc agccgaagta caaatcgaat ttcttgatcc agccgacggt
      601 gaaggcagta tgttcccaac cggcaatttg gtcgatgaaa ttgatgtgcc gaatataggc
      661 cgtttgaaag ccacgctcat caacgcgggc attccgaccg ttttcctgaa tgccgccgac
      721 ttgggctaca cgggcaaaga gttgcaagac gacatcaaca acgatgccgc ggctttggaa
      781 aaattcgaga aaatccgcgc ttacggtgcg ctgaaaatgg gtctgatcag cgacgtatcc
      841 gaagctgccg ctcgcgcgca cacgccgaaa gtcgccttcg tcgcgcccgc cgccgattac
      901 accgcctcca gtggcaaaac cgtgaacgcc gccgacatcg atttgctggt acgcgccctg
      961 agcatgggca aattgcacca cgcgatgatg ggtaccgcct ctgttgccat tgcgaccgcc
     1021 gccgccgtgc ccggtacgct ggtcaacctt gccgcaggcg gcggaacgcg taaagaagtg
     1081 cgcttcgggc atccttccgg cacattgcgc gtcggtgcag ccgccgaatg tcaggacgga
     1141 caatggacgg ccaccaaagc ggttatgagc cgcagcgcac gcgtgatgat ggaaggttgg
     1201 gtcagggtgc cggaagattg tttttaaatt gacgtagcat gggtttgccc gcgagccata
     1261 aacaggtcgt ctgaaaaaca agtaaacatc aaatcactga ccattccttt cccttgccct
     1321 gtggcggaag gcggcaaatc acaaggaaga acacggaaac cccgataaaa gacagcttcc
     1381 cgtattaccg tcattcccgc gcaggcggga atctagacct gtcaatatgg aggattggta
     1441 ggggaaaaca ggtttcgtga gttctacatt ctggattccc gccacagcct gtcctcgcgt
     1501 aggcggggac ggaataacga tagaaaatgc ggcatacgct ttgcccaaag aggccgtctg
     1561 aaacaccttg cgcctgatgt ctgccttttt cagacgaccc cacaccaaaa aaacaaccac
     1621 aaactacaag gagaaacatc atgtccgacc aactcatcct cgttctgaac tgcggcagtt
     1681 catcgctcaa aggcgccgtt atcgaccgaa aaagcggcag cgtcgtccta agctgcctcg
     1741 gcgaacgcct gaccacgccc gaagccgtca ttacgttcaa caaagacggc aacaaacgcc
     1801 aagttcccct gagcggccga aattgccacg ccggcgcggt gggtatgctt ttgaacgaac
     1861 tggaaaaaca cggtctgcac gaccgcatca aagccatcgg ccaccgcatc gcccacggcg
     1921 gcgaaaaata cagcgagtct gttttgatcg accaggccgt aatggacgaa ctcaatgcct
     1981 gcattccgct tgcgccgctg cacaaccccg ccaacatcag cggcatcctt gccgcacagg
     2041 aacatttccc cggtctgccc aatgtcggcg tgatggatac ttcgttccac caaaccatgc
     2101 cggagcgtgc ctacacttat gccgtgccgc gcgagttgcg taaaaaatac gctttccgcc
     2161 gctacggttt ccacggcacc agtatgcgtt acgttgcccc tgaagccgca cgcatcttgg
     2221 gcaaacctct ggaagacatc cgcatgatta ttgcccactt aggcaacggc gcatccatta
     2281 ccgccatcaa aaacggcaaa tccgtcgata ccagtatggg tttcacgccg atcgaaggtt
     2341 tggtaatggg tacacgttgc ggcgacatcg atccgggcgt atacagctat ctgacttccc
     2401 acgccgggat ggatgttgcc caagtggatg aaatgctgaa caaaaaatca ggtttgctcg
     2461 gtatttccga actttccaac gactgccgca ccctcgaaat cgccgccgac gaaggccacg
     2521 aaggcgcgcg cctcgccctc gaagtcatga cctaccgcct cgccaaatac atcgcttcga
     2581 tggctgtggc ctgcggcggc gttgacgcac tcgtgttcac cggcggtatc ggcgaaaact
     2641 cgcgtaatat ccgtgccaaa accgtttcct atcttgattt cttgggtctg cacatcgaca
     2701 ccaaagccaa tatggaaaaa cgctacggca attcgggcat tatcagcccg accgattctt
     2761 ctccggctgt tttggttgtc ccgaccaatg aagaactgat gattgcctgc gacactgccg
     2821 aacttgccgg catcttgtag ccaaaaaagg gacgagtccg caaaaatgcc gtctgaaatt
     2881 ttcagacggc attttgtttg gattttgggc gtacaatacg gaaaacatga cgataaggac
     2941 acaaaccatg gcacagtttt tcgctattca tcccgacaat ccccaagaac gcctcatcaa
     3001 gcaggcggtt gaaatcgtca atcaaggcgg cgtggtggtt tatccgaccg attcgtgtta
     3061 cgccttgggt tgcaaactcg gcgataaggc ggcgatggaa cgcatactct ccatccgcaa
     3121 aatcgatttg aaacaccacc tgaccctgat gtgcgccgat ttgagcgagt tgggcacgta
     3181 cgccaaaatt gacaacgtac agtttcgtca gcttaaagcc gccacgcccg ggccttatac
     3241 ctttatcttg caggcgacga aggaagtgcc ggcgcgcacg ctgcacccga aacgcaaaac
     3301 catcgggctg cgtattcccg ataatgccat tgcacaagcc ctgctggggg aattgggcga
     3361 gccgctttta agctgcaccc tgatgctgcc cgaagacggc gaaccattga ccgatcctta
     3421 tgaaatccgc gagcgtttgg aacacgccgt cgatttggtg attgacggcg gctggtgcgg
     3481 aaccgagccg accaccgtcg tcgatatgac cgacggcacg gaactggtgc gccggggttg
     3541 tggcgatacg gcggtgttcg gtttgtaggg aaaccgatgc cgtctgaagc atcggctgtt
     3601 cagacggcat tgcgcgcctt gccggcggca gtccgaaatg ccggcgcgta tcgcgctcgg
     3661 tcggaatatc cgtttgaaac ggcattttga tgcattactg caccgcaatc ggaattctcg
     3721 gttcgtagag caggtcgtag gtcggcttgt tgagcaggtc ttggagcgtg aaaccgtcca
     3781 gatacgtgaa aaacgacttc attgcaccgc cgagtatgcc tgtcagacgg caggacggtg
     3841 taatcaggca ttcgttgttc gaacccatac attcgaccag ctgcatcggt tcgaggtggc
     3901 ggacgaccga gccgatgttg atgcggtcgg gcggtgtggc aagccgcaga ccgccgcctt
     3961 ttccgcgcac actgtggagg aagccgcctt tgaccagcgc ggtaacgacc ttcatcagat
     4021 ggcttttgga aatgccgtag gttacggcga tggtactgat gttgaccagc gcatcgtcgt
     4081 tgatggcagt gtagataagg acgcgcagcc cgtagtccgt atgttgtgtc aaatacatga
     4141 ttttctcggt atggattgtt attcttatcg gtacggttta aggttcacgg acaatacctt
     4201 aatggttgaa accctgtccg tcggggcggt agaatgcagc ctgtctgcgg cggtatgccg
     4261 tctgaaacat ccgcgctacc gtttgagaat ttgttattgt aactcaaaat catgaaaccg
     4321 ttgaaacgac atcccgccct tatcgggctt tcgcgtgacc accaccattc gctttccttg
     4381 tgcgtgcgtc tgttgcggac gccggaagaa aggcatcggg acgaactcga accgcatttt
     4441 tccgaattgg aaacccattt tcgcgaagaa gaaaccaagt ttgccccaat ttggcagaat
     4501 gtcgcccccg aattgaaaca acgtttcgag aaagaccacg cccgactgcg gcagatgatg
     4561 gcaagccccg aatacggtaa cgcggcgtgg aataccgctt ttgccacaac cctgcgcgac
     4621 cacgcgcgct ttgaagaacg cgagctgttt cccgccgccg aaccgttttt gccggcatga
     4681 ttccgttttg cggcaaatat attaatgata aacaaggaac acacatgaaa tttaccaagc
     4741 accccgtctg ggcaatggcg ttccgcccgt tttattcgct ggcggctctg tacggcgcat
     4801 tgtccgtatt gctgtggggt ttcggctaca cgggaacgca cgagctgtcc ggtttctatt
     4861 ggcacgcgca tgagatgatt tggggttatg ccggactggt cgtcgtcgcc ttcctgctga
     4921 ccgccgtcgc cacttggacg gggcagccgc ccacgcgggg cggcgttctg gtcggcttga
     4981 ctatcttttg gctggctgcg cggattgccg cctttatccc gggttggggt gcgtcggcaa
     5041 gcggcatact cggtacgctg tttttctggt acggcgcggt gtgcatggct ttgcccgtta
     5101 tccgttcaca gaatcaacgc aactatgttg ccgtgttcgc gctgttcgtc ttgggcggca
     5161 cgcatgcggc gttccacgtc cagctgcaca acggcaacct aggcggactc ttgagcggat
     5221 tacagtcggg cttggtgatg gtgtcgggtt ttatcggtct gattggtacg cggattattt
     5281 cgttttttac gtccaaacgg ttgaatgtgc cgcagattcc cagtccgaaa tgggtggcgc
     5341 aggcttcgct gtggctgccc atgctgaccg ccatgctgat ggcgcacggc gtgatgcctt
     5401 ggctgtcggc ggctttcgcg tttgcggcag gcgtgatctt taccgtgcag gtgtaccgct
     5461 ggtggtataa gcctgtgttg aaagagccga tgctgtggat tctgtttgcc ggctatctgt
     5521 ttaccggatt ggggctgatt gcggtcggcg cgtcttattt caaacccgct ttcctcaatc
     5581 tgggtgtgca tctgatcggg gtcggcggta tcggcgtgct gactttgggc atgatggcgc
     5641 gtaccgcgct cggtcatacg ggcaatccga tttatccgcc gcccaaagcc gttcccgttg
     5701 cgttttggct gatgatggcg gcaaccgccg tccgtatggt tgccgtattt tcttccggca
     5761 ctgcctacac gcacagcata cgcacctctt cggttttgtt tgcactcgcg cttttggtgt
     5821 atgcgtggaa gtatattcct tggctgattc gtccgcgttc ggacggcagg cccggttgag
     5881 acaaaccgcc gcagatttcg ggtctgggcg gtttgctttt cagacggcag ggcggtcagt
     5941 tgccgtccag ccagcggtcg cgtgtggttt tggcctcttc aaaatagcgg tacagggctt
     6001 cgcggtcgtc ggtggtcagg atgtttgcca aaacgtccaa ctgtttgccc aagccttgaa
     6061 ccagttgcag caggctgtct ttgttggcaa ggcagatgtc cgcccacacg gcgggatggc
     6121 cggaggcgat gcgggtgaag tcccgaaagc ccgtggcggc gaatttcaga tattcctgtc
     6181 cgtcggggtg gtcgagaatc tggtggacat aggcgaaggc ggtcaggtgg ggcatatggg
     6241 agacggcggc gaaaaccgcg tcgtggcgtt gcgcgtccat cgtataaatt tccgcaccga
     6301 ccgcgtgcca caggttttct atcaaggcaa tgccgtctga atgttcgccg ccgtgtggcg
     6361 tgatgatgag ttttctgtgg cggaacagcc cgaactgcgc ggcttgcgca ccgcttctgt
     6421 ccgaaccggc aattgggtgg gcggcgatgc agtggtgcag gcggtcgggc agacagcggc
     6481 ggaaggcttc gatgaccgaa gatttggtgc tgccgacatc ggaaatccaa gtgtgttccg
     6541 gcaaaacggg gcgcagcgcg gtcaaaatgg cgggaacggt ggcgacgggc gtggcaatca
     6601 gtaccaagtc cgcaccgccg atgctgtccg cgtcgatggc aacggaagcc cggtcaatca
     6661 cgccgcgttc caatgcacgt tcgaggttgt cgcggtcggt gtcgataccg gtaacggtgc
     6721 ggacgagtcc ctgccttttg aggtcgagaa cgaacgaacc gccgatcagc cctacaccga
     6781 tgagggcaat atggttcaaa atgggcattt gtgtaaacgg ttttcgcaaa gtaccgtcat
     6841 ggtagcctat cggcggaata tgccgcaagg tcggcaggaa aaaggagaag aaatggacaa
     6901 aatcagagtt gccgccgtgc agatggtgtc gggcgtgtcg ccggaaacca acgtcgctgc
     6961 catgaaacgc ctggtcgcac gggcggcgga gcagggggcg gattgggtgc tgctgcccga
     7021 atattgggtg ctgatgggcg caaacgatac cgacaaactc gcgcttgccg agcctttggg
     7081 cggcggacgc tttcagacgg cattgagcga aacggcgaaa gaatgcggcg tggtgctgtt
     7141 tggcggaact gtgccgctgc aaagcagtga ggcaggcaag gtgatgaata cactgttggt
     7201 gtacggacgg gacggcgtaa gaacgggtct gtaccacaaa atgcacctct tcggcttttc
     7261 cggtttgggc gaacgctatg ccgaagccga taccatcagc gcagggcggg cagtgccgca
     7321 cttgtcggca gagggcgtgc cggtggcggc aggtatttgt tacgatgtcc gctttcccga
     7381 atttttccgc cgccagctgc cgtttgacgt attgatgctt ccggcggcat ttacccatac
     7441 gacgggcaag gcgcactggg agctgctttt gcgtacgcgt gccgtcgaaa accaatgtta
     7501 tgttgtggcg gcggcacagg gcggattgca cgaaaacggc aggcgcacgt tcggacacag
     7561 tatgattgtc gatccgtggg gcgacgtgtt ggacgtattg cccgaaggcg aaggcatcgt
     7621 tacggcagac attgatgcca accgcctgaa cagcgtccgc aaccgcctgc ccgccctgaa
     7681 ataccgggtt ttggatgccg tctgaaggtt cagacggcat cggtgccggg gaatcagcgg
     7741 gctgtaggat gggcttcagc ctgccatttc catcaatccc acatttctac cgttttcatc
     7801 gaatccatcg aatccgcccc tttcgacgac ccagtcc
//
LOCUS       DAWHRA010000092         7809 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWHRA010000092 DAWHRA010000000
VERSION     DAWHRA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.092"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            227..1069
                     /locus_tag="WIB30_001512"
     CDS             227..1069
                     /locus_tag="WIB30_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ3797562.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVAV"
     gene            1099..1563
                     /gene="nrdR"
                     /locus_tag="WIB30_001513"
     CDS             1099..1563
                     /gene="nrdR"
                     /locus_tag="WIB30_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ3797563.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            1595..2704
                     /gene="ribD"
                     /locus_tag="WIB30_001514"
     CDS             1595..2704
                     /gene="ribD"
                     /locus_tag="WIB30_001514"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ3797564.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALVRAGVSRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLFSLL
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            2899..4317
                     /locus_tag="WIB30_001515"
     CDS             2899..4317
                     /locus_tag="WIB30_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ3797565.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSSQLVSKLAILLLLLTV
                     GLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIPI
                     ALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRA
                     IEENAPPARLSATAEFAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPPL
                     FCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFWL
                     FFAFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAAYLAGC
                     ILRHRKDLHKLFHYLKKQGFPL"
     gene            4314..5387
                     /locus_tag="WIB30_001516"
     CDS             4314..5387
                     /locus_tag="WIB30_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ3797566.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLRQHGHDIMLASSDGPLLK
                     EAQALGIRWQPVDFYRGGLFGYLKGMIAYARMLRREQPDIIDCQMARVVPACAIAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPADRIVYAY
                     NALPPPPEFPFWKTVKGCVMLGTLSRLDTVRAVHLMLDIFKKMVDRDMPVRLNVAGIG
                     EEMDNLKAQAERLGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILE
                     AGLYDTPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLREAMGKRL
                     HAHVAALCSDDEIYRTTMAAYTM"
     gene            5407..6572
                     /gene="pglH"
                     /locus_tag="WIB30_001517"
                     /pseudo
     CDS             5407..6572
                     /gene="pglH"
                     /locus_tag="WIB30_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
     gene            6565..7806
                     /locus_tag="WIB30_001518"
     CDS             6565..7806
                     /locus_tag="WIB30_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEZ3797567.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPPFTGKRKLAVVGAGGHGKVVADLA
                     AALGRYREIVFLDDRAQGSVNGFSVIGTTLLLENSLSPEQYDVAVAVGNNRIRRQIAE
                     KAAALGFALPVLVHPDATVSPSATVGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLNAFVHISPGAHLSGNTHIGEESWIGTGACSRQQIRIGSRATIGAGAVVVRDVSDG
                     MTVAGNPAKPLPRKNPKTGTA"
BASE COUNT         1693 a         2559 c         1988 g         1569 t
ORIGIN      
        1 cgcctgcgcg ggaatgacgg ctcaaaagtt acggaacgaa aaacaaccaa aaccggacaa
       61 gtcggattcc cgcctgcgcg ggaatgacgg aatcttaagt ttccgtcttt gttttctgtt
      121 ttctgttttc gagggaataa tggggaacaa gccgtatttc agacggcatt ttcagttcgg
      181 ggtataatcc gaatacttgc gaccatctga atcattggga caaaccatgt gtcaactgct
      241 gggcatgaac tgcaatacgc cgaccgatat tatgttttcc tttgaaggct tccgccgcag
      301 gggcggcatt accgaccacc atgccgacgg tttcggtatc ggctttttcg aaggcaaagg
      361 cgtgcgcctg ttccacgacg acaagccgag cgcaaattcc cccgtcgccg acctcgtgcg
      421 tgcctaccaa atcaaatcgg aaaacgtcat cgcacatatc cgcaaagcat cgcaaggaca
      481 aacctcgctg gcgaacaccc atccctttat gcgcgaaatg tggggcggct actggctgtt
      541 tgcccacaac ggacatttga ttgatttttt ccccgaacag ggcgaatttt tccaccccgt
      601 cggcacaacc gattccgaac gcgcgttctg ccacatcctc aaccgcctgc gcacccgctt
      661 tgccgcccgt cccgacgacg acacgctgtt tgacgcgatt gcggggctga cgcacgaaat
      721 ccgcaagttc gggctgttta actttatgct ttcagacggc attgccctgt ttgcccacgc
      781 cagcacgctg ctgcactaca tcgtccgcca agccccgttc ggcaaggcgc gcctgctcga
      841 cgacgacgtg atggtcgatt ttgccgaagt aaccacgccc tccgaccgcg tcgccgttat
      901 cgccaccctg ccactgaccc gcgacgaatc atggtcccaa cttgccgtgg acgaactggt
      961 catgttccgc gaaggcaaca tcgtccgaca cgaccgtccc gaaaaccccg tctatatgag
     1021 tgccgaagaa ggtctggaaa tcgcccgcgc cgccggcgtc gccgtctgaa cttcagacga
     1081 cataggagga cgaacccgat gaaatgcccg ttttgcgccc accccgacac ccgcgttgcc
     1141 gattcgcgtc tgatggaaga acgcaacgcc gtgcgccgcc gccgccactg ccccaactgc
     1201 ggcaaacgct tcggcacgct cgaaaccgcc gaactcaaaa tgcccgccgt catcggtccg
     1261 gacaaaaaac gctcgccctt taatgcacaa cgcctccgca acgacctgac cgctgccgcc
     1321 cgaaaatccg ccctgacacc cgaacagatc gacgaaaccg tccgcctgac ggaacacagg
     1381 ctctacactt cgggtcagcg cgacatcccc tctgccgcac ttgccgacat ggtgctaaaa
     1441 gaactgctcc gacaagatac ggaagccgcc gtccgtttcg ccgccctgca caaacgcttc
     1501 gacaatccgg cagactttgc ctcgtggctg gcgcaagccg tcaaaacagg cggcaaagcc
     1561 tgattccccc aacccatact gatacggtat ccctatgttt tcggacacag atatatccat
     1621 gatggaaaac gccctccgac ttgccgcttt ggggcgtttt tccacttcgc ccaatccgcg
     1681 cgtcggctgc gttatcgcac acggcagcca aattgtcggg caaggcttcc acgtcaaagc
     1741 gggcgaaccc catgccgaag tccacgccct gcgtcaggcg ggcgaaatgg cacaaggcgc
     1801 gaccgccttt gttaccctcg aaccgtgcag ccattacggg cgcacaccgc cctgtgccga
     1861 agcactggtg cgggcgggcg tgtcccgcgt cgttgccgcc atgcgcgacc ccaacccgct
     1921 ggttgcaggc aaagggcttg ccctgctcga agcagcaggc atcaagacgg aatgcggttt
     1981 actcgaacat caggcaaggg aactcaaccg aggcttcctg tcgcgcatcg aacgccgccg
     2041 cccctttgtc cgcctcaaat gcgccgtttc gctggacggc aaaaccgccc tttcagacgg
     2101 cagcagcttt tggattaccg gcgaagacgc gcgtgccgac gtacaggttt tgcgtgccga
     2161 aagctgcgcg gtgctgaccg gcatcggcac ggtgttggcg gacaatcccc ggctcaacgt
     2221 ccgcgctttt ccaactttgc gccaacccgc acgcatcgtt ttagacagcc gcctgcgcct
     2281 gcccccgaac agccatttgg ttaccgacgg acaatctccg acctacatcg ccacactcga
     2341 acgcaacgaa gacagactgc acccctatcg ggaacacgca cacgtccgca tcctgatgcc
     2401 gtctgaaacg gcagacagca aaatcgacct gcaccacctg atgcgcctcc ttgctgacga
     2461 aggtttcggc gaaatcatgg tcgaagcagg ctccgaactc acatccgcat ttttggcaga
     2521 aaatctggca gacgaaatcg tcctgtaccg ttcgcccaaa atcctcggca gcggcaaaga
     2581 cctgttttcc ctgctcgaaa accgcgccgc cctttccgca ccgcccttgt ggacacccgt
     2641 ttcaagcgaa atcctcggac acgacatcaa aaccgtgttc cgaaaaaacg gcaacgcctt
     2701 ttaaagggtt tgcgccgttt cactatataa taacgccgat aaaaaacggc cccgttcaga
     2761 ccgccgcccg cccgaaaaaa cgcaacccgg actgccgcac cccgccggca gccgcgacgg
     2821 tctgaaagcc gtcaaattcc gatcaagaaa ggcttcagac ggcacaggca gcatcccgcc
     2881 gccgcccgga catcaaaaat ggacacaaaa gaaatcctcg gctacgcggc aggctcgatc
     2941 ggcagcgcgg ttttagccgt catcatcctg ccgctgctgt cgtggtattt ccccgccgac
     3001 gacatcgggc gcatcgtgct gatgcagacg gcggcggggc tgacggtgtc ggtgttgtgc
     3061 ctcgggctgg atcaggcata cgtccgcgaa tactatgccg ccgccgacaa agacactttg
     3121 ttcaaaaccc tgttcctgcc gccgctgctg tctgccgccg cgatagccgc cctgctgctt
     3181 tcccgcccgt ccctgccgtc tgaaatcctg ttttcgctcg acgatgccgc cgccggcatc
     3241 gggctggtgc tgtttgaact gagcttcctg cccatccgct ttctcttact ggttttgcgt
     3301 atggaaggac gcgcccttgc cttttcgtcc tcgcaactcg tgtccaagct cgccatcctg
     3361 ctgctgctgc tgacggtcgg gctgctgcac tttccggcga acaccgccgt cctgaccgcc
     3421 gtttacgcgc tggcaaacct tgccgccgcc gcctttttgc tgtttcaaaa ccgatgccgt
     3481 ctgaaggccg tccggcgcgc accgttttca tccgccgtcc tgcatcgcgg cctgcgctac
     3541 ggcataccga tcgcactaag cagcatcgcc tattgggggc tggcatccgc cgaccgtttg
     3601 ttcctgaaaa aatatgccgg cctagaacag ctcggcgttt attcgatggg tatttcgttc
     3661 ggcggggcgg cattattgtt ccaaagcatc ttttcaacgg tctggacacc gtatattttc
     3721 cgcgcaatcg aagaaaacgc cccgcccgcc cgtctctcag caacggcaga attcgccgcc
     3781 gccctgcttg cctccgccct ctgcctgacc ggcattttct cgcccctcgc ctccctcctg
     3841 ctgccggaaa actacgccgc cgtccggttt atcgtcgtat cgtgtatgct gcctccgctg
     3901 ttttgcacgc tggtagaaat cagcggcatc ggtttgaacg tcgtccgaaa aacacgcccg
     3961 atcgcgctcg ccaccttggg cgcgctggcg gcaaacctgc tgctgctggg gcttgccgta
     4021 ccgtccggcg gcgcgcgcgg cgcggcggtt gcctgtgcca cctcattctg gttgtttttt
     4081 gccttcaaga ccgaaagctc ctgccgcctg tggcagccgc tcaaacgcct gccgctttat
     4141 acgcacacat tgctctgcct gacctcctcg gcggcctaca cctgcttcgg cacaccggca
     4201 aactaccccc tgtttgccgg cgtatgggcg gcatatctgg caggctgcat cctgcgccac
     4261 cggaaagatt tgcacaaact gtttcattat ttgaaaaaac aaggtttccc attatgaaaa
     4321 tcgttttgac cacatctatg gcaggcttgg gcggcacgga aaccgccacc gtccgcctcg
     4381 gcaggctact caggcagcac ggacacgaca tcatgcttgc ctcttcggac ggaccgttgc
     4441 tcaaagaggc gcaagccttg ggcatacgtt ggcaacccgt cgatttctat cgcggcggcc
     4501 ttttcggcta cctcaaaggt atgattgcct acgcccgaat gctgcggcgc gaacaacccg
     4561 acatcatcga ctgccaaatg gcgcgcgtcg tacccgcctg cgccattgcc gccaaaatcg
     4621 tttcgccgaa aaccaaaatc atctaccact cgcacggttt ggacgcggca acctacccca
     4681 aaatcgccaa actcttcgac aagctgggcg tgtacatcat cggtaactgc aaacacgaac
     4741 gcgaaaaact catccgccac ggtttccccg ccgaccggat tgtctacgcc tacaacgcac
     4801 tccccccccc cccggaattt cctttctgga aaaccgtcaa aggctgcgtc atgctcggca
     4861 cgctgtcgcg tctggacacc gtccgcgccg tgcatctgat gctggacatt ttcaaaaaaa
     4921 tggtcgaccg cgatatgccc gtacgcctga acgtggcggg cataggcgaa gaaatggaca
     4981 atttgaaagc gcaggcggaa cgtctgggca tagacggcaa agttaccttc ctcggcggcg
     5041 tgcgcgattt gaccgcctac ttcaaagacg tggatatttt ggtgaacacg ccccactgca
     5101 tcggcgacca cggcgcaggt gtgggcaaca acatcttaga agccggactt tacgacacac
     5161 ccgtcgttac ctacgatatg gcgggcattt ccgaaatggt cgtcaacggg gaaacgggtt
     5221 actgcatccc cttcggcgac gaagaagcct ttatcgccgc cgtagacacg ctcgtcagac
     5281 aaccgctgtt gcgcgaagca atgggcaaaa ggctgcacgc acacgtcgcc gcactctgtt
     5341 ccgacgacga aatctaccgt accaccatgg cggcctatac catgtaatgc cgtctgaaag
     5401 ccgccgatga acatcaccat agtcgcccct tattgctcgc tgccgtccga accgtatttc
     5461 aaccgctttt ggtatctggc agaacgattg tcgcaatcgc acgacgtgtt gctgattacc
     5521 agccgtttcc gccactacga caaatcattc agacggcatg aagatgctgc cgccacttca
     5581 aacggcaggc tgcgcgtcaa gctgctggac gaacctggct accgaaaaaa cgtctccctc
     5641 gcccgcgtcg ccagccaccg cgtattcgtc cgcaatttgg cacgctggct gcacagcccg
     5701 caggcggcgg aacaggacat cgtctattct gcctatccgc tgatggcgac caacctgctg
     5761 ttgggcaaac acaaagcgcg tttgggctac aaactgattg tcgatgtgca ggacgtatgg
     5821 ccggagtctt tctcctcggt cgtaccattt ttgaaaaaag taccgcacaa gctgctgccc
     5881 tttgcttcac gcgccaaccg tgcctaccgc tgcgccgacg cgctggttgc cgtatcgcag
     5941 acttatctcg accgcgccaa agaagccaat ccgaacgtac ccggcgaagc cgtctatatc
     6001 ggtacggatt ttgccgccat cgcccccccc cgcgattccg ctccaaaacc acccgccttt
     6061 tctacttggg aacgctcagt tacagctacg acgtggaaac cgtgtgcaaa ggtgttcgga
     6121 aacttttaga cgacggcgaa aacgtcgaat tgcacattat gggcggagga cccgatttgg
     6181 agaaactcaa acaatatgaa aatcgcgcca tcaagtttta cggttattta ccctattctg
     6241 aaatgatgtc gattgccaaa ggctgcgaca ttgccgtcaa cgccatccat agccacgcca
     6301 tgcagtcggt taccaacaaa ctctccgact atatggcttt gcaaaaaccg attctgaaca
     6361 gccagaccaa tgccgaagtc cttagcctct tgaacctgct gccgcacgaa aactaccgtt
     6421 ccggcgacgt ggacagcttc gttcaagccg ccaaagatat tttgaaacgc aaaaacgacc
     6481 ccgttcagtc cgatgaaatc gtccgccgct tcaggcgcga catttcctat cggaaaatcg
     6541 tcaacctgat tgaaagattg gcaaatgagt aaagccgtca aacgcctgtt cgacatcatc
     6601 gcatccgcat cggggctgat tgtcctctca cccgttttcc tgattttggc gtacctcatc
     6661 cgcaagaatc tgggttcgcc cgtcttcttc attcaagaac gccccggaaa ggacggaaaa
     6721 ccttttaaaa tggtcaaatt ccgttccatg cgcgacgcgc ttgattcaga cggcattccg
     6781 ctgcccgaca gcgagcggct cacacccttc ggcaaaaaac tgcgtgccgc tagtttggac
     6841 gaactgcctg aattatggaa tgtcttaaaa ggcgacatga gcctggtcgg cccgcgcccg
     6901 ctgctgatgc aatatctgcc gctgtacgac aacttccaaa accgccgcca cgaaatgaaa
     6961 cccggcatta ccggctgggc gcaggtcaac gggcgcaacg cgctttcgtg ggacgaaaaa
     7021 ttcgcctgcg atgtttggta tatcgaccac ttcagcctgt gcctcgacat caaaatccta
     7081 ctgctgacgg ttaaaaaagt attaatcaag gaagggattt ccgcacaggg cgaagccacc
     7141 atgccccctt tcacaggaaa acgcaaactc gccgtcgtcg gtgcgggcgg acacggaaaa
     7201 gtcgttgccg accttgccgc cgcactcggc cggtacaggg aaatcgtttt tctggacgac
     7261 cgcgcacaag gcagcgtcaa cggcttttcc gtcatcggca cgacgctgct gcttgaaaac
     7321 agtttatcgc ccgaacaata cgacgtcgcc gtcgccgtcg gcaacaaccg catccgccgc
     7381 caaatcgccg aaaaagccgc cgcgctcggc ttcgccctgc ccgttctggt tcatccggac
     7441 gcgaccgtct cgccttctgc aacagtcgga caaggcagcg tcgttatggc gaaagccgtc
     7501 gtacaggcag gcagcgtatt gaaagacggc gtgattgtga acactgccgc caccgtcgat
     7561 cacgactgcc tgcttaacgc tttcgtccac atcagcccag gcgcgcacct gtcgggcaac
     7621 acgcatatcg gcgaagaaag ctggataggc acgggcgcgt gcagccgcca gcagatccgt
     7681 atcggcagcc gcgcaaccat tggagcgggc gcagtcgtcg tacgcgacgt ttcagacggc
     7741 atgaccgtcg cgggcaatcc ggcaaagccg ctgccgcgca aaaaccccaa gaccgggacg
     7801 gcataagcg
//
LOCUS       DAWHRA010000093         7759 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWHRA010000093 DAWHRA010000000
VERSION     DAWHRA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.093"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..358)
                     /locus_tag="WIB30_001519"
     CDS             complement(<1..358)
                     /locus_tag="WIB30_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEZ3797568.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARF"
     gene            complement(499..1419)
                     /gene="ribF"
                     /locus_tag="WIB30_001520"
     CDS             complement(499..1419)
                     /gene="ribF"
                     /locus_tag="WIB30_001520"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ3797569.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIAADMEAAKCW"
     gene            complement(1718..3013)
                     /gene="tyrS"
                     /locus_tag="WIB30_001521"
     CDS             complement(1718..3013)
                     /gene="tyrS"
                     /locus_tag="WIB30_001521"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ3797570.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLYQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEVLVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(3076..4854)
                     /locus_tag="WIB30_001522"
     CDS             complement(3076..4854)
                     /locus_tag="WIB30_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ3797571.1"
                     /translation="MAAGRVSRCRYIFRDFRLFDDDDSSPRNVGGGRFSLKAFYIRRI
                     KRILPAFFAVLAATLIGGFFLFTKDDFFLLWKSALTALGFASNLYFARGKDYFDPAQE
                     EKPLLHIWSLSVEEQFYFVFPILLLLVARKSPRVQFGFLAALCALSLAASFMPSAFDK
                     YYLPHLRACEMLVGSLTAVWMRCRQPAVGRRCAAAGALFSACILSACLFAYSKQTAYF
                     PGPAALIPCLAVAALIYFNHYEHPLKKFFQWKITVAAGLISYSLYLWHWPILAFMRYI
                     GPDNLPPYSTAAAIVLILLLSLISYHCIEKPFKKWKGSFAQSVLWIYALPMLILGAGS
                     FFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELLVLGDSHADHYKTFF
                     DAVGKKEKWSATMVSADACAYVEGYASRVFQNWAACRAVYRYAEEHLPLYSKVVLAMR
                     WGSQMPENSRSLAYDAGFFQKFDRMLHKLSSEKQAVYLMADNLASSYNVQRAYILSSR
                     IPGYRQTLRPDDESTLKANARIRELAAKYPNVYIIDAAAYIPANFQIGGLPVYSDKDH
                     INPYGGTELAKRFSEKQRFLDTRHNH"
     gene            complement(4954..5058)
                     /locus_tag="WIB30_001523"
                     /pseudo
     CDS             complement(4954..5058)
                     /locus_tag="WIB30_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(5074..5280)
                     /locus_tag="WIB30_001524"
                     /pseudo
     CDS             complement(5074..5280)
                     /locus_tag="WIB30_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5318..6409)
                     /gene="ychF"
                     /locus_tag="WIB30_001525"
     CDS             complement(5318..6409)
                     /gene="ychF"
                     /locus_tag="WIB30_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ3797572.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(6522..>7759)
                     /locus_tag="WIB30_001526"
     CDS             complement(6522..>7759)
                     /locus_tag="WIB30_001526"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ3797573.1"
                     /translation="HIYQGNELNIDPKRVLWRRVVDMNDRQLRNIIDGMGKPVDGVMR
                     PDGFDITVASEVMAVFCLAKDIGDLKERLGNILVAYAKDGSPVYAKDLKAHGAMAALL
                     KDAIKPNLVQTIEGTPAFVHGGPFANIAHGCNSVTATRLAKHLADYAVTEAGFGADLG
                     AEKFCDIKCRLAGLKPDAAVVVATVRALKYNGGVERANLGEENLDALEKGLPNLLKHI
                     SNLKNVFGLPVVVALNRFVSDSDAELAMIEKACAEHGVEVSLTEVWGKGGAGGADLAR
                     KVVNAIESQTNNFGFAYDVELGIKDKIRAIAQKVYGAEDVDFSAEASAEIASLEKLGL
                     DKMPICMAKTQYSLSDNAKLLGCPEGFRIAVRGITVSAGAGFIVALCGNMMKMPGLPK
                     VPAAEKIDVDEHGVIHGLF"
BASE COUNT         1701 a         1983 c         2236 g         1839 t
ORIGIN      
        1 ggaaacgcgc tttgggcatg tctttgccgt gcagtttttc taccatcact tcgatgggca
       61 ggccgtggca gtcccaaccc ggcacataag gcgcgtcaaa accggcttgg gttttgctgc
      121 ggataatgat gtctttgaga attttattga cggcatgacc gatgtggatg tcgccgttgg
      181 catacggcgg gccgtcgtgc aggatgaatt tcggacggcc tttggcgatt tcgcgcagtt
      241 tttggtagcg tttttgctcg taccagcttt tcagccatgc aggctcgcgc ttggcaagat
      301 tgccgcgcat cgggaacggg ctttcgagca ggttgacggt tttgctgtaa tcggtcattt
      361 tttaatctct attgttacaa tatttcggtt cagacggcat tgcgcctcaa acagtatttc
      421 acaacgggaa acccctatgc cgtctgaaca gttaaaagat tgattgtagc ccaatcggat
      481 ggtttgtata aggtttttct accaacactt tgcggcttcc atatcggctg ctatctgcct
      541 tttcagttct gccataccgt caaacttttc ttcatcgcgc agtttgtgca ggaagcggac
      601 gttcagccgt tgtccgtaca ggtcgccttg aaagtcgaac aggtggactt caagcttttg
      661 agaacagccg ctatcaacgg tgggattgaa accgaagctc gccacgccgc gccgcgtgcc
      721 gaatgcgccg tccgcttcga cgacgaacac gccgccgagt gcataacggt ggcggggcag
      781 gcggatgttg gcagtcgggg cgtttaaggt gcgtccgagt tttctgccgt gcaccaccct
      841 gccgctcaag acgtagtcgt gtcccaaaag ttttttcgca taggcaaggt tgccgtctga
      901 aagggcttgt cgcacggcgg tactgctggt gcggatgtct tcgacgatga cggaaggcgt
      961 acgctcggtc tgcatatcgg gctgttgtgc caaaagttca aaacagcctt cccgccccgc
     1021 accgaaacgg aaatcatcgc cgacgagcaa ataacgcgta ttcaaggttt gacgcagcag
     1081 gcggtcgata aacccttgcg cggatatttc ggaaaaattt tgatcgaaac gcaaaaccca
     1141 gacggcatcg acacagcctg tgccttccaa taattcgagc ttggtgcgca gggggctgat
     1201 ccgacacggt ggcatcctgc cggtgcggag tgcgaaaaat tctttgggtt ggggttcgaa
     1261 aacgacggtc acgacgggta atccgcgcgt atcggcttcg aggcggagtt tttggaggat
     1321 gtgtttgtgt ccaaggtgta cgccgtcgaa attgcctatg gttacggcgg ccccgtgcgg
     1381 aaagtcgggc gcgttgtgcc gccccagcct gattctcatt gttgcgttcg ggtatgtggt
     1441 gaaacaggcg gtcattgtaa acggtattgc ggtttataga cagtgtgccg cccgttacgc
     1501 ccgcccgacg ctggaaaagc aggcaaattt cccgccgccg aacgcgccaa acgcataaag
     1561 cgtgcatcag gcgcgatcct atgtgttgaa acatcgcccc aaacctgatg caacatcagt
     1621 gaaaatcgtt cttttttaac caggtaaacc gagacaaaca accctccgcc gtcattcccg
     1681 cgcaggcggg aatgacggca tttctgcggc aatcggatta tttccaaacc aaaagcgcgt
     1741 ggttgcgttt gccgcgccga aggatggtgt atttgccgaa acgtttgtgt tcgtcggtca
     1801 gcaggcaggc atcgtcgggg cgttcggcgg cgtggttggg gttgttggct tcggcaggtt
     1861 tgccgttgag caaaaccgct ttgctgttca caaagccgcg cgcttcttta ttggaggatg
     1921 ccaaaccggt ttttaccaag acttcgacga cattgatgcc gtctgaaact tcaaatgcag
     1981 gcagaccgtc gagggcgagc tgctcgaagt cgctttcggt caggctgctt tggtcttcgg
     2041 caaacaggct ttcggaaatg cgttgcgcgg cggcaagggc ttcttcgccg tgaatcaggc
     2101 gggtcatttc ttcggcgagg atgcgttgcg cttcgggctt gctgccgctg gctttatctt
     2161 tcgcttcgat agcgtcgatt tcttcgatgg acaggaaggt aaagtatttc aggaatttat
     2221 acacatcggc atcggcgact ttcagccaga attggtagaa ctgatagggc gaggtttttt
     2281 tcgcgttcag ccacaccgcg ccgccttcgg ttttgccgaa tttggtgccg tctgatttgg
     2341 taaccaaagg cagagtcagt ccgaacactt gtttttggta caggcggcgg gtcaggtcga
     2401 taccggcagt gatgttaccc cattggtcgg agccgccgat ttccaaaacc gcgccgtggc
     2461 gtttgttcaa ctcggcgaag tcgtaacctt gcagcaggga ataggcgaac tcggtgaagg
     2521 aaatgcctgc gccgtcgcgg tcgatgcgct gtttgacgga ttctttgttc agcatggcgt
     2581 tgacggagaa atgcttgccg atgtcgcgca ggaagtcgag gcagttcatt ttgccgaacc
     2641 agtcggcgtt gttcgccata atggcggcgt ttccgccttc aaagctcaag aaaggggtta
     2701 attggctgcg gatgctttcc acccagccgg caacggtttc ggcggaattc aagctgcgtt
     2761 cggcggcttt gaagctgggg tcgccgatca taccggtcgc accgcccacc agtgcaatcg
     2821 gcgtatgccc cgcctgttgg aagcggcgca atgccaatac gggcagcagg tgtccgatgt
     2881 gcaggctgtc ggcggtcggg tcgaagccgc aataaaggga aattttttgt tcgttcaaca
     2941 aagcgtctaa ggcttcgatg tcggtggttt gcgcgattag gccgcgcgat tgcaggtctt
     3001 ggatgacgct catcggtctc tttcaaaaaa aattagcggt tttgcaaacc gccgattgta
     3061 acaaatttaa gcgaatcaat ggttatggcg cgtatcgaga aaccgttgtt tttcggaaaa
     3121 acgcttcgcc aattccgtgc cgccgtaagg gttgatgtgg tctttgtccg agtaaaccgg
     3181 caatccgccg atttggaaat ttgcggggat ataggcggcg gcatcaataa tatagacgtt
     3241 ggggtatttg gctgccaatt ccctgatgcg tgcattggct ttcagagtgc tttcgtcgtc
     3301 cgggcgcagg gtttggcggt aacccggtat gcgtgaagac aagatatagg cgcgttgtac
     3361 gttgtaagac gaggcaaggt tgtccgccat caggtaaacg gcttgttttt cggatgagag
     3421 tttgtgcagc atacggtcga atttttggaa aaaaccggca tcgtaggcaa gggagcggct
     3481 gttttcgggc atttggctgc cccagcgcat cgccaaaacc acttttgaat acaggggcag
     3541 gtgttcttcg gcatagcggt aaacggcgcg gcaggcggcc cagttttgga acacacggga
     3601 cgcgtagcct tccacatagg cgcaagcgtc ggcggaaacc atagtggcgg accatttttc
     3661 ttttttgccc acggcatcga agaatgtttt gtaatggtcg gcgtgggagt cgcccaaaac
     3721 cagcagctcc ggctgttttt ccgtatcccc ccacaggcat tgtttgccgg tattgttgtg
     3781 gcaggaggtg ttggaacgcg tcagccccaa gcggtcgtat tgcgccataa acggcagcct
     3841 catcgcgaaa aacgagcccg cccccaaaat gagcataggc aaggcataaa tccataaaac
     3901 ggattgtgcg aacgaacctt tccatttttt aaacggcttt tcgatgcagt gataagaaat
     3961 cagggaaagc agcaatatca ggacgattgc tgccgctgtt gaataaggcg gcaggttgtc
     4021 cgggccgata tagcgcataa aggccaatat cggccaatgc cacagataaa gcgaatagga
     4081 aatcaaaccg gcggcaacag tgattttcca ttggaaaaat tttttaagcg ggtgttcgta
     4141 atgattgaaa taaatcagcg cggcaacagc cagacaggga atcaaagcgg cgggtcccgg
     4201 gaaataggcg gtttgtttcg aataggcaaa caggcaggcg gacagtatgc acgcggaaaa
     4261 caatgcgccg gcggcggcac agcgtctgcc gacggcaggt tgccggcagc gcatccacac
     4321 ggcggtcagc gatccgacca gcatttcgca ggcgcgcagg tggggcaggt aatatttatc
     4381 gaacgcggaa ggcataaagg aagcggcaag gcttaaggcg cacaatgcgg cgaggaagcc
     4441 gaactgtacg cgcgggcttt tgcgggcgac aagcagcagc aatatcggaa agacaaagta
     4501 aaattgttct tcgaccgaca aagaccaaat gtgcagcaag ggcttttctt cctgcgcggg
     4561 atcgaaataa tccttccccc ttgcaaaata caggttggag gcgaaaccca aggcggtcag
     4621 cgcggatttc cacaaaagaa agaaatcatc tttggtgaat aaaaagaagc cgcctatcag
     4681 cgttgccgcc aatacggcga aaaatgcggg caaaatccgt ttgatgcggc ggatataaaa
     4741 tgccttcagg gaaaacctcc cccccccgac atttcgcggt gaagaatcat cgtcatcaaa
     4801 aagcctgaaa tcacgaaaaa tatatcgaca ccgagaaacc cgcccggcag ccaatttttt
     4861 tcgatatgga acacgatgac ggacaagacg gcggcggcgc gcaatgtgtc gatgtccggg
     4921 cggtagggta aggcttggct cataatgttt ttatagtgga ttaaatttaa accagtacgg
     4981 cgttgcctcg ccttgccgta ctatctgtac tgtctgcggt ttcgtcgcct tgtcctgatt
     5041 taaagttaat ccactatact cgaaacgcgg cggcgcaaat gccgtctgaa aggtcatttt
     5101 gtatcggggc tcgggatatt cggaatgccg gacggctttt tgcaacagcg gggcaggcgg
     5161 tttgttttgc aggaatcggg gagggcaaat cggaaatgcg gacgggattt tattttgatg
     5221 cagccgcatt ccggcggtat ggaaacgccg aaaaccacca aaaccggctt cagacggcat
     5281 ttctggcaaa gccgtctgaa aacctgccgc atttgggtta cacgttaaac aaaaagtgca
     5341 tcacatcccc atcctgcacg acgtattcct taccttccac acgcattttg ccggcttctt
     5401 tggctttggc ttcgccgccg agcgagacaa agtcgtcgta agaaatgact tgggcgcgga
     5461 tgaagccgcg ttcgaagtcg gtgtgaatca cgccggcggc ttgtggggct gtgtcgcctt
     5521 tgtgtatcgt ccacgcgcgg acttctttga cgccggcagt gaagtaggtt tgcagcccca
     5581 aaaggtcgta accggcgcgg atcaggcggt tcaggcccgg ttcttccaag cccatttcgg
     5641 cgaggaactc ggctttttca ccgtcttcca actcggcgat ttcgctctcc atcgcggcgc
     5701 acacggcgac gacgggggcg ttttcttttg ccgccaattc tttcaggcgg tcgaggtgcg
     5761 ggttgttttc aaaaccgtct tcggaaacgt tgccgacgta catggcaggt ttggcggtca
     5821 gcaggaacag cggtttgagc atcgcgcgtt cttccgcgtc caaaccgaag gaacgcacgg
     5881 gtttgccttc gtccagatgc ggcagcagtt ttttgcacaa atcgaccagc ttttgcgcgt
     5941 ctttgtcacc ggaacgggcg cgtttttctt cgcggacgat ggctttttcc acacttgcca
     6001 agtcggcaag cgccaactct gtaccgatgg tttcgatgtc ggcaatcgga tcgacgcggc
     6061 ctgcaacgtg gacgatgttg tcgtcgtcaa agcagcgcac gacattcaca atcgcatcgg
     6121 tttcgcggat gttggcaagg aactggttgc ccaagccctc gcctttgctc gcgcctgcaa
     6181 ccaaaccggc aatatcgaca aattcgacga tggcaggctg cattttttgc ggattgacga
     6241 tttttgccaa ttcggccata cgcggatcgg ggacttcgac gatgccgacg ttgggttcga
     6301 tggtacagaa aggatagttt gccgcttcga tacccgattg ggtcagcgcg ttaaaaaggg
     6361 tggatttgcc gacgttgggc aaaccgacga tgccgcattt caaactcatg ttttttcctg
     6421 aaaatagaga aatttaacgg cggattatag cataccgccg cccgcgttcc gaaaaatgcc
     6481 gtctgaaacg gcttcagaca gcatccggtt tcaaaaaccg ttcagaacaa gccgtgaatc
     6541 acgccgtgtt cgtccacatc gattttctcg gcggccggaa ctttcggcag acccggcatc
     6601 ttcatcatat tgccgcacag cgcgacgatg aagcccgcgc cggcggaaac ggtgatgccg
     6661 cgcacggcga tgcggaagcc ttcggggcag cccaacagtt tggcgttgtc actcaaagag
     6721 tattgggttt tcgccatgca gatcggcatt ttgtccaagc ccagtttttc cagtgaagcg
     6781 atttcggcag acgcttccgc gctgaaatca acatcttccg cgccgtacac tttttgggca
     6841 atcgcacgga ttttgtcttt gatgcccaac tcgacatcgt aggcgaaacc gaagttattg
     6901 gtttgacttt caatggcgtt gacgactttg cgcgccaaat ccgcgccgcc cgcaccacct
     6961 ttgccccaca cttcggtcag ggaaacttca acgccgtgtt cggcacaggc tttttcaatc
     7021 atcgccaact cggcatcgga gtcggacacg aagcggttga gcgcaacgac gacgggcagt
     7081 ccgaatacgt ttttcaggtt ggaaatgtgt ttcagcaggt tgggcaaacc tttttccaaa
     7141 gcgtctaaat tttcttcgcc gaggttggcg cgttccacgc cgccgttata tttcaacgcg
     7201 cggacagtcg ccacgacaac agccgcatca ggtttcaaac cggcaaggcg gcatttgatg
     7261 tcgcagaatt tttccgcgcc caagtccgcg ccgaagcctg cttcggttac ggcgtaatcg
     7321 gcaaggtgtt tcgccagacg ggttgcggtt acggagttgc agccgtgggc gatgttggcg
     7381 aacgggccgc cgtgtacgaa ggcgggcgtg ccttcgatgg tttgcaccaa gttaggctta
     7441 atcgcatctt tgagcagggc cgccatcgcg ccgtgtgcct tcaaatcttt ggcgtaaacg
     7501 gggctgccgt ctttggcgta ggcgacaagg atgttgccca aacgctcttt caaatcgccg
     7561 atgtctttgg cgagacagaa taccgccatc acttcggagg caacggtaat gtcgaaaccg
     7621 tcggggcgca tcacaccgtc aacaggttta cccatgccgt cgatgatgtt gcgcaactgg
     7681 cggtcgttca tatcgaccac gcgccgccac agcacgcgtt tggggtcgat gttcaactcg
     7741 ttgccttggt agatatggt
//
LOCUS       DAWHRA010000094         7719 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWHRA010000094 DAWHRA010000000
VERSION     DAWHRA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.094"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            332..535
                     /locus_tag="WIB30_001527"
     CDS             332..535
                     /locus_tag="WIB30_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797574.1"
                     /translation="MPSEAAWAFRRHFLRLKQHIGNPAPPVHGNLHLKTICMPNHTPI
                     PISVICTLPVLGNHRFFAYNSRI"
     gene            562..1524
                     /locus_tag="WIB30_001528"
     CDS             562..1524
                     /locus_tag="WIB30_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ3797575.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            1571..1942
                     /locus_tag="WIB30_001529"
     CDS             1571..1942
                     /locus_tag="WIB30_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ3797576.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            1968..2894
                     /gene="fabD"
                     /locus_tag="WIB30_001530"
     CDS             1968..2894
                     /gene="fabD"
                     /locus_tag="WIB30_001530"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ3797577.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAGHAVVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFTDAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESAQDEVVEAVNFN
                     SPGQVVIAGNAAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDVDKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAVCSALTDAGQVAAFIEAH"
     gene            3032..4897
                     /gene="msbA"
                     /locus_tag="WIB30_001531"
     CDS             3032..4897
                     /gene="msbA"
                     /locus_tag="WIB30_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ3797578.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLFALQTANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            4997..6562
                     /gene="guaA"
                     /locus_tag="WIB30_001532"
     CDS             4997..6562
                     /gene="guaA"
                     /locus_tag="WIB30_001532"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ3797579.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNESKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVIALRAVITSDFMTAHWAELPYSLLGKVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            6624..7370
                     /gene="fabG"
                     /locus_tag="WIB30_001533"
     CDS             6624..7370
                     /gene="fabG"
                     /locus_tag="WIB30_001533"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ3797580.1"
                     /translation="MSTQDLNGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETIESLIADIEKAFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1866 a         2394 c         1877 g         1582 t
ORIGIN      
        1 atcgacggtc gtttttggcg gattttttat ggcagacaac ggagagcgaa tccaaatccc
       61 cgttttggaa aatcctgaca ttagggaaat caatcacttt tttccgtatc aaattttgag
      121 aaaaaagccg gcgtccttgt tttcagaatc atccccgagc cggaatttgg cgataccgaa
      181 ttaactgtct attttgaaaa ggatattata gtggattaac aaaaaccagt acggcgttgc
      241 ctcgccttgc cgtactggtt tttgttaatc cactatatca gacgaaaaca aacacccgcg
      301 ccaatagcct gacggcaacc cggcaatcaa aatgccgtct gaagcagctt gggctttcag
      361 acggcatttc ctccgcttaa aacagcatat cggcaacccc gccccgcccg tccacggcaa
      421 tctgcatctg aaaaccatct gtatgccaaa ccacaccccc atcccgattt ccgtcatttg
      481 caccctgcct gtattgggca atcatcggtt tttcgcttac aatagccgaa tctgaaccaa
      541 ctatccaaaa aggccgttcc catgcagtat gccaaaattt ccggcacggg cagctatctt
      601 cctgccaacc gcgtcagcaa tgacgacctt gcccaaaaag tggatacctc ggacgagtgg
      661 attaccgcgc gcacgggcat caaattccgc catattgcag ccgaaaacga aaaaaccagc
      721 gaccttgccg ccgaagcggc gcggcgcgct ttggcagatg ccaaattaaa tataaacgac
      781 attgatttga ttatcgtggc gacggcaacg ccggatatgc agtttccttc taccgccacc
      841 atcgtgcagc aaaaattggg catcaccaac ggctgtcccg cgtttgacgt acaggcagtg
      901 tgcgcaggct ttatgtacgc gctgaccacg gcaaacgcct acattaaaag cggtatggcg
      961 aaaaacgcgc tggtcatcgg cgcggaaacc ttcagccgca tcgtcgattg gaacgaccgc
     1021 acaacctgcg tattgttcgg cgacggcgcg ggcgcggtgg ttttgagcgc ggcggacaaa
     1081 ccgggcatca tccacagcaa actcaaagcc gacggcaatt atctgaaact cttaaacgtc
     1141 cccgggcaaa tcgcctgcgg caaagtttcc ggttcgccgt acatttcgat ggacggtccc
     1201 ggcgtgttca agtttgccgt caaaatgctg tccaaaatcg ccgatgacgt tatcgaagaa
     1261 gccggttata ccgccgctca aatcgactgg atcgtgccgc atcaggcaaa ccgccgcatt
     1321 atcgaatcga ccgcgaaaca tttaggtttg agtatggaca aagtcgtcct gaccgtccaa
     1381 gaccacggca acacatccgc cgcatcgatt ccgctggctt tggatacggg catccgcagc
     1441 ggacaaatca aacgcggtca aaacctgctg ctcgaaggca tcggcggcgg tttcgcgtgg
     1501 ggcgcggtgc tgttgcaata ttgaacccga tgccgtctga aacaggcttt cagacggcat
     1561 ttcccatatc atgaagcggc aggctttctt caaactgatg gcgtgtgcgg catttctgtc
     1621 tgccgtttcg ctgcgcctcc ccgtattggg cgcgtgttac gcaatattgt ccctctatgc
     1681 gtttgcactt tacggcatcg acaaacggcg tgccgtgcga ggaaaacgcc gcattcccga
     1741 acaccgcctg ctgctgcctg ccttgttcgg cggttggacg ggcgcatact tgggcagcag
     1801 gatattcagg cataaaacgg cgaaaaagcg ttttgttgtg ctgttccgtc tgactgtttc
     1861 gggcaatgtc ctagcgacct gcatcctgat tgattatttc gttccgcccg aactttttgt
     1921 aaaactcggg caacatctct gacaatccaa cccaacaaag gaatactatg tcttttgcct
     1981 tcttttttcc cggacaaggt tcccaaagcc tcggtatgat gaacggcttt gccggacacg
     2041 ccgtcgtcaa aaacaccttt gccgaagcct ccgccatatt ggggcaggac ttgtgggcga
     2101 tgattaacgg cagcgatgcc gaaatcatcg gtcaaaccgt caacacccag cccattatgc
     2161 tcgccgccgg cgttgccgtt taccgcgcct atttggaagc gggcggcaaa acgcctgccg
     2221 ccgttgccgg acacagcctc ggcgaataca ccgcactcgt tgccgccggc gcattggatt
     2281 ttaccgatgc cgtcaaactc gtgcgcctgc gcgccgaact gatgcagtcc gccgtaccgc
     2341 aaggcgtggg cgcaatggcg gcgattctcg gcttggaaga tgaacaggta aaacaaattt
     2401 gcgccgaatc cgcacaggat gaagtggttg aagccgtcaa cttcaactcg cccggacaag
     2461 tcgtgattgc aggcaatgcc gccgccgtcg agcgcgctat gcttgccgcc aaagaagccg
     2521 gcgccaaacg cgccctgccg ctgcccgtgt ccgtaccttc ccattgcagc ctgatgaaac
     2581 ccgccgccga caaacttgcc gaagccctga aaaccgttga aatcaagcag ccgcaaatcc
     2641 gcgtcatcca caacgccgac gttgccgcct acgatgatgt cgacaaaatc aaagacgcgc
     2701 tcgtccgcca gctttacagc cccgtacgct ggacggaaac cgtcaacgcc ctcgtttcag
     2761 acggcattgc cgaatccgcc gaatgcggcc cgggcaaagt gttggcgggc ttggcaaaac
     2821 gcatcaacaa agccgccgtg tgcagcgcgc tgaccgatgc cggacaggtt gccgccttta
     2881 tcgaagcgca ctgacttcgt tccgcaaaaa gcagcctgcc ctcttcaggc tgcttttcac
     2941 gtccgaacgg cggcagcccc atatttacgc tataatccat cccgaccaaa ccaccgacag
     3001 cggctgccgt tgcagttccc gccccaccga tatgatagaa aaactgactt tcggactgtt
     3061 taaaaaagaa gacgcgcgca gctttatgcg cctgatggca tacgtccgcc cctacaaaat
     3121 ccgcatcatt gccgccctga ttgccatttt cggcgttgcc gccaccgaaa gctaccttgc
     3181 cgccttcatc gcccccctga tcaaccacgg cttttccgcg cccgccgcgc cgcccgagct
     3241 gtctgccgcc gccggcatca tttccaccct gcaaaactgg cgcgaacagt ttacctatat
     3301 ggtttggggg acggaaaaca aaatctggac cgtcccgctc ttcctcatca tcctcgtcgt
     3361 catccgtggc atctgccgct ttaccagcac ctatctgatg acttgggtct ccgtgatgac
     3421 catcagcaaa atccgcaaag atatgtttgc caaaatgctg accctttcct cccgctacca
     3481 tcaggaaacg ccgtccggca ccgtactgat gaatatgctc aacctgaccg aacagtcggt
     3541 cagcaacgcc agcgacatct tcaccgtcct cacgcgcgac acgatgatcg ttaccggcct
     3601 gaccatcgtc ctgctttacc tcaactggca gctcagcctc atcgtcgtcc tgatgttccc
     3661 cctgctctcc ctgctctcgc gctactaccg cgaccgtctg aaacacgtca tttccgactc
     3721 gcaaaaaagc ataggcacga tgaacaacgt gattgccgaa acccatcagg gacaccgcgt
     3781 cgtcaagctg ttcaacgggc aggcgcaggc ggcaaaccgg ttcgacgcgg tcaaccgcac
     3841 catcgtccgc ctcagcaaaa aaatcacgca ggcaacggcg gcacattccc cgttcagcga
     3901 actgatcgcc tcgatcgccc tcgccgtcgt catcttcatc gccctgtggc aaagccaaaa
     3961 cggctacacc accatcggcg aatttatggc attcatcgtc gcgatgctgc aaatgtacgc
     4021 ccccatcaaa agccttgcca acatcagcat ccctatgcag acgatgttcc tcgccgccga
     4081 cggtgtatgt gcatttctcg acaccccgcc cgaacaggac aagggcacgc tcgcaccgca
     4141 gcgtgtcgaa gggcgcatca gcttccgcaa cgtcgatgtc gaataccgtt cagacggcat
     4201 caaagccctc gacaacttca acctcgacat cagacaaggc gaacgcgtcg ccctggtcgg
     4261 acgttccggc agcggcaaat ccaccgtcgt caacctgctg ccccgctttg tcgaaccgtc
     4321 tgccggcaac atctgcatag acggtatcga catcgccgac atcaaactcg actgcctgcg
     4381 cgcccaattc gccctcgtct cccaagacgt attcctgttt gacgacaccc tgtttgaaaa
     4441 cgtccgatac agccgtcccg acgcgggcga agccgaagtc ctgttcgccc tccaaaccgc
     4501 caacctgcaa agcctgattg acagctcccc gctcggactg caccagccca tcggatcgaa
     4561 cggcagcaac ttatccggcg gacagcggca acgcgtcgcc attgcccgcg ccattttgaa
     4621 agacgcgccg atattattat tggacgaagc caccagcgca ttagacaacg aatccgaacg
     4681 cctcgtccaa caggcgctcg aacgcctgat ggaaaaccgc accggcatca tcgtcgccca
     4741 ccgcctgacc accatcgaag gggccgaccg catcatcgtg atggacgacg gcaaaatcat
     4801 cgaacaaggc acacacgaac aactgatgtc ccaaaacggt tactacacga tgttacgcaa
     4861 tatctcaaac aaagatgccg ccgtccggac ggcataaaca aaatgccgtc cgaaatggta
     4921 caatcgcccc gaccctttca gacggcatca tatccgccga cccatccgat tatcttcaat
     4981 cactgtaaaa cccattatga cccaagacaa aatcctcatc cttgacttcg gttcgcaagt
     5041 tacccagctc atcgcccgcc gcgtgcgcga agcccacgtt tactgcgagc tgcattcttt
     5101 cgatatgcct ttggacgaaa tcaaagcctt caaccccaaa ggcatcatcc tctccggcgg
     5161 ccccaattcc gtttacgaat ccgactatca agccgatacc ggtatttttg atttgggcat
     5221 tccggttttg ggcatctgtt acggcatgca gtttatggcg caccacttgg gcggcgaagt
     5281 gcagcccggc aaccagcgcg aattcggtta tgcgcaagtt aaaaccatag acagcgagct
     5341 gacacgcggc attcaagatg gtgagccaaa cacactcgac gtatggatga gccacggcga
     5401 caaagtgtcc aaactgcccg acggtttcgc cgtcatcggc aacaccccgt cctgcccgat
     5461 tgccatgatg gaaaacgccg aaaaacaatt ctacggcatc cagttccacc ccgaagttac
     5521 ccacaccaaa caaggccgcg ccctgttgaa ccgctttgtc ttggatattt gcggcgcaca
     5581 accgggctgg acgatgccga actacatcga agaagccgtt gccaaaatcc gcgaacaggt
     5641 cggcagcgac gaagtgattt taggtctgtc cggcggcgtg gactcttccg tagccgccgc
     5701 gctgattcac cgcgccatcg gcgaccaact gacctgcgtg ttcgtcgatc acggtttgtt
     5761 gcgcctgaac gaaagcaaaa tggtgatgga tatgttcgcc cgcaacttgg gtgtgaaagt
     5821 gatacacgtc gatgccgaag ggcagtttat ggcgaaactc gccggcgtaa ccgaccccga
     5881 gaaaaaacgc aaaatcatcg gtgcggaatt tatcgaagta tttgatgccg aagaaaaaaa
     5941 acttaccaac gccaaatggt tggcacaagg cacgatttac cctgacgtaa tcgaatccgc
     6001 aggtgcaaaa accaaaaaag cccacgccat caaatcgcac cacaacgtcg gcggcctgcc
     6061 tgaaaacatg aagctcaaat tgcttgagcc tttgcgcgat ttgttcaaag acgaagtacg
     6121 cgaattgggt gtggctttgg gcctgccgcg cgaaatggtg taccgtcatc cgttcccggg
     6181 tccgggtttg ggcgtgcgta ttttgggcga agtgaaaaaa gaatatgccg acctgcttcg
     6241 tcaggcagac gatattttca ttcaagaatt gcgcaatact accgatgaaa acggtacatc
     6301 ttggtacgac ctgaccagcc aggcattcgc cgtgttcctg cccgtcaaat ctgtcggcgt
     6361 aatgggcgac ggccgcacat acgattacgt cattgccttg cgtgccgtga ttaccagcga
     6421 ctttatgacc gcgcattggg cggaactgcc gtattccttg ttgggcaaag tgtccaaccg
     6481 catcatcaac gaagtcaaag gcatcaaccg cgtggtttat gatgtgagcg gcaaaccgcc
     6541 tgccaccatc gagtgggaat aaacagcaaa catggctgcc ccgtccggcg cagtccttcg
     6601 attatcggaa aaaaggaaaa aatatgagca cacaagattt aaacggcaaa atcgctttgg
     6661 taacaggcgc atcgcgcggt atcggtgcag caattgccga cacgctggcg gcagccggtg
     6721 ccaaagtcat cggtacggcg accagtgaga gcggtgcggc ggcgattagc gagcggttgg
     6781 cgcaatgggg cggcgaaggc cgcgtattaa attccgccga acctgaaacc atcgaaagcc
     6841 tgattgccga catcgaaaaa gcgttcggca aactcgatat tctggtcaac aacgccggca
     6901 tcacccgcga caacctcctg atgcgcatga aagaagaaga gtgggacgac atcatgcagg
     6961 tcaacctcaa atccgtgttc cgcgcttcta aagccgtttt gcgcggtatg atgaaacaac
     7021 gttccggccg catcatcaac atcacatccg tcgtcggcgt gatgggcaat gccggtcaaa
     7081 ccaactatgc cgcggcaaaa gcaggcttaa tcggtttctc caaatccatg gcgcgcgaag
     7141 tcggcagccg gggcattacc gtcaactgcg tcgcccctgg ctttatcgat accgacatga
     7201 cacgcgccct gccggaagaa acccgccaaa cctttaccgc ccaaaccgcc ttgggcagat
     7261 tcggcgacgc gcaagacatc gccgatgcgg ttctgttcct cgcttccgac caagcaaaat
     7321 acatcaccgg ccaaacgctg cacgtcaacg gcggtatgct gatgccttaa cagacaactt
     7381 tttcaaccat gccgtctgaa gccctttcag acggcatttg cattctcagg caaaatgaac
     7441 acacaccaca ccccgccctg cccatgcggc tcaggcacaa gctgagacct ttgcaaaatt
     7501 cctttccctc ccgacagccg aaaccccaac acaggttttc agctgttttc agctgttttc
     7561 gccccaaata ccgcctaatt ctacccaaat acccccttaa tcctccccgg acacctgata
     7621 atcaggcatc cgggtcacct tttaggcggc agcgggcgca cttagcctgt tggcggcttt
     7681 caaaaggttc aaacacatcg ccttcagatg gctttgcgc
//
LOCUS       DAWHRA010000095         7660 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWHRA010000095 DAWHRA010000000
VERSION     DAWHRA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.095"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(31..447)
                     /gene="dksA"
                     /locus_tag="WIB30_001534"
     CDS             complement(31..447)
                     /gene="dksA"
                     /locus_tag="WIB30_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ3797581.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(594..1385)
                     /locus_tag="WIB30_001535"
     CDS             complement(594..1385)
                     /locus_tag="WIB30_001535"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ3797582.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIYIANRGAEKRERLEKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNICTNGALVLSVAAGLSVDTLSRYLGG
                     SRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDCKIADRIMKSVGLTVWLEDEVQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1434..1841)
                     /locus_tag="WIB30_001536"
     CDS             complement(1434..1841)
                     /locus_tag="WIB30_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ3797583.1"
                     /translation="MKIRAIKYTAMAALLAFTVAGCQLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1841..2527)
                     /locus_tag="WIB30_001537"
     CDS             complement(1841..2527)
                     /locus_tag="WIB30_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ3797584.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYASGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2647..3690
                     /locus_tag="WIB30_001538"
     CDS             2647..3690
                     /locus_tag="WIB30_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ3797585.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3853..5079
                     /locus_tag="WIB30_001539"
     CDS             3853..5079
                     /locus_tag="WIB30_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ3797586.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5365..7515
                     /gene="yccS"
                     /locus_tag="WIB30_001540"
     CDS             5365..7515
                     /gene="yccS"
                     /locus_tag="WIB30_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ3797587.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKIAERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALNTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1889 a         2552 c         1667 g         1552 t
ORIGIN      
        1 gggactcaga cggcattttg tttgccgcca tcagtcggca aactgttttt tcatcctctc
       61 tcggcgttct tgggactcaa cagataaagt ggctgtcggg cgtgccagca gccgcttcag
      121 accgataggc tctcccgtat cggcacagaa tccataatcc ccttcatcaa tattgcggat
      181 ggtcgcctgt attttactga gaagttttcg ttcccgatcg cgggtacgga gttccaatgc
      241 gtactcttct tcctgtgtgg cacggtcggc aggatcgggg gctgattcgt gttcttggag
      301 atgccctgtc gtagcggaag cattttcgat gagttcgtct tgcattttta ccagcaattc
      361 gcggaaaaaa gccaaatggt cgtcattcat ataatcgtct tccggcccgc tccaattcaa
      421 aatatcttgt tctgtcagct ttgccataat gtatatcctc tcggttcacc gaaaaaaaca
      481 aagcaccaca aagtggcttt attcctttga tttttataag gcttattgcc taaggcgttt
      541 ttgaaaacgc gcatcataac acgttttgga ttggtttttt tattttcttt acattattga
      601 tattgccgtt ccatttcctg cgaacggcgc acacaggcac aaacgccctc acttatggct
      661 tcggcgacac gatgccgcct gaaggcttcc acggcttcgt gggttgtccc gccttttgac
      721 gttacatttt tttgaagctt ctcgaaatct tcacccgtct gctcggcaag ggcaaccgct
      781 cctttaaacg ttgccagact gagcgcgcgt gcttctgcca tatcaaaccc ttgtcggatg
      841 gcggcatttt gcaaagcatc cagcagataa aacacataag ccggcccgct gccgctgatg
      901 ccggtgatgc tgtgcatttg cacttcatct tccaaccaaa cggtcaaacc gacagatttc
      961 atgattcgat cggcaatctt gcagtctgtt tccgatactt ccgcttcggc atacataccg
     1021 gagacaccca gtcctatttt cccgggtgta ttcggcataa cccggacaat gcggcggctt
     1081 cccccgaggt aacggctgag cgtatcaacc gacaatccag ctgcaacaga aagcaccaat
     1141 gcgccgttgg tgcagatatt tttgcacgcg gcttccatat cctgcggttt gacggcaagg
     1201 attaaaacat cgtcggaatg aagctccggc agggttgccg aagtttcgac ccccaactct
     1261 ttttccaaac gttcgcgttt ttccgcaccc cgattggcta tatagatgcg gtaaccgcct
     1321 tgtttgacca atccgcccgc aacggcagcc gccatattgc cgccgccgag aaaataaaca
     1381 ttcattattt ctaccctatt caaaattacc tgactaaatt cattcccaat tttttaccat
     1441 cggacgcgct cggacagacc gttgcgccgc aacccctgct tttccaagca gtcaaatcct
     1501 tcctgtttga acgtctcaat caaaggtttg ccgtcacgcg tacgccaatc caacccgcag
     1561 gcttcaaact tccctatttt cctgtaaaag taagattgtt gtgcggattc atattcgttt
     1621 gccctgacgg aacgattgcc gtctgaaagc ggtatctcgt agtcctctaa agacggcgga
     1681 ctctcgccgc caatgtccca aaaatcgatg gcagccggtt ttctcggctt acaccagccg
     1741 gacagggacg aacactcata ccaacccgcc aactggcagc ctgcaaccgt aaatgcaagc
     1801 aacgcagcca ttgccgtata ttttattgcc cgaattttca tcagcccctt ttcccgaaaa
     1861 tcgcgctgcc gatacggacg tgtgtcgcac cgcactcaat ggcggcaggc atatcgtccg
     1921 acatccccat agacagcacg tctgccttaa cgccagccgc attgaggtcg gcaagcagtt
     1981 tccgcatcgt ttgaaattgc accttcaact ccgtttcact gctgttggct ttggcaacac
     2041 acatcagtcc gcgtacgacg atattcggca gcttcgccac ttccacagca agcgcgactg
     2101 cttcttcggg cgcgacaccg tgcttcaccg cctcgcccgc aatgttcacc tcaatacaca
     2161 cctgcaaagg cggcattgag gaaggacgtt gcccgctcag ccggacggcg gtctttagac
     2221 ggcatacggt atgcacccaa tgcgcgcgtt cggcgacaaa cttggttttg ttggactgca
     2281 catcgccgat gacgtgccac acgatgtcgg tcaaatccgc caactcttcc gttttgccgt
     2341 accactcctg aatatagttc tcgccgaaat cacgctgtcc tgaggcgtaa acttcgcgga
     2401 tgccgtctga agggaaagtc ttaccgacgg caatcaggct gacggaatgc ggcttccttc
     2461 ccgcctgcag aaccaatttt ccgatacggt cggacacctc ccgataacgt tcttgcaaca
     2521 ccgtcataga ttatccccta attaaaaatg attaaacagt tgaaacccct ccagtcaggg
     2581 cggtacaatc aaggttgtta gaaccattcc aaccaatcga aacattatac taaacagagc
     2641 cgcattatgc agattaccga cttactcgcc ttcggcgcca aaaacaaagc atccgacctt
     2701 cacctgagtt cgggcatatc ccctatgatt cgagttcacg gcgatatgcg gcgcatcaac
     2761 cttcccgaaa tgagcgcgga agaggtcggt aatatggtaa cttcggtaat gaacgaccac
     2821 cagcggaaaa tctaccagca aaacttggaa gtcgacttct cgttcgaact gcccaacgtc
     2881 gcccgattcc gcgtcaacgc cttcaacacc ggccgcggtc ccgccgccgt attccgcacc
     2941 attcccagca ccgtcttatc gctggaagaa ttgaaagccc cgagcatttt ccaaaaaatc
     3001 gcagaatcgc cgcgcggcat ggtattggtt accggcccta ccggttcggg caaatcgacc
     3061 acgcttgccg cgatgatcaa ctacatcaac gaaacccagc cggcacacat cctgaccatc
     3121 gaagacccga tcgaattcgt ccaccaaagc aaaaaatccc tgattaacca gcgcgagctg
     3181 caccagcaca ccctcagctt cgccaacgcg ctgagttccg cattgcgcga agaccccgac
     3241 gttatccttg tcggcgagat gcgcgacccc gaaaccatcg gcttggcact gaccgccgcc
     3301 gaaaccggac acttggtttt cggcacgctg cacacgaccg gcgcagcaaa aaccgtcgac
     3361 cgtatcgtgg acgtattccc ggcgggagaa aaagaaatgg tgcgctctat gctgtccgaa
     3421 tcgctgaccg ccgtcatctc ccaaaacctg ctgaaaacgc acgacggcaa cggccgtgtc
     3481 gcctcgcacg aaatcctgat tgccaacccc gccgtccgca acctcatccg cgaaaacaaa
     3541 atcacgcaga ttaactccgt cctgcaaacc gggcaggcga gcggtatgca gacaatggac
     3601 caatcgctgc aatcgctggt gcgccaaggg ctgatcgcac cggaagtcgc acgcagacgc
     3661 gcgcaaaaca gcgaaagtat gagtttctga cacacaaccg ccttccggcc ataccggcgg
     3721 gaaaacaagg cgcaaacacg cgaggcggga cgcagcatcc cgcccggcta cctttccgaa
     3781 caagaagcgt ccgccttcct gttgaaacct gccgccgcaa actgcaaggc ttaaaccgaa
     3841 aagaagctaa cgatgaatac cgataacctg cacgacatct tggacgaaat ggttcaagtg
     3901 tattctcaaa aaaaacaaag ccgatccgaa accccggccg aaatcggcgc acacttccac
     3961 ccgctgctcg accgcctgtg cgaaaccgca gaagcacaaa acgcgtccga catccttatc
     4021 agcaaaggat tcccgccctc gttgaaaatc aacagcgcat taaccccgca gccgcaaaag
     4081 gcgctgacag gcgaggaaac cgccgccatc gccgcatcga cgatgaacgc cgaacaatcg
     4141 gaaatattcc ggcgcgacgg cgaaatcaac tactccgtcc agtcgcgcag cggcacgcgc
     4201 taccgcgcca acgcctacca cagccaaggc agcgcaggtt tggttttgcg gcgcatcaac
     4261 cacgtcatcc cgcaaatgca ggaattgggc ctgcccgaaa aactcaaaga cctcgccgtc
     4321 gcaccgcgcg ggctgctgat tatcgtcggg cctaccggtt cgggcaaatc caccacgatg
     4381 gcgactatgc tcgaacaccg caacaaaacc ctgcccagcc atatcgttac catcgaagac
     4441 ccgattgagt ttatctacaa accgcgccgc tgcatcttta cccagcgcga aatcggcgtc
     4501 gacaccataa actggcagac ggcggtacaa aacgctatgc gccaatcccc cgacgtggtc
     4561 tgcatcggcg aagtccgcag cagggaaagt atggaatacg cgatgcagct cgcccaaacc
     4621 ggccacctgt gcatttttac gctccacgcc aacaccgcgc cgcagtcgct cgaacgcata
     4681 ctcaacttct accccaaaga acagcacaac caaatactga tcgacatcgc cctcaacctg
     4741 accggcatca tctgccaacg cctcgccctc aaacaagaca aaacgggcag gacggcggtt
     4801 gtcgacttgc tcatcaacac gcccgccatc caagacttca tcctgaaggg cgacctgatg
     4861 aacatcagta aaatcatgga aaccgccaaa accgacggaa tgcagacgat ggatcaaaac
     4921 cttttcgaac tgtaccgtca cggcatcatc agttacgaag aagccctgcg ccagtccgtt
     4981 tccgccaaca acctgcgatt gcacatccaa ctgcacaaag aaggcaaaac gcccgaactc
     5041 ctttacgaca gggtcaacgg tctcaacctc atttcctgat ccgcaaaacc caatgccgtc
     5101 tgaaaaccgc atccccgttt tcagacggca tgattttatc cgccccattc atgtgctaca
     5161 ctttatagtg gattaaattt atagtggatt aacaaaaatc aggacaaggc gacgaagccg
     5221 cagacagcac aaatagtacg gaaccgattc acttggtgct tcagcacctt agagaatcgt
     5281 tctctttcag ctaaggcgag gcaacgccgt actggttttt gttaatccac tatattcact
     5341 tcataacaat aaaccggtaa aaccatgaaa accccactcc tcaagcctct gctcattacc
     5401 tcgcttcccg ttttcgccag tgtttttacc gccgcctcca tcgtctggca gctaggcgaa
     5461 cccaagctcg ccatgccctt cgtactcggc atcatcgccg gcggccttgt cgatttggac
     5521 aaccgcctga ccggacggct gaaaaacatc atcgccaccg tcgccctgtt taccctctcc
     5581 tcgctcacgg cacaaagcac cctcggcaca gggctgccct tcatcctcgc catgaccctg
     5641 atgaccttcg gctttaccat tttaggcgcg gtcgggctga aataccgcac cttcgccttc
     5701 ggcgcactcg ccgtcgccac ctacaccaca cttacctaca cccccgaaac ctactggctg
     5761 accaacccct tcatgatttt atgcggcacc gtactgtaca gcaccgccat catcctgttc
     5821 caaatcatcc tgccccaccg ccccgtccaa gaaagcgtcg ccaacgccta cgacgcactc
     5881 ggcggctacc tcgaagctaa agccgacttt ttcgatcccg acgaagccga atggataggc
     5941 aaccgccaca tcgacctcgc catgagcaac accggcgtca tcaccgcctt caaccaatgc
     6001 cgttccgccc tgttttaccg ccttcgcggc aaacaccgcc acccgcgcac cgccaaaatg
     6061 ctgcgttact actttgccgc ccaagacata cacgaacgca tcagctccgc ccacgtcgat
     6121 tatcaggaaa tgtccgaaaa attcaaaaac accgacatca tcttccgcat ccaccgcctg
     6181 ctcgaaatgc agggacaagc ctgccgcaac accgcccaag ccctgcgcgc aagcaaagac
     6241 tacgtttaca gcaaacgcct cggccgcgcc atcgaaggct gccgccaatc gctgcgcctc
     6301 ctttcagaca gcaacgacag tcccgacatc cgccacctgc gccgccttct cgacaacctc
     6361 ggcagcgtcg accagcagtt ccgccaactc cagcacaacg gcctgcaggc agaaaacgac
     6421 cgcatgggcg acacccgcat cgccgccctc gaaaccggca gcctcaaaaa cacctggcag
     6481 gcaatccgtc ctcagctaaa cctcgaatca ggcgtattcc gccatgccgt ccgcctgtcc
     6541 ctcgtcgttg ccgccgcctg caccatcgtc gaagccctca acctcaacct cggctactgg
     6601 atactactga ccgccctttt cgtctgccaa cccaactaca ccgccaccaa aagccgcgtc
     6661 cgccagcgca tcgccggcac cgtactcggc gtaatcgtcg gctcgctcgt cccctacttt
     6721 accccctccg tcgaaaccaa gctttggatc gtcatcgcca gtaccaccct ctttttcatg
     6781 acccgcacct acaaatacag cttctcgaca tttttcatca ccattcaagc cctgaccagc
     6841 ctctccctcg cagggttgga cgtatacgcc gccatgcccg tacgcatcat cgacaccatt
     6901 atcggcgcat cccttgcctg ggcggcagtc agctacctat ggccagactg gaaatacctc
     6961 acgctcgaac gcaccgccgc ccttgccgta tgcagcaacg gcgcctatct cgaaaaaatc
     7021 gccgaacgcc tcaaaagcgg cgaaaccggc gacgacgtcg aataccgcgc cacccgccgc
     7081 cgcgcccacg aacacaccgc cgccctcagc agcacccttt ccgacatgag cagcgaaccc
     7141 gcaaaattcg ccgacagcct gcaacccggc tttaccctgc tcaaaaccgg ctacgccctg
     7201 accggctaca tctccgccct cggcgcatac cgcagcgaaa tgcacgaaga atgcagcccc
     7261 gactttaccg cacagttcca cctcgccgcc gaacacaccg cccacatctt ccaacacctg
     7321 cccgaaaccg aacccgacga ctttcagaca gcactgaata cactgcgcgg cgaactcgac
     7381 accctccgca cccacagcag cggaacacaa agccacatcc tcctccaaca gctccaactc
     7441 atcgcccggc agctcgaacc ctactaccgc gcctaccgac aaattccgca caggcagccc
     7501 caaaacgcag cctgaaaaag tttcggcatt ttgtaagaga gagcagattg tcagacaggt
     7561 tgcaagatag tggataaagc tttgcgcagg gtaaatgcgt agcaactgaa ccgtcattcc
     7621 cacgaatcta cattccgtca ttcccacgaa aacagaaaac
//
LOCUS       DAWHRA010000096         7605 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWHRA010000096 DAWHRA010000000
VERSION     DAWHRA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.096"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            103..327
                     /locus_tag="WIB30_001541"
     CDS             103..327
                     /locus_tag="WIB30_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ3797588.1"
                     /translation="MKVISVLNQKGGSGKTTVATHLARALQLAGNSILLVDSDPQGSS
                     RDWAAVHEEQPVPVIGIDSPTIDRDLKSVV"
     gene            340..747
                     /locus_tag="WIB30_001542"
     CDS             340..747
                     /locus_tag="WIB30_001542"
                     /inference="COORDINATES: protein motif:HMM:NF025001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEZ3797589.1"
                     /translation="MIDGAPQTADLAISAIKASDLVLIPVQPSPYDIWATADLVNLIK
                     QRIKLIDGNLKAAFLINRTIKGTKIGGEIRAALEEYELPVLSTVIVQRIIYPTSAITG
                     STVLDKEPNGEAAKEIQSLANEIINLINIDNHI"
     gene            790..993
                     /locus_tag="WIB30_001543"
     CDS             790..993
                     /locus_tag="WIB30_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEZ3797590.1"
                     /translation="MNGLKADRPSANKANLSIDDVIKQEEIKRISFDMSESLHRELKK
                     YAAENGVTIRVVLNKLVSDLLNK"
     gene            1169..2332
                     /locus_tag="WIB30_001544"
     CDS             1169..2332
                     /locus_tag="WIB30_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ3797591.1"
                     /translation="MHINFSMEYKEFNENYLYKKPFIFKNALDVSSISWKEINELYQR
                     ADPTDWQFKFRKGEIIPKEAYVESFNDVGRIRHRFNKTAVYQYLQDGATMVYNRIDNE
                     PFVDSIAKQIAQFAQAQTVVSGYLAFGSSPSYRNHWDTRDVFAVQLIGKKHWTVSAPN
                     FDMPLYMQQAKDMPHITPPTTVDMEVILEAGDILYIPRGWWHNPMPMNCETFHLAIGT
                     FPPNGYNYMEWLMKKIPDIQSIRQNFIDWEHDQKNIDNAAQAVTEMMKNQENYQAFIQ
                     DFLGNQRVNTAFNMQIFGNLDNDRLPENSTIKLNSLDNRTIKQGYIIANGIKTNLDND
                     SQTILQWIADKHSVKLTQLYEFCQNQNINLEKVEKLVFDLTMIDVLECLTDER"
     gene            2339..2545
                     /locus_tag="WIB30_001545"
     CDS             2339..2545
                     /locus_tag="WIB30_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797592.1"
                     /translation="MFTFLNKNIFIKTIVLGCFYSFLFFSFFVSGKSFGYSFEIFNMD
                     TSALAFLSVFMGFFISVAVHIYDI"
     gene            2569..2904
                     /locus_tag="WIB30_001546"
     CDS             2569..2904
                     /locus_tag="WIB30_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ3797593.1"
                     /translation="MKELTINDLTLVSGGFEFGRDYIEIAPVVGTAGGVASSVRAASM
                     TGEAGAILTRSGVTGLRAGVVAIPLLAYNGTVWVNRNTKLQDNLGRLIDKVTGLDQSG
                     NDYGDGTSY"
     gene            2939..5035
                     /locus_tag="WIB30_001547"
     CDS             2939..5035
                     /locus_tag="WIB30_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ3797594.1"
                     /translation="MSIYLSRLSFGFSRRLLVVLQTEAAECGLACLVSVLGFHGFYTD
                     LRHLRARFSLSLKGATLADLVRFANSMNLTARAVRLDLDELVNLRLPCILHWDLNHFV
                     VLHEVHRNHIVIMNPASGWQKVRMEEVSRCFTGVALELFPNTQFEEKHETRKIRVLPM
                     LRGVVGLKRSLFQLLLLAAVLQVFALASPFFMQWVIDHAIVSADRDLLMTLALCFGLL
                     MIVRQLVSLLQTWAGMYLSTSLNIQWKANVLRRLMDLPVSYFAKRHLGDVVSRFGSVD
                     SIQSTLTSTFFVLVLNSIMAVFTLGLMYLYSPSLTTVVLVVLLIYIGIRWVAYYPLRH
                     ATQENIVHAAKQSSYFMETIRGIQTVKLFDKNAQRHAAWLNLFIDTINTGLMTQKLSA
                     LFGFTNGLLFGIANILIVYFGAVSVLNGGFTVGALMAFMSYKSQFESKAGSLIDQFVQ
                     IKMLGLHAERLADIVLEETENEQNNDFSLPKNLEHFDIQIENVSFSYAENEPYILQNF
                     SLIIKQGTAIAFAGHSGCGKSTLIQILTGSLKPESGHVLLGGHDIHALPPAFIRTWSA
                     SVMQNDVLFTGSIAENISFFDDTPNMEKIAFCAQMANIHHEIVAMPMAYETLIGDMGS
                     ALSGGQKQRIVLARALYREPKILFLDEASSHLDINNERVINDNLRQLNITKIMVAHRQ
                     ETLNTADSVVYLDKVA"
     gene            5037..6299
                     /locus_tag="WIB30_001548"
     CDS             5037..6299
                     /locus_tag="WIB30_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ3797595.1"
                     /translation="MTLFRPEVFQAKKNRWTGQIVLVRPFSLQFLTFFAVSLAAILVA
                     FLIFGSYTNKTTVTGQLLPTTGVVRVYSQDMGVIAHQHVMNGDFVKKGDVLFTLSTSR
                     NDNNGSIQARLLAEAELKKSLSEQEIIMKKRVHAAEKTAQENTVHRFQNQMQHVRNQI
                     IMQEKRIAISEKMLEKQRYLAKMDAISELEKNSYEIALLELKAGLAAYQREADNLARE
                     ITVQQSNLKNLPEQQATEISQLERAVSVYQQEILDYQQRNEQTIRATISGYVSSINTE
                     IGQQVDTNKLLMSIVPKESELLANLYVPSRAIGFVKPNDKVILRYQAYPYQKFGHAEG
                     HVISIAQTALGAQEWTNLGNIFTQTAQVNEPVYLIKVKLDNQHIRIYGTEKKLQIGMI
                     LEADILHENKRLYEWILDPLYQVMGKIS"
     gene            complement(6569..7408)
                     /locus_tag="WIB30_001549"
     CDS             complement(6569..7408)
                     /locus_tag="WIB30_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797596.1"
                     /translation="MGNFAKVSNCEFWDKINYNRAAQAAEQRAKQAQETPKEPPKQEE
                     HFIAKLPNDKLMTIYRLFQKQLDDMRGLERDIERGDWYINARYRMNENRNKIRALEDD
                     NKRLHHDYWAVQDQGENRFFGMGKIGAKLVKENEKQQHINERHGANVREIRRLKTENE
                     KLEGQYSDKLAADVVKVRDRFNEELDNVRREIGKRPMEEFRNVFLQAAQEAQTTIGQK
                     AVQIELEKFPLNGSPSAKRIAFYFGAQRIREVEQKEREDQRKEQERQARRMERDDGHG
                     IGM"
     gene            7449..>7605
                     /locus_tag="WIB30_001550"
     CDS             7449..>7605
                     /locus_tag="WIB30_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ3797597.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGR"
BASE COUNT         2300 a         1375 c         1545 g         2385 t
ORIGIN      
        1 aatttaaaac aaaatgtaaa tattctttta tttatttaaa taatagatta aaatattatt
       61 taattaaaag cataatgagc gtagatattc aaaggagcta acatgaaagt tatttccgta
      121 ttaaatcaaa agggtggaag tggtaaaacc acagtcgcaa ctcacttggc acgcgccttg
      181 caattagcag gaaatagcat cttattggtt gatagcgatc cacaaggaag cagtagggac
      241 tgggctgctg ttcacgaaga gcaacctgtt cctgtgattg gtattgacag cccgacaatt
      301 gatcgtgatt taaaaagcgt tgtttaaaaa gattttgtta ttattgatgg cgcaccacaa
      361 acagccgatt tagccatatc agcgattaaa gcctctgatt tagttttaat ccctgttcaa
      421 ccgtcgccat acgatatttg ggcaaccgct gatttggtta atttaatcaa gcagcggata
      481 aaactcatag atggaaattt aaaggctgct tttttaatta accgaactat taaaggaaca
      541 aagattggcg gagaaattag agcagcgtta gaagaatatg agttacctgt tttatcaacc
      601 gtgattgtgc aacgtattat ttatccaaca tctgctatca caggtagcac tgtactggat
      661 aaagaaccaa atggagaagc agctaaggaa attcaatcac ttgcgaatga gataataaat
      721 ttgataaata tagataatca tatttaatga aatattattt taaattttta aatattgtag
      781 gaaataatta tgaacggatt aaaagcagat cgtcctagtg caaacaaagc aaacttatct
      841 attgatgatg tgatcaagca agaagaaatc aagagaatca gttttgatat gtctgaatct
      901 ctgcatagag aattgaagaa atacgcggct gagaatggtg taactattcg cgtcgtgctc
      961 aataaactcg tttcagattt attgaataaa taataacaca cggcagaatg tatttgacca
     1021 taatgtcaaa gcaatatcca actttatcta aaaataacaa aataccttct tccaaaaaca
     1081 gaacgtcttt tcattgcaaa acttgacata taaacactag ttaagtaaac ttaattttca
     1141 ttattaataa tgaaaataag gattattttt gcatattaac ttttcaatgg aatataaaga
     1201 atttaatgaa aattatttat ataaaaagcc atttattttt aaaaatgctt tagatgttag
     1261 ttctatctca tggaaagaaa ttaatgagtt gtatcaacgt gcagacccta ctgattggca
     1321 atttaaattt cgcaaagggg aaataatccc caaagaagcc tatgttgaat cattcaatga
     1381 tgtgggtaga atacgtcatc ggtttaataa aacagccgta tatcagtatc tgcaagatgg
     1441 tgcaacaatg gtttataacc gtattgataa tgagccattt gttgacagta ttgctaaaca
     1501 aattgcccag tttgctcaag cacaaactgt tgttagtggt tatttggcgt ttggtagttc
     1561 tccatcatat cgtaatcatt gggatactcg tgatgtgttt gcggttcaat tgattggcaa
     1621 aaaacactgg acagtttctg ctccaaattt tgatatgcca ttgtatatgc agcaagcaaa
     1681 agatatgccg cacattactc cgcctacaac agtggatatg gaagtcattt tagaagcagg
     1741 agatatttta tatatcccgc gtggttggtg gcataatcct atgcctatga attgtgaaac
     1801 attccattta gctattggaa cttttccgcc aaatggttat aattatatgg aatggttgat
     1861 gaaaaaaata cctgatatcc agagtattcg tcaaaatttc atagactggg aacacgacca
     1921 aaaaaatata gataatgcgg ctcaggcagt tactgaaatg atgaagaacc aagaaaatta
     1981 tcaagcattc atacaagatt ttttgggaaa tcaacgcgta aatactgcat ttaatatgca
     2041 aatttttgga aatctggata atgacagatt acctgaaaat agtacaatta aactcaattc
     2101 gttggataac cgtacaatta aacaaggata tatcatagcg aatggcatca aaaccaactt
     2161 ggacaatgat agccaaacga ttttgcaatg gattgctgac aaacatagtg ttaagttaac
     2221 tcaactgtat gaattttgcc aaaatcaaaa cattaatttg gaaaaagtgg aaaaattagt
     2281 gtttgatttg acaatgatcg atgtattgga atgtttaact gatgaaagat aaattattat
     2341 gtttactttt ttaaataaaa atatatttat caaaacaatt gtacttggct gtttttattc
     2401 ctttttattt tttagttttt ttgtatctgg taagtcattt ggttattcat ttgaaatttt
     2461 taatatggat acaagtgcgc ttgctttttt aagtgtattt atgggctttt ttataagcgt
     2521 ggctgtgcat atttacgata tttaatacaa ttgaaaagat aaataattat gaaagaatta
     2581 actattaatg atttaaccct agtttcgggt ggatttgaat ttggtcgtga ttatattgaa
     2641 atagctccag ttgtaggtac agcaggtggc gttgcctctt ctgtgagagc agcttcaatg
     2701 actggagaag ctggagcaat tcttactcgt tcaggagtaa caggattacg tgctggagtg
     2761 gtagctattc cattgttagc ctacaatggt actgtatggg ttaatagaaa taccaaatta
     2821 caagataatt taggtagatt aatagataaa gtaactggcc tggatcaatc aggcaatgat
     2881 tatggcgatg ggacaagtta ttaatctcat aatactttaa aatcaaaaaa gaacaccaat
     2941 gtcaatatat ctatctcgcc tatcattcgg cttttctcgc cgtttactag ttgtcttgca
     3001 aacggaagca gcagaatgtg gattggcgtg tttggtatcg gtattggggt ttcatggttt
     3061 ttatacggat ttgcgccatt taagggcgcg tttttctttg tctcttaaag gcgcaacatt
     3121 ggcagatttg gtacggtttg ctaatagcat gaatttaact gctcgagctg ttcggttgga
     3181 tttagatgaa ttggtaaatt tgcgcttacc ctgtatttta cattgggatt taaatcattt
     3241 tgtggtgttg catgaagttc atcgtaatca cattgtgatt atgaaccctg cttcgggttg
     3301 gcaaaaagtg cgtatggaag aagtctcgcg ctgttttaca ggtgtagcgt tggaactatt
     3361 tcctaacacg caatttgaag agaaacacga aacgcgtaaa attcgcgtac taccgatgtt
     3421 gcgcggagtg gtgggattaa aaaggtcttt atttcagctt ctgctgctgg cggcagtatt
     3481 acaggtgttt gctttggcaa gccctttttt tatgcaatgg gtaatagacc atgccattgt
     3541 atcggcagac cgtgatttat tgatgacatt ggcattgtgt tttggcttgt tgatgattgt
     3601 gcggcagtta gtgtcattgt tgcaaacttg ggcgggtatg tacttatcta cctctttgaa
     3661 tattcaatgg aaagctaatg tattacgccg cttaatggat ttgcctgttt cttatttcgc
     3721 taaacgacat ttgggcgatg tggtttcacg ttttggctca gtggacagta ttcaaagtac
     3781 gctcacttct actttctttg tgttggtgct caacagcatt atggcggtat ttacattagg
     3841 gttgatgtat ttgtatagcc caagtttaac gacagttgta ctggttgttt tgctgattta
     3901 tattggtatt cgttgggtgg catattatcc attacgccat gcaactcaag aaaatattgt
     3961 tcatgctgcc aagcagagca gttatttcat ggaaaccata cgtggtattc aaactgttaa
     4021 attatttgat aaaaatgcac aacgccatgc ggcttggctg aacttgttta ttgacacgat
     4081 taacacaggt ttaatgacac aaaaactttc cgcgctgttc ggttttacga atggcctttt
     4141 gtttggtatc gctaatattt taattgtgta ttttggtgcg gtttctgtat tgaacggtgg
     4201 ttttaccgtt ggggcgttaa tggcgtttat gtcctacaaa agtcagtttg agagtaaagc
     4261 aggttcattg attgaccagt ttgtacaaat caaaatgcta ggattacacg cggaacgctt
     4321 ggcggatatt gtgctagaag aaacggaaaa tgaacaaaac aacgatttca gtctgcctaa
     4381 aaatttagaa cattttgata ttcaaataga aaatgtatca tttagctatg ctgaaaatga
     4441 accatacatt ttgcaaaatt tcagtttaat catcaaacaa ggaacagcca ttgcgtttgc
     4501 tggacattca ggctgtggga aatctacgct cattcaaatt ctgacaggca gtctgaaacc
     4561 tgaaagcggt catgtgttat taggtgggca tgacattcat gcgttaccac ctgcatttat
     4621 ccgcacatgg agcgcaagtg tgatgcaaaa tgatgtgtta tttactggct caatcgcaga
     4681 aaacatcagt ttttttgatg atacgcccaa tatggaaaaa atcgcatttt gcgctcaaat
     4741 ggcgaatatt catcatgaaa ttgtcgctat gcctatggcg tatgaaacat tgattggcga
     4801 catggggagt gcattgagtg gtgggcaaaa gcagcgcatc gtactagctc gtgctttata
     4861 tcgtgaacca aaaattttgt ttttagatga agccagtagc catttggaca tcaacaatga
     4921 acgtgtgatt aacgataatc tacgccaact caacattacc aaaatcatgg tcgcgcatcg
     4981 tcaggaaact ttaaatactg ctgatagcgt agtttattta gacaaggtag cctgaaatga
     5041 cattatttcg ccccgaagta ttccaagcta aaaaaaatcg ctggacaggg caaattgttt
     5101 tagtgcgtcc tttttccttg cagtttttaa catttttcgc ggtctcatta gcggctattt
     5161 tagttgcttt tttaattttt gggagctata ccaataaaac caccgtaaca ggacaacttt
     5221 tgccgacaac aggggtagta cgcgtatatt cgcaggatat gggggttatt gctcatcaac
     5281 acgttatgaa tggcgatttt gtgaaaaaag gcgatgtgtt atttacatta tctacttcac
     5341 gaaatgacaa taatggcagc atacaagcac gattactcgc cgaagctgaa ttaaaaaaat
     5401 cactatcaga acaagaaatt attatgaaaa aacgtgttca tgcagcagaa aaaaccgcac
     5461 aagaaaacac ggtacaccgt tttcagaacc aaatgcaaca cgttagaaat caaattatca
     5521 tgcaagaaaa acgtattgcg atttctgaaa aaatgttaga aaaacagcgt tatttagcca
     5581 aaatggacgc aatttctgaa ttagaaaaaa atagttatga aattgcttta ttggagctga
     5641 aagcaggttt agccgcttac caacgagaag cagataatct tgctcgggaa ataactgtac
     5701 agcaaagcaa tctaaaaaat ctgcctgaac agcaagctac tgaaatcagt caattagaac
     5761 gcgcagtatc tgtctatcaa caagaaatat tggattatca acaacgtaac gaacaaacta
     5821 ttcgcgcaac catatcaggc tatgtcagtt ctattaacac ggaaattggt cagcaggtag
     5881 atactaacaa actattgatg agtatcgtgc cgaaagaaag tgaattactg gctaatttat
     5941 atgtccctag tcgcgcaatc ggttttgtaa aaccaaatga caaggtaatt ttgcgttatc
     6001 aagcatatcc ttatcaaaaa ttcggacacg cagaaggaca cgtcatttct attgctcaaa
     6061 cggcactagg ggcgcaagaa tggacaaatt taggcaatat ttttactcaa acagcacaag
     6121 tgaatgagcc tgtttattta atcaaagtaa aactggataa tcagcatatt cgtatatatg
     6181 gcacggagaa gaaattgcaa attggtatga tactagaagc cgatattttg catgagaata
     6241 aacggttata tgaatggata cttgaccctt tgtatcaagt aatgggaaaa atatcatagc
     6301 tgaattgctc aatacattgg agcgtatgag tgcccctaat tatcaagcaa aaccaatgtc
     6361 cgatgacttc caatttgttg ttaagcacaa atacacagtg ttttatgtaa taatgaagac
     6421 agagaagtga tacaattttt cgctccattt ttgcttcctg aattgctgca attgtatctg
     6481 cgttgtatgc gagaagtaaa tattttcgat gcatttgctg actttgtacc ataatacgtc
     6541 ttccaaccag tatgataggc tggtttttct acatgccgat gccgtggccg tcgtcccgct
     6601 ccatacgccg cgcctgccgc tcctgctctt tgcgctggtc ttcccgctcc ttctgctcta
     6661 cttcccttat ccgctgcgcg ccgaaataaa acgcgattcg cttggccgaa gggctgccgt
     6721 tcagggggaa tttttccagt tcgatttgaa ccgccttctg ccctattgtc gtctgtgctt
     6781 cttgggctgc ctgcaaaaat acgttcctga attcttccat cggccgtttg ccgatttccc
     6841 tgcggacatt gtccagttct tcgttaaacc tgtcgcgtac tttgaccacg tcggcggcca
     6901 acttgtcgct atactgccct tccagttttt cgttttctgt tttcagccgc cggatttctc
     6961 gcacgttcgc tccatgccgt tcgttgatgt gttgctgctt ctcgttttcc ttaaccagtt
     7021 ttgcgccgat tttacccatg ccgaaaaagc ggttttcgcc ttggtcttgg actgcccaat
     7081 agtcgtgatg cagccgtttg ttgtcgtctt ccagcgccct tattttgttt cggttttcgt
     7141 tcatgcggta tcgcgcattg atgtaccaat ctccgcgttc tatatcgcgc tccaagccgc
     7201 gcatgtcgtc cagttgtttt tgaaacagcc tgtatatcgt cattagcttg tcgttcggca
     7261 gcttggcgat aaagtgctct tcctgtttcg gcggctcttt cggtgtttcc tgcgcctgct
     7321 ttgcccgctg ctcggcggct tgggcagccc gattataatt aattttatcc cagaattcac
     7381 aatttgagac ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag acatttaggg
     7441 gatgttccat gagcaccttc ttccaacaaa ccgcccaagc catgattgcc aaacacatcg
     7501 accgtttccc gctattgaag ttggaccagg tgattgattg gcagccgatc gaacagtacc
     7561 tgaaccgtca aaaaacccgt taccttagag atcaccgcgg ccgtc
//
LOCUS       DAWHRA010000097         7552 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWHRA010000097 DAWHRA010000000
VERSION     DAWHRA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.097"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            144..725
                     /locus_tag="WIB30_001551"
     CDS             144..725
                     /locus_tag="WIB30_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ3797598.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(787..1641)
                     /gene="folD"
                     /locus_tag="WIB30_001552"
     CDS             complement(787..1641)
                     /gene="folD"
                     /locus_tag="WIB30_001552"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ3797599.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1864..1941
                     /locus_tag="WIB30_001553"
     tRNA            1864..1941
                     /locus_tag="WIB30_001553"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1899..1901,aa:Pro,seq:tgg)
     gene            1966..2042
                     /locus_tag="WIB30_001554"
     tRNA            1966..2042
                     /locus_tag="WIB30_001554"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2000..2002,aa:Arg,seq:tct)
     gene            2070..2145
                     /locus_tag="WIB30_001555"
     tRNA            2070..2145
                     /locus_tag="WIB30_001555"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2103..2105,aa:His,seq:gtg)
     gene            2357..2863
                     /locus_tag="WIB30_001556"
     CDS             2357..2863
                     /locus_tag="WIB30_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ3797600.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2860..4638
                     /locus_tag="WIB30_001557"
     CDS             2860..4638
                     /locus_tag="WIB30_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ3797601.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4751..5575
                     /locus_tag="WIB30_001558"
     CDS             4751..5575
                     /locus_tag="WIB30_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ3797602.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(6338..7126)
                     /locus_tag="WIB30_001559"
     CDS             complement(6338..7126)
                     /locus_tag="WIB30_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ3797603.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPYTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(7340..7534)
                     /gene="thiS"
                     /locus_tag="WIB30_001560"
     CDS             complement(7340..7534)
                     /gene="thiS"
                     /locus_tag="WIB30_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ3797604.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         1610 a         1949 c         2259 g         1734 t
ORIGIN      
        1 aattatgtat ggcgtaaagg aagcgtttta acgccgtatt gggaaaatgt aaagttcttt
       61 atcgggattt ctttaagtat tggcgttttc ggtgtcgtgg cggtagaatc ggggtcagtt
      121 aagaacaatt atcaggaacg gatatggaag aaaaaaacga ctacaccgac gcggcatccg
      181 acaaccgcaa cgggcaggaa attgaagtcg gaattgccgg cgcaggcgac cggatacttg
      241 ccgcgctgct gaaccaattg tttacctttt tggttttgtt ggtgccgttt gccggactga
      301 ttgctttcgc catcaagaat gaaggcagga ttggcggcag tgaggaaata ttcgggctgc
      361 ttttgggcat gacttccttt tgggtaggac tggcgggtat tttggtttat accgtcgttc
      421 aaatctacta tatgagtcgg gacgggcagt cattgggtaa gaaaatcatg agaatcaggg
      481 tgttgaaaac cgacggccgc aatcccggtt ttgtcggcac ggttttggta cgcgaaatcg
      541 catggtcggt tttggttgcc attattgccg ccgttatcgg tcttgcagta ggtgacaacg
      601 gagaaaacgc catcaacctg ctggcattcc ttgccaactt tgtcctgctc tttatggtca
      661 aacgcgaccg ccgcacgctt tacgacatac tggcggatac ggttgtcgtc aagctgccca
      721 aataaacgga cacggcaaaa tgccgtctga aagcctttca gacggcattt ttatcttcag
      781 aaccgcttaa gcatcgtgca gcgaagccgc gtgcaaagtg ttttccatca atgtggcaat
      841 cgtcatcgga cccacgccgc cgggaacggg cgtaatcatc gccgcccgtt cttttgccgt
      901 ttcaaattcc acgtcgccgc acaggctgcc atcgtccaaa cggttgatgc ccacatcaat
      961 aacgaccgcg ccaggtttga tccattcgcc tttgacaaag ttcggaatgc ctacgccgac
     1021 caccaaaata tcggctccgg caacctcgtc tgtcagattt tcggttgcgc tgtggcagac
     1081 cgttaccgtt gcgcgcgcca gcagcagttc caaagcctgc gggcggccga cgatattcga
     1141 cgcgccgacc acgaccgctt ttttcccctt cggatcaatg ccgtaagctt ccaaaagcgt
     1201 catcacgccc ttgggcgtac acgggcgcat cagcggcatt ttgaccgcca gcctgccgac
     1261 attgtaagga tggaagccgt ccacgtcctt atccggcgaa atacgttcca aaaccgcctg
     1321 gctgtcgagg tgcttcggca gcggtagctg aaccagaata ccgtccactt cggaatcggc
     1381 attcaggcgg tcgaccagtg ccagcagttc ttcctgcgat gttgattcgg gcagctcgta
     1441 agacagtgat ttgatgccgc atttttggca ggcagttttc ttgttgcgga cataaaccgc
     1501 gctggcaggg tcgcctccga ccaaaaccac ggccaggcaa ggcatgtgca gattgtcctg
     1561 ttggcgttgc gccaccgctt cggcaaccgc ctgcaggcgt ttttgcgaaa cttctttacc
     1621 attgatcagt tgtgccgaca tatccgtcct tttgttaaac aggtagaaaa tgctgaaaat
     1681 tatcgcattt tccgtttgtt tttacagcag gatgccgaaa ataaatttgc cctgaaataa
     1741 aatacggctg tttggacgga ttaaatattt tttcaaatca aaaaactata tttttcatct
     1801 tggtgtcttg aaaataaatg ttgacgcatc tttccctgag ttgtatagtt cgctacttca
     1861 agtcggggcg tagcgcagcc cggttagcgc atctgctttg ggagcagagg gtcgtgagtt
     1921 cgaatcccac cgccccgacc aatcaatcag cacgaatcgg catgagcgcc cgtagctcaa
     1981 ccggatagag caccgacctt ctaagtcggg ggttacaggt tcgattcctg tcgggcgtgc
     2041 caagaatttg attgagaacc gtttttatgg tggctgtagc tcagttggta gagccccgga
     2101 ttgtgattcc ggttgtcgtg ggttcgagcc ccatcagcca ccccagataa aggcccgtac
     2161 ttttcggtac gggtcttttt ccgtttgcac gtactgtccg caccgtcgta tcggtgcggc
     2221 ggtatgccat ctgaaagctg ggccggtcat acggtaaccg cttcagacgg catttgcgcc
     2281 ttcggctttg cctttcgcat atgcttctgt atcatgtggg tttttctgtc ttttatggtt
     2341 tttgtcggag actttgatgg ttgacgcttg gtctgtccct gattttgaat cgaaaatctg
     2401 tccgcccgag gcgttggcgg cgcgtttggc gttgttgccg cgcccgctgg tgtttaccaa
     2461 cggctgtttc gacatcctcc acagggggca cgttacttat ctggcgcagg cgcgttcgat
     2521 ggggggcgcg ttggtgctgg cgttgaatac cgatgcttcg gtgcggcgtt tgggcaaggg
     2581 cggcgaccgc ccgattaatc ctttggagaa ccgtgccgcc gttgccgccg ctttggaaag
     2641 tgtggatttg gtaacgtggt ttgacgggga tacgccggcg gcgttgattg aggcggtcaa
     2701 acctgagatt ttggtcaagg gcggcgattg ggctgcggat aagattgtcg gtgcggcaga
     2761 aacgttggcg cgcggcggtc aggtgttttc aattccgttt ctgcaccaga cttcgacaac
     2821 gaagactttg gcaaaaatcc gtgcggcaga gggcggaaaa tgacggtttt gaagccttcg
     2881 cactggcggg tgttggcgga gcttgccgac ggtttgccgc aacacgtctc gcaactggcg
     2941 cgtatggcgg atatgaagcc gcagcagctc aacggttttt ggcagcagat gccggcgcac
     3001 atacgcgggc tgttgcgcca acacgacggc tattggcggc tggtgcgccc attggcggtt
     3061 ttcgatgccg aaggtttgcg cgagctgggg gaaaggtcgg gttttcagac ggcattgaag
     3121 cacgagtgcg cgtccagcaa cgacgagata ctggaattgg cgcggattgc gccggacaag
     3181 gcgcacaaaa ccatatgtgt gacccacctg caaagtaagg gcagggggcg gcaggggcgg
     3241 aagtggtcgc accgtttggg cgagtgtctg atgttcagtt ttggctgggt gtttgaccgg
     3301 ccgcagtatg agttgggttc gctgtcgcct gttgcggcag tggcgtgccg gcgcgccttg
     3361 tcgcgtttag gtttggatgt gcagattaag tggcccaatg atttggttgt cggacgcgac
     3421 aaattgggcg gcattctgat tgaaacggtc aggacgggcg gcaaaacggt tgccgtggtc
     3481 ggtatcggca tcaattttgt cctgcccaag gaagtagaaa atgccgcttc cgtgcaatcg
     3541 ctgtttcaga cggcatcgcg gcggggcaat gccgatgccg ccgtgctgct ggaaacgctg
     3601 ttggtggaac tggacgcggt gttgttgcaa tatgcgcggg acggatttgc gccttttgtg
     3661 gcggaatatc aggctgccaa ccgcgaccac ggcaaggcgg tattgctgtt gcgcgacggc
     3721 gaaaccgtgt tcgaaggcac ggttaaaggc gtggacggac aaggcgtttt gcacttggaa
     3781 acggcagagg gcaaacagac ggtcgtcagc ggcgaaatca gcctgcggtc cgacgacagg
     3841 ccggtttccg tgccgaagcg gcgggattcg gaacgttttc tgctgttgga cggcggcaac
     3901 agccggctca agtgggcgtg ggtggaaaac ggcacgttcg caaccgtcgg tagcgcgccg
     3961 taccgcgatt tgtcgccttt gggcgcggag tgggcggaaa aggcggatgg aaatgtccgc
     4021 atcgtcggtt gcgccgtgtg cggagaattc aaaaaggcac aagtgcagga acagctcgcc
     4081 cgaaaaatcg agtggctgcc gtcttccgca caggctttgg gcatacgcaa ccactaccgc
     4141 caccccgaag aacacggttc cgaccgctgg ttcaacgcct tgggcagccg ccgcttcagc
     4201 cgcaacgcct gcgtcgtcgt cagttgcggc acggcggtaa cggttgacgc gctcaccgat
     4261 gacggacatt atctaggggg aaccatcatg cccggtttcc acctgatgaa agaatcgctc
     4321 gccgtccgaa ccgccaacct caaccggcac gccggtaagc gttatccttt cccgaccaca
     4381 acgggcaatg ccgtcgccag cggcatgatg gatgcggttt gcggctcggt tatgatgatg
     4441 cacgggcgtt tgaaagaaaa aaccggggcg ggcaagcctg tcgatgtcat cattaccggc
     4501 ggcggcgcgg caaaagttgc cgaagccctg ccgcctgcat ttttggcgga aaataccgtg
     4561 cgcgtggcgg acaacctcgt cattcacggg ctgctgaacc tgattgccgc cgaaggcggg
     4621 gaatcggaac atacttaaaa aatacaccct ttttcaacac ggggcggcat ccgtccgaat
     4681 ccgtccggat tctgccgccc gacagactgc cgcccaggcg ggcggggttt caaacattga
     4741 ggaaattcta atgaaatggc tcttcaccct tttggcggta ctcaatattg ccgtattcgg
     4801 cggcacggtg ggctataaat tggccgttaa agcgacgggc ggcgtgccgg aaaaccgggc
     4861 cgtcgaaaac acgccccccg caacgcccgc cgccgacaat gcggcggcat ccgtggagga
     4921 tacggcggca ctgctcaaac cgggcgacat cctgaccgaa gagcaggccg agcagttgcg
     4981 cttgaaaaag gaagcggaac agaaaaaact gagggagaaa aaacagcgtg aagaaaaagc
     5041 ccgccgcgaa aaactcgccg cagaaaaggc gcaggcggaa cgcgaaaacg gcgcggcgga
     5101 tgccttatgc gccgcgcagg caagcctcac gatggacgaa gatgactacc accgcatcaa
     5161 aggacttttg ggcaaatggt cgcacgttgc cagcaggagc gtcgaaaaac gcaccgccaa
     5221 agccaaacct gccgacaaaa cctaccgcgt cgtcctgccc gtttccgccg atgccgaaaa
     5281 tcaggcggcg gagctgtctg ccaaaggttt caaccccata ccgtttgacg gcgcattgag
     5341 tttgggtgtc ggcaacagcc gggaaaacgc ccaagccctg caaaaccggc ttgccggcgc
     5401 cggattcggc ggggcgcata ttgtcgaaca ctttgccgaa gccgacaggc aggacgattc
     5461 tttgagcgtg tcgcgtatga cggttttgtt taccggcgtg aatgccgccg atgcggacga
     5521 aatccgtaaa atcacgtccc tatacggcaa actgaacctc aagtcttgca aataggcgcg
     5581 aaagccggac gataaacgcc aaacccaccg cgaaggtggg tttggcgttt cggttttgag
     5641 tatctgatat ataaaaaggg aatgtatgtc tcgtatatgc cgactccaag tgtgaaagtg
     5701 atgatgggga aatatggtgt ttaatggttc ggaacggacg gcttccgagt ggttttgatt
     5761 atacgctaat aataataatt atcaataaaa atttaaaaat aaatgcagat tttttgtaat
     5821 acgatgaaaa ataaacaaat taacctgtcc gccgatgttt gtgattcggg tcggcaaact
     5881 ttcattgtgt cggacaaatc ggggtcggca gtcatagctg agggcttcgt gcctgacgga
     5941 aataaggttg cccgtgtttg aacatccgtc cgattgggcg cggacagggt tgtcattcgg
     6001 gggtgtttga gcgtggattc ggggatgcga aagcggtaaa accgttgcgg atgaaggctt
     6061 acggacggct ttaggtattt ttaagtgtat tcaaacaaca acaaaacgct tcttctattg
     6121 ccgtatttag ggcttgcgcg gcagcctgca caccgtggag gccgagtgtt tcgttggttg
     6181 cggcatcggg acgggcggca aggttttccg gccggttttt ccattttttc cgacaaagcc
     6241 gtgatgccgg ggaatggcgg tttgtccgtt tcggaccgcc gccgttttga gccgcgatgc
     6301 cgtctgaagc cttcagacgg catttttgcc gcctttttca atattccgcc gaatgccaaa
     6361 acggctgtcc gactgtcggc gtgctggctt gcgctttgtc gcgtgcttcg accggtccgg
     6421 cttcaaatgc cagccgtccg gattcgacgg cgagtgcgaa ggcgcgtgcc atattgactg
     6481 gatcgccgct gcgggaaacg gcggtgttca aaagcacgcc gtcaaagccc cattccatca
     6541 cttgtgccgc ctgtgagggc aaacccaagc ccgcgtcgat aatcagcggc gtgtagggca
     6601 ggcgttcgcg caggacgttc aacgcgtagg cgtgaaccgc gcccaaaccc gtgccgatcg
     6661 gggccgccca cggcatcaac gcctgacagc ccacatcgag caggcggcgg caggcaatca
     6721 ggtcttcggt gcaataaggc agcactttga agccgtcttt aatcaggatt tccgccgctt
     6781 cgacaagttg gaacacatcc ggctgcaagg tgtcgtcgtc gccgatgagt tcgagtttaa
     6841 tccaatcggt ttcaaacact tcgcgcgcca tttgcgccgt cgttaccgct tcctgcacgc
     6901 tttggcagcc tgccgtgttc ggcaggacgg gaacgccggt ttcttgaagc agcgaccaaa
     6961 acccctgacc gtgtgcctcc ccaccgcttc ccgcgcggcg cagcgagacg gtaatcatcg
     7021 caggccgggc ggttcggacg gattgtttga ggatttcagg ggtcgggtag gcagccgtgc
     7081 cgagcagcag ccgcgagggg aaagtttctc cgtatagggt gagcataatg ggttcctttg
     7141 tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa gagaacgatt
     7201 ctctaaggtg ctgaagcact aagtgaatcg gttccgtact atccgtactg tctgcggctt
     7261 cgtcgccttg tcctgattta aagttaatcc accataacgt tgttttgggg acaggggtcg
     7321 tctgaaaagg caaaaccgcc tagccgccga ccaccggccg cacgatatcg attttgtcgt
     7381 tttcgttcaa aaccgtttcc gcatacgcgc ctttggggat gaaaacggtg ttgaccgcca
     7441 cggcaaaggg cttttgcggc gcggtttggg cgatgaggtc ggcaacgctt gtgccgtgaa
     7501 gttcggcggg tccgccgttt aagatgatgt tcatggtttc tcgatgtttc tg
//
LOCUS       DAWHRA010000098         7525 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWHRA010000098 DAWHRA010000000
VERSION     DAWHRA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.098"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..2136
                     /locus_tag="WIB30_001561"
     CDS             <1..2136
                     /locus_tag="WIB30_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ3797605.1"
                     /translation="RLATESAFIDKVFFVVDRKDLDYQTMKEYQRFSPDSVNGSESTT
                     GLKRNLEKDDNKIIVTTIQKLNNLMKSEDNLPVYHQRVVFIFDECHRSQFGEAQKNLK
                     KKFKKFCQFGFTGTPIFPENALGAETTAGVFGRELHSYVITDAIRDEKVLKFKVDYND
                     VRPQFKAVEAEQDEKKLSAAENRQALLHPERIREITQYILNQFRQKTHRLNAGGKGFN
                     AMFAVSSVDAAKCYYEAFKTQQAGSLHPLKVATIFSFAANEEQNAVGEIVDETFEPEA
                     MDSSAKEFLQAAINDYNACFKTNFGTDSKAFQNYYRDLAKRVKNQEVDLLIVVGMFLT
                     GFDAPTLNTLFVDKNLRYHGLMQAFSRTNRIYDATKTFGNIVCFRDLEQATIDAITLF
                     GDKNTKNVVLEKSYEEYMNGYTDSQTGEARRGYLDVAKELRQRFPDPDKIETEKDKKD
                     FAKLFGEYLRAENVLQNYDEFAALRELQSVDAADEDAMKAFQEKYYLSDEDVQEMRQV
                     PMPSERAVQDYRSAYNDIRDWLRRQKAGEQKEQSKIDWDDVVFEVDLLKSQEINLDYI
                     LQLVFEHHKKIKGKAELVEEIRRIIRASIGHRAKEGLIVDFINDTDLDKVPDVPAILE
                     TFYTYGQEVMRHEAAGLIAAEGLNETAAKRYLTGSLKRGYASENGTELTETLPKMSPL
                     NPQYLTKKQSVFQKIAAFVEKFAGIGTDI"
     gene            complement(2175..4466)
                     /gene="clpA"
                     /locus_tag="WIB30_001562"
     CDS             complement(2175..4466)
                     /gene="clpA"
                     /locus_tag="WIB30_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ3797606.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAINKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPVITKVVDKFLLQLEHRLLDKK
                     VEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(4468..4770)
                     /gene="clpS"
                     /locus_tag="WIB30_001563"
     CDS             complement(4468..4770)
                     /gene="clpS"
                     /locus_tag="WIB30_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ3797607.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            5013..5216
                     /locus_tag="WIB30_001564"
     CDS             5013..5216
                     /locus_tag="WIB30_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ3797608.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            complement(5513..6844)
                     /gene="pmbA"
                     /locus_tag="WIB30_001565"
     CDS             complement(5513..6844)
                     /gene="pmbA"
                     /locus_tag="WIB30_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ3797609.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVELAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVKGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            6991..7503
                     /locus_tag="WIB30_001566"
     CDS             6991..7503
                     /locus_tag="WIB30_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ3797610.1"
                     /translation="MKMFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLD
                     ADLYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLVRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
BASE COUNT         1731 a         1859 c         2179 g         1756 t
ORIGIN      
        1 cgtctggcga cagaatcggc gtttatcgac aaggttttct tcgtggtgga caggaaggat
       61 ttggactatc agacgatgaa ggaataccag cgtttttctc ccgacagcgt aaacggttcg
      121 gaaagcacga cgggcttgaa acgcaatttg gaaaaagacg acaacaaaat catcgttacc
      181 accatccaaa agctgaacaa cctgatgaag agtgaagata atctgccggt ttaccatcag
      241 cgggttgtct ttattttcga cgaatgccac cgctcgcaat tcggcgaagc gcaaaaaaac
      301 ctgaaaaaga aatttaaaaa attctgccag ttcggcttta ccggcacgcc gatttttccc
      361 gaaaacgctt tgggcgcgga aaccacggcg ggcgtgttcg ggcgggagct gcattcttat
      421 gtgattaccg atgccatccg cgatgaaaaa gtattgaaat tcaaagtgga ttacaacgat
      481 gtgcgcccgc agttcaaagc cgtggaagcg gaacaggacg agaagaaact gagtgccgcc
      541 gaaaatcgcc aagccctgct gcaccccgaa cgcatccgcg aaatcacgca atatatcctg
      601 aaccagttca ggcagaaaac gcaccggctg aacgcaggcg gcaaaggctt caacgccatg
      661 tttgccgtca gcagcgtgga tgcggcgaag tgctattatg aagcgttcaa aacacaacag
      721 gcaggcagcc tgcacccgct gaaagtggcc accatttttt cctttgcggc caacgaagag
      781 cagaacgccg tcggtgaaat tgttgacgag acctttgaac cggaagcgat ggacagcagc
      841 gcgaaagaat ttttgcaggc tgccatcaac gattacaacg cctgtttcaa aaccaatttc
      901 ggcacggaca gcaaagcctt tcagaactac taccgcgatt tggcaaaacg ggtgaaaaac
      961 caggaagtgg atttgctgat tgtggtcggc atgtttttga cgggttttga cgcgccgacg
     1021 ctgaatacgc tgttcgtcga taaaaacctg cgctatcacg gcctgatgca ggcgttttct
     1081 cgcaccaacc gcatttatga tgctaccaaa accttcggca atattgtctg cttccgcgat
     1141 ttggagcagg caaccattga tgcgatcacg ctcttcggcg ataaaaacac caaaaatgta
     1201 gtgctggaaa aaagctacga agaatatatg aacggctata ccgacagcca gaccggcgaa
     1261 gcgcggcgcg gttatctgga tgtggcaaaa gaattgcgcc aacgtttccc cgatcccgac
     1321 aaaatcgaaa cggaaaaaga caaaaaagat tttgccaaac tcttcggcga atacctgcgg
     1381 gcggaaaacg tattgcagaa ctacgatgaa tttgccgcgc tgcgtgaatt gcagagtgtg
     1441 gatgcggcgg acgaagatgc gatgaaggcg tttcaggaaa aatactacct gagcgatgaa
     1501 gacgtgcagg aaatgcggca agtgccgatg ccgtctgaaa gggcggtgca ggactaccgt
     1561 tccgcctaca acgacatccg cgactggctg cgccgccaaa aagcaggcga acagaaagag
     1621 caatcgaaaa tcgattggga cgatgtggtt tttgaggtgg atttgctcaa atctcaggaa
     1681 atcaatttgg attacatcct gcaactggtt ttcgaacacc acaaaaaaat caaaggcaaa
     1741 gcggagctgg tggaagaaat ccgccgcatc atccgcgcaa gtatcggaca ccgcgccaaa
     1801 gagggtctga ttgtggattt catcaacgat acggatttgg acaaagtacc cgacgttccc
     1861 gccatactgg aaacctttta cacctacggg caagaggtga tgcggcacga agcggcagga
     1921 ttgattgccg ccgaaggcct gaacgaaacc gccgccaaac gctatttgac cggctcgctc
     1981 aaacgcggct atgccagcga aaacggcacg gaactgaccg aaaccctgcc gaaaatgagt
     2041 ccgctcaacc cgcaatatct gacgaagaaa caaagtgttt ttcaaaagat tgcggcgttt
     2101 gtggagaagt ttgccggaat agggaccgat atttgacaaa atgccgtctg aaatttcaga
     2161 cggcattttt gattttatgc ggaggcggtt tttattttga ccttgctttt cttaaacttc
     2221 aacacggctt cttcttttgc cgcatcccag tctatccgta cgaagccgcc gtcggatagt
     2281 ttgccgaaca ggagttcgtc ggcgagcggt ttgcggattt tttcctgaat caggcggtgc
     2341 atcgggcgcg cgcccatttg cgggtcaaaa cctttttccg ccagatattt gtgcaatgcc
     2401 gacgtgaatt cggcttcgac ttttttgtcg aggagccggt gttcgagctg gagcaggaat
     2461 ttgtccacga ctttggtgat gacgggttcg gataagggcg caaacgggat aatcgcatcc
     2521 aagcggttgc ggaactcggg cgtgaagagc ttgttgatag cctgcatttc gtcgccgcgc
     2581 tcgcgtttgg cggtaaagcc gaggctgggt cggctgagac tctccgcacc tgcgttagtg
     2641 gtcataatta ggatgacgtt gcggaaatcg gcactcttgc cgttgttgtc ggtcagtttg
     2701 cctgcgtcca tgacttgcag gaggacgttg aaaatgtcgg ggtgggcttt ttcgatttcg
     2761 tccaagagca atacgcaatg cggctgtttg ttgatggctt cggtcaaaag gccgccttgt
     2821 tcaaagccga cgtagcccgg tggcgcgccg atgaggcgcg atacggcatg gcgttccatg
     2881 tattcggaca tatcaaagcg ttgcagcggt acgcccagcg agtaggcaag ctgtttggcg
     2941 gcttcggttt tgcccacgcc ggtcgggccg gagaagagga agctgcctat cggtttgtcg
     3001 ggcagggcaa ggccggaacg cgacattttg acggcggcaa ccagcgcgtc gatggcgttt
     3061 tcttgaccgt aaaccatgtt tttcaaatcg cgaccgagga attgcagcac ttgtttgtcg
     3121 tcgtgcgaca cggttttttc gggaatccgc gcaactttgg cgatgacggt ttcaatttgt
     3181 gctttgccga tgactttttt ctgtttggat ttgggcagaa tccgttgcgc cgcgcctgct
     3241 tcgtccatca cgtcgatggc tttgtcgggc aggaaacgct cgttgatgta gcgtgcggag
     3301 agttcggcgg cggcttcgag tgcgccttgc gtatagcgga cttggtggaa ggcttcaaac
     3361 atcggcttca agccgcgcag gatttgtacg gtttcggaaa cggtgggttc gaccacgtcg
     3421 attttttgga agcggcggct taaggcgtgg tctttgtcga aaatggtgcg gtattcgtcg
     3481 taagtggtcg cgccgatgca gcgcagcgaa ccttttgcca gcgcgggttt gagcaggttg
     3541 gacgcgtcca tggtgccgcc gctggtgctg cctgcgccga tgatggtgtg gatttcgtcg
     3601 ataaacaaaa tggcgtgcgg gattttttcg agctgtttca agacggattt gacccgcgct
     3661 tcaaagtcgc cgcggtattt cgtgcccgcc aacagcgagc ccatatccag cgcgtacact
     3721 tcggcatctt tgagcgcgtc tggaatgccg ccgttgacga tttgatgtgc caaaccttcc
     3781 gccagcgcgg ttttgcccac gcccgcttcg ccgaccaaaa gcggattgtt tttgcggcgg
     3841 cggcatagga tttgcaccag ccgttccatt tcgtgtttgc gaccaatcaa agggtcgata
     3901 cggccggctt tgacttcggc gttgaggttg acggtgtacg ccgataaagg gtttttgccc
     3961 ggtttggtgc ggttttcatt atcgtcgtcc atgccgtctg aagaatagtt gccatcgtct
     4021 tcatcttcat cgggagagcc gtgggcaata cagcgcaaaa cttcaaaacg cgtaaccgat
     4081 tgcagcttga ggaaatagac ggtgtggctg tcggtttcgc tcatcagcgc gaccaaaacg
     4141 tccaacggtt cgactgcggc ttttccggca gactgggtat gcaccatcgc ccgttgcatc
     4201 acgcgttgga agccgagcgt gggccgggtt tcgaccgtgt ctaaaaggtg ttcgggaatc
     4261 aggggggtgt tttcggcaac gctggcggcg agctgttcgg acaccacttt caaatccgcg
     4321 ccgcagagtt ttaagacgtt cggcacggag gcatcttctt cgatgagtac caaaagcaga
     4381 tgctcgaggc tgataaattc ataatgagcc ttacgcgcct cgcggtaaag ctgctgcaaa
     4441 atctgttcca attcgggtgc aagcatatta aatctcctcg acaatacatt gcagcggatg
     4501 cccttcggct tttgcccgct gcatgacttg ttgctgtttg gtttgggcaa tatcgcgcgt
     4561 gtaagtgccg cacaggcctt tgccttcgtg atgaaccaag agcattaccg ctaccgcctg
     4621 ttcttgtccg agcatgaaga tttcggtcag gatttcaacg acaaattcca tcgtggtgta
     4681 atcgtcgttc aataggaaaa cgccgtaacg tttcggcggc agggtgttca gacggtgcaa
     4741 gagcgtgtcg gattggtgtt gcgcggtcat agtgtgtccc atttgaaagc cgcgttcaga
     4801 cggcattttt gttgtatttt cggtactttt gcctattttc ccactttttt gaaaacatag
     4861 cttgacgttt tgtcttaaca aatgtaaaaa gacggttaag caggattggg catccgccga
     4921 gtatatcaag ctggaaggaa cgccagacgg ctcggtttgc cgtatgcctt gttttgctga
     4981 ttttgttaat ttttgagtat aggaagtttc taatggcaac cggtatcgta aaatggttta
     5041 acgacgctaa aggttttggt ttcatcactc ctgatgaagg cggcgaagat ttgttcgctc
     5101 acttctcagc aatcaatatg gaaggtttca aaaccctgaa agaaggccaa cgcgtctctt
     5161 tcgacgtaac caccggccct aaaggcaaac aggccgccaa cattcaggct gcttaattcc
     5221 tgatgtacgg tcaaatgtat atttgaaaac ggcgggacag gcaatgtccc gccgtttttg
     5281 tctgccgttt tgccggcggc ggaaaaaccc caatccccgc acgccttatc ctgaacttgt
     5341 gtgtaccctg ttgtggacaa gtggcttagt attttgacgg ataagggaaa atcagtgctg
     5401 atgaaaaaat gtgcaatgtt gtcggcaaaa ggcggtgcgg cataaaacgg caaacgggca
     5461 ggcacggggc aaaacgtgct gccttcgtct tcagacggca tcggcagggc gttcagcttc
     5521 cggcaaccgt catccccgca atcagaatcg agccgatttt gttggacgaa cgccgcaaag
     5581 cgtcatccgc cacgccgaca atgtcgcggt acatatcctg caagcgtccg gctacggtaa
     5641 tctcgtggac ggggtaggca atcacgccgt tttccaccca aaaacccgcc gcgccgcgcg
     5701 agtagtcacc ggtaatggtg ttcgcgccct gtcccatcag ttcggttacc aacaatcccg
     5761 tccccatttc tttcagcagg tcggattgcg tttcgtgcgt atggttcaaa tacaggttgt
     5821 gcgcgccgcc ggcgttgccc gtggtctgca taccgagttt gcgcgcgctg taactgctga
     5881 ggaaatagcc tttgacaatg ccgttttgaa tcacgaagcg cggtgcggtg gcaacgcctt
     5941 ccgcatcaaa atagctgctg cggaaagagc gggggatgtg cggttcttcg cgcaggttga
     6001 ggaaatcggg caggactttt ttgccgatgc tgtcgatcag gaaactgctt tggcggtaga
     6061 gcgcgccgcc ggagagtgcg ccgacgaggt gtccgataag accgcccgaa acggtggtat
     6121 cgaagaggac ggggtagctg cctgtcggga tgctgcggct gccgagtcgg cgcaaagtgc
     6181 ggcgggcggc ggtttgaccg atggtttcgg ggctgtccat atccggatgg cggcaggcgg
     6241 aatcgtacca gtagtcgcgc tgcatcccgt tttcgtcggc ggcaacgacg ctgcaggaaa
     6301 tgctgtggtg cgtgccttgc cggtgtgcgg caaaaccgtg ggtgttgccg taaacgtatt
     6361 ggtaatggcc ggtttgcacc gccgcgcctt cggagttttc gatgcgctca tcctcgttca
     6421 gggcggcttg ttcgcattgt tttgccaact cgacggcggc ttccgtatcc aaatcccatt
     6481 cgtggtaaag gtcggggtcg ccgatgtgtt ttgccatcag acaggcatcg gcaagtccgg
     6541 cgcaaccgtc ttcggcggtg tggcgggcga tgtcggcggc ggcgcggacg gtgtcgcgca
     6601 gggcttgttc ggagaaatcg gcggtgccgg cgcggccttt gcgtttgccg acgtaaacgg
     6661 taatgtccag cgacttgtcc tgctgaaact cgatttgttc gatttcgccc agccgcacgc
     6721 tgacgctttg tcccaatgat tcgctgaaat cggcttcggc ggcggttgcg cccgtcgctt
     6781 ttgccaagtc gagcgtgcgg cggcagaggt cgaggagttc ggaagcggtg tggttgaaca
     6841 gcatagaaat ctttcttgat gatgctttgc ggcattttaa ccgtttcggg cggcaggggc
     6901 aaaagcgcgc cgtttgcagg gcggacggtg caaaatgccg tctgaacgcg gcggcattct
     6961 gttaaaatgc gctattggaa aaattcgaga atcaagatgt ttgaacaaga agacgaatgg
     7021 atcagcaaaa cccaaatgaa aaagcagatg aacgatttgc aggatttggg tatggcgttg
     7081 accaagctct caaacgatac gctgaaaaaa atcggtttgg atgcggattt gtacgaggcg
     7141 gtaaccgcct ataaaaaaat cacatccaac ggcgcgctca aacgccaggc acaatttatc
     7201 ggcaggctga tgcgcgatac cgatcccgcg cccatcgagg cgtttcttgc caagctgcgc
     7261 ggcgacgatg cggcgcacaa cgcctttttg caacgcgtgg aacaggcgcg cgtacggctg
     7321 ttggcagacg acggcgcgtt gacgcagttt atgtcggatt ttccgcatgc ggacgcgggc
     7381 aagctgagga cgctcgtccg caacaccaaa aaagagcagg agcaaaacaa accgccgaaa
     7441 aacttccgcg cgctgtttca agaattgaaa accgtgatgg aaaacgggga cgcggaaatt
     7501 taggcatatt ttcagacgac atccg
//
LOCUS       DAWHRA010000099         7445 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWHRA010000099 DAWHRA010000000
VERSION     DAWHRA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.099"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            734..2182
                     /gene="gnd"
                     /locus_tag="WIB30_001567"
     CDS             734..2182
                     /gene="gnd"
                     /locus_tag="WIB30_001567"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ3797611.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2245..3516
                     /gene="waaA"
                     /locus_tag="WIB30_001568"
     CDS             2245..3516
                     /gene="waaA"
                     /locus_tag="WIB30_001568"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ3797612.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMTECRKSGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3557..4006
                     /locus_tag="WIB30_001569"
     CDS             3557..4006
                     /locus_tag="WIB30_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ3797613.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4039..5013
                     /locus_tag="WIB30_001570"
     CDS             4039..5013
                     /locus_tag="WIB30_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ3797614.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5082..5157
                     /locus_tag="WIB30_001571"
     tRNA            5082..5157
                     /locus_tag="WIB30_001571"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5115..5117,aa:Lys,seq:ttt)
     gene            5377..6630
                     /gene="murA"
                     /locus_tag="WIB30_001572"
     CDS             5377..6630
                     /gene="murA"
                     /locus_tag="WIB30_001572"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ3797615.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6711..>7445
                     /gene="pgk"
                     /locus_tag="WIB30_001573"
     CDS             6711..>7445
                     /gene="pgk"
                     /locus_tag="WIB30_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ3797616.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKK"
BASE COUNT         1731 a         1848 c         2138 g         1728 t
ORIGIN      
        1 tgcgcgggaa tgacggcgga gcggtttctg ttttttccgg taaattccta aaactcaaaa
       61 tttcatcatt cccactttcg tgggaatgac ggaatgtagg ttcgtgggaa tgacgcggta
      121 caggtttccg ttacgtatcc ccatccgaaa gtttacttat acacaagcaa tccaaagtta
      181 tctgactgaa atattgtgag atttacccgc aaatctcatt ttttccacca cttatacaca
      241 aatccggatg cacactctgc cgatatgcag aaataagaca ttcgtgccga cacctgacca
      301 tttccacaag ttccaaacac gcttccggct taaatctcca cagtattccc gttcttaaag
      361 gcgccagcag ccattcggta aagaaaagta ggatgccatc tgcacaacaa gcaaactatc
      421 cacaaaactt attccccata aaaaacaatc cacaggtatg cacaaaaggg aatccgcctt
      481 accgacaccc taaaaaaagg taatcctgat gatttttaaa ataaaaaaaa gttacccaca
      541 gaaaatttct cctatcaata taatcatcgt ttttttattt aatttttata ttttatttat
      601 aagccttcag ggaaacgcac ggtaaagcgt aaaggcatct tttcaaaaaa ttaaagagtt
      661 aaggattgtt aaacaagtgt aaatcaggta gagttgcggt cgataaatta aatctataac
      721 ggagcttcct gcaatgaaag gcgatattgg cgtaatcggt ttggcggtta tggggcaaaa
      781 cctgattttg aatatgaacg attgcggatt taaggttgtc gcctacaacc gaacaatcgg
      841 taaggtggac gaatttttaa acggcgcggc aaaagggacg ggcattatcg gcgcgtattc
      901 cctgcaagac ttggtcgata agctggcaaa accgagaaaa atcatgatga tggttcgcgc
      961 aggttcggtc gtcgacgact ttatcgaaca actgctgccc ctgttggacg aggaagacat
     1021 cattatcgac ggcggcaatg ccaattaccc cgacacgaca cggcggacac attaccttgc
     1081 cgaaaaaggg attttgtttg tcggggcggg cgtatccggc ggcgaagaag gcgcgcggca
     1141 cggcccgtcc attatgccgg gcggggataa gcgcgcttgg gaagccgtca aaccgatttt
     1201 tcaggcgatt gccgccaaaa cgccgcaggg cgaaccgtgt tgcgactggg tcggcaagga
     1261 cggcgcgggg cattttgtca aaatggtgca taacggcatc gaatacggcg atatgcagtt
     1321 gatttgcgaa gcgtaccagt ttatgaaaga tggtttggga ttgtcctacg atgaaatgca
     1381 ccgcgtgttt gccgagtgga acaaaaccga gctggattct tatctgattg aaatcacagc
     1441 ggcaattttg ggctataagg acgaaggcgg tgaaccgctg gtcgagaaaa tcctcgatac
     1501 ggcagggcaa aaaggcacgg gcaaatggac gggcatcaat gccttggatt tgggtattcc
     1561 gttgacgctg atttccgaag cggtattcgc ccgctgcgtt tcgtcgttca aagaacagcg
     1621 cgtgcagacc ggcaagctgt tcgcccgaac cgttacgccc gtcgaaggcg gcaaacaaga
     1681 atgggttgag gcgttgagac aggccctgct tgcatccaaa atcatttcct acgcacaagg
     1741 ctttatgctg atccgcgaag cgggcgaaag ctacggctgg gatttggact acggcaacac
     1801 tgcgctgctg tggcgcgagg ggtgcatcat tcgcagcgca tttttaagca atatccgcga
     1861 tgcctatgaa aacaatcccg atttggtgtt cttgggtgcg gatccgtatt tcaaaaatat
     1921 tttggaaaac tgcctgccgg cgtggcgcaa ggtggttgcc aaggcggtcg aatgcggcat
     1981 ccctatgccc tgcatggctt cggcgattac gttcttggac ggctacacga ccgaacgtct
     2041 gcctgccaac ctcttgcagg cacagcgcga ctacttcggc gcgcacactt acgaacgcac
     2101 cgacaaaccg cgcggcgagt ttttccatac caactggacg ggcaagggcg gagataccgc
     2161 ttcgacaacc tacgatattt gattccgttg ccgaaatgcc gtccgaaccg ttcggacggc
     2221 atttgttttg caggaaatag aaagatgttc caatggcttt atgatgtatt gtggctgctt
     2281 gcgccgatat ggatacggcg ttatttggac aaacgctccg gaagtgcccc ggcatatcgg
     2341 gcgcatcggg acgagcgttt cggcaagccg tatccgaatc ccgttaccgg agcggtttgg
     2401 atacacgccg tttcggtcgg agaaacgcgt gccgcccagc ccttgatacg cgagttgcgg
     2461 cagcgttttc ccgatgcgcc gctgctgatg acgcagatga ccccgacggg acgggaaacc
     2521 gcgcaagttc tgtttcctga tgcacaatgc cgttatcttc cgtatgacaa aaaaacgtgg
     2581 gtacggcagt ttttgcgcga acaccgcccg atgttcggca ttttgatgga aaccgaaatc
     2641 tggcccaacc tgatgacgga gtgccggaaa tcgggcgttc cgctgttttt ggcgaatgcg
     2701 cggctgtcgg aaaaatcgct aaacggttat ctgaaagtcc gccgcctgat ccgtcctgcc
     2761 gccgcttcgc tgacgggttg tctggcgcag acggaggcgg atgcggcgcg gttggcgaaa
     2821 ttgggcgcgg catccgtgca ggtgtgcggc aataccaaat acgacctaat gccgtcggaa
     2881 cagatgaaaa cgctggcggg gcagtttgaa aaacgcatcg gaggcaggcc ggttgccgtg
     2941 tgcggcagca cgcgcgttta tcggggtgaa gacgaggcgg aaaaactgct ggcggcgtgg
     3001 caacaatatc gcggcgatgc gctgctggtc gtcgtgccgc gccatcccga gcattttcag
     3061 acggtatttg aaacggcaaa acgcttcggg tttaaggttc agcggcgcag cgacggtttg
     3121 ccggtcgaac ctgatacgca ggtgtggata ggcgacagta tgggcgagct gtatgcgtat
     3181 tacctgtgcg ccgatgtcgc ttttgtcggc ggcagtctgg tcgattcggg ctgtcagaac
     3241 attatcgaac cgctttcctg cggcgttccg acgatattcg gcttttcaac ctacaatttt
     3301 tccgaagcct gccggcacgc cttggcatcg ggggcggcgg ttcgggttga gtcggcggac
     3361 gcgtggcggg aagccgttga aaaaacctta tcgtccgagg ggggcggaat gcagatgcag
     3421 gcgcacgtgg acggctttat cgcacaacat cgcggagcgg gcgcgagaat cgccgaggcg
     3481 gtacgggaag cggtatgcgg acatcgggga cgatagtgat acaatccgta tccgagtttt
     3541 ccggttggag catcgtatga gtatttatgc cgtcgcgcac atcgtccacc tgtattgcgc
     3601 cattgccttt gtcggcggcg tgttttttga agtgctggtt ttgtccgtcc tgcatacggg
     3661 acgggtgtcg tgcgaggcgc ggcgcgaagt ggaaaaggca atgtcttacc gcgccgtcag
     3721 ggtgatgccg tttgtggtcg gactgctgtt cgccagcggc gtcgtgatgg cggcaaaccg
     3781 ctatctttct atattgggcg aaccgtttgc cacttccttc ggtacgatgc tgacgctgaa
     3841 aatcctgttg gcgttcagcg tgttggcgca cttcgccatc gccgtcgtca aaatggcgcg
     3901 ttccacactg accgtcggct ggtcgaaata catacacgcc gtcgtcttta cccatatgct
     3961 gctgattgtc tttttggcaa aagcgatgtt ttatatcagc tggtaacaca attacatttc
     4021 aaatcaggag attgaaccat gacagaatat cccggcatcg ggtcgccgtt gttttacggc
     4081 gttttttttg cggcagtgct ggtcatgatt gccttggata tgttttcgct gaagaaaaac
     4141 ggcagccata aagtcggcgt caaagaagcc ttggcatgga gcggcttgtg ggttgccgta
     4201 tcctgcctgt tcgcgggctg gctgtatttc gaactcgccg gcaatctcgg ctacggcgcg
     4261 gcagtcgcaa aagaaaaagt attggaattc tttaccggct acattttgga aaaatcgttg
     4321 gcagtcgaca atattttcgt gttcctgatg atattcggct acttcaaagt cgcaccacag
     4381 tttcagcacc gcgtgctgct gtacggcgta ttgggcgcat tggtattgcg cactgtcatg
     4441 attttcgtcg gcgcggcact ggttcagcag tttgagtgga ttctgtatct gtttggcgcg
     4501 ttcctgctct atactggtat acacatgatg aagcccgaag gcgatgagaa agaggatttg
     4561 gcaaacagca ggctgctgaa tgccgtcaag aaagtcgttc cggtcggcac ggaatttcac
     4621 ggcgagaaat tttttaccgt cgaaaacggc aaaaaaatcg ccacgccgct gtttttggtg
     4681 ctggtcatga ttgaattgag cgatgtcgtg tttgccgtgg acagtattcc cgccgtcttt
     4741 gctgttacca ccgatccgtt tatcgtgctg acctccaata ttttcgctat tttgggtttg
     4801 cgggcgatgt atttcctgct ggcggatgtg gcggaacgct ttatcttcct gaaatacggc
     4861 ttggcattcg tgttgagttt tatcggtgtg aaaatgctgg taatgcattg ggtgcatatc
     4921 ccgatttccg tttcgctgtc ggtcgtgttc ggcgcgttgg gtgcatcgat actgacctcg
     4981 ttaatttata cgaaaaaaca gcctgataaa taaaatgccg tctcaacgcc gacaaaatgg
     5041 ttgacagcat caggcttgat gtatagaatt tcgagcttgt cgggtcgtta gctcagccgg
     5101 tagagcagcg gacttttaat ccgttggtcg agcgttcgaa tcgctcacga cccaccagat
     5161 aaaacaaaaa aagctgagtt ttcaaaactc ggcttttttt gttttccgac aatccttgtt
     5221 cggaaagcgc ggcatttcgt caaattcttt ggtgcggctt ccgtattttt cccgaaaatc
     5281 ccttataatt ccgcctctgc aaccttcatc gggtgatgct ttatgcgttg tcccgagaat
     5341 tgaaatatta caagtaccca ccaggaaaga acaaacgtgg acaaactgaa aatctccgca
     5401 aacggcccgc tcaacgggga aataacggtc tcgggcgcga aaaacgcggc attgccgctg
     5461 atgtgcgcgg gtttgctgac atcgggtacg ttgcgcctga aaaacgtccc tatgctggca
     5521 gatgtcaaaa ccacgcaaaa actgcttcag gggatgggcg cgcgcgtcct gaccgacaat
     5581 atcagcgaat ttgaaatcaa cggcggtacg gtcaacaaca cgtgcgcccc ttacgagttg
     5641 gttcggacga tgcgcgcttc gattttggtg ctgggcccga cgctggcgcg tttcggcgag
     5701 gcgcaagtca gcctgccggg cggctgcgcc atcggttcgc gccccgtcga tcagcatttg
     5761 aaaggcttgg aagcgatggg ggcggaaatc gtcatcgaac acggttacgt caaggctaag
     5821 ggtaaactca aaggtacgcg cgtggcgatg gatgtcgtta ccgtcggcgg cacggaaaac
     5881 ctgctgatgg cggcgacgct ggcggaaggt acgacggttt tggaaaactg cgccatcgag
     5941 cctgaagtgg tcgatttggc ggaatgcctg gttaaaatgg gcgcgaaaat cagcggcatc
     6001 ggaacatcga ccatgatcgt ggaaggtgtg gacgagctgc acggctgcga acacagcgtc
     6061 gtccccgacc ggatcgaggc ggggacgttc ctgtgcgcgg tggcgataac cggcggcagg
     6121 gtggttttgc ggaatgccgc cccgaaaacg atggaagtgg tgttggacaa actggttgag
     6181 gcaggtgcgg tgattgaggc gggcgacgat tggattgcca tcgatatgcg gcagcgtccg
     6241 aaggcggtgg acatccgcac ggtcgtccac cccggcttcc ccacggatat gcaggcgcag
     6301 tttatggcat tgaatgccgt ggcagaggga agctgccgcg tggtggaaac gatttttgaa
     6361 aaccgcttta tgcacgtccc cgagttgaac cggatggggg cgaacatcac aaccgagggc
     6421 aatacggcat ttgtgcaggg tgtggaacgg ctttccggcg cagtcgtcaa ggcgacggat
     6481 ttgcgtgcgt ccgcaagcct cgttatcgcc ggtttggcgg cgcgcggcga aaccgtggtc
     6541 gaacagattt accacttgga tcgcggttat gaaaatattg aaaaaaaact cggcagcgtc
     6601 ggcgcgaaaa tcgagcgcgt atccggctga tcgggttgcg atgccgtctg aagccttcag
     6661 acggcatcaa ttgtttctgt atcgcaaaca ctgattgaaa aggactgatt atggcatttt
     6721 taaaactgac cgaacaaaac gtgcagggca aaaccgtcct catccgcgcc gatatgaacg
     6781 tgccgttcaa agacggcaaa atcagcgacg acacccgtat ccgcgcctcg ctcgcgtcca
     6841 tccaatactg catggacaac ggcgcatccg ttatcgtgat gacccacttg ggccgcccga
     6901 ccgaaggcga gttccacccc gaagacgacg ttgcgcccgt tgccgcgcat ttgggcggcc
     6961 tgttgggcaa aaacgtgaaa gtattgaacg actggcgcga aaacaaaccg gctttgaacg
     7021 cgggcgatgt cgtgatgctg caaaacgtgc gtatcaacaa aggcgagaag aaaaacgatt
     7081 tggaactggg caaagcctac gcttctttgt gcgacgtgtt cgtcaatgac gcgttcggca
     7141 ccgcgcaccg cgcccaagcc tcgaccgaag ccgtcgccca agccgctccc gttgcctgcg
     7201 ccggcgtatt gatggcgggc gaactcgacg ctttgggcaa agccttgaaa cagcccgcgc
     7261 gcccgatggt tgccatcgtt gccggcagca aagtgtccac caagctgacc atcctcgaat
     7321 cgctggcgga caaagtcgac caactcatcg tcggcggcgg cattgccaac accttcctgt
     7381 tggcggaagg caaagccatc ggcaaatctt tggcggaaca cgatttggtg gaagaatcca
     7441 aaaaa
//
LOCUS       DAWHRA010000100         7436 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWHRA010000100 DAWHRA010000000
VERSION     DAWHRA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.100"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(172..384)
                     /locus_tag="WIB30_001574"
     CDS             complement(172..384)
                     /locus_tag="WIB30_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3797617.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAKPD
                     TETRRTDMSLEKWRQFLDWTEKTNAV"
     gene            complement(396..677)
                     /locus_tag="WIB30_001575"
     CDS             complement(396..677)
                     /locus_tag="WIB30_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797618.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(715..978)
                     /locus_tag="WIB30_001576"
     CDS             complement(715..978)
                     /locus_tag="WIB30_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797619.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(998..1570)
                     /locus_tag="WIB30_001577"
     CDS             complement(998..1570)
                     /locus_tag="WIB30_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEZ3797620.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVL
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1549..1860
                     /locus_tag="WIB30_001578"
     CDS             1549..1860
                     /locus_tag="WIB30_001578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797621.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(1882..2757)
                     /locus_tag="WIB30_001579"
     CDS             complement(1882..2757)
                     /locus_tag="WIB30_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ3797622.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2802..3092)
                     /locus_tag="WIB30_001580"
     CDS             complement(2802..3092)
                     /locus_tag="WIB30_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ3797623.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3104..3349)
                     /locus_tag="WIB30_001581"
     CDS             complement(3104..3349)
                     /locus_tag="WIB30_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ3797624.1"
                     /translation="MINHECINQPYGRILKFSRVQAKEMLTADELKENFAKNGQTLAQ
                     WARENGFKPRDVYLVIGGQRKGNYGKGHEIAKKLGLK"
     gene            complement(3342..3566)
                     /locus_tag="WIB30_001582"
     CDS             complement(3342..3566)
                     /locus_tag="WIB30_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ3797625.1"
                     /translation="MEKPLNFKPIPYPQTRESAAGWFKRNGVCKAHWAKYFNLERTTV
                     EHLLRGKLKGNFGKSHEAAVLLGMKEKSDD"
     gene            3838..4209
                     /locus_tag="WIB30_001583"
     CDS             3838..4209
                     /locus_tag="WIB30_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3797626.1"
                     /translation="MNNSSFFGNRLKEERKKLKMTQAEIAEKCGVSGRMWGDYERGIS
                     QPKAELFFQFEKVGIDVQYVMHGRRGETAVMPSETLNAEEQELLVLFREAAAADREMI
                     LMVARRAEKKAQTALGKVSNG"
     gene            4211..4834
                     /locus_tag="WIB30_001584"
     CDS             4211..4834
                     /locus_tag="WIB30_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797627.1"
                     /translation="MDQFELCQKEHVNPFALSKQYLLVVTFVKSSSKNFQAALLWARS
                     AKLFENLEIGKETIYCCAFDKTAEQAGMAGVFLNYIENWNGKQIYINGRIHSGSIYDL
                     LGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDLTGKKKETSSAKKFVM
                     PCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENFRQYE"
     gene            4827..5021
                     /locus_tag="WIB30_001585"
     CDS             4827..5021
                     /locus_tag="WIB30_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3797628.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            5045..5608
                     /locus_tag="WIB30_001586"
     CDS             5045..5608
                     /locus_tag="WIB30_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797629.1"
                     /translation="MKILALLIAATCALSACGSQSEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSAANKGLNDQKTGLTLPEHVVPIDNAEGKNLLHDFSDGLTILTVDTDKADKIT
                     AVRVVWNTDAMPQKAEKLSKAAAALIAATAPEDRTMLRDTGDQIEMAIDSHNAQKEPT
                     REWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            5639..6586
                     /locus_tag="WIB30_001587"
     CDS             5639..6586
                     /locus_tag="WIB30_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797630.1"
                     /translation="MMIEKSISIVDGKEYSVFAVSHEFRYTFDEPILVADLISSLKAY
                     ETLTSSYLPAILNQLFDVKIQKIKVAVSEIERGSFLEKLIFNLFFKDEDAYNEFCLKI
                     RKFLGTENQDGSINMSKIIMFAMTTLLGVGAGYLLFKNPPQEKQAITNNIVTVINADS
                     SVALDGEHLVSVVKEVTGSSKQKTAENVAKVYAPASKNNGSITLGTDDVRIEPVAQQT
                     VATLPKDVDLRDTPLTEDYTDIDVQIRATDRDKNSGWYAVIDQIVPSRVRLELPEDID
                     LNRLANNATIRANVTVEFDLKQNGSRKPKKIILTSLSTD"
     gene            6671..7174
                     /locus_tag="WIB30_001588"
     CDS             6671..7174
                     /locus_tag="WIB30_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ3797631.1"
                     /translation="MSDKFNQFINRVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGVIGAVSLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            7188..7361
                     /locus_tag="WIB30_001589"
     CDS             7188..7361
                     /locus_tag="WIB30_001589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797632.1"
                     /translation="MSKKSLIALMTAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRHK"
BASE COUNT         1793 a         1793 c         1844 g         2006 t
ORIGIN      
        1 tgggtttcgg ctgtcgggag ggaaaggaat tttgcaaagg tctcagagtg agtttatttt
       61 ggggcggcgg caggtcgggg gcaagcggcg tggggcttgg ttgtggtttt taggtttggg
      121 ggtgggtaaa aaatgccgta cccggcgggc aggcttcgca ctctgaaggt ttcagacggc
      181 gtttgttttt tctgtccaat cgaggaactg ccgccatttt tccagcgaca tatcggtccg
      241 ccgcgtttcg gtatcgggtt tggcttccca gcggcctacc tgtatgtagt gcttcacccc
      301 gacgatttgc gccaactcgg cctgtgtcag tttgcagcgg ttgcgcaggg tgcgaaggtt
      361 gtaaggcgtg tagccgagtt ccatgtcgtt gcggtttatt ttgtttcgca tatttttttg
      421 actgcccggc ggcaggtttc ggtaaggacg gcggcaaatc gggcttcgtc tgccggtttt
      481 ccgtcgaaaa aaataaaatc gtaaagggtg atgccgtttt cggaatggcg gtaatgcccg
      541 cccgggcatt cgctgtctgt ctgcctgtgg attttggcaa tcaggcgggg atagcggcaa
      601 tgggtaatga aacggcttgc gccgtcttgc ccgataatcc attcggggta gcggttgaat
      661 agtgcggctc tgttcatttt gttcggggga taaagcccct cgcggggctt gtggtcaggc
      721 aaatttgaat atcagtgcca acacggcggc gatggcggta accagcccgg tggccgccat
      781 catgggatac catcgggatt cttgggctat ttttacggat tcggcgttga ttttgtgcgc
      841 gtctgcaatg attttggcga tttcggcatc tacttttctc agactggagt gtttcatttc
      901 ttgttcgatt atgttcatcg tacttccttt cgtttttggc ggttgccgcc gcttgtcgga
      961 tggtaggatg tctgccatgt gtatatattg atacctttta ggttttattg caagtgtttt
     1021 gggcggcggc ttcgtatgct tggcggtggc ggcggctgta ccattcggcg agttcgcgcc
     1081 gctcttggag gcggtagctg tcggcggtca ggtagtggtg caggcttgag aagccggcgc
     1141 gttggagggc ggcgcggctg atgtggccga gggcgaggtc tgttgtgagg gtgtcttttc
     1201 cggcggtcag gctgatgttg gtgaagaggt ggcggaatcc gtgcattgtg tgtttggatt
     1261 tgccgggggt gctgccgtca tagcccagtc gtcggatggc gttgtgggcg aatttgatgc
     1321 tgatgtggtc gggatggggg gcgggtttgc gccgtgggcg gatgccgggg aaaaggtgga
     1381 tgttgtcgcc ggtctgtgtg tgcagctctc ggagtatttc taccgcccag tccgacagta
     1441 ggacggtaaa ggggtgtttg gtcttcatgt cggcggcggg gatgtgccat aaccgggcgg
     1501 tgaggtcgat gtcttgccag cgggcggaaa gcagtgcgga cggacgggat gacttccccg
     1561 gtttccccat caatccgcgc cgcctcgatt tttccgttgg cgcgcagcat ttccaccgcc
     1621 tgccgcaccg acatcaaccg cttgccgttg cgcctcatcg cctccaccaa aaccgccgaa
     1681 atcaatttgg cttcttccgg tttaagctcc gtcttgcccg catcgctgcg ccgtttgcgc
     1741 gtcggcttga cgctgaccgc ctccagcttg cggtatagcg tggcaaggct gatgcccaat
     1801 tcctgcgcct gctgcttaag atatgcagag cgtgcgccgc gttccattgc ttccgcctga
     1861 ttctcgactg ccttaagacg ctcaatcatt gccggattca tcgccttctc ccgtttcacc
     1921 gcccaaccat tccggcacat tgtctgtcgg tgcttcagtc ggcagggcat agctttcgcg
     1981 cagttgctcg cagtccaaaa taatttgatt gagcgtgccg accatctttg cctgatggct
     2041 gatcccgtgt gcctcactgt gcgcattaag ttggtcgaac aaatctttca gacggctcac
     2101 ttgactgcgg ataccgacct caaggcttgt taactgcatc gccaactcgc tgcctacgtc
     2161 ttccgccttc ggctctctga caacggtttg cttcttagcc agcttctcgg ccagctcatc
     2221 aattttggct gttttggttt tcatcacttc gtctttggcg gcgaggtttt cgcggctttc
     2281 gcgcagggcg acgcgcagct cgcgcaccgt cattcggtcc acatcgtcaa aggtcatgcc
     2341 gttgacttct tccccttcgg ccaaacccac cagcgtaacg tcttcttcga ccagcagctc
     2401 aagcagcttc gacttgccca aatccatcag cttcggcgcg gctttctgca tttgcggggt
     2461 cgcaaagcgg cgagtggctg acatcagacg tgatgtttct gcgataccca gcccaaattg
     2521 gcttttcaca atttccataa accgtccgtg ttccgtatgc tcttttaaaa taatcagcgc
     2581 acgtcccagt tcgaacatgc cttccatcgt ctgccgtact gcttgacgac cgcgttcaac
     2641 ccaacgctct tcgctataag tctcgccgtt accccactgc tccatcacca gcacactgcg
     2701 catcgctgcc tgattgctta ctttgtcggt tgcgataatt tcaatttctg tattcatttt
     2761 ttatctccaa agtttccgac gtcggaaact ttcaaaatcc gttaatccac atcgacccgc
     2821 ttgccgattt cggcaatctt gctttgcagc cgttcatgct gctgcctgaa ccgctctgcg
     2881 atttgcaggg ttttgatgcc gtaggcgtag ttgccgtttt caagtttgat gaccaatccc
     2941 gaggcaacca aatcatcaat atccctgctg acttgcgatg gcgtcagccc cagtccgacc
     3001 gataaatcct tattgctcag accgataatc ggatgctcgt caagcgcgat aaagaccctc
     3061 aatagccgtt gtaccctttt actttctgcc atccgcatcc tccttatttc agtcccagct
     3121 tcttggcaat ttcgtgcccc ttgccgtaat tgcctttgcg ctgtccgccg atcaccagat
     3181 acacatcgcg cggcttaaag ccgttctctc ttgcccactg cgccagcgtc tggccgtttt
     3241 tggcaaaatt ttctttcaat tcgtctgccg taagcatttc tttggcctgt acgcgggaaa
     3301 atttcagtat ccgtccgtac ggttggttaa tacactcatg attaatcatc gcttttctcc
     3361 ttcatcccca gcaaaaccgc cgcttcgtgg cttttgccaa aattgccttt cagcttgccg
     3421 cgcaagaggt gctccaccgt ggtgcgctcc agattgaaat atttcgccca atgcgccttg
     3481 cacaccccgt tgcgcttaaa ccacccggcc gcgctctcgc gcgtttgcgg atagggaata
     3541 ggcttgaaat tcaaaggttt ttccatatta tttatgcctt tcgtgtgata taatgtgttt
     3601 gtttttaggt ctctttgcac gataaacatc cggtctgttg tgcagcagca gggtcttggc
     3661 ttgttttaaa gtaaacaagc tttttctcgt agctgggtgt atgtgatcca tcatgagctg
     3721 tcgcgcatga gccttcaaag tgttcatttt ttcgtccttt ctcgtgatga tttagggtgt
     3781 ttgtgtttcg atgtggaaat tataggaaga attcttccta ttttgcaagg aatatttatg
     3841 aataactctt ccttttttgg caaccgattg aaagaagaaa gaaaaaaatt aaaaatgact
     3901 caagctgaaa tcgctgaaaa atgtggggtt tcaggaagaa tgtgggggga ttatgaacgt
     3961 ggcatcagcc agccaaaagc ggaacttttc ttccaatttg aaaaggtggg tatagacgtt
     4021 caatacgtca tgcacggcag acgcggcgaa acagcggtca tgccgtctga aaccctgaac
     4081 gccgaagaac aagaactgct ggtcttgttc cgcgaggcgg cagctgccga ccgtgaaatg
     4141 attctgatgg ttgcgcgcag ggcagagaaa aaagcccaaa ctgcgcttgg taaagtgagt
     4201 aatggataaa atggatcaat tcgaattgtg ccaaaaagaa catgtcaatc catttgcctt
     4261 gtcaaagcaa tatctattgg ttgtaacatt cgtcaaatcc agcagtaaaa attttcaggc
     4321 agcattactt tgggcaagaa gtgccaaatt atttgagaat cttgagattg gaaaagaaac
     4381 catctattgc tgcgctttcg ataaaacagc agaacaggct gggatggccg gggtattttt
     4441 gaattatatt gaaaattgga atggcaaaca gatttacatc aatggccgaa tccatagtgg
     4501 cagtatttat gatttgttag gggttttaga ctgctatcaa aaatcacagt cctgtcccaa
     4561 ccctaaaagc cactgttgct ttgtttcaga cgacattttt ctatggcatg gatcaagacc
     4621 aacgtttgaa atcagtcttg atctaactgg aaagaaaaaa gaaacatcct ctgcaaagaa
     4681 atttgtgatg ccttgtatta atttccgtca ccataggatt gaaaaagaaa cctacttagg
     4741 aaattggaat gaacaaattg ccgcattggc agtaaaacaa aatatagatt ggtgtccaag
     4801 ttttgatatt gagaatttta gacagtatga ataattacta tctatatagg aattgcagca
     4861 gcgatgtgtt atgggtcaaa cgtatccaac gccaaatcga cggcagccta ctcttgattt
     4921 ctgacaattc aacctatcca cccatgccct tggcactggc ggaacacccc gatattcaaa
     4981 tcatcgggca ggtagtgcag gtatcaaaag acttgaacta gacacaatca aaaagggaaa
     5041 tagaatgaaa atactcgctt tattaattgc cgctacctgt gctttatctg cgtgtggcag
     5101 ccaatctgaa gaacaaccgg catctgcaca accccaagag caggcacaat ccgaattaaa
     5161 aaccatgccg gtaagctata ccgactatca atcagcagcc aataaagggc tgaatgacca
     5221 aaaaaccggt ctgacccttc ctgaacatgt tgtccctatc gacaatgcgg aaggaaagaa
     5281 tctgctgcat gacttttcag acggcctcac aatcttaacc gttgataccg ataaagccga
     5341 caaaattact gctgtccgag tagtctggaa tacagatgca atgcctcaaa aagcggaaaa
     5401 actgtccaaa gctgccgcag ccttgattgc ggcaaccgct ccggaagacc gcacaatgct
     5461 gcgtgatacc ggcgaccaaa tcgaaatggc gattgacagc cataatgcgc aaaaagagcc
     5521 aacccgagaa tgggcgcgtg gtgggattgc ttataaagtc actgttacca atttaccgag
     5581 cgtggttttg acggcaaaag ctgagtaaat ctattaagta gaaaaaatag aaagggaaat
     5641 gatgattgag aaaagtattt ctattgtaga tggaaaggaa tactccgttt ttgctgtatc
     5701 acacgagttt cgttatacct ttgatgagcc tattttagtc gctgacttga ttagttctct
     5761 aaaagcttat gaaacactga caagtagtta tcttccagca attttgaatc agctgtttga
     5821 tgtcaaaatc caaaaaatca aagtagctgt atctgaaatt gaaagaggat ctttccttga
     5881 aaaactgatt ttcaatttat tcttcaaaga tgaagatgct tataatgaat tttgtcttaa
     5941 aatacgaaaa tttctaggaa cagaaaatca ggacggaagt attaatatgt ccaaaatcat
     6001 tatgtttgca atgactacac ttttaggggt aggtgctggt tatctcttgt ttaaaaaccc
     6061 gccacaagag aagcaggcaa taaccaacaa catcgttacc gtcattaatg ctgatagttc
     6121 tgtcgcactg gatggtgaac atttggtttc agtggtaaaa gaagtaacag gaagcagcaa
     6181 gcaaaaaact gcagaaaatg tggcaaaagt atatgctcca gcaagtaaaa ataatggcag
     6241 tattaccctt gggacagatg atgttcggat tgaacctgtt gcacaacaaa ctgtagcaac
     6301 tttgcctaaa gatgtggact tacgtgatac gccattgact gaagattaca ccgatattga
     6361 tgtgcaaatt cgtgctactg accgtgataa aaattcaggg tggtatgcag tcatagacca
     6421 aattgttcca tcacgtgttc gattagaact gcctgaagat attgatttga ataggctggc
     6481 taacaatgct actatccgtg caaatgtaac agttgagttt gacttaaagc aaaatggctc
     6541 tcgtaagcct aaaaaaatca tcctcacatc tctctctact gattaagttt taacccgtat
     6601 taaaggctta gtcagacggc ctttcctaca atccctgtat tgatttttaa ttcaatacag
     6661 ggatttttcc atgtcagaca agttcaacca attcatcaac cgcgtcctct ctcacgaggg
     6721 tggttacgcc aaccatccca aagaccccgg cggcgaaacc aattggggca tcactaagcg
     6781 caccgcacag gcaaacggct acaacggctc catgcgtgcc atgacgcgtg aacaggcaat
     6841 cagcatttac cgtaaagcgt tttgggagcg ttaccgcgcc gaccaaatgc cggaagcggt
     6901 cgcgttccaa ttttttgatg cctgcgtcaa ccacggttac ggcaatgccg cccgtatgct
     6961 gcaacgcgcc gcaggcgtac cggacgacgg cgttatcgga gcagtcagcc tcaaagccat
     7021 caattccctt cccgaaaacg accttttatt gcggttcaac gccgagcgtc tggtctttta
     7081 taccaagctc ggtacgttca cctctttcgg caagggctgg gtacgccgtg tggcgcaaaa
     7141 cctgattcac gcgtctgcag ataacactga ttaaagggag ataaaccatg tcaaaaaagt
     7201 cactcatcgc cctaatgacc gcagccatgc agcccgattt cagccacagc gacctaggca
     7261 ttcgctacgc catgccgact cagggatgtt ggacgcaagc ccaccgcaag agcggggtag
     7321 ccgccgcgaa acgcgcagcc aaaaaaaagc gtcacaaata accgcctttt tccgatggct
     7381 gggcggcttg gtctctaatc cggccacagg aaaaatcagc cataccaaac tatggg
//
LOCUS       DAWHRA010000101         7391 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWHRA010000101 DAWHRA010000000
VERSION     DAWHRA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.101"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(71..430)
                     /gene="rnpB"
                     /locus_tag="WIB30_001590"
     ncRNA           complement(71..430)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WIB30_001590"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(509..1612)
                     /gene="prfB"
                     /locus_tag="WIB30_001591"
     CDS             complement(509..1612)
                     /gene="prfB"
                     /locus_tag="WIB30_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ3797633.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1715..2608)
                     /locus_tag="WIB30_001592"
     CDS             complement(1715..2608)
                     /locus_tag="WIB30_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ3797634.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHIGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRRIQQFPIKFAAIATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2950..3399
                     /locus_tag="WIB30_001593"
     CDS             2950..3399
                     /locus_tag="WIB30_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ3797635.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3473..3781
                     /locus_tag="WIB30_001594"
     CDS             3473..3781
                     /locus_tag="WIB30_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797636.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAADKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3898..4740
                     /locus_tag="WIB30_001595"
     CDS             3898..4740
                     /locus_tag="WIB30_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ3797637.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFIEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            4730..5476
                     /locus_tag="WIB30_001596"
     CDS             4730..5476
                     /locus_tag="WIB30_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ3797638.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5498..6085
                     /locus_tag="WIB30_001597"
     CDS             5498..6085
                     /locus_tag="WIB30_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ3797639.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6091..6270
                     /locus_tag="WIB30_001598"
     CDS             6091..6270
                     /locus_tag="WIB30_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ3797640.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6502..6942
                     /locus_tag="WIB30_001599"
     CDS             6502..6942
                     /locus_tag="WIB30_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797641.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1904 a         1964 c         1675 g         1848 t
ORIGIN      
        1 aaacacaacc taaataaaaa tgccgtctga accatatttc aggtttcaga cgacatttgc
       61 gtgtcggatg cacaccggac aggcggtaag ccgggttctg tctcggacag tcattcctct
      121 aggcataccg ttaccggtat gctcaagcaa cctacccgaa cgctcggcgg gcagcgtcat
      181 tgcgttctgt ttggtcttgc tccgaatggg gtttggcctg ccgcatattg ttaccaaatg
      241 cgcggtgcgc ccttaccgca ccttttcacc cttgcctgtg ctgccaaagc agccatcggc
      301 ggttttgctt tctgttccac tttccgtcgc gttaccgcgc ccggccgtta accggcattc
      361 taccctgcgg agcccggact ttcctccccg tatgccttac gcgatacgcg gcgactgtct
      421 gcccgtcccg tgtgcggcgc ggattataac acgaaacgca aaaatgccgt ctgaaacggt
      481 acaggtttca gacggcatac agcctaaact acacgccctg tttcaggctg gcttcgataa
      541 agccgtccaa atcgccatcc aatacggctt tggtgttgcc gacttcgtag cctgtacgca
      601 agtctttgat acgtgaggaa tccaaaacat acgaacggat ttggctgccc caacctacat
      661 cggatttacc ttcttccaac gcctgtttct cttcattgcg tttgcgcatt tccaattcat
      721 acagtttgga cttcaacatt tccatcgcgg cggctttgtt ggcgtgttgc gaacggtcgt
      781 tttgacattg caccacaatc cccgtcggct cgtgggtaat gcgcacggcg gagtcggttt
      841 tattgatgtg ctgaccgccc gcgcccgatg cgcgataggt gtcgatacgc aaatcggcgg
      901 ggttgatttc gatttcaatg gaatcatcga tttcagggta aacgaacacg gaggcaaacg
      961 aagtatggcg tttgttgttc gagtcaaacg gtgaatagcg caccaagcgg tgaacgccgg
     1021 tttcggtacg cagcaaacca taggcgtatt cgccttccac tcggatagtc gcgcggttga
     1081 tgcccgcgat ttcgccgtcg tcttcctcaa ggatttcgat tttgaagcct ttgcgctcgg
     1141 cgtagcggct gtacatacgg aacagcatcc ccgcccagtc ttccgcttcc gtaccgcccg
     1201 cgcctgcggt gatgtcgata aagcagttgt tcggatcggc aggctggttg aacatccgtt
     1261 tgaactccaa atccgccatc tgtttttcca gccccgccac atcttcctgc acggcggcga
     1321 aaccttcttc gtcgttttct tcgacggtca tttcaatcag catacggttg tcttcgatgc
     1381 ccgaagcgat gttgtcgagc gtcaacacga tgccttcgag gattttgcgc tctttgccga
     1441 tttcttgggc gcgtttcggg tcgttccaaa gttcggggtc ttcggaaaga ccgacaactt
     1501 cttccaatcg gtctttctta ccctgataat ccatataaac gcggatgtct tcgctgcgct
     1561 tctccaagtc gttcagggtg ttgttgagct ggttgattac ttcggcttcc atgattcttt
     1621 tgttctttca aaattttagg ggcgtattgt acgggattcg ggtatttttt tctatggata
     1681 aagccttctg gaaacacgtt cggacggcat agcgtcaata acggtatgcc gccagtttgc
     1741 gtttgatttc aggcaatgcg gcacgtgcag cctcctcgcc caaccggatg gcgcgttttt
     1801 tctgatcgaa tcctccgact gcacccaaat ccaaaacctg cggtttgata accacatccg
     1861 cctgccccaa ctcgttttgc agtgcggaaa cgctcattac gttcagcgtc tgatcgagat
     1921 aagagaagaa gccttggctg atgtttttgc ccggacgggc ggaaatatcg acggcaatca
     1981 cgaaattcgc cccctgccgc cgggcggcac tgacgggcac gggctgcgac agaccgccgt
     2041 caacatatgt atgcctgccg atgataacgg gttggaacac attgggaatg gcggcggaag
     2101 cgcgcacagc ctgcccggca ttcccctgat tgaaagcgac ggccttgccg gtttcaaaat
     2161 cagtagcaat agcggcaaat ttgatgggaa actgctgaat ccgcctgccg ccgacttttc
     2221 ggttgatgta attttgcagc ttttcgcctt tgataaaacc actggtggac aaggttaaat
     2281 cgactaaatc cgttttacct aaaatctcgg cttccaattc aaggcggtcg ggcgacatac
     2341 ccgatgcaaa aaggctgccg actatcgaac ctgccgatgt gccggttact acttttacag
     2401 gaataccgtt ttctttcaaa accttaataa tgcctatatg ggcaaatcct ttagacgcgc
     2461 cgccaccgag tgccaaaccg accactgcgg cgggtttggc ggtttgcacc ggcttgcggg
     2521 cggcattgtt tcccgccgtg ccgcaggcgg caagcaacgc ggcggcggcg attgccaaaa
     2581 gcggtctgat ttttgaaaac gttaccatat tttccattcc tttatatatc gcaccccgtc
     2641 aaaaagaggg attgcttttc ttaacaaccc cctttgacag ccaagcaaat gggggctttg
     2701 ttaagtcatc atcaaaatta atatttcttt ttttccttta cggaaattat atttgaaggc
     2761 atactatcca aggcgggaat tatctcacaa caccgccgtt atccaaatat cccgcctttt
     2821 tcctttcctt tccatcaaaa tactttcttt ttatattcat taacttgtta aatcattggc
     2881 tgccgggtgt cagtttttcc gacaaaatcc gtctaatggg gtatcaacag aaccaaaaca
     2941 ggaacactta tgaaaatcgg aacaacttgg cagacggcat ccgctatgct ggttttgcgt
     3001 ctgtttgccg catatgaatt tttggaatcg ggtttgcaaa aatggaacgg ggagaattgg
     3061 ttttctgaaa tcaacgatca attcccattc ccgttcaact tgctgccgga cgcgttaaac
     3121 tggaatctcg ctatgtatgc ggagctttta ctgcccgtac tgttgctttt gggtttggca
     3181 acgcgtctgt cggcattagg gctgatggtc gttaccgccg tcgcttgggc tgcggttcac
     3241 gccggttcgg gttacaatgt ctgcgacaac ggttataaaa tggctttaat ttatatcgtg
     3301 gtattaatcc cgctgctttt ccagggtgcg ggcggatggt cgctggatac gctgctgaaa
     3361 aaacggtttt gcccccaatg ccgtctgaag caagattgat tcagtcgtgg aatctgactt
     3421 taaacattcc aaccttatct cgttaacttg atattctgaa aaggaaatag aaatgaacaa
     3481 aaacattgct gccgcactcg ccggtgcttt atccctgtct ttggccgccg gtgcagttgc
     3541 cgccgacaaa ccggcaagca acgcaacagg cgttcataaa tccgcccatg gctcttgcgg
     3601 cgcgtccaaa tctgccgaag gttcgtgcgg cgcggctggt tctaaagcag gcgaaggcaa
     3661 atgcggcgag ggcaaatgcg gtgcgaccgt aaaaaaaacc cacaaacaca ccaaagcatc
     3721 taaagccaag gccaaatctg ccgaaggcaa atgcggcgaa ggcaaatgcg gttctaaata
     3781 atcccacccc ttcaaaccaa gccgcgtttt tcagtaaaat gcggcttttt ttaacggcaa
     3841 acaaagattt tttaacaagc acatcattct tttgtgccat ccgaaccggg taaaaatatg
     3901 attcaacacg caggcttggg ctaccgccgc gacttggcgg aagactttct ctcgctttcc
     3961 gaaaacagcc cgatatgctt tatcgaagcc gcaccggaaa actggctgaa aatgggcggc
     4021 tgggcgcgca aacagtttga ccgtgtggcg gaacggctgc cgctggcgtt gcacggatta
     4081 tctatgtcgc tgggcgggca agcaccgctg gatactgatt tgatagacgg catcaaagaa
     4141 atgatgcgcc gttacgattg cacgtttttc tccgaccatt tgagctactg ccacgacggc
     4201 ggtcatcttt acgatttgtt gccgctgccc tttatcgagg aaatggtgca tcatacggcg
     4261 cggcgtatcc gcgaagtgca agaccgtttg ggctgccgca tcgccgtgga aaacacgtcc
     4321 tactatctgc attccccgct tgccgagatg aacgaggtcg agttcctcaa cgcggttgca
     4381 cgtgaggccg attgcggcat tcatctggac gtgaacaata tctacgtcaa cgccgtcaat
     4441 cacggcctgt tgtcgccgga ggcttttttg aaaaacgttg acgcagatcg cgtgtgctat
     4501 atccatattg ccggacatga cgtggaaacg ccggaattgt tgattgatac acatggcgcg
     4561 gcagttttgc cgactgtttg ggacttgctc gaacttgcct atgccaaact gccgacgatt
     4621 ccgcccaccc tgttggaacg cgattttaat ttcccgcctt ttgccgaact cgaagccgaa
     4681 gtcgccaaaa tcgccgatta tcaaacgcgt gccggaaagg aatgccgccg tgcagcctga
     4741 aacctccgcc caataccagc accgtttcgc ccaagccata cgcgggggcg aagccgcaga
     4801 cggtctgccg caagaccgac tgaacgtcta tatccgcctg atacgcaaca atatccacag
     4861 ctttatcgac cgctgctata ccgaaacgcg gcaatacttt gacagcgaag aatggagccg
     4921 tctgaaagaa ggtttcgtcc gcgacgcgca cgcccaaacg ccctattttc aagaaatccc
     4981 cggcgagttc ctacaatatt gccaaagcct gccgctttca gacggcattt tggcactgat
     5041 ggattttgaa tatacccaat tgctggcaga agttgctcaa attccggata ttcccgacat
     5101 tcattattca aatgacagca aatacacacc ctcccctgcg gcctttatcc ggcaatatcg
     5161 atatgatgtt accgatgatt tgcatgaagc ggaaacagcc ttgttaatat ggcgaaacgc
     5221 cgaagatgat gtgatgtacc aaacattgga cggcttcgat atgatgctgc tagaaataat
     5281 gggttcctcc gcgctttcgt ttgacaccct cgcccaaacc cttgtcgaat ttatgcctga
     5341 ggacgataat tggaaaaata ttttgcttgg gaaatggtca ggctggactg aacaaaggat
     5401 tatcatcccc tccttgtccg ccatatccga aaatatggaa ggcaattccc cgggccaaaa
     5461 ccatctatcc gcataaaatt accttgttcc cgatactatg ccgctacccg acctgaccga
     5521 tgccgaatta atagagtcgc gtaaactgct tctgcatttt gcgcggcttc agttgcccga
     5581 ccaccctgat ttggctgaag atttagtgca ggaaacattg ctgtccgcat acagcgcagg
     5641 cgacagtttt caaggcaggg cacttgtcaa cagctggctt tttgccatat tgaaaaacaa
     5701 aattattgac gcattacgtc aaatcggaag gcagaggaaa gtctttacca cactggatga
     5761 cgagctactg gatgaagcat ttgaaagcca tttttcccaa aacgggcatt ggacgccgga
     5821 agggcagccg caacattgga acactccgga aaaatcatta aacaacaacg aattccaaaa
     5881 aattctgcaa agctgcctat acaacctgcc tgaaaacacc gcacgggtat ttaccctgaa
     5941 ggaaatactc ggtttttcat ccgacgaaat acaacaaatg tgcggtatca gcacgtccaa
     6001 ctaccacacc attatgcacc gcgcccgaga atcattgcgc caatgcctgc aaatcaaatg
     6061 gttcaaccaa gaaaacccga agtaaacgtt atgaaaaaat gccgcgatat cgccctgctt
     6121 ctttccaaac atcaggaccg ggaaaccacc ccgggcgaga agatttccat atacacacac
     6181 ctgctgttct gtccgtattg ccgtgaatat aaaagacaac ttcaaaccat caaaagatca
     6241 ctggcaaaaa caaccagaac ttcaaaataa atgccgtctg aaaaggcttc agacggcata
     6301 agctgacgga aacaaatcaa accgatttac tgttatcggc agttcatcca taatacacgc
     6361 ttcaaaagca gcatattcct ccatacggaa tatataaata cgcaaaacac gaaggctgca
     6421 tcaatttgct ttatttgcct tatttcacag acggcgctac ccctcccgcc caacccgttc
     6481 tttctgaatg agcagatttc aatgattaag gaaaccctaa tgcgcccaat cttcctatct
     6541 ttcgttttac tccctatttt gataaccgcc tgcagcacac cggacaagtc tgcccgatgg
     6601 gaaaatatcg gcacaatctc aaacggcaat attcatacat atatcaataa agacagcgtg
     6661 agaaaaaacg gaaatctgat gattttccaa gataaaaaag ttgttaccaa tctaaaacaa
     6721 gaacgttttg ccaacacccc cgcatacaag actgccattg ccgagtggga aatccactgc
     6781 aacaacaaaa cataccgctt aagttcgcta cagttgtttg atacaaaaaa cacggaaatt
     6841 tccacacaaa actacacagc ctcttccctc cgcccgatga gcatcctgtc cgggacatta
     6901 accgaaaaac aatatgaaac cgtatgcgga aaaaaactct gattgcaact tatacacaaa
     6961 cttacccaca aaccttatca taaaaatgcc gtctgaaata ctgaaatatc agcatttcag
     7021 acggcatttt gccattccct gaaaattatc cacaaagtta tccacattat tttttaaaac
     7081 cggcttccat ccgaaatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
     7141 acagtacaaa tagtacggca aggcgaggca acgccgtact ggtttaaatt taatccacta
     7201 tataaactcg ctatacaatt tcactatcca aacgtaaatt gttccattga tacacaaaac
     7261 tgcttacccc cataattttg ataaagcatt tcttacattc ccggctccgt cccgtaacca
     7321 acacagcggc ggattcgcat ttgaagtgca actttcccta acagaaaaag gccagtatgc
     7381 ggtagcatac g
//
LOCUS       DAWHRA010000102         7279 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWHRA010000102 DAWHRA010000000
VERSION     DAWHRA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.102"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(139..223)
                     /locus_tag="WIB30_001600"
     tRNA            complement(139..223)
                     /locus_tag="WIB30_001600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(187..189),aa:Leu,seq:caa)
     gene            499..1152
                     /locus_tag="WIB30_001601"
     CDS             499..1152
                     /locus_tag="WIB30_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ3797642.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1231..1884
                     /locus_tag="WIB30_001602"
     CDS             1231..1884
                     /locus_tag="WIB30_001602"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ3797643.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1988..2887
                     /locus_tag="WIB30_001603"
     CDS             1988..2887
                     /locus_tag="WIB30_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ3797644.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLDEINSNELILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2884..4173
                     /gene="hisD"
                     /locus_tag="WIB30_001604"
     CDS             2884..4173
                     /gene="hisD"
                     /locus_tag="WIB30_001604"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ3797645.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4219..5316
                     /locus_tag="WIB30_001605"
     CDS             4219..5316
                     /locus_tag="WIB30_001605"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ3797646.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALRHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5313..6230
                     /gene="hisB"
                     /locus_tag="WIB30_001606"
     CDS             5313..6230
                     /gene="hisB"
                     /locus_tag="WIB30_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ3797647.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6325..7194
                     /locus_tag="WIB30_001607"
     CDS             6325..7194
                     /locus_tag="WIB30_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ3797648.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1903 a         2135 c         1738 g         1503 t
ORIGIN      
        1 tcagcacctt agagaatcgt tctctttgag ctaaggcgag gcaacgccgt actggtttaa
       61 atttaatcca ctatatttag tcttatctat ttcattaaac agcaatagac aaaaaaataa
      121 ccgctccaaa agcggttgtg gtgcccaggg tcggactcga accgacacac cttgcggcgg
      181 gggattttga gtcccctgcg tctaccaatt tcgccacctg ggctggtgaa gaagtcgtca
      241 ttataatggc ttttgaaatt ctgtaaacct ttttttgaaa ttattttatc tgtttttatt
      301 ttatttttga ttttaaatag aatttttatt attttaatct tactgttctt tccgttccaa
      361 agattctgta tgattcggca attcctgccg tgcagacaac gtaaaaaaat actacattaa
      421 atctgccaaa cgcgttaaga tggaaatatt caaattccgt acgaatcagg ttttgctatt
      481 tattcttggg agattgtcat gttttccgta ccgcgttcct ttttgccggg cgttttcgta
      541 cttgccgcgc ttgccgcctg caaacctcaa gacaacagtg cggcgcaagc cgcttcttca
      601 agtgcatccg cgtcggctgc ggaaaatgcg gcaaagccgc aaacgcgcgg tacggatatg
      661 cgtaaggaag acatcggcgg cgatttcacg ctgaccgacg gcgaaggcaa gcctttcagc
      721 ctgagcgatt tgaaaggcaa ggtcgtgatt ctgtctttcg gctttacgca ctgtcccgat
      781 gtctgcccga cagagctttt gacgtacagc gacacgttga agcagttggg cgggcaggct
      841 aaggacgtga aagtggtgtt cgtcagcatc gatccggaac gcgacacgcc tgaaatcatc
      901 ggcaagtatg ccaaacagtt caatccggac tttatcggtc tgacggcaac gggcggccaa
      961 aacctgccgg tcatcaagca gcaataccgc gtggtttctg ccaaagtcaa tcaaaaagac
     1021 gacagcgaaa actatttggt cgaccactct tccggtgcgt atctcatcga caaaaacggt
     1081 gaggttgcca ttttctcgcc ttacggaagc gagccggaaa cgattgctgc cgatgtaagg
     1141 accctgctct gataaaaccg tatgccgtct gcaccgtcgg cgcctgttca gacggcatta
     1201 ttgtttcaac cgacaaagga catccacacc atgcaggata atgctttgac catcgcctta
     1261 tccaaggggc gcatttttga ggagacgctg ccgctgcttg ccgctgccgg cattgttccg
     1321 actgaagagc ctgaaaaatc gcgcaagctg attatcggga cgaaccatga aaacatccgc
     1381 cttgtcattg tccgcgcaac cgatgtgccg acttatgtcc gctacggcgc ggcggacttc
     1441 ggcattgcgg gcaaagacgt actgatcgaa cacggcggca cggggcttta ccggcctttg
     1501 gatttggaga ttgccaagtg ccgcatgatg gttgctgtgc gtaaagggtt tgattacgaa
     1561 gcagcttcgc aacccggatg ccgtctgaag attgccacga agtatcctga aatcgcggca
     1621 tctcattttg ccggcaaggg tgtccatgtg gacattatca aactgtacgg ctcgatggaa
     1681 cttgcgccgc tggtcggctt gagcgatgcg attgtggact tggtttcgac gggcaacacc
     1741 ttgaaggcaa acggcttgga agcagtcgaa cacatcgtcg acatttccag ccgcctggtg
     1801 gtcaacaagg ctgctttgaa aacgaaatac gcgctgctgg agccgattat tcaggcgttc
     1861 ggcggcgcag tgaaggcgaa gtaagcattc atttgaataa agatgcgttt tcagacgacc
     1921 ctatccgttc ccgccgacag gtcgtctgaa aatatcaccg gcagtaaact gtataggaga
     1981 agttaaaatg gttgcaaaaa taaaaaaatt ctcagattca accctttccg ttttgaataa
     2041 cggcgagcgt cggttttatg tctattgtct gaccgacctg aaaaaagaca aaatcctcta
     2101 catcggcaaa ggctgcggta atcgtatctt cgagcatgaa tgggttgcta gtcgttcaca
     2161 agacccagtc tccggcgaga ttatcgatcg gaaactcaaa gccatctcca aatgcaagaa
     2221 actcggtcgc tatatcatca gctatcatct gactgaagtc gaagcgctcg ccgccgaatc
     2281 tgccttaatt cattttgtta aatctgtctt gggtaaaaaa ctcaaaaata aaattgccgg
     2341 gcatggtccg ggtggtatta gcgtagaaga actagatcgc cgctttggat tctcttctct
     2401 cccacttgat gagatcaact ccaacgagct aattctcgcc atcaaaatcc acaatgcttt
     2461 cgatttagat actgacgaag aattagacta ccttttcgac aaccaagacg atgccaacct
     2521 caaatcgcgt acgttgggca actgggttat cggtaaagat gttgcttcaa aagtgaaata
     2581 cgttatcggc gttcacaccg gtctgcaaaa cgctgttgtc agtgcatacg aagtggacgg
     2641 ttttgaaaca atggttgagg aaaccaaaaa cggtagaaaa caatcccgtt accgtttccg
     2701 cactacctct cgtagcgaag aggtattagc caaactcggt ctgcaacaaa aatgcctgcc
     2761 cgaattgaag tttggtagcg ggggagaaaa agcgtatatc agacccaaaa cagagacaga
     2821 aactgaacaa gagaatattc agacgacccc caatccaaaa ataaaaaagg aaaaaaccaa
     2881 atcatgaaaa aactcaacac ccaatcgccc gatttccaag ccggactcaa agccctgctg
     2941 gcttttgaaa ccgcgcaaaa ccccgaaacc gaacgcatcg tcgccgacat ttgcgccgac
     3001 gtgcaaaagc gcggcgatgc ggctttgatt gaatacacca acaaattcga tcagacaaac
     3061 gctaaaagca tcgatgattt aatactcacg caagccgatt tgaacgcggc gttcgagcgc
     3121 attccgaacg acgttcagac ggcattgcag accgccgccc gccgtgtcga aagctaccac
     3181 caacgccaaa aaatggaatc gtggagctac accgatgaag acggcacgct gttgggacaa
     3241 caaatcacac cgcttgaccg cgtcggcatt tacgtccccg gcggcaaggc ggcgtatccg
     3301 agttccgtca tcatgaacgc catgcccgcc cacgtcgcag gtgtgaaaga aatcatcatg
     3361 gtcgtgccga caccaaaagg cgaacgcaac gacatcgtac ttgccgccgc atacgtcgcc
     3421 ggcgtaacca aagtcttcac cgtcggcggc gcgcaggcgg ttgccgccct cgcctacggc
     3481 acggaaacca tcccccaagt cgataaaatc accggtccgg gcaacgcctt cgtcgccgcc
     3541 gccaaacgcc gcgtgttcgg cgtggtcggc atcgacatgg tggcggggcc gtctgaaatc
     3601 ctggtcatcg ccgacggcac gacacctgcc gattgggtgg cgatggattt gttcagccag
     3661 gccgaacacg acgaaattgc ccaagccatc ctcatcggca cgtcgcaagc gtatctcgac
     3721 gaagtagaag ccgctatgga ccgcctgatc gaaactatgc cgcgccgcga catcatcgaa
     3781 gcctcgctcg gcaacagggg cgcgatgata ctcgccaaag acttggacga agcctgcgaa
     3841 atcgccaact acatttcccc cgaacacttg gaactgtcag tcgaaaaccc gcaggaatgg
     3901 gcgaaaaaaa tccgccacgc cggtgcgatt ttcatgggac gctacaccgg cgaaagcctc
     3961 ggcgactact gcgccggtcc aaaccatgtg ttgcccacca gccgaaccgc ccgcttttcc
     4021 tcgcctttgg ggacatatga tttccaaaaa cgctccagcc tgattcaggt ttcggaacag
     4081 ggcgcgcaaa aattaggcga aaccgccagc gtgctggcac acggcgaaag cctgaccgcc
     4141 cacgcccgcg cggcagagtt ccgtatgaaa taatgccgaa acggcgtaca ggcatattcc
     4201 aaccattaag gaaacacgat gaaatccgtc cgctccttca tccgcgacga catacaagct
     4261 atgtcggcat atcagattgc cgacgttccg cccggctttg ccaaactcga ttcgatggaa
     4321 agtcccgtcc acccttttgc cggacatgaa acgctgttgc aggaatggca ggcacggctt
     4381 gccgccgcgc ccatccatct ttaccccaat ccctccggca gcggtttaca ggaagcatta
     4441 cgttcggcgt tcgacattcc cgactgcgcc gacatcgcgc tgggcaacgg ttcggacgaa
     4501 ctgatacagt tcatcacgat gctgaccgcc aaaccgggcg cggcaatgtt ggcagccgaa
     4561 cccagtttcg tcatgtaccg ccacaacgcc gcgctgtacg gcatggatta tgtcggcgtt
     4621 ccactgaacg gagatttcac cctcaacctg cccgccgtcc tcgaagccgt caggaaacac
     4681 cgccctgccc tgacctttat cgcctacccc aacaacccca ccggcgtatg cttcacgcgt
     4741 gccgaaatcg aagccgtcat cgaagcttca gacggcatcg tcgtcgtcga tgaagcctac
     4801 ggcgcattca acggcgacag cttcctgccg caggcaggca ggattcccaa cctgatagtc
     4861 ttacgcaccc tcagcaaaat cggttttgcc ggactgcgta tcggttatgc ggcaggctgc
     4921 cccgaagtca tcggcgaact gcaaaaaatc ctgccgccct acaatatgaa ccaattgagc
     4981 ctgaccactg ccaaactcgc cctgcggcac tacggcatta tctctgccaa catcgacagc
     5041 ctgaaaaacg aacgcgaacg gatgttcgcc gaattgggca aaatatgccg tctgaacacc
     5101 tttccaagtc aggcaaactt cattaccata cgcgtacccg atgccgattt gttgtttgac
     5161 acgctcaaac aaaaccgcat cttggttaaa aaactgcatg gcgcgcaccc gcttttggaa
     5221 cactgcctgc gcattaccgt aggcagcccc gcacaaaacg atgccgttct caacatcatt
     5281 cgccaacttt actgccaacc aacggatttc ctatgaattt gactaaaaca caacgccaac
     5341 tgcacaactt tctgaccctc gcacaagaag caggttcgct gtccaagctc gccaaactct
     5401 gcggctaccg tacccccgtc gcactctaca aactcaaaca acgccttgaa aagcaggcag
     5461 aagacccaga tgcacgcggc atccgtccca gcctgatggc aaaactcgaa aaacacaccg
     5521 gcaaacccaa aggctggctc gacagaaaac accgcgaacg cactgtcccc gaaaccgccg
     5581 cagaaagcac cggaactgcc gaaacccaaa ttgccgaaac cgcatctgct gccggctgcc
     5641 gcagcgttac cgtcaaccgc aatacctgcg aaacccaaat caccgtctcc atcaacctcg
     5701 acggcagcgg caaaagcagg ctggataccg gcgtaccctt cctcgaacac atgatcgatc
     5761 aaatcgcccg ccacggcatg attgacatcg acattagctg caaaggcgac ctgcacatcg
     5821 acgaccacca caccgccgaa gacatcggca tcacactcgg acaagcaatc cggcaggcac
     5881 tcggcgacaa aaaaggcatc cgccgttacg gacattccta cgtcccgctc gacgaagccc
     5941 tcagccgtgt cgtcatcgac ctttccggcc gccccggact cgtgtacaac atcgaattta
     6001 cccgcgcact aatcggacgt ttcgatgtcg atttgtttga agaatttttc cacggcatcg
     6061 tcaaccacag tatgatgacc ctgcacatcg acaacctcag cggcaaaaac gcccaccatc
     6121 aggcggaaac cgtattcaaa gccttcgggc gcgccctgcg tatggcagtc gaacacgacc
     6181 cgcgcatggc aggacagacc ccctcgacca aaggcacgct gaccgcataa aaaaccatac
     6241 cgtctgaaac acccgcaggc ttttcagacg gtatcggaac agataagatt acactacact
     6301 acaaacagaa aaggagtaaa catcatgtcc gcaaacgaat acgcacaaat cggctggata
     6361 ggcttagggc aaatgggtct gcctatggta acgcggctct tggacggcgg catcgaagtc
     6421 ggcgtataca accgctcgcc cgacaaaact gcccccatct ccgccaaagg cgcaaaagtt
     6481 tacggcaaca ccgccgaact cgtccgcgac tatcccgtca ttttcctgat ggtttccgac
     6541 tatgccgccg tgtgcgacat cctgaacgga gtccgcgacg gattggccgg caaaatcatc
     6601 gtcaacatga gcaccatctc cccgaccgaa aacctcgccg tcaaagcact tgtcgaagcc
     6661 gcaggcggac agtttgccga agcacccgtt tccggatcgg tcgggcccgc caccaacggc
     6721 acgctgctga ttctgttcgg cggcagcgaa gccgttttaa acccgctgca aaaaatattt
     6781 tccctcgtcg gcaaaaaaac cttccatttc ggcgatgtcg gcaaaggttc gggcgcgaaa
     6841 ctcgtcttga actcgctctt gggcattttc ggcgaagcgt acagcgaagc gatgctgatg
     6901 gcgcggcagt tcggcatcga taccgacacc atcgtcgaag ccatcggcgg ctcggcaatg
     6961 gactcgccca tgttccaaac caaaaaatcc ctgtgggcaa accgcgaatt cccgcccgcc
     7021 ttcgccctca aacacgcctc caaagacctc aacctcgccg tcaaagagct tgaacaggca
     7081 ggcaacaccc tgcccgccgt cgaaaccgtt gctgccagct accgcaaagc agtcgaagcc
     7141 ggctacggcg aacaggacgt ttccggcgtt tacctgaaac tggcagaaca ctgattgcct
     7201 tttccaaaca caatgccgtc tgaacatatt tcagacggca tttttatcac cccacgctta
     7261 aaatcagtcc cgattatga
//
LOCUS       DAWHRA010000103         7239 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWHRA010000103 DAWHRA010000000
VERSION     DAWHRA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.103"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WIB30_001608"
     CDS             complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WIB30_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ3797649.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RTATYADFTDTDGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1554..1709
                     /locus_tag="WIB30_001609"
     CDS             1554..1709
                     /locus_tag="WIB30_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797650.1"
                     /translation="MKKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1706..2137
                     /locus_tag="WIB30_001610"
     CDS             1706..2137
                     /locus_tag="WIB30_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ3797651.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARCFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISAILIL
                     LFAVFLVWSGYPATAASLAGGTVFALAGAFVIGRSRDQGKN"
     gene            2271..6644
                     /gene="app"
                     /locus_tag="WIB30_001611"
     CDS             2271..6644
                     /gene="app"
                     /locus_tag="WIB30_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ3797652.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFTYKIVKRNNYKAGTKGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFND
                     DNNGTGKINAKHEHNSLPNRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTKTDISGNVDLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     NLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFR
                     FMSELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFT
                     LQNEHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALI
                     AAGRDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPATT
                     AFPRARRARRDLPQLQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFA
                     EDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENT
                     FDDGIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGGKIRRRVLHYGIQ
                     ARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPA
                     QHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAA
                     AAKGPQLEAQHSAGIKLGYRW"
     gene            complement(6960..7121)
                     /locus_tag="WIB30_001612"
                     /pseudo
     CDS             complement(6960..7121)
                     /locus_tag="WIB30_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(7119..7239)
                     /locus_tag="WIB30_001613"
                     /pseudo
     CDS             complement(7119..7239)
                     /locus_tag="WIB30_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1925 a         2082 c         1750 g         1482 t
ORIGIN      
        1 aaacggatcg gattcccgtt tttatgggat aacggaatgt tcagccggac accaccacga
       61 tgctttcgat ccgtttattt tccgatacaa aacctcgaaa aaatcacgcc gagcaggtcg
      121 tccgccgtaa actcgcccgt gatttcgccg catgcgacct gcgccaagcg caagtgttcg
      181 gcaaacagct cgatttgatg gttgccgcac aatgccgcca gcgacaattc ttcctgcgct
      241 gctttgagtg cgttgacgtg ccgcgtccgc gccaaaaaca acccttcgct ttcgccctgc
      301 caaccggcct cgcgcaacaa cgtccgtttc agcgcgtcca agccgtcgcc ggttttcgcc
      361 gacaacgcga tgacggtttc cgcgcccgta ccgaacccgc ctgccgcgtg tgcgtgcaaa
      421 tcggatttgc tgtggatttc gatgcgtttc aactccggcg gcaacgcgtc caaaatcgcc
      481 cgtgtctttt cattcaaacc ctcgcgcgga tcgaccaaca ccagcgcgac atcggcttcg
      541 gatacggctt tgcggctgcg ttcgatgccg atacgctcga ccacgtcgtc cgtctcgcgc
      601 aaacctgccg tatcgacaat atgcaccggc acgccgtcaa tcaggatacg ttccctgacc
      661 gcgtcgcgcg tcgttccggc aatatcggta acaatcgcca cttcgtcgcc cgccaacgcg
      721 ttcagcaggc tggacttgcc cacattcggc gcgccgacca atacgacatt cagaccttcg
      781 cgcaaaatcg cgccctgctg cgcgttggca agcacatcat ccacggcgcg gcgcaagccg
      841 tccagtttgc cgcgtgcgtc tgccgcttcg agaaaatcaa tgtcttcctc gggaaaatct
      901 aacgtcgctt cgaccagcat ccgcaaggta atcaagtctt cgaccagacc gtgtatccgc
      961 cgcgaaaaat cgcccttgag cgagcgcaaa gccagacgcg ccgccgaacg gctggatgcg
     1021 tcaatcaaat ccgccacgcc ttccgcctgt gccaagtcca gtttgtcgtt caaaaacgca
     1081 cgcttcgtaa actcgcccgg ttcggcaagg cgcgcgccca attccaaaca gcggttcagc
     1141 agcatatcca tcaccaccgg cccgccgtgt ccctgaagct cgatgacatc ttcacccgta
     1201 aaacttgccg gtgcggcaaa aaacagcaaa agcccgctgt cgattgcctg tccgtccgtg
     1261 tccgtaaaat cagcataggt tgcggtacgc ggcttgggcg ttttcccgca caaagcctgc
     1321 gccatcggca gcaggttttt ccccgatatg cgtatcacgc ccacgccgcc gcgccctggt
     1381 gcggtagcga ctgccgcaat cgttggaacg ttatccgaca taaaaccccc gaaaattcaa
     1441 aacagccgcg attatagcaa atgccgtctg aagtccgacg gtttggcttt cagacggtat
     1501 aaaaccgcaa aaatgcttga taaatccgtc cgcctgacct aatatataac catatgaaaa
     1561 aacgaaacac atacgccctc ctgctcggta taggctcgct gctgggtctg ttccatcccg
     1621 caaaaaccgc catccgcccc aatcccgccg acgatctcaa aaacatcggc ggcgattttc
     1681 aacgcgccat agagaaagcg cgaaaatgac cgaaaacgca caggacaagg cgcggcaggc
     1741 tgtcgaaacc gtcgtcaaat ccccggagct tgtcgagcaa atcctgtccg acgagtacgt
     1801 gcaaataatg atagcccggt gtttccattc gggaccgttg ccgccgccgt ccgacttggc
     1861 gcaatacaac gacattatca gcaacggggc agaccgcatt atggcaatgg cggaaaaaga
     1921 acaagccgtc cggcacgaaa ccatacggca agaccaaacc ttcaacaggc gcgggcaact
     1981 gtacggcttc atcagcgcca tcctgatact gctttttgcc gtcttcctcg tatggagcgg
     2041 ctaccccgca accgccgcct cccttgccgg cggcacagtg tttgccttgg cgggtgcttt
     2101 cgtgattgga agaagccgag accaaggcaa aaattaatga caaatcctag ggcgtgcttc
     2161 atatccgccc gaacgccgaa ccgcacatat aggcacatcc cgcgcgccgc ccggaagcgg
     2221 aagccgcgcc ctcccaaaca aacccgaatc ccgtcagata aggaaaaata atgaaaacaa
     2281 ccgacaaacg gacaaccgaa acacaccgca aagccccgaa aaccggccgc atccgcttct
     2341 cgcctgctta cttagccata tgcctgtcgt tcggcattct tccccaagcc tgggcgggac
     2401 acacttattt cggcatcaac taccaatact atcgcgactt tgccgaaaat aaaggcaagt
     2461 ttgcagtcgg ggcgaaagat attgaggttt acaacaaaaa aggggagttg gtcggcaaat
     2521 caatgacaaa agccccgatg attgattttt ctgtggtgtc gcgtaacggc gtggcggcat
     2581 tggtgggcga tcaatatatt gtgagcgtgg cacataacgg cggctataac aacgttgatt
     2641 ttggtgcgga aggaagaaat cccgatcaac atcgttttac ttataaaatt gtgaaacgga
     2701 ataattataa agcagggact aaaggccatc cttatggcgg cgattatcat atgccgcgtt
     2761 tgcataaatt tgtcacagat gcagaacctg ttgaaatgac cagttatatg gatgggcgga
     2821 aatatatcga tcaaaataat taccctgacc gtgttcgtat tggggcaggc aggcaatatt
     2881 ggcgatctga tgaagatgag cccaataacc gcgaaagttc atatcatatt gcaagtgcgt
     2941 attcttggct cgttggtggc aatacctttg cacaaaatgg atcaggtggt ggcacagtca
     3001 acttaggtag tgaaaaaatt aaacatagcc catatggttt tttaccaaca ggaggctcat
     3061 ttggcgacag tggctcacca atgtttatct atgatgccca aaagcaaaag tggttaatta
     3121 atggggtatt gcaaacgggc aacccctata taggaaaaag caatggcttc cagctggttc
     3181 gtaaagattg gttctatgat gaaatctttg ctggagatac ccattcagta ttctacgaac
     3241 cacgtcaaaa tgggaaatac tcttttaacg acgataataa tggcacagga aaaatcaatg
     3301 ccaaacatga acacaattct ctgcctaata gattaaaaac acgaaccgtt caattgttta
     3361 atgtttcttt atccgagaca gcaagagaac ctgtttatca tgctgcaggt ggtgtcaaca
     3421 gttatcgacc cagactgaat aatggagaaa atatttcctt tattgacgaa ggaaaaggcg
     3481 aattgatact taccagcaac atcaatcaag gtgctggagg attatatttc caaggagatt
     3541 ttacggtctc gcctgaaaat aacgaaactt ggcaaggcgc gggcgttcat atcagtgaag
     3601 acagtaccgt tacttggaaa gtaaacggcg tggcaaacga ccgcctgtcc aaaatcggca
     3661 aaggcacgct gcacgttcaa gccaaagggg aaaaccaagg ctcgatcagc gtgggcgacg
     3721 gtacagtcat tttggatcag caggcagacg ataaaggcaa aaaacaagcc tttagtgaaa
     3781 tcggcttggt cagcggcagg ggtacggtgc aactgaatgc cgataatcag ttcaaccccg
     3841 acaaactcta tttcggcttt cgcggcggac gtttggattt aaacgggcat tcgctttcgt
     3901 tccaccgtat tcaaaatacc gatgaagggg cgatgattgt caaccacaat caagacaaag
     3961 aatccaccgt taccattaca ggcaataaag atattgctac aaccggcaat aacaacagct
     4021 tggatagcaa aaaagaaatt gcctacaacg gttggtttgg cgagaaagat acgaccaaaa
     4081 cgaacgggcg gctcaacctt gtttaccagc ccgccgcaga agaccgcacc ctgctgcttt
     4141 ccggcggaac aaatttaaac ggcaacatca cgcaaacaaa cggcaaactg tttttcagcg
     4201 gcagaccaac accgcacgcc tacaatcatt taaacgacca ttggtcgcaa aaagagggca
     4261 ttcctcgcgg ggaaatcgtg tgggacaacg actggatcaa ccgcacattt aaagcggaaa
     4321 acttccaaat taaaggcgga caggcggtgg tttcccgcaa tgttgccaaa gtgaaaggcg
     4381 attggcattt gagcaatcac gcccaagcag tttttggtgt cgcaccgcat caaagccaca
     4441 caatctgtac acgttcggac tggacgggtc tgacaaattg tgtcgaaaaa accattaccg
     4501 acgataaagt gattgcttca ttgactaaga ccgacatcag cggcaatgtc gatcttgccg
     4561 atcacgctca tttaaatctc acagggcttg ccacactcaa cggcaatctt agtgcaaatg
     4621 gcgatacacg ttatacagtc agccacaacg ccacccaaaa cggcaacctt agcctcgtgg
     4681 gcaatgccca agcaacattt aatcaagcca cattaaacgg caacacatcg gcttcgggca
     4741 atgcttcatt taatctaagc gaccacgccg tacaaaacgg cagtctgacg ctttccggca
     4801 acgctaaggc aaacgtaagc cattccgcac tcaacggtaa tgtctcccta gccgataagg
     4861 cagtattcca ttttgaaagc agccgcttta ccggacaaat cagcggcggc aaggatacgg
     4921 cattacactt aaaagacagc gaatggacgc tgccgtcagg cacggaatta ggcaatttaa
     4981 accttgacaa cgccaccatt acactcaatt ccgcctatcg ccacgatgcg gcaggggcgc
     5041 aaaccggcag tgcgacagat gcgccgcgcc gccgttcgcg ccgttcgcgc cgttccctat
     5101 tatccgttac accgccaact tcggtagaat cccgtttcaa cacgctgacg gtaaacggca
     5161 aattgaacgg tcagggaaca ttccgcttta tgtcggaact cttcggctac cgcagcgaca
     5221 aattgaagct ggcggaaagt tccgaaggca cttacacctt ggcggtcaac aataccggca
     5281 acgaacctgc aagcctcgaa caattgacgg tagtggaagg aaaagacaac aaaccgctgt
     5341 ccgaaaacct taatttcacc ctgcaaaacg aacacgtcga tgccggcgcg tggcgttacc
     5401 aactcatccg caaagacggc gagttccgcc tgcataatcc ggtcaaagaa caagagcttt
     5461 ccgacaaact cggcaaggca gaagccaaaa aacaggcgga aaaagacaac gcgcaaagcc
     5521 ttgacgcgct gattgcggcc gggcgcgatg ccgtcgaaaa gacagaaagc gttgccgaac
     5581 cggcccggca ggcaggcggg gaaaatgtcg gcattatgca ggcggaggaa gagaaaaaac
     5641 gggtgcaggc ggataaagac accgccttgg cgaaacagcg cgaagcggaa acccggccgg
     5701 ctaccaccgc cttcccccgc gcccgccgcg cccgccggga tttgccgcaa ctgcaacccc
     5761 aaccgcagcc ccaaccgcag cgcgacctga tcagccgtta tgccaatagc ggtttgagtg
     5821 aattttccgc cacgctcaac agcgttttcg ccgtacagga cgaattagac cgcgtatttg
     5881 ccgaagaccg ccgcaacgcc gtttggacaa gcggcatccg ggacaccaaa cactaccgtt
     5941 cgcaagattt ccgcgcctac cgccaacaaa ccgacctgcg ccaaatcggt atgcagaaaa
     6001 acctcggcag cgggcgcgtc ggcatcctgt tttcgcacaa ccggaccgaa aacaccttcg
     6061 acgacggcat cggcaactcg gcacggcttg cccacggcgc cgttttcggg caatacggca
     6121 tcgacaggtt ctacatcggc atcagcgcgg gcgcgggttt tagcagcggc agcctttcag
     6181 acggcatcgg aggcaaaatc cgccgccgcg tgctgcatta cggcattcag gcacgatacc
     6241 gcgccggttt cggcggattc ggcatcgaac cgcacatcgg cgcaacgcgc tatttcgtcc
     6301 aaaaagcgga ttaccgctac gaaaacgtca atatcgccac ccccggcctt gcattcaacc
     6361 gctaccgcgc gggcattaag gcagattatt cattcaaacc ggcgcaacac atttccatca
     6421 cgccttattt gagcctgtcc tataccgatg ccgcttcggg caaagtccga acacgcgtca
     6481 ataccgccgt attggctcag gatttcggca aaacccgcag tgcggaatgg ggcgtaaacg
     6541 ccgaaatcaa aggtttcacg ctgtccctcc acgctgccgc cgccaaaggc ccgcaactgg
     6601 aagcgcaaca cagcgcgggc atcaaattag gctaccgctg gtaaccgccg gatatgccga
     6661 aaggggtctg acgatgccac cgtgcgctgt caaacccttt ttctgccgcc gccttgtgcc
     6721 tgctccattg tctgataacc gtcaaattga tttttagccc atgtttggtg ggtcggggaa
     6781 atctatatct tcgtccgtgc cgaaatagtc tgagaccttt gcaaaattcc tttccctccc
     6841 gacagccgaa acccaaacac aggttttcgt ctattttcgc cccaaatacc tcctaattct
     6901 acccaaatac ccccttaatc ctccacggac acccgataat caggcatccg ggctgctttt
     6961 taggcggcag cgggcgcact tagcctgttg gccgctttca aaaggttcaa acacatcgcc
     7021 ttcagatggc tttgcgcact cactttaatc agtccgaaat aggctgcccg agcgtagcgg
     7081 aatttacggt gcagcgtacc gaagctctgt tcgaccacat atagtggatt aaatttaaac
     7141 cagtacggcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgtcgccttg
     7201 tcctgattta aatttaatcc actataacgg gtcttcgac
//
LOCUS       DAWHRA010000104         7169 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWHRA010000104 DAWHRA010000000
VERSION     DAWHRA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.104"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(86..1489)
                     /gene="mtrE"
                     /locus_tag="WIB30_001614"
     CDS             complement(86..1489)
                     /gene="mtrE"
                     /locus_tag="WIB30_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ3797653.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAALNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1544..4747)
                     /gene="mtrD"
                     /locus_tag="WIB30_001615"
     CDS             complement(1544..4747)
                     /gene="mtrD"
                     /locus_tag="WIB30_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ3797654.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4759..5997)
                     /gene="mtrC"
                     /locus_tag="WIB30_001616"
     CDS             complement(4759..5997)
                     /gene="mtrC"
                     /locus_tag="WIB30_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ3797655.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAEQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPVVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIASITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
     gene            6401..7033
                     /gene="mtrR"
                     /locus_tag="WIB30_001617"
     CDS             6401..7033
                     /gene="mtrR"
                     /locus_tag="WIB30_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ3797656.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT         1682 a         1995 c         1868 g         1624 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataaaga aagaaatgcc gtctgaaacc ttatcgtttc
       61 agacggcatt ttattgcccg accggttatt tgtcggtttg ggtatcccgt ttcaatccgc
      121 cgccgagtgc cttgtacaaa tcggcaaggt tttcggcgcg ggtcagttgt gccgacaaag
      181 ccgcaccctc cgccgcatag ctgctgcgtt ccgcatcgag caaatcgagc gcgccggata
      241 cgccgtgctt gtaacgcagg ccgaccaagc gcaacgcttc tttagaggcg cggctttgtt
      301 tgcttaaagc gtcataggct ttatccagct gctcgcgcgc cgccaatgcg tttgccacgt
      361 cttgaaatgc ggattggacg gcggattcat aggcaacgat ttgtgcctgt tggcgcagct
      421 tggctacatc aaggttcgcc ttgttcgtac cccaggtaaa aatcggcagg gtaatagacg
      481 gcgcgaacga ccaaacgccc gtgccgcttt tgaacaaccc acccaattcg gcagaacccg
      541 taccgacggt tccggtcagg cggatggatg ggaaaaaggc ggcgcgtgcc gcaccgatat
      601 tggcgtttgc ctgtttgagc gcgtgttcgg cagcacggat atcgggacgg tcgagcaata
      661 cttcggaact caaaccggcc ggcagttttt caacaaaaaa ctgcttgtcc agcggcaaac
      721 cggcaggcag gtcttcgggt atcggttggt taatcaaggt tgccaaggca ttgcgcgcct
      781 gttcgcggct gcgcgcggca tgggcataat cggctttggc agattcgatc agggcttcct
      841 gctgacgtag ggcgacggcg gaaatcacgc ctgccttgta acgtaattcg gacagcttgt
      901 aggtttcctc gcgcgttttc aaaacacgtt gcgccaaaga catcgcttct tcggcgtaac
      961 gttcgttgaa ataggctttg gcaacggtgg caatcaggct caaatgtgcc gcatcgcggt
     1021 tggcggtgct ggcgaaatag ccttgcagtg ccgcctcgct gctgctgcgt acacgcccga
     1081 acagatcgag ttcgtaagat gccgcaccca gtccgacttt gtagctgctg cttacattgc
     1141 cgccgctcaa gctgccttgg cgcgagtcgt tcgcattggc ggcaagcgtg ggcaggaggt
     1201 tgttgcgctc aatcatgtat tgtttgcggt agatttcgct gttcaatgcg gcggtacgca
     1261 aactggtatt gcgctcgagt gcgatgtcga tcagcttttg caggcgcggg tcggcaaaat
     1321 agtcatgcca acctaaatcg accgcacgga tgccgctgtc ggcggtatcg tttttgaacg
     1381 tttcggcaac ttcgactttg ggctgctcgt attggggaat catggtgcag gcggataatg
     1441 cgaaggctgc tgcaacagaa gtcaaggtgg ttttcaatgt agtatccata aaaaagtcct
     1501 gatgccgtct gaaaacccgt gggcgttcag acggcatggt tgcttaatgt tggctgtcgt
     1561 ccgaaccggt gatgcccgct tcggcggcgt gttttactgc catttcgtgt tcgtgcgcgg
     1621 tttctttgaa gaatttgcgc accaccacat agaaaagcgg aacaaggaac acggacaaga
     1681 gcgtgccgat gagcatcccc cagaatacgg tcgtgccgat ggcgcgctgg ctggcagaac
     1741 ttgcaccacc ggcaatatac aggggaacca cgcccaaaat aaaggcgaac gaggtcatga
     1801 taatcggacg gaaacgcagg cgggcggctt ccaaagcggc ttcgaccgcg cttttccctt
     1861 gcgcttgaag gtctttggca aactcgataa tcaaaatcgc gtttttcgca ctcaaaccca
     1921 tcacggtaac gaaaccgact tgaaagtaga tgtcgttggc gaacgaggga acgctgccca
     1981 acagtccttc aaacaggttg cgcccggtta cgcccgcagc cgcaccgatc aaacccaacg
     2041 gaatcacaag gatgaccgcc agcggaatcg accagctttc ataaagcgcg gcaagtacca
     2101 aaaatacggc tgcaaccgcc aaaccgtaca aaatcagggt ttgcgagccg ccttttgcct
     2161 cttcgcgcga ctgtccgccc cattcgaagc tgtaaccgcc gcccaattcg tcaaccattt
     2221 tttgaaccgc cgccatagcc tgcccggtgg aaacgccggt tgcaggcgaa gcggacagct
     2281 tcatcgaagg ataaccgttg aagcgtacgc tctgttccgt accgttttcc caagaaacag
     2341 tagcaatggt ggaaagcggt acggcgacgc cggatttgtt cggcacggtc aggttcaaaa
     2401 tatcggcagg ctgcatacgg gcatcctcgt cggcctgcac catcacgcgt tgcagacggc
     2461 cttggttcgg gaagtcgctg acataagacg aactcagcgc acttgccaat gcggtgcgga
     2521 tgtcggcaaa cgaaatgcct tgcgccgccg cggcggcacg gttgatgtcg attttcaact
     2581 gcggcgagtc ttccaaaccg ccagcacgga cggtgctggg gtcaaacaaa ccgctggcac
     2641 gcattttctg aatcaactcg ttgcgcttcg ccagcaatgc ggtatggccg gtattattgc
     2701 ggtcttgcag gttgatgctc aggcccgaac cattgcccaa ctccagaatc ggaggcggga
     2761 cgacggcgat gccaaaaccg tctttaagcg tccccatcat catacccgtc agcttgccgg
     2821 caatcgcaac ggcatcgctg ccgggcgcgg tacgctcgtt ccaatctttc aatatggcaa
     2881 aacccatcgc catattctga ccgctgcccg aaaagctgaa gccggaaacg gtaatgatgt
     2941 tttctatttc aggaatgctt ttcgccagtt gggtaacttg cgccaaagtc gcattggtgc
     3001 gctcttgggt cgctcctgca ggcagttgca cgctgaccat gacgaagcct tggtcttcgg
     3061 tcggcaggaa tgaagtcggc aggcgcataa acaggaacac gcccacaacc gccaagccga
     3121 tatagacaac catcatgcgg aaagtcttac gcagcacttt ggcaacccgg ccttcgtaac
     3181 cgtgcgtcca actgttgaat ttcttgttaa accagccgaa gaaacctttt ttctcttcgt
     3241 gatgcccttt cgggattgtc ttcaacattg tggcacacaa agcaggggta agggtcagcg
     3301 caaggaaggc ggagaatgcg attgatgacg ccatcgtcag ggcaaactgt ttgtaaatat
     3361 tgcccgtcgc cccgctgaac atcgccaacg gtacgaacac ggaaatcaga acggcggtaa
     3421 taccgatgac cgcgcccgaa atctgaccca tcgctttttt ggtcgcttct ttgggcggca
     3481 agccttcacc cgccataatg cgctcgacgt tttcaaccac cacaatcgcg tcatcgacca
     3541 cgatgccgat gaccaaaacc atcgcaaaca tggtcagtac gttaatcgac atgcccatat
     3601 aagagatgaa ggcgaaaccg cccaacagcg aaatcggtac gacgatggtc ggaatcagcg
     3661 tataacggat gttttgcagg aagagataca ttacgacaaa caccagcacc atcgcttcga
     3721 ttaaagtgtg aatcactttt tcaatcgaaa tttcgacgaa tttggaagta tcgtaagggg
     3781 ttttccagct cataccctga ggaaagtatt tttccaacgt cgccatgcgt tctttaaccg
     3841 cctttgccgt cgccatcgca ttgccgctgt tggacagcat caccgccata ccggtggtat
     3901 ttacaccgtt cagacgggtt gaggaagaat agtcttccat acccagtccg acccttgcca
     3961 catccttcag gtaaacatta gaaccgtcgg tattggcgcg gaggatgacg ttgccgaatt
     4021 cttctgccgt acccaactgc ccttgcgccg ttacggtagc cgtaaccgtc tgtccgcgaa
     4081 cggcgggaag cgaaccgata gaacccgctg aaatctggac gttctgggcg gacagcgcgc
     4141 tgccgatatc ggcaaacgac aaattgtagt tttgcagttt cttaggatca acccaaatcc
     4201 gcatcgcgcg ttgcgcgccg aacaggcgta cctgccccac gccttcgata cgctgcaact
     4261 cgggaacgat attacgctgc gcgtagtcgt tcatctcttc ggttgactgc acatccgacg
     4321 aaagcatcac aatcatcagg aaattggaac gcgccttgga tacggttacg ccgtattgct
     4381 ggacggttgc cggcagcgtg ctcaatactt cggaaagctt gttctgcact tccacctgcg
     4441 ccagattctc gtcggtatcg ggcgtaaagg tcaggctcac gctgccgctg ccgctcgaat
     4501 cggcggaagt ggacatataa tccaaacctt ccacgccgtt catattccgc tcgatcacgg
     4561 aaagcacgct gtcttccatt acctgcgcgg acgcacccgg ataagtggcg tgcaaagtaa
     4621 tggtcggggc ggcgacagac ggatattgcg aaacaggcag gcttttgatg ccgaaaatac
     4681 ccgccgcaat aatgaaaatc gaaataaccc acgcaaaaat agggcggtcg ataaaaaatt
     4741 tagccatcga tgccttcctt attccgcttc agaagcagtt ttggcttcag atgccgtctg
     4801 aacgccggat tgaggcgcgg cggcttggtt ttcagacggc gcccattctt tgggcgttac
     4861 ctttttcgca cccgttatac tggcgatact gatgccttcc acaaccacct tgtccccgtc
     4921 cttcagaccc gacgtaacaa tccaattcgt accctgctgt tgcgcaaccg ttacctcgcg
     4981 gggttccata ccgccttggg cattcacaat catcacggta tctttcgcac cgcgcgttac
     5041 cgcctgctgc ggcacaacaa atgcgttatc caccgccact tggtccatca gcacgcgcac
     5101 atacagaccg ggcatcagga tattctgatc gttcggtacg gcggcgcgca gggtaatctg
     5161 accggtcgat tcgttgacga ccggatcggc aaacagcagg cggccttttt cagggtaaac
     5221 tgtgccgtcg tcaaatttga tgccgaccgc aatcacacca tccgccgcca gcagtttgcc
     5281 ttcggctatc tgacggcgca atttcatcac ttcggatgca gactgggtaa cgttcacata
     5341 catcggattg gtttggcgga tggttgccag cacggtcgta tcgcccgcat tcaacagcgt
     5401 accttcggaa actttagact gaccgataaa gccggaaatc ggcgcggtaa tgcgcgaacg
     5461 gttcagattg atgccggcag atttgatcgc cgcctgtgct gctttgacac ctgcctcggc
     5521 agaacgtttc gccgttaccg cagcatcgta ttcctgccgg ctgacggctt cggcggcaac
     5581 caaaggcttg tatcgcgcca aatccgcatc cgctttggca agcgttgcct gagccgttgc
     5641 cagttgcgcg cgcgcgcttt ccagacctgc ttcataagtg gaactgtcga tctgatacag
     5701 cggctgttcg gcacggacat aactgccttc ttggaacagg cgtttttgga tgatgccgcc
     5761 gacttgggcg cggacatcgg cggtacgcag cgattccaaa cgccccggca actcgacggt
     5821 caacgcgacg gtttgcggat ggacggttac gacaccgacg acgggcgcag gggcttcccg
     5881 accagcaggc tgcccgccct gcgccgcgtc tccgccttta ccgcaagacg acagtaccaa
     5941 tgcaacggcg gcagccaacg cggccgcacg catcgcctta aaagcataaa aagccattat
     6001 ttatcctatc tgtctggttt gatgtaaagg gttttgccaa tcaacaggca ttcttatagt
     6061 ggattaacaa aaaccagtac ggcgttgcct cgccttagct caaagagaac gattctctaa
     6121 ggtgctgaag cactaagtga atcggttccg tactatttgt actgtctgcg gcttcgtcgc
     6181 cttgtcctga tttttgttaa tccactatat ttcaggatat aaaaaccgcc tgctttgata
     6241 cccgaatgtt cgaacgggtt gcaaagcagg ttatgcctgt tttcaaagtt gagatgtagt
     6301 ctcaatttca tgggtttcat tatacataca cgattgcatg gttacaaagt cttttttata
     6361 atccgccctc atcaaaccga cccgaaacga aaacgccatt atgagaaaaa ccaaaaccga
     6421 agccttgaaa accaaagaac acctgatgct tgccgccttg gaaacctttt accgcaaagg
     6481 gattgcgcgc acctcgctca acgaaatcgc ccaagccgcc ggcgtaacgc gcggcgcgct
     6541 ctattggcat ttcaaaaata aggaagactt gttcgacgcg ctgttccaac gtatctgcga
     6601 cgacatcgaa aactgcatcg cgcaagatgc cgaagatgcc gaaggagggt cttgggcggt
     6661 attccgccac acgctgctgc actttttcga gcggctgcaa agcaacgaca tctactacaa
     6721 attccacaac atcctgtttt taaaatgcga acacacggag caaaacgccg ccgttatcgc
     6781 cattgcccgc aagcatcagg caatctggcg cgagaaaatt accgccgttt tgaccgaagc
     6841 ggtggaaaat caggatttgg ctgacgattt ggacaaggaa acagcggtca tcttcatcaa
     6901 atcaaccttg gacgggctga tttggcggtg gttctcttcc tgcgaacgtt tcgatttggg
     6961 caaaaccgcc ccgcgcatca tcgggataat gatggacaac ttggaaaacc atcccgacct
     7021 gcgccggaaa taatcaagcc ttggtaacaa tgccgtctga aacgaacaaa ccctttcaga
     7081 cggcatcaaa atgacacaaa gccttcttct aaaaatacat attgagacct ttgcaataac
     7141 ataggttact aaaattttat gctcaatct
//
LOCUS       DAWHRA010000105         7124 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWHRA010000105 DAWHRA010000000
VERSION     DAWHRA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.105"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            169..1092
                     /locus_tag="WIB30_001618"
     CDS             169..1092
                     /locus_tag="WIB30_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3797657.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAKPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="WIB30_001619"
     CDS             complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="WIB30_001619"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ3797658.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYGNAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(2693..4672)
                     /gene="tkt"
                     /locus_tag="WIB30_001620"
     CDS             complement(2693..4672)
                     /gene="tkt"
                     /locus_tag="WIB30_001620"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ3797659.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGIKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            5199..6695
                     /gene="ccoG"
                     /locus_tag="WIB30_001621"
     CDS             5199..6695
                     /gene="ccoG"
                     /locus_tag="WIB30_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ3797660.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            6698..>7124
                     /locus_tag="WIB30_001622"
     CDS             6698..>7124
                     /locus_tag="WIB30_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ3797661.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAIRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHW"
BASE COUNT         1497 a         1737 c         2130 g         1760 t
ORIGIN      
        1 aaaattcagg cggtgttttc aaatagcgcg aagcaatagc cgagcttcag ccggtttgtt
       61 tgaaatcaaa gggttggaat caaacgccaa acgcttgaac catcattgcc cgatgcggca
      121 aaccggcatc gtccggcaat acggttgttt aaaaatagga aaatcaggat ggaaaaccaa
      181 aggccgctcc taggcttcgc gttggcactt ttggcggcga tgacgtgggg aacgctgccg
      241 attgccgtgc ggcaggtatt gaagtttgtc gatgcgccga cgctggtgtg ggtgcgtttt
      301 accgtggcgg cggcggtatt gtttgttttg ctggcactgg gcgggcggct gccgaagcgg
      361 cgggattttt cttggtgctc attcaggctg ctgctgctcg gcgtggcggg catttcggca
      421 aactttgtgc tgattgccca agggctgcat tatatttcgc cgaccacgac gcaggttttg
      481 tggcagattt cgccgtttac gatgattgtt gtcggtgtgt tggtgtttaa agaccggatg
      541 actgccgctc agaaaatcgg cttggttttg ctgcttgccg gtttgcttat gttttttaac
      601 gataaattcg gcgagttgtc gggtttgggc gcgtatgcga agggcgtgtt gctgtgtgcg
      661 gcaggcagta tggcatgggt gtgttatgcc gtggcgcaaa agctgctgtc ggcgcaattc
      721 ggaccgcaac agattctgct gttgatttat gcggcaagtg ccgccgtgtt cctgccgttt
      781 gccaaaccgg cacacatcgg aagtttggac ggtacgttgg cgtgggtttg ttttgcgtat
      841 tgctgcttga atacgttaat cggttacggc tcgttcggcg aggcgttgaa acattgggag
      901 gcttccaaag tcagcgcggt aacaaccttg ctccccgtgt ttaccgtaat attttctttg
      961 ctcgggcatt atgtgatgcc tgatactttt gccgcgccgg atatgaacgg tttgggttat
     1021 gccggcgcac tggtcgtggt cgggggtgcg gttacggcgg cggtggggga caggctgttc
     1081 aaacgccgct agttcgcagg caacggaaaa tgccgtccga acgaggtttc agacggcatt
     1141 ttatttgagg gaaagattag cgcggatgaa ccatatcggc aggaacgacc agttcgtcaa
     1201 attcttcgcc cgtcagcaag cccaactcaa cggcggtttc gcgcaacgat ttgttgtttt
     1261 tgtaggcggt tttggcgact ttggcggcgt ttccgtaacc gattttacgg tttaacgcgg
     1321 taacgagcat cagggaatgg tgcaggaaat agtcgatttt ttccggtacg ggttcgatgc
     1381 cgatggcgca gtgttcgttg aagctgttgc acgcgtcgcc caagaggcgg atggattgca
     1441 agaggttgta ggcgataacg ggcatataga cgttcagctc gaaattgccc gacgcgcccg
     1501 ccatgccgat ggtaacgtcg ttgccgaaca cttggcagca caccatggtc atcgcttcgc
     1561 attgggtcgg gttgactttg cccggcatga tggacgaacc cggctcgttt tcggggattt
     1621 tgatttcgcc caaaccgcag cgcgggccgc ttgccagcca gcggatgtcg ttggcgattt
     1681 tattcaggct tgccgccagc gttttcaatg cgcccgaagc ggcaacggcg gcatcgcgtc
     1741 cgcccaaggc ttcaaatttg ttcggcgcgc tgacaaacgg caagccggac aattcggcga
     1801 gtttggcggc ggctttttcg gcgtattcgg gatggctgtt caaacccgtg ccgaccgccg
     1861 taccgcccaa agcaagttca tacaagtctt taagcgcgtc gttcagacgg cctaagccgt
     1921 ggtcgagctg ggaaacgtag ccggaaaatt cttggcctaa agtcagcggc gtcgcgtcct
     1981 gcaagtgggt gcggccgatt ttgacgatag gggcgaaagc ttgggctttt ttgtccaagg
     2041 tatcgcgcag ggcttttacg gcggggatga ggtggcggtt gatttcaatc gcggcggcaa
     2101 cgtggatagc ggtcgggaat gcgtcgttgg tcgattgcgc gtggttcaca tggtcgttgg
     2161 gatggacggg ctgataagcc gccaaacccg taccggcgat ttcgttggcg cggtttgcca
     2221 gcacttcgtt catgttcata ttggactgcg tgccggagcc ggtctgccaa accactaaag
     2281 gaaactgtcc gtcaagcctg ccgttcaaca catcatccgc cgcctgcgta atcaaatccg
     2341 cctgttccgg cttaatcctg ccgagggaaa cattggtcgc ggcggcggct tttttcacca
     2401 gcgccaaagc ataaatcagc ggctgcggca gggtttcgcc gccgattttg aaattgttgc
     2461 ggctgcgctg ggtctgcgcg ccccaatagg cttcggacgg gacttcgaca ttgcccatgg
     2521 tgtcgtgttc ggtacgggtg ctcatgcgtt tctccttttg aaatgtgaat aagagtgatt
     2581 cgcaaatatt ataatggaga ttggaggaat gaggaagtat caaggagaga gggcaaacaa
     2641 atgccgtctg aagccctaat tggtttcaga cggcatttgt ttaggtgtgg ggttacagca
     2701 cggatttcac cgtatcaacc acattgtcca cggtaaagcc gaatgctttg aagagtaaat
     2761 cggcaggggc ggattcgccg aagcggttga tgccgacgac tgcgccgttc agtccgacat
     2821 atttgtacca gccgtcggcg tggccggctt ctacggcgat gcgcggcagg ctttcgggca
     2881 ggacggcggc ttgataggcg gcgtcttggc ggtcgaatac gttggtggac ggcatggaaa
     2941 cgacgcgcac ggcgatgttt tgcgcggcga gggctttttg cgcttccaaa gccagctcga
     3001 cttctgagcc ggtggcaatg atgacggctt gggcgttgcc ttgggcttcg ctgatgatgt
     3061 agccgccgcg tttgatgtcg tccagttgtt gttcgctgcg cgcttggaat ttcaggtttt
     3121 gacggctgaa aatcaggcag gacgggtgat cggcggcttt gacggcttct gcccaagcga
     3181 ccaaggattc ggcggtgtcg cacggccgcc atacgtccat attcggaatc aggcgcaggg
     3241 tggcggtttg ctcgatgggt tggtgggtcg gaccgtcttc gcccaaaccg atggaatcgt
     3301 gggtgaacac aaatacaggg ttgattttca tcagtgccgc catacgcagg gcgttgcgct
     3361 cgtattcgct gaacatcagg aaggtcgcac cgaatggttt gatgccgccg tgcagggcaa
     3421 gaccgttcat aatcgcaccc ataccgaact cgcgcacgcc gtagtggatg tagttgccgc
     3481 ctttgtcgcg ggtaacggag acgctgtttg accagtcggt caggttggac ggggtcaggt
     3541 cggccgaacc gcccaccaat tcaggcaact cttttgccaa gatttcgatg ctgttttggc
     3601 tggctttgcg ggtagcgatg gtttcggctt tggcgcacac ttctttcaat gccgtctgaa
     3661 cgtattcatc gaaattgtcc ggcagctttt tatccatacg gcgcacaaat tctgcggctt
     3721 cggcaggata tttggcttga tattgcgcga acagttcgtt ccagtcggct tccagtttcg
     3781 cgccttgttc tttggcattc cacgcatcgt aaatttcttg cgggatttca aaggcggggt
     3841 aagtccagcc caaatgtttg cgcgtggctt cgatttcgtc cgcgcccaaa ggtgcgccgt
     3901 gggttttgtg gctgccttct ttgttggcac tgcctttgcc gattaaggtt ttgcagcaga
     3961 tgatggacgg tttgccggtt tcggcacgtg cggcttcgat ggcggcttga atggcggcgg
     4021 tgtcatgacc gtttacgtta ggaacgacgt gccagccgta gctttcaaag cgttgcggga
     4081 tgttttcggt aaaccagccg tccactttac catcaataga aatattgtta tcgtcataca
     4141 aaacaatcag tttgcccaag cctaaagtgc cggcgagcga acaggcttcg tgcgatacgc
     4201 cttccatcag acagccgtcg cccatgaaga cgtaggtgta atgatcgacg atgttcaaac
     4261 cgtctttatt aaattcggcg gcaaggattt tttctgccaa tgccataccc accgcgttgg
     4321 caatcccttg ccccaacggg ccggtcgtgg tttccacgcc gtcggtgtag ccgtattcgg
     4381 ggtggccggg ggttttgctg tgcagctggc ggaagttttt caagtcttca atgctcagat
     4441 tgtagccggt caggtgcagc aggctgtaca acagcataga cgcgtggccg ttggagagga
     4501 cgaagcggtc gcggttgtag aatttggggt tggcggggtt gtgattgagg aatttcgtcc
     4561 acaatgtttc cgccatttcc gccataccca taggcgcgcc ggggtggccg gaattggctt
     4621 tttgaacggc atcggccgag aggaagcgga ttgcgtttgc cagttgagac attttgtatt
     4681 ttccttgctg gtgtttcaga taagtggata atcggaaggc gttgattatc gcccgattcg
     4741 cttatgcttt caagaaaagg gcggacgcgg gggaaggcgg cggcagggga ccggtgggga
     4801 aggattttcg atttgcgggc gaagcctgcc attattcctt ttgaaataaa atgttataga
     4861 ttgtgtgccg gattgtcgat agcgtttgtt tatgagcttg cgccgtcggt tctgccgata
     4921 tgggggtgtc ggttttttta gtcttttttt taaccgtatt cggattttgt tcgggcggta
     4981 aggtaaaatc caggcgtttt gatgcggatg ggaaatgtat ccgccccata catccagacg
     5041 gcgcataaag ttgtacaata gcggaaatat attttgggaa aaacggattt tctcaaaact
     5101 tgaaacacaa cgccttaaaa aacaagaaaa atgacgtctg aaaagcaaac ggcggcggta
     5161 acgggcaaac catcaatcaa aaatttaagg aatgcagaat gaccacggaa aaccaagccg
     5221 gcagtccggc atccggaatc ggcacgtccg aacaaacaaa agccgcgccg aaagcaaaaa
     5281 aaacattcga tccgcgagcc agcgtgattc aaatccatcc cgaaggcgaa cgcatccatc
     5341 ccaaaaaggc ggaaggacgg ttcgccaaac tgcgtatcgc cgccgtattg gcgacgcagt
     5401 ttgtgtttta cgtcattccg tggttcaact ggagcggcag gcaggctgtc gttttcaata
     5461 tccccgaacg gcatttcttc attttcggat tgtcgttggg ggtgggcgat ttgatttacc
     5521 ttgccttgct gctgatgatt tgcgccttcg ggctgttttg gtggacgacg attgccgggc
     5581 gactgtggtg cggctattcc tgcccgcaaa cggtttacac cgaaattatg ctgtggatcg
     5641 acaacctggt cgaaggcgat agaaacaaac ggctgaagct ggaaaaatcg ccgtggaatt
     5701 tcactaaaat ccgcatcaaa gccaccaaat acctgttgat tttccttgtc tgcgcgtgga
     5761 cgggcatcac gtttgcaggc tggtttgtcc ctatccgcca gttcgttccc gatttattca
     5821 ctggagcagc aggcggcggc gcgatgtttg ccacagcgtt ttacggcttt atgaccttct
     5881 tcttcgccca cattatgcgc gaaaaagtat gcctgcatat gtgtccgtat gcacgtttcc
     5941 aaagcgcgat gttcgacaag gacacgctga ttgtttctta tgacgcggaa cgcggcgaac
     6001 cgcgcggcgc gcgcaagaaa acggtcaaca aggaagaggc gggtttgggc gactgcatca
     6061 actgtgcgat gtgcgtccaa gtctgccctg tcggcatcga catccgcaac ggtctgcagt
     6121 accaatgtat cggctgcgcc gcctgtatcg acgcgtgtga tgagattatg gacaaaatgg
     6181 gctatccgcg cggattaatc cgttatacga ccgaaagcgc gctggaacac gaatattctg
     6241 aaaaagacat taaaaaacgg ctgctcagac cgcgtgtggc aggttacggc gcggtgttgg
     6301 cgttagttat cattgccttc ctggccggtt tgtccacgcg caaaatggtc gaggtcgata
     6361 ttttgaaaga ccgtggcgta ctggtgcgcg aaaacgccaa aggctggctg gaaaacgcat
     6421 acagcctgcg tatcatcaac aaaagtgaaa aagaacagct cattaccgca agtgtcaaag
     6481 gctttgacga aatcgccctg accgggctgc ccgaaggcgg tatcaaggtt gccccgcgcg
     6541 aaacggtaac ccttcccgtc caagtgtcca ccattccgga atacgcggac aaaggcagcc
     6601 accctatcga atttaccttc caataccgcg aaagcggcgc gcccgacggc aagccggtcg
     6661 tcttggaaga agatgcaacc tttatcggag aataaccgtg tctcaaaaca ctccaatcaa
     6721 accttggtac aaacacgtct ggccgtggat cttgatggcg gggccgattt ttgtcgtcat
     6781 cgccagcgtc gctatgtttt ttgtcgcgca gcagcacgcg acagatttgg ttacggacga
     6841 ttattataaa gacggcaaac atatcgacat ccagcttcat cgggatgaag aagccatcag
     6901 acggcatatc ggggtgcagg ttctcatttc ccccgatatg aatgcggcaa aagtgtttgt
     6961 cggcggcgag tttgacggca aacagccttt gaacctgctg ctgatgcacc cgacccgcaa
     7021 ggcggacgat caaaccgtcg ccctcaagcc cgtcggcagc gcgcagaacg gcagggcgga
     7081 atatgaggcg gtgttcaaaa ccctttcgcc gaccaaccac tggt
//
LOCUS       DAWHRA010000106         7115 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWHRA010000106 DAWHRA010000000
VERSION     DAWHRA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.106"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1908)
                     /locus_tag="WIB30_001623"
     CDS             complement(<1..1908)
                     /locus_tag="WIB30_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ3797662.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTDWANTVERLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDGGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGK"
     gene            complement(1996..2868)
                     /locus_tag="WIB30_001624"
     CDS             complement(1996..2868)
                     /locus_tag="WIB30_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ3797663.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPADSETL"
     gene            3138..5528
                     /gene="gyrB"
                     /locus_tag="WIB30_001625"
     CDS             3138..5528
                     /gene="gyrB"
                     /locus_tag="WIB30_001625"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ3797664.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIETNEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            5625..7010
                     /locus_tag="WIB30_001626"
     CDS             5625..7010
                     /locus_tag="WIB30_001626"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ3797665.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNSLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPSGLNVRRRVPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
BASE COUNT         1605 a         1986 c         2036 g         1488 t
ORIGIN      
        1 tttgcctgtg gcggcgacat cgtcgctttc ttcaaaggcg gcgtatgcgt ccgcgctcaa
       61 tacttccgcc cacgcgtagc tgtaatagcc tgcggaatag ccgcctgcga agatgtggcc
      121 gaagctcaag gcgaagcggt tgtattcggg cggctggatg acggcgactt ttttgcgcac
      181 gctgtctaaa acctgttgcc agtttttcag acggccttcg tcgtcttcgc tgtaaatcat
      241 catatcaaag agggcgaact ccatttgccg gacgaggaac atgccgcgtt ggaagttttt
      301 ggcggcgagc attttgtcga agagttcttt cggcagggga acgccggttt cttcgtgggc
      361 ggacatttgt gccaagacat tgtattccca aacgaaattt tccataaact ggctgggcag
      421 ttcgaccgcg tcccattcta cgccgttgat gccggatacg cccagttcgt ccacttgggt
      481 aagcaggtgg tgcagcccgt gtccggtttc gtggaagagg atgaggattt cgtcgtggct
      541 caggcgggct tccctgccgc cgacgggtgg ggcgaagttg cagacgaggt aggcggtggg
      601 cagttgcagc gtgccgtctg aaaaacggcg gcggcctttg tagtcgttca tccacgcgcc
      661 gccgcgtttg ccttcgcgtg cgtacaaatc catataaacg ccgcctatgg tttcgccgtt
      721 ttgttgcaat tcaaaatagc gcacgtcttt gtgccagacg gggacggttt tttcggtaaa
      781 tccgatgccg tagagttttt tgatttgggc gaacagtccg tttaatactt tgccgacggg
      841 gaagtatttt ttgacttcgg tttcgctgaa cgcgtatttg gcttcgcgca gtttttcgct
      901 ggcgtagccc aagtcccacg gttgcaaatc ggcgaggttc aggctttcgc gggcgaaggc
      961 tttgacttcg gcgaggtctt tttcggcgta gggtttggcg cggcgggcaa ggtcgtgcag
     1021 gaagtttaaa acttgttcgg gggtgtccgc cattttggtt gccagcgaca attcggcgta
     1081 gtttttgaag ccgagcagtt tggcggtttg cagggcgttt gcgagcgtgc ggtcgatgtt
     1141 ggcggtgttg tcgaatttgc cgccgtctga cagttcgctg gcgcgggtaa cgtaggcgcg
     1201 gtagatttgt tcgcgcagtt cgcggttgtc ggcgtattgg atgacggcga ggtagtgcgg
     1261 aatctgcaaa ccgattttgt agcctgtttt gccttcgctt tgcgcggcag cggcaaacat
     1321 ggcgagcgcg tcttcgggaa tgccggcaag cggtgcggca tcgtcaaagt aaatgccgaa
     1381 cgcgtcggtc gcgtctagga cgttttggga gaatttggcg gaaagttgcg cgccttcggt
     1441 ttgcagtttt gccagttctg cctgctgttc gggcggcagt tccgcgccgc tgaggacgaa
     1501 atcgcgcaga tcgtggttga gtttggtttt ttgtgcgggg gagagggtgt cgaattcggg
     1561 ggaatttttg atggttttga agcggttgta cagctcgatg tcttgtccga tttcggtgaa
     1621 gaagacggtg atttcgggca tcagttcgtt atagacggcg cgcagttcgg gcgtgtcggc
     1681 gacggagttg aggtgcgaca ccacgcccca aatcctgccg acgcgctcgg tgatgccggt
     1741 cagacgctcg acggtgttcg cccagtcggt gtgtgtttgt gctttgatgg cggcgatttg
     1801 ttcgcgcgct tcggcgatgg cggtttgcag ggcgggtttg atgtcttcgg ttttgatttg
     1861 atcaaaacgg ggttcttcgc ccaaatggag cagtgcgttg tcagtcataa gatgggtttc
     1921 ctttgcgtgt tgtgcttcag acagcatatc ggcaatatgc cgtctgaagc cggaaatggg
     1981 gtcggtcggg gaaagttaaa gtgtttcgga gtcggcggga tgatttaaca ggcggtattg
     2041 tacgcgcaaa aattcgtcca taaccgcctg ttggatttcc agccgctttg aaacggggga
     2101 ggcgaagcgg acgatgatgc ggtatgcctt gtcgtcatac ggtacgcggg taacgcgcgg
     2161 tctggcggcg ggcgtgataa acagtttttc cgcctgcacg ttttccaaat gccgctggat
     2221 ggcggggatg tagggcgcgc acaagggctc gagtacggct ttcagacggc atacggcctc
     2281 atccgaatcc aaatggatgg ggacggggat ttcgaccgta tggatgacgt agtcgcccaa
     2341 aatattgtcg cggcgcacgg ggtggcttaa caacaggctg ttggggaaag aaacggtggt
     2401 tcccgcaagc tgtccgacca aggggttcgg gccgacctgc atcatcagcg tgttcaacag
     2461 gttgatgtcg accacgcgcc cgcgcaggcc gttgatttcg atatagtcgc cgaccgagta
     2521 ttgctgggtg gcagacctta aaatactgcc cgacagacac ataatcagtt ccttcgtcgc
     2581 cacgacgacc gccgccgcca ccgcaaacat cgacaaagcc agcgtttgaa tttgcgccga
     2641 ccagataaat gccagcgaaa acagcaccaa aagcagcgtt atattgcggc tggcaaccaa
     2701 aaaccgccgc ttgctttcga tgccgaaatc cggatgccgt ttgaagtgga tattcaacag
     2761 aagggcgcgc gccagcagca aagccgcaac cgccgccacg gattcgaccg cctccgcacg
     2821 tatcgggacg gcaccgagcc aagtgtccaa catattccat atttccattt ccgcgcccct
     2881 gtccgaaaag tatagggtgt agattttagt ggcaaaaaaa cgcttttgcc actttacggg
     2941 acaatccccg acctgatgcc ggaggcaaac gtaccgtttc atcttttccc ggcggcacac
     3001 tgatgcggat aacgctgaat taggcggcaa aatcggctat aatacacaat tatatatgtc
     3061 gtcccgcccg ctttttctac aaggtgcgga cttgttttgg cggcatcgca aaatcttttc
     3121 aaaatccggc aaaaaatatg actgaacaaa aacacgaaga atacggcgcc gacagcattc
     3181 aggtgctcga aggcttggaa gcggtacgca aacgccccgg catgtacatc ggcgacacgc
     3241 aggacggcag cggtctgcac cacatggtgt tcgaagtatt ggacaacgcc attgacgaag
     3301 cactcgccgg acattgcgac aaaatcacgg taacgataca cgccgaccat tccgtcagcg
     3361 tcgccgacaa cgggcgcggt atgcccaccg gcatccaccc gaaagaagga cgctccgccg
     3421 ccgaagtcat catgaccgta ttgcacgcgg gcggtaaatt cgacaacaac agctacaaaa
     3481 tctccggcgg cctgcacggc gtgggcgtgt ccgtcgtcaa cgcgctgtcc gactgggtaa
     3541 cgctgaccat ctaccgcgac ggcaaagaac acttcgtccg cttcgtgcgc ggcgaaaccg
     3601 aagagccgct gaacgttgtc ggcgattccg ataaaaaagg cacgaccgtg cgcttcctcg
     3661 ccagtacgga aaccttcggc aacgtcgaat acagcttcga catccttgcc aaacgcatcc
     3721 gcgaactttc gttcctgaac aacggcgtgg acatcgaatt gaccgacgag cgcgacggca
     3781 aacacgaaag cttcgccctt tccggcggcg tggctggatt cgtgcaatac atgaaccgca
     3841 aaaaaacgcc cttgcacgaa aaaatcttct acgcgttcgg cgagaaagac ggcatgagcg
     3901 tcgaatgcgc gatgcaatgg aacgacagct atcaggaaag cgtgcagtgc ttcaccaaca
     3961 acatccctca gcgcgacggc ggtacgcacc tgaccgcgtt gcgccaagtg atgacgcgca
     4021 ccatcaacag ctacatcgaa accaacgaag tcgccaaaaa agccaaagtg gaaaccgccg
     4081 gcgacgatat gcgcgaaggt ttgacctgcg tgttgtccgt caaactgccc gaccccaaat
     4141 tttcgtccca aaccaaagac aaactggttt ccggcgaaat cggtcccgtc gtcaacgaag
     4201 tcatcagcca agccctgacc gacttcctcg aagaaaaccc tgccgaagcc aaaatcatca
     4261 ccggcaaaat cgtcgatgcc gcccgcgcgc gcgaagccgc ccgcaaagcc cgcgaaatca
     4321 cccgccgcaa aggcgtgatg gacggtttgg gcctgccggg caaactcgcc gactgccaag
     4381 aaaaagaccc cgcattgtca gaactttatc tggtcgaggg cgattctgca ggcggttccg
     4441 ccatgcaggg gcgcgaccgc aaattccaag cgattttgcc gcttaagggc aagattttga
     4501 acgtcgaaaa agcgcgcttt gaaaaaatgc tcgccagtca ggaagtcgcc gctttgatta
     4561 ccgcgttggg cgcgggcatc ggcaaagaag aattcaatgc cgagaagctg cgttaccacc
     4621 gcatcattat catgaccgat gccgatgtgg acggtgcgca tatccgcacc ttgcttttga
     4681 ccttcttcta ccgccaaatg cccgaactgg tcgagcgcgg ctacatctat atcgcccagc
     4741 cgcctttgta taaagcgaaa tacggcaaac aggaacgtta cctcaaggac gagttggaaa
     4801 aagaccaatg gctgctcggt cttgccttgg aaaaagccaa aatcatttca gacggccgca
     4861 ccatcgaagg cgcagaactt gccgacaccg ccaaacaatt cctgttggca aaaaccgtca
     4921 tcgaacagga aagccgcttc gtagacgaac tcgtcctgcg cgccatgcta cacgcgtcgc
     4981 ccattgattt gacgtcgtct gaaaacgccg ataaagccgt tgccgaactt tccggtctgc
     5041 ttgacgaaaa agaagtcgcc ctcgaacgca tcgaaggtca tgaaggacac cggttcatca
     5101 aaatcacgcg caagctgcac ggcaacgtca tggtcagcta catcgaaccc aagttcctca
     5161 acagcaaagc ctaccaaacc ctcacccaaa ccgccgccgc gctcaaaggc atggtcggcg
     5221 agggtgccaa gctttacaaa ggcgaaaacg ggtacgacgc ggacagcttt gaaaccgctt
     5281 tggacatctt gatgagcgtt gcccaaaaag gtatgtccat ccaacgatac aaaggcttgg
     5341 gcgagatgaa ccccgagcag ctgtgggaaa ccacgatgga tcccgccgtg cgccgcctgt
     5401 tgaaagtgcg catcgaagat gccattgccg ccgacgaagt gttcgttacg ctgatgggcg
     5461 acgaggtcga gccgcgccgt gcctttatcg aaaacaacgc gctgattgcc caaaatatcg
     5521 acgcataagt gccgttttaa aaaaggagac gggcatcgtg ccgcgtctcc tttttggttg
     5581 gtcaaacgga acctgtgccg tctgaaaaac cgtcggagca aaatatgatc agcattttcg
     5641 atattttcaa aatcggtatc gggccttcca gttcgcatac ggtcggcccg atgaaggcag
     5701 ccgccgcctt tgcggcaggt ttggatgcac aggctgttcg catcgtcatc gacatttacg
     5761 gctcgctcgc actgaccgga tacggacacg gtacatttga cgcgctgatg ctcggtttgg
     5821 aaggcagcct gccgcacgac atcccgcttg ccggcattcc cgaacgcctc gaacgcatcc
     5881 gcacgcagca catcctccgg ctcaacgggc aagaaatccg cttcatcccc gaccgcgacc
     5941 tgaacatact cggcaatcaa gtgctgccca aacaccccaa cagcctgcgt tttaccgcct
     6001 atgcttcaga cggcacggta ttgaatgaac aggtttatta ttcggtcggc ggcggctttg
     6061 tcgttaccga agaagatttt gaccggcagg cggaaacgga aaaagccgtt ccctatccct
     6121 ataccagttg cgccgaactg cttgcccgat gccgtctgaa ccggctcgac atctccgaag
     6181 tcgtgttggc aaacgaagcc gcgcttgccg gatgcggcga agccgaaatc cgccgccgcg
     6241 ccgctgccgt tgccgaggtt atggaaggct gcatcaaacg cggcttgggt gcggacggcg
     6301 aactgcccag cggattgaac gtccgccgcc gcgtcccgca gcttgccgcc aagctcaaag
     6361 tcctgcgcga aaccgaaatc gtcaacaccc agctctggcc gatggtgtac gccatggcgg
     6421 tcaacgaaga aaacgccgcc ggcggacgcg tcgttaccgc accgaccaac ggcgcggcag
     6481 gcatcattcc cgccgtattg cactatttcc gcaagttcaa tccgcacgcc acacaggaac
     6541 gcgtcgaaaa cttcctgctc accgcaggcg caatcggcat cctctacaag accaacgcct
     6601 ccatttccgg tgcggatgtc ggctgtcagg gcgaagtcgg cgtagcgtgt tcgatggcgg
     6661 cgggcgcata cgccgaagtc atcggcggca cgcccaaaca agtggaaaac gccgccgaaa
     6721 tggcgatgga acaccatttg gggctgactt gcgaccccgt cggcggactg gtgcaaatcc
     6781 cctgcatcga gcgcaacggc atcgccgccg aaaaagccct caaactcggc acgctcgcgc
     6841 ttttggaaga cggcacggac aaaaaagtct cgctcgacga agtcatccga accatgctgc
     6901 aaaccggccg ggatatgaag gcgacctaca aagaaacctc gcttgccgga ctcgccgcca
     6961 cgctccggaa aaaagccgtc cccgtatccg tgcgcgtggt cgagtgctga ggatggacag
     7021 aaacgaaaat gccgtctgaa aacagttttt tagacggcat ttcatttatt tcttgcggct
     7081 ttaccgtttc agtgccagcg tcaacacgcc cgccg
//
LOCUS       DAWHRA010000107         7071 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWHRA010000107 DAWHRA010000000
VERSION     DAWHRA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.107"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            83..1048
                     /locus_tag="WIB30_001627"
     CDS             83..1048
                     /locus_tag="WIB30_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3797666.1"
                     /translation="MLRLTALTLCTVLALGACSPQNSDSAPQAKEQAVSAAQTESASV
                     TVKTARGDAKVPQNPERIAVYDLGMLDTLNKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            complement(1134..1241)
                     /locus_tag="WIB30_001628"
                     /pseudo
     CDS             complement(1134..1241)
                     /locus_tag="WIB30_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1382..2350
                     /locus_tag="WIB30_001629"
     CDS             1382..2350
                     /locus_tag="WIB30_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ3797667.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFVGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDILGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2340..3314
                     /locus_tag="WIB30_001630"
     CDS             2340..3314
                     /locus_tag="WIB30_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ3797668.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTLGGV
                     GYASLPLTGKFGFELIVMMGGSLLLFYTLIKQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHNELLGIGALILLISAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3359..3985
                     /locus_tag="WIB30_001631"
     CDS             3359..3985
                     /locus_tag="WIB30_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797669.1"
                     /translation="MTQERLPDFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAFMQGERDEGTTGVTASVVQLLTS
                     AAPETGFGGVGPAGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVSGSASAEELEHFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            4068..4826
                     /locus_tag="WIB30_001632"
     CDS             4068..4826
                     /locus_tag="WIB30_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3797670.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLADTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKIALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(4885..5223)
                     /locus_tag="WIB30_001633"
     CDS             complement(4885..5223)
                     /locus_tag="WIB30_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ3797671.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            5318..5530
                     /locus_tag="WIB30_001634"
     CDS             5318..5530
                     /locus_tag="WIB30_001634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797672.1"
                     /translation="MPETVIPAQVGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            5687..>7071
                     /locus_tag="WIB30_001635"
     CDS             5687..>7071
                     /locus_tag="WIB30_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ3797673.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKRQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTN"
BASE COUNT         1552 a         2083 c         1858 g         1578 t
ORIGIN      
        1 attatcattt atcctttcta aagccgttcc ggtttgtccg accgacggct ttgccccaat
       61 atccccattt tggagacacc ctatgttgcg tttgaccgca ttgaccctct gcaccgtcct
      121 cgccttgggc gcgtgttcgc cgcaaaattc cgactctgcc ccacaagcca aagaacaggc
      181 ggtttccgcc gcacaaaccg aaagcgcgtc cgttaccgtc aaaaccgcgc gcggcgatgc
      241 aaaagtgccg caaaaccccg aacgcatcgc cgtttacgat ttgggtatgc tcgacacctt
      301 gaacaaactg ggcgtgaaaa ccggtttgtc cgtcgataaa aaccgcctgc cgtatttaga
      361 ggaatatttc aaaacgacaa aacctgccgg aactttgttc gagccggatt acgaaacgct
      421 caatgcctac aagccgcagc tcatcatcat cggcagccgc gccgccaaag catttgacaa
      481 attgaacgaa atcgcgccga ccatcgaaat gaccgccgat accgccaacc tcaaagaaag
      541 tgccaaagag cgcatcgacg cgctggcgca aatcttcggc aaacaggcgg aagccgacaa
      601 gctgaaggcg gaaatcgatg cttcgtttga agcggcgaaa actgccgcac aaggcaaagg
      661 caaaggtttg gtgattttgg tcaacggcgg caagatgtcc gccttcggcc cgtcttcacg
      721 actgggcggc tggctgcaca aagacatcgg tttgcccgct gttgacgaag ccatcaaaga
      781 aggcagccac ggtcagccta tcagctttga atacctgaaa gagaaaaatc ccgactggct
      841 gtttgtcctc gaccgaagcg cggccatcgg cgaagagggt caggcggcga aagacgtgtt
      901 ggataatccg ctggttgccg aaacaaccgc ttggaaaaaa ggacaggtgg tttaccttgt
      961 tcctgaaact tatctggcgg ccggcggcgc gcaagagctg ctgaatgcaa gcaaacaggt
     1021 tgcggatgcg ttcaacgccg cgaaataatg aaacggcggc attcgatgcc gtctgaaaca
     1081 cggatgcaaa ccgcttcccg tgtttcagac ggcattgccc gatacgggcg ttatagtgga
     1141 ttaaatttaa accagtacgg cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc
     1201 ttcgtcgcct tgtcctgatt taaatttaat ccactatatc gcgggaatgg cggcaagcgt
     1261 ttttattgga acttgcccta tcctctgccc gatacggagg cttcaaacaa ggctttccgc
     1321 tccgacggtt cggactgcct tgtttgaatc ttctacgcct taacgctttt cccttctgtt
     1381 tatgactgcc aaaccttttt ccctcaacct gaccaacctc ctgctgctgg cggtgttgtt
     1441 tgccgtcagc ctgtcggtcg gcgttgccga tttccgctgg tcggatgtgt tttcgctgtc
     1501 ggacagccag caggttatgt tcatcagccg cctgccgcgc acgtttgcga ttgtgctgac
     1561 gggcgcgtcg atggcggtgg cggggatgat tatgcagatt ctgatgcgta accgttttgt
     1621 cgaaccttct atggcgggcg cgggtcagag tgcggctttg ggtttgcttc tgatgtccct
     1681 gctgctgcct gccgcgccgc tgccggtcaa aatgtcggtt gccgccgttg ccgcgttaat
     1741 cgggatgctg gtgtttatga tgcttatccg ccgtctgccg ccgaccgcgc aactgatggt
     1801 gccgctggtc gggattattt tcggcggcgt ggttgaggcg gtggcgacct ttatcgcgta
     1861 tgaaaacgaa atgctgcaaa tgctcggcgt gtggcagcag ggcgattttt cgagcgtgct
     1921 gctggggcgg tacgagctgc tttggattac gggcggtttg gcggtgtttg cctatctgat
     1981 tgccgaccgg ctgacgattt tggggctggg cgagacggtc agcgtgaatt tgggtttgaa
     2041 ccggacggcg gtgttgtggt cggggctgat tattgtggct ttgattacgt cgctggttat
     2101 cgttacggtc ggcaatattc cgtttgtcgg gctggtcgtg ccgaacatca tcagccgcct
     2161 gataggcgac aggctgcgcc aaagcctgcc tgcggtggcc ttgttgggcg cgtctttggt
     2221 gttgctgtgc gatattttgg gacgcgtgat tgtgtttccg tttgaaattc cggtctctac
     2281 ggtttttggt gtgttgggta cggctttgtt tttgtggctt ttgttaagga aacctgctca
     2341 tgccgtctga aaaaaatatc ggttttatgg caggaagcag ccgtccgttg tgggttgcct
     2401 ttgcgctgtt gctggtttcc tgcatcctgt ttatgacgct caacgtcaaa ggcgattggg
     2461 actttgtact gcacctgcgt ctgaccaaac ttgccgcgct gctgatggtc gcctatgcgg
     2521 tcggcgtgtc cacgcaactc ttccaaacgc tgaccaacaa tccgattctg accccttcaa
     2581 ttttgggttt cgattcgctg tatgtgtttt tgcagacctt gctggtgttt acgctcggcg
     2641 gcgtgggcta tgcttccctg ccgttgacgg gcaaattcgg ctttgaactg atcgtcatga
     2701 tgggcggctc gctgctattg ttctacacgc tcatcaaaca gggcggacgc gatttgccgc
     2761 gtatgatttt aatcggcgtg attttcggga ttttgttccg cagcctgtca tccctgcttt
     2821 cgcgcatgat agaccccgaa gaatttaccg cggcgcaggc gaatatgttt gccggattca
     2881 ataccgtcca caacgaactg ctggggatag gcgcgctgat tctgctcatc agcgcggcgg
     2941 cggtttggcg cgaacgctat cgcttggacg tacacctttt ggggcgcgac caagccgtca
     3001 atttgggcat cagctacacg cgcaacacct tatggatact gctttggatt gccgcattgg
     3061 tggcgacggc gaccgccgtg gtcggcccgg taagcttttt cgggcttctc gccgcctcgc
     3121 ttgccaacca cttttccccg tccgtgcgcc attccgtccg cctgccgatg acggtttgtg
     3181 tcggcggtat cctcctggtc ggcggacaaa ccgtattcga acacttcttg ggcatgaagg
     3241 cggtattaag cgtggtggtc gaatttgcgg gcggactcgt tttcctctat ctcgttttaa
     3301 aacacaaaaa atgacggatg ccgtctgaac ggcagcccgc cccgaaagga caaaccatat
     3361 gacgcaagaa cgtttacccg attttttcga ccgcgccccg acgctgaccg tacaagaccc
     3421 gcttgccgca ttcctcggcg cggccgaaaa cggcatcctc acttaccgct acgccgatgc
     3481 cgtgcgcctg tgcggacatt cctgcccgac cgtcgcgggc gcgtacctga tggttatcaa
     3541 aggtctgaaa gccctttacg gcgaagagct gcccgaacgc ggcggcatcg aagcctttat
     3601 gcagggtgag cgagacgaag gcacgaccgg cgtaaccgcg tccgtcgtcc aactcctcac
     3661 cagcgcagcc cccgaaaccg gctttggcgg cgtaggcccg gccggacgct tcgcccgccg
     3721 caacctctta tcctttggtg caggcgaaat caacggcaca ctgaccctgc gccgcaaaga
     3781 caacggcaaa accgtcgccg tcagcctcaa cgccgccctg caacccttcg caccgcaaat
     3841 gcgcgacatc atgcccaaag ccgtcagcgg cagcgcaagc gcagaagaac tcgaacactt
     3901 cggacaactc tggcaggcac gcgttaaagc atttttgacc gaatcggcgg acgacccgca
     3961 gttcgtcatc gtccgcgaag tgtgagcgtt cagacggcat tccgaatttc aaatgccgtc
     4021 tgaaccccgc caaacaacaa caaacctacg cccgacaagc atccgccatg attaccatcc
     4081 gcaacgtcag ctaccgcatc ggcacacgcc ccatcctcga caacgtcagc ctcgacatcc
     4141 ccgaaggcgg cattaccgcc ctcgtcggcc ccaacggcgc gggcaaatcc accctgtttt
     4201 cctttatggc gcggctgcgc ccgcttgaaa gcggcagcat cgcctaccgc ggcaaaaacc
     4261 ttgccgacac ccccaccgcc gaactcgccc gcacgctctc catcctcacc caagaaaaca
     4321 gcatcatgag ccgcattacc gtgcgcgacc tgctgatgtt cggccgttac ccctaccatc
     4381 aaggcagacc gactgccgaa tgccgccgta tcgttaacgg tgcaatcgaa gaattccacc
     4441 tgcaagacct ctccgaccgc tatctgaccg agctctccgg cggacaacgc caacgcgcca
     4501 tgattgcgat ggtgttctgc caaagcaccg actacgtcct tttggacgaa ccgctgaaca
     4561 acctcgatat gtatcacgcc cgctcgctca tgcagattct gcaaaagctc acgcgcgaac
     4621 acaaacgcac caccgtcgcc gtattgcacg acatcaacca ggccgccgcc tacgccgacc
     4681 acgtcgtcgc catgaaaaac ggcaaaatcg ccctgaccgg cacgcccgaa gaagtgttca
     4741 ccgtacacaa catcaaagaa ctgttcgaca tggacgtgga cgtactcgac tacgaaggca
     4801 aaaaactcat cgtccaccat atctgaagct caaaaagaaa atgccgtctg aaaacctttc
     4861 agacggcatt tccattccaa acgctcaaac cgccgcgtcc gcacgttcgc ccgtgcggat
     4921 acggattgcc tcctcgaccg gcagcacaaa aatcttgccg tcgccgattt ttcccgaacg
     4981 cgccacctcg acaatcacgt caatcgcgcg ttccacagca tcatccgcca acaccagctc
     5041 gattttgacc ttgggcagga aatcgacggc gtattccgcg ccgcgataga tttccgtatg
     5101 ccccttctgt ctgccgaacc ctttgacctc gctgacggtc atgcccgtaa tgccgatttc
     5161 cgtcaacgcc tcgcgcacgt cgtcgagttt gaacggtttg acaatcgcct cgattttttt
     5221 catcacattc tccatgaatt aacgtaaaca aacgacaaat atagcagaaa ccgccggcaa
     5281 acgataaacg gtactgttcg cccggcgatg cggcgatttg ccggaaaccg tcattcccgc
     5341 gcaggtggga atcccgatct ctgcatttca gcaatattta aaggtttctg taaatccaaa
     5401 tttccatatt cctaaccgtg cggaaacgac ggcatttgat ctaaaaacat acggaagcag
     5461 ttgcccgccc ctctacttcc tgcaattatc cgcatacccg gctttgggta aaaatcagaa
     5521 aaacagataa aacccttatt ccttaatatc ttcagacggc acctgtattt tccccaactg
     5581 tatgccgtct gaagattgtc aacattttaa gccaaaatac ccaagcaata cagccccgtt
     5641 gcgcgtataa tgacagattt tccaaccgca tttgagagcc gaatccatgt ctgtcgtttt
     5701 gcccttgcgc ggcgttaccg ccctttccga tttccgtgtc gaaaaactct tgcaaaaagc
     5761 cgccgcactc ggactgcccg aagtcaaatt aagcagcgaa ttttggtatt tcgccggcag
     5821 cgagaaagca cttgatgccg cgactgtcga aaaactgcaa gccttgttgg cggcgcaaag
     5881 cgttgaacaa acgccaaaag cgcgcgaggg cttgcatttg tttttggtaa cgccccgttt
     5941 gggtacgatt tcgccgtggg cttccaaggc gaccaatatc gcggaaaact gcggtttggc
     6001 aggcatcgag cgcatcgagc gcggcatggc ggtgtggctg gaaggtgcgc ttaccgatga
     6061 acagaaacgg caatgggcgg cgttgctgca cgaccgcatg accgaatcgg tgctgcccga
     6121 ttttcagacg gcatccaaat tattccacca tctcgaatcc gaaacctttt ccaccgttga
     6181 tgttttaggc ggcggtaaag aggctttggt caaagccaat accgaaatgg gtttggcact
     6241 ctccgccgac gaaatcgatt atctggttga aaactatcag gctttgcagc gcaatccgtc
     6301 cgatgtggaa ctgatgatgt tcgcgcaggc aaacagcgaa cactgccgcc acaaaatctt
     6361 caacgccgat ttcatcctca acggcgaaaa acaaccgaaa tccctgttcg gcatgatacg
     6421 cgacacgcac aacgcgcatc ccgaaggtac ggtcgtcgcc tataaagaca attcgtccgt
     6481 gatcgaaggc gcgaaaatcg agcgtttcta tccgaatgcg gcggaaaacc aaggctaccg
     6541 tttccacgag gaagacacgc atatcatcat gaaagtggaa acgcacaacc acccgaccgc
     6601 catcgcgccg tttgcaggcg cggcaacggg agcgggcggc gaaatccgcg acgaaggcgc
     6661 aacgggcaaa ggttcgcgtc cgaaagcagg cttgaccggc tttaccgtgt ccaacctcaa
     6721 catccccggc ctcaaacagc cgtgggaaca agattacggc aagccggaac acatttcctc
     6781 gccgctggac atcatgatcg aaggtccgat cggcggcgcg gcgttcaaca acgaattcgg
     6841 ccgccccaac ctcttgggct acttccgcac ttttgaagag aagtttgacg gtcaggttcg
     6901 cggctaccac aaaccgatta tgattgccgg cggcttgggc agcattcagg cgcagcagac
     6961 gcataaagac gaaatccccg aaggcgcgtt gctgatccaa ctgggcggcc cgggcatgct
     7021 tatcggcttg ggcggcggcg cggcttcttc gatggatacc ggcacgaacg a
//
LOCUS       DAWHRA010000108         6953 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWHRA010000108 DAWHRA010000000
VERSION     DAWHRA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.108"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            304..1737
                     /locus_tag="WIB30_001636"
     CDS             304..1737
                     /locus_tag="WIB30_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ3797674.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFLIKENKEALARLMTMEQGKGLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEVEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1847..2635
                     /locus_tag="WIB30_001637"
     CDS             1847..2635
                     /locus_tag="WIB30_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ3797675.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            3003..4559
                     /locus_tag="WIB30_001638"
     CDS             3003..4559
                     /locus_tag="WIB30_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ3797676.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4576..5322
                     /gene="surE"
                     /locus_tag="WIB30_001639"
     CDS             4576..5322
                     /gene="surE"
                     /locus_tag="WIB30_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ3797677.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
     gene            5578..6825
                     /locus_tag="WIB30_001640"
     CDS             5578..6825
                     /locus_tag="WIB30_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ3797678.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKAAAVK
                     SRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT         1605 a         1820 c         1950 g         1578 t
ORIGIN      
        1 gttcggattt ttgccatact ctaccacacg ttgcaactgc aatctttgct ccttatatat
       61 tgcaccccat caaagggctg cattactttt ctttctaaag attactatcg tataaaaaat
      121 atttttctta aacgacaggg attgcgcccg cgccatattc agacaccgcc cggatgttgc
      181 gctgcccgat cggatgcttc agacggcatc ggaagggttt gcagttttgg aatatgaaga
      241 tgataatgtc cgcgagattg acggcatttg aaagcggtta ttccgcatac cggaggatac
      301 gaaatgaacg aatattccca attaatcaag catcccgata tttcccttcc cccggtttca
      361 gacggcatca aagtcgataa tccggcaacg ggcgagactt tggcgtttgt ccgcaagacg
      421 gattcggaca agctgaaaaa cctgattcaa aaggcggcgg cagcgcaaaa attatgggcg
      481 gcaaaaaccg cgttggaacg cgccgatgtg ttgtggcgtt ggtatttttt gataaaggaa
      541 aacaaagaag cactggcgcg tctcatgacg atggagcagg gcaaaggcct gaccgaggcg
      601 cgcggcgaaa tcgattatgc ggcttcgttt gtgcgctggt ttgccgaaga ggcgcggcgg
      661 attgacggcg atgtgctgac gagtgtgaaa gcgtcgcaaa aactggtcgt gttgaaacag
      721 cctgtcggtg ttaccgccgc gattacgccg tggaactttc cgtccgcgat gattgcgcgc
      781 aaggctgcgc cagctttggc agtgggttgc gcgatgatag tcaaacccgc gtcgctcacg
      841 cctttgagcg cgtatgcgtt ggccgtgttg gcttacgaag cgggcgtgcc gcaggatttg
      901 ctgcctgttg tcagcggcag tgcttcggaa atcggccgtg aatttgccac aaatccgatt
      961 atccgcaaaa tcagtttcac cggctctacc gaagtcggcg cgaaaatttt tgccgacagc
     1021 gcggcagaca ttaaaaaact cagcttggaa ctgggcggca acgcgccgtt tatcgtgttt
     1081 gacgatgccg atttggacaa agccgtcgaa ggtgcgctcg ccagcaagtt ccgcaacagc
     1141 ggtcagacct gcgtctgcac caaccgcgtt tacgcccaat cgggcattta cgacgcattt
     1201 tgccgcaaat tgagtgaaaa agcagccgcg ctcaaattgg gcaacggctt ggaggatggc
     1261 gtcaaccaag ggccgctgat tgaggaaaaa gcggtagaaa aagtcgagca gcacatcgcc
     1321 gacgcgctct caaaaggcgc ggtctgtctg accggcggca aacgcagcgc attgggcgga
     1381 acgttttttg agccgaccgt cttaagcggc gtaacggcgc aaatggcggt ggctcgcgaa
     1441 gaaaccttcg ggcctttgtg tccggtattc cgttttgaaa ccgaagtcga ggtcatcgag
     1501 gctgcgaaca atacggaata cggtttggcg gcctaccttt tcaccgccga caccgcccgc
     1561 caatggcgcg tcggcgaagc cttggaatac ggtatggtcg gcatcaatac gggcttaatc
     1621 agcaatgaag cggcaccgtt cggcggcgtg aaacgcagcg gtttgggacg tgaaggcagc
     1681 aaatacggtg cggacgaata tctggaattg aaatatctgt gtatggatgt cgggtgacgg
     1741 atgccgtctg aacggcaggg tttcagacgg tgccgcattt taagcagtct ctatctgttg
     1801 tacaatgcgc cctgttttta cggttatttt tgatgtgaat taagatatga tggaaaacgg
     1861 aaaaacagtc ccgagatggc gttttgcctt gaaaagtgcg ggctggcacc tcttaatcag
     1921 cctgtcggtt gcagggctgg cggcattgct ggtttttaag gtttggtatc cttatcctta
     1981 tgccgagctg acgggagggc tgtcgcttta tcagctggtg gtggctgtcg atattgtatg
     2041 tggtccgctg ctgactttaa ttttggcaag cccgaagaaa aagacaaagg cacgcatggt
     2101 cgatttttcc atggtcggca tcatccagct ggcggctttg gtgtacggtc tgcacagcgt
     2161 ttctttggcg cgtcccgtgg tggaggcgtt tgaaaaagac aggattaatg ttgtgaccgc
     2221 cgccgaagtg gcggttgaag acctgcacaa agcgccggaa gggctgcaaa gcctgtcgtg
     2281 gttcggcatc cgccgcattg cattgaaaga acctgaggat gcggatgaga agaacaagac
     2341 gctggatttg tccctgaaag gtatcgagcc gagtatgcgt cccgaccggt ggctgccgta
     2401 ttccgacaag gaagcagaag aaatccgcaa acatctgaaa ccgctgaaag tcttggcgga
     2461 tgcgagaaaa acgacggttg cggacattct gaaacaggca ggtctcgccg aaggggagga
     2521 gctgtattac ctgccgttta ccagcagcag gcagaaagag tggatagtca ttaccgataa
     2581 agagggcaac accaaaggct acgcgccgat agacggcttc atcatcaccc cttaagcgtt
     2641 gggactccgt ccgcactcga acatccgttc ttcgcggcgg tagaatcaga ctgtatttga
     2701 gaggggcgca acccctcaat cagggcaaca cataggggaa ggctttatgt gccgtccgtg
     2761 tgttgaaaca tttcaaaata agctcatgcc gtccgaacat cctttcagac ggtatggcat
     2821 tttgccattg ccccgataag ctgttaaact atttaaatta tttcgaccga aggtacacgc
     2881 acatgaaaga ccccgagcag agcagcaagc ccgcccgccg ttttttgtgc gtaccgtctg
     2941 ttcaggcagc ttgagcgctt caggctgcct tttcgcgttt gaacttgagg aatacgaaaa
     3001 tcatggattt cagttggttg gcagaaccgc atacctggat aggttttgcc acgcttttgg
     3061 tgttggaagt cgtattgggg atagacaacc ttgtctttgt ggcgattttg gcaaacaagg
     3121 tccagcccgc acggcgcgac cgcgcacgga ttatcgggct ggggctggca gtcgtcatcc
     3181 gcatcattat gcttgctttt atggcgcaca tcatcacgct gaccgagccg ctgttccaaa
     3241 tcggcggcct cgccgtttcc ggcaaggaca tgattatgct cgcgggcggt attttcctgc
     3301 tttacaaagc caccaccgaa ctgcatgaac gcctcgaagg gcacaaccgt tttaccgttg
     3361 ccgacaacca aaaaaaacac gcgccgtttt ggggcgtggt cgcgcaaatc ctgatactgg
     3421 atgccgtgtt ttccatcgat tcggtcatta ctgcggtggc gatggtcgat catatcgtcg
     3481 tggcgatggg tgcggtcgtc gtcgcaatgg ctgtaatgat ttctgccagc aaactcttga
     3541 ccgaatttgt cgacagacac cctaccgtcg tgatgctctg ccttggtttt ttgttgatga
     3601 tcggtttcag cctgattgcc gaagccttcc atttccacat tcccaaaggc tacctctacg
     3661 ccgccatcgg cttctcgatt ttaatcgaac tgtttaacca gatttcccag cgcaacagcc
     3721 gcaaaaacga ctacatcggc agctcgtggc gcaagcgcac cgccgaaaac gttttgggta
     3781 tgatgggcat acgcgaaagc gtgcttgccg atgcgggcgg cgaatccggg gacgacgcgc
     3841 attttgaaga aaacgaaaaa tcgatgatac gcagcgtgct gacgcttgcc gaacgcccga
     3901 ttatgggggt gatgatccca cgccgcgaca tcgaacggct ggacatttcc caaagccgcg
     3961 aagaacagtg tgcccaactg caaaacacgc cttacagccg cctgctcgtt gtcggtaagg
     4021 cgggcgtgga cgaacctttg ggctacatca acaaaaaaga cctgctgtcc caactgctgg
     4081 aaacaggcgg tctcgacatt cagacggcat tgcgccagcc actcgtcctg cccgacagca
     4141 ccaccgcgct gggcgcaatc gaactcttcc gccaaagcag cgcggattat gctttggtgg
     4201 tggacgagtt cggcgcggta ttgggcatgg taaccatgaa agacctgctc gaaaccatcg
     4261 caggcgagtt ccccgaagaa tttgagcgcg aagaagaacc agccgttcag gggaatcccg
     4321 atgaaagcct gacggtggaa ggcgcgttgg aatatgtgga actcgcaccg caactcaacc
     4381 tgccgcagca ggaggaagat gccgatttcc atacggttgc cgggctgatt atggaagaat
     4441 tgcaaaccat ccccgatgtc ggcgattttg ccgatttcca cggctggcgg tttgaagtgg
     4501 tcgaaaaaga agggcagcgc atcgagcggg tcaaaatcac caaattgccc gaagaataag
     4561 tattcaggat agaaaatgaa cgttttgatt tccaacgacg acggctacct ctccgaaggc
     4621 atcgccgttt tggcgcgcgt tacggcggaa tttgccaacg tcagggtggt cgcgcccgaa
     4681 cgcgacagga gcggggtcag caattcgctg acactggaac gccctttgca gttgaaacag
     4741 gcgcaaaacg gcttttacta tgtcaacggc acgccgaccg actgtatcca catcgggcag
     4801 tctgtatttt cggattttca ggccgatttt gtcttttcgg gcatcaaccg gggcgcgaat
     4861 atgggggacg acacgcttta ttcggggacg gttgcggcgg caaccgaagc ctaccttatg
     4921 ggcatacccg ctgtggcgtt ttccttaaac gacgcttccg gacgctattg ggcaaccgca
     4981 gaaaaggcac tgtggacatt gttggcgcat tttttcaaaa accccccgca gtcccctatt
     5041 ttgtggaaca tcaatatccc cgccgttgcg ccggaagatg tgcggggcat taaaatcgcc
     5101 cgtttgggca ggaggcatca cggtcagaac gtcatccccg cgcgcaatcc gcgcggcgaa
     5161 cagatttatt ggataggacc ggtcggcgaa gtttccgatc gggaagaggg aacggatttc
     5221 ggtgaatgcg gcgcaggttt cattaccgta acgccgctgc aaatcgacct gaccgcctat
     5281 ccggacatgg cggaaacagc ggcgttctgg catgcggact gaccgtttca tcaaatatag
     5341 tggattaaca aaaaccagta cggcgttgcc tcgccttagc tcaaagagaa cgattctcta
     5401 aggtgctgaa gcaccaagtg aatcggttcc gtactatttg tactgtctgc ggcttcgtcg
     5461 ccttgtcctg atttttgtta atccactata ccgtcgggct ttgcgcttga gaagaacgcg
     5521 catttttatt atgatatacc gcattcaggc tatatatcgg aacatgaagg ttatcttatg
     5581 ttgaaacaaa cgacactttt ggcggcttgc gcctcctttg ccgcactgtt gggcggttgt
     5641 gctacccagc agcctgcccc cgtcattgca ggcaattcag gtatgcagga cgcgccgtct
     5701 tcggcagttt acaacaaccc ctatggagca acgccgtaca gcccggctcc tgccggcgat
     5761 gcgccttatg tgccgccggt gcaaagcgcg ccggtttata cgcctcctgc ttatgttccg
     5821 ccgtctgcac ctgccgtttc gggtacatac gttccttctt acgcacccgt cgacatcaac
     5881 gcggcgacgc atactattgt gcgcggcgac acggtgtaca acatttccaa acgctaccat
     5941 atctctcaag acgatttccg tgcgtggaac ggcatgaccg acaatacgtt gagcatcggt
     6001 cagattgtta aagtcaaacc ggcaggatat gccgcaccga aagccgcagc cgtaaaaagc
     6061 aggcccgccg taccggctgc cgcgcaaccg cccgtacagt ccgcacccgt cgacatcaac
     6121 gcggcgacgc atactattgt gcgcggcgac acggtgtaca acatttccaa acgctaccat
     6181 atctctcaag acgatttccg tgcgtggaac ggcatgaccg acaatatgtt gagcatcggt
     6241 cagattgtta aagtcaaacc ggcaggatat gccgcaccga aaacctcagc cgtagaaagc
     6301 aggcccgccg taccggctgc cgtgcaaacc cctgtgaaac ccgccgcgca accgcctgtg
     6361 cagtccgcgc cgcaacctgc cgcgcccgct gcggaaaata aagcggttcc cgcgcccgcc
     6421 ccgcaatctc ctgccgcttc gccttccggc acgcgttcgg tcggcggcat tgtttggcag
     6481 cgtccgacgc aaggtaaagt ggttgccgat ttcggcggca acaacaaggg tgtcgatatt
     6541 gccggtaatg cgggacagcc cgttttggcg gcggctgacg gcaaagtggt ttatgcaggt
     6601 tccggtttga ggggatacgg caatctggtc atcatccagc ataattcttc cttcctgacc
     6661 gcatacgggc acaaccaaaa attgctggtc ggcgaaggcc agcaggtcaa acgcgggcag
     6721 caggtcgctt tgatgggcaa taccgacgct tccagaacgc agcttcattt cgaggtgcgg
     6781 caaaacggca aaccggttaa tccgaacagc tatatcgcgt tctgactttc tgcctgaaat
     6841 caagttggaa atgccgtctg aaaggttttc agacggcatt tttgtttttg gcggctgtgt
     6901 tttcaaatca ttcggaaggg atggggcata taaatttgtt tgactgtgtt tgg
//
LOCUS       DAWHRA010000109         6763 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWHRA010000109 DAWHRA010000000
VERSION     DAWHRA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.109"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1264)
                     /locus_tag="WIB30_001641"
     CDS             complement(<1..1264)
                     /locus_tag="WIB30_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ3797679.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAEGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRG"
     gene            1341..2309
                     /locus_tag="WIB30_001642"
     CDS             1341..2309
                     /locus_tag="WIB30_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797680.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWGGTNGGTQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(2510..3976)
                     /locus_tag="WIB30_001643"
     CDS             complement(2510..3976)
                     /locus_tag="WIB30_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ3797681.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4218..6329)
                     /locus_tag="WIB30_001644"
                     /pseudo
     CDS             complement(4218..6329)
                     /locus_tag="WIB30_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1444 a         2007 c         1728 g         1584 t
ORIGIN      
        1 tgccgcgcaa gcgcgcaagc agcgcgtccg ccttctgttt ctgcgcggca aacgccgcct
       61 tgtccgcctg cgtcaattcc ggtgtcggca atgcgaccga aacaggattg acgggctgcc
      121 cgttgatgcg cgcctcgtaa tgcaggtgcg gcccggtcga acgcccggtc gaaccgacaa
      181 aaccgatgac ctcgccgccg cgcacattgc cttccgcctg cgaaaacgcg ctcaagtgcg
      241 cgtacagcgt ttccacaccg ttggcgtggc gtatcatcac cgcgttgccg tatccgccct
      301 tccggccttt aaaggtaatc acgccgtcgg cggaagccct gaccggcgtt ccctgcggtg
      361 cggcataatc gatgcctgtg tgcagccgcc atgtgtgcag gatggggtgc atacggtagc
      421 cgaacggcga agaaatgcgc gtatagacca gcggctcgat gttgaagccg cctttttcct
      481 gcaacaccct gccgtcttca tcataataat tgccgccccc tccgccttcc ttgtccgaac
      541 ggtaatagaa tgcctgatgc cttgtgccgc ccttaacgac ttcagccgcc aaaatatcgc
      601 ccgccgccac ctgctgcccg tggaaataca ggctgtcgta catcaggcgc acggcatcgc
      661 cttccttcaa accgtcaagg ctgaagcggc cggcgaaaat cccgcttaag gattcgcgga
      721 tttcgacggg cacttccgcc cgcgccagcg aaccgcgcgc cgacgttttg accacgaccg
      781 aacgcagcgt cggcaaaacc ttcatatccg cctcagaagc cgaccgccgc catatgccgc
      841 ctttcttttc caaagcgacc agattgcgct cgccgtcttc gtcggtaaaa aactgtactt
      901 cgcgcgcgcc gccgtcgccg ccgaccaaaa catgaaccga ctggtcggca cgcaaatgcc
      961 gcaaatcggc ttcgccgcca tatttttccg tgattcgggc aatctcgtcc cgcgccatac
     1021 ccgaacgcgc cagcacgtcc gccagcgagt cgcccggctg caccgcctcc tgcacccaat
     1081 atgccgtctg aacgccgctg ccgccccaag acagcggcgg cagattttgt tccacgcgct
     1141 gcggtctgac gcgttccgtc ctctctgtca aagcaatgta tgccgccgac accaaaataa
     1201 tcgaaacggc aagcgcgcgc agcgcgtatt tccgatgttt tgccgaaagt gggaagacag
     1261 ccatattgtt cctttttgac ggcgtttgcg ccgaatcctc tataatatcc gcccgatccg
     1321 catttgtcag acatcaaacg atgaaactca ccctcgccct cccctcgctc aatttggatg
     1381 aagacgaaat ccgaatacct ctatgcctgc ccgccttcaa caaaatcctg caatacggct
     1441 cgctgcaccg ccaaagctgc acggcttcgg cattttacgc gcgttatttg tggtgcggca
     1501 gactggcgga acgcgcggcg caaagcctga atatgccgtc tgaaaccgtc gcgctcgcca
     1561 cgcccgtctg gcaaaagatg gggatgcatc aggcaaatgt gctgacggcg gaatatttgg
     1621 atgtcggtac ggatgaagcg gaacggctct gccgcgatct gtcggcgttt tacggcgata
     1681 ttccgtggcg tttcgttccc gtcctgcccg aattatggct ggtttccctt ccccgcgcgt
     1741 accgctgggg cgcgaagccc gttttggact tgggcggatt gttgggcgcg gacgatcagc
     1801 cggacggaga agatgcgttg gaatggctgc gcgtccaaac cgaaatccaa atgtggctgg
     1861 cggcgcaccc cgtcaaccat aaccgcaaga aacgcgggct gcccgagttg aacggcttat
     1921 ggctttgggg cggcacaaac ggcggcacgc aaggcggcac gcttttcgcc gatacggttt
     1981 ggagccgttt ccaccccgac cgccgcgcat tgcccgacaa cttccgcgcc tatgaagaaa
     2041 cggcggcaca cctgcccgac acgcaccata tcctgtttat ggacgatttg cgcctgaccg
     2101 ccctgacagg ggacagggag cggtatgcgg caatattgca gcagtgggaa gaacgctggt
     2161 tcgcaccgct ttacgaagcc gtccgcatcg gcaaaatcaa acggctggac atcgccacgg
     2221 acggacaaca cggcggtacg ctgaccttca aacccgccga caggtggaaa ttttggcgcg
     2281 gcgcgaaaac ctttgacgga atctggtgaa cccgcccgcc gcaaccgcga caatgccgtc
     2341 tgaagcccga atcgggtttc agacggcatt ttacctcgca tcgccgttat gccggaaaaa
     2401 ccggaatgcg tttccgcaca cgcggcggca cgcaaagcac cgcccgaaag ccgccgatgc
     2461 ccgccgtgat accgccgccg gcagggtttc ctccgaaaag gaacagcaat cagaacgttt
     2521 tattaaactc gacaaacgcc cgatttttct cgtattcgtt gaacacatcg ttactccgcg
     2581 tttcgcggtg cgacaacgtc aggcgcggcg tgatgccttt gaaatgcaat gcccggtgcc
     2641 aaaggctcaa ggatgtgttc aattctttat ccctgcgcct ttccccttta aaaccgctga
     2701 aaaagccggg tttttcataa tgccgtttcg ccgcgccgag gcgcaacagc gaagacaggc
     2761 cgctgccgcc ccattcctgc ccccaggcaa agcgcaggcc gtaacggttg aaattgtcgc
     2821 cccggtcggc ggggttgcgc tcgcggtaaa aatccaaacc gcccatccaa tattggcgcg
     2881 cattccggta aaacaccagc gaattggaaa tttgcaaatg ggtattgtcg gaacgcgccc
     2941 ggcgcgtatt cttcaaacgc ccccactccg ccgaagacaa cgtttgccat ttcggggttt
     3001 gccaacggtt gaaataaagg cgtgcgccgt tggtgtaaga ataagcgtcg ttgccgtagg
     3061 tgcggcgttc gtggaacact gccagcccgg catctttgcg ccggtcggca aaaccgatgc
     3121 cgccggaaac gcctgccgtc atatcgttga atttcttatt ccccggataa accctgccgg
     3181 acacgtcgcc gcccgccgtc gtgtaccagc cgtttttcag cgaccatttt ttctccgcgc
     3241 cgagccggta attgaccgcc gtgccgtcca cctgtttcgg gaaagtccat ttgccgtact
     3301 gctgccgttt cggggcttgg ttgatattgt gttcgcgggt gacgctgaag ccgccattta
     3361 ccttccacgc atcgcgttcg cgcaatgcct tgcggtacag ctcgacctgc tccatcagct
     3421 gcggcggcag gttttccgcc ttcaggcggt cgaactggtc tgccgccgcc tcgttctgcc
     3481 tgttttcaaa caatgccgcc gccaaacgca tacggacggc gggcgcgtcg ggttgggcgg
     3541 caatcaattc ccggtaatgg gaaatcgcct ccttcaccct accgtctgcc tgcgccaaaa
     3601 tcccttgtgc ataaagtgcc aacatcttat cctgctgcgc ctgttgtagg taaatcggca
     3661 aaataacgcg gataccggca atattgtttg agaccactgc ggaatacatc gcgcgggaca
     3721 acaattcggg atttttcagc agggtttctc cgtcaatctg caaaactttt cctttttccc
     3781 gcacctgccc cggtaccttc tccctgtcga ttggtttgac ctccgcttca tgaagcctga
     3841 actcgggacg gcttctcaaa tccggttcgc gcggtgtttc ttcggcatat gccgcgcttg
     3901 ccaataaagg cagcagcagc atccatctgt ttcgtgcagg cataaatgtc ttcccacaaa
     3961 aataaaaaat aaccattatc aatcggcgga ttgtatcagt tttttatgcc gctgttccaa
     4021 accctgccgc ccgatgccgc gacaatgccg tctgaagcca gaatcgggtt tcaaacggcg
     4081 gcaaaaaacc ggcggttttc cgccggtttc atcatgatgc cccgcccttc ggggcggcag
     4141 ggttcggatt ggtttgcggg gcggcgcgcg gggccgtgcc ggtgtgccgc ctgtgcgtca
     4201 aaacatttcc cgacgcatca aaacagccat tggagcgaca atgcggcttc tttgtcgccg
     4261 tccgtccttt tgccgtatcc gatgcgtgcg gacatatggc ctttccaacc ggcttcaatg
     4321 ccgaaccgcc cttcgagcgc cgtcctgcct gccagcgtct gtttttcgcc gtccatttcc
     4381 acgccgaaag attttgacct gtgcaaaaca ttaaaagcgg caaaaggctg aagattgaca
     4441 ccgttacgca aagcaaaacg ggtttttgcc cgaatgccag cgcggctttg ccactgaccg
     4501 ctgccgagca gtccgaccgc cgtcccctcg ctgtcggtaa agccgccgtt tacgcccaag
     4561 taggtaaact gagcctgcgg ttgcaggtaa aaccgcacat tattgccttt tccgacaacg
     4621 ccttccgcca caagcgcgtt gtagccgcct tcgacagaag ccgtccaacc tttggttttg
     4681 tagcgttccg cacggttttc atcattgatg cgatgtttga aacgttggta ttgcaaccag
     4741 ccgtccaaat acgcacccgt ttgtttatcg cgcaactgat gccacgcagc ataaacaccc
     4801 ccgccataac catgcaaata actgcctgcc gcaccgcctt tgccgttgac tgatgcgtgc
     4861 tggccggccc tgccgcccat cacgccgatt gccagtcggc tgccttcatt ttgccgtaca
     4921 aacacctcgc cgccgatttg cacgcctttg cgccgcccgt ccgcagccgc gccgccccgt
     4981 atattttgat gcgaccgacc gccgatgaag cgcagccaca atttttgacg ggacggatcg
     5041 gcggcataca cgtcgtcgcc cctgtcggcg gcacgcagcc caaacaaagt atttgccgca
     5101 taagcctgtt gggcatataa aaccgcttcc gcctcgggga tagccaaacg ctgcacgccc
     5161 aagcaaaatc cgccttcgca ttgcttgagc aataattcgt acagtccctg ccggtaattt
     5221 tgtttaaacg caaacgaatt agggtctgcc ttttccgccg caatcagttt gcgcccgtcg
     5281 agcgttttca gattttccgg ccggcgttcc aaaaccacat tggattttcc gctgacgttt
     5341 tgttcaaagg tcagggcggg ttcgtccgac tcccgcacgt cgtagccgaa caggaaatcc
     5401 gcgccgccct gatacgcttt atggacggtc agtttttcat ctttcgtgtt caggcggatg
     5461 cgggcattat cggaagtttc cagcgtatgg atgtcggaat ggcggcgcgg ttcccaaagg
     5521 gaatctttca gcgtcatctt ggtcagttcc aacacctgtc cggcgacgtg ggatttgttt
     5581 tcgattttga cgtgtaattt tttattgaag atgttgttga gactagtctg tgatgtatta
     5641 ctatgaggag tgagtacgga atcttctacc aatttattaa ttaaatcggg catcgtctgt
     5701 ttgagcgtac taaattttgt tccaaactgt gctatatacg cctcctcagt ccgtttttta
     5761 ttttctgccc aagcttccgg tcttgttttg tataaatcct gtaattgact tttaaccgcc
     5821 tgctttaaat cagccccata cttctctgta atatattttt tgacaacggg catattatcg
     5881 acggcgagga tgccgctatt gtaattttta ccttggatat tgatttcgtc atacccatat
     5941 tcggatgaat tttgatcggt tttaccgctg gaaaaataat gggaagtgcc gggtttcggc
     6001 ttgtctgaaa atgaaaaatc ggcatcgtcg ccgattttgg tagaacgagt atttgaacca
     6061 agctgagctc tgaacatacc gaagcaatca aaagatatgc tgcacgggcc gcgccaaacg
     6121 tgggaatttc tgtcttccgc cgcttctaaa ataaatttgt ttttaatctc ggcttctttg
     6181 acgggatcgt acgcatccga acctcaaaca tatggcgtgc aaaacagcga taaaacagat
     6241 aatgttaata gattaacccg accgcttgct tgcttgcttg cttgcttgct tgcttgcttg
     6301 cttgcttgct tgcttgcttt cagtgtcatg agaaattttc ctttgtcaag tgttaaaatt
     6361 ataatgatta tatactacat tatactacat cgcaatgaaa aatcaggaaa agcaaaaaac
     6421 cctccgccgt cattcccgcg aaagcgggaa tctagaattt taatgccgca agaatttatc
     6481 aaggatggtc ggggtttaaa ggtctggatt cccgcctgcg cgggaatgac ggtttagaaa
     6541 tcacccgaaa caagaaaaag aaccgaaacc gaacaagccg gattcccgcc tgcgcgggaa
     6601 tgacgggatt tcaggttact gtttttgatt ttctgttttg tggaaataac gggattttgg
     6661 atgatgtgca tttatcgaga aaactaaaaa ccctccgccg tcattcccgc gaaagcggga
     6721 atctagacct ttcggtgcgg aaacttatcg gataaaacgg ttt
//
LOCUS       DAWHRA010000110         6752 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWHRA010000110 DAWHRA010000000
VERSION     DAWHRA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.110"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            244..528
                     /locus_tag="WIB30_001645"
     CDS             244..528
                     /locus_tag="WIB30_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ3797682.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     NSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            529..1716
                     /locus_tag="WIB30_001646"
     CDS             529..1716
                     /locus_tag="WIB30_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ3797683.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMAGDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYALPVIILLIPL
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGEPVNNGNLIADMFVPTLTE
                     KPESKPIYNGVRQVKTFEYIAGCVQGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVRQSAQQHSDRAQVATLGGKPLQNLMYDNWQERGKPFEGIGGGVVG
                     SAN"
     gene            1931..2887
                     /locus_tag="WIB30_001647"
     CDS             1931..2887
                     /locus_tag="WIB30_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ3797684.1"
                     /translation="MNIIGLDISKDTIDATLHKINGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
     gene            complement(3254..3487)
                     /locus_tag="WIB30_001648"
     CDS             complement(3254..3487)
                     /locus_tag="WIB30_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797685.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFLSFSHFR"
     gene            complement(3517..6282)
                     /locus_tag="WIB30_001649"
     CDS             complement(3517..6282)
                     /locus_tag="WIB30_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ3797686.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHYSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQDGQLKFNSDSGKWER
                     DLQRQQWKYPWYKKYENPQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTRDVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     ITMSYKF"
BASE COUNT         1901 a         1518 c         1610 g         1723 t
ORIGIN      
        1 gggggtgtgg gggactagtc ccccgcaaag cgttcagctt cggaaacttt ggccgaaagg
       61 caggcgaagc agcgcacttt gcgacgaatg tcgcaaatag ccgagaagcg cggggggatt
      121 ggcgataagc gcgagggggg tgtccccaca gcgccgccgc gccgcgaatg cggcgcaaaa
      181 tctttcagat taagaaacat ttgtttaatg aggcaaccgt gccttttaag aaagggatag
      241 caaatgaaat tgttggccgc attgattccg cttttgatga gcgtggcagg ccgtatattg
      301 actgcattag gcttgatggc ggtaacctat tcaggggtgg atagattggt agcccatttt
      361 cagcaggcga taaccaatag cataacgggc gcgcctcaag cgatgttgca gcttttttat
      421 ataagcggcg gtggaaccgt tcttaatatc ctgtttggtg cgatcgcctt tattctgtca
      481 tttaaacaaa tgacaaaact agcaacctca atcgggaaga aaaaataaat ggcagagatc
      541 tgtttgataa caggcacgcc tggttcaggg aaaacattaa aaatggtttc catgatggca
      601 ggcgatgaaa tgtttaagcc tgatgaaaac ggcatacgcc gtaaagtatt tacgaacata
      661 aagggcttga aaataccgca cacctacata gaaacggacg cgaaaaagct gccgaaatcg
      721 acagatgagc agctttcggc gcatgatatg tacgaatgga taaagaagcc cgaaaatatc
      781 gggtctattg tcattgtaga tgaagctcaa gacgtatggc cggcacgctc ggcaggttca
      841 aaaatccctg aaaatgtcca atggctgaat acgcacagac atcagggcat tgatatattt
      901 gttttgactc aaggccctaa gcttctagat caaaatctta gaacgcttgt acggaaacat
      961 taccacatcg cttcaaacaa gatgggtatg cgtacgcttt tagaatggaa aatatgcgcg
     1021 gacgatcccg taaaaatggc atcaagcgca ttctccagta tctatacact ggataaaaaa
     1081 gtttatgact tgtacgaatc agcggaaatt cacacggtaa acaaagtcaa gcgttcaaaa
     1141 tggttttatg cattgcccgt catcatatta ttgattccgc tatttgtcgg tttgtcttac
     1201 aaaatgttga gcagttacgg aaaaaaacag gaagaacccg cagcacaaga atcggcggca
     1261 acagaacagc aggcagtact tccggataaa acagaaggcg agccggtaaa taacggcaac
     1321 cttatcgcag atatgtttgt cccgacattg acggaaaaac ccgaaagcaa gccgatttat
     1381 aacggtgtaa ggcaggtaaa aacctttgaa tatatagcag gttgtgtaca aggcggaaga
     1441 accggatgca cctgctattc gcatcaaggg acagcattga aagaagtgac ggagttgatg
     1501 tgcaaggact atgtaaaaaa cggcttgccg tttaacccat acaaagaaga aagccaaggg
     1561 caggaagttc ggcaaagtgc gcagcaacat tcggacaggg cgcaagttgc cacgttgggc
     1621 ggaaagccgc tgcaaaatct tatgtatgat aattggcagg agcgaggaaa accgtttgaa
     1681 ggaatcggcg ggggcgtggt cggatcggca aactgaagaa aacggcaaga gagaaaaaag
     1741 acccataaac cgtttgaata tagacggctt acgggtcttt gtttcgcgca aagcaaaggc
     1801 taaggcagtc aggcagcaaa tcccgcaatg tatcaaaaca gacgcgtaga aatgccggct
     1861 gcctttatcc atcctcaaaa ttgaatatca tcctagccgt atcaaggctg tataaataag
     1921 gaaaatacca atgaatataa tcgggctgga catctcaaag gacaccatag acgcaacatt
     1981 gcataaaata aacggaagta tccattacat taaatttaag aataatgatg atggattaaa
     2041 acagtttaga ttgtggataa agggaaacag aatcagaaaa gcctatatcg gcatggaggc
     2101 aacaggcatc tattacgaaa aggcagcaga tatgctttct tcctactata ccgtttacgt
     2161 tatcaatccc ttaaaaatca aggactacgg aaaaagcagg tttaaccgta ccaaaaccga
     2221 caaagcagat tcaaatctga tagcagatta cataaaaagg catcaagata cattgatacc
     2281 gtatcagata cccaaaaaca aagcactgca aaaactgatt aatcttaaaa accaattaca
     2341 gcaacagcag aagcaaatta aaaatcgtct ccatagcact gaagaagact tcataaggaa
     2401 catacatcaa gacttgatag ataccataca ggacaagatg gaacaggtaa aaatagccat
     2461 atccgaacaa atcaaaaaac aaacggacaa taaccattac cgcaatcttc aaaccatccc
     2521 gagcataggc aaagacaccg catcagttct ttatgcgcaa ctgacagaaa aacattttaa
     2581 aaccgcaaac cagtttgtat cctatgccgg attaagtccc gccatcatac aatcagggac
     2641 aagcgtaaga ggtcggggca gattgagccg atacggaaac agacgattaa aaagtacgct
     2701 gtatatgccc gccctttgtg cttaccgttt taacgcattt ccgaaattaa taaataatct
     2761 gaaaaaagcg ggtaagccaa agatggtaat catcgttgcc atcatgcgca aactggcgaa
     2821 gctcgcctat tacattgtta aaaccggcca gccttacgat gcggaaagac accgattgaa
     2881 tcaataaaat tcaacaaaat taaacggtta cgcgaatata tttgtgtaac cgtgcatttg
     2941 catatcgtaa ataaacgtaa ataaaaataa caatataaat cagtatattg caactttgtt
     3001 ttttattttg tgttgacggg caacatatca tctgcgcggg aatgacgaat ccatccgtac
     3061 ggaaacctgc actacgtcat tcccacgaaa gagggaatcc agttttttga gtttcagtca
     3121 tttccgataa attgccttag cgttgaatgt ctaaattccc gcctgcgcgg gaatgacgaa
     3181 tccatccgta cggaaacctg caccacgtca ttctcacgaa agtgggaatc cggaaagcta
     3241 aagccacagg aatttatcgg aaatggctga aacttaaaaa aactgattcc cgcttttgcg
     3301 ggaatgacga ctcataaatt acccgaaaca accaaaaata accgaaatca aaaaccggct
     3361 tccctgcgtg ggaatgaaat ggatttttct ttgagttgcc gatgcgataa aacggattgg
     3421 cataggcttg cggcaggatg atgcgtttgg ggaaacaaat gccgtctgaa gggctttcag
     3481 acggcattgc ggttttccac aaaacgcggg ctgcctttaa aacttgtagc tcatcgttat
     3541 caaaaaggtg cgtccgcgtg caaaattggt caatacgctt ttgcttgtgc cgccgtattt
     3601 gccgttgcac aagccatcag cattacacgt tacgtcttcg tccttgtctt tcgggtcgaa
     3661 cgagctgtaa taacgctgcg ttgccgcatc attgcccgca tcgagcggat cgatataacg
     3721 cctgtcgaac agatttttga cttcggcgcg gaaaataagg tttttcttcg gctcgtaagc
     3781 ggcgtaaaaa tcaaaaatca aaggctggcg ggcaagggtt tcggtttgtt tgatggaacg
     3841 cttgcccagt tgccggacat cgcgggtatt tcccccgttg gtgccgtcga tatagcgttc
     3901 ttcagccgtc gcgcggatgc tcttgccgaa atagcgcatc gcgccgccca aagtcagctt
     3961 gttgcccaac cagcgcgtac cgacttccaa acgtccgtaa tctcgcggca gggcggaaac
     4021 cctgctcaac ccataacctt gtttgagttg gtcttctttg gacgcattgt tgggcgattc
     4081 gctcgcatcg ctgaagttgg tcggttgcgt gcttttttga taggcgtaag aaaggttggt
     4141 gaaaaaacgc ccataatcgt aattcagctc caactcaaaa ccgtgtttgt gcactttgtc
     4201 tttgaaattg cgatgttgga tggtgtaggc aagcccggtg ctgctgaccc agctcggaat
     4261 atccccgttc aaatcccacc atttcccgta aacgttgtgg atgtagttgt cgatgcggct
     4321 gcggtagccg accagtttta atcctaatgt atcatcttgt tttaacagtc cttttttata
     4381 ggtattgaag ccaaattgcc aagtgtttgc gcgctctggt tttaaggcgg tgtgaacgcc
     4441 ggagtcgccg atttgggaaa aatacatttc ttggatgttg ggcatacggt gtgtgcgcga
     4501 atagctggcg aacggcatga aataatcgcc gaagtccgca ctaatgctga ccgaatggtt
     4561 gttggcgcgc tttttgccgt attttttcaa tacgggttca taaagcccgc agctcgggtt
     4621 gcaatgtttc ttgtatgtcg gcgagttttc tccgaatgcc cgcttaaatt cgccttccga
     4681 gctgtaatag cccgtatatt cgccgccgaa acggtagccg acggtattgg tgctgtagtt
     4741 taagcggtaa atgtcttttt tgagcgcggc atcgaagtag aacgtgttga aatattggct
     4801 gccggccggt tggacaatgg ttgatttttg gggcagcagc cctttatcgc ccttaaaccg
     4861 ccccaaatag gaataaagcc cgttgtcctg atcaggaccg tcgaaaaaca gccccaattc
     4921 ttcaggaaag cggtttttgc cgtattcgtt gtggaaataa ttgaagccca aagtggtttg
     4981 caactcggtt tcgcggggca gccggaaggt ggcggtgttg ttgaggtcga ggatttttgc
     5041 gttgttgtag gtttcaaaat cctttaaaag cccccagcct gtaaacttcg accctttcgg
     5101 atatttctgc ctgcccgaat tgtaggctgc ggtcagattg aggttggtat acgagttcaa
     5161 agacaaaccg taattgaatt gataattgcg gttgatgatt ttgcggctgc cgattttggt
     5221 gtttaaatcg cgaaattgcg ccgtgtattt attgaatacg ccgtcgtatt ccaatttaaa
     5281 cagattacct gccgactgct gcttcaggct ggacggatcg atgggggtga tgtcgtattg
     5341 cggcgccagg ttttcccgcc agcttttgtc atgctcttcg atgtattttt gcagttcttg
     5401 ggggttttcg tattttttat accacgggta tttccactgt tgtctttgca agtcccgctc
     5461 ccattttccg ctgtcggaat tgaatttcaa ctggccgtct tgtacaaaat atcgttgctt
     5521 gcgccgttcc agatattccg cgccaaaatt tccgatgtgc tgcccgccgc cgcccacgcg
     5581 gtaattttgc gccacgctgc gcctgctgtg cccgtaaagc acaccgacag atgctccgct
     5641 ttccagccat ttgcgcgcac ctatcgccgc catcgcatta cctttggttg aattggtgcc
     5701 ggtcagacct tttagcagca ggccgtaggt attattgccc tgaacgacgt catccacgcc
     5761 taaagtccgc agattcgccg aaccggcaag gctgttgatg cctgccgagc cgctgaagct
     5821 gcctttgacg acatccagtc cggcaataaa attgctgtcg acagatgcac cgaattgaga
     5881 tgaaccgcct gccctgcccg catcggtaga agtcgaataa aaggtctgcg tgatgccgtc
     5941 caccatcgta ttgacccgcc cgaacccgct gtcgccgcga atattcaaag acacaatgcc
     6001 cgagctttta tcttgctgtg taaacgcacc ggggatgctg cgtacgatgt tgtcgaggtt
     6061 ttcgctggat ttgaatatat cctgacgggt cgatacggca cgcgcatcgg taaacacttt
     6121 tttgtctttc ggtacgcgct tcgccttgac gtgcacatct tccaaaacct gtatctgcgc
     6181 ctcgctgccc gcgcgccctg catcttcggc ataactataa tgatatagca taacacccat
     6241 aaggtaaaaa caaatcggct tcaaccggaa agaagatctc atattttcct caacaataaa
     6301 cagtcagaca attaggaaat atactagcat ttttttcagg cggcataaac attaaaaaag
     6361 tgtaaatttg atataccgtc tgaagatttc aattggatat ttatagtgga ttaacaaaaa
     6421 ccagtacagc gttgtctcgc tttagctcaa agagaacgat tctctaaggt gctgaagcac
     6481 caagtgaatc ggttccgtac tgtttgtact gtctgtggct cgccgccttg tcctgatttt
     6541 tattaatccg ctataaagac cgtcgggcat ctgcagccgt cattcccgcg caggcgggaa
     6601 tctagtcggt tcggtttcag ttatttccga taaatgcctg ttgcttttca tttctagatt
     6661 cccactttcg tgggaatgac ggttcagttg ctacggttac tgtcaagttt cggttatgtt
     6721 ggaatttcgg gaaacttatg aatcgtcatt cc
//
LOCUS       DAWHRA010000111         6682 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWHRA010000111 DAWHRA010000000
VERSION     DAWHRA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.111"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..892)
                     /locus_tag="WIB30_001650"
     CDS             complement(<1..892)
                     /locus_tag="WIB30_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HEZ3797687.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHLPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIV"
     gene            complement(978..1421)
                     /gene="secB"
                     /locus_tag="WIB30_001651"
     CDS             complement(978..1421)
                     /gene="secB"
                     /locus_tag="WIB30_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ3797688.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(1442..1699)
                     /gene="grxC"
                     /locus_tag="WIB30_001652"
     CDS             complement(1442..1699)
                     /gene="grxC"
                     /locus_tag="WIB30_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ3797689.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHTAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            1913..3406
                     /gene="rng"
                     /locus_tag="WIB30_001653"
     CDS             1913..3406
                     /gene="rng"
                     /locus_tag="WIB30_001653"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ3797690.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(3441..4691)
                     /locus_tag="WIB30_001654"
     CDS             complement(3441..4691)
                     /locus_tag="WIB30_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ3797691.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERVRVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(4687..4862)
                     /locus_tag="WIB30_001655"
                     /pseudo
     CDS             complement(4687..4862)
                     /locus_tag="WIB30_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(5080..6393)
                     /locus_tag="WIB30_001656"
     CDS             complement(5080..6393)
                     /locus_tag="WIB30_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ3797692.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1618 a         2005 c         1687 g         1372 t
ORIGIN      
        1 ccacaatggt tttgccgctg ccgacatcgc cctgcagcag gcggtgcatc gggtaggttt
       61 gcgccatatc gcggcagatt tcggaaacaa ctttttcttg cgcatcggtc agggcaaacg
      121 gcagggcttg gcgcagggct tgggtcaatg tgccgtcgcc gcccaatgcc gccgccgtgc
      181 cgccgatacg cttctgtcgc gccaagcgca tcgaaagctg ttgcgccaaa agttcatcga
      241 atttgagccg ttgccatgca ggcagcgtgc cgtctgaaag ctgatgaatc gtgaaactcg
      301 gcggcggcaa atgcaaaaga cgcaggcttt cggcgaggtg tggcagcttc agacggcaca
      361 gcagggcatc gggcagcgtg tcgtgcagcg gcgtaacgtc caacgccgtc tgaataatac
      421 ggcgcaaagt gggctggttc aaaccgttta cggtcgggta aaccggcgtg aggctttccg
      481 ccaaaccgcc gccctcggca tcgcggattt tgggatgaat catctcgtcg ccgtaaaagc
      541 cgtgtttgat ttcgcccacg gcgcggatgc gtttgccgac cgccgtctgt ttctgatggc
      601 tggcgtaaaa gtggatgaag cgcagaaaaa ggacgctgcc ggaaccgtcg gcgatttgga
      661 caatcaactg cttgcgcggt ttgaacgtta cttcctgatg gataacctcg ccctcgacct
      721 ggcacggcac gccaatcggc gcgtccttaa tcggcataat gtgcgtctcg tcctcgtaac
      781 gcagcggcag gtgcaacacc aaatcccacg cggtatggag gttgagtttg tcgagcttct
      841 tggcggaaac atcggtgatt ttgagctgtt tctgggtttc gggcgacatc ataggcagat
      901 tcctttgaac gcacctattt tatccgaaaa caaaaatacc gtctgaaacg gattcagacg
      961 gcatcggcag gcaggaatca agccccggcg gcttcggctt cctgctgttg ttggtaaatc
     1021 gcctcaaaat taatcggcgc gagcaggacg ggcgggaagc cggcacgtgt taccgtaccg
     1081 gaaacggctt cgcgcgcgta agggaagagg atgttcggac acgccacgcc cagcagcagc
     1141 tgcacatctt cttcggggat gttttccaaa cggaagatgc cactttgggt tacttcgttc
     1201 aaaaacatcg tgcgctcgtt atccagcttg gcggtaacgg ttacggtaac gtccacgttg
     1261 tagtagccgt cttccagctt ttggctgccg gtggaaacgc gcatatccac ttcaggctcg
     1321 ccctgttcca aaaagatttg cggcgcgtgc ggcacttcca aagacaagtc tttgacatac
     1381 agtcgctcga tgctgaatac gggttgcagt tcttcgctca ttttgttttc ctagttgggg
     1441 gttaagggtt cagcagtccg tccagcccgc cttcctgctg gaggcggtag aggtcggtaa
     1501 atccgccgac gtgcgtttcg ccgatgaaaa tctgcggcac gctgcgctgt cccgaaagct
     1561 gctgcatttc ggcaaaggct tcggggcttg catcgacacg gatttcgtcg atatgtccga
     1621 cacctgccgt gtgcagcagc cttttcgcca tcgcgcagta gggacaaaac ggacctgtgt
     1681 acatggtaac ggtctgcata ttgggtttcc gaaagttttg caatgataat caatataggg
     1741 gcatttcccc tgtttggcaa gtgcggaaca gatgcacgtt caaacggcat gtgcggaatg
     1801 tgtcaaagtt tcttttttaa agtatgatag gcattgtgaa aaatattttt gcacccgcgc
     1861 tgcgcggcgg aacgggtgca aaaatatttt tattacattt tcaggaaaaa ccatgttgtc
     1921 aggactcccc atccccaaag acatcgcgcg cccgcccgaa acgatattgg tcaacatcac
     1981 gccgcaggaa acgcgcgtag cggtgttgga ggaaaacaat atctgcgagc tgcacatcga
     2041 gcgcaacagc gaacacagcc tggtcggcaa tatctatctg ggcgtggtgc gccgcgtgct
     2101 gcccgggatg cagagcgcgt ttatcgacat cggcttggaa cgcgcggcgt ttttacacat
     2161 cgtcgatgtc ctcgaacaac gccgcaaccc cgaagaaacc cagcgcatcg aacatatgct
     2221 gtttgaaggg cagtctgttt tggtgcaggt catcaaagac ccgatcaaca ccaaaggcgc
     2281 gcggctttcc acccaaatct cgctggcggg gcgtttcctc gtccatcttc cgcaagaaga
     2341 ccacatcggc gtgtcccaac gcatcgaaga cgatgccgaa cgcagcagcc tgcgcgaacg
     2401 cctcgacaag ctcctgccgg aaaatgcctg ccggggctac atcatccgca ccaacgccga
     2461 aaacgccacc gacgaacagc tccagtccga catcgactac ctgaccaaag tgtgggaaca
     2521 catccaagaa caggcgaaaa tccggccgcc ggaaaccctg ctttatcagg atttgccctt
     2581 gagtttgcgc gtattgcgcg atatggtcgg ctgcgacacg caaaaaatcc tcgtcgattc
     2641 caccgtaaac cacgggcgca tgacgcgttt tgccgaacaa tacgtccacg gcgcattggg
     2701 caggatagaa ttgttcaaag gcgaacgccc gctgtttgaa acccacaacg tcgaacagga
     2761 aatcagccgc gccctgcaac cgcgcgtcaa cctcaacttc ggcagctacc tgattatcga
     2821 atccaccgaa gccatgacca cgatagacgt gaacaccggc ggcttcgtcg gcgcacgcaa
     2881 cttcgacgaa accatcttcc gcaccaacct cgaagcctgc cacaccatcg cccgcgaatt
     2941 gaggctacgc aacctcggcg gcatcatcat catcgacttc atcgatatgg cgcaggaaag
     3001 ccaccgcgaa gccgtgttgc aggagcttgc caaagccctc gccttcgacc gtacccgcgt
     3061 taccctgcac ggttttacca gcctggggct ggtcgagctg acgcgcaaac gctcgcgcga
     3121 aaacttaaac caagtcctct gcgaaccctg cccttcctgc caaggcagag gccgtctgaa
     3181 aacgccgcaa accgtgtgct acgaaatcca gcgcgaaatc gtccgcgaag cgcgccgtta
     3241 cgatgccgaa agtttccgca tcctcgccgc ccccaacgtc atcgatttgt ttttggacga
     3301 agaatcgcaa tccttggcaa tgctgataga tttcatcggc aaaccgattt ctctggcggt
     3361 cgaaaccgct tacacgcagg aacaatacga tattgtgctg atgtaaaaaa tgccgtctga
     3421 agccttcaga cggcatctgt ctatttcagg gtttccttgt ccaacaaggc gcgtatcagc
     3481 agaccgtgtc cgaacctgcg gctgtcggtc agttccaaat atccgccgta ttttttggct
     3541 agcgtgtcgg caatcgacag ccccagcccc gtcccctgct gctccgttcc caaaatacgg
     3601 taaaacggat cgaggacgcg gacgcgttcg gattccggaa tgccgttccc gttatcttcc
     3661 acccacacgg caagatattt cccttcgtct gtgaaaccca aatctatcct gccttcggac
     3721 ggggtataac gtattgcgtt atcaacaaac gttttaacca aggtatagat ttcagtttca
     3781 tcggcaaata tttcgacatc acctccgacc gccacaccaa tgtcctgacg tttttccaag
     3841 gcaagcggca tcagttcctg caacacttgg cggaaacggc tttgcagacc gaatgtcgtt
     3901 ttcgccaaag gggtttcgtc cgactgcgaa cgcgccagtg ccaaaagctg ctcgagcagg
     3961 tgtttgttgc gccgtatgct ttgctgcaaa acggcagact gccgcgccgc atcgggtggc
     4021 agagacatat tgttgagccg ttccgcctga agggaaaggg ctgtcatcgg cgtacgcaat
     4081 tcgtgtgccg cgtcggcgac aaaccgctga cggtggcgga tgtcttcatc cgcacgtttc
     4141 aaaagcaagt tgatggcggt tacgaaccct ctgatttcac tgggaatatt gtccacactc
     4201 aaggcagaca ggtcattgat tcggcgttgt tcgagacttt gcgacaattt gcggacgggg
     4261 cgcatggctt tgtgcgtaat ccacacggtc agcagtatca tcagcggcag tgccgccagc
     4321 aggggcaaca cgctttgccg tgccgcatcc gccgccaaat cttcccggta ttcgctttcc
     4381 tgcatcacgg caatccgccc ccgctccgtc gtgcggatat agacgcggta ataatcgtcg
     4441 tccccgtccg cctgaagcgt gtgcagaccg tccgccagat gcgcaggcag gctgacaaca
     4501 gggtcttcct gctgcggcat ctgcacgaaa atgcgcgtat cgccgtcgcc ctcgggcaaa
     4561 gtttcgggtt tggaatcggg tgcgacgtac aatgccgcct gacggagcag gtcgtcctgc
     4621 aacgcttccg tttcgtggaa ggtttcgtag taggaaaaca tacctgcaag cattgccagc
     4681 ggaacaaaca tccaaaccgt cccgccccag caaacccaaa tccagcagca tcaagtcata
     4741 aggctgggca gcggcagccg cgcgccgttt ttgacccaat ccaccgcata gccgccgtct
     4801 ttcaaacttg ccgacaccgc ctccgcaatc atcgcatcgt cttccaccag caaaacacgc
     4861 atcaactttc ccttcaaaat aaaccgtgcc tattctaaca ccccaaaatt agccgcaatt
     4921 tagcggcctt tacgcttgcc ggtatttttc aaaactgcgg cacaaaaaac cgcgccggca
     4981 actgccttca gacggcattg gggcgcgatt gcaacacacg ggcaggcaga gcctgcgaca
     5041 gaccacagga acgattcagg cttcagacgg cttcgccgtt tacggcagag gcacgattcc
     5101 tgccgctatc gaactggcca atatcgccag cgacaaaccc cacgcccaga aaaacgaata
     5161 acggatgtgt ttgcccatcg acaatttcgc caaacccaag cccatccaca aagccggcga
     5221 aagcggcgta acaaaagtgc cgacgatact gccgatcaac atcgcataac ctgctgcttc
     5281 gggcgccacg cccgcctgcg aggtaatctg ctccacaatc ggaaacagtc cgaaataata
     5341 agcgtccgta ctcaaaacca actcaagcgg aatgcccaac acaccgatgg caatatgcag
     5401 ataaggcagc agcgcgtccg gcaggatatg cacaatgtct ttggaaatcg cgtccaacat
     5461 ccccgcaccc ttcaaaatcc ccaaaaacgt acctgccgcc aaaataatgg acgccatcat
     5521 caccgcgccg ccggcgtggg cataaatccg ctccatctgt tcctgcgggc tgcggtaatt
     5581 caaaagcaac gccgccgttg cagccagcat aaatacataa cccggcggga agatgcccga
     5641 aaaaagcagg ctcatcgccg ccaaaaacag caggacattc caccaaaaca gtttcggacg
     5701 cgccaatttt tgttcttctt ccgacaaagg caccggcttt atcaaatccg ccacggcggg
     5761 caacgcgccc aactcccgga caatccgcct tttttcacgc acacccaaaa gcagggacag
     5821 cgcaaggata aacaccacac cgataatttg caccgtcaac aaaggtttat acaattcgcc
     5881 cacatctgcg cccaacacgc ttgcaacccg cccagtcggc ccgccccacg gcagaaggtt
     5941 aatcaatccc gcactggaag ttagcagcaa aaacagcagg taaggattca tatgcagacg
     6001 cttgtaaagc ggcaaaaggg cggggacgac caataaaaac gtcgtcgcac ccgccccgtc
     6061 caactgcgcc accaccgaca ccaagaccgt ccccacactc actgccacga tattaccccg
     6121 agtcagctta atcaaaccgc ctatcatcgg acggaacagc cccacatcgt tcatgattcc
     6181 aaaaaacaaa atggaaaaca taaacataat cacaatctgc atcaccgatt tggtgccgcc
     6241 cgaataaaat tcttttaatt gggatacatc aaaccccgcc agcaacgccc caaacagcgg
     6301 caccaagatt aatgcgatga tgggcgacac tttttccgtc agcagcaacc atacgatgac
     6361 cccgataatc agcagtccga taaacgtcag catcatttct cctttatttt attttaaaca
     6421 gaaaaccgac cgtgcaggca aaaccgccca cagacgcggg ataagccttg cattctactt
     6481 ttttattttg aaacaagtca atcggtcatt tcctcccatt tacgcctgcc gccattcctg
     6541 tatccgtccg tcatttcacg gcggcaaccg atacggaaca accggcaaac cggtatcggg
     6601 acggcgcggg ggcattcatc ccggtgcgcc gattcaaacg aaaccgcccc tatcattgcg
     6661 gagcgcgggg cgtgccgtac ac
//
LOCUS       DAWHRA010000112         6544 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWHRA010000112 DAWHRA010000000
VERSION     DAWHRA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.112"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1308..2387)
                     /gene="apbC"
                     /locus_tag="WIB30_001657"
     CDS             complement(1308..2387)
                     /gene="apbC"
                     /locus_tag="WIB30_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ3797693.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPETGDTHIHLSIDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            2603..2678
                     /locus_tag="WIB30_001658"
     tRNA            2603..2678
                     /locus_tag="WIB30_001658"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2636..2638,aa:Lys,seq:ttt)
     gene            2757..3266
                     /locus_tag="WIB30_001659"
     CDS             2757..3266
                     /locus_tag="WIB30_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ3797694.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            3609..4049
                     /gene="nadR"
                     /locus_tag="WIB30_001660"
     CDS             3609..4049
                     /gene="nadR"
                     /locus_tag="WIB30_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ3797695.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            4164..4664
                     /gene="hpaC"
                     /locus_tag="WIB30_001661"
     CDS             4164..4664
                     /gene="hpaC"
                     /locus_tag="WIB30_001661"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ3797696.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRKARIIPILSENGSLCINTLADEHQDVAEHFAGLTNLSPEERFAY
                     HIWHYGKTGQPEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            4680..5375
                     /locus_tag="WIB30_001662"
     CDS             4680..5375
                     /locus_tag="WIB30_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ3797697.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(5621..6052)
                     /locus_tag="WIB30_001663"
     CDS             complement(5621..6052)
                     /locus_tag="WIB30_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ3797698.1"
                     /translation="MGSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGLNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLNERPSTQEWIGLGLVTAGVLVLALKR"
     gene            6227..>6544
                     /locus_tag="WIB30_001664"
     CDS             6227..>6544
                     /locus_tag="WIB30_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ3797699.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLLQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVK"
BASE COUNT         1388 a         1640 c         1939 g         1577 t
ORIGIN      
        1 ggcataaagc gcggtatggc ggaaaacctg cctttttaaa gttttattca tcgtatttcc
       61 ttttcggtca aaccccccgc ccttcgggca tccgtccttc ggggcggcag gatcaggctt
      121 tgtccgggag gggtgcaagc ccttccgaat cagggcggcg ccttatgtgc cgccctgcgt
      181 gttggaacat attttatccc ttaaatcttg cttcgcggga taccgtcggc ggcaggcacg
      241 ccgcaaacgg cgggattcgg caaaccgccc gattccgcgc ccgccgaatt gctggttgga
      301 aaaccccttt ggcccggctg ccaaaggggg atgttcggaa aagtaacacg cccctttggc
      361 cggatgcaat gtatagggtt accgcattat tctacccttg cgcgctgatc atggtaaagc
      421 ggttttcggc aatccgcaac cgcctccttg gcccgccatt gccgcgaaag cgggaatccg
      481 gaacgtaaaa tctcaaaaaa accgttttat ccggcaagtt tgcccgccgg caggcccgcc
      541 attgccgcct gcgcggcaat ggcgggccgg ggaggcggca tttttcaagt tgcggcaatc
      601 tttcccaaca actcaaccgc cccaatacaa gcctttcggc ttgttgccct ctctctagct
      661 ctctcccaca gggagagagg acgggtcggc tgttggggtt gaggtttgct gcaaggaaaa
      721 caggttgtgc aggctgcttt ttatttttca gacgacctga aacaacagct gcaaatctaa
      781 gttgcctttc aaatcgcctg caaccgaaaa atctgccgaa acccactccc tcccccgtgg
      841 gggagggccg gggaggcggc attttccaag ttgcggcaac ctttcccaac aactcaaccg
      901 ccccaataca agcctttcgg cttgttgccc tctctccagc cctctcccac ggggagagag
      961 gacggggagg ctgttggggt taagggtttt gtaaactaat caggttgtgc aggctgcttt
     1021 ttgcttttca gacgacctgc atcaacggtt gtaaatccaa tctgtttttc aaaccgcctg
     1081 caaccgcaaa acctgccgca atccgctccc tcccctgtgg gggagggccg gggagagggc
     1141 attctccaag ttgcggcaat ctttcccaac agcttaaccg ccccaataca agccttgcgg
     1201 cttgttgccc tctctccagc cctctcccac ggggagagag gactatgaag cccgccggca
     1261 ggcgcggggc ttgggacggc attgctgttg cggtttcgga cgcggcttta ttcgacgacg
     1321 attttgggga agcggctgct gaagtctttg cctttgtcgg caatgcccag ggcgatttgg
     1381 aatgcggcat cggtgtagat tttggcgatg gcggggtgtt cgtcgaacag ttgcgccggt
     1441 gtgccgccgt ccatggcttc gcgcacgggc aggcttaggg gaagctgtcc gagcaggggg
     1501 acgttgaggc gtgcggctaa atctttgccg ccgtccgtgc cgaacagggc ttcgctgtgt
     1561 ccgcagttgg tgcagatgtg gacggacata ttttccaata cgcctaaaat gggaatgttg
     1621 accttgcgga acatatccac ggctttgcgc gcgtctatca gggcgatgtc ctgcggcgtg
     1681 gttacgatga cggaaccggt tacggggatg cgctgggaca gcgtgagctg gatgtcgccc
     1741 gtacccgggg gcaggtcgat gaaaagatag tccacttcgt cccactcgct ttggaacatc
     1801 agctgctgca aggcttggct gaccatcggc ccgcgccaga cgacggcttg gtcggtatcg
     1861 acgagaaagc cgatagacat gacctgtatg ccgtctgaag attcgacggg aatgagtttt
     1921 tggtttttct gatcgggttt gcggtcgtcc acacccaaca tggtcggttg gctcgggccg
     1981 taaaggtcgg catcgagcac gccgacgcgc gcgcccatcc gcgccattgc ggcggcaagg
     2041 ttggctgtcg tcgtcgattt gcccacgccg ccttttcccg atgcgacggc gatgatgttt
     2101 ttcacgcctt tgatggtggt aacgccgggc tggactttgt gtgtgccgat ttcggtgtct
     2161 atggacagat ggatgtgtgt gtcgcctgtt tcgggcatca ggatttcttg tatgcggtcg
     2221 gctgtttctg aggcaatgtg cgcgacgggg aagccgaaat gcagggcgat atggatgccg
     2281 tctgaacgct gttcgaccga acggacggcc ttttcgccgc cgagcgtgcg tgccgtattc
     2341 ggaacggcga cggtgtcgag gagggtgcgg atgttttgta tgttcataat gtgtgtttct
     2401 gtgtgcagtt tgggaaggga aacgagcgga aagtgtacat tgatttcggg cggtttgctt
     2461 ttccttgtgc gaacttttac acggtatgcg gcggattgtg aaaatccgtt gtcaaaatat
     2521 cccgtgcaaa cagtggtctg caattaagcc tgtaaaattt gcttgacgag gcaggatggt
     2581 gctgtataat gcacggctta tcgggtcgtt agctcagtcg gtagagcagc ggacttttaa
     2641 tccgttggtc gagcgttcga atcgctcacg acccaccaga taaccggaag ccaagttttg
     2701 agacttggct ttttgtttgc ctgccggttg cgtgggcgta ccgtttgaga gagcgtatga
     2761 aaaagaaact gctttcgggc atcaaatttg ccgttcagac ggcattggtg tttttattgg
     2821 tgtcgctgtt tttggattgg atacgcaagc ccgaagagcc ggccggtgcg gcagggcggc
     2881 ctttgaccct gctgtcgggg cagcggctga ctttgggaca gtttagccgg gataggacgg
     2941 tgctggtgta tttttggggg agctggtgcg gcgtgtgccg ttatcagtcg ccgataatcg
     3001 atgatttggc ggcggacggc gttccggtcg tcggcgtggc ggtacgttcc ggcagcgcgt
     3061 ccgaagtggc ggcgtatatg gcaaagcggg gcttgggctt tccgacggtc aacgatgagg
     3121 acgggggttt ggcaaggtct tggcggattg tcgcaacgcc tgccgtcgtt ttggtcaaaa
     3181 atgggaaaat ggttcactat acgacgggaa tcagcagtta ttggggcttg cgcgcacgaa
     3241 tttttcaggc ggatgttttc ggttaacttt tgtttttgtt aaactttacg ctatgtggag
     3301 aatcaaacgg catatacctt gctcgtctga tgcggcggct tttgtttttt ctggaagaaa
     3361 gttctttcga cagtaaatgt aatactttgt tgagattgta gggaaaatag actaagatat
     3421 aactattaaa atatttttag ataggattat cggaattaaa gtcttttata cccagtcgtc
     3481 cgatgcggtt tatagcgtat tgttgctata tgttcgtttt gttatataac ggttgcatca
     3541 aaaattacgc ccacaggctt tcccgacggt ttgaaagttt gattttcgat aacttggaga
     3601 cttgaacaat gcctacccaa tcaaaacatg cgtctatcaa tatcagtctg atacaggcaa
     3661 gggaagccct gatgacccaa ttcaggccta ttctgaatca ggcgaatatt accgatcagc
     3721 aatggcggat tatccgtctt ttggcggaaa acggcacgct ggactttcaa gatttggcga
     3781 atcaggcgtg tattctgcgc cccagcctga ccggtatcct gacccgcctt gaaaaagcgg
     3841 gtttggttgt ccgcctgaaa ccttccaacg accaacgacg tgtttttctg aagctgactg
     3901 ccgagggcga gaagctgtat gaggaaatcg gcgaagaagt ggacgagcgt tacgacgcta
     3961 tcgaggaagt gctgggccgc gagaaaatgc tgctgcttaa agacctgttg gcagaacttg
     4021 ccaaaatcga ggatgcgttg aactcgtaat acgccgtaac gcgcggaaat gtccgaccga
     4081 cggctttttg aatcagaact gctgcacatg gggggtgcct tgtgtgcagc attcttatat
     4141 aggggacagt ttaaagggga aaaatggcgg atttgcagaa aacttttcaa acttcgttcc
     4201 gtgatgcgat ggcatcctgt gcggcaggcg ttcatgtcat cacgacagac ggtgcggcag
     4261 ggcgttacgg cattacaatg acggcggtcg cgccggtaac ggacgagccg ccgaccgtga
     4321 tgctgtgcat caaccgtaaa gcccgtatca tcccgatact gtcggaaaac ggcagcctgt
     4381 gcatcaatac gttggcagac gaacatcagg atgtggccga acattttgcc ggattgacca
     4441 atctttcacc cgaagaacgt tttgcctatc atatttggca ttacggtaaa accggacaac
     4501 ctgaaataga aggcgcgttg gcgcacctgc acgggcatat tgtcggcaaa catgaaatcg
     4561 gcacgcattt tgtgttttac gtcaggctcg acgaaatcaa aaactgcggg tgcaaacgcc
     4621 ccgcgctgct gtatttcaga cggcagttta gatttttaga ctgatattcg gacagatata
     4681 tgaaagcgat gatactggcg gcaggacgcg gcgagcgtat gcgccctttg accgatacca
     4741 ctccgaagcc gctgctcgat gtggcgggta agcctctaat cggttggcac ctatgccgtc
     4801 tgaagcaggc ggggtttacc gaaatcgtca tcaaccacgc ttggctgggt cggcagatag
     4861 aagatgcttt gggcgacggc tcggcttatg gcgtgaacat cgcctattcg cccgaacccg
     4921 caggcggttt ggaaacggca ggcggcatcg cgcaggcatt gccgctgttg ggtgggcagc
     4981 cgtttttggt ggtcaacggc gacgtgctga ccgacatcga ttttaccgcc gcgtttcagg
     5041 cggcatcgtc cctgccggaa catatttccg cccatctgtg gctggtggaa aatccgccgc
     5101 acaaccccga cggcgatttt tccctgctgc ccgacggcag cgtgcggccg gaagtaaatg
     5161 gcggcaacgg attgacattc agcggcgtgg gtatttaccg tcctgaaatg tttgacggaa
     5221 tcgaagcggg cagtgtggcg aaactcgcgc ccgtattgcg tggcgaaatg cggcaaaacc
     5281 gcgtgagcgg tcagaagcat acgggcttgt ggctggatgt cggcacggta tgccgtctga
     5341 aagaggctca agcccttgca ggggcttgga agtgaaataa aacggtggct tgccgagccg
     5401 gctgccgaac ggttccgacc ggatgtccag catattttcg tggaggcgtg aaaatagggc
     5461 gttcggggct ttccgtccaa caaatgccat ctgaagccga cccctgtcgg atgcggcggt
     5521 ttgccgtcgg cgcggtgtcg gattttattg ggtaactttg ttcagaccgc cgcccgtcag
     5581 attgtcaaaa cccggtttca ggcggtatgg cggattcggt ttaacgtttc aacgccagca
     5641 ccaacacgcc cgccgttacc agccccaagc ctatccattc ctgcgtgctg gggcgttcgt
     5701 tgaggaagac tactgccatc agggcgacca gaaccaggct gaatttatcg acgggggcga
     5761 cttgcgaggc gttacccagt tgcagggctt tgaagtaggc gagccaagat gcgccggtag
     5821 cgaggccgga taggatgagg aatgtccagt tgaggccggt aaagccgttc acgccctgcc
     5881 atttgccggt gtaggttaaa aacaatacca aagcggcgag gatgaccaag gtgcggataa
     5941 aggtggcgaa atccgaatct atgccctgta aacccatttt ggcgaaaatg gcggtcaatg
     6001 aggcgaagcc tgccgatgcc aatgcccaaa acagccatgc gttgctgccc atgttttctc
     6061 cttggattgt gaacaatatg aacggtattt ttgttgcagc gtcaaaaatt tcactgcggg
     6121 tttggtgcgg ataacgttat aatatgcctg atattatttt caatccacct gtttgtcgcc
     6181 tgatgctttc agacagtatg tccctcctca tttctaaagg aaaatcatga gcttcaaaac
     6241 cgatgccgaa atcgcccagt cttccacgat gcgcccgatt ggcgaaattg ccgccaagct
     6301 gggtctgaac gccgacaaca ttgagcctta cggtcattac aaggcgaaaa tcaatcctgc
     6361 cgaagcgttc aaactgctgc aaaaacaggg caggctgatt ttggttaccg ccatcaaccc
     6421 gactccggcg ggcgaaggca aaaccaccgt aaccatcggt ttggcggacg cgttgcgcca
     6481 catcggcaaa gatgccgtga ttgccctgcg cgaaccttct ctggggccgg tgttcggcgt
     6541 gaaa
//
LOCUS       DAWHRA010000113         6512 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWHRA010000113 DAWHRA010000000
VERSION     DAWHRA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.113"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            150..1691
                     /locus_tag="WIB30_001665"
     CDS             150..1691
                     /locus_tag="WIB30_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ3797700.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGGYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETRQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDGGEGGK
                     QTETL"
     gene            1720..2319
                     /locus_tag="WIB30_001666"
     CDS             1720..2319
                     /locus_tag="WIB30_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ3797701.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2316..2921
                     /gene="nadD"
                     /locus_tag="WIB30_001667"
     CDS             2316..2921
                     /gene="nadD"
                     /locus_tag="WIB30_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ3797702.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            2980..3366
                     /gene="rsfS"
                     /locus_tag="WIB30_001668"
     CDS             2980..3366
                     /gene="rsfS"
                     /locus_tag="WIB30_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ3797703.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3420..3890
                     /gene="rlmH"
                     /locus_tag="WIB30_001669"
     CDS             3420..3890
                     /gene="rlmH"
                     /locus_tag="WIB30_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ3797704.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAASILHNHPYHRE"
     gene            3963..4229
                     /locus_tag="WIB30_001670"
     CDS             3963..4229
                     /locus_tag="WIB30_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ3797705.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4386..5069
                     /locus_tag="WIB30_001671"
     CDS             4386..5069
                     /locus_tag="WIB30_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ3797706.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5193..5705
                     /gene="coaD"
                     /locus_tag="WIB30_001672"
     CDS             5193..5705
                     /gene="coaD"
                     /locus_tag="WIB30_001672"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ3797707.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5737..6390
                     /locus_tag="WIB30_001673"
     CDS             5737..6390
                     /locus_tag="WIB30_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ3797708.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1415 a         1709 c         1880 g         1508 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ttcagacggc gggaggaagg
       61 cggatacggt atgtagtcgg attgaaatat acggtatgtg ggggcttttg ggtacaatcg
      121 caacatcagt gtttcaagtg gggaaggcga tggatggacg gcgttgggcg gtatggggtg
      181 cttttgccct gctgccttcg gcttttttgg cggtaatggt cgttgcgcct ttgtgggcgg
      241 tggcggcgta tgacggtttg gcgtggcgcg cggtgctgtc ggatgcctat atgctcaaac
      301 gtttggcgtg gacggtattt caggcagcgg caacctgtgt gctggtgctg cctttgggcg
      361 tgcctgtcgc gtgggtgctg gcgcggctgg cgtttccggg gcgggctttg gtgctgcgcc
      421 tgctgatgct gccttttgtg atgcccacgt tggtggcggg cgtgggtgtg ctggctctgt
      481 tcggggcgga cggcctgttg tggcgcggac ggcaggatac gccgtatctg ttgttgtacg
      541 gcaatgtgtt tttcaacctt cctgtgttgg tcagggcggc gtatcagggg tttgtgcaag
      601 tgcctgcggc acggcttcag acggcacgga cgttgggcgc gggggcgtgg cggcggtttt
      661 gggacattga aatgcccgtt ttgcgcccgt ggcttgccgg cggcgtgtgc cttgtcttcc
      721 tgtattgttt ttcggggttc gggctggcat tgctgctggg cggcagccgt tatgccacgg
      781 tcgaagtgga aatttaccag ttggtcatgt tcgaactcga tatggcgggg gcttcggcac
      841 tggtgtggct ggtgttgggg gtaacggcgg cggcagggtt gctgtatgcg tggttcggca
      901 ggcgcgcggt ttcggataag gcggtttcac ccgtgatgcc gtcgccgccg cagtcggtcg
      961 ggggatatgt gctgctggcg tttgcggcgg cggtgttgtc tgtgtgctgc ctgtttcctt
     1021 tgttggcaat tgttgtgaaa gcgtggtcgg ccggcgaatc gtggcgtgtg ttaatggaaa
     1081 gtgaaacgag gcaggcagtg tggaatactt tgcgcttctc ggcggcggcg gtgtttgcgg
     1141 cggcggtttt gggtgtggtg tatgcggcgg cggcgcggcg gtcagcgtgg atgcgcgggc
     1201 tgatgttttt gccgtttatg gtgtcgccgg tttgtgtttc ggcgggcgtg ctgctgcttt
     1261 atccgcagtg gacggcttcg ttgccgctgc tgctggcgat gtatgcgctg ctggcgtatc
     1321 cgtttgtggc aaaagatgtt ttatcagcct gggatgcact gccgccggat tacggcaggg
     1381 cggcggcggg tttgggtgca aacggctttc agacggcatg ccgcatcacg ttccccctct
     1441 tgaaaccggc gttgcggcgc ggtctgactt tggcggcggc gacgtgtgtg ggcgaatttg
     1501 cggcgacatt gttcctgtcg cgtccggaat ggcagacgtt gacgactttg atttatgcct
     1561 acttggggcg tgcgggtgag gataattatg cgcgggcaat ggtgttgaca ttgctgttgt
     1621 cggcgttcgc gctgggtatt ttcctgctgt tggacggcgg cgaaggcgga aaacagacgg
     1681 aaacgttata atgtgaaccc tttttcagag gacggcaaaa tgagcgagtt ggataacatc
     1741 cttgcccata accggcagtt tgtcgagtcg ggcgaatatg aaaaatactt taccgacaaa
     1801 taccccgaac gcgggctggc agttttgtcc tgtatggatg cgcggattat cgggctgctg
     1861 cccgacgcgt tgggtttgaa aaacggcgat gccaagctga ttaaaaatgc cggcgcgctg
     1921 gttacgcacc cgtggggttc ggtgatgcgg agtcttttgg ttgccgtgtt tgaattgaag
     1981 gtcagagaga ttatggtcat cgcccatcac gattgcggta tgcaggggct gaatgccgaa
     2041 gaattcctcg ggcgcgtccg ggaaagccag attcccgaag accgtatcga aaccctgcgt
     2101 tatgccggta tcgacctcga cggctggctg accggtttcg acaacgtcga agacagcgtg
     2161 cgccacacgg tggaccttat ccgtaaccat ccgctgatgc cgcgccatat cgccgttcac
     2221 ggactggtca tccatcccgt taccggcaaa ctgacgctgg ttgtggacgg cagtgtttca
     2281 gacggcatgg acttatcgga aggaatggaa acatcatgaa gaaaatcgga ctgttcggcg
     2341 gcactttcga cccgatacac aacgggcatc ttcatatcgc ccgtgccttt gccgatgaaa
     2401 tcggcttgga cgcggttgtt ttcctgccgg caggcggccc gtatcacaaa gacgcagcct
     2461 ccgcttccgc cgccgaccgc cttgccatgg tcgaactggc gacggcagaa gacgcgcgtt
     2521 ttgccgtcag cgattgcgac atcgtccgag aaggtgcaac ctatactttt gataccgtcc
     2581 aaatcttccg ccagcagttc ccatccgcgc aactttggtg gctgatgggc agcgacagcc
     2641 tgatgaagct gcacacatgg aaaaaatggc agatgctcgt gcgcgaaacc aatatcgccg
     2701 tcgccatgag gcagggcgac agcctgcacc aaaccccgcg cgaactgcac gcgtggctgg
     2761 gcaagtccct tcaggacggc agcgtccgca tcttgtccgc cccgatgcat aatgtgtcgt
     2821 caacggaaat ccgccgcaac cttgctggcc aaggcgtttc agacggcatc ccgcctgccg
     2881 ccgcacgcta catccgcgaa cacggtttgt atgaaaaata aagtcaaatc agtaagaaac
     2941 ggctataatg ccgtctgaaa acatcccgtt aggaacataa tgaacgaaca agaactgcaa
     3001 gacctgcaaa aaatggtcgg ggtcgccgtc aacgccctcg aagacatcaa agccaaagac
     3061 atttccgttc tcgaaacgca agacaaaact tcgctgtttg ccagaatgat tatcgccagc
     3121 ggcgacagta cgcgccaagt caaagcactg gccaacaacg ttgccgtcga tttgaaagaa
     3181 gccggttttg aaatcctcag taccgaagga gacagcggcg aatggacgct cgttgatgca
     3241 ggagacctcg tcgtccacgt tatgctcccc gccgtgcgcg acttctatga tatcgacacc
     3301 ctctggggcg gcgagaaacc gagtttccac gccggaatgc agaagccgtg gcacgcggca
     3361 gactgattcc cgatgccgtc tgaatgttca gacggcattt tcctgtaagg gagaaagcat
     3421 tgaacatcac cgttttggca gtcggcacca aaatgccgcg ctgggttgat gaggccgtcg
     3481 ccgaatacgc caaacgcttc ggacgcgacg tcgcctacgc actcaaagaa atcaaacccg
     3541 aaaaacgcgg cgcgggcgtg aatgccgccc aaggtatggc ggcggaagaa aaacgcatcc
     3601 ttgaagccat tccgcaaggc gcgttcctcg tcgttcttga cgaacgcggc aaagcaccga
     3661 cctccgtcga gctggcggaa cacctcaaaa gttggcagca aaacggcgaa cacgtctgct
     3721 tcgtcatcgg cggcgcggac ggcatgaccg accgcctcaa acaacaagcc cgcatgatga
     3781 tgcgcctgtc cagcctcacc ctgccgcacg gcatggtgcg cgtctttctg accgagcagc
     3841 tctaccgcgc cgcctccatc ctgcacaacc atccctatca tcgggaataa gaaggctttt
     3901 gtccgtatcc ctaatcggtt aaaattaccc cgttatttct gaacacagca aaggaatccg
     3961 ctatggcacg catggtattc tgcgtcaagc tcaacaaaga agccgaaggc atgaaatttc
     4021 cgccgcttcc caacgaattg ggcaagcgca tttttgaaaa cgtatcgcaa gaagcgtggg
     4081 cggcgtggac gcgccaccaa acgatgctga ttaacgaaaa ccgtttaagc ctcgccgatc
     4141 cgcgcgcgcg cgaatacctg gctcagcaga tggagcagta tttcttcggc gacggcgcgg
     4201 atgccgtgca gggatacgtt ccgcaataac ggttttccgt tttgaacaca ggctgtccga
     4261 aactgcttca gacggccttt aaaatacgcc ggacaacttt atcttcacgc aaccggacaa
     4321 attttgacat tgggcaatgt taaaatcccc cattattccc aaccgcgctt tcaggagcag
     4381 atgatatgca acacgacgtt tacgactaca ccgcgcatac ggtttctaaa aacaccgtcc
     4441 tgcagaaaac ctaccgcctg ctcggatttt cattcattcc ggctgccgca ggcgcggcac
     4501 ttgccgccaa tgccggtttc aatttttacg ccgccttcgg ttcgcgctgg atagggtttg
     4561 ccgtcgtgtt ggcgtttttc tacggtatga tccacttcat cgagaaaaac cgttacagca
     4621 ataccggcgt taccctgctg atggtattca cattcggtat gggcgtattg atcggccccg
     4681 tgctgcaata cgcgctccat attgccgacg gtgcgaaaat cgtcggcatt gccgccgcga
     4741 tgaccgccgc cgtcttttta acgatgtccg ccttggcgcg ccgaacccgg ctcgatatga
     4801 acgcgctcgg acgcttcctg accgtaggtg cggtcattct gatggtcgcc gtggttgcca
     4861 atctgttttt gggtattccc gcactcgccc tgaccatttc cgccggtttt gtcttgttca
     4921 gttccttaat gattatgtgg caggtacgca ccgtcatcga cggcggcgaa gacagccaca
     4981 tcagcgcggc actgacactg tttatctcgc tttacaacat cttcagcagc ctgctgaaca
     5041 tcctgttgtc tttaaacggc gaggattgat gtcggtaaaa cgccgtccga ctatgccgtc
     5101 tgaaaatgct tcagacggca ttttactttg ggcatacaat acggatgcac acgcaccaac
     5161 acacagccgg ctatgaagcc gggaggaatc aaatgttacc gaatacaccg cgccgcgccg
     5221 tttatgccgg cagtttcgat ccgcccacat tggggcatct gtggatgata cggcaggcgc
     5281 aatctatgtt tgacgaactc atcgtcgcca tcggcattaa ccccgacaaa cgcagcacct
     5341 ataccgtcgc tgaaaggcag gatatgttgt gcgatattac taaaatgttt cccaacgtca
     5401 gaaccgatgt atttgaaaac cgatttctgg tgcattatgc ccgtgaggta gatgcaggat
     5461 tcatcgtgcg cggcatccgt tctgcttcgg attacgaata cgaacgttcc atgcgccaca
     5521 tcaacagcga cctcgccccc gaaatatcca ccgtattcct catgccgccg cgcgaaatcg
     5581 ccgaagtgtc gtccactatg gtcaaaggac tggtcgggcc ggaaggctgg acggaaacga
     5641 tccaccgcta cgtgccgcaa gctgtgtacg aaaaaatcct tgccgaacat caacacgaaa
     5701 attgaaccct tctatttttg caaggcatat atggatatgt tggaaatttt gttccgccat
     5761 caagacttcg tcgccatcaa caaaccgggc ggcatatccg tccaccaaga cagcggcgag
     5821 accggactgg cccgaacact cgccatacag ttgggcgtag agcgtgtgtg gctgctgcac
     5881 cgattggaca aacagaccag cggcatttta ctgtttgccc tcaacagaga aagcgcgtcc
     5941 gcccttagcg gacagtttgc cggcaaaagc atcaaaaaaa cctatttggc actatccgac
     6001 cgcaaaccgt ccaaaaagca gggttggatt aaaggcggaa tggaaaaatc cagacgcgga
     6061 atgtggaagt tgacgcgcaa tacagaaaat atcgccgtta cccgattcca cagcatcagt
     6121 atcgccgaaa aactgcggct gttcatcctg gaaccgcata cgggcaaaac gcaccaattg
     6181 agagtggcga tgaaaagttt gggcagtccg attctgggcg acagcttata cggcggaaca
     6241 gaatccgaaa ccatgtttct gtatgcatgg aaaatacaat ttgcctacca aaaccgacaa
     6301 atcgaaattg ttgcaccctt aaaaaacgaa tggcagactg aaaatatctc tcatgcgctg
     6361 gaagaatttt atatggcaaa agcaggttaa taaatataaa ttaatttata aacaatatga
     6421 taaatatttt tataaaaatt tttcaagaaa acggttgact gcaaatctct tcaagcgtat
     6481 agttcggttc ttcgctgctg cagaagcggc gg
//
LOCUS       DAWHRA010000114         6435 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWHRA010000114 DAWHRA010000000
VERSION     DAWHRA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.114"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            89..298
                     /locus_tag="WIB30_001674"
     CDS             89..298
                     /locus_tag="WIB30_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ3797709.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            387..1601
                     /locus_tag="WIB30_001675"
     CDS             387..1601
                     /locus_tag="WIB30_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ3797710.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVYQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="WIB30_001676"
     CDS             complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="WIB30_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ3797711.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LEKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4528..5538
                     /gene="trpS"
                     /locus_tag="WIB30_001677"
     CDS             4528..5538
                     /gene="trpS"
                     /locus_tag="WIB30_001677"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ3797712.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5544..5885
                     /locus_tag="WIB30_001678"
     CDS             5544..5885
                     /locus_tag="WIB30_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797713.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6205..>6435
                     /locus_tag="WIB30_001679"
     CDS             6205..>6435
                     /locus_tag="WIB30_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797714.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAK"
BASE COUNT         1544 a         1682 c         1629 g         1580 t
ORIGIN      
        1 gatttgaatt tttcagaaaa tgaagcggac gtttgggcgg aggcaacttg tttgataaga
       61 tagcaatatt tcaaaacgga gaaagatcat gccttatgtc aatattaaag taaccggcgg
      121 caaggaagca ccgactgccg ctcaaaaagc ggaactgatc ggcggcgtaa ccgaattgct
      181 ggcacgcgtg ctgggcaaaa atcccgaaac aacggttgtc gtgattgatg aagtggatac
      241 cgataactgg ggaataggcg gcaaaagcgt cagcgaacgg cgcaaagagg gcaggtaaaa
      301 agcctgaaaa tctcggtttg atgctttaaa ttccgcgtga aaaagagtac attcccaccc
      361 attgcccaaa atttacggaa cacatcatgg ataaatttcc caagtctgca aagctcgatc
      421 acgtctgtta cgacatacgc gggccggttc acaaaaaagc acttcagttg gaagaggagg
      481 gcaataaaat ccttaaactc aatatcggca accctgcgcc gttcggcttt gaagcccctg
      541 atgaaatctt ggtcgatgtc atccgcaacc tgccgacttc gcaaggctat tgcgattcta
      601 aagggctgta ttccgcccgc aaagccattg ttcactacta tcagaccaaa ggtttgcgcg
      661 atattacggt tgatgatgtc tatatcggca acggcgtgtc cgagctgatt acgatgtcta
      721 tgcaggcgtt gctcaacgac ggcgacgaaa tcctgattcc cgcgcccgac taccctttgt
      781 ggacggcggc ggcaacgctt gcgggcggta cggtacgcca ttatctgtgc gacgaagaaa
      841 acggctggtt ccccaacctt gccgatatgg aagccaaaat cacgcccaaa accaaagcca
      901 tcgtcgtcat caatcccaat aatccgacag gcgcggtgta cagcagggaa atcctgttgg
      961 aaatcgccga actggcgcgc aagcacggtt tgattatttt cgccgacgag atttacgaca
     1021 aaatccttta tgacggcgcg gttcaccacc atattgccgc gcttgccccc gaccttttga
     1081 cggtaacgtt caacggtttg tccaaatcct accgcgtcgc cggcttccgc caaggctgga
     1141 tggtgctcaa cgggccgaaa catcacgcaa aaggttacat cgagggtttg gatatgctct
     1201 cgtccatgcg cctgtgtgcc aatacgccga tgcagcacgc gattcagacg gcattgggcg
     1261 gttatcagag cattaacgaa ttcattttgc ccggcgggcg gcttttggaa cagcgcaaca
     1321 gggcgtggga actggtcaac cagattcctg gcgtgtcctg cgtcaaaccg atgggtgcga
     1381 tgtatatgtt cccgaaaatc gataccgaaa tgtaccgcat ccgcgatgac atgaaattcg
     1441 tttacgattt gctggtgcgc gaaaaagtct tgctggtgca gggaacgggg tttaattgga
     1501 tcaagcccga ccatttccgc attgttacgc tgccttacgt ctatcagatt gaagaggcga
     1561 tgggcaggct ggcaagattc ctgcaaacct accgccaata aggggacggt ttgtctgccg
     1621 aggataaaaa atgccgtctg aaacggagat tcccgtttca gacggcattt tcaacagcag
     1681 gaacgaatca ggcaaatttc agtctgtcgc cgtcggcttc caccctgatt tcgctttcgg
     1741 gcgcatagtt tccggcaagc agggcttttg ccagcgggtt ttcgatttcc gactggatgg
     1801 cgcgtttgag cggacgtgcg ccgtaaatcg ggtcgaaacc ggctttggcg atgatgtcca
     1861 gagcggcatc ggaaacagtc aggcggaggt tttgtttttc caaacgtttt tccaagcctt
     1921 tgagctggat tttcgcaatg ttgcggatat tatcctgatc cagtccgtgg aacacgacca
     1981 cttcgtcgat gcggttgatc atttcaggac ggaaatgttc tttcacatcc tccatcacaa
     2041 cttcttttac cgcttcgtaa tcctgaatgc ccatttgttg gatgtgctgg ctgccgatgt
     2101 tggaagtcat cacaatcacg gtatttttaa aatctaccgt cctgccttgc ccgtcggtca
     2161 aacgaccgtc atccaatact tgcagcagga tgttgaacac gtcgggatgg gctttttcca
     2221 cttcgtccaa aagaatcacg ctgtacggtt tgcggcggac ttgctcagtc aggtagccgc
     2281 cttcttcgta gccgacatag cccggaggcg cgccgattaa gcgggcaacg gcgtgttttt
     2341 ccatatattc ggacatatcg atacgaatca gatgatcttc gctgtcgaac agaaagcctg
     2401 ccagggcttt acacaactcg gttttaccca cgccggtcgg gcccaagaac aggaagctgc
     2461 cgtaaggctt gttcggatcg gcaagaccgg agcggctgcg gcggatagcg tcggacacgg
     2521 cacgcacggc ttcgtcctgt ccgaccacgc ggcggtgcaa tacttcttcc attttcagca
     2581 gtttgtcgcg ttcgccttcc atcattttgg atacgggaat gccggtcata cgggaaacca
     2641 cctctgcgat ttcctccgcg ccgacattat tacgcaagag tttgtttgcc ggttttgtgc
     2701 tgtccgtatc tgcccgttcg gcggctgcac gctgtttttc caaatgctcc aaatcttcat
     2761 acatcaattt tgaagccagt gccaaatcgc cttgccgttt tgcctgttcg attttaattt
     2821 tgacttcgtc aatttgtttc ttaatattag cagcaccgtc tgaaattgct ttttcggctt
     2881 tccagatttc gtccaaatcg gcatattctt tttccaaacc gtcgatttcc tcatctatca
     2941 gttccaaacg ttttttgctg gcatcgtctt tttctttttc aacgtgcgcc ttttccatcc
     3001 gaagctgaat tagacggcgg tcgattttgt ccattgcttc cggcttggtt tctttttcca
     3061 tcttgacacg gctggcggct tcgtcaatca aatcaatcgc tttatcaggc aggaagcggt
     3121 cggtaatgta gcggtcgctc aactccgctg cggcaacgat agcagggtcg gtaatatcga
     3181 taccgtggtg gatttcataa cgctcctgta aaccgcgcaa gatagcgatg gtgtcttcta
     3241 cacttggctc gccaaccaat actttttgga agcggcgttc gagtgccgcg tctttttcga
     3301 tgtattggcg gtattcgtcc aaggtggtcg cgccgataca gtgcaattcg ccacgtgcca
     3361 aagccggttt cagcatattg cccgcatcca tcgcaccgtc ggttttgcct gcgccgacca
     3421 aagtatggat ttcgtcgatg aaaatcagag tgttgccgtc gtctttcgcc aagtcgttca
     3481 acacgccttt caagcgttct tcaaattcgc cgcggtattt cgcgccggca atcaatgccg
     3541 ccaagtccaa aaccagtagg cgtttgttgc gcagggattc aggtacttcg ccattgacga
     3601 tgcgttgtgc caaaccttcg acgatggcgg ttttacccac gcccggttcg ccaatcagca
     3661 cagggttgtt tttggtacgg cgttgcaata cctgaatcgc acggcggatt tcgtcgtcac
     3721 ggccgataac ggggtcgagc ttaccgtctc gggcgcgttg ggtcagatcg agcgtatatt
     3781 ttttcaaagc atcgcgttgg tcttcggcat tggcatcgtt cacgttttgt cctcctcgta
     3841 ccgcgtcaat cgcggcattg atgttttgtt cggtcgcgcc ggcttctttc aaaattttgc
     3901 cggccgcatc gttctgctgc accaaggcaa gcaggaaaag ttcactggcg atataggcat
     3961 cgccgcgttt ggttgcggct ttgtccatca ggttcaacac cgcctgcaat tcgcggctgg
     4021 gcagaatgtc gccgccctgg ccggacactt tcggcaggct gttcaaatgc tgctgcaaac
     4081 gctgtttcac ctgcggcacg ttcacgcccg catgagccaa gagcgcggca gcaccgctgt
     4141 tttggtcgtc aagcagggct ttcagcacaa agcccgcttc cagatagctg ccgtccgcag
     4201 ccaacgccaa actctgagct tctgcaaggg cctgttggaa tttggcggtt aatttgtcgt
     4261 atcgcatttt tgtttccttt tcaaaatgtc cgctgtcgaa gcctatatgt gcataattgt
     4321 ggataactca agttctgttt tctgtttttc tatatttaat tcgatatatc attgaattta
     4381 aagtatataa aaatgtataa taatgtgtat aactatatct tcttaatatg gaaaagtctg
     4441 ttgtcggctg gatgtaggtg gcaaatcggg tataatcggc gcatcttttt ccctttcaga
     4501 cggcattgat gccgcaagga catttttatg agcaaaaaac gagttctgac cggcgtaacc
     4561 accaccggca tcccgcatct gggcaactac gtcggtgcta tccgccccgc cgtccgcgcg
     4621 gcgcaaaacc ctgataccga atccttcctc ttcctcgccg attaccacgg tatcatcaaa
     4681 tgccacgagc cggagatgat tcaccaatcc acccaagccg ttgccgccac ttggcttgcc
     4741 tgcggactcg accccgagcg caccaccttc taccgccaaa gcgacattcc cgaagtgatg
     4801 gaattgaact ggattctgac ctgcatcacc gccaagggtt tgatgaaccg cgcccacgcc
     4861 tacaaagccg ccgtgcaggc aaatgcggaa aacgggcagg aagaccccga tttcggcgtg
     4921 gaaatgggtt tgttcagtta tccgattctg atgactgccg atattctgat gttcaacgcc
     4981 aacgaagtcc ccgtcgggcg cgaccaaatc caacacgtcg aaatggcgcg cgacatcgcc
     5041 ggccgcttca atcaccgctt ccgagaactc ttcaccctgc ccgaagtgaa aatcgacgaa
     5101 aacgtcgaac tcttggtcgg tttggacgga cgcaaaatgt ccaaatccta cggcaacacc
     5161 attccgcttt gggaaaacga caaaaaaacc caaaaatcgg tcaacaaaat catcaccaat
     5221 atgaaagagc cgggcgagcc gaaacagccc gacgaaagcc cattgtttga aatctacaaa
     5281 gccttctcca cgccgtctga aacggtggaa tttacgaaaa tgcttgccga cggcctggct
     5341 tggggcgagg caaaaaaact tttggcggcg aaaatcaacg ccgaactcgc cgaaccgcgc
     5401 gaacgctaca acgagctgac cgccgaccct tcgcaaatcg aagagatttt acaggcaggt
     5461 gcgcaaaaag cgcgtaaaga agcacgcgaa ttgttggaca aagtacgcga tgcggtcggc
     5521 atccgcccgt tgaaataaac ccgatgccgt ccgaacctct taaaggcttg ccggaacaaa
     5581 acgtcgtccg gctgaccggc aagcatccca acgacttgga agccgtcagc ggcaaatgca
     5641 tggaaaccga cgataagggc ggtccggtag gctgggcgga aaacggcgtg tgccatacct
     5701 tgtttgccaa actggtgggc aatatcgccg aagacggcgg caaactgacg gattacctga
     5761 tttcgcattc cgccctgcaa ccctatcagg caggcaaaag cggctatgcc gccgtgcaga
     5821 acggacgcta tgtgctggaa atcgacagcg agggggcgtt ttatttccgc cgccgccatt
     5881 attgaggtat tcggacatcc cggaatatat ttgggttttt caaaccttgc gaaaaaaagt
     5941 ccgcaccgca gggaaactca aaaggagggg gatatgtgtt acaattttcc gaactgtttt
     6001 cataaaatag ttttcggacg tgtttcaata tggcattgat gccgccttat tgttcgagaa
     6061 aaaaaccttt atatttaaat ataatgggtt ttaactaaac gggaaaccgt tttctctccg
     6121 gccgatgggc aaaatcagcc gattgatgga acacggttcg atttttaagc aaaaccttaa
     6181 tcaacaactc aagaaggata agacatgaaa aaattattga ttgccgcaat gatggcggct
     6241 gccttggcag cttgttcgca agaagccaaa caggaggtta aggaagcggt tcaagccgtt
     6301 gagtccgatg ttaaagacac tgcggcttct gccgccgagt ctgccgcttc tgccgtcgaa
     6361 gaagcgaaag accaagtcaa agatgctgcg gctgatgcaa aggcaagtgc cgaggaagct
     6421 gtaactgaag ccaaa
//
LOCUS       DAWHRA010000115         6366 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWHRA010000115 DAWHRA010000000
VERSION     DAWHRA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.115"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..131)
                     /locus_tag="WIB30_001680"
     CDS             complement(<1..131)
                     /locus_tag="WIB30_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ3797715.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGISGAVALVEPLINTVV"
     gene            253..1206
                     /locus_tag="WIB30_001681"
     CDS             253..1206
                     /locus_tag="WIB30_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ3797716.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHASAVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1281..3338)
                     /gene="metG"
                     /locus_tag="WIB30_001682"
     CDS             complement(1281..3338)
                     /gene="metG"
                     /locus_tag="WIB30_001682"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ3797717.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKAGGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CGRIGIDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRDTLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAVPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     AFYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(3454..5292)
                     /gene="glmS"
                     /locus_tag="WIB30_001683"
     CDS             complement(3454..5292)
                     /gene="glmS"
                     /locus_tag="WIB30_001683"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ3797718.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GSFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            5574..6104
                     /locus_tag="WIB30_001684"
     CDS             5574..6104
                     /locus_tag="WIB30_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797719.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKKE
                     KGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTVQAYRDARNALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGRPGHCCFSGG"
BASE COUNT         1357 a         1520 c         1929 g         1560 t
ORIGIN      
        1 aaaacgacgg tgttgattag gggttcgacc agcgcgaccg ccccggatat gcctatactg
       61 cccgtcagta cataggttac actgaaggcg gtgctgaaat gcagtgcggc aaaagtcagg
      121 gttttaagca tcatcctctc ccgaattgga cattgacgga ggaatgataa aggttatcat
      181 aaggttgcgc ggtttaaatt tgctatttat tgttagcgta aataaatcat tttccaaata
      241 aggatagaaa ttatgaatca taaaaagatc gttgttttgg atgcggatac tttgcccggt
      301 cgggtctttc attttgattt tccgcacgag cttgcggttt acggtacgac aggtgcggat
      361 gaaacggcgg aacgggtgcg cgacgcacat attgtcatta ccaacaaagt gatgatttct
      421 gccgatatta ttgcggctaa tccgcagttg gagctgattg ccgtcagtgc gaccggtgtg
      481 aacaatgtcg atattggggc ggcgaaggcg gcgggcgttg cggtatgcaa tgtccgcgcc
      541 tacggaaacg aatcggttgc ggaacacgcc tttatgctga tgattgcgtt gatgcggaat
      601 ttgcccgcct atcagcgtga tgttgcggca ggattatggg aaaagtcgcc gtttttctgc
      661 cattacggtg cgccgattcg ggatttgaac ggcaaaacgc tggcggtttt cggacgcggc
      721 aatatcggac ggacgcttgc cggatacgcg caggcattcg gtatgggggt ggtgtttgcc
      781 gaacacaaac acgcgtccgc tgtgcgtgaa ggctatgttt cctttgaaga tgcggtacgg
      841 gctgctgatg tgttgtcgct gcactgtccg ctaaacgccc aaactgaaaa tatgataggc
      901 gaaaacgaat tgcggcagat gaagcctggc gcggttttaa tcaattgtgg gcgcggcggg
      961 ctggtggatg aaaacgcgct gcttgccgca ctcaaatacg ggcagatcgg cggggctggt
     1021 gtcgatgttt tgacggagga gccgccaagg ggcggcaatc ccctgctgaa tgcacgactt
     1081 cccaatctga ttgttacgcc gcataccgcg tgggcgagcc gcgaggcttt ggacaggctg
     1141 ttcgatatat tgttggcgaa cattcacgcc tttgtgaaag gcgaggcgca aaaccgggta
     1201 gtttgagctt gtcgggataa cgaaaaacaa tgccgtctga acgcccaaag ggttcagacg
     1261 gcattttttg agatttctac ttaaccgact ttgtcgcccg gttgcgcgcc tgcatccaca
     1321 tccaagagct tcagtttccc gtctgccgtg gcggcactca aaatcatgcc ttcggatacg
     1381 ccgaattttg ccattttgcg cggggcgaag ttggcgacgg cgatgaccat tcttccgttt
     1441 aactcggcag ggttggggta aaacgcggcg atgccggaga agatgatacg tttttcaaaa
     1501 ccgaaatcga ggtcgaattt taagagtttg gtgctgcctt cgacggcttc gcagttcaat
     1561 actttggcga cgcgcatgtc gattttcata aagtcgtcga aactcgcctg ttcggcaact
     1621 ttttcgtatt tgccctcttc ggcggcaggc acggctgcgg cggcgatgct ttgtttgttg
     1681 gcttcgatta aatcgtccac ttgtttttgc tccactcgtt gcatgagatg ttcgtacttg
     1741 ttgatggcgt gttcgcccaa ggtatcgcgt gtatttgccc aagtgatggc ttccaaattc
     1801 aggaatttgg cggcgtttgc ggcggtttgc ggcaagacgg gggcgaggta ggcggtcaac
     1861 atggtgaagg cgttgatgag ctcgctgcac acttcgtgca ggcgttcgtc ttgaccttcc
     1921 tgtttcgcca gttcccaagg tttgttggca tcaacgtatt cgttcaccgc atcagccaaa
     1981 gccataatgt cgcgcagggc gcgggcgtat tcgcggtttt cgtattgctc ggcgatggtg
     2041 tcgctttcgg ctgcaagttt tgccagcaat gcgctgtcgg caacgtcttt cagacggcct
     2101 tcaaagcgtt tggcgataaa acctgaggcg cgggcggcga tgttgacgta tttgcctacg
     2161 aggtcgctgt tgacgcgcga gataaagtct tgcaggttca aatcgatgtc ttcgattttg
     2221 ctgttgagtt ttgcggcgat gtagtagcgc atccactcgg ggtttaaacc ttgttccaga
     2281 taggatttgg cggtgataaa cgtgccgcgc gatttggaca ttttttgtcc gtctacggtc
     2341 aaaaagccgt gtgcgtacac gccggtcggg gcgcggtggc cggagaaatg cagcatagcg
     2401 ggccagaaca gggcgtgaaa atagaggatg tctttgccga tgaagtggta catctcggtt
     2461 tggctgtcgg ctttgaagta ttcgtcgaaa tcgatgccga tgcggccgca caggttttta
     2521 aacgatgcca tgtagccgac gggcgcgtcc agccagacgt agaagtattt gcccggcgcg
     2581 tcggggattt caaaaccgaa atacggcgcg tcgcgggaaa tgtcccagtc ggacagcgtg
     2641 gtttcttcgc cttcgcccag ccattctttc attttgttga gggcttcggc ttgcagatgg
     2701 ggcttgccgt cgtgcgggtt gttgccggaa gtccatgctt tgaggaagtc ggcgcattcg
     2761 cccagtttga agaagaagtg ttcggattcg cgcaattcgg gtttcgcgcc ggaaacggcg
     2821 gaatacgggt tgatcagttc ggtcggggaa taggtcgtgc cgcagacttc gcagttgtcg
     2881 ccgtattggt cttgggcgtg gcatttgggg cattcgcctt tgacgaagcg gtcgggcagg
     2941 aacatttgct tttcaggatc gaaaagctgc tcgatgacgc ggctttcgat tttgccgccg
     3001 gctttcaacg cgcggtaaat gtcttgggaa aactgtttgt tttcagggga atgggtgctg
     3061 taataattgt cgtagccgat gaaaaagccg gtgaagtcgg cgaggtgttc ttcgcgcact
     3121 ttggcaatca tgtcttcggg cgcgatacct tgtttttgcg cggcaagcat tacgggcgtg
     3181 ccgtgggtgt cgtcggcgca gcagtagtgg cactcgtggc cgcgcagttt ttgaaagcgc
     3241 acccaaacgt cggtttggat gtgttcgacc atgtggccga ggtggatgct gccgttggca
     3301 tagggcaggg cggaggtaac taagattttg cgtgtcatat tgtgctttgc aaacaatggg
     3361 taaaggcgga ttataccgca aatcaaacgg ggaaatgccg tctgaagcct gaaaaatcag
     3421 gcttcagacg gcatttttgc caaccggcgg gatttattcg acggttacgg attttgccag
     3481 atttcgcggt ttgtccacat ccgtgcctcg ggcgagggcg gtgtggtagg cgagaagctg
     3541 cacggggata gtatgcacga cgggggacag ttcgccgaca tggcgcggcg cacggataac
     3601 gtgcacgcct tcggtggcat taaaattgct gtcgaggtcg gcaaagacga aaagttcgcc
     3661 gccgcgcgcg ccgacttcct gcatattggc tttgactttg tccaacaggc tgtcgttggg
     3721 cgcgatgacg acgacgggca tattttcgtc caccagggca agcggcccgt gtttcagctc
     3781 gccggcagga taggcttcgg cgtggatgta ggtgatttcc ttcagcttca acgcaccctc
     3841 gagggcaatc gggtaatgga tgccgcgccc taaaaacagc gcgctggttt tcttggcaaa
     3901 ctgttgcgcc caggcggcaa tttgcggttc gaggttcaaa gcgtgctgca cgctgccggg
     3961 aagctggcgg agttcttcgg tataacgtgc ttcatcttcg ccggatacca agccgcgcac
     4021 tttcgccagc gttaccgcca aaccgaacag cgcgaccagc tgcgtggtaa atgccttggt
     4081 cgaggcgacg ccgatttccg caccggcacg ggtataaagc acgaggctgc tttcgcgcgg
     4141 cagggcggat tccatcacgt tgcaaatgga gaggctgtgg cggtgtccca aagatttggc
     4201 gtatttcaaa gcctccatcg tgtccagcgt ttcgccggat tgggaaatgg taatgaccag
     4261 ttggtcggga tcggcaatca cgctgcggta tcggtattcg ctggcgattt cgacgtcagc
     4321 cgggattttg gcgatggatt cgagccagta ttttgcggtc agcgcggcgt aataggacgt
     4381 gccgcaggca aggattttga cgctgcggat gctttcaaac acgcttttgg cgtttttgcc
     4441 gaagttttcg gggatgaagc tgccgtcgag gaaaacttcc gccgtatccg caatcgcgcg
     4501 cggctgctcg tggatttcct tttgcataaa gtggctgtac ggtcccagtt ccaaagaggc
     4561 gagcgagagt tcggatacct tgactttgcg ttcggcaggc aggccgtttt tatcggtcag
     4621 ccgtttgatg ccgtctgaag ccagcagcgc gatgtcgccg tcttcaaggt acgccacgcg
     4681 gcgcgtaaag gcgatgacgg cggatacgtc cgaagcgata aaggtttcat cgtcgcccaa
     4741 agcgaccaaa agcgggcagc ccatacgcgc cacaactaat tcatcaggct tgtcttgggc
     4801 aataaccgcg atggcgtatg cgccgtggaa acgtttgacc gcttcctgca cggcttcaaa
     4861 cagcctgccg ccgttttgcg cgtattcgtg attgatgctg tgtgcgatga cttcggtatc
     4921 cgtttgcgat tcaaaacggt atcccaaacc ttccaagcgt ttgcgttcgc tttcaaagtt
     4981 ttcgatgatg ccgttgtgga cgaccgcaat catcccgccg ctgatgtgcg ggtgggcgtt
     5041 cggctcggta acgccgccgt gtgtcgccca gcgcgtatgc ccgatgccga tgccgccgct
     5101 gatgcctttt tcgcgtgccg cgtcttccat aagctgcacg cgtccgacgc ggcgcacgcg
     5161 tttgattttg ccgtcggtgt tgacggcaat gcctgatgag tcataacccc ggtattcgag
     5221 gcgtttgagg ccgtcggtca gaaaatcgac gacgttgtga tgggcgcgga tggcgccgac
     5281 gataccgcac ataactgttc cttagtatcc ggttgaaaaa aaacaggcgc ggacggctcc
     5341 cgtgccgcac cttcctcttc ggattataaa ccgcctcccg cgccgaaaaa cagcaaaatg
     5401 ccgtctgaag gcttgggctt gctcaaaaaa gggagggatt tccccgcctg accgggatgg
     5461 gcgttcagac ggcattgcct gccgccggtt tatagttttt acaaatcaat attgacaagc
     5521 tgaaaaaaaa aaacaatata ctcgctcggt cttaatgtta acggagtata gaaatgaaac
     5581 aaatgctttt agccgtcggc gtggtggcgg tgttggcggg ctgcggcaag gatgccggcg
     5641 gttacgaggg ttattggcgc gaaaagtcgg acaaaaaaga gggcgtgatt gccgtcaaaa
     5701 aagaaaaagg caattacttc cttaataaaa tcaacgtatt tacaggcaag gaggagtctt
     5761 tgcttttgtc tgaaaaagac ggcgcgcttt cgataaacac agggataggg gaaatcccga
     5821 tcaaactttc cgacgacggg aaagagctgt atgtcgaacg caggcagtat gtgaaaaccg
     5881 atgcggcgat gaaggacaaa atcatcgccc accagaaaaa gtgcggacaa acagtacagg
     5941 cataccgcga cgcgcgaaat gcgttgccgt caaaccaaac gtatcagcag cgtcaggcgg
     6001 cgatcgagca attgaaacgg cggtttgaag ccgagttcga tgaattggaa aaagaaatca
     6061 aatgcaacgg cagacccggg cattgttgct ttagcggagg ataaccggga ggatgccgcc
     6121 gtccgaatcg gacgtgcggt ttccgtaccg gtacaggcgg acgggaatgt ccgccttttt
     6181 ttgttcggac gcgtttgaat atccgtccga ttccgtccgt ttgcaaaggg tatttttgtt
     6241 cgggcggaaa ttatagtgga ttaacaaaaa ccagtacggc gttgcctcgc cttagctcaa
     6301 agagaacgat tctctaaggt gctgaagcac caagtgaatc tgttccgtac tatttgtact
     6361 gtctgc
//
LOCUS       DAWHRA010000116         6287 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWHRA010000116 DAWHRA010000000
VERSION     DAWHRA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.116"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(145..1062)
                     /gene="thrB"
                     /locus_tag="WIB30_001685"
     CDS             complement(145..1062)
                     /gene="thrB"
                     /locus_tag="WIB30_001685"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ3797720.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPMAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSTEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1099..1827)
                     /gene="ubiG"
                     /locus_tag="WIB30_001686"
     CDS             complement(1099..1827)
                     /gene="ubiG"
                     /locus_tag="WIB30_001686"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ3797721.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVVETKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1979..3220
                     /locus_tag="WIB30_001687"
     CDS             1979..3220
                     /locus_tag="WIB30_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ3797722.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFAGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3353..3916
                     /gene="gmhB"
                     /locus_tag="WIB30_001688"
     CDS             3353..3916
                     /gene="gmhB"
                     /locus_tag="WIB30_001688"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ3797723.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            3945..4688
                     /locus_tag="WIB30_001689"
     CDS             3945..4688
                     /locus_tag="WIB30_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ3797724.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLALKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            4685..5377
                     /locus_tag="WIB30_001690"
     CDS             4685..5377
                     /locus_tag="WIB30_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ3797725.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLAPTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTGIRLNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYADEAKQW
                     LKDRGRELFALG"
BASE COUNT         1468 a         2003 c         1488 g         1328 t
ORIGIN      
        1 cggcttggtc gccttgtcct gatttttgtt aatccactat aaaatggaaa atacccggct
       61 atcgtctcat tttcgtttta atcagccata aaaatgccgt ctgaaaccct ttcagacggc
      121 atttctgtca aacgccggac gcactcaatc caaactcaac agcaggttgc ggaacgcgtt
      181 cgggtctttg ataaacgtca tctcgcccgc ctgcggaaaa tgaaagtcca acaggcgcga
      241 cacccaaaaa cggatgcagc cggcacgttg ggcggtcggg aaatacgcct tttcttcggt
      301 actcaagggg cgcacgccct cataaccgcc gataaacgcc tttttcaacg cctcatccaa
      361 cttattgtcc gccgtccttg cccaatcgtt gaccgcaatc gccaagtcat acataaaatt
      421 gccccggcag gcataataaa aatcgatgaa gcccgatacc tgaccgccgt caagcaacac
      481 attgtcttta aacaaatcgg cgtggatgat gcccgaagga agatgattgc cgagattgtc
      541 cttcaacgca tcgatttcgg aacacagcag cgcggcatcg tcttgcgaca ggacgggcag
      601 cagccgggcg cacgcctccg tccaccacgc atcgtaacgc gggttttcca tttccaaagg
      661 gaaatcggcg gcggcaaggt gcattttcgc caacatcgca ccggtatgga agcactgttc
      721 cgccatcggc agcgcggtat ccgaaccttt caggcaggca accaaacaag ccggcttgcc
      781 cgccaaaacg gaatcaagcc ggccgtcttt gcgcgcaacc ggcgcggcaa ccgccacgcc
      841 cttcatactc aaatgccggt taagctccag aaaaaacggc agctcttcct gtttcaacac
      901 ttcaaacacg gtcagcacat aacgtcccga agtcgtcgtc agaaaataat tgctgttggt
      961 aatcccctgc gcgatgccct gcagggaaac aaattccccc aaatcgtaac cgctcaggaa
     1021 gccgcgcatt tcatcatcgg aaacactggt atagacagac atatcaactc acttgctcaa
     1081 aaacgggcaa acccgccgtc agaacgccgg acggcaggca aacatataat tcacatcggt
     1141 cgaatcgcac agggcataag tttgcgacaa tacatggtaa gtcataccct tggtttccac
     1201 cacgtccaaa cccgcctgac ggcacattcg cgccagctcg gcaggtgcga tgaatttttt
     1261 ccagtcgtgc gtgcctttgg ggacaaactt caacagatat tccgccgcca caatcagatg
     1321 caggtacgat ttcgggtttt tattgatggt ggaaaaaaac accatgccgt ccggtttgac
     1381 cagcttggca caggcgcgaa cgatggcggc gggatcgggg acgtgttcca tcatttccat
     1441 gcacgttacc acatcgaacg agtgcggttc cgcctcggca aggtcttcca cgcggataca
     1501 ttcgtattcg atatcggcga cattgttcaa agccgcgtgc aggcgggcgg tttccagcga
     1561 ctgctccgcc atgtcgatac cctttacaaa cgccgcgccg cgccgcgcca tactttccgc
     1621 caagatgccg ccgccgcagc ccacatccaa aacccgtttg ccgcgcaaat ccgcgtgtcc
     1681 gtcgatataa tccagccgca gcggattgat gtcgtgcaag gttttgaact cgcccgactt
     1741 gtcccaccat ttgtcggcaa tccggctgaa tttggcgatt tccccctcat cgacattata
     1801 ttttttgtcg gacattttcc ctcccatctg acgaaccgcc cactccaaaa cccaagatta
     1861 tactatggaa cacaatgccg tctgaaagcc ttttcgggcg gcgcggcgca aatgccttac
     1921 aaatccttac actttacggc ataatggcgg ctcgcttttt ctggcagaaa gacaaaatat
     1981 gcccaacaaa accccttcac tgttcggcgg cgcgatgatt atcgccggca cggtcatcgg
     2041 cgcaggcatg ctcgccaacc cgaccgccac atccggcgta tggtttgccg gctcgctggc
     2101 cgtgttgctg tacacctggt tttctatgct ttccagcggt ctgatgattt tggaagtcaa
     2161 cacccactac ccccacggcg caagtttcga cacgatggtc aaagacctgc tcggacgcgg
     2221 ctggaacatc atcaacggca tcgccgtcgc cttcgtttta tacctgctca cctacgccta
     2281 catcttcgtt ggcggcgacc tgaccgccaa aggcttaggc agcgccttgg gcggcaatgt
     2341 ttcactcacc gtcggacaac tcgtcttctt cggcatcctc gccttttgcg tatgggcatc
     2401 cgcacgcttg gtcgaccgct tcaccagcgt cctcatcggc ggcatggtat taacctttat
     2461 ttgggcaacc ggcggcctga ttgccgatgc caaaatgccc gtcctgttcg acacccaagc
     2521 ccccgtcggc accaactact ggatttacgc cgccaccgcc ctgcccgtct gccttgcgtc
     2581 attcggtttc cacggcaacg tctccagcct gctcaaatac tttaaaggcg acgcgcccaa
     2641 agtggctaaa tccatctgga cgggcacact gattgcgctg gtaatttacg tcctctggca
     2701 aaccgccatc caaggcaacc tgccgcgcaa cgagttcgcc cccgtgattg ccgccgaagg
     2761 gcaagtctcc gtcctcatcg aaaccctgtc caaattcgcc caaaccggca atatggataa
     2821 aatattgtcc ctgttttcct atatggcgat cgccacctcg tttttaggcg taaccttagg
     2881 tctgttcgac tacatcgccg acatcttcaa atggaacgac agcgtgtccg gccgcaccaa
     2941 aaccgccgcg ctgaccttcc tgccgcctct aatttcctgc ctgctcttcc ccaccggctt
     3001 cgttaccgcc atcggctacg tcggcctggc ggcaaccgtc tggacaggca tcatccccgc
     3061 catgctgctc taccgttcgc gcaaaaaatt cggcgcaggc aaaacctata aagtttacgg
     3121 cggcttgtgg ctgatggttt gggtcttcct tttcggcatc gtcaacatcg ccgcacaggt
     3181 attgagccaa atggaactcg tccccgtatt taaaggataa aggcaaaatg ccgtttgaag
     3241 cccgccggcg gcttcagacg gcattgcctc gttatcacgc ggcacaggcg catcactgcc
     3301 gatatttcag tataatgcca cccccgacct gccccaatcc aaaggaaacg cgatgaaact
     3361 catcattctc gaccgcgacg gcgtcatcaa tcaggaccgc gacgacttcg tcaaatccgt
     3421 tgacgagtgg atacctgtcg aaggcagcat ggatgcggtg gcatttctga cgcaggcagg
     3481 ctacaccgtc gccgttgcca ccaaccaatc cggcatcggg cgcaaatatt ttaccgttca
     3541 aaaccttacc gaaatgcacg ccaaaatgca ccgcctcgtc cgtcaggcag gcggcgaaat
     3601 caacggcatc tggttctgcc cgcacaccga tgccgacaac tgcaactgcc gcaagcccaa
     3661 accgggtatg attgaagaca tcatcggacg cttcaacgcc caagcctcgg aaacctggct
     3721 ggtcggcgac agcctgcgcg atttgcaggc aatcgatgcc gtcggcggca aacccgcgct
     3781 ggttctgacc ggaaaaggca aaaaaacgct ctcccaacac ggacacgaat tgcccgaaca
     3841 cacacaggtt ttcgataccc tgctcgattt ctcacaatac atcatgcagg aaaacaccgc
     3901 accgcaagcc gactgaacat accgcattcc gacaaggcaa aaccatgctc atcatccgca
     3961 acctgattta ctggctgata ctctgttcca gcctgatttt cctctttccc tttatgctgc
     4021 tcgcctcgcc tttccgggac ggggcgcaca agatggcgcg ggtctgggtc aaaatcctca
     4081 acctctcgct caaacacatc gtcgggctca aataccgcat catcggcgcg gaaaacatcc
     4141 ccgaccgccc cgccgtcatc tgcgccaaac accaaagcgg ctgggaaacg cttgcccttc
     4201 aggacatttt tccgccgcag gtttacgttg ccaaacgcga gctgttcaaa atcccctttt
     4261 tcggctgggg tttaaaactg gtcaaaacca taggcataga ccgcaacaac cgccgcgaag
     4321 ccaacgaaca gctcataaaa caggggttgg cgctcaaaaa cgaaggctat tggattacca
     4381 ttttccccga aggcacgcgc cttgcgcccg gaaaacgcgg caaatacaaa ctcggcggcg
     4441 cgcgcatggc gaaaatgttt gagatggaca tcgtccccgt cgccctcaac agcggcgaat
     4501 tttggccgaa aaactccttt ctgaaatatc cgggggaaat caccgtcgtc atctgtccga
     4561 ccatcccgca cgcaagcggc agcgaagccg aattgatgga aaaatgcgaa cacctcatcg
     4621 aaacgcagca gccgctcatt tccggcgcag gtccgtttgc cgccaaaatg ccgtctgaaa
     4681 ccgcatgacc gcctttgtcc acaccctttc agacggcatg gaactgaccg tcgaaatcaa
     4741 gcgccgtgcc aagaaaaacc tgattatccg ccccgccggc acacataccg tccgcatcag
     4801 cgtcccaccc tgcttctccg tctccgctct aaaccgctgg ctgtatgaaa acgaagccgt
     4861 cctgcggcaa acactggcga aaacaccgcc gccgcaaact gccgaaaacc ggctgcccga
     4921 atccatcctc ttccacggca gacagcttgc ccctaccgcc catcaagaca cgcaaatcct
     4981 gctgatgccg tctgaaatcc gtgttcccga aggcgcaccc gaaaaacagc ttgcgctgct
     5041 gcgggacttt ttggaacggc aggcgcacag ttacctgatt ccccgcctcg aacgccacgc
     5101 ccgcaccaca caactgttcc ccgcctcctc ctcgctgacc tctgccaaaa ccttctgggg
     5161 cgtgtgccgc aaaaccacag gcatacgcct caactggcgg ctggtcggcg caccggaata
     5221 cgttgccgat tatgtctgca tacacgaact ctgccacctc gcccatcccg accacagccc
     5281 cgccttttgg gaactgaccc gccgcttcgc cccctacgcg gacgaagcca aacaatggct
     5341 caaagaccgc ggcagggaac ttttcgcctt aggctgacgc ggaccggacc gacccgccgc
     5401 tttcagacgg catccgtgcc ggaacaggca cgcgcccgcc cgattcaaac cgcgatgacg
     5461 ctttgccgcc ggttcggggc aggatggcgg cacacgcgcc gtctgccgcg tttcatttca
     5521 caccgctctt ccgaaacccg aaacccgccc ggtccgacgt gcggtatgaa acgcttaagc
     5581 tgacgcgaag tcttttactg atttgcccgc gaaaatgccg tctgaaaggt tttcggacgg
     5641 catttttttg cgtttcccag gatggcggcg gattcgtaaa aggcggtcag ggtggattgt
     5701 aggatgggtt gagacctgcc gaatccgccg catctgccaa atctaccgcc gtcattccta
     5761 cgaaagtggg aatctagaac gcggggttaa gaaaacctgc accgcgtcat tcccacgaaa
     5821 gtgagaatcc aggacgcaaa atctcaagaa accttttttc ccgataaatt tccgcaccga
     5881 cagacctaga ttcccgcctg cgcgggaatg acgggatttg aggtttctgt tcgcgtcatt
     5941 cccacgaaag tgggaatcca gttttttgag tttcagtcat ttccgataaa ttgccttagc
     6001 attgaatgtc tagattcccg actgcgcggg aatgacgaat ccatccatac ggaaacctac
     6061 atcccgtcat tcctacgaac ctacattccg tcattcccac gaaagtggga atccagaatc
     6121 ccagactttc agataatctt tgaatattgc tgttgttcta aggtctagat tcccgcctgc
     6181 gcgggaatga cgggatttga ggtttctgtt cgcgtcattc ccacgaacct gcatcccgtc
     6241 attcccacga aagtgggaat ctagttttgt cggtgcggaa acttatc
//
LOCUS       DAWHRA010000117         6281 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWHRA010000117 DAWHRA010000000
VERSION     DAWHRA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.117"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1..184)
                     /locus_tag="WIB30_001691"
                     /pseudo
     CDS             complement(1..184)
                     /locus_tag="WIB30_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            complement(1399..2715)
                     /locus_tag="WIB30_001692"
     CDS             complement(1399..2715)
                     /locus_tag="WIB30_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ3797726.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTRSHKIDQKEFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDGEGNWSDVGLSSASFLGGLMGVQWGAALGVGAFGLLTGGAGWLVVGA
                     ILGGGAGAWWGSLGAEKAWRWFEDFLNLNRSDKYHVYDPLALDLDGDGIETVATKGFS
                     GALFDHRNQGIRTATGWVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGY
                     AALAELDSNGDNIINAADAAFQSLRVWQDLNQDGISQTNELRTLEELGIQSLDLAYKD
                     VNKNLGKESKPQSAVLAIHTPFVCFLGNLKGIICTRHNPILYFTKN"
     gene            3991..6123
                     /locus_tag="WIB30_001693"
     CDS             3991..6123
                     /locus_tag="WIB30_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ3797727.1"
                     /translation="MPTVPTPLPALSALIILAHYHGIAANPADIQHEFCASAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPVKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
BASE COUNT         1747 a         1300 c         1334 g         1900 t
ORIGIN      
        1 cgttatggtc gaagaggctg cctgaaaagc ctttggcggc gactgtttct atgccgtcgc
       61 cgtctagatc tagggcgagg gggtcgtaaa cgtggtattt gcctgaacga tttctgtcta
      121 attctttccg tagattccaa tcatcaggta gccagtcata taccctatga gaccagtctt
      181 caaatttctc cttcaggaga ctgccaatgc tttcagaatc aaaagaaata tttctatctg
      241 ttaaattatg aaatgaattc tgaatctcct taaatttatt cctgatatta tttaatgttt
      301 cttttaatga attttctaaa gaatcattaa atgtaccaag gttttttccc caatcatatc
      361 cattttctat atttggaaca ttaggcgggt tgttatagta tttcccctca gtattgatat
      421 tactattaga taacgaattc cttaaattta ccgctccacc tccagcaatg caaatatcct
      481 tagaaacacc aattgcacca gcacaagcta ctccaacatc aaaagatgca atgggctgaa
      541 acgctggcac aaatccccca tacgattttc catggaatga acgctgcaaa tctttttctt
      601 tacctgtacc atagtttagt agattaagtg gattctccgc tccataagcc catgcttcat
      661 gagggttgta tccgacggga gcaatataag gattaccatt cttatctttt agataaccac
      721 cagctccatc tgaaacatac gaagctttaa ctgtaccaat aatattttgc ttaggatctt
      781 ttggattgta ttttgtatat acatttacct caaaaatttc cataactgag cctttacttg
      841 ttgataaaag ataaaataaa attagaaact aaaagtgaga aaaagttaat aataataggg
      901 atgtataaat gtaaaggttc tgtttcatag ctaaggttat ctgaatatat ggaaaaaaag
      961 taaaagtcca taaaagtaaa atatatagat aatgataatg ataatagaat tatcactttc
     1021 ctagagtaag gataatattt ttttacgata aaaaaatata aaataggata aataattgcc
     1081 atatagatag attttgtagc aaaattaaaa ataaataata aaaataatgt aataaagtat
     1141 attttttggc tatgaaataa aattgtacat aattgaacga gcagatcaaa aaatgaacta
     1201 catataacaa taaataataa cgtatttacc atactaaatt taataggtct cattatcata
     1261 tttaataacc acttcatagt aaatgcagag tgggtggaaa cactcacttt atggtttgct
     1321 acgctctgct caattagcaa cccgataacc caatatagat aatagggcaa ttaatccaat
     1381 ctaatttgtt aaaatacgtt aattttttgt aaaataaagt attggattat gtcgggtgca
     1441 aatgatgcct tttagattgc ctaaaaaaca aacaaacggt gtgtgtatag caagtactgc
     1501 actttgcggc ttactttctt taccgagatt tttatttaca tctttatagg cgagatccaa
     1561 agattgaata cccaattctt caagggtgcg gagttcgttg gtttgggaaa tgccgtcctg
     1621 attgagatcc tgccatacac gcagggattg gaatgcggcg tctgccgcgt tgatgatgtt
     1681 gtcgccgttt gaatccaatt cggccaaagc cgcgtagccg tgtttggcaa aagaaccgtc
     1741 tgccagtttg gtattgtcgc cgaagagttc cgcaccgttg tcgatgatgc cgttgccgtt
     1801 caaatcgcgc acaagcagac cgtcatcggc agaaacccaa ccggtggcgg tgcggatgcc
     1861 ttggttgcgg tggtcgaaca atgcacctga aaagcctttg gtggcaacgg tttctatacc
     1921 gtcgccgtct agatctaggg cgagggggtc gtaaacgtgg tatttgtcgc tgcggttaag
     1981 atttaagaaa tcctcaaacc atctccaagc tttttcagct cctaatgatc cccaccaagc
     2041 tcctgcgcca cctccaagta tagctccaac tactagccaa cctgcgccac cagtaagtaa
     2101 accaaaagcc ccaacaccca aagccgcacc ccattggacg cccattagcc cacccaaaaa
     2161 agaagctgat gagagaccaa catcactcca atttccttct ccatccaagg ctttccacca
     2221 agcacttccc atactcaaag ttcccatggc aacagttgcc ccataagtaa acttacccaa
     2281 attcgctgcc ataacttttg attttgaagc attctctaac gctgttgccg cacgtcttgc
     2341 atattgctgc ttgttatagt tgctagcatt caatgctttt tgcaaatcac tttgtacttt
     2401 tcttgcaaca aatgcctcct ttgaattacc acgaaattta ctagcagctc ttccaattcc
     2461 ttcttttaag cttttttgtg cctcttctgc tgtgcgttgg aattctcttg ccttagcaga
     2521 cagacgctca ccctccctta aaaattcttt ttgatctatc ttgtgcgaac gggtcaaata
     2581 ttcttcgtac attgagttaa tcgtaccaat cgccctaacc acattttcat cagaaaatat
     2641 ggaaataata gcatccctat acgcacctag tgtaatattg tttctattat tagttatagc
     2701 attattcgaa tacataatag cacctccaaa tttagaactg aatacgttta tattctctgt
     2761 gtaataatat caaataatta attaatggat aagtaagtcc gaaaaaataa caactaaaat
     2821 aagcaatata ctgattctct cctttattaa ttagatatat tgaaaaacaa ataaaaaata
     2881 aacagaaaac attcatgtga aaaagccacc tcatattctt atttataagt ttaaaataac
     2941 taggcattgt atatttaaaa atcggtacat ccaacggaaa caaaccacat aaaaattttt
     3001 ctttattctt tattaaagac atagttttat ttttcaaaat tagtccagat aatatactaa
     3061 caattactat taaaacaata agcacagcgt aattttttat aaaattcatt aaaataataa
     3121 tgaaaatact aaatagatta catactataa attgaaatat aaaatattca gtatttaatc
     3181 cattttcaac cgcgagtttt ttaaaatatc cttgctcgaa aaaattaaaa aaatccatta
     3241 atactttcct ctcaagaaaa ctaattttag tttagttaag ataaaatgca atattacctt
     3301 ttctccataa aagtatggag cttatataca taaattatgt gcgaggtagc ctgaaaaata
     3361 tccaatacaa gatagtagga gatgtttccc ttatcactat tggccaatac ccaattggca
     3421 ggcactctat ttccaaaatt ttctcaagaa tagttaaata ctattaatta gtttaaaaac
     3481 aataacatac gtttgtattt atccgttttg gtctgcctga aacagactct atgtccatac
     3541 acggacatgt agacagtttc cttgttattt tggttgctcg tcattagtga tgatacttta
     3601 ctcctctctt aaaaaatcgt caagaactgt tatcaatctt taaagtcttt tttaaacaaa
     3661 tatatatacg ttcaggctgt ctgaaacaca aaaaaatgat gttacccgac cgtgacagag
     3721 tcggggcagc cggtacctta accctgcacc ttaaccctgt gccttaacgg tatataggag
     3781 gcacgatagg tgcgaggcat ccgcatctat cttgattcct aagccgtttt accttcctga
     3841 aacgagaact tttccatact ttccctttac aattcctgta tagatcctta caatgtgttc
     3901 tgctgtttta tttatatatt tctttctact tagatatatt ctttttccag acagccgtta
     3961 tccacatcca atctaacata tattttattt atgcctaccg tacccacacc tctccccgcc
     4021 ctttccgccc ttattattct tgcgcattac cacggcattg ccgccaatcc tgccgatata
     4081 cagcatgaat tttgtgcttc cgcacaaagt gatttaaatg aaacgcaatg gcttttagct
     4141 gccaaatctt tgggattgaa ggcaaaggta gtccgccagc ctgtcaaacg tttggctatg
     4201 gcgaccttac ctgcattggt atggtgtgat gacggcaacc attttatttt ggccaaaaca
     4261 gacggtgggg gtgagcatgc ccaatatcta atacaggatt taactacgaa taagtctgcg
     4321 gtattgtctt ttgccgaatt ttctaacaga tattcgggca aactgatatt ggttgcttcc
     4381 cgcgcttcgg tattgggcag tttggcaaag tttgacttta cctggtttat tccggcggta
     4441 atcaaatacc gccggttgtt ttttgaagta ttggtggtgt cggtggtgtt gcagctgttt
     4501 gcgctgatta cgcctctgtt tttccaagtg gtgatggaca aggtgctggt acatcgggga
     4561 ttctctactt tggatgtggt gtcggtggct ttgttggtgg tgtcgctgtt tgagattgtg
     4621 ttgggcggtt tgcggacgta tctgtttgca catacgactt cacgtattga tgtggaattg
     4681 ggcgcgcgtt tgttccggca tctgctttcc ctgcctttat cctatttcga gcacagacga
     4741 gtgggtgata cggtggctcg ggtgcgggaa ttggagcaga ttcgcaattt cttgaccggt
     4801 caggcgctga cttcggtgtt ggatttggcg ttttcgttta tctttctggc ggtgatgtgg
     4861 tattacagct ccactctgac ttgggtggta ttggcttcgt tgcctgccta tgcgttttgg
     4921 tcggcattta tcagtccgat actgcggacg cgtctgaacg ataagttcgc gcgcaatgca
     4981 gacaaccagt cgtttttagt agaaagcatc actgcggtgg gtacggtaaa ggcgatggcg
     5041 gtggagccgc agatgacgca gcgttgggac aatcagttgg cggcttatgt ggcttcggga
     5101 tttcgggtaa cgaagttggc ggtggtcggc cagcaggggg tgcagctgat tcagaagctg
     5161 gtgacggtgg cgacgttgtg gattggcgca cggctggtaa ttgagggcaa gctgacggtg
     5221 gggcagctga ttgcgtttaa tatgctctcg ggacaggtgg cggcgcctgt tatccgtttg
     5281 gcgcagttgt ggcaggattt ccagcaggtg gggatttcgg tggcgcgttt gggggatatt
     5341 ctgaatgcgc cgaccgagaa tgcgtcttcg catttggctt tgcccgatat ccggggggag
     5401 attacgttcg aacatgtcga tttccgctat aaggcggacg gcaggctgat tttgcaggat
     5461 ttgaacctgc ggattcgggc gggggaagtg ctggggattg tgggacgttc ggggtcgggc
     5521 aaatccacac tcaccaaatt ggtgcagcgt ctgtatgtac cggagcaggg acgggtgttg
     5581 gtggacggca acgatttggc tttggccgct cctgcctggc tgcggcggca ggtcggcgtg
     5641 gtcttgcagg agaatgtgct gctcaaccgc agcatacgcg acaatatcgc gctgacggat
     5701 acgggtatgc cgctggaacg cattatcgaa gcagccaaac tggcgggcgc acacgagttt
     5761 attatggagc tgccggaagg ctacggcacc gtggtgggcg aacaaggggc cggcttgtcg
     5821 ggcggacagc ggcagcgtat tgcgattgcc cgcgcgttaa tcaccaatcc gcgcattctg
     5881 atttttgacg aagccaccag cgcgctggat tatgaaagtg aacgagcgat tatgcagaac
     5941 atgcaggcca tttgcgccaa ccggacggtg ctgattatcg cccaccgtct gtccaccgtt
     6001 aaaacggcac accgaatcat tgccatggat aaaggcagga ttgtggaagc gggaacacag
     6061 caggaattgc tggcgaagcc gaacggatat taccgctatc tgtatgattt acagaacggg
     6121 tagaaggagg ggatgggcgt gtaggctgct tttttcgtaa cgttctgcgt tttcagaccg
     6181 tcgtttactg ggtttactga ctgaaatgaa accaaagtgc aggctgcctg aaagactttt
     6241 tctgtctttt taggcagcct gcacttcttt ttcactcatc t
//
LOCUS       DAWHRA010000118         6258 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWHRA010000118 DAWHRA010000000
VERSION     DAWHRA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.118"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            377..1252
                     /gene="prpB"
                     /locus_tag="WIB30_001694"
     CDS             377..1252
                     /gene="prpB"
                     /locus_tag="WIB30_001694"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ3797728.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            1338..2492
                     /gene="prpC"
                     /locus_tag="WIB30_001695"
     CDS             1338..2492
                     /gene="prpC"
                     /locus_tag="WIB30_001695"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ3797729.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            2645..3433
                     /locus_tag="WIB30_001696"
     CDS             2645..3433
                     /locus_tag="WIB30_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ3797730.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFVGGVAGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            3511..6117
                     /gene="acnD"
                     /locus_tag="WIB30_001697"
     CDS             3511..6117
                     /gene="acnD"
                     /locus_tag="WIB30_001697"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ3797731.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGSVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGTLA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVIGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
BASE COUNT         1514 a         1616 c         1651 g         1477 t
ORIGIN      
        1 atggaattaa aataaaatat taaaattatg taatgagtct ccttaaaaat gtttgacatt
       61 ttcagtcttg tgttttagat tatcgaaaaa taaaactaca taacactaca aaggaacatt
      121 actatgaaac caattcagat gttttcccct tttctgaata atccccttgt tttcttcttg
      181 tctgcggttt tgccgcataa ttccgaacgg tctgctgttt ttctttgatt cgttttaaat
      241 atcaataaga taatttttcc catatatttt taatgattgg attgggatgc ccgacgcgtc
      301 ggatggctgt gttttgccgt ccgaatgtga tggaagcctg tccatactga aaaaaagtct
      361 ataaaggaga aatatgatga gtcaacactc tgccggagca cgtttccgcc aagccgtgaa
      421 agaatcgaat ccgcttgccg tcgccggttg cgtcaatgct tattttgcac gattggccac
      481 ccaaagcggt ttcaaagcca tctatctgtc cggcggcggc gtggcagcct gttcttgcgg
      541 tatccctgat ttgggcatta ccacaatgga agatgtgctg atcgacgcac gacgcattac
      601 ggacaacgtg gatacgcctc tgctggtgga catcgatgtg ggttggggcg gtgcattcaa
      661 tattgcccgt accattcgca actttgaacg cgccggtgtt gcagcggttc acatcgaaga
      721 tcaggtagcg caaaaacgct gcggccaccg tccgaacaaa gccattgtat ctaaagatga
      781 aatggtcgac cgtatcaaag ctgccgtaga tgcgcgcgtt gatgagaact tcgtgattat
      841 ggcgcgtacc gatgcgctgg cggtagaagg tttggatgcc gctatcgaac gcgcccaagc
      901 ttgtgtcgaa gccggtgcgg acatgatttt ccctgaagcc atgaccgatt tgaacatgta
      961 ccgccaattt gcagatgcgg tgaaagtgcc cgtgttggcg aacattaccg agtttggttc
     1021 cactccgctt tatacccaaa gcgagctggc tgaaaacggc gtgtcgctgg tgctgtatcc
     1081 gctgtcatcg ttccgtgcag caagcaaagc cgctctgaat gtttacgaag cgattatgcg
     1141 cgatggcact caggcggcgg tggtggacag tatgcaaacc cgtgccgagc tgtacgagca
     1201 tctgaactat catgccttcg agcaaaaact ggataaattg tttcaaaaat gatttaccgc
     1261 tttcagactg cctttcaaca aatccgcatc ggtcgtctga aaacccgaaa cccataaaaa
     1321 cacaaaggag aaataccatg actgaaacta ctcaaacccc gaccctcaaa cctaaaaaat
     1381 ccgttgcgct ttctggcgtt gcggccggta ataccgcttt gtgtaccgtt ggccgtaccg
     1441 gcaacgattt gagctatcgc ggttacgaca ttctggattt ggcacaaaaa tgcgagtttg
     1501 aagaagtcgc ccacctgctg attcacggcc atctgcccaa caaattcgag ctggccgctt
     1561 ataaaaccaa gctcaaatcc atgcgcggcc tgcctatccg tgtgattaaa gttttggaaa
     1621 gcctgcctgc acatacccat ccgatggacg taatgcgtac cggcgtatcc atgctgggct
     1681 gcgttcatcc tgaacgtgaa agccatccgg aaagtgaagc gcgcgacatc gccgacaaac
     1741 tgatcgccag cctcggcagc atcctcttgt actggtatca atattcgcac aacggcaaac
     1801 gcattgaggt tgaaagcgac gaagagacca tcggcggtca tttcctgcaa ctgttgcacg
     1861 gcaaacgccc aagcgaatca cacatcaaag ccatgcacgt ttcactgatt ctgtatgccg
     1921 aacacgagtt caacgcttct acctttaccg cccgcgtgat cgccggtaca ggctctgata
     1981 tgtactccag cattaccgga gcaatcggcg cgttgaaagg tccgaaacac ggcggcgcga
     2041 acgaagtggc ttacgatatt caaaaacgct accgcaatgc cgacgaagct gaagccgaca
     2101 tccgcgaacg catcggccgc aaagaaatcg tgatcggttt cggtcatccg gtgtacacca
     2161 tttccgaccc tcgcaacgtt gtcattaaag aagtggcacg cggtttgagc aaagaaaccg
     2221 gcgatatgcg cctctttgac attgccgaac gtttggaaag cgtgatgtgg gaagagaaaa
     2281 aaatgttccc gaatctggac tggttctctg ccgtttccta ccaaaaattg ggcgtaccga
     2341 ccgctatgtt cacaccgctg ttcgtaattt cccgtacaac cggttggagc gcacacgttc
     2401 ttgagcaacg caaagacggc aaaatcatcc gtccgagcgc aaactacaca ggccctgaag
     2461 atttggcgtt tgtggagatt gaagaacgat aattgaagaa tgcaatagca gtttgttctt
     2521 taatttcggt atgcaaagct aaggatttca gacgaccttg ccttattgga aaggttgtct
     2581 gaaataagtt taatctaata ggagaagata atcctgtatt ggcgcaagta acaggataag
     2641 aaacatggaa gatttatata taatactcgc tttgggtttg gttgcgatga ttgccggatt
     2701 tatcgatgcg attgcgggcg ggggtggttt gattacgctg cccgcactct tgttggcagg
     2761 tattcctccc gtgtcggcaa ttgccaccaa caagctgcaa gcagccgctg ctacgttttc
     2821 agctacggtt tcttttgcac gcaaaggttt gattgattgg aagaaaggtc tcccgattgc
     2881 cgcagcatcg tttgtaggcg gcgtggccgg tgcattatcg gtcagcttgg tttccaaaga
     2941 tattctgctg gcggtcgtgc cggttttgtt gatatttgtc gcactgtatt ttgtgttttc
     3001 gcccaagctc gacggcagta aggaaggcaa agccagaatg tctttttttc tgttcgggct
     3061 gacggtcgca ccgcttttgg gtttttacga cggtgtgttc ggaccgggtg tcggctcgtt
     3121 ttttctgatt gcctttattg ttttgctcgg ctgcaagctg ttgaacgcga tgtcttacac
     3181 caaattggcg aacgttgcct gcaatcttgg ttcgctatcg gtattcctgc tgcacggttc
     3241 gattattttc ccgattgcgg caacgatggc ggtcggtgcg tttgtcggtg cgaatttagg
     3301 tgcgagattt gccgtccgct tcggttcgaa gctgattaag ccgctgctga ttgtcatcag
     3361 catttcgatg gctgtgaaat tgttgataga cgagagaaat ccgctgtatc agatgattgt
     3421 ttcgatgttt taaacccttt cagacgaccc cttcaaaacg tcggctgaaa cctcaaacca
     3481 caagaaaaac agatccacag gagaaccgac atggctgcca accaacgtta ccgcaaaccg
     3541 ctgcccggta cggatttgga atactacgac gcgcgtgcgg cgtgtgagga catcaagccc
     3601 ggctcttacg acaagctgcc ttacacgagc cgcattttgg cggagaattt ggtcaaccgc
     3661 gcggacaaag tcgatttgcc gacgctgcaa agctggctgg ggcagttgat agaagggaag
     3721 caggaaatcg actttccgtg gtatccggcg cgggtggtgt gccacgatat tctggggcag
     3781 accgcgttgg tggatttggc aggcctgcgc gatgcgattg ccgaaaaagg cggcgatcct
     3841 gccaaagtga atccggtggt gcaaacccag ctcatcgtcg accactctct ggcggtggag
     3901 tgcggcggtt acgatcctga tgccttccgc aaaaaccgcg aaatcgaaga ccgccgtaac
     3961 gaagaccgtt tccacttcat caactggaca aaaaccgcgt ttgaaaatgt ggacgtgatt
     4021 ccggcgggca acggcatcat gcaccaaatc aatctagaaa aaatgtcgcc cgtcgtccaa
     4081 gtcaaaaacg gcgtggcttt ccccgatacc tgcgtcggta ctgactcaca tacgccgcac
     4141 gtcgattcat tgggcgtgat ttccgtgggc gtgggcggat tggaagcgga aaccgtaatg
     4201 ctgggacgcg cgtccatgat gcgcctgccc gatattgtcg gcgttgagct gaacggcaaa
     4261 cggcaggcgg gcattacggc gacggatatt gtgttggcac tgaccgagtt tctgcgcaaa
     4321 gaacgcgtgg tcggggcgtt tgtcgaattc ttcggcgagg gcgcgagaag cctgtctatc
     4381 ggcgaccgcg cgaccatttc caacatgacg ccggagttcg gcgcgactgc cgcgatgttc
     4441 gctattgatg agcaaaccat tgattatttg aaactgaccg gacgcgacga cgcgcaggtg
     4501 aaattggtgg aaacctacgc caaaaccgca ggcttgtggg cagatgcctt gaaaaccgcc
     4561 gtttatcctc gcgttttgaa atttgatttg agcagcgtaa cgcgcaatat ggcaggccca
     4621 agtaacccgc atgcccgttt tgcgaccgcc gatttggcgg cgaaagggct ggcgaagcct
     4681 tacgaagagc cttcggacgg ccaaatgccc gacggctcgg tcatcatcgc cgcgattacc
     4741 agttgcacca acacttccaa cccgcgcaac gttgttgccg ccgcgctctt ggcacgcaat
     4801 gccaaccgtc tcggcttgaa acgcaaacct tgggtgaaat cttcgtttgc cccgggttca
     4861 aaagtagccg aaatctattt gaaagaagcg ggcctgttgc ccgaaatgga aaaactcggc
     4921 ttcggtatcg tcgccttcgc ctgcaccacc tgcaacggca tgagtggcgc gctggatccg
     4981 aaaatccaga aagaaatcat cgaccgcgat ttgtacgcca ccgccgtatt atcaggcaac
     5041 cgcaacttcg acggccgtat ccacccgtat gcgaaacagg ctttcctcgc ttcgcctccg
     5101 ttggtcgttg cctacgcgct ggcaggcagt atccgtttcg atattgaaaa cgacgtactc
     5161 ggcgttgcag acggcaagga aatccgcctg aaagacattt ggcctgccga tgaagaaatc
     5221 gatgccgtcg ttgccgaata tgtgaaaccg cagcagttcc gcgatgtgta tgtaccgatg
     5281 ttcgacaccg gcacagcgca aaaagcaccc agtccgctgt acgattggcg tccgatgtcc
     5341 acctacatcc gccgtccgcc ttactgggaa ggcacgctgg caggggaacg cacattaaga
     5401 ggtatgcgtc cgctggcgat tttgcccgac aacatcacca ccgaccacct ctcgccgtcc
     5461 aatgcgattt tggccgtcag tgccgcaggc gagtatttgg cgaaaatggg tttgcctgaa
     5521 gaagacttca actcttacgc aacccaccgc ggcgaccact tgaccgccca acgcgctacc
     5581 ttcgccaatc cgaaactgtt taacgaaatg gtgaaaaacg aagacggcag cgtgcgccaa
     5641 ggctcgttcg cccgcgtcga acccgaaggc gaaaccatgc gcatgtggga agccatcgaa
     5701 acctatatga accgcaaaca gccgctcatc atcattgccg gtgcggacta tggtcaaggc
     5761 tcaagccgcg actgggctgc aaaaggcgta cgcctcgccg gcgtagaagc gattgttgcc
     5821 gaaggcttcg agcgtatcca ccgcaccaac cttatcggca tgggcgtgtt gccgctgcag
     5881 ttcaaaccgg gtaccaaccg ccataccctg caactggacg gtacggaaac ctacgacgtt
     5941 atcggcgaac gtacaccgcg ctgcgacctg accctcgtga ttcaccgtaa aaacggagaa
     6001 accgtcgaag ttccggttac ctgccgcctc gatactgcag aagaagtatt ggtatatgaa
     6061 gccggcggcg tgttgcaacg gtttgcacag gattttttgg aagggaacgc ggcttagagg
     6121 tcgtctgaaa agcaagacgt agcgtgggtc gggttcaaca ttttgctcat tcacgtaatt
     6181 ctcgatatgg caggcatcta ctgtaaatcg tcattcccgc gcaggcggga atccagaaag
     6241 tggaattgag gaaacctt
//
LOCUS       DAWHRA010000119         6258 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWHRA010000119 DAWHRA010000000
VERSION     DAWHRA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.119"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(391..825)
                     /locus_tag="WIB30_001698"
     CDS             complement(391..825)
                     /locus_tag="WIB30_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ3797732.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WIB30_001699"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WIB30_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ3797733.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WIB30_001700"
     CDS             complement(1556..2398)
                     /locus_tag="WIB30_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ3797734.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WIB30_001701"
     CDS             2653..3291
                     /locus_tag="WIB30_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ3797735.1"
                     /translation="MFQNFDLGVFLLAVLPVLLSITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WIB30_001702"
     CDS             complement(3743..4543)
                     /locus_tag="WIB30_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ3797736.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMVVFVIVLFGARNILKLAK"
     gene            complement(4679..5182)
                     /locus_tag="WIB30_001703"
     CDS             complement(4679..5182)
                     /locus_tag="WIB30_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797737.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5519..6160)
                     /locus_tag="WIB30_001704"
     CDS             complement(5519..6160)
                     /locus_tag="WIB30_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ3797738.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
BASE COUNT         1665 a         1570 c         1303 g         1720 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggtgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacagcccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccaatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 cgtcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttaccgct gccttctccg cgccccgttc cgtctccttt ggtaccagtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgca gcaggcgcgc
     2221 ctgcaccttc gggggcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctct ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttca cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatcta gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg cggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctactg ctgatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaggtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt gaccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaacc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gcttactgga taacaaagtc
     4741 caaaccaata gcaggcaaaa taaggcatcc accccccttc ttcattaagg atatatattg
     4801 agaaacaaat cgcaactaaa cagaaaaaac ttgggagata aagccatttc attcccctat
     4861 tcaagaatct agccaagata ggtattttgt attctacaaa aaagaaaggc atttccaagg
     4921 gaaacatgtc agataaaaac ttttgtttat tttttactat agatagaacc ttgcttctca
     4981 agagaaagcc attaataata ccgatgacag ctattaatat atagagaata gtataagtat
     5041 gaataatctt cattagacaa aaagaagaaa tggcagataa attacatacg atatattgga
     5101 atataaaata tttacggtct aaaccttgtt cagttgcaat ttttttaaaa ttgccttgca
     5161 taaaaaaatc aaaggcgtcc attaaactat ctttcacatt agaaatttaa aagctaaata
     5221 atacgacaaa caatgtgaag tactattcat ggtttattta aaaaataata ctattctgaa
     5281 cattatttag atacagaaat taacaaatta gaactaaaca agcttttaaa tactttaatt
     5341 ttattggaaa gctataaaag gaactataac tttacacact agtcacttct ttttaagagg
     5401 caaaagggat tgggaaggtc gtcttggaga taagcactgg tatttcggcc aatggtaaat
     5461 agagtttacc tcaaataggg tagaacctcc ttcatctgtc agttaataac agccactttt
     5521 acaatgccct gtcaaaataa agcggcacgc ccgatttttc actcatcgtc atcaaataac
     5581 ccatcacctt ttggggccat tcgatgccgc gcaccacggt cagattcctc aaaacgggga
     5641 aaaccaaaat atcctccata ccgattccgc cgttgatgcc gtctgaagca ccgtccatca
     5701 aattttccaa ctcttgcaaa tctgcgttta tccgttcgag gtattgggcg gttttattca
     5761 aattggcgga aaagctgccg atgcttttct cttttttgtc tgtaaaatat ttcaccgctt
     5821 ccggcgttgc aaattcaggc agcccgattt tgatcacgcg cggctgcacc agtttgtcgt
     5881 tgtatccgcc caccttgtcc agccacgccc gtatctcggg gcggacttcg tctttcagac
     5941 ggtcttcgcg gtcgaaatgc cgcacaatgt ccaaactctc gcccataaac gaaccgtctt
     6001 ctttttgcag gacgggcact tgtttcgcac cgatcatacc gatcggcgtt gcctcgtcgt
     6061 cgtttgccag cacggcttct tcaacgtccg cgccaaacag cccggcagcc atccgcgcac
     6121 gcacgcaaaa cgggcaatgg tcgtaaatat acagtttcat caaaatattc ctcgtcaacc
     6181 tgtcggtacc ggctacctta acacccggcg ccgcccgaaa caagtttatc ttcccgccta
     6241 tgcaccgtaa ataaataa
//
LOCUS       DAWHRA010000120         6148 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWHRA010000120 DAWHRA010000000
VERSION     DAWHRA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.120"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            142..576
                     /locus_tag="WIB30_001705"
     CDS             142..576
                     /locus_tag="WIB30_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ3797739.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(624..1967)
                     /locus_tag="WIB30_001706"
     CDS             complement(624..1967)
                     /locus_tag="WIB30_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ3797740.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1972..2625)
                     /locus_tag="WIB30_001707"
     CDS             complement(1972..2625)
                     /locus_tag="WIB30_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ3797741.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2776..3066
                     /gene="gatC"
                     /locus_tag="WIB30_001708"
     CDS             2776..3066
                     /gene="gatC"
                     /locus_tag="WIB30_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ3797742.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3129..4574
                     /gene="gatA"
                     /locus_tag="WIB30_001709"
     CDS             3129..4574
                     /gene="gatA"
                     /locus_tag="WIB30_001709"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ3797743.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4571..5503
                     /locus_tag="WIB30_001710"
     CDS             4571..5503
                     /locus_tag="WIB30_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ3797744.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDSLKTEARGISDGIQTEKPPRRTA"
     gene            5546..>6148
                     /gene="gatB"
                     /locus_tag="WIB30_001711"
     CDS             5546..>6148
                     /gene="gatB"
                     /locus_tag="WIB30_001711"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEZ3797745.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRP"
BASE COUNT         1559 a         1881 c         1519 g         1189 t
ORIGIN      
        1 ttttgggaat ctgttttacc ccaatatata aagcaccata ttaaggcgga gtgtcttccc
       61 cactttgacc cgaacccgga aaagacaccg cccaagccaa tcctgatgct gccccgacag
      121 ccaaccatta aggaaatcct aatgaacttt gctttatccg tcattatgtt gaccctcgcc
      181 tctttcctgc ccgtcccgcc tgccggagcc gccgtcttta cttggaagga cggcggcggc
      241 aacagctatt cggatgtacc gaaacagctt catcccgacc aaagccaaat cttaaacctg
      301 cggacgcgcc aaaccaaacc ggcggtcaaa cccgcccaag ccgacgcagg gaagcgcaca
      361 gacggcgcgg cacaggaaaa caatcccgac actgccgaga aaaaccggca gcttgaggaa
      421 gaaaaaaaaa gaattgccga aaccgaacgg cagaacaaag aagaaaactg ccggatttca
      481 aaaatgaacc tgaaggcggt gggaaattca aatgcaaaaa acaaggatga tttgattcgg
      541 aaatacaata acgccgtaaa caaatactgc cgttaatcgg ctctagcgca aacccgatgc
      601 cgtctgaagc ggcacggggt ttgtcatttc tgccagtagg ttttgacgtt gacgaactcg
      661 tacagcccga attcggacaa ttcgcgcccg taaccggaat ctttgactcc gccgaaaggc
      721 aggcgcaaat cgctgctggt atggcggttg ataaacaccg atcccgcctg tattttttcg
      781 gcaaaccgcc aagcgcgttc ggtatcggcg gtataaatgc aggcaccgag cccgaacggg
      841 gaatcattgg caagggcgat ggcatgttct tcgttttcgg cgcgcaaaat cagggcggcc
      901 ggcccgaata cttcttctct ccagacgcgg caggcaggat ttaccctgtc taaaaccgtc
      961 gcgggataaa accagcctcg cccttgtggg atttttccgc cggtcaggca taccgcgccg
     1021 tttgaaacgg catcttcaac ctgcccgtga accctgtccc gcaaatcttc gcggtgcagc
     1081 ggtgcaagcg tagtatcggg atgtttgggg tcgcccattt tcaatttagc gcattcggca
     1141 agaaacagcg tgataaaacg atcggctgcg gcttcggtta cgatgatgcg cttggcggcg
     1201 ttacacgatt gccccgcatc gcggaaacgg gaataacagg cttctgcggc ggcacgctcc
     1261 aaatcagcat cgggcatcac gataaaggcg ttgctaccgc cgagctccaa cacggttttc
     1321 ttaaggtttg cgcccgcgtg tgccgcaagg atgcgccccg tatgcgttga accggtaaac
     1381 gccattgcat cggtatcttc aaccgccttg agcgtgcccg cctcatccag ccacacgcct
     1441 gccagaggaa tgccgtctga agccaaatcg aacagtgcct gactgacgcg tgccacgctg
     1501 ggcgcgggtt tgacggcgca cgcgttgccc gcgcacatag cgggaacggc gaaacgcaat
     1561 acctgccaga cgggatagtt ccaaggcatg acggcaaaca ccacgcccaa aggctcgaag
     1621 cgcacctgac tcaaactcgc ctgcgtcgcg atggttttgt gggcaagcag ttcgggggca
     1681 aggcgggcgt aatagcgtat cagttcgata gacttgccga tttccgcacg gcattcgtgc
     1741 aagcagcgtc cgacttcctc acacaccatt tccgcaaaac gctctttctc cgcctccaaa
     1801 cggtcggcaa atttttgcag gcgcgcggca cgttcggtta cgcccagttg cgcgaacgcc
     1861 ccgccgcgca ttttcaaatc cgccagccgc cgttcaaact ccgcataatc ttgagcgggg
     1921 cggcggtaaa gcgtttcgcc cgtaaataca ttgacactgt gaaacatcga atcaacctgc
     1981 cagttgcggg aatatcgttt tcagtcccga cacaataatc tccaccgata ccgccgccag
     2041 catcataccc ataatgcggt ttaaaatcgt cagccccgtc gcgcccagca ggcggctgac
     2101 cttcccggca acgattaaaa tggcataaca aatcgcactg accaccaaac cggccgcgat
     2161 aatcaacgcg atgtcgccgt atgttttagc cgccgaagcg taaataatca cggtcgaaat
     2221 accgcccggg ccgatggtga tcggtatggc gatgggcacg acggcaatcg ctccggcatt
     2281 gcgggcgggg cgcgcctgcc ccgtttccgg ctgcgcgccg agattctgct tggcgggatt
     2341 gtcgttgccg ttcatcatcg aaatggcgat cagcagcacc aaaatcccgc cgccgacctg
     2401 aaacgaaccg acgctgatgc ccaaaacctt cagcagcgta ccgccgatca gcgcaaatac
     2461 cgcaatcacg gcaaacacgg caacggcggc cgtccgcgcg accttcctgc gctccttcgt
     2521 gctgtgcccg ttggtcaggt caaggtaaag cgacaacgcg ctaaacggat taatcagcac
     2581 caaaaaagcc acaatcagct tgccgatttc catgcccaat cccattattt ccccctcctt
     2641 caaacccgtg cggcaggcat ccgatgctgc aaattgccgc cgcaacggat ttttccgtta
     2701 taattaaaaa ttcaagcaat acgccccatc atacccgaac gacggtatct ttaccatcag
     2761 acaaggatgc ttttcatggc actgacactt gccgacgtag acaaaatcgc ccgactctcc
     2821 cgactgcacc tgactgcgga agaaaaagaa aaatcgcttc aagaattaaa cgacattttc
     2881 actatggtcg aacagatgca aaccattaac acagacggca tcgaaccgat ggcgcacccg
     2941 cacgaggccg ccctgcgcct gcgcgaagac gaagtaaccg aaaccgaccg cgccgccgaa
     3001 tatcaggcgg gtgctccgga agtacgcaac cgtctgtaca tcgtaccgca agttatcgaa
     3061 gaataatccg aatatgcttc agacggcatc agcaataccg cccgaagccc tttaaggatg
     3121 gaagatttat gacccaatac acattgaaac aggcaagcgt cctgttgcag tccaaacaga
     3181 tttccgccgt cgaactggca agcgcatacc ttgccgccat cgccgaaaaa aatcccgccc
     3241 tcaacggcta tatcaccatc gaccaagata aaacccttgc agaagcccgt gccgccgacg
     3301 aacgtatcgc gcagggcaac gcctccgcgc ttaccggcgt acccgtcgcc tacaaggata
     3361 ttttctgcca aaccggctgg cgcagcgcgt gcgcttccaa aatgctcgac aacttcatct
     3421 ccccctacac cgccaccgtc gtccaaaacc tgctcgacga aggtatggta acgctcggcc
     3481 gcaccaatat ggatgagttc gctatgggtt cgaccaatga aaactcattc tacggtgcag
     3541 ccaaaaaccc atggaatctt gagcacgtcc ccggcggttc gtcaggcggt tccgccgccg
     3601 tcgttgccgc gcgcctcgcc cctgccgcgc tcggttcgga caccggcggc tctatccgcc
     3661 aacccgcatc gcactgcggc attaccggca tcaaacccac atacggcacg gtttcccgct
     3721 tcggtatggt cgcctacgcc tccagcttcg atcaaaccgg cccgatggcg caaactgccg
     3781 aagactgcgc gattctgtta aacgcgatgg caggtttcga ccccaaagac tccaccagcc
     3841 tcgagcgcga aaaagaagac tacacccgcg atttgaacca accgctcaaa ggtttgaaaa
     3901 tcggcctgcc caaagaatat ttcggcgaag gcaacagcgc cgatgttctg acggcattgc
     3961 aaaacaccat tgatttgctg aaagcccaag gcgcggaatt gattgaagtt tccctgccgc
     4021 aaaccaagct gtccatcccc gcctactacg tcctcgcctc cgcagaagcc agcaccaacc
     4081 tttcacgtta cgacggcgta cgttacggac accgtgccgc ccaattcgcc gatttggaag
     4141 aaatgtacgg caaaacccgc gccgaaggtt tcggcagcga agtcaaacgc cgcatcatga
     4201 tcggcactta tgtactgtcg cacggctact acgatgccta ctatctcaaa gcccaaaaac
     4261 tgcgccgcct cgttgccgat gattttcaga cggcatttgc acggtgcgac ctcatcctcg
     4321 cgccgaccgc acccactgca gccccaaaaa tcggagcgga tgcttcgccg gttgaaacct
     4381 acttgagcga tatctacacc atcgccgtca acctcgccgg actgcccgca ttgaccctgc
     4441 ccgcaggctt cagcggcggc ggactgcccg tcggcgttca gcttgtcggc aactacttcg
     4501 ccgaagccaa aatcctcggt gcggcgcatc aaatccaact caacagcgat tggcacggca
     4561 aacgacccga atgaagcaga accgcacctt taccttcccc gattttcgca ccgtttacag
     4621 ctatgcgcct ttatatcggc tgcaacattt aaaatacaca ttgcgaaaat ttttcggaaa
     4681 aaaagaaatt tacgccttcg agcagtttgt caacgcctcc cctatccgtc aggggctgtt
     4741 cctccactgc ccgcaaaatg cctatccgct gctgcgcgaa tttgttgaca ggcgttttaa
     4801 ctgcaaacgc cgtttagatg cgatgacggc agattttctc atggcggaaa aactgttcgg
     4861 cacagacatc ctgcaccaaa tggaagacta ccgcttccat ttggtcttgg cgcacctttc
     4921 agacggcatc agcttgtggc tcaaccgcaa cgacaactgc gtcgaagaag gcgcgtggtc
     4981 tttatctttg cgcgacgaag caggcaaccg gctgtatatg gcgactttcg cctttgtcgg
     5041 cacacacctg ctgacagcct ccgtacaagg gccggcgggt gaagaagcca aagacaccgt
     5101 ccgccgcata accaaacaac tccacggctt gcgtccccaa caactgatgg taaccgccct
     5161 gcaatatttc gccgccgtac tcggcttgga cggcgcaatg ggcattgcac aaaaacatca
     5221 ggtcaaactg cgctggaaac ttaaaaagcg cgtcaaaatg aattacgacg cattctggca
     5281 ggaatacggc gcaagtttgg aacgggacgg ctactggcat ctcccccaaa cccccgcccg
     5341 caaagacctt gccgacatcg aaagcaaaaa gcgttcgatg taccgcaagc gttatgaaat
     5401 gctggacaat atggttgcag agatgaaaga cagtctgaaa acagaagcac gcggcatttc
     5461 agacggcatc caaacggaaa aaccgccccg ccggacagcc tgacgcgaag actatcgaat
     5521 tgatatttta gagaaagaag ctcttatgac ctgggaaacc gtaatcggct tggaaatcca
     5581 cgtccaattg aacaccaaat ccaaaatctt cagcggcgca tcgaccgcat tcggcgcaga
     5641 acccaacgcg cacgccagcg tagtggaatg cgcgctgccg ggcgttttgc ctgtgatgaa
     5701 ccgtgaagtc gttgaaaaag ccatcaaatt gggtttggct ttagatgcga aaatcaatca
     5761 gaaaaacgtg ttcgaccgca aaaactactt ctatcccgac ttaccaaaag gttatcaaat
     5821 cagccagttg gacttaccga ttgtcgaaca cggcaaattg gaaatcgtag tcggcgacga
     5881 tgtgaaaacc atcaacgtaa cccgtgcgca catggaagaa gacgcaggca agtccgtgca
     5941 tgaaggcttg aacggcgcaa ccggtatcga cctgaaccgc gccggcacgc cgctgttgga
     6001 agtggtatcc gaacctgaaa tgcgttccgc cgccgaagcc gttgcctacg ccaaggcctt
     6061 gcacagcttg gtaacctggc tggacatttg cgacggcaat atggcggaag gctcgttccg
     6121 cgtcgatgcc aacgtatccg tgcgcccg
//
LOCUS       DAWHRA010000121         6129 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWHRA010000121 DAWHRA010000000
VERSION     DAWHRA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.121"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            199..645
                     /gene="yciA"
                     /locus_tag="WIB30_001712"
     CDS             199..645
                     /gene="yciA"
                     /locus_tag="WIB30_001712"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ3797746.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVDVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
     gene            complement(696..1517)
                     /locus_tag="WIB30_001713"
     CDS             complement(696..1517)
                     /locus_tag="WIB30_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ3797747.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYQRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1636..2094
                     /locus_tag="WIB30_001714"
     CDS             1636..2094
                     /locus_tag="WIB30_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ3797748.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2258..3373)
                     /gene="lst"
                     /locus_tag="WIB30_001715"
     CDS             complement(2258..3373)
                     /gene="lst"
                     /locus_tag="WIB30_001715"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ3797749.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAEW
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3474..3578
                     /locus_tag="WIB30_001716"
                     /pseudo
     CDS             3474..3578
                     /locus_tag="WIB30_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            3806..6031
                     /locus_tag="WIB30_001717"
     CDS             3806..6031
                     /locus_tag="WIB30_001717"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ3797750.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFTRHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1640 a         1801 c         1359 g         1329 t
ORIGIN      
        1 ttcagttgct tagggtagga tttggcggga ttggcggact gaagcccact ctacagcccc
       61 gccctacaat acgccttgcg aatctgttcc gcgcccgccc ttgtatgtct caaatgggtt
      121 aaactacaca cttcccgact tcccgtttcg ggcaggggtg caggttcgat ttatatttca
      181 ataaaacaag gaaactttat gcagcacgaa gaaggcaacc gccaacgccc tcaaggcgaa
      241 ctgctcctgc gtaccgtcgc tatgccgcgc gataccaatc ccaaccaaga cattttcggc
      301 ggctggatta tgtcgcagat ggatttgggc ggcggcatat tggcggcgga aatcgcgcgg
      361 ggacgcatcg ttaccgtcgc cgttcaggaa atgaacttca tccgcccggt caaggtcggc
      421 aacgtcgtct gctgctacgg gcattgcgtc cgcgtgggca acacttccct ccagcttaaa
      481 gtcgacgtct gggtgaaaac tttgatgaac gattgcgtta ccgaagaccg ctacctcgta
      541 accgaagccg tgttcactta tgttgccatc gatgcggaag gcaatccgcg cccgattccg
      601 aaagaaggca accccaaatt ggcgggctta ttgcctactc cgtaaaaata cccgtaaaaa
      661 tgccgtctga aactgtcttc agacggcatt ttgcatcatt tgaaccagga cctgatgcgg
      721 gaaaacagcg acatactctg tttttcaaag tcttcccgca cttcgggcac ggcttttttt
      781 atcactttca ttcctgcaaa agtctgcgcc acgggcataa tttcaatcgt gttcacattc
      841 atatgcgccg gccgctggta cagccacaat gcggtttccg caatatcttc ggggcggata
      901 aattccacac cctcatacac gcccgccgcc ctctcgtcat cgcctttgaa gcgcacattg
      961 gaaaactccg tattgccgca caaacccggc tcgatattgg taacgcggat gttcttatcc
     1021 gccaactccg cgcgcaaatt caggctgaac tggcgcacaa acgccttggt cgccccgtaa
     1081 acgttgctgc cggcataagc ataattgcct gcaatcgaac ccaaattcat cacataaccg
     1141 ccgccgcgtt ccaccatttg cggcaaaatt ttgcgcgtca ggaacgtcaa acccaaaaca
     1201 ttggtttgaa tcatcgtttc ccaatcttca aaatccgcct tgtccgccgt gtccagaccc
     1261 aaagccagcc cggcattgtt gatgaggcag tcgatgtcgg aaaattcatc ggggatgccg
     1321 tttaaggcgt tttccaccga ctcgcgtcgc gacacgtcca tttccaaagg gtaaaacaaa
     1381 gcacccaatt catccgccaa ggcctgaagc ctgtccgcac ggcgcgccgc accgataacg
     1441 cggtatcccg ccccgacaaa cgcacggcac atcgcttcgc cgaatcctgc cgaagcaccg
     1501 gtaattaaaa cagccattgt ttttcctttc tttctcgttt cccaaacctg tttcaggtat
     1561 cataacaccc ttcagacggc atcgcgccga ctgaaaatga caacaaccat accaccacga
     1621 caggacacat tcaatatgaa aacccaaatc agccttgctg ctgccgccat cacactgctc
     1681 ctttccgcct gcggaggcag cggaatacct tcccaaccca aaggcgaaat ttccgaaaac
     1741 cgcaccgccg cgttcaaatc catgatgccc gaattcacac gcatgggcaa aatggtcaaa
     1801 gacgaagaac cttacgatgt cgaaaaattc aaacaggcgg cagcgtcgtt tgccgaaagc
     1861 agcaagaaac cgttcacact ttttgagtcc gatccgcaag gcaacggccg cgccctgccc
     1921 gccgtttggt cggatggtgc aaaatttgaa gccgaaaaaa caaaattcgc cgccgccgtc
     1981 gaaaaactca acgccgcagc acaaaccggc aaactggatg aaatcaaagc cgcctacggc
     2041 gaaaccggcg caagctgcaa atcctgccac gacagtttcc gcgcacccga ataacatccg
     2101 aaacagaaca atgccgtctg aagccttcag acggcatttt ctttgccatt tcaccaagcg
     2161 caaagcaatc aaaaattatc ttcaattcaa aaaatacgcc atatcctgat ttttgcctta
     2221 cttactccgc cattttctat tttgatttgc tatgcgatta atctttatcg tcaaatgtca
     2281 aaatcgggat gccggattgg gtaaacaggg catacaccgg cttgagccaa taatcttccg
     2341 gaagggaagc cggtttcaat gcgtaaacgt gcacattggg aaaatccttc atcgtcaacg
     2401 ccgcaccgct gaaaaaggta taaatttcat acctcgtatg cgggtttttc ttaatttcgc
     2461 gcaaaatata gtcttcgatg acatagggcg aatttaatgt ggttacgccg gaaagcccgt
     2521 aggtttggcg ggggtgcggt gcgacatatt gtatgttgaa gttttttgcc gccttttccg
     2581 aaatttcctt catctccttg tcaggcgaac ccaaaagtat ccgcaccgtg ccgcccgttt
     2641 cgtcccccgc cttcagttcg gacgcatcga acagcggcag gtaagtcatc ttgcggcgac
     2701 cgtcgtccat aatgtttttc aaacccttga atatcgtgta atgctcgtcg gaagcattac
     2761 gggttttggc gatgctccaa tctccgatca tcatccgggc aaaattccgc ttgatcgtcc
     2821 cgtttacaga aaactcatca cccaaatagc tgctgctttg aattaaattg attgtcccgt
     2881 cgtcaaaggt tttgatttcc gcatccgggt aagtgctcaa aaaggcggca atgctgactt
     2941 tttccaaact tgccaaataa atccgcttga ccttcggcag cagcattgcc ttcaccttca
     3001 gctccgccat cgtcggaatg aaattaaacg atttgttcaa accgtagggc aggtggaaaa
     3061 aatacgccca ctccgcctta tcctttatct gattgaaata ataatcgtat ttttcattcc
     3121 tgttttcaga catcagcacc acataaaacc gctcccccgg atgctgcgcc ataatccttt
     3181 ctgccacctt catctgcaat atggtatagc agaaaatcag attgaccggt tccccctctt
     3241 cattgaagag tttgtccttc agcagggaaa ccgcattcct ttccccctga tttacccggt
     3301 caaatgtata aaatatcccg aaacaaaaaa caatcaggca caacacggtc aaacaagcct
     3361 ttttcaagcc catatcccta aaactccatt ccgacaaatt gaacatacgc cccgagtgtc
     3421 ccgggacgga aaaatgcgga attttagcag atattccgac agttaccgaa tattatagtg
     3481 gtttaaattt aaatcaggac aaggcgacga agccgcagac agtacaagca gtacgacaag
     3541 gcgaggcaac gctgtactgg tttaaattta aaccactata aaatcaattt caccgaaaat
     3601 atgccgccgc acatcaggca cagcacctcc gaccgccacc aaacgcggga aaaccatttc
     3661 ccccttcctt tcacgctgcc gcgttcaaac acgccaagat cgaacaaagc gcgaacaaag
     3721 ccgccaaaat atgccataat agccgcgcac attccccgcg caatgtgttc caaaagaaaa
     3781 aaccaaccta caagagaata atcctatgac tcaaaaatcc accattgttt atacccatac
     3841 cgacgaagcc cccgcgctgg cgacccaatc gctgctgccg attgtgcagg cttttacccg
     3901 ccacgccgat attgatgtca aaactgccga catttccctg tccggccgta ttttggcggc
     3961 attccccgaa tatctgaccg aagcgcaacg cgtacccgac gcgcttgccg aattgggcga
     4021 actggtaaaa caacccgatg caaacgtaat caaactgccg aacatcagcg catccgtacc
     4081 gcaactgacc gccgcgatta aagaattgca gtctaaaggc tttgccgttc ccgattatcc
     4141 tgccgacccg caaaccgatg aagaaaaagc cgtgcgcgaa cgctacgacc gcatcaaagg
     4201 cagcgcggta aaccctgtcc tgcgtgaagg caactccgac cgtcgcgccc ccaaagcggt
     4261 gaaaaacttt gccaaaaaaa atccgcacag catgggcgca tggacaaaag actccaaaac
     4321 ccacgttgcc accatgcaaa gcggcgactt tttccataac gaacaatccg ttaccgtacc
     4381 tgaagcgact tccgtatcca tcgtgttcac cgacaaacaa ggcaataaaa aagagctgcg
     4441 cgcgcctgtt gccctgaaag ccggcgaaat catcgacgcg accgtgatga gtaaaaaagc
     4501 cctgctcgcc ttccttgccg aacaagtaaa agatgcgaaa gcaaaaggcg tgttgttctc
     4561 gctgcacatg aaagccacta tgatgaaagt gtccgacccg attatcttcg gacacgccgt
     4621 caaagtattc ttcgcgcctg tttttgaaaa attcggcgac aaactggctg ctgccggcgt
     4681 caacgttaac aacggcttcg gcaacctgct tgccaatctg gacaaactgg atgcggacac
     4741 ccgcaccgcc gtcgaagccg aaatcgccgc cgtttacgcc gccaaccccg atttggcgat
     4801 ggtcgattcc gataaaggca ttaccaacct gcacgttccc agcgatgtca tcgttgatgc
     4861 ttctatgcct gcgatgattc gtaactccgg ccgtatgtgg gataaagacg gcaaagcgca
     4921 agacaccaaa gccgtgattc ccgacagcag ctacgccggc gtttaccaag caaccatcga
     4981 cttctgccgc gaacacggcg catttgaccc gacaaccatg ggtactgtgc ccaacgtcgg
     5041 actgatggcg caagcggcgg aagaatacgg ctcgcacaac aaaacctttg aaatcgaagc
     5101 cgacggccaa gtccaagtca ttgatgcggc aggaaaagtc ctaatgcagc acgacgttga
     5161 agccggcgac atctggcgta tgtgccaaac caaagacgct ccggttaaag actgggtaca
     5221 acttgccgtc aaccgcgccc gtctgagcaa cacgcctgcc gtgttctggc tcgacgaaaa
     5281 ccgtccgcac gacaagagcc tgctcgccaa ggttaaagcc taccttgccg aactggatac
     5341 caatggcctc gacatccgcg tcctcgctcc tgaagaagcc gccaagttca gcttgggtcg
     5401 tctgaaaaac ggcgaagaca ccatctccgt aaccggtaat gtcttgcgcg actacctgac
     5461 cgacttgttc ccaattttgg aactcggcac aagcgcgaaa atgctgtcta tcgttccatt
     5521 gatgaacggc ggcggtatgt ttgaaaccgg cgcgggcggt tctgcaccga aacatgttca
     5581 acaattcctc gaagaaaacc atttgcgctg ggactcgctg ggcgaattcc tcgcactcgc
     5641 cgtatcgttt gaacatttgg cgcaaaaaac cggcaatgcc aaagcccaag tcctcgccga
     5701 cactttggat gcagccaccg aaaaactgct gttgaacgac aaatcgccta aacgtaaagc
     5761 cggcgaactc gacaaccgcg gcagccattt ctacctcacc ctctactggg cgcaagaatt
     5821 ggcggcgcaa gacaaagatg ccgaactgaa agccgcattt acgccattgg cagccgcttt
     5881 gaccgccgac gaagcgaaaa tcgttgccga gctttccgcc gtacaaggca aagcggccga
     5941 catcggcggc tactacgccg ccaatcctga aaaagccgca caagtgatgc gtccgagcgt
     6001 aacctttaat caggcactaa acgccttata agcacaaagg taaaaatgcc gtctgaacat
     6061 tcgtcgttca gacggcatta gttatcccta tctgcctgat tatgattaaa cgccgcaccc
     6121 attggatat
//
LOCUS       DAWHRA010000122         6091 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWHRA010000122 DAWHRA010000000
VERSION     DAWHRA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.122"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(140..1087)
                     /locus_tag="WIB30_001718"
     CDS             complement(140..1087)
                     /locus_tag="WIB30_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ3797751.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(1102..1509)
                     /locus_tag="WIB30_001719"
     CDS             complement(1102..1509)
                     /locus_tag="WIB30_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ3797752.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIITHP"
     gene            1590..2249
                     /gene="ung"
                     /locus_tag="WIB30_001720"
     CDS             1590..2249
                     /gene="ung"
                     /locus_tag="WIB30_001720"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ3797753.1"
                     /translation="MDTWHDALGNEKHQPYFQKILNAVRQERLSGQIIYPPETDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDINGFSI
                     PDHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLAAHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            2352..2999
                     /locus_tag="WIB30_001721"
     CDS             2352..2999
                     /locus_tag="WIB30_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ3797754.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            3072..3545
                     /locus_tag="WIB30_001722"
     CDS             3072..3545
                     /locus_tag="WIB30_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ3797755.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTFTNLVTAVWCI"
     gene            3533..3847
                     /locus_tag="WIB30_001723"
     CDS             3533..3847
                     /locus_tag="WIB30_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEZ3797756.1"
                     /translation="MVHLSEIAAQDYNLNLPRYIDSGEVEDLQNLAAYLYGGIPAHDM
                     DALEAYWQVLGRMKNELFAEHDGRFTTIQRNRLQIFPTLNSLKSCGIGVEFRANLVQP
                     QI"
     gene            3889..4833
                     /locus_tag="WIB30_001724"
     CDS             3889..4833
                     /locus_tag="WIB30_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ3797757.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEDANLTV
                     VFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            4905..5108
                     /locus_tag="WIB30_001725"
     CDS             4905..5108
                     /locus_tag="WIB30_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ3797758.1"
                     /translation="MPTKTFRGSRFGCPHRLIRRVHELTHLLERHRNARFMAHMDNFL
                     PNWQSIKQQLNALELFAQIYNLT"
     gene            complement(5398..5913)
                     /locus_tag="WIB30_001726"
     CDS             complement(5398..5913)
                     /locus_tag="WIB30_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797759.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
BASE COUNT         1564 a         1508 c         1513 g         1506 t
ORIGIN      
        1 gacagtacaa atagggcaac gccgtactgg tttttgttaa tccactatat ttgaagcgga
       61 atacaacctt gcccgggttt aaataaaaat gccgtctgaa ccccgaaaac tggacttcag
      121 acggcatttt catcacggct tatttggcgg ttttgctgct gtcgataatt ttcataccgg
      181 cagaaatcag gctgccgatg tcggcaacat tggcgggcat aatcagcgta ttgctttctt
      241 tggcaagatt gttgaacgca gcgacgtatt gttccgcaat cttcagattg accgcatccg
      301 caccgccttg ggtttgaagg gcggcggcaa tttgacggat ggcttcggca ttggcttcgg
      361 caacaaggcg caaggattcc gcttcacctt tggcgcggtt gatgcgggcg attttctcgg
      421 catttgacgc attgaccgca gcctgagcct cgccttcgga ttgttggatt tcggcttcgc
      481 gctgaccact ggcaaggttg atttgttcga ttttacgacc ttcggattcg gcgatacggg
      541 cgcgtttttc gcgttcggca gtaatttgcg cctgcattga gcgaaggatt tcttgcggcg
      601 gaaccaagtc tttaatctca taacgcaaaa ccttcacacc ccaagccccg gccgcctcgt
      661 ccaaagccgc aacaacagta ctgttgattt cgtcgcgttc ttcaaacgtt ttgtccaact
      721 ccatacgccc gataacggaa cgcagcgtcg tttgggcaag ctgggtaatc gccataatgt
      781 agttgctcga accgtatgag gcgagtttgg ggtcggttac ttggaaatag atgatgccgt
      841 caacagtcag ctgcgtattg tcgcgcgtga tgcagacctg gctgggtacg tctaaaggga
      901 tttctttcag cgaatggcgg taggcgacgc ggtcgataaa gggaatcaaa atattcaaac
      961 cggccgtcag ggcgcgatgg aaacgcccca gcctttcgac aacgtggact tcctgttgtg
     1021 ggatgacaac aaaggatttg aaaccgaaaa cggcgacggc taccaacaag ataatgaaaa
     1081 attccataat tcctccgagt gttaagggtg tgtgataata agaaggttgc cttccttgcg
     1141 gacaatgagg gcgcgagttc ctggttcaag ctcttcttgc cccgtatttt gagcctgcca
     1201 gtgcgtaccg cgataaaaaa cttcgtaacg gttgccgcct gtgtgtcgga ggatttcgac
     1261 atattgtccg gcatccaaat cctgatatga atccgtttca acttttctaa cggcggtttt
     1321 ggcgtgtacg aaccaaatac ccagcgcgga aagcagagcg gcggtcaaga cggcggcagg
     1381 cgtactgccg gtcagcccgt aagcaatgcc cgaacccgcc aaagccgcgc tgacaaccaa
     1441 aagataaacc gttcccgtca ataattcgat gattaagacg gcaacagcgg caacaaacca
     1501 tacagtcata catttcccca caaagtgcgt cgtttgacaa aatcacgcga tatcagcagt
     1561 atagccgaat ttgaaaggat agggcagata tggatacttg gcacgatgca ctgggcaacg
     1621 aaaaacatca accgtatttt cagaaaattt taaatgcggt caggcaggaa cgtttgtcgg
     1681 gacaaatcat ctatccgccg gagactgatg tgttcaacgc attccgcctg acagcgttcg
     1741 accgggtcaa agtcgttatt ctcggacaag atccgtatca cggggcaggg caggcgcacg
     1801 gtttggcatt ttccgtccgg cagggtgtcc gcataccgcc gtctttactc aatatctaca
     1861 aagaattgga aaccgacatc aacggttttt ccattcccga tcacggctgc ctgacggctt
     1921 gggcggagca gggcgtattg cttctgaaca cggttttgac ggtgcgtgca ggacaggcgc
     1981 attcgcacgc ccttttaggc tgggaacgct ttaccgatac cgtcatcagg cagcttgcgg
     2041 cgcaccgcaa gcaccttgtc ttcatgttgt ggggcgggta tgcgcaacaa aaaaggaaac
     2101 tgatagacag tcaaaatcat ttgatactga ccgcaccgca tccgtctcct ctgtcggcat
     2161 atcgcggttt tttcggctgc cgccattttt cacaggcaaa cagctatttg agccggcacg
     2221 gtatcgatcc gataaactgg aagctgtgaa tgccgatata gccgttgccg ccggcgtgtt
     2281 aaaatcgcgt ttgatttgta atttccattt attaggcaaa accttatggc aatcaaaaaa
     2341 aaatcagctt tatgcatcac tttgggcgag ctgcgacgag ttgcgcggcg gtatggatgc
     2401 gagcctttat aaagaactat gtgcttacgc tattgttttt aaaatatgtt tctgataagc
     2461 ataagtacgg cggcggcatg attgagctgc acgccggtac gacttttgac gacatcgtca
     2521 aactcaaaaa caccgccgac atcggcgacc gcctgaataa gattatcgcc caaattgccg
     2581 aagccaacga cttaaaaggc gtgatcgacg ttaccgactt caacgacgaa gacaaactgg
     2641 gtaaaggtaa ggagatgatc gaccgtttga gcaggcttgt cggcattttt aaaaagctca
     2701 acctttcttc caaccaagcc gaagacgacg atttgttagg tgatgcctac gaatacctga
     2761 tgcgccattt tgcgaccgag tcaggcaaat ccaaagggca gttttacacg cctgccgaag
     2821 tctcccgcat tatggcgaag attatcggaa tcagcgcaga ttgccgtcca gcaccagcgt
     2881 ttatgacccg acctgcggct cgggttcgct gttgctcaaa gccgccgccc aagccggcag
     2941 ccaaatcagc ctttacggtc aggaaaaaga tgtggcaacc gcgtcccttg cccgtatgaa
     3001 tatgattttg cacaacaacg aaaccgccga aatcaacacc gggaacacct tgtccgattc
     3061 gtctttccgt gatgaaaacg acgggcttaa gaccttcgat tttgccgttg ccaatccgcc
     3121 ttatcccgcc cgaaaaaaac ggcgattacg cctttttgct gcatctgctc aaaagcctga
     3181 aaccaagcgg caaaggtgcg attattcttc cgcacggtgt gctgtttcgc ggcaatgccg
     3241 aagcgcgtat tcgcacggaa ttgcttaacc ttgaccttat taaaggcatt atcgggctgc
     3301 ctgccaacct gttttacggc acgggcattc ctgcctgcat catcgtcatc gacaaagaac
     3361 acgcccaaac cgcccaattt gccgaagagg gaacaaacca agttatcagt ggcggcagcg
     3421 tgtttatgat tgacgcatcg cgcggcttca ttaaagacgg caacaaaaac cgtctgcgtg
     3481 agcaagacat tcacaaaatc atcgacactt tcacaaacct cgttacagcc gtatggtgca
     3541 tttaagcgaa atcgcagcac aagattacaa ccttaatctg cctcgctata tcgacagcgg
     3601 agaagttgaa gacctgcaaa atctcgccgc ctatctttat ggcggcatac ctgcgcacga
     3661 tatggacgca ttggaagcct attggcaagt tttaggccgt atgaaaaacg agttgtttgc
     3721 cgaacacgat ggccgcttta ccactataca acggaatcga ttgcaaatct ttcccactct
     3781 caacagctta aaatcctgcg ggattggtgt ggaatttagg gctaatctag tacagcccca
     3841 aatttaatcc actataaaat cgaaagcagc caaatcaaag cccatatatt ggcgcacccc
     3901 gattacgccg ccttcaaagc cggacacctg gcaaagtttg ccgcgtggca cactcaaaac
     3961 gaccttgccg ccatccaacc gggcaggctt atccggaaat ggagcgaaag cctgctggac
     4021 gcgttcaaac ccggcagcct gattgaagaa tacgatttct accaaatcct gacggactac
     4081 tgggcggaaa ccctgcaaga cgatgtttat ctcatcgccc aagatggctg gaaggcggtt
     4141 aaaaacctgg ccgaaatcac caaagaaagc gatgaagacg cgaacctgac cgtcgtcttt
     4201 gaggaaaccg aaaccggcaa aaaaggcaaa gccaaaacca agcgcatcag caaaaaatac
     4261 cgcagcgaag tcatcgcccc cgagctggtt gcccgccgct acttttcaga cggcatcgcc
     4321 aagctggaag aaaaacaaag cgagctggaa cgcctaagcc aagaattgga aaaccacata
     4381 gaagaacacg gcggcgaaga gggtgcgctg aacgacgtat tggatgcaaa aggcaaactt
     4441 tccgccaaac ttctgaaaac cgcattggaa gaaagcggca tagaagaagg cgaacgggct
     4501 gttttacaaa ccacccaaac actgatgacg caggaaaaag ccgcgaaaga cgcagtcaaa
     4561 acccaaatcg aagccctgaa ccttgccgta ttcaaacaat tcggccgact ttccgaagcc
     4621 gaaatcaagc agcttgccgt tcaagacaaa tggcttgccg atttacaaag ccgaatcgaa
     4681 aatcgcttgg aaaacagtat tcagcagctt atcagccgct tgaacacgct ggaagaccgc
     4741 taccgcagcc cgatggccga gcttgcccga gaagtggaaa agtggcaaag caaagtcaat
     4801 gcccaccttg aaaatatggg ttttggaggc tgaaatggcg gcacagacag gctataaggc
     4861 gagcgggttt tgagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat
     4921 ttaggggatc gcggttcggg tgtccgcacc gcttaatacg tcgtgtccac gaactaaccc
     4981 atttgctcga acgccatcgc aacgcccgtt ttatggcgca tatggacaac tttctcccaa
     5041 actggcaaag catcaaacaa cagcttaatg ccttggagtt atttgcacaa atatataatt
     5101 taacataata tacattatgc gaactatcgg aaacagttgt acgtgtccct gtggtctttc
     5161 caagtaggaa aattaaagta tggacaatgc ggctgaatgt atagcccgga gcatccgcgt
     5221 atccgaaggt actgaacgat atgccctgca tccctttcga cgcttccatc cagccggtat
     5281 tgggaaatac gttgaccttc aatacgatat acaggcagcc gacacacagg atatgccgct
     5341 gctttttcat catttcttct gtcaaatcct tggaacagtt gatttgaaaa cacggcttta
     5401 catacgtccc aattctcttt gcagtgcttg gatattttgc tgcctgtcca atacattgct
     5461 ttgcaatgcg ttgatttctt gatggttgat gttgccgcct tttgccagac gtgcttgtga
     5521 taacattttt tgggcttcaa ccaatgcttt gcgttcgttg ctcaattctg tttcgagaat
     5581 ggagcgtctg ctgttgtttg agggtgcttg ttgcggcggg ggcgtattgg attttgccgg
     5641 cttggatact gttttgaccg gggctttata tttgacagcc tgtccgccgt ttgacggtga
     5701 tggtgtcggt tcggatgttt ggggcgggat atagcgttcg ctgctgtagt tgccgattgg
     5761 gggcaaatcg gttgagaggc agcttttgga cggcttggtg gtgtaaacgg tttctccgtt
     5821 gattgtgcag gtgtagattt tggccgcatt cgcacccaat gggcttgaaa tcagggaaaa
     5881 gttgattagg attaagggga gttttgattt cataatgtgc ttgatgctcg gataattatt
     5941 tgattttttg gtattttttg taatgctttc ttggcgtttt acgggctttt cttttcggtt
     6001 ggttgctttt gattttcttt cggggctttg gcagcctgct tgttcttttt cccggcaggt
     6061 tttttgtttt tctttttact ttctatgtgt a
//
LOCUS       DAWHRA010000123         6044 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWHRA010000123 DAWHRA010000000
VERSION     DAWHRA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.123"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..220
                     /locus_tag="WIB30_001727"
     CDS             <1..220
                     /locus_tag="WIB30_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797760.1"
                     /translation="GISGIAPIFKIITDKQGRFVSGNIIPITQVGDKIPKIDPEKTVI
                     GRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            complement(298..1350)
                     /gene="bioB"
                     /locus_tag="WIB30_001728"
     CDS             complement(298..1350)
                     /gene="bioB"
                     /locus_tag="WIB30_001728"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ3797761.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKAFGIKVDY"
     gene            complement(1557..2933)
                     /gene="mpl"
                     /locus_tag="WIB30_001729"
     CDS             complement(1557..2933)
                     /gene="mpl"
                     /locus_tag="WIB30_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ3797762.1"
                     /translation="MKHIHIIGIGGTFMGGLAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIDVYEGFDAAQLDEFKADVYVIGNVAKRGMDVVEAILNLGLPYISGPQWLSENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHYL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWDVAEALAPLGGRLNVGKDFDAFVAEIVKNAEVGDHILVMSNGGFGGIH
                     GKLLEALR"
     gene            complement(2995..3300)
                     /locus_tag="WIB30_001730"
     CDS             complement(2995..3300)
                     /locus_tag="WIB30_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797763.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSAAGIKPH"
     gene            complement(3312..3656)
                     /locus_tag="WIB30_001731"
     CDS             complement(3312..3656)
                     /locus_tag="WIB30_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797764.1"
                     /translation="MRIFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLSCYY
                     DEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKELDEV
                     LLKYIPFFEEER"
     gene            complement(3846..3998)
                     /locus_tag="WIB30_001732"
     CDS             complement(3846..3998)
                     /locus_tag="WIB30_001732"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797765.1"
                     /translation="METHLIVQCRGGGGFGLGVVEMVLTDIAQNRANGIRTNKEFGKK
                     FVKSTN"
     gene            complement(3983..4123)
                     /locus_tag="WIB30_001733"
     CDS             complement(3983..4123)
                     /locus_tag="WIB30_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797766.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LI"
     gene            complement(4133..4918)
                     /locus_tag="WIB30_001734"
     CDS             complement(4133..4918)
                     /locus_tag="WIB30_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ3797767.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEAKMPSEEVRKLTAVLPEDGFFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVILGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLVRYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            complement(4992..>6044)
                     /gene="tilS"
                     /locus_tag="WIB30_001735"
     CDS             complement(4992..>6044)
                     /gene="tilS"
                     /locus_tag="WIB30_001735"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ3797768.1"
                     /translation="KVCVEKNGLGIEAAARQKRYAAFAEKGFDVLALAHHRDDQIETF
                     MLAVARGGGLRALAAMPAVRPFGEKGIIWRPLLPFSRQDIWDYAQKHGLPNIEDESNT
                     DTAYLRNRFRHRILPELSAQIPHFGRHVLNNVRALQEDLALLDEVVVQDCRWVCGAGY
                     FDTARWLTFSPRRKTHILRHFLKENGIPVPNQNALADIARVLTEAKTGRWNLQGFELH
                     HYAGRLFVFRLEKTDKLRFLKDRQISGNLREILTGQGFVLKRHPFGLPEHLLEQDGIL
                     RTVAASDTLAMGGIHKDVKKILQGKRVLPVLRPIWPLVADSGNRPLALANCCADFQYS
                     VSDGILPVHPDFPILF"
BASE COUNT         1371 a         1749 c         1478 g         1446 t
ORIGIN      
        1 cggcatcagc ggcatcgccc ccatttttaa aattatcacc gacaaacagg ggcggttcgt
       61 ttccggcaac attatcccca tcactcaagt cggcgataaa atccccaaaa tcgaccccga
      121 gaaaaccgtt atcgggcgga ttatttatct gaaccgcagc gatttcccca aggggaacgg
      181 gctggatgtc tcgcccgacg gcagcatcac gcgccggtaa aaagatattg gaaatgccgt
      241 ccggacaaat gccgtctgaa agcctttttc cgggtttcag acggcatttt tcaatcgtca
      301 ataatccact ttaatcccaa aggctttttc cacttcggcg acctcgcctt ccggttcaaa
      361 ctgcaaggga tacaggttga gcttttccat caggattctg tcgccgtcct catcaggatt
      421 gcccgtggtc agcagcttgt cgccgtaaaa aatcgagttc gcgcccgcca taaagcacat
      481 cgcctgcatt gcttcaggca tattgctgcg ccctgccgac agccggacat aactttgcgg
      541 catcgtaatc cgcgccacgg cgatggtgcg gacaaattcc gtccagtcca aatcttcggc
      601 atcggcaagc ggcgtgcctt ccactttgac caaccggtta atcggcacgc tttcgggctg
      661 cgggtcgaga ttggcgagac tggcaatcag cccggcacgt tcggcgcggg tttcgttcat
      721 cccgacgatg ccgccgcagc agactttcaa accggcgttg cggactttgc ccaaggtgtc
      781 cattcggtct tcgtgttggc gggtgtggat gatgtcgttg tagcggtcgg ggtcggtgtc
      841 gaggttgtgg ttgtaataat ccaagcccgc ctctttcaag tcttccgcca taccttcttc
      901 gagcataccg aacgtgccgc aggtttccat acccaagccc ttgacggctt tgatgattgc
      961 ggaaaccgtc tccacgtctt tgggtttagg gccgcgccac gccgcgccca tacaaaaccg
     1021 gcttgcgccg cgcgatttgg cgattttggc tttttcgacg atttcatcca catccatcat
     1081 ctgctctttg cccagattgg tattgtggtg cgccgattgc ggacaatagg cgcagtcttc
     1141 gggacaaccg ccggttttga tggacaacag cgtggaaagc tggatttcgc gcgggttgaa
     1201 attttggcgg tggatttcgg cggcttggta aatgaggtcg aggaagggaa gtccgaacag
     1261 ggcttcgaca tcgcattttt tccaatagcg cgcggtggga tgaggcttgc actcggtctt
     1321 acggcgcaag gcgacggggg atacggtcat aatgtgttct ttcgtattta cagcggcgca
     1381 atgacgctga caggcaaacc gccggcgcaa tgacggcggt tgcgggtaat caaatggctg
     1441 aaaacagcgg cggcaagtgt aacgcgtttg aaaatacggg gcaaacggtt tttccgcctt
     1501 tgtgccgcgc agccgggcga tgccgtctga agggctttca gacggcatcg cccgggctat
     1561 ctcaaagctt ccagcagctt tccgtgtatt ccgccgaaac cgccgttgct catcaccaaa
     1621 atatggtcgc ctacttcggc gtttttcacg atttcggcaa cgaaggcatc gaagtctttg
     1681 ccgacgttca gcctgccgcc caaaggcgcg agggcttcgg cgacgtccca gtccacgccg
     1741 ccggcgtagc agaacacttg gtcggcttct ttgaggctta caggcagggc ggacttcatc
     1801 gtgcccagct tcatcgtgtt ggaacgcggt tcgaggacgg cgaggatgcg cgcgccgccg
     1861 acgcgttggc gcaaaccttg aatcgtggtt tcgatggcgg tcgggtggtg ggcgaagtcg
     1921 tcgtaaacgg tgatgccgtt tgccgtgcct ttgatttcca tccggcgttt gacgttttta
     1981 aacgcgccca aggcttcgca ggcggtctga atatcgacac cgacatgacg cgcggcggca
     2041 atgacggcga gcgcgttcat gcggttgtgc ctgcccatca aatcccattt gacgcgtccg
     2101 gcggttttgc cgtcgagcaa cacgtcgaac gagccgtcgg cattggcttc gccggcctgc
     2161 cagccgtgtt ccgtgccgaa tttttccacc ggcgtccagc agcctttgtc caaagtatct
     2221 tgcaggcttt gctgccgtcc gttgcagacg attaagcctt cagacggcac ggtacgcacg
     2281 aggtagtgga actgggtctg tatcgcgccc aagtcggcaa agatgtcggc gtggtcgaat
     2341 tccagattgt tcaacacggc ggtacgcgga cggtaatgca cgaatttaga acgtttgtcg
     2401 aaaaaggcgg tgtcgtattc gtcggcttcg atgacgaaaa acggcgattg gctgttcggg
     2461 tcttggcgcg gcgtttgcgg caggcgggcg gaaacgccga aattttccgg tacgccgcca
     2521 ataaggaagc ccggcgcgag gccggcatat tccaagaccc atgcgagcat ggaggcggtg
     2581 gtcgttttgc cgtgcgtccc cgccacaccg agtacccaat gatggtgcag cacgttttcc
     2641 gacagccatt gcgggccgga aatataaggc aggccgaggt tcaaaatcgc ttcaaccaca
     2701 tccatcccgc gcttggcgac attgccgata acgtaaacgt cggctttaaa ttcgtccaac
     2761 tgagcggcat cgaagccttc atacacgtct atacccaagg cttcgagctg ggtgctcatc
     2821 ggcggataca tcttcgcgtc gcaaccgctg acttcaaacc ccgcttcttt ggcaatggcg
     2881 gcaagcccgc ccataaacgt gccgccgata ccgataatat ggatgtgttt catcaaagtt
     2941 ccccaatcgt tcaaattttg ccgtccgaca gcgcaagggc tgattcggct tcaatcagtg
     3001 cggcttgatg ccggcagccg aaccgtcttt ctgtgcattt cctttcggat tttggacgtg
     3061 ttcgtccctc accgtgtccg aataatcgcc gacccctgcg tcttccgccc tgtcttttac
     3121 tgccggtcta accgaactgt acacccggtc ccgccccaaa cccgcgttgg tacaatgttt
     3181 tcccggagtt tggctgtaac tggttttacc attatggcgg cacgcgtaaa catttgccga
     3241 aaatacggtc tgaaccgctg taacaagcaa catacttcca attattttag atacgcgcat
     3301 atcaacctcc tttatcgttc ttcttcaaaa aagggaatgt atttcaatag cacttcatct
     3361 aattctttca ttccctgctc ataattgaac gggtattccc aaccatcccc tactttgatt
     3421 tcagaaatat ctttaaactt ccgcttaagg gcggacactt tgtccatatc gtcatcaatt
     3481 tcctgtttgc cgaacggtat tttccgcttt tccaaaacct cgtcataata acaagataaa
     3541 ttaagcaata gcccccaagc actatttgca attacgacta atctatgttt ttccctagtt
     3601 aagtaatctg caccgctatc atagattgcc cttgtcgggt cataaaaaat tcgcaatttt
     3661 tcatagcgtc cccacacttt aaccgattcg caattttata tccgcgattt tgcaagctgc
     3721 ccataaaaac cgattgcctg tcaagacggc actacttgtt aaagatgccc gataatgccg
     3781 caactcttcc gtatttaccg acaaacgccc tatttgctta gagctgtttt gttttccaca
     3841 aaactttaat tcgttgattt aacaaatttt ttaccaaatt ctttatttgt tcggattcca
     3901 ttagcacgat tttgagcaat atctgtcaaa accatttcca ccacacccaa gccaaaaccg
     3961 ccgccgccac ggcattggac aatcagatga gtttccactg cccgtccttc cgcaatgccg
     4021 ccttcttggg cttcaaagca cccgcataaa aaaacggcga cagcagcata gaagcgatac
     4081 ataagaaaat caatacgccg taatagattt tttcagacgg catttctctt cctcaatacg
     4141 ccgcccgttg gcgcatttgt tcaaacaggc agattgtcgc cgccatcgcg acatttaaag
     4201 actcggttgc atcgtgcatc ggtatcctga cacacttgtc cgccctatct aaaactgctt
     4261 taccgacccc cgcgccttcg ttgccaaaca cccaggctgt cggttcgcac aaatcttcgc
     4321 cgtacaaaac cgcctgcttt tcctcgcgca aggcggtggc aaacacacgg cctttatagc
     4381 gcaccaacca tatttccaaa tccgcctgcg gataaatctc cgacaagaaa tgcgcgccca
     4441 ttccggctcg cagcactttg ggcgaccacg cgtccgcaca acctttgccc aaaatgaccg
     4501 cgccgattcc cgccgccgcc gcgcttcgca acaccgtgcc gacattgccc gggtcttgca
     4561 cgccgtccaa aaccacgcaa tcgccgccgg ccggcaaagc acccgcatct ggaatatcaa
     4621 tcagcgcaag cacatcatcc gcacaagtca ggctgctgat tttcttcaat atgccgtctg
     4681 aaacggaaaa aaacccgtct tccggcaaaa ccgccgtcaa tttacggact tcttcagacg
     4741 gcattttcgc ttcgggaata tataccccga ccggcatccc gccggattgc aggaaaacct
     4801 gaagcaggtg cacgccctcc aaaacggttt gggcgtattg ccgtctgaac tttccttgcg
     4861 acaacaggcg gtgcaggtgt ctgatgtgtt cattattggt cgaactgatg tgtttcactt
     4921 cacaagcctt ttagaaatat gcaaccggac aatacccgac tgaacgccgc cgaaatctgt
     4981 ttgcgatatt atcaaaataa aatgggaaag tcaggatgga cgggcaaaat gccgtctgaa
     5041 accgagtatt ggaaatccgc acaacagttt gccaacgcca atggacggtt tccgctgtcg
     5101 gcaacaagcg gccaaattgg gcgcaggaca ggcaaaaccc gtttcccctg aaggattttt
     5161 ttcacatcct tatggatgcc gcccatggcc aacgtatccg atgccgctac cgtcctcaaa
     5221 attccgtcct gctccaaaag atgctcagga agcccaaacg gatgccgctt caacacaaat
     5281 ccctgccccg tcaatatttc ccttaaattt ccgcttatct gcctgtcttt caaaaaccgc
     5341 agtttatccg ttttttccag tcggaacaca aacagcctgc ctgcataatg atgcaattca
     5401 aagccttgca agttccaacg tccggttttt gcctccgtca aaacccgggc aatgtcggca
     5461 agggcattct gattcggcac gggaatgccg ttttccttca gaaaatgccg caaaatatgg
     5521 gttttccggc gcggggaaaa cgtcagccac cgcgccgtat cgaaataacc ggccccgcaa
     5581 acccaacggc agtcctgaac gacgacctcg tccaacaaag ccaaatcttc ctgcaaagcg
     5641 cggacattgt tcagcacatg ccgcccgaaa tggggaatct gcgccgaaag ttcgggcaaa
     5701 atacggtgcc ggaagcggtt tcgcaaataa gccgtatcgg tattgctttc atcctcgata
     5761 ttcggcaaac cgtgtttttg ggcataatcc catatatctt ggcgtgaaaa aggcagcaag
     5821 ggccgccaga tgatgccttt ttccccaaaa gggcggacgg cgggcatagc cgccaaagcg
     5881 cgcaaaccgc cgccgcgcgc gaccgccagc ataaaggttt cgatttgatc gtccctgtgg
     5941 tgcgccaacg ccaaaacgtc aaagcctttt tcggcaaacg cggcataacg cttttgcctt
     6001 gccgccgcct cgatgcccaa accgtttttt tccacgcaga cctt
//
LOCUS       DAWHRA010000124         5934 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWHRA010000124 DAWHRA010000000
VERSION     DAWHRA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.124"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(83..456)
                     /locus_tag="WIB30_001736"
                     /pseudo
     CDS             complement(83..456)
                     /locus_tag="WIB30_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(762..2021)
                     /gene="rho"
                     /locus_tag="WIB30_001737"
     CDS             complement(762..2021)
                     /gene="rho"
                     /locus_tag="WIB30_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ3797769.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     QNVSFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(2241..4625)
                     /gene="ppsA"
                     /locus_tag="WIB30_001738"
     CDS             complement(2241..4625)
                     /gene="ppsA"
                     /locus_tag="WIB30_001738"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ3797770.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGNRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            5036..5857
                     /locus_tag="WIB30_001739"
     CDS             5036..5857
                     /locus_tag="WIB30_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ3797771.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1309 a         1377 c         1662 g         1586 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataaaaa tttatggggc tgtcctagat aactaggata
       61 aactcgattt tactaattgt tttcaaatgg aacaagaact tttatctcac tgttgttaaa
      121 acgccgttcg taccccttta aatacagctc aaaatgcgct ttgggaatgc cgtcaaactt
      181 gcgtaaatga cgttttgccc ggtttcaaaa gttcccaatt ccattgatat ggttttgtcg
      241 ttcagcaaaa taactttcat ctgcttttac ttcgccgtca aacatttcca aatgcggact
      301 gttttgataa ataagtaatc gtaaacgatg aaaataatag gctgcggtac ttttattaac
      361 gcctactaac tctgctgctg ttcttgcagt tacacctgcg acaaacagtt caatgagttt
      421 attttgttta tactggctta gacgactttt tctcataggg gcaactctaa cttaatttga
      481 atttccctag ttatctagga cagccccaaa tttatacaaa aatgagcgcg gttcggcgca
      541 accttgaatc aagttcccgc atcggttttc attgccggta cggatgcgtt caagccggtt
      601 tttcaaaggc cgcgctttcg gcaagcggac acggacactg ccgacggttg cgccgttaac
      661 ggggggaggg aggagctgcg ccgaccgtgt gaatgaaagt gccgtctgaa acccgatttt
      721 caggcttcag acggcatttc gcattaatgc gggcggcgcg tttatttgcc gcgcatcagt
      781 tcaaagaaat cgtcgttgtt tttagaggct ttgattttcc cgtttaagaa ctcggtagct
      841 tcgatttcat ccatcgggtg caggaacttg cgtaagagcc acatacgttg caactggtcg
      901 ttcggcacaa gcagctcttc gcggcgcgtg ccggatttgt tgatgttgat ggcggggaag
      961 aggcgttttt ccgccatacg gcgatcaagg tgcaattcca tattgccggt gcctttgaat
     1021 tcttcgtaaa tcacatcgtc catacggctg ccggtttcaa ccaatgcggt ggcgataatg
     1081 gtcagcgaac cgccttcttc cacgttgcgc gccgcaccga agaaacgttt ggggcggtgc
     1141 agcgcgttgg catcgacacc gccggtcagg attttgcccg aggtggggac gacggtattg
     1201 taggcgcggg caaggcgggt aatcgaatcc agcaggatga ccacgtcttt tttgtgttcc
     1261 accatacgct tggctttttc aagcaccatt tcagcaactt ggacgtggcg ttgcgccggt
     1321 tcgtcaaagg tggaggaaac cacttcgccg cggacggaac ggctcatttc ggttacttct
     1381 tcgggacgct cgtcaatcaa gaggacgatg agttcgactt cgggatagtt tgcggtaacg
     1441 gcgtgggcaa tattttgcag catcacggtt ttaccgcttt tgggcggggc aaccaagagg
     1501 gcgcgctgac ctttgccgat gggggaaatc aggtcgatgg cgcgtccggt caggttttct
     1561 tcggacttta agtcgcgttc cagcttcaac tgttcggtcg gaaacagcgg ggtcaggttt
     1621 tcaaacagga ttttatggcg gcatacttcc gggtggtcgc cgttgatggt atcgaggcgg
     1681 acaagcgcga aataacgctc gttgtcttta ggcacgcgca cgctgccttc gatggtgtcg
     1741 ccggtgtgca gattgaagcg gcggatttgc gtgggcgaaa cataaatatc gtcagggccg
     1801 gcaaggtaag aagtatctgc gctgcgcagg aaaccgaagc cgtcaggcag gatttctagc
     1861 gtaccggaac aggtaaagct gacattctgt ttcatcatct ggcggacgat ggcaaaaacg
     1921 aggtcttgtt tgcggaaacg gttggcgttt tcgatgccgt gttcttccgc caattctaag
     1981 agtttggaaa tgtgcagggt ttgtaattcg gagacgtgca taataatgat gtattttgaa
     2041 gaggaaaaag acaggcagat gccgtctgaa agaagaagct gaccgttgcc ggttgctcgg
     2101 ggaaagggga attgtaggca gtcggcgcgt gggtgtcaaa tattatcgcg gacggggcat
     2161 cggcaggaaa tgccgtctga gcggagctgc ttggaaaaaa atacccccgc gcttttcagg
     2221 ctcgggggta tgggcattga ttatttgttc aattcattcg ccaaatacag ccaagtttcg
     2281 atgacggtat ccgggttcag ggaaacgctt tcaatgcctt cctcaaccag ccatttggcg
     2341 aagtccggat ggtcggacgg gccttgaccg cagatgccga catatttgtt ctgcttgcgg
     2401 caggcggaga tggcaaggtg cagcatcact ttgacggcag ggttgcgttc gtcaaacgat
     2461 tcggatacca agccgctgtc gcggtcgaga ccgagggtca gttgggtcat gtcgttcgag
     2521 ccgatggaga agccgtcgaa gtattgcagg aattgttccg ccaataccgc gttgctcggc
     2581 agctcgcaca tcataatcag gcgcaggccg tttttgccgc gttccaagcc gttttctttc
     2641 agggctttga caacggcttc ggcttcgccc aaggtgcgga cgaacggaat catgatttca
     2701 acgttggtca accccatttc atcgcggacg cgtttcaagg ctttgcattc caaggcgaaa
     2761 cagtctttga agttgtcggc gacataacgc gccgcgcccc ggaagcccaa catcgggttt
     2821 tcttcatgcg gttcgtatac gttgccgccg accaggttgg cgtattcgtt ggatttgaag
     2881 tcggacatac ggacgatggt tttacgcgga taaaccgatg cggccaaagt tgccacgcct
     2941 tctgcgattt tatcgacgta gaagtcgaca ggggacgcat agccggcgat gcggcgggta
     3001 atttccgctt tcaattcgtc gtcttgtttg tcaaattcca acaaggcttt ggggtggata
     3061 ccgatttggc ggttgatgat aaattccata cgcgccaagc cgatgccttc gctgggcagg
     3121 ttggcgaagc tgaatgcgag ttcgggattg ccgacgttca tcatgacttt aaccggtgct
     3181 ttgggcatat tgtctaaggc gacatcggta atctgtacgt ccaacagacc ggcatagata
     3241 aagccggtat cgccttcggc acaggatacg gtaacttctt gaccgttttt cagcaattcg
     3301 gttgcattgc cgcagccgac aacggcagga atgcccaatt cacgcgcgat gatggcggcg
     3361 tggcaggtac ggccgccgcg gttggtcacg atggcagaag cacgtttcat cacgggttcc
     3421 caatccggat cggtcatgtc ggtaacgagt acgtcgccgg cttcgacgga atccatctcg
     3481 gaagcatctt taatcaggcg caccttgccc tgaccgactt tctgaccgat ggcgcggcct
     3541 tcgcataata cggttttgtc gccgttgatg gcgaagcggc gcaggttgcg gttgccctct
     3601 tcttgggatt ttacggtttc gggacgggct tgcaggatgt agagtttgcc gtccaagccg
     3661 tcgcgtcccc attcgatatc catcgggcgg ccgtagtgtt tttcgatggt cagtgcgtaa
     3721 tgcgccaact cagtaatttc ttcgtcggta atggagaagc ggttgcggtc ttcctcgggg
     3781 acatcgacgt tggttacgga tttaccggct tctgctttgt cggtaaaaat cattttgatg
     3841 tgttttgaac ccatggtttt acgcaggatg gcgggcttgc ccgctttgag cgtgggtttg
     3901 aacacataaa attcgtccgg gttgaccgca ccttgtacga cgttttcgcc cagaccgtaa
     3961 gaggaggtaa caaagacgac ttgatcgtag ccggattcgg tgtcgagggt gaacatcaca
     4021 cctgatgcgc cgctgtcgga acgcaccatg cgttgaacgc cggcggaaag ggcgacgatg
     4081 tcgtgttcga agcctttgtg gacacggtaa gaaatggcac ggtcgttata cagggaagcg
     4141 aatacatggt gcatcgcttc tttaacgtta tccaagccgt tgatgttcaa gaaggtttcc
     4201 tgttgtccag cgaatgatgc gtccggcagg tcttcggcag ttgcggaaga acgtacggca
     4261 acggaaatgt ccgcaccgcc ggcatcggca accattttgt tccatgccgc ttcgatttcg
     4321 gcatcgagct gttcggggaa aggcgtatcc aaaatccatt ggcggatttc tttgccgacg
     4381 cgtgccagtt cggcaacgtc ttcgacatcc aattttgcca gtgcggcgga aatgcgttcg
     4441 ctcagaccgt tgtgtgcgag gaatgcgcgg taggcatcgg ctgtggtggc aaagccgccg
     4501 gggacgcgaa cgcctttttc ggtcagctga ctgatcattt cgcccagcga ggcgttttta
     4561 ccgcccacgc gttcaacatc tgtcatacgc aggttttcaa accagattac gtagttgtcg
     4621 gccatttgtg tgtccaatcc aaaatatgtt aaaaaagaaa caaatccgcg tgcttatttt
     4681 aagcgattcg ttcctctgct gtcatgtgtt ttatccattt taaaatcatg atgccgtctg
     4741 aaaaattgcg gtttcggcgt gtgtagcggt ttgaaacttg cagccggtat acttcttttt
     4801 ttgggtattt tctttgtaaa acaggtggtt tgaataggtt aatgtttttt ctgtttgatt
     4861 tttttgttta ttttttaaaa ttttctgcca aaaaatactt tatataaata attttatttc
     4921 aaaattatat tgtgtctgtt tgggtgtaat ccgaggtagg cgtgctgcgg gtgctttcct
     4981 tgtgtctgcc gctgctgtta tgatgatatt tttattctgt atttaaggat ggaagatgag
     5041 cagtccgcgc catgtgtttt acatttccga ccgtaccggt ctgactgccg agaatatcgg
     5101 cgaggctttg ctgaaccagt ttggcaatct gtcgttcaaa cgccatacgc atccgtttgt
     5161 cgatacgccg gaaaaggcgc gcgcggtggt ggagaaggtc aatcggagcc ggcaggaaaa
     5221 cggtcagcgt ccgattgcgt ttgtcagtgt ggttgatgac gaaatccgtc ggattatcaa
     5281 aggggcggat gcttttcaga ttaatttctt tgagactttt ttgggactgt tggagaaaga
     5341 actcaatacc gaagccacgg catccgagca ggggcatcac agtatcggta atacgaagcg
     5401 ttacgatgcg cgtatggagg cggtgaattt ctcattgaac cacgatgacg gggtcagcga
     5461 taagaacctt caggaagcgg atgtaatctt gatgggtgta tcgcgttcgg gcaaaacgcc
     5521 gacctgcctt tacctcgccc tgcaatacgg catccgtgcg gcaaactatc cgctgattcc
     5581 cgacgatttg gaatcggccg atctgccgcg tatggtcaag ccttataggg ataagctgtt
     5641 cgggttgacc atccagccgg aacgtttgca ggccatccgc caagagcgcc gcccgaattc
     5701 aacttatgcc aaaatcgata catgccgcag cgaggtggcg gacgcgcaga gtatgttcag
     5761 acggcatggc attccgtttg cgaatacgac ggataaatca gtggaagagc ttgccgtaca
     5821 catccttcag gcgtgcaagc tcaaacgcag gttttgacgg gctttgattc ggtttgaagg
     5881 cggaaatgcc gtctgaaatc aggtttcaga cggcagtttt atagtggatt aaca
//
LOCUS       DAWHRA010000125         5914 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWHRA010000125 DAWHRA010000000
VERSION     DAWHRA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.125"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(321..905)
                     /gene="rnhB"
                     /locus_tag="WIB30_001740"
     CDS             complement(321..905)
                     /gene="rnhB"
                     /locus_tag="WIB30_001740"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ3797772.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVEWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(974..2869)
                     /gene="mnmG"
                     /locus_tag="WIB30_001741"
     CDS             complement(974..2869)
                     /gene="mnmG"
                     /locus_tag="WIB30_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ3797773.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAATRMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLEHYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVSEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEGEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3194..4621)
                     /locus_tag="WIB30_001742"
     CDS             complement(3194..4621)
                     /locus_tag="WIB30_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ3797774.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            4781..5773
                     /gene="pdxA"
                     /locus_tag="WIB30_001743"
     CDS             4781..5773
                     /gene="pdxA"
                     /locus_tag="WIB30_001743"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ3797775.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGASEEGVLEVLHIPAAEAVEAGKLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLNKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
BASE COUNT         1216 a         1559 c         1739 g         1400 t
ORIGIN      
        1 ttttgccagc cctgagactc cggattcctg ttttcgtagg aatccggttt tttgagcttc
       61 agtcattttt gataaattct tgcagctttg agtttctaga ttcccgcttt cgcgggaatg
      121 acggtttgga agttacctga aattcaaaaa aaaaaacgga aaccggacgg attggattcc
      181 cgcctgcgcg ggaatgacgg attttaggtt tttttttgat tttctatttt tcgcgggaat
      241 gacggtttgg gttctttctc tttggagttg cgatgccgga aatgccgtct gaaggcttca
      301 gacggcattt ttgtgccggt ttaaaacaag gcctgctgcg cgagcaggtt tctgacgggg
      361 gcgaagtcgc ggcggtgttc gggcagcacg ccgtattttt cgagggcttc caaatgctgc
      421 ttcgtgccgt aacctttgtg tttgtcgaaa ccgtattggg gatggcgttg cgccagtgcg
      481 tacatttccg catcgcgtgc ggtctttgcc aaaacggatg cggcggagat ttcgatgatt
      541 ttgctgtcgc ctttgacgac ggcttcggca gggatgttca aatgttcagg aatgcggttg
      601 ccgtcgatga atattttttc gggacgcaca gccaagccgt caacggcgcg tttcatcgcg
      661 agcatggtgg cgtgcaggat gttgaggctg gcgatttctt cgggcgaggc ggcggcaacg
      721 tgccactcaa ccgcctgatt ttttatcatt tcggcaagcg cgtcgcgttt tttctcgctg
      781 agttttttgg agtcggtcag tccgggcagg tcgaatgttt ccggaaggat gacggcggcg
      841 gcaaacacgc tgccgactaa aggtccgcgt cctgcctcgt ccacgccggc ggtcagtatg
      901 tgcatgatgt ttcctgtcgg gatggtggga atgccgtctg aaaagggttt cagacggcat
      961 cgcgccgatg tgtttatttc gcgtctttaa acccgcgctt caaatgcacc atcagcaatg
     1021 ccactgccgc aggggttacg ccggaaatgc ggctggcttg tccgacggtt tcgggtttgt
     1081 gctggttgag cttttgctgc acttctgccg acaagccttt gactttgccg taatcgatgc
     1141 cgtcgggcag ttttaaggtt tcgatgtcgc ggcggctgtc gatttcttcg ttttggcggt
     1201 cgatatagcc ttggtatttg acttggattt cgacttgttc gatgacttcg gcggagaggt
     1261 tttcagacgg catcgcgcct tcgagcgtca tcagcgcggc gtagtcgagg tttgggcggc
     1321 gcaggaggtc gtgcaggttg gcttcgcggc tgagtttttg tccgaacaca cggatttgtt
     1381 cgccttcggc gagtttttgc ggcgtgtacc acgttgtttt caaacgttgg atttcgcgtt
     1441 cgacggcttc gcgtttttcg ttgaacatgc gccattgcgc ttcggacacc aagccgattt
     1501 tgtagccgtc ttcggtcagg cgcatgtcgg cgttgtcttc cctgagttgc aggcggtatt
     1561 cggcacggct ggtgaacatt cggtagggtt cgttcacgcc tttggtgatg aggtcgtcca
     1621 ccaatacgcc gagataggct tgttcgcggc gcagcaggag cgggtcttgt tcgcggacgt
     1681 attgcacggc gttcgcgcct gccagcagtc cttgcgcggc ggcttcttca taacctgtcg
     1741 taccgttgat ttgcccggcg aaaaacaatc cggcaatggt tttggtttcg aggcttgctt
     1801 tgaggttgcg cggatcgaag tagtcgtatt cgatggcgta gccggggcgc aggatatggg
     1861 cgttttccaa acctttcata ctgcggacga gcgcgatttg gatgtcgaac ggcaggctgg
     1921 tggagatacc gttggggtag tattcgtgcg tggtcaggcc ttcgggttcg aggaaaatct
     1981 ggtggctgtc tttgtcggcg aagcggttga ttttgtcttc gatagacgga caataacgcg
     2041 ggcccacgcc ttcgattttg ccggtaaaca tcgggctgcg gtcgaagcct gagcggatga
     2101 tgtcgtgggt ttgcgtgttg gtatgcgtaa tccagcagga cacttggcgc gggtgcattt
     2161 cggcgttgcc gcgtacggac atgacgggaa cgggcgtgtc gccgggctgt tcggtcagtt
     2221 gggagaagtc aatcgtgcgt ccgtcgatgc gcggcggcgt gccggttttc agacggcctt
     2281 gcggcagctt caattcgcgc aaacgtccgc ccagcgattt ggcggcgggg tcgccggcgc
     2341 gtccgccttc gtagtgttcc aaaccgatgt ggattttgcc ggacaaaaac gtgcctgcgg
     2401 tcaacacgac ggcgcgtgct ttaaactcca cgcccatcgc ggtaattacg ccgctgatgc
     2461 gttcgccgtc gagcgttacg tcttcgacgg cttgttggaa aaggtcgagg ttttcttggt
     2521 tttccaacat ttcgcggatg gcggctttat acaaaatgcg gtcggcctgt gcgcgcgtgg
     2581 ctcgcactgc cgcgcctttg ctggcgttca ggcggcggaa ttggatacct gatttgtcgg
     2641 ttgccaacgc cattgcgcca ccgagcgcgt cgagttcgcg taccaaatgc cctttgccga
     2701 tgccgccgat ggaggggttg cacgacattt gtccgagcgt ttcaatattg tgtgtgagca
     2761 aaagcgtctg cgcgcccatg cgtgtggcgg caagcgcggc ttccgtgccg gcgtgtccgc
     2821 cgccgacgac gataacgtcg taggttttgg ggtaaatcat gtgggtcatg tgtgtattgc
     2881 ctgtcggtgt ttcagacggc atttatagtg gattaacaaa aaccagtaca gcgttgcctc
     2941 gccttagctc aaagagaacg attctctaag gtgctgaagt accaagtgaa tcggtttcgt
     3001 actgcttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaaa ccactatatt
     3061 caatatgccg tctgaaaaac gaaatggatt caaaagtaaa gggttgggat tgtacgcttg
     3121 ttcgccctgt ttttacagtg tgcggaaagg gaaaagccgc ttcgcgggga agcggctccg
     3181 gtaagggcgg gatttaccaa acgtcggatt tgatacggcg tttcaggccc ggatgttcgg
     3241 aaagtttgaa ctcggggtct ttgcccattt tcagcttggc ggtgtaatcg cgcagcagca
     3301 taaacgccaa gggcgagagc agcaggatgg cgacaaggtt gatccacgcc ataatgccca
     3361 tcgccatatc cgccatatcc cagaccaaag gcacattggc aaccgcgccg aaatagaccc
     3421 acgccaaaac cagcatacgg aaaacggcgg taatcagcca atggcttttg atgaattgga
     3481 cgttggactc ggcataggca tagttgccga taacggtgga aaaggcaaac ataaacagga
     3541 tgacggcgag gaagcccgcg ccccattgcc ccacttggct gacaatcgcc gcctgcgtca
     3601 gcgccgcacc gctcaaatcg ccgtaaggct gttggtaaat caagatgatg aaggcggtgc
     3661 aagaacaaac gatgatggta tcgacaaaca cgcccagcat ttgaatcata ccttgcgaaa
     3721 cagggtgttt cacttcggcg gcggcggcgg cgttcggcgc ggaacccata cccgcctcgt
     3781 tggaatacag gccgcgtttg atgcccatca tcatcgtttg cgaaatcaga ccgccgagta
     3841 agccgcctgc tgccgcgtcg aatttgaacg cgcccgaaaa aatctgaccg aacacgtccg
     3901 gaatcatcgg aatattggtc aaaatgatga aaagcgcgat aaagaggtac aaaaccgcca
     3961 tcagggggac gacgatttcc gccgctttag atatgcgcct gatgccgccg aagataatcg
     4021 gcgcggttaa aatcaccagg gcgacgccga cataatgagg ctcccaaccc catgccgctt
     4081 tgacggtatc ggcgatggta ttggtctgaa ccgcttcaaa cacaaagccg aaacagaaaa
     4141 tcaggctcag ggcgaacaac acgcccagcc atttctgccc cagcccttga gtgatgtagt
     4201 aggcagggcc gccccggaaa tggtggttgt cgtagtcgcg gactttaaag agctgcgcca
     4261 gcgaagattc gacaaacgcc gaactcatac cgattaaggc ggttacccac atccaaaaca
     4321 ccgcgcccgg tccgccgact ttgatggcga tggccacgcc cgcgatattg cccacgccca
     4381 cgcggctggc aaggccggtt acaaatgcct gaaacggcgt gatgccgtga gggtcgtccc
     4441 cctgtttgcg gccgccgagc atttctttga tgctgcgccc gaacaggcgg aattggacaa
     4501 agcccgtggt tacggtgaag aaaagccccg tacccaaaag catataaacc aagtatgacc
     4561 acatcggatc gttgatggcg ccgacccagc cgtgcagcca ttcggtaaag ttctcgttca
     4621 tatcgcttcc ttaaagttga aactcgcaca tattggcggt atgcaagcag ggtttaaatt
     4681 ttgtaaacgc ccattctagc agattgtcaa caaaatcaga aaaatttaca tcgccgcgcg
     4741 gctgcggcgt tagaatcgca ttttgtttgg agcaaacacg atgaaacagc ctgtttttgc
     4801 cgttacttcc ggcgagcctg ccggcatcgg ccccgatatt tgtttggact tggcatttgc
     4861 acgcctgccc tgccgctgcg cggtattggg cgacaaaaac ctgttgcgcg cgcgcgccga
     4921 agccttgggc aaaagcgtcg tcctgcgcga cttcgatccg gaatcaggcg gcgcgtccga
     4981 agaaggcgtg ctggaagtgc tgcacatccc tgccgccgaa gcggttgagg cgggcaaact
     5041 caatcccgcc aacgccgcct atgtgctgca acttctagac accgcgctcg caggcatttc
     5101 agacggcatt ttcgacggca tcgttaccgc gccgctgaac aaaggcatca tcaacgacgc
     5161 gcgcgcaagc acaggttttt tcagcggaca caccgaatat ctggcggaaa aaagcggcac
     5221 ggggcaggtt gtgatgatgc ttgccggcaa aggcctgcgc gtcgccctcg taacgaccca
     5281 cctgccgctg aaagacgttg ccgccgccat cacgcaaccg ctgattgaaa gcgtcgcacg
     5341 cattttgcat cacgacttaa aacacaaatt cggcatcaaa aatcccaaaa tcctagtcgc
     5401 cggactcaac ccccacgccg gcgaaggcgg acacctcgga cacgaagaaa ccgacaccat
     5461 tatccccgcg ctcgaaaacc tgcgccgcga agggataaac ctcgccggcc cgtatccggc
     5521 ggataccttg ttccaaccgt ttatgctcga aggtgcggat gccgtattgg cgatgtacca
     5581 cgaccaaggg ctgcccgtgt tgaaatacca cagcttcgga cagggcgtga acatcacgct
     5641 cggcctgccc tttatccgca cctccgtcga tcacggcacc gcgcttgatt tggcggcaac
     5701 cggcagggcg gattccggca gcctgataac tgccgtggag accgccgtcg agatggcgcg
     5761 cggcagcctt taaagatgat aaaagacccg tcatttccgc gcaggcggga atccggtctg
     5821 ttcggtttca gttgtttttg ggtttcgggt aatttccaaa tcgtcattcc cgcgcaggcg
     5881 ggaatccaga ccattggaca gcggcaatat tcaa
//
LOCUS       DAWHRA010000126         5862 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWHRA010000126 DAWHRA010000000
VERSION     DAWHRA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.126"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            52..1005
                     /locus_tag="WIB30_001744"
     CDS             52..1005
                     /locus_tag="WIB30_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ3797776.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1110..1433)
                     /locus_tag="WIB30_001745"
     CDS             complement(1110..1433)
                     /locus_tag="WIB30_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797777.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVEIDLFGSNSTMYVCSITPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1606..2967)
                     /locus_tag="WIB30_001746"
     CDS             complement(1606..2967)
                     /locus_tag="WIB30_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ3797778.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEVIEVTTFRGGAKVHQNARGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGMDDIAERKLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSQATAGPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3399..5099)
                     /gene="recJ"
                     /locus_tag="WIB30_001747"
     CDS             complement(3399..5099)
                     /gene="recJ"
                     /locus_tag="WIB30_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ3797779.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDTEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLAVYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVREDDLSQTFITDGSLKACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5168..>5862)
                     /locus_tag="WIB30_001748"
     CDS             complement(5168..>5862)
                     /locus_tag="WIB30_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797780.1"
                     /translation="AAVLAATLMLNGCTAMMWGMNDPFSQTTAYKHVDKDQIRAFGVV
                     AKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPRFAYQALP
                     VKLESPASQNFSTDDLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCVSAKGKYY
                     ATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAAGAVLVLPM
                     AALIAAANSSDK"
BASE COUNT         1346 a         1670 c         1495 g         1351 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt cgaccgtgtc
      661 accaagctga ttgaaaaagg tctgattgag attgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacgga tacggaagcc aagctccgta
     1081 tccgtttctt tatccctcca accgatacat caatcgaatt ttctgcatcg gattgcctca
     1141 tcttcgcaaa acattgccga agtttcgcgg ttgcacgcct gacgcacttt ctgccgcgcc
     1201 acgccttcat tccggtcgct tgcctcaaac gtcttctgaa aaggcgtaat gctgcataca
     1261 tacatggtgg aatttgaacc gaaaaggtcg atttcgacgg tattgccgcc gctttccaat
     1321 acggcgacac ggtgttccaa ctgcgccaga cgtgcttcca aatcgctgat tctgtcggca
     1381 aatgcaggca tggcggcaca cgcggtcaaa atgcccaaaa tacatttttt catcaatttt
     1441 tccttgttgc ttgaaccctg cccacccgga catccttttt ccgagaggca gggtcagact
     1501 ttatttacgg ggatgaagcc ccttacaaat caatataata catggaacgg cactttatgc
     1561 ttcatcccat gtattgaaac ttcattgctc cttgtttgtt gaccgttatt cccaatccgt
     1621 tcccacaacc ttcggcttgg gtttgcgcct gcgacggcgg cgttttttcg cctgtccttc
     1681 gtttttttcg tgccgggcgg cagcctcgtt tttggtcatt tccgtccgct gttccgtcga
     1741 tgccgtctga aacgccgtcc accactcggc aagggcgcgg tccgcattgc cggtttcggc
     1801 gcgcaagagc aggaaatcat aggcggcacg gaaacgcgcc tgtgcaaaca gtttgtgcgg
     1861 acgtgcgcct ttgcggtttt caaactgcgg ctgaaacatc caaatttcgc gcatcgtggc
     1921 ggaaaagcgt tgcggcacgc cccaaccgcg ttcgacggtt tcacgcatcg tattgattgc
     1981 atcggacagg gcgggcgcgg gtttcaaacc ctgttgcaga ttgcttttcc aatggcgttc
     2041 caactcgggc cacatcagag ccgccagtac aaaaccgacc gaaaccgatt tgtcggcacg
     2101 cagccgctca tcggtatttt tcagggcaag caccgtcatt tttccggcga tgccgtctga
     2161 aacgcgcaag gcattgagca gcggatggat gtcgtccggt atgtcaaatc cgttcaaacg
     2221 tttcagacac tcgcgcgcgt gccctgaaaa cagcagtttc atgatttcgt caaacagcct
     2281 cgctaccggt tcgtgcttca gacggcatat tgattcggca ataggtccgg cggtcgcttg
     2341 cgacaattcg aatcccaatt tccccgacag gcggattgcc cttaaaatcc tgacagggtc
     2401 ttcctgatac cgttcggcgg cattaccgat cattaccagc ttccgttcgg caatatcgtc
     2461 cataccgttg tggaaatcca aaatttcttc tttttccgga tcgtaataca acgcgttgca
     2521 ggtaaagtcg cgcctcatgg catcttcttc gatactgccg taggtattgt ctttcataat
     2581 cctgccgcgt gcattctgat gtactttcgc accgccccga aaagtcgtta cctcaatcac
     2641 ttccgcaccg ttcatcacat gaacgatttg gaaacgcctg ccgatgatgc ggctgcggcg
     2701 gaagagtttg tgcacctgtt cgggcgtggc atcggttgcg acatcgaaat ccttgggttc
     2761 gatgccgagc agcaggtccc tgaccgcgcc gccgaccaca taagcctgaa accccgcccc
     2821 tttcaggcgg cgtatgacgt tttcggcggc aaagctcaac atttcggcac ggatgttgtg
     2881 tctttcggca ggaatgatcg ttttactttc cgctttttta ctgctccgac cggaaggcag
     2941 catcttattt aaccattttt tcagcataat attctaaccc gaagagcggc atctccacgg
     3001 gcaaccgtgt tcagacggca tcgggcgcgg tttgcaagat gccgtaacca taaacaaaag
     3061 gcgtagaaac tacgccgtcc gaatcacgcc gccctcgcgg cggcaacgcg tcattataac
     3121 agattgccct ccaaaaaaat cagaccctgc ttttgtggca gagtctgaaa tttgatgtgc
     3181 cttctccgat tgggaacatt cccgtcgtac aaacaggcaa atgtttcaac acacaggacg
     3241 acacataaag caccgtccta tgtgttgccc tgatttggaa ggggttacgc tccctcccaa
     3301 ataaggtctg attctaccgc cctaaagggt ggggtttcaa ccgaaaagga aatacgatga
     3361 aggaacagaa atcacattca aacggcgttc tgccgcctct acgccgcttc ccagtagtcg
     3421 atataaagct gcaattcgag attgttgcgc cattcgttgg caacggggcg gtaaaccgtg
     3481 cggatgtatt cggggatgtc ttcgctgcaa cgccaaaaca tggcttcaaa ttcgcagccg
     3541 tctttctgca gccacgcttt tttgtgttta ccctccgcgc ccaaaggctg ctggcggacg
     3601 acgtggaact cgtcggtaaa gcttggcggc gcgaagccct gcccccaaac gtgtcgggca
     3661 aggttttgag cctgttccag cgtgatgtcg caggctttca ggctgccgtc ggtgatgaag
     3721 gtttgcgaca aatcgtcttc gcgcaccatc tcgcgcacgg cttcttcaaa ggccgtctga
     3781 aacgcgggaa tattgtgttc aagtatgctc aagcccgccg ccattgcgtg tccgccgaat
     3841 ttcaaaatca aatcgggatg gcgtttggac accaagtcca aagcatcgcg caggtgcaaa
     3901 ttgggaatgg agcgtcccga accgcgcact tcgccgttgt ccgccggagc aaacacgatg
     3961 gtcggacgat aaaaacggtc tttgaggcgg ctggcaacga tgccgaccac gccttgatgg
     4021 aagtcgtcgc gatacaccgc caaagtcgtc tgacctgaag gcagggtttc aggaaaagca
     4081 ttcagcgcgt cttgcagcat agactgctcg atttcgcgac gctcgatatt gaggttgttt
     4141 aactgcgccg ccagttcctg cgcctcggta tcatctcgcg ccaacaggca ggcaataccg
     4201 accgacatat cgtccagccg tccggcggcg ttgatgcgcg ggcccaacgc aaaacccata
     4261 tcaaacggct gcgccttgcg ccaatcgcgc cgcgccactt caaacaaggc gcggataccg
     4321 gggcgcattt tgccggagcg catccgtttc aaaccttgcg acacgaggat gcggttgttg
     4381 tggtcgagag ggacgacatc ggcaaccgtg ccgagtgcga ccaaatccaa aagttcgccc
     4441 agattcggct ctttgatgcc gtctgaaaaa taattgcggc ggcgcaattc ggcacgcaac
     4501 gccatcaata cataaaaaat cacgcccacg cccgccaagc ttttgcttgg aaaaccgcag
     4561 cctttttggt tcggattgac gatgatgcag tcgggcacgg tctcggcagg cagatggtgg
     4621 tcggtaacga tgacatccaa acccaattcc tgcgccctcg ccacgcctgc gatgctggcg
     4681 ataccgttat caaccgtaat cagcaaatcc acaccttgct ctgccgcgat ttcagccagc
     4741 tcgggcgtta agccgtagcc gtgttcaaag cggttgggca cgaggaaatc cactttcgcg
     4801 cccatcgccg ccaaaccgtc caaaccgacg gcacacgccg tcgcgccgtc ggcatcatag
     4861 tcggcgacaa tcaggatttt ttcctgacgc tcaaccgcat ccgccaaacg gcgggcggcg
     4921 gcttcgcaat tcgtcagcga ttgataaggc aggagggaag cgagtttgtc gtccaattcg
     4981 gcaggacttt gcacaccgcg cgaagcacaa agccgggcga ttaaaggatc ggcaccggcg
     5041 gtgagcaaat gattaaaaac gtcggtattg acggagcggg tttggatttt gacggacata
     5101 acgtggaact ttacagtgcg ggtgtgcttg aaatgccgtc tgaaggtttt gcagacggca
     5161 ttgccgttca tttgtctgag gaattcgcgg ctgcaatcaa cgccgccata ggcaagacca
     5221 gtaccgcacc cgccgcatcc agtatcaacg tggtgggcgt atatgcaata ttttcaaaca
     5281 acttggattt gtcggtatgt tttttcgtaa ccgtgtaata aatatcggca ggcacacttt
     5341 gctcaaaatg ataatcggcg ttcagttttt gcggtgtggc gtagtatttg cctttggcgg
     5401 agacgcagcg cgtgtaaatg gtccgattgt cgagttcgac cgcttcaaac tcaagctgtt
     5461 tcagcttggc gatgtcggca ggtctgtcgg tatcgtagcg caggcaaagg tcgtcggtac
     5521 tgaaattctg gctggcgggc gattcgagtt tgaccggcag ggcttggtag gcaaagcgcg
     5581 ggttgggctc aaccatttga aactgcttgt ccaacccggc cttcaaaatg cccgtcagct
     5641 tcgccgaatc ttcaggattg acgacgaacc agtatttccc gcccatcatc accaggctgc
     5701 ccttttccaa ttgggcattg tctttggcaa ccacaccgaa ggcgcggatt tggtctttgt
     5761 caacgtgttt ataggcggtc gtttggctga acgggtcgtt catgccccac atcattgccg
     5821 tacagccatt cagcatcaag gttgccgcca aaacggcggc gg
//
LOCUS       DAWHRA010000127         5602 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWHRA010000127 DAWHRA010000000
VERSION     DAWHRA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.127"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            38..643
                     /locus_tag="WIB30_001749"
     CDS             38..643
                     /locus_tag="WIB30_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ3797781.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            715..1107
                     /locus_tag="WIB30_001750"
     CDS             715..1107
                     /locus_tag="WIB30_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ3797782.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(1136..1285)
                     /locus_tag="WIB30_001751"
     CDS             complement(1136..1285)
                     /locus_tag="WIB30_001751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797783.1"
                     /translation="MPSEARIAGSDGIFNIPISNRQGQHGFDTPKRVLPINRFRLRPI
                     GQNAV"
     gene            complement(1306..1752)
                     /locus_tag="WIB30_001752"
     CDS             complement(1306..1752)
                     /locus_tag="WIB30_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ3797784.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTDAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTE
                     VEAAVAETGAAGMADMGKVMGLLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(1884..2096)
                     /locus_tag="WIB30_001753"
     CDS             complement(1884..2096)
                     /locus_tag="WIB30_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ3797785.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(2385..4235)
                     /locus_tag="WIB30_001754"
     CDS             complement(2385..4235)
                     /locus_tag="WIB30_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ3797786.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVMGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            4612..5349
                     /locus_tag="WIB30_001755"
     CDS             4612..5349
                     /locus_tag="WIB30_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ3797787.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFTVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSDIGRELIRED"
     gene            5351..>5602
                     /locus_tag="WIB30_001756"
     CDS             5351..>5602
                     /locus_tag="WIB30_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ3797788.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMI"
BASE COUNT         1145 a         1586 c         1533 g         1338 t
ORIGIN      
        1 gccgttttgc aggcatcaca caggagcgac aattattatg atgaccctct attccggcat
       61 tacctgcccc ttcagccacc gctgccgctt cgttttgtac gaaaaaggta tggattttga
      121 aatcaaagac gtcgatattt acaacaaacc cgaagacctc gccgtcatga atccgtataa
      181 ccaagttccc gtgctggtcg agcgcgattt ggtgctgcac gagtccaata tcatcaacga
      241 atacattgac gaacgcttcc cccatccgca gctgatgccc ggcgatcccg ttatgcgcgg
      301 tcggggccgg ctggtgctgt accgtatgga aaaagaattg ttcaaccacg tccaagtgtt
      361 agaaaacccc gccgccacca acaaggaaca ggcaaaagcg cgcgaagcca tcggcaacgg
      421 tctgaccatg cttgcccctt cgttcagcaa aagcaaatac atcctcggcg aagatttttc
      481 tatgattgat gtcgcccttg ctccgctgct gtggcggctc gaccactacg atgtcaaact
      541 gggcaaaagt gccgcgccgc tgctcaaata cgccgagcgc atcttccagc gcgaagcctt
      601 tatcgaagca ctgacacccg ccgaaaaagc catgcgcaaa taagtccgaa atgcttgcaa
      661 aacccaccgt tttgcaggca ttttcctatt ttggcgtaca acacggaacc cattatgccc
      721 acttccacca aaccctacat cctccgcgcc ctctgcgaat ggtgcagcga caacagcctc
      781 acaccgcaca tccttgtctg ggtcaacgaa cacacgcgcg tccccatgca gtacgtccgc
      841 gacaacgaaa ttatgctcaa catcggcgcg accgccacgc aaaaccttca aatcgacaac
      901 gattggatca gcttttccgc ccgcttcggc ggacaggcgc acgatatatg gatacctgtc
      961 ggacacgtcc tcagcctttt cgcacgggag accggagaag gtatggggtt tgagttggaa
     1021 gcgtaccgcc ccgatacgcc gcctgaaaac acctctgccg aaaccgcgcc ccgacccgcc
     1081 aaaaaaggct tgaaattggt caaataaatc tatgccgtct gaacggaatc gtgtttcaga
     1141 cggcattttg tccgatgggg cgcaaacgga atctgtttat cggcaaaacc cgtttcggcg
     1201 tatcaaaacc gtgttgcccc tgccgattcg atattgggat attgaaaatg ccgtctgaac
     1261 ctgcgatacg ggcttcagac ggcattttgt ccgatattcg ggcaatcagg cggtcagcac
     1321 ggctttcagg attttgttga cttcgcccat gtcggcttta cctgcgaggc gggttttcag
     1381 cagccccatg actttaccca tatccgccat acctgccgcg ccggtttcgg caacggcagc
     1441 ttcgacctcg gtacggattt cgccggcgga aagcatttgg ggaaggtagc ggtgcagtac
     1501 ctcgatttcg gcgttttctt tgtctgccaa gtcctgccgc cccgcgtcag tatagatttt
     1561 cgcgctgtct ttgcgctgtt tgaccatttt ggtcaggatg gcggtgattt tggcatcgtc
     1621 ggcttcggtg cgttcgtcca cttcaaactg tttgacggcg gcgttgataa ggcggatggt
     1681 gccgagggaa acttggtctt tggcgcgcat agccgttttc atgtcttcgg taaggcggat
     1741 tttcaggctc ataatgttct cgctgggatg tcggatggaa acggcgggtt ttgatgccgt
     1801 ctgaaaagca aaacacaccg cagagcaggt cttgcggtgt gtcctcatat cacagggctg
     1861 acctgtaatt tgtacttgaa tgtttagtac attttgggcg gcagttgttg gctgcgcagg
     1921 cgtttttgca ggcgttttac ggctgccgct tttttgcgtt tgcgttcggt agtcggtttt
     1981 tcgtaggctt cgcgggcgcg cagctcggtc agcagaccgg ttttttctac ggcgcgtttg
     2041 aaacggcgca tagcgacttc aaatggttca ttctctttta cgcggattgc aggcatttta
     2101 tttcctttaa taaattcggt tgtttcatct gcccatcata tcggtggaaa gggtagggca
     2161 gacggtgttg aaggtttacc gtatcgctgt gccttgcggc ggcgggatac gcgccgtgac
     2221 ggaaacatca cctcctaacg ggtcggcttg tgccgtgtca agctgcttga aaaagcagga
     2281 aaaacaattt tcggattgtc ttatatttat gattcgccgt caatgccgtt cgggataaaa
     2341 atgccgtctg aaagaccggg cgggtttcag acggcatcgg tacgtcagcg ggcggggaca
     2401 atgcccatac gctgtttgag cgggatgtgc ggcgcgccga agagggcggc gtagtaggcg
     2461 gcattggcca tcactttttt gacatagtcg cgcgtttcgg aaaacgggat ggtttcggca
     2521 tataccgcgc cttcgagggg cgtgtccgcc tgccatcggc gcgccctgcc gggaccggcg
     2581 ttatagcctg cggtggcgag gacttcgttg ttttgcaggc ggcgtttggt gtccgccata
     2641 taccacgtcc ccatacggat attgccgtcg gcggtgtaaa gttgtgcggc atccataccg
     2701 attttgccgg cgatttcgcg cgcggtggca ggcataacct gcatcagccc ctgcgcgcct
     2761 acgcgggatt gcgcgcccat aacgaagcgg ctttcctgac gaatcagccc gtaaacccac
     2821 gccggatcga cattaacatt ttgcgcgtgg cggattaccg tgtctttaaa cggcgaaatg
     2881 tagcgcaagg tgtagttgag tttgcggtcg gtgcgttccg cgctgttgac cgccatatcg
     2941 taaaaaccgt ggtcgaacgc ggtttgcgcg gcggtcagca gcttgtcttc atcgaagccg
     3001 cgtgtggcga aacgccattc cgcctgagcc tgacggcgca ttttcgcatc gccggcggtt
     3061 cggctgtttc ggaacagcac cagcgcgcgc ttaatcgcgc cgtcttccgc catacggagg
     3121 acgctgtttt tgccggcatc gggcacattg ttgcgcgtat cgattttgcg ccccaactct
     3181 tcccccgcca gcaccgcata aaaattcctg cccgtcgctg ccgcctgttt gtaaagtttt
     3241 tccgcctctt gcgtgttgcc cgttgcggcg cgactgcgtg ccagccagta gagccaggtc
     3301 gggctttttt gcagtttttc gggcatatgc gagattacgg aagccagctc gtcccaacgt
     3361 cgggcgcgca aggcggcgcg ggcgtaccac tcgatttggt cgtcggtcag ttggcggcgg
     3421 tcggcaactt tgccgtaata gtccaaggcg gcaggcacat tgaggttttg cgactgataa
     3481 tgccccaata cgccccacgc gaaactgcgt tgttcgaggc ttaaaccgct ttccatttcg
     3541 gacagcaggg cggcggcatt cggcgatttg cgtgcttctt tgccgatgac gttcaacagg
     3601 gcgtattcgc gcgaaccttg tgtaccgccg tcaaacgggc tgcccaatgc ggcggcaagg
     3661 ttgcgtgcgt ctgtggtttg gcggccggcc agcagtccgc gcacgcgcct ccaggcgtcg
     3721 ttgccgtcca acaagccgga tgcggctgcc tgttccaaca gtttggtgca gcccgaaggc
     3781 agtttgcccg tatttttaac cagttcagcg gcacgcgtat agtcgttgcg gctcgaatcg
     3841 gcgtagcatt cgacttcttg ggcgcgccct gccggttcga gtttggcgta ttcctgtgca
     3901 aacagcgtcc actgtctgcg tgcgcccaaa gacttcagcc actcgttgcg gacattttcc
     3961 gccatcgcgc tgtcgccggc gttttccaaa taggcggcga cggcggcatc gtttttctgt
     4021 ttcactgcat ccagtgcgga cgggtagccg ccgtaatctg ccagcgtttt tccttcgggt
     4081 tcggcagggc gggtgggaac gcttgccgaa aggtcggcag tttctatatt gtctgccggg
     4141 gtcttgccgg ctggcagtgt gtttgtcgaa gaacacgcgg caagcactag ggccgccagc
     4201 agcggcaggg aatgcttcat agagggtagg tacattggat ttccttaaga atcggaaccc
     4261 tgaacggtca gggttggaaa agacaaaatg ccgtctgaac aggcgtttgc atgaattata
     4321 tgccgaaact gcaccgcctt tggaatgttt ctgacataat ttatattttt caatcagttg
     4381 ccgttttggt gagaaccgct gcctttgccc gtttcagacg gcattgtccg aaatggttgc
     4441 ccgcttcctg ccttattgac aaaaaaacgc tttcccgata atatcctacg aaaattaacc
     4501 tgccgatttg acacagcttg cgggcataac agctaaagcg ttccgacaat ttcagcttta
     4561 tcttccgcgc ccgttgtgtc cgacatcggg ctttgttgta tgggaaagac aatgattatt
     4621 ttggacaagg tttccaagca ttaccaaacg cgcgacaaga cccgttttgc cgccgtcgag
     4681 ccgaccagcc tcgaaatccg cgacggcgaa atcttcgggc tgatgggtta ttcgggtgca
     4741 ggcaaatcca ccctgttgcg cctgattaac ctgttggaac gtcccgacag cggcaaggtc
     4801 aacgtctgcg gacaagagct gaccgcgctc gatgccgccg cattgcgtca ggctcggcag
     4861 aatatcggca tggtgtttca gcagttcaac ctgttgaaca accgcaccgt cgccggcaac
     4921 gtcgccttcc cattggaaat cgccggatgg ccgtctgaaa aaatcaaagc gcgcgttgcc
     4981 gaatgtctcg aaatcgtcgg actgaccgaa cgcgccggcc actatcccgc ccagctttcc
     5041 ggcgggcaga aacaacgtgt cggcatcgcc cgcgcgctcg catccaagcc gcaagtcatc
     5101 ctcgccgacg aacccacttc cgccctcgat cccgccacca cgcgcagcgt cttggaatgt
     5161 ttggaagaca tcaacaaacg cttcaccgta accatcgtca tcgtaaccca cgaaatgagc
     5221 gtcatcagac gactgtgcga ccgcgccgcc ctcttggaca aaggcaaagt cgtcgaaatc
     5281 gtcgaagtac gcggcaacca aatccacgcc caatccgaca tcgggcgcga actgattcgg
     5341 gaggactgat atggcagact taacattcca acaagccgtt tccaacatca tcggtatgaa
     5401 aggcgaaatc gtccgcgcct tgggcgaaac ctttgtgatg gtcgggctgt ccaccacgtt
     5461 tgccgtcatt ttcggcacgc tcttgggcgt attgctcttc gtaacttcca gccgccagct
     5521 gcattacaac aaaccggtaa atttcctgct cgacaactta gtcaacctga tgcgcgcctt
     5581 ccccttcgtc attttgatga tt
//
LOCUS       DAWHRA010000128         5537 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWHRA010000128 DAWHRA010000000
VERSION     DAWHRA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.128"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(34..147)
                     /gene="rrf"
                     /locus_tag="WIB30_001757"
     rRNA            complement(34..147)
                     /gene="rrf"
                     /locus_tag="WIB30_001757"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(244..3135)
                     /locus_tag="WIB30_001758"
     rRNA            complement(244..3135)
                     /locus_tag="WIB30_001758"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3541..3616)
                     /locus_tag="WIB30_001759"
     tRNA            complement(3541..3616)
                     /locus_tag="WIB30_001759"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3581..3583),aa:Ala,seq:tgc)
     gene            complement(3622..3698)
                     /locus_tag="WIB30_001760"
     tRNA            complement(3622..3698)
                     /locus_tag="WIB30_001760"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3662..3664),aa:Ile,seq:gat)
     gene            complement(3800..5340)
                     /locus_tag="WIB30_001761"
     rRNA            complement(3800..5340)
                     /locus_tag="WIB30_001761"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1192 a         1614 c         1183 g         1548 t
ORIGIN      
        1 acccccgaat aatcgggggt tttctgaatg ggtgtttggc agtgacctac tttcgcatgg
       61 aagaaccaca ctatcatcgg cgctgagtcg tttcacggtc ctgttcggga tgggaaggcg
      121 tgggaccaac tcgctatggc cgccaaactt aaactgttac aaatcggtaa agccttaatc
      181 aatatattcg gtaatgactg aatcagtcag taagctttta tctcttgaag ttcttcaaat
      241 gatagagtca agcctcacga gcaattagta tgggttagct tcacgcgtta ccgcgcttcc
      301 acaccccacc tatcaacgtc ctggtctcga acgactcttt agtgcggtta aaccgcaagg
      361 gaagtctcat cttcaggcga gtttcgcgct tagatgcttt cagcgcttat ctcttccgaa
      421 cttagctacc cggctatgca actggcgtta caaccggtac accagaggtt cgtccactcc
      481 ggtcctctcg tactaggagc agcccccgtc aaacttccaa cgcccactgc agatagggac
      541 caaactgtct cacgacgttt taaacccagc tcacgtacca ctttaaatgg cgaacagcca
      601 tacccttggg accgactaca gccccaggat gtgatgagcc gacatcgagg tgccaaactc
      661 cgccgtcgat atgaactctt gggcggaatc agcctgttat ccccggagta ccttttatcc
      721 gttgagcgat ggcccttcca tacagaacca ccggatcact atgtcctgct ttcgcacctg
      781 ctcgacttgt cggtctcgca gttaagctac cttttgccat tgcactatca gtccgatttc
      841 cgaccggact taggtaacct tcgaactcct ccgttacgct ttgggaggag accgccccag
      901 tcaaactgcc taccatgcac ggtccccgac ccggatgacg ggtctgggtt agaacctcaa
      961 agacaccagg gtggtatttc aaggacggct ccacagagac tggcgtctct gcttctaagc
     1021 ctcccaccta tcctacacaa gtgacttcaa agtccaatgc aaagctacag taaaggttca
     1081 cggggtcttt ccgtctagca gcgggtagat tgcatcttca caaccacttc aacttcgctg
     1141 agtctcagga ggagacagtg tggccatcgt tacgccattc gtgcgggtcg gaacttaccc
     1201 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ctggggcttc
     1261 gatccgatgc tctcacatct tcaattaacc ttccagcacc gggcaggcgt cacaccctat
     1321 acgtccactt tcgtgttagc agagtgctgt gtttttaata aacagtcgca gccacctatt
     1381 ctctgcgacc ctcctgggct tacggagcaa gtccttaacc ttagagggca taccttctcc
     1441 cgaagttacg gtatcaattt gccgagttcc ttctcctgag ttctctcaag cgccttagaa
     1501 ttctcatcct gcccacctgt gtcggtttgc ggtacggttc gattcaaact gaagcttagt
     1561 ggcttttcct ggaagcgtgg tatcggttac ttcgtgtccg tagacactcg tcgtcacttc
     1621 tcggtgttaa gaagacccgg atttgcctaa gtcttccacc taccggctta aacaagctat
     1681 tccaacagct tgccaaccta accttctccg tccccacatc gcatttgaat caagtacagg
     1741 aatattaacc tgtttcccat cgactacgca tttctgcctc gccttagggg ccgactcacc
     1801 ctacgccgat gaacgttgcg caggaaacct tgggctttcg gcgagcgggc ttttcacccg
     1861 ctttatcgct actcatgtca acattcgcac ttctgatacc tccagcacac tttacaatgc
     1921 accttcatcg gcctacagaa cgctccccta ccatgccggt aaaccggcat ccgcagcttc
     1981 ggttatagat ttgagccccg ttacatcttc cgcgcaggac gactcgacca gtgagctatt
     2041 acgctttctt taaatgatgg ctgcttctaa gccaacatcc tggctgtctg ggccttccca
     2101 cttcgtttac cacttaatct atcattgggg accttagctg gcggtctggg ttgtttccct
     2161 cttgacaacg gacgttagca cccgctgtct gtctcccgag gaaccacttg atggtattct
     2221 gagtttgcca tgggttggta agttgcaata accccctagc cataacagtg ctttaccccc
     2281 atcagtgtct tgctcgaggc actacctaaa tagttttcgg ggagaaccag ctatctccga
     2341 gtttgtttag cctttcaccc ctatccacag ctcatccccg cattttgcaa catgcgtggg
     2401 ttcggtcctc cagtacctgt tacggcacct tcaacctggc catggataga tcactcggtt
     2461 tcgggtctac acccagcaac tcatcgccct attaagactc ggtttcccta cgcctcccct
     2521 attcggttaa gctcgctact gaatgtaagt cgttgaccca ttatacaaaa ggtacgcagt
     2581 cacaccacta gggcgctccc actgtttgta tgcatcaggt ttcaggttct gtttcactcc
     2641 cctcccgggg ttcttttcgc ctttccctca cggtactggt tcactatcgg tcgatgatga
     2701 gtatttagcc ttggaggatg gtccccccat attcagacag gatttcacgt gtcccgccct
     2761 acttttcgta cgcttagtac cgctgttgag atttcgaata cgggactgtc acccactatg
     2821 gtcaagcttc ccagcttgtt cttctatctc gacagttatt acgtacaggc tcctccgcgt
     2881 tcgctcgcca ctacttgcgg aatctcggtt gatttctttt cctccgggta cttagatggt
     2941 tcagttctcc gggttcgctt ctctaagtct atgtattcaa cttaggatac tgcacagaat
     3001 gcagtgggtt tccccattcg gacatcgcgg gatcattgct ttattgccag ctcccccgcg
     3061 cttttcgcag gcttacacgt ccttcgtcgc ctatcatcgc caaggcatcc acctgatgca
     3121 cttattcact tgactctatc atttcaagaa cttctttgac tttgcctaac attccgttga
     3181 ctagaacatc agacttgaat ttcctacttt gataaagctt actgctttgt tgtgtcttaa
     3241 tcctgccttt tgtgtttcag gattaagtcg atacaatcat cacccaaata ctgtgtttgt
     3301 tttcttttct ctcgcgagag atttttatcc tttgcaaaga ataaaaaatc aaaacaaacg
     3361 ctttgtcttt gtttgttgat ttcggctttc caatttgtta aagatcgatg cgttcgatat
     3421 tgctatctac tgtgcaaatc aaaacgagct gattattata tcagcatttt gttcttggtc
     3481 aagtgtgacg tcgccctgaa tggattctgt tccattcttc cgttttgatt tgtacagtat
     3541 tggtggaggc aaacgggatc gaaccgatga ccccctgctt gcaaagcagg tgctctacca
     3601 actgagctat gcccccgttc ttggtgggtc tgggaggact tgaacctccg accccacgct
     3661 tatcaagcgt gtgctctaac cagctgagct acaaacccgg attctcttct taagcgaatc
     3721 ttgccttcac tcaagcttct tccgcatctt tttcagttta ccgataagtg tgaatgccta
     3781 aagcctcttc tttctctaga aaggaggtga tccagccgca ggttccccta cggctacctt
     3841 gttacgactt caccccagtc atgaagcata ccgtggtaag cggactcctt gcggttatcc
     3901 tacctacttc tggtatcccc cactcccatg gtgtgacggg cggtgtgtac aagacccggg
     3961 aacgtattca ccgcagtatg ctgacctgcg attactagcg attccgactt catgcactcg
     4021 agttgcagag tgcaatccgg actacgatcg gttttgtgag attggctccg cctcgcggct
     4081 tggctaccct ctgtaccgac cattgtatga cgtgtgaagc cctggtcata agggccatga
     4141 ggacttgacg tcatccccac cttcctccgg cttgtcaccg gcagtctcat tagagtgccc
     4201 aactgaatga tggcaactaa tgacaagggt tgcgctcgtt gcgggactta acccaacatc
     4261 tcacgacacg agctgacgac agccatgcag cacctgtgtt acggctcccg aaggcactcc
     4321 tccgtctccg gaggattccg tacatgtcaa gaccaggtaa ggttcttcgc gttgcatcga
     4381 attaatccac atcatccacc gcttgtgcgg gtccccgtca attcctttga gttttaatct
     4441 tgcgaccgta ctccccaggc ggtcaatttc acgcgttagc tacgctacta agcaatcaag
     4501 ttgcccaaca gctaattgac atcgtttagg gcgtggacta ccagggtatc taatcctgtt
     4561 tgctacccac gctttcgggc atgaacgtca gtgttatccc aggaggctgc cttcgccatc
     4621 ggtattcctc cacatctcta cgcatttcac tgctacacgt ggaattctac ctccctctga
     4681 cacactcgag tcacccagtt cagaacgcag ttcccgggtt gagcccgggg atttcacatc
     4741 ctgcttaagt aaccgtctgc gcccgcttta cgcccagtaa ttccgattaa cgctcgcacc
     4801 ctacgtatta ccgcggctgc tggcacgtag ttagccggtg cttattcttc aggtaccgtc
     4861 atcagccgct gatattagca acagcctttt cttccctgac aaaagtcctt tacaacccga
     4921 aggccttctt cagacacgcg gcatggctgg atcaggcttg cgcccattgt ccaaaattcc
     4981 ccactgctgc ctcccgtagg agtctgggcc gtgtctcagt cccagtgtgg cggatcatcc
     5041 tctcagaccc gctactgatc gtcgccttgg taggccttta ccccaccaac cagctaatca
     5101 gatatcggcc gctcgaatag cgcaaggccc aaaggtcccc tgctttctct ctcaagacgt
     5161 atgcggtatt agctgatctt tcgatcagtt atcccccact actcggtacg ttccgatatg
     5221 ttactcaccc gttcgccact cgccacccga gaagcaagct tctctgtgct gccgtccgac
     5281 ttgcatgtgt aaagcatgcc gccagcgttc aatctgagcc aggatcaaac tcttatgttc
     5341 aatctctaac tttttaactt ctggtctgct tcaaagaaac caacaggaca atgttcaaaa
     5401 cattatcttg tctgtctttc aaacagtgtg aggctcaagg cactcacact tatcggtaat
     5461 ctgttttgtt aaagagcgtt tgaattataa agtatttctt ccgcctgtca agatatctct
     5521 cgatatcccc aacattc
//
LOCUS       DAWHRA010000129         5339 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWHRA010000129 DAWHRA010000000
VERSION     DAWHRA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.129"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..898
                     /gene="argF"
                     /locus_tag="WIB30_001762"
     CDS             <1..898
                     /gene="argF"
                     /locus_tag="WIB30_001762"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ3797789.1"
                     /translation="KAGREIQRMKGKNIALIFEKTSTRTRCAFEVAARDQGAGVTYLE
                     PSASQIGHKESIKDTARVLGRMYDAIEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPT
                     QMLADALTMREHSGKPLNQTAFAYVGDARYNMGNSLLILGAKLGMDVRIGAPQSLWPS
                     EGIIAAAHAAAKETGAKITLTENAHEAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKD
                     YRVTPELMAASGNPQVKFMHCLPAFHNRETKVGEWIYETFGLNGVEVTEEIFESPASI
                     VFDQAENRMHTIKAVMVAALGD"
     gene            complement(967..1068)
                     /locus_tag="WIB30_001763"
                     /pseudo
     CDS             complement(967..1068)
                     /locus_tag="WIB30_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1122..2135)
                     /gene="ilvC"
                     /locus_tag="WIB30_001764"
     CDS             complement(1122..2135)
                     /gene="ilvC"
                     /locus_tag="WIB30_001764"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ3797790.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGYVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(2215..2508)
                     /locus_tag="WIB30_001765"
     CDS             complement(2215..2508)
                     /locus_tag="WIB30_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ3797791.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            complement(2575..3066)
                     /gene="ilvN"
                     /locus_tag="WIB30_001766"
     CDS             complement(2575..3066)
                     /gene="ilvN"
                     /locus_tag="WIB30_001766"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ3797792.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(3077..4804)
                     /gene="ilvB"
                     /locus_tag="WIB30_001767"
     CDS             complement(3077..4804)
                     /gene="ilvB"
                     /locus_tag="WIB30_001767"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ3797793.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSSDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSQDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
BASE COUNT         1339 a         1355 c         1307 g         1338 t
ORIGIN      
        1 aaaagcaggg cgcgagattc agcggatgaa agggaaaaac atcgccctga tttttgaaaa
       61 aacctctact cggacgcgct gcgcgtttga agtcgccgcg cgcgatcaag gcgcgggagt
      121 gacttattta gagccgtccg ccagccaaat cgggcataag gaaagcatca aagacaccgc
      181 ccgcgtgttg ggcaggatgt acgatgccat cgaatatcgc ggtttcggtc aggaagttgt
      241 tgaagaattg gcgaaatacg cgggcgtacc cgtgttcaac gggctgacca acgagttcca
      301 tcccacacaa atgcttgccg acgcgctgac tatgcgcgaa cacagcggca aacctttgaa
      361 ccaaaccgcg tttgcctacg tcggcgacgc gcgttacaac atgggcaatt ccctgctgat
      421 tttaggggca aaattgggga tggacgtgcg tatcggcgca ccgcaaagcc tgtggccgtc
      481 tgaaggcatt attgccgccg cacacgccgc cgccaaagaa accggcgcaa aaattaccct
      541 gaccgaaaac gcgcatgaag ccgtgaagaa tgttgatttt attcataccg atgtgtgggt
      601 cagcatgggc gagccgaaag aagtctggca ggaacgcatc gatttgctga aagattaccg
      661 cgttacgccc gaactgatgg cggcatcggg caatccgcaa gtcaaattca tgcactgcct
      721 gcccgccttc cacaaccgcg aaaccaaagt cggcgaatgg atttacgaaa ccttcgggct
      781 gaacggtgtg gaagttacag aagaaatatt cgaaagcccc gccagcatcg tgttcgatca
      841 ggcggaaaac cgtatgcaca cgattaaagc ggtaatggtc gcggctctgg gcgactgaca
      901 gaactgtgcc tgtttaaatt catccgcaac acagataccg tctgaacacg atgttcagac
      961 ggtatccata tatagtggat taaatttaaa ccagtacggc gttgcctcgc cttgccgtac
     1021 tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataaaaa
     1081 aactgcctac acgatgtgta ggtagtcccg tttgaaaaca atcagttttt gtcttggtca
     1141 accaatttgt tggcagtaat ccaaggcatc atggcacgca gttgtgcgcc gactttttca
     1201 acttggtggt cggcattcag acggcggcgg gcagtcatag acgcatagtt gacattaccc
     1261 tcttggataa acatttttgc gtattcgccg gtttgaatgc gtttcagggc attgcgcatg
     1321 gcttctttgc tggaagcatt gaccacttca gggccggtaa cgtattcgcc gtactccgca
     1381 ttgttggaaa tggagtagtt catattggca ataccgcctt cgaaaatcag gtcaacgatc
     1441 agtttcattt cgtgcagaca ttcgaagtaa gccatttcag gcgcgtaacc ggcttcggtc
     1501 agggtttcaa aacccgcctt gatcaactcg accacgccgc cgcacaatac ggcttgttcg
     1561 ccgaacagat cggtttcggt ttcttcgcgg aaagtggttt caatcacacc gcctttggtg
     1621 ccgccgttgg cagccgcata agacagggcg atgtctttgg ctttgccgga attgtcttgg
     1681 taaacggcaa tcagagaagg cacgccgccg ccgcgtttgt attcactgcg tacggtatgg
     1741 cccggacctt tgggggcaac cataatcacg tccaagtcgg cacgcggaac gatttggttg
     1801 tagtgcacgt tgaagccgtg tgcaaatgcc agcgttgcgc cttctttcaa attggctgta
     1861 acttcggcgt gatagacggc aggcatggtt tcgtcaggca gcagcagcat aacgacatcg
     1921 gcttctttgg tcgcttcagc aacggttttg acgacataac cggctgcttc ggcttttttc
     1981 caagaagaac cttggcgcag accaatcacc acgtttacac ccgaatcttt caggttggcg
     2041 gcatgggcat gaccttgcga accgtaaccg atgatggcaa cggttttgcc tttgattagg
     2101 gacagatcgg catctttatc gtaatagact tgcatttgat ttcctttaag gtaaatggtt
     2161 gtcgaagcct taaaatgttg agcggcttcg gacgggttaa acagagtgtg ccgcttaatc
     2221 ggcaacttca ttcatcaata cgatttccaa cgcttcggtt ttgccgtcga cggactggac
     2281 gaaggcttgg aaatgcgcgc tggcgttatg ttcgtcaata gctgcttgag atttccaatt
     2341 ttccacgaaa acaaaacggt tcggtttgcc gatttcctga tggagatcgt agctgatgtt
     2401 gccctcttct gcacggctgg ctttgaccag ttctttaaac tgtgctgcca gtgtttctgt
     2461 gtattccggt ttgacggtaa ccagtgcgac aattttaatg ttcgacataa atctctcctg
     2521 ccgttcgttt ttcagacgac attcaaatac cgtgccgtct gaaaggttac ggcgttaaat
     2581 tttcaaaata cgctcaccgc gaccgatgcc ggccgcgcct gtgcgtacgg tttccaaaat
     2641 ttgggcgcgt ccgaccgttt ccaaaaagga atccagcttg tctgtcgagc cggtaatttc
     2701 aatcgtatag ctgcggtcgg ttacgtcgat gatgctgccc cggtagattt cggtcaagcg
     2761 taaaaattcg tcgcggtctt tgccggcggc acggactttt accaacatca gttcgcgttc
     2821 gacaaaacgg ctttcattca aatcgaccac tttaatcact tcaatcaatt tattgagttg
     2881 cttggtaatt tgttcgatga cctgctcgtc gccgtgggta acgatggtca tccgtgacag
     2941 ggttttgtct tcggtcggcg caaccgccaa agaatcgata ttgtaatcgc gtgcagagaa
     3001 caaaccgacc acgcggctca tcgcacctga ttcgttttca atcagaacag ataagatatg
     3061 tcgcatttgt ctctccttac gcctttccgt ccgcacgcat atgcggcgga agtaccattt
     3121 cgtccaaacc tttgccgttg ccgaccatgg gcatcacatt ctgtttctgg tcggtcagga
     3181 agtcgataaa caccagcctg tctttttggt tcaatgcttc caacaacgca ccttccacat
     3241 cagacttctt gtccacgcgg ataccgatat ggccgtatgc ctcggcaagt ttgacgaaat
     3301 cgggcaaaga atcgaaatag gtttccgact ctcgtccgcc gtaatatatt tcctgccact
     3361 ggcgtaccat accgagataa ccgttgttca gcgtaatgac gttaaccgga atccgatatt
     3421 ggaaacaggt ggacagctct tggatgttca tctggatcga gccgtcgccg gtgatacaga
     3481 atacgtcttg atccggggcg gcaagttttg caccaatcgc ataaggcaga cccacgccca
     3541 tcgtacccaa accgccggaa ttgagccatt ggcgcggacg ttcgaaggga taatattgag
     3601 ccgcaaacat ttgatgctgc cctacatccg atgtgatgat tgccgaattg ccggtaatct
     3661 cggcaagctt ctgaatcaca tattggggct tgataatttc gctgccgttg tcaaaccaca
     3721 agcaatcttg ggaacgccat tcctctatgg ttttccacca tttgcccaaa gcatcttcag
     3781 acggcacgga ctcttgtttt tgccacagcg caaccatctc ggacaaaacg tttttcacgt
     3841 cgccgacaat cggaatgtcc accttcacgc gtttggcgat gctggaagga tcgacatcga
     3901 tatggataac cttcttcgcc ttctcgaaaa atttggacgg tacggaaacc acacggtcgt
     3961 caaaacgcgc acctacggca agaacgacat ccgcattctg catggcaagg tttgcctcgt
     4021 aagtaccgtg cataccgagc ataccgagga attggcggtc gctggaagga taagcgccca
     4081 agcccatcag cgtacccgtg cacggagcac ccgtcattcg gacaaatcgg gtcagctctt
     4141 cagaagcatt acccaacacc acgccgccgc caaaatagac gactggacgt ttggcagatg
     4201 ccaacatctg cacggccttt ttaatctgac cgatatgccc ttgaacaacc ggttgatacg
     4261 aacggataaa aatgtcttcc tgaggatagc tgaatttcgc catcgcctgc gtaacatctt
     4321 tcgggacatc aaccaccaca ggccccggtc ggccgcttgc ggcaatttgg aacgcctttt
     4381 taatggtttc cgccaactca ttgatatccg taaccaggaa attgtgtttg acgcacggac
     4441 gggtaatacc caccgtatca acttcttgga acgcatccgt accaatcagg gaattgccta
     4501 cctgcccgct gatgaccacc atcggaatcg aatccgtata ggcagtagca ataccggtca
     4561 gtgcattggt aacgcccggg ccggatgtaa ccaatgccac gcccacctta ccgctgacgc
     4621 gcgcatacgc atctgccgcg tgtactgccg cctgctcgtg acgggtcaga atgtgcttga
     4681 atttattgag ttggaaaagg gcatcgtaga tttcgataac cgcaccgccg ggataaccga
     4741 aaacgtactc gacaccttcg gctttgagac tctgcactat gatttgcgcg cctgataact
     4801 gcataacgac ctcttttata cggtttcaaa ccaataggga caaaccgctt tgccacagca
     4861 cctgtaatgc aattccacca agcagcgatt tagggtacgc gcattggggg aacacggcaa
     4921 cagacggatt atccaatcaa ttggaaagga acacagagtt tgtgaaaaag agtagaaacg
     4981 ataacgcaaa ccgacagttc aatcaagaaa aatctttcat cttttaatat tttttgaaag
     5041 cagagaaatt attgattgat tttaaaagaa taaaatcagg agtacctttt ttgaaagatg
     5101 gaaattgttg acagtttgtg taggaggggc agatgtgaaa aacccttctt cgatatcaag
     5161 aattgtaaaa tttacagggt ttcatcccaa taaagactcg ggatattgat tgaacttgat
     5221 tttatttttg atatatcaaa aatattccca accatacttc ctgaaaatgg ctcattgcac
     5281 cggactgtat tggacggcat tgacagaacc aagagggcta acaacgactt aatatattg
//
LOCUS       DAWHRA010000130         5289 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWHRA010000130 DAWHRA010000000
VERSION     DAWHRA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.130"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(226..2979)
                     /gene="tbpA"
                     /locus_tag="WIB30_001768"
     CDS             complement(226..2979)
                     /gene="tbpA"
                     /locus_tag="WIB30_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ3797794.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEAAGRGVQSFNRLVPVEDDNQYAHFVVEEECESRNYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKTVFDENKKQAGSLSGNGKYAGDHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKNCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYYQNAKRAYSLKTPPKNGNGGSNPNPNGDKDKPYWVSIGGGNVVTGQICLF
                     GNNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHR
                     TLSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDLEKSFNKEA
                     GIVFKGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINV
                     LGKIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLG
                     YDQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYY
                     TIKKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLE
                     MKF"
     gene            complement(3066..5153)
                     /locus_tag="WIB30_001769"
     CDS             complement(3066..5153)
                     /locus_tag="WIB30_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ3797795.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPKAQKDQGGYGFAMRFKRRNWYQRANPKEDEIKLSENDWEQTDNGDIKNPS
                     KQKNIINALPGNNGGASLQDSSQENQGISKVTDYHNFQYVWSGFFYKQIKNTIEKNGS
                     SITAARNGPDGYIFYKGKDPSRQLPVLGQVTYKGTWDFLTDVKINQKFIDLGNTSTKP
                     GDRYSAFSGELDYIVNKDSDKKDGHVGLGLTTEITVDFEKKTLNGKLIKNNSVSNNDA
                     NAKYTTQYYSLEAQVTGNRFNGKAMATDKPGNGETKQHPFVSDSSSLSGGFFGPKGEE
                     LGFRFLSDDKKVAVVGSAKTKDNTANGNTAAASGGTGAAASGGAAAMPSENSKLTTVL
                     DAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPEASESGNTNQGTNGGTAFTRKFA
                     HTPKSDEKDTQAGTAENGNPAASNTAGDTNGKTKTYAVEVCCSNLNYLKYGLLTRKTA
                     GNTGEGGNGSPAAAQTGAQSMFLQGERTDENKIPSEQNVVYRGSWYGHIANGTSWSGN
                     ASDKEGGNRAEFTVNFADKKLDGTLTAGERTSPTFTITATIQGNGFEGTAKTGDGGFA
                     LDTKNTVETHKAHISNAKVQGGFYGPKAEEMGGWFAYPGDKQAQPPASESGTSAANSA
                     AVVFGAKRQQLVQ"
BASE COUNT         1098 a         1354 c         1334 g         1503 t
ORIGIN      
        1 gacgggattt gaggtttctg tttttgattt tctgttttcg taggaatgac ggtttagagt
       61 tacccgaaac ctcaaaaaaa cctaaaccga acggactgga ttcccgcctg ctcgggaatg
      121 acggatttca ggattacggt gtatcgggga tgatgggaaa cggtgggaat tgtgtaaaaa
      181 atgccgtctg aagcctttca gacggcattt gcggcgtttg gacgtttaaa acttcatttc
      241 caagctaaat gtgtagttgc gaccgggggc ggcatatcgg ttgtaaacgc cgacattttt
      301 gtgttggttg actgcgccgc cggcagtttg ccgcacattt tcccaagtaa catagcggta
      361 gttgaggagg ttgtacacgc ccgcacggag ggtgaagtgt tttttaatcg tgtaataacc
      421 ggacacatcc acaatatacc aagggcgggt acggcgcgcg gtggcttttg tattgcggct
      481 gttgccgttg agcaaagcac ggctgcccaa caactctgtg atttccttgg ctttggaata
      541 agtcagcata ccgttcacac cccatttgcc ttccggttgg tcatagccca agccgacgac
      601 atagcgcgag ggttggatgg catcaaacag atgtgattga atatcggtgc ggtctgcgcg
      661 ttttttgatg tcgcggacac ggacacgatt ataggcaaat gtagaatacc aaccttcggg
      721 caatttatcc catacgccgt tccaatcgat tttgcccaaa acattgatgc cggtaatccg
      781 cgcgctttgg gcattgaggt aagccgggtc gcctttggct gtttctttgc cgttttgaat
      841 ttgcgcttca taaccccgga caatcaaatc gcggtaggca ttgttgaacc aacttgcctc
      901 caagttgccg aaatcgcctt taaacacgat gccggcttct ttgttgaacg atttttccag
      961 atcgattttg accgccttgc tttgaacacc cgcccgccag ccgtacattt ccgcaaacga
     1021 gggcaggcgg aagccggttg aagtgcggta agtcaaatcc agccagtcgg caggtttgag
     1081 gacgatgcct gcgttccagg acagggtgcg gtgcgtgccg gtggaaacgc tgccgtcgtc
     1141 cgaatgcgtg ctgcggtagt cgtagcgcaa gcccgcgccg acatccgccc acctgcccaa
     1201 acggacattg tcccgaactg ccgcgtaata gcttttgccg ttgatgctgc gcggcgtgca
     1261 gtccgtataa gtattgttgc caaagaggca gatttgcccc gtaaccacat ttcccccgcc
     1321 tatgctgacc caatagggtt tgtccttgtc gccgttgggg ttggggtttg agccgccgtt
     1381 gccgttttta gggggcgttt tcaacgaata ggcgcgtttg gcattttgat aataataatc
     1441 ctgatggcgg agattagagc caaagcggtc aaacccgaga ttcacgctca ggttgtggcg
     1501 gattttggcg gtatcgaagg attttttgaa tgccgcctgc aagagcctgt ggctttcccc
     1561 gtaaatcacg cggtcggatt tgtaatagga aaacggtttg tcggcactcg gacggcaatt
     1621 tttgtccgaa ccgtcggcag aacagtgcgt ctgctgaaaa tgattatcca aaccgatacc
     1681 ctgccggtcg taagagaggc gggcataatc cgcccaagtg tctttatcgg cattggtata
     1741 gacatattcc aaaccgtagc ggcttttggt gtgcgtctcg tcgtaaaaca cgcccgtacc
     1801 gtattccgcg cccaccagcg caccgttttc gccgttggta aacagaccgc cgtatttgtg
     1861 gtcgcccgcg tatttgccgt tgccggacaa agaacccgcc tgttttttat tttcatcaaa
     1921 aaccgtcttg gtcaggaatg ccggaaccgt catatcgcgc gtgtcgaaag tttgttgcgt
     1981 gcgttcgagt atgccgccga tgtagtgccg tttgttttca aaacgaaaac ccgggcggaa
     2041 cagccacgac cggctttcgt atgaaagcgg atcggcgagg aagcggttgg gacccgtgta
     2101 gtctcgggtg gaaaccgttt gacgttcgtc tttgccgaca acatcttttt tcggattcgc
     2161 tttacacgtt tcgtaatttc tgctttcgca ttcttcttcg accacgaaat gagcgtattg
     2221 gttgtcgtct tcaaccggca ccagcctgtt aaagctctga acgccgcgtc cggcggcttc
     2281 gtgggcgcgg atttcccccg cgcgccggcc ggtgtggatc agcaaagcct ccgcaccgcc
     2341 gatgcgcccc gccagcgcga tggattgggt aagcccccgg tttttgccgg aataggcggt
     2401 tttactctga atcccccact gcctgccttc cccgataaca tcatcggcgg ttttggtttg
     2461 aaatgcgacc gagcccgcca atgcgccgct gccttgttcg accgagtttg agcctttgct
     2521 gatttcaaca gccttgacgt tttcatactc gatttcattg attgcgccgc tgctgcccgc
     2581 cgtcctcgtc ccgcccaatg ccgcctgagc ggtgtaggac tgtatttgcg ccaagccgtc
     2641 taccgtcaag gaaacgcggt ttttgtccat accgcgtatc gagtagcccg agcttgcgcc
     2701 gcgcccctgt tcgacgacgg cgatgccggg gtcgtaacgc gtcaggtcgc ggatatcgag
     2761 tacctgttcc ttgctgaggg tgtcggcggt tttgaccaat ttgcccaaac cggttacttc
     2821 gttatcgcgg cgggttttct gttttttggc ttttacctgt atggtatcca actgtttttc
     2881 ctgtgcttgt ccggcttgca cattttctgc ataaacgggc agcgcagtca ttaaagacag
     2941 gcataaaata tttaatcgga acaaatgttg ctgttgcata gtgtttccct actcttcgct
     3001 ttcagacggc atcggaagga gcgatgccgt ctgaagcctt attctcgatt gttcggcagc
     3061 cgtgcttatt gcacaagctg ttggcgtttc gcaccgaata ccactgccgc gctgttggcg
     3121 gctgacgtac ctgattccga agcgggcggc tgcgcctgct tgtcgcccgg ataggcaaac
     3181 catccgccca tttcttcggc tttgggcccg taaaaaccgc cctgcacctt ggcgtttgaa
     3241 atatgcgcct tatgtgtctc aacggtattt ttcgtatcca gcgcaaagcc gccatcaccg
     3301 gttttcgccg taccttcaaa gccgttgccc tgaatcgtgg cggtaatggt aaacgtggga
     3361 gacgtacgct ccccagccgt cagcgttccg tcgagttttt tatcggcaaa attcacagta
     3421 aattccgccc tgttgccgcc ctctttatct gaagcattgc cgctccagct tgtgccgttg
     3481 gcaatatgcc cgtaccaaga cccccgataa acgacgtttt gctcgcttgg aatcttgttt
     3541 tcatcggtgc gctcgccttg aaggaacata ctctgcgcgc ccgtttgggc ggcggctggg
     3601 ctgccgttgc cgccttcccc cgtattgccg gcagttttgc gcgtcagcaa cccgtatttc
     3661 agataattga ggttggaaca gcagacttcg actgcatagg tttttgtttt gccattggta
     3721 tcacctgccg tatttgaagc ggctggattg ccattctccg ccgtacctgc ttgggtgtct
     3781 ttttcatcgc ttttcggcgt gtgggcaaat ttgcgggtaa aggctgttcc gccgtttgta
     3841 ccttgattgg tattcccact ttcggaagcc tcgggcagga gcggaatcat aatgccgtcg
     3901 acaaccagtt gggcggcgtt gctgaagttg tcgagatttt tgattgctgt gccgccgtgc
     3961 gtcagctcga ccgcatccaa aaccgtggtc agcttactgt tttcagacgg catagccgcc
     4021 gcaccgcccg atgctgccgc acctgtgccg cctgaagccg ccgcagtatt gccatttgcg
     4081 gtgttgtctt tggttttcgc gctgccgaca acggcaactt ttttatcgtc gctcaaaaag
     4141 cggaaaccca attcctcacc tttcggcccg aaaaagccgc cgctcaaaga agacgagtcg
     4201 gaaacaaagg gatgttgttt ggtttcgccg ttgccgggtt tgtcggttgc catcgccttg
     4261 ccgttgaagc ggttgcctgt tacttgagcc tcaaggctgt aatattgggt ggtgtattta
     4321 gcgttagcat cattattgct tacactgttg tttttaatta attttccgtt gagggttttt
     4381 ttctcaaaat caaccgttat ttccgttgtt aatcccaaac ctacgtgccc gtctttctta
     4441 tcgctatctt tattgacgat ataatccaac tccccggaaa aagcactata tcggtcgccg
     4501 ggtttcgtag aagtattccc taaatctata aatttctgat ttattttcac atcagttaag
     4561 aaatcccaag tccctttata cgtaacctgt cccaatacag ggagttgtct cgagggatct
     4621 ttgcctttat aaaaaatata accgtcagga ccgtttctgg ctgcggttat agatgagccg
     4681 tttttttcaa ttgtattttt aatctgttta taaaaaaaac ccgaccatac gtattggaaa
     4741 ttgtgatagt ccgtaacctt agatataccc tgattttctt gactggaatc ttgcaatgat
     4801 gctcctccat tatttccagg taaggcatta ataatatttt tttgtttgga agggtttttg
     4861 atatcaccat tatctgtttg ttcccaatca ttttcagaga gttttatctc atcttcttta
     4921 ggattcgccc tctgatacca attcctccgc ttgaagcgca ttgcaaaacc gtatccgcct
     4981 tggtcttttt gggctttcgg cttttcggaa gaaacatctt gatattttgg cgcgggacgc
     5041 ggggcttcgg tatcgacaga atcaagatcg aaactgccgc ctccgcccaa acaagcactc
     5101 aacaaaaaca caggcagcac catagcagcc tgatttacca atggattgtt catataaacc
     5161 caattcaatt aaagaatgat aaggattatt attttattta tttttaacaa atttgcaaat
     5221 acttttttat ttttttaggg aatacaccaa aatcccgtca ttcccacgaa agtgggaatc
     5281 tagtttttt
//
LOCUS       DAWHRA010000131         5282 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWHRA010000131 DAWHRA010000000
VERSION     DAWHRA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.131"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(435..2729)
                     /locus_tag="WIB30_001770"
     CDS             complement(435..2729)
                     /locus_tag="WIB30_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ3797796.1"
                     /translation="MRREAKMEQTTLKPIVLSILLTNTPLLAQAHETEQSVDLETVSV
                     VGKSRPRATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPV
                     IRGQTGRRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAG
                     LVDVADGKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKS
                     GDYAVPRYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYD
                     DCHADIIWQKSLINKRYLQLYPHLLTEEDVDYDNPGLSCGFHDDDDAHAHAHNGKPWI
                     DLRNKRYELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNQTQNARIELR
                     HQPIGRLKGSWGVQYLQQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQANWDNFTL
                     EGGVRVEKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLS
                     LTASHQERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLA
                     LYRNRFGNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYR
                     IGVSGDYVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRI
                     DANLDYYRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVY
                     AHSSFLSDTPQMGRSFTGGVNVKF"
     gene            3133..3723
                     /locus_tag="WIB30_001771"
     CDS             3133..3723
                     /locus_tag="WIB30_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ3797797.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFRGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLVEFQNFKPQKI"
     gene            3787..4845
                     /gene="trpD"
                     /locus_tag="WIB30_001772"
     CDS             3787..4845
                     /gene="trpD"
                     /locus_tag="WIB30_001772"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ3797798.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4997..5212)
                     /locus_tag="WIB30_001773"
                     /pseudo
     CDS             complement(4997..5212)
                     /locus_tag="WIB30_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1225 a         1208 c         1490 g         1359 t
ORIGIN      
        1 tgggaatcta gaacccaaat gctaaggcga tttatcggaa acgactgaaa ccgaatgaat
       61 tggattcccg cctgcgcggg aatgacgact tattagttac ctaaaactta aaaaacagaa
      121 acctttacgc cgtcattccc acgaaagtgg gaatccggga acttaacgtt acagtgattt
      181 atcggaaacg gctgaaaccg aacgaattgg attcccgcct gcgcgggaat gacgggatct
      241 tgggtttctg cttttgattt ttctgctttt gcgagaatga cggcgtgaaa gcaagaatga
      301 tgaaacaaag aaaatgggaa tgatggcata gtggtttgtt ctttgtcttt gccatatttc
      361 ctaacaaatt gattaaaaag aaaaaaggtt ttcagaatgc cgtctgaaaa ccttttttgt
      421 ttgcctgtcc gattttaaaa cttcacgttc acgccgccgg taaagctgcg gcccatttgc
      481 ggcgtatcag agagaaagct gctgtgggcg taaacggatt ggttgagcag gttgtcggct
      541 ttgacgtacc aattccactc gccatagcgc gtattgcggc ggtagtttgc gccgaggttg
      601 agcatatggt gtccgggcgt gcgtgtttcg tagcgggcga gtttgttttg ggcgaacacg
      661 cggtagtagt ctaaattggc atcgatgcgg tcggtcagcg aggctttcag gtggacgccg
      721 aggcgcgcag ccggaacgcg aggggcattt tggtcgtcct gtgcgatgaa aggacggttg
      781 ccgtaggcat cttccctgcc gggtagggac ggcaggtttt tcagacggcc tcgtacatag
      841 tcgccggaaa cgccgatgcg gtagcgcggt gtcggtttga agtagatttc gccttctgcg
      901 ccgtagaagt ccgcaccgga ttggttgtag cgcacgagct tcatttcgct gtcgtcttcg
      961 atggatttgg ggccgcgtcc gtcgtttaag gtttgggcgt aaatgtagtt gccgaagcgg
     1021 ttgcggtaga gtgccagatt gtattgccag cggtcgcctt cgtagcccag cgcgagttcg
     1081 atattgttgg aacgctcttt gttgaggtgt ttgttgccga cttcaaaggt gttggtggcg
     1141 acgtgtttgc cgtgtgcgta cagctcttgc gttgacggca ggcgttcctg atgggaggcg
     1201 gtcaggctga gtttgtgttg tggcgtgaaa taccagttgc ccgaaagtgc gaatgagcgg
     1261 gcggtttggc ggtgcgcgcc gaggtcgggc aggggatggt tgtagtagtt ttcccgatca
     1321 atcaatgctt tgtcgtagcg gatggaggct ttttgttttt ccacgcgtac gccgccttca
     1381 agcgtgaagt tgtcccagtt tgcctgttct acaccgaaaa agctgtaatg ttgcacttta
     1441 ttgtcaagca gcatcggttg tttgaccgct tcggatgtgg cagataaagc actggatttt
     1501 tgttgtaaat attgcacgcc ccagctgcct ttcagacggc ctatgggttg gtggcgcaac
     1561 tcgatgcggg cgttttgcgt ttggttgtta aaaaagtttt cgactgcatc gcctgctttt
     1621 tcgtcgtggt ggtagtcgtt gcggttcagg tgtacgcgca gggcttcaaa accggggaat
     1681 ggctgcttcc attcggcgcg gagttcgtag cgtttgttgc gcaggtctat ccaaggtttg
     1741 ccgttgtggg catgggcgtg tgcatcatcg tcgtcgtgga agccgcagct caagcccgga
     1801 ttgtcgtaat cgacgtcttc ttcggtcaac aggtgcggat aaagctgtaa atagcgtttg
     1861 ttaatcaagc tcttttgcca gatgatgtcg gcgtggcaat catcgtattc gtggctgtgg
     1921 gcaggcagac catattggtc gcgacggtcg ctgtacgcta cgccgataaa acctttttcg
     1981 ccaacccaag acagcccgat gctgcccgtt tgcgaatcgg cgtggctgtc gggtaggcgt
     2041 ttcagattgc ggtaacgcgg tacggcgtaa tcgcccgatt tgcggtacaa gccttcggta
     2101 tgcaatacaa agtttttgcc caagccgata ttgatgccgc cggacgtgag tttttccaga
     2161 ttgccgctgc tcaaacgcag ccctagttcg cccgatacgc cgttttcagg cattttttcg
     2221 gggattttgc catcggcaac atccaccagc cccgccacat tgcccgagct gtacaagagc
     2281 gtaaccggcc cgcgcaggat ttcgacctgt tgcgacaagg cggtatctac cataatggcg
     2341 tgatcgggcg aaaaatccgc catatcgcct gtttcgccgt gatggttcaa cactttaatc
     2401 cgcctgcctg tttgaccgcg aataacggga gcggacgcgc cgccgccgta ttgcgaggcg
     2461 tgaatgcccg gcacgccgtc caaagcatcg cccaagttga cggctttttg tcgcaaggtg
     2521 tcgccgctga tgattttgtc ggaggcggtc gaagtgtgca gcagcccgga tgtggcgcgc
     2581 ggacggcttt tgccgacgac gctgaccgtt tccaaatcca ccgattgctc agtttcatgc
     2641 gcttgggcga ggaggggtgt gttggttaaa agaattgata aaacaatggg cttgagagtg
     2701 gtttgttcca ttttggcttc tcgtcgcatt tcaaaagttt gttattatat aacattacat
     2761 tttttatatc ataagatttt gagaacactc agagggcata ggcaaaagtt tttcaaatga
     2821 aacggttgcg gcatcgggcg gtgtccattt gtatccgccg tccttcgggg gcgcggttga
     2881 tgttgacgca gattccgctg tgtttgccca ttatgttcgg ctgcgggata accaaaattt
     2941 atgagtgcat aaaaacggca cgttcccgaa cggtcgaaaa ggtggcaaaa tggcgtactt
     3001 gtcagaacgc gaggctctgc gccaggttca cgagggcgca tcgggcacgc taagatataa
     3061 gagggtatgg atgggggtat cggaaatgca gttaataaca aacaaattat aaatcaatag
     3121 gttaatcaca aaatgctttt gtttatcgac aattacgaca gttttactta caacatcgtc
     3181 cagtatttcg cagaattggg gcaggaagtt gccgtgcgcc gcaacgatga tattacgttg
     3241 gaggaaatcg aggcattgaa tccgcaatat ctcgttatcg gccccggtcc gtgttcgccc
     3301 aaagaagcgg ggatttccgt ggcggcgatg cgccatttcg ccggccggct gccgattatg
     3361 ggcgtgtgcc tcgggcatca gacgataggc gaggcgttcg gcggcaggat agtccgcgcc
     3421 aaaacgctga tgcacggtaa ggtgtcgccc gtgtcccatt cgggcaaggg tatgtttagg
     3481 gggttgccca atccggttac ctgtacgcgt tatcacagcc tcgttatcga tcggaatacg
     3541 atgcccgaat gtttggaagt aacggcttgg actgaggacg gcgagattat gggtgtgcgc
     3601 cataaggaat atgccgtcga gggcgtgcag ttccaccccg aagccctctt gaccgaacac
     3661 ggacatgata tgttaaacaa ttttttagtt gaatttcaaa acttcaaacc gcaaaaaatc
     3721 tgacgtgatg ccgtctgaag cccttcagac ggcattttcg tccgaatatt gaacggagga
     3781 caaaaaatga ttacaccgca acaggccatc gaacgattaa tcagcaataa cgagttgttt
     3841 tacgatgaaa tgaccgactt gatgcgtcag attatgagag gacaggttct gccggagcag
     3901 atagcggcca ttttgacagg attgcgtatc aaggttgaaa ccgtttccga aattaccgca
     3961 gctgcagccg tcatgcgcga gtttgcgaca aaagtgccgc tggagaatgc agaggggctg
     4021 gtcgatatcg tcggtacggg cggggatggc gcgaaaacct tcaatatttc gacgacttcg
     4081 atgtttgttg ccgcagcggc aggcgcgaag gttgccaaac acggaggccg gtcggtctct
     4141 tcctccagcg gtgcggctga cgtggtggag cagatgggcg caaacctcaa cctgactccc
     4201 gaacaggttg cccaaagtat caggcagacc ggcatcgggt ttatgttcgc gcccaatcac
     4261 cacagtgcca tgcgccatgt cgcccctgta cgccgttcgc tcggtttccg aagtattttc
     4321 aacatattgg gtccgttaac gaatcctgcg ggcgcgccga accagctttt gggcgtgttc
     4381 cacaccgatt tgtgcggcat tttgtcgcgg gtcttgcaac aacttggttc aaaacacgtt
     4441 ttggttgttt gcggggaggg cggtttggat gaaattacac tgacgggcaa aacacgcgtt
     4501 gccgagctca aagacggaaa aatcagcgaa tacgacatcc gcccagaaga tttcggtatc
     4561 gaaacccgcc gcaatttgga tgaaatcaaa gttgccaata ctcaggaatc tttgttgaaa
     4621 atgaatgagg tgctggaagg aagagaaggg gctgcgcgcg atatcgtatt gctcaacacc
     4681 gccgccgccc tgtatgccgg aaatgtcgct gcttcgcttt cagacggcat atctgccgca
     4741 cgggaagcca tcgattcagg cagggccaaa tcgaaaaaag aggagtttgt cggttttcaa
     4801 ccacaacaaa gatgccattt tcttggaaag atggagcttg ggtgatgccg ccatgattat
     4861 ggaacttttg tggcaaaaca taagcacttc acgaagagaa cttaccaaac tgtttttata
     4921 taaaaacttg gggctgtact agataaccag accaaattcc cattaactaa ttgtcttaaa
     4981 atctgaattt gagattctat ttaaaatgcc attggcattt ctttaaatgc agccccaaat
     5041 gctctttggg aatgccgtta aacttacgta aatggctaaa ttcactaata tcaagcacat
     5101 cataactacg aaaggtatcc gtgtatacaa tgccatcagg cttaactttc tttcggataa
     5161 ttggcaacaa tgtcgctgat tgcgcattag gaacaacgac ggtataaacc ttatatattg
     5221 cgtccctaag aagggacgat taacaaaaat taacgtcctt tactttctac aagtaacagg
     5281 gc
//
LOCUS       DAWHRA010000132         5259 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWHRA010000132 DAWHRA010000000
VERSION     DAWHRA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.132"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(104..2935)
                     /gene="lbpA"
                     /locus_tag="WIB30_001774"
                     /gene_synonym="tbpA"
     CDS             complement(104..2935)
                     /gene="lbpA"
                     /locus_tag="WIB30_001774"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ3797799.1"
                     /translation="MNRRHTFPLTLTALAVAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDIKRKTREPFFSVEGERESK
                     PVAKLAGYGKYLNNQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGNDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISESTG
                     FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2932..5103)
                     /locus_tag="WIB30_001775"
     CDS             complement(2932..5103)
                     /locus_tag="WIB30_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ3797800.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGPSVETTPVNRPAVGAAMRLPRRNTAFHREDGTVIPDSKQAEEQLSFKEG
                     DVLFLYGSKENKLQQLKSEIHKRDPDVEIRTSENENKKYDYKFVDAGYVYVKGKDEIK
                     WTSDHKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYFGNWQYMTDVKRHRAGKAVGI
                     DNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKILKGELIKNQYVRDRNGP
                     YNPLTIYDITATLDGNRFTGSAKVNTKLKTSHADKEHLFFHDDADQRLEGGFFGDNGE
                     ELAGRFISNDKSVFGVFAGKQKTETANASDTNPALPSGKHTKILDSLKISVDEATDSN
                     ARKFAISSMPDFGHPDKLLVEGREISLVKDTQTIDLADGRKMTVSACCDFLTYVKLGR
                     IKTDRPASKPKAEDENSEDEIGESEENEEDLVAEEENTEDEVVEDEDREEDEVSEDGN
                     SEDEEEIAEEDDDEAEEEEVEETEEESPVEESGGDSGSILPTPEAPKGRDIDLFLKGI
                     RTAEADIPKTGTAHYTGTWEARIGVPDKKDDQLYGTTFIQKDSYANQGAKAQFTVDFG
                     KKSISGTLTEKNDTHPAVYIEKGVIDGNGFHATARTRDNGIDLSGQGSTNPQRFEANN
                     LLVTGGFYGPQAAELGGNIIDSDRKFGAVFGAKKDDKEATR"
BASE COUNT         1133 a         1406 c         1224 g         1496 t
ORIGIN      
        1 cggaaatctt gtttatatcg aatcaaaaaa acctgcacct taatcagttg gcggtttagt
       61 ccgacttttg gggtgcagat caagctttca gacggtattt cctttaaaac ttcatttcga
      121 gcgcgaggct gaagttcctg cctggtgcgg cataccttcc atagttgctg tcgccgccgt
      181 gccggtttgc cgtgctttcc gcggtctggc gcaaggattc ccaagtaacg tagcggtagt
      241 tgccgatatt gtagatagcc gccctcaagg tcagccgttt tttcagattc agataggcgg
      301 aaacgtctgc cgtcgaccaa gaagacgacg ctctttttgt cgaatatcgt ttttgatcgc
      361 ctgccagata agcaagctcg tcagggtttt tccctttgga ataggtcagc ataatgtttg
      421 cgccccattt cccctctggc tggtcgtatc cgaaccccaa aacataacgc gacggctgta
      481 ccgcatccaa agcatagctg cggagggaca gtcccggccg gttggatacc gatttcggtt
      541 tgatgcggtt gtacgccaaa gtcgtgtaca aaccttcggg cagtttgcca tacacgccgt
      601 tccagtcgat ttttcccaat atattaacgc cttgaagcga catattttgg gcattgtaat
      661 aatcgcgtat atcaatctct gtcaattgtc ctgcctgatt cggcaatttg gttttgtgat
      721 cggcaacggc aatcatatcg gtataacggt tgcggaagct gctgatttcc aaaaagccga
      781 aatcgccctt ccactgcaaa ccgatttcgc ggttggctgc cttttccgat ttcagggcgg
      841 gacgctgcca gcctttcgga taatcgtgat aaatgtctat cccgaaaagt tcttggaatg
      901 agggcgttct gaagccgctg gaggcgcggt aagacaaaga aaaatgccgg ttcggtttga
      961 acacgatgcc gctgttccac gaacggtcaa cataccgccc gctgcggacg agttcttccg
     1021 acgtggtgaa gtttttccgg tcatacctgc cacccaagct gaaatcaaaa tatttgccga
     1081 ttgaaaaacg gtcgttcaaa gaaatatgga tattgctgcc gttgattttt cttggcacgc
     1141 atttgcggga acgcagggtt tcgatgtagc cgcagaccga cccttcgacg acttcgggct
     1201 tacccaaacg gtacttatct tgattctttt catcgaatcc cgtggattcc gaaatccttg
     1261 ccgcattgtg ggaaagctgt tcagggcggg agatcgcgtt ggaagcatcg taaccgaagc
     1321 ccaaagtcag atggtgtttc gtccatttgt ttttcagcga cttctcaaac gaggcattca
     1381 aaacattgtg ctgttcgcgg tagtggaaac ggtcgctgct gtcgtaggaa tacggtttgt
     1441 ccgccgacgc gcggcaggat ttgtccacag caggatacac ggcacaattc agcttcagcg
     1501 tgttgttgtc ggttgccacg ccctgtttgt caaacgacaa caccgccttg tccgcccaat
     1561 tgtcagaata cgcttcgttt tcataacggt acagcaaacc catacggcgg cggcggtgat
     1621 gttcgtcaat aaatttggtg cgggaatatt tcaaacctat gcccctgacc aactttttat
     1681 cgcccttcca ctcttctata ttcggcacaa aatacaagcc gtcgcggaaa tcgttgccgt
     1741 cgtacacccc gctcttgtct ctaaactttt ccgcctcgtc cgtaccgtaa tactgttttt
     1801 ccgtcatatc gcggatgtcg taacgctgtt tggtatcctc aaacacaccg ccgacataat
     1861 gcctgccgcc gaagcggtag cccagcttgg caagccacga accgctgcgg taatccatcg
     1921 gatcgggcaa tatcctgccg ccgcccgtgt aagcttgggc ggacagattt tcgtggcgcg
     1981 cctgcgcctc ccgcacctgc gcctcttctt cagcacttaa aggctgattt tgttcaatac
     2041 gttcttttac ccagcggttg agctggttgt tcaaatattt cccgtagccc gccaattttg
     2101 ccacgggctt ggattcacgc tcgccctcta ctgagaaaaa tggctctctt gtcttgcgtt
     2161 taatatcgta tgtctggcgg aacgcgtcca aacggtttat gccgtatgcc accccgtccg
     2221 caatatcgcc gtgcgggcgc gtttcccgcc cttggcgttc ggtacggatt agcagccctt
     2281 cccaaccgtc tttgctgaac cccgcgccga gcgacttcat aaattggcgg tttttactgc
     2341 cgtaggcggt ttttgcctgt atcccccagc ttttgccgtc tgaaatcagg tctgccgcct
     2401 ctttggtgcg gaaggcgacc gcgccgccga gtgcgccgct gccgtgatcg gacgaaccgg
     2461 cacctttgtc gatttccacc gtgctgatgt tttcatattc gatttcgttg attgcaccgc
     2521 tgccgccgcg tccgccgtat ccgctcaacg atccctgcac ggtaaacgcc tgtatttggg
     2581 caacgccgtc aaccgaaacc gccacacggt ttttatccac gccgcgtatc gagtagccgc
     2641 cgctcgcgcc gttgccctgt tcgacaaccg ccacgcccgg atcgtagcgc gtcaggtcgc
     2701 ggataccgag tacctgttct ttgttcaacg tttccgacgt tttgacgatt ttgcccaaac
     2761 cggtcgcctc tttcgatcgc cgtcccactt tggcggcacg gacggtaacc tctttcaggg
     2821 attgggtctg cgccgcatca ggtgccgccg tttcagggtt tgccgcatat gacgggaagg
     2881 cggcggcaac agccaaggca gtcagggtaa gtgggaatgt atgccttctg ttcatcgtgt
     2941 tgcctccttg tcatctttct tcgcaccgaa taccgcgccg aatttccggt cgctgtcgat
     3001 aatattaccg cccaattccg ccgcctgcgg tccataaaag ccgcctgtta caagaagatt
     3061 gttggcttcg aagcgctgcg ggttggtcga accctgcccg gaaaggtcaa taccgttatc
     3121 ccgagtgcgt gctgtcgcgt ggaaaccgtt gccatcaatc acaccttttt caatataaac
     3181 agcggggtgt gtatcatttt tttctgtcaa cgttccggaa atcgatttct tgccgaaatc
     3241 aacggtaaat tgtgcttttg ccccttgatt tgcatagcta tccttttgaa tgaacgtagt
     3301 gccatataac tgatcgtctt tcttatccgg tacaccgata cgcgcttccc aagtgccggt
     3361 ataatgcgcc gttccggttt ttggaatgtc ggcttccgcc gtgcggatac ctttcaggaa
     3421 aaggtcgatg tccctgcctt taggggcttc cggagtgggc aggatgctgc ctgaatcgcc
     3481 accgctttct tccaccggcg attcttcttc ggtttcttca acttcctcct cttcggcttc
     3541 atcatcatct tcttcggcga tttcttcttc gtcttcactg ttaccatctt cggaaacttc
     3601 gtcttcttct ctgtcttcat cttctacgac ttcgtcttcc gtgttttctt cttcagcgac
     3661 caaatcctct tcattttcct cgctttcacc aatttcatct tcggaatttt catcttccgc
     3721 ctttggttta cttgccgggc ggtcggtttt tatccgtccg agtttcacat aggtcaaaaa
     3781 gtcgcaacaa gcactgacgg tcattttcct gccgtcggca agatcaatgg tttgagtatc
     3841 tttgaccaag gagatttcac gcccttcgac aagaagcttg tcgggatgac caaaatcggg
     3901 catagaggaa atggcaaact tacgggcatt gctatcagtc gcctcgtcaa cggaaatttt
     3961 tagagaatcc aagattttgg tgtgttttcc agacggcagg gcaggatttg tatctgatgc
     4021 gtttgctgtc tctgtttttt gtttgcctgc gaatacgccg aatacgcttt tgtcgttgct
     4081 gataaaccgt ccggcaagct cttctccgtt atcaccgaaa aaaccgccct caagccgctg
     4141 atcggcatcg tcatggaaaa acaaatgctc tttatcagcg tggctcgtct tcaacttggt
     4201 gttaactttg gcactgccgg taaagcggtt gccgtccaat gttgcagtaa tgtcgtaaat
     4261 ggtcagcgga ttatagggac catttctatc tcgcacatac tgatttttaa tcagctcgcc
     4321 ctttaggatt tttttgtcga aatcaaccgt atatttggca ggatgctttt ccctttcgtc
     4381 gacatcctta gccgcataag aagttgcacc aacatcgtta ccataaaatg tgatataacc
     4441 caaattgtca atgccaaccg ccttacctgc tcgatgacgt ttgacatcgg tcatatattg
     4501 ccagttacca aaatattcca ccgttcccgc actcggtaaa gattgggaag gacgttctcc
     4561 ggaataatat acaaaaccgt cataacctaa gcggttggaa aactgcttgt gatctgaagt
     4621 ccacttaatt tcatcttttc cctttacata tacataacct gcatctacaa atttataatc
     4681 atatttttta ttttcatttt ctgatgtcct aatttctaca tcgggatcac gtttatgaat
     4741 ttcgctttta agttgttgaa gtttattttc ttttgaaccg tataaaaaca gaacatcacc
     4801 ttctttaaac gacagctgtt cttctgcttg tttgctatcg ggaattaccg tgccatcttc
     4861 acgatgaaaa gcagtattcc gccttggcag ccgcattgcc gcaccgacgg cgggccggtt
     4921 gaccggcgtg gtttctaccg aaggcccggc agggggggga gtgggaacgt ccttagactt
     4981 gaaagtgacg gggtacgcgg tcggcgttga ttcgacaaca ggctgcacgc cgaaattgcc
     5041 gccgatacaa gatgctaaaa gtaagggcaa caagacaatg ccgccataat tcggtttaca
     5101 catccctact tttcctccta tttgattaat aataattatc attatattaa tatgtacaga
     5161 taatatcaag ccgtttttat agtgaattaa caaaaatccg gacaaggcga cgagccgcag
     5221 acagtacaga tacattccgt cattcccacg aacctacat
//
LOCUS       DAWHRA010000133         5233 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWHRA010000133 DAWHRA010000000
VERSION     DAWHRA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.133"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            86..1561
                     /locus_tag="WIB30_001776"
     CDS             86..1561
                     /locus_tag="WIB30_001776"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ3797801.1"
                     /translation="MISKQEYQAQAAQDYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSNPESEWQETQNKARAVIHAAQMVQEGLDK"
     gene            1558..2103
                     /locus_tag="WIB30_001777"
     CDS             1558..2103
                     /locus_tag="WIB30_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ3797802.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKNREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            2100..3236
                     /locus_tag="WIB30_001778"
     CDS             2100..3236
                     /locus_tag="WIB30_001778"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ3797803.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPGSDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTIDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGKPDWFPLQS
                     RPDARLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            3283..3765
                     /locus_tag="WIB30_001779"
     CDS             3283..3765
                     /locus_tag="WIB30_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3797804.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            3871..4926
                     /locus_tag="WIB30_001780"
     CDS             3871..4926
                     /locus_tag="WIB30_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3797805.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALLDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISTESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            4923..5216
                     /locus_tag="WIB30_001781"
     CDS             4923..5216
                     /locus_tag="WIB30_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797806.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITGRNGKPDTKPNTAAKM
                     PSEAVGATILIWGPLQGRITFLNHPFDSQINSAFACQKQNQYFAVRIGTFIVD"
BASE COUNT         1494 a         1593 c         1115 g         1031 t
ORIGIN      
        1 cggttagact gtcaacaatt ccgataaaat gctatactcg cgcccattgc cgcgccgtcc
       61 gaaaccgccc tttgggaaag cagaaatgat cagcaaacaa gaataccaag cccaagccgc
      121 ccaagactac aaccgcatcc cgctcgtgca agaacttctt gccgacttgg atacgccgct
      181 ttccctctat ctcaaactcg ccaaccgccc ctatacctac ctgctcgaat ccgttgtcgg
      241 tggcgaacgt ttcggccgct attcctttat cggcctgcct tgcagccact atctcaaagc
      301 cggcggcaaa cacgtcgatg tttatcaaaa cggcgaaatt gtcgaacaac acgacggcaa
      361 tccgctgccc tttatcgaag ccttccacaa ccgcttcaaa acacccgaaa tcccaagcct
      421 gccgcgcttt accggcggac tggtcggcta cttcggttac gaaaccatct acaattttga
      481 acacttcgcc caccgcctga aaaacaccac caaagccgac ccgctcggca cgcccgacat
      541 cttgctgatg ctgtcgcaag aattggcggt gatcgacaat ttgagcggca aaatccatct
      601 cgtcgtttac gccgatccgt cgcagcccga cggctacgaa cgcgcccgcg aacgcctcga
      661 agacatccgc acccagttgc gccaaagctg cgccatcccg ctctcgctcg gcagcaaaca
      721 caccgaagcc gtcagcgaat tcggcgaaga gccgttcaaa gcctgcgtta acaaaatcaa
      781 agactacatc ttcgcaggcg actgcatgca ggttgtcccc agccagcgca tgagcatgga
      841 attcaccgac agcccgctcg ccctctaccg cgccttgcgc acgcttaacc cttcgcctta
      901 tctcttttac tacgatttcg gcgatttcca catcgtcggc tcctcgcccg aaatccttgt
      961 ccgccgcgaa cgcgacgatg tcatcgtccg ccccatcgcc ggcacgcgcc tgcgcggcaa
     1021 aacccccgcc gaagaccttg ccaacgaaca agatttgtta agcgatgcca aagaaatcgc
     1081 cgaacacgtc atgttgattg acttaggacg caacgacgtc ggccgcatca gtaaaaccgg
     1141 cgaagtcaaa gttaccgaca aaatggtgat tgaaaaatac tcccatgtaa tgcacatcgt
     1201 ttccaacgtc gaaggccgtc tgaaagacgg catgaccaac atggacatcc tcgccgccac
     1261 gttccctgcc ggcacacttt ccggcgcacc caaagtccgc gccatggaaa tcatcgaaga
     1321 agtcgaacca agcaaacgcg gcatctacgg cggcgccgtc ggcgtatggg gtttcaacaa
     1381 cgacatggat ttggcaatcg ccatccgcac cgccgtagta aaaaacaaca cgctatacgt
     1441 ccaaagcggc gcaggcgtag ttgccgattc caatcctgaa tcagaatggc aggaaacgca
     1501 gaacaaagcc cgtgccgtca tacacgccgc gcaaatggta caggaagggt tggataaatg
     1561 acttatgaaa aactttatga gcttcgccaa acactcagac cgactaccaa agacggtctc
     1621 tatacagata atgaaaaaaa tcgagaaatc ctaacggtta gatggagcgg caacacagaa
     1681 aatcctaaaa atttctcagc tgtcgctata ggaattaatc caagtaaagc aaatgatgaa
     1741 agaagcgaca aaacccttac ccaacttgca cgtttcttgg atatgtacgg ttttaccaac
     1801 ttcaaaatgt taaatatttt ttccagttac tctacacagc aaacaggaat tagggcaaac
     1861 acccaaaccg atttttccaa atttaaagga tgtttggaag atgctgatat gattattctt
     1921 gcatggggta cagaccgcgg tgcttataag gatgaaaaaa acagaatact ggaatttcta
     1981 aaagcagaaa aatttatgga aaaagtattc tgtatttctg aaactggtaa ttccagcgat
     2041 acaagacatc cgtcaagaat tagttatagc tatcaacttg tacaatttga agagtcagca
     2101 tgaaaaacat atctctttct ccgcccgcca tgcttggcat cctcggcggc ggacaattag
     2161 gcagaatgtt taccgttgcc gccaaaacca tgggctacaa agtaaccgtt ctcgaccccg
     2221 atccggacgc gccggcagca gaatttgccg actgtcattt atgcgcgccg tttaacgacc
     2281 aagctgcttt ggacgaattg gcaaaatgcg cggcggtgac cactgaattt gaaaacgtca
     2341 atgccgatgc gatgcgtttt ctcgccaaac acaccaatgt ttcccccggc agcgactgcg
     2401 ttgccatcgc gcaaaaccgc attcaggaaa aggcatggat acgcaaagca ggattgcaaa
     2461 ccgcgccgta tcaagtggtt tgtaaggctg aagacattac tgaagcaagt gcgcaattct
     2521 tgcccggcat cctgaaaacg gccactttgg gctacgacgg caaaggtcaa atccgcgtca
     2581 aaacggtgga tgaactcaaa gctgcgtttg ccgaacacgg cggcgtggat tgcgttttgg
     2641 aaaaaatggt ggatttgcgc agtgaaattt ccgtaatcgt atgccgtttg aacaatgaca
     2701 acgtgcaaac tttcgaccct gccgaaaaca tccacgaaaa cggcatcttg gcttattcca
     2761 tcgtccccgc gcgactgagt gccgacgtgc agcaacaggc gcggcagatg gcgcaacgct
     2821 tggcggacga attggattat gtcggcgtat tggcggtaga aatgtttgtt gtcggtgaca
     2881 cacatgaatt ggtcgtcaac gaaatcgcgc cgcgtccgca caactccggc caccatacca
     2941 tcgatgcctg cgcggcagac caattccagc aacaggtccg cctgatgtgc aacctgccac
     3001 ctgccgatac caaattgctg agttcttgct gtatggcgaa tattttgggc gacgtttggc
     3061 aggaagacgg cggcaaaccg gattggtttc ccctgcaaag ccggccggac gcgcgcctgc
     3121 acctttacgg caaaaaaacc gcgcacaaag ggcggaaaat gggacacttt accgttttaa
     3181 ccaccgattc ggacaccgca tttcaagaag caaaaaaact gcatcagtcc ctataatccg
     3241 accaacggca cagatgccgt ctgaagccgc cgacatactg ccatgaacct gctgacactg
     3301 ttacgccccg ccaccgtgca agactgcaaa gacatcttca aagtccacct gcactccgtc
     3361 caatacacct gcatcctcag ctacaacgaa cacgccctaa aagtatggga aggcttgtta
     3421 aacacagaaa gctacctgcc gaccatttcc gatcccgaca aagcactttg ggttgccgaa
     3481 tacaaaggca acatacaggg atttttccaa atcgactgcc aagaagcaca actcgatgca
     3541 ctttacgtcc accccctgtt ccacaacctc ggactgggca ctgccctact tcatcaggcg
     3601 gaaaccatcg cccacaaatc cggtttgagc tttctgaaac tctacgcatc gctcaactcc
     3661 gtcccgtttt accttctcaa ccgctacgaa tcgctcggtt cggcagtatt gcagcttgac
     3721 cccagcatca aaatcaaatg cgaactgatg cggaaacacc tctgatccgt ccttataacc
     3781 aaaactgata atcttcaaat caaacattat ttccacaagc gcagacagcc gtataaagtt
     3841 gccccatcta ccaaccgaca ggacaagcat atgaaaacct acgcaccggc actctatacc
     3901 gcagccctgc tcaccgcctg cagccccgca gccgattcaa accatccgtc cggacaaaat
     3961 gccccggcca ataccgaatc cgacggaaaa aacattaccc tgctcaacgc ctcatacgat
     4021 gtggcacggg atttttacaa agaatacaac cccttattta tcaaaacata ccaatccgaa
     4081 caccccggca catccgtcag catccaacag tcccacggcg gctccagcaa acaggcatta
     4141 tccgtagcca acggccttca agccgatgtc gtaaccatga accaatcctc cgacatcgac
     4201 ctgctcgaaa aaaaaggact ggtagaaaaa ggctggcaac aagccctcct cgaccacgcc
     4261 gcgccctaca ccagcactat ggttttcctt gtccgaaaaa acaaccccaa acagatccgc
     4321 gattggaacg accttgccaa agacggcgtt aacatcgtca tcgccaatcc caaaacctcg
     4381 ggcaacggac gctacgcctt cctcggcgca tacggttacg gtctgaaaac caccaacggc
     4441 aacgaacagg aagcccaaaa actcgtcgca tccatcctca aaaacacccc cgtttttgaa
     4501 aacggcggac gcgccgccac caccaccttc acacaacgca acatcggcga cgtactcatc
     4561 acttttgaaa acgaagccaa ctacgtcagc aaaaaactga cccaaggaca gtttgaaatc
     4621 gtctatccga gctacaccat ttccaccgaa agccccgtcg ccgtcgtcaa cagcgtcgtc
     4681 gcgaaaaaag gcacgcaaaa aaccgcccgc gcctatctcg aatacctttg gagcgaaccg
     4741 gcgcaagaac tcgccgcctc actctacctg cgcccccgca accccgaagt attggcaaga
     4801 cacaaagccg acttcccaga cttagactca ttctctcccg aagaaaaatt cggcggatgg
     4861 gacaacatca tgaaaaccta cttcgccgac ggcggcatat tcgaccgcct gaccgcacaa
     4921 aaatgaccgc tatgccgtct gaaccgcctt tcagacggca tttcacacat caaaacagaa
     4981 aaaccgcgcc caaaatcatc atcacaggac gcaacggcaa accggataca aaacccaaca
     5041 ccgccgccaa aatgccgtct gaagccgtcg gcgcaaccat ccttatctgg ggcccgcttc
     5101 aggggcgcat aactttcctt aaccatcctt ttgacagcca aataaacagt gcttttgcct
     5161 gtcagaaaca aaaccaatat tttgcggttc gtatcggaac atttatagtg gattaacaaa
     5221 aaccagtaca gcg
//
LOCUS       DAWHRA010000134         5223 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWHRA010000134 DAWHRA010000000
VERSION     DAWHRA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.134"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            96..1493
                     /gene="aspA"
                     /locus_tag="WIB30_001782"
     CDS             96..1493
                     /gene="aspA"
                     /locus_tag="WIB30_001782"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ3797807.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1565..2440)
                     /locus_tag="WIB30_001783"
     CDS             complement(1565..2440)
                     /locus_tag="WIB30_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3797808.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2442..3164)
                     /locus_tag="WIB30_001784"
     CDS             complement(2442..3164)
                     /locus_tag="WIB30_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ3797809.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3187..3666)
                     /gene="ybaK"
                     /locus_tag="WIB30_001785"
     CDS             complement(3187..3666)
                     /gene="ybaK"
                     /locus_tag="WIB30_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ3797810.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3741..4103
                     /locus_tag="WIB30_001786"
     CDS             3741..4103
                     /locus_tag="WIB30_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ3797811.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            4138..5001
                     /locus_tag="WIB30_001787"
     CDS             4138..5001
                     /locus_tag="WIB30_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ3797812.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPSLAFTAECGSDGICSVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKTLTVPNGGIFKA
                     VHLFFKTENAE"
     gene            5094..>5223
                     /locus_tag="WIB30_001788"
     CDS             5094..>5223
                     /locus_tag="WIB30_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ3797813.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLS"
BASE COUNT         1309 a         1500 c         1276 g         1138 t
ORIGIN      
        1 atttcatccg gccggtatag tgttcgtcct gcggcggcat ctttccgatg gcgcattttt
       61 tcaatattaa ttatccgact tacaaaggta aaaacatgac tgtccgtatc gaacacgatt
      121 tattgggcga ccgcgagatt cccgccgaag tgtattgggg catccacacc ctgcgcgcca
      181 ttgaaaactt taaaatctcc acacaaaaaa tttctgacgt gccgcagttt gtccgcagta
      241 tggtgatggt gaaaaaggcg acggcacagg caaacggtaa gttgggcgcg gtcaagcccg
      301 aaatcgccgc cgccattgaa aaggcttgcg acgaagttct gctgaacaac cgctgcctcg
      361 accaattccc gtccgatgtg tatcagggcg gtgcgggtac ttcggtcaat atgaacacca
      421 acgaagtcat cgccaacctc gcattggaag tcttgggata cgaaaaaggc cgctacgata
      481 tcgtcaatcc gatggatcat gtcaacgcca gccaatccac caacgatgcc taccctaccg
      541 gcttccgcct tgccgtgtat tacagcatcg gcgaattgct cgacaaactg gccgtcctga
      601 aaaacgcctt tgccgccaaa gccgaagagt ttaaagacgt tttgaaaatg ggccgcaccc
      661 agcttcaaga tgccgtgcca atgactacag gacaagaatt ccaatctttc caagtgttgt
      721 tggaagaaga aatcctcaac ctcgaccgca cgcgccaact gctgttggaa gtcaatttgg
      781 gcgcaacggc aatcggcacg ggcgtgaaca cgcccaaagg ctacgccgaa ctggtggtca
      841 aaaaactctc cgaagtcagc ggcttgcctt gcaaactgac cgaaaacctg attgaggcga
      901 cctccgactg cggcgcatat gtgatggtac acggcgcatt gaagcgcacg gcggtcaaac
      961 tctccaaaat ctgcaacgac ttgcgcctgc tctcttccgg cccgcgtgcc ggcttgaaag
     1021 aaatcaacct gcccgaatta caggccggtt cttccattat gcctgccaaa gtcaatcccg
     1081 tgattcccga agtggtcaac caagtctgct tcaaagtcat cggcaacgat acgacgatta
     1141 ccttcgccgc cgaagccggg caactgcaat taaacgtgat ggagccggtc atcgcccaat
     1201 gtatgtttga aaccatttcc ctcttgggca atgcagcaat caacctgtcc gacaaatgcg
     1261 tcaaaggcat tacggtcaac cgcgaaatct gcgaacgtta cgttttcaac tccatcggtt
     1321 tggtcactta tctgaatccg tacatcggcc accacaacgg cgacttggtc ggcaaaatct
     1381 gcgcccagac cggcaaaggc gtgcgcgagg ttgtcttgga acgcgggctg ttgagcgaag
     1441 aagaactcaa ccgcatcctc tctcctgaaa acctgatgaa cccccatctg taacgttgca
     1501 gacaaatgcc gtctgaagca gggatgctgc ttcagacggc attttgttat atcaatcagc
     1561 cggattatat gccgaacagt accttgactt ccccccaata aaacaggcag gcgatagaac
     1621 acagtgccaa cacgattccc gccgtattga ttgtgttgat tttttcctta aagaacaatg
     1681 cgcccgaaag cgtacccaaa acaatcacgc cgatattcat accggcaaaa accaaggtcg
     1741 gattatcctt catcatttgg tgcgcggtga tgtaggttac gatattcata aaattcaaac
     1801 cgcccaagaa tatgccgcca accacactct caacacgcca tctgaccgat ttggcaaaca
     1861 ggcaggcaaa catcagcaca cccgccagca caaatgcaac cagcaggttg cccgcaaatg
     1921 ccgttccgct tttggcaagc tgtttgaaca ggatatcgat aatgccgtaa cctgcccaca
     1981 cgcccagcag caatgccgcc tgccgccacg cgcttcctga ttttttgcca ccgctgtgtt
     2041 tccaaagcag gcagaacagt gcggcaaatg cgaggcacag cccgattagt ctgccttcgc
     2101 tgagtttttc gccaaacagc gtcaaggcgg caacaatcgg caaaaacagc gacaaacgct
     2161 gcgccgcgtc ggatttgacg ataccggcgg attctacgga tttgcccata atgacaaata
     2221 cggacggcag cagcacgccc aatgcggcaa acagccacca tgtcggcaaa aatgcgccga
     2281 tattgccgac atccggcttc aataccagca gggtcagtat gaccgcgacc acataattga
     2341 cggcgaccgc ctgcgcgatg tcgatttttt tcttccttgc cattttcagc aaaatggaaa
     2401 ccgacacgct gaacacgatg ctgattaaca ggtaagccat actatatcca tttctcgggt
     2461 gcaaccgccc tgccgccgtt gaagcggata aagccggcga tgatgcggta gaaataccag
     2521 acgttcgttg caataataat cagcacgccg atacccaaaa gggcagtcag cgcacccaaa
     2581 atataaagcc aaaatgtgcg ccaaaaggtt ttaatcaggt attcggtatg tgcggcatag
     2641 acaatgctgt ccaaactgtt ccgtttcata taggcgagaa tcacgccgac tatgggcata
     2701 taggcgaaaa tcacgccgac tatgggcatg aacagcatga ttgcacccaa accgaacagg
     2761 atgtaggcgg cgaggacata gttgcgcccg cttccgccgc cggacggttc gtaagtatgg
     2821 cacgaacgtg atgcctgccg ttcaaacgcc tgtctgaacg cctgttcgcg gcgcatctgt
     2881 tcgcggtaaa actgctccct gcgcgcctgt tcgcggcgga atgcctcttc ctgccgcccg
     2941 cgttcctcat gccgtctgaa ggacgcgtcg tattgcatcc gtttcgacag gtcggaaagc
     3001 gtatcgtaag cccgttggat ttctttgaac ttttcttccg ccttcggatt gcccggattg
     3061 cggtcgggat gatatttcat cgccagcttg cggtaggcgc gtttgatttc gtccgctccc
     3121 gcctgcggcg acacgcccaa tacggcatac aaatctttgt ccatattgtt cctttatact
     3181 ttcccgtcaa accgccgcct gtattgtttt cgggttcaaa atatttaaat ctccgggacg
     3241 gatgccgata atgaacccgc gttttccgcc gttgatatag atggtttcca aatccatcac
     3301 cgactgttcg acgtaaatat ccaactttgt ccggatgccg aacggcgttg tgccgccgac
     3361 cagatagccc gtccacttgt ttgcctgtac gggcgtggcg ggttcaatgt gtttcgcacc
     3421 caaatgccgc gccagattgc gggttgaaat ctgcttgtcg ccgtgcatca agacaatcag
     3481 ccccttaccg ttttcatctt gcaaaacaat ggttttaatg accaagtgtt cgtctttgcc
     3541 gaatagtcgg gcaaactgcg ccgtgccgcc gtgttcctca taggcatagg taaaaggttc
     3601 aaattcgatg ccgttttcac gcaaaacgcg cacggcaggt gttatcggat aagtgtgttt
     3661 actcattttt cgtcatgcaa aacatggaaa tcggtcaagc gcaatgttac catatatccc
     3721 ctttatcgaa ggaaaccgat atggatatcc gatatttcgg cacaacgccc cgctattccg
     3781 aagccgtcgg cgcaaacggc ttgattttcc tctccggcat ggttcccgaa aacggtgaaa
     3841 cggctgccga acagactgcc gacgtgcttg cccaaatcga ccgctggctg gcggaatgcg
     3901 gctcggacaa agcgcacgtt ttggatgcgg tcatctatct gcgcgatatg ggcgactatg
     3961 cggaaatgaa cggcgtgtgg gacgcttggg ttgccgccgg cagaacgccc gcccgcgcct
     4021 gcgtggaagc acggctcgcc cgtccggagt ggcgcgtcga aatcaaaata accgccgtga
     4081 agagagacgc ggcaactgca taaacagatt tcctttatat ttcagagtac gcaaatgatg
     4141 aaaaccttac tgcccttttt cggcgtggtg cgcgtcagcg gcgaagaccg ccaaaccttc
     4201 ctgcacggac agctttccaa cgacatcaac caccttcaga ccgggcaggc gtgttatgcg
     4261 acttacaaca cgcccaaagg ccgcgtcatc gccaatatga ttgtcgtcaa ccgaggcggc
     4321 gatttgctgc tgattatggc gcaagacctg cttgaagcaa cggtcaaacg tttgcggatg
     4381 tttgtgttac gcgccaaagc cgtttttgaa atccttgaag attacgccgt cggcgcggaa
     4441 ttagaagcat ccgccgaacc gcttgccgcg caagaaccca gcctcgcctt taccgccgaa
     4501 tgcggttcag acggcatctg ttccgtcgtc ctgccacatc ggggcatttt gcacatcgcc
     4561 cccaaaaacg ccttgccgcc ttatgatgcc gccgccgaaa acgcgtggcg gctgcacgaa
     4621 atccgtagcg gttatccgtg gatatgcgcg gctaccaaag aaaccgccgt cgcccaaatg
     4681 ctcaaccagc acatcatcgg cggcgtacac ttcaaaaaag gctgctatcc gggacaggaa
     4741 atcattgccc gtgcccagta tcgggggcag gtcaaacgcg gcttggcggt attgtcggga
     4801 aattcggccg tcgaagcagg aaccctgctg acggcggacg gcgaagaggc cggcatcgta
     4861 ctcgacagcg ttcaagattc ggaaaacttt accgccctta ccgtgattaa attctccgcc
     4921 gcacaaaaaa cactgaccgt cccaaacggc ggtatcttca aagcggttca tctcttcttt
     4981 aaaaccgaaa atgcagaata agccgccgat gccgtctgaa gcccttcaga cggcattgcc
     5041 gccccgccaa tcggttacaa tgccgccttt ctaattttca aacccaaacc gccatgtcca
     5101 aaaaaaccaa acaagaactc gaaaacaaca aactcagcaa acgcctgcgc cacgccgtcg
     5161 gcgacgcgat taacgatttc aatatgatcg agccggacga caaaatcatg gtctgcctct
     5221 ccg
//
LOCUS       DAWHRA010000135         5207 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWHRA010000135 DAWHRA010000000
VERSION     DAWHRA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.135"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            188..1372
                     /gene="ubiM"
                     /locus_tag="WIB30_001789"
     CDS             188..1372
                     /gene="ubiM"
                     /locus_tag="WIB30_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ3797814.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(1643..1798)
                     /gene="rpmG"
                     /locus_tag="WIB30_001790"
     CDS             complement(1643..1798)
                     /gene="rpmG"
                     /locus_tag="WIB30_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ3797815.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1830..2063)
                     /gene="rpmB"
                     /locus_tag="WIB30_001791"
     CDS             complement(1830..2063)
                     /gene="rpmB"
                     /locus_tag="WIB30_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ3797816.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2313..3839)
                     /gene="farB"
                     /locus_tag="WIB30_001792"
     CDS             complement(2313..3839)
                     /gene="farB"
                     /locus_tag="WIB30_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ3797817.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     FVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSFVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(3863..5017)
                     /gene="farA"
                     /locus_tag="WIB30_001793"
     CDS             complement(3863..5017)
                     /gene="farA"
                     /locus_tag="WIB30_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ3797818.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         1347 a         1456 c         1238 g         1166 t
ORIGIN      
        1 ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca tacaccctcc
       61 cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac agatagttca
      121 aacggcattc aaacccttac aaataccgtc tgaaatcatc ggcatccgtc tatgcaaagg
      181 atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggtccgg cgggtttaag
      241 ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgatcgaac gcagcccact
      301 gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc atttttcccg
      361 cgaaatcatg cagcgtctgg gtatgtggga caaaattccc gaaaatgaaa tttacccttt
      421 gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc cccaaccgac
      481 tgaagcgcgc ggcgaacctg ccgactgttt gggctatctt atttccaacc acaatatccg
      541 acgcgctgcc tacgaagtcg tatcccaact cgacaacatt accattctga ccgatacggt
      601 tgtcaaagaa gtcaaaacat ctgacaacga agcacaagtc attttggaaa acggcaaaat
      661 cctgactgca cgcctgcttt tggcggcaga cagccgtttc tcacaaaccc gccgacaact
      721 cggtatctcc tccgatatgc acgattacag ccgaaccatg ttcgtctgcc gtatgaaaca
      781 taccctttcc aaccaacata ccgcttacga atgcttccat tacggacgca ccatcgcact
      841 gctgccgctg gaagagcacc tgaccaatac cgtcattacc gtcgataccg ataaaatcaa
      901 cagcgttcaa aacctttcgc ccgaagaatt ggcggcaagc gtaaaagaac aactcaaggg
      961 taggcttggc gacatggaac tggtcagcag catccaccat tacccccttg tcggtatgat
     1021 tgccaaacgt ttctacggca aacgcagcgc gctgatcggc gatgcggctg tcggtatgca
     1081 ccccgttaca gcacacggtt tcaacttggg gctttccagc gcagatattt tggcaaaact
     1141 gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt tggaaaaata
     1201 cagcagcaaa catatgctgc acgcccatcc gctttatcac ggaaccaata tgatgctgaa
     1261 actgtttacc aacgaaaccg cgccggcaaa actgctgcgc ggtttggtat tgcgtgccgg
     1321 caacaacttc ccgccgctga aaaagctgat taccaaacaa ctgaccggtt gaaaactgcc
     1381 gaaatgccgt ctgaaacgtt tcagacggca tttttgtatt atagtggatt aacaaaaatc
     1441 aggacaaggc gacgaagctg cagacagtac aaatagtacg gaaccgattc acttggtgct
     1501 tcagcacctt agagaatcgt tctctatcct tggcagacgg tttgcagcag attcgcatac
     1561 ggcagatgtt tcaagcggaa aagaaacacg ataaaaataa aaaagcctcc gaacagtcgg
     1621 aggctttgct ttcaaactga aattatttca gtttggtttc tttgtacact acgtgtttgc
     1681 gggcaaccgg atcgaatttt ttaatttcca gtttgccggg catagtgcgt ttgtttttgg
     1741 tagtggtgta gaagtgacca gtacctgcac tggattccag tttgatttta tcgcgcattg
     1801 cagtaatcct taattaaata gtgtttaaat taagcttcgc cgcgagcacg caaatcagcc
     1861 aatacgacat caatgcctac tttgtcgatg gtacgcagtg cagcgttgga aacgcgcagg
     1921 cgaacccagc ggttttcact ttctacccaa aaacgacgtg attgcaagtt gggcaaaaaa
     1981 cggcgtttgg ttttgttgtt ggcgtgcgat acgttgttgc cggacatcgg gcgcttaccg
     2041 gtcactttgc aaactcgtgc catggtcagt ctccaaactt ctaaaatagt aaaacgcgat
     2101 ttataccacg aaaaacagtg ttcgttcaag tgttttagag aaaaacggct gttttacgcg
     2161 aattatcctg ccgattgttg cattcgggaa acgtttgccg atacgaaaca gacggaatgc
     2221 gattatagcg ggtttcacag atgcctgcat tctaaatact gtccgaacat atttccagta
     2281 ttttcagacg gcatttcaag ttttcaaatc cctcaatgtc caccgccgcc gccgttgtgg
     2341 aacggcggtt ttgccagcca tatgacgggt atcatgataa tgaataacaa gctgcccgcc
     2401 ataaagattt cgttcgagcc gataataaaa ccctgctggg taatggtatt gttgatgatg
     2461 cctagggttt gaatgtcgga aacgccgtgc tgggacaaat gagcggccgt ttcgtgcaat
     2521 gttgcggaat agggcgtgat gtgttcggca aagcgtgtat ggtgcaacgc ttcacgccgt
     2581 tcccacaggg tactgacgaa tgatacgccg acaccgccca tcagcacgcg caagaaattc
     2641 gacaggctgc cggcggcggc aatctgcccg cccttcatat gcgacagtgt gatggtggtc
     2701 agcggcagga agaacatggc aacgcctgcc ccctgccaaa actgcggcca gatgacgttg
     2761 ccgatatcca tatcggcata aaaatccgta cgccaataga aagtaaaggc aaaggtcagg
     2821 aagctggcag ttacgaacag gcgcatatcg attttattgc cgaacctgcc gattaacgga
     2881 gacaggaaaa caggcaggat gccgacaggt gcggcggcaa gccctgccca cgtggaggta
     2941 tagcccaggt tggtctgcaa cactaacggc agcagggtca gcgtccccat atacaccata
     3001 aaacccaatg acgtggcaat gacgccgacg gtaaaattcc gatctttaaa cagcgataaa
     3061 tcgacaatcg gatatttttc tcccaattcc caaacaataa aatacgacaa gcacaccagt
     3121 gcgactacgc ccaaggtaat gatttctcca gaggcgaacc agtcgagttc cttacccctg
     3181 tccagcatca tctgtaacgc gccgataccg actaccatca atgtaagccc gacatagtcg
     3241 gtcggcattt taacggtttc cgtttcccga tatttcaaat gtttccatgt aatccatgcc
     3301 gatatgatac cgatagggat attaatgaag aaaatccaac cccaatgcca gtttccggaa
     3361 atccagccgc cgagtatcgg cccgagaaca ggggcgacaa cgacggtcat tgcccacaat
     3421 gccagtgcca gcgtccgttt tgcgggcgga taggatgcca ttaacaggct ttgcgacaag
     3481 ggaatcagcg gcccggcgat aaagccctgc aagatgcgga aaacaaccag cgactgaagg
     3541 ttgggggcaa taccgcacag ccacgatgtg atgacgaaac cgacagcggc ggcggtaaac
     3601 aatttgacct cgccgatgcg ttttgccaaa aagcccgtca gcggcacgga aacggcgttt
     3661 gccacagaaa aggaagtgat gacccacgtc ccctgagtgg ttgccgcacc gaggttgccg
     3721 gcgatgacgg gaacggcgac attggcgata gtcgtatcta aaacttccat aaatacggca
     3781 agccccaaag acagcgtaac ccacgccaat gccgcaccct taagcggtgg ataatccatt
     3841 gtgtcctccg tttcagacgg catcagggcg cggattgccc gaggatttca tcgaccgtcc
     3901 gatcgacttc cgaccagtcg gtactttcca tttccggcaa tgccgcaccc ggcgtttttg
     3961 aaacaggcgc gcctgcggcg gaagtatcca ctttaaccgt catcgacaaa ccgatacgca
     4021 acggatgcct gtccacatct tcgcggttca gcacgatacg gacggggacg cgctgcacca
     4081 ctttaatcca gttgcccgtt gcgttttgcg ccggaatcag cgaaaacgcg ctgcccgtac
     4141 ctgccgaaaa acctgccacc ctgccgcgat aaacaatttg tttgccgtac aaatcggaca
     4201 ccagctcggc aggctgtccg attttcatat gccgcaactg cgtctcttta aaattagcat
     4261 ccacccacac atccgacagc ggcaccaccg ccatcagcgg cgcgcctgcc gccacctgct
     4321 gcccgacctg caccgaacgc ttcgccacct gaccgtccgc cggcgcgcgg atttgcgtcc
     4381 gctgaaggtt caaccacgca tctttcaacc tgccgattgc cgtctgaacc gccggctgtt
     4441 cgcgcaaaga aacctgaccg cccaaagccg cacgtgccga agattcttcc gccaaagccg
     4501 ctttgaccgc cgcctgcgcc tgagacactg ccgcacgggc gtgtgccagc tcttcggcgg
     4561 acaccgcgcc cgattccgcc aaagcagacc ggcggcgcaa atcatcctgt gcgcgtgcca
     4621 aatccgcccg gcgcaaggca acctgcgccc ccgcctgcga agtggcggca ttttgccggc
     4681 ggttttgccg caccgcctga accagctcgt tttttgcccg ctcgtaagcc agcacatcat
     4741 tatcgtcgtc caataccgcc agcacgtcgc cttttttcac ggcatccgta tcgtcgtgca
     4801 aaaccttccg caccgtaccg cccttttgcg gcgtaacctg aaccacgcgt ccggcaacat
     4861 aagcgtcttc cgtttcctct tcgtgctgcc accataaaaa aaacgccgac ccggcggctg
     4921 cggcggcaag cgcgaacagc agcgtcaatg ccgtcaggcg gcgtttgcgt ttggcttgcg
     4981 tgttttgaag ttttgtttcg tccgtgtgcg tatccataag attgggttcc gaatttcctg
     5041 atttcatata aacaatgttc tttcaatagg aagcagattc tatatccgcg caataacgca
     5101 atcaagtcag tattttagat gcaatattca aaaaacaacg cttcaggcgg caaaactgaa
     5161 aatatatttc gtttatataa tggattaaca aaaatcagga caaagcg
//
LOCUS       DAWHRA010000136         5177 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWHRA010000136 DAWHRA010000000
VERSION     DAWHRA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.136"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..862)
                     /locus_tag="WIB30_001794"
     CDS             complement(<1..862)
                     /locus_tag="WIB30_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ3797819.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKAD"
     gene            complement(1231..2490)
                     /locus_tag="WIB30_001795"
     CDS             complement(1231..2490)
                     /locus_tag="WIB30_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ3797820.1"
                     /translation="MKQPVLQNNLQNLLESAENILLLQGPVGDFFLRLANWLTENGKT
                     VHKFNFNAGDDYFYPSTQAHTVVFNDSYDAFPEFLQEYIAQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKNGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVRGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVTYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGMTDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKNTSDSSTPAT"
     gene            complement(2627..4741)
                     /locus_tag="WIB30_001796"
     CDS             complement(2627..4741)
                     /locus_tag="WIB30_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ3797821.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLFTLLPEF
                     HICKDGKGAEAVIGWGLRPTTHKARAFAAEHQLPFIALEDGFLRSLGLGVAGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQAMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVNLLAEDINPISLLQNIDKVYCVTSQMGFEALLCGKPLTT
                     FGLPWYAGWGVSDDRHPKIDSLVQTQRRAPRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLAGVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAEAPSRLEHILQNQNFDDQDFQTALKLQKMLTENHISKYNVGNSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYHNLDLLRTVRERHPDAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQADILTCLQYADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGYFAKKLGKIKQLYRSFK"
     gene            complement(4765..>5177)
                     /gene="rfbC"
                     /locus_tag="WIB30_001797"
     CDS             complement(4765..>5177)
                     /gene="rfbC"
                     /locus_tag="WIB30_001797"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ3797822.1"
                     /translation="FVQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMR
                     EGSPTFGKWVGEILSAQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPKAEHSLI
                     WNDPTVGIDWPLQTAPNLSPKDLAGKVLAEAVTF"
BASE COUNT         1135 a         1074 c         1582 g         1386 t
ORIGIN      
        1 tgtcggcttt gataccgaga ccgtagcggt agaggtcgcc catgattttc agggcttcag
       61 gatggttttt ctcggcagcg gctttggcat atcgtgccgc ttggaacgga tcgcgttcgg
      121 aaagttcgcc ggtcagggcg tattgggcca gcttagactg tgcttccgct tgttccttat
      181 cggcggcacg acgataccat tccaaggctt caggtttgcg ttgggcggca agaaggtcag
      241 ccagcagcgt ttgtgcggcg atgtggccgg cttgtgcggc tggttcgaga tagtgttcgg
      301 cttgtgcgtg gttggggctg acacctgtac cgtagaagta gatttgacca agttgccagc
      361 aggcggcagt tacgccgagt gctgccgctt tttcgtattg ttcgatggct gtttcaacgt
      421 cgccttggcg ttgatgatgg cgtgccagat ggtaatacgc aggtgcggca tatcgggcgg
      481 cagcaagttc cgcccagtag agcgattgtg gatcgttgcg ttgcgcgtgg taacgcatga
      541 ggattaattg ggcttctgag tgcccctgct cacccagcca ggcatagtcg ttgagaagct
      601 gtccggtcgg ggtgccgttg tgttcgcgtt gttgcagcag ggagaagagt gctttgggat
      661 ggcgttgggc cgcggcggct tccaaccatg aaagtgcctg ctccggttgg ccgttttgca
      721 gcagatggtt gcccaatacg aaagcagctt ccgcataacc ttgctctgcc gcctgttgca
      781 acaggggcag ggcttgggcg gtattggatt gttccaaaag ggcggcggct tgccggtaga
      841 ggatatcagg agcggcggtc atggtttgcg ggcggatata aagtggaaga cggatgctgc
      901 ggcatatggg cggcgatacc ggttgagggc tttgtaaaaa ttttgctcgg cacagtttta
      961 tagagtgtat tgccttgccg gcttatcttt aggatgtttg gcttgttata ttgttttgcc
     1021 tagggatata tccatatttt gtgtgttaca aagggctcag gttgagtcgg tatgcgctca
     1081 tggcagctcg gtcattatac tgtttttgta atttattgtt aatgataaat gcagattttt
     1141 gtgttttatt tacatggcag tagctaaggt attgattgtt tcaggccgtc tgaaatgttt
     1201 tcagacggcc tgaacttttc cttcactaag tcaggttgct ggtgtagaag agtcggaggt
     1261 gtttttgttg gggaaaaaga cctgactgta gaagttgccg ttaatttggg tcacgttgag
     1321 gtggtacata cggtaggcat ggaacagctc tttgtcaggc ggtgtcggat gattccaaaa
     1381 ttctgccaag gtattttggt cagtcatgcc gggaatatca taataggcac ggccgagaac
     1441 cttaactggc atattgtgaa tcagtccgga caggccgctg gtgctgttga tagtaaccat
     1501 gccgaggccg tggcgcagga aaacgggcag ggggacatca tggacgtaag tcacgcggcc
     1561 tttgagttcg gggtgttctt tgataaagcg tttaatgtcg cgccagtagt cgataaagcc
     1621 gcggtccatc ggatgatgct tgatgatgat attggtatcg gcaggcgcgt gctcggcaaa
     1681 tgaactcaaa acatggagca ggaagctgcg gacgctggga aagtcgcaat ggatacggac
     1741 ttggctgtcg ttgaatacct gtaagggaac aataaaaaac ttgccgtatt tgcctgcttc
     1801 cacacgtttg gcgatttgga tgtcttcaat atagtagttc aaacgcttga ggatggagag
     1861 cgaccacggt ttgaggtaat ggccggcatt gggtgcgcgg tggtggatgt agtcggggta
     1921 tttgcgtgga ttgcggaaca actcgatata gtaacggata gcgttttttg ccatgggcgt
     1981 aaaaccgccg cgtaccggcg tcggcgcttt gtattcttgc tgggccagct tggggaattg
     2041 ttccaaaaaa aagtcggcac ggcgcggcaa cggggaaaat gcgttgacgc cgtttttttc
     2101 taaggtgatg tagtaggggc ggaaatagcc ttcttcaaac gcccagaaac tggcttggtt
     2161 ttcgtttgca atgcgttttg caatgacgtg ataagggcgt gtgtcgccaa agcagacaac
     2221 cgcctggatg tgatgttgag cgatgtattc ttgcaaaaat tcaggaaagg catcgtagct
     2281 gtcgttaaaa acaacggtat gcgcttgagt ggacggataa aaatagtcgt cgcctgcatt
     2341 aaagttgaat ttatgtacag ttttgccgtt ttcagtcagc caattggcaa ggcgcagaaa
     2401 aaaatcgccg acaggacctt gaagcagcag gatattttct gcgctttcaa gcaggttttg
     2461 caggttgttt tggaggacgg gctgtttcat gtcgcaatgt gattttgttt tttatgtaat
     2521 agttttgggt tgaactttca agcatacgcc aagagaatta acgatgcaaa ggcaatgatg
     2581 ccgcaattga agctggttta tgacgaatcg ttaactttga tggtatttat ttgaaagatc
     2641 tatatagttg tttgatttta cctaattttt tggcaaagta cccgcgatgt aatccgttgt
     2701 tgtttttttg catatttttt tgtcgtatca ggatttgggc agcggtttcc gcattgatgg
     2761 cctgatgggt ttcggggtgg atgtagtcgg gatagtgtat gagcgtgccg gcaatcagct
     2821 gccaaagctc aagtctgcgg ctacggcgcg gaatggggag cagatcttgg gtaagtcccc
     2881 agcctgcgta aaaaggcagg ccgtagcagc tgactttttt gccgcgcaac aaggcttcaa
     2941 aaccggtcag cgaagtcatg gtatgtattt cgtctgcgta ttggagacag gtcaggatgt
     3001 cggcttgttc ggcggtttgg tcggcatatc gtgcagcatc ttcaggggaa atatggccga
     3061 tgcggttacc gctgactaca tcgggatgcg gtttgtagat gatataggca tcgggatggc
     3121 gttcgcgtac ggtacggagc aaatccagat tgtgatagat ttggggcgaa ccgtagcgga
     3181 tagacgcatc atcctcaacc tgaccgggaa cgaggatcac ggttttgtcg gttgatgggg
     3241 cggtaaagtc tgagttgccg acgttgtatt tgctgatatg gttttcggtc agcatttttt
     3301 gcaacttcaa ggccgtctga aagtcttgat cgtcgaagtt ttggttttgc aggatgtgtt
     3361 caaggcggga tggggcttcg gcattgaaat aaatgctcat atcgtcggta acgagcgaca
     3421 gcggcggcac taagttggag ccgagtccga ccgagcggat aaagccgtct tccatgcgca
     3481 gcagggggat gtggtgttgt tcggcaaagc ggacgatggc ctctttgccg ttgccccaag
     3541 ccaggatgcg tgcatcgtcg gacagtttga ccccggccag tttttgggtg gaagagataa
     3601 atttcagacg gcaagagggt acgttaaaga acggtttggc aaccgcgcgt ttccacaaag
     3661 acataccgac gcaatataac tcgccacgca atttgtcgtt tttacgtttg accgtcgcca
     3721 gatagtcgat gacatcaaag aggctgcctg cttcgccggt attggggttg aggtagcggc
     3781 tgtattgcag ataggctgca gcgaagagct gcagcaagtt gcggggggcg cggcgttggg
     3841 tttgaacaag gctgtcgatt ttaggatggc ggtcgtcact cacgccccat ccggcatacc
     3901 acggcaggcc gaaagtggtc agcggcttgc cgcacaaaag cgcctcaaaa cccatttgcg
     3961 aggtaacgca ataaacttta tcgatgtttt gcaacaaaga aatcggattg atgtcttctg
     4021 ccagaagatt gacgcggtgt tgctgcgcca gttgggtcag atagccttgt tttttgccgc
     4081 acaaaacatc gggatgggtt ttgacccaaa tatcggcttg cgggttttca tttaaggccg
     4141 tctgaaacat cagttcaaac gtagaggcgt ctgcgccgcc atattggatg gccatatcgc
     4201 cgaatgtttg gtcgataatg aggacggttt caggtttgga tggggagcgt aaaggatgat
     4261 cgtctgaaag ttcgggcgcg tggttgtatt tggacaggtg gtgttgcagg atgaaatcca
     4321 tcgcctgctg cgcctgagcc aaggtttcag acggcatggt atcggcggca agaatcagtt
     4381 gttccaaacg cgaaggacgt gtggtgtcgt agtagatgcc gatgtcgtca tagacgatgg
     4441 aatagggagg atagccggca acgcccaggc caagcgagcg taaaaagccg tcttccaaag
     4501 caataaaggg aagctggtgt tcagcggcaa aagcacgcgc tttgtgtgtc gtcgggcgca
     4561 aaccccagcc gataacagcc tctgctcctt tcccatcctt gcagatatga aactcaggca
     4621 atagggtgaa gagatggggg attttgcgga tgccacgaga ggggatgtag gcgtttttca
     4681 caatgcgttt tcgtcgatta ttgttattaa tggattgtag gccgtctgaa aagaggaaca
     4741 ttctttcaga cggcctgaat agtcttaaaa cgtgactgct tcggccaata ctttgcctgc
     4801 caagtcttta ggcgacaggt tcggcgcggt ttgaagcggc cagtcgatgc cgacggtcgg
     4861 atcattccaa attagtgagt gttcggcttt ggggttgtaa tagtccgtgc atttgtacac
     4921 gacttccgct gcgtcgccca aaacgcaaaa cccgtgcgcg aagccttcgg gtatccaaag
     4981 ctggcattgg ttttgcgcgg acagaatctc gcctacccat ttgccgaaag tcggcgaacc
     5041 ttcgcgcata tcgacggcca cgtcgaacac ttcgccgaca actacgcgta cgagtttgcc
     5101 ttgtgtgttt tcggtttggt agtgcaggcc gcgcaatacg cctttgccgg atttggagtg
     5161 gttttcctgc acaaagg
//
LOCUS       DAWHRA010000137         5064 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWHRA010000137 DAWHRA010000000
VERSION     DAWHRA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.137"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(32..1516)
                     /gene="tldD"
                     /locus_tag="WIB30_001798"
     CDS             complement(32..1516)
                     /gene="tldD"
                     /locus_tag="WIB30_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ3797823.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      1694..1797
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1864..3087
                     /gene="cytX"
                     /locus_tag="WIB30_001799"
     CDS             1864..3087
                     /gene="cytX"
                     /locus_tag="WIB30_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ3797824.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIESFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            3084..4184
                     /locus_tag="WIB30_001800"
     CDS             3084..4184
                     /locus_tag="WIB30_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ3797825.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIVLFDKGCRRGENAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQTQYDWLIDCRGYGAKTAWNQAPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            4206..4823
                     /gene="thiE"
                     /locus_tag="WIB30_001801"
     CDS             4206..4823
                     /gene="thiE"
                     /locus_tag="WIB30_001801"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ3797826.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDE
                     LKREIARCVAACQGSRTQLFINDHWHEAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELNRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          994 a         1545 c         1446 g         1079 t
ORIGIN      
        1 cctttcagac ggcatcgtgc cggattccgc gtcagattgc gctgccgccg acggtcagtc
       61 cggcatcgat acgcagggtc ggttgcccca cgccgacggg gacgctctgc ccttctttgc
      121 cgcacacgcc gacaccgctg tcgagcgcgg tatcgttgcc tatcatcgaa acgtgtttca
      181 gcacttcggg accgttgccg ataatggtcg cgcctttgac gggatattgc agcctgccgc
      241 cttccaccca ccatgcttcg gacgcgctga acacgaattt gccgctggta atgtccacct
      301 gtccgccgcc gaagttgacg gcgtaaatgc ctttgtcgat ggacgcgatg atttcttccg
      361 gctcatagct gccgttttcc ataaaggtat tggtcatgcg cggcataggg gcggaagcgt
      421 aactttcgcg gcggccgttg ccggtggatt gcgtacccgt caggcgggca ttggtttcgt
      481 cctgcatata gccgactaaa atgccgtctt caatcaatac ggtgcggcgg gtttcgttgc
      541 cttcgtcgtc gatgttgagc gaaccgcgcc ggccggcaat atcgccctga tcgacgacgg
      601 taacgccttt ggcggcgacg cgctcgccta ttctgccgga aaagacgctg gtccccttgc
      661 ggttgaaatc gccttccaaa ccgtgtccga ccgcttcgtg cagcaacacg cccggccagc
      721 cgttgcccaa aacgacggtc atttcgccgg cgggcgcggg gcgggattcg aggttggtga
      781 gtgcctgttt gacggcggca tcgacaaact gccgcaccaa gttttcatcg aaataagcca
      841 agtcgtagcg tccgccgccg cccgcgctgc cctgttcgcg gcgttcgccc tgtttggcga
      901 tgacggtaac gttcaggcgc accatcgggc ggatgtcggc ggcgtgtttg ccgtccagac
      961 gggcgaggta aaccatatcg tattcgcagg tcaaaccggc catcacttgc acgatgcgcg
     1021 gatcggcggc tttggcgatt gcttccactt tgttcaacag cgcgactttg gcggcggaat
     1081 cgaggccggc aatggggtcg gacgcggaac aaaccggctt gccgcgcgtt tcagacggca
     1141 ttttggcgga caccttgccg cctgccgccc caatcgcgcg gacggcgcgg gcggaacggt
     1201 ttatcgaatc gatgcacagg ctgtcggcgt aggcaaaggc ggttttgtcg cccgaaacgg
     1261 cgcgcacgcc cacgccctga tcgatttgga agctgcccga tttgacgatg ccctcttcca
     1321 aatgccagct ttcataggcg gtgcgctggc agtagatgtc ggcgtaatcg acgtggtgcg
     1381 cgccgatgat gcacaggctt ttggcgagca gttcggggga aaggcggttg gcttcgagca
     1441 gccgcgcctg tacggcggaa taggtcggat gcatagtgtc ggcgcataaa aaatcagggg
     1501 cttgattata cggcatttgt tatatagtgg attaacaaaa aacagtacgg tgttgcctcg
     1561 ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
     1621 cactatagaa atgcgccgtg ccgccgaaaa tgtaagaatt ttgccaacgc acgttgcttt
     1681 gtgtaaactt aaagctttct tgtcggagtg ccgccgccgg gcggctgaga ttgcgaaagc
     1741 agaatccgta gaacctgtcg gggtaatgcc tgcgtaggaa acaaaccgtc aaatgcctta
     1801 tcaggcttcc gttccctttt ccgcacttcc ccgcccacct tccttctttt taaaggacgt
     1861 catatgtcgg gcaatgcctc ctctccttca tcttccgccg ccatcgggct gatttggttc
     1921 ggcgcggcgg tatcgattgc cgaaatcagc acgggtacgc tgcttgcgcc tttgggctgg
     1981 caacgcggtc tggcggctct acttttgggt catgccgtcg gcggcgcgct gttttttgcg
     2041 gcggcgtata tcggcgcact gaccggacgc agctcgatgg aaagcgtgcg cctgtcgttc
     2101 ggcaaacgcg gttcagtgct gttttccgtg gcgaatatgc tgcaactggc cggctggacg
     2161 gcggtgatga tttacgccgg cgcaacggtc agctccgctt tgggcaaagt gttgtgggac
     2221 ggcgaatctt ttgtctggtg ggcattggca aacggcgcgc tgattgtgct gtggctggtt
     2281 ttcggcgcac gcaaaacagg cgggctgaaa accgtttcga tgctgctgat gctgttggcc
     2341 gttctgtggc tgagtgccga agtcttttcc acggcaggca gcaccgccgc acaggtttca
     2401 gacggcatga gtttcggaac ggcagtcgag ctgtccgccg tgatgccgct ttcctggctg
     2461 ccgctggccg ccgactacac gcgccaagca cgccgcccgt ttgcggcaac cctgacggca
     2521 acgctcgcct acacgctgac cggctgctgg atgtatgcct tgggtttggc agcggcgttg
     2581 ttcaccggag aaaccgacgt ggcaaaaatc ctgctgggcg caggtttggg tgcggcaggc
     2641 attttggcgg tcgtcctctc caccgttacc accacttttc tcgatgccta ttccgccggc
     2701 gcgagtgcga acaacatttc cgcgcgtttt gcggaaacac ccgtcgctgt cggcgttacc
     2761 ctgatcggca cggtgcttgc cgtcatgctg cccgttaccg aatatgaaaa cttcctgctg
     2821 cttatcggct cggtatttgc gccgatggcg gcggttttga ttgccgactt tttcgtcttg
     2881 aaacggcgtg aggagattga aagctttgac tttgccggac tggttctatg gctggcgggc
     2941 ttcatcctct accgcttcct gctctcgtcc ggctgggaaa gcagcatcgg tctgaccgcc
     3001 cccgtaatgt ctgccgttgc cattgccacc gtatcggtac gccttttctt taaaaaaacc
     3061 caatctttac aaaggaaccc gtcatgaccc gtatcgccat cctcggcggc ggcctctcgg
     3121 gaaggctgac cgcactgcag cttgcagaac aaggttatca gattgtactt ttcgataaag
     3181 gctgccgccg gggcgaaaac gccgccgctt atgttgccgc cgcgatgctc gcgcctgcgg
     3241 cggaagcggt cgaagccacg cctgaagtca tcaggctggg caggcagagc attccgcttt
     3301 ggcgcggcat ccgatgccgt ctgaacacgc acacgatgat gcaggaaaac ggcagcctga
     3361 ttgtgtggca cgggcaggac aagccattat ccagcgagtt cgtccgccat ctcaaacgcg
     3421 gcggcgtggc ggatgacgaa atcgtccgtt ggcgcgcaga tgaaatcgcc gaacgcgaac
     3481 cgcaactcgg cggacgtttt tcagacggca tctacctgcc gaccgaaggc cagctcgacg
     3541 ggcggcaaat attgtctgca cttgccgacg ctttggacga actgaacgtc ccttgccatt
     3601 gggaacacga atgtgccccc gaagacctgc aaacccaata cgactggctg atcgactgcc
     3661 gcggctacgg cgcaaaaacc gcttggaacc aagcccccga gcacaccagc accttgcgcg
     3721 gcatacgcgg cgaagtggcg cgggtttaca cacccgaaat cacgctcaac cgccccgtgc
     3781 gtctgctcca tccgcgttat ccgctctaca tcgccccgaa agaaaaccac gtcttcgtca
     3841 tcggcgcgac ccaaatcgaa agcgaaagcc aagcccccgc cagcgtgcgt tcagggttgg
     3901 aactcttgtc cgcactctat gccgtccacc ccgccttcgg cgaagccgac atcctcgaaa
     3961 tcgccaccgg cctgcgcccc acgctcaacc accacaaccc cgaaatccgt tacaaccgcg
     4021 cccgacgcct gattgaaatc aacggccttt tccgccacgg tttcatgatc tcccccgccg
     4081 taaccgccgc cgccgtcaga ttggcagtgg cactgtttga cggaaaagac gcgcccgaac
     4141 gcgatgaaga aagcggtttg gcgtatatcc gaagacaaga ttaaagccgc ccgaaaggac
     4201 accttatgac cttcccgccc ctaaaatccc cgctcaaatt ctacgccgtc gtccccaccg
     4261 ccgattgggt ggggcgcatg gtcaaagcag gtgccgacac ggtgcaactg cgctgcaaga
     4321 ccctgcacgg cgatgaattg aaacgcgaaa tcgcccgctg cgtcgccgcc tgtcaaggca
     4381 gccgcacaca gcttttcatc aacgaccact ggcacgaggc aatcgaagcg ggcgcatacg
     4441 gcgtacatct cgggcaggaa gacatggaca ccgccgacct tgccgccatc gccgccgccg
     4501 gtttgcgttt aggtttgagt acgcactccg tcgccgaact caaccgcgcc ctgtccgtac
     4561 atcccagcta cgtcgccagc ggcgcgattt tcccgaccac gaccaaacaa atgcccaccg
     4621 ccccgcaagg cttggacaaa ctgtgcgagt atgtgaaaca ggcaggcggc acgcccgtcg
     4681 tcgccatcgg cggtatcgac ttgaacaacg cccgagccgt actcgccacc ggcgtttcct
     4741 cactcgccgc cgtccgcgcc gtaaccgaag cggcaaatcc cgaagcggtg gttaaagcgt
     4801 ttcaggcttt gtgggatgga taaaaccgaa agaagaaaat tcaattgccg tgtaggcaaa
     4861 acttagcccg ttatcgcaaa catacttaac tttaaatgtg gcatatcatc aaattccgtc
     4921 attcccgcgc aagcgggaat ccgccttaaa acttgagaaa ccatcatttg aaaaacagtt
     4981 tccgaatttc aaaaatggat tcccgcctgc gtgggaatga cggcaaccgg ccggttgcgt
     5041 atcaaaaaat aaagtaattc ggct
//
LOCUS       DAWHRA010000138         4829 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWHRA010000138 DAWHRA010000000
VERSION     DAWHRA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.138"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..994)
                     /locus_tag="WIB30_001802"
     CDS             complement(<1..994)
                     /locus_tag="WIB30_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEZ3797827.1"
                     /translation="MNTVSNYLSALREAMRAQGLDALVIPSADPHLSEYLPAHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIEHPNDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIG"
     gene            1191..1391
                     /locus_tag="WIB30_001803"
     CDS             1191..1391
                     /locus_tag="WIB30_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797828.1"
                     /translation="MPFFGSNTENIVKFEHEVLIVCVGRYLDYVTIRAFIKISLPGGI
                     LFLKTVGFGFVWGQSRPNWSNR"
     gene            1452..2705
                     /locus_tag="WIB30_001804"
     CDS             1452..2705
                     /locus_tag="WIB30_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ3797829.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            2860..4371
                     /gene="lysS"
                     /locus_tag="WIB30_001805"
     CDS             2860..4371
                     /gene="lysS"
                     /locus_tag="WIB30_001805"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ3797830.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDTEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(4448..4636)
                     /locus_tag="WIB30_001806"
     CDS             complement(4448..4636)
                     /locus_tag="WIB30_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ3797831.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPKISRMLSSILAGYAWDTENPFVAK
                     SEQRLSALSEWVGQLETE"
     gene            complement(4640..>4829)
                     /locus_tag="WIB30_001807"
     CDS             complement(4640..>4829)
                     /locus_tag="WIB30_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797832.1"
                     /translation="RLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSVIFGVVLEVKK
                     RFMSAAQARRRVDSDYRP"
BASE COUNT         1107 a         1261 c         1333 g         1128 t
ORIGIN      
        1 tgccgatgat gtcttcaaac tcggcgaaga aaccgcacaa cgccgcgccg tcgtgttcca
       61 tcgcttcgcg gatgcgggcg atgtcggctt cggatttgca ggatttgaac agcgtggatg
      121 ggttgattcc ctcgataagg cgcacgcttt cgggcaggcg cacaagcgtg ctgacggcgg
      181 ttttgttcgg ttcgataagc agcgcgccgc cgatttgcgc gagtttgtcg gcaacttggg
      241 cataaggttc gaccgtgatg cctgcggttt gcagcgcggc ggcggcttcg gcgttcagac
      301 ggcatcggtc ggtaaacagg acggcgttgt ctttgccaat cagcaggaag gacacgaaaa
      361 cagggttgaa aggtacgtcg ctgccacgca ggttggtcag ccaagcgatg tcgtcaagcg
      421 aggaaaccaa gtggtaatcc gcgccttttt ccgccatcac ggcgcgcacg cgggcgagtt
      481 tttcggcggc ggtttcggag acgtaggcgg ggtcgtggat gaacacggtt tcggcaggaa
      541 tggccgggcg gcttgtccac acttggtcaa gcagatcgtt tgggtgttcg atgcggatat
      601 ttttggcggc gagtgattgc gccaaagtgc gtttgccggt gagcgagacc atatcggaag
      661 ggatgccgac ggcggcgttt tcgggcaggt ttgccgcgag ccattcgttg tacggcggca
      721 cttgaccgct tttttgcagc tcgatgccgc tgccggaaag ctgtttggcg gcttgttccc
      781 aatagcggct gtccacccat acacccgctt catcggtggt caggacaaat gtgccgaccg
      841 agccggtaaa gcccgacaat tcgcggcgcg cctgccagtg tgcgggcagg tattcggaca
      901 ggtgggggtc ggcggaaggg atgacgagtg cgtccaagcc ttgcgccctc atggcttcgc
      961 gcaatgcgga caggtaattc gatacggtat tcataacagt tcctccaatc gggttttgcg
     1021 gcttcagacg gcatggacgg aaatctgaaa tgccgtctga aaaacataac catcttatca
     1081 aatcgccagc ctgctgcaaa acggacgggc tgatcggatg gtgcgtgttt gcgcaaaaaa
     1141 agcgatgtaa ggttgtttgt tgtaaaattt taggcataaa atgtcgtttt attccttttt
     1201 ttggaagtaa tacggaaaat atagtcaagt ttgagcatga agttctaata gtgtgtgttg
     1261 gcaggtattt ggattacgtt acaatacgcg ctttcatcaa aatttcccta ccgggcggta
     1321 tcctgttttt gaagacggta ggatttgggt ttgtttgggg gcaatcccgc ccaaattgga
     1381 gcaatagata gggctgtgtc cgaatatgcg gctctgtgtg ttgaaacaca gttaaaaata
     1441 cggaagactt tatgtccgaa caacatattt cgacttggaa aagtaaaatc aacgcattgg
     1501 gtccggggat catgatggct tcggcggcgg tcggcggttc gcacctgatt gcctcgacgc
     1561 aggcgggtgc gctttacggc tggcagatcg cgctcatcat catcctgacc aacctcttca
     1621 aatacccgtt tttccgcttc agcgcgcatt acacgctgga cacgggcaag agcctgattg
     1681 aaggttatgc cgagaaaagc cgcgtttatt tgtgggtatt cctgattttg tgcatcctct
     1741 ccgccacgat taacgcgggc gcggtcgcca ttgtcaccgc cgccatcgtc aaaatggcga
     1801 ttccctcgct gatgtttgat gccggcacgg ttgccgcctt gattatggca tcctgcctga
     1861 ttattttggt gagcggacgt taccgcgctt tggatcgcgt ttccaaaatc atcatcgtta
     1921 ctttgagtat cgccacgctt gccgccgccg gcatcgctat gtcgcgcggt atgcagatgc
     1981 agcccgattt tatcgagccg acaccgtgga cgcttgccgg tttgggcttc ctgatcgcgc
     2041 tgatgggctg gatgcccgcg ccgattgaaa tttccgccat caattctttg tgggtaaccg
     2101 aaaaacaacg catcaatcct tccgaatacc gcgacgggat ttttgatttt aacgtcggtt
     2161 atatcgccag tgcggttttg gctttggttt tccttgcact gggtgcgttt gtgcaatacg
     2221 gcaacggcga agcagtgcag atggcgggcg gcaaatatat cgggcaattg atcaatatgt
     2281 acgccgttac catcggcggc tggtcgcgcc cgctggtggc gtttatcgcg tttgcctgta
     2341 tgtacggcac gacgattacc gttgtggacg gctatgcccg tgccattgcc gaacccgtgc
     2401 gcctgctgcg cggaaaagac aaaacgggca acgccgaatt ctttgcctgg aatatttggg
     2461 tggcgggcag cggtttggcg gtgatttttt ggtttgacgg cgtaatggcg aatctgctca
     2521 aatttgcgat gattgctgct tttgtgtccg cccctgtgtt tgcctggctg aattaccgtt
     2581 tggtcaaagg tgatgaaaaa cacaaactca catcaggtat gaatgccctt gcattggcag
     2641 gcttgattta tctgaccggt tttaccgttt tgttcttatt gaatttggcg ggaatgttca
     2701 aatgataacg atgccgtctg aaaccgcaaa ccgctttcag acggcattgc cgcgtttatg
     2761 gaaaaaaatg ccgacatcgc gtaaaatatg cgcaaatttt gtgattcggt caggtcgtct
     2821 gaaacagatt gcgcctgatt tattttttcg gaaaacccta tgagcgaaca aaaccatccg
     2881 caaaccgagc cgcagttgga cgaaaaccaa atcatcgccc tgcgccgcga aaaactgcac
     2941 aacatccgcc aacagcgcaa cgcctatccc aacgacttca aacgcgacag cttcgccgcc
     3001 gatttgcacg cccaatacgg cgaaatcggc aaagaagaac tcgatccgca aggcattccc
     3061 gtcaaagtgg ccggccgcat gatgctgaag cgtcaaatgg gcaaggcgag ctttgccact
     3121 attcaagacg tgtccggaca aatccagctt tatctgaaca acaaaggcgt gagccaagaa
     3181 gttttggacg acttcaacca ttgggatttg ggcgacatcg tcggcgcgga aggcactttg
     3241 ttcaaaacca accacggcga actgaccgta cgcgtgtccg gcatccgcct gctgtccaaa
     3301 tccctgcgtc cgctgcccga caaacacaaa ggtttgagcg atcaggaaac caaataccgc
     3361 caacgctatg tcgatttgat tgccaacgaa gaatcgcgca atacctttat caaacgcagc
     3421 caaatcatcc aatccgtgcg taattttatg gtgggcgagc attatctcga agtcgaaacc
     3481 ccgatgatgc acccgattcc cggcggcgcg acggcaaaac ccttcgttac tcatcacaat
     3541 gccttagata ttccgcttta cctgcgtatc gcgcctgagc tgtatttgaa acgcctggtt
     3601 gtcggcggct tggaacgcgt gtttgaaatc aaccgcagct tccgcaacga aggcatgtcc
     3661 gtgcgccaca accccgaatt tacgatgatt gaattttacg aagccttctc cgactacgaa
     3721 cgtatgatgc agatggcgga agacatcatc cgcaacgcat cgcgcacagt aaacggtacg
     3781 gcaaacatca cttacaatgg caaagaagtc gatttggaaa gcccgttcga gcgtctgacc
     3841 atcctcgaag ccatcaaaaa atacaatccg cactacaccg acgagcagtt gaacgatacg
     3901 gaatggctga aaaaagaaat cgtcaaacac ggtgaaagcc tgccgccgtc cccgggcatc
     3961 ggcagcctgc aactcgcgct gtttgaaggt tgcgccgagg gcaagctgtg gaacccgacc
     4021 tttattgtcg attacccggt cgaagtttcc ccgttggcac gcgcttcgga tacgaaacaa
     4081 ggtctgaccg aacgcttcga gcttttcgtt gtcggccgcg aactggcaaa cggctattcc
     4141 gagttgaacg accccgaaga ccaagccgaa cgcttcaaag cgcaagtggc gcaaaaagac
     4201 gcgggcgacg acgaagcgat gcactacgat gccgactaca tccgcgcgat ggaattcggt
     4261 ttgccgccga ccggcggttg cggcatcggc atcgaccgct tagtgatgct gctgaccgat
     4321 tcgcaaacca tccgcgatgt gattctgttt ccgcagatgc gccccgagta atcataaaaa
     4381 cagttgaaat agaaatgccg tctgaacccg attggattca gacggcattt tgtatggcgg
     4441 tacggattta ttcggtttcc aactgaccga cccattcgga caaggccgac aggcgttgtt
     4501 cggatttcgc cacgaacggg ttttcggtat cccacgcgta gcctgccaaa atcgaagaaa
     4561 gcatacggct gattttcggg ctgcggtcgg gcgcgtacac gacgttttgg aagcggaaat
     4621 cgtaccaacc gtccacatat caggggcggt aatccgaatc aacacgcctt ctcgcctgtg
     4681 cggcggacat aaaccgtttt ttgacctcca gcaccacacc gaaaatcacg ctgccttcgg
     4741 gcaggtcaaa ggcttcagac ggcctgcccg tctgataaat cagcatcctg cccgcaatat
     4801 cgataacaag gtgcgcgacg accagccgc
//
LOCUS       DAWHRA010000139         4781 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWHRA010000139 DAWHRA010000000
VERSION     DAWHRA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.139"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(79..315)
                     /locus_tag="WIB30_001808"
                     /pseudo
     CDS             complement(79..315)
                     /locus_tag="WIB30_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            511..969
                     /locus_tag="WIB30_001809"
                     /pseudo
     CDS             511..969
                     /locus_tag="WIB30_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            869..1237
                     /locus_tag="WIB30_001810"
     CDS             869..1237
                     /locus_tag="WIB30_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ3797833.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1306..2844
                     /gene="lnt"
                     /locus_tag="WIB30_001811"
     CDS             1306..2844
                     /gene="lnt"
                     /locus_tag="WIB30_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ3797834.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASNNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMDMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3108..3971
                     /gene="rpoH"
                     /locus_tag="WIB30_001812"
     CDS             3108..3971
                     /gene="rpoH"
                     /locus_tag="WIB30_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ3797835.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(4030..4176)
                     /locus_tag="WIB30_001813"
     CDS             complement(4030..4176)
                     /locus_tag="WIB30_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797836.1"
                     /translation="MLIHYINFANLSEKQLMRQCLVHVCFLISAIAMAWTDKIVYGTT
                     HKPH"
     gene            4455..>4781
                     /locus_tag="WIB30_001814"
     CDS             4455..>4781
                     /locus_tag="WIB30_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797837.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKNLR
                     KHHDLAK"
BASE COUNT         1195 a         1457 c         1093 g         1036 t
ORIGIN      
        1 cgaccttgcc gtcgggcagg atgtaccacg ccaaatcccc gcctgaagca cggtgtcgga
       61 ctgcatttct attcggctat acgccttttc acgcaggctg taattgaatt tccacaaacc
      121 gccgaacact gccgacggac gcgcgaccag catcaggaac gccgcaatcg ccaaggcttc
      181 cgccgccctg tccaacacgt tggcaggaga aaccagcagc aaaggcattc ccaaacgtgc
      241 ggacaaagtg gtcgaaacca cgctcagaaa caacagtgcg ccacccggca ggaacagact
      301 gcccatccaa tccacgctaa cgattctccc ttaaaattca tgaaattaaa atcgaaagac
      361 tgtaacacgg tattttcttt tcacgaaact ttacttcacg tccgccacag cgttcagacg
      421 gcattttcct gtttcatgcc gcctgaaacc gcccaaatcc gcgttctcct atataatgtc
      481 ggactttgcg ccgcatttca ggattcttta atggcatttg cctcactatt taccctgccg
      541 gacgacatca ccgccgtttt ggacgatgtt gccctgatga ccaaagccgc cgcaaagaaa
      601 accgccggcg tggtcggcga cgatttggca cccaatgcca accgggttac gggcgtatcg
      661 gcagaacgcg agttgccgat tgtttggtcg atggcgaaag gttctttttc caacaagccg
      721 gttttggtgc ctgcggccct gctgctttcc gccttcctgc cgcaactcat tacgccgctt
      781 ttgacggcag gcggcattta tctgtgcttt gaaggcgtag aaaagctgct gcacaagttt
      841 ctgcaccgac atgacgcgca tgctcgtaat ggcggcaatc ggcatcggca tgactgtttt
      901 ggtgtacgga atcgttgccg tcatcgtcaa actcgacgac ttaggcatgc tgctgatgcg
      961 ccgcccacaa accttttcgc gcagtttggg gcaaatgctg actgccttta tgccttgctt
     1021 tatgcgcggt ttgagcgtgg tcggcacgct tgccatgttt cttatcggcg gcgtactggt
     1081 cgcccacaac ctcggccttc tgcacgattt cctgcacgcg caacactggg acgctggctg
     1141 ggcggagtac ttcgccaacc tcgttgtcgg gctgctttcc ggttcgattg cctgcgcccc
     1201 cgccttgccg ctgatgaatc gtttcggcag gcattgattc ctttttcaaa taccgatgcc
     1261 gtttgaaaga tgttcagacg gtatcttccg aacagacaga tgaatatggt ttccaaacta
     1321 gacaaatact ggcagcaccc cgccctctac tggcctttgc tcatcctttt tgccgccgcc
     1381 acccccttta ccttcgcacc ctactaccac ttttggctga tgcccttgat tttcggtgcc
     1441 ttcgtccgcc tcatcgaact gcgtccgcgt tttgctgtct cttccgccta cctgttcggc
     1501 ctgaccgcat acacgacaca gttctactgg atacacaccg ccctgcacga cgtttccggc
     1561 ctgcccgacc tctatgccgt accgctgacc ttcctactcc ccgcctacct tgccctttat
     1621 ccggcactgt gtttctggct gtggaaaaaa tttaccctgc ctcggggcat aaaaatcggt
     1681 ttggtactgc ccatcctgtg gacgctgacc gagtttgccc gcgaacgttt cctgaccgga
     1741 ttcggctggg gcgcaatcgg ctactcccaa atcaccccgg acagcccgct cgccggcttt
     1801 gccccattgg gcggcatcca catggttaca ctggcaaccg cctttctcgg tgtctggctg
     1861 gttttggcga gtaataacac cgcacgttcg ggcaaacgcc tgcttccgat tatcctgatt
     1921 gccgccctgc ttgccgcagg ctacaccgcc cgacaaaccg acttcacccg ccccgacggc
     1981 agccgcagca ccgtcgccct gcttcaaggc aacatcgacc aaaccctcaa atggcgtgaa
     2041 gaccaagtta tcccgaccat acagaaatat tacgaacaag tcggcaaaac caccgccgac
     2101 atcgtcatcc tgcccgaaac cgccatcccc gttatgcgcc aaaacctgcc ggaaaacata
     2161 ctggcgaaat ttgccgaaca ggcgcaaaac aacggcagcg cgctcgccgt cggcatcagc
     2221 caatacactt cggacggcaa cggttacgaa aacgccgtca tcaacctgac cggttatcag
     2281 gaaaacaatc aggacggtat cccctactac gccaaaaacc acctcgtccc cttcggcgaa
     2341 tacaaaccgc tgcctttcct gaccacgccg ctttacaaaa tgatggatat gcccctttcc
     2401 gacttccgca aaggcggcgg caagcaatcc gccctgctga tgaaaaacca aaaaatcgcc
     2461 ttcaacatct gttacgaaga cggattcggc gacgaactga ttgccgccgc aaaagatgcc
     2521 accctgcttg ccaatgccag caatatggcg tggtacggaa aatccaacgc catgtaccag
     2581 cacctccaac aatcgcaggc gcgggctatg gaactcggac gctatatggt ccgcgccacc
     2641 aacaccggcg caaccgccat catctccccc aaaggcaaca tcatcgccca agcccaaccc
     2701 gatacggaaa ccgtattgga aggacacatc aaaggctatg tcggcgaaac gccctatatg
     2761 aaaaccggca gttcatggtg gttgatgggc atattgaccc tagccgcact gattcttttc
     2821 atcttccgaa acaaagaaca ctgatacctg cacagcccgt ccagacgggc tgtctttttt
     2881 caaaccggct gtttacatac aactacgtat gtaatataat tccgaaatcg gattgagcca
     2941 tcaggtttac aaaatcaatc aaacctgcct tacaaaattt tcctgtcttt caaaccttag
     3001 cgcatatctc ctgccaaaca tacaaaatac aagccaagca gtgatatact gcatcccaac
     3061 acagcaaacc gccgatacgc agtttcagcc gaaaggaaat gccccaaatg aataacgctt
     3121 tcgcattacc cgccatccaa agcggcaacg gcagcctcga acaatacatc cacaccgtca
     3181 acagcatccc gatgctgtcc caggaagaag aaacccgcct cgccgaacgc cggataaagg
     3241 gcgacctcaa cgccgccaaa caactcatcc tgtcccacct gcgcgtcgtc gtttccatcg
     3301 cgcgcggcta tgacggctac gggctgaatc aggcagacct gattcaggaa ggcaatatcg
     3361 gactgatgaa ggcggtcaaa cgctacgagc caggtcgagg tgcacgcctg ttttcatttg
     3421 ccgtgcattg gattaaagcg gaaattcacg aattcatcct gaggaactgg cgccttgtcc
     3481 gcgttgccac caccaaaccg caacgcaagc tgtttttcaa tttacgcagt atgcgtaaaa
     3541 acctcaacgc cctgtctccc aaagaggcac aagacatcgc cgacgatttg ggtgtcaaac
     3601 tttccgaagt tctggaaatg gaacagcgca tgacgggaca cgacatcgcc atcatggcag
     3661 acaacagcga cgacgaggac agtttcgccc ccatcgactg gcttgccgac cacgattccg
     3721 aaccgagccg acaactatcg aaacaggcgc attacgccct gcaaacagaa ggtttacaaa
     3781 acgctttggc gcaactggat gacaggagcc gccgtattgt ggaaagccgc tggcttcaag
     3841 atgacggcgg tctgacgctg caccagcttg ctgcagaata cggcgtctct gccgagcgca
     3901 tccgccagat tgaagcgaaa gccatgcaga aactgcgtgg tttcctgacc gaagaagccg
     3961 aagcggtttg aaatatacgc aataaaatac ccgacaaatt gttttgtcgg gtattttatt
     4021 tggtaaacat caatgcggtt tgtgcgtcgt gccgtacacg attttatccg tccaagccat
     4081 cgcaatcgcc gaaatcaaaa agcagacatg caccagacac tgccgcatca gctgcttttc
     4141 cgacagattg gcgaaattga tatagtggat taacaaaaat caggacaagg cggcgaagcc
     4201 gcagacagta caaatcgtac ggaaccgatt cgcttggtgc ttcagcacct tagagaatcg
     4261 ttctctttga gctaaggcga ggcaacgccg tactggtttt ggttaatcca ctatacattt
     4321 cccttccttg cagcaacaaa agcgcgtgat tccaatctgc cgctgcaaaa tcttcaattt
     4381 ttcacacccg aaaacgcttg ccgccataca gatgtgatat attgattcca ttttaacggg
     4441 cttcggagaa cgacatgagc agcggaaata tccacatcca cagcatgacc ggatttgcca
     4501 acgcggcggc agagtgcggc agcaaacgca tcaatctcga catccgcgcc gtcaaccacc
     4561 gctttttgga cattcaaatc aggatgccgg atgatttgcg ctatttggaa agcggtatcc
     4621 gcgagaaaat ttcatcccat atcgcgcgcg gcaaagtcga atgcaaaatc caaattcaag
     4681 atacggaaaa cagcagacag tccttggaac tcaactcgga tttggtgaac cagcttgccg
     4741 aaatcaacaa aaacctccgc aagcatcacg atttggcaaa a
//
LOCUS       DAWHRA010000140         4613 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWHRA010000140 DAWHRA010000000
VERSION     DAWHRA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.140"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(63..206)
                     /locus_tag="WIB30_001815"
     CDS             complement(63..206)
                     /locus_tag="WIB30_001815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797838.1"
                     /translation="MPSENLSDGIFLPKQPYFFIRAAKFYPKQQQSFHRHSRAGGNLE
                     RKI"
     gene            complement(224..1162)
                     /locus_tag="WIB30_001816"
     CDS             complement(224..1162)
                     /locus_tag="WIB30_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3797839.1"
                     /translation="MLELNGLCKRFGDKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NIIAGIVRPDGGEIWLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRNGGIPAVLVTHSPEEACTTADEIAVMHKGRILQYGTPET
                     LVKTPSCVQVARLMGLPNTDDNRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRHAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1171..2046)
                     /locus_tag="WIB30_001817"
     CDS             complement(1171..2046)
                     /locus_tag="WIB30_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ3797840.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWAERVQKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAALPETFLPENQDGEVACFEKMDIGSLVAAMLSENMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2228..2304)
                     /locus_tag="WIB30_001818"
     tRNA            complement(2228..2304)
                     /locus_tag="WIB30_001818"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2268..2270),aa:Met,seq:cat)
     regulatory      2454..2553
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2645..4546
                     /gene="thiC"
                     /locus_tag="WIB30_001819"
     CDS             2645..4546
                     /gene="thiC"
                     /locus_tag="WIB30_001819"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ3797841.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRG
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHINLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGTERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1113 a         1319 c         1213 g          968 t
ORIGIN      
        1 ttcccgcgca ggcgggaatc taggtctgtc ggcacggaaa cttatcggga aaaacggttt
       61 ctttagattt tacgttctag attcccgcct gcgcgggaat gacgatgaaa agattgttgt
      121 tgtttcggat aaaattttgc agccctgata aaaaaatatg gctgctttgg taaaaaaatg
      181 ccgtctgaaa ggttttcaga cggcattttg tttttaagaa gcatcagcgg aagcggacga
      241 tttcccgttc ttcgatatgg atgcgtaccg tatccttgcc cgataccgcc ccggcgtgcc
      301 gcatatcgag gttcagccac aggatgccgt gttccggatg gaggacggac aggctgaacg
      361 attcgggcaa acaggtacgg gataatacgc ggcactccat gccgtcttgg tcgaaacgca
      421 ccgcatgttg cggaatatgg cggttatcgt cggtattggg caaacccatc agtcgggcga
      481 cctgcacgca ggatggtgtt ttgaccaatg tttcgggcgt accgtattgt agaatcctcc
      541 ctttatgcat cacggcgatt tcgtctgccg tcgtacaggc ttcttcgggc gaatgcgtta
      601 ccaaaacggc agggatgccg ccgtttcgga tacgttcggc agtcatacgg cgcagcgtgc
      661 cgcgcaaatg cgtgtccaaa ctggaaaacg attcgtccaa cagcagcagg gaagggcgga
      721 caaccaaagc gcgcgccaac gccagccgtt gcttctcgcc tccggaaagt ttttcaggct
      781 tgcggtgcgc ctcgttttcc agtccgactt cggcaagtgc cgccatggcg aggcgttcgg
      841 cttcggcttt cggcattttt tgcattttca aaccgaatgc cgcattttcc agcgcactca
      901 tatggggaaa cagcgcgtaa tcttgaaaca tcagcgagat acggcgtttt tcgggcggca
      961 tacgggtaat gttttctccg ttcagccata tttccccgcc gtccggccgg acaatccccg
     1021 caattatatt cagcagggtg gattttccgc agcccgaccg ccccaaaacg gcgagtattt
     1081 tgccgcgccc gacagtcagg cagatgttgt cggcgacggt tttatcgccg aagcgtttgc
     1141 agagtccgtt cagttcaagc atagcgcatc ctataaacgt atgccgtcca gccactcgga
     1201 cagcggatgg gcggcatcaa tcagaccgta acggcaaaac gcgtccagcg taaccagttg
     1261 cgcgtcgtgc atcatgtttt ccgacaacat ggcggcaacc aggctgccga tgtccatttt
     1321 ctcaaaacac gcaacctcgc catcctgatt ttcaggcagg aaggtttcgg gcagagcggc
     1381 atcgaataca tacaggattt cattgtggac gccgcggctg acggggcgca ggctgtgcag
     1441 ccgggatacc ggacggataa acggaaacag cgttttatcc aaaccggctt cttcgctgct
     1501 ttcgcggcac acggcttcag acggcatttc gccgccggaa acaccgccgg cggcaatatt
     1561 gtcgagtttg tcgggatcga ctgctttgtg cggactgcgc ttgcctatcc agaaatgcca
     1621 tcggccgtcc gattcggtca gtccgttgag atggacggcg cggctgagca gtccgaacgg
     1681 acggaaagcg gcgcgttcga gcgtgaacaa ggggttgccg ccgccgtcgg tcaggtcgaa
     1741 acactcgttg cgccatccgt gaagcagccc cgccttgttc catgtgcggg cgaggtgctg
     1801 caagcggccg cccatatctg accagccgtc cgcattcaga aaaatgccgt ctgaagactc
     1861 cgagcagcct gcctcccagt ctttctgaac gcgctccgcc cattccggcg acaggtttcc
     1921 caaaggcagg ccgttcagat acagcgtttt ccagcaactt tccgcaccgt aacttgcctt
     1981 tgcccactcg aatagggcat caaggtcgtg tttgctgacg gattcggtaa aacggacggt
     2041 cggcatagtc ggctttcttg aacataatgc ggaagattgt aatcaaaccg cctgaatttg
     2101 caactgcaaa cagacggcgg gctgccgttc cgatattgcg gcaggaagga aaatccggca
     2161 aaaaacggag gcggcaggaa acaggcaaaa caaaacccct cggcattgaa ccgaggggtt
     2221 tgatatttgg ttgcgggagc aggattcgaa cctacgacct tcgggttatg agcccgacga
     2281 gctaccatgc tgctccatcc cgcgtcagaa gatgagacta tacggcagat tttttatgtt
     2341 gtcaaacatt acttccgcga aaaatcataa aattttgccg tccggcggtt tatgcctgat
     2401 ttgacatatt attttcttta caaaagttca tgttcgtgat ttaattttgg ttaacatcga
     2461 aacaggggtg ctgcctgatg ttcaggcggc tgagaaatac cctttacacc cgatcgggat
     2521 aatacctgcg tggggagttt tcacggattc tgtctttcag acggcattgg ttttcaaatg
     2581 ccgtctgaaa acgcaaaacg ctcctgtttc tttattctaa acgagaaaac aggagcattt
     2641 ttttatgact acgccaaaaa aaaccgccaa aacttccggc aacgaagcgc gcgagcttgc
     2701 cgacttgagc gaagacatcg gcatccgctt caaatatccg aactcggaac gcgtgtatct
     2761 gcaaggcagc cgtggcgaca tccgcgtgcc tttgcgcgaa atccgtcagg acgacaccta
     2821 tacggcgcaa ggtacggaag ccaatccgcc gattcccgtc tatgacacca gcggcgtgta
     2881 cggcgatccg gcggcacaca tcaatttaaa acaaggtctg ccccacatcc gcacggcgtg
     2941 gctggacgaa cgcggcgata ccgaaatcct gcccaaactc tccagcgaat acggcaccga
     3001 acgcgcacac gatccgaaaa ccgcccatct gcgtttcaac caaatcaccc gcccgcgccg
     3061 cgcgaaaagc ggcagcaacg taacccagct tcactacgcg cgccaaggca ttatcacgcc
     3121 cgaaatggag tttgtcgcca tacgcgaacg tttaaaatta gacgaattgt cccaaaaatc
     3181 ggaatacgcc aaactcttga aacaacacgc agggcaaagt ttcggcgcga acatcccaac
     3241 ccgtcccgac caaatcacgc ccgaattcgt gcgccaagaa atcgccgccg gacgcgcgat
     3301 tatccccgcc aacatcaacc accccgaact cgaaccgatg attatcggcc gcaacttccg
     3361 tgtcaaaatc aacggcaact tgggcaattc cgccgtcact tccagcctga ccgaagaagt
     3421 cgaaaaaatg gtgtggtcgc tgcgttgggg cgcggacaca attatggact tatccacagg
     3481 cgcgcacatc catgaaacgc gcgaatggat tatccgcaac gcgcccgtcc ccatcggcac
     3541 cgtgcccatc tatcaggctt tggaaaaaac cggcggcatt gccgaagatt tgacttggga
     3601 tttgttccgc gacaccttaa tcgagcaggc ggagcaaggc gtggactatt tcaccataca
     3661 cgcgggcgtg ttgctgcgtt atgtgccgat gaccgccaac cgcctcaccg gcatcgtctc
     3721 gcgcggcggt tcgattatgg cgaaatggtg tttggcacat catcgggaaa atttcctcta
     3781 cacgcatttc gacgaaatct gcgaaattat gaaagcgtat gacgtatcgt tcagcctcgg
     3841 cgacggcctg cgccccggct gcattgccga tgccaacgac gaatcccaat tcgccgaact
     3901 gcacaccttg ggcgaattga ccgataaagc gtggaaacat gacgtacaag tcatgatcga
     3961 aggccccggc catgtgccgc tgcaacgcgt caaagaaaac atgaccgaag agctgcaaca
     4021 ctgctttgaa gcaccttttt acacgctcgg cccgctcgtt accgacatcg cacccggcta
     4081 cgaccacatc acctcgggca taggcgcggc caatatcggc tggtacggca cggcgatgct
     4141 ttgttacgtt acccccaaag aacatttggg gctgcccgac aaagaagacg tgcgtaccgg
     4201 catcatcacc tacaaactcg ccgcccacgc cgccgatctc gccaaaggct ggccgggcgc
     4261 acaattacgt gacaacgccc tgagcaaagc gcgtttcgag ttccgctggc gcgaccaatt
     4321 ccgcctcagc ctcgaccctg aacgtgccga gagcttccac gacgaaaccc tgcctgccga
     4381 aggcgcgaaa atcgcccact tctgctcaat gtgcggcccc aaattctgct cgatgaaaat
     4441 cacgcaggaa gtgcgcgact acgccgacaa gcaaaaagcc caacggcagg gcatggagga
     4501 aaaagcggtc gagttcgtca aaaaaggcgc gaagatttac agttgaaacg tcaagcaaaa
     4561 aatgccgtct gaaacccgga aaaaaggctt cagacggtat tctttcgctt gtg
//
LOCUS       DAWHRA010000141         4445 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWHRA010000141 DAWHRA010000000
VERSION     DAWHRA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.141"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(63..1205)
                     /locus_tag="WIB30_001820"
     CDS             complement(63..1205)
                     /locus_tag="WIB30_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ3797842.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLHLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLLAGYDGTQPFQDPFCGSGTIAVEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWTDLRRRAEAQIRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTSDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVEGSNR"
     gene            complement(1254..1676)
                     /locus_tag="WIB30_001821"
     CDS             complement(1254..1676)
                     /locus_tag="WIB30_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797843.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPVGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="WIB30_001822"
     CDS             complement(1669..2478)
                     /locus_tag="WIB30_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ3797844.1"
                     /translation="MIQDTRPLIRVVAGILLNRDGDYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHAHVCLKFLWVNPDQWTGEPQ
                     SREGQAWSWQKAGDFTIAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAEESGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /locus_tag="WIB30_001823"
     CDS             complement(2560..2937)
                     /locus_tag="WIB30_001823"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ3797845.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="WIB30_001824"
     CDS             complement(3137..3904)
                     /locus_tag="WIB30_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ3797846.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..>4445)
                     /locus_tag="WIB30_001825"
     CDS             complement(3914..>4445)
                     /locus_tag="WIB30_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ3797847.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPTSFDKKQGVIWGNILSLNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1087 a         1101 c         1091 g         1166 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcttttt tgtacacttt
       61 cctcaacggt tcgatccttc caccatatca atcaggaaca ggcggcaatc gaggttgccg
      121 ttataaagcg ggattttgcg ttttggcgac aggcgcatga atttgggcat ttccctgtcg
      181 ctggtaaaca tgccgacccg ccagccggca tagtgttttt tcaaccacgt ccccagctgc
      241 ggataaagag cctgcaaagc gcggacttcc tcaaggcgca caccgtaggg cggattggac
      301 accatgatgc cgttttcgcc gttcggacgg acggattgcg cgtcggcaac gctgaaggaa
      361 acgatgtcgt ccacttcggc actgcgtgcg ttgtccaatg ccgtctgaac gatgcggcgg
      421 tcgctgtcgc tgcctgcaat cggggcgcgg acggggcgga tttgcgcttc ggcgcggcgg
      481 cgcaaatctg tccacagcgt tttatcgaaa ttttgcagtt tctcaaaacc gaaacggcgc
      541 atcatacccg gcgcgcggcc tgtggcaatc caagcggcct cgacggcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccaagag cagcagtccg
      661 gcagcaaggt tttcgcgcag cggggcttcg ccggtatcca ggcggtagcc gcgtttaaac
      721 agggcttcgc ccgaagtgtc gataaagatt tcgacattgc gttcgttcaa aaaggcgtgg
      781 atgcggacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaaa
      841 gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt ggatgctctt aacgttggca
      901 cgctttgcct cgactttgac tttgaacgtc tgctgcaagg taaaccagtt gaaccagttg
      961 atgtttttgg caagtttgta aatgtcgcgc tcggttcggt atgtgccttt ggtcaggtgc
     1021 agcaggatac ggccggcggt gcgcgagtgc aggttggcgg cgtaggcctg ttccaatccg
     1081 ccgcggcagg aaacgccgcc gtcaaacgct tgaacatcgg tacagccgag gcttccgagt
     1141 tcttgagata aaacggtttc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcataacgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccggctg ccgattgtaa tgtctgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgttccg tgccgacggg cgcgtgttcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cggcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacggata cgccctgccg
     1801 cagggcttct gccgcctctt gtgcctgaac atcatcgcac acgcgccaaa cgaccgcatc
     1861 cgcgcccttt tcctgcgcct gccgccattg ttcgcgggtc tgcaccatca tccacacgct
     1921 gtcggcgtgt tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 ttcctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgtttt ctccgtacaa
     2041 acctgttttc aggctgccgt acaaacggcg cggaacggac aacgaacgca aaagcgcgcc
     2101 gttggcgggc agcatggggg caatggtaaa atcacccgcc ttctgccacg accatgcctg
     2161 tccttcgcgc gattgcggtt cgcccgtcca ttggtcgggg tttacccata ggaatttcag
     2221 gcagacatgc gcgtgttcgt aggaatggat tttggtcagc caaggcgtgg cggcgaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcaaggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttacctt cggggcggga
     2401 actgagcagg tagtcgccgt ctcggttgag caggatgccg gcaacgacgc ggataagggg
     2461 tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggacg
     2521 ggcaatgccg tcaacgggtt tgccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 aaaggcttct ttggcggcaa agcgtttggc aaggtagttg acgggtttgc ccgcctgagg
     2821 aaattcaagc agctcttccg gagtgaggat gcgcccggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca ctttcggggt tttgtcggta acaattttta
     3061 ttgtaacctt ttgcatttga aattctaaaa catcgtgcca attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcacccccaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccctttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ataaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttct ggcatgcaag ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataacatt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat acctttatag atacaatcac
     4141 ttctaaatct aatcatttgt gtattgagcg ataagatatt accccatatc acaccttgtt
     4201 ttttatcaaa tgatgttggt gttttaaatg gatataaatt gcccgttaat gtttttaagt
     4261 gttttttacc ggtttctgtt ttagataact gattgacaat atctgatgaa cttaaaaata
     4321 gggaattacc tccatcattg gctgatttta ctacatacat aaccaaataa ctttccgggt
     4381 tttcactaaa agatgaatca ctatgaagtg gacattctcc aacatcttcc gagaaagtta
     4441 tatca
//
LOCUS       DAWHRA010000142         4438 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWHRA010000142 DAWHRA010000000
VERSION     DAWHRA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.142"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            57..480
                     /locus_tag="WIB30_001826"
                     /pseudo
     CDS             57..480
                     /locus_tag="WIB30_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            586..1149
                     /gene="pgsA"
                     /locus_tag="WIB30_001827"
     CDS             586..1149
                     /gene="pgsA"
                     /locus_tag="WIB30_001827"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ3797848.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1229..1304
                     /locus_tag="WIB30_001828"
     tRNA            1229..1304
                     /locus_tag="WIB30_001828"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1262..1264,aa:Gly,seq:gcc)
     gene            1330..1405
                     /locus_tag="WIB30_001829"
     tRNA            1330..1405
                     /locus_tag="WIB30_001829"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1363..1365,aa:Gly,seq:gcc)
     gene            1429..1504
                     /locus_tag="WIB30_001830"
     tRNA            1429..1504
                     /locus_tag="WIB30_001830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1462..1464,aa:Gly,seq:gcc)
     gene            1526..1601
                     /locus_tag="WIB30_001831"
     tRNA            1526..1601
                     /locus_tag="WIB30_001831"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1559..1561,aa:Gly,seq:gcc)
     gene            1627..1700
                     /locus_tag="WIB30_001832"
     tRNA            1627..1700
                     /locus_tag="WIB30_001832"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1659..1661,aa:Cys,seq:gca)
     gene            1803..1892
                     /locus_tag="WIB30_001833"
     tRNA            1803..1892
                     /locus_tag="WIB30_001833"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1837..1839,aa:Leu,seq:taa)
     gene            1996..3048
                     /gene="sohB"
                     /locus_tag="WIB30_001834"
     CDS             1996..3048
                     /gene="sohB"
                     /locus_tag="WIB30_001834"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ3797849.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3099..3965)
                     /locus_tag="WIB30_001835"
     CDS             complement(3099..3965)
                     /locus_tag="WIB30_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ3797850.1"
                     /translation="MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGRAINAV
                     IAGEVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSRKFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLKNSLERDNHFIRKGDRRQLYRHYGLLARLRVLISNREAFGYLCVG
                     TAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IGGLERNIKAGT"
     gene            complement(3976..4407)
                     /locus_tag="WIB30_001836"
     CDS             complement(3976..4407)
                     /locus_tag="WIB30_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ3797851.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRKRLLSLTETGKAYAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1097 a         1050 c         1160 g         1131 t
ORIGIN      
        1 ttgttaatcc actatattat cgctgggtat ggtttgccgc cataagctgt ctgctgttgt
       61 ccgccgtttt tattgcacct tacctgacgg catttcacga acaagaaaaa acattagaat
      121 atgcggaatt gaccgttacc gcccccaatc gcagcggacg ggcaatcaaa ctggatgcag
      181 acgggcagca ttaccggctt tcctgctatg ggttcgacag tttgtgcaca ggcggcaata
      241 tcggcagaac catcagggcg gagcaggtta gagccgtttt aagcgaaacc gtcggcaaag
      301 gttttttaaa cggcgttctg ttggaatacc gcaacagtgg cagtgtctat aagcaataaa
      361 gattttgccc gcacggaaga ccgccttgtc gaagtgttgg cacaacctgc cgttttcagc
      421 ctgaaactgg gtattttgct tttattgccc gccattttcc tgcgtttgaa aagaatgtga
      481 aaacaaattg gcggggcggg ggaatgccca tgcggcaatt ttacgttttg ggttatgagg
      541 ataaccgtta taatcacaac tttacagtct gcacggagaa aaccgatgcc ttggaatctc
      601 cccatcttcc tcacatggtt gagggtcttg ctcattcctg tcatgacagc ccttttttac
      661 ctgccttttc cgtggttcgc ggaggaaacg attaatctcg ccgccgccgt tatttttgcc
      721 gttgccgcct taaccgactg gtttgacgga tttttggcaa gactgtggaa acagacttcc
      781 gatttcggcg ccttcctcga tcctgtcgcc gacaagctga tggttgccgt atcgctactt
      841 ttactggtca aactcgaccg gacctatgtt ttgtttgcca tgatcatcat cggcagggaa
      901 attaccattt ccgcattgcg cgaatggatg gcgcaaatgg gcaaaaggaa cagcgttgcc
      961 gtcgccaccg tcggtaagtt taaaaccgcc gcgcaaatgc tggcaatttt cctgctgctg
     1021 ctgaatattc ccgattttta cggatttaat cttgttgtta tcggcaacat attgatgttt
     1081 atcgcatctt tgctgacggt ttggtcgatg ctgtattatc tgaaaatggc gtggaaagaa
     1141 atcgcctgaa aaaaacataa aaatagcttg acggcaaaaa cagaatccat aataattgcg
     1201 tctcttcgat gttggagaat gaaatcgggc gggaatagct cagttggtag agcgcaacct
     1261 tgccaaggtt gaggtcgcga gttcgagact cgtttcccgc tccaaaaatt tcattttccc
     1321 acaatcattg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1381 agttcgagac tcgtttcccg ctccaagttt tatttcaaac atatcaacgc gggaatagct
     1441 cagttggtag agcgcaacct tgccaaggtt gaggtcgcga gttcgagact cgtttcccgc
     1501 tccaaaaacc gatatgtttg aacgcgcggg aatagctcag ttggtagagc gcaaccttgc
     1561 caaggttgag gtcgcgagtt cgagactcgt ttcccgctcc aagtttttta gataagccag
     1621 ttttagggcg agatagcaaa gtggttatgc agcggattgc aaatccgtct acgccggttc
     1681 gattccgact ctcgcctcca ttatcgtact atggcggggt ggcagagtgt ttatgctatg
     1741 aggagtgcaa tcttcataca ggccggttaa aatccgcgcc cccgcctcca cctttcacaa
     1801 atgcccgggt ggtgaaatag gtagacacaa cggacttaaa atccgtcggg actaaacatc
     1861 ccgtgccggt tcgattccgg ctccgggcac caagctgaaa atgacaatgc cgctccaagc
     1921 ggttattttt ttatctgtcg gacggtgatg gacaatattg acatgttcat gcctgaacaa
     1981 gaggaaatcc aatcaatgtg gaaagaaatt ttactgaatt acggtatttt cctgctcgaa
     2041 ctgcttaccg tgttcggcgc aattgcgctg attgtgttgg ctatcgtaca gagtaagaaa
     2101 cagtcggaaa gcggcagtgt cgtactgacg gatttttcgg aaaattataa aaaacagcgg
     2161 caatcgtttg aagcattctt tttaagcggg gaagaggcaa aacatcagga aaaagaggaa
     2221 aagaaaaagg aaaaggcgga agccaaagca gagaaaaagc gtttgaagga gggtggggag
     2281 aaatctgccg aaacgcaaaa atcccgcctt tttgtgttgg attttgacgg cgatttgtat
     2341 gcacacgccg tagaatcctt gcgtcatgag attacggcgg tgcttttgat tgccaagcct
     2401 gaagatgagg ttctgcttag attggaaagt ccgggcggcg tggttcacgg ttacggtttg
     2461 gcggcttcac agcttaggcg tttgcgcgaa cgcaatattc cgctgaccgt cgccgtcgat
     2521 aaggtggcag cgagcggtgg ttatatgatg gcgtgtgtgg cggataaaat tgtttccgct
     2581 ccgtttgcga ttgtcggttc ggtgggtgtg gtggcggaag taccgaatat ccaccgcctg
     2641 ttgaaaaaac atgatattga tgtggatgtg atgacggcgg gcgaatttaa gcgcacggtt
     2701 acttttatgg gtgaaaatac ggaaaagggc aaacagaaat tccgacagga actggaggaa
     2761 acgcatcagt tgttcaagca gtttgtcagc gagaaccgcc ctcaattgga tattgaggaa
     2821 gtggcaacgg gcgagcattg gttcggtcgg caggcgttgg cgttgaactt gattgacgag
     2881 atttcgacca gtgatgattt gttgttgaaa gcgtttgaaa acaaacaggt tatcgaagtg
     2941 aaatatcagg agaagcaaag cctgatccag cgcattggtt tgcaggcgga agcttctgtt
     3001 gaaaagttgt ttgccaaact tgtcaaccgg cgggcggatg tgatgtagtt tgccgaacgg
     3061 atggaaatgc cgtctgaagc gtgttcagac ggcatttttc aagttccggc tttgatgttc
     3121 cgttccaatc cccctatccg ttgtccgatg tctttcaaat tggaatattg ttcgaccaat
     3181 cgcggcacgt cgtccaaact catggcaaac atccacagat aagtgccgac cgaatagaca
     3241 tgccccgcgc tgctgtagcc tttgagcgtc atcatcacaa aagcaaagcc gaacaaaata
     3301 cccatcgccg tgccgacgca gagatagccg aaggcttcgc ggttggaaat cagcacacgc
     3361 aggcgcgcaa gcagtccgta atggcggtac agctgccgcc ggtcgccttt tcggataaag
     3421 tggttgtcgc gttccaagct gttcttcagg cggaaataca ggttttcgct gatggcggca
     3481 aaacgtggca aaagccataa aaacaacgca agtatgccca ccgccgacac gccgacccaa
     3541 aattccagca ccagcagcat gatgcacgca ccgaatatgg atacgacgga tgtcgcggca
     3601 atcggcaggt gttcttcaaa aaagctgaca aacttacgtg acagggcaac ccgtgcagtt
     3661 accgccgaat gcgggacttg tcgctgccgc tgttccaaca caaccggcac ggcgatttcg
     3721 gtataaatcc gcgtaaacgt gcgcgtatcg gcaatccgcc gcgccgcacc gaccagccac
     3781 attaaaagca caaccaaagc gtacagcaac gcctgccaca cctcccccgc aatcacggca
     3841 ttgatcgccc ggccgccaaa caccggatac accagcatca aaaggttttc cagtccgacc
     3901 agggaaaatg tgccaatcaa tcgcttgcgg tgggtttggg ctatgtgttt caacattttc
     3961 cacatatttg cccccctatt ttttattttc cgagattgtt ttttccatca cttcagccag
     4021 tgcatccaaa tcggcaaaca gccgagttgt gcgcttgtcg ccgaatgtgg caaatacttt
     4081 gtcgctgaat tcctgcgcgc tttctgttaa aggtgcggca taggctttgc ctgtttcggt
     4141 caacgacagc aaccgtttgc gccggtcctg ttcgccttcc tgccattcaa tcaacccttg
     4201 tccggcaagg gttttgcata cgcctgaaac ggtctgtttg ggcaggctcc acttttcgcc
     4261 gatatgcttt tgcgtgcggc tgccttcggt tgccagggta taaagtacgg caaagaggtt
     4321 gtaattcaga tcctgctgcc cgatccattt gtcgaagaca ttgcaaatca ggttgatacg
     4381 ggtgccaagt tggtcgagtt ggttcataat tggtcttgat attgactaaa tttgtgcg
//
LOCUS       DAWHRA010000143         4335 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWHRA010000143 DAWHRA010000000
VERSION     DAWHRA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.143"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            67..606
                     /locus_tag="WIB30_001837"
     CDS             67..606
                     /locus_tag="WIB30_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ3797852.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(796..1011)
                     /locus_tag="WIB30_001838"
     CDS             complement(796..1011)
                     /locus_tag="WIB30_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797853.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(1019..1222)
                     /locus_tag="WIB30_001839"
     CDS             complement(1019..1222)
                     /locus_tag="WIB30_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797854.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(1135..1494)
                     /locus_tag="WIB30_001840"
     CDS             complement(1135..1494)
                     /locus_tag="WIB30_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ3797855.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            1547..1894
                     /locus_tag="WIB30_001841"
     CDS             1547..1894
                     /locus_tag="WIB30_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3797856.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVLVPF"
     gene            2122..2742
                     /locus_tag="WIB30_001842"
     CDS             2122..2742
                     /locus_tag="WIB30_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ3797857.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            2765..4216
                     /locus_tag="WIB30_001843"
     CDS             2765..4216
                     /locus_tag="WIB30_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ3797858.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     IGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT         1143 a          905 c          951 g         1336 t
ORIGIN      
        1 acggtaaaag ccgggtatag atacctaacc cttgtttata gctgaaaatc aataaggaag
       61 aacattatgt cccaagtttt taaagatttt gatttgtcct ccgtatggga atccgacagt
      121 tgggcagatg aaaactacaa agaagccccg tttacccctg aaattttggc tgccgtagaa
      181 agtgaactgg gctataaatt gccgcaaagt tttattgaat tgatggcggt acaaaacggc
      241 ggaatatttg tcaaaaactg ttttccgacc acacagagaa attcgtgggc gaaagatcat
      301 gtgcaaattt gcgaggtatc gggcatcggc tttgaaaaag aagggagttt gtgcggcgaa
      361 acagggcaaa aactttggat ggaagaatgg gaatatccgc ccatcggcgt gtattttgcc
      421 aacgacccgt caggcggtca tgccatgttt gccttggact atcgggagtg tggaaaagac
      481 ggcgagccga aagtggtgtt tgtcgagcaa gaatcggatt ttgaaatcgt cgaacttgcc
      541 cccgattttg aaacctttat ccgcagcttg cggcatgaag atgagtttat tgacgaggaa
      601 atataaaacg gcggttgaaa aaccggaatc atcaagggaa aacgggcgaa ataacggata
      661 atcgcttgaa tccgtaagga aaacggtttg gtggaacgcg ccatccaaga cctttgcaaa
      721 gaaaaactgt ctccgacagc attgacatta ttaacagaac ttatcaagtt tggagctatc
      781 tcaaatataa ttcggttatc cttttgtatc cattaaatca tatgcttcaa ttaattgttg
      841 ttctagctct tgtaccaatt ttgaattgcg aattcctgac acaatctcaa attcttctgc
      901 atctatgcaa acacctgcat aaatttcaat aacaagggaa cgcaataatt gaagctcttc
      961 tcttgttaaa gaaataataa tgtcatcacc tgtgtaattg attatattca taataatttt
     1021 atttttgttt gtcaaagtaa gttttgccta aggttggtct aaatgcagtt ccaccatctt
     1081 ttgaatttgg gtctctgatt acaattgctc cagacttatc atcccaaatt gctcttatgt
     1141 gtttggattg taatcttcga attcccaaga aaaaaatcgt aataagtttg aaagtgtcaa
     1201 atcccaagtt tcttttgagc aatattctaa tattttatca atttcacttt taataatctt
     1261 atgatcaaac tgttctaata ttaatgcatt agaccaaaaa aaaccttctt tattacaatg
     1321 atgggaaatc catttaggag aacaaatgca aagtgaaaaa atagatgagc cttgttctcc
     1381 ttcgattccg atatccaaat ctatccatct atggaaatta tctggaattt cgggggtaaa
     1441 tttttcaaaa tcaatatcat ataaatttat gctttttaaa tccaatttaa tcattagggc
     1501 tgtcctagat aaatagggaa attcaaatta agttagaatt atccctatga gaaaaagtcg
     1561 tctaagccgg tataaacaaa ataaactcat tgagctattt gtcgcaggtg taactgcaag
     1621 aacagcaaca gagcctgaca gcattgttta tacggattgt tatcgtagct atgatgtatt
     1681 agatgtgagc gaatttagcc atttccgtat caatcacagc acacattttg ctgaacgaca
     1741 aaaccatatc aatggaattg agaatttttg gaaccgggca aaacgtcatt tacgcaagtt
     1801 taacggcatt cccaaagcgc attttgagct gtatttaaag gagtgcgaat ggcgttttaa
     1861 caacagtgag ataaaagttc ttgttccatt ttaaaacaat tagtaaaatc gagtttatct
     1921 tagttgtcca ggacagcccc attattttta taacaccgtg aagccgcaca gcagtttgaa
     1981 cagtgatacg ccgtttgcgg gcttacgagt ttattttccc ggcctgcagt ttgagcaata
     2041 cggtgatttc ctacggttaa tacaaatgtt tacacattga tacatttcat ttatagttcc
     2101 gcctatttga aaatagaaaa tatgaattcg accgcaagta aaaccctgaa aggattgtcg
     2161 ctggtgtttt tcgcctctgg attctgcgcc ctgatttacc aggtcagctg gcagaggctt
     2221 ctattcagtc acataggtat cgatttgagt tcgattactg tcattatttc tgtatttatg
     2281 gtcggcttgg gtgtaggtgc gtatttcggt ggacgcattg ctgaccgttt tccttcaagt
     2341 atcatccccc tgttttgcat cgctgaagta tccatcggtc tgttcggttt ggtaagcagg
     2401 ggtctgattt ccggcttggg gcatctttta gttgaggctg atttgcccat catcgctgct
     2461 gccaatttcc tcttattgct gcttcctacc tttatgatgg gcgcgacctt gcccttgctg
     2521 acctgttttt ttaaccggaa aatacataat gttggcgagt ctatcggtac cttatatttt
     2581 ttcaacactt tgggtgcggc actcggatcg cttgccgccg ccgaattttt ctacgtcttt
     2641 tttaccctct cccaaaccat tgcgctgaca gcctgcttta accttctgat tgctgcttca
     2701 gtatggctgc gttacagaaa ggatggatat agtgaacact aaaccgaata ctagtttaat
     2761 ttatatgctt tctttcctta gcggcttatt gagcttgggt atagaagtct tgtgggtaag
     2821 gatgttttcg ttcgcagcac agtccgtgcc tcaggcattt tcatttactc ttgcctgttt
     2881 tctgaccggt atcgccatcg gcgcgtattt tggcaaacgg atttgccgca gccgctttgt
     2941 tgatattccc tttatcgggc agtgcttctt gtgggcgggt attgccgact ttttgatttt
     3001 gggtgctgcg tggttgttga cgggtttttc cggcttcgtc caccacgccg gtatcttcat
     3061 taccctgtct gccgtcgtca gagggttgat tttcccgctc gtacaccatg tgggtacgga
     3121 tggcaacaaa tccggacgac aggtttccaa tgtttatttc gccaacgttg ccggcagtgc
     3181 attgggtccg gtccttatcg gctttgtgat acttgatttc ttgtccaccc aacagattta
     3241 cctgctcatc tgtttgattt ctgctgctgt ccctttgttt tgtacactgt tccaaaaaag
     3301 tctccgactg aatgcagtgt cggtagcagt ttccctaatg ttcggcatcc tcatgttcct
     3361 actgccggat tctgtctttc aaaatattgc tgaccgtccg gataggctga ttgaaaacaa
     3421 acacggcatt gttgcggttt accatagaga tggtgataag gttgtttatg gggcgaatgt
     3481 atacgacggc gcatacaata ccgatgtatt caatagtgtc aacggcatcg aacgtgccta
     3541 tctgctaccc tccctgaagt ctggcatacg ccgcattttc gtcgttggat tgagtacagg
     3601 ttcgtgggcg cgcgtcttgt ctgccattcc ggaaatgcag tcgatgattg ttgcggaaat
     3661 caatccggca taccgtagcc ttatcgcgga cgagccgcaa atcgccccgc ttttgcagga
     3721 caaacgtgtt gaaattgtat tggatgacgg taggaaatgg ctgcgtcgcc atcctgatga
     3781 aaaattcgac ctgattttga tgaatacgac ttggtactgg cgtgcctatt ccaccaacct
     3841 gttgagtgcg gaatttttaa aacaggtgca aagccacctt accccggatg gtattgtaat
     3901 gtttaatacc acgcacagcc cgcatgcttt tgctaccgcc gtacacagta ttccctatgc
     3961 ataccgctat gggcatatgg tagtcggctc ggcaaccccg gtagttttcc ctaataaaga
     4021 actgctcaag caacgtctct cccggttgat ttggccggaa agcggcaggc acgtatttga
     4081 cagcagcacc gtggatgctg cagcacaaaa ggttgtctct cgtatgctga ttcagatgac
     4141 ggaaccttcg gctggggcgg aagtcattac cgacgataat atgattgtag aatacaaata
     4201 cggcagaggg atttaaccgt cttaaagggt ttcaggcaac gcaggtttta ggtaacgtcc
     4261 tgctagttca aaaaaaccgc atcacagcag tcgggacaaa atggtttaaa cattttgtcc
     4321 cgaattctta ttcct
//
LOCUS       DAWHRA010000144         4222 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWHRA010000144 DAWHRA010000000
VERSION     DAWHRA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.144"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(84..1130)
                     /gene="recA"
                     /locus_tag="WIB30_001844"
     CDS             complement(84..1130)
                     /gene="recA"
                     /locus_tag="WIB30_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ3797859.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1384..2148
                     /locus_tag="WIB30_001845"
     CDS             1384..2148
                     /locus_tag="WIB30_001845"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ3797860.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGAFPCSDDYYFKLL
                     DALIESRLPDIIDIELFAGETAVRRAVADAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPVVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            2168..4183
                     /gene="rep"
                     /locus_tag="WIB30_001846"
     CDS             2168..4183
                     /gene="rep"
                     /locus_tag="WIB30_001846"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ3797861.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRHKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHKHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT          993 a         1156 c         1166 g          907 t
ORIGIN      
        1 caaaactcaa atcaagccgt tcggaggcgg ctcaaaaaaa cggtacttcg cagcagaagt
       61 accgtttatc gggatttcag gttttattct tcggggcgtt cgccgtcggt ttcgtcctgc
      121 gtcccttcgg tgatgtgcat ttctacgccg ttgagggcgc ggatttttgc gtcgatttca
      181 ttggcgactt cgggattttc cttcagccag acgcggacgt tgtctttgcc ctgaccgatt
      241 ttcgcgccgt tgtagctgta ccacgcgccg gatttgttga tgatgtcgtt tttcacgccg
      301 atgtcgatca attcgccttc ccaactgatg ccttctccgt agaggatgtc aaactctgcc
      361 tgacggaacg ggggggcgac tttgtttttg atgactttga cgcgggtttc gttgcccaat
      421 acttcttcgc cttttttgat ggatccggtg cggcggatgt cgaggcggac ggaagaatag
      481 aatttcagcg cgttgccgcc ggtggtggtc tcggggctgc cgaacattac gccgatcttc
      541 atccggattt ggttgatgaa cacaaccagc gtgttggttt ttttgatgtg tccggtcagt
      601 ttgcgcaaag cctgactcat caggcgcgcc tgcagtccga catggctgtc ccccatatcg
      661 ccttcgattt cggctttggg gacgagtgcg gctacggaat cgacgactac catatctatg
      721 ccgcccgaac ggacgagtgt gtcgcagatt tccaaagcct gttcgccggt atcgggctgg
      781 gacaggtaaa gctcttcgac ttttacgccg agtttgcggg cgtaaacggg atcaaaggcg
      841 tgttcggcat cgacaaaggc gcacacgccg ccgtttttct ggcattgggc gacggcttcg
      901 aggcagaggg tggttttgcc ggaggattcg gggccgaaga tttcgacgat gcgcccgcgc
      961 ggcagaccgc cgactccgag ggcgaggtct aatccgagcg atccggtgga aatgacttcg
     1021 aggttttctt cctgctggct gccgtccatt ttcatgatgg cgcctttgcc gaaacttttt
     1081 tcgatttgcg ccagtgcggc ggcaagggct ttgcttttgt cgtctgacat tggggttact
     1141 ccggaacaaa tgcggtatgt aggatgcggc gcaacacggg ctgcggcgcg ggatgtgtat
     1201 cgttttcccg atgtgcgggc tatcggtaat gctgcttcac gaggttgcca ttatcgcata
     1261 tttccttgct tgccgatatg cggcaggacg cggcggcttg tgccggaatg gaatctggat
     1321 gccgtctaaa aggcggccgg ctttgttata atggcggctg ttttttctgt gtgtgcctgt
     1381 tttatgtgtt cctgccttgt tgtcaaaaat accgttatcg gaagcggacg caccaaaatc
     1441 gccgtgccgc ttgtcgcccg cgatgccgcc gaactttccg ccgtacttga ccaaatcaaa
     1501 aacctgccct tcgacatcgt cgagttccgc gccgattttt tggaatgcgc gggcagcatc
     1561 ggcgaagtat tgcgccacac gcagaccgtc cgcgacgcgc tgcccgacaa gccgctgctg
     1621 tttacgttca gacggcatgg cgaaggcggc gcgttcccgt gttcggacga ttattatttc
     1681 aaactgctcg acgcgctgat cgaaagccgc ctgcccgaca tcatcgacat cgagctgttt
     1741 gccggcgaaa ccgccgtccg gcgcgccgtg gcggatgctc aaaaaaacgg catcgccgcc
     1801 ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag aagaaatcgt atgccgtctg
     1861 aaacagatgg aggactgcgg cgcggacatc tgcaaaattg cggtgatgcc gcaaagcgcg
     1921 gaagatgtgc tgactttgct ttccgccacg ctcgaagcga aacggcttgc cgccaaaccg
     1981 gttgttacga tgtcgatggg gcagacgggt gcggtcagcc gccttgccgg acaggtgttc
     2041 ggctcaagca tcacgttcgg ttcgggaacg caaaactccg cgccggggca aatcggcgta
     2101 tccgccctcc gtgcggcact cgactgtctc gaaagcggcg cagactgatt tcagacggca
     2161 tcaaaacatg atgaaactca atccccaaca gctcgaagcc gtccgttacc tcggcggtcc
     2221 cctgctcgtc ctcgccggcg ctggcagcgg caaaaccggc gtgattacgc aaaaaatcaa
     2281 gtatttgatt gtcaatgtcg gctatctgcc gcataccgtt gccgcgatta cctttaccaa
     2341 taaagccgcc gcggaaatgc aggagcgcgt cgccaaaatg ctgcccaagt cgcaaacgcg
     2401 cgggctgacg atttgcacgt tccattcttt gggcatgaag attttgcgcg aagaggcgaa
     2461 ccatatcggt tacaaaaaaa acttctccat tctcgattct accgacagcg cgaaaatcat
     2521 cggcgaactc ttaggcggta cgggcaaaga agccgtattc aaggcgcagc accagatttc
     2581 cttgtggaaa aacgatttaa aaacgcctga agatgtcgtt cagacggcat cgaacatttg
     2641 ggaacaacaa accgcacgcg tgtatgcgag ctatcaggaa accttacaaa gctatcaggc
     2701 agtggacttt gacgacttaa tccgcctgcc tgccgtgctg ttgcagcaaa acagcgaagt
     2761 gcgccacaaa tggcagcggc ggctgcgtta tctgctggtt gacgaatgcc aagatacgaa
     2821 tacctgccaa tttacgttga tgaaacttct gaccggcgcg gaaggtatgt ttaccgccgt
     2881 cggcgacgac gaccagtcca tctacgcatg gcgcggtgcg aacatggaaa acctgcgtaa
     2941 aatgcaggaa aactatccgc agatgaaggt catcaaactg gagcaaaact accgctccac
     3001 cgcgcggatt ctcaaaatcg ccaacaaagt catcgaaaac aaccccaagc tgtttaccaa
     3061 aaaactttgg tcgcaattgg gcgaaggcga gccggtcaaa gtcgttgcct gccaaaacga
     3121 gcaacacgaa gccgactggg tcgtcagcca aatcgtcaaa caaaaactca tcggcggcga
     3181 caaaacccaa tatgccgatt tcgccgtgtt gtaccgggga aagcatcagg cgaggatttt
     3241 cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc tccggcggac aaagcttttt
     3301 cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg ctgcttgcca accccaacga
     3361 cgatcccgcc tttctgcgtg ccgttaccac gcccaaacgc ggcatcggcg atgtaacgct
     3421 gggcaagctc aacacttacg cgcacaaaca cgaatgcagc ctgtatgaag ccgcgcaaaa
     3481 cgaagaagcc ctcgccacgc tgaacaatac caaccgccaa cacctgcaag cctttatgga
     3541 tatgttcggc agttaccgtg ccaaagccga aaccggcgaa gcgggcgagt tcatcaacag
     3601 cctgctcgaa gaaatcgact atgaaaacca cctgcttgcc agtgaagaag gcaaagctgg
     3661 cgaaatcaaa tggcgcaacg tcggcgattt ggtatcatgg tttgcgcgaa aaggcgggga
     3721 agacggcaaa aacatcatcg aactcgccca aaccgtcgcc ttgatgacgc ttttggaagg
     3781 aaaagacgaa gaagaaaccg atgccgtctc gctatccacg ctacacgccg ccaaaggttt
     3841 ggagtatccg tatgttttcc ttgtcggttg cgaagaaggc gttttgccgc acaacgacag
     3901 catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg tacgtcggca tcacccgcgc
     3961 caaacgccaa ctcacactga cccactgcgt caaacgcaaa aaacaaggca catggcagtt
     4021 ccccgaaccc agccgattca tagacgaaat gccgcaggaa gatttgaaaa tcctgggtcg
     4081 caaaggcggc gaaccgattg tcagcaaaga agaaggcaga cgcaaccttg ccgatataat
     4141 cggaaggctc gacagcatga aaaaaggcgg tccggaggat taaaccaaga tagggaaacg
     4201 aagccggaga caatgtatag tg
//
LOCUS       DAWHRA010000145         4202 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWHRA010000145 DAWHRA010000000
VERSION     DAWHRA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.145"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1..143)
                     /locus_tag="WIB30_001847"
                     /pseudo
     CDS             complement(1..143)
                     /locus_tag="WIB30_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(271..1521)
                     /locus_tag="WIB30_001848"
     CDS             complement(271..1521)
                     /locus_tag="WIB30_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ3797862.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVRKQVTALCNKYPVYGA"
     gene            complement(1588..1783)
                     /locus_tag="WIB30_001849"
                     /pseudo
     CDS             complement(1588..1783)
                     /locus_tag="WIB30_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2002..3777)
                     /gene="ggt"
                     /locus_tag="WIB30_001850"
     CDS             complement(2002..3777)
                     /gene="ggt"
                     /locus_tag="WIB30_001850"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ3797863.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3945..4181)
                     /locus_tag="WIB30_001851"
     CDS             complement(3945..4181)
                     /locus_tag="WIB30_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ3797864.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENPEIRSTLPLIASRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
BASE COUNT         1042 a          988 c         1007 g         1165 t
ORIGIN      
        1 atctctgagg taacgggttt ttgacggttc aggtatggtt cgatcggctg ccaatcaatc
       61 acccggtcca acttcaatag cgggaaacgg tcgatgtgtt tggcaattat ggcttgtgcg
      121 gtttgccgga agaaggtgct catgagaaat cccctaaatg tcttggtggg aatttagggg
      181 attttgggga tttttgcaaa ggtttccgcc tgaaacatta tgagatttca ggcggcattg
      241 gattgcttgg cggaatattt ttaaaaaggc ttacgcgccg taaacggggt atttattgca
      301 caaagcagtt acttgtttgc ggactttggc gaggttggct tcgtcttcgg ggttagacaa
      361 tacgtcggca accaagttcg ccaatacgcg tgcgtcggct tcgttaaaac cgcgtgtggt
      421 catggcagcg gagccgatgc ggatgccgga ggtaacgaag ggtttttccg gatcgttcgg
      481 aatggcgttt ttgttgacgg tgatgtgcgc tttgcccaaa gcggcttcgg cggctttgcc
      541 ggtaattttc atcggttgca ggtcgacgag gaaaacgtgg ctttcggtgc ggccggaaac
      601 gatgcgcaaa ccgcgtttga ccagttcttc cgccatggcg gcagcattga ttttcacttg
      661 ttttgcgtat tgtttgaact cgggttgcaa cgcttcttta aacgctacgg ctttggcggc
      721 gatgacgtgc atcagcggac cgccttgcag gcttgggaag atggaagagt tcagtgcttt
      781 ttcgtgggta ttgtcgcggc acaaaattac gccgccgcga ggaccgcgta gggttttgtg
      841 ggtggtggtg gtcacgaagt cgcagaatgg cacggggttg ggatattcgc cgccggcaac
      901 cagaccggca tagtgcgcca tatcgacaaa gaggtatgcg ccgactttat cggcgatttc
      961 gcggaatttt gcccagtcga tttgcaacgc gtaggcagac gcacccgcca caatcatttt
     1021 gggtttgtgt tcgagagcca agcgttccac ttcggcataa tcgagcactt cgttttcatc
     1081 caaaccataa gtaacggcat tgtagagttt gccggaaata ttaacgctcg cgccgtgggt
     1141 caggtggccg ccgtgcgcca aagacatacc caaaatggtg tcgcccggtt ttaaaacgga
     1201 agcgtacacg gcttggttgg cttgcgagcc ggagtgcggt tgaacgttgg cgtattgcgc
     1261 gccgaacagt tttttgacgc ggtcgattgc caattgttcg acaatatcga cgtattcgca
     1321 gccgccgtag tagcgtttgc cggggtagcc ttcggcgtat ttgttggtca gctgggaacc
     1381 ttgcgcttcc attacggcgc agctgacgta gttttcagag gcaatcagct cgacgtggtc
     1441 ttgctggcgt tggtcttctt gggcaatggc tgctgccaaa tcggggtcat attgtgcgag
     1501 ggtaacgctt tttgaaaaca tgttctcggc tcctttgtgt aatcagggta tcatgagtgt
     1561 tttttgtata aaaaaatact tcaaaaccta aggcagatag tccataatgc gtaaattttc
     1621 tttggcatta tcaggtaatt tatttaacat gctggttttt agcgtctcat tacctttatg
     1681 ttagtgcaag gctacccaaa ggcaatgcat caaatggtaa attttcggca gtttgtgcaa
     1741 aatcaatcaa ataaccagca ccaaccacat ctttatcagc cataacccgc tgatacacca
     1801 cttgccaaga caactctcct gccccaccat gtcttaaaat cagatgaatg gcttgtaatg
     1861 ctgtgtttgg cgtctcacaa attgcgaaga tctgctcaga agcttgcttg atggcttgta
     1921 tttttgtttg attactcaca atttccccct attttaatat taacttaatg cggtcaaatt
     1981 cacaaaatac aagcttcacc tctaatcacc catcactcga ccttctcggc gtggatcggc
     2041 accaccaacc agcctgcttg gctcgataat aatggcttga acacctgaat ttagctcacg
     2101 cacgtcagtc ttatagccca aatcatttaa tgcttgttgc cactggaggg cggttgtacc
     2161 cgtttctagt tcatagctac caaagcgatt taataaattg ggtgcactga tggcatcttg
     2221 gatatccata ttccagtcac tatgtgccac aatcgtctta gcgacatagc caatgatacg
     2281 gctaccacct ggggagccga ttgccatata aggcttgcct gctttaaata cgatggttgg
     2341 tgccattgag gagcgtggtc tcttgccggg ctcgacacga ttggcgacct gtttgccctg
     2401 ctttattggc tcaaaactaa agtctgtcag ctcattattc agcaggtagc catttgccat
     2461 caaagttgag ccaaacgcat tttcaatgga agtcgtcatt gatagcacat tgcccgcctt
     2521 atccacaatt gatatatgac tggtagaagg taactcaatc gcttgtgagg acacccactc
     2581 atgaataaaa tcgcctgcag atacgctagg caatgcctta tccgactgct caagcagctg
     2641 gctgcgatgt tttaggtagt ctttagaaat caactggcga atgggtactg gtacaaaatc
     2701 agggtcgccc aaatatacat cacgatccgc aaacgcaagc ctagaagcgt cgcccaagag
     2761 acgtaaacct tcagcatcat accccacctg attgggtgaa aattcattta aaatccccaa
     2821 aatctgaccc acagcaatcc cacctgagct tggtgcaccc ataccgcata cttcataaat
     2881 acgataagtc acacaaacag gcgggcgttc caccacttga taatcagata aatcttgtaa
     2941 ggataattga ccggggttat ccttagcatt ttggacaact gaaacgatat tttgggcata
     3001 tttaccagta tgcagagctt ttgcaccttg agctgctaac gcctgaacac tgtcagcaaa
     3061 ttctaaattt ttcagcaagc tgcctgcttg tagcggcaca ccattcggca aaaaataagc
     3121 ggctgttttt ggatagcgtg ccaaatgctg ctgattttgc tcaaccgaga tggcaagcct
     3181 tggcgacacc tcaaagcctt gttttgccaa gcggatcggt gtatcaaata attttcccca
     3241 aggcaataca ccgtatcgct gatgtattgt ctccatcagt ttagggatag caggcgtacc
     3301 caccgagcga ccaccgacca ccgcttccat aaatttcaat ggttgaccat ctttatccaa
     3361 aaataattcc ggcgtcgcac gcatcggtgc cgtctcacgc ccatcaaatg tggtcaatgt
     3421 tttggcggta ttatcccaat acaacacaaa tgcaccaccg cccaagcctg acgactgtgg
     3481 ctctaccaag cttagtgtcg tctgcaccgc caccatcgca tctgcagcgc taccgccttg
     3541 ctttaagata tcatagccag cttgtgttgc taatggattg gctgacgcta ccataaaatc
     3601 acttgcaatc acctgctttt gttcggtcag tcccgttgca tgttcaggcg tgtgagcgtc
     3661 tgcacctgtg atgacagcag aatgagtatt aaccttacct tgattggcat ggatgacttg
     3721 acatccagag attgtcatag acattatcaa tgcagtcaat aaatatgttt tagccacaag
     3781 cactccttcg cctgagtttg attgatgatt catacaaggc atgctgatta ttgtatttaa
     3841 tatggctaaa taattcaatc caaactatca atcttgacca tcaaaaaaag accgctaatg
     3901 tcatcagcag tcttttttga tatttatttt aagatattaa gtaatcagac ctttgggcta
     3961 tgctcttcaa tgagtggttt tagctcacct gattggtaca tttgtaggat aatatcacta
     4021 ccaccgatta actcaccatt aacccaaagc tgtggaaagg ttggccgact ggcgatgagt
     4081 ggtagagtac tgcgaatttc tgggttttct aggatattaa caaaagcaaa gggtctgcca
     4141 attgggtcag cacctctact gcacgcgctg aaaatccaca ttggggaaac tggggcgtgc
     4201 ct
//
LOCUS       DAWHRA010000146         4119 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWHRA010000146 DAWHRA010000000
VERSION     DAWHRA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.146"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            43..675
                     /locus_tag="WIB30_001852"
     CDS             43..675
                     /locus_tag="WIB30_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ3797865.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="WIB30_001853"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="WIB30_001853"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ3797866.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKER
                     FSGVPEVAAASEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTGMAHEL
                     AAVPMMSRTHGQPATPSTLGKEIANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQAISRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2197..4065
                     /locus_tag="WIB30_001854"
     CDS             2197..4065
                     /locus_tag="WIB30_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ3797867.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
BASE COUNT          941 a         1091 c         1069 g         1018 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcggtc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 atggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttcagag tacggcctga
      841 tgaaggcgcg cgtcaaagtc gaattaaact ggctcaaagc cctggccgcc gagccgaaga
      901 ttgtcgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgca gccgctgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg cttaaagaac gcttctccgg cgtgcctgaa gtggcagctg
     1081 ccagcgagtt catccacttt gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa actggccgaa atcatcgaaa
     1201 aactgaccgg catggcgcat gagttggctg ccgtcccgat gatgagccgc acccacggcc
     1261 agcctgctac accaagcaca ctgggtaaag aaatcgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gcgcaagagt tcctcggcaa aatcaatggc gcagtcggca
     1381 actacaatgc ccatatggtc gcttatcccg atgtggattg ggaaacccat tgccgcaact
     1441 tcgttgaaat cagcctcggc ctgaccttca acccctacac cattcaaatc gagccgcacg
     1501 actatatggc ggaattcttc caagccatca gccgtgtcaa cacgattctg atcgacttca
     1561 accgcgatgt ttggggctat atttctttgg gttacttcaa acaaaaagtc aaagcaggcg
     1621 aagtcggttc ttccaccatg ccgcacaaag tcaacccgat cgactttgaa aactccgaag
     1681 gcaacctcgg tatggcaaac gccgtattgg gcttcttatc cgaaaaactg ccgatttccc
     1741 gttggcagcg cgacctgacc gacagcaccg tattgcgcaa tatgggcgta ggcgtgggct
     1801 atgccgtgtt gggtttcgcc gcccatctgc gcggtctgaa caagctcgaa cccaaccctg
     1861 ccgcgcttgc cgccgatttg gacgctactt gggagctgct cgccgagccg attcaaaccg
     1921 taatgcgtcg ttacggcgta gccaatcctt atgagaagct gaaagacctg acgcgcggca
     1981 aagacggcat tacgcctgaa gtgttgaaac tctttatcga gtcgttggaa attccggcag
     2041 aagccaaagc caaattgctt gagctgaccc ccgcactgta tgtgggtaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacggggcg atatttatgc aagctgtccg atacaggcct
     2221 gaaattgacg gattgcgggc cgttgccgtg ctatccgtca ttattttcca cctgaataac
     2281 cgctggctgc ccggagggtt tttgggggtg gacattttct ttgtcatctc gggattcctc
     2341 attaccggca tcattctttc tgaaatacag aacggttctt tttctttccg ggatttttat
     2401 acccgcagga ttaagcggat ttaccctgcc tttattgcgg ccgtgtcgct ggcttcggtg
     2461 attgcctctc aaatcttcct ttacgaagat ttcaaccaaa tgcggaaaac cgtggagctt
     2521 tctgcggttt tcttgtccaa tatttatctg gggtttcagc aggggtattt cgatttgagt
     2581 gccgacgaga accccgtact gcatatctgg tctttggcag tagaggaaca gtattacctc
     2641 ctgtatcctc ttttgctgat attttgctgc aaaaaaacaa aatcgctacg ggtgctgcgt
     2701 aacatcagca tcatcctatt tctgattttg actgccacat cgtttttgcc aagcgggttt
     2761 tataccgata ttctcaacca acccaatact tattaccttt cgacactgag gtttcccgag
     2821 ctgttggcag gttcgctgct ggcggtttac gggcaaacgc aaaacggcag acggcaaaca
     2881 gcaaatggaa aacggcagtt gctttcatca ctctgcttcg gcgcattgct tgcctgcctg
     2941 ttcgtgattg acaaacacaa tccgtttatc ccgggaatga ccctgctcct tccctgcctg
     3001 ctgacggcac tgcttatccg gagtatgcaa tacgggacac ttccgacccg catcctgtcg
     3061 gcaagcccca tcgtatttgt cggcaaaatc tcttattccc tatacctgta ccattggatt
     3121 tttattgctt tcgcccatta cattacaggc gacaaacagc tcggactgcc tgccgtatcg
     3181 gcggttgccg cgttgacggc cggattttcc ctgttgagtt attatttgat tgaacagccg
     3241 cttagaaaac ggaagatgac cttcaaaaag gcatttttct gcctctatct cgccccgtcc
     3301 ctgatacttg tcggttacaa cctgtacgca agggggatat tgaaacagga acacctccgc
     3361 ccgttgcccg gcgcgcccct tgctgcggaa aatcattttc cggaaaccgt cctgaccctc
     3421 ggcgactcgc acgccggaca cctgcggggg tttctggatt atgtcggcag ccgggaaggg
     3481 tggaaagcca aaatcctgtc cctcgattcg gagtgtttgg tttgggtaga tgagaagctg
     3541 gcagacaacc cgttatgtcg aaaataccgg gatgaagttg aaaaagccga agccgttttc
     3601 attgcccaat tctatgattt gaggatgggc ggccagcccg tgccgagatt tgaagcgcaa
     3661 tccttcctaa tacccgggtt ccaagcccga ttcagggaaa ccgtcaaaag gatagccgcc
     3721 gtcaaacccg tctatgtttt tgcaaacaac acatcaatca gccgttcgcc cctgagggag
     3781 gaaaaattga aaagatttgc cgcaaaccaa tatctccgcc ccattcaggc tatgggcgac
     3841 atcggcaaga gcaatcaggc ggtctttgat ttggttaaag atatccccaa tgtgcattgg
     3901 gtggacgcac agaaatacct gcctaaaaac acggtcgaaa tacacggccg ctatctttac
     3961 ggcgaccaag accacctgac ctatttcggt tcttattata tggggcggga atttcacaaa
     4021 cacgaacgcc tgcttaaatc ttctcgcgac ggcgcattgc agtagcctgc cttgccgtcc
     4081 gatatcgttt gtgccgccgt ttgcctttcg gggcggcgg
//
LOCUS       DAWHRA010000147         4098 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWHRA010000147 DAWHRA010000000
VERSION     DAWHRA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.147"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(403..1215)
                     /locus_tag="WIB30_001855"
     CDS             complement(403..1215)
                     /locus_tag="WIB30_001855"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ3797868.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            1405..1866
                     /gene="tsaE"
                     /locus_tag="WIB30_001856"
     CDS             1405..1866
                     /gene="tsaE"
                     /locus_tag="WIB30_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ3797869.1"
                     /translation="MPDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLEPFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            1863..3113
                     /locus_tag="WIB30_001857"
     CDS             1863..3113
                     /locus_tag="WIB30_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ3797870.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
                     PNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMLDPGHGGEDPGAISPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKQG"
     gene            3193..4011
                     /locus_tag="WIB30_001858"
     CDS             3193..4011
                     /locus_tag="WIB30_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3797871.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
BASE COUNT          995 a         1205 c          999 g          899 t
ORIGIN      
        1 gggaatgacg gtttagaagt tacccgaaac ctcaaaaaaa ccgaaaccga acggtctgga
       61 ttcccgcctg cgcgggaatg acgggtttcg agattacagt gtatcgagaa ggatgggaaa
      121 cggtgggaat tgtgtaaaaa atgccgtctg aagcctttca gacggcattt gcggcattcg
      181 gacatttaaa aatcccatca ttcccgcgca ggagggaatc cgaatacatc tgcacagaaa
      241 cctgcatccc gtcattcccg cgaaagcggg aatctagaac gtaaaatcta aagaaaccgt
      301 tttatccgat aagtttccgc accgacaggt ctagattccc actttcgtgg gaatgacggg
      361 attttaggtt tctgtaatct cgggcagctt tccgtcatcg ttttaaccca aagacaccat
      421 ttcaatctgc tccatcgtcc tgcccagaaa acgctcgccg atggttctga atttcaaagg
      481 aatatcgctg acataaaaac ggtagtcggg attgttgttg tcggtattga gcaatccttc
      541 ctgcgcaagg acgcgtgcgg tttcttcggc agtcgtaatc gcggaatcga ccagcgcgac
      601 attgtgcgcc tccctgccga ttaagggttt gagcaaggga aagtgcgtgc agcccaacac
      661 cagcgtatcg atgccgtctg caagcaatgg tttgaggtat tcgcatacgg tcaggcgggt
      721 aacttcgtgt tccaaccaac cttcctccac caaagggacg agcagcggcg cggcctgcgt
      781 gcggacaagc gtgtcggggt tgtccctatg gatggcgcgc gcataagcat tgctgttaac
      841 tgtggtattg gtggcgataa tgccgatttt gttgttgcgc gtcgttgcca acgcggcttt
      901 cgcgccggcg gaaatcacgt ccaaaaccgg catattgccc gtcttttgac ggattttctg
      961 ccccgccacc gccgcaatcg tattacacgc gataaccatc gccttgacat cgtgttccaa
     1021 taaaaaatcg acaatctgca tcgagaaatt ttcgatggtc gccttagatt tcgtcccgta
     1081 aggcacgcgc gccgtatcgc cgaaataaat gatgttctcc atcggcagcc gctccatcag
     1141 tgcgcgcaca ttggtcaaac cgccgattcc cgagtcaaaa acgccgatgg gtcgctgcct
     1201 gccgatattt tccatccttt ttccaatccg tcctaaacat acaatccgcg tattgtacac
     1261 gcgccgcttt ttcttgacag ccgttgccgt ctgaaagcag gatgcggatt tggctgttat
     1321 aatgtacggc aatgctgccg ccgggcgcac ggtttgccga ccgcctgccc ctccgatccg
     1381 ccacataaaa gttgtaaagc ccctatgcct gattttcccc ccgtttcccg attccttgcc
     1441 gacgaagccg ccacactcga tttgggcgcg gcgtggtctt cccgtttaaa cgcaccgctg
     1501 gtcatttatc tcgaagggga tttgggtgcg ggcaaaacca cgctgacacg cggcatcctg
     1561 cgcggattgg gtcatcaggg cgcagtcaaa agcccgacct acgccatcgt cgaatcttat
     1621 ccgctggaac cctttaccct gcaccatttc gacctctacc gcttctcgtt ccccgaagaa
     1681 tgggaagacg cggggcttga cgaactgttt gccgcaaaca gcgtctgcct gatcgaatgg
     1741 ccgcaacagg gaggcgaatt taccccgccc gccgacatca ccgcaacatt gacacacgac
     1801 ggcggcggca gaaaatgcct gctgaccgcc cataccgaac gaggacgcga aagcctgccg
     1861 ctatgaccaa actgacacga agacaaatca tccgccgcac cgccggcaca ctgttcgccc
     1921 taagccccat cgcatccgcc gttgccaaaa cggtacgcgc cccgcagttt accgccgcac
     1981 ggatatggcc gtcgcacacc tacacccgcc taacgctgga aagcaccgcc gcgctcaaat
     2041 accagcactt cacgctcgac aacccgggca ggctggtcgt cgatatacaa aacgcgaaca
     2101 tcaataccgt attgcacgga ctgtctcaga aggttatggc ggacgacccc tttatccgca
     2161 gcatacgcgc gggtcagaac acaccgacca ccgtccgcct cgtcatcgat ttgaaacagc
     2221 ccacccacgc acaagtcttc gcgcttccgc ccgtcggcgg ctttaaaaac cgcctcgtcg
     2281 tcgatctcta tccgcaaggg atggatgccg acgatccgat gatggcgctg cttaacggca
     2341 gcccgaacaa aacccggcgc ggctctccgg aagccgacct cgctcaaaac actcccgcac
     2401 aaaccgaacg cggcaaaaac gggcgcagac ccgtcatcat gctcgatcca ggacacggcg
     2461 gtgaagaccc cggcgccatc agcccgggcg gtttgcagga aaaacacgtc gtcctctcca
     2521 tcgcccgcga aaccaaaaaa caattggaag ccttgggtta caacgtcttt atgacgcgca
     2581 acgaagacgt gttcatccca ttgggcgtgc gtgtcgccaa agggcgagca cggcgggcgg
     2641 acgtattcgt ctccatccac gccgatgcct tcaccagccc ctccgcgcgc ggcacggggg
     2701 tttatatgtt aaacaccaaa ggcgcaacca gctctgccgc caaattcttg gaacaaaccc
     2761 aaaataatgc cgacgcggtc ggcggcgtac cgaccagcgg caaccgcaat gtcgataccg
     2821 ccctgctcga tatgacccaa accgccacgc tgcgcgacag ccgcaaactc ggcaaactgg
     2881 tgcttgaaga attgggcagg ctcaaccact tgcacaaagg cagggtggac gaagccaatt
     2941 tcgccgtttt gcgcgcaccc gatatgccgt ctatcctggt cgaaaccgcc ttcctgtcca
     3001 accctgccga agagaagctg ctgggcagcg aatccttccg ccgacagtgc gcccaatcca
     3061 ttgcctcggg tgtccaacgc tacatcaaca cttccgtatt gaagcagggt tgattgaaaa
     3121 ggcggtgttt caatatagtt gtcggatgaa ggcaggcata aacctacttc cgccccaaac
     3181 cgcgttttta tgatgaaagg tatggataaa ttgagatacc aacaggattt tttaaatatc
     3241 cgccccattt ttacagccgg cgaacaagaa tacctgaccg aattgtccga tcggcttcct
     3301 ttatctgttt taacggactc cgtgcgtaat attgaagaaa tcggtatcga ttttgtgtac
     3361 agttctgcca aattggaagg aaacacttat aatcaatacg atacgcaggc attgcttaaa
     3421 ctcgggcaaa ccgcaggcgg gaagttatat tcggatgccg taatgttaat caacctgagg
     3481 gaaagttacc ggcacctgct gtccggtttg gacagccccg atccgtttga ctggttggat
     3541 ttcttaaaaa ccacccacag cctgatttcg gaaaacttac tggaaaaagg ctccggcggc
     3601 gtagtccgcc gcgacagcgt tactataagc gggacagact acacgccttt atccaacccg
     3661 caaagtttgg atacggaatt aaaatggttg cttcaagaag cccccaaaat agaaaatcct
     3721 ttcgaccgcg ccgtttatct ccataataat ttggcgtatc ttcaatattt taaagattgc
     3781 aataaacgta ctgccagaaa ctgcatgacc ctatcgctga tgcgctccgg tttttttcct
     3841 tgcgtattcg cccccgacag ttatcccgcc tatgccgagg ctgtcgtagc ctattacgaa
     3901 accggcgatt acggtttatt caagaaatat tttatttcgg catacgaaaa taccgtaaat
     3961 aagtacggcc cacagcctga tatggatatt tcccgcaact tctcccttta attccaaggg
     4021 gttgttatcc gtatcttccg cacgcggcaa atgcggcggc acagtggaca cacccctccc
     4081 ttaccttcgg ttatagtg
//
LOCUS       DAWHRA010000148         4079 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWHRA010000148 DAWHRA010000000
VERSION     DAWHRA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.148"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..144
                     /locus_tag="WIB30_001859"
     CDS             <1..144
                     /locus_tag="WIB30_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ3797872.1"
                     /translation="NVGIGGGALLGHWVTQYSGISCIGVAGMLTAAAGLWVCLNLNRY
                     IRT"
     gene            273..1709
                     /locus_tag="WIB30_001860"
     CDS             273..1709
                     /locus_tag="WIB30_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ3797873.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDREGTRRFWLGCGIAASLTVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHRPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWLITFHFVCLSFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1720..2703
                     /locus_tag="WIB30_001861"
     CDS             1720..2703
                     /locus_tag="WIB30_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ3797874.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPK
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAARAHHVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLATKIMEKIVFEPSTQPSSTQP"
     gene            2700..3893
                     /locus_tag="WIB30_001862"
     CDS             2700..3893
                     /locus_tag="WIB30_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ3797875.1"
                     /translation="MNPKHLIAFSALFAATQANALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRSAAADFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
BASE COUNT          871 a         1334 c         1045 g          829 t
ORIGIN      
        1 aacgtcggta tcggcggcgg cgcgctgctg gggcattggg ttacgcaata ctcgggcatt
       61 tcctgcatcg gcgttgcggg tatgctgacg gcggcggcag gtttgtgggt ctgcctgaac
      121 ctgaaccgct atatccgaac ctgaccgcac gatgccgtct gaagcccctc ccgcccttca
      181 gacggcattt tgattgaaga aatatccacc cctgcttaaa ataacgggct ttgccgtgtt
      241 ttaacgccta ttttttgttc caaggatggt ttatgccgct gctgtctgtc gagttcgcac
      301 tgttctttct cgccttcctg ccgatttact ggggcttggc gaaatacccg tccgtccaaa
      361 acctgctgct tttggctgcc ggtatgggct ggctctacca tatcggcccc gtatttgcgg
      421 caatcatcgt cctttattcc tcctgcgtgt accttttggg cgaactgctc cgttccgatc
      481 gcgaaggtac gcgccgcttt tggctggggt gcggcattgc cgcctcgctg accgtcttgg
      541 gctttttcaa atatttcgac tttttccggc cgctgattgc ccaatatgcc ggaaaaggcg
      601 gcgcaatcga catcctgatg ccgctggggc tttcgtatta caccttccag tcgctcgcct
      661 atcttgttta ctgcttccgc gccccgcacg ccgcgcgttt cgggtggcac gagctgctac
      721 tgcacctgag ttttttcccc accgttacct ccggcccgat tatccgcgcc gccgcattca
      781 aaagcgcaga cggcgagcag gcaggcgcat tggcgcaaat ccgtacccac cgaccgcgtt
      841 cgcccgtccg ccccgcactc gccgtttccc tgattctgct gggtattgcc aaaaaatggt
      901 ggctggcggg gatgctggcg gaaaactggg tgtcgcccgt atttgaaaat cccgcccaat
      961 tcgacggctg gggcgtattg gcgggcgtgt acggctatac cttccaactc tttttagact
     1021 tttccggata ttccgacttg gttatcggaa tggcaatgct gctgggcttc cggctgccca
     1081 aaaacttctc cgcaccgctt cgtgccgcga atatccgcgc attttgggac aaatggcaca
     1141 tcagcctttc cacctggata cgcgactaca tctacatccc cttgggcggc agcaaaaaag
     1201 gctttttgcg gacacagttc aacctgatgg cggcaatggt gctctcaggc atctggcacg
     1261 gctacggctg gaacttcctc atttggggcg cactacacgg cacggcgctg gtgctgctca
     1321 acacgggcga ccgctatttc ggacgcaacg cactatgccg tctgaaatac cttgcgccgc
     1381 tctcatggct cattaccttc catttcgtct gccttagctt tgtcgtcttc aataccgcca
     1441 atcccgacga tgccggcgcg gttttcagcg cgctctttgc caatgccggc ggctggaacg
     1501 cgccgcaacg ggcagacatg ctgctgcttg cctcgtttgc atccgtgatg ctgctctacc
     1561 cttacctgca acgcgctttc gacggcgcgg tcaaaggttt ggaaaaaatc ccgatgtggc
     1621 tgtggtttat ccccgtttcc gccgtcctgc tgctgattat cgtcctcgcc ccctcgggga
     1681 tacccggctt tatttatgcc aatttttaag ggtttggaca tgaaaaactt tctttccctt
     1741 ttcgcctcca tactgatgtc tgccctgatt gccgtgtggt tcagccaaaa ccccatcaac
     1801 gcctactggc agcagaccta ccaccgcaac agtccgctcg aaccgcttgc cgcctacgga
     1861 tggtggcgga gcggtgtggc gttgcaagaa aacgcctacg ccctttcaga cggcatcaaa
     1921 gccttcctgt ccggcgaaac gccgccgacg gctcaagacg gcggttcggc agatatgccg
     1981 tctgaagccg ccgcatccga agccgtccct caaaccggtg aaacagaatg gaaacaagac
     2041 accgaagccg ccgccgtccg cagcggcgac aaagtctttt tcgccggcga ctcgctgatg
     2101 cagggcgttg cacccttcgt gcaaaaaagc ctgaaacagc aatacggcat cgaatccgtc
     2161 aacctcagca aacaaagcac ggggctttcc tatcccaaat tcttcgactg gccgaaaacg
     2221 attgaagaaa ccctgcaaaa acaccccgaa atcagcgtgc tcgccgtctt cctcggcccg
     2281 aacgacccgt gggatttccc cgtcggcaaa cgatacctca aattcgcttc cgacgaatgg
     2341 gcgcaagaat acctgaaacg cgtcgaccgc atccttgaag ccgcccgcgc gcaccacgtc
     2401 caagtcgtct ggctcggcat cccctatatg aaaaaagcca agctcgacgg acagatgcgc
     2461 tacctagaca aactgctttc ggaacacctg gaaggcaaaa tcatcctgat tcccaccgcg
     2521 cacaccctga gcggcgggga agaccgctac acggactccg tcaacgtcaa cggcaaaccc
     2581 gtccgctacc gcagcaagga cggcatacac tttaccgccg aaggacaaaa actgctggcg
     2641 acaaaaataa tggaaaaaat cgtttttgaa ccaagtacgc aaccatcaag tacacagcca
     2701 tgaatcccaa acacctcatc gcattttccg ccctgttcgc cgccacgcag gcaaacgctc
     2761 tgcccgtcgc ctccgtcagc ctcgacaccg ttaccgtttc cccgtccgcc ccctacaccg
     2821 atacaaacgg gctcttgacc gactacggca acgccgccgc ctcgccttgg atgaaaaaac
     2881 tccgatccgt cgcacaaggc agcggcgaga ccttccgcat cctgcaaatc ggcgactcgc
     2941 acaccgccgg cgacttcttt accgacagcc tccgcaaacg cctgcaaaaa acatggggcg
     3001 acggcggcat aggctgggtc taccccgcca acgtcaaagg gcagcgcatg gcggcagtcc
     3061 ggcacaacgg caactggcaa agcctcacca gccgaagtgc agcagcagac ttcccgctcg
     3121 gtggcatcct cgcccacacc ggcagcggcg gcagcatgac cctgaccgca tcggacggca
     3181 agaccggcaa acagcgcatt tccctgtttg ccaaacccct gctcgccgaa caaaccctga
     3241 ccgtcaacgg caacaccgtc tccgccaacg gcggcggctg gcaggtactg gatacgggcg
     3301 cggcactgcc cctgaccata cagaccgaaa tgccgtggga catcggcttc atcaacatcg
     3361 aaaatcccgc cggcggcatt accgtttccg cgatgggcat caacggcgca caattaaccc
     3421 agtggtcgaa atggcgtgcc gaccggatga acgaccttgc ccaaaccggc gccgatttgg
     3481 ttatcctttc ctacggcacc aacgaagcct tcggcgacaa catcgacatt gccgacactg
     3541 aacaaaaatg gctggatacc gtccgccaaa tccacgacag cctgcctgcc gccggcatcc
     3601 tcatcatcgg cgcacccgaa tccctgaaaa acacgctcgg cgtatgcggc acacgtcccg
     3661 tccgcctgac cgaagtccaa cagatgcagc ggcgcgtcgc ccggcaggga caaaccatgt
     3721 tctggtcttg gcaaaacgcc atgggcggca tatgcagcat gaaaaactgg ctcaaccaag
     3781 gatgggccgc caaagacggc gtacacttct ccgccaaagg ctaccggcgc gcggcggaaa
     3841 tgctcgccga cagcctcgaa gaactcgtcc gcgccgccgc aatcaggcaa taatcggaca
     3901 ggaggcggac ggtatttccg caacaggggg atgccgtctg aaacgcatac cttcatattg
     3961 cttcagacgg cattcgcctc agacattgga aagacgcagg cgaacctccg gcatacggcg
     4021 caaaggcggc gtttgatatg ccgtctgaag gcaaagatga taaactgccg ccttccgtt
//
LOCUS       DAWHRA010000149         4071 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWHRA010000149 DAWHRA010000000
VERSION     DAWHRA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.149"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            270..1391
                     /gene="dnaJ"
                     /locus_tag="WIB30_001863"
     CDS             270..1391
                     /gene="dnaJ"
                     /locus_tag="WIB30_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ3797876.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKLGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKDPCIKCHGEGRNKTGKAVEVN
                     IPAGIDDGQRIRLSGEGEPGRHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTIPKETQTGRRMRVKGKGVKSLRSSAIGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1586..3604
                     /locus_tag="WIB30_001864"
     CDS             1586..3604
                     /locus_tag="WIB30_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ3797877.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDDRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMIIFARRVLKAGR"
     gene            complement(3648..>4071)
                     /gene="rfbC"
                     /locus_tag="WIB30_001865"
     CDS             complement(3648..>4071)
                     /gene="rfbC"
                     /locus_tag="WIB30_001865"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ3797878.1"
                     /translation="QENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREG
                     SPTFGKWTGATLSAQNQYQLWIPEGFAHGFYVLSDTTELTYKCTDYYKPEAEQVLIWN
                     DPAIGIGWPLQTAPLLSPKDLAGKTWAQAEKLRLTLSR"
BASE COUNT          867 a          887 c         1200 g         1117 t
ORIGIN      
        1 aatagggggg aagtctgcag tcgcatcaaa tgccgtctga acatgcgttc gggcggcgtt
       61 tttataacaa aaacacttca tggcggttgg ttttatgcct gtctaagttt ttgtgtcgtg
      121 catacctgaa gatttcagac ggcatcggtt tatgctgtct gaaaagtgta ttccgtttca
      181 gtttgtaagc tatggcagtc tgtttgtctt gtgttttgcg caattgccct tattttgagc
      241 cgtgatttta ttttgaatta gatgaaaaaa tgagtaatca agatttttat gcgacgctgg
      301 gtgtggcaag aacagctacc gatgatgaga ttaaaaaagc ctaccgtaaa ttggcgatga
      361 aataccatcc cgaccgcaat cctgacaata aagaggcgga agagaagttt aaagaagtac
      421 aaaaggcgta tgaaactttg tccgataaag aaaagcgtgc tatgtatgat caatatggtc
      481 atgcggcgtt tgaaggcggc ggacaggggg gcttcggagg gtttggcgga tttggtggtg
      541 ctcaaggttt tgactttggg gatattttca gccaaatgtt tggaggcggt tcggggcgtg
      601 cccagcctga ttatcagggt gaggatgttc aagtcggtat cgaaatcacg cttgaagaag
      661 cagcaaaagg cgtgaagaaa cgcatcaata ttccgactta tgaagcgtgc gatgtctgca
      721 acggcagcgg cgcgaaactc gggacatccc ctgaaacctg tccgacttgt aaaggctcgg
      781 gtacggtgca catccagcag gcgattttcc gtatgcaaca gacttgtccg acttgtcatg
      841 gtgcaggtaa gcatattaaa gacccttgta ttaaatgtca tggggaagga cggaataaaa
      901 ccggcaaggc tgtagaagtc aatattcctg caggcataga tgacggacaa cgtatccgtt
      961 tgagcgggga aggcgagccg ggaaggcatg gtgcgcctgc aggcgatttg tatgtaacgg
     1021 tgcgtatcag ggcccataag attttccaac gcgacggtct ggatttgcat tgcgaactgc
     1081 cgattagttt tgccacggct gctttgggcg gggagttaga agtgccgact ttggacggca
     1141 aggttaagct tactattcca aaggaaaccc agacaggcag aaggatgcgt gtgaaaggta
     1201 agggtgtcaa atcgttgcgc agtagtgcaa tcggtgattt gtactgccat attgttgttg
     1261 agacgcctgt taatttgacc gaccgtcaaa aagagctttt ggaagagttt gagcggattt
     1321 ctaccggctt ggaaaaccaa acgccgcgca agaaatcgtt tttagacaag ctgcgcgatt
     1381 tgtttgattg attttaaggt tcggaaacaa gcagctgtat cggggaatct ccctgatacg
     1441 gctgtttttg cttgtttgga aatagttttt attttcaatg gggtatgggc agggtgggat
     1501 aactgttttt aaccgttctt tttaaaactt gacatcatgg cgtgatgcca acaatatgtg
     1561 aacgtctgtt gtcaaaggaa gaataatgaa taaatcttta tccggttcgg tagaagaata
     1621 ccgcgaactg acgctccgag gcatgatact cggcgcattg attactgtaa tttttactgc
     1681 gtccaacgtt taccttggtt tgaaagtcgg gctgaccttt gcctcgtcga ttcccgcagc
     1741 agtgatttcg atggcggttt taaagttttt caaaggcagc aatattttgg aaaacaacat
     1801 ggtgcagacc caagcctcgg ctgcgggtac gctttcaacc atcatcttcg tcctgcccgg
     1861 tttgttgatg gcgggctact ggagcggttt cccgttctgg cagacgacgc ttttatgtat
     1921 tgccggcggg attttggggg tgattttcac cattccgttg cgttacgcga tggtggtgaa
     1981 aagcgatttg ccttatccgg aaggtgtggc ggctgctgaa attttgaaag tgggcggtca
     2041 tgaagaaggg gatgaccgtc agggcggcag cggcatcaaa gagctggcgg caggcggcgc
     2101 gttggcggga ttgatgagct tttgcgccgg aggtctgcgt gtgattgccg acagcgcgag
     2161 ttattggttt aaaagcggta cggcaatttt ccaactgccg atgggctttt cactggcctt
     2221 gttgggcgcg ggctatttgg tcggactgac gggcggcatc gctatcctgt tgggcatttc
     2281 gattgcttgg ggcattgccg tgccgtattt ctcctcacac attccgcaac cttccgatat
     2341 ggaaatggcg gcgtttgcga tgaagctgtg gaaggaaaaa gtgcgtttta tcggtgcggg
     2401 aaccataggt atcgcggcgg tttggacgct tttgatgctg ctcaagccga tggtggaagg
     2461 catgaagatg tcgttcaaga gttttggcgg cggtgcgccc gctacagaac gcgctgaaca
     2521 ggatttgtcg cctaaagcca tgattttctg ggtattgtct atgatgttta ttctaggcgt
     2581 gtcgttttac cactttatcg gcgattcgca cattacgggc ggcatggctt ggcttttggt
     2641 ggtcgtttgc acgcttttgg cttccgtcat cggctttttg gtcgccgccg cctgcggtta
     2701 tatggcaggt ttggtcggct cgtcttccag cccgatttcc ggcgtgggca tcgtgtccgt
     2761 cgtcgttatt tcactggttt tgctgctggt aggcgaatcc ggaggtttgt tggcggatga
     2821 ggctaaccgc aaatttttgc tggcactgac tttgttttgc ggctcggcag taatctgcgt
     2881 ggcttcgatt tccaatgaca acctgcaaga cttgaaaacc ggctacctgc tcaaagccac
     2941 gccttggcgg cagcaagtcg ccctgattat cggctgtatc gttggtgcgc tggttatttc
     3001 gcccgtgttg gaactgcttt acgaagccta cggctttacc ggcgcaatgc cgcgcgaagg
     3061 catggatgcg gcgcaggctt tggcagctcc tcaagcgact ttgatgacga ccatcgcgtc
     3121 gggcattttc gcccacaacc tcgaatgggt ctatatcttt accggcatcg cgattggcgc
     3181 ggtgttgatt gccgccgatt tggtgttgaa aaaatcatca ggcggcaaac ttgccctgcc
     3241 cgtccttgcg gtcggtatgg gtatttatct gccgccgtcc gtcaatatgc ccatcgtggc
     3301 aggcgcggtg ttggcggcgg tgttgaaaca catcatcggt aaaaaagcgg aaaaccgcga
     3361 aggccgtctg aaaaacgccg agcgcatcgg aaccttgttc tccgccggcc tgattgtcgg
     3421 tgaaagcctg attggtgtga ttatggcgtt tattattgcc ttctccgtga ccaacggcgg
     3481 ctcggatgcg ccgctcgcgt tgaatctgca aaactgggat gccgccgctt cttggctggg
     3541 cttggcgttc ttcgttaccg gaatgattat ttttgcacgg cgcgtactga aggcgggtag
     3601 gtagcctgtc ggagaaaatg ccgtctgaaa cgttcagacg gcatttttta tcgggaaagt
     3661 gtaaggcgga gcttttcggc ttgcgcccac gttttgccgg caaggtcttt gggcgacagc
     3721 agcggcgcgg tttgaagcgg ccagcctatg ccgattgccg ggtcgttcca tattaaaacc
     3781 tgttcggctt caggcttgta atagtccgtg catttgtagg taagttcggt cgtgtcgctc
     3841 aaaacgtaaa acccgtgcgc gaagccttcg ggtatccaaa gctggtattg gttttgtgcc
     3901 gacagggttg cgcctgtcca tttgccgaaa gtcggcgaac cttcgcgcat atcgacggcc
     3961 acgtcgaaca cttcgccgac aaccacgcgt acgagtttgc cttgtgtgtt ttcggtttgg
     4021 tagtgcaggc cgcgcaatac gcctttgccg gatttggagt ggttttcctg a
//
LOCUS       DAWHRA010000150         3994 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWHRA010000150 DAWHRA010000000
VERSION     DAWHRA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.150"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..576
                     /locus_tag="WIB30_001866"
     CDS             <1..576
                     /locus_tag="WIB30_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ3797879.1"
                     /translation="HPNITATAQTALAVAEAATTVWGGKKVELNPTKWDWVKNTDYKT
                     PAARPMQTLDGEMAGGNKPPKSITSGGKANAATYPQLVNQLTGQNLKNIAAQDPRLSL
                     ALHKSEKNFPIGTATYEEADRLGKIWVSEGARQTSGGGWLSIDGTRQYRPPTEKNSQF
                     ATTGIQANFETYTIDSNGKRNKIKNGHLNIR"
     gene            581..940
                     /locus_tag="WIB30_001867"
     CDS             581..940
                     /locus_tag="WIB30_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ3797880.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            1017..1229
                     /locus_tag="WIB30_001868"
     CDS             1017..1229
                     /locus_tag="WIB30_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797881.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            1326..2288
                     /gene="mafB"
                     /locus_tag="WIB30_001869"
                     /pseudo
     CDS             1326..2288
                     /gene="mafB"
                     /locus_tag="WIB30_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            2285..2656
                     /locus_tag="WIB30_001870"
     CDS             2285..2656
                     /locus_tag="WIB30_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ3797882.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            2762..3511
                     /gene="mafB"
                     /locus_tag="WIB30_001871"
                     /pseudo
     CDS             2762..3511
                     /gene="mafB"
                     /locus_tag="WIB30_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            3477..3683
                     /locus_tag="WIB30_001872"
     CDS             3477..3683
                     /locus_tag="WIB30_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797883.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            3704..3973
                     /locus_tag="WIB30_001873"
     CDS             3704..3973
                     /locus_tag="WIB30_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797884.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTD"
BASE COUNT         1416 a          674 c          817 g         1087 t
ORIGIN      
        1 catccgaata taactgcaac agcccaaact gcccttgccg tagcagaggc cgcaactacg
       61 gtttggggcg gtaaaaaagt agaacttaac ccgactaaat gggattgggt taaaaatacc
      121 gattataaaa cacctgctgc ccgacctatg cagactttag atggggaaat ggccggtggg
      181 aataaaccgc ctaaatctat aacgtctgga ggaaaagcca atgctgcaac ttatcctcaa
      241 ttagttaatc aattaactgg gcaaaactta aaaaacattg cggctcaaga tccaagattg
      301 agtctagctc ttcataagag tgaaaaaaat tttccaatag gaactgcaac ttatgaagag
      361 gcagataggc taggtaaaat ttgggttagt gagggtgcaa gacaaactag tggaggcgga
      421 tggttaagta tagatggcac tcgacaatat cggccaccaa cagaaaaaaa ttcacaattt
      481 gcaactacag gtattcaagc aaattttgaa acttatacta ttgattcaaa tggaaaaagg
      541 aataaaatta aaaatggaca tttaaatatt aggtaattat atgagattaa aattaaaaaa
      601 ttatgattgt tctgactttg atttggaaga tttcccacaa gacaagttag aaaatttttg
      661 tatacttttg acattatcaa taggttttga tgaaagtaat ggtgctgatt atttttatgt
      721 ttatatatat agcactgaat ggttactttc aaatatacat agaccgatgt cattaaaaaa
      781 ctcaattgtt acaaaccgtt ttaatattga acacatatta aaattgataa atgatatatt
      841 ggaaatatgt aattcaacgt ctgaagataa gtctatatca aatttggcaa aatattttga
      901 ctgggaattt gacgattata atctaaatat ccaagactaa agatggtggg acagcattta
      961 gaccaaaaac aggcaaacgg tattttgatg acgaattaaa ataaaaggaa ttattcatga
     1021 aattaattaa ttttgaagag agttcagtta aaattagtct cgataaagat gagttgtata
     1081 ttattcaagc aattgtagga gaaatttatt caggcgtatg cgtggattgt agagattttg
     1141 agataattca tggtgttgaa aaaataaagt tctttctttg gataaagaac ttaaaaaaat
     1201 atatgatact tgggataaat gttagctagg taaggtgggc acttgttgcc caccttttgc
     1261 cgtatattaa aaatcctttt caggctgcct gaaactgggt gggcatcaat gccaatccta
     1321 actgtgctga tgaagcagga aaactgatat gggaaaacga ccccaataaa aattggtggg
     1381 ctaaccgtat ggatgatatt cgcggcatcg tccaaggtgc ggttaatcct tttttaatgg
     1441 gttttcaagg agtagggatt ggggcaatta cagacagtgc ggtaagccct gtcacagata
     1501 cagccgctca gcagactcta caaggtatta atgatttagg aaatttaagt ccggaagcac
     1561 aacttgctgc cgcgagccta ttacaggaca gtgcttttgc ggtaaaagac ggcatcaatt
     1621 ccgccagaca atgggctgat gcccatccga atataacagc aacagcccaa actgcccctg
     1681 ccgtagcaga ggccgcaggt acggtttggc gcggtaaaaa agtagaactt aacccgacta
     1741 aatgggattg ggttaaaaat accggctatg aaacaactgt tacccgaaag actttagatg
     1801 ggcaaatagc tggcaagaac aagcctatta aaccttcttc aggagattat gtcttgccgg
     1861 taggcagaca aatcatagat cctactcgta catctttttc acaagctaca gttagctatc
     1921 agaagcgtgg tgcaaattac aactatgatt ctcttgtagc tgcaatgaac gaaaaaaaat
     1981 catgggttgg cgacagggtt gatgttgtta atatgcctga tggagcacct actagtatgg
     2041 ataacacgcg tattatggca gcacgtgaag caggagtaaa agtggaagcg aatgttcata
     2101 attttaatga ccgattatca tcaaaagaga gaatcaggtt taagcatgat ggtattgagc
     2161 ctcaaacttg gggagaagct atccagctac gaattagaaa gcaagaaaca caaaaaggag
     2221 ttccagaagg gtggagcaaa agatttccta acggaagtat ttatgatgta aaggtactta
     2281 ggaaatgata aaacaaaata gttttgttcc gtatcctgaa gcaatgcttc ctaaaggatt
     2341 taaatatccg caaagttatt taaaattagc tcaatccact catgccatta actacgatga
     2401 acaatattct tttccttggt ggtttgaaaa tgcagaaagc aatatatcag aagtaattga
     2461 catttatttt gaaataactg gcattccaaa cctattacct tttgctagaa accaagagtg
     2521 ggctgcctgt tttgatattt cagataaatc aggtaatcct aaaattatag tagttaattt
     2581 agataataca aaatattacg agacttttga aaattttgat acttggctaa aagaagctga
     2641 aaatgatggt tggtagcagc cgtaggtcgg attctcgaat ccgacatttt ccaacagcgg
     2701 catttcggaa acgatagacg cgtcaaatat ttttgtcgga tacaaatatc cgacctacat
     2761 ctctgcgcag caaactttac aagatattaa tgaattagga aatttaagtc cggaagcaca
     2821 acttgctgcc gcgagcctat tacaggacag tgcttttgcg gtaaaagacg gcattaattc
     2881 cgccagacaa tgggctgatg cccatccgaa tataactgca acagcccaaa ctgccctttc
     2941 cgtagcagaa gccgcaacta cggtttgggg cggtaaaaaa gtaaacctta acccgaccaa
     3001 atgggattgg gttaaaaata ccggctataa aacacctgct gcccgcccta tgcagacttt
     3061 agatggggag atggcaggtg ggaataagcc accaaaacca agtacgcagc aacaccctac
     3121 acactctgat aacaatatcg gcttacctgc ctcatatgtt aaacctgata catctatttc
     3181 tccgacagga acaattcaag accgcatcag atggacaaag tccaagtttc ctactgagaa
     3241 atctttaaat ggacatttca aagctcatgg aaaagaattt ggcgatataa ccattgaaga
     3301 ctaccaaaaa atggcgtctg atttgttatc aaaacagaca tcggacaaga tattaggtta
     3361 tcagacggaa catagacgag tgcgctatga tatcaataac aatatctatg ttttggccaa
     3421 tccaaaaaca ttcaaaatca aaacaatgtt taaaccaaac ttaggaaaga agtattatga
     3481 tggagaattc aaaaaagaca tgggaaattg acggagaaat atggctacat tgtcctgttt
     3541 gcggaactga agttatggac tatgatatct gtgacgtttg tcagtggcaa aatacaggag
     3601 aaactaatat agatggtggc cctaatgaaa tgacacttgc ggaggcgaaa gaagcttacg
     3661 caaaaggctt accaatcaga taaataagca cctagagaaa tcaatgatga cggaatccca
     3721 tggttaccta tcaaataatt tacctgttaa aatcataaat gatattattt atgcaaccca
     3781 gttagtcgaa gatttggttt taggaaaaat aaaaattgtt gattttttaa aatcatataa
     3841 caatttttat tgttggcttg gttttgatga gttgcctcaa tctgagaaaa taaaattcct
     3901 aagctatctt aatatattaa gtattcataa agaaatacaa gatgaaactg tgaatagggt
     3961 ttataccgat tgaaaaatag tagatagaga ttaa
//
LOCUS       DAWHRA010000151         3988 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWHRA010000151 DAWHRA010000000
VERSION     DAWHRA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.151"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="WIB30_001874"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="WIB30_001874"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ3797885.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="WIB30_001875"
     CDS             1160..2290
                     /locus_tag="WIB30_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ3797886.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANVTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2361..3212
                     /locus_tag="WIB30_001876"
     CDS             2361..3212
                     /locus_tag="WIB30_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ3797887.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDLEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="WIB30_001877"
     CDS             complement(3295..3957)
                     /locus_tag="WIB30_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ3797888.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          867 a         1169 c          983 g          969 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccgg acaaacgggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaccctgcc
      181 gtcgatgatg tgcgtggctt tcacgccgtt gacggcggct tcgaccgcag aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 gagtttttcg gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccacgttc
      481 tatcagccct ttaaccaaac ctgtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 tacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatggtcgtc
      601 gcgtccgctc acgccgaccg cgtgcccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacgtgc ccgcccaaca ccatttcgac aatatccatc gcctctttgt cggtaacgcg
      721 cattccttgg acaaactcgc cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgcca tgaacgatga cgggatgaat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtatt tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgctttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacgactccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatccggca gaccttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgacc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaat catacgccgc tgtccaacag cgtgtattac gcctatttcg aaccttactc
     1501 cgaagaacag catttaaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aagggcgcga catcaatctg ctgaccatcg gcaatcaggt
     1621 cgaaagcgat ctgaaaatct ggattaccgc ccgccagcat ccgggcgaaa ccatggcgga
     1681 atggtttgtc gaggggctgc tcggcaggct gctcgatccg caagacccga cagcgcgcac
     1741 cctgctcgac cgcgccacgt tctatatcgt ccccaatatg aaccccgacg gttcggcact
     1801 gggcaacctt cgtaccaacg ccgccggtgc gaacctcaac cgcgaatggg aaaatccgac
     1861 tttggaaaaa agccccgaag tgttcttcgt gcgtgaaaga atgctggaaa ccggcgtgga
     1921 tttgtttttg gatatccacg gcgacgaagg gttgcccttt gtctttgttg caggaactga
     1981 aggcgtgccg aactacaatc cgcgcatttc cgcgttggaa acacagttca aaaccgccct
     2041 tttgaacgcc agccccgatt tccaagatga atacggttac gaaaaagatg cgcccggtca
     2101 ggcaaacgtg accttggcaa caaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gttggaaatg ccgttcaaag acaatgccaa cctgcccgac gacgacttcg ggtggaacgg
     2221 tcaacgttcg ttgcgcctgg gcgaagccgc gttgtccgcc atcctcaacg tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcctgtt atgattatcc atccccaatt cgatcccgtc ctcatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgcttg
     2521 acgacttcct gacatggggc attttgggcg taatttttgg tgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatatgg ttgttcggcc
     2701 gcaaacacgg catcggcttc ctcaaactga tggacacggt cgcaccgctc gttccgctgg
     2761 gtctcgcttc gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagac ctcgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcgccgtc gtttggctgt
     3001 tctctaaaaa acagcggccg accggacaag tcgcctcgct cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa tttgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gcatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 tgttttttcg ccttccctgc ggacggtttg cggcttgttg atgaccgcct gccagtagcg
     3421 gtagatatcc gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt
     3481 gaattgcaga cggatagtgc cgttgtcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa acgcttcatc atcgacggct tcaaaccaat cggtcggcac
     3601 aatatccaaa ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aatttttggg aatccatcat
     3961 tttccttttc aaatgggttt tgcgccct
//
LOCUS       DAWHRA010000152         3969 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWHRA010000152 DAWHRA010000000
VERSION     DAWHRA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.152"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            150..1667
                     /locus_tag="WIB30_001878"
     CDS             150..1667
                     /locus_tag="WIB30_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ3797889.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSLAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1726..3507)
                     /locus_tag="WIB30_001879"
     CDS             complement(1726..3507)
                     /locus_tag="WIB30_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ3797890.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHAVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDDLPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQIGKVCVPEPFKVSRFGSVWQMTSTIQAQALPDTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEPRGLYTGSIGYLNPSSGGLGFEGAFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENRQCALLDRHLCRLKTSAQALNLPLPDGCENQIKQYIAHLPDGVFRVKARLASDG
                     ISLSRAVLNRLTDKQRVIISPAVLPAQNYLRRFKTTHRALFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          822 a          889 c         1306 g          952 t
ORIGIN      
        1 atgtgaaatc cgccctttga aaatcgggcg gcggtttttt gcctgcccgc tttcagacgg
       61 taaagccggt tttcacgggt ttctgcctgt ttttcggatg gtttgacgtg cttcggcggc
      121 gtgtttgcca gaaaggtaaa tgacagggta tgttgtattt cagatacggt tttttggttg
      181 tttggtgtgc ggcaggtgtt tctgccgcct atggggcgga tgcgcccgcg attttggatg
      241 acaaggcatt gttgcaggtg cagcggtcgg tgtcggataa gtgggcggaa tcggattgga
      301 aagttgaaaa tgatgccccg cgcgtggttg acggggattt tttgttggcg cacccgaaaa
      361 tgttggaaca tagtttgcgc gacgcgctca acggcaatca ggcggatttg atcgcttcgt
      421 tggcggattt gtatgccaag ctgccggatt atgacgcggt tttgtacggc agggcgcggg
      481 ctttgctggc gaaattagcg ggaaggccgg cggaggcggt ggcgcggtat cgggaactgc
      541 acggggaaaa tgcggcagac gagcggattt tgctggattt ggcggcggcg gagtttgacg
      601 atttccgact gaagtcggca gaaaggcatt ttgcggaggc ggcaaaattg gatttgccgg
      661 cgccggtttt ggaaaatgtg gggcgttttc ggaaaaaaac ggaggggctg acgggctggc
      721 gtttttcggg cggcatcagc ccggcggtca atagaaatgc caataatgcc gcgccgcagt
      781 attgccggca aaacggaggc cggcagatat gcagtgtcag ccgggcggag cgggcggcag
      841 ggttgaatta tgaaatcgag gcggaaaaac tgacggcgtt ggcagataat cattatttgt
      901 tgttccgttc caatatcggc ggcacgagct attatttcag taaaaaatca gcttatgatg
      961 acgggttcgg cagggcgtat ttgggttggc agtataaaaa tgcgcggcag acggcgggga
     1021 ttttgccgtt ttatcaggtg cagttgtcgg gcagcgacgg ctttgatgcg aaaacaaaac
     1081 gggtaaacaa ccgccgcctg ccgccgtata tgctggcgca cggagtcggc gtgcagctgt
     1141 cccatactta ccgcccaaac ccgggatggc aattttcggt cgcgctggaa cattaccgcc
     1201 aacgctaccg cgaacaggat agggcggaat acaataacgg ccggcaggac gggttttatg
     1261 tttcgttggc aaaacgtttg ggtgaatcgg caactgtgtt cggcggctgg cagtttgtgc
     1321 ggtttgtgcc gaaacgtgaa acggtgggcg gcgcggtcaa taatgccgcc taccggcgca
     1381 acggtgttta tgccggttgg gcgcaggagt ggcggcagtt gggcggtttg aacagtcggg
     1441 tttctgcgtc ttatgcccgc cgcaactata agggcattgc ggctttctcg acagaggcgc
     1501 aacgcaaccg cgaatggaat gtctcgctgg ctttgagcca cgacaagttg tcgtacaaag
     1561 gtatcgtgcc ggcgttgaat tatcgtttcg gcaggacgga aagtaatgtg ccgtatgcga
     1621 aacgccgcaa cagcgaggtg tttgtgtcgg cggattggcg gttttgaatg gtgggataat
     1681 gccgtccgaa ctttggaaac aggttcggac ggcatttttg cgcgttcagg caagggcggc
     1741 ggcaaatacg ccgcgcaggg cgttggagag gcggatttct tcggcttctt gcagtgtttt
     1801 ttgtgtgatg tgtgtttcga ttacttgatt tgtttgcaaa tatttttgcg gttcgtccaa
     1861 tacggcttgg cgcattatgc cgtttaaaat gtctaaatct aaagagggcg taagccattg
     1921 tccgcgatgt ttgacgaaca cgttgcttct gccgccttcg agcaggatgc cgtccgaatt
     1981 gaaaaacagg ctgtcgaacg cgccttgtgt ttcggcggtt tgccacgctt ggtcgaagag
     2041 ggcgcggtgt gtggttttga agcggcgcag gtagttttgt gcgggcagga cggcgggcga
     2101 aatgatgacg cgttgtttgt cggtcagacg gtttaaaacg gcgcgggaca ggctgatgcc
     2161 gtctgaagcg agccgggctt tgacgcggaa cacgccgtcg ggcaaatggg cgatgtattg
     2221 tttgatttga ttttcgcaac cgtcgggcag gggcaggttg agggcttggg cggaggtttt
     2281 cagacggcat aggtggcggt cgagcagggc gcattgcctg ttttccacgc gcagggtttc
     2341 aaaaatgccg aagtcggggc gcaattcgtt gaggaaacgg gctttccagc cgcattcccg
     2401 atattcggcg gcagggtcgc tgtcgatgac gatgccggaa ccgacaccgt acacgccttg
     2461 ataaatgccg tctgaaagcg gcgtgagcga taaggtgcgg atgacgacgt tgaacgcgcc
     2521 ttcaaacccc aagccgccgg aactcgggtt caaatagccg atgctgcccg tataaagtcc
     2581 gcgcggttcg gcttcgagag attcgataat ctgcatactc atttttttgg gcgcgccggt
     2641 gatgctgccg caggggaagg cggcgcggag gatgtcggcg aacgaggtgt ccggcagggc
     2701 ttgggcttgg atggtgctgg tcatctgcca aacgctgccg aaacgtgata ctttaaacgg
     2761 ctcgggtacg catactttgc cgatttgggc gattttgccg aggtcgttgc gcagcaaatc
     2821 gacaatcatc acgttttcgg cgcggttttt cgggtcggct tgcaattcgg cggcgcggcg
     2881 ttcgtcttgt ccgtcgccca aaatcggcgc ggtgcctttc atcggttcgg tgctgatggt
     2941 gccgtctgaa gcgatgttga ggaagagttc gggcgagaaa cacagcgtcc acgcggattg
     3001 cccctccgca tcgggcaggt gggacaagac ggcatagggg acgggctggc gcaggcggcg
     3061 gtagaggctg acgggattgc cgtaggcttg caggtgcagg cgagtggtgt agttgatttg
     3121 ataggtgtcg ccgcgccgga tggcttcgtg gatttggcgg atgcggtcga ggtaatcggt
     3181 ttcggatacg gaggattgcg gcgtggaaat gccggcggga aggtcgtctg agtgtcgggc
     3241 aagccagctt gcggcatcgg tgtcggcgca gtcggcaaac cagtgcaggg caaggttgcc
     3301 gccgcgctcg gactcaaccc ccgtcagcgg caaaccgaat ccgtagtctg caaacaacac
     3361 ggcatgcagc cctttttgcc agcccgattg cagcgcgccg tccaaagcat cgagttcttc
     3421 gggacggaaa aaacggcttt ccacatgatt ttgatagcgt tttgcgcggc cgcttacggc
     3481 atcgtcaaac agggcgaaat aaggcatggc aattcggggc aaatgttttg attatacgct
     3541 ctttttacac atgttttcag acgattcggc aaatatcggc aaaatgtaat tttatgtaga
     3601 gaaagtgggg ggcgaaggtg taaaattgtg aaaacaaatt ccgatttcca acctgaaata
     3661 caataaggag acctttatga cagaccacca gctgcaaccg tttgaaaacg tagaattagg
     3721 cgaaaagcaa gaccagctcc aagtatagtg gattaacaaa aaccagtaca gcgttgcctc
     3781 gctttagctc aaagagaacg attctctcag gtgctgaagc accaagtgaa tcggttccgt
     3841 actatctgta ctgtcttcgg cttcgttgtc ttgtcctgat ttttgttaat ccgctataaa
     3901 gaccgtcggg catctgcagc cgtcattccc gcgcaggcgg gaatctagtc tgttcggttt
     3961 cagttattt
//
LOCUS       DAWHRA010000153         3962 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWHRA010000153 DAWHRA010000000
VERSION     DAWHRA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.153"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(406..651)
                     /locus_tag="WIB30_001880"
     CDS             complement(406..651)
                     /locus_tag="WIB30_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797891.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            763..1191
                     /locus_tag="WIB30_001881"
     CDS             763..1191
                     /locus_tag="WIB30_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ3797892.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1203..1679
                     /locus_tag="WIB30_001882"
     CDS             1203..1679
                     /locus_tag="WIB30_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ3797893.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(2029..2517)
                     /locus_tag="WIB30_001883"
     CDS             complement(2029..2517)
                     /locus_tag="WIB30_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ3797894.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2585..3640)
                     /gene="aroG"
                     /locus_tag="WIB30_001884"
     CDS             complement(2585..3640)
                     /gene="aroG"
                     /locus_tag="WIB30_001884"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ3797895.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAEHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAS"
BASE COUNT          917 a         1021 c          934 g         1090 t
ORIGIN      
        1 acgggatttc aggttgctgt ttttgatttt ctgttttgtg ggaataacgg gattttggat
       61 gatgtgcatt ttcgggaaaa ctaaaaaccc tccgccgtca ttcccgcgaa agcgggaatc
      121 tagaatttta atgcggcaaa aatttatcgg aaaaaaccga agtttaaaga cctagattcc
      181 cgcctgcgcg ggaatgacgg tttagaaatc acccgaaacg agaaaaagaa ccgaaaccga
      241 acaagccgga ttaccgcctg cgcgggaatg acggctcaaa agttacgaaa cgaaaaataa
      301 ccgaaaccaa acaagtcgga ttcccgcgcg ggcgggaatg acgataagcg taatttcaat
      361 ctgccatatt gtcttttcac ttgcctgaaa acagatttca gccacctatc cctgcatccg
      421 gcggctgatg tagtcggcaa tcaaatcccg cttttcccaa aaaaacttct gcattttttg
      481 ttcggaatac agtttgcgga ccagagtggg caaaccgctg tggacgatgg cggcgatttc
      541 tttgttgcct ttttcttcct gcattgccgt ctgaaaatcc atacccctgc ctttcagata
      601 ttctgcaagg cggttgcgga tgtgggaatt gagttcgatg gatttgagca ttttgcctcc
      661 gttttgcggt aaggatttat tcgatttgtt tatccgcctg attttatcac gtttcccaag
      721 cggtttgcct gtacaatacc gcttttcggc agggtgtggg cgatgatgtt ttcttggttc
      781 aagctgtttc acttgttttt tgtcatttcg tggtttgcag ggctgtttta cctgccgagg
      841 attttcgtca atatggcgat gattgatgtg ccgcgcggca atcccgagta tgtgcgtctg
      901 tcgggcatgg cggtgcggct gtaccgtttt atgtcgccgt tgggcttcgg cgcggtcgtg
      961 ttcggcgcgg cgataccgtt tgccgccggc tggtggggca gcggctgggt acacgtcaaa
     1021 ctgtgtttgg gcttgatgct cttggcttac cagttgtatt gcggcgtgct gctgcgccgt
     1081 tttcaggatt acagcaatgc tttttcacac cgctggtacc gcgtgttcaa cgaaatcccc
     1141 gtgctgctga tggttgccgc gctgtatctg gtcgtgttca aaccgttttg acggagcatc
     1201 ttatgccgat agaaatcgaa cgccgttttt tgattggaaa cgacaactgg cggcaatacg
     1261 ccgacgaacc gctgctgttg aggcagggtt atctgtctgt tgaaaaagaa cgcaccgtcc
     1321 gtgtccgcat tgccggaaaa caggcgtggc tgacgctgaa aggctatatt tcggaaatca
     1381 gccgcagtga gtttgaatac gaaatcccgc ttgccgatgc ggaaaagatg atggaaacga
     1441 tgtgcccgtt taaaatggaa aaatggcgtt atccggtcag atggggcggc agcctgtttg
     1501 aaatcgatgt ttttcttggc gataatgcgc ctttggtcgt cgcggaaatc gagttgcccg
     1561 acgagaatgc cgattttgac cgtccggact ggctgggacg ggaaatcact gccgacggta
     1621 tgtttaccaa tgcttatttg agcaggcatc cgttctcaag ctggaaaaat gccgtctgaa
     1681 acggcttcag acggcattct ccgacaacca tcgcaaatcc aaagacagcc tctatatcct
     1741 tatttctgct ttctttgttc cattattccc aataaatccc cataacctaa aatctcgtca
     1801 ttcccgcgca ggcgggaatc cggttcttta aacttcggtt ttttccgata aattgcttta
     1861 gcttttcgtt tccggattcc cgcttttgcg ggaatgacga tggaagattg ttgttatttc
     1921 ggataaatta ccctaacttt aaaatcccgg attcccatct tcgggggaat aacaatattt
     1981 tctatttcag cccatcattt gcgggggatt ttatagagag tttatatttc aatatctcaa
     2041 ataatgcaca aaagcatagc gcgtgccttt gctgctgaca cggcgttccg tccgctctgc
     2101 ttctttccaa tgcgtccggt ctattgcggg gaaaaatgca tctccttcca cagacaaatc
     2161 cacttcggtt atccgcaaat cggtcgccaa tggcatcgct tgtccgtata tctgcgcgcc
     2221 gcccataatg acggcttctt ccgcgcctgc gcacaatgcc aatgccgcct ccaaacttgc
     2281 cgccgtttcc gcgcctgccg cgcaataatc cgcctgccgg ctgatgacga tgttcctccg
     2341 tccgggcagg ggtttgacgg gcagggattc ccacgttttc cgccccataa tgacgggttt
     2401 gcccaaggta taggctttga aaaatgcgaa atcttcgggg atgtgccaag gcatagcatt
     2461 gcccgccccg atgcacaggt tttccgcaca cgccgcaatt agggttattt tgagcatatt
     2521 tcctccacaa aaatgccgtc tgaacgcttc agacgacatt ttatgacatt ctgcgtcaaa
     2581 aatctcaact ggcgcgcgcc atacgttttt tgtttgcacc tgccaacaat gccaacagtt
     2641 cttcagtcgc gccccaaccg atacacgcat cggtaatgct cttgccgtac acttccggct
     2701 tgtcctgtct gccttcgacc aaatggcttt ccaccatcac gcccatgata ttgccgccgt
     2761 cctgttccaa ttgggcggca atgtcttgtg ccacttccat ctgccgagtg taatccttgc
     2821 ggctgttggc gtggctgcaa tctatcatca gcttgtcggt tacccctgcc gcacgcagtt
     2881 gttccgccgc ctcgctgacg tgttccgcat cataattcgg ctctttgccg ccgcgcaaaa
     2941 tgacatgaca gtcgggattg ccgccggtat ggacaatggc ggaatgcccg gccttggtta
     3001 cagacaggaa atgatgcgaa tggctcgccg caccgattgc gtcgatggca atcttcaaat
     3061 tgccgtccgt accgttttta aagccgacgg ggcaggacag cccgcttgcc aattcgcggt
     3121 gaacttggct ttcggtcgtc cgcgcaccga ttgcccccca agaaatcaag tccgcataat
     3181 attgcggcgt aatcatatcc aaaaactcgg tagaggcagg catacccata ttgttcagcg
     3241 acaacaacag gctgcgcgcc tgacgcaaac cgaaattgat gtcaaacgta ccgtccaaat
     3301 gcgggtcgtt aatcaaacct ttccaaccca ccgtcgtcct cggcttctcg aaataaacgc
     3361 gcatcacaat caaaagctcg ttttcatact gcttgcggag tttcaacaaa cgctccgcat
     3421 attccaacgc cgctttcgga tcgtgaatcg aacacggccc gataataacc aacagccgct
     3481 tgtccctgcc gtgaaccaaa tcggaaattt cctgacgggt gcggtgaacc aagcccgaag
     3541 cctctttgga aatcggcagc tcgtaaagat gggctatcgg cggcaacaac tctttaactt
     3601 ctttaatctt aatatcgtct gtggggtaat ggtgtgtcat cttgcatctt tcctgatggg
     3661 tttttatacg cggcttaggt tacatcgttt accttgcaca aacaagtgca acatattact
     3721 acaaagctat tttatttcaa aataactgat atatcttttt ttatagattt taaatttcat
     3781 atttttatta ttttagaaat tttatttcaa ataaaaagaa acagattgat aaaaacaata
     3841 cccgccattc atttggacaa acctgcacgg aacatccgtt ttcccatttc cacgacaatc
     3901 cggtacagaa cacccccgct gccgattccg ccttcacatc cggcaaaatt tcatacacaa
     3961 gg
//
LOCUS       DAWHRA010000154         3954 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWHRA010000154 DAWHRA010000000
VERSION     DAWHRA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.154"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            142..1285
                     /locus_tag="WIB30_001885"
                     /pseudo
     CDS             142..1285
                     /locus_tag="WIB30_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(1395..1604)
                     /locus_tag="WIB30_001886"
     CDS             complement(1395..1604)
                     /locus_tag="WIB30_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797896.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(1601..1897)
                     /locus_tag="WIB30_001887"
     CDS             complement(1601..1897)
                     /locus_tag="WIB30_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ3797897.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRRARL
                     AQKPKTRWKILYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(1876..2481)
                     /locus_tag="WIB30_001888"
     CDS             complement(1876..2481)
                     /locus_tag="WIB30_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797898.1"
                     /translation="MTIYFKNGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSDYHEWDGKKWKISKAAAAARFAKQKTALAFRLAEKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            2561..2848
                     /locus_tag="WIB30_001889"
     CDS             2561..2848
                     /locus_tag="WIB30_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ3797899.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            2820..3122
                     /locus_tag="WIB30_001890"
     CDS             2820..3122
                     /locus_tag="WIB30_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ3797900.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYIAQLK"
BASE COUNT          817 a         1059 c         1072 g         1006 t
ORIGIN      
        1 gcaacgctgt actggtttaa atttaatcca ctataaatta acacgcaatg taccctgacc
       61 ggcaaaactt ccccaaactt catcaaaaaa tcaactgtac ccgtcttttt cagacggcat
      121 tgataccgta agaaatttcc catgcagaaa tggcaaaccg agctttattc cacaccgctt
      181 tggctttgtc tgacaccgaa aagcgcggtc aaagtcttgc tgctgattac ggcgatgatt
      241 acgctggtgt taaccgaaat gcggctgaat gtgttgagta cctttatgtc caaagggctt
      301 tacgactcga tgcaggattt gaaatgcttc ggcattttgg atgtttgctg caatgaacgc
      361 gggcgtggtg ctgatacggg cgtttaacaa cgtcgtcaac gacttcctcg atcaaggctt
      421 ggcgattaaa tggtcggagc ggctcaacga agtgctgaca gcacgctggc ttgccgacaa
      481 aaactactac cgcctgcaaa tgcgccgcca cgcgccggac aacatcaacc agcgtatcca
      541 acaggacgcg caggaattta tcgcttcgac catcgaattt gtgcgcggca tggtcaattc
      601 ggtcgttact tctctggaat ttgccgttgt tttgcggggc ttggctggca tcctgaccgt
      661 gttcggcttc gacattccgc acggcatcgt ttggtttgtc tatatatttg tgattttggc
      721 gacctttatc gccatgtgga taggcaaccc tttgattcgt tacaattatg aaaacgaaaa
      781 actcaacggc gactaccgtt attccctcat ccgcgtgcgc gaccacgccg aaagcgtggc
      841 gttttacagc ggcgaaaaac acgaacacga ccagctttct gaccgtttta aagccattat
      901 ccgcaaccgc tggcgtattg cgcgacaaag cgtctgcctg agcggcttta atgatatgtt
      961 caccaacggc atcaagctct tccccattat tttgcaagcg ccgcgcctgt ttgccggaca
     1021 aatcaaaatc ggcgacatcc agcagaccgt ccaagccttc gcgcgactgc aaaacgcgct
     1081 ttctttcttc cggatgttct acaacaaatt caccgcctac cgcgcccgat tggagcgtct
     1141 gtacggcttt ttgctgagta ccgaagaaca acacagcgcg cggcaacccg agattagcga
     1201 agtttcagac ggcattgcac tggaatgtaa ggcggcggca gcttttgcac gaattggcgg
     1261 cagcttttgc accgccgaag cgtaggaaat tgtattagct tttgcacaaa tttatccccg
     1321 tttaatggtc tgttaaacgg ggattatctt atgcctttaa aagtagtacg ctacttgcgg
     1381 tcagaactta taaatcaggt ggtcgtcgta gcttgaattg ctgttgatgt gctgctcaca
     1441 gtcaaggttt ggctgcctgc aaaacgctcc catccggtca ggcgcagttc tgccacgagg
     1501 tcaagataag caggcagttc gctgcgggtg ccggagaaga agacaaatgg cggccgcacc
     1561 attgccatca ggcgcagaaa ctctaccatg ccgaagtagt tcatcgcgtc cccttttccc
     1621 cggcggcgcg gctttggttg aaccgcgcgt cgtgctgcct gcctttcagt tcgctgatat
     1681 acgcctgccg gcagtggtgg cggtcgaaaa acacgccgtt gatcagggta tataaaatct
     1741 tccagcgggt tttgggcttt tgggcgagcc gcgcgcggcg ataggtgcgg cttgagaggg
     1801 tttcgtccgc cgcgccgccg gttagggcgt tgaacagttg gtcgatggct accaacaggt
     1861 ggtaaacgta ttttttcagc cgatgtttag cgtccattct tcgatttcct tttccagcgc
     1921 gtccaatccg ggcgcggttt cgatggtgtt caatttgtct tcgagctgct gacgctttcc
     1981 gataatcgcg ccggcggcaa cagccaggcg ggcggatttt tcgataactt tttcaatcaa
     2041 aacgtccaat tccacgcccc ttgcggcggc gatttgcgcc agcatcgggg tcggggcgtt
     2101 gttgtccgcc tgccgcgcga gggcttcttt ttcctgcctg taaaagctgt cgatttccac
     2161 ttggggatag cccgccaaga ggctgttttt gagttcgtcc gccttttccg cgaggcggaa
     2221 tgccaaggcg gttttttgtt tggcgaaacg ggcggcggcg gcggctttgc tgattttcca
     2281 ttttttgccg tcccattcgt ggtaatcgga cgggcgcggc ggggttaaaa cggggcggcc
     2341 gtcggaatct gcggcaatct gcccgccctg cgcctgtcct gccaaaaggg cggcgtattc
     2401 ttcggcgcgg acggcaaccg cgccttcggg gatgccgccc aatgtgtcgt cgtaaaagcc
     2461 gtttttgaaa tagatggtca ttttggggtt tccttttttg tatttacatt ttggtttttt
     2521 gccgcttata atgtaacgac aaaatacgct gcaagctgca atgaagattg aatttgactc
     2581 cgaaaaaaat caacgcaata tcgaagagcg gaatttgccc tttgaatcag tcgggcaaat
     2641 acgctggaca acggccgtta ttgtgcccga tgtccgcttt gattaccccg aaccgcgtta
     2701 tgttgccgct gcttatctag gcgatacgca acgcctgcac attgtctgtt ttacgccgat
     2761 aaaagacggc atccgtgtca tcagttttcg caaagcgaat aaaagagagg ttaaaaaata
     2821 tgcaacgtca atccttaaca aacgctgacg gcgaagtgcg ggaacttgcc gccgaagatt
     2881 ttgcgcttgc ccgccccatt gccgaagccc tgcccgaaga cttggcacag gtgctttttt
     2941 cccatcaaaa acaattggaa gaaaagggcg taatgcaaaa acgaaacaca ggcaaaaccc
     3001 cgaaacaact cgttaccata cgcctgtccg ccgatgtggt cgaaaaattc cgcgccggcg
     3061 gcaagggctg gcagacgcgc atcaacgagg ttttgcgcca atatatcgcc caattgaaat
     3121 aacgggggcg gccgctgctt atcgggcgcg cccgactatc ctttcccgcg cggtttcggc
     3181 cgtgcggttt tttgttgccg gtgccgtccg aagggggcag cggctttttg cgcggtcatt
     3241 cccgcctttc gttattcccg ccactttccg tcattcccgc gaaggcggga atcccgaaac
     3301 ccctgccgcc gtcattcccg cgcaggcggg aatccagtcc gttcggtttc agccgtttcc
     3361 gatagattcc tgccgcgttt ggggtctgga ttcccgcctg cgcgggaatg acggtggcgg
     3421 ggggtcggtt ttttccgata gattcctgcc gcgtttgggg tctggattcc cgcctgcgcg
     3481 ggaatgacgg cggcggggat ttctgttttt tccgatagat tcctgttgcg tcggggtctg
     3541 gattcccgct ttcgcgggaa tgacggcagc gggggtttct gttttttccg atagattcct
     3601 gccgcgtttg gggtctagat tcccgctttc gcgggaatga cggtggcggg ggtttcggtt
     3661 tttccgatag attcctgttg cgtcgggggg tctggattcc cgctttcgcg ggaatgacga
     3721 aagcggcggg aatgacgaag agtggcagga ttggcggggc ataggtttcg ggggattggc
     3781 ggcgcggccg gtttcggtcg gattgcttgg atcggcgggc tgaagcccat ctctacaact
     3841 cgtctctaca acccatctct acaacccatc tctacagcca tctctacagc catctctaca
     3901 acccatctct acaacccgtc tctacagcca tctctacagc ccatctctac agcc
//
LOCUS       DAWHRA010000155         3859 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWHRA010000155 DAWHRA010000000
VERSION     DAWHRA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.155"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WIB30_001891"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WIB30_001891"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ3797901.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WIB30_001892"
     CDS             1439..1924
                     /locus_tag="WIB30_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ3797902.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIB30_001893"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIB30_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ3797903.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          784 a          945 c         1076 g         1054 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgccga tgtcaaacct
      661 gaggacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggcgcg
      721 ataccgcgtt gggtgttgtt aagtgcggct ttgcccgaat tggatgaggt atggctggaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcattgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgt ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gcttgctcaa
     3121 caagagtttg cacaaagcga cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc agcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacccgccg caataatcgc ttccaaacgc ccctgctctt ccgccaacgc
     3301 gtcaaaatcc gcatcgggat tggcgtactc ggcatacact tcctccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcacgc acggtttttt ccggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aataccgccc atcggcacgg cttcgccctc
     3481 aaattcttta tccacgcccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcaaa ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg caccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAWHRA010000156         3853 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWHRA010000156 DAWHRA010000000
VERSION     DAWHRA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.156"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            1..643
                     /locus_tag="WIB30_001894"
                     /pseudo
     CDS             1..643
                     /locus_tag="WIB30_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            887..1543
                     /locus_tag="WIB30_001895"
     CDS             887..1543
                     /locus_tag="WIB30_001895"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ3797904.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSDSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRASLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            1698..3383
                     /gene="rpsA"
                     /locus_tag="WIB30_001896"
     CDS             1698..3383
                     /gene="rpsA"
                     /locus_tag="WIB30_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ3797905.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGNEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            3394..3708
                     /locus_tag="WIB30_001897"
     CDS             3394..3708
                     /locus_tag="WIB30_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ3797906.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
BASE COUNT         1027 a         1040 c          932 g          854 t
ORIGIN      
        1 tcggcgtgat gattaccttg tccagctatt tggataaaaa cgaaaatctg gttcagacgg
       61 cagttatcac ggcaattacc aataccatca tcgccatact tgcgggcttt atgattttcc
      121 cgtcgctctt cagcttcggc gttgcccccg attccggccc gactttggtg ttccaaagct
      181 tgccgattgt gttctcacat atgtgggcgg gatctgtgtt cgccgtgatt ttcttctcgc
      241 tgctcctgat tgccgcgttg acaacttcgc tgaccattta tgaagtgttg attacgacca
      301 ttcaggaaaa aaccaaaatc cgccgtaccg ccgcgattac gattgtattg gctgccatct
      361 tcattttcgg caacatcccg tccattctga gctatggtcc gtggaaagac gttccgtgtt
      421 cggcaaaaat attttcgatg ccttcgacta catcagcggc aacatcttgt ttatgctgac
      481 cgcgctcggt tccgcgctgt ttgccggttt tgtgatgaag gacgaagcga aggacgaatt
      541 gctttataaa ggcaaccata cgacggtcaa tatttggttt gcttatgtga aatatcttgt
      601 gccgctggtg attctgctga ttttcgtcag caacctgttc taatccgcag caatcgatgc
      661 cgtctgaagg tcatacctct ttcagacagc attgccttga tgcccgccac aatacccgca
      721 acgccgaaat ccgaaccggc ttgccgactt ctgtcggatg tttccgggca ggcggcgttc
      781 cgtctgctta gacaatcgtc ctttaaaaca ggtagaatcc gccccaatgg gaaatacatc
      841 cttcagacgg caaaacccat accccaaacc atcaggaatc ccccttatga acagacaaaa
      901 agtcatcgcc atcgacggcc cgggcgcatc gggcaaaggc acggttgccg cccgcgttgc
      961 cgccgcattg ggatacgatt atctcgatac cggcgcactc taccgcctga ccgccctata
     1021 tgcacaaaaa caaggcgtgg gatggcacga tgaagaaaac gtttccgaac tggcaaaaaa
     1081 actgcccgcc gtattttcag acagccgcat cctgctcggc ggcgaagacg tttcagacgg
     1141 catccggaca gaagccatcg gcatgggcgc atccgcagtc gcacagttgc ctaaagtccg
     1201 cgcctccctg ctgcaacgcc aacgcgattt tctgaccgaa aaaggactgg tcgccgacgg
     1261 acgggacacc ggatcggtcg tcttccccca agccgaactt aaaatcttcc tgacggcaga
     1321 atccaaaatc cgtgccgaac gccgcgccaa acaaatcggc atcccctgcg aaggtttggc
     1381 attcgagcgc atcctgtccg acatcgaagc cagagacgag gcagaccgaa accgcaaggt
     1441 tgcccccctg aaacaacaac ccgatgccct gcttttggat acgagccgcc tgactataga
     1501 agaaactgta aaaaaagtgc ttgattggta tcgtaaagtt taaattttca ggtataatcg
     1561 cccgcattgc gtttcagaca gcatgaattt ccccatatgc cgtctgaaac cttatttacc
     1621 cgtctgccct gccaaggacg gcagacaccc accaacccac cccgcacccc ttggcggtgt
     1681 accgaaaaga gttatatatg tctatggaaa attttgctca gctgttggaa gaaagcttta
     1741 ccctgcaaga aatgaacccg ggtgaggtga ttaccgctga agtagtggca atcgaccaaa
     1801 acttcgttac cgtaaacgca ggtctgaaat cagaatccct gattgatgta gctgaattca
     1861 aaaacgctca aggcgaaatt gaagttaaag tcggcgactt cgttaccgtt accatcgaat
     1921 ccgtcgaaaa cggcttcggc gaaaccaaac tgtcccgcga aaaagccaaa cgcgctgccg
     1981 attggattgc cctggaagaa gccatggaaa acggcaacat cctgtccggc atcatcaacg
     2041 gcaaagtcaa aggcggcctg accgttatga ttagcagcat ccgcgcattc ctgccgggtt
     2101 ctttggtcga cgtacgtcct gtaaaagaca cttctcactt cgaaggcaaa gagatcgaat
     2161 tcaaagtgat caaactggac aaaaaacgca acaacgtcgt tgtttcccgc cgcgccgttc
     2221 tggaagccac tttgggtgaa gaacgcaaag ccctgctgga aaacctgcaa gaaggatccg
     2281 tcatcaaagg catcgttaaa aacattaccg attacggtgc attcgttgac ttgggcggca
     2341 tcgacggtct gttgcacatc accgatttgg catggcggcg cgtgaaacac ccgagcgaag
     2401 tcttggaagt cggtcaggaa gttgaagcca aagtattgaa attcgaccaa gaaaaacaac
     2461 gcgtttcctt gggtatgaaa caactgggcg aagatccttg gagcggtctg acccgccgtt
     2521 atcctcaagg cacccgcctg ttcggcaaag tatccaacct gaccgactac ggcgcattcg
     2581 tcgaaatcga acaaggcatc gaaggtttgg tacacgtctc cgaaatggac tggaccaaca
     2641 aaaacgtaca cccgagcaaa gtcgtacaac tgggcgacga agtcgaagtc atgattttgg
     2701 aaatcgacga aggtcgccgc cgtatctctt tgggtatgaa acaatgccaa gccaatcctt
     2761 gggaagaatt tgccgccaac cacaacaaag gcgacaaaat ctccggcgcg gttaaatcca
     2821 ttaccgattt cggcgtattc gtcggcctgc ccggcggcat cgacggtttg gttcacctgt
     2881 ccgacctgtc ctggaccgaa tccggcgaag aagccgtacg caaatacaaa aaaggcgaag
     2941 aagtcgaagc cgtcgtattg gcaatcgacg tggaaaaaga acgcatctcc ttgggtatca
     3001 aacaactgga aggcgatccg ttcggcaact tcatcagcgt gaacgacaaa ggttctttgg
     3061 ttaaaggttc cgtgaaatct gttgacgcca aaggtgctgt tatcgccctg tctgacgaag
     3121 tagaaggcta cctgcctgct tccgaatttg cagccgaccg cgttgaagat ttgaccacca
     3181 aactgaaaga aggcaacgaa gttgaagccg tcatcgttac cgttgaccgc aaaaaccgca
     3241 gcatcaaact ttccgttaaa gccaaagatg ccaaagaaag ccgcgaagca ctgaactccg
     3301 tcaatgccgc cgccaatgcg aatgccggca ccaccagctt gggcgacctg ctgaaagcca
     3361 aactctccgg cgaacaagaa taaggttgca gacatgacaa agtctgagtt aatggttcgt
     3421 ttggcagaag tgtttgccgc caaaaacggc acgcatcttc tggcaaaaga cgtagagtac
     3481 agcgtaaaag tcttggttga caccatgact agatcgcttg cccgaggtca acgcatcgaa
     3541 atccgcggtt tcggcagctt cgatttgaac catcgtcctg cccgcatcgg tcgcaatccc
     3601 aaaaccggcg agcgtgtgga agtacctgaa aaacatgtac cccacttcaa gcccggtaaa
     3661 gaattgcgcg agcgggtcga cttggcttta aaagaaaatg ccaattaaac cttagcatca
     3721 aaacgccgct gttacgcggc gttttttctg tggtttaact tcatccgttg cttcaatacc
     3781 ttgagccaag caagcaacgg attagagcgt ggattttttt atagtggatt aacaaaaacc
     3841 agtacggcgt tgc
//
LOCUS       DAWHRA010000157         3771 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWHRA010000157 DAWHRA010000000
VERSION     DAWHRA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.157"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            374..3577
                     /gene="nalP"
                     /locus_tag="WIB30_001898"
     CDS             374..3577
                     /gene="nalP"
                     /locus_tag="WIB30_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ3797907.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGTSAPDFNAGG
                     TGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLHT
                     GDFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNE
                     NYKNYTAYMRKEAPEDGDGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIG
                     GRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTT
                     SRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFI
                     FSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAP
                     YEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAV
                     GVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGS
                     QLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIVYL
                     ADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGYL
                     NRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNA
                     ARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGI
                     RPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDHN
                     ATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTWSE
                     NSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLM
                     QLGALGGVNVPFSATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAG
                     LKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGL
                     GADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1024 a         1028 c         1000 g          719 t
ORIGIN      
        1 cgaagccgca gacagtacaa atagtacgga accgattcac ttgttaaaga atcgttctct
       61 ttgagctaag gcgaggcaac gccgtactgg tttttgttca tccactataa ctagggaaat
      121 tcaaattaag ttagaattat ccctatgaga aaaagccgtc taagccggta taaacaaaat
      181 aaactcattg agctatttgt cgaaagttca aatttccatt ttaaaacaat tagtaaaatc
      241 gagtttatcc tagttgtcca agacaacccc tataataata taattcaaaa tataaaaatg
      301 ggttacatct aaacattacg gaatttttat tccctcgcct gaattctatt gtcagattca
      361 aggagacctc atcatgcgaa cgaccccaac cttccctaca aaaactttca aaccggctgc
      421 catggcgtta gctgttgcaa caacactttc tgcctgctta ggcggcggcg gcggcacttc
      481 tgcgcccgac ttcaatgcag gcggcaccgg tatcggcagc aacagcaggg caacaacagc
      541 gaaatcagca gcagtatctt acgccggtat caagaacgaa atgtgcaaag acagaagcat
      601 gctctgtgcc ggtcgggatg acgttgcggt tacagacagg gatgccaaaa tcaatgcccc
      661 ccccccgaat ctgcataccg gagactttac aaacccaaat gacgcataca agaatttgat
      721 caacctcaaa cctgcaattg aagcaggcta tacaggacgc ggggtagagg taggtatcgt
      781 cgacacaggc gaatccgtcg gcagcatatc ctttcccgaa ctgtatggca gaaaagaaca
      841 cggctataac gaaaattaca aaaactatac ggcgtatatg cggaaggaag cgcctgaaga
      901 cggagacggt aaagacattg aagcttcttt cgacgatgag gccgttatag agactgaagc
      961 aaagccgacg gatatccgcc acgtaaaaga aatcggacac atcgatgtgg tctcccatat
     1021 tattggcggg cgttccgtgg acggcagacc tgcaggcggt attgcgcccg atgcgacgct
     1081 acacataatg aatacgcatg atggaaccaa gaacgaaata atgtctgcag ccatccgcaa
     1141 tgcatgggtc aagctgggcg aacgtggcgt gcgcatcgtc aataacagtt ttggaacaac
     1201 atcgagggca ggcactgccg accatttcca aatagccaat tcggaggagc agtaccgcca
     1261 agcgttgctc gcctattccg gcggtgataa aacagacgag ggtatccgcc tgatgcaaca
     1321 gagcgattac ggcaacttgt cctaccacat ccgtaataaa aacatgcttt tcattttttc
     1381 ggcaagcaat gacgcacaag ctcagcccaa cacactgacc ctattgccat tttatgaaaa
     1441 agatgctcaa aaaggcatta tcacagtcgc aggcgtagac cgcagtggag aaaagttcaa
     1501 tggctccaac cattgcggaa ttactgccat gtggtgccta tcggcaccct atgaagcaag
     1561 cgtccgtttc acccgtacaa acccgattca aattgccgga acatcctttt ccgcacccat
     1621 cgtaaccggc acggcggctc tgctgctgca gaaatacccg tggatgagca acgacaacct
     1681 gcgtaccacg ctgctgacaa cggctcagga catcggtgca gtcggcgtgg acagcaagtt
     1741 cggctgggga ctgctggatg cgggtaaggc catgaacgga cccgcgtcct ttccgttcgg
     1801 cgactttacc gccgatacga aaggtacatc cgatattgcc tactccttcc gtaacgacat
     1861 ttcaggcacg ggcggcctga tcaaaaaagg cggcagccaa ctgcaactgc acggcaacaa
     1921 cacctatacg ggcaaaacca ttatcgaagg cggttcgctg gtgttgtacg gcaacaacaa
     1981 atcggatatg cgcgtcgaaa ccaaaggtgc gctgatttat aacggggcgg catccggcgg
     2041 tagcctgaac agcgacggca ttgtctatct ggcagatacc gaccgatccg gcgcaaacga
     2101 aaccgtgcac atcaaaggcg atctgcagct gggcggcgaa ggtacgctgt acacacgttt
     2161 gggcaaactg ctgaaagtgg acggtacggc gatgaccggc ggcaagctgt acatgtcggc
     2221 acgcggcaaa ggggcaggct atctcaaccg taccggacaa cgtgttccct tcctgagtgc
     2281 cgccaaaatc gggcgggatt attctttctt cacaaacatc gaaaccgacg gtggtctgct
     2341 ggcttccctc gacagcgtcg aaaaaacagc gggcagtgaa ggcgacacgc tgtcctatta
     2401 tgtccgtcgc ggcaatgcgg cacggactgc ttcggcagcg gcacattccg cgcccgccgg
     2461 tctgaaacac gccgtagaac agggcggcag caatctggaa aacctgatgg tcgaactgga
     2521 tgcctccgaa tcatccgcaa cacccgagac ggttgaaact gcggccgccg accgcacaga
     2581 tatgccgggc atccgcccct acggcgcaac tttccgcgca gcggcagccg tacagcatgc
     2641 gaatgccgcc gacggtgtac gcatcttcaa cagtctcgcc gctaccgtct atgccgacag
     2701 taccgccgcc catgccgata tgcagggacg ccggctgaaa gccgtatcgg acgggttgga
     2761 ccacaacgct acgggtctgc gcgtcatcgc gcaaacccaa caggacggtg gaacgtggga
     2821 acagggcggt gttgaaggca aaatgcgcgg cagtacccaa accgtcggca ttgccgcgaa
     2881 aaccggcgaa aatacgacag cagccgccac actgggcatg ggacacagca catggagcga
     2941 aaacagtgca aatgcaaaaa ccgacagcat tagtctgttt gcaggcatac ggcacgatgc
     3001 gggcgatatc ggctatctca aaggcctgtt ctcctacgga cgctacaaaa acagcatcag
     3061 ccgcagcacc ggtgcggacg aacatgcgga aggcagcgtc aacggcacgc tgatgcagct
     3121 gggcgcactg ggcggtgtca acgttccgtt ttccgcaacg ggagatttga cggtcgaagg
     3181 cggtctgcgc tacgacctgc tcaaacagga tgcattcgcc gaaaaaggca gtgctttggg
     3241 ctggagcggc aacagcctca ctgaaggcac actggtcgga ctcgcgggtc tgaagctgtc
     3301 gcaacccttg agcgataaag ccgtcctgtt tgcaacggcg ggcgtggaac gcgacctgaa
     3361 cggacgcgac tacacggtaa cgggcggctt taccggcgcg actgcagcaa ccggcaagac
     3421 gggggcacgc aatatgccgc acacccgcct ggttgccggt ctgggcgcgg atgtcgaatt
     3481 cggcaacggc tggaacggct tggcacgtta cagctacgcc ggttccaaac agtacggcaa
     3541 ccacagcgga cgagtcggcg taggctaccg gttctgacgg acaggaagca gacagccgca
     3601 aagatcacgg tctttgcggc tgtttcttat gaaaagaaaa ccctattcca attgcctgct
     3661 tctattgttt caagacttct tccaaagatt cggcatcaat cagatgtata gcggattaac
     3721 aaaaatcagg acaaggcggc gaagccgcag acagtacaga accgattcac t
//
LOCUS       DAWHRA010000158         3633 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWHRA010000158 DAWHRA010000000
VERSION     DAWHRA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.158"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            49..867
                     /gene="opc"
                     /locus_tag="WIB30_001899"
                     /gene_synonym="opcA"
     CDS             49..867
                     /gene="opc"
                     /locus_tag="WIB30_001899"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ3797908.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWSAKV
                     HADLLSQLGNGWYINPWSEVKFDLKSRYKLNTGVTSLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVPTKSGDVSLTIPKTSIREYGLRVGIKF"
     gene            1030..1674
                     /locus_tag="WIB30_001900"
     CDS             1030..1674
                     /locus_tag="WIB30_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ3797909.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            1693..3603
                     /locus_tag="WIB30_001901"
     CDS             1693..3603
                     /locus_tag="WIB30_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3797910.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSREDKIQTASAPKASDAEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINTQLSYPEIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKRNGNAV"
BASE COUNT          986 a          924 c          845 g          878 t
ORIGIN      
        1 gttatgttac catatccgac tacaatccaa attttggaga ttttaactat gaaaaaaaca
       61 gtttttacat gtgccatgat tgccctgacc ggtactgccg ccgctgcaca agagcttcaa
      121 accgctaatg agtttaccgt ccacaccgac ctctcttcca tttcttcaac tcgtgctttc
      181 ctgaaagaaa aacacaaagc tgccaaacac atcggcgtac gtgctgatat tccttttgat
      241 gccaaccaag gcatccgctt ggaagccggt ttcgggcgca gcaaaaaaaa tattattaat
      301 ttggaaacag atgagaacaa gctgggtaag actaaaaatg taaaactgcc caccggcgtt
      361 cctgaaaacc gtatcgatct ttacacaggc tacacctaca cccaaacgtt aagtgattct
      421 ttaaatttcc gtgtgggtgc cggcttgggt tttgaatctt caaaagacag cattaaaacc
      481 accaagcata cgcttcacag cagccgtcag tcgtggtcag ccaaagttca cgcggatttg
      541 ctttcccaac tgggtaacgg ctggtatatc aacccttggt ctgaagtgaa atttgacctc
      601 aagtcccgct ataaattaaa caccggcgtt accagtctca aaaaagacat caatcaaaaa
      661 accaacggct ggggctttgg attgggtgca aatattggta aaaaactggg cgaatccgcc
      721 agcatcgagg cggggccgtt ctacaaacaa cgtacttaca aagaatccgg cgagtttagt
      781 gtaccaacca agagtggcga cgtatcgctc accatcccga aaaccagtat tcgtgaatac
      841 ggcttgcgcg tcggcataaa attctgatga tttgaaatca tcattgtcgc tttaaatgcc
      901 aaaccgcaaa ctattttggt ttgcggtttt tacgtgaaat gaatttgaat agccgatgcc
      961 gtctgaaaat ttcagacggc atttttccaa tcggttaaaa tacgcggttt gtttttttag
     1021 gaacgcgccg tgcttgcctc cgccatcgac ttcatcctcc atatcgacca acacctgctc
     1081 gcgctgtcgg cgcaatacgg tgtgtggatt tatgcgattc tgtttttgat tgttttttgc
     1141 gaaaccggcc tgattgttac gcccttactg cccggcgatt cgctgctgtt tgcggccggc
     1201 ggtattgccg cgctgggcgg tatggatatt catttgatgg ttgcattatt aagccttgcc
     1261 gccatactcg gcgacgcgct caattttacc gtcggcaaat atttcggcgg caggctgttc
     1321 gccaaccccg attctaaaat cttccgacgt gaatatctcg ataaaaccca ccgtttttac
     1381 gaaaaacacg gcggcaaaac catcatcatt gcccgtttta tgcccatcgt ccgcaccttc
     1441 gcccccttcg tcgccggtat ggggaaaatg cactatgcca aatttatccg ctacaacatc
     1501 atcggtggct tgttgtgggt tatcctgttt tcttacgcgg gctatttctt tgccaacttc
     1561 cccgtcgtaa aaaacaatct gggcttggtg atgggcggca tcattattgt ttctgtcctg
     1621 ccgggtgcgg ttgaaattgc ccgcgccaaa cttgcggtaa aatccaaacg gtaactgttg
     1681 agaatttgta tcatgaatat attatctgta gaaaacgctt cttttgccat cggccacgtt
     1741 gccttgctcg acaaaacttc ttttcaactc gacagcggcg aaaaagtcgg cttaatcgga
     1801 cgcaacggcg caggcaagtc ttcgttttta aaaatcctcg ccggtttgca aaagctcgac
     1861 gacgggcaga ttattgttca aaacaacctc aaaatcgttt atgtaccgca ggaatccttt
     1921 tttgataagg acgcaaccgt atttgatacc gttgccgaag gtttgggcga aattcgtgat
     1981 ttattgcgcc gttatcatca tgtcagccat gagttggaaa atggttcgag tgaggctttg
     2041 ttgaaagaac tcaacgaatt gcaacttgaa atcgaagcga aggacggctg gaaactggat
     2101 gcggcagtca agcagacttt gggggaactc ggtttgccgg aaaatgaaaa aatcggcaac
     2161 ctttccggcg gtcagaaaaa gcgcgtcgcc ttggctcagg cttgggtgca aaagcccgac
     2221 gtattgctgc tggacgagcc gaccaaccat ttggatatcg acgcgattat ttggctggaa
     2281 aatctgctca aagcgtttga aggcagcttg gttgtgatta cccacgaccg ccgttttttg
     2341 gacaatatcg ccacgcggat tgtcgaactc gatcgcggta ttttgcgttc ctatcccggc
     2401 tcgttctcta aatacagcga gaaaaaagcg caagagttgg cagtcgaagc ggaacacaac
     2461 cgcctctttg acaaattcca cgcacaggaa gaagcatgga tacgcaaagg catcgaagcg
     2521 cgccgtaccc gcaacgaagg ccgcgtgcgc cgtttggaag agctgcgccg ccagcgtgca
     2581 gaacgccgca acgtacaggg acaagtcaac ttcaagctcg atagcggtga gaaaagtggc
     2641 aaaatcatcg ccgaattgga acacgcttcg tttgcctatg gcggcaaagt cattatggac
     2701 aaattctccg ctatcttgca gcgcggcgac aaaatcggcc taatcggccc caacggcatc
     2761 ggcaaaacca ccttccttaa gctgattttg ggcgaattgc agccgaccta cggcagaatc
     2821 cgtatcggca gtaagcagga agtcgcctat ttcgaccagt tccgcagcgc gttgaatgaa
     2881 aacgacaccg tgttttacac cctcggacag ggaaacgatt acgttgaagt cggcggtaag
     2941 aaaaaacacg tcatgagcta tttggaagat ttcctgttcc atccagcccg tgcgcaaagc
     3001 cctgtttcat cgctctccgg cggagaacgc aaccgccttt tgctggcaaa actctttacc
     3061 cgtcctgcca atatcctggt cttggacgaa ccgaccaacg acttagacat cgacacccaa
     3121 gagctgctcg aagacctgtt gcgcgattat caaggcacag tattccttgt ctcgcacgac
     3181 cgtatgttcc ttgataacgt aattacccaa agcattgttt tcgaaggaca aggccgtctg
     3241 aaagaataca tcggcggcta tcaggactat atcgacgcaa aatcgcggga agataaaatt
     3301 cagacggcat ccgcacccaa agcatccgat gccgaacccg ccaaagaaaa acccaaagcc
     3361 aaccgcacgg tcaaactgtc ctacaaagag cagcgcgaac tcgatgccct gcccgacgaa
     3421 atcgccgctt tggaagccga gcaggctgaa atcaataccc aactttccta tcctgaaatt
     3481 ttcaaagatt acgaaaaagc aggtgcattg caaaaccgcg ccgaagaaat cgaaatgctg
     3541 cttttggaaa agctggaacg ctgggagctg ctggaaacca aacggaacgg caatgccgtc
     3601 tgaaatcaaa caggaagaaa aaaatgttca aac
//
LOCUS       DAWHRA010000159         3485 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWHRA010000159 DAWHRA010000000
VERSION     DAWHRA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.159"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..160
                     /locus_tag="WIB30_001902"
     CDS             <1..160
                     /locus_tag="WIB30_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797911.1"
                     /translation="ALKFDQLILEFPERGDGAWVHIGFRRNSPQRNQILTATKKNGKT
                     VYLPGLHP"
     gene            172..681
                     /locus_tag="WIB30_001903"
     CDS             172..681
                     /locus_tag="WIB30_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797912.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRSRNLPATTTAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAISSQRAE"
     gene            complement(734..1081)
                     /locus_tag="WIB30_001904"
     CDS             complement(734..1081)
                     /locus_tag="WIB30_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ3797913.1"
                     /translation="MYIPDKGDIFHLNFDPSSGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSSGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(1083..1319)
                     /locus_tag="WIB30_001905"
     CDS             complement(1083..1319)
                     /locus_tag="WIB30_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEZ3797914.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDVGNEVV"
     gene            complement(1678..2169)
                     /locus_tag="WIB30_001906"
     CDS             complement(1678..2169)
                     /locus_tag="WIB30_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ3797915.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLNVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACAIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(2421..2591)
                     /locus_tag="WIB30_001907"
     CDS             complement(2421..2591)
                     /locus_tag="WIB30_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797916.1"
                     /translation="MPSEIQTAFLLKGFPSTALHKGGFFMPSESLSDGIGLHGRSPPA
                     FKQERMSRMPDF"
     gene            complement(2607..2975)
                     /locus_tag="WIB30_001908"
     CDS             complement(2607..2975)
                     /locus_tag="WIB30_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEZ3797917.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMARLDGALSQIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
BASE COUNT          875 a          862 c          791 g          957 t
ORIGIN      
        1 tgcgctgaaa ttcgaccagt tgatactcga atttcctgag cgcggggacg gcgcatgggt
       61 acacatcggt ttccgacgca acagcccgca acgcaaccag atactgaccg caaccaagaa
      121 aaacggcaaa accgtgtatc tgcccgggct gcatccttga ggtcgtctga aatggatatt
      181 ttattgaaat actggaagcc ggtaggtgta ttgctgctaa tcgtcctgat ttttaccgca
      241 tggcatttcg accgtgccga aaaataccgc atgggacggg aggctgctgc tgccgaaatc
      301 tcgaatcgtc tgaaagacgg ctatatcgag caggcaaagc aggcgcgttc tgccgagcag
      361 aaggccgctg ccgcgtttgc cgaacgacaa accaaattag aagaggaaaa acaaaatgct
      421 gaaaaaactg ttgccgctat gcgtcttgag cttaaccgcc tgcgccacta cgccgccgcc
      481 caaaatcgca gcagaaacct gcccgcaacc actaccgccg ccaccgcatc tgatggcgcg
      541 tcagattccc aaggctggct attattcgga cagtgcgctg aaaaatatgc aggactggca
      601 gaaacagccg acggacatgc ggccgattta agggaatggc aggcatatgg tcatgcaata
      661 agcagtcagc gtgccgaata agcaaccgcc ggaacctgta atcttttctt acaggttcgg
      721 gcggtttcgg catttaatcg aataagacgg cgccgatgcg cgccagcaca tcgtccaata
      781 cataatcggg tacagtttct ttaaaagaag ccttgcggat ttgccagtcc agagatttga
      841 gctggtggca gtggacattg ccctgcgttt ccgttcctgc accgagtaag gttgaaatca
      901 tgccgctgct tcgtgcagcc gctgcattcc cctgtgaaat ggggcaggca aaaaccaatc
      961 ccgttgcgcg gttgaatgct tttggagaca gagccagcgc aaaccgcccg cccttgattt
     1021 ccttgccgct ggaagggtcg aaattcaaat ggaaaatatc gcctttgtcg ggaatataca
     1081 tttcagacga cctcgttgcc gacatcatcc aagatttccc agccttctac gcgcggcggg
     1141 gtttcttcca tttcggcaag caagtctgcc aagcggaaac gtcgggcagc acgcacacgg
     1201 agttcgccgt tatgtacttc cgctaccaaa gcgtcgccga ttttaaaatc caattgtttc
     1261 agcatgtcgg caggcagtcg gacggccgcc gagttccccc atttttggac acgcaacata
     1321 atcttcacct ttattgtatc tacaaagtag atacatatta ccataaaatt tcagttgttc
     1381 aaatacttgt gcagaatacg ccaaaagccg tccgaactgt ttcggacggc ttttgtactg
     1441 tatttgcgcc ttcaggcaat attttgttat ccattttcaa gatgcaaaag ctttctaatt
     1501 gcttgatgtc ggatttcggt tgtttaggga tacaaaacca agtaaactaa aactgtttga
     1561 ttggaaaatg ttccgcaagg agaaaattat gttcaaaaaa tcactttata aggctgcttt
     1621 ggcgtatttc ggcgattgcg tggctgccca tatttcagaa cagttttgac tgtttattca
     1681 caaatcagat gcctttaggg ggtttgcttt ccataatgca accaaaattt ccaactctct
     1741 aaatattgtg tctttgcgtt cttcttcgcg tattttcata acaagaggcg aaactgcatt
     1801 ccacaatttt atgaagttgg tgcaatgcag ccgtttaaac aaatcctcat ccaatactcc
     1861 cgagttgatt gcgcacgcat aaaactcgtg ccgattgatt acggtaagta aatgccctct
     1921 ctctttttcc cattgtccgc ccttattttg ataaatttca tacaggtcga tccgcgcgtt
     1981 gttgtcgtca actgccgtgc cgcgaatata aggcgaaata tgattgtcgg cttctacaaa
     2041 ttgtgcatct tggtaatcat tcaaaataac atttaatgtc gccttttgct ttgaagtttt
     2101 cttattgatg aatattgtcc ccagtgcaat gacggcggtt actgaaacaa cggtcagttg
     2161 ccaaaacatc agccattcgg ccgtccctca taggttaaac tgtacgctgt gcctgaatat
     2221 gtaaaataaa gtgaatacag atacaaacaa ggaggttagg gcaattactg acaaccaacc
     2281 tccttgtttt atttgtttcc gcaccttacc agccattaaa accctcattt ttgtatttca
     2341 tcgtatttcc ttaatggttt taaccttgct tgtcgatatc tgtatattaa tgccgtgcgc
     2401 ccgttctttc aaggggtgat ttaaaaatca ggcatccttg acatcctctc ctgcttaaag
     2461 gcggggggac tcctgccgtg taaaccaatg ccgtctgaaa ggctttcaga cggcataaaa
     2521 aaaccgcctt tgtgtaaagc ggttgaagga aagcctttca ataaaaatgc cgtctgaatt
     2581 tcagacggca ttgttgtcgg atatgcctat tccttatcca gccggcgcag ggtttcggcg
     2641 agctgtttga agtcggtttc tcccgccgcc aaactgacaa tcaagtcgtc aagcccttgg
     2701 tcggcggcaa tgctgatgcc ctgcaaatcc agataggtca acattgttaa aagcgcggtg
     2761 cgcttgttgc cgtcgggaaa ggcgtgggct ttggctatgg cttgtgcata gagggcggcg
     2821 atttcgtaga tgtcctcaag gttttcatac tgccgccagt tggcaatctg cgacaatgcg
     2881 ccgtccaagc gcgccatatc cgcccgccct ttcaaacccg cttcatccgc caatacggtt
     2941 tgatggataa gcgcgaccag ttcgccgtct atcatttgtc ggcaagtgcc ttgacggctt
     3001 tttgatgggt tttggcaatg cggcgggcgg cggcaagcag tacgcgtttg cctgcttcgc
     3061 ctttaagctc gattttgacg gggcgtgtat tttggttttg catcgctgct ccttgtctgt
     3121 ttgcgggcat tttagctttt ttccggcagc ttgggaaatg ccgtccgaaa acacttcaga
     3181 cggcattctt ctaatagtgt aatgcaatag ttactccaag atttttgtat atggctgttt
     3241 ttatggtaaa ttaaattttc ataagaaata aatatattta aatcaatgaa ataaaattta
     3301 gtcgaatccc accgtttccg ccacaaaaca aaaccgccct gattcggggc ggttcttttt
     3361 tgttcaagtt gagacctttg caaaattcct ttccttcccg acagccgaaa cccaaacaca
     3421 ggttttcagc tatttccgcc ccaaatacct cctaatttta ccccaatacc cccttaatcc
     3481 tcccc
//
LOCUS       DAWHRA010000160         3468 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWHRA010000160 DAWHRA010000000
VERSION     DAWHRA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.160"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            208..1680
                     /gene="pyk"
                     /locus_tag="WIB30_001909"
     CDS             208..1680
                     /gene="pyk"
                     /locus_tag="WIB30_001909"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ3797918.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVTAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1714..1941
                     /locus_tag="WIB30_001910"
     CDS             1714..1941
                     /locus_tag="WIB30_001910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797919.1"
                     /translation="MPEASDGIFCGGAAVRANSEKIVQIRQHRIKSSTYTKAKQGISD
                     CLFYSGLNLNQYSVASPCRTICTVCGFVALS"
     gene            1995..3395
                     /locus_tag="WIB30_001911"
     CDS             1995..3395
                     /locus_tag="WIB30_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ3797920.1"
                     /translation="MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          893 a         1047 c          882 g          646 t
ORIGIN      
        1 aaataaattt agcctaagtc ataaaaccgt gtaattttgc gccaaaaaag gcggtttgca
       61 ggtaaaatat caaactatta atttaaatca tatttttaca gaatattccg ccgcgttcat
      121 caaatggaca tcaaaccggt tccaaatttc tgtaattttg taacaaaata ccgcaaaaca
      181 cccgattgag accaaaagga ctttcatatg aaccagacaa gccgcgatct gacccgcatc
      241 agccacaaca ctaaaatcgt cgccaccctt gggccgggca gcaacaacgt cgaactgttg
      301 gaagacatga tccgcgtcgg cggtctgaac gtcgtccgct tcaacttcag ccacggcacg
      361 cccgaattcc atcaggaaaa cgcccgcatt gtgcgcgagg cggcaaaacg cgccggacag
      421 gaaatcgcca tcattgccga cctgcaaggg ccgaaaatcc gcgtgggcaa aatcgccggc
      481 ggcggcatcg aattgaacaa aggcgaaacg ctggtactgg atgccgcgct cgaaggcgaa
      541 ggcacgcgcg aggcggtcgg tttggactac cgcgacctgc ccgacgacgt taccgcaggc
      601 gacgtattgt ggctggacga cggcctgctg accctgaccg tggaatccgt cgaaggcagc
      661 aggattatca caagggtgga aaacagccac gtcctgaaaa gcaacaaggg catcaacaaa
      721 cgcggcggcg gtctatctgc aggcgcgttg accgaaaaag acttccgcga cctgaaaacc
      781 gcgattgcca tcggttgcga ctacctcgcc atcagctttg tgaaatccgc cgaagatttg
      841 catatcgcgc gcgccaaagt cgaagaagaa atgaagggca gcactgccgt gcgccccggt
      901 ttggtttcca aaatcgaacg cgtagaagcg attgaaaact tggacgaaat catcctcgcc
      961 ggcgacggca ttatggttgc gcgcggcgac ttggcggtcg aagtcggaca cgccgccgtc
     1021 cccgccctgc aaaaacggat gatccgccgc gcccgcgagt tgcgccgctt cagcattacg
     1081 gcgacgcaaa tgatggaatc gatgattacc aaccccgtac cgacccgcgc ggaagtcagc
     1141 gatgtggcaa acgcggtatt ggacggtacc gatgcggtga tgtgttccgc cgaaaccgcc
     1201 gtcggcgcgt atccgtttga aaccgtcagc caaatggcga ttatctgcgc ggctgcggaa
     1261 aaagagcagg attcgctcaa cggcgttgcc gaacaggtcg agtatcccga agcggtcagc
     1321 accaacctgg cggttgccgg cggtgcggtc agcgtggcgc gcgcggttca cgccaaagcc
     1381 atcgtcgccc tgaccgaaag cggttcgacc gcctttgaaa tcagccgcca caacatcacc
     1441 ctgccgattt tcgcgctgac cccgagcgtt tccgcccaac gccgtatggc gatgtaccgg
     1501 ggcgtgcgcc cgctgatttt ggcaaccagt accgaccacg acacggcgtt aaacgaagtc
     1561 gaaacgatgc tggtggaaca caacatcctg cattccggcg accaatacat cattaccagc
     1621 ggttcgcaaa tgcgcgaatc cggttcgacc aacacactgg aagtgctgcg cgtcaaataa
     1681 tccgccctga gtggaaaatg ccgtctgaag ccgatgcccg aggcttcaga cggcattttt
     1741 tgcggcggcg cggcggttcg ggcaaactct gaaaaaattg tgcaaatccg gcaacatcgg
     1801 ataaaatcga gtacctatac taaagcgaaa caaggcattt ccgactgcct tttttatagt
     1861 ggattaaatt taaaccagta cagcgttgcc tcgccttgcc gtactatctg tactgtctgc
     1921 ggcttcgtcg ccttgtcctg atttttgtta atccactata tttatccacc gtccgccttt
     1981 ttacggaaac cgaaatgacc ccttccgcac tgaaaaaaac cgtcctgctg ctcggcactg
     2041 cctttgccgc cgcatccgtc cacgcatccg gctaccactt cggcacacag tcggtcaacg
     2101 cgcaaagcac ggcaaatgcc gccgccgcag aagccgccga cgcatcgacc atcttctaca
     2161 accctgccgg cctgaccaaa ctcgacagca gccagatttc cgtcaacgcc aacatcgtgc
     2221 tgcccagcat tcattatgag gcggattccg ccaccgactt taccgggctt cccgtccaag
     2281 gttcgaaaag cggcaaaatc accaaaacca cggtcgcgcc ccacatctac ggcgcataca
     2341 aagtcaacga caatctgacc gtgggcttgg gcgtgtacgt ccccttcggc tctgccaccg
     2401 aatacgaaaa agattccgtg ttgcgccaca acatcaacaa actcggtctg accagcatcg
     2461 ccgtcgaacc tgtcgccgcg tggaaactca acgaccgcca ttccttcggc gcaggcatca
     2521 tcgcccaaca tacttccgcc gaactgcgca aatatgccga ctgggggatt aagagtaaag
     2581 cagagatatt gacggcaaaa ccgcccaaac ctaacggtgt agccgaagct gcaaaaattc
     2641 aggccgacgg acacgccgat gtcaaaggca gcgattgggg cttcggctac caactggcgt
     2701 ggatgtggga catcaacgac cgtgcgcgcg tgggcgtgaa ctaccgttcc aaagtctcgc
     2761 acacgctcaa aggcgatgcc gaatgggcgg cagacggcgc ggcggcgaaa gcaatgtgga
     2821 gtacgatgct tgcagcaaac ggctacacgg cgaatgaaaa agcccgcgtt aaaatcgtta
     2881 cgcctgagtc tttgtccgta cacggtatgt acaaagtgtc cgataaagcc gacctgttcg
     2941 gcgacgtaac ttggacgcgc cacagccgct tcgataaggc ggaactggtt tttgaaaaag
     3001 aaaaaaccgt cgtcaaaggc aaatccgacc gcaccaccat cacccccaac tggcgcaaca
     3061 cctacaaagt cggcttcggc ggttcttatc aaatcagcga accgctgcaa ctgcgcgccg
     3121 gcatcgcttt tgacaaatcg cccgtccgca acgccgacta ccgcatgaac agcctacccg
     3181 acggcaaccg catctggttc tccgccggta tgaaatacca tatcggtaaa aaccacgtcg
     3241 tcgatgccgc ctacacccac atccacatca acgacaccag ctaccgcacg gcgaaggcaa
     3301 gcggcaacga tgtggacagc aaaggcgcgt cttccgcacg tttcaaaaac cacgccgaca
     3361 tcatcggtct gcaatacacc tacaaattca aataaacggc ccgccgtttg aatgtaacaa
     3421 tgccgtctga aacagatttc cgtttcagac ggcattgttt taggctcg
//
LOCUS       DAWHRA010000161         3460 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWHRA010000161 DAWHRA010000000
VERSION     DAWHRA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.161"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            784..1107
                     /locus_tag="WIB30_001912"
     CDS             784..1107
                     /locus_tag="WIB30_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ3797921.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            1221..3398
                     /locus_tag="WIB30_001913"
     CDS             1221..3398
                     /locus_tag="WIB30_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ3797922.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQKSTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          850 a         1177 c          876 g          557 t
ORIGIN      
        1 gcttccttcg aaagctccgt tttcagacga ccttttggaa caccacaggc acacgcatcg
       61 aacggctgaa tcaaagattc agaccgatgg cagtccgcac ccgagtttat gcggcaaaca
      121 gcgaggctac ggcaacccgc cccctctccc tgtgggagag ggttagggag agggcagtaa
      181 gccgtaggct tacatcaaag ccgataacgc tttcacaaag cctgtgtgca ctgaaaactg
      241 aaaggtcgtc tgaaaacgga gctttcgaag gaagcagagt ttctgcgaag ctaaaaccga
      301 agctgcaaaa aatcgaaaag cagcctgcac gttgaaaatg ccgcccaagc aaacctgcgg
      361 tttgccgccc tctccctagc cctctcccac agggagaggg gattggggtg caggctgtct
      421 ttaagcttca ggcgattttt aactcttgtt gaagctgcga tttcagaagc tccgttttag
      481 cttcgcagaa actccgcttc cttcgaaagc tccgttttca gacgaccttt tggaacacca
      541 caggcacacg catcgaacgg ctgaatcaaa gttcagaccg atggcagccc gcacccgagt
      601 ttatgcggca aacagcgagg ctacggcaac ccgtcccctc tccctgtggg agagggttag
      661 ggagagggcg gtaagccgca ggcttgcatc aaagtcgata acgctttcgt tacaactccg
      721 cccactgaac gaagccaaaa cgacaaaccg catcgtaaac cacccaaccc ataggagaac
      781 cccatgcaaa acgaaaccat caacctgaaa cagcaccttg ccgccatcaa agaatactgg
      841 cagcccgaaa tcatcaaccg ccacgggttc caattccgtt tggtcaaact tttgggcgat
      901 tacggctggc atacgcacgg atacagcgac aaagtgctgt ttgccgtgga gggcgacatg
      961 gcggtggact tcgccgacgg cggcagcatg acgatacgcg agggcgagat ggcggtcgtg
     1021 ccgaagtcgg tgtcgcaccg cccgcgttcg gaaaacggct gctcgttggt gctgattgag
     1081 ttgtccgacc cgtccgaggc cgtctgaaaa cgaagtttcc gaaggaagct gagtttctgc
     1141 gaagctaaaa gcagcctgca ccttcaatca atatgccgaa aatacaaccc caccgcacac
     1201 caacacacaa aggaaatccc atgacacgct tcaaatattc cctgctgttt gccgccctgt
     1261 tgcccgtgta cgcgcaggcc gatgtttctg tttcagacga ccccaaaccg cagaaaagca
     1321 ctgaattgcc gaccatcacc gttaccgccg accgcaccgc gagttccaac gacggctaca
     1381 ctgtttccgg cacgcacacc ccgctcgggc tgcccatgac cctgcgcgaa atcccgcaga
     1441 gcgtcagcgt catcacatcg caacaaatgc gcgaccaaaa catcaaaacg ctcgaccgcg
     1501 ccctgttgca ggcgaccggc accagccgcc agatttacgg ctccgaccgc gcgggctaca
     1561 actacctgtt cgcgcgcggc agccgcatcg ccaactacca aatcaacggc atccccgttg
     1621 ccgacgcgct ggccgatacg ggcaatgcca acaccgccgc ctatgagcgc gtagaagtcg
     1681 tgcgcggcgt ggcggggctg ctggacggca cgggcgagcc ttccgccacc gtcaatctgg
     1741 tgcgcaaacg cccgacccgc aagccattgt ttgaagtccg cgccgaagcg ggcaaccgca
     1801 aacatttcgg gctgggcgcg gacgtatcgg gcagcctgaa caccgaaggc acgctgcgcg
     1861 gccgcctggt ttccaccttc ggacgcggcg actcgtggcg gcagcgcgaa cgtagccgcg
     1921 atgccgaact ctacggcatt ttggaatacg acatcgcacc gcaaacccgc gtccacgcag
     1981 gcatggacta ccagcaggcg aaagaaaccg ccgacgcgcc gctcagctac gccgtgtacg
     2041 acagccaagg ttatgccacc gccttcggcc cgaaagacaa ccccgccaca aattgggcga
     2101 acagccgcca ccgtgcgctc aacctgttcg ccggcatcga acaccgcttc aaccaagact
     2161 ggaaactcaa agccgaatac gactacaccc gcagccgctt ccgccagccc tacggcgtag
     2221 caggcgtgct ttccatcgac cacaacaccg ccgccaccga cctgattccc ggttattggc
     2281 acgccgaccc gcgcacccac agcgccagcg tgtcattgat cggcaaatac cgcctgttcg
     2341 gccgcgaaca cgatttaatc gcgggtatca acggttacaa atacgccagc aacaaatacg
     2401 gcgaacgcag catcatcccc aacgccattc ccaacgccta cgaattttcc cgcacgggtg
     2461 cctacccgca gcctgcatcg tttgcccaaa ccatcccgca atacggcacc aggcggcaaa
     2521 tcggcggcta tctcgccacc cgtttccgcg ccgccgacaa cctttcgctg atactcggcg
     2581 gcagatacag ccgttaccgc accggcagct acgacagccg cacacaaggc atgacctatg
     2641 tgtccgccaa ccgtttcacc ccctacacag gcatcgtgtt cgacctgacc ggcaacctgt
     2701 ctctttacgg ctcgtacagc agcctgttcg tcccgcaatc gcaaaaagac gaacacggca
     2761 gctacctgaa acccgtaacc ggcaacaatc tggaagccgg catcaaaggc gaatggcttg
     2821 aaggccgtct gaacgcatcc gccgccgtgt accgcgcccg taaaaacaac ctcgccaccg
     2881 cagcaggacg cgacccgagc ggcaacacct actaccgcgc cgccaaccaa gccaaaaccc
     2941 acggctggga aatcgaagtc ggcggccgca tcacgcccga atggcagata caggcaggtt
     3001 acagccaaag caaaacccgc gaccaagacg gcagccgcct gaaccccgac agcgtacccg
     3061 aacgcagctt caaactcttc actgcctacc actttgcccc cgaagccccc agcggctgga
     3121 ccatcggcgc aggcgtgcgc tggcagagcg aaacccacac cgaccctgcc acgctccgca
     3181 tccccaaccc cgccgccaaa gcccgcgccg ccgacaacag ccgccaaaaa gcctacgccg
     3241 tcgccgacat catggcgcgt taccgcttca atccgcgcgc cgaactgtcg ctgaacgtgg
     3301 acaatctgtt caacaaacac taccgcaccc agcccgaccg ccacagctac ggcgcactgc
     3361 ggacagtgaa cgcggcgttt acctatcggt ttaaataaga tcgtctgaaa acggagtttc
     3421 tgcgaagcta tagtggatta acaaaaacca gtacggcgtt
//
LOCUS       DAWHRA010000162         3451 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWHRA010000162 DAWHRA010000000
VERSION     DAWHRA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.162"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(45..1289)
                     /gene="clpX"
                     /locus_tag="WIB30_001914"
     CDS             complement(45..1289)
                     /gene="clpX"
                     /locus_tag="WIB30_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ3797923.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            1480..1851
                     /gene="rbfA"
                     /locus_tag="WIB30_001915"
     CDS             1480..1851
                     /gene="rbfA"
                     /locus_tag="WIB30_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ3797924.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            1884..2807
                     /gene="truB"
                     /locus_tag="WIB30_001916"
     CDS             1884..2807
                     /gene="truB"
                     /locus_tag="WIB30_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ3797925.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            3024..>3451
                     /locus_tag="WIB30_001917"
     CDS             3024..>3451
                     /locus_tag="WIB30_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797926.1"
                     /translation="MKTDIQTELTQVLLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLSNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDSNRFL
                     KDSSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPAS"
BASE COUNT          894 a          952 c          796 g          809 t
ORIGIN      
        1 cttcagaaac gtggttcaac gttccgaagc ggttttgttt gccatcagga ctcgaacacc
       61 aattccggtt ccctgccctc ttcgatgact gccttaccga ccaccacttt tttcaagcct
      121 ttcaaatcag gcaggcggta catcgtatcg agcaggcagc gttcgacgat ggacctcagg
      181 ccgcgcgcgc cggttttgcg ttccattgcc tgacgcgcga tggaacgcaa tgcgccttct
      241 tcaaactcca gttcgacatt ttccataccg aacaacgcct gatactgctt gaccaaagca
      301 tttttcggct cggtcaaaat attaatcagc gcgtcttcgt ccaattcttc taaagttgca
      361 atcacaggca aacgtccgat taattctgga atcagaccga atttaatcaa gtcttccggt
      421 tcgacgatgc cgaacagctt ggtaatgtcg gcattttcgt ccttgctgtg aacggacgca
      481 ccgaaaccga taccgccttt ttcagtacgc tggcggatga ctttttccaa gcctgcaaac
      541 gcgccgccgc agataaacag gatgttggtg gtatcgacgt tgataaattc ctgattcgga
      601 tgcttgcggc cgccttgggg cggaacgctg gccactgtac cttcaatcag tttcaacaag
      661 gcttgttgca caccttcgcc ggatacgtcg cgggtaatcg acgggttgtc gcttttgcgt
      721 gaaattttat cgatttcgtc gatatagaca atgccgcgct gggctttttc gacatcgaaa
      781 tcacatttgc ccaaaagctt ggtaatgatt tgctcgacgt cttcgccgac ataacctgct
      841 tcagtcaaag ttgtggcatc cgccatcacg aacggcacat ccagtttgcg cgccaaagat
      901 tgcgccagca gggttttacc cgatccggtc gggccgataa gcaggatgtt ggatttcgac
      961 aattcgacat tagctcctgc tttaggatgg cgcaggcgtt tgtaatggtt gtacaccgac
     1021 accgccaagg ctttcttggc ttgttcctga ccgataacgt ggtcgttgag gttggcgacg
     1081 atttcggcgg gcgtgggcag cttgccggat tcttccggct cccctccggc actttccgaa
     1141 ggcgtgccgt catttccgtc ttcatgcaat atttcaatac agtttgagac gcattcgtca
     1201 cagataaagg cgttttcgcc ctcaattaaa tgtttgacgt gtgatttgga ttttccgcaa
     1261 aaggaacaag tacggttttc gttggacatg gctttcttta caatgtatgc gttacagaaa
     1321 acggcacgtg ccgttcgggt tgccaagtat aataactata tccgttctta tcaatgtatt
     1381 accttaaaat cccgccgatt aggctataat acgccctttc gcaaccgccc cggcggcaaa
     1441 aatgccgtct gaaaccaaat ctgaaatctg aggatattca tgagaaaacc ccaacgcggc
     1501 tatgcccgcc aagaccgtgt caaagaacaa attatgcgcg agcttgccga actcgtccgt
     1561 accggactga aagacccgcg cgccggcttc attaccgtca acgaagtcga agttacccgc
     1621 gattacagcc acgccaccgt gttctacacc attttaaacc aagacgcgcg cgaaattacg
     1681 gaagaagtgc tggaacacgc gcgcggacac ctccgcagcg aattggccaa acgcatcaag
     1741 ctgttcaaaa cgcccgaact gcatttcaaa tacgacgaat ctttggaacg cggtttgaac
     1801 ctgtccgccc ttatcgacca agtagcggcg gaaaaaccgg ttgaagactg acggatatgc
     1861 ccatgccgtc cgaacatcga accatgaata caggcaaacc ccaaaaacgt gccgtcaacg
     1921 gtgttttgct cttggacaaa cccgaaggcc tttccagcaa caccgcgctg caaaaagcgc
     1981 ggcgtttgtt tcatgccgaa aaagccggac ataccggcgt gctcgaccct ttggcaaccg
     2041 gacttttgcc cgtctgcttc ggtgaagcga ccaagttcgc ccaatacctg ctggatgccg
     2101 acaaagccta caccgccacg ctgaaactcg gcgaagccag cagcacgggt gatgccgaag
     2161 gcgaaatcat tgccaccgcc cgcgccgata tttccttagc cgaatttcag acggcctgcc
     2221 aagcactgac aggcaacatc cgccaagtgc cgccaatgtt ttccgcgctc aagcacgaag
     2281 gcaaaccgct gtacgaatac gcccgcaaag gcatcgtcat cgaacgcaaa gcgcgctaca
     2341 ttaccgttta cgccatcgat attgccgaat ttgacgcgcc caaagccgtc atcgacgtac
     2401 gttgcagcaa aggcacctac atccgcaccc tcagcgaaga catcgccaaa cacatcggca
     2461 cgttcgccca cctgaccgcc ctgcgccgca ctgaaaccgc cggctttacc atcgcccaaa
     2521 gccacacgct tgaggccttg gcaaatttga acgaaacaga acgcgacagc ttgctgctac
     2581 cctgcgacgt attggtttca cactttcccc aaaccgtttt aaacgattat gccgtccata
     2641 tgctccactg cggacaacgt ccgcgtttcg aagaagacct gccttccgac acgccggtac
     2701 gcgtttacac ggaaaacggc cgctttgtcg gtctggcgga atatcaaaaa gaaatatgcc
     2761 gtctgaaagc cttgcgcctg atgaacacgg cggcatccgc cgcctgaacg gcggttaaaa
     2821 atacaggctg tgcttgaata atgtgttgat atttccgcaa aatcccgaca cactcggaca
     2881 cccgccccgc ttatcgcaac tttgcgaacg cccccggaaa cagcaaagac atcaaataat
     2941 tgattttatt agaatctatt tgcaaagcca tttgccgtta cacaagaatg gcacattaaa
     3001 ataactgatg aggatttata acgatgaaga cagacattca aaccgaatta acccaagtcc
     3061 tactaccaca cgaaaaagta tgggcgaacg aagaaaaaac cattttagcc aaaaacatcc
     3121 tgttggattt ggtggaaaaa accgacccga ccattatcgg tttgttattg agtaatgatg
     3181 agttaaaacg ccatttcttt gtggaagtga atggtgtgct ggtgtttaaa ttgcaggatt
     3241 tccgtttttt cttggacaaa cacagcgtca ataattccta cacaaaatac gccaaccgca
     3301 ttggtttgac ggacagcaac cgctttttga aagacagcag tgatattgtg ttggattttc
     3361 cgtttaaaga ttgtgtgtta aatggcggac aaagcaccga ggaaggcgaa gaaatttatt
     3421 ttaaacgcaa taacagccag ccagccagcc a
//
LOCUS       DAWHRA010000163         3443 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWHRA010000163 DAWHRA010000000
VERSION     DAWHRA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.163"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..499
                     /locus_tag="WIB30_001918"
     CDS             <1..499
                     /locus_tag="WIB30_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ3797927.1"
                     /translation="AAAAQKLISDAALYHPQQYEQIKTAALQQQQTETIYRLAEAQAC
                     AIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPRAAAHWYAIAAEQN
                     HPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLISLLEQWRREAHHAI
                     GQKAV"
     gene            717..1931
                     /gene="gltS"
                     /locus_tag="WIB30_001919"
     CDS             717..1931
                     /gene="gltS"
                     /locus_tag="WIB30_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ3797928.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            2030..3046
                     /locus_tag="WIB30_001920"
     CDS             2030..3046
                     /locus_tag="WIB30_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ3797929.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            3057..3254
                     /locus_tag="WIB30_001921"
     CDS             3057..3254
                     /locus_tag="WIB30_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797930.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            3272..3421
                     /locus_tag="WIB30_001922"
     CDS             3272..3421
                     /locus_tag="WIB30_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797931.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTHRTIQKTS
                     PYVPS"
BASE COUNT          806 a          926 c          945 g          766 t
ORIGIN      
        1 tgccgccgca gcacaaaaac tcatcagcga cgccgcgctg taccatccgc aacaatacga
       61 acaaatcaaa actgccgccc tgcaacaaca acaaaccgaa accatctacc gtttggcgga
      121 agcacaagcc tgcgccatcg gccgtcccgc cgactacaat gccgcgcgaa aaaattacat
      181 ggaagctgcc gggttccacc ataaaaacgc agcggcagcc ttaggccgca tctaccatta
      241 cggcctcggt acggcgcaag atcctcgggc ggctgcacac tggtacgcca ttgctgccga
      301 acaaaaccac ccttccgccc aataccacct cgcctgtttt tactatcacg ggcaaggtgt
      361 cggctgtcat gttccgaccg cctgctactg gctgcaggcc gccatcggca acggccacac
      421 ttcggccgaa tcattaatat ccctattaga acaatggcga cgcgaagcac accatgccat
      481 cggacaaaag gccgtctgaa aagatttaca ctcgcatttt ttgacaatct ttaactattc
      541 ccctaatatt tgccagttat ttttcacgga cacgccattg ttttcatttc tttctgaaaa
      601 caccttgtcc gcgcatcaat accatgacac tcggcggata acgccaagcg ttgaaacaca
      661 ctacatccgg aacaaaaacg gatgctcgga aaaatatttc taggaggtga aacaacatgg
      721 aatgggaatt caacagttat tacacactga ttgccgccac gctcgtgttg ctggttggta
      781 aatttctggt tcaaaaaatc aaattcttac gagacttcaa tattcccgag ccggtcgctg
      841 gcggtttggt tgccgcgatt atcctgtttg cgctgcacga ggcgtacggc gtgagcttca
      901 aatttgagaa accgctgcaa aatgcgttta tgctgatttt cttcacgtcc atcggcttga
      961 gcgcggattt ttcccgtttg aaggcgggcg gtttgccgct ggtggttttt accgcgattg
     1021 tgggcggatt tatcttggtg caaaactttg tcggggtcgg actggctacg gctttgggtt
     1081 tggacccgct catcggtctg attaccggtt cggtgtcgct gacgggcgga cacggtacgt
     1141 caggtgcgtg gggacctaat tttgaaacgc aatacggctt ggtcggcgca accggtttgg
     1201 gtattgcatc ggctactttc gggctggtgt tcggcggcct gatcggcggg ccggttgcgc
     1261 gccgcctgat caacaaaatg ggccgcaaac cggttgaaaa caaaaaacag gatcaggacg
     1321 acaacgcgga cgacgtgttc gagcaggcaa aacgcacccg cctgattacg gcggaatctg
     1381 ccgttgaaac gcttgccatg tttgccgcgt gtttggcgtt tgccgagatt atggacggct
     1441 tcgacaaaga atatctgttc gacctgccca aattcgtgtg gtgtctgttt ggcggcgtgg
     1501 tcatccgcaa catcctcact gccgcattca aggtcaatat gttcgaccgc gccatcgatg
     1561 tgttcggcaa tgcttcgctt tcgcttttct tggcaatggc gttgctgaat ttgaaactgt
     1621 gggagctgac cggtttggcg gggcctgtaa ccgtgattct tgccgtacaa accgtggtga
     1681 tggttttgta cgcgactttt gttacctatg tctttatggg gcgcgactat gatgcggcag
     1741 tattggctgc cggccattgc ggtttcggct tgggtgcaac gccgacggcg gtggcaaata
     1801 tgcagtccgt cacgcatact ttcggcgcgt cgcataaggc gtttttgatt gtgcctatgg
     1861 tcggcgcgtt cttcgtcgat ttgattaatg ccgcgattct caccggtttt gtgaatttct
     1921 ttaaaggctg attttccgcc tttccgccaa agcacctgca aggtttaccg cctgcaggtg
     1981 cttttgctat gatagccgct atcggtctgc accgtttgga aggaacatca tgtatcggaa
     2041 actcattgcg ctgccgtttg ccctgctgct tgccgcttgc ggcagggaag aaccgcccaa
     2101 ggcattggaa tgcgccaacc ccgccgtgtt gcaaggcata cgcggcaata ttcaggaaac
     2161 gctcacgcag gaagcgcgtt ctttcgcgcg cgaagacggc aggcagtttg tcgatgccga
     2221 caaaattatc gccgccgcct acggtttggc gttttctttg gaacacgctt cggaaacgca
     2281 ggaaggcggg cgcacgttct gtatcgccga tttgaacatt accgtgccgt ctgaaacgct
     2341 tgccgatgcc aaggcaaaca gccccctgct gtacggggaa accgctttgt cggatattgt
     2401 gcggcagaag acgggcggca atgtcgagtt taaagacggc gtattgacgg cagccgtccg
     2461 cttcctgccc gtcaaagacg gtcagacggc atttgtcgac aacacggtcg gtatggcggc
     2521 gcaaacgctg tctgccgcgc tgctgcctta cggcgtgaag agcatcgtga tgatagacgg
     2581 caaggcggtg aaaaaagaag acgcggtcag gattttgagc ggaaaagccc gtgaagaaga
     2641 accgtccaaa cccacgcccg aagacatttt ggaacacaat gccgccggcg gcgatgcggg
     2701 cgtaccccaa gccgcagaag gcgcgcccga accggaaatc ctgcatcctg acgacggcga
     2761 gcgtgccgat accgttaccg tatcacgggg cgaagtggaa gaggcgcgcg tacaaaacca
     2821 gcgtgcggaa tccgaaatta ccaaactttg gggaggactc gataccgacg tgcaaaaaga
     2881 gttggtcggc gaacaacgca agtgggcgca ggaaaaaatc agcaactgcc gacaagccgc
     2941 cgcgcaggca gaccggcagg aatacgccga atacctcaag ctgcaatgcg acacgcggat
     3001 gacgcgcgaa cggatacagt atcttcgcgg ctattccatc gattaggggc aaaccgatga
     3061 ctaccgtccc caaaagcagg attcccgtca aaccgctgcc cgaaaaaacc acagacgaag
     3121 ccaaagtcga aaaatggcgg cagctcggtg cggaacacgg tttgtcgggc gaatgggcag
     3181 ttgccgccag attgggcgag aacggtttta ccgaagaaca gatggaaaat atcgccaacc
     3241 tgttcggcag ataaagggaa aattgacgga aatgccgtct gaaaccctgt tatcggtttc
     3301 agacggcatt ttgactaata cggtacgcag gcgcaaaaca gccggctttt cctgtgttgc
     3361 ctatgctgat gtttcaacac acaggacgat acaaaaaacg tcgccctatg tgccgtcctg
     3421 attcggaaag ggttacgctc ctc
//
LOCUS       DAWHRA010000164         3442 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWHRA010000164 DAWHRA010000000
VERSION     DAWHRA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.164"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            43..708
                     /locus_tag="WIB30_001923"
     CDS             43..708
                     /locus_tag="WIB30_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ3797932.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTANNKCDSGKKEQGMLAFGDKNGNK
                     EYNDTADVLLRSVVLNDDINNKRINYAFNHIAFGHTQPTADRVVWTFNQNGTFGYTKD
                     RHLTKQSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRNDRRTVC
                     QYK"
     gene            738..1346
                     /gene="pilV"
                     /locus_tag="WIB30_001924"
     CDS             738..1346
                     /gene="pilV"
                     /locus_tag="WIB30_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ3797933.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHTLSAVNGEFAIDAI
                     KTKTQLAEEQLKRFSYELKNALPDAAVIHYAVCKDSSGNAPTLSGNAFSSNCDNKANG
                     DTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            1343..2341
                     /locus_tag="WIB30_001925"
     CDS             1343..2341
                     /locus_tag="WIB30_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ3797934.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAAQQDLRNAATLIVRDARMAGGFGCFNMSEHTKNDIIVDPSKQTQP
                     VPAKPGAKQENPLFSLEWANTNNTNNNTAKLIPIAESTDIKYPGFAQARPALIFQYGI
                     DDLDASAETVVVSSCSKIAKPGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYA
                     VGRIAGEEGLFRFQLDDKGKWGNPQLLAKKIRHMKVRYIYVSDCPEDDDAGKEETFKY
                     TGTFDSSTNAVTPAGVEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2320..2913
                     /locus_tag="WIB30_001926"
     CDS             2320..2913
                     /locus_tag="WIB30_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ3797935.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKITFSENCAKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCTANSTNLCIDNKGTEYKKGTQSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2918..3391
                     /locus_tag="WIB30_001927"
     CDS             2918..3391
                     /locus_tag="WIB30_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ3797936.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSSDVGCGAFSNRKK"
BASE COUNT          996 a          693 c          853 g          900 t
ORIGIN      
        1 aatttaatcc actatacatt accagttaac gtcctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcatgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacggcca acaataaatg tgactccggc
      301 aagaaggagc agggaatgtt ggctttcggc gacaaaaacg gcaataagga atataatgat
      361 acggcggatg ttcttctccg cagtgtggta ttgaatgatg atatcaataa taagcggatt
      421 aattatgcct tcaaccatat cgctttcggt cacacgcaac cgaccgccga ccgcgttgtt
      481 tggacgttca atcaaaacgg gacgttcggt tatacgaaag accggcatct tacaaaacaa
      541 tccagctttt tttactccga cggttatatc caaatcgtgc tgacagatgc gagagcagtt
      601 tcagatgccg ataagaaatt ccgttcggcg gtggttttga ttgacagcag cggcagggtt
      661 gaggtttgtc ctagaaacga taggcgtacc gtatgccaat ataaatgatt gttttagtcg
      721 gtttgggaaa taaaaaaatg aagaataatg attgcttccg cctgaaaaac ccccagtccg
      781 gtatggcgct gatagaagtc ttggtcgcta tgctcgttct gaccatcggt attttggcac
      841 tattgtctgt tcagttgcgg acagtcgctt ccgtcaggga ggcagagacg caaaccatcg
      901 tcagccaaat cacgcaaaac ctgatggagg gaatgttgat gaatccgacc attgattcgg
      961 acagcaacaa gaaaaactat aatctttaca tgggaaacca tacactatca gctgtgaatg
     1021 gtgagtttgc gattgatgcc ataaaaacta agacgcagtt ggcagaggaa caattgaaga
     1081 gatttagtta tgagctgaaa aatgccttgc cggatgcggc agtcatccat tacgccgtct
     1141 gcaaggattc gtcgggtaac gcgccgacat tgtccggcaa tgctttttct tcaaattgcg
     1201 acaataaggc aaacggggat actttaatta aagtattgtg ggtaaatgat tcggcagggg
     1261 attcggatat ttcccgtacg aatcttgagg tgagcggcga caatatcgta tatacttatc
     1321 aggcaagggt cggaggtcgg gaatgagacg taaaatgcta aacgtaccaa aaggcagtta
     1381 tgatggtatg aagggtttta ccattattga atttttggtt gcgggcatgc tcagtatgat
     1441 tgtcctgatg gcggtcggat cgagttactt cacgtcccgg aaattaaatg atgcggcaaa
     1501 cgagcgtctt gccgcacaac aggatttgcg gaatgcggca acattgattg tccgcgatgc
     1561 aagaatggca gggggcttcg gttgtttcaa tatgtccgag catactaaaa acgatattat
     1621 tgttgatcca agtaagcaaa ctcaacctgt ccctgcaaaa cccggtgcca aacaagaaaa
     1681 tccccttttt tctttagagt gggctaatac taataatact aataataata cagctaaatt
     1741 gattcctatt gctgaatcca cagatattaa atatccgggt tttgcccagg ctcgtccggc
     1801 attgattttc caatacggaa tcgatgatct tgatgcgagt gctgagactg ttgtagtcag
     1861 cagctgttcc aaaatagcaa aaccgggtaa gaaaatatct accttgcaag aagcaaagag
     1921 tgcattacag attactaatg atgataaaca aaatggaaat atcacccgtc aaaggcatgt
     1981 ggtcaatgcc tatgcggtcg gcaggattgc cggtgaggaa ggtttgttcc gcttccaatt
     2041 ggatgataag ggcaagtggg gtaatcctca gttgctcgcg aaaaagatta gacatatgaa
     2101 agtgcggtat atctatgttt ccgactgtcc tgaagatgac gatgccggca aagaggaaac
     2161 attcaaatat acgggtacat tcgacagctc cacaaatgct gttacgcccg ccggggtgga
     2221 ggttttattg agtagcggta ctgataccaa gattgccgct tcttcagaca atcatattta
     2281 tgcttaccgt atcgatgcga caatacgcgg gggaaatgta tgcgcaaaca gaacactttg
     2341 acgggaatcc cgacttctga cggacagagg gggtttgcac tgtttatcgt gctgatggtg
     2401 atgatcgtcg tggctttttt ggttgtaact gccgcgcagt cttacaatac cgagcagcgg
     2461 atcagtgcca acgaatcaga caggaaattg gctttgtctt tggccgaggc ggctttgcgg
     2521 gaaggcgaac ttcaggtttt ggatttggaa tatgatacgg acagtaagat tacatttagc
     2581 gaaaactgtg caaaaggtct gtgtaccgca gtgaatgtgc ggacaaataa tgataatgaa
     2641 gaggcttttg acaatatcgt ggtgcaaggc aagcccaccg ttgaggcggt gaagcgttct
     2701 tgcactgcaa attctaccaa cctgtgcatt gacaataaag ggacggaata taagaaaggc
     2761 acgcaaagcg tcagcaaaat gccacgttat attatcgaat atttgggcgt gaagaacgga
     2821 gaaaatgttt atcgggttac tgccaaggct tggggtaaga atgccaatac cgtggtcgtc
     2881 cttcaatctt atgtaagcaa taatgatgag taataaaatg gaacaaaaag ggtttacatt
     2941 gattgagatg atgatagtcg tcgcgatact cggcattatc agcgtcattg ccataccttc
     3001 ttatcaaagt tatattgaaa aaggctatca gtcccagctt tatacggaga tggtcggtat
     3061 caacaatatt tccaaacagt ttattttgaa aaatcccctg gacgataatc agaccatcaa
     3121 gagcaaactg gaaatatttg tctcaggcta taagatgaat ccgaaaattg ccgaaaaata
     3181 taatgtttcg gtgcattttg tcaataagga aaaaccaagg gcatacagct tggtcggcgt
     3241 tccaaagacg gggacgggtt atactttgtc ggtatggatg aacagcgtgg gcgacggata
     3301 caaatgccgt gatgccgctt ctgcccgagc ccatttggag accttgtcct cagatgtcgg
     3361 ctgtggagcc ttctctaatc gtaaaaaata gggctgtttt gccgatgccg tctgaaaatc
     3421 aatgttcaga cggcattttt at
//
LOCUS       DAWHRA010000165         3302 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWHRA010000165 DAWHRA010000000
VERSION     DAWHRA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.165"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            86..1100
                     /locus_tag="WIB30_001928"
                     /pseudo
     CDS             86..1100
                     /locus_tag="WIB30_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1316..2653
                     /gene="ribB"
                     /locus_tag="WIB30_001929"
     CDS             1316..2653
                     /gene="ribB"
                     /locus_tag="WIB30_001929"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ3797937.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIT
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADEETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2813..2950)
                     /locus_tag="WIB30_001930"
     CDS             complement(2813..2950)
                     /locus_tag="WIB30_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ3797938.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKEKIQKTKATGIYR
                     K"
     gene            complement(2961..3287)
                     /locus_tag="WIB30_001931"
     CDS             complement(2961..3287)
                     /locus_tag="WIB30_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ3797939.1"
                     /translation="MGEKIKDGKNQKTVLSREEEQKICNTFTHKQAVEDFSVVIGYDE
                     IKAKNHSLSAWQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKDRLA
                     MLKFNS"
BASE COUNT          865 a          906 c          739 g          792 t
ORIGIN      
        1 aagttacagg gtgcggatgc aaacgcattg cgagcgcggg tttgaggcat acgcgcaaac
       61 atcttaatat aatggattga tatttatgat tttctccatc atcgtcccta tttacaatgt
      121 ggaaaatacc tccgctgctg cgtggattcc gtgcttgccg aaaattttgc cgattatgaa
      181 atgattttgg tcgatgacgg ttcgccggac ggctgcggga agatttgcga cgaatatgca
      241 ggcaaatatc cgcatataaa agtgattcat caagaaaacg gcgggctgtc ggatgcccgc
      301 aacgccggta tccgggcggc aaaaggcgat tacctaatct ttttggacag cgacgactat
      361 tgggccgaca ccaatcgttc aaaaaacgcg cggggggggg gattctcttt gatttacaac
      421 aacttgcaga caaaaaggtt gatttgatcc tgcatccctc gtccttcaat taccgcgaca
      481 tccccaaagg ggcggacttt tcggataatg attttgtccg ccattttgaa acgctggtgg
      541 aggggcggta ctatatcgcc aacgcgtgga caaaaatcgt caggcgggaa ataatcatca
      601 aaaacaatct gtttttccca aaaggataca ttcacgagga tttcccgtac agtttgcaat
      661 tggcgcgttt tatcaagact tttgcctttt acgataaccc tttttaccag taccgcgttc
      721 tcggcggctc tatcagccac aacatcaaat acaaaaattt cagcgatgtg ctgacgcatc
      781 tcgaccgggg tgtggatttt ttagtcgaaa acaaaaattc ccccatctac ggcggtttgc
      841 aaaaatttgt cttcgacaat atcggctatc tgagatctat atcggtaagg ctttattttt
      901 ccaaaaacat tatcgttgtc taccgagaat atttttcatt taaagagaaa tgcagaaaga
      961 tattcggcgc gaaggcaatc cgtccgattt ttatcgggaa gaccgcattc atcataggat
     1021 tgccgatatt gcgcctgctc gtaccgccta tgctgtaccc ggcaatcaag gccgtttatc
     1081 agaaattttt ttcggaataa gcttccggca aaccccgaat cggaagcggg cgggaagaaa
     1141 cagccgcccc gccggcgggg attgcggcaa tgccgtctga agccacgaat ccggcttcag
     1201 acggcatctg tttaccaaaa agcaaataat tcggtttggc gaaaaaaaca gatttgcttt
     1261 ttggtaaata cgcgattaca atccgctaca tccgatttct acaaaggatg aaacgatgac
     1321 cgacacagct ggtctgcgcc gccgcaacct gcggcaatgg atagcagaac actacggcgg
     1381 tttgcaaacc cgttttgccg aagccgttgc cctcaacaca ggcgaactct ccgccctttt
     1441 gaaaaacaaa tccttcggcg agaaaaaagc ccgtaaaatc gaacaggcgg caaaaatgcc
     1501 cgccttctgg ctcgataccg aacacaccgc ccgcccgccc gaacacacag gaaaacacac
     1561 catgtcccat atttccccca tccccgaaat cctagccgac atcaaagccg gcaaaatggt
     1621 catcatcacc gatgccgaag accgagaaaa cgaaggcgac ctgctgatgg cggcgcaatt
     1681 cgtcacgccc gaagccatca acttcatgat caaacacgcg cgcggcttgg tctgcctgcc
     1741 gatggacggc gaaatggtcg aaaaactcgg gctgccgatg atgacccaaa aaaacggcgc
     1801 gcaatacggc accaacttta ccgtctccat cgaagccgca cacggcatta ccaccggcat
     1861 ttccgccgcc gaccgcgccc tgactattca aaccgccgtt tccccgaccg ccaaacccga
     1921 agacatcgtc caacccggtc atatctttcc gcttcgcgcc caaaaaggcg gcgtactcgt
     1981 ccgcgccgga cacaccgaag ccggcgtcga cctggcgcaa atgaacgggc tgattcctgc
     2041 cgccgttatt tgcgaaatca tcaacgacga cggcacgatg gcgcgtatgc ccgaactgat
     2101 gaaattcgcc gaagaacaca agctcaaaat cggcaccatt accgacctca tcgaataccg
     2161 cagccgtacc gaaagcctgc ttgaagacat gggcaacgca cccgtacaaa ccccgtgggg
     2221 cgagttccaa caacacgttt acgtcgacaa gctctccggc gaaacccacc tcgccctcgt
     2281 caaaggtaca ccttccgccg acgaagaaac cctcgtccgc gtccacgaac ccttcagcgt
     2341 catggacttc atccaagcca acccgcgtca ttcatggtcg ctgcccaaag cactcgagcg
     2401 catccaacag gccgaaagcg gcgtagtcat ccttctgcac cgcaccgaag acggcgcatc
     2461 cctgctcgac cgcaccctac ccaaaggcgc aaaccaagcc tacaaatggg acagcaaaag
     2521 ctacggcatc ggcgcacaaa tcctcgtcgg cctcaacgtc aaaaaactgc gcgtcctcgg
     2581 gcagccctca tcttttaccg gcctgaccgg cttcggtttg gaagtcgtcg gctttgaaga
     2641 agcggaaaaa taatatagta aattcaaata ctttatattt tctttattta ttgcattatt
     2701 tccgtgcaaa cgaaaacccg gtctgttggg ttggattttg tttttgcaaa tttcgggtaa
     2761 cttctaattc gtcattcccg cgcaggcggg aatccggttc gtcgggtttt tgtcatttcc
     2821 gataaattcc tgtggctttg gttttttgga ttttctcttt cagggaaaga acggcataag
     2881 tattttccaa accaaacaaa atgccgtctg aaaggctttc agacggcatt ttaagtttga
     2941 ccggtttcat tcatcggtat ttatgaattg aatttcaaca tcgccaatct atccttaatc
     3001 tctttttcca attcggcaga ttcggcgaaa agtttatcca aatccgctga aaatcccgcc
     3061 attttttgcg caaattcgtc ggcggaaata tccacataat caatctttac ctcgaaatac
     3121 tgccacgccg acaagctgtg attcttcgct ttgatttcat cgtagccgat caccacgctg
     3181 aaatcttcca ctgcttgttt gtgcgtgaaa gtattgcaga ttttttgttc ttcttcgcga
     3241 gaaagtacgg ttttttggtt tttgccgtct ttgatttttt cgcccaagcc cgatgcgtcg
     3301 at
//
LOCUS       DAWHRA010000166         3268 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWHRA010000166 DAWHRA010000000
VERSION     DAWHRA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.166"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(100..1731)
                     /locus_tag="WIB30_001932"
     CDS             complement(100..1731)
                     /locus_tag="WIB30_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ3797940.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAKLSTRAVPYVGTALLAHDVYETFKEDIQARGYQYDTETDKFV
                     KGYEYSNCLWYEDERRINRTYGCYGVDSSIMRLMSDYSRFPEVKELMESQMYRLARPF
                     WNWHKEELNKLSSLDWNNFVLNSCTFDWNGGDCSVNKGDDFRNGANFSLIRNPKYKEE
                     MDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVV
                     ATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPAPNENPGTR
                     PNPEPDPDLNPDANPDTDGQPGTRPDSPAVPGRTNGRDGKDGKDGKDGGLLCKFFPDI
                     LACDRLPESNPAEDLNLPSETVNVEFQKSGIFQDSAQCPAPVTFTVTVLDSSRQFAFS
                     FENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            complement(2078..2380)
                     /locus_tag="WIB30_001933"
     CDS             complement(2078..2380)
                     /locus_tag="WIB30_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797941.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGTD"
     gene            complement(2408..2638)
                     /locus_tag="WIB30_001934"
     CDS             complement(2408..2638)
                     /locus_tag="WIB30_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797942.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDASAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            complement(2876..3076)
                     /locus_tag="WIB30_001935"
     CDS             complement(2876..3076)
                     /locus_tag="WIB30_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797943.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEMGGGFQIFQFFVKSE"
BASE COUNT          918 a          774 c          630 g          946 t
ORIGIN      
        1 aaagatttat aaagacgata aagccgtctt tacaaatctt tctggacgtc ctccccctgc
       61 cttggtacaa gttactgaag cccggcggtg ctgcgcctgc tagacttcac gagatactgt
      121 gcggatacaa aaaaaggcgg caaccgccca agcaagggcg agaagcatgt accttagccg
      181 ttcggctatg gtacatgcgt tctcaaagct gaacgcgaac tgcctgcttg aatcaagcac
      241 agtcactgtg aaagtgacag gtgcgggaca ctgtgcggaa tcttgaaaga ttcctgattt
      301 ctgaaactct acattgacgg tttcagacgg cagatttaaa tcttctgccg gattggactc
      361 gggcagcctg tcgcaagcga gaatgtcggg gaagaatttg cacaaaaggc cgccatcttt
      421 gccgtccttt ccgtctttgc cgtccctgcc gtttgtgcgt cccggaacgg cgggggaatc
      481 gggtcttgtg ccgggctgtc cgtccgtatc gggatttgca tcgggattca aatcggggtc
      541 gggttcggga ttgggacgcg tgccggggtt ctcattgggg gccgggttgt ttgcggggtt
      601 ttcagcgggc gatacttcgg gcagcggctg tgcgttcggc gcttccgcgc ttccgggggt
      661 taagtcggga cgcggaatta cttgaacatc cactgtggtg ttgccttgcg aatccctgcc
      721 gaatgttgcg acaacctgaa cgggattccc gttcctgtcc gtgacgggac ccatattcac
      781 ttttgttccg ggtgcgactt ctactttttc ggaataaccg ggataaccgg ttgcctttat
      841 gtatttgtcg ggattggcat cgactttcaa cgataaaatc tcttccagct ttttggcatc
      901 catttcttct ttgtatttcg gattgcggat aagggaaaaa ttagccccat ttctgaaatc
      961 atcaccttta ttgaccgaac aatctccgcc gttccaatca aatgtgcaac tatttaaaac
     1021 aaaattattc caatccaaag aacttaattt attcagttct tctttatgcc aattccaaaa
     1081 cggacgtgcc agcctataca tttggctttc catcaattct ttaacttcgg ggaatctgct
     1141 gtaatcggac ataaggcgca taatcgaact gtcaacgccg tagcagccat aggttctatt
     1201 aatacgtctt tcgtcttcgt accaaaggca attactatat tcgtagcctt ttacaaattt
     1261 gtcggtttcg gtgtcgtatt ggtagcctcg tgcctgtatg tcttctttga aagtttcgta
     1321 tacgtcatgg gctaaaaggg ctgttccgac atagggaacc gcccttgtgc ttaatttcgc
     1381 gcctaagcgg gcaagtttgc cgacccccgc caatacgcca gcgcgggaaa ctgatgaaga
     1441 tacaaccgaa ctaactttac ccgtacttga tgctccagta gatacatgcc taacttttaa
     1501 atcttgagct tctgaagtta aatatcttcc cgattctcta tcaaatacac ctttccaaat
     1561 tcttcttctt ttgtcatctg aacctcgaat acctaaatat ctgccatcat cataaacttc
     1621 aaccctatca aatttacccg gagcatttac tgatgccaat gcaaatttag atacaaaaaa
     1681 actaaagcat aggataatct ttaaaaaatt tcgccctagg ataataaaca taacattttg
     1741 cttcatctaa tttaattaat aatgtagaag aatcagggaa ttgaatttct ataaaatctc
     1801 tatcataacg atgaaagaaa ttcctaaaaa tatcaaatga aaacgaagtt ttaaaaaccc
     1861 cttttctaat caattcccta taataaatta acgcttgtgg taaatctcta taaatatgtg
     1921 aatcagtttg aaatttaact aaatgatatt cagaaataaa cataattcgg accttttaaa
     1981 acactctaaa aagccgattc taccaaccgt taccaaaaca gccaagcaac agacaaagcc
     2041 aagaagaaac cgagaagaaa ccagaaatct ataatcatca atcagtccca accttgccta
     2101 tgtcttttaa aaaattaatc agaagcctga aaccgtaaat gactacaaac agaattagaa
     2161 ccgtcgaacc gacataagaa ccttgcttta tctgctcaaa attggaacat tccggataag
     2221 acaacgtaac cggctttccg ttcaaaatcc atttatcgcc caccctttcc ggcctgatga
     2281 tttttccgtc ctgggtaaca gtaggaggaa gggacgacaa taaatagtcg tctgcctgca
     2341 atcttgtatc aaaacaattt atgccgacac gatagcccat ttatacgccc ctttttcttc
     2401 actctgttta tttgacagat ttgatcatgc tccaagccat cttgaagcct tggattgcca
     2461 agatgaccgt aatggccgcc atacccacgg cggaaaccat tgccacaaaa cccatgatta
     2521 cattcgccac ttgcgtacca atcgcggatg catcaaagcc atctgccata acaatagccg
     2581 gtgtgaagat accagctgcc aacaatgctt ttacagcata ttttttaacg atgttcatcg
     2641 tttttttcct ttttttgata tttaaaataa gacgacttct tgacttgctt catccggacg
     2701 aagtcttttc cgaatctcgt ttttagccga taaaatagag gattgcgaaa agaaaaagaa
     2761 acatacagac cacccagccg ataattagtg ttgcaaggtt cattttcatg atatttttcc
     2821 tttgttgcgg gctttgtgaa aggttaacag accgcccgcc gagcctgttt ttcttttatt
     2881 ccgattttac gaagaactga aatatctgga atcctccgcc catttcattt atgcctgaat
     2941 tcaacgcatc ttcgtaactt tcaaattgac ctgctgattt aatattttga gtaaacccca
     3001 catcaccgaa cggatcggga taaataaagt catgcgtttc taaatcttga actatgaaac
     3061 gttcttcaaa tttcataaat caacctttcg gcttttccgc cacctgaaaa tcaattaatg
     3121 acggaaccat gcctttacct gtcgaagtca tttcaaccat taccataact tcgcacggat
     3181 atttgagatt ctctaacctt gagaaattct tactgtcccc gaacttcatt tgtgctgccg
     3241 taaatccaac agcattaccc gactgtgc
//
LOCUS       DAWHRA010000167         3240 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWHRA010000167 DAWHRA010000000
VERSION     DAWHRA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.167"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..688
                     /locus_tag="WIB30_001936"
     CDS             <1..688
                     /locus_tag="WIB30_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ3797944.1"
                     /translation="IEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHEHGKIWK
                     KPTRHEDIAAFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSKLGRL
                     AYYPEALVKYRLHANQVSSKYSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQI
                     KAVAYELLEKHLPEEDFERARRFLYQCFKRTDTPPAGAWLDFAADGRMRRLFTLRQYF
                     GILRRLLKNR"
     gene            730..1569
                     /locus_tag="WIB30_001937"
     CDS             730..1569
                     /locus_tag="WIB30_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ3797945.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHTVLWKQALDESLPYIAVFEDDVLLGEGAEKFLA
                     EDGWLKERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPSEGLHPVDWMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQLIGKIIV
                     PFQ"
     gene            1581..2500
                     /locus_tag="WIB30_001938"
                     /pseudo
     CDS             1581..2500
                     /locus_tag="WIB30_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
     gene            2559..3240
                     /locus_tag="WIB30_001939"
                     /pseudo
     CDS             2559..3240
                     /locus_tag="WIB30_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          830 a          832 c          884 g          694 t
ORIGIN      
        1 gattgagaaa atcgtgggtg agatggaaaa agaccgcagc atcatcgcaa tgggcgcgtg
       61 gctggaagtt ttgtcggaag aaaaggacgg caaccggctg gcgcggcacc acgaacacgg
      121 caaaatttgg aaaaagccga cccggcacga agacatcgcc gcctttttcc cttttggcaa
      181 ccccatacac aacaacacga tgattatgag gcgcagcgtc attgacggcg gtttgcgtta
      241 caacaccgag cgggattggg cggaagatta ccaattttgg tacgatgtca gcaaattggg
      301 caggctggct tattatcccg aagccttggt caaataccgc cttcacgcca atcaggtttc
      361 atccaaatac agcgtccgcc aacacgaaat cgcgcaaggc atccaaaaaa ccgccagaaa
      421 cgattttttg cagtctatgg gttttaaaac ccggttcgac agccttgaat accgccaaat
      481 aaaagcagta gcgtatgaat tgctggagaa acatttgccg gaagaagatt ttgaacgcgc
      541 ccgccggttt ttgtatcaat gtttcaaacg gacggacacg ccgcccgccg gcgcgtggct
      601 ggattttgcg gcagacggca ggatgcggcg gctgtttacc ttgaggcaat acttcggcat
      661 tttgcgccga ttgctgaaaa accgttgaaa aacgccgctt tatccaacag acaaaaaaca
      721 ggataaatta tgcaaaacca cgttatcagc ctggcttccg ccgcagaacg cagggcgcac
      781 attgccgata ccttcggcag gcacggcatc ccgtttcagt ttttcgacgc actgatgccg
      841 tctgaaaggc tggagcaggc aatggcggaa ctcgtccccg gtttgtcggc gcacccctat
      901 ttgagcggag tggaaaaagc ctgctttatg agccacaccg tattgtggaa gcaggcgctg
      961 gacgaaagct taccgtatat tgccgtattt gaggacgacg ttttactcgg cgaaggcgcg
     1021 gagaaattcc ttgccgaaga cggctggcta aaagagcggt ttgacccgga ttccgccttt
     1081 atcgtccgtt tggaaacgat gtttatgcac gtcctgacct cgccctccgg cgtggcggac
     1141 tactgcgggc gtgccttccc gcttttggaa agcgaacact gggggacggc gggctatatc
     1201 atttcccgaa aagcgatgcg gtttttcctg gacaggtttg ccgccctgcc gtctgaaggg
     1261 ctgcaccccg tcgattggat gatgttcggc aaccctgacg acagggaagg aatgccggtt
     1321 tgccagctca atcccgcctt gtgcgcccaa gagctgcatt atgccaagtt tcacgaccaa
     1381 aacagcgcat tgggcagcct gatcgaacac gaccgcctcc tgaaccgcaa acagcaaagg
     1441 cgcgattccc ccgccaacac attcaaacac cgcctgatcc gcgccttgac caaaatcagc
     1501 agggaaaggg aaaaacgccg gcaaaggcgc gaacagttaa tcggcaaaat cattgtgcct
     1561 ttccaataaa aggagaaaag atggacatcg tatttgcggc agacgacaac tatgccgcct
     1621 acctttgcgt tgcggcaaaa agcgtggaag cggcccatcc cgatacggaa atcaggtttt
     1681 acgtcctcga tgccggcatc agtgaggcaa accgggcggc ggttgccgcc aatttgcggg
     1741 ggggggtaat atccgcttta tagacgtaaa ccccgaagat ttcgccggct tccccttaaa
     1801 catcaggcac atttccatca cgacttatgc ccgcttgaaa ttgggcgaat acattgccga
     1861 ttgcgataaa gtcctgtatt tggatataga cgtattggtc agggacagcc tgaagccctt
     1921 atgggatacc gatttgggca ataactgggt cggcgcgtgc atcgatttgt ttgtcgaaag
     1981 gcaggaaggc tacaaacaaa aaatcggtat ggcggacggc gaatattatt tcaatgccgg
     2041 cgtattgctg atcaacctga aaaagtggcg gcagcacgat attttcaaaa tgtcctgcga
     2101 atgggtggaa caatacaagg acgtgatgca atatcaggat caggatattt tgaacgggct
     2161 gtttaaaggc ggggtgtgtt atgcgaacag ccgtttcaac tttatgccga ccaattatgc
     2221 ctttatggcg aacaggtttg cgtcccgcca taccgacccg ctttaccgcg accggaacaa
     2281 tacggcgatg cccgtcgccg tcagccatta ttgcggcccg gcaaagccgt ggcacaggga
     2341 ctgtaccgct tggggtgcgg aacgtttcac agagttggcg ggcagcctga caaccgttcc
     2401 cgaagaatgg cgcggcaaac ttgccgtccc gcccgcgaag cgtatgtttc aaagatggcg
     2461 cagaaagctg tctgccagat tcttacgcaa gatttattga cggggcacgc cgtctgaagc
     2521 cttcagacgg catcggacgt atcggaaagg ggaacggatt gcaaccttta gtcagcgtat
     2581 tgatttgcgc ctacaacgta gaaaaatatt tcgcccaatc attagccgcc gtcgtgaatc
     2641 aaacttggtg caacttggat attttgattg tcgatgacgg ctcgacagac ggtacgcttg
     2701 ccattgccaa ggattttcaa aagcgggaca gccgtatcaa aatccttgca caagctcaaa
     2761 attccggcct gattccctct ttaaacatcg ggctggacga attggcaaag tcgggggggg
     2821 gaatatattg cacgcaccga tgccgacgat attgccgccc ccgactggat tgagaaaatc
     2881 gtgggcgaga tggaaaaaga ccgcagcatc atcgcgatgg gcgcgtggct ggaagtgttg
     2941 tcggaagaaa aggacggcaa ccggctggcg cggcatcaca ggcacggcaa aatttggaaa
     3001 aagccgaccc ggcacgaaga tattgccgac tttttccctt tcggcaaccc catacacaac
     3061 aacacgatga ttatgcggcg cagcgtcatt gacggcggtt tgcgttacaa caccgagcgg
     3121 gattgggcgg aagattacca attttggtac gatgtcagta aattgggcag gctggcttat
     3181 tatcccgaag ccttggtcaa ataccgcctt cacgccaatc aggtttcatc caaatacagc
//
LOCUS       DAWHRA010000168         3234 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWHRA010000168 DAWHRA010000000
VERSION     DAWHRA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.168"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(62..442)
                     /locus_tag="WIB30_001940"
     CDS             complement(62..442)
                     /locus_tag="WIB30_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ3797946.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            919..3000
                     /locus_tag="WIB30_001941"
     CDS             919..3000
                     /locus_tag="WIB30_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ3797947.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPDRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVVLWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2990..3184
                     /locus_tag="WIB30_001942"
     CDS             2990..3184
                     /locus_tag="WIB30_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ3797948.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
BASE COUNT          682 a          955 c          832 g          765 t
ORIGIN      
        1 tccgtccgac acaaaaaacc gcccgaaaaa tctggacggc ggttcaaaca ggctgccccg
       61 tttaacgggc gcggcaggaa gtttcgaccg aattgccgta ggcatcggta aagccgaaaa
      121 aggcttcgcc gcctttctgg tgccactcgg ttgcgtttcc gaacaaaccg tgttcggcgg
      181 tatagcgttc gccggatgcg gcaacgtcgg aagagaggac ggcacgcctg ccgtccagcc
      241 gcaacgcgac tttgccgctg tccaaatggc ggacgcgcac agacaaaccg ttctcgcagg
      301 aaaacgcccg aaaatcgtcc gtgccggctt ggttttgaac gggcggcata tgcccgcgtc
      361 cgccgtcatc atacgcctcc ggcacggcac aggccgccaa agacaaaacc ggtacggtca
      421 gcgcgaaaaa cctgatattc ataaaagctc cccaataaaa ataagatatg aaacaaccgc
      481 cctgattcca agctgcggca acgccatact ataaacggac gcgcaaacac acaagcccga
      541 taaccggaat ttacctgcga tgaatcaata atccggattg cgcgcccctt ctttacccct
      601 cttccgatgc cgccttttgc ctgacgatgc cgtctgaacc tgcctgcccg cccggaggaa
      661 tgtaaatttt ttccaaattc caagtaaaaa ccgctatcgg tgtgctaatt tgcgttaaaa
      721 tcctattcgg cgtttaacgt tttgtgcgcc cgcatccctg cactgtttga tgcgggcata
      781 aggcacaaat cccgacaagc gcactgtttc atacttcgtc aatcattcag actccggttt
      841 gtgcccgtgc cggcagatgg ttcggccgtt tcccgccgtt caggcatatt ccgacagtgt
      901 gagataagga tttattcgat gaaatcactc aaaaccttcc tcatttgggg catagtggta
      961 ctggtcggct tagcatcctt taccactctg gccctcagcc gaggcgaaca ggtcagcgcg
     1021 gtatggatgg tcaccgccgc catatccgtt tactgcatcg cctaccgttt ttacagcctc
     1081 tacatcgcca accgcgtaat gcggctcgat cctgaccgcc tgactccggc agaacgccac
     1141 aacgacggct tggactacgt tccgacgcac aaaggcgtat tgttcggaca ccactttgcc
     1201 gcaattgccg gcgcgggccc tttggttggt ccggttttgg cggcgcaaat gggttatctg
     1261 cccggtactt tgtggattat cttcggcgtg gtatttgccg gcgcggtaca ggatatgatg
     1321 gtcttgttcg tctctatgcg ccgcgacggt aagtctttgg gcgatattgt gaaacaggaa
     1381 ctcggcactg tccccggcgt gattgcctcc atcggtattt tgatgattat ggtcatcatt
     1441 atggcggtgt tggcgttgat tgtcgtaaaa gcattggttc acagcccttg gggtacgttc
     1501 accattgccg caacgatgcc gattgcgctg tttatgggta tctacacgcg ttatatccgt
     1561 ccgggcaaaa ttggcgagat ttccatcgtc ggctttattt tgctgatgtt ggccgtgatt
     1621 tacggcgaag atgtggcgaa aagttccatc ggacattggt tcgaccttga cggcatccag
     1681 ctcacttggg cgattatgat ttacggcttt gtcgcctccg tattgcccgt atggttgctg
     1741 ctcactccgc gcgactatct ctccaccttc ctgaaaatcg gtacgattgc ggccttggct
     1801 ttgggcatcg tcatcgtcaa tcccactttg caaatgcctg ccgtaaccca ctttatcgac
     1861 ggttcgggtc cggtattctc aggcgcattg ttcccgttcc tcttcatcac catcgcctgc
     1921 ggtgcggttt cgggcttcca cgcgctgatt tcctccggca ccacgccgaa aatgctggaa
     1981 aacgaaaccc acgtccgtat gattggttac ggcggtatgt tgatggaaag tttcgtagcc
     2041 attatggcgc ttgccgccgc cgcatcgctt gatcccggcg tgtacttcgc catgaacagc
     2101 cctgccgccc tgatcggtac ggatgccaat accgccgccg aagtgattac caccaagctg
     2161 caattccctg tcgatgcggc aaccctgttg cacaccgcta aggaagtggg cgaaaacacc
     2221 atcctttccc gtgcaggcgg tgcgcccacc ctcgcagtcg gtatggcgca cattatgagc
     2281 cgcctgattc cgggcgaggc gatgatggcg ttctggtatc acttcgccct gttgtttgaa
     2341 gccttgttca tcctgaccgc cgtcgatgcc ggtacgcgcg tcgcacgttt tatgattcaa
     2401 gacttgggca gcatcttcta caaacctttc ggcaacaccg actccatccc cgccaacctg
     2461 attgcgacct tcttcgccgt ggtattgtgg ggctacttcc tctacaccgg cgtaaccgac
     2521 ccgctgggcg gcatcaactc gctctggcct ttgttcggca tcgccaacca aatgctggca
     2581 ggcgtggcct tgattatgtg cgccgtggtg ctgattaaga tgaaacgcga ccgttatgtc
     2641 tgggtggtac tcgttcccgc cgtcggcgta ctgttcgtaa cctgctacgc cggcctgcaa
     2701 aaactgttcc acagcgaccc gcgcatcagc ttccttgccc acgccggcaa atacagcgac
     2761 gcattggcta aaaacgaagt ccttgcgcct gccaaagaca tcggcgaaat ggcgcaaatc
     2821 atcttcaacg acaagattaa tgccggtctg accatcctct tcttgtcggt tgtcgtgatt
     2881 gtcgccgcgt acggtttgcg taccgccctc aaagcacgca aagtcggctg gccgaccgcc
     2941 aaagaaatcc cggcggtgta ccgcgacggc aaacagccgg aggcacaaag tgaagcataa
     3001 gctcgcgtct tggtggaaaa ccatcaagct gacggcaaac ttgatggcag gcgtgcccga
     3061 ttatgaaaac tacgttgcac agcagcgcaa acataatccc aacgcccctg tgatgaccaa
     3121 gctgcagttt caagactatt gccgcaaacg ccgctgcggc gcaaacggcg gacgctgctg
     3181 ttaagcctgc ttgaaacaaa ttccgtctga acgccgcttc agacggaatt ttta
//
LOCUS       DAWHRA010000169         3210 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWHRA010000169 DAWHRA010000000
VERSION     DAWHRA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.169"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..593)
                     /locus_tag="WIB30_001943"
     CDS             complement(<1..593)
                     /locus_tag="WIB30_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ3797949.1"
                     /translation="MDRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPPADFLELLSLCRDIHALTEGAFDPTVQVLWKLYADYFAAHPDAETPPPQN
                     SVKEALKRVGFGKVSFDDREIRFAEKGMGLSLNGIAQV"
     gene            complement(604..1398)
                     /locus_tag="WIB30_001944"
     CDS             complement(604..1398)
                     /locus_tag="WIB30_001944"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ3797950.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQTAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1782..3116
                     /gene="gdhA"
                     /locus_tag="WIB30_001945"
     CDS             1782..3116
                     /gene="gdhA"
                     /locus_tag="WIB30_001945"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ3797951.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
BASE COUNT          838 a          911 c          771 g          690 t
ORIGIN      
        1 acttgcgcga tgccgttcaa ggacaaaccc ataccctttt cggcaaaacg gatttcacgg
       61 tcgtcaaacg agactttgcc gaagccgacc cgtttcaggg cttccttcac gctgttttga
      121 ggcggcggcg tttccgcatc gggatgagcg gcaaaataat cggcatacag tttccacaaa
      181 acctgaaccg tcggatcgaa tgccccttcc gtcagcgcgt gaatatcgcg gcacaggctc
      241 aacagttcca aaaaatccgc cggcggcgaa gtcagataac cgtcacggtt cagacggctg
      301 atcaggctgt cttcacggta aaggctgaac attttttcca aacgcgccac ttccgccaaa
      361 accttgttga ccaaatccgc cgcacgcctg tcgtccacac cgaacagacg gagctccgca
      421 ccggaaccca gtgcgacacc tttccagaaa aacacatttt cattgcgttt ttcatccccg
      481 ttgcgttttt catcatcggc ggcaaaagga ttcggcagga aagaaaccgc cgcgcccgcc
      541 gccgcaacgg cggcaaccgt cagaaaacgc ctgcgcccga aatgcctgtc catatacccc
      601 tcctcagacc gacactgccg ccttgatgtg cggatggggg tcgtagcctt ccaactcgaa
      661 atcttcaaac ttgaaggaaa acaaatcttt gacttcagga ttgattttca tcactggcaa
      721 ggcgcgcggt tcgcgttcca actgcaatgc ggtctgctcg aaatggttgc ggtacaaatg
      781 cgcgtcgcca aacgtatgga caaactcgcc cgcctccagc ccgcacactt gcgccatcat
      841 catggtcaac aatgcgtagc tggcaatatt aaacggcaca ccgaggaaaa tatccgcgct
      901 gcgctggtaa agctggcagg acagtttgcc gtcggcaacg taaaactgaa acagcgcgtg
      961 gcagggcggc aaggccattt catcgaccaa agccggattc cacgccgata caatcaggcg
     1021 gcgcgagtcg gggtttttct taatttgttc caccacattg gcgatttggt cgatatgcct
     1081 gccgtcgggc gcgggccagt tacgccactg gtagccgtaa accgggccta agtcgccgtt
     1141 ttcgtccgcc cactcgtccc aaatggaaac attgttgtcc tttaggtatt tgatattggt
     1201 atcgcctttg agaaaccaaa gcagctcgtg gataatcgag cgcaaatgca gctttttggt
     1261 cgtcagcagc ggaaaacctt tgcccaagtc aaaacgcatc tgataaccga atacggagcg
     1321 cgtgcccgta ccggtgcggt ctgatttgtc cgtaccgttg tcgaggacgt ggcgcatcaa
     1381 gtccaaatag gctttcatag cagtctttca tcaaattaaa cggcgcatat tgtaacattt
     1441 ccggataatg cccaaaacac ggatacaggc aggcaggatt gttggcaatt tcagtccttt
     1501 tccacagtaa aacccagtgg gaaaacaaaa ttaccttgat tggaatcaaa aaatctagtt
     1561 taattactta gaataaaatt tcaataatat ttttatttac gaaattaatt tatgattatt
     1621 gattaattat cggcaacaaa catcaaacat cgaaaacatg gaaaaaataa tgtcaacaat
     1681 ttttgccaaa tcgggcttgg catcagaaaa aaataggttt atattcccac ctacaaattt
     1741 gttttcccat tagtacacta tcaaccaaaa ggagtatccg aatgactgac ctgaacaccc
     1801 tttttgccaa cctcaaacaa cgcaatccca atcaggagcc gttccatcag gcggttgaag
     1861 aagtcttcat gagtctcgat ccgtttttgg caaaaaatcc gaaatacacc cagcaaagcc
     1921 tgctggaacg catcgtcgaa cccgaacgcg tcgtgatgtt ccgcgtaacc tggcaggacg
     1981 ataaagggca agtccaagtc aaccggggct accgcgtgca aatgagttcc gccatcggtc
     2041 cttacaaagg cggcctgcgc ttccatccga ccgtcgattt gggcgtattg aaattcctcg
     2101 cttttgaaca agtgttcaaa aacgccttga ccaccctgcc tatgggcggc ggcaaaggcg
     2161 gttccgactt cgaccccaaa ggaaaatccg atgccgaagt aatgcgcttc tgccaagcct
     2221 ttatgaccga actctaccgc cacatcggcg cggacaccga tgttccggcc ggcgacatcg
     2281 gcgtaggcgg gcgcgaaatc ggctacctgt tcggacaata caaaaaaatc cgcaacgagt
     2341 tttcttccgt cctgaccggc aaaggtttgg aatggggcgg cagcctcatc cgtcccgaag
     2401 cgaccggcta cggctgcgtc tatttcgccc aagcgatgct gcaaacccgc aacgatagtt
     2461 ttgaaggcaa acgcgtcctg atttccggct ccggcaacgt ggcgcaatac gccgccgaaa
     2521 aagccatcca actgggtgcg aaagtactga ccgtttccga ctccaacggc ttcgtcctct
     2581 tccccgacag cggtatgacc gaagcgcaac tcgccgcctt gatcgaattg aaagaagtcc
     2641 gccgcgaacg cgttgccacc tacgccaaag agcaaggtct gcaatacttt gaaaaccaaa
     2701 aaccgtgggg cgtcgccgcc gaaatcgccc tgccctgcgc gacccagaac gaattggacg
     2761 aagaagccgc caaaaccctg ttggcaaacg gctgctacgt cgttgccgaa ggtgcgaata
     2821 tgccgtcgac tttgggcgcg gtcgagcaat ttatcaaagc cggcatcctc tacgccccgg
     2881 gaaaagcctc caatgccggc ggcgtggcaa cttcaggttt ggaaatgagc caaaacgcca
     2941 tccgcctgtc ttggactcgt gaagaagtcg accaacgcct gttcggcatc atgcaaagca
     3001 tccacgaatc ctgtctgaaa tacggcaaag tcggcgacac agtaaactac gtcaatggtg
     3061 cgaacattgc cggtttcgtc aaagttgccg atgcgatgct ggcgcaaggc ttctaagcaa
     3121 acgccgccgc tccgcaaaca aaatgccgtc cgaaccgcaa atgctgttca gacggcattt
     3181 cattatccgc ccgttcgaat cgggtgatac
//
LOCUS       DAWHRA010000170         3185 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWHRA010000170 DAWHRA010000000
VERSION     DAWHRA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.170"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(75..668)
                     /gene="ribA"
                     /locus_tag="WIB30_001946"
     CDS             complement(75..668)
                     /gene="ribA"
                     /locus_tag="WIB30_001946"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ3797952.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(661..1335)
                     /locus_tag="WIB30_001947"
     CDS             complement(661..1335)
                     /locus_tag="WIB30_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797953.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSQQGEGLENIWNLDENVADVSLACRCAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1489..1573
                     /locus_tag="WIB30_001948"
     tRNA            1489..1573
                     /locus_tag="WIB30_001948"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1523..1525,aa:Leu,seq:tag)
     gene            2093..3127
                     /gene="purM"
                     /locus_tag="WIB30_001949"
     CDS             2093..3127
                     /gene="purM"
                     /locus_tag="WIB30_001949"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ3797954.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGVGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT          809 a          833 c          792 g          751 t
ORIGIN      
        1 ttattgcttt atcaaggtat ggaaacctgt ttcccgaaag gcggcgcagg atgcccgttc
       61 cctgcaactt tgccttattc cgacatcaga tgtcccagct tgtctgcttt ggtttggagg
      121 tagcgtttgt tttcaaggtt ttccccgacg tgcaggggga tgcgttcgac cacgttaatc
      181 cccgcatctt tcagggtttg gattttttcg gggttgttgg tcagcagttt gaccgagcgg
      241 atgcccagat attcgtagat agattgcgcc aaacggaaat cgcgggcatc gacgggcagc
      301 ccgagtgcca aattggcttc aaccgtatcc ataccttggt cttgcagatg ataggcgcgg
      361 attttgttaa tcagcccgat gccgcgtcct tcctgacgca gatagacgat gatgccgcgc
      421 ccctctgtct gtaccgccct catggccgct tcaagttgag gtccgcagtc gcattttctc
      481 gagaacagcg catcgcccgt caggcattcg gagtggatgc gcgtcagaac cggattgccg
      541 tctgaaaaat taccgacggt cagcgcgacg tgttcctgcc cgtttgcctc ttcaaagccg
      601 tgcatcgtaa atacgcccca ttcggtcggc aggcggcagg aagcaacata gtttaataat
      661 tcagacattt tcatctcctt ctgcggcagt gatgcccaaa agctgcttca aaggttcgga
      721 caaagccaag gcaagagcca cccattccac ctgtgctgcc gtacccgcac actctgcgca
      781 ttcaaattcc acatgaacca cgcccaacac accgccactt tccgtgcaga ccggaatgga
      841 aatttgcgcc gccgaagcat gattgcgttc tcccgaaagc tctcccaaat ccaaccaacg
      901 gcgtacatcc gaggcaacat tcatccagcc gctttgcgcg caacggcagg caagtgaaac
      961 atcggcaaca ttttcatcta gattccatat gttttccaaa ccttctccct gttgggacag
     1021 gcaaaccagc cgaaaggcgg cattttcaga cggcatcagg gcataaacgg ctgcggcgcg
     1081 cacgccttta cagcgcgaaa ataccgaatc cagagccatg aaaagccgtt tcagaccttc
     1141 ctcattcccg ctgttttgct ccaaataatc ggcaagcctc caatcctcat gctcaagcca
     1201 caaaaccgaa cggtctatcg aagccgtccc catatccatt accgtctgcg ccgtcagata
     1261 cgccgcccga acctcgtcaa gcggcagctt caaaccctga gtcagcagaa aatccttaat
     1321 caaagcagca ggcataccca aattccgtca ataaaaacaa aaaaccgccc gattccgatg
     1381 tcaggcggac gaaaaacaga ttttaccgcc ccaaacccca agccatcaac agccccgccg
     1441 aaaaccttgc acaaaaaaac aaaatctgta taatcgtcca attcccatgc ggacgtggcg
     1501 aaattggtag acgcaccaga tttaggttct ggcgccgaga ggtgtgagag ttcgagtctc
     1561 tccgtccgca ccaataatga taaataaatt atatagttaa tgcttatcgg gacaaattag
     1621 tgccacaaaa cataagtcgt ctgcttttca gcgggcggct tttttatctg cctatatgtt
     1681 tcaacacgca gaacggtaca taaagtgccg ccatttgttc cgcaccgaat cggaaacggg
     1741 tgcttccgca cgcaatcaga ttcaccaccc ctaatgccgg ggtttcaacc ggaaagaaga
     1801 aaatattgat tttgatgatg gcatcaaaaa accccttccg ctcgtctgcc aaaaggaatg
     1861 gttaagaaaa gcaatgcgtc ttttgatgga acgcgatata taaggaattg aggggaaggg
     1921 caggcgggtt tctgcccgtt aatcctgcgg acggtcccgc caaacccgac acgcgcgggc
     1981 atatggcgga ctgtttacaa tattgtctaa aaatctgaac ctgcaaacgt gaaataaggt
     2041 agaatacgcc ccgttatttt accgtccaat ccccatttac caaggaaaaa cgatgagtac
     2101 ttcattgagt taccgcgatg caggtgtcga tatcgacgca ggcgaccaac tggtcgaaaa
     2161 catcaaaccg tttgccaaac gcacgatgcg cccggaagta ttgggggatt tgggcggttt
     2221 tggcgcattg gtcgaaatcg gcaagaaata tcaaaatccc gtattggtca gcggtacgga
     2281 cggcgtgggt accaagctca agcttgcctt tgattgggat aaacatgata cggtcggcat
     2341 cgaccttgtt gcaatgagtg tcaacgatat tttggttcaa ggggctgagc ccttgttttt
     2401 cttggactat tttgcctgcg gtaaattgga tgttccgcgc gcgaccgatg ttattaaagg
     2461 cattgcccaa ggttgcgaag aatccggttg cgccctgatt ggcggggaaa ctgccgaaat
     2521 gccgggtatg tatcccgtcg gagaatacga tttggcgggt tttgccgtcg gcgtggtgga
     2581 aaaagagaat gtcattaccg gccgcagcat cggcgtaggc gatgtggtat tgggtttggc
     2641 ttccaacggc gcacattcaa acggctattc ccttatccgt aaaatcatcg aacgcgacaa
     2701 tcccgatctg gatgccgagt ttgataatgg caaaaccttg cgggaggctg ttattgcgcc
     2761 gacccgtctg tatgtgaaac cgattcttgc cgctttggaa aaatttacca ttaaaggtat
     2821 ggcacacatt accggcggcg gcattaccga aaacgtgccg cgcgtgttgc ctgaaaacac
     2881 ggttgcacaa atcgatgcta aatcgtggga attgcccaag ctcttccaat ggcttcaaaa
     2941 ggcgggcaat gtggaaaccc aagaaatgta ccgaaccttt aactgcggca tcggcatggt
     3001 cgttattgtt gctgccgaag atgccgatgc ggttcagggt ctcttgggtg aacaaggtga
     3061 aacagtttac cgtttaggtt tgatccgtga gcgtcaggga gacgagcatc aaacccaggt
     3121 tgcctgattg cttctatagc gaaatgccgt ctcaaacatg ggtttggacg gcatttttat
     3181 agagg
//
LOCUS       DAWHRA010000171         3108 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWHRA010000171 DAWHRA010000000
VERSION     DAWHRA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.171"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(317..793)
                     /gene="greA"
                     /locus_tag="WIB30_001950"
     CDS             complement(317..793)
                     /gene="greA"
                     /locus_tag="WIB30_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ3797955.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1185..2486
                     /gene="aroA"
                     /locus_tag="WIB30_001951"
     CDS             1185..2486
                     /gene="aroA"
                     /locus_tag="WIB30_001951"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ3797956.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDRGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADARYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVRAFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2529..3062)
                     /locus_tag="WIB30_001952"
     CDS             complement(2529..3062)
                     /locus_tag="WIB30_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ3797957.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIRTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          647 a          839 c          852 g          770 t
ORIGIN      
        1 tgacgaattt taggtttctg attttggttt tctgtttttg agggaatgac gggatgcagg
       61 ttttcttaac cctgcgtcct agattcccgc ttttgcggga atgacggcga cagggttgct
      121 gttatagcgg atgaacaaaa accagtacgg ggttgtctcg ccttagctca aagagaacga
      181 ttctctaagg tgctgaagca ccaagtgaat cgattccgta ctatttgtac tgtctgcggc
      241 ttcgtcgcct tgtcctgatt tttgttaatc cactataatt tcctgcgtgt gtcgggtgta
      301 tcgaaatcaa gccgaatcaa atatatcgga cttcgataat gtcgtattcg cgcacgccgc
      361 ccggggcttg gacttccgcc gtatccccct cttccttgcc gattaaggcg cgggcgatgg
      421 gtgagccgac atagattttg ccctgtttga tgtcggcttc gtcttcgccg acaatttggt
      481 aggtaacgtg ttcttccgtt tccaaatctt ccagcgtaac cgtcgtaccg aacacgattt
      541 tgccttcggc gtggatttcg gtcggattga tgatgtgggc aacggaaagt ttgtgttcca
      601 gctcggaaat gcggccctcg ataaagcctt ggcgttcttt ggcggcttcg tattcggcgt
      661 tttcggacaa atcgccgtgc gaacgggctt cggcaatcgc ttcgatcact tcgggacgcg
      721 ccacgctttt gagctgctgc aattcctgtt tcagcaattc cgcaccgcgt acggtcaggg
      781 ggattttttg catcggtctt ttttctccat attccggcac accggtttgc ggcagcaaac
      841 ataccgcgta ccgtcttgtt ttgtgcgtcc ggatattaaa ataaaaatac aagccgcccg
      901 gaaaatcggc ggcttgtctg tcgttgaaca gcggctattc taccaaattc tatgaaattg
      961 gcaatcgtac cgtaccgtcc gcaaacgcgc catgtccgca acaaaagctg aaaatatgcc
     1021 gacaaagaaa ttttagaaac aaaaaattta aaaataatca attttcggca taaaaaacca
     1081 catttacgga ctttaaaacc gaaaatgcca aacctgagat ttttcagacg gcatttgcac
     1141 cagtataatg caggctgttt ttatccctat attgacgttt tgccatgacc gaatccgtcc
     1201 gcctccccgt cgcccgtctc aaaccttcca ccgtcgccct gcccggctcc aaaagcatca
     1261 gcaaccgcac cctgctgctt gccgccttgt ccgacaatgc ttgcgaaatc cattccctgc
     1321 tcaaatccga cgataccgac cgtatgctcg aagcactcga taaactcggc gttcaaatcg
     1381 aatatcttgc cgaagaccgt ctgaaagtgc acggcacagg aggacgcttc cccaaccgca
     1441 ctgccgattt gtttttgggc aacgcgggca cggcgttccg cccgctgacc gccgcacttg
     1501 ccgttttggg cggcgattat catttgcacg gcgttgcgcg tatgcacgaa cgccccatcg
     1561 gcgatttggc cgatgcactg cggattgccg gcgccgatgt cgaatatctc ggcaaggaac
     1621 actatccgcc gcttcatatc ggcgaacgcc aagaccgcgg cgagcgcgtg attccgatta
     1681 aaggcaatgt gtccagccag tttctgaccg cccttttaat ggcgttgccg ctgaccgggc
     1741 aggcgtttga aatccgtatg gtcggcgagt taatttccaa gccctatatc gacattactt
     1801 taaaactgat ggcgcaattc ggcgtacagg ttatcaatga aggctaccgc gtcttcaaaa
     1861 ttcccgccga tgcgcgctac cacgcgcccg aacacttgca cgtcgaaggc gatgcctcca
     1921 gcgcgtccta cttcctcgca gccggtttga ttgctgccac gcccgtccgc gttaccggta
     1981 tcggcgcaaa cagcatacag ggcgatgtcg cctttgcccg cgagctggaa aaaatcggtg
     2041 cggacgtggt ttggggcgaa aacttcgtcg aagtctcgcg cccgaaagaa cgtgccgtcc
     2101 gggcctttga tttggatgcg aaccatattc ccgatgccgc gatgaccctc gccatcgtcg
     2161 cgcttgctac agggcaaacc tgcacgctgc gcaacatcgg ttcgtggcgc gtcaaagaaa
     2221 ccgaccgcat cgccgcgatg gcaaacgagt tgcgcaaact cggggcaaaa gtcgtcgaag
     2281 aagccgaagc aattcacatc accccgcccg aaacgccgac acccgacgcc gtcatcgaca
     2341 cgtacgacga ccaccgcatg gcgatgtgtt tctcgctggt ttcgctgttg ggcgtacccg
     2401 tcgtcatcaa cgatccgaaa tgcacccaca aaaccttccc gacttatttc gacgtgttct
     2461 catcgctgac cgaaacagcg gaataaggcg gcattttgcc gcgattccgg cgcagcgcgg
     2521 cgggcggctc attctgtaaa aaaagtatgt gcgccgaggt agtttttggc gtaaaacggt
     2581 gtggagagtt tttcggttcg gatggttttg ccgctgctgg gggcatggat gaattcgccg
     2641 ttgccgatat agagtccgac gtgtgagtag cggtgtgcgc cgccggtgtt gaagaatacg
     2701 aggtcgccgg ccttaaggcg gctgtcgggg attttgcggc ttgccgccgc catgtcacgg
     2761 gcggtgcgcg gcagcttgac gttgagggcg tttttgtaaa cgaattgaat catgccgctg
     2821 caatcgaagc cggttgcggt gctgctgccg ccccatttgt agggcgtgcc gatgagtccg
     2881 aggctgtgga gcatgagttc ctgcgagcct tgtgtgcggt cgatgtggct gatgcggacg
     2941 gcttggattt gccggactgt ctgtttgggt ttcggttggc ggtgtttgcc ggaggtcgtg
     3001 ccgcatgagg cgaggagcag tgcgctgaga cagaggaaaa gggttttgtc ggggggaaac
     3061 atggtttttc ctttgcgggt tcggatatcc gtctgaaggc gtttcaga
//
LOCUS       DAWHRA010000172         3078 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWHRA010000172 DAWHRA010000000
VERSION     DAWHRA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.172"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(93..1509)
                     /locus_tag="WIB30_001953"
                     /pseudo
     CDS             complement(93..1509)
                     /locus_tag="WIB30_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1569..2789)
                     /gene="argJ"
                     /locus_tag="WIB30_001954"
     CDS             complement(1569..2789)
                     /gene="argJ"
                     /locus_tag="WIB30_001954"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ3797958.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            complement(2856..>3078)
                     /locus_tag="WIB30_001955"
     CDS             complement(2856..>3078)
                     /locus_tag="WIB30_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ3797959.1"
                     /translation="GLVGQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRR
                     MAAYAGLGLTFNGVLTALIAPLLIPVLGF"
BASE COUNT          576 a          833 c         1048 g          621 t
ORIGIN      
        1 ctgtcgttca gacggcattg ttgctgaaat ttcaagcggc ggcggaacgg tattcaaatc
       61 aaggcggctg ccggcgtttg atcgggggcg ttttaatttt cagacggcat tcccgttttg
      121 ctgtttgctg tttgctgttt gcggtcttgc gggcgaattg ccggtttggg cggcggtttc
      181 ttgaagcacg cgctggcgga agcgcattcc cgatgcgtgg tagaacggac gcggcgaaaa
      241 ctggcgcgaa acctgcgagg cgaaaatgca ggcaatcagc atccaaaaca gcaggctttg
      301 tccgcccgtc atttccatga cgacgacggc ggaagtaatc ggggattgtg tcgcgcccgc
      361 cagaaatgcc gccatgcaga tgaggacgat gatgtttgca ccctgcgata tgtcggcgat
      421 ggcggcgata tgctcgccca aaaccgcgcc tatggtcagc gagggagtga aaatgccgcc
      481 cggaatgcct gcccaatagc tgaatacggt ggcgagccat ttggcggcgg cgagtccgaa
      541 gggggcttcg tagatgccgt gcagggcttg ggcggcttcg tggtagccag tgccgtaggt
      601 tttgccttgg tagaacgtgc cgagcagggc gagcagcagc cccatcagtg ccgccagcag
      661 cagcggacgg ttgcggatga agccgcgtat cttgcgcggt gcaaacgccg ccgcaccgcg
      721 atagagcaaa cgtccgaaca ggccgcccgc cgcgccgcaa accaggccgg acagtgcgac
      781 ccacagaaag atatgttcca atacgccgcc gttgaagccg gaaaaatacg ggttgttgcc
      841 ctgaatggcg acctgtatga aaccggaggc gagcacgccc aaaagaattt gcctctccca
      901 gcgcaacatg atgccgcgcc cgagttcctc aatggcgaaa atcacgcccg ccagcggcgc
      961 gttgaacgcg gctgccaaac cgcccgccgc gcccgccgcc atcaaatcgt tttcctgcat
     1021 ccctttgaat gccaagccgt gtttcttgca ccacgcgccc cacgcgccca tcactgccgc
     1081 gccgacctgc acggacggac cttcgcgtcc gatggacgcg ccgaacagca tacccaaaaa
     1141 ggttagcgga atcttcagca gcgtctgccc gaggcggatc agccgcgttt tctgtgcgcc
     1201 gtagggcagc gacagtgagg cgatgacctg cgggatgccg ctgccggcgg tgaagggggc
     1261 gaatttgcgt gtgagccacg caataagcgg taaacccaaa ggaagcgcga cccacgcgaa
     1321 ccacgggtat tgttgaacca gtttggcgtt cagttccagc gcaaaatcgg caagatgggc
     1381 aaaaaacagc gcggtcaggg cgacgagtgc cgaacctgcc aaaaggaaca gaaaggcgat
     1441 gcttttgcgc gacaggcggc gggtttggcg gattttgcgg gcgaggcggc gggggaatgt
     1501 ggaggtcatg gtatgttgtc tggcggttca gacggcattt tatgtatgcc gtctgaagcc
     1561 gtgtcgggtc aggaacggta gtcggcgttg atggaaacgt atccgtgcga caggtcgcag
     1621 gtatagacgg tggcggcggc ttgtccgcga tgcagcttga tgcggacggt gatttcggcc
     1681 ttcgacatca ccgcctgccc ttgtgcttcg gtgtagcttg cggcgcgtcc accgtgttcg
     1741 gcaaccaaaa tatcgtcgag atacatttcc acgaggtcgg tatcgaggtc ggcaacgccg
     1801 gcataaccga tggcggcgag cagcctgccg aggttggggt cggaggcgaa aaaggcggtt
     1861 ttgaccagcg gcgaacgtgc cacggcgtag gcggcttggc gggcttcgtc gcgggttttg
     1921 gcgttttcga cgcggacggt gatgaacttg gtcgcacctt cgccgtcgcg gacgatggct
     1981 tgggcgagtt cgagcgcgag gctgcacaac aattctttga gttgggcgta acgcgggtcg
     2041 gcgatgttgt cgatttcgct ttggctgttt ttgccggtgg cgatgatgac gaagctgtcg
     2101 ttggtgctgg tgtcgccgtc aacggtgatg gtgttgaagg tttcgtcggc gatttcctgt
     2161 gtcatcagtt ggaggacggg ttgggaaact ttggcatcgg tggcgatgaa accgagcatg
     2221 gtcgccatat tgggatgaat catgcccgag cctttggcga tgcccgtggc gcggacggtg
     2281 tgtttgtcgc cgaccttgcc ttcgcgcgag gcggctttgg gcacggtgtc ggtggtcatg
     2341 atggcgcgtg ccgcttcgtt ccagaaggca ggctgcattt tgggcagcgc ggcgatgatt
     2401 ttgtctgcgg gcagcggttc gagaatcacg ccggtggaga agggcagcac ctggttcggt
     2461 ttgcagccga tttgccgggc ggcggcggca cacactgcca aagcatcgat tctgccctgt
     2521 gcgcccgtac ccgcgttggc gttgcccgtg ttgatgacga gggcgcgcac gccgtcttcg
     2581 tcgaaaaggt gcgatttggc gatgtggacg ggcgcggcac agaaacggtt ggtcgtgaag
     2641 actgcaccga cggtgctgcc ggcggctacg gcaatcagtg tcaggtcggt atgcccgggc
     2701 ttcttcacgc ctgcttgggc ggtgtagagg gcaatgccgt cgatgtcggg cagttgttcg
     2761 gcggtttttt cggtcaggtt gacagccata acggttctcc ttgcgagtgt gtcggcgttc
     2821 agacagcatg ggctgaaatg tcgtctgaaa cgggttcaga accccaaaac ggggatgagc
     2881 agcggcgcaa tcagcgcggt cagcacgccg ttgaacgtca gccccagccc cgcgtatgcc
     2941 gccatgcggc ggctgcgttc gagcgaggcg gcaatcccca tcgcgtgcga ggcagtgccg
     3001 agcgacatac ccacggacga gggcatgacg accgtgttct tcagcatttt gtaaccggca
     3061 atctgtccga ccagaccg
//
LOCUS       DAWHRA010000173         3035 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWHRA010000173 DAWHRA010000000
VERSION     DAWHRA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.173"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(81..563)
                     /locus_tag="WIB30_001956"
     CDS             complement(81..563)
                     /locus_tag="WIB30_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ3797960.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(716..877)
                     /locus_tag="WIB30_001957"
     CDS             complement(716..877)
                     /locus_tag="WIB30_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3797961.1"
                     /translation="MSGQPEKHHTSPIEDKRKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(951..1412)
                     /gene="recX"
                     /locus_tag="WIB30_001958"
     CDS             complement(951..1412)
                     /gene="recX"
                     /locus_tag="WIB30_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ3797962.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1485..2195)
                     /locus_tag="WIB30_001959"
     CDS             complement(1485..2195)
                     /locus_tag="WIB30_001959"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ3797963.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETA
                     TGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            2602..>3035
                     /locus_tag="WIB30_001960"
     CDS             2602..>3035
                     /locus_tag="WIB30_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ3797964.1"
                     /translation="MSSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRV
                     LEVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCC
                     VAGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGE"
BASE COUNT          659 a          699 c          891 g          786 t
ORIGIN      
        1 ctatatttgc ttgatttcat ccgtttgcaa tggaaacgga tggcgcgatg tgcctgtctt
       61 tcagacggca tagtccgccg tcagcagccg caggacatgt cttcagaggc ttgcaggctg
      121 atgtctctgc gttttttggc tttttcgtag cggcagcgtt ggcggtcggt caggatttcc
      181 agcggaggga cagggacggg tttgccgtct ttgaccgcaa ccatcgtgaa gtagcagctg
      241 ttggtatggc ggatttcgcc ggtgcggatg ttttgtgctt cgacacggat gccgatttcc
      301 atcgaggtgc gtcccgtgta gttgacagcg gcgtaaaagg taaccaaatc gccgatgtgg
      361 atgggttctt taaacaggac tttgtcaacc gacagggtaa cgcaataatt gccgctgtaa
      421 cggctggcgc aggaataggc gacttggtcg agcaggagca ggagttcgcc gccgtgtacg
      481 ttgccgctga aattggcggt gtccggcatc atcagttcgg acataatgag ttcgtgcgaa
      541 ggcagttggc gttgttgtgt catggtgtac ctgttttaag aaagggtgtt ttgaaatggg
      601 tgcggggaaa ttttagggaa tggggaagtg gaattcaagc taaaaaaagc tatggcggga
      661 tagtctgcag aaatgggccg ccgatgccgt ctgaagcctt cagacggcat tttgtttagg
      721 aaagccaaaa acggaagacc aggaaaaaca cgagtgccaa tacggcgacc caaacgacga
      781 gcatgaatcc ggcaactgcc cgtcccatac ggtaaaccgg gtttttgcgt ttgtcttcga
      841 tgggggaggt gtggtgtttt tcgggttgtc cggacatttt gttctcaatc ggaaaagttg
      901 gaaacgggcg taaggttatg ttgccgtgca aaaagattca agaaggaagg tcagcagccg
      961 tcttcccaat tttcgtccca agcgtgtttc aatgccgtct gaacggtatc ggcatcgaat
     1021 ccgcgatagg cgaggaaacg cgcctgtttt tgtttttctt tgaggtcggc ggcggggtgt
     1081 ttgaattttt tacgcaacac ggctatggcg gcctgttttt cgcttgagcg gtcgggaagc
     1141 aggttgcggc tggtttcttc atctatgccc tgttgcgcca aagcctgttt cagcctcaat
     1201 gaaccgtgtt tgcggctttt gctgcggata taggcttcgg catagcgcaa atccgactgc
     1261 cagttgcgtt cggcaaattc gtttaacacg ttttccaact cctcttcgct ttcggcgtgc
     1321 ggtgcaagtt tgcgtttcag accgatgcgg ctgacttctt ggcgcgagag gatgtccatc
     1381 gcgcgggcgc gtagggattt ttgcggtttc atggcggcgg tttcggcacg gaaagattca
     1441 gacggcatgg gaaacggatt ccgatgccgt ctgaacagat tgttttattc ttcgtcttta
     1501 tttttctgcg gttgcaggtt ttcgtaaatt tcgggcagcg agccgataat ccagtcgggg
     1561 cgggtcgcat cgtcttgcga gagcagcgtc atatcgccgt aaccgaaggt aacgccgacg
     1621 ctcaggcagc cggcggcttt ggcggcgatg atgtcgttgc gcgagtcgcc gaccataacc
     1681 atgtttgcaa catcgatacc caaaacttcg gcggcgtgcc gcagcggcag ggggctgggt
     1741 tttttctcgg gcaggctgtc gccgccgagt atcaggctga agtagtcggc aagtccgagc
     1801 tgtttcaaca gttcgacggc aaggatttcg tttttattgg taatgactgc cagcgggatg
     1861 cccaaagatt tgagcaatgc cagcccggtt gcggtttcgg gataggggcg ggtgaagacg
     1921 ctcaaatggt cgcggtagta tttcatatag gatacgaaac ctttttccca cagttcggaa
     1981 tcggcttcgc ggtcgcggtc gttggagagg acgcggtgaa ccagtttgcc gatgccgtcg
     2041 cccacatagc tttcgaccac tttggcgggc agcggtttca taccgagttg ttccaacatc
     2101 gcttctgcgg cggcggcaag gtcggggacg gaatcgcaca gtgtgccgtc caaatcgaag
     2161 gcgacggctt ggacgtgttc gatggctgca ttcataatct gatttcctgt cgggttgaaa
     2221 gggtttattt taacatcgtt tgcacagggc ttctaatgta tagattgtta acaatcgcaa
     2281 caggttttgc aggcggtgtt ttcggatttc ggggcggaat cgggcaaggg tttgaaatcc
     2341 cagcgatgtt ccaattaaat ttttaaaaaa taaaaggatg cctatatctt tatttaattt
     2401 gacatttatt tttcggacgc gcgcgctgct ttttcaggcg gagtccgggc tgattccgtg
     2461 cggcttgaaa aataaaatca tatatagatt aatcaaatcg ggcttatgcc gactacacgc
     2521 tattacattt tgtcgggaaa ttggttatag taatgagcga aatgcacgcc cctgaaaatc
     2581 tttttgtgaa aaggaagcaa aatgtcgtct gaagcccttg caaaagaaac cctcaatccg
     2641 tttgaaatcg cgcgcaaaca ggtcaaaacc gcctgcgacc ggctgaaaac cgatccggcc
     2701 gtttatgaaa tcctgaagag tccgacccgc gtgttggaag tcaatttccc cgtcaaactc
     2761 gacgacggca cggtcaaaac cttcacgggc taccgttcgc aacacaacaa cgccgtcggc
     2821 ccctataaag gcggcgtgcg cttccatccc ggagtgaatc tggacgaagt caaagccctg
     2881 tcgatttgga tgaccatcaa atgctgcgtt gcgggtattc cttacggcgg cggcaaaggc
     2941 ggtattacct tggatccgcg cgattattcc gaggcggaac tggaacgcat cgctcgcgct
     3001 tattccgaag ccatttctcc gctgattggc gagaa
//
LOCUS       DAWHRA010000174         3010 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWHRA010000174 DAWHRA010000000
VERSION     DAWHRA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.174"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1014
                     /locus_tag="WIB30_001961"
     CDS             <1..1014
                     /locus_tag="WIB30_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ3797965.1"
                     /translation="GEIAIAEQIAKIMPSVERLRLVSSGTEATMTAIRLARGFTGRDK
                     IIKFEGCYHGHSDSLLVKAGSGLLTFGNPSSAGVPADFTKHTLVLEYNNIAQLEEAFA
                     QSGNDIACVILEPFVGNMNLVRPTEAFVKALRGLTEKHGAVLIYDEVMTGFRVALGGA
                     QSLHGITPDLTTMGKVIGGGMPLAAFGGRKDIMECISPLGGVYQAGTLSGNPIAVAAG
                     LKTLEIIRREGFYENLTALTSRLADGIAAAAKAHGIEFTADSVGGMFGLYFASHTPRN
                     YADMARSNIDAFKRFFHGMLDRGIAFGPSAYEAGFVSAAHTPELINETVAVAVEVFKA
                     MAE"
     gene            complement(1230..1502)
                     /locus_tag="WIB30_001962"
     CDS             complement(1230..1502)
                     /locus_tag="WIB30_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ3797966.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(1513..2076)
                     /gene="orn"
                     /locus_tag="WIB30_001963"
     CDS             complement(1513..2076)
                     /gene="orn"
                     /locus_tag="WIB30_001963"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ3797967.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPERATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2094..2981)
                     /gene="prmA"
                     /locus_tag="WIB30_001964"
     CDS             complement(2094..2981)
                     /gene="prmA"
                     /locus_tag="WIB30_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ3797968.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQIEELSGIYSQWF
                     DLDPAETEEGWARLSGTKR"
BASE COUNT          605 a          794 c          861 g          750 t
ORIGIN      
        1 ggcgaaatcg ccattgccga acaaatcgcc aaaatcatgc cgtccgtcga acggctgcgc
       61 ctcgtcagct ccggtaccga agcgacgatg accgccatcc gtttggcacg cggttttacc
      121 ggtcgcgaca aaatcatcaa gtttgaaggc tgctaccacg ggcattccga cagcctgctg
      181 gtgaaagccg gcagcggcct gctgacgttc ggcaatcctt cttccgccgg tgtgcctgcc
      241 gactttacca aacacacttt agtccttgag tacaacaaca tcgcccaatt ggaagaagcc
      301 tttgctcaaa gcggcaatga cattgcctgt gtgattttgg agccttttgt cggcaatatg
      361 aaccttgtcc gcccgaccga ggcctttgtc aaagccttgc gcggattgac cgaaaaacac
      421 ggcgcggtgt tgatttacga cgaagtgatg accggcttcc gcgtcgcgct cggcggcgcg
      481 caatcgctgc acggcatcac gcccgacctg accacgatgg gcaaagtcat cggcggcggt
      541 atgccgcttg ccgcgttcgg cggacgcaaa gacatcatgg aatgtatttc cccgctgggc
      601 ggcgtgtatc aggcaggcac attatcaggc aacccgattg ccgtcgccgc cggcttgaaa
      661 acgctggaaa tcatccggcg cgaaggcttc tatgaaaacc tgactgccct gaccagccgc
      721 ctcgccgacg gtattgccgc cgccgccaaa gcgcacggta tcgaattcac cgccgacagc
      781 gtgggcggta tgttcggtct gtattttgcc agccatacgc cgcgaaacta tgccgatatg
      841 gcgcgatcca atatcgacgc tttcaaacgc ttcttccacg gcatgctcga ccgcggcatt
      901 gcctttggcc cgtccgctta tgaagcaggt ttcgtttccg ccgcgcatac gcccgagctg
      961 attaacgaaa cggttgcggt tgcggttgaa gtgttcaagg cgatggctga atgatgtttt
     1021 gacggacaga gtttctctgt tcgatttgtt tggcagattg aagtaagaat gcacaccgcc
     1081 gtcattcccg cgcaggcggg aatccagacc tttcagtttc tgtaatgatt gaaaataacg
     1141 tgccgaaatc agccgcaccc ggcaggaagc cgccgccacc tgaaacaatg ccgtctgaag
     1201 ccttcagacg gcattttatc ggaagcgcat cagaaaaaac ggatagcgac atatgcggta
     1261 aaagcaaggg caattacata gataaacccc tttataaatt caaaagtctc atctatatcg
     1321 ctatattttt ctttcagttc gtcagatttt cgataacgtt tcattttcaa gaccacttca
     1381 tgaccaacaa gcccgacctt ccggattccc gcctgcgcgg gaatgacggg cgtgtatatt
     1441 tttgatttca atctgctgta aaaatgccgt ctgaaatgca ggatttgcgc ttcagacggc
     1501 attgtttgtt tcttattgcg cttcggctct cggggcggaa atcagaaagt gttcgcggta
     1561 gtggcgcatt tcttcgatgc tctccaaaat gtcgtccaat gccttgtgcg aaccgcgttt
     1621 gaccacgctt ttggcaacgg gcggattcca gcgtttggca agctctttga gcgtggaaac
     1681 gtcgaggttg cggtagtgga agtagttttc cagtttcggc atatatttga ccataaaacg
     1741 ccggtcttgg tggatggagt tgccgcacat cggcgtggcg cgttcgggta cccattccga
     1801 cataaagtcc agcagtttct gttcgacttc ggcttcggta tgcgacgatt cgcgtacgcg
     1861 ctgcgtcagc cccgtcctac cgtgtgtggc ggtgttccac tcgtccatat tgtcgagcag
     1921 ctcgtcgctt tggtggatgg cgtaaacttc ggattgcgcc aatacattca aatccgagtc
     1981 ggtaataatc atcgcgactt cgataatgcg gtcggtttcg ggattaagcc ccgtcatttc
     2041 catatcgagc cagcataggt tgtttttatc ctgcatgatg tttcctttgc ttgtcagcgt
     2101 tttgtcccgc ttaatcgcgc ccatccttct tcggtttccg caggatcgag atcgaaccat
     2161 tggctgtaaa tgccgctgag ttcttcgatt tgttcgtcca acaaaccgga taacacgatg
     2221 cggccgcctt gtttggtgcg ggcggcaagc atttcgccga gcatacgtaa agggttggcg
     2281 aggatgttgg caacaactac gtcgaattgc ccttgaggca ggccgtcggg caggaagaat
     2341 tgtgcatcga cgttgttctg ttcggcgttg tccttgccgg cgcggacggc ttgttcgtca
     2401 atatcgacgc cgacggcaga acctgcgccg agtttgaggg cagcgatggt caggatgccc
     2461 gaaccgcagc cgtagtcgag gacgctttcg ccgtttttga gttgcgtatc cagccatttg
     2521 aggcagaggc gcgtggttgg gtggctgcca gtgccgaagg cgaggccggg atcgaggcgt
     2581 aggttgacgg cagtgccttc gggggcttcg tgccaagagg gggtaatcca caggcggtcg
     2641 gaaattcgga tggggtcgaa ttgcgattgc gtgagacgca cccagtcttg gtcttcgatg
     2701 gtttcgccgg tgtatgccaa gtcttttaac ccgcattctt gtgcggcgat ttggatgatg
     2761 gcggcggctt cgtcgtgttc gccgaacagg gcgatgactt tgctctgctg ccagatttgt
     2821 tcggcgggca taccgggttc gccgaaaatc gcctgttcgt tttgcgtgcc ggcgtaggca
     2881 tcttcgatgg cggcggagag tgcgccgtgt tccatcagcg cgtcggcgag gcgttcggcg
     2941 acggcatcgt tgacgttgac ggtgatttgt tggtagggca taacgggctt tcttggaaat
     3001 atagtggatt
//
LOCUS       DAWHRA010000175         2924 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWHRA010000175 DAWHRA010000000
VERSION     DAWHRA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.175"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(102..1616)
                     /gene="katA"
                     /locus_tag="WIB30_001965"
     CDS             complement(102..1616)
                     /gene="katA"
                     /locus_tag="WIB30_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ3797969.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            1964..2815
                     /locus_tag="WIB30_001966"
     CDS             1964..2815
                     /locus_tag="WIB30_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ3797970.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDPDEFSDAGFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHALTALKKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
BASE COUNT          629 a          774 c          811 g          710 t
ORIGIN      
        1 cggtcaaacg acgtttcaga cggcattttt atgcccggtt atttccggta gcggacggcg
       61 cgggacagga tttcttcaat ttccatccac ataatgcccc cttacagcaa accgggctgg
      121 ccgagtgcgg ggtcggtcgc gcgggcggct tgtgcgtctt cgacagtcag tccgagggct
      181 tttgccacgc cttcgccgta tgccgggtcg caacggtagc agttgcggat atggcggtat
      241 ttgatgaagt cgggcgcgtc gcccattgcg gcggcggtgt tgccgaacaa tgcctgtttc
      301 tgcgcgtcgt tcatcaggtt gaacagggcg cgcggttggc tgaaatagtc gtcatcgtct
      361 tggcggtagt cccagtgtgc cgcgtcgccg ttgattttca aaggcggttc ggcgaagtcg
      421 ggttgttgct gccattggcc gaagctgttg ggttcgtagt gcggcaggct gccgtagttg
      481 ccgtcggcgc ggccttgccc gtcgcgctgg ttgctgtgaa cagggcaacg cggacgattg
      541 acgggaattt ggcggaagtt tacgcccaaa cggtagcgtt gtgcgtcggc gtaattgaac
      601 aaacgcgctt gcagcatttt atctgggctg gcgccgacac cgggaacgag gttgctcggt
      661 gcgaaggcgg attgttccac atcggcgaag aagttttcgg gattgcggtt caactcgaat
      721 tcgcccactt caatcagcgg atagtctttt ttcggccaaa ctttggtcaa gtcaaacgga
      781 tgataaggta ctttttccgc gtctgcttca ggcatgactt ggatgtacat cgtccatttc
      841 ggaaactcgc cgcgttcgat ggcttcgtat aagtcgcgct gatggctttc gcggtcgtcg
      901 gcgatgattt tggcggcttc ttcgttggtc aggtttttaa tgccttgttg ggtgcggaaa
      961 tggaatttca cccaaaaacg ctcgcctgct tcgttccaga agctgtaggt atgcgaaccg
     1021 aagccgtgca tatggcggta gccggcgggg atgccgcggt cgctcatcac gatggtaact
     1081 tggtgcagtg cttcgggcag cagcgtccag aagtcccagt tgtttgtggc agagcgcata
     1141 ttggtgcgcg ggtcgcgttt gacggctttg ttcaggtcgg ggaacttacg cgggtcgcgc
     1201 aggaagaaca cgggcgtgtt gttgccgacc acatcccagt tgccttcttc ggtataaaat
     1261 ttcaaggcaa aaccgcggat gtcgcgttct gcatcggctg cgccgcgttc gcctgccacg
     1321 gtggtgaaac gggcgaacat ctcggttttt ttgccgactt cgctgaagat tttggcgcgg
     1381 gtgtatttgg tgatgtcgtg cgttacggta aacgtaccga acgcgcccga acctttggcg
     1441 tgcatacggc gttcggggat gacttcgcgc acgaagtcgg cgagtttttc attcagccac
     1501 aaatcctgcg ccagcagagg gccgcgagga ccggcggtca ggctgttttg attgtcggca
     1561 acaggcgcgc cgttgttcat ggtcagatgg gttacagggc atttggaggt agtcatcgct
     1621 cttgttcctt ttctcaggtt ggtcaaatgg gggtaaacgg cttacagtac gatttggcgg
     1681 aaagcgtatt cgtaaccggt ttcttgattg caataaattt cttgaatcga cattttattt
     1741 cccttttgta aaaactatgg atgcgactat acgccaagat tttcgctatt aaaactatga
     1801 aatcgattta atattattat aagcaatcgg ttcttgattt tcgtttgttt tttgttatcg
     1861 aacggaatcc gaacccgctc attaaaacca tttataatgc aatgacgctt tgcggcattt
     1921 tttgcgccga caggctgaaa ataacaattt tccccacatt atcatgacct tacttggaat
     1981 aaagctcaaa cagacccagc agctcaacca gcggctgcaa caatctttgc gcgtattgca
     2041 gatgtcgggt atcgaacttg aacgcgaggt cgaaaactgg ctgtcggaca accccctgct
     2101 cgaacgcaaa gacccagatg aattttccga tgccgggttt tcccattaca ctgcgcccgc
     2161 gcgccaaatc ggcggagacg aaggcgaaga tatgctgtcc aacatcgccg gcgagcagga
     2221 tttcaagcaa tacctgcacg cgcaagtatg cgaacacccg ctttccgacc aagaagccgc
     2281 ctgtgtccac atccttatcg atttccttga cgagcagggt tatctgaccg acagcatcga
     2341 agacatcctc gaccatacgc ccttagagtg gatgttggat gaagcaatgc tgcaacacgc
     2401 gctgaccgca ttgaaaaaat tcgacccggc aggcgtggcc gccgccgatt tgaacgaatc
     2461 gctgatactg cagatagaaa gattgggcga atgtgctgcc aaacccgccg ccctgcatat
     2521 cgtccgaaac gccctcgaca gcattgacgg caaccgcagc caaaccctcg cacgaataaa
     2581 aaaacacctg ccccaaaccg acagcggcac actcgaagcc gcactcgacc tcattgcttc
     2641 gctcaatccc tttcccgccg ccggttttgc ctcgtccacg cccacgccgt attctgacga
     2701 ggcgctcgcc aacctgctgg ctttccgcgg catggaggtt tctcgccgca ccattgccaa
     2761 atacagagaa tcctttgaga ttccggcagc acacaaacgc aaaaccgcag aataattgcc
     2821 gaataatctt ataaagacaa caaaccaaaa gccggcattt ctgcgaaagc gggaatgccg
     2881 aatccgtccg cgcggaaacc tgcatcccgt cattcccgcg aaag
//
LOCUS       DAWHRA010000176         2691 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWHRA010000176 DAWHRA010000000
VERSION     DAWHRA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.176"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..281
                     /locus_tag="WIB30_001967"
     CDS             <1..281
                     /locus_tag="WIB30_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ3797971.1"
                     /translation="HPSVPATAQVLEHMRGSPYEETINRIFRSALQSEEMEGGGEEDC
                     ENFQIGIKKLLNELKYSQIETLKQKSLQSGLNESEKKLLLSLLTAKQN"
     gene            476..2404
                     /gene="rpoD"
                     /locus_tag="WIB30_001968"
     CDS             476..2404
                     /gene="rpoD"
                     /locus_tag="WIB30_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ3797972.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            2572..>2691
                     /locus_tag="WIB30_001969"
     CDS             2572..>2691
                     /locus_tag="WIB30_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ3797973.1"
                     /translation="MIAKHIDRFPLLKLDRVIDWQPIEQYLNRQKTRYLRDHRG"
BASE COUNT          849 a          783 c          586 g          473 t
ORIGIN      
        1 accatccttc cgtgccggca accgcacagg ttttggaaca tatgcgcggt tcgccctacg
       61 aagaaacaat caaccgcatc ttccggtcgg cccttcaatc ggaagaaatg gaaggcggcg
      121 gcgaagaaga ttgcgaaaac ttccaaatcg gcatcaaaaa actgctcaat gagttaaaat
      181 acagccaaat cgaaacatta aaacaaaaaa gcctgcaatc cggcttaaat gaaagcgaga
      241 aaaaactttt gctgtcgctg ctgaccgcaa aacaaaattg accggcggat tccgccatcc
      301 gtaaaccgtt atgccgtctg aaaagcattc accccggctg caacaacgac acctgcagaa
      361 cacccatccc caaaagcctt caagccttca gacggcatca gagtacccta ctctgccacg
      421 ccttcaggtg cgtccaaacg caaaccgtcg gcatcttacc aacagaaagc agacaatgtc
      481 cagaaaccaa aatcacgaag aatatcaaga cgacacccgt ccgttaagca ttgaagagca
      541 acgcgcgcgc ctgcgtcagc tcatcatcat gggtaaagaa cgcggctaca tcacctactc
      601 cgaaatcaac gacgccctgc cagacgatat gtctgatgcc gaccaaatag acaatatcgt
      661 cagcatgatt tccggtttgg gcatccaagt taccgaacac gcccccgatg cggaagacat
      721 attgttaagc gacaatgccg ccgttaccga cgatgatgcc gtcgaagaag ccgaggccgc
      781 cctttccagt gcagattccg agttcggcag aaccaccgac cccgtccgta tgtatatgcg
      841 cgaaatggga caggtcgacc tgctgacccg cgaagacgaa atcatcatcg ccaaaaaaat
      901 cgaaaacgcc ctgaaaaata tggttcaggc catctccgcc tgcccgggat ccattgctga
      961 aatcttagaa ctcatcgaaa aaatccgcaa agacgaaatc cgcgtcgacg aagtcgtaga
     1021 agccattatc gacccgaatg aagtattgct caacgaattg ggcttggggc acttggaaac
     1081 cacagcaccc gagaaacctt ccaacgacaa ttcggacgaa aacgaagacg acgaagaatc
     1141 ggaagaagat gcggatgaaa tctcggcagc caatctcgcc gaattgaaac aaaaagtcat
     1201 cggccacttt gcccaaatcg aaaaagacta caaaaaaatg atcggtcgtt tggaaaaaca
     1261 ccacagccgg cacaaagact atctcgccta ccgcgacgcg attgccaaca aactgctgga
     1321 agtccgtttc gccacccggc aaatcgacag cctcagcagc agcctgcgcg gaaaagtaga
     1381 aaacatccgc aaactcgaac gcgaaatccg cgacatctgc ctcgaccgcg tccatatgga
     1441 gcgcgactac ttcatccaaa acttcctgcc cgaaatcacc aatctgcaat ggattgaaga
     1501 agaaatcgcc aaaggcaggg tttggagcga cgcgctcgac cgcttccgcc acgccatcct
     1561 cgaaaaacaa accgagttgg cagatatgga aaaagaaacc cgcatttcca tcgaagagtt
     1621 gaaagaaatc aacaaaaata tggtgtcgag cgaaaaagaa accgcagccg ccaaacagga
     1681 aatgattcag gcaaacttgc gcctcgtcat ttccattgcc aaaaaataca ccaaccgtgg
     1741 cttgcagttc cttgatttga ttcaggaagg taacatcggc ctgatgaaag cggtcgacaa
     1801 gttcgaatac cgccggggct acaaattctc cacctacgca acctggtgga tccgccaggc
     1861 aatcacccgt tcgattgccg atcaggcgcg taccatccgc attccggtac atatgattga
     1921 aaccatcaac aagatgaacc gcatctcgcg ccaacacctt caagaaaccg gcgaagaacc
     1981 cgattccgcc aaacttgccg aactgatgca gatgcccgaa gacaaaatcc gcaaaatcat
     2041 gaaaatcgcc aaagagccga tttcgatgga aacccccatc ggcgacgacg acgattcgca
     2101 cttgggcgac ttcatcgaag atgccaacaa tgttgcgccg gccgatgcgg caatgtacac
     2161 cagcctgcac gaagtaacca aagaaatcct cgaaagcctg acaccgcgtg aggcaaaagt
     2221 cctgcgtatg cgtttcggca tcgatatgaa caccgaccac acgctggaag aagtcggcag
     2281 acagtttgac gtaacgcgcg aacgcatccg acaaatcgag gcaaaagcac tccgcaagct
     2341 gcggcatccg acaagaagcg accgtttgag aagtttcttg gacagcgaag acagcaagct
     2401 gtaaaccaaa aaaccgcagg tttcaaatac ctgcggtttt ttcttacaca ataaacaacg
     2461 cttccacata tcccacactc ctatcccgag acctttgcaa aatcccccaa aatcccctaa
     2521 attcccacca agacatttag gagcaccttc ttccaacaaa ccgcccaagc catgattgcc
     2581 aaacacatcg accgtttccc gctattgaag ttggaccggg tgattgattg gcagccgatc
     2641 gagcagtacc tgaaccgtca aaaaacccgt taccttagag accaccgcgg c
//
LOCUS       DAWHRA010000177         2675 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWHRA010000177 DAWHRA010000000
VERSION     DAWHRA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.177"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            446..2611
                     /locus_tag="WIB30_001970"
     CDS             446..2611
                     /locus_tag="WIB30_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ3797974.1"
                     /translation="MNAPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFTVGSEDSRINIKR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNH
                     TRITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFEISDKKKGADGKEVD
                     VDDAQKEKNRANEKIVHAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTH
                     DGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGT
                     KAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNH
                     GYELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFK
                     NPNLEIGWRGRYVQKAVGSILVAGQKDRNGKLENVVRKGFGVNDVFANWKPLGKDTLN
                     VNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          742 a          766 c          618 g          549 t
ORIGIN      
        1 tccagttgtt tctgataaat tcttgcagct ttgagttcct agattcccgc tttcgcggga
       61 atgacgcgga aaggttgctt ttcgtcattc ccgcgcaggc ttcgtcattc ccgcgcaggc
      121 gggaatctag gttcgttcgg tttcagtcat ttccgataga ttcctgccgc gttaggggtt
      181 ctagattccc gctttcgtgg gaatgacgcg gaaaggttgc ttttcgtcat tcccgcattt
      241 gttaatccac tatatttccg ccgtttttac atttccgaca aaacctgtca acaaaaaaca
      301 acacttcgca aataaaaacg ataatcagct ttacacaaat cccccctctc gttaatataa
      361 acaaaaataa ttattatttt tcttatcctg ccaaacctta acggtttgga tttacttccc
      421 ttcatacact caaaaggacg aacaaatgaa cgccccatta ttccgcctca gcctgctctc
      481 gcttaccctg gcggcaggtt ttgcccacgc ggcagaaaat aatgccaagg tcgtactgga
      541 taccgttacc gtaaaaggcg accgccaagg cagcaaaatc cgtaccaaca tcgttacgct
      601 gcaacaaaaa gacgaaagca ccgcaaccga tatgcgcgaa ctcttaaaag aagagccttc
      661 catcgatttc ggcggcggca acggcacgtc ccaattcctg acgctgcgcg gcatgggtca
      721 gaactctgtc gacatcaagg tggacaacgc ctattccgac agccaaatcc tttaccacca
      781 aggcagattt attgtcgatc ccgctttggt taaagtcgtt tccgtacaaa aaggcgcggg
      841 ttccgcctct gccggtatcg gcgcgaccaa cggcgcgatt atcgccaaaa ccgtcgatgc
      901 ccaagacctg ctcaaaggct tggataaaaa ctggggcgtg cgcctcaaca gcggctttgc
      961 cagcaacgaa ggcgtaagct acggcgccag cgtattcgga aaagagggca acttcgacgg
     1021 cttgttctct tacaaccgca acgatgaaaa agattacgaa gccggcaaag gtttccgcaa
     1081 tgtcaacggc ggcaaaaccg taccgtacag cgcgctggac aaacgcagct acctcgccaa
     1141 aatcggaaca accttcggcg acggcgacca ccgcatcgtg ttgagccata tgaaagacca
     1201 acaccggggc atccgcactg tgcgtgaaga atttaccgtc ggcagcgaag attcacggat
     1261 aaatattaaa cgccaagccc ctgcttaccg cgaaaccaca caatccaaca ccaatttggc
     1321 gtacacgggt aaaaacctgg gctttgtcga aaaactggat gccaacgcct atgtgttgga
     1381 aaaagaacgc tattccgccg atgacagcgg caccggctac gcaggcaatg taaaaggccc
     1441 caaccatacc cgaatcacca ctcgtggtgc gaacttcaac ttcgacagcc gccttgccga
     1501 acaaaccctg ctgaaatacg gtatcaacta ccgccatcag gaaatcaaac cgcaagcgtt
     1561 tttgaactcg aaatttgaga tctccgataa gaaaaaagga gccgacggaa aagaagtcga
     1621 cgtagatgat gcacaaaaag aaaaaaatcg tgccaatgaa aaaattgttc acgcctacaa
     1681 actgtctaac ccgaccaaaa ccgataccgg cgcgtatatc gaagccattc acgagattga
     1741 cggctttacc ctgaccggcg ggctgcgtta cgaccgcttc aaggtgaaaa cccatgacgg
     1801 caaaaccgtt tcaagcagca accttaaccc gagtttcggt gtgatttggc agccgcacga
     1861 acactggagc ttcagcgcga gccacaacta cgccagccgc agcccgcgcc tgtatgacgc
     1921 gctgcaaacc cacggtaaac gcggcatcat ctcgattgcc gacggcacga aagccgaacg
     1981 cgcgcgcaat accgaaatcg gcttcaacta caacgacggc acgtttgccg ccaacggcag
     2041 ctacttctgg caaaccatca aagacgcgct tgccaatccg caaaaccgcc acgtctctgc
     2101 cgctgtccgt gaagccgtca atgccggtta catcaaaaac cacggttacg aattgggcgc
     2161 gtcctaccgc accggcggcc tgactgccaa agtcggcgta agccacagca aaccgcgctt
     2221 ttacgatacg cacaaagaca agctgttgag cgcgaatcct gaatttggcg cacaagtcgg
     2281 ccgcacttgg acagcctccc ttgcctaccg cttcaaaaac ccgaatctgg aaatcggctg
     2341 gcgcggccgt tatgttcaaa aagccgtggg ttcgatattg gtggcaggtc aaaaagaccg
     2401 caacggcaaa ttggaaaacg ttgtacgcaa aggtttcggt gtgaacgatg tcttcgccaa
     2461 ctggaaaccg ctgggcaaag acacgctcaa tgttaatctt tcggttaaca acgtgttcaa
     2521 cacgttctac tatccgcaca gccaacgctg gaccaatacc ctgccgggcg tgggacgtga
     2581 tgtacgcttg ggcgtgaact acaagttcta aaacgcacat cccgaaaaaa tgccgtctga
     2641 aagcctttca gacggcatct gtcctgataa tttga
//
LOCUS       DAWHRA010000178         2661 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWHRA010000178 DAWHRA010000000
VERSION     DAWHRA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.178"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..431
                     /locus_tag="WIB30_001971"
     CDS             <1..431
                     /locus_tag="WIB30_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ3797975.1"
                     /translation="KEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSRAGLDTQI
                     VLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRYGCTILRH
                     YRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            542..1924
                     /locus_tag="WIB30_001972"
     CDS             542..1924
                     /locus_tag="WIB30_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3797976.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYAGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            1928..2452
                     /locus_tag="WIB30_001973"
     CDS             1928..2452
                     /locus_tag="WIB30_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ3797977.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          542 a          717 c          800 g          602 t
ORIGIN      
        1 cgaaagaggg cgagcgttgg ttgtctgcca tgtcggcacg tttggcaagg ttcgtccccg
       61 aggaggagga gcggcgcagg ctgctggtca atatccagta cgaaagcagc cgggccggtt
      121 tggatacgca gattgtgttg gggctgattg aggtggaaag cgcgttccgc cagtatgcaa
      181 tcagcggtgt cggcgcgcgc ggcctgatgc aggttatgcc gttttggaaa aactacatcg
      241 gcaaaccggc gcacaacctg ttcgacatcc gcaccaacct gcgttacggc tgtaccatcc
      301 tgcgccatta ccggaatctt gaaaaaggca acatcgtccg cgcgcttgcc cgctttaacg
      361 gcagcttggg cagcaataaa tatccgaacg ccgttttggg cgcgtggcgc aaccgctggc
      421 agtggcgttg attttgaacc cgcgccgcaa ccgaaatacg gcgaatcctg tataatccga
      481 aaatctgttc actggaagtt cagacggcat tgcaactgtt gatgccgtct gaaaaaatat
      541 gatggcaaga gacaaccgca tccaaatgtt tccgcacgaa tggcgcgcca gtacgacgct
      601 ttccggcgtg tacgcgctgc gtatgctggg tatgttcctc gtgctgcccg tattggcggt
      661 gtatgccgcc tcgctgcccg gcgcggaagg caacaaaacg ctggtcgggc tggcaatggg
      721 catttacggg ctgacacagg ctctgctgca actgcctttg ggcatcgctt ccgacaagtt
      781 cgggcgcaag aaaaccattt atgcgggact ggtcgtgttt gcggcgggca gctttcttgc
      841 cgccgccgcc gatacgctgc ccatgctggt cgccgcacgc gccatacagg gtgcaggggc
      901 ggtcagtgcg gcggttaccg cgctgctggc ggatttgacg cgcgacggcg tacgtacccg
      961 cgcaatggcg atgatcggtt tgagtatcgg tttgacgttt tcggtcagcc tcgtcgttgc
     1021 ccccgtgatt gccgacgcgg tcggcgttcg cggactgttt atgctgaccg gcattctgac
     1081 cgtcatcagc atcggcgtgg tggcgtggat gactcccgat cccgaagttt ccaagctgca
     1141 cgaagatacg caggcgcagc cttcgcgcat aggcgaagtt ttgaaaaacc gtaggctgct
     1201 gacgcttgat ttcggcattt tcgccctgca cgccgcacaa atggcattgt ttaccgcgct
     1261 gcctttcgcg atgacccagc tcggtttgga aaaaatacag cattggaaag tctatctgcc
     1321 ttcgaccatt acgggcttgg tggtgatggt tccgctgatt atcgtcggcg agacgcgcaa
     1381 caagcttaag caggtttttg ttttgggtat cgtctgtatt gcggcggcgc agctcggttt
     1441 gctgtccggt atgcgctcgg taggcttgat taccgcttat ttggttgttt actttatcgg
     1501 ttttaatgtg ttggaagcga gcctgccgtc tatggtttcc aaaatcgcgc cgtccgacct
     1561 gaagggtacg gcgatgggcg tgtacaacac gatgcagtcg ctcggactgt ttgccggcgg
     1621 cgcggcaggc ggtttgctgt ttcaaaaata cggcttttcc ggcgtgtttg ccttttgcag
     1681 tatattgatg ctgctgtggc tggtaattgc cgttttatcg cctgcgccca agcccgtcaa
     1741 aaacctcagt taccctgtcg gcggcgtgtg gcagggcaat caggaagggt tataccgcgc
     1801 cttgtcggag cttgagggtg tggaagacat cggtttcagt ttcgacgggc agaccgtcta
     1861 tctcaaagtg ttgcagaagg gtttcgatca ggctgccgct gaaaaaatca tcacaggagt
     1921 ttaaaaaatg tcattgaaca aagtcatcct catcggccgc ctcggacgcg atcccgaagt
     1981 gcgctatatg cccaacggcg aggcggtttg taatttcagc gtcgccacca gcgaaacttg
     2041 gaacgaccgc aacggccaac gtgtagagcg tactgagtgg cacaacatca ccatgtaccg
     2101 caaactggcg gaaattgccg ggcaatacct caaaaaaggc gggctggttt atttggaagg
     2161 cagaatccaa agccgcaaat accaaggcaa agacggcatc gaacgcaccg cttacgatat
     2221 tgtcgccaac gaaatgaaaa tgttgggcgg gcgcaatgaa aacagcggcg gtgcgcctta
     2281 cgaggaaggt tacggtcaga gtcaggaggc ttaccaacgc cccgcgcagc aaagccggca
     2341 gcccgcctcc gacgcgccgt cccatcccca agaagcacca gccgcgccgc gccgccaacc
     2401 cgtgcctgcc gccgccccgg tcgaggacat tgacgacgat atcccgttct gaattttacg
     2461 gccggacatc ctcgcggagg gaaatcataa aggacggaga aaccttaaac cttacggggc
     2521 agggtttctc ttttttgcat ttgggccggc ttgttgccgc cctttgcgtt gggcggaacg
     2581 ttgcgattgc gccgttcggg cgttttgagg cagtgatgcc tgccgcacgt ccccgctgtt
     2641 ttcagacggt atagtggatt a
//
LOCUS       DAWHRA010000179         2628 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWHRA010000179 DAWHRA010000000
VERSION     DAWHRA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.179"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            121..735
                     /locus_tag="WIB30_001974"
     CDS             121..735
                     /locus_tag="WIB30_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ3797978.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNSANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVLG"
     gene            896..2557
                     /locus_tag="WIB30_001975"
     CDS             896..2557
                     /locus_tag="WIB30_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ3797979.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPDE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVIGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVLLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT          515 a          664 c          682 g          767 t
ORIGIN      
        1 aaggcgaggc aacgccgtac cggtttttgt taatccacta tattttgacg gggttgaagt
       61 tgacatctac aaggtttaat gtttgaatac attcaaaaat atatttgaat gagatgtttt
      121 atgattcaaa atgtcgttac ttcaataatc ctgtattctg ggacagccgt agacttactt
      181 attatcctaa tgttattttt tgccaaaaga aaaagcagaa aagacatcat taacatctat
      241 ttaggacaat ttctaggctc tgttagcctg atattgctaa gtttgctttt tgcatttgtc
      301 ttagattata ttcctagtaa agagatttta ggtttgctcg gcttgattcc aattttccta
      361 ggcatcaaag ttttgctgtt aggagattct gatggagagg ctattgccaa agagggtttg
      421 cgcaaagata ataaaaacct gatttttcta gtcgctatga ttacttttgc aagttgtggt
      481 gctgacaata ttggtgtctt tgtcccatat tttactacct taaattcagc gaatttgata
      541 gtggctttac ttacctttct agtcatgatt tatctcttgg ttttttctgc ccaaaaattg
      601 gcacaagtcc cttctgtcgg agaaactttg gaaaaatata gcagatggtt tgttgctgtt
      661 gtttatttag gattgggggt atatatcctg attgaaaaca acagttttga tatgctatgg
      721 actgtgttgg gctaggaaaa aatattatga aaaagatagt atctgcaaag actgccatgt
      781 gcagtctttt ttgttgccgg tctttttgtg tctgatgccg tctgaagcag tctctgcacg
      841 acctttgtgc gaatatttgc tacacttggc aatccgtcca ttttttctca cgcttatgct
      901 gacctatacc ccgcccgatg cccgcccgcc cgccaaaacc cacgaaaagc cgtggctgct
      961 gctgttgatg gcgtttgcct ggttgtggcc cggcgtgttt tcccacgatt tgtggaatcc
     1021 tgacgaacct gccgtctata ccgccgtcga agcactggca ggcagcccca cccccttggt
     1081 tgcccatctg ttcggtcaaa ccgatttcgg cataccgccc gtgtatcttt gggttgccgc
     1141 cgcgttcaaa catttgctgt cgccgtgggc tgccgactca tacgatgccg cacgctttgc
     1201 aggcgtattt tttgccgtta tcggactgac ttcctgcggc tttgccggtt tcaacttttt
     1261 gggcagacac cacgggcgca gcgtcgtcct gattctcatc ggctgtatcg ggctgattcc
     1321 agttgcccat ttcctcaacc ccgctgccgc cgcctttgcc gccgccggac tggtgctgca
     1381 cggttattct ttggctcgcc ggcgcgtgat tgccgcctct tttctgctcg gtacgggctg
     1441 gacgctgatg tcgttggcag cagcttatcc ggcagcattt gccctgatgc tgcccttgcc
     1501 cgtactgatg tttttccgtc cgtggcaaag caggcgtttg atgttgacgg cagtcgcctc
     1561 acttgccttt gccctgccgc ttatgaccgt ttacccgctg ctcttggcaa aaacgcagcc
     1621 cgcgctgttc gcgcaatggc tcgactatca cgttttcggt acgttcggcg gcgtgcggca
     1681 cgttcagacg gcattcagtt tgttttacta tctgaaaaac ctgctttggt ttgcattgcc
     1741 cgcgctgccg ctggcggttt ggacggtttg ccgcacgcgc ctgttttcga ccgactgggg
     1801 gattttgggc gtcgtctgga tgcttgccgt tttggtgctg cttgccgtca atccgcagcg
     1861 ttttcaggat aacctcgtct ggctgcttcc gccgcttgcc ctgttcggcg cggcgcaact
     1921 ggacagcctg aggcgcggcg cggcggcgtt tgtcaactgg ttcggcatta tggcgttcgg
     1981 actgtttgcc gtgttcctgt ggacgggctt tttcgccatg aattacggct ggcccgccaa
     2041 gcttgccgaa cgcgccgcct atttcagccc gtattatgtt cctgatatcg atcccattcc
     2101 gatggcggtt gccgtactgt tcacaccctt gtggctgtgg gcgattaccc ggaaaaacat
     2161 acgcggcagg caggcggtta ccaactgggc ggcaggcgtt accctgacct gggctttgct
     2221 gatgacgctg ttcctgccgt ggctggacgc ggcgaaaagc cacgcgccgg tcgtccggag
     2281 tatggaggca tcgctttccc cggaattgaa acgggagctt tcagacggca tcgagtgtat
     2341 cggcataggc ggcggcgacc tgcacacgcg gattgtttgg acgcagtacg gcacattgcc
     2401 gcaccgcgtc ggcgatgtac aatgccgcta ccgcatcgtc ctcctgcccc aaaatgcgga
     2461 tgcgccgcaa ggctggcaga cggtttggca gggtgcgcgt ccgcgcaaca aagacagtaa
     2521 gttcgcactg atacggaaaa tcggggaaaa tatataaaaa acaacagatt gagccgaatt
     2581 tctggattaa gtgccggaaa tcgcgtataa ttgcgcgatt aaaccttt
//
LOCUS       DAWHRA010000180         2566 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWHRA010000180 DAWHRA010000000
VERSION     DAWHRA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.180"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..2400
                     /gene="ileS"
                     /locus_tag="WIB30_001976"
     CDS             <1..2400
                     /gene="ileS"
                     /locus_tag="WIB30_001976"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ3797980.1"
                     /translation="VARQKKDFIRLGVLGDWDRPYLTMDFKTEADTVRMLGEIYKSGY
                     LYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAALAAAFGLAGFEGKA
                     FAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAEDALKRYGFSDGISI
                     LAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAPAHGLEDYAVCNKYG
                     IELYNPVNAEGKYIGETLRVAGMRVWEANPVILQWLEETGNLLASSKIEHSYAHCWRH
                     KTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWGRARLEAMIEGRPDW
                     VVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIEAWFSLDKSELLSAE
                     DCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSDQHRGWFQSSMLTGC
                     ASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFGADILRLWAASTDYS
                     GELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQADMVEIDRYALVLAR
                     RLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLYTTKADSHARRSAQT
                     ALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEFPTINEKAEAELVKK
                     WTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMASYLNALGEELRFALLVS
                     KAEVKVGDELAVAAKASDGEKCERCWHYTHDVGAVAGHETICKRCAGNVGGEGETRHY
                     A"
BASE COUNT          629 a          689 c          692 g          556 t
ORIGIN      
        1 gttgcccgcc agaaaaaaga ctttatccgc ttgggcgtgt tgggcgattg ggaccgtcct
       61 tacctgacca tggatttcaa aaccgaagcc gataccgtgc gtatgctcgg cgaaatctac
      121 aaatccggct atctctaccg gggtgcgaaa ccggttcaat tctgcttgga ctgcggttct
      181 tcgctggccg aagcggaagt ggaatataaa gacaaaatct cgcccgcgat tgacgttgcc
      241 tatccgttta aagacaccgc cgcgcttgcc gccgcattcg gtttggctgg tttcgaaggc
      301 aaagcgtttg ccgttatttg gacaaccacg ccttggactt tgcctgcgag ccaagctgtt
      361 tcagcgggtg cagacgtggt gtatcaatta atcgatacgc ccaaaggcaa attggtattg
      421 gccaaagatt tggcggaaga cgctttgaaa cgctacggct tttcagacgg catttctatt
      481 cttgccgaaa ccaccggcga caagctggaa aacctgcaca tgaaccatcc gttcctcgaa
      541 cgcgatattc ccatgcttaa cggcgaacac gttaccaccg atgccggtac cggcttggtg
      601 catactgcgc ccgcgcacgg tttggaagac tacgccgtct gcaacaaata cggcatcgag
      661 ctttacaatc ctgtcaacgc cgaaggcaaa tacatcggcg aaacgctgcg cgttgccggt
      721 atgcgcgttt gggaggcgaa ccccgtcatc ctgcaatggt tggaagaaac cggcaacctt
      781 ttggcaagca gtaaaatcga acacagctac gcccactgct ggcggcacaa aacgccgctg
      841 atttaccgcg cgaccggtca atggtttgtc ggtatggaca aagccggtgc cgacggcaaa
      901 accctgcgcg acaaagccat caaggccgtg gacgacaccg aattcttccc gtcttggggt
      961 cgcgcgcgtt tggaagccat gattgaaggt cgtcctgact gggtggtttc acgccaacgc
     1021 tattggggca cgccgatgac tttctttgtt cacaaagaaa cgggcgagct gcatccgaac
     1081 tctgccgaac ttttggaaaa agttgccctg aaaatcgaag aaaaaggcat cgaggcttgg
     1141 ttctccctcg ataaaagcga attattaagc gcggaagatt gcgaacatta cgacaaactc
     1201 cccgatacga tggacgtatg gttcgattcc ggttcgaccc attattccgt tgtgaaacaa
     1261 cgcgaagaat tggaatggcc ggcggatttg tatctcgaag gcagcgacca gcaccggggc
     1321 tggtttcaat cgtccatgct gaccggctgc gcctcatcaa tgggacgcgc accgtataaa
     1381 cagctgctga cccacggttt tgttgtagac ggcgaaggca aaaaaatgtc gaaatccatc
     1441 ggcaacgtcg tcgcgccgca agaagtctat aacgaattcg gcgcggacat cctgcgtctg
     1501 tgggcggcat ctaccgatta cagcggcgaa ttggcgattt ctaaagaaat ccttaagcgc
     1561 gtaaccgaaa gctaccgccg tatccgcaat accttgagct ttttgtttgc caacttgagc
     1621 gactttaatc cgattgaaga tgccgtgcaa caggcggata tggtggaaat cgaccgctat
     1681 gccctggtgt tggcgcgtcg gctgcaagag cgtctggcag gcgattacta tccgcgctac
     1741 gccttccact tcgccgtaaa agacattgtt tctttctgct cggaagactt gggcgcgttc
     1801 tacctcgaca tcctgaaaga ccgcctctat accaccaaag cagacagcca tgcacgccgc
     1861 agcgcacaaa ctgccctgta tcacatcaca cgcagtttgg ttctcttgat tgcaccgatt
     1921 ttgtgcttca ccggcgagga agcgtgggac atcatcggcg gcggcgaaga agacagcgtc
     1981 cttttccata cttggcacga gttcccgacc atcaacgaaa aagccgaagc cgaactggtg
     2041 aaaaaatgga cggcaatccg cgaagcccgc gaagcggtaa ccgccgccat tgagcctttg
     2101 cgcgccgaca aaaccgttgg ttcgtccttg caggcggaag ccgaaatcac tgcgccggaa
     2161 gaaatggcca gctacctgaa cgctttgggc gaagaattgc gctttgccct gctggtgtct
     2221 aaagccgaag tgaaagtagg tgatgaactt gccgttgccg ccaaagccag cgatggtgaa
     2281 aaatgcgaac gctgttggca ctacacccac gatgtgggcg cagttgcagg ccatgaaacc
     2341 atctgcaaac gctgcgccgg gaatgtcggc ggagaaggcg aaacgcgcca ttacgcctga
     2401 taaagtttga gcaaatgccg tctgaaaccg ccaaacagca tttcagacgg cattttttgt
     2461 accgcgattt gtctttataa cggcggaggg gtttcaagat tgcggtgttg tcggaatgca
     2521 actgaaccgt cattcccacg aaagtgggaa tctagaatct cgaggt
//
LOCUS       DAWHRA010000181         2484 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWHRA010000181 DAWHRA010000000
VERSION     DAWHRA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.181"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            1..577
                     /locus_tag="WIB30_001977"
                     /pseudo
     CDS             1..577
                     /locus_tag="WIB30_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
     gene            693..2201
                     /gene="ppx"
                     /locus_tag="WIB30_001978"
     CDS             693..2201
                     /gene="ppx"
                     /locus_tag="WIB30_001978"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ3797981.1"
                     /translation="MTTHPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
BASE COUNT          611 a          722 c          639 g          512 t
ORIGIN      
        1 gtgccgtttg tcgggcaaaa cctcttaggc ggcagccgat acaatttcgt cggcggcttt
       61 aatgccgatt tctcccaaca cttgagcgaa cgctggcggt tgacactaaa cgcgggcaat
      121 atgtggaagc attatcagga agaccgcacc gccgcccggt acgacagcca tatgccgctg
      181 gcgggcgcga cgctgatgta ttccgcgccg aaagactggc tgctttacgg cggggcggac
      241 tggtcgcacg acttgacgaa ggaggcggaa caggcttccg tccgcaaggg tttgcgtgtc
      301 ggcgcagtca aaacgttcga cggcgggctt gggtctgcgg gcaaacctgc gctatacccg
      361 caggatgttt gacgcacccg ggaccattgt gtaccgcttc ccgcgcaaag accacgaata
      421 tcaggcaaac ctgtcgttgt ggcacgacaa aatctcttgg aagggcttta cgccgcaact
      481 caatttccgc tatctgaaaa tcgacagcaa tatgaaaagt ttttacacac gcaaaaacac
      541 gcagattttc atgagcgtgg aaaaggattt caaataagcg caaaaatgcc gtcggcaaca
      601 tccgtgggca gaatcaaaaa ccgccgcatc atttattgtc aacgcctgcg ccgtcagagt
      661 aacattgcgt ttttccccca ccggtatcca ccatgaccac ccatcccgca aacgtcctcg
      721 cctccgtcga tttgggttcc aacagtttcc gcctccagat ttgcgaaaac aacaacggac
      781 aattaaaagt tatcgattcg ttcaaacaga tggtgcgctt cgccgccggt ctggacgaac
      841 agaaaaacct gagtgccgct tcccaagaac aggctttgga ctgcctggca aaattcggcg
      901 aacgcctgcg cggcttccgc cctgaacagg tgcgcgccgt ggcaaccaat acattccgcg
      961 tcgccaaaaa catcgcagat ttccttccca aggccgaagc ggcactgggt ttccccatcg
     1021 aaatcatcgc agggcgcgaa gaggcgcggc tgatttatac cggcgtgatc cacaccctcc
     1081 ccccgggcgg cggcaaaatg ctggttatcg acatcggggg cggttcgaca gaatttgtca
     1141 tcggctcgac gctgaatccc gacattaccg aaagcctgcc cttgggctgc gtaacctaca
     1201 gcctgcgctt cttccaaaac aaaatcaccg ccaaagactt ccaatctgcc atttccgccg
     1261 cccgcaacga aatccagcgc atcagcaaaa atatgaggcg cgaaggttgg gatttcgccg
     1321 tcggcacatc gggttcggca aaatccatcc gcgacgtgct tgccgccgaa atgccccaag
     1381 aggcggacat tacctacaaa ggcatgcgcg ccctcgccga acgcatcatc gaagccggtt
     1441 cggtcaaaaa ggccaaattt gaaaacctga aaccggaacg catcgaagtt tttgccggcg
     1501 gacttgccgt gatgatggcg gcgtttgaag aaatgaaact cgacaggatg accgtaaccg
     1561 aagccgccct gcgcgacggc gtgttttacg atttgatcgg gcgcggttta aacgaagata
     1621 tgcgcggaca aacggttgcc gagttccaac accgctacca cgtcagcctc aatcaggcga
     1681 aacgcaccgc cgagaccgcg caaaccttta tggacagcct ctgccacgct aaaaatgtta
     1741 cggttcaaga gcttgccttg tggcaacagt atctcggacg cgccgccgcg ctgcacgaaa
     1801 tcggtttgga catcgcccac accggctatc acaagcattc cgcctacatc ctcgaaaacg
     1861 ccgatatgcc gggtttctca cgcaaagaac agaccatact tgcccaactg gtcatcggtc
     1921 atcgcggcga tatgaaaaaa atgagcggca tcatcggcac caacgaaatg ttgtggtatg
     1981 ccgttttgtc gctgcgcctt gccgcactgt tctgccgttc gcgccaagac ctgtctttcc
     2041 cgaaaaatat gcagttgcgt acggacacgg aaagctgcgg cttcatcctg cgtattgaca
     2101 gggaatggct ggaacgccat cccctgattg ccgacgcatt ggaatatgaa agcgtccaat
     2161 ggcaaaaaat caatatgccg ttcaaagtcg aggccgtctg aaccttgcgg ggcaaatgcc
     2221 gtctgaatga tgttcagacg gcatttccct gtccgcaaca tcccgatatg cgcggcacat
     2281 ctgctcggaa cggtcatgca ggcgtaaaaa acaaggggca tacaactcaa aaaccgcctg
     2341 aaaatcttca ggcggtttcg tttgggttgc cggcaggcgg catcccatca tttttgccaa
     2401 ggcaacaaat tatttggcgg catctttcat tttgtctgcc gcttcctgag tcgcgtcggc
     2461 agctttgttc aaagtatctt tagc
//
LOCUS       DAWHRA010000182         2479 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWHRA010000182 DAWHRA010000000
VERSION     DAWHRA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.182"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(13..819)
                     /locus_tag="WIB30_001979"
     CDS             complement(13..819)
                     /locus_tag="WIB30_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ3797982.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKRELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="WIB30_001980"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="WIB30_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ3797983.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNNQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGIVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMRGIK
                     GMFPGL"
BASE COUNT          685 a          627 c          641 g          526 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttgc cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccgct
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcagact
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 gagcagcgaa gttccgatat gcagcataaa gggaaagtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 catcagcagt gccgcgccgt ggacggtcaa tgccgcgccc aaaaccggca attccctctt
      721 ccacgggtaa tcccgaccgc gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtctgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccgttt cgggcaagcc ttgacggatt tgccgatatg ctgtccggca cacaagccgc
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggccaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt cgtcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagtg gcgggcagcc tgacgccgga tcaggcattt
     1261 atcggcgtgg tcaacaaagc cctgaccgaa ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttg cgccgcccgc cgtcgtttta atggcaggtt tgcagggcgc gggcaagacg
     1381 acgaccgtcg gcaaactcgc ccgcctgctg aaaaacaatc agaagaaaaa ggttttggtc
     1441 gtatctgccg acgtttaccg ccctgccgcg attgaacaac tgcgcctgtt ggccgaacag
     1501 gtcggcgtgg actttttccc atccgatacc aaccaaaaac cggttgaaat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgttttga tggtcgatac cgccggtcgt
     1621 ctggcaatcg atgaagagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccggc atcgtgttga ccaagatgga cggcgattcg
     1801 cgcggcggtg cggcattgtc cgtacgccat gtaaccggca aaccgattaa atttatcggc
     1861 gtcggcgaaa aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cagccgtatc
     1921 ctcggcatgg gcgacgtatt gaccctgatt gaagacgtcc aaaaaggtat tgatgaagaa
     1981 gccgccgcca aaatggcgaa aaagctacaa aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccagcaaat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat atcgaaacaa atccccgaag gaacggctga aaaagcgatg
     2161 ggcaaagtgg aagccatcat caactcgatg actccaaaag aacgcgccaa ccctgccctg
     2221 ctcaaagcca gccgcaaacg ccgtattgca atgggtgcgg gcacaaccgt gcaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacagatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaggg ggataaaagg gatgttcccg
     2401 ggtttgtaag ccgatttaac agaaaacgcc gtctgaaaag tttcagacgg cgtttttgtt
     2461 ttatattctg atttatagt
//
LOCUS       DAWHRA010000183         2469 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWHRA010000183 DAWHRA010000000
VERSION     DAWHRA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.183"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..328
                     /locus_tag="WIB30_001981"
     CDS             <1..328
                     /locus_tag="WIB30_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ3797984.1"
                     /translation="IATTVPAQQAFLDAAELMQWSIETEGLGLNVISHKILGKDHAQV
                     EFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKACCGK
                     YLKPVA"
     gene            complement(547..1341)
                     /locus_tag="WIB30_001982"
     CDS             complement(547..1341)
                     /locus_tag="WIB30_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ3797985.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1445..2170
                     /locus_tag="WIB30_001983"
     CDS             1445..2170
                     /locus_tag="WIB30_001983"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ3797986.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISLGDRMDFTLQKSVELGIT
                     AIQPIISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYRKALDKM
                     PSESTKLIMSINRACKLADIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          671 a          783 c          541 g          474 t
ORIGIN      
        1 catcgctact accgttccgg cgcaacaggc attcctcgat gccgccgaac tgatgcaatg
       61 gagtatagaa accgaagggc tgggcttgaa cgtcatctcg cacaagatac tcggcaaaga
      121 ccacgcccaa gtcgaatttg aagcctactt ccgagacgga caacaccgat ccgcgcatca
      181 cgaactgtcc ggcttcgtca acatcggcgg acaatggtat tttatcgatc ccaccgttcc
      241 gcatcctgcg atgaaacaac cctgcatttg cggatcaggc aaaaaattca aagcctgctg
      301 cggcaaatat ctgaaacctg tcgcataaaa atgccgtctg aacgttcaga cggcattttc
      361 aacgtgcaaa aaaaaaccat tcataccaag ggtaagtatg aatggtcaat acattgcggg
      421 aaaacgtctt acttgctgca ctgccgaaaa gggagaaacg gcagcggtaa tcagcggaaa
      481 ggattgtacc cgaattaata ttaagaaacg ttaatcgcga aaatatatta acaaacctgt
      541 tgaaacctat tggttttccc gtatccaccc gacccagcgt tcaaacagct tcggttcgag
      601 cgcggcaacg accgagcgtt tgaacacgtg ttcaccactc caaaacccgt cgccttccaa
      661 agtcgtcagc ctgccgcccg cctcctcgaa aatcaacgcg ccggcggcat aatcccacag
      721 cttctgcccg ccgtgaacat aaacatcata acgcccgcac gccagataac accaatccaa
      781 cgtactgctg cccatactcc gtatcgttcc aaaaggcgcg agcgtactca tacggctgga
      841 aagtttgccc gaacgcagat atttgatttc cacgcccgca atcgcctcat tgagtttttt
      901 atccacgagg cgcaggggca gacgcgtccc gtttaaaaac gccccctgcc cgcgttcggc
      961 ataaaaacat tcgccgctga ctgggttgta gattacgccc aactcggcgc gcccgttgcg
     1021 gacaaacgcc accgataccg caaaatgcgg cagcccgttg acaaaattgt tcgtcccgtc
     1081 tatcggatcg acaatccaca gccccttttc ccccgaatat tgttcccaca aagccgactg
     1141 ttcctgccgc gacatttcct cacccaacat cggactgtcg attaaaagcg gcaacgcggc
     1201 ggcaaaagcc gtctgcgcgg caatgtccgc ctcgctcaac atcgaaccgt cttccttgcg
     1261 gtgagacggc gtattcaaaa aacgcggcat aatttcggtt tgcgcgatat ggcgcacgac
     1321 tttctgcaaa cggtgtaaca cttcctactg tcctcatatt ttgaacttgc ggcgcgcgaa
     1381 cgtataatgt ccgcttccat cacgccgctg cgacggatta taaccgtccg aaccgccaaa
     1441 aactatgccc cgattccacc tgcccgaaaa cctttccgtc ggacaaaccg tcgccctgcc
     1501 cgacaacatc gtccgccacc tcaacgtcct gcgcgtccgc cccaacgaaa acatcaccct
     1561 cttcgacggc aaaggcaagg cacacgccgc acggctgacc gttttggaaa aacgccgcgc
     1621 cgaagccgaa atcctgcacg aagacacaac cgacaacgag tccccgctca acatcacgct
     1681 catccaatcc atttccttag gcgatcgcat ggatttcacc ctgcaaaaaa gcgtcgaact
     1741 cggcataacc gccatacagc ccatcatcag cgaacgctgc atcgtccgcc tcgacggaga
     1801 acgcgccgcc aaacgcctcg cacgctggca ggaaatcgtc atctccgcgt gcgaacagag
     1861 cggcaggaac accgttcccc ccgtactgcc tatcatcggc taccgtaaag cactcgacaa
     1921 aatgccgtct gaaagcacca agctgattat gagcatcaac cgcgcctgca aacttgccga
     1981 catccgccac ccgtccggcg caatcgtctt tatggtcggc cccgaaggcg gctggacaga
     2041 acaggaagaa caacaggcat ttgaagctgg ctttcaggcg gttacactcg gcaaacggat
     2101 tttacgcaca gaaaccgccc cactcgccgc cctcgccgcc atgcagacgc tttggggcga
     2161 tttcgcataa acagaaatgc cgtctgaaac ccgttcagac ggcattttgc agccgattaa
     2221 gatagtaggt tcaaataaga tttcccgtgt cgtcattccc gcgaaagcgg gaatctagaa
     2281 acgaaaaact acagagattt atccgaaaca acaaccctct ccgccgtcat tcccgcaaaa
     2341 gcgggaatct agaaacgaaa aactacaggg atttatccga aacaacaaac cctctccgcc
     2401 gtcattcccg cgcaggcggg aatctagaaa cgaaaaacta cagggattta tccgaaacaa
     2461 caaaccctc
//
LOCUS       DAWHRA010000184         2443 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWHRA010000184 DAWHRA010000000
VERSION     DAWHRA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.184"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            374..2170
                     /locus_tag="WIB30_001984"
                     /pseudo
     CDS             374..2170
                     /locus_tag="WIB30_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
     gene            2325..>2443
                     /locus_tag="WIB30_001985"
     CDS             2325..>2443
                     /locus_tag="WIB30_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ3797987.1"
                     /translation="MIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTRYLRDHRG"
BASE COUNT          744 a          602 c          591 g          506 t
ORIGIN      
        1 ataaaacagt ttccttagat tccacgtcct agattcccgc tttcgcggga atgacgagat
       61 tttagattat gggaatttat caggaatgat tgaatccata gaaaaaccac aggaatctat
      121 cagaaaaaac agaaacccca ccgccgtcat tcccgcaaaa gcgggaatcc agacccgtcg
      181 gcacggaaac ttaccgaata aaacagtttc cttagattcc acgtcccaga ttcccgcctt
      241 cgcgggaatg acgagatttt aagttggggg aatttatcag aaaaccccca acccccaaaa
      301 accgggcgga tgccgcacca tccgccccca aaccccgatt taaccattca aacaaaccaa
      361 aagaaaaaac aaaatgaaca aaatataccg catcatttgg aatagtgccc tcaatgcctg
      421 ggtcgccgta tccgagctca cacgcaacca caccaaacgc gcctccgcaa ccgtggcgac
      481 cgccgtattg gcgacactgt tgtttgcaac ggttcaggcg agtactaccg atgacgacga
      541 tttatattta gaacccgtac aacgcactgc tcccgtgttg agcttccatg ccgattcaga
      601 aggcacggga gaaaaagaag ttataggaaa tacaaatttg ggaatatatt tcgacgagaa
      661 aagagtacta aaagccggaa caatcaccct caaagccggc gacaacctga aaatcaaaca
      721 aaacaccgat gaaaacaccg atgaaaacac caatgccagt agcttcacct actcgctgaa
      781 aaaagacctc acagatctga ccagtgttga aactgaaaaa ttatcgtttg gcgcaaacgg
      841 caagaaagtc aacatcacaa gcgacaccaa aggcttgaat ttcgcgaaag aaacggctgg
      901 gacgaacggc gacaccacgg ttcatctgaa cggtatcggt tcgactttga ccgatacgct
      961 tgcgggttct tctgcttctc acgttgatgc gggtaaccaa agtacacatt acactcgtgc
     1021 agcaagtatt aaggatgtgt tgaatgcggg ttggaatatt aagggtgtta aaactggctc
     1081 aacaactggt caatcagaaa atgtcgattt cgtccgcact tacgacacag tcgagttctt
     1141 gagcgcagat acgaaaacaa cgactgttaa tgtggaaagc aaagacaacg gcaagagaac
     1201 cgaagttaaa atcggtgcga agacttctgt tattaaagaa aaagacggta agttggttac
     1261 tggtaaaggc aaaggcgaga atggttcttc tacagacgaa agcgaaggct tagtgactgc
     1321 aaaagaagtg attgatgcag taaacaaggc tggttggaga atgaaaacaa caaccgctaa
     1381 tggtcaaaca ggtcaagctg acaagtttga aaccgttaca tcaggcacaa atgtaacctt
     1441 tgctagtggt aaaggtacaa ctgcgactgt aagtaaagat gatcaaggca acatcactgt
     1501 tatgtatgat gtaaatgtcg gcgatgccct aaacgtcaat cagctgcaaa acagcggttg
     1561 gaatttggat tccaaagcgg ttgcaggttc ttcgggcaaa gtcatcagcg gcaatgtttc
     1621 gccgagcaag ggaaagatgg atgaaaccgt caacattaat gccggcaaca acatcgagat
     1681 tacccgcaac ggtaaaaata tcgacatcgc cacttcgatg accccgcagt tttccagcgt
     1741 ttcgctcggc gcgggggcgg atgcgcccac tttgagcgtg gatgacaagg gcgcgttgaa
     1801 tgtcggcagc aaggatgcca acaaacccgt ccgcattacc aatgtcgccc cgggcgttaa
     1861 agagggggat gttacaaacg tcgcacaact taaaggcgtg gcgcaaaact tgaacaaccg
     1921 catcgacaat gtggacggca acgcgcgtgc gggcatcgcc caagcgattg caaccgcagg
     1981 tctggttcag gcgtatctgc ccggcaagag tatgatggcg atcggcggcg gcacttatcg
     2041 cggcgaagcc ggttacgcca tcggctactc cagtatttcc gacggcggaa attgaattat
     2101 caaaggcacg gcttccggca attcgcgcgg tcatttcggt gcttccgcat ctgtcggtta
     2161 tcagtggtaa gggctttttc gcctgtctgc tgtttgggat aggcggaagg tttgaaggga
     2221 agggcggcga tttgccgcct gagacctttg caaaattccc caaaatcccc taaattccca
     2281 ccaagacatt taggagcacc ttcttccagc aaaccgccca agccatgatt gccaaacaca
     2341 tcgaccgttt cccgctattg aagttggacc aggtgattga ttggcagccg atcgaacaat
     2401 acctgaaccg tcaaagaacc cgttacctcc gagatcaccg cgg
//
LOCUS       DAWHRA010000185         2433 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWHRA010000185 DAWHRA010000000
VERSION     DAWHRA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.185"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            35..1390
                     /locus_tag="WIB30_001986"
     CDS             35..1390
                     /locus_tag="WIB30_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ3797988.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKSY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WIB30_001987"
     CDS             1394..2404
                     /locus_tag="WIB30_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ3797989.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     TYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          436 a          668 c          740 g          589 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcagatg agccgcatct tccagacgct
      181 cggtgccgat ccgcacagcc tcggctggtt ctttatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcgaa tatggcgatg cagccgttta agatgatggt
      481 cggcgatatg gtcaacgagg agcagaaaag ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg ttcgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccact gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat ttcggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttacctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaacggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgcat tggcccgagt tcggcaatat
     2341 ccgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg acc
//
LOCUS       DAWHRA010000186         2425 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWHRA010000186 DAWHRA010000000
VERSION     DAWHRA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.186"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(148..735)
                     /locus_tag="WIB30_001988"
     CDS             complement(148..735)
                     /locus_tag="WIB30_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ3797990.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFSACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            898..2304
                     /gene="dnaB"
                     /locus_tag="WIB30_001989"
     CDS             898..2304
                     /gene="dnaB"
                     /locus_tag="WIB30_001989"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ3797991.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAFNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          618 a          620 c          637 g          550 t
ORIGIN      
        1 cgaaacctga caataaccgt agcaactaaa ccgtcattcc cacgaaagtg ggaatctaga
       61 aataaaaagc agcaggaatt tatcggaaat aactgaaatt caataccgca aaaatctacc
      121 cgaaatgata tagcggatta acaaaaatca ggacaaggcg gcgaagcgtt tggcgacttc
      181 gtcccagttg acgatttccc aaaaaccttt caggtagttg gggcggctgt tgcggtagtc
      241 gatgtagtag gcgtgttccc acacgtcgca ggtcagcagc ggcgtgtttt cagtggtcag
      301 cggcgtagcg gcgttggaag tggaaaccaa atccaatccg ccggcagggg ttttcaccag
      361 ccacgcccaa ccggagccga aagtaccggc agcgcaagcg gagaatgctt cttggaattt
      421 ctcgaagctg ccccatttcg cgtcgatggc ggcggccagt tcgccggaag gtttgccttg
      481 acctttgggc gtgaaaccca gccagtagaa agtgtggttc caagtttggg ctgcgttgtt
      541 gaacacgccg cccgaagatt ttttcacaat ctcttccaaa ggcaggtttt caaattcggt
      601 gcctttgatt tgattgttca ggttggtgat gtaggtttga tggtgtttgc cgtagtggaa
      661 ttccaaagtt tctttgctca gatgcgggga caatgcgtcc agttcataag gcagttgcgg
      721 cagcttatgt tccattttgt gctcctaata ttgttttaaa tgttgtattt tggcagtgtt
      781 gctgcaaata gctcagcagc gcacatattc tacctgtttt gaggtacgga aaccaattaa
      841 acctgcttta cgctataata gaagattgca atttcggcac gacagatagg atgtaccatg
      901 aacgattaca cagctatgcc gtctgaagac cgtgaggtcg gcgcattgtc gctgcctcca
      961 cattcgatgg aggcggaaca atccgttttg ggcgggttga tgctggaaaa tccggcctgg
     1021 gacaggattg ccgatgtggt ttcgggagag gatttctacc gccacgaaca ccgcctgatt
     1081 ttccgatcca ttgccaaatt aatcaatgag agccgtcccg ccgatgtgat tacggttcag
     1141 gaagatttgc agcggaacga agaattggaa gcggcaggcg gattcgaata cctgattacg
     1201 ctggcgcaaa acaccccgtc tgccgccaac atccgccgct atgccgaaat cgtgcgcgag
     1261 cgttccatta tgcgccaact cgccgaagtg gggacggaaa tcgcccgcag cgcattcaat
     1321 ccgcaaggca gggacgcggg gcagcttttg gacgaggcgg aaaacaaagt attccaaatc
     1381 gccgaaagca cggccaaatc caagcagggc tttttggaga tgcccgattt gctgaaagaa
     1441 gtcgtacagc gcatcgatat gctctactcg cgcgacaatc ccgatgaagt taccggcgtg
     1501 ccgacagggt ttatcgacct cgacaaaaaa acctcgggcc ttcagcccgg cgacctgatt
     1561 attgttgccg gtcgtccgtc tatgggtaag accgcctttt ccatcaatat cgccgaacac
     1621 gttgccgtag aaggcaagct gccggttgcc gtcttctcaa tggagatggg cggggcgcaa
     1681 ctggtcatgc gtatgctcgg ctcggtcgga cgcttggatc aaagcgtttt gaaaaccggc
     1741 aggctcgaag acgaacactg gggtcgcctg aacgaagcag tcgtcaaact ctccgacgcg
     1801 cccgtgtaca tcgacgagac cccgggtctg accgcgctcg aactgcgcgc ccgtgcccgc
     1861 cgtctcgccc gtcaatttaa caataagctg ggattaatcg tcatcgacta cctgcaactg
     1921 atggcaggat ccggccgttc cgacaaccga gcttcagagc tgggcgaaat ttcacgttcg
     1981 ctcaaagcgt tggcgaaaga attgcaagtc cccatcatcg ccctgtcgca attgagccgt
     2041 accgtcgaat cgcgtaccga caaacgcccg atgatgtccg acctccgcga atccggcgca
     2101 atcgagcaag atgccgacct gattatgttc atgtaccgcg acgaatacta caaccaggac
     2161 tcacccatga aaggccttgc cgaatgtatc atcggcaaac accgcaacgg tcccgtcggt
     2221 aaaatcttcc tcacatggac gggacaattc accaaattcg acaatgctgc ctatattccc
     2281 gaggaggcaa agatagaaga ttaaatggct atataaaaat ttattaggcg aaatcaggca
     2341 aaatcgttta aaatcatgct gagagattgc cctaaaaaat aaaacgcggt cttgaggcat
     2401 ttttgcattc agcccgcata taatt
//
LOCUS       DAWHRA010000187         2399 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWHRA010000187 DAWHRA010000000
VERSION     DAWHRA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.187"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            79..2292
                     /locus_tag="WIB30_001990"
     CDS             79..2292
                     /locus_tag="WIB30_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ3797992.1"
                     /translation="MTAISPIQDTQSATPQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QENYPADAATPYGEPLSDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          627 a          768 c          576 g          428 t
ORIGIN      
        1 attttagtaa cctatgttat tgcaaaggtc tcaagctgtt acaataaact cgtttttatc
       61 ggaacggaag accccatcat gaccgccatc agcccgattc aagacacgca aagcgcgacc
      121 ccgcaagaat tgcgcgaatg gttcgacagc tactgcgccg cgctgccgga caacgataaa
      181 aacctcatcg gtaccgcatg gtcgctggcg caggaaaact atcccgccga tgccgccacg
      241 ccgtatggcg agccgttgtc cgaccacttc ctcggtgcgg cgcaaatggt ccatgaactc
      301 gacctgctgc ccgatgccgt cgccgccacc ctgcttgccg acatcggacg ctacgtcccc
      361 gactggaacc tattggtttc cgaacgctgc aacagtaccg tcgccgagct ggtcaaaggt
      421 gtggacgaag tgcagaaact cacccacttc gcccgggtgg acagcctcgc cacgccagaa
      481 gaacgcgccc agcaggcaga aactatgcgg aaaatgctgc tggcgatggt taccgacatc
      541 cgcgtcgtgt taatcaaact ggcgatgcgt acgcgcaccc tgcaattttt aagcaacgcc
      601 cccgacagcc ccgaaaaacg cgccgtcgcc aaagaaaccc tcgacatctt cgccccgctc
      661 gccaaccgtt tgggcgtgtg gcagctcaaa tggcagctcg aagatttggg cttccgccat
      721 caagagcccg aaaaataccg cgaaattgcc ctgcttttgg acgaaaaacg taccgaacgc
      781 ctcgaataca tcgaaaactt ccttaatatc ctgcgtacgg aactcaaaaa atacaatatc
      841 cactttgaag tcgccggccg tccgaaacac atctactcca tttacaaaaa aatggtgaag
      901 aaaaaactca gcttcgacgg gttgttcgac atccgcgccg tgcggattct ggttgatacc
      961 gtccccgagt gttacaccac actgggcatt gtccacagcc tctggcagcc cattcccggc
     1021 gagttcgacg actacatcgc caacccgaaa ggcaacggct ataaaagttt gcacaccgtc
     1081 atcgtcggcc cggaagacaa aggcgtggaa gtgcaaatcc gcaccttcga tatgcaccaa
     1141 ttcaacgaat tcggtgtcgc cgcgcactgg cgttacaaag agggcggcaa aggcgattcc
     1201 gcctacgaac aaaaaatcgc ctggttacgc caacttttgg actggcgcga aaacatggcg
     1261 gaaagcggca aggaagacct cgccgccgcc ttcaaaaccg agcttttcaa cgacacgatt
     1321 tatgttttga ccccgcacgg caaagtcctc tccctgccca caggcgcgac ccccatcgac
     1381 ttcgcctacg ccctgcacag cagcatcggc gaccgttgcc gcggtgcgaa agtcgaaggg
     1441 cagattgtgc cgctgtccac cccgctcgaa aacggacagc gtgtcgaaat cattaccgcc
     1501 aaagaagggc atccttccgt caactggctt tacgaaggct gggtcaaatc caacaaggca
     1561 atcggcaaaa tccgcgccta catccgccag caaaacgccg acaccgtgcg cgaagaaggc
     1621 cgcgtccaac tcgacaaaca gcttgccaaa ctcacgccca aacccaacct gcaagagctt
     1681 gccgaaaatc tcggctacaa aaagccagaa gacctctaca ccgccgtcgg acaaggcgaa
     1741 atttccaacc gcgccatcca aaaagcctgc ggcacgctga acgaaccgcc gcccgtaccc
     1801 gtcagcgaaa ccaccatcgt caaacagtcc aaaatcaaaa aaggcggcaa aaacggcgtg
     1861 ctcatcgacg gcgaagacgg tctgatgacc acgcttgcca aatgctgcaa acccgcgccg
     1921 cccgacgaca ttgtcggctt cgttacccgc gatcgcggca tttcggtaca ccgcaaaacc
     1981 tgcccctctt tccgacacct cgccgaacac gcgcccgaaa aagtactgga cgcaagttgg
     2041 gcggcgttgc aggaaggaca agtgttcgcc gtcgatatcg aaatccgcgc ccaagaccgc
     2101 tccgggcttt tgcgcgacgt atccgacgcg ctcgcccgcc acaaactcaa cgttaccgcc
     2161 gtgcaaaccc agtcccgcga cttggaagcc agtatgcggt tcacgctcga agtcaaacag
     2221 gtcaacgacc tcccgcgcgt cctcgccagc ctcggcgacg tcaaaggcgt attgagcgtt
     2281 acccggcttt aaatacaaaa atgccgtctg aaagccgaat aacgcttcag acggcatttt
     2341 gattgccggg gtttgctatt ttttgttgca tagtcaatta aaaacaaaat agtacaata
//
LOCUS       DAWHRA010000188         2380 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWHRA010000188 DAWHRA010000000
VERSION     DAWHRA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.188"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(120..1529)
                     /gene="dacB"
                     /locus_tag="WIB30_001991"
     CDS             complement(120..1529)
                     /gene="dacB"
                     /locus_tag="WIB30_001991"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ3797993.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTINDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLQITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMVVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1694..2260
                     /locus_tag="WIB30_001992"
     CDS             1694..2260
                     /locus_tag="WIB30_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ3797994.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          554 a          574 c          674 g          578 t
ORIGIN      
        1 gacgttttaa caacagtgag ataaaagttc ttgttccatt ttaaaacaat tagtaaaatc
       61 aagtttgtcc tagttatcta ggacagcccc ttaaatttaa tccactatat tttcctgttt
      121 caggctcggc gttctttgca catcagtttc gcatccagcc agccatcgcc gccggagatg
      181 atgttgttgg caacgaagtt gtccaagtcg ggcagcaggg aaacggcgcg accgctgttg
      241 atgatgacga ccaccaccat cggtttgtcg cccagccagt agcctgccaa tgcgcggaca
      301 ttgttgagcg tgccggtttt caggcgcaga agcccaccgc tttgtttgaa gcggttgcgt
      361 aaagttccgt ctgtgccggc gatgggcagc gtgtcgatga aatcttgtgc aaacgggctg
      421 aaataagccg tttccaacat ttgcgccatc attctcgccg ttaccctttc ttttctagac
      481 agacccgaac cgttttccaa aaccaaatcc gcaacatcga tgcccgacac ggcaagttcg
      541 cgccggacgg cagacgctgc ctgttcggaa acggcgggca gtttgccgtc gccgccgagt
      601 ttgaggaaga cggaacgcgc aattagattg tccgaacgct tgttcatgtc cgtcaaaatt
      661 tccttcatcg gtttggcgtg tgcaacggca agcgtctgcg cgccttccgg cgtgtcggct
      721 atgccgatac cgtctgaaat ccgtccgccg ccgagcagcc agtggttggt aaaactttgc
      781 cggatcagtt cgtcaagcgc gaacatccgg acaccgacag gcttgcccaa acagctctcg
      841 ggaatattgc cgcgcaattt cagcgtattg tccgaaaaag atgcacgcat cagttttttg
      901 atcgaagggc aggcagcttg ggaggcggta atttgcaagt tgttttgggc gaaaatatgc
      961 ggcaaaggcg gatcggtgag gatgtcggta ctgtcggcgg cattgcgttc ggcgcgcacc
     1021 ataaccatac cggcagacag catagttgga ttggggggcg tcataaacgg cgaaccgctg
     1081 tcggcttcga aatcgtcggg gctgccgact tcgccccaca ggctgtggtc gagcatcagg
     1141 tgtcccgtga tattgcgtat gccttgttcg cgcaactggc gttggacggc aagcaggttt
     1201 tcctgattga aaacggggtc gccgctgccc gcccaataca ggtttccgtc aagcgtgccg
     1261 tcgtttatcg taccgttgct tttaaactcg gtcgcccagc ggtaattgct gccgaaggtt
     1321 ttgaaggcgg caaacgcggt aacgagtttc attgtggagg cggggttgac ggggacatcc
     1381 gagcggtggt caatgatgac ttttccgctg tcaagctctt ggacatatac ggcgatttcg
     1441 ttttgcggaa tgcgacccgt atcgagcgcg tgtgcggcga gggaggcgag aagcagcagc
     1501 agggaggccg ctgttttggg gaaattcata ggtaaatctg ttacataaaa aaagcaatta
     1561 taaggcaaag ccggataagt gggaacgctg tggcggcggg ccggcttgtt tgcaggggaa
     1621 atcacatata taataatcgc tatcattatg aaatgattga gacgcacaaa cttaatattc
     1681 aggaggaatg attatggcta agaaaatcag tattttggtg ggcagcctgc gccgtgcttc
     1741 ttttgcgcgc aaagtggcat tgaatgcggc ggagatgttc cccgaaggct ggcaggcgga
     1801 aatcgtcgaa atcggacatc tgccgcttta caatttcgat tatgacgacc ctgcggtgga
     1861 agatgtgccg ctgcccgaaa gctacacggt tttccgcgaa acgattaagg cttcggacgg
     1921 cattttgttc gttacgtccg aaaataaccg caccattccc gcctgtttga aaaatgcggt
     1981 ggacatcggc tcgaaaccga atgccgacgt ggcgtggaaa aacaaaccgg ccggcatcat
     2041 cagccattcc gtcggcaaga tgggcggtta cagttcgcaa aaaaacctgc gccttgcgct
     2101 gtcttatttc gatatgcccg taaccggaca gccggaagtg tttttgggca attcgccgac
     2161 actgtttgat gaaaacggca aattgatcga ctcggcaagg gattttgttc agtcatatat
     2221 taatcagttt gtcggtttga ttgaaagaaa tgccaaataa acaaacaagt cgaaaggccg
     2281 caaaaccggt tcgggttttg cggctttttt atatgggctg ggcagcgtca aggtttgccg
     2341 tttgccggat aatgcgcggc aaatgaccgg cgtgtttata
//
LOCUS       DAWHRA010000189         2372 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWHRA010000189 DAWHRA010000000
VERSION     DAWHRA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.189"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..296
                     /locus_tag="WIB30_001993"
     CDS             <1..296
                     /locus_tag="WIB30_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ3797995.1"
                     /translation="LAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVG
                     LKHPLHVPRPPHWGGYIVIPDLLEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(426..1436)
                     /locus_tag="WIB30_001994"
     CDS             complement(426..1436)
                     /locus_tag="WIB30_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ3797996.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLTAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDININIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANLPDQEGILE
                     LHIRRHENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSNRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRNGHVQDIAAQDHPDLSEYEVFACGSPAMTEQTKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            1450..1569
                     /locus_tag="WIB30_001995"
                     /pseudo
     CDS             1450..1569
                     /locus_tag="WIB30_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1648..>2372)
                     /gene="gatB"
                     /locus_tag="WIB30_001996"
     CDS             complement(1648..>2372)
                     /gene="gatB"
                     /locus_tag="WIB30_001996"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEZ3797997.1"
                     /translation="LEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPDLLPV
                     IISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAKESGQ
                     GKLTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAFEAMW
                     AEPEATIAEIIEKHGLQQMTDTGEIEAMVDEVLANNAKAVEQFKSGNEKALNAIVGQV
                     MKASKGKANPAQVQELIKAKLA"
BASE COUNT          459 a          565 c          717 g          631 t
ORIGIN      
        1 ggctggcgga gaagttgtcg gacgaatatt ttgagagtcg cccgtatcag agccgtttgg
       61 gtgcgtgggc aagtgcgcag agcgaggtta ttccgaacaa ggcggtattg gtggcaaagg
      121 cggcggcggt tggactcaaa catcctctgc atgtgccgcg tccgccgcat tggggcggct
      181 atattgtgat tcccgatttg cttgagttct ggcagggcag accgagcagg ctgcacgacc
      241 gcatccaata ccgtttgctg gatggtggct ggatacgcga gcggctgtcg ccttgaacag
      301 gaaaaaacat atgccgtctg aaaaccggaa tcgggtttca gacggcattt tttcacgggg
      361 aaaacacgga cgggaagttt tttgtgttct gaacggaaag ctcgaatcct ctttataccg
      421 gggaattatg atgcggacgg cgtgaatgcg tcggagaaaa acaagttttc cggcagctta
      481 tgctgttgca caaacagatt ctttgtttgt tcggtcatgg ccggagaacc gcaggcaaat
      541 acttcgtatt ccgacaggtc ggggtggtct tgtgccgcga tgtcttgtac gtgaccattt
      601 cttccctgcc agccctctcc ggggcgggac aatacggggg tgaagcaggc gtttttcaga
      661 cggcatgcca acccttgtgc ttcttcgagg gcatacaaat catcctgatg acgcgcgccc
      721 cagtagaaat ggacggcgcg gttgctgcct tggcggataa ggtcgagcag gatgctgcgg
      781 atgggggcgt agcctgtgcc ggttgccagc aggatgacgg gtttgccgct gtcttcctgc
      841 aaggtaaacg aaccgagcgg gcctttaacg cggacgatgc ctttttcttt gactttgggt
      901 tcgctgccga aaatcatttc cgagcagaca ccgttttcgt gcctgcggat gtgcagttcc
      961 aaaatgcctt cttggtcggg taaattggcg atggagtagc tgcggctgac gttgcccggc
     1021 agcagtaaat caatgtattg cccggcgtag aaggcaaacg gcggggcttt gggcagggca
     1081 agtttcagga gggcgacatc gtgtttgaaa ataatacttt cgatgcgtgc gggcagggtg
     1141 cggacgggta gggcatcggc tttgtagccg gggatgttga tgttgatatc gctttgcgca
     1201 gtggtgcagc acatcaaaat cttgccttgc gctttttctg cttcggataa agcctgttcc
     1261 gagtgtccgc ccatttgaat atcgccgctg accagttcgg ctttgcattg tccgcagaca
     1321 ccgcttttgc aggaatgggg caggttgagg ttttgacggg cggcagcggt caaaacggtt
     1381 tcgccgtcgt tggcggcaaa ggtggtttgg tcgggcaggg taacggtgtg gttcatgtct
     1441 tggtatggtt tgagcagatt aagttatagt ggattaaatt taaatcagga caaggcgacg
     1501 aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa cgccgtactg gtttaaattt
     1561 aatccactat aaaaggccgt ctgaaagctt atttcctgtt ttaccttcgt tggaactttg
     1621 ctttcagacg acctgtgtga taacggatta agccagtttg gctttaatca gctcttgaac
     1681 ctgcgcgggg ttggctttgc ctttgctggc cttcatcact tgtcccacaa tcgcattcag
     1741 ggctttttcg ttgccggatt taaactgttc cacggctttg gcgttgtttg ccagcacttc
     1801 gtccaccatg gcttcaatct cgccggtgtc ggtcatctgt tgcaaaccgt gtttttcaat
     1861 gatttcggca atggtggctt cgggttctgc ccacatggct tcaaaggctt ttttcgctaa
     1921 cttgctgctt aatgtgccgt cggcgatttt gccaaccagc gcggcgaggc gcggggcggt
     1981 aatcgggctg tcggcaagtt ccatgccttc tttgttcagc gcggcggcaa gttcgccgtt
     2041 catccagttg gcagtcagct tgccttgtcc gctttctttg gcggcttctt caaaataggc
     2101 agcctgcgca cggcttgcgg tcagcaggcg cgcgtcgtat tcggacacgc cgtaatccgc
     2161 cacgaaacgc gctgccattt ctttcggcag ctcgggcatt tctgctttgg ctttttgcat
     2221 ttgggcgtct gaaatgataa cgggcagcaa atcagggtcg gggaagtagc ggtagtcgtg
     2281 cgcatcttct ttcaggcgca ttacgcgggt ttcgcctttt tcgggatcaa acagcatggt
     2341 tgcctgctgt actttgccgc cgtcttccaa aa
//
LOCUS       DAWHRA010000190         2352 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWHRA010000190 DAWHRA010000000
VERSION     DAWHRA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.190"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            125..2005
                     /locus_tag="WIB30_001997"
     CDS             125..2005
                     /locus_tag="WIB30_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ3797998.1"
                     /translation="MNIFYEESGQFKIASIVQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAVKPGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPAVSIYFDVDGE
                     FNVGEPTCKIEAVNIAENLRIQAIEPHFNAETGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPAGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          613 a          709 c          572 g          458 t
ORIGIN      
        1 tgttaatcca ctataacgca agcaccgcaa agacgcgcca accctctccc aacctttttt
       61 cagacggcat tttcggtaat ctgctaaaat cgcccgcttg agtttccaca gaaaaatccg
      121 aaaaatgaat attttttacg aagagtccgg ccaattcaaa atcgcctcta ttgtccaaaa
      181 aaacgatgcc acctaccaag tcgatacccc acacggcaaa cgcaccaaag tgaaggcgaa
      241 caacgtcttt gccgagtttg acggcgatat ggcggcgttt ttggaaaacg cgcaggcgca
      301 ggcggcggac atcgacaccg atttattgtg ggaagtgtgc ggcgaagagg aatttaccgc
      361 tgaagccata gccgaagaat attacggcca tgcgccgacc aaaaccgaac tggcggcgac
      421 cttgattgcg ctgtacgccg cgccggtgta tttctacaaa aaagccaaag gcgtgttcaa
      481 agccgcgccc gaagaaactt taaaacaagc acttgccgcc atcgaacgca aaaaacagca
      541 agacgcgcaa atcgatgctt gggcggaagc cttgaaacgc ggcgagatgc cgtctgaaat
      601 cgcggcggat ttgaaaacca tcctgcacgc gcccgacaag cagtcgctga cctacaaagc
      661 ctttaccaaa gccgccgacg cgctgaaaac ctctgcctac gaattggcga aaaaaacggg
      721 cggcattacg tccattcccc aatacctgca agacgggttt gaaatcaaat acttccctaa
      781 aggaacaggc ttccccgacc ttgcccttcc cgaaatgccc gacctgccca aggccgacgt
      841 taccgccttt tccattgacg acgaatcgac caccgaagtg gacgacgctt taagcctgac
      901 cgacttggac aacggcacga agcgcgtcgg catccacatc gccgcgccgt cgcttgccgt
      961 taaaccgggc gacaaaatgg aaaaaaacat catggaacgc ttaagcacgg tttattttcc
     1021 cggcggcaaa atcactatgt tgcccgaaaa ctggattgcc gcgttcagcc ttgatgcagg
     1081 tgcataccgc cctgccgtca gcatttattt cgatgtggac ggcgaattca acgtcggcga
     1141 accgacctgc aaaatcgaag cggtcaacat cgccgaaaac ctgcgtatcc aagccatcga
     1201 gccgcatttc aacgccgaaa ccggcttgga cgaagccggc gaaatgatgt tcgcccacca
     1261 tcaagacctg atttggttct atcaattcgc caccgccctg caaaaagcgc gcggcaaata
     1321 cgaacccgac cgcgcgccgc aatacgatta cagcatcgaa ttggatgagg aaggcaaggt
     1381 atccgtcgtc cgccgcgaac gcggttcgcc catcgatacg ctggtcagcg agatgatgat
     1441 tcttgccaac agcacttggg cgcaaatgct ccatgacaac gacctgcccg gccttttccg
     1501 cgtccaaccg gcaggcaaag tgcgcatgag cactaaatcc gagccgcaca tcggcatggg
     1561 cgtgcagcat tacggctggt tcacttcgcc gctgcgccgc gccgccgact acatcaacca
     1621 aaagcagctg atcagcctga tagacgacac tgccgagccg ctgtatcaaa acagcgatgc
     1681 cgagcttttc gccgcgctgc gcgactttga tgccgcctat accgcctacg ccgacttcca
     1741 acgccaaatg gaagcctact ggagcctggt ttacctgcaa cagcaaggca tcagcgagct
     1801 gacggcgacg attctgaaag aagacctcgt ccgcatcgaa ggcctgccgc tggtcacgcg
     1861 cgcgaccggt attccgtttg acgcactgcc caaatcgcag gcattgttca aaatcaccga
     1921 attagatgcc gagaagcagt ttgtctcgct caactacatc aaggcagtcg cacccgcggg
     1981 tacaacggca ggcaatgccg tctgaagccc gatacggcaa actccggcaa aaacagaaac
     2041 ctgaatctca tcattcccac agtcgggaat ccggtttttt gggtttccgt tgtttttccg
     2101 tttcaatgaa cttccaagcc gccgttcccg ataaataccc gcaatctaaa atcccgtcat
     2161 tcccacgcag atgggaatct aggtctgtcc gcacgggaac ttatgcgccg tcattcccgc
     2221 gaaagcggaa atccagaccg ttcggcttca gttttttggt ttcgggcaac ttctgaatcg
     2281 tcattcccgc gcaggtggaa atataggtct gtccgcacgg gaacttatac gccgtcattc
     2341 ccgcgaaagc gg
//
LOCUS       DAWHRA010000191         2344 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWHRA010000191 DAWHRA010000000
VERSION     DAWHRA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.191"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..906)
                     /gene="pheA"
                     /locus_tag="WIB30_001998"
     CDS             complement(<1..906)
                     /gene="pheA"
                     /locus_tag="WIB30_001998"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ3797999.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEIVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNR"
     gene            complement(945..2177)
                     /locus_tag="WIB30_001999"
     CDS             complement(945..2177)
                     /locus_tag="WIB30_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ3798000.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFVSSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
BASE COUNT          562 a          681 c          662 g          439 t
ORIGIN      
        1 ccggttgggc gcggaaacgg ccagcgaagt cttgtcgctg ccgcttgcac cggtttcgtg
       61 atgccccatc accaagaagc gcgtggtgtt gttcggttcg tcttcgatgc actcggcaac
      121 catatcgagt ccgtagattt ccgccgccgt gcgtccggcg atggcggcaa ccgtaccgtc
      181 gtccgattcg gcaaccagcc ttgcggcttc ggcattgctg gacacggcaa tccgttcggc
      241 gttgggcagg tgtctgccca accagtcgtt gcactgcgcc aacgcctgcg cgtgggaaaa
      301 gactttggca atgccttcgg tgctgccgtt gtttttacgc aaaaggttgt ggtggatgcg
      361 caaaacgatt tcgccgcacg cctgcaacgc ggtaacggca agcaggtcta acgtgcgacc
      421 gaccgagcct tcggtcgaat tttccacggg ggcgaccaga taatccgcct gacgcgtttc
      481 aacctgcttg aagcagtcgt ctatggtcgg acacgccatg gtgtgcgcgg cgtgtccgaa
      541 atgtttgatt gccgcctgct gggtaaacgt gccctgcggc cccagatagg cgatggtcag
      601 cgggcgttcg acggcgaggc actcgctcat cacttcccga aacaggcgtg ctaccgattc
      661 gtcgggcagc gggcctttgt tcaaatcctg aatgcggcgc aacacggcga cttcgcgttc
      721 ggggcggtac actgcgcccg tgcctttcag ctcgccgatg gcgtgggcgt gttgcgcacg
      781 ttcgttgagc aggcgcagga tttcggcatc gatggtgtcg atggcgttgc ggtggggaag
      841 gaggagttcg tcgatagttt gggacataat ttcgcctgaa cagtaattta tcgtgcattc
      901 taacatcaaa tgccgtctga aacggtttca gacggcattt tccgttaaag gtattcgctt
      961 tgcccgtttt ctttccacgc acgatgcgag cagagccaca gaagcgcaat gccgcaagag
     1021 gtggacgcgg tcatcgttgc cgccatcacg gttgccgaac cgtcgtgcaa gaaggtcgcc
     1081 gccataccca cccccgcgcc gattaaagat tggaatacac ccaatacggc gtttgcgctg
     1141 ccgccctctt ctttgaaata ggacataaaa cacgcctgcg tgtttgcacc gaccaagccc
     1201 tgcgtaccga cggaaaacat cacgcacgcg accagcagcc aaaacggggg caacccgaaa
     1261 aacagcacgg cggcgagttg ggacaggttg gcggcaaact ggacgacaat cccccacagc
     1321 aggatgcttt gcggatgcac gccggttttg agccgccacg cggtaacgcg gttgaaaaac
     1381 atcatcgtga tgatgttgag tgcaaacgcc caagcgtatt gatgaggcgt aacacggtag
     1441 agctgctggt acacgaagga agattcggtc agaaaggcga acatcgaacc gaagctgaat
     1501 gcctgaaaaa acagataacc catcgcagca cgggttttca atacgcgctt gaaccgcccc
     1561 gccaccagcc cgaacacgtc ccgtccgatt ttgccgccga cggcgggctt gggcaggaaa
     1621 tactgtacca aaccgagcag caccagcgaa tacgccgcca gaaaaacaaa aatcgcctgc
     1681 cagccaccca agccctgcaa caatgcgccg accatgggtg cgaccagcgg cacaaccatc
     1741 aaaatgatgc cgataagggc aaacatctgg gcggctttgc gtccggaata ataatcgcgc
     1801 accattgcgc cgacgatgac cacagtcatg cccgcaccga atgcctgcac gacgcgcagg
     1861 ttgaggagct gttcggcact cgaaacaaat acgatggcgg caacggcaag gcaatataca
     1921 atcaaaccgg tcagggcgac gggtttgcgc cctttgatgt cggacaccga accgccgacc
     1981 acctgtccga acgccgtgcc gaacataaac aaactcaaac tctgttcgat gcggtgaaca
     2041 tccgcgttca gcgattgcgc catttcggga atcgcgggca ggtaggcatc gatggaaaac
     2101 ggcatcagcg taaccagcat cgccatcaaa accgccatca gtttttcgct catttcagga
     2161 taatgggcag aaggcataaa ttctcgtccg attcgcaaac aaaccgcccg gcggcggaaa
     2221 acacaatacg cggaaacgga aaattttaac acaccggcac aaacaggcgg caacaaaagc
     2281 cccggcaaaa ccggacgaac cggaacgata tagtggatta acaaaaacca gtacagcgtt
     2341 gcct
//
LOCUS       DAWHRA010000192         2339 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWHRA010000192 DAWHRA010000000
VERSION     DAWHRA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.192"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(102..1427)
                     /locus_tag="WIB30_002000"
     CDS             complement(102..1427)
                     /locus_tag="WIB30_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ3798001.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDLAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1638..>2339
                     /locus_tag="WIB30_002001"
     CDS             1638..>2339
                     /locus_tag="WIB30_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEZ3798002.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIIVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLG"
BASE COUNT          437 a          567 c          751 g          584 t
ORIGIN      
        1 cgtcaaataa gaaaggaatt ttgtgtctgc ggtatcgcaa aacttcgcct taatgcgccc
       61 gattgcctag ggatgggctt cagatggcat tgttttccgg tttacgggcg gtattcgggc
      121 ttcataccgt tgggcagaag ctgccagaca tatcccgtgg ttttctgttt gccggcaagt
      181 tcgccggctt cgtcgccgta tccccaaaaa taatccacgc gcaccgcgcc tttaattgcg
      241 ctgccggtat cctgcgccat aatcaggcgg ttgagggctt tgcgggtaac cggatgggcg
      301 gtggcgacaa ataagggcgc acccaaggta atgtagtgcc ggtcgactgc gccggcatat
      361 tcccccatca gcggcgtgcc cagtgcgccg acagggccgt cattgccgct tccggtaagc
      421 tcgcggaaaa agatatagct ggggttttga cccaaaactt cggcgaggcg ttgcggattt
      481 tgccgcatat aagacttaat gccctgcatg gaggtttgtc cgagtttgag gtagccctta
      541 tccgccatgt attttccgat ggaaacatac ggatgctcgt ttttgtcggc atagccgatg
      601 cggatgtatt tgccggacgg ggttttcaga cggcccgagc cttggatgtg cataaaaaaa
      661 agttcgacag ggtcttcggc gtaaccgagt atcggggctt tgccgtcaag cgcgccgccg
      721 ttgatttggt tgcgcgtgtg gtaggggagg aagcggcttc cttcaaacct gcctttgatt
      781 gccgttgtgc gcgcggtgat ggggaatcgg gagaggtcgg cggtatgtgt gccgccggta
      841 ttgtcgattg tgccgctgtt tttccccgtc tgcctgatgc ggacaagggc ttttccgctc
      901 cgcaaaccgg caggcagggg gacggagata aaatcgtcgg gaataccgta aatcgggaag
      961 cgggcttgtg ccgtccgcct gtcgtcgccc ttcagcaccg gttcgtaata gccggtaacc
     1021 gtaccggcaa ggcttccgtt gcctgcaacc tgccacggcg tgaaatagcg ttcaaaaaac
     1081 tgttttgcct gaaaggaatg gacgggggtt tgaaaggctt gggcgcacac atcctgccag
     1141 ccttggcggt ttttcaaatt ggcgcagccg aggcggaagg attgcaggct tttggcgaaa
     1201 tcctgcgccg cccagtgggg cagggacagg tgcggtacaa cggtatagac ggccccgccg
     1261 ccgccgaccg tcgttccggc gaggtcgggg atgccgaccg gccggtccgg gccgttgatg
     1321 acggatgtgt cgggttgcgg aaaggtttgg atgctcttgc tttggcaggc ggcgaggatg
     1381 gcggcggcga tgccgtacag ggcggcgcgg aataggtatt ttttcataat ggacaatgtt
     1441 gccggcagta ataagaaaga tggtttcggg cggcgttgcg gcagccgtgg agaggggatt
     1501 ttaacacagg gcgcagctgc agcctgcgga actttccgcc gcgcggtact gcagataaaa
     1561 ataacttgca tttgtattta caagcaatga aaatattccg ataatatatt attcatcatc
     1621 cttgttcgtt cgcgtttatg ctggtcgctt ttttaattat gttgcgcgag ggtatcgaag
     1681 ccgcgctcat cgtcggcatc gttgccggtt ttctgaaaca gtccggacat tccaaactga
     1741 tgcctaaggt ctggttcggg gttgtccttg cttctttgat gtgtttgggg ctggggtacg
     1801 gcatccattc ggcaacaggc gagattcccc aaaagcagca ggagttcgtc gtcggcatca
     1861 tcggtttggt tgccgttgcc atgctgactt atatgatttt atggatgaaa aaggcggcgc
     1921 gttcgatgaa gcggcagctt caggattctg tgcaggcggc tttgaaccgt ggcagcggcc
     1981 aaggatgggc cttggtcggt atggcgtttc ttgccgtggc gcgcgaaggt ctagagagcg
     2041 tttttttcct gcttgccgta ttcaaacaga gcccgacgtg gcagatgccg gccggtgcgg
     2101 tagcgggggt tttggctgcc gccgtgattg gcgcgttgat ttatcagggt gggatgcgcc
     2161 tgaatctggc gaagtttttc cgttggacag gggcgtttct gattatcgtt gccgctggtc
     2221 tgcttgccgg ttcgctgcgt gcgctgcacg aagcaggcat ttggaacgcg cttcaggaca
     2281 ttgtgttcga ctcatcaaaa tatttgcacg aagacagccc gttgggcgtg ctgctcggc
//
LOCUS       DAWHRA010000193         2298 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWHRA010000193 DAWHRA010000000
VERSION     DAWHRA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.193"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(36..992)
                     /locus_tag="WIB30_002002"
     CDS             complement(36..992)
                     /locus_tag="WIB30_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3798003.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGDLADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAVALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            1228..2193
                     /locus_tag="WIB30_002003"
     CDS             1228..2193
                     /locus_tag="WIB30_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3798004.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAGKE"
BASE COUNT          490 a          612 c          732 g          464 t
ORIGIN      
        1 tgtttcaaat caaaatatct atgccgccgg ccccatcagc acttcttcac atccgaaggc
       61 aaaaatccgt aatgccgtct gaacgcttcg ttgaaccgtc ccgcgtggcg gtagccgcaa
      121 aagtgcatgg cggcttggac ggtgctgccg gattcgatga gggcgagcgc gtgttccagc
      181 cgcaggcggc gcaggcatcc ggcgacggtt tcgccggttt gcgctttgaa atagcgtttc
      241 aggtagcatt cgttcagtcc gacgcggcgg gcgatttcgg cgatggtcag cggacgggcg
      301 aattcgtgtt gcaggatgtc ggcggcttcg tctatgcgcc gacggcggta accgttgtcg
      361 tggcggcgga aggtgaagcg caataatcgg gcggagagtt ccagcgcggc ggcttcgtcg
      421 gcaagcaggc cgaagccgtc cgattcgaac gggcgttgca gcagtgggca ggccgccgcc
      481 gtcagtgcca ctgcgttttg cgccagccgt tgcagggcga atcggcctat tgtttgcggc
      541 gaaaacaggc gttcgtccag caagccttcg tcgtgccagc ggcgcagttt ttccagcgaa
      601 aaatccaaat gcagcgcgca catgccgctg ttgtcgggca gcagggtttc ggatacgtcc
      661 gccaaatcgc cgcgtaccag ccagatttcg ccggcagatg ggcggtattc cctgccgccc
      721 atttgtaacc ggttctgccc cgacaccatg acgaacaagg cgcagttgtg gctgaaattg
      781 tggatttcgg tgggaaacgc gcccgttccg ccgccgcgca tccgcgacaa ggtgatgccc
      841 gagtcgaagc ggttgatgca catttccaga tgcaaaccgg gctgttttgc ctgcgcaatg
      901 agcgcgctgt cggaacagcc gtccaacgcc cagccggatt tatcggagcg gacataggtt
      961 tggtactggc ggtagatggc ggcggtgttc atgattggat aggaacgagt tgtctaacaa
     1021 atgaattaaa taggaattat taccaataat caagcgcagg gattggttga aacggaaaag
     1081 gtcgtctgaa agggtgtttc agacgacctt ttccgtatcg ggaatttgtt ttgccgtatc
     1141 gggaattttg cgttttgcgg cgtggtttct gcaggttgtt tgcttaataa taaacattct
     1201 tattcgtatg caaaggaacc gcacaccgtg aaaccgcgtt tttattgggc agcctgcgcc
     1261 gtcctgctga ccgcctgttc gcccgaacct gccgccgaaa aaactgtatc cgccgcatcc
     1321 gcatctgccg ccacactgac cgtgccgacc gcgcggggcg atgccgttgt gccgaagaat
     1381 cccgaacgcg tcgccgtgta cgactgggcg gcgttggata cgctgaccga attgggcgtg
     1441 aatgtgggcg caaccaccgc gccggtgcgc gtggattatt tgcagcctgc atttgacaag
     1501 gcggcaacgg tggggacgct gttcgagccc gattacgaag ccctgcaccg ctacaatcct
     1561 cagcttgtca ttaccggcgg gccgggcgcg gaagcatatg aacagttggc gaaaaacgcg
     1621 accaccatag atctgacggt ggacaacggc aatatccgca ccagcggcga aaagcagatg
     1681 gagaccttgg cgcggatttt cggcaaggaa gcgcgcgcgg cggaattgaa ggcgcagatt
     1741 gacgcgctgt tcgcccaaac gcgcgaagcc gccaaaggca aaggacgcgg gctggtgctg
     1801 tcggttacgg gcaacaaggt gtccgccttc ggcacgcagt cgcggttggc aagttggata
     1861 cacggcgaca tcggcctacc gcctgtagac gaatctttac gcaacgaggg gcacgggcag
     1921 cctgtttcct tcgaatacat caaagagaaa aaccccgatt ggattttcat catcgaccgt
     1981 accgccgcca tcgggcagga agggccggcg gctgtcgaag tattggataa cgcgctggta
     2041 cgcggcacga acgcttggaa gcgcaagcaa atcatcgtca tgcctgccgc gaactacatt
     2101 gtcgcgggcg gcgcgcggca gttgattcag gcggcggagc agttgaaggc ggcgtttgaa
     2161 aaggcagaac ccgttgcggc ggggaaagag tagggagttg tctgaaaacg gagcttccga
     2221 aggaagcggg ggggtttctg cgaagctaaa gtacggtttc aacgaattga aaagcagcct
     2281 gtatgttgaa aataccgc
//
LOCUS       DAWHRA010000194         2249 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWHRA010000194 DAWHRA010000000
VERSION     DAWHRA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.194"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1480
                     /locus_tag="WIB30_002004"
     CDS             <1..1480
                     /locus_tag="WIB30_002004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449090.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ3798005.1"
                     /translation="SMRDALSLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGI
                     INQDGAALTAKAQEMAACAVGFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHR
                     LAQTISGEQIQLYYQIAVHGKRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVI
                     ENTELKSPSAQTAEKETAAKKPQPRPEAETAQTPVQTASAAAMPSEGKTAEPVTNQEN
                     NDIPPWEDAPDETAAGTAQASAKSIQTASEAGTPPKNQVSKNEAADNETDAPLSEVPS
                     ENPIQATPNNEALETEAFAHEAPAKPFNGYSFPNDDYLVEDGAEIPPPDWEHAAPADA
                     EEENNADESSNNEDHTPYAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPD
                     TGLMVLAMTAEARATADKKRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKR
                     VQAEDRQKAQALLEADPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    555..594
                     /locus_tag="WIB30_002004"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            1560..1895
                     /locus_tag="WIB30_002005"
     CDS             1560..1895
                     /locus_tag="WIB30_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ3798006.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
BASE COUNT          656 a          719 c          540 g          334 t
ORIGIN      
        1 atcgatgcgc gatgccttga gcctgctcga ccaagccatc gccctaggtt cgggcaaagt
       61 tgccgaaaac gatgtccgcc aaatgatcgg cgcggttgac aaacaatacc tttacgaact
      121 gctgacaggc atcatcaacc aagacggcgc agccctgacc gccaaagcgc aggaaatggc
      181 ggcgtgtgcc gtcggctttg acaacgcctt gggcgaactt gccatactgc tgcaacacct
      241 cgccctgata caggcagtgc cgaatgcctt ggcgcacgac gaccccgatt ccgatatttt
      301 gcaccgcctc gcccaaacca taagcggcga acaaatccag ctttactacc aaatcgccgt
      361 ccacggcaaa cgcgacctca gcctcgcccc cgacgaatac gccggcttta tgatgaccct
      421 gctgcgtatg ctggcgtttg cgcccttggc ggcagcatcg tgtgatgcaa atgccgtgat
      481 tgaaaatacc gaactaaaat ccccatcggc acaaaccgcc gaaaaggaaa ccgccgcaaa
      541 aaagccccaa ccgcgccctg aagcggaaac cgcccaaaca cccgttcaga cggcatccgc
      601 agcagcaatg ccgtctgaag gcaaaactgc cgaacccgtt accaatcaag aaaacaacga
      661 tattccgcct tgggaagacg cgccggacga aaccgcagcc ggcacggcgc aagcatcggc
      721 aaaaagcatt cagacggcat ccgaagccgg aacgccgccc aaaaaccaag tttccaagaa
      781 cgaagcagcc gacaacgaaa ccgatgcccc cttgtccgaa gtgccgtctg aaaaccccat
      841 tcaggcaaca ccgaataatg aagcccttga aacagaagca tttgcacacg aagctcctgc
      901 aaaacctttc aacggttaca gctttccgaa tgatgactac ctcgtagaag acggcgcaga
      961 aatcccaccg cccgattggg aacacgccgc ccctgccgat gcggaagaag aaaacaacgc
     1021 cgacgaaagc agcaacaacg aagaccacac gccatacgcc ccgccgcccg aattttccac
     1081 cgaaaactgg gcagccatcg tccggcactt cgcccgcaaa ctcggcgcgg cgcaaatgcc
     1141 ggcgcaacac tccgcgtgga cggaatacca tcccgacacc ggtctgatgg ttttggcaat
     1201 gaccgccgaa gcacgcgcca ccgccgacaa aaaacgcctc gacaaaatcc gcgacaccct
     1261 tgcccaagcc tacgggctgc aactcaccct gcaaacccaa gactggcgtg acgaagccgg
     1321 ccgggaaacc cccgcgatgc aggacaagcg cgtccaagcc gaagacaggc aaaaagcaca
     1381 agcattgctc gaagccgacc ccgccgcaca aaaaatcctc caagcattcg gcgcgcaatg
     1441 gcagcccgaa tcactggaat tggcggcaaa ccggccataa acagatataa tgccgcccga
     1501 acccttcgga cggcattgcc gtttccctta ttcaatcaaa acagacagga gtattcagta
     1561 tgttcggaaa agccggatta ggcggcctga tgaaacaggc gcagcaaatg caggaaaata
     1621 tgaaaaaagc gcaagccaaa ctcgccgaaa ccgaaatcga aggcgaagca ggcaacggcc
     1681 tggtcaaaat cacaatgacc tgcgcgcacg aagtacgcaa aatcgacatc agccccgatt
     1741 tgattcaaga agccgccgac gacaaagaaa tgcttgaaga cctcatcctc gccgccctca
     1801 aatccgcccg aggcaaagcc gaagaaaccg caaacaaaac aatgggcgca ttcacgcaag
     1861 gtctaccccc cggagtgggc gacttcttcc gctgatcccc gaccgtcatt cccacgcagg
     1921 cgggaatcta gaacgtagaa tctaagaaac cgttttactc gataaatttc cgtgccgagg
     1981 ggtctggatt cccgccttcg cgggaatgac ggcatcagtt tgcaagattc ggcgtgaacg
     2041 gtaaaaacag tgagaatgat aagaacgcaa aaacggcaag aatagcggga atcggcaggc
     2101 tgaagcccac cctaccatta tttacacatc cgtaccgctt aaatgccgtc tgaaacttcg
     2161 tcattcccgt gaaagcggga atccaacccc gtcggagcag aaacttacac cccgtcattc
     2221 ccgcgaacgc gggaatccag taaccgaaa
//
LOCUS       DAWHRA010000195         2213 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWHRA010000195 DAWHRA010000000
VERSION     DAWHRA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.195"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..202
                     /locus_tag="WIB30_002006"
     CDS             <1..202
                     /locus_tag="WIB30_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ3798007.1"
                     /translation="LQPLTESGISMTKFESRPSKSVLWEYLFFIDIEGHRRDAQIQTA
                     LERLGERASFVKVIGSYPTAVL"
     gene            239..1030
                     /gene="recO"
                     /locus_tag="WIB30_002007"
     CDS             239..1030
                     /gene="recO"
                     /locus_tag="WIB30_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ3798008.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLWVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSVSWYGSQELKTLHRAEWVGGWRQPQGRALFGGLYVNELVLK
                     LTAREDPVPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            1054..1782
                     /gene="pdxJ"
                     /locus_tag="WIB30_002008"
     CDS             1054..1782
                     /gene="pdxJ"
                     /locus_tag="WIB30_002008"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ3798009.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            1828..1935
                     /locus_tag="WIB30_002009"
                     /pseudo
     CDS             1828..1935
                     /locus_tag="WIB30_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          546 a          594 c          618 g          455 t
ORIGIN      
        1 gctgcaaccg ctgaccgaat cgggtatttc catgaccaag tttgagagcc gtccgagcaa
       61 atccgttttg tgggaatacc tgttcttcat cgacatcgaa ggacaccgcc gggacgcgca
      121 gattcagacg gcattggaac gcttgggcga acgcgcttcg ttcgtcaaag tcatcggttc
      181 gtacccgacc gccgttttgt agcggcggca gcgttcagac ggcatttccc caacgattat
      241 gtccgaatac cgagtcaacc atgaacccgt ttttatgctg gcatcttcgc cctggcgcga
      301 aagcagcctg tgggttgaag cattcagccg ccgttacggg cgtgtggctt tgctggcgcg
      361 cagcgcgcgc aaaaggcaga gcgagctgcg cggcgtattg gtgccgttcg tgcccgtcag
      421 cgtgtcgtgg tacggcagtc aggaactcaa aaccctacac cgcgccgaat gggtcggcgg
      481 ttggcggcag cctcagggca gggcgttgtt cggcggattg tatgtgaacg agttggtgtt
      541 gaaactgacc gcccgcgaag acccggtgcc cgagttatac gacgcgttgg cggaagtgat
      601 ggaggcggtg tgctgcaaag ccgcttatat cgacgacttg cgccgtttcg agtggcggct
      661 gctgaacctg ttgggcgttg cccccgattt gaaccgcgac ggggacggcg ggacgattgc
      721 ggcaggcggc acataccttg tccgcccgga aacagccgtc ttccccgtcg gaaaaggatt
      781 tgccgtaccg ccgcacgccg ccggcgttgt cgcccccggg cagagcctga tcgatttgcg
      841 cgaaggcagt ttccgcactg ccgaaagcct gcaacaggca ttgaaaatca cacggctttt
      901 tatccgccac ctgttgcccg aggggctgaa atcgcggcag gtgttggaac agatacggca
      961 gtttgaccgc aaagaaaccg cccgggaaac cgtcccgact tcggacggca cggcttcaaa
     1021 tgccgtctga aggcagaaat aaaaggaaag attatgcttt taggtgtcaa catcgaccac
     1081 atcgccaccg tccgcaatgc gcgcggtacg acttatccca gccccgtgga ggcggcactg
     1141 gttgccgaaa cgcacggtgc ggatttgatt accatgcacc tgcgcgaaga ccgccgccac
     1201 atcaaagacg cggacgtgtt tgccgtcaaa aacgccatcc gcacgcgcct gaaccttgaa
     1261 atggcgttga cggaagaaat gctcgaaaac gcgcttaaag tgatgccgga agacgtgtgc
     1321 atcgtgcctg aaaaacgtca ggaaatcacg accgaaggcg gcttggacgt attggcgcaa
     1381 caggaaaaaa tcgccgggtt caccaaaatc ctgaccgacg caggcatccg agtctctctc
     1441 tttatcgatg ccgacgacag gcaaatccaa gccgcctatg atgtcggcgc gcccgttgtc
     1501 gagctgcaca caggcgcgta tgccgacgcg cgcagccacg ccgaacaact caagcaattc
     1561 gagcgtctgc aaaacggcgc gcattttgcc ggcgatttgg gcttggtcgt caacgccgga
     1621 cacggactga ccatacacaa cgttaccccc atcgcccaaa tcctcgccat ccgcgaactg
     1681 aacatcgggc attcgctgat tgcccaagcc ctcttcctcg gactgcccga agccgtgcgc
     1741 caaatgaagg aggcgatgtt cagggcaagg ctgctgccgt aagggcaggc aaaccctttc
     1801 agacagcatt tcacgacagg gatatgttat agtggattaa atttaaatca ggacaaggcg
     1861 gcgaagccgc agacagtaca aatagtacgg caaggcaagc caacgccgta ctggtttaaa
     1921 tttaattcac tatatgaatc aaaagtatat tttatctgca aacaataata gtttgataga
     1981 agaaattcac aatacagtac agagtattgg gtattgtatt gttcgcggtc ttaatctaaa
     2041 ccatcttgat gacagccgga gaaacaagaa attatttgac tttctatctc aattaggaat
     2101 gctgacaaac cacaaagacg atggttttaa atctatattt tgggatatta aatattgagg
     2161 cgatgattat gtaatatagt ggattaacaa aaatcaggac aaggcgacga agc
//
LOCUS       DAWHRA010000196         2164 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWHRA010000196 DAWHRA010000000
VERSION     DAWHRA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.196"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            39..523
                     /locus_tag="WIB30_002010"
                     /pseudo
     CDS             39..523
                     /locus_tag="WIB30_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            619..1242
                     /gene="rsmG"
                     /locus_tag="WIB30_002011"
     CDS             619..1242
                     /gene="rsmG"
                     /locus_tag="WIB30_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ3798010.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLVYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1341..2114
                     /locus_tag="WIB30_002012"
     CDS             1341..2114
                     /locus_tag="WIB30_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ3798011.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVEEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELAARVSGK"
BASE COUNT          415 a          497 c          754 g          498 t
ORIGIN      
        1 ctatataatg cggtctgctt cggaagaggg ggacggcgat gtttgtgaac gagaaatatc
       61 cttatgcggc tctgtttgcg ggactggtgt ttttgacgct gccgtttgcg ttggcggtgc
      121 atgatgcctt tgcgcttgcg ttcggacgga cggggttgct ggtgtcggtg tcggacggcg
      181 gattcggctg gcgtggcggt tgggacggca ctgtttggtt tgtgttcggt gtgtttgcgt
      241 ttttgaatgt ggttgtgtcg gcgggtctga cgaaactggc gtacaaaaag atgatgcggc
      301 ggcattcgcg ttacacactg tttctgtcgg gcgtggcggc ttgcgcggcg gcagcggtgg
      361 cttggatttt cgagctgctg cttggcagtg gggctttggg cgggctgcgg gggaggcggt
      421 gttggaatat gcgtttgccg tgtggctggt ggcgatgctg acgctgccca aacgcctgac
      481 gcgcgcgccg gtgcagccgg tggtgtttca caggaaaaaa taggttggaa cgggaaatgc
      541 cgtctgaaac ccgacacgcg gtttcagacg gcatgttttt ccgctaacat tacgcctgaa
      601 tatggacagg aagcagatat ggaacgcaaa gaacgcctgc gtgcaggcat tgccgcgatg
      661 gggctggata tttcggaaac ggcgcaggac aggcttttgg tctatgtgga tttgttgaaa
      721 aagtggaaca aaacctacaa tctgaccgcc ctgcgcgacg aggaaaaaat gattgtccat
      781 catcttttgg acagccttac gctgctgccc catatcgagg gtgtgcaaac gatgctggat
      841 gtcggttcgg gcggcggtca gcccggcatt ccggcggcgg tgtgccgtcc ggatgtgcaa
      901 ataacccttt tggatgcgaa tacgaagaaa acggcttttt tacagcaggc ggttatcgag
      961 ttggggttgg acaatgtgcg cgtggtatcc ggacgcgtgg aggcggtttc ggacgtgcgt
     1021 gccgatgtgg ttaccagccg tgcgtttgca gaactggcgg attttgtgtc gtggacggtg
     1081 catctgttga aagacggcgg ctactgggcg gcgatgaagg gcgtgtatcc gcaggaagaa
     1141 atcggccgcc tgccgcagga tgtgtgcgtt gaaaaagtcc aaaggctcga cgtgccgggc
     1201 ttggatgcgg aacgccatat cgtcatcctg agcaagcgtt gagcgcactt cagacggcat
     1261 gaataccttt tttgtgcgga taaaggtaaa attccgcact gtttttcttt tttcaacatc
     1321 agacgggaca cgggcgggac atgagtgcga acatccttgc catcgccaat cagaagggcg
     1381 gtgtgggcaa aacgacgacg acggtaaatt tggcggcttc gctggcatcg cgcggcaaac
     1441 gcgtgctggt ggtcgatttg gatccgcagg gcaatgcgac gacgggcagc ggcatcgaca
     1501 aggcgggttt gcagtccggc gtttatcagg tcttattggg cgatgcggac gtgcagtcgg
     1561 cggcggtacg cagcaaagag ggcggatacg ctgtgttggg tgcgaaccgc gcgctggccg
     1621 gcgcggaaat cgaactggtg caggaaatcg cccgggaagt gcgtttgaaa aacgcgctca
     1681 aggcagtgga agaagattac gactttatcc tgatcgactg cccgccttcg ctgacgctgt
     1741 tgacgcttaa cgggctggtg gcggcgggcg gcgtgattgt gccgatgttg tgcgaatatt
     1801 acgcgctgga agggatttcc gatttgattg cgaccgtgcg caaaatccgt caggcggtca
     1861 atcccgattt ggacatcacg ggcatcgtgc gcacgatgta cgacagccgc agcaggctgg
     1921 ttgccgaagt cagcgaacag ttgcgcagcc atttcgggga tttgcttttt gaaaccgtca
     1981 tcccgcgcaa tatccgcctt gcggaagcgc cgagccacgg tatgccggtg atggcttacg
     2041 acgcgcaggc aaagggtacc aaggcgtatc ttgccttggc ggacgagctg gcggcgaggg
     2101 tgtcggggaa ataggtcaat ccaaatcggg ctgcccgtgc ctttatgctg tttggccggg
     2161 tgcg
//
LOCUS       DAWHRA010000197         2142 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWHRA010000197 DAWHRA010000000
VERSION     DAWHRA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.197"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(422..895)
                     /locus_tag="WIB30_002013"
     CDS             complement(422..895)
                     /locus_tag="WIB30_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798012.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEKYVFHTQADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
     gene            complement(984..1208)
                     /locus_tag="WIB30_002014"
     CDS             complement(984..1208)
                     /locus_tag="WIB30_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798013.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            complement(1478..1765)
                     /locus_tag="WIB30_002015"
     CDS             complement(1478..1765)
                     /locus_tag="WIB30_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798014.1"
                     /translation="MDDLIAAFLGYLSGKYREGKISRLGISLTAGSIFFVVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
BASE COUNT          700 a          445 c          324 g          673 t
ORIGIN      
        1 tgctacacct cgcataccat taatcagaat tcttcctatt ttttcgaatc cactatttct
       61 tccaaagatg cagcaaacgc aggttgagcc tagacccaat ctacgtttgc cgccgcatct
      121 gatccctatt gtttcttatc cttacatctt cctgccttgt caatcaaata aagacagaag
      181 actatacaaa aactgaccga catactgaaa atacctatcc ccttccatgc aatctccgca
      241 ccattgaccc aatagatcag actgagaaac aacaaagcca cagtataaat caacgtaaaa
      301 aatattgcgt aaagcacaat aaagggaact tttatctcac ggttgttttt tataatatat
      361 tcagcaactt gatttccgaa tatacctgat aaaaaatata gtattagatc atccatttcg
      421 tttatcttct atgttttccc attgcggcgc tagaagactg atttaatgct gcaccatttg
      481 cacgaataac tgcattagga atagcatttc cccctttcca agcagaacgg gcaaaaacac
      541 tagtagtaat ccctgcaccg cgcaacatcg cactgctata tccgcctcct atcataccac
      601 cagcggttgc agccaaggta cttcttgttg aagcaaattg ccctgtttta attttagaaa
      661 tgccgtgatt agcccaagca ctaattgccc ctcccatcaa agcacctgcc acaataggaa
      721 gaaacgcccc ctcagtctcc ttcatctcct tttgagaaag ctccgccaac tgcatcggcg
      781 catctgcctg cgtgtggaat acgtattttt caaatgcctg attgtccaat ccgtttgcca
      841 ttgcgggggc gattatagac agcatcatcg tggcttcagt gatttgtttt ttcataacaa
      901 attcttaggg aggagataat gttccatcaa aatcctcctg aaaggctgcc tgaaatttcc
      961 aagcagcctt tttgattgct taattaatcg gatagtgcga caaatataaa gtgggcggca
     1021 atcattgccg taacggcagc cataactgtt ttattttctt caaaccaaag caagttgaga
     1081 aacataaaca tcaagaaata tgatggttct tcagttttgc cgctgctttc ttgagttaga
     1141 agataaaata tagatatggc ggttatgccg attgctgctt ttattaaaat atatttgttt
     1201 ttcatcatct tgtatggttt ttcatgcaag tttcaacaat aaaattcgga ataagataga
     1261 gagatgttga tatttcagat aaatatacaa aacaatcaag aaactattgg cggtatgtat
     1321 aatcttgaat ttcttttttt gatgaaatta aaataatgaa atatatggta aacgttacaa
     1381 cggaagcctt agcctcccga ttgcagtatt tcaattccaa gataaatgcc gcatagaaga
     1441 taaaatactc aaatggtttt ttagcggctt aaatatctta tttaatctta ttgattgttt
     1501 tcccaatggt ttccgcaata aataataaga agtagaataa taaacaaaca gaacatgaaa
     1561 ataaaaataa atctttcatt atcccagtaa taaaactcca aagattttcc ttatttccat
     1621 caacaataaa aattatcagt tcatgaataa agaatactac aaagaaaatg cttcctgccg
     1681 ttagagatat tcctaacctg cttatttttc cttctctata tttacctgat aaatagccta
     1741 aaaaagctgc aatcaaatca tccattttaa aaatttctgt aaaaacaggt aatgtcttta
     1801 attcattacc tgttttgcca ctatatctat gatataagcg tctcttaccc ttcagcctct
     1861 ttgggaaatc aaagccctgc ttagaacgta tgttgcacac ccaatttcaa taggtaaatc
     1921 agattgcaaa tccagcaatt tgaatattgt cattgttccg tgcaaaaggg atttttattg
     1981 atgagttgtg tactgggttt cagcttggct ttttagataa tcttttcaca agaacataca
     2041 tcaatactgt ccaaaaaaaa agatttgata aatatattgc aacatatttt attccacctc
     2101 catccgaaac agaaaccaat atatttttaa ttaatacata gc
//
LOCUS       DAWHRA010000198         2138 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWHRA010000198 DAWHRA010000000
VERSION     DAWHRA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.198"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            429..1886
                     /locus_tag="WIB30_002016"
     CDS             429..1886
                     /locus_tag="WIB30_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3798015.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          499 a          624 c          495 g          520 t
ORIGIN      
        1 aaggcggcga agccgcagac agtacaaata gtacggaacc gattcactcg gtgcttgagc
       61 accttagaga atcgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa
      121 tccactataa tgcgcccttc ggcgtggcgg atatataagg aagtgatttt ccatctaagt
      181 aaaaaccgcc ctatcggata agcccttaac agaaaaggct ttacccgcgc cgtatcggaa
      241 cacatcctct aaaatacaat ccgttgaatt gaaaaaaata taaaaacatc cgcccgcgaa
      301 aaacggcagc gcgtcgtttg acaaagaatg aaaatatcgg ttaaaaaccg attttcatac
      361 aaaaaacacc gccgccgtcc gcatccgttt cagacggtat tgagagaaaa tcttttagga
      421 gaacctttat gtcccggcat cccgccccca ccggagaaaa aacattcttc ggccacccct
      481 tccagctttc caccctcttc catatcgaat tgtgggaacg tttttcattt tacggaatgc
      541 agggcatcct gctgatttac ctctactaca ccgccgacaa aggcggcttg ggcatagaca
      601 aaaccctcgc cggcggcatt gtcggcgcat acagcggcag cgtgtacctg tccaccattt
      661 tgggggcgtg gtttgccgac cgagtatggg gtgcggaaaa aaccctcttc ctctcgggca
      721 tcgtcgtgat gctcggacac atcgtccttg ccgccgcccc gggcctgtac ggccttttaa
      781 tcgggctgat attcatcgca ttgggcagcg gcggcgtgaa atctacggcc agttctatgg
      841 tgggcgcatt atacgaacag gacgaaatgc gcccgctgcg cgatgcggga ttttccattt
      901 tctacatcgc catcaacatc ggcggcttcc taggcccgct gctgaccggc ctactgcagg
      961 aaaacatcgg tttccattat ggtttcggcg cggcggcggt cggtatggca ttcggcttgt
     1021 ggcgttattc cctgggacgt aaaaacctgc cccaccccac cgtcccccat ccgctttcaa
     1081 aaggacaggg caaaactgcg gccgccgtcg gcatcgccct catcgccgca cttgcaaccg
     1141 ccatcaaaac cgggcttgtc aacctcgaca atttctccgg catcctatta tctaccgtca
     1201 tccttgccgt catcgcctat ttcgcccgcc tgctgaccaa cccccgcgtc agttccgaca
     1261 acaaacggca catcatcgcc tacatcccgc ttttcctgac catctgtatg ttttgggccg
     1321 tctggtttca gatttacacc gtggcaaccg tctatttcga cgaaaccgtc aaccgcacca
     1381 tcggttcgtt taccgtgccc gtcgcttgga aagattctat gcaaagcctg tgggtcatcc
     1441 tgttttccgg actgatggcg gcaatgtgga caaaaatggg gcgcaaacag cccaaaaccc
     1501 cgctgaaatt cgctatggcg gtatttgtta ccggcgcgtc gtttttggga ttcgtcccct
     1561 ttatttcctc cggtacgccg atgcctattg cggttttcgc actgatcgtc ctcgccatca
     1621 cgataggcga actgatgatt tccccgattg cgctgtccat ctccaccaaa atcgcaccgc
     1681 ctttattcaa aacccaaatg gtcgccctta atttccttgc cttttcatta ggcttcactt
     1741 tgggcggcgt attgtttgaa aaaggctatc aggcgggcga cgaaatcggc ttctatcggc
     1801 tgctgttcta catcggcgca gccacaggct tcctgctgct cctgctcgtc cccaaattga
     1861 acaaaatgct cgaaggcaca gactaagtcc cgccccgatg ccgtctgaac ccttcagacg
     1921 gcatttttcc gcataatgaa accaaaccgt ttccacccga caggacaggc tcccgcccaa
     1981 ccggaaggca gcctgccgat tgtcatttga ataacgcaag ggaaagccgt tgatttccgt
     2041 ttgtatggaa acagtttggt ttcattggaa aaaggcattt tgtccgacta aattagtgct
     2101 gcatcaacga aatatatagt ggattaacaa aaatcagg
//
LOCUS       DAWHRA010000199         2124 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWHRA010000199 DAWHRA010000000
VERSION     DAWHRA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.199"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(339..761)
                     /locus_tag="WIB30_002017"
     CDS             complement(339..761)
                     /locus_tag="WIB30_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ3798016.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(879..1550)
                     /locus_tag="WIB30_002018"
     CDS             complement(879..1550)
                     /locus_tag="WIB30_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ3798017.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(1566..>2124)
                     /locus_tag="WIB30_002019"
     CDS             complement(1566..>2124)
                     /locus_tag="WIB30_002019"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ3798018.1"
                     /translation="HNVRIAAVLSNSETAAGLQWAAERGIPTDSLNHKNFTSRLAFDT
                     AMMEKIDAYQPDLVVLAGFMRILTPEFCARYEGRLMNIHPSILPSFTGLHTHERALEA
                     GCRVAGCTIHFVTAELDCGPIVSQGVVPILDGDTADDIAARVLAVEHKLYPKAVADFA
                     AGRLIIEGNRVRNSENADAARFLTA"
BASE COUNT          493 a          576 c          534 g          521 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataatt caataaatta atatatggct taaaataacg
       61 ggattctcgc ctcccgcccg cccgcagaag caggcgggta tcattttaaa acgcggcatt
      121 taaaatttga ccgaaaattg ttgacaatcc ggaatcaagt ctgcacaata ccccgacaag
      181 tccaagtatt ataaaggctg aataaagagg aaacagcagg cagatatatt cgggaggtgc
      241 agtccgaata tatctgcttt tttatgcgcc tccggattgc ctgccgcacc tttcccttca
      301 gacggtatca gccgtttccc cataatgccg cccgatgcct atttatctgc cccggcaatt
      361 tcaaaactgt gggtaatctt tgccgctttg cccaacataa tcgaagccga acagtatttt
      421 tcggcagaca tctgaacggc gcgctcaatg gccgattctt tcaaatcatg cccgaatact
      481 ttgaaatgga tgtggatttc ggtaaacacg cgcggcgcat cgtccgcccg tttcgccgta
      541 accgtcgcac ggcagtcagt cactttctga cgctgttttt cggcaatcat caccacatcg
      601 atgctcgaac agcccgccac gcccaacagc agcatttcca aagggctggg cccgcgctta
      661 gccttacctt ctgccgccga cccctccata acgacgctgt gcccgccttc cgtcgtgccg
      721 acaaaacaca tcccgtctat ccattttgat gtaacctgca tggtgtcatt cctgaaaata
      781 gcgttaaaac cgctctgcat atggcgttat tgtaaacaat ttcaagcggc ttatgcagaa
      841 atatggacaa aacggcaaaa aaacacttga aaaccgattt acggtttggc tgcctggccg
      901 ttgatctgca ccgatttgag tttcagcgta taggttttgc cgtcgtcggt atagccgatt
      961 tgtgccggaa tattgttcag ggacggtgcg aagaaataca ttaccgcatc gtcgccgcgc
     1021 cgcacccgat atttgacgac ttcggtttcc acgccgccta tgctgtattt tcctgtaccc
     1081 gccttattca aaccgccgac ggaataaagt tttttgccgt tggtgatttt cagccccggg
     1141 gggagtttcg cgtcatttgc cgccaactgc caggcaagcg tgaacaaatc catagccttg
     1201 gggctttgct cggttttgct ctcgcccgct ttgccgtaag ttacgctgcc gtcggcgaat
     1261 ttggcttccg catacagttt gcccctgcgt atgtctctat agtaggtagg gtgcagggta
     1321 ttgccgacaa ccgtaccgcc ggactcgaaa cggatattgt atagcggcac tttaatcgtc
     1381 gaaacgattt tgtaagcatt gccgctgcgt tcaaatgtca tcgtggcggg aatgccgtag
     1441 ctgccggaat agtgcagcac ggcggattgg ggcagccctg ccgcatacgc gcacggcagg
     1501 gcggcggaca aaatggcggc ggaaaatata tttttaaaag tcttcatcat ttgctcccgc
     1561 ccggtttacg ccgtcagaaa acgggcggca tcggcgtttt ccgaatttct gacgcggttt
     1621 ccctcaataa tcaggcggcc ggcggcaaaa tcggcaacgg ctttcggata aagtttatgc
     1681 tcgacagcca aaacccgtgc ggcaatatcg tctgccgtat cgccgtcgag tatcggcaca
     1741 accccttgcg atacaatcgg gccgcaatcc agttcggcag taacgaaatg gatggtgcag
     1801 ccggcaacgc ggcagcccgc ctccaaagcg cgttcgtgcg tatgaagtcc ggtaaacgag
     1861 ggaaggatgg acgggtgaat gttcatcagc ctgccttcgt aacgggcgca aaactcgggg
     1921 gtcagaatcc gcataaaacc tgccaaaacc accaagtcgg gttgatatgc gtcgattttc
     1981 tccatcatgg cggtatcgaa ggcaagccgg gatgtaaagt ttttatgatt caggctatcg
     2041 gtcgggatgc cgcgttcggc cgcccattgc aaaccggcag ccgtttcgct gttgctcaac
     2101 acggcggcaa tgcggacgtt gtga
//
LOCUS       DAWHRA010000200         2118 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWHRA010000200 DAWHRA010000000
VERSION     DAWHRA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.200"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1032
                     /gene="rfbB"
                     /locus_tag="WIB30_002020"
     CDS             <1..1032
                     /gene="rfbB"
                     /locus_tag="WIB30_002020"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ3798019.1"
                     /translation="IGSAVVRHIIRNTQDSVVNLDKLTYAGNLESLTDIADNPRYAFE
                     QVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARA
                     YWHDMPPEKKAAFRFHHISTDEVYGDLHGTDDLFTETAPYAPSSPYSASKASSDHLVR
                     AWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVE
                     DHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICTLLEELVPEKPAGVARYEDLI
                     TFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQWYLDNKTWWQNVLNGSY
                     RLERLDTGK"
     gene            1106..1972
                     /gene="rfbA"
                     /locus_tag="WIB30_002021"
     CDS             1106..1972
                     /gene="rfbA"
                     /locus_tag="WIB30_002021"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ3798020.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLRQAAAKKHGATVFAYQVKNPERFGVVEFDANFRALSVEEK
                     PQQPKSDWAVTGLYFYDNRAVEFAKQIKPSARGELEITDLNQMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKPLEKT
                     AYGQYLLRLIGK"
     gene            2017..>2118
                     /locus_tag="WIB30_002022"
     CDS             2017..>2118
                     /locus_tag="WIB30_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEZ3798021.1"
                     /translation="MNIIDTALPDVKILKPQVFTDARGFFMETFRDGW"
BASE COUNT          530 a          609 c          514 g          465 t
ORIGIN      
        1 atcggctctg ccgttgtccg tcatattatc cgaaacaccc aagattccgt cgtcaacctc
       61 gacaaactga cctacgcggg caatctcgaa tcgctgaccg acattgccga taatccccgc
      121 tatgctttcg agcaagtgga catttgcgac cgcgccgaac tcgaccgcgt attcgcacaa
      181 caccggcccg atgccgtgat gcacttggcg gcggaaagcc atgtcgaccg ctctatcggt
      241 tcggcaggcg agtttatcca aaccaatatc gtcggcacat tcaatctgct ggaagcggcg
      301 cgcgcttatt ggcacgatat gccgcctgaa aaaaaagcgg ctttccgttt tcatcatatt
      361 tcgactgatg aagtgtacgg cgatttgcac ggcacggatg atttgtttac cgaaaccgcg
      421 ccctacgcgc cgtccagccc ctactctgcc tctaaagcgt ccagcgacca cctcgtccgc
      481 gcgtggttgc gtacttacgg cttgccgacc attgtaacca actgctccaa caactacggt
      541 ccttaccatt ttccggaaaa actgattccg ctgatgattc tgaacgcgct tgacggcaaa
      601 ccgctgcctg tgtacggcga cggtatgcaa atccgcgact ggctgtttgt cgaagaccac
      661 gcgcgcgcac tgtatcaggt tgttaccgaa ggtgttgtcg gcgaaaccta caatatcggc
      721 ggccacaatg aaaaagccaa tattgaagtc gtcaaaacca tctgcaccct gctggaagag
      781 cttgttcctg aaaaaccggc cggtgtggcg cgttatgaag atttgattac tttcgtacaa
      841 gaccgccccg gccatgacgt acgctacgcc gtcgacgcag ccaaaatcag gcgggatttg
      901 ggctggctgc ctttggaaac cttcgagtcc ggcctccgca aaaccgtgca atggtatttg
      961 gacaacaaaa cctggtggca aaatgtattg aacggcagct atcgtttaga gcgtttagac
     1021 accggaaaat aagttttcag acggcctgct cccatattag gccgtctgaa aatcatgcaa
     1081 acacacacta ctcaggagaa aacccatgaa aggcatcatc cttgccggcg gcagcggcac
     1141 gcgcctctac cccatcacgc gcggcgtgtc caaacagctc ctgcccgtgt acgacaaacc
     1201 gatgatttat taccccttgt cggttttgat gctggcggga atccgcgata ttttggtgat
     1261 taccgcgcct gaagacaacg cctctttcaa acgcctgctt ggcgacggca gcgatttcgg
     1321 catttccatc agttatgccg tgcaacccag tccggacggc ttggcacagg catttatcat
     1381 cggcgaagaa tttatcggca acgacaatgt ttgcttggtt ttgggcgaca atatttttta
     1441 cggtcagtcg tttacgcaaa cattgcggca agcggcggca aaaaaacacg gcgcgaccgt
     1501 gtttgcttat caggtcaaaa atcccgaacg tttcggcgtg gttgaatttg acgcaaactt
     1561 ccgcgccctg tctgtcgaag agaaaccgca acagcccaaa tccgattggg cggtaaccgg
     1621 cttgtacttc tacgacaacc gcgccgtcga gttcgccaaa caaatcaaac cgtccgcacg
     1681 cggcgaattg gaaattaccg acctcaacca aatgtatttg gaagacggtt cattgtccgt
     1741 ccaaatatta ggacgcggtt tcgcatggtt ggacaccggc acccacgaga gcctgcacga
     1801 agccgcttca ttcgtccaaa ccgtgcaaaa tatccaaaac ctgcacatcg cctgcctcga
     1861 agaaatcgcc tggcgcaacg gttggctgac gaaaaaagat gtggaaacac gggcaaagcc
     1921 tttggaaaaa accgcctacg ggcaatacct gctgcgccta atcggcaaat aaatgccgtc
     1981 tgaaccactt tcttcagacg gcatacagag aaaatcatga acatcatcga caccgccctc
     2041 cccgacgtaa aaattttaaa accgcaagtc ttcacagacg cgcgcggctt ttttatggaa
     2101 accttccgcg acgggtgg
//
LOCUS       DAWHRA010000201         2116 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWHRA010000201 DAWHRA010000000
VERSION     DAWHRA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.201"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            123..1508
                     /locus_tag="WIB30_002023"
     CDS             123..1508
                     /locus_tag="WIB30_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ3798022.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1528..2046
                     /locus_tag="WIB30_002024"
     CDS             1528..2046
                     /locus_tag="WIB30_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ3798023.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTKQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          443 a          646 c          573 g          454 t
ORIGIN      
        1 ttcaaataaa caggaatata acttcagaca aacaacttac cgcccgattt gtgctatcgt
       61 tttcgcacaa cttaaaaaaa cctgacaatt ttgtactttt attacagaga aaggctttac
      121 aaatggacgg ctggacacag acgctgtccg cgcaaaccct gttgggcatt tcggcggcgg
      181 caatcatcct cattctgatt ttaatcgtca aattccgcat ccacgcgctg ctgacactgg
      241 tcatcgtcag cctgctgacg gctttggcaa ccggtttgcc cacaggcagc attgtcaacg
      301 acgtactggt caaaaacttc ggcggcacgc tcggcggcgt ggcgcttctg gtcggcctgg
      361 gcgcgatgct cggacgtttg gtcgaaacat ccggcggcgc acagtcgctg gcggacgcgc
      421 tgatccggat gttcggcgaa aaacgcgcac cgttcgcgct gggcgttgcc tcgctgattt
      481 tcggcttccc gattttcttc gatgccggac taatcgtcat gctgcccatc gtgttcgcca
      541 ccgcacggcg catgaaacag gacgtactgc ccttcgcgct tgcctccatc ggcgcatttt
      601 ccgtcatgca cgtcttcctg ccgccccatc cgggcccgat tgccgcttcc gaattttacg
      661 gcgcgaacat cggccaagtt ttgattttgg gtctgccgac cgccttcatc acatggtatt
      721 tcagcggcta tatgctcggc aaagtgttgg ggcgcaccat ccatgttccc gttcccgaac
      781 tgctcagcgg cggcacgcaa gacaacgacc tgccgaaaga acctgccaaa gcaggaacgg
      841 tcgtcgccat catgctgatt cccatgctgc tgattttcct gaataccggc gtatcggccc
      901 tcatcagcga aaaactcgta agtgcggacg aaacctgggt tcagacggca aaaataatcg
      961 gttcgacacc gatcgccctt ctgatttccg tattggtcgc actgtttgtc ttgggacgca
     1021 aacgcggcga aagcggcagc gcgttggaaa aaaccgtgga cggcgcactc gcccccgtct
     1081 gttccgtgat tctgattacc ggcgcgggcg gtatgttcgg cggcgttttg cgcgcttccg
     1141 gcatcggcaa ggcactcgcc gacagcatgg cggatttggg cattcccgtc cttttgggct
     1201 gtttccttgt cgccttggca ctgcgtatcg cgcaaggttc ggcaaccgtc gccctgacca
     1261 ccgccgccgc gctgatggct cctgccgttg ccgccgccgg ctttaccgac tggcagctcg
     1321 cctgtatcgt attggcaacg gcggcaggtt cggtcggttg cagccacttc aacgactccg
     1381 gcttctggct ggtcggccgc ctcttggaca tggacgtacc gaccacgctg aaaacctgga
     1441 cggtcaacca aaccctcatc gcactcatcg gctttgcctt gtccgcactg ctgttcgcca
     1501 tcgtctgaca gacggaaagg atagtaaatg actacgcatt ttgtcgttat gggcgtatgc
     1561 ggctgcggca agaccaccgc cgcgctgtcc ctgcagaaac acctcggtca atgtccctat
     1621 gccgaaggcg acgagttcca cacccaagcc aaccgcgaca agatgggcgc gggtattccg
     1681 ctgaccgatg aagaccgcta tccgtggttg ggcaatctgc gcgactggat gacgaaacag
     1741 gcgcaaaacg gcgcggacca caccatcgta acctgttccg ccctcaaacg cggctaccgc
     1801 gacattctgc gcggagccga aggcaaagct gccttcatcc acctcagtcc gccgcaagac
     1861 atcaacctcg agcgcatgat gtcgcgcaaa ggacattaca tgaaagcagg gatgctcgat
     1921 tcgcaactgg aaatcctcga ggaactgggc gaaggcgaat acggggtcaa aatcgccaac
     1981 cccggcacgc ccgaagcggt cgaagccgat attctgaact gggttgcctc ggaaaacctg
     2041 ctttgaagca atatgccgtc tgaagcccga cacaggatgg gtttcagacg gcataaacat
     2101 cgggaacaga atggat
//
LOCUS       DAWHRA010000202         2043 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWHRA010000202 DAWHRA010000000
VERSION     DAWHRA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.202"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(331..654)
                     /locus_tag="WIB30_002025"
     CDS             complement(331..654)
                     /locus_tag="WIB30_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ3798024.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(642..881)
                     /locus_tag="WIB30_002026"
     CDS             complement(642..881)
                     /locus_tag="WIB30_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ3798025.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(1004..1801)
                     /gene="truA"
                     /locus_tag="WIB30_002027"
     CDS             complement(1004..1801)
                     /gene="truA"
                     /locus_tag="WIB30_002027"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ3798026.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          463 a          485 c          579 g          516 t
ORIGIN      
        1 accgtcattc ccgccacttt tcgtcattcc cgctcaggcg ggaatctaga atctcggact
       61 ttcagataat ctttgaatat tgccgctgcc ttaaggtctg gattcccgcc tgcgcgggaa
      121 tgacggctgc agatgcccga cggtctttag agtggattaa caaaaatcag gacaaggcga
      181 cgaagccgca gacagtacaa atagtacgga accgattcac ttggtgcttc aacaccttag
      241 agaatcgttc tctttgagct aaggcgaggc aacgccgtac tggtttttgt taatccacta
      301 taatgaacac aatccattca gactattcaa tcaggcaaac atctcctgca atactgcaaa
      361 cagtttttca gccgtactgt tgtctaaatt tccaagatgt ttgaccaatc cggctttatc
      421 cacagcccta atctgttcgg gcaaaagcaa accgtcttta tcctgaaagc ggacattgac
      481 gcggaacggg gcaggacggc ttccgctcgt catgggaacg atcagcacag tcttgagata
      541 gttgtgtatt tcaggaggag agactacgac acaaggacgt gtctttttga tttcgcttcc
      601 tacggtcggg tctaaggaga ccagatagat tccgccgcgt actaccatat ccattcttta
      661 tccgcttcgt tttcaatttc ggaaaaaaaa tgctcctgct cggtttcgac aagcattgcg
      721 gcagcttctg cccatcccct gcgaacggta ggacagctta aaataatatt gcccttttca
      781 actgtaacag ccaagctgtc tactgcccct atttgaccca ataatgattt gggcagaatc
      841 acgccttgcg agtttcccat tttgcgtatg ttgagaatca tatacgtacc ctatttacca
      901 gtttatgtaa taacaatgtt attaccttga tgaggtagtg tcaatattga aaaagattgc
      961 ctgacagttt gtccgatttc aaaatctccg cgacaagaat gttttaaagc cattcgggga
     1021 tttgggggcg gatgatgccg tatgcctcgg gatagtcgac gccggtcagg tagagtccgt
     1081 cgggcatgaa ggtcggtggg gctttgaggc ggctgcgttc ttgaatcagt gcggcgaagc
     1141 cttcgacgct gagtctgccg ctgccgacat aaacgagcgc gcccatgatg ttgcgtacca
     1201 tatggtgcaa aaaggcgttg ccgtgcaaat cgaggcggac gagtcctgag ctttgggtaa
     1261 ggtcggcgcg gtagatggtt ttgacggggg attttgcttg gcattcggcg gcgcggaagc
     1321 tggagaagtc ttgttcgccg atcaaatagg cggcagcccg acgcatcggt tcgatgtcga
     1381 gtttgaggtg tgtccagcct gccctgtttt tgagcagggg ggaacggacg ggggcggatt
     1441 cgagcaggta gcggtagtgc cgtccgtatg cgtcgaagcg ggcgtgaaat tcgggggcga
     1501 cctgtcgggc gtgcaaaacg gcaatgcctt cgggcaggtg ggcatttacg ccgcgcaccc
     1561 atgcctgttg gggacgggcg gcagttgtgt cgaagtggac gacttgggcg gtggcatgca
     1621 cgccggtgtc ggtcctgccg gcaacggtgg tggaaaccgc ttcccctgct atttgggcga
     1681 gcgcggtttc caatgccgcc tgaacggtcg gtacgccgtc agcctgtttc tgccagccgt
     1741 aaaagcggct gccgtcatag gatagggtta ttgcccagcg ttgtttttgt gcggtatcca
     1801 tcggatttgg gattcggata aatgttcaga ctgcattgta tcgcagattt tgcagggaaa
     1861 cggcaaacgc ccagggcgag cggcgttgtt tggggagttg ggggggtgga tgcagttgct
     1921 acgaatcgct atcctgtgaa ttcaccctgt caggagtgcc cgaatcgtca ttcccgcgca
     1981 gtcgggaatc taggacgtaa aatctaaaga aaccatttta tagtggatta acaaaaacca
     2041 gta
//
LOCUS       DAWHRA010000203         1883 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWHRA010000203 DAWHRA010000000
VERSION     DAWHRA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.203"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(127..1437)
                     /locus_tag="WIB30_002028"
     CDS             complement(127..1437)
                     /locus_tag="WIB30_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ3798027.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYSA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
BASE COUNT          450 a          481 c          494 g          458 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagcc
      241 gtcgggcata tagctttcgc cggcggagta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcatcag cttggtcggg gcgttgcgca ggtggacggg
      361 cacttcgtcg ctagcgtttt ctttgacgaa gtggcgcatt tggttgtatg ccttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaagata caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt ggaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcggtta agtttttcag
      781 acgacgtgta tcggcggcgc gtaaaagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaagggttg
      961 caaaacatac acctgagcgc ggctcaacag cgcgggattg acttcaaacg acggattttc
     1021 cgtcgtcgca ccaataaagg ttagcaaacc gctttcgaca tgcggcaaaa acgcgtcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc gtccctgctg
     1141 caaagcgatt tcggctttat cgattgcctc gcggatgtcc ttcacgccgg aaaatacggc
     1201 ggaaacaggc aaaaactggg cgttgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccgcctt ctaccgccac
     1321 gcgcaaaggt ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcatcggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gatttaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aagagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 gtgaaaactt tagaaaatta gaa
//
LOCUS       DAWHRA010000204         1818 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWHRA010000204 DAWHRA010000000
VERSION     DAWHRA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.204"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1158
                     /locus_tag="WIB30_002029"
     CDS             <1..1158
                     /locus_tag="WIB30_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ3798028.1"
                     /translation="LAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAAL
                     LKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLA
                     AQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGTA
                     LRLDRLETAVSGLPLMLDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRLE
                     NNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKSP
                     ATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPAN
                     EKTASEPAAVSDVKTIEAPSLPSERKPEQPAKKQTVPEKAGRSPSAKGERA"
     gene            1155..>1818
                     /locus_tag="WIB30_002030"
     CDS             1155..>1818
                     /locus_tag="WIB30_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ3798029.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGA"
BASE COUNT          456 a          476 c          523 g          363 t
ORIGIN      
        1 ttggcggcat tgggtttggg tacaagtggt tttttgtttg tccaaggaca gaatgtcttg
       61 aaaaaccaag agctggcatt caaccaaaaa atcgacaaag ccgccttggg cgagtcggaa
      121 aacgccgccc tgttgaaaga caacctcaac cggcaagccg ccatacaatc agagctcgac
      181 cgtttggacg gaaacgtcaa agcaaacggc gaacaaatct tggaaatgca aaaatcctat
      241 cgcgagttga ccaaaggacg cgccgattgg ctggtggacg aaaccgagac catactcaat
      301 ctggcggcgc aacagctggt gttgactggc aatatccaaa cggcagtcgg cgtattggag
      361 catatcgaca gccgcctgtc ccgtttcaat caggcagagc ttctgccgat caagcaggcg
      421 gtcagcagcg acttggcgga actgaaaaac cgtccctatg tcgatatttc cggcacggca
      481 ttgcgcctcg acaggctgga aaccgccgta tccggactgc cgctgatgct cgacggcgtg
      541 ctgaaaccgg gcgtacaggt gaagaacgaa gccgcttccg cttcatggtg gcagaacgta
      601 tgggaaaaat ccctcggcac attgaagggg ctggtcgaaa tccgacgttt ggaaaacaac
      661 gatgccatgc tgatttctcc cgaacaggca tattttgtgc gtgaaaacct gcgcctccgc
      721 cttttggatg cgcgcactgc attaatgcag cgcaacagcg aagtctatca gggcgatttg
      781 aacaatgccg aagccgccgt cagacagtat ttcgatgcca agtctcccgc cacgcagtcg
      841 tggctgaaag aactggcgga attgaaggcg ttggatgtgc ggatgactgc ggatgacggt
      901 ttgaaaaaca gcctaaatgc cgtccgcgcc tatcgcgacg gtacgcgcat gacggcggcg
      961 gaaaatcaag aagcggaaca ggcggcttcc gaaccggcaa acgaaaaaac agcttccgaa
     1021 ccggctgccg tatcggatgt gaagaccata gaagcaccgt ccctgccttc ggaacgcaaa
     1081 ccggaacagc ctgcaaaaaa acagaccgta ccggaaaagg cagggcgttc gccgtccgct
     1141 aaaggagaac gcgcatgaaa acggtagtct ggattgtcgt cctgtttgcc gccgccgtcg
     1201 gactggcgct ggcttcgggc atttacaccg gcgacgtgta tatcgtactc ggacagacca
     1261 tgctcagaat caacctgcac gcctttgtgt taggttcgct gattgccgtc gtggtgtggt
     1321 atttcttgtt taaattcatt atcggcgtac tcaatatccc cgaaaagatg cagcgtttcg
     1381 gttcggcgcg taaaggccgc aaggccgcgc ttgccttgaa caaggcgggt ttggcgtatt
     1441 ttgaagggcg ttttgaaaag gcggaactag aagcctcacg cgtgttggtc aacaaagagg
     1501 ccggagacaa ccggactttg gcattgatgc tgggcgcgca cgccgccgga cagatggaaa
     1561 acatcgagct gcgcgaccgt tatcttgcgg aaatcgccaa actgccggaa aaacagcagc
     1621 tttcccgtta tcttttgttg gcggaatcgg cgttgaaccg gcgcgattac gaagcggcgg
     1681 aagccaatct tcatgcggcg gcgaagatga atgccaacct tacgcgcctc gtgcgtctgc
     1741 aacttcgtta cgctttcgac aggggcgacg cgttgcaggt tctggcaaaa accgaaaaac
     1801 tttccaaggc gggcgcgt
//
LOCUS       DAWHRA010000205         1740 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWHRA010000205 DAWHRA010000000
VERSION     DAWHRA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.205"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            95..901
                     /locus_tag="WIB30_002031"
     CDS             95..901
                     /locus_tag="WIB30_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ3798030.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            complement(959..>1740)
                     /locus_tag="WIB30_002032"
     CDS             complement(959..>1740)
                     /locus_tag="WIB30_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ3798031.1"
                     /translation="VDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQA
                     LEKLGKDVKGATYAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALF
                     KEFQEKGFITNEAGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGA
                     NGPTTPEADVILRQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVV
                     LRRAFRDIWNLAQEYDVDLRTASYMMSIRRVEKAMKLRGWY"
BASE COUNT          474 a          528 c          377 g          361 t
ORIGIN      
        1 agacagtttt cagacagcaa atccatcttc acacgatacc tattttgtta taacataaca
       61 aaatctttaa cccacacgag acaaaggctg caccatgaag aaaacattga cactgctcgc
      121 cgtttccgcc ctatttgcca catccgccca cgcccaccgc gtctgggtcg aaaccgccca
      181 cacgcacggc ggcgaatacc ttaaagccga cttgggctac ggcgaatttc ccgaactcga
      241 acccatcgcc aaagaccgcc tgcacatctt cagcaaaccg atgcagctgg ttaccgaaaa
      301 aggcaaggaa aacatgattc aacgcggcac atacaactac cagtaccgaa gcaaccgtcc
      361 cgttaaggac ggcagttacc tcgtcatcgc cgaatatcag cctactttct ggtcaaaaaa
      421 caaagcaggc tggaaacagg cgggcatcaa agaaatgcct gacgcaagct attgcgaaca
      481 aacccgaatg ttcggcaaaa acatcgtcaa cgtcggacac gaaagcgcgg acaccgccat
      541 catcaccaaa ccggtcggac aaaacttgga aatcgtcccg ctggacaatc ccgccaacat
      601 tcacgtaggc gaacgcttca aagtccgcgt tctgttccgt ggcgaaccgc tgcccaatgc
      661 caccgttacc gccacctttg acggcttcga caccagcgac cgcagcaaaa cgcacaaaac
      721 cgaagcacag gctttctccg acagcacaga cgacaaaggc gaagtggaca tcatcccctt
      781 gcgccaaggc ttctggaaag ccaatgtcga acacaaagcc gacttccccg atcaaagcgt
      841 gtgccaaaaa caggcgaact actcgacttt aaccttccaa atcggccatt cgcaccatta
      901 atcccgcccg cacaaaaatg ccgtctgaag gcttcagacg gcattttttg ttcaaacatc
      961 aataccaacc gcgcagcttc atcgcttttt caacacggcg gatactcatc atgtaagacg
     1021 cggttcgcaa atcgacatcg tattcttgag ccaagttcca tatatcgcgg aacgcgcgtc
     1081 gcaggacgac ggtttctttc tcttgaactt cgtcaaactc ccaataatag ccttgcaggt
     1141 tttgtaccca ttcaaaataa gataccacca cgccgccgca gttcgccaaa atatcaggca
     1201 cgaccaatac gccgttttga cgcaggataa cgtcggcttc gggcgtagtc gggccgttcg
     1261 cgccttcgac cacgattttc gcgcggactt taccggcgtt ttcggaagtc agttggtttt
     1321 ccagcgcgca aggggcgagt acgtccacat ccaaagccaa aagttcggcg ttggtaattt
     1381 ctttgccgta accggcttcg ttggtgatga agcctttttc ttggaactct ttaaacaaag
     1441 cttccatatc caagccgttt tcgttgtaaa tggcaacatc gacagtagaa accgcaacaa
     1501 ctttcgcgcc ggattgatgc gcgtaataac ctgtgtggta acccacatta ccgaaacctt
     1561 gaatggcgta agtggcaccc ttcacgtcct tgcccagttt ttccaaagct tggacggcgg
     1621 cgaggttcac gccgtaaccg gtagcctcgg tacgcgccaa agagccgccg aactcaaccg
     1681 gttttccggt aaatacgccc ggcgcggaat gtttcaccac gttttcataa gcatccacca
//
LOCUS       DAWHRA010000206         1726 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWHRA010000206 DAWHRA010000000
VERSION     DAWHRA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.206"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(61..840)
                     /locus_tag="WIB30_002033"
     CDS             complement(61..840)
                     /locus_tag="WIB30_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ3798032.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            1343..1648
                     /locus_tag="WIB30_002034"
     CDS             1343..1648
                     /locus_tag="WIB30_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ3798033.1"
                     /translation="METWVQNYTAIGGSLYLTAAAALLPIVFFFAALTVLKLKGYQAG
                     LYTLLIALAVAVFGFGMPAVWRFLPCRSSRTDPTPLRHAGFDRALQNRQIHPHRFGL"
BASE COUNT          434 a          518 c          353 g          421 t
ORIGIN      
        1 ggtttaaatt tagttcacta taccgataca tccgaatatg ccagaccgtc taaacggtat
       61 tcatacctcc caatcctgca cgcgcttcaa atcgttttgc gccaaagtat ccgcgtggcg
      121 gttacggctc tgatattccc tgtctttcat gatgctgctt gccacataat tcaaatgtgc
      181 ctttgccgcc tccgaagcct cgcccggccg acggttcgat atcgcctcat acagcacgcg
      241 gtgctgcgcc atcagtttcg gacgcggatc ttcttcctga ttcagataaa taaggctgct
      301 gcgcgtctgc cggtacagca ttttcaacaa accgcccgac aaatggctga acaacaaatt
      361 gtgcgccgca tccgcaatcg tctgatggaa gctgacatcg gcttcgctct gatgttctag
      421 attgccgctt tcgcacgcct cctcaaactt ttcaagccaa aacccaatcc gcttcaaatc
      481 ggcatccgtc cggcgttctg ccgccaatgc cgccatacag ccctcgatgt ggcagctgaa
      541 atcaaaaaca tcctgttccc aattggaatg cttgcccaaa agctcctgcc aactttgcaa
      601 aaaatcctgc tgcggcttga ccgaaacata ataaccgtct ccctgcctcg cttccaaaac
      661 ctgacgggcg accaaaacat tcaatgccga cctgaccgac gggcgcgaaa cgccgaactc
      721 ttccgccaaa acgcgttcgg gcggaatctt gcccccttcc gcgtaaaccc cttccgcaat
      781 gcgctcctcc aataccgaca atacctgatc gctgattttc tgaggcctta ccagtttcat
      841 cactcctcct ttataaagat tccctgcaga acccttccga aatatagtgg attaacaaaa
      901 atcaggacaa ggtgacgaag ccgcagacag tacaaatagt acagaaccga ttcacttggt
      961 gcttcagcac cttaaagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtt
     1021 tttgttcatc cactatacat caaacatcaa attggactga ccaatcaggg cggattctaa
     1081 tgacacgccg ttcccgccgt caacggcatt tacctcgcac cgcccccgaa acacaagaaa
     1141 aaactacacc aaactacaat ttttgttcat gcaaatattt gttttgacag gatttaaacg
     1201 aaagctccga ttcaaatctg ccgaaccgcc caaaaatata ttgacctaaa tattaaagtt
     1261 tcgtaaagta atgcaacgtt gctttaattg gtttgaccac tattgccgac gattagaaaa
     1321 atattttcgg agatgttcaa ttatggaaac ttgggttcaa aactacacgg caatcggcgg
     1381 cagcctgtat ctgactgccg ccgccgcact cttacccatc gtctttttct ttgccgcgct
     1441 gaccgtcctg aagctgaaag gctatcaggc ggggctttat acgctgctga ttgcgcttgc
     1501 cgttgccgta ttcggcttcg ggatgccggc ggtatggcgg tttcttccct gccgcagcag
     1561 ccgcactgac ccaacgcccc tacgccacgc tggatttgac cgcgcattac aaaatcggca
     1621 aatccacccg catcggtttg gactttgaaa acgtgttcaa caaacgctac cgccctatgc
     1681 ccgacattca cgtttacggc acgccgcgca gcctgaccgc aaccgt
//
LOCUS       DAWHRA010000207         1687 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWHRA010000207 DAWHRA010000000
VERSION     DAWHRA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.207"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..94
                     /locus_tag="WIB30_002035"
     CDS             <1..94
                     /locus_tag="WIB30_002035"
                     /inference="COORDINATES: protein motif:HMM:NF014825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HEZ3798034.1"
                     /translation="LEARGQIVHRNDPCPCGSGLKYKQCHGKLA"
     gene            109..261
                     /locus_tag="WIB30_002036"
     CDS             109..261
                     /locus_tag="WIB30_002036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798035.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            239..>1687
                     /locus_tag="WIB30_002037"
     CDS             239..>1687
                     /locus_tag="WIB30_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ3798036.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQLYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITASALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALD"
BASE COUNT          414 a          452 c          461 g          360 t
ORIGIN      
        1 cttggaagcg cggggacaaa tcgtccaccg caacgacccc tgcccctgcg gcagcggttt
       61 gaaatacaaa caatgccacg gcaaactggc ttaagcgttt gaacgcaaat gccgtctgaa
      121 catcccgctc ccgtttcaga cggcattttg cctgaaccgc cacatccgac tgccattccg
      181 aaaaatcccg atttcgtacc gtccgtacca aaaacagaca tcccgtccgc cccacatcat
      241 gattccatcc gacttcattg acgagctttt agccaaaacc gatattgtcg atattatcga
      301 cgagcaggtt ccgctgaaaa aaggcggggc gaactatatg gcgtgttgcc cgttccacaa
      361 ggaaaaaacg ccgtcgtttt cggtcagtcc gaccaaacag ttttaccact gtttcagttg
      421 cggggcgcac ggttcggcga ttggttttgt gatggaacat caggggctgt cgtttccgga
      481 ggcggtgcag ttccttgccg accgcgtggg tatgattgtg cctaaagtgc gcgggcaaaa
      541 cgataatccc gaagtccgtg ccgagcgcaa gaaaaaacag cagacgctgg aggaaacgac
      601 ggctgcggca gctgattttt atgcgcaaca gctaaaattc aatccagcgg caaaagccta
      661 tttggacaag cgcggcttga gcgcggaagt catcgcgcat tatggtttgg gctacgcgcc
      721 cgacggctgg cagcctttgg cgcaagtgtt ccaactatat ccgaataccg cgttggtgga
      781 tacgggtatg gtgattgaca atgaggggcg gtattacgac cgattccgtc atcggattat
      841 gttccccatc cgcaatccgc gcgggcaggt aattggtttc ggcggcaggg tgctggacga
      901 ctccaaaccc aaatatctaa actctcccga tacacctttg tttgacaaag ggaaaaacct
      961 ttacggcttg tatgaggggc gtgccgctgt caaggaagcg gagcggattc tggtggtcga
     1021 aggctatatg gacgtggtcg cgctggcaca gttcggcgtg ggctacggtg tggcggcctt
     1081 gggcacggca acaacagctg aacacgtcaa aatcctaatg cgtcaggcgg acagtattta
     1141 tttctgtttc gacggtgaca gcgcggggcg caaagcggct tggcgcgcgc tggaaaacgc
     1201 gctgccgcaa ttgaaggacg acaaatcgct gcattttttg ttcctgccgg aagaacacga
     1261 ccccgacagc tacatccgcg cctacggcaa agcgcaattt gaagacgcgc tgttgaatca
     1321 aagcaagccc ttgtcggaat atttctggga acacctttca gacggcattc atctcaatac
     1381 gcaggaaggc aaggcggaat tggtaaaaac cagttcgccg cttttggcgc agattaccgc
     1441 gtcggcattg gcttatttgt taaaacaacg gcttagcgaa ctggtcggca tcgaccccga
     1501 caacctcgcg caactgctgg gacaggaagc accgaagcgg cacgtcaaac aaaaaaacta
     1561 caaactgccg ccgatttccg tcaaacagcc cgttatgccg acattggtgc aacggcaaat
     1621 ccgcagcctc ttgataaatc cggattgggc tgcatatata gacctgcccg attatctggc
     1681 gttggac
//
LOCUS       DAWHRA010000208         1660 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWHRA010000208 DAWHRA010000000
VERSION     DAWHRA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.208"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            306..1466
                     /locus_tag="WIB30_002038"
     CDS             306..1466
                     /locus_tag="WIB30_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ3798037.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     QNIQQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGATRW
                     GNRESFVGLAGEFGTLRAGRVANQFDDASKAIDPWDSNNVVASQLGIFKRHDDMPVSV
                     RYDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQQTPQSQPTLVPAVVGKPGSDVYYAG
                     LNYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTGGYE
                     EGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKG
                     ENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          415 a          395 c          423 g          427 t
ORIGIN      
        1 atccactata aagtgccgtg tgtgtttttt atggcgtttt aaaaagccgg gactgcatcc
       61 gggcagcaga gcatcggccc gcacgaggtc tgcgcttgaa ttgtgttgta gaaacacaac
      121 gttttttgaa aaataagcta ttgttttata tcaaaatata atcattttta aaataaaggt
      181 tgcggcattt atcagatatt tgttctgaaa aatggttttt tgcggggggg gggtataatt
      241 gatgacatat cgggtgtttg cccgatgttt ttaggttttt atcaaattta caaaaggaag
      301 ccgatatgcg aaaaaaactt accgcgctcg tattgtccgc actgccgctt gcggccgttg
      361 ccgatgttag cctgtacggc gaaatcaaag ccggcgtgga aggcaggaac atccagctgc
      421 agttgaccga accgctccaa aatattcaac aacctcaggt tactaagcgc aaaagccgca
      481 tcaggacgaa aatcagcgat ttcggctcgt ttatcggctt taaggggagc gaggatttgg
      541 gcgaagggct gaaggctgtt tggcagcttg agcaagacgt atccgttgcc ggcggcggcg
      601 cgacccgttg gggcaacagg gaatcctttg tcggcttggc aggtgaattc ggcacgctgc
      661 gcgccggccg cgttgcgaat cagtttgacg atgccagcaa agccattgat ccttgggaca
      721 gcaataatgt tgtggcttcg caattgggta ttttcaaacg ccacgacgat atgccggttt
      781 ccgtacgcta cgattccccc gaattttccg gtttcagcgg cagcgtccaa ttcgttccgg
      841 ctcaaaacag caagtccgcc tatacgccgg ctcattttgt tcagcagact cctcaaagtc
      901 agcctactct cgttccggct gttgtcggca agccggggtc ggatgtgtat tatgccggtc
      961 tgaattacaa aaatggcggt tttgccggga actatgcctt taaatacgcg aaacacgcca
     1021 atgtgggccg tgatgctttt gagttgttct tgctcggcag cgggagtgat gaagccaaag
     1081 gtaccgatcc cttgaaaaac catcaggtac accgcctgac gggcggctat gaggaaggcg
     1141 gcttgaatct cgccttggcg gctcagttgg atttgtctga aaatgccgac aaaaccaaaa
     1201 acagtacgac cgaaattgcc gccactgctt cctaccgctt cggtaatgca gttccacgca
     1261 tcagctatgc ccatggtttc gactttatcg aacgcggtaa aaaaggcgaa aataccagct
     1321 acgatcaaat catcgccggc gttgattatg atttttccaa acgcacttcc gccatcgtgt
     1381 ctggcgcttg gctgaaacgc aataccggca tcggcaacta cactcaaatt aatgccgcct
     1441 ccgtcggttt gcgccacaaa ttctaaatat cggggcggtg aagcggatag ctttgttttt
     1501 gacggctcgc cttcattctt tgattgcaat ctgactgcca atctgcttca gccccaaaca
     1561 aaaatccgga tacggaagaa aaacggcaat aaagacagca aataccgtct gaaagatttt
     1621 cagacggtat ttcgcatttt tggcttggtt tgcacatata
//
LOCUS       DAWHRA010000209         1648 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWHRA010000209 DAWHRA010000000
VERSION     DAWHRA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.209"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1627)
                     /locus_tag="WIB30_002039"
     CDS             complement(<1..1627)
                     /locus_tag="WIB30_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ3798038.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEVNAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWNERQLTQSGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLRVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLITAAAFVR"
BASE COUNT          346 a          517 c          432 g          353 t
ORIGIN      
        1 cgcgcacgaa ggcggcggca gtaatcagtc cgccgttgct gccgccctgc aagccgatgt
       61 gttcgggcga actgataccg cgttcggaca aatcgcgcac gactgccaat aaatcatcaa
      121 cgcttttatg tttgctgatt ccctgcgccg cctgatgcca gcgcgggccg aactcgccgc
      181 cgccgcggat gttcgccaat acaaaggcat tgccctcttc cagccaatat ttgccaatgc
      241 tgcccagata atgcggcaat tcgggaatgc cgaaaccgcc gtaggcatag accagcgtcg
      301 gcatgtcggg cgcggcgttt ttgccgacgt ggaaataagg aatgcgctcg ccgtcagccg
      361 aagtcgtcca aaactgctgc acgttaatgc cgtctgaatc aaactgctgc ggctggcggc
      421 gcatgacggt cagttccatc acgttcaaat ccagcgcaaa cagcgtcagc ggcgtggtga
      481 aatcgctggc ggcaaggtaa accacgtcgc cgccccaagg ttggtcggtc atttccaacg
      541 cgcccgaagg caggcgcggc aattcgactt cctgccattt gccgtcggca aaccgccatg
      601 ctttcagacg gccttgtacg ttctccaaca ggctcgccac cacaaaacgc ttggtcgttt
      661 ccacgctttc caatgcctgc gtttcatcgg gcgcaaacaa aagctgcgcc gccccgagtt
      721 cgccccgatt cagcttcacc gccaccagcg cgccgctcgg atagctttgg ttcgcgcggt
      781 tccagtcctt gcgcagcgtc agcaaaagat gccccgccag atagccgacc acgtcgcaat
      841 cgttgggcag gtttaacggt ttcgcctcgc cttcggctga gacccgcaaa taggttttgg
      901 tgtaaaaacc gtccgacgct tcaatcaaat caatcggcga accctgcgga tcgagataac
      961 gccacgcgtt caccatcatg ccgtcttcgc cgatttgata cacaggcagg ctttcctcga
     1021 aactcttgcc gcgttccacc agccatactt cgcgcggata gcccgattgg gtcaactggc
     1081 gttcgttcca agccggacac acccacacgc tgttttcatc gcgccacgac acatggtttt
     1141 tgcctgccgg aaagtgaaaa ccgccttcta ccaattcccc tgcttccaaa tccacttcca
     1201 gcgtgtacgc cgtatcaccg cccgacttgc tcagtgttaa caacgcgcgg ttgggctttt
     1261 ccaccaagtg cgatacgccg cccaaataca catcatcgcc gagcaattca tcgaaatccg
     1321 ccactgaaaa caggattttc cactcgggat agccggaacg gtaagtcgcc gccgtacaca
     1381 cgcggtacac gcccttcgga tattccgcgt cctgatggaa atggtacatc cgcgcgcggt
     1441 gttcctgaca aaacggaatc tgccgcgtgt cctgcatttg attcaaaatg ccgtctgaaa
     1501 gcgcgcgcgc cttgtcgttg tttaaaaaac gcgcgcgcgt ttcggcattc acttcagcag
     1561 cgaagttttg cgtttcggcg gaatcgaggt tttcaaaatg gcggtagggg tcggggtagg
     1621 atttcatcgg ggtccttgag ggttgggc
//
LOCUS       DAWHRA010000210         1644 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWHRA010000210 DAWHRA010000000
VERSION     DAWHRA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.210"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(18..680)
                     /locus_tag="WIB30_002040"
     CDS             complement(18..680)
                     /locus_tag="WIB30_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3798039.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHTDYTRWRAEYLDEYDSRYAQDTTLFGGVNELIAELGKRG
                     IKWGIITNKPMRFTDKLVPKLGFIVPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QS"
     gene            complement(719..1546)
                     /locus_tag="WIB30_002041"
     CDS             complement(719..1546)
                     /locus_tag="WIB30_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ3798040.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
BASE COUNT          311 a          435 c          506 g          392 t
ORIGIN      
        1 tgtggggcgg atatttttca ggactgtatt ttgtccagac attcgagcag atcgagcggc
       61 gtgcggatgt ggaaatccgc ctgccatgag ccggtatcgt cttcgggagc gatgtagccc
      121 cattcggcga ggacggtttt catgccggcg ttgcggcctg cttggatgtc gcgttcggca
      181 tcaccgacgt agagtgtgtg ttgcgggtcg gcgtggattt gtccgcacgc atacagcatg
      241 ggtttgacgc tgggcttggg ttcgccgcag gtgtcgccgc tgacgacgac ggcgggtggg
      301 acgatgaagc cgagtttggg gacgagtttg tcggtgaagc gcatgggttt gttggtgatg
      361 atgccccatt tgatgccgcg ttttccgagt tcggcgatga gttcgtttac gccgccgaag
      421 agggtggtgt cttgggcgta gcggctgtcg tattcgtcta ggtattcggc gcgccatcgg
      481 gtgtagtcgg tgtggtcggg ggtgatgcct gcgccgagtt tgatcagtcc tgccgcgccg
      541 tggctggctt gggtgcggat ttcgtccatg ctttttgcag gtagtccgtg gcgggcgagc
      601 agggtgttga gtgcgccgcc gaggtctagg gcggtgtcgg caagcgtgcc gtcgaggtcg
      661 aacaatacgg cttgtatcat gtgttttcct tttttataaa gtgcgggact gaagggtttc
      721 agacggcatg tttattttgt ttcaaaccct gctcgaaatc ttccaacata tccaattcaa
      781 agcggctgaa gcctgctttt tcgcgcgctt cgatgttcac atagccccgg aagataaaca
      841 tatcgtaacg ggcaatcaga ctgcggaaca gggtaatcgg ttccaaaccg cgttcgcggc
      901 agaggtgttg ataccaatgg ttgccgatgg cgacatgacc cacttcgtcg cggtaaatga
      961 tgtccaatac gccgcaggtt tccgaatcgc cgcgctgcgc caccttcgcg cgtatccccg
     1021 gcgtaacgtc cagcccgcgc gcttccaaca cgcgcggtac caacgccata cgcaacagcg
     1081 ggtcgtaggc ggttttgtac gccatatccc acaaatggtt gtgcgcctcg aaactgccgt
     1141 aatcgaagcc gaaagcgcgc aatctgtcgc gcatcaggcg gaaatggtac acctcttcct
     1201 tcgccacttt cacccagtcg cggacaaact gaaacggcag cgtgcggaaa cggtatgccg
     1261 cgtccaaagc tagattgacg gcgttgaatt cgatatgcgc aatcgcgtgc agcatcgccg
     1321 cataaccttc ggttgtgttc attttgcgcg gggtcagttg cgacggcgcg accaaaacag
     1381 gcttgtccgg ccgtcccgct cgggggaagt ccgccggagg cgcgtttgct tccgccccgt
     1441 ccgcattttg aacggcggca aacgcctcat ccgtcagccg tcctttttca tctgggtcgc
     1501 ccgaaagcag ggcgcgttcc agcaaagcat aaatatcgag tttcatctca agtccgccgt
     1561 gttcggaaaa caaatattat agcgttttaa aaaaacaaga tgaggcatat aatctccgcg
     1621 attcggcatt ccgcgcccaa accg
//
LOCUS       DAWHRA010000211         1642 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWHRA010000211 DAWHRA010000000
VERSION     DAWHRA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.211"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1120)
                     /locus_tag="WIB30_002042"
     CDS             complement(<1..1120)
                     /locus_tag="WIB30_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ3798041.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSETIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKK
                     LNLTFEHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKR"
BASE COUNT          500 a          396 c          252 g          494 t
ORIGIN      
        1 gccttttttc cctatcgaat ttgtcagaat ttttaaaagc aataaccccc aattcatcaa
       61 gttcaatttc caaatcaata tccggctgtt catgaataaa accgtgtttc aaaccgtccc
      121 ttaacatttc cagagcatat ttttcaggtt ctaatccttt gggaaatccc gaatctgcct
      181 ttaaagattc cacaatttcg ctattatcaa aacccatttc aatcatgaaa ttgaccagtt
      241 ttccaaccgc gtttttcgcg taatgcaatt tatgctcgaa agttaaatta agctttttct
      301 ttctctgatc aaacctttcg ggaaccggca tatttttaaa ttttctacaa attggaaaag
      361 ctccacagaa atacgaaccc tgatttatta aaatatccaa gggtatttct atatctccat
      421 aattaaactg gatctcgaac cttacccatt tgctttcttt atctccaagc tgcctgcctt
      481 tctcataaac acgaacaaaa cgagaatttt tcttgcgacc tacataaaat gtcttgccgc
      541 tcccgtcctc attccgccaa gccgtaccga tcgtttcaga tttcggcctt tgattgctgt
      601 tatcaaaaaa accattatcg tgatctaaca acgcctgatc cggcgtgtac tctccatcaa
      661 aaaaatcaag tgctaggtca attcgcgtta ttcttgtcct tatcgaatca tcgagaaact
      721 gctttagcct caactcccaa cccggacttg caacgctgca accagtacct ttcaactcaa
      781 ctaaaacagt attgcgctga cctccgaaat gcacctctcc ataatcaaca tcatccgaac
      841 ctaacctata catggattca tagaatttgt tgccccttga tttgcatttg cgcgttatgc
      901 caaaacctag aatttcttcc agctttctgc ttaatacata catgtattca gcatcagaaa
      961 ataaagggca accggaaact ttcagtaaag tatcttcatg gaatgtgaat gaaatccaat
     1021 caacaaaaac cccgtcttgc ttacctcttc gctgcggaat ttctaaaagc ttcccttttc
     1081 cgtccgatat aaagtgtgag aaatattcaa cttcactcat tcgttcacta ctcttggaat
     1141 tggtttttaa tttcgctccc cccctgttag tcaagggggg gggctttcag ccgtttcccg
     1201 tcttccgcgc taaagcgcgt acgacggtca acgaccgaaa gccaaatcct gacaaacttt
     1261 taaagatcaa caaaaaagac cacaaccatc cgttgtgata atcactggaa aattcgaacc
     1321 aaccgaatct atataatcga acgcctgata aagctttgaa aaattctctt gattaatgag
     1381 tttatgcggt tcgccatgtc tgaactgata aaaacacaaa acacaagaat ctgatttttt
     1441 aaatattctc caataagaac aggaaaatat tacatttact gctgacataa aaaagcccct
     1501 ttcacttggc tgtcaaaggg gaatgttaag aaaattaatg cgcccctttg atagagcgca
     1561 tcatataagc gcatcatata aggcgggaat ctaggtctgt cagtgcggaa acttatcagg
     1621 taaaacggtt tcttgagatt tt
//
LOCUS       DAWHRA010000212         1635 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWHRA010000212 DAWHRA010000000
VERSION     DAWHRA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.212"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            261..1565
                     /locus_tag="WIB30_002043"
     CDS             261..1565
                     /locus_tag="WIB30_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ3798042.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          313 a          423 c          505 g          394 t
ORIGIN      
        1 caaacctctg tatattttcc tacagtaaat tgttgacaat ccatacgccc acatatgcgc
       61 cgcctaagga taaatcctcc cgccggacaa cgggtgcaag ggatcggatg cgatatttcc
      121 atattcaaac aagggattgg cggcacatcg gcaaaatccc cgcgccgccc cggtccggca
      181 gggcttgcgt ccctcccgga caagccccga ccccgccttt ccgaaagacg ggctcaacca
      241 ttaaggaaac tttaatcaaa atgaaaaaac acatatgggc ggcatctttg ctgccggcat
      301 ccctatcggc agaaccttta aactggtgga agccttattc cgccgtcaat tcgggcgata
      361 ccgcctgggt gatgactgcg gctgccttgg tactgttgat gacgcttccc gggctggctt
      421 tattctacgg cggtatggtg cggaaaaaaa acctgctctc gacgatgatg cacagctttt
      481 ccatcgcgac attggtgggc atcctttggg tcgccgtcgg ctattcttta gcgttcacgc
      541 cgggaaatgc ctttatcggc ggtttggggc gcgtattttt aagcgggatg cagatagacg
      601 ctaccgcaca gatgctgacc gtgtcgccca atgcgccgac tgttcccgaa ccggtattta
      661 tgttttttca gatgacgttt gccattattt cgaccgccat tattaccggc gcgtttgccg
      721 aacggatgaa atattcggca atgatgctgt tttcgggcat atggttttta ttggtttatg
      781 tgccgggcgc gcattgggtg tggggcggcg gctttatgag caagggcggc gtattggatt
      841 atgccggcgg tacggtggtg cacatcaatg ccggtatcgc gggactcgtc gccgccttgg
      901 ttttgggcag gcgcataggc tacgggcgcg aggcgatgcc tccgcacaat atggcgatga
      961 cactgatcgg cgcggcaatg ttgtggttcg gctggttcgg ctttaacgcc ggatcggcgc
     1021 ttgcggcaga cgcggcggcg ggtatggcga tggcggtaac gcaggtgtcg gccgtattcg
     1081 gcgcggcagg ctggcttgcc tgcgaaaaaa tagcgggaca caaaccttcc gctttggggc
     1141 tggcttccgg cgcggtttcc ggtctggtcg gcatcacccc tgccgccggc tttaccggcc
     1201 cgtcgggcgc ggccgccatc ggtatattga ctgccgccgc gtgctttgtg tccgtcaccg
     1261 tcgtcaaaca caaattgcgt tacgatgatt ctttggacgc tttcggcata cacggattcg
     1321 gcgggctggt gggcggaata ttgaccggca tctttttcga caaccgcatt ttcggcgggg
     1381 atgcggcagt ttggcagcag ttgtggatac aggtaaaaga cgggttcgtt atggcggcat
     1441 acagcgggct gatgagttgg gcgattttga aggtcgtggg gaaaatctgc ggcggcctgc
     1501 gcgtcggcaa ggatgtcgaa cgcgaaggtt tggatctgaa tatccacggc gaacgcgtgg
     1561 aataagggcg gctatgccgt ctgaagcctg aaaatcgggt ttcagacggc atttttcacg
     1621 tttgccgccg atgga
//
LOCUS       DAWHRA010000213         1635 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWHRA010000213 DAWHRA010000000
VERSION     DAWHRA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.213"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..380)
                     /locus_tag="WIB30_002044"
     CDS             complement(<1..380)
                     /locus_tag="WIB30_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798043.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WIB30_002045"
     CDS             complement(438..803)
                     /locus_tag="WIB30_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798044.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="WIB30_002046"
     CDS             complement(785..1330)
                     /locus_tag="WIB30_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798045.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
BASE COUNT          509 a          360 c          253 g          513 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtgt ggaacacttg gtcttcaaat gcctgattgt
     1261 ccaagccgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtgattt
     1321 gttttttcat aataactcct ttggattaca aggttggaaa atcaaagccc tgcttagaac
     1381 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
     1441 tgtcattgtt ccgtgcaaaa gggattttta ttgatgagtt gtgtactggg tttcagcttg
     1501 gctttttaga taatcttttc acaagaacat acatcaatac tgtcccaaaa aaaagatttg
     1561 ataaatatat tgcaagatat tttgttccat cttcatccga aacagaaacc aatatatttt
     1621 taattaatac atagc
//
LOCUS       DAWHRA010000214         1615 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWHRA010000214 DAWHRA010000000
VERSION     DAWHRA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.214"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(308..409)
                     /locus_tag="WIB30_002047"
     CDS             complement(308..409)
                     /locus_tag="WIB30_002047"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ3798046.1"
                     /translation="MHEYIRYYNNDRIKLKLKGLSPVQYRTQSLKAA"
     gene            complement(416..526)
                     /locus_tag="WIB30_002048"
     CDS             complement(416..526)
                     /locus_tag="WIB30_002048"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ3798047.1"
                     /translation="MSRKGNCLDNAAMESFFGTLKSECFHTCKYDSVTES"
     gene            complement(550..1083)
                     /locus_tag="WIB30_002049"
     CDS             complement(550..1083)
                     /locus_tag="WIB30_002049"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ3798048.1"
                     /translation="MSFYYQLAVQSAEDKYADLKRHIHDIYRRHKGRYGYRRIAAAIR
                     HAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEKPNEKWV
                     TDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPGSSEKPI
                     LHSDQGWQYQMFFIKSS"
     gene            complement(1116..1526)
                     /locus_tag="WIB30_002050"
     CDS             complement(1116..1526)
                     /locus_tag="WIB30_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ3798049.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAKQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
BASE COUNT          381 a          413 c          344 g          477 t
ORIGIN      
        1 tcagcagcat cctgcaacac aaaaaagcct tgaacttcaa cagtttcaag gcttttttgt
       61 gttgcaggat gctggcgtat gcttgagttt tcgccattta tggggtaact tttggggtaa
      121 cttttcacga attaccccaa aaaacgcctt aaaaatgcaa aagtcatcac gcattttttt
      181 aaaaaggtta cccaaaactt aaatgtaatc ctttatttat aagcttgcag ctttccaaac
      241 aagttacccc caaaacggat aaaaaaatgc cgaagccgaa ctgaccccaa aaagtcggac
      301 agtttaatca agcggctttc agggactgag ttctgtactg aacagggctc agtcctttta
      361 atttcaactt gattctatcg ttgttgtagt aacggatata ttcgtgcagt gccgcttacg
      421 attcggtaac ggaatcatat ttgcacgtat ggaaacattc cgatttcaac gttccgaaga
      481 aactttccat tgccgcattg tccaagcagt ttcccttgcg ggacatactc tgaaccagac
      541 cgttgccttt caactgcttt tgataaaaaa catctgatat tgccaacctt gatccgaatg
      601 cagtatcggc ttttcagacg atcccggttt ctccggcgca cctttcagta tctcgccggc
      661 caaatcgaaa gtcgggcgga tttgaatacg gtaactgacg atttccccgt taaacaaatc
      721 cataatcgga gaaaggtata tcttttctcc gcctacattg aactcggtaa cgtccgttac
      781 ccatttctca ttcggctttt ctgcatggaa acagcgtcgc aggatattcg gcgcaatttt
      841 gccgacttct cctttgaacg agcggtattt gcgccgccgt atcactgcct tcagccccgt
      901 cttcgccatc agacggctga ctttcttgtg attgaccggt gttcctgcgt gacggatggc
      961 tgccgcaatc ctccggtagc cgtatcttcc cttatgtcgt cgataaatat catggatatg
     1021 ccgtttcaaa tcggcatatt tgtcttctgc cgattggacg gccaattggt aatagaagga
     1081 caatctccaa cagcagttca acggatggca ttgccttaac cctgcgacga gttgcgttct
     1141 ttctgccgca cttctttccc atggattaag gcatcgggct tttttagggc aaccatttcc
     1201 gctttaaggc aagccaattc cgcaagcagt tcttccttgg ttttcagata gccggctttt
     1261 ttcgtttgcg gcggatactg ttttttcacg ggctttcttc cttgtttttg ggaaaagtcg
     1321 taccaccgct gcctttcgtc ctgcgctttt ttaagctgca aggcatattg ttccgccgca
     1381 accatagaag aatgccgata ggtggcaatc agggcggttt gtttcgcttc ggccttgtcg
     1441 gcggcgtatc ggtaaccgca cgtccacaca agcaaaagca caaatgccca aagcagccgt
     1501 tttttggctc ggacaaattc aatcattgcc gccccccttc ctgatttgcg gattttttga
     1561 tttctgccaa ctgcggaatc gccgcgatgc cgcgtttgat taatgcatac ccgcc
//
LOCUS       DAWHRA010000215         1612 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWHRA010000215 DAWHRA010000000
VERSION     DAWHRA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.215"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(148..1191)
                     /locus_tag="WIB30_002051"
     CDS             complement(148..1191)
                     /locus_tag="WIB30_002051"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ3798050.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(1188..1469)
                     /locus_tag="WIB30_002052"
     CDS             complement(1188..1469)
                     /locus_tag="WIB30_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798051.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSTANQNPVGQRSDGIPANHTKEKP"
BASE COUNT          339 a          415 c          508 g          350 t
ORIGIN      
        1 attcccgctt ttgcgggaat gacggtattt tagatttttg ttttcgcgga ataatgagcc
       61 aaaagccgtc cgaacccaaa aaaaccgccg ctttcagatg gcatcaaaaa accgcaggca
      121 cgatgcctgc ggtttgccgt ttgcgtttca ggggagcagg gggattgcgt ccaagccgtg
      181 tgtttccttc agtccgaaca tgatgttcat attttgcacc gcctgtcccg ccgcgccttt
      241 gacgaggttg tcgatgacgg aaaggacgac ccacacatcg gattgcgccg cctgttggat
      301 gctgatgcgg cagaggtttg cgccgcgcac gctgcgggtt tcgggtgtcg aaccggcggg
      361 caggatgtcc acgaacaggc tgtcgcggta atagtcgcgc aggatggttt cggggcagat
      421 gccgtctgaa aggtggaggt aaacggtggc gtgcataccg cgtatcatcg gcgcgaggtg
      481 cggcgtgaac acgaatcctt cggcgatgcc gtcctgaagc ccggcgatgg tctgcctgat
      541 ttcgggcagg tggcggtgtc cagctatgcc gtaggctttg aagttgtcgc cgacttcgca
      601 caacagcgaa ccgacattgc ctttcctgcc cgcgccggac acgccggatt tgcagtcggc
      661 aatcagcggc ataccgggct tcagacggca ttgccgcaac agcggcacga gcggcaggga
      721 tacgcaggtc gggtagcagc cggggttggc gacgaggcgt gcctgtgcga cggcttcgcg
      781 gttgagttcg ctcaatccgt acacggcttg ggaaacgagg tcgggggcgg cgtgggtcat
      841 gccgtaccag tgttcccagg tcggaatgtc ccgtatgcgg aagtcggcgg aaaggtcgat
      901 gacgcgcacg ccctgttcaa tcaggcgcgg cgcgtctttc atggcgatgc cgttgggcgt
      961 ggcgaagaag acgatgtcgc attgttccaa acctgcctcg tcgggcgttt ggaaggcgag
     1021 gccgtacacg ccgcgcaaac tcggaaagta atcggcaact gcagttcccg cttcgctgcg
     1081 gctggttacg gcggcgactt cgacatcggg atgggcggca agcaggcgca gcagttccac
     1141 gcccgtgtag cccgtcgcgc cgacaatgcc gactttgatt tttttactca tggtttttcc
     1201 tttgtgtggt tggcgggtat gccgtccgaa cgctgtccga cggggttttg attggcggtg
     1261 gaaggacggt acacaaagcg tttgaaaagc gtgtctaatt taatggcatc cgcctctgtt
     1321 tttaaaaccc agccctgccg tccggaataa acataaccgt aggcgcgaaa gtcggcagga
     1381 tatttcggtg cctgccccaa acccaaaccg tatgcggcga aagcggtggt cgtggagaag
     1441 gaaagcagga ggaggacgag ggttttcata aggttccaaa ccggacgggg cgatgaagcc
     1501 cgattatagc aaaaggtcgt ctgaaaaccg ttttcagacg accttttatt taattacaat
     1561 aaaaatgtta acaacaaaaa acaaaccgct ttttttcgtt tggaaacaaa ac
//
LOCUS       DAWHRA010000216         1607 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWHRA010000216 DAWHRA010000000
VERSION     DAWHRA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.216"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            74..1447
                     /locus_tag="WIB30_002053"
     CDS             74..1447
                     /locus_tag="WIB30_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ3798052.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGREIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          420 a          527 c          386 g          274 t
ORIGIN      
        1 aatccactat atttccccgt cctatcggtt tcccgtttca gacgacataa ggtctgaaag
       61 aaagactaca attatgagta atccattttc ctctttaggt ttgggtacgg aactcgtttc
      121 cgcactgacc gcgcaaggtt acgaaaaccc gacgcccatc caagccgccg ccattcccaa
      181 agcactcgcc ggtcatgatt tgctagccgc cgcgcaaacc ggcacaggca aaaccgccgc
      241 ctttatgctg cccagtctgg aacgcctcaa acgttacgcc accgccagca cctcgcccgc
      301 gatgcacccc gtgcgtatgc tcgtcctcac ccccacgcgc gaacttgccg accaaatcga
      361 ccaaaacgtg cagggctaca tcaaaaacct gccgctgcgc cacaccgtct tgttcggcgg
      421 tatgaatatg gacaaacaga ccgccgacct gcgtgccggc cgcgaaatcg tcgtcgccac
      481 cgtcggacgg ctgctcgacc acgtgaaaca gaaaaacatc catttgaaca aagtcgaaat
      541 cgtcgttttg gacgaagccg accgtatgct ggatatgggt tttatcgacg acatccgcaa
      601 aatcatgcag atgctgcccc gccaacgcca aaccctgctc ttttccgcca ccttctccgc
      661 cccgatacgc aaactggcgc aagacttcat gaacgcgccc gaaaccgtcg aagtcgccgc
      721 gcaaaacacc accaacgcca acgtcgagca gcacatcatc gccgtcgata ccattcagaa
      781 gcgcaacctg ctcgaacggc tgattgtcga tttgcatatg aaccaggtca tcgtgttctg
      841 caaaaccaaa caaagcgtcg accgcgtaac gcgcgaactg gtgcgccgca acctgtccgc
      901 acaggcgata cacggcgacc gttcccaaca aagccggctc gaaacactca acgccttcaa
      961 agacggcaac ctgcgcgtcc tcgtcgccac cgacatcgcc gcgcgcgggc tggacattgc
     1021 cgaactgccc ttcgtcatca attacgaaat gcccgcccag cccgaagact acgtccaccg
     1081 catcgggcgc acggggcgcg cgggcgcgga cggcgtggcg atttccctga tggacgaatc
     1141 cgaacagaaa atgtttgaat ccattaaaga gctgaccggc aacaagctgc tcatcgagcg
     1201 catcgagggc ttcgagccgc aatggtggga acagggcggc gcaaaaccgg aaaaacccga
     1261 aatgcgcgaa ccgagacaac gcaaccgcta cgaatccgcc aaagcgcaac gcgaaaaaaa
     1321 cacccggccg gaaaatgcgg caaacgatgc gggcgcggct tgcggaaaaa ttgccggacg
     1381 cagccgccga agccgccggg aacaccggac gtgcgccctg ctccaaccgc gttacggcgt
     1441 aaaataaccc tgaaaatcaa atgccgtctg aacatttccc gtttcagacg gcatttttca
     1501 aaccagacta acgcattggg cgcaaccgcc cataccggat aaatttctgc cgcaaacagt
     1561 ttcagacggc atttgctgcc tgtacaatat agtggattaa caaaaat
//
LOCUS       DAWHRA010000217         1598 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWHRA010000217 DAWHRA010000000
VERSION     DAWHRA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.217"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(217..1140)
                     /locus_tag="WIB30_002054"
     CDS             complement(217..1140)
                     /locus_tag="WIB30_002054"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ3798053.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          431 a          383 c          385 g          399 t
ORIGIN      
        1 aaaacagaaa cctcaaatcc cgtcattccc gcgcaggcgg gaatccagac ttgtcggtgc
       61 ggacgcttat cggataaaac ggtttcttga gattccgcgt cctggattcc cactttcgcg
      121 ggaatgacga attttaggtt tctgttttgg ttttttgtcc ttgtaggaat gatgaaaatt
      181 taagttttag gaatttaccg gaaaaaatag aaagcgttat ccacaagttc tgatgttcag
      241 ctcgtgaaat gcgtcgggca aatcatcgct gtcggcaaat tccacccggt cgtaagccgt
      301 ttcgtctgcc aaaaccgcgc gcaagagtgc gttgttgatg gcgtgtcccg atttgtagcc
      361 ttcaaatgcg ccgacaatcg gatgtccgac gatatacaaa tcaccgatgg catcaaggat
      421 tttgtggcgc acaaactcat cgggatagcg caagccttca ggattcagga catccgtgtc
      481 gtcaatcacg atggcgttgt tcaaattgcc gcccaaaccc agattgtggg cgcgcatcat
      541 ttccacttcg tgcataaagc cgaaagtgcg cgcgcgcgcg atttcgtcga tgtaggattt
      601 gccggcgaaa tcgatttcaa aagtgggcga gctgcggttg aaaaccggat ggtcgaattc
      661 gatggtcagc gttaccttaa agccgtcata cggcgtaaag cgcacccatt tgcccgcttc
      721 tttgatttcg acaggcttga ggattttcaa aaaacgcttt tgcgcctttt gatcgaccac
      781 gcccgcatct tgcaaaaggt aaataaacgg caggctggag ccgtccataa tcgggatttc
      841 gggcgcgttc agctcaatca gcgcgttgtc gataccgtag gcggacagcg cggacataat
      901 gtgttcgatc gtgccgacac gcacgccttt gtcggtaacg atggtggagg aaaggcgggt
      961 atcgttgatc aaataagggg tcagtttgat ttgttcgccc atctcgccgt ccaaatcggt
     1021 acggcggaag gaaatcccgc tgttttcagg cgcggggtgc agggtcagtg cgacccgttc
     1081 gccggaatgc agcccgactc cggtaacgct gatggatttc gccaaagttc tttgcagcat
     1141 aaaccgctcc cttatcaatg gggtaagttt tggaataata cgataaaacc ggaaaaacag
     1201 gctatgtttt tccatagtat ttgccaatgt atccgttttc aatacgtaag ccgcataaaa
     1261 atgtatagtg gattaacaaa aatcaagaca aggcgacgaa gccgcccctc ccgaaaaacg
     1321 caaaaaatgc cgtccgaaaa cctttcggac agcattttcg cgtgaaccgt cattcccaca
     1381 aggacaaaaa accaaaacag aaaaccaaaa acagaaacct aaaattcatc attcccacga
     1441 aagtgggaat ctagttcgtt gagtttcagt tatttagaat aaattttgaa acttaaatcc
     1501 cgtcattccc acgaacctac attccgtcat tcccacgaaa gtgggaatcc agaatctcag
     1561 actttcagat aacctttgaa tattgctgtt gttctaag
//
LOCUS       DAWHRA010000218         1563 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWHRA010000218 DAWHRA010000000
VERSION     DAWHRA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.218"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..252)
                     /locus_tag="WIB30_002055"
     CDS             complement(<1..252)
                     /locus_tag="WIB30_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3798054.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITR"
     gene            complement(314..985)
                     /locus_tag="WIB30_002056"
     CDS             complement(314..985)
                     /locus_tag="WIB30_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3798055.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          375 a          344 c          394 g          450 t
ORIGIN      
        1 gcgggtaatg aggctgtgtt cgagttcggg atcggagagg ctgtgccatt gtccgagcag
       61 gacggctttg aacatggaca gcaggggata ggcgggacgg ccgcggtggt ctctaaggta
      121 acgggttttt tgacggttca ggtactgctc gatcggctgc caatcaatca cctggtccaa
      181 cttcaatagc gggaaacggt cgatgtgtct ggcaatcatg gcttgggcgg tttgctggaa
      241 gaaggtgctc atggaaaatc ccctaaatgt cttggtggga atttaagggg ttttggggaa
      301 ttttgcaaag gtctcagtct atgcccgata tacaattttg atacacaaac ttggaaatat
      361 cggtatcgtc gccggagcga tagaatgcgg acagtttttc attgaattgc ggcatctgca
      421 tttcggagat ttccaagatg ccgcagcccc ccgccatcat cagaccgttg gcaaataagg
      481 ttgccgttcg tttgttgccg tcccaaaaaa cctgctgccg catacaataa agcataaaac
      541 ggacggctgc ctccgtcgtc gaaccgcttt gcagtccgat attttgtaac acgcgggcca
      601 cttcaatttc cttcactgga ttcggggcat gacgggaacc gtccaatagc gttacgccga
      661 ccgaaccggt acggaaatca ccgggtgcca aagaatcgtc cttggcaaca atgttgttga
      721 tttttttaag gagtgaaata tcgaccggtt cgccgtttga aatatgcgaa atcacatatt
      781 gataggcacg tttcaggttc aagatggttt ggatgtcttc cagtgacgcg gaggctacat
      841 ttttgccatg gataatttgt tcggtctgca acaaggtcgt ctgacaattc tcaaaacggc
      901 tgaggttgtg aatttgggca accaatactt tctttgccaa aaaaatattt tctttcaaac
      961 tcaacttgta tttgtcggga aacacgataa ttccttaaat aattaaagca gtacgttaaa
     1021 tcatcatctt atcatcactt tcggggttta tgttgcgtgc gtccgttttc taatacggtt
     1081 tcccatcccg cgaaacgagg cggaggcctg tgcaaaaaac ctgttcacga aattttccgc
     1141 attaacgata cgctgaaaat gcgctaaaaa tgactgtgtt tgaatatcgg ttgatttcat
     1201 ccgtttgtgt aacctcgcgc cggttttgtc atgattttgc attatagtga attaaattta
     1261 aaccagtaca gcgttgcctc gccttaccgt actatctgta ctgtctgcgg cttcgtcgcc
     1321 ttgtcctgat ttttgttaat tcactatatt tttgtcatgc ggatatttca cgggatgaca
     1381 aaacgggcgc aaaaaagccc gattggaaaa tccgaatcgg gcttttttgt gatgttctgt
     1441 tcgatttgcg tactaactgc cggccggagc gtccgcgccc ggtttggcag gtcggattct
     1501 cgaatccgac ggctatttga gatggcaggc ttttgtcgga tacaagtatc cgacctacgg
     1561 ctt
//
LOCUS       DAWHRA010000219         1561 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWHRA010000219 DAWHRA010000000
VERSION     DAWHRA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.219"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(43..>1561)
                     /locus_tag="WIB30_002057"
     CDS             complement(43..>1561)
                     /locus_tag="WIB30_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ3798056.1"
                     /translation="EQEINEILHDKEKLLSLDNPRRFIFSKWTLREGWDNPNVFQICK
                     LRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFVKQLVGEINDN
                     SFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAEDGYDKLKAAY
                     PEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQYKIKDEAEFV
                     DLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIRFNTMTYRELL
                     EKLAQTAKIRMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLLHHSFHKFELD
                     YRLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIFYDSDIEHENV
                     ANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFVIEAKGTDGAE
                     DLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAGGHSENGR"
BASE COUNT          341 a          370 c          369 g          481 t
ORIGIN      
        1 aaaatcccga agcagacggc acgcgccccg aacattcgtt ctttaacgcc cgttttcaga
       61 atgcccgcct gcgggcatat tttgcccgat cagttcggct atcctctcgc cgtcaaactg
      121 cgttttgaac accaccttga tttctttgga aatctcggca aacagctttt cggcatgttt
      181 gattttccgc tcttcgcttt ttcgcaaatc ttccgccccg tcagtccctt tggcttcaat
      241 cacaaagttc aggatctcgc cgcttttggt tttcacgata taggcaaaat cgggcgaata
      301 cgtgccgccg ccggcaacag ggattttgat ggagtttctc ggtattttgg taaatacgat
      361 tacgccttca atttggttgt tggcgacatt ttcatgttct atatccgaat cgtagaaaat
      421 ttcgtcgaag agatagccgg cggcaggccg gtgctccgta tcttcaaatc tgcccaaatc
      481 tgcttttttc accgcgcgcg gtttgccgtc tttatcggta aatttggtcg gatggatttt
      541 gctgccgaca agccggtaat ccagttcaaa tttatggaag gaatgatgaa gcaaaaaccg
      601 gttgaagccg tttttgattt gggcgatggt ctgcatattc aaaaaatcgc caatgttcag
      661 ttcgtcgcgg acgcgataaa acgcctgatg caaagtctgc atgcggattt ttgccgtttg
      721 tgccagtttt tccagaagct cacggtaagt cattgtgtta aaacggataa aatcttcatc
      781 ttcaaaactg tctatgcggc gggaaagcat aagcccgttg ttgatataag cttcgtttac
      841 cgccgtgcgt atgcctgcct gcgggaattt ggcggcgttt tcacgcaaat aggcggtaaa
      901 taaatcgaca aattcggctt catccttgat tttgtactgc aaaacggctt tatggtgaat
      961 cagctcccac aaggctttga gttcttcata tttgccttcg cgcatgatga tggtgtcttt
     1021 gccctcgtct ttggcgttgc tgactttgcc tttgtccaaa cctttgggga aggcttcggg
     1081 ataggcggct tttaatttgt catagccgtc ttcggcaaag ttttcattgt cgtcaatgat
     1141 gccgtctgaa aacagttggt ttaccaatac cagcggtttg atatcggggt atttttgcaa
     1201 tattttttgt ttcagctctt cggtaaactt tttggagatt tcttcctgaa aagaattgtc
     1261 gttgatttcg ccgacaagct gcttcacaaa gtctttttcg ctgctatcga caaaataatt
     1321 cagtttgtac ggtacatcgc gcacccgcgc catcagctcg tttaccggca ggcgcaggcc
     1381 gcgtccgact tcttgcagct tggaagtcgt gctgccgctg gaacgcagtt tgcaaatctg
     1441 gaaaacgttg ggattgtccc agccttcgcg cagcgtccat ttggaaaaaa taaagcggcg
     1501 cgggttgtcc aaagacagca gtttttcctt atcgtgcagg atttcattga tttcctgctc
     1561 g
//
LOCUS       DAWHRA010000220         1521 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWHRA010000220 DAWHRA010000000
VERSION     DAWHRA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.220"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(124..552)
                     /locus_tag="WIB30_002058"
     CDS             complement(124..552)
                     /locus_tag="WIB30_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ3798057.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(533..967)
                     /locus_tag="WIB30_002059"
     CDS             complement(533..967)
                     /locus_tag="WIB30_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ3798058.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKASGDDGVRKS"
     gene            complement(1120..>1521)
                     /locus_tag="WIB30_002060"
     CDS             complement(1120..>1521)
                     /locus_tag="WIB30_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ3798059.1"
                     /translation="DGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLAGIAP
                     HPRESGETKFKSRCFGGRSTVRKALYMATVAATRFEPLIRDFHQRPLSEGKPYKVAVT
                     ACMRKLLTISNARMRDYFAENDTAENGIRTA"
BASE COUNT          379 a          451 c          300 g          391 t
ORIGIN      
        1 atttgaaaac cccattaaac cgtctttaac ccatcgtcct gaacggtaga tgtttcaccc
       61 cctgcgaacc aaaaaaaaga ccgtctgaat acagacggcc aaagcctggt cacacatcac
      121 tgcctaaaat aaagctgcct gctgtgccgc aggtcggctc atttcattga taatcgtata
      181 tcccgttcgt gaagagatgc cgtatttagg gcatagcttc gtcatcgcca taagcccgct
      241 ctccttatca atatcgcgca atttgacaaa ctcctgataa aacctatggt ttctcaactg
      301 tattaacgcc ttaccgcacc gtgggacata caattcctcg ccaccatata cctgcaacag
      361 ctcatgtgtt ttcacttcgc cgatggcttc gaccaaaatt gccaaacgct cggtgtccac
      421 cttgccctta ccaaatttaa accgcgcccc gccaatcgcc ttgaccagct gttccgtcgc
      481 tgccagtccg atgacatcca caatgtccaa cacggtatcc ggcaataaat gttcaacttt
      541 ttcgaacccc atcatcccca ctcgcttttt ccgttttcct gttttccgca atctgcaacg
      601 cagcaaccag tttatgtagc tgcgtatcgt ctaaatattc gaccttatcc ttaccaaaca
      661 tccgccgcgc cattgcgtgt gcatagttcc aatgtttgcc gccgacggtc agcagggctt
      721 cgactttgtc caacattgcc gctgatgatg tccgacgcag atgtggttta ccgtgtgggt
      781 taccttttgc tttaggctta aatccgtgcg accgcatatc agcgacaaca gactcaagtt
      841 cggaaacatc catatccgca cacgaccgct tgcccgtcac acgctccaac accgcgcgat
      901 aggtaccgtc atccaagccc agctcctttt gagcaatctt aattttcgca atcaacgccc
      961 ggcgcattat ctctccaata caacatatag tataaattag tgtatattat accaataaaa
     1021 tacaatatat agtattaaga cgatgttttt gcgaaacgga caggcataaa aaagcaacta
     1081 tatttttcac cccgtcgggc aaaaatacca aaactcaaat caagccgtcc gaataccgtt
     1141 ttcggcggta tcgttttcgg caaaataatc acgcatccgg gcattcgata tcgtcagcag
     1201 tttgcgcata catgccgtaa cggcaacctt atacggctta ccctcggaca gcgggcgttg
     1261 gtggaaatcc cgaataagcg gttcaaaacg tgtcgctgcc acggtagcca tatacagtgc
     1321 cttacgcacc gtagaccttc cgccaaagca gcggcttttg aatttggttt ccccgctctc
     1381 tctcgggtgc ggggcaatgc cggccaaact cgctatccgt ttgtgcgaca gccgccccaa
     1441 ttcgggcagc atcgccatca gcgtagccgt cgttatcgaa ccgatgcctt tgatttgctc
     1501 tgccacttgg gctttgccgt c
//
LOCUS       DAWHRA010000221         1496 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWHRA010000221 DAWHRA010000000
VERSION     DAWHRA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.221"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1185
                     /gene="aldA"
                     /locus_tag="WIB30_002061"
     CDS             <1..1185
                     /gene="aldA"
                     /locus_tag="WIB30_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ3798060.1"
                     /translation="IVAEGGKTKDLARVEVMFTADYLDYQAEWARRYEGEIIQSDRPR
                     ENILLFKRPLGVIAGILPWNFPFFLIARKMGPALVTGNTIVVKPSSVTPINCHIFAEI
                     VDAVGLPAGVFNVVNGPGAEIGNALSAHPQVDMVSLTGSVEAGRQVMEAASANITKVS
                     LELGGKAPAIVLKDADLDLAVKSILASRVGNTGQICNCAERVYVHSSLKDAFIEKMTA
                     AMKGVRYGNPAEAEAGALEMGPLIEERAVKAVAEKVERAVKQGAKLVCGGKRAEGRGY
                     FFEPTLLTDTDNSMDIMKEETFGPVLPVSAFDTLDQVIALANDCEFGLTSSVYTTNLN
                     EAFYVTRRLQFGETYINRENFEAMQGFHAGWKKSGIGGADGKHGLEEYLQTQVVYLET
                     DI"
BASE COUNT          372 a          409 c          384 g          331 t
ORIGIN      
        1 atcgttgccg aaggcggcaa aaccaaagac ttggcgcgcg tagaagtgat gttcaccgcc
       61 gactatctcg attaccaagc cgaatgggcg cgccgttacg aaggcgaaat catccaaagc
      121 gaccgcccgc gtgaaaatat tttattgttc aaacgtccgc tgggcgtaat tgccggcatt
      181 ttgccgtgga acttcccttt cttcctgatt gcccgcaaaa tgggtcccgc cttagtaacg
      241 ggcaacacca tcgttgtcaa acccagcagc gtaaccccga tcaactgcca catcttcgcc
      301 gaaatcgtcg atgcggtcgg actgccagca ggcgtgttca acgttgtgaa cggtccgggt
      361 gcggaaatcg gcaatgcctt gtccgcccat ccgcaagtcg atatggtcag cctgaccggc
      421 tccgtcgaag cgggccgcca agtgatggaa gccgcttccg ccaacatcac caaagtttcg
      481 ttggaactcg gcggcaaagc ccccgccatc gttttgaaag acgcggattt ggatttggcg
      541 gtgaaatcca tcttggcttc gcgtgtcggc aacaccggtc aaatctgcaa ctgcgccgag
      601 cgcgtttatg tccacagcag tctgaaagac gcattcattg aaaaaatgac tgccgccatg
      661 aaaggcgtgc gctacggcaa ccctgccgaa gccgaagcag gcgcgctgga aatgggcccg
      721 ctgattgaag agcgcgccgt caaagccgtt gccgaaaaag tggaacgggc agtcaaacaa
      781 ggtgcgaaac tggtctgcgg cggcaaacgc gccgaaggac gcggttattt cttcgagccg
      841 accctgctga ccgacaccga caacagtatg gacattatga aagaagaaac cttcggcccc
      901 gtgctgcccg tttccgcttt cgacacgctc gaccaagtca tcgcactggc taacgattgc
      961 gagtttggtc tgaccagttc tgtttatacg actaatttaa acgaagcctt ctacgttacc
     1021 cgccgcctgc aattcggcga aacctacatc aaccgcgaaa acttcgaagc catgcagggt
     1081 ttccacgccg gttggaaaaa atccggtatc ggcggcgcgg acggcaaaca cggtttggaa
     1141 gaatatctgc aaacccaagt cgtttatttg gaaaccgaca tttaatgccg ctttaaaacc
     1201 ccgatagaaa atgccgtctg aacccgtttt caggttcaga cggcattttt attgcttcat
     1261 cggcaatcag tcatgaccga ggtcgatgtt tttgtctttg tatagcggat taaatttaaa
     1321 cgaagccgca gacagtacag atagtacgga accgattcac tcggtgcttg agcaccttag
     1381 agaatcgttt ctttgagcta agtcgaggca acgccgtacc ggtttttgtt aatccgctat
     1441 attccgccat ctctaagatt tacagcgata cacgggtgat ttaaggaatg cccgaa
//
LOCUS       DAWHRA010000222         1480 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWHRA010000222 DAWHRA010000000
VERSION     DAWHRA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.222"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..79)
                     /locus_tag="WIB30_002062"
     CDS             complement(<1..79)
                     /locus_tag="WIB30_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3798061.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTA"
     gene            686..>1480
                     /locus_tag="WIB30_002063"
     CDS             686..>1480
                     /locus_tag="WIB30_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ3798062.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNYHRYGENDY
                     STSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNN"
BASE COUNT          493 a          254 c          288 g          445 t
ORIGIN      
        1 gggcggtaac ttcgagtaca aaaaaacgga gcagttcttt ctgtactttt ttctttaatt
       61 tgcagtgtgt tatcttcata tttcgagggt aacatatctg ctaatctagt acagccccaa
      121 aaatatacca aaaacagcaa aacaaattgt aaggataggt ataggctttg taaaggtaaa
      181 ttgtgaaaaa agcagttttt taaacgaatg aaacggcttc gggctgaaat atatgctgat
      241 gccctgtcct tcccgtatat cttgtgtgtt gtcaaagtgc aggctgcttt gaaattggca
      301 ttacagtcga tgaaccacca ttttgcttta tttcagcggg cttgagatgt gtataagaat
      361 attgttttga ataaatttaa agaaaatgat aatcgttatt gacgattttt aaaggaaagc
      421 gtagagtgcc aattctatga agcaatacgg taagtaacaa tgaaaatatc tactgcttgg
      481 gtatagagca tatttcacaa cccgtaacta ttcttgcgga aacagagaaa aaagtttctc
      541 ttctatcttg gataaatata tttaccctca gtttagttaa gtattggaat ttatacctaa
      601 gtagtaaaag ttagtaaatt atttttaact aaagagttag tatctaccat aatatattct
      661 ttaactaatt tctaagcttg aaattatgag accatatgct actaccattt atcaactttt
      721 tattttgttt attgggagtg tttttactat gacctcatgt gaacctgtga atgaaaagac
      781 agatcaaaaa gcagtaagtg cgcaacaggc taaagaacaa accagtttca acaatcccga
      841 gccaatgaca ggatttgaac atacggttac atttgatttt cagggcacca aaatggttat
      901 cccttatggc tatcttgcac ggtatacgca agacaatgcc acaaaatggc tttccgacac
      961 gcccgggcag gatgcttact ccattaattt gatagagatt agcgtctatt acaaaaaaac
     1021 cgaccaaggc tgggttcttg agccatacaa ccagcaaaac aaagcacact ttatccaatt
     1081 tctacgcgac ggtttggata gcgtggacga tattgttatc cgaaaagatg cgtgtagttt
     1141 aagcacgact atgggagaaa gattgcttac ttacggggtt aaaaaaatgc catctgccta
     1201 tcctgaatac gaggcttatg aagataaaag acatattcct gaaaatccat attttcatga
     1261 attttactat attaaaaaag gagaaaatcc agcgattatt actcatcgga actatcatag
     1321 gtatggagag aacgattaca gcactagcgt aggttcctgt attaacggtt tcacggtacg
     1381 gtattacccg tttattcggg aaaagcagca gctcacacag caggagttgg taggttatca
     1441 ccaacaagta gagcaattgg tacagagttt tgtaaacaat
//
LOCUS       DAWHRA010000223         1474 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWHRA010000223 DAWHRA010000000
VERSION     DAWHRA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.223"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(109..1104)
                     /gene="porB"
                     /locus_tag="WIB30_002064"
     CDS             complement(109..1104)
                     /gene="porB"
                     /locus_tag="WIB30_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ3798063.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVEHNGGQV
                     VSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGKYNSESYHAGFNYKNGGFFVQYGGAYKRHVRVDENVNIEKYQIHRLVS
                     GYDNDALHASVAVQQQDAKLVKDNYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSF
                     DDADLSNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVSTAGGVGLRHKF"
BASE COUNT          370 a          337 c          370 g          397 t
ORIGIN      
        1 aaaagccaaa cagaaaaaag ccaaacagaa aaaagccaaa cagaaaaaag ccaaacagaa
       61 aaaagcctgt ctggcgacag gctttttgtt gataccaatc tttgcagatt agaatttgtg
      121 gcgcagaccg acaccgccgg cagtcgatac gaatttgttt tcgcctttac cttcttgcaa
      181 ccaaccggca gaaaccaagg cagaagtgcg tttggagaag tcgtattccg caccgacaac
      241 cacttggtcg taatcgttgc ttaagtctgc atcatcaaac gagcctttga agccgtgggc
      301 gtaagaaacg cggggcgtta cgttgccgaa gcggtatgcc aaggtagcgg caacttcggt
      361 ttgagagttg tgcgaataat tgtctttaac caatttggca tcttgttgct gtacggctac
      421 ggaagcgtgc agggcatcat tgtcgtaacc gctgaccaaa cggtgaatct ggtatttctc
      481 aatattcacg ttctcatcca cccgcacatg tcttttatag gcaccgccat attgcacgaa
      541 gaagccgccg tttttgtagt tgaagccggc gtggtaagat tcgctgttat atttgcctgc
      601 attgtcgtta agcgcgtatt gtacgctgcc gctgaggccg gcaaattcgg gagaatcgta
      661 gcgtacggaa atgaggcgtg cttcgggttc ggcaattttg tttacaccca aatagtcgct
      721 tttgctatcc caaggattga tgtcgccggt gtctttcagg acgctgttca aacggccgac
      781 gcgcaattta ccgaagccgc ctttcaaacc gatgaaggat tggcggttgc cccaaccgga
      841 gtcagtaccg gcgatagatg ccttttgctc aacctgccaa atggctttca gaccgttacc
      901 gaggtcttct tggcctttga agccgatttt tgaacccaaa tcaacgatgc cggtaccggt
      961 ttcaacgcta accacctgac ctccattgtg ctctacagag cgggaagttt ctacgccggc
     1021 tttgatggtg ccgtacaggg taacgtcagc cattgctgca acaggaaggg ctgccaaagt
     1081 cagggcaatc agggattttt tcattgctgt attccttttt tggttaagaa atttaagcag
     1141 gccgggcttt ccaagccgct tagctttgca tttaccgccg acgttttcgg cgattccatg
     1201 tctgaaacta tagatgttcc tgtctgaaaa aacaaacttc tgcctgtttt tgtgtggaat
     1261 tcgatgtgta ttttgaaggg cggatagaga tattgtgggt attttttgtt ttataacata
     1321 tggttattta aattttttaa gatttgcatt tttacaacac ttactcggga gggtattgga
     1381 gggcattgca aaccgggggt tataaagacg gcaaaaaacg cacacgcggt ttctttcgga
     1441 acgggcggat gcaaaacccg accctgcggc agga
//
LOCUS       DAWHRA010000224         1467 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWHRA010000224 DAWHRA010000000
VERSION     DAWHRA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.224"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>1467
                     /locus_tag="WIB30_002065"
     CDS             <1..>1467
                     /locus_tag="WIB30_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ3798064.1"
                     /translation="PALWFGPVQNGKVQMYSASVSTYPDSSSSQIFLQNLSRKDDTSK
                     PGRYSLKPLSTSEIKSKEPTFTGRQTVIRLDGGVQQIKLQGNEVTSFNVNNGNNTFGI
                     VKDLGVDPDASEWKKVLLPWTVRASNDDNQFKTINQQLNQQKIQYSQRYRIRDNGNRD
                     LGDIVNSPIVAVGEYLATSANDGMVHIFKQSGGDKRSYNLKLSYIPGTMPRKDIQNTE
                     STLAKELRTFAEKGYVGDRYGVDGGFVLREVELSGKKHVFMFGAMGFGGRGAYALDLT
                     KADSNNPTAVSLFDVKNDKNSNNGVQLGYTVGTPQIGKTHDGKYAAFLASGYATKEIT
                     SNDNKTALYVYDLESNGTLIKKIEVPGGKGGLSSPTLVDKDLDGTVDIAYAGDRGGKM
                     YRFDLSGNNPNSWTVRTIFEGTKPITSAPAISQLKDKRVVIFGTGSDLSEDDVDNNDM
                     QSIYGIFDNDTDTGVAKDGQGNGLLEQVLSEENKTLFLT"
BASE COUNT          411 a          377 c          373 g          306 t
ORIGIN      
        1 ccccgcgctg tggttcggac ctgtgcaaaa tggcaaggtg cagatgtatt ccgcttcggt
       61 ttccacctac cccgacagtt cgagcagcca aattttcctg caaaaccttt cccgcaagga
      121 tgacacaagc aaaccgggcc gctattccct caaacccttg agtacgtcgg agattaaaag
      181 taaagagccg actttcacag ggcggcaaac cgtcatccga ttggatggcg gcgtacagca
      241 gattaaactt caaggcaatg aggtcactag ttttaatgta aacaacggca acaacacttt
      301 cggcattgtt aaggacttgg gcgtcgatcc tgatgccagc gagtggaaaa aagtgctgct
      361 gccttggacg gttcgtgctt ccaatgatga caatcaattt aaaacaatta accaacaact
      421 taaccaacaa aaaatccaat acagccaaag ataccgcatc cgcgacaacg gcaatcgcga
      481 tttgggcgac atcgtcaaca gccccatcgt ggcggtcggc gagtatttgg ctacttccgc
      541 caacgacggg atggtgcata tcttcaaaca aagcggcggg gacaagcgca gctacaatct
      601 gaagctcagc tacatccccg gcacgatgcc gcgcaaggat attcaaaaca ccgaatccac
      661 ccttgccaaa gagctgcgca cctttgccga aaaaggctat gtgggcgacc gctacggcgt
      721 ggacggcggc tttgtcttgc gcgaagtcga actgagcggg aaaaaacacg tgtttatgtt
      781 cggcgcgatg ggctttggcg gcagaggcgc gtatgccttg gatttgacca aagccgacag
      841 caataacccg accgccgttt ccctgtttga tgtcaaaaat gacaagaata gcaataacgg
      901 cgtgcaatta ggctacaccg tcggcacgcc gcaaatcggc aaaacccacg acggcaaata
      961 cgccgccttc ctcgcctccg gttatgcgac taaagagatt accagcaacg ataataaaac
     1021 cgcgctgtat gtgtatgatt tggaaagcaa cggtacgctg attaaaaaaa tcgaagtacc
     1081 cggcggcaag ggcgggcttt cgtcccccac gctggtggat aaagatttgg acggcacggt
     1141 cgatatcgcc tatgccggcg atcgcggcgg caagatgtac cgctttgatt taagcggcaa
     1201 caacccgaac agttggactg tacgcactat ttttgaaggc acaaaaccga ttacttccgc
     1261 gcctgctatt tcccaactga aagacaaacg cgtggtcatc ttcggcacgg gcagtgattt
     1321 gagtgaagat gatgttgata ataatgatat gcaatctatt tacggtattt ttgacaatga
     1381 cacagacaca ggtgtggcga aagacgggca gggcaacggg ctgctcgagc aagtgcttag
     1441 tgaggaaaat aaaaccttat tcctgac
//
LOCUS       DAWHRA010000225         1431 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWHRA010000225 DAWHRA010000000
VERSION     DAWHRA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.225"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(73..759)
                     /locus_tag="WIB30_002066"
     CDS             complement(73..759)
                     /locus_tag="WIB30_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ3798065.1"
                     /translation="MYAFTDAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHDGGWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIALVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(836..1309)
                     /gene="queF"
                     /locus_tag="WIB30_002067"
     CDS             complement(836..1309)
                     /gene="queF"
                     /locus_tag="WIB30_002067"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ3798066.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     regulatory      complement(1314..1358)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT          352 a          302 c          442 g          335 t
ORIGIN      
        1 cggatttatc cgttccggca gtgtttgcaa ataaaagaaa acccaaccgt cccgattccc
       61 ggcagggctg tgttacggat tttgcagcga gggcgcgggg cggtcttgcg cctgtttggt
      121 ttgcagggtc gtcagttttt tcgtcagcag attcagaatc acgccgtagg cgggcaggaa
      181 aaacagaccg cagacggtga gtttgaacag gtaatcgacc aaggcgatgc cctgccagtt
      241 tgccgccata aattcatcgc tgcttgcgta gaaggcaacg gcgaaaaata ccagcgtatc
      301 caaggcgttg ccgatgacgg ttgatgcggt cggggcaatc caccacgctt tcagacggcg
      361 taatttgttg aatacaaaaa tatcaaggat ttgtccgagc gcataggcgg caaaacttgc
      421 cagcgcgatg cgtccgacaa aggtgttgaa ttcggacagt gcgcccaagc ccgtccaacc
      481 gccgtcgtgg aacaaaacgg aaaagacgta ggaaagcaaa agggcgggga acatcaccca
      541 aaagataatc cgccgtgcca agtgcgaacc gaaaatgcgg acggtcaggt cggtggcaag
      601 gaagatgaag ggaaaggaaa atgcgcccca agtggtgtgg atgccgaaaa tttggaaggg
      661 gaactgcacc agatagttgc tggcggcgat gataaggata tgaaaaagca ccagccggaa
      721 gagtgccttc tgttgctgtg cgtcggtaaa tgcgtacata aaaaaacctt tcggaaaggc
      781 gttcagacgg cataccgtat cgaaagaatg ccgtctgaaa tatgggaagg atggtttatt
      841 gtgcgtcgtg ctcaaacagg cgtttacgcg ccaatgcttc aaactcggtg cctgctttgc
      901 cgtaattggc gaaaggatga atggcgatgc cgccgcgcgg tgtgaactcg ccgaatactt
      961 cgatgtattt cggatccatc agggcaatga ggtctttcat gatgatgttg acgcagtctt
     1021 catgaaaatc gccgtggttg cggaagctga agaggtagag tttcagggat ttgctttcca
     1081 ccattttgat gtgcggaatg tagcggatga cgatggtggc gaagtcgggc tgcccggtca
     1141 tggggcagag gctggtgaac tctgggcaga cgaatttgac gaaatagtcg ttgtcgggat
     1201 gtttgttgtc gaatgcttcg agaatttcag gcgcgtagcc ggtcggatat tgggtttttt
     1261 gattgcccaa aagagagatg ccttgcagct cttcgttgtt gcgggacatg agggtttcct
     1321 tagtttttta atgtgggagg ttttcgaacc acgggcggcg attgtaatat aagcggcggt
     1381 atctgtgtag ttttcttcag acggcatggt ttggacggcg gcgttttccg t
//
LOCUS       DAWHRA010000226         1429 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWHRA010000226 DAWHRA010000000
VERSION     DAWHRA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.226"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            223..453
                     /locus_tag="WIB30_002068"
     CDS             223..453
                     /locus_tag="WIB30_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798067.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDAAAIGTQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            481..780
                     /locus_tag="WIB30_002069"
     CDS             481..780
                     /locus_tag="WIB30_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798068.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPKCSNYEQVKSGAYLGSMVLILFVVIYGFRLLINFLKDIGKVGA"
     gene            899..1219
                     /locus_tag="WIB30_002070"
     CDS             899..1219
                     /locus_tag="WIB30_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ3798069.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
     gene            1203..>1429
                     /locus_tag="WIB30_002071"
     CDS             1203..>1429
                     /locus_tag="WIB30_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ3798070.1"
                     /translation="MCCSLSFAEPARIDDRIIKFRPSKSKFFESPGYRKINNEFSKFT
                     EAANVEHIPTGAKARINAKITASVSRAGVLAG"
BASE COUNT          444 a          257 c          302 g          426 t
ORIGIN      
        1 aaaacaggct cggcgggcgg tctgtcaacc tttcacaaag cccgcaaaaa ggattaaaaa
       61 tgaataaagc aactttaatt atcggatttt ttgtttgcct cattcttttc attttctcaa
      121 ttctctattt cataaattaa aaacaaaatt cgggagcagg cttcgcccgg atttaaacct
      181 aagaagccgt cttactttaa atatcaaaaa aggaaaaaaa cgatgaacat cgttaaaaaa
      241 tacgctgtaa aagcagcctt ggcagccggt atcttcacac cggccattgt tatggcagat
      301 acctttgatg cagccgcaat tggtacgcaa gtagcgaatg taatcatggg tttcgtgtca
      361 atggtttccg ccgtgggtat ggcggccatt accgtgattc ttgcaatcca aggcttcaaa
      421 atggcttgga gcatgattaa atctgtcaaa taaacagagt gaagaaaaag gggcgcataa
      481 atgggctatc gtgtcggcat aaattgtttt gatacaagat tgcaggcaga cgactattta
      541 ttgtcgtccc ttcctcctac tgttacccag gacggaaaaa tcatcaggcc ggaaagggtg
      601 ggcgataaat ggattttgaa cggaaagccg gtcacgctgt cttatccgaa atgttcgaat
      661 tacgaacaag ttaaatccgg agcttatctc gggtctatgg ttttaattct gtttgtcgtt
      721 atttacggct tcaggctgct gattaatttc ttaaaagaca taggcaaagt aggggcgtga
      781 tgatgtttgt cgatttttgg tttttgctcg gatttttcct agctttgtct gtcgctttga
      841 tatttatatg acgtgtttta aaatcaggct ttcaaaacaa cctttgaaag acagaaacat
      901 gaacaagccg tttatcacgc aggcgcagtt ggcactttat aaatatcagc cgtccagcaa
      961 gtattttggg cagtcgatgg cattaattgc gtctaaggaa tttgaagagt ttgtaagaaa
     1021 tgtaaaagaa tacgacgtaa tagaatgttt ctcttatttt ttaaataaga gggtaacgca
     1081 taatatttgg aaaatttatt tttctgatga gtctaatatt tttattagga agtcagaaga
     1141 aaatggaaaa atttcgcatg aatttattta ctcggaattt tctgatagca acaccgattt
     1201 taatgtgttg ttctcttagt tttgcagaac cagcaagaat agatgatcga ataataaaat
     1261 ttaggccatc taaatcaaag ttttttgaat ctccaggata tagaaaaatc aataatgaat
     1321 tttctaaatt cacagaagcg gcaaatgtcg aacatatccc cacgggcgca aaagcccgaa
     1381 tcaacgcaaa gataaccgcc agcgtatccc gcgccggagt cttggcagg
//
LOCUS       DAWHRA010000227         1403 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWHRA010000227 DAWHRA010000000
VERSION     DAWHRA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.227"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..296
                     /locus_tag="WIB30_002072"
     CDS             <1..296
                     /locus_tag="WIB30_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ3798071.1"
                     /translation="QGIQKTARNDFLQSMGFKTRFDSLEYRQIKAVAYELLEKHLPEE
                     DFERARRFLYQCFKRTDTPPAGVWLDFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            338..1180
                     /locus_tag="WIB30_002073"
     CDS             338..1180
                     /locus_tag="WIB30_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ3798072.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLKERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT          369 a          316 c          373 g          345 t
ORIGIN      
        1 cgcaaggcat ccaaaaaacc gccagaaacg attttttgca gtctatgggt tttaaaaccc
       61 ggttcgacag ccttgaatac cgccaaataa aagcagtagc gtatgaattg ctggagaaac
      121 atttgccgga agaagatttt gaacgcgccc gccggttttt gtaccaatgc ttcaaacgga
      181 cggacacgcc gcccgccggc gtgtggctgg attttgcggc agacggcagg atgcggcggc
      241 tgtttacctt gaggcaatac ttcggcattt tgcaccgatt gctgaaaaac cgttgaaaaa
      301 cgccgcttta tccaacagac aaaaaacagg ataaattatg caaaaccacg ttatcagctt
      361 agcttccgcc gcagaacgca gggcgcacat tgccgcaacc ttcggcgcgc gcggcatccc
      421 gtttcagttt ttcgacgcac tgatgccgtc tgaaaggctg gaacaggcaa tggcggaact
      481 cgtccccggc ttgtcggcgc acccctattt gagcggagtg gaaaaagcct gctttatgag
      541 ccacgccgta ttgtggaaac aggcattgga cgaaggtctg ccgtatatcg ccgtatttga
      601 ggacgacgtt ttactcggcg aaggcgcgga gaaattcctt gccgaagacg cttggctaaa
      661 agaacgcttt gaccccgatt ccgcctttat cgtccgcttg gaaacgatgt ttatgcacgt
      721 cctgacctcg ccctccggtg tggcggacta ctgcgggcgt gcctttccgc tgttggaaag
      781 cgaacactgg gggacggctg gctatatcat ttcgcgtgag gcgatgcggt ttttcttgga
      841 aaggtttgcc gttttgccgg cagagtggat taaagcggta gattggatga tgtttactta
      901 tttctttgat aaggagggga tgcctgttta tcaggttaat cccgccttat gtacccaaga
      961 attgcattat gccaagttcc tcagtaaaaa cagtatgttg ggtagcgatt tggaaaaaga
     1021 tagggaacaa gaaagaagac accgccgttc gttgaaggta atgtttgact tgaagcgtgc
     1081 tttgggtaaa ttcggtagag aaaagaagaa aagaatggag cgtcaaaggc aggcggagct
     1141 tgagaaagct tacggcaggc gggtcatatc gttcaaatag tttgtgtaaa atatagcgga
     1201 ttaaaattag aaatggacac actgtcattc ccgcgcaggc gggaatccag tccgttgagt
     1261 ttcagttatt tccgataaat caccgcaacg ttaaatttct agattcccgc ttttgcggga
     1321 atgacggcgg gggatttctg tttttccgac aaattcctaa aacttaaaat tccgttattc
     1381 ccgcgaaaac agaaaaccaa aaa
//
LOCUS       DAWHRA010000228         1388 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWHRA010000228 DAWHRA010000000
VERSION     DAWHRA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.228"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            92..1222
                     /locus_tag="WIB30_002074"
     CDS             92..1222
                     /locus_tag="WIB30_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ3798073.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGRGDLERLAARDIIRFPGFVNNVSEVIKEH
                     HIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAE
                     KMIYFIENREAIRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          377 a          328 c          322 g          361 t
ORIGIN      
        1 tacgcaggca aacccaagcc ttgctgggtt acaggatgaa accctgccgt agcagaccgc
       61 atttccaatc catattcaag gtgtccgcct tatgaaaatc gtttttatca caacagtcgc
      121 atccagcatt tacggtttcc gcgcccccgt cattaaaaaa ttaatcggca aaaaccatca
      181 ggtgtatgcc tttgtatcgg agttttccga taatgaattg gatattatca gggaaatggg
      241 ggttacaccc gttacctacc gttcaaaccg cagcgggctg aacccgtttt cggatataaa
      301 atccaccttc ctcatcttta aagaactcaa aaaaatatcg ccggatttgg ttttccctta
      361 tttcgcaaaa cccgtgattt tcggcacttt tgccgcaaaa ctggcaggcg tgcccagaat
      421 cgtcgggatg ctggaaggtt tgggattcgc atttaccccg cagccggaag gcataccgtt
      481 aaaaacaaaa atcataaagg ggattttgat tgccttatac cgcattgccc tgccgatgtt
      541 ggaaagcctg attgtattaa accccgacga caaagacgaa ctgacggaca aatacggcat
      601 caaaataaaa aacatccata ttttgggcgg aatcggtctg gatttgcggc aatatcctta
      661 ttccgaggcg gatattcccg atgaaaaaga acccgtaaaa tttctcttta tcggcagatt
      721 tctgaaagaa aaagggattg atgattttat tcgggcggcg gaacaggtta aggacaaata
      781 ccccgatacg gtttttaccg ctttgggcgc aatcgacaaa tcacgcgggg ggaggggcga
      841 tttggaacgg cttgccgccc gcgatattat ccgtttcccc ggttttgtga acaatgtttc
      901 cgaagtgata aaagaacatc atatattcgt attgccgtct tattataggg aaggcgttcc
      961 ccgaagcact caggaggcaa tggccgtcgg cagggcggtg attacgacgg atgtccccgg
     1021 atgcagggaa acggtcgccg acaaggtcaa cggcttcctg atcgaacctt ggaatccccg
     1081 catcttggcc gaaaaaatga tttattttat cgaaaacagg gaagccatcc gcctgatggg
     1141 gaatgcaagt tatgcgattg ccaaagataa attcgatgcc gaaaaagtcg atttgaaatt
     1201 actcgatatt ttgaaggcgt aaacaaggct gcccgctttt gagtttcggt catttctgat
     1261 aaacccccgt cattcccgcc acacccccgt cattccctcg aaagcgggaa ttcaggttcg
     1321 tttagtttcg gtcatttccg ataaattctt gcaactttgc gtttctagat tcccactttc
     1381 gtgggaat
//
LOCUS       DAWHRA010000229         1374 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWHRA010000229 DAWHRA010000000
VERSION     DAWHRA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.229"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(383..793)
                     /locus_tag="WIB30_002075"
     CDS             complement(383..793)
                     /locus_tag="WIB30_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798074.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIKGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(797..>1374)
                     /locus_tag="WIB30_002076"
     CDS             complement(797..>1374)
                     /locus_tag="WIB30_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ3798075.1"
                     /translation="VAEAAGTVWRGKKVELNPTKWDWVKNTGYKKPAARPMQTLDGEM
                     AGGNKPAKQATPNGKAGVSTQQSLQNSLVPQKNYTHTTYNGGSAITDGKYITDPLSMV
                     RHKPDASSLDKIDSKSLFKSDVDAEKATLDAARYADKHNLWDSQNKAKVPTNDIVGYT
                     DGKPTHIINIYRRKPNKDGFRPVHGTPGKER"
BASE COUNT          394 a          262 c          232 g          486 t
ORIGIN      
        1 ctctacggat ggggcggagt tacccgtatg attgccgtat ggtcggatac ttgtatccga
       61 caaaaatatt tgacgcgtct atcgtttccg aaatgccgct gttgaaaaac gtcggattcg
      121 agaatccgac ctacggttgc tgaacaatgt tttgtagaac ttgctcaaaa tccatgtttc
      181 gccattttcc tttatatata cctaatgcag cacgggttac gatttcgttt aagtcataaa
      241 tagccattta cgtcctctgc taattttcag cgagtagatt gggttaccaa acccaacaaa
      301 aaagcagcct gaaaaatttc aggatgcttg ttttatgttt tttatcagtg aattaggttt
      361 taatccagcc tgcgcttgct agttaaacag tatttacata aaaatcttgc actgaacaaa
      421 caagcgtatc atgttcatca tgaaatgatg ggcataacgt taatctacaa atgtttacat
      481 ttaaagatat tttttcagtt tcaatatttt taatttgtaa ggttaataca tgaggatatt
      541 tgtgatatag ctgatttttt aattgttcgt tgtctaatat agcaaaaggt aattttgcac
      601 acaaataacc aataactttg tttttatgaa aaaaaactat ccaattttca attccttcat
      661 cccaatcaaa agtaaaatcc gttcctttta tgataaattt agcacaagat attaaatcta
      721 acttaaccca agtggattta tacagactca atgctgatga agttataata ttatctaaat
      781 tataaatttt cataaatcaa cgttccttac ctggtgttcc atgcacaggt ctaaacccat
      841 ctttattcgg ctttctacga taaatgttaa ttatatgtgt cggttttccg tctgtatagc
      901 caactatgtc gttcgttgga acttttgcct tattttgact atcccataaa ttatgtttat
      961 ctgcatatct agcagcatct aatgtagctt tttccgcatc aacatctgat ttaaataaac
     1021 ttttgctatc tatcttgtct agactagatg catcaggttt gtgtcgtacc atactgagag
     1081 ggtcagtaat atatttacca tctgtaatag ctgatccacc attataggta gtatgcgtat
     1141 aatttttttg tggaacgaga ctattttgca aagattgttg tgtagaaacg ccagctttgc
     1201 cattaggagt agcttgcttc gctggcttat tcccaccggc catttcccca tctaaagtct
     1261 gcatagggcg ggcagcaggt tttttataac cggtattttt aacccaatcc catttagtcg
     1321 ggttaagttc tactttttta cctctccaaa ccgtacctgc ggcttctgct acgg
//
LOCUS       DAWHRA010000230         1369 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWHRA010000230 DAWHRA010000000
VERSION     DAWHRA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.230"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(251..>1369)
                     /locus_tag="WIB30_002077"
     CDS             complement(251..>1369)
                     /locus_tag="WIB30_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ3798076.1"
                     /translation="GAQVAVMAPTEILAEQHFIKFKQWLEPLGIEVVRLFGSLRKKAK
                     DEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQHRFGVAQRLALKNKGREVH
                     QLMMSATPIPRTLAMSFFADLDVSVIDELPPGCTPIKTRLVNNVRRAEVEGFVLGTCR
                     KGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIGLVHGRMKAAEKAEVMARF
                     SSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLHQLRGRVGRGAAESVCVLL
                     FAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLGARQSGVPMLRFAKLEEDL
                     HLLEQAREIAPILIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT          276 a          380 c          385 g          328 t
ORIGIN      
        1 gcttcaattt cgtcatttct ccggtttcag tcattgccga taacaccgtg gtttttcatt
       61 tctagattcc cgcctgcgcg ggaatgacgg cggagggctt gccgttttcc cgataagtct
      121 ctgcggcttc ccattgccgg atttccgccc acttgggaat aacgagattt aaaatcatga
      181 aaatgtgtca aaaataatgc aaaatcagat tccgttgcac ttttttccaa tatttccggc
      241 gacatttcgc tcacacgccc aaataacctt ccctgccgga aagccatcgt gccaaatgcg
      301 cttcgacgat ttcggggttt tgttcaatca gtatcggggc gatttcgcgc gcttgttcca
      361 aaaggtgtaa gtcttcttcg agcttggcga aacgcagcat aggcacgccg ctttgacgcg
      421 cgccgagaaa ttcgccgggg ccgcggatgt tgaggtcttg gcgggcgatt tcaaagccgt
      481 cggtgtgttc gtagatgact ttcagccgcg ctttggcgag ttcgcccaag ggttcggcaa
      541 acaggaggac gcacacgctt tctgccgcgc cgcgcccgac ccgaccgcgt aattggtgca
      601 gctgcgccaa gcccatgcgc tcggcgtgtt cgatgaccat cagggcggca ttgggcacat
      661 ctacgccgac ttcgatgacg gtggtggcga ccaagacgtt cagccccccc gaagaaaacc
      721 gcgccatcac ttcggccttt tcggcggcct tcatgcgccc gtgtaccagt ccgatattga
      781 gttcgggcaa tgccgtctga agccgggcga gggtttcggc ggcggtttgc agttgcaggg
      841 tttcgctttc ttcaatcaat gggcagaccc aatacgcctg ccgccctttt cggcaagtgc
      901 cgaggacgaa gccttcgact tcggcacggc ggacgttgtt gacgaggcgc gttttaatcg
      961 gtgtgcaccc gggcggcaat tcgtcgatga cggacacgtc caaatcggcg aaaaaactca
     1021 tcgcaagcgt gcgcgggatg ggcgtggcgg acatcatcag ctgatggact tcgcgccctt
     1081 tgtttttgag ggcgaggcgt tgggcaacgc cgaaacggtg ctgttcgtcc acaatggtca
     1141 agcccaaatt gtgaaacgcc acgccgtctg aaaacagggc gtgcgtgccg acggcgattt
     1201 tgacgctgcc gtcagccagt ttggctttgg cttcgtcttt ggctttttta cgcaaactgc
     1261 caaaaaggcg gacaacttca atgcccaaag gttcgagcca ttgtttaaat ttaataaaat
     1321 gctgttcggc aaggatttca gtgggcgcca ttacagccac ctgcgcacc
//
LOCUS       DAWHRA010000231         1279 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWHRA010000231 DAWHRA010000000
VERSION     DAWHRA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.231"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            289..894
                     /locus_tag="WIB30_002078"
     CDS             289..894
                     /locus_tag="WIB30_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ3798077.1"
                     /translation="MNTDFQNILKQLGKQAKKEAAEKQEAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPSDKTPIKVRPKDNRADEENYFYIGSTYNEPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            957..>1279
                     /gene="ssrA"
                     /locus_tag="WIB30_002079"
     tmRNA           957..>1279
                     /gene="ssrA"
                     /locus_tag="WIB30_002079"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          410 a          349 c          272 g          248 t
ORIGIN      
        1 tcagatttcc gtgtttcgcg tttccggcaa ttcaagattg gcaaaaccat taacctgctt
       61 cgcaaggcag gtttttaaaa ctttatcagg cgttcgggca tatcggttcg accggcccgc
      121 atttccggca atcaacactg ccgacggttg ccgcatttct ttttgatata caatcacact
      181 ttcaaaaaac atacgctggc aacaaaatca tcctcccccg cttggcaaac attccggcaa
      241 attaaaaccc cgcccgaaac agccatcgga caaataacag gaaaaaatat gaataccgac
      301 ttccaaaaca tcctcaaaca actgggcaaa caggccaaaa aagaagcggc agaaaaacag
      361 gaggccgaaa aaaacaagca aaaacaagaa caggatttcg atttttcaca agcagtcgga
      421 caagtctccc cgttaaaaaa ccggcaacaa tattatgcac cgtccgacaa aacccccatc
      481 aaagtccgtc ccaaagacaa ccgcgccgac gaagaaaact acttctacat cggcagcaca
      541 tacaacgagc cgcccgccag cttcagcaaa aacggacagg gaaaaaacga catccaacgc
      601 ctgaaaaacg gatactatcc cgtcgttacc gatgtcgacc tgcacggcta cacacaggaa
      661 gaagcccaaa aagtcctcaa cgaattcatc gcattcaccc aaaaacgcgg cgtatgcggc
      721 gaaatcatcc acggcagcgg attaggttcc aaaggctaca aacccgttct gaaaaatatg
      781 acccgaaact ggctgatgca acaccccgac gtactcgcct acgccgaacc ccgggaaggc
      841 aacgacggct gcgtccgcat cctgctcaaa cgcaaattaa ggcagcaaga ctgacaaatt
      901 tctccgacag acttggcagc accgcacaat atccgtataa tttgcaaacc atttctgggg
      961 gcgaccttgg tttcgacggg ggttgcgaag cagatgcggg cataccgggg tctcagattc
     1021 ccgtaaaaca ctgaattcaa atagtcgcaa acgacgaaac ttacgcttta gccgcttaag
     1081 gctagccgtt gcagcagtcg gtcaatgggc tgtgtggcga aagccaccgc aacgtcatct
     1141 tacattgact ggtttcctac cgggttactt ggcaggaaat aagacttaag gtaactggtt
     1201 tccaaaaggc ctgttggtcg gcatgatgga aataagattt tcaaatagac acaactaagt
     1261 atgtagaacg ctttgtaga
//
LOCUS       DAWHRA010000232         1274 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWHRA010000232 DAWHRA010000000
VERSION     DAWHRA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.232"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(90..641)
                     /locus_tag="WIB30_002080"
     CDS             complement(90..641)
                     /locus_tag="WIB30_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798078.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(695..1274)
                     /locus_tag="WIB30_002081"
                     /pseudo
     CDS             complement(695..1274)
                     /locus_tag="WIB30_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          369 a          394 c          238 g          273 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgccg gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggc agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccgaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaactttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaacaaggg gctgtcctag ataactagga caaacttgat tttactaatt
      721 gttttaaaat ggaacaagaa cttttatctc actgttgtta aaacgccatt cgcactcctt
      781 taaatacagc tcaaaatgcg ctttgggaat gccgttaaac ttgcgtaaat gacgttttgc
      841 ctggttccaa aagttctcaa ttccattaat atggttttgt cgttcggcaa aatgtgtgct
      901 gtgattgata cgaaaacgaa gtttcagcga agctaaaatg gctaaattcg cgcacatcta
      961 atacatcata gctacgataa caatccgtat aaacaatgct gtcaggtttc acttgttcac
     1021 ggataatagg aaataaagta gcggtttgag tattcggtac tgtaaccgta taaaccttac
     1081 catttcgctt caaaagaccg aatacggcga ctttaccggc agcaccgcga ccgcgtttgc
     1141 ctttgcgttg tccgccaaaa taactttcat ctgcttctac ttcgccatca aacatttcca
     1201 aatgcggact gttttgataa ataagtaatc gtaaacgatg aaaataatag gctgcggtat
     1261 ttttattaac gcct
//
LOCUS       DAWHRA010000233         1212 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWHRA010000233 DAWHRA010000000
VERSION     DAWHRA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.233"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..537
                     /locus_tag="WIB30_002082"
     CDS             <1..537
                     /locus_tag="WIB30_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3798079.1"
                     /translation="PFTDGNGRTGRILNSLLLIDKGLLDLPILYLSRYIIENRADYYR
                     LLLGVTERQDWESWIIYILDGVADTADWTVAKIDAIRRLFEQTRQHIRTHAQGIYTHE
                     LVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELSDIGVLQEIAIGRDKLFIHPRLME
                     LLRGEGNSFTSFQSLVKA"
     gene            567..>1212
                     /locus_tag="WIB30_002083"
     CDS             567..>1212
                     /locus_tag="WIB30_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3798080.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNRSVADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRM"
BASE COUNT          338 a          299 c          312 g          263 t
ORIGIN      
        1 ccgtttacgg acggcaacgg gcggacgggg cgcatattga acagcctgct attgattgat
       61 aaagggcttt tggatttgcc tattttgtat ttgagccgct atattatcga aaacagggcg
      121 gactattacc gcctgctttt aggcgtaacc gaacggcagg actgggaaag ctggataatc
      181 tacatcttag acggcgtagc cgataccgcc gattggacgg tagcgaaaat agatgcgata
      241 cgccgcctgt ttgagcagac acggcaacac atacggacac acgcacaagg aatctacacg
      301 cacgaactgg taaatcttct gtttgaacag ccatatacac gcattgccaa cctagaagcg
      361 gcagggatag ccaaacggca gacggcttct aagtatctga aagagctctc agacataggt
      421 gtgctgcaag aaatcgccat cggcagggac aaactattca ttcatccgcg cctaatggaa
      481 ctattgcggg gagagggcaa cagctttacc tcattccaat ccctcgttaa agcatagcca
      541 aaataatcaa taatccggag gtcaatatgg caagaaggtc aaaaacattt gaagaagctg
      601 ctgctgaggt tgaggaacgt ttcggtcatc gtggcattaa gttggtcgag tttgagggta
      661 cagccaagcc gtgtgtaatc aactgcccta aacatggaaa ccaaacctgt tcgaggtact
      721 ccaatatgtt cataggaagt agctggggtt gcccctcttg tggtaatgag caagctgcaa
      781 aagccggtat agcgaccctt aggaagaatc acatagcgtt agaaatgctg aaacaggctg
      841 taacaggtat gaccaagcaa gagcgcatca cgacgcaagc ctacaatgag atgaccaaat
      901 ccgtggcagg ttcaaacagc atagtcctta acgatgtcca aggcgatacg accatcaaca
      961 accatcatac gcatacgcac aaccgcagcg ttgccgacgg caaagcactg tcgatgaggc
     1021 tcacaccccg tcctttgttg tcagaccgtc aggcggcggc tttcgcccgt acaggcaaac
     1081 tcacgggcag tttcgacttg tttgcatcgg tggtcgcccc ttcacaatat acgtttgccg
     1141 ttgccatgcc cgacacgtcc atgtcgccgg ttatcgaaaa aggggatttg ctggtggtcg
     1201 agccgcgtat gc
//
LOCUS       DAWHRA010000234         1174 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWHRA010000234 DAWHRA010000000
VERSION     DAWHRA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.234"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(232..1086)
                     /locus_tag="WIB30_002084"
     CDS             complement(232..1086)
                     /locus_tag="WIB30_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ3798081.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIAAFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          335 a          349 c          305 g          185 t
ORIGIN      
        1 atcccgtcat tcccgcgcag gcggaaatcc ggacatccaa cgctgcagca atttgtcgga
       61 aataaccgaa actcaaaaaa ccggatgccc gcctgcgcgg gaatgacgac gcaaagatgg
      121 tcgttatttc ggataaatat ccgtcttttc gttccggata cccgctttcg caggaatgga
      181 gatggaaaga tttcagccta ttcggcaatc atcaaatacc gcttcccaac tttatttttg
      241 atgcgacagc cggccggcaa ccaaggtggc ggcgatgacg acaaacacgc ccaaggcgga
      301 cacgggcgac aggtgttcgc ccaaaatcaa aaccgccagc agcacgccga cgacgggttc
      361 gagcgaaatc aacagtcccg aaacattggc aggaacacgg ctcatcccct tgttccacag
      421 ccaataggcg taccagccgc accccaaacc caaatacagc agcgacaata ccatcccgac
      481 gctccagtcc acggtataac tttgcgccaa agcaagcgaa aacggcaggc acatcaacga
      541 tgcggcggca atggaaacag atgtgaatgc cggtgcgccg atgcgtgcaa tcagcctttg
      601 cgtcggacgc atagcggcac aaaagcccgc gcccgccaac aacaccagca ggcagccgaa
      661 ccagccgact tcgccgccct cttccgcacc gcccgccatc agcagcgcga caccggcaaa
      721 tgccgccgcg ccgcatatcc agtggtaggc acgcgctttg tcgttgaaga aaaagtgtcc
      781 gacaaacacc atcagcagtg gctcgagtcc gacaatgacc gatgcgctgg cggcggaagt
      841 gtatttcaac ccgacaaact gaagcagcag ggtcagcaca tagttgacaa acgccgcaat
      901 cagcaacggc ttccattcct cacgcggaat cttgccgaca tgacggcggc aggcgggcag
      961 tgcaggcagc gcggcaatta gcaggcgcac gccgaccatc aatgcgggat cgatgccgcc
     1021 atagacatat ttggcggcaa taaacgagct gctccagata atcagggcaa ggatttggta
     1081 aaacatagtg agtgataaag tcagatggtc atacaaaagt atagcggatg aacaaaaatc
     1141 aggacaaggc gacgaagccg cagacagtac aaat
//
LOCUS       DAWHRA010000235         1112 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWHRA010000235 DAWHRA010000000
VERSION     DAWHRA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.235"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..534
                     /gene="mafB"
                     /locus_tag="WIB30_002085"
     CDS             <1..534
                     /gene="mafB"
                     /locus_tag="WIB30_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ3798082.1"
                     /translation="HPNITATAQTALAVAEAATTVWRGKKVELNPTKWDWVKNTGYET
                     PAVRHMQTMDGEMAGGNRPPKSITSNGKADASTQPSLQAQLIGEQISSGHAYNKHVIR
                     QQEFTDLNINSPADFARHIENIVANPSESKKLSNGRSAYWDDKSGTVVIRNPKDPDGG
                     TAFRPKIGKQYFDDKLK"
     gene            547..756
                     /locus_tag="WIB30_002086"
     CDS             547..756
                     /locus_tag="WIB30_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798083.1"
                     /translation="MKLIGFEENLVKISLNKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLSLDKELKKIYDTWDKC"
     gene            865..1098
                     /locus_tag="WIB30_002087"
                     /pseudo
     CDS             865..1098
                     /locus_tag="WIB30_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          394 a          178 c          237 g          303 t
ORIGIN      
        1 catccgaata taactgcaac agcccaaact gcccttgccg tagcagaggc cgcaactacg
       61 gtttggcgcg gtaaaaaggt agaacttaac ccgaccaaat gggattgggt taaaaatacc
      121 ggctatgaaa cacctgctgt tcgccatatg cagactatgg atggggagat ggcaggtggg
      181 aatagaccgc ctaaatctat aacgtccaac ggcaaagcag atgcttccac acaaccgtct
      241 ttacaagcgc aactaattgg agaacaaatt agtagtgggc atgcttataa caagcacgtc
      301 ataagacaac aagaatttac ggatttaaat atcaattcac cagcagattt tgctcggcat
      361 attgaaaata ttgttgccaa tccttccgaa agcaaaaaat tatccaatgg gcgtagtgca
      421 tattgggacg ataagtctgg aacggttgta attagaaatc ccaaagaccc agatggtggg
      481 acagcattta gaccaaaaat aggtaaacag tattttgatg acaaattaaa gtaaaaggaa
      541 ttattcatga aattaattgg ttttgaagag aatttagtta aaattagtct caataaagat
      601 gagttgtata ttattcaagc aattgtagga gaaatttatt caggcgtatg cgtggattgt
      661 agagattttg agataattca tggtgttgaa aaaaataaag ttctttcttt ggataaagaa
      721 cttaaaaaaa tatatgatac ttgggataaa tgttagctag gtagggtggg cactcgttgc
      781 ccaccttttg ccgtatatta aaaatccttt tcaggctgga actgagtggg catcaatgcc
      841 aaccctaatt gtgctgatga agcagtatta agacaagcat cgaatggtga ttatcatggt
      901 tttccgcaat cagtagatgc tttttctgaa aatggaacag taatacaaat agttggcgga
      961 gataatattg taaggcataa actttatatc cctggatctt ataaaggaaa agatggaaat
     1021 tttgaatata tcagagaagc agatggaaaa attaatcata gattatttgt ccctaaccaa
     1081 caacttcctg aaaaatagta gatagagatt aa
//
LOCUS       DAWHRA010000236         1091 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWHRA010000236 DAWHRA010000000
VERSION     DAWHRA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.236"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            47..847
                     /locus_tag="WIB30_002088"
     CDS             47..847
                     /locus_tag="WIB30_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ3798084.1"
                     /translation="MNPAPKKPSLLFSSLLFSSLLFSSLLFSSAAQAASEDGSRSPYY
                     VQADLAYAAERITHDYPKPTGANNTSTVSDYFRNIRAHSIHPRVSVGYDFGGWRIAAD
                     YASYRKWNNNKYSVNIKKGRETQHNREELKTENQENGTFHAASSLGLSAIYDFKLNDK
                     FDKFKPYIGARVAYGHVKHQVHSVETKTTIVTSKPNGGPVPSSVTKPPYHQSHSISSL
                     GLGVIAGVGFDITPKLTLDTGYRYHNWGRLKNTRFKTHEVSLGMRYHF"
BASE COUNT          298 a          319 c          222 g          252 t
ORIGIN      
        1 aaaatctttc ttttttaatc agttaaaacc gaatacggag tcgaaaatga atccagcccc
       61 caaaaaacct tctcttctct tctcttctct tctcttctct tctcttctct tctcttctct
      121 tctcttctct tccgcagcgc aggcggcaag tgaagacggc agccgcagcc cgtattatgt
      181 gcaggcggat ttagcctacg ccgccgaacg cattacccac gattatccga aaccaaccgg
      241 tgcaaacaac acaagcacag taagcgatta tttcagaaac atccgtgcgc attccatcca
      301 ccctcgggtg tcggtcggct atgatttcgg cggctggagg atagcggcag attatgccag
      361 ttacagaaaa tggaacaaca ataaatattc cgtcaacata aaaaagggga gagaaaccca
      421 gcacaacagg gaagaactga agacggaaaa tcaggaaaac ggtacattcc acgccgcttc
      481 ttctctcggc ttatccgcca tttacgattt caaactcaac gataaattcg ataaattcaa
      541 accctatatc ggtgcgcgcg tcgcctacgg gcacgttaaa catcaggttc attcggtgga
      601 aaccaaaacc acgattgtta cctctaaacc aaatggaggc cctgtcccaa gttcggttac
      661 caaacctccc tatcaccaaa gccacagtat cagcagcttg ggtcttggtg tcatcgccgg
      721 tgtcggtttc gacatcacac ccaagctgac cttggacacc ggataccgct accacaactg
      781 gggacgcttg aaaaacaccc gcttcaaaac ccacgaagtc tcattgggca tgcgctacca
      841 cttctgattc cccgataccg atgccgtctg aaccttcaga cggcattttt gattcacctg
      901 ccgttgacaa acgcggggcg ggcgtggaaa tacccgaacc gtcattcccg acaacaccgt
      961 aatcttgaaa cccgtcattc ccgcgcaggc gggaatctag acctgtccgc acagaaactt
     1021 atcggataaa aacagttgcc caaaccccgc gttctagatt cccactttcg tgggaatgac
     1081 gattcagttt c
//
LOCUS       DAWHRA010000237         1077 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWHRA010000237 DAWHRA010000000
VERSION     DAWHRA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.237"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..406
                     /locus_tag="WIB30_002089"
     CDS             <1..406
                     /locus_tag="WIB30_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ3798085.1"
                     /translation="SLITFAPETEESGLQVLTATANGYGKRTPIADYSRKNKGGQGNI
                     AINTGERNGDLVAATLVGETDDLMLITSGGVLIRTKVEQIRETGRAAAGVKLINLDEG
                     ETLVSLERVAEDESELSDASVISNVTEPEVEN"
     gene            563..967
                     /locus_tag="WIB30_002090"
     CDS             563..967
                     /locus_tag="WIB30_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE transposase"
                     /protein_id="HEZ3798086.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGKVEADESYFAERQNHINGIGNFWNRTKRHLRKFNGIPKAHF
                     ELYLKGYERRFNNSEIKVQISILKQLVKSSLS"
BASE COUNT          352 a          240 c          228 g          257 t
ORIGIN      
        1 cagcctgatt accttcgccc ctgaaaccga agaaagcggt ttgcaagttt taaccgccac
       61 cgccaacgga tacggaaaac gcaccccgat tgccgattac agccgcaaaa acaaaggcgg
      121 gcaaggcaat attgccatta acaccggcga gcgaaacggc gatttggtcg ccgcaacctt
      181 ggtcggcgaa accgacgatt tgatgctgat taccagcggc ggcgtactta tccgcaccaa
      241 agtcgaacaa atccgcgaaa ccggccgcgc cgcagcaggc gtgaaactga ttaacttgga
      301 cgaaggcgaa accttggtat cgctggaacg tgttgccgaa gacgaatccg aactctccga
      361 cgcttctgta atttccaatg taaccgaacc ggaagtcgag aactgaaaat catctcccga
      421 tgccgtctga agattcagac ggcatttatt ttatccctca tccgtcatcc agcttctcac
      481 aatatagcgg attatagtca attaaaaaca aggggctgtc ctagataact agggaaattc
      541 aaattaagtt agagttgccc ctatgagaaa aagtcgtcta agccagtata aacaaaataa
      601 actcattgaa ctgtttgtcg caggtgtaac tgcaagaacg gcagcagagt tagtaggcgt
      661 taataaaaat accgcagcct attattttca tcgtttacga ttacttattt atcaaaacag
      721 tccgcatttg gaaatgtttg acggcaaagt agaagcagat gaaagttatt ttgctgaacg
      781 acaaaaccat atcaatggaa ttgggaactt ttggaaccgg acaaaacgtc atttacgcaa
      841 gtttaacggc attcccaaag cgcattttga gctgtattta aaggggtacg aacggcgttt
      901 taacaacagt gagataaaag ttcaaatttc cattttaaaa caattagtaa aatcgagttt
      961 atcctagtta tctaggacag ccccaaaaac aaaatagtac aatattcaac tttgaaggtc
     1021 taaccatggc atactctgcg gacttaagaa acaaagcttt aaactatagt ggattaa
//
LOCUS       DAWHRA010000238         1069 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWHRA010000238 DAWHRA010000000
VERSION     DAWHRA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.238"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..392
                     /locus_tag="WIB30_002091"
     CDS             <1..392
                     /locus_tag="WIB30_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ3798087.1"
                     /translation="PVAASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVA
                     IVCKVLLNEARAGMVSSITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIV
                     FIVALLVLLVILIQSTGNTLARKLDKR"
     gene            551..>1069
                     /locus_tag="WIB30_002092"
     CDS             551..>1069
                     /locus_tag="WIB30_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798088.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGI"
BASE COUNT          252 a          338 c          274 g          205 t
ORIGIN      
        1 gcccggttgc cgcctcgctg gtgttgagcg tgtcgggatt gttttatttt gcccgactgg
       61 tggaacaaaa cctgcgcgaa gtccccaaag gcgtgattga agccgccgcc gcgatgggtg
      121 cgccgccggt tgccatcgtc tgcaaagtcc tcttgaacga agcgcgcgcg ggcatggttt
      181 ccagcattac cgtgcttgcc atcgggcttt tgtcatacag cgcggcggca gggatgatag
      241 gcggcggcgg cttgggcgac ctcgccatcc gctacggcta ctaccgctac caaaccgaag
      301 tcatcgtctt catcgtcgcc ctcctcgtgc tgctggtcat cctgattcaa agcaccggca
      361 acacgttggc gcggaaactc gacaaacgtt aaacccgaat gccgtctgaa cgccaaaacc
      421 cccaccgcta tccgaaaaat gctataaaat tcccctgttc gcggcaaatg ccgtctgaac
      481 gccgaatccg gacggcagga ctccctgccc gtcatttttg tttgaaaccg ccacaacatc
      541 aggagaaaat atgaaaacct tcttcaaaac cctttccgcc gccgcactcg cgctcatcct
      601 cgccgcctgc ggcggtcaaa aagacagcgc gcccgccgca tccgcttctg ccgccgccga
      661 caacggcgcg gagaaaaaag aaatcgtctt cggcacgacc gtcggcgact tcggcgatat
      721 ggtcaaagaa cacatccaac ccgagctgga gaaaaaaggc tacaccgtca aactggtcga
      781 gtttaccgac tatgtacgcc cgaatctggc attggctgag ggcgagttgg acatcaacgt
      841 cttccaacac aaaccctatc ttgacgactt caaaaaagaa cacaatctgg acatcaccga
      901 agtcttccaa gtgccgaccg cgcctttggg actgtacccg ggcaagctga aatcgctgga
      961 agaagtcaaa gacggcagca ccgtatccgc gcccaacgac ccgtccaact tcgcccgcgt
     1021 cttggtgatg ctcgacgaac tgggttggat caaactcaaa gacggcatc
//
LOCUS       DAWHRA010000239          995 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWHRA010000239 DAWHRA010000000
VERSION     DAWHRA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.239"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..851
                     /locus_tag="WIB30_002093"
     CDS             <1..851
                     /locus_tag="WIB30_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEZ3798089.1"
                     /translation="AALCGFFAEFEDIIGNGGSLTEIDVDTMLYRHRSVRPGFISLSF
                     DTIAGFNANGALPHYSATPESHSTISGNGLLLIDSGAQYKGGTTDITRVVPVGTPTAE
                     QKRDNTLVLKAHIALAEAVFPENIPSPLIDAICRKPLWQAQCDYGHGTGHGVGYFLNV
                     HEGPQRIAFAAPATPETAMKKGMVTSIEPGLYRPGKWGIRIENLAANQAVATPQETEF
                     GSFLCFETLTLCPIDTRLMDTALMTDGEIDWVNRYHAEVRRRLEPLTEGAAKAWLIKR
                     TEPLAR"
BASE COUNT          240 a          354 c          242 g          159 t
ORIGIN      
        1 gcgcggcgtt gtgcggtttc ttcgccgagt ttgaagacat catcggcaac ggcggcagcc
       61 tgaccgaaat cgacgtggac accatgcttt atcgccaccg cagcgtgcgc ccgggcttca
      121 tttcattgag tttcgacacc atcgcgggct tcaacgccaa cggagcactg ccgcattaca
      181 gcgcaacgcc cgaaagccac agcaccatca gcggcaacgg gcttttgctc atcgactccg
      241 gcgcgcaata caaaggcggc acgaccgaca tcacccgcgt cgtccctgtc ggcacaccca
      301 ccgccgaaca aaaacgcgac aacaccctcg ttctcaaagc ccatatcgcg cttgccgaag
      361 ccgtgttccc cgaaaacatc ccctcgccgc tgattgatgc gatttgccgc aaacccctgt
      421 ggcaggcgca atgcgactac ggccacggca ccggacacgg cgtaggctat ttcctcaacg
      481 tccacgaagg cccgcagcgc atcgccttcg ccgcccccgc cacgcccgaa accgccatga
      541 aaaaaggcat ggttacctcc atcgaacccg gactctaccg cccgggaaaa tggggcatcc
      601 gcattgaaaa ccttgccgcc aaccaagccg tcgccacccc tcaagaaacc gaattcggca
      661 gcttcctctg ttttgaaacc ctgaccctct gccccatcga tacacgcctg atggacaccg
      721 ccctcatgac cgacggcgaa atcgactggg tcaaccgcta ccacgccgaa gtccgccgcc
      781 gcctcgagcc gctgaccgaa ggcgcggcaa aagcgtggct gatcaaacgc accgaaccgc
      841 tggcgcgtta aacagcacgg cgcaaaaaat gccgtctgaa agcccttcag acggcattgg
      901 tttcccaaaa catcccgcac cgttttcatc ttgccgcaag caaatatagt ggattaacaa
      961 aaatcaggac aaggcgacga agccgcagac agtac
//
LOCUS       DAWHRA010000240          994 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWHRA010000240 DAWHRA010000000
VERSION     DAWHRA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.240"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            15..197
                     /locus_tag="WIB30_002094"
                     /pseudo
     CDS             15..197
                     /locus_tag="WIB30_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(356..>994)
                     /locus_tag="WIB30_002095"
     CDS             complement(356..>994)
                     /locus_tag="WIB30_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ3798090.1"
                     /translation="INRQLTEKGLKVEKASAAVVDATIIQTADSKQRQAIEVDEEGQI
                     SGQTTPSKDKDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLPPLLE
                     GIAEGTTVYADKGYDSAENRQHLEEHQLQDGIMRKACRNRPLSETQTKRNRYLSKTHY
                     VVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT          219 a          232 c          247 g          296 t
ORIGIN      
        1 taaaaaatat ccctttgaaa gattttgatg aagtttgtga aaagtgggag aaatccttta
       61 aagaaaatgg aaaagaaatt gaatataatc aaaaatattc aagaatttat ggtgaagttc
      121 gtggagctgg ctttaatggc gatatcggag aaaagctaca tcgttgtgcc gtgatggaat
      181 gggctgaaaa tgagaccgag acctttgcaa aattcctttc cctcccgaca gccgaaaccc
      241 aaacacaggt tttcagctgt tttcagctgt tttcgcccca aatatctcct aattctaccc
      301 aaataccccc ttaatcctgc ccggatacct gataatcagg catccgggct gctttttagg
      361 cggcagcggg cgcacttaac ctgttggccg ctttcaacag gttcaaacac atcgccttca
      421 ggtggctttg cgcactcact ttaatcagtc cgaaataggc tgcccgcgca tagcggaatt
      481 tacggtgcag cgtaccgaag ctttgttcga ccacataatg ggtcttcgac aaataccggt
      541 tgcgtttggt ttgcgtttcc gacagcggac ggttgcggca ggctttgcgc ataatgccgt
      601 cctgcaactg atgttcttcc agatgttgcc ggttttccgc actgtcatag cctttgtcgg
      661 catagacggt cgtaccttca gctatccctt ctaacaacgg cggcaggtgt ttgcactcat
      721 gggcattggc gggggtgatg tgcagtttct cgatatagcc ttccgcatcg gtgcgggtat
      781 gttgtttgta accgagtttg tagaggccgt ttttctttat ccaacgggca tctttgtcct
      841 tactcggtgt ggtttggccg ctgatttgtc cttcttcatc gacttctata gcctggcgct
      901 gtttgctgtc ggcggtctga ataatggtgg cgtcaacaac ggcggcggat gctttctcta
      961 cttttaggcc tttttcggtc agttgtcggt tgat
//
LOCUS       DAWHRA010000241          987 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWHRA010000241 DAWHRA010000000
VERSION     DAWHRA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.241"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..234)
                     /locus_tag="WIB30_002096"
     CDS             complement(<1..234)
                     /locus_tag="WIB30_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ3798091.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSG"
BASE COUNT          334 a          278 c          186 g          189 t
ORIGIN      
        1 gcctgaaacg gcttctgccc gccattgcga agacagtgcc gcttccgttc tgaatacgcc
       61 gtaagccgcg cccgccaaca cgcacagcat cagaccggca aacgcaaaac gccttgcaaa
      121 cacagcaaaa accgcaaaag ccgccagcca aaacagccaa tgcggcacga ccggcagcgc
      181 aaacgaagcc gccgcgccga caacccaaca cggcaaaaac caacgacgga acacgtctcc
      241 ccaccccaga tatttcaaaa ataaacggat aataaagcaa atccgaaaaa ccgtcataac
      301 aagatttgaa aatgatatag tggtttaaat ttaaatcagg acaaggcgac gaagccgcag
      361 acagtacaag cagtacgaca aggcgaggca acgctgtact ggtttttgtt aaaccactat
      421 aagaatgcaa acgaaaagcc tcagtattat ccgagacaac cgcaaacttt ccatcgtcat
      481 tcccacgaac ccacatcccg ttattaccac gaaagcggga atctaggact cagggttaag
      541 aaaacctaca tcccgtcatt ccctcaaaaa cagaaaacca aaatcagaaa cctaaaatcc
      601 cgtcattccc acgaaagtgg gaatctaggt ctgtcggtgc ggaaacttat cggataaaac
      661 ggtttcttta gattccacgt tctagattcc cgcctgcgcg ggaatgacga ttcataagtt
      721 tcccgaaatt ccaacataac cgaaacctga cagtaaccgt agcaactgaa ccatcattcc
      781 caccactttt cgtcattccc acgaaagtgg gaatctagaa tctcggactt tcagataatc
      841 tttgaatatt gccgctgcct taaggtctag attcccgcct gcgcgggaat gacggcagag
      901 cggtttctgt tgctcccgat aaattcctaa aactcaaaat ttcatcattc ctacaaaaac
      961 agaaaaccaa aatcagaaac ctaaaat
//
LOCUS       DAWHRA010000242          984 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWHRA010000242 DAWHRA010000000
VERSION     DAWHRA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.242"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            78..>984
                     /locus_tag="WIB30_002097"
     CDS             78..>984
                     /locus_tag="WIB30_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ3798092.1"
                     /translation="MNSQKLPEVKNGNYQGKNRQVTFNAYFQGTKKNSIFSFDNTDEV
                     VAQKGGTVVFGAATYLPPYGKVSGFDADKLQERGNAVGWIGTIHPGLVGYSYQGSTCN
                     SRDCPELSYKTQFIFSDQGLKNKVNGKLDIDADKSRDNSPIYKLPDHPWLGVSFNLSS
                     ESTNESKQLKKLVSSFSENVTQNNSTQSPYTDINLVYTTDKHRNNGNIGRGDNHAVAF
                     YLNAKLHLLAKKQIKGISKDQTINLGTLKTRIKPSDAWKNKRHHVINVNNWTFEDKGS
                     VSVKLKLPEVKAGRCINKPNPNPNKK"
BASE COUNT          312 a          226 c          231 g          215 t
ORIGIN      
        1 ccgccatatt gatgttttcc cataccgggg gggggggggg gcaggcgcaa gcgcaaacgc
       61 attcatacac tattgtaatg aacagtcaaa agctgcccga ggtaaagaat gggaactacc
      121 aaggcaagaa ccgccaagtt acctttaatg catattttca gggaactaaa aaaaactcta
      181 ttttctcatt cgataatacc gatgaagttg ttgctcaaaa aggtggtact gtcgttttcg
      241 gcgcggcgac ctacctgccg ccctacggca aggtttccgg ttttgatgcc gataagctgc
      301 aagagcgcgg caatgccgtt ggttggattg gtacgatcca cccggggttg gtaggctaca
      361 gctaccaagg tagcacatgc aacagcagag actgtcctga acttagctat aaaacccaat
      421 ttatcttcag tgatcaaggg ttgaaaaaca aggtaaacgg caagctggat atagacgcag
      481 acaaaagccg cgacaattcg cccatttata aattgccgga tcatccttgg ttgggcgtgt
      541 ctttcaattt gagcagcgag agcaccaacg aatcaaagca actcaagaaa ttagtatctt
      601 cttttagcga gaatgtgacg cagaacaata gtacgcaaag cccatacaca gacataaacc
      661 tcgtgtatac gacggacaag caccgtaata atggtaatat cggtcgtggc gacaaccacg
      721 ccgtcgcctt ttatctgaac gccaagctgc acctgctggc taaaaaacag attaaaggta
      781 tctcgaaaga ccaaacaatt aatttgggta ccttgaaaac gcgcatcaag ccgtcggacg
      841 cgtggaagaa taagaggcat cacgttatta atgttaataa ttggacgttt gaagataaag
      901 gatcagtcag cgtcaaactc aaattgccgg aagtcaaagc aggccgctgc atcaataaac
      961 cgaaccccaa tcccaacaaa aaag
//
LOCUS       DAWHRA010000243          982 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWHRA010000243 DAWHRA010000000
VERSION     DAWHRA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.243"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(464..>982)
                     /locus_tag="WIB30_002098"
     CDS             complement(464..>982)
                     /locus_tag="WIB30_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3798093.1"
                     /translation="PFTDGNGRTGRILNSLLLIEKGLLDLPILYLSRYIIENRADYYR
                     LLLGVTERQDWESWIIYILDGVADTADWTVSKIDAIRRLFEQTRQHIRTHAQGIYTHE
                     LVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELSDIGVLQEIVIGRDKLFIHPRLME
                     LLRGEGNSFTSF"
BASE COUNT          242 a          213 c          227 g          300 t
ORIGIN      
        1 taggtttcca tgcggacgcg ttcggattca cgactgcgcg gaaatgacgg gattttggtg
       61 tattccctaa aaaaataaaa aaacatttgc aactttgtta aaaataaagg ctgtgtttta
      121 acgatgtgtt gatatttaat tttagaaagg tagctattta atagttacct tttcttattt
      181 aaaaatagct ttctcaaatt ccatgaacgc ctcaatacga tatgcagatg ctctatcgaa
      241 attaagtttc aacattttgt ttattaaaca ttttatttta gccatttttc aatatacccc
      301 caaatatacc cccaatttgc acaagtcaaa attttaggca ggggttttgt tgctcccatg
      361 atacgcgggc ggatgccggc ttttcgcggg ggaaatacaa ggggtctcgg ttcgggtgtc
      421 aaaatccctg tttcgtgtta gtcatgtggg ggggaagagg gggttagaat gaagtaaagc
      481 tgttgccctc tcctcgcaat agttccatta ggcgcggatg aatgaatagt ttgtccctgc
      541 cgatgacgat ttcttgcagc acacctatgt ctgaaagctc tttcaggtac ttagaggccg
      601 tctgccgttt ggctatccct gccgcttcta ggttggcaat gcgtgtatat ggctgctcaa
      661 acagaagatt taccagttcg tgcgtgtaga ttccttgtgc gtgtgtccgt atgtgttgac
      721 gtgtctgctc gaacaggcgg cgtatcgcat ctattttcga taccgtccaa tcggcggtgt
      781 cggctacgcc gtctaagatg tagattatcc agctttccca atcctgccgt tcggttacgc
      841 ctaaaagcag gcggtaatag tccgccctgt tttcgatgat gtagcggctc aaatacaaaa
      901 taggcaaatc caaaagccct ttttcaatca atagcaggct gttcaatatg cgccccgtcc
      961 gcccgttgcc gtccgtaaac gg
//
LOCUS       DAWHRA010000244          978 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWHRA010000244 DAWHRA010000000
VERSION     DAWHRA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.244"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            9..>978
                     /locus_tag="WIB30_002099"
     CDS             9..>978
                     /locus_tag="WIB30_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEZ3798094.1"
                     /translation="MLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLKNG
                     AAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTAFD
                     NSGDFVRLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTHID
                     DNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSAGVVGTPDKLVGESETVLKKF
                     VYECPMLNEILDKAVWETDFPFHSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSSGV
                     TIALHSARLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFRTYSGLN"
BASE COUNT          230 a          290 c          252 g          206 t
ORIGIN      
        1 aagtctgcgt gttggaaaaa cagcactttc cgcgcttcgt catcggcgaa agcctgctgc
       61 cgcactgtat ggaaatgctg gaagaagccg gatttgccga tgccgtgcgc gccgagcccg
      121 gctttcagtt gaaaaacggc gcggcgtttt cgtggggcag tcgttatacc gaattcgatt
      181 tcaccgataa attttcagac ggccccggca cgacttatca agtacgccgc gccgtgttcg
      241 ataaaatcct gattgaagaa gccgccaaac aaggcgttga agtacgtttc gggcacggcg
      301 taaccgcgtt cgacaacagc ggcgattttg tccgcctgaa catcgaaacc gacaccggca
      361 agcgctatga actgaccgcg aaattcgtct tggacgcaag cggttacgga cgcgtgctgc
      421 cgcgcctgct aaacttggaa acgccctcgc acctgccgcc gcgccaagcg catttcacgc
      481 acatcgacga caacatcacc aacccgaaat tcgaccgcaa caaaatcctg attaccacct
      541 atccgcaaca ccgcgacgtg tggatttggc tgattccctt cggcgacaac cgctgttccg
      601 ccggcgtggt cggcacgccc gacaaacttg tcggcgaatc ggaaacagtg ttgaaaaaat
      661 ttgtttacga atgcccgatg ctgaacgaaa ttttggacaa agccgtttgg gaaaccgatt
      721 ttccgttcca ctccatccaa ggctattccg ccaatgtgaa atcgctgcac ggcaggcatt
      781 tcgcgctatt gggcaatgcc gccgagttcc tcgatcccgt gttctcgtcg ggcgtaacca
      841 tcgcgctgca ctccgccaga cttgccgccg atctgttggc aaaacaactc aaaggcgaag
      901 ccgccgattg gcaaaccgaa tttgccgaac ccttgatgat cggcgtggac gcgttccgca
      961 cctatagtgg attaaatt
//
LOCUS       DAWHRA010000245          970 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWHRA010000245 DAWHRA010000000
VERSION     DAWHRA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.245"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..881)
                     /gene="mafB"
                     /locus_tag="WIB30_002100"
     CDS             complement(<1..881)
                     /gene="mafB"
                     /locus_tag="WIB30_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ3798095.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWENDPNKNW
                     WANRMDDIRGIVQGAANPFLTGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAASLLQDSAFAV"
BASE COUNT          230 a          241 c          219 g          280 t
ORIGIN      
        1 accgcaaaag cactgtcttg taataggctc gcggccgcaa gttgtgcttc cggacttaaa
       61 tttcctaaat tgtgaatacc ctgtaaagtt ttccgtgctg ccgcataggt taccgggttt
      121 accgcactgt ctgtaattgc cccaactccc aatccctgaa aacccgttag aaaaggattg
      181 gctgcacctt ggacgatgcc gcgaatatca tccatacggt tagcccacca atttttattg
      241 gggtcgtttt cccatatcag ttttcctgct tcatccgcac ggctgaaaaa accagaggcg
      301 gcaccggcat tttcttttag ccaacggtct gaaaatgggg cttgagggac aatattagtc
      361 tttgtttttg ttgaagttcc tttgacataa tagctgtata tatcccttgc tcctccgggg
      421 ggcggataat cgctgccttg cggtccgtca tatccatcct ccggatggat ttccgaccct
      481 gttcgatgaa gttggtaaac agtaaaaccg ccgtctacac cgccgctgaa atcagaagtg
      541 ctttttgaat catgatgatc gaacggactg tgtacttcat gtccgtgccc tgaaaaatgg
      601 gtttcataac cgataacacc ttcaaaccct gtccgttcgt gtgtaatagg cagtacagga
      661 ctgaccgcag ttgcatcaaa tgtctggacg gcgtaaaccc gatttttaac actgccgcga
      721 ccatcgccga acagatggta tttgccaccc ggttcgtagt gttttgcctg catatcatcg
      781 cgcaaacggg catccaaacc gttcgcatga ctaatgggga tttgcagcaa cgatattgct
      841 gctgcaaaca gcatcatgaa tttttgaata ggcaaattca ttttgttttc cttggtagca
      901 agcggttatg gtagtgtgaa tcaaggttgc ccttgtctat gttgtcgcac tacttcatcg
      961 ctgtatccat
//
LOCUS       DAWHRA010000246          945 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWHRA010000246 DAWHRA010000000
VERSION     DAWHRA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.246"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(15..206)
                     /locus_tag="WIB30_002101"
     CDS             complement(15..206)
                     /locus_tag="WIB30_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798096.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            335..895
                     /locus_tag="WIB30_002102"
     CDS             335..895
                     /locus_tag="WIB30_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ3798097.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          263 a          266 c          188 g          228 t
ORIGIN      
        1 cgtaccggtt tttgttaatc cgctatacca cgatgaatcc ttcgcaatat ctgtttatcg
       61 acctcaattt tgacaaaata ccggatacgc gcctttgttg cttttccatc ttccaaccaa
      121 ctgtaaatct caaacagccg gtacacgcca tgcttcagtt tcttttcctg tcggcggatt
      181 gtttcgacaa agaattgaaa atccatttca tgcaccttaa aatttaatct gcattcaaac
      241 cttttcactt tggaagcacc atttatcgga tgtcccttcg caataaacaa attttcccga
      301 taccgccgcc catttcaacc caaacccaaa acctatgaaa aacctcatcg ccttcaacaa
      361 accctatggc gttatctgcc aattttcacc gcacgaaaaa cacaaaagcc tcaaagactt
      421 tatcaatctt cccggcttct accccgccgg acggctcgac accgacagcg aggggctgct
      481 gctgctgacc gacgacggca ggcttcaggc acaaattacc gaccccaaat tcaaacaccc
      541 caaaacctac tgggcgcaac tggagggcgt acccgacgaa agccgattgg aaagcctaag
      601 aaaagggata gacttaggcg gtttcgttac ccgtccggca agcatccgca tcttgaaaca
      661 cggagaagca gattcgttat gggagcgcat cccgccgata cgcgtccgca aaaccgttcc
      721 cgatttttgg attgaaatta ccatttctga gggcaaaaac cgccaagtca ggcgaatgac
      781 cgccaaggcg ggctatccct gcctgcgtct gatcagagtg gcaagcggca ggctgaaact
      841 gtttgatttg gatttaaaac ccggggaatg ggcatacgcc ccgtttaaac cataatcacg
      901 tttatctcat catttccaca aaagtgggaa tccggaattt tatag
//
LOCUS       DAWHRA010000247          914 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWHRA010000247 DAWHRA010000000
VERSION     DAWHRA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.247"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            32..>914
                     /locus_tag="WIB30_002103"
     CDS             32..>914
                     /locus_tag="WIB30_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ3798098.1"
                     /translation="MNEQNQLKVKQKDSYSTLREKDRERKFIYNKGRQGGGSVFFDNT
                     DTLVSRQSGTAVFGTATYLPPYGKVSGFDANALKERNNAVDWIHTTRAGLAGYAYTNV
                     ICRSHQCPQLVYKTRFSFDNPDLAKRGGGLDRHTEPSRDNSLIYKLKDHPWLGVSFNL
                     GSENTVKNSQSSSRLISSFSEDNNNQTIVSTTENHPISLGDGQREHTAVAYYLNAKLH
                     LLDKKGIKDITGKTVRLGVLKPSIDVKTQRTGLGGLLDFHAKWDIKDTGQIPVELGLP
                     QIKAGRCINKPNPNPKAQ"
BASE COUNT          271 a          239 c          214 g          190 t
ORIGIN      
        1 gcaggcggaa acccataact acgctattgt catgaacgaa caaaatcagc tgaaggtaaa
       61 gcagaaggat tcatattcaa ccttaaggga aaaagacagg gaacgcaaat ttatctataa
      121 taaaggccgg cagggtggag gctctgtctt tttcgacaat accgataccc ttgtttcccg
      181 acaaagcggt actgccgttt ttggcacagc cacctacctg ccgccctacg gcaaggtttc
      241 cggttttgat gccaacgctc tgaaagagcg caacaatgcc gtcgattgga ttcataccac
      301 ccgggccggg ctggcaggct acgcctacac caacgtcata tgcagaagcc accaatgccc
      361 ccaacttgtc tataaaaccc gattttcctt cgataatccc gacttggcaa aaagaggagg
      421 cgggctggat aggcacacag agccaagccg cgacaattcg ctcatttaca aattgaagga
      481 tcatccatgg ttgggcgtat ctttcaattt gggcagcgag aataccgtca aaaatagcca
      541 atcatccagc agattgatat cttcttttag tgaagacaat aataatcaaa ccatcgtctc
      601 tacgacagaa aaccacccta tttcccttgg cgacgggcag cgcgaacata ccgccgtggc
      661 ctattatctg aacgccaaac tgcacctgct ggataaaaaa gggattaaag atatcaccgg
      721 caaaacagtg cggttgggtg tcttgaagcc gagcatcgat gtgaagacac aaaggacggg
      781 gcttggcggc ttgctagatt ttcatgctaa gtgggacatt aaagataccg ggcagattcc
      841 ggtcgagctc ggcctgccgc aaatcaaagc aggccgctgc atcaacaaac cgaaccccaa
      901 tcccaaagcc caag
//
LOCUS       DAWHRA010000248          908 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWHRA010000248 DAWHRA010000000
VERSION     DAWHRA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.248"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(168..868)
                     /locus_tag="WIB30_002104"
                     /pseudo
     CDS             complement(168..868)
                     /locus_tag="WIB30_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          180 a          240 c          229 g          259 t
ORIGIN      
        1 attgcgttaa agtttaaatt gagacctttg caaaaaagcc cttcccccaa cagccgaaac
       61 ccaaacacag gttttcgtct atttccgctc ctaattactc ctgattttac ccaaatgccc
      121 ccttaatcct ccccggacgc cccataatca ggcatccggg ccgcctttta ggcggcagcg
      181 ggcgcactta gcctgttggc cgctttcaac aggttcaaac acatcgcctt caggtggctt
      241 tgcgcactca ctttaatcag tccgaaatag gctgcccgag cgtagcggaa tttacggtgc
      301 agcgtaccga agctctgttc gaccacataa cgggtcttcg acaaatatcg gttgcgtttg
      361 gtttgtgcct ccgtcagcgg acggttgcgg caggctttgc gcataatgcc gtcctgcaac
      421 tgatgttctt ccagatgttg ccggttttcc gcactgtcgt agcctttgtc ggcatagacg
      481 gtcgtacctt cagctatccc ttccaacaac ggcgacaggt gtttgcactc atgggcattg
      541 gcgggggtaa tgtgcagttt ctcgatatag ccttcctcat cggtacgggt atgttgtttg
      601 taactgagtc tgtagaggcc gtttttcttt gtccggcggg catctttatc tttactcggt
      661 gtggtttggc cgctgacttt tccttcttca tcgacttcta tagcctgacg ctgtttgctg
      721 ccggcggtct gaataatggt ggcgtcaacg acagcggcgg atgctttctc tacttttaag
      781 cctttttcgg tcagttggca gttaatcagt ttgagcaatt cggacagggt gtcgtcttgc
      841 gccgccagtt gcggtagcgg cataaagtat agtggattaa caaaaatcag gacaaggcga
      901 cgaagccg
//
LOCUS       DAWHRA010000249          882 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWHRA010000249 DAWHRA010000000
VERSION     DAWHRA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.249"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(409..693)
                     /locus_tag="WIB30_002105"
     CDS             complement(409..693)
                     /locus_tag="WIB30_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ3798099.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          257 a          234 c          158 g          233 t
ORIGIN      
        1 cgcgggaatg acggtttaga agttgcccga aacctcaaaa aaaccgaaac cgaacaagcc
       61 ggattcccgc ctgcgcggga atgacggcag aacggtttcg gttttttccg ataaatgccg
      121 caatctcaaa tcccgtcatt cccgcgaacc tacatcccgt cattcccacg aaagtgggaa
      181 tctagaatct ctaaagcttc agctaacctt tgaatattac tgttgttcta aggtctagat
      241 tcccgcctgc gcgggaataa cgggtctttt ataaactttg aatattgccg ttatcccaag
      301 gtctagattc ccgcctacgc gggaatgacg gtttagaagt tgcccgaaac ctcaaaaaaa
      361 aaaccgaaac cgaacaagcc ggattcccgc ctgcgcggga atgacgggct aaataatatc
      421 aaaccataaa tcctgccaag aaacattatt ttcttcaatc agttgcaatt tccaagccct
      481 gttccatttc ttcaactgtt tttcccgagt aattgcactc tccatcgtag gatgcagttc
      541 ataccaaacc agcatagtaa cgttgtaccg tgatgtaaat ccctcaatca aatgctccct
      601 atgttggtaa atacgttgca ccaaatcaga tgtaacgcca atgtataacg tgccattacg
      661 ttggcttgct aaaatataaa ccgcaggctg catataatac ccttttgaat tatttcaatt
      721 tatattcccg cgaacaccat cccgtgatta ctttaaccct tcgttattcc catagctttc
      781 catcattccc gcaactcttc gtcattcccg cgaaagtggg aatctagaac gcaaaatcta
      841 aagaaaccgt tttacccgat aagtttccgc accgacaaac ct
//
LOCUS       DAWHRA010000250          874 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWHRA010000250 DAWHRA010000000
VERSION     DAWHRA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.250"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            102..503
                     /locus_tag="WIB30_002106"
     CDS             102..503
                     /locus_tag="WIB30_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ3798100.1"
                     /translation="MNTTRLPTALVLGCFCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKSKKN"
     gene            complement(566..856)
                     /locus_tag="WIB30_002107"
     CDS             complement(566..856)
                     /locus_tag="WIB30_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798101.1"
                     /translation="MAKYFTETGISVREHFDFFGEFVVSPAARSGDLALTYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVEGGRIDIGGFMLTAKEVDGGGNIGSMGLKVLR"
BASE COUNT          266 a          270 c          176 g          162 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctatactact gattcggcaa aaaaatcttt cacccgctac
       61 gcaaaatcaa caacaaggtt tcaaacatga aggaaacacc aatgaacaca acccgactgc
      121 cgaccgccct cgtcttgggc tgcttctgcg ccgccgcttc tgccgccgac aacagcatca
      181 tgacaaaagg gcaaaaagtg tacgaatcca actgcgtcgc ctgccacggc aaaaagggcg
      241 aaggccgcgg aaccatgttt ccgccgctct accgctccga cttcatcatg aaaaaaccgc
      301 aggtgctgct gcacagcatg gtcaaaggca tcaacggtac aatcaaagtc aacggcaaaa
      361 cctacaacgg attcatgccc gcaaccgcca tcagcgatgc ggacattgcc gccgtcgcca
      421 cttatatcat gaacgccttt gacaacggcg gcggaagcgt taccgaaaaa gacgtaaaac
      481 aggcaaaaag caaaaaaaac taaacagaca aaatgccgtc tgaaaccggc aatccggctt
      541 cagacggcat tcaaatcaaa cctttctaac gcagcacttt cagccccata gacccgatat
      601 tgccgccacc gtcaacctcc tttgcggtca gcataaagcc gccgatgtca atacggccgc
      661 cctcgaccgg ctcctgacta tcggaacgct tatcgaaaag ctcggcaagg ctcaaaccct
      721 cttcgcccgc ttccagcctc aaaccgtaag taagtgccaa atcacccgaa cgtgctgccg
      781 gcgaaacgac aaactcaccg aagaaatcaa aatgctcacg gacgctaata cccgtctcgg
      841 taaagtattt cgccatttta tagtgaacta aatt
//
LOCUS       DAWHRA010000251          859 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWHRA010000251 DAWHRA010000000
VERSION     DAWHRA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.251"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(38..604)
                     /locus_tag="WIB30_002108"
     CDS             complement(38..604)
                     /locus_tag="WIB30_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ3798102.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWVAFVLLGGLGLKMMREGLSGEAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTTGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          246 a          281 c          170 g          162 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactatattt tccgacatca ttgaatcaaa cccaaatgcg
       61 acaagagcgt ccatgtgccg atggcaatca acaccaaacc tccggcaaat tccgcacacc
      121 tgccgaacaa tacgcccaaa gcccttcccg tcgtcagccc gaccgccacc atcaccgtcg
      181 tcgccatacc gatgattgcg gcggcaaagg cgatgtttac ctccataaac gccaagccca
      241 ccccgactat catggaatca atactggttc caaaagcagt caaaaccgtc atccataggc
      301 tttcccgttt gctttcgcgc acatcttccg cctcgccgga cagcccttcg cgcatcattt
      361 tcagacccag cccgcccagc aggacgaaag ccacccaatg gtcccattcg ctgataaacg
      421 gcttggcata aaaaccgcct acccagcctg ccagcggcgt gagcgcttca accgtgccga
      481 acaccaaagc cgttgccgca attttgcgcg gaggcattct gaccgccgca ccctttgcca
      541 atgcgacggc aaacgcatcc atcgacatcc ccagagcaat caagagcaaa gcataaaaac
      601 ccataccgca cccgtcctca aaaagggcgg attatagcaa aagcaaaaaa atgcaaaaat
      661 gccgcacgaa aacccgcatc ccgtcattcc cgcaaaaaca aaaaatcaaa aacagaaatc
      721 ccgtcattcc cgcgcaggcg ggaatccaga gttgtcggtg cggaaactta tcggataaaa
      781 cggtttctcc aaccccgagt ccttgattcc cactttcgtg ggaatgacgg gatattttgc
      841 gtttaataaa aaacgccca
//
LOCUS       DAWHRA010000252          843 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWHRA010000252 DAWHRA010000000
VERSION     DAWHRA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.252"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..79)
                     /locus_tag="WIB30_002109"
     CDS             complement(<1..79)
                     /locus_tag="WIB30_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3798103.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTA"
     gene            677..843
                     /locus_tag="WIB30_002110"
                     /pseudo
     CDS             677..843
                     /locus_tag="WIB30_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Caudovirus prohead protease"
BASE COUNT          221 a          222 c          153 g          247 t
ORIGIN      
        1 gggcggtaac ttcgagtaca aaaaaacgga gcagttcttt ctgtactttt ttctttaatt
       61 tgcagtgcgt tatcttcata tttcgagggt aacatatctg ctaatctagt acagccccta
      121 gtttaaagct ttgtttctta agtccgcaga gtatgccatg gttagacctt caaagttgag
      181 tattgtacta ttttgttttt aattaactat aaaacgcttg tgtgaatagg aagactgcag
      241 aatattgctg ccttttcctg tttgctttaa ataaacagaa aatgccgtct gaaaccgatt
      301 ttcagacggc atttgtccct taaaacacgt tttttcaagc gcgaccatac caaacaccca
      361 caggttgcct tccacccttc gtacaacctt tgctccatca aacatccact tcctaaatac
      421 tgatgtctgc ttaggttgaa tcggcattcg tacgattacg gcacaccgcc taatctgtac
      481 ctgcgtctgt tgcgcccaat accaaaggct acctgaaaat ctcccaaact tttcaggtag
      541 ccttatcacg ttttcgactg tcaccacacg cccaaagacc gcctgaaacc ctttttcaga
      601 cgttccgctc cgataccccc aacaaaacgc cgcccgaacg catgttcaga cggcattttc
      661 tttgcgtcga ttcattgcgg tgtctacctg ctttaaggcg gacaaaggtg cggaagcggt
      721 aaacaacgat acagaacata atgctacata ttttagccat ttcccttcca aacaaaaaag
      781 caccgacggc ggccgatgcc ctttccttta caggttcccc tattttttat ccgcgggcag
      841 cac
//
LOCUS       DAWHRA010000253          838 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWHRA010000253 DAWHRA010000000
VERSION     DAWHRA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.253"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..798
                     /gene="ttcA"
                     /locus_tag="WIB30_002111"
     CDS             <1..798
                     /gene="ttcA"
                     /locus_tag="WIB30_002111"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ3798104.1"
                     /translation="LRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKI
                     VEEDTYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFL
                     NMFYGGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPN
                     LQRQVIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGD
                     LAFDSEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGS"
BASE COUNT          227 a          262 c          192 g          157 t
ORIGIN      
        1 ctgcgccagc tccaagccag cgcgccgatt gatttccaac tggttgccgt caatctcgac
       61 caaaagcagc cgggcttccc cgaagaagta ttgccgacct atctcgaaag catcggcgtt
      121 ccctacaaaa tcgttgaaga agacacctac tccaccgtca aacgcgtatt agacgaaggc
      181 aaaacgactt gttcgctgtg cagccgcctg cgccgcggca tcctctaccg cactgcaaaa
      241 gaattgggct gcaccaaaat cgccttggga caccaccgcg acgacatcct cgccaccttg
      301 tttttaaata tgttttacgg cggcaaactc aaagccatgc cgcccaagct ggtgagcgac
      361 aacggcgaac acatcgtcat ccgcccgctg gcgtatgtga aagaaaaaga cttaatcaaa
      421 tacgccgaat taaaacaatt ccccatcatc ccgtgcaacc tctgcggctc gcaacccaac
      481 ctgcaacgcc aagtcatcgg cgatatgctg cgcgattggg acaaacgctt ccccggccgc
      541 atcgaatcga tgttctccgc cctgcaaaac gtcgtccctt cccaccttgc cgataccgaa
      601 ctcttcgact ttgtcggatt ggaacgcggt caaagcctga aacacggcgg cgatttggcg
      661 tttgacagcg aaaaaatgcc cgaacgtttt tcagacggca gcgaagaaga cgagagcgaa
      721 atcaaaatcg agccgcaaaa agccgaacgc aaagtcatca atattctggc gaacaagccg
      781 aaaacctgcg gctcatagac ctccgccgtc aaaaatgccg tctgaagccc gtttggcg
//
LOCUS       DAWHRA010000254          790 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWHRA010000254 DAWHRA010000000
VERSION     DAWHRA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.254"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          199 a          204 c          192 g          195 t
ORIGIN      
        1 tcgttcccac ggtcaggatt tcagattgcg gacatctgtc agaaaagaca aaaaaccttc
       61 cgccgtcatt ccctacaggc gagaatccga tccgttgaaa ttcggttgtt ttaaataaat
      121 tcttgcagct ttgattttct gtttttccga taacgccgta actttgaaac gcgaaagcgg
      181 taatccgatc cgttgggatt ttgcaacttc aaatcaatcc gcaaactgaa atcccgtcat
      241 tcccgcgcag tcgtgaatcc gaacgcgtcc gcacgaaaac ctgcatcccg tcattcccgc
      301 gcagtcgtga atccgaacgc gtccgcacga aaacctgcat cccgtcattc ccacggaagt
      361 gggaatctag gacgtaaaat ctaaagaaac cgttttatcc gataagtttc cgcaccgaca
      421 gacctggatt cccgcctgcg cgggaatgac gaaatttcgg cgagccgtag ggtgggctgt
      481 agggtgggct tcagcccacc aatttcaccg catcaaaggt ttttgggaat acggcggttt
      541 cggttttcgg tgggaatggg cggaatggat aacattgacg gagttggggg aataggtgga
      601 aacggtggga ttggtgggct gaagcccacc ctacagccag ccctatgtcc acgctgcaca
      661 tcctacgccc gccttgcgtt tcttataagc agcattttac gcaatacttt gcgtccgctg
      721 aaaaaatttc aaacggtctt ttttatcaaa tgcgaaaaat ataacgtcat tcccgcgcag
      781 gcgggaatct
//
LOCUS       DAWHRA010000255          789 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWHRA010000255 DAWHRA010000000
VERSION     DAWHRA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.255"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            5..237
                     /locus_tag="WIB30_002112"
                     /pseudo
     CDS             5..237
                     /locus_tag="WIB30_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            296..604
                     /locus_tag="WIB30_002113"
     CDS             296..604
                     /locus_tag="WIB30_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798105.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMGASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTIYKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK
                     "
     gene            complement(586..789)
                     /locus_tag="WIB30_002114"
                     /pseudo
     CDS             complement(586..789)
                     /locus_tag="WIB30_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          241 a          192 c          176 g          180 t
ORIGIN      
        1 gaaaatgttg aaaacaaaaa gaactcattg cttgaaaaaa gatttaatgg gaatgcgggt
       61 atttcccaca ctttttccat ttatttatcc tcattatagg aagaaaaaca tgaaaaaacc
      121 ctgcttgcac tctcacttgc agccgtgtcc gccgttacgg tagccgatat tcccgagtct
      181 aacggcaaag gcaaagccat catcggcgat aaggaaaaac accgttacca agcatgaaaa
      241 ccttgctttg aaagtccatg gcgaaatccg ccaagatttg ggggacggat gatccctgac
      301 cccttgggcg gatgcatatg cagatttgcg cggcaaaacc aaagtgatga cgacccagat
      361 gggtgcttcc cgcgatgtca gcaaaagcgc caaaggttgg agtgtcggta tcggtctgaa
      421 tgtaggcaaa cagttgaccg acagcgtcgg tctcgagttt gatccatact accgtcacaa
      481 aacaatctac aaaccccgtg agattgtctt ggacggtgac aaaaccaaaa tgggccgctc
      541 caaatccaac gagtacggct tccgcgtagc cgcaacgttc tatagtcaat taaaatcaaa
      601 ataggacagt agtgcatcgt caaatcgggc gtaatcagac aaaacggttc gcagataccg
      661 cttaatattc gcccacactt tctcaatcgg gttgagctca ggtgaataag gtgcaagagg
      721 caatacctta tgtcccaatt tttccgccat ttcccgtaag acacccatac ggtgaaatca
      781 cgcattatc
//
LOCUS       DAWHRA010000256          781 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWHRA010000256 DAWHRA010000000
VERSION     DAWHRA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.256"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..323
                     /locus_tag="WIB30_002115"
     CDS             <1..323
                     /locus_tag="WIB30_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ3798106.1"
                     /translation="PYIGARVAYGHVRHSIDSTKKTTEVVTSTHGGADTKPTIYNGES
                     TQNAYHESHSIRRLGLGVVAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGM
                     RYRF"
BASE COUNT          199 a          241 c          178 g          163 t
ORIGIN      
        1 aaccctatat cggtgcgcgc gtcgcctacg gacacgtcag acacagcatc gattcgacca
       61 aaaaaacaac agaggttgtt acctccaccc atggtggtgc tgacacaaaa cctacgattt
      121 ataatgggga aagtacgcaa aacgcctatc acgaaagcca cagcatccgc cgcttgggtc
      181 ttggtgtcgt cgccggtgtc ggtttcgaca tcacgcccaa gctgacttta gacaccggat
      241 accgctacca caactgggga cgcttggaaa acacccgctt caaaacccac gaagtctcat
      301 tgggcatgcg ctaccgcttc tgattccccg ataccgatgc cgtctgaacc ttcagacggc
      361 attttttaca caattcccac cgtttcccat cattcccgat acaccgtaat ctcgaaaccc
      421 gtcattcccg cgcaggcggg aatccagacc tgtccgcaca gaaacttatc ggataaaaac
      481 agttgcccaa accccgcgtt ctagattccc actttcgtgg gaatgacgtg gtgcaggttc
      541 cgtatggatg gattcgtcat tcccgcgcag gcgggaatcc aaacccccga cgcggcggga
      601 atctatcgga aatgactgaa accccgagat tctagattcc cactttcgtg ggaatgacgg
      661 ttcagttgcg ttccgacaac accgtaatct tgaaatccgt ccgacaacac cgtaatctta
      721 aaacccgtca ttcccgcgca ggcgggaatc cagacctccg acgcggcggg aatctatcgg
      781 a
//
LOCUS       DAWHRA010000257          755 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWHRA010000257 DAWHRA010000000
VERSION     DAWHRA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.257"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            328..>755
                     /locus_tag="WIB30_002116"
     CDS             328..>755
                     /locus_tag="WIB30_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ3798107.1"
                     /translation="MQPPKNLLFSSLLFSSLLFSSLLFSSLLFSSAAQAASEDSGHGP
                     YYVQADLAYAAERITHDYPKATGADKDKISTVSDYFRNIRAHSIHPRVSVGYDFGGWR
                     IAADYASYRKWNNNKYSVNTKKVQENQGTRKDLKTENQENG"
BASE COUNT          210 a          196 c          154 g          195 t
ORIGIN      
        1 agtccgttcg gtttcggtcg tttccgataa attcctgctg cttttcattt ctagattccc
       61 actttcgcgg gaatgacggc tgcagatgcc cgacggtctt tatagtggat taacaaaaat
      121 caggacaagg cgacgaagcc gcagacagta cagatagtac ggaaccgatt cactcggtgc
      181 ttcagtatct tagagaatcg ttctcttttt tgttcatccg ctatattgtg ttgaaacatc
      241 gccccaaacc cgatataatc cgcccttgaa ccatcagtga aaatcgttct ttttaatcag
      301 ttaaaccgaa tacggagtcg aaaatgaatc cagcccccaa aaaaccttct cttctcttct
      361 cttctcttct cttctcttct cttctcttct cttctcttct cttctcttct cttctcttcc
      421 gcagcgcagg cggcaagtga agacagcggg cacggcccgt attatgtgca ggcggattta
      481 gcttatgccg ccgaacgtat tacccacgat tatccgaaag caaccggtgc agacaaagac
      541 aaaataagca cagtaagcga ttatttcaga aacatccgtg cgcattccat ccaccctcgt
      601 gtgtcggtcg gctacgactt cggcggctgg agaatagcgg cagattatgc cagttacaga
      661 aaatggaaca acaataaata ttccgtcaac acaaaaaagg tgcaagaaaa ccaaggcacc
      721 agaaaagacc tgaagacgga aaatcaggaa aacgg
//
LOCUS       DAWHRA010000258          748 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWHRA010000258 DAWHRA010000000
VERSION     DAWHRA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.258"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..427
                     /locus_tag="WIB30_002117"
     CDS             <1..427
                     /locus_tag="WIB30_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ3798108.1"
                     /translation="KQIDDHTHTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSH
                     KRIASLAGIAPHPRESGETKFKSRCFGGKSAVRKALYMATVAATRFKPLIRDFYQRLL
                     SEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENGI"
BASE COUNT          177 a          174 c          211 g          186 t
ORIGIN      
        1 caaacaaatc gacgaccaca cccacacgca ttttgacggc aaagcccaag tggcggagca
       61 aatcaaaggc atcggttcga taacgacggc tacgctgatg gcgatgctgc ccgaattggg
      121 gcggctgtcg cacaaacgga tagcgagttt ggccggcatt gccccgcacc cgagggagag
      181 cggggaaacc aaattcaaaa gccgctgctt tggcggaaag tctgcggtac gtaaggcgct
      241 gtatatggct accgtggcag cgacacgttt taaaccgctt attcgggatt tctaccaacg
      301 cctgctgtcc gagggtaagc cgtataaggt tgccgttacg gcatgtatgc gcaaactgct
      361 gacgatatcg aatgcccgga tgcgtgatta ttttgccgaa aacgataccg ccgaaaacgg
      421 tatctaaacg gcttgatttg agttttggta tttttgcccg acggggtgaa aaatacagtt
      481 gctacggctc gatgaatcgt cagaaatacc tgcatcgtca ttcccgcgca ggtgggaatc
      541 cagaccggtc ggtgcggaaa cttatcaggt aaaacggttt cttgagattt ttcgtcttgg
      601 attcccactt tcgtgtgaat gacggaatgt aggttcgtgg gaatgacgtg gtgcaggttt
      661 ccgtatggat ggattcgtca ttcccgcgca ggcgggaatc tagtctgttc ggtttcagtt
      721 attttcgata aatgcctgtt gcttttca
//
LOCUS       DAWHRA010000259          723 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWHRA010000259 DAWHRA010000000
VERSION     DAWHRA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.259"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          201 a          151 c          174 g          197 t
ORIGIN      
        1 ttcgtaaaag gcgggctata gggtaggctt catctcgcca atctcactga atccatcaat
       61 ttccacaatt caattaaata ccgtcaaacc gatgccgtca ttcccgcgca ggcgggaatc
      121 tagaccttgg gataacagca atattcaaag tttataaaag acccgtcatt cccgctcagg
      181 cgggaatcta gaccttagaa caacagtaat attcaaagat tatctgaaag tccgggattt
      241 tggattccca ctttcgtggg aatgacggaa tgcaggtttc cgtatggatg gattcgtcat
      301 tcccgcgcag gcgggaatct agaccttaga acaacagtaa tattcaaaga ttatctgaaa
      361 gtccgggatt ctggattccc actttcgtgg gaatgacgtg gtgcaggttc gtaggaatga
      421 cgtggtgcag gtttccgtgc ggatggattc gtcattcccg cgcaggcggg aatccagacc
      481 ttagaacaac agcaatattc aaagattatc tgaaagtccg ggattctgga ttcccacttt
      541 cgtgggaatg acgggatttt aggtttctga ttttggtttt ctgtttttgt aggaatgatg
      601 aaattttgag ttttaggaat ttaccggaaa aaacagaaac cgctctgccg tcattcccgc
      661 gcaggcggga atctagacct tagaacaaca gtaatattca aagattatct gaaagtccgg
      721 gat
//
LOCUS       DAWHRA010000260          718 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWHRA010000260 DAWHRA010000000
VERSION     DAWHRA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.260"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>718
                     /locus_tag="WIB30_002118"
     CDS             <1..>718
                     /locus_tag="WIB30_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798109.1"
                     /translation="TRLEKFDRVALQHFSQYVDLINENNGRLPNTSEIERSYYKAVTE
                     NGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNAQAVNNPNGSEWDNRKQLIS
                     ALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIGGWGNGIINDLYKSVAKR
                     EWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKETKKRNGKYHVYDPLALDL
                     DGDGIETVATKGFAGSLFDHT"
BASE COUNT          239 a          110 c          163 g          206 t
ORIGIN      
        1 acccgtttag agaaatttga tcgggttgca ttgcaacatt tcagccaata tgtagatcta
       61 attaatgaaa ataatggtag attacctaac actagtgaaa ttgagagaag ttactataaa
      121 gccgttaccg aaaatggtgt ttcttctagt gcagctattg atttagttat taatcgctca
      181 cttccggata tggcagatgg ttattgggca ttaggtttgg ggatagaagc cgaacgtatc
      241 cacaatgcgc aagcagtaaa taatccgaac ggtagcgaat gggataatag aaagcagtta
      301 atatctgctt tagataaagg atttgatgga tcttttaaag agaagcattt tactttttta
      361 caatctgtga taatggatgt aacaaagtta ggtgttgaat atacaataga tggttggcaa
      421 aaaattggag gttggggtaa tgggataatc aatgatttat ataaaagtgt tgcaaaaaga
      481 gagtggactg gaatatttga gatcgttaat aataacatca agcaatttag agatctgttc
      541 ccaaatccgg aaggctggat cgatgatggt caccaatgtt tcgctccttg ggttaaagaa
      601 actaaaaaac gcaatggcaa atatcatgtc tacgaccccc tcgccctaga tttggacgga
      661 gacggtatag aaaccgttgc caccaaaggc tttgcaggca gcttatttga tcacacca
//
LOCUS       DAWHRA010000261          682 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWHRA010000261 DAWHRA010000000
VERSION     DAWHRA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.261"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(80..565)
                     /gene="fxsA"
                     /locus_tag="WIB30_002119"
     CDS             complement(80..565)
                     /gene="fxsA"
                     /locus_tag="WIB30_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ3798110.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          200 a          217 c          121 g          144 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatatttt caaaccggaa aaatgccgtc tgaatcttca
       61 gacggcattc cattcatatt tattcgtctt ttttgtgttc gatggcgttt cgggaacgat
      121 tgccgccgta aggctcttca accgtatatt ccccctcgat aatatcgtca tcgcgggaaa
      181 agccctcttt tctgcccgat tggttcatgt tgaaaaaatt ttccgcacct cctgcctgca
      241 acactgcacc tcccttaaac ggcagcagca gcaacaccgc caacaccgag gatacgaatc
      301 ccggactcat cagacacacc gccgccaccg tataacggat aggccacaac atctgataaa
      361 cggataccct cccgccgctt ctcattgccg cgcccgccaa taaaagaccg gacagccccg
      421 tatgcctgag catcagcaca ccggcggcaa aacctgccgc catcaaaaac aacgtccagc
      481 cgccgcccaa ccaatcggca acccacacaa tcgacataat ctccaaaaac agcagcacca
      541 aaaaaccgat accgaaaaat ctcattgacc gtcatcctta tatttaagta aacagcaaac
      601 cgcccgaaca ggactccaag cgacctgcct gtaaatgatt acaaaaccat gtgcttcaag
      661 ccgaaacaat gtgaaatcac gc
//
LOCUS       DAWHRA010000262          663 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWHRA010000262 DAWHRA010000000
VERSION     DAWHRA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.262"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(136..629)
                     /locus_tag="WIB30_002120"
                     /pseudo
     CDS             complement(136..629)
                     /locus_tag="WIB30_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
BASE COUNT          153 a          156 c          146 g          208 t
ORIGIN      
        1 cgagttcgtc ggcaacggcc gctgcggctt gggcggcgtt ttgccgaacg cctgcgttac
       61 ttccctttgt aggtcgatct ctttggcaac cgcgtctttg tcaaagctgt ttttcagacg
      121 gccttttgtc aaatattatc ggcagtggct caatgccaac tttaaacctg ctccgatttc
      181 ttcagggctg ttatccaatg ataaaatgac atcgtctgca tcaatggcat tccacgcttc
      241 cagcttgaca tggcggctcg ggctgatttt caggcagccg ttgtgcagcc aaatatccac
      301 gctcatcatg tttttaaata gggcgcgtct ggttttatag cccaagttcc cgcatagctt
      361 ggcaacccaa tcctcatagc gttgccgaat tttttcggta tcaaaaaaat cttggtcttc
      421 tggactgtca taaacgaaag tcctgctgtt tgccaacgct tgcaagactg tcgtgcctaa
      481 agtttcattg tcggtatcca atggcaggat atggggggat ataggtggtc tattgccgtt
      541 aaccccaaac ctgaacatgt ttgaacaatc agagtttctt tattagcatc aaaaactgcc
      601 caataatttt gattctgttt aaaaatcatt atttgatctc cgtaattttg actgtatagt
      661 gga
//
LOCUS       DAWHRA010000263          599 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWHRA010000263 DAWHRA010000000
VERSION     DAWHRA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.263"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..207)
                     /locus_tag="WIB30_002121"
     CDS             complement(<1..207)
                     /locus_tag="WIB30_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEZ3798111.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHP"
     gene            complement(271..444)
                     /locus_tag="WIB30_002122"
     CDS             complement(271..444)
                     /locus_tag="WIB30_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ3798112.1"
                     /translation="MKSERRHWEWCVDYDEEGVVRDYRFTHKVGKDERSVIRDTVDVI
                     RKEAGKSLSQPEK"
BASE COUNT          130 a          163 c          149 g          157 t
ORIGIN      
        1 ggggtgtgcg tgtccgacaa tcgcaggacc ccaagagccg acgtaatcgg tgtagcgcgt
       61 gccgttttcg tcccaaacat atgcgccttc ggcttttttg ataaagcgcg gcacaccgcc
      121 gacgctgccg aatgcgcgga cgggggaatt cacgccgccg gggatgatgg ctttggcgcg
      181 gtcgaataaa atttcgttac ggttcatatt tatcctcaaa tgccgtctga acggcaggtt
      241 tcgggcttgg aagcagaaag ccccatttta tcatttttca ggttgcgaca aggatttgcc
      301 cgcttctttg cggatgacgt caaccgtatc ccggatgacg gaacgctcgt cttttcccac
      361 tttatgtgta aagcggtagt cccggacaac tccctcctcg tcgtaatcca cacaccactc
      421 ccaatgtcgg cgttctgatt ttatataaat gaaattggtc ggtaaaaaat tataaatagg
      481 caggctgact tcatgatagg cataacaacc gaacggattt tgccgcccga aacgtgcctc
      541 tacctccgcc cgggtcgttt tgcctttaac aaccgtttgt gcgattccct cttccgtct
//
LOCUS       DAWHRA010000264          594 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWHRA010000264 DAWHRA010000000
VERSION     DAWHRA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.264"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(249..>594)
                     /locus_tag="WIB30_002123"
     CDS             complement(249..>594)
                     /locus_tag="WIB30_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ3798113.1"
                     /translation="DMIRYPLLSAGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGI
                     DYPPALITTSLSDDRVHPAHALKFYAKLRETSAQSWLYSPDGGGHTGNGTQRESADEL
                     ACVLLFLKEFLG"
BASE COUNT          122 a          143 c          187 g          142 t
ORIGIN      
        1 gcgatttcgg tgacggtgca gtggcgggac aggtattgga tgtggtatcg ttcgccttgg
       61 gtcagttgcg tgtagctcat ggcaatcttt cttgcaggaa aggccgtatg ctaccgcata
      121 ctggcctttt tctgttaggg aaagttgcac ttcaaatgcg aatccgccaa ccattaaaaa
      181 aggtcgtctg aaaaagcagt ttcagacgac cttttttcat tcgcggcggc agtgccgctc
      241 ccccgccctt agcccaaaaa ctctttcaaa aacagcaaga cgcaggcgag ttcgtcggcg
      301 gattcgcgtt gggtgccgtt gccggtatgg ccgccgccgt caggcgagta gagccaagat
      361 tgcgcggagg tttcgcgcag tttggcgtag aacttgagcg cgtgggcggg atggacgcga
      421 tcgtcggaca ggctggtggt aatgagcgcg ggcggataat cgatgccgtc tgaaagattg
      481 tgatacggcg acaattcgcc caaccggcgt ttgcagactt cgtatttttg cggattgccg
      541 tattcgtctg tccagcttga accggcggag agcagcggat aacggatcat gtcg
//
LOCUS       DAWHRA010000265          579 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWHRA010000265 DAWHRA010000000
VERSION     DAWHRA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.265"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..116)
                     /locus_tag="WIB30_002124"
     CDS             complement(<1..116)
                     /locus_tag="WIB30_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ3798114.1"
                     /translation="MRKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREET"
     gene            175..396
                     /locus_tag="WIB30_002125"
     CDS             175..396
                     /locus_tag="WIB30_002125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798115.1"
                     /translation="MLSTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQAEI"
BASE COUNT          142 a          174 c          130 g          133 t
ORIGIN      
        1 gtttcctcac gttgcgcgcc ggcgtgcgcc agaggactga gcaacaacgc accggcggca
       61 cacaacaggc ggcggcgttg cagattaacg ggtagggtat cggtcggttt tctcataggg
      121 aacgggggcg cgtccggacg tttcagacgg cattaaatat tcaaacagac ataattgctt
      181 tcaacgcgaa aaaccgcgcg caaaatccaa gcgcggcata tcgccctgcc cttttcgggc
      241 aaacctcaat tctaccgccc tcaagaacgc ttgtccaaac aggcacaggc aacaccgccc
      301 gggcatttcc gttttcaccg gttatccgtc gtccggatta tgcagcagca ccatcagcgc
      361 atcacgcttt tcgggcggca gcaggcggaa atatagtaga ttaaatttaa accagtacag
      421 cgttgcctcg ccttgccgta ctgatttaaa tttaatccac tatatcttga ggcctttgca
      481 aaattccttt ccctcccgac agccgaaacc caaacacagg ttttcggctg ttttcgcccc
      541 agatacctcc taattttacc caaatacccc tttaatcct
//
LOCUS       DAWHRA010000266          555 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWHRA010000266 DAWHRA010000000
VERSION     DAWHRA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.266"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            425..>555
                     /gene="pilE"
                     /locus_tag="WIB30_002126"
     CDS             425..>555
                     /gene="pilE"
                     /locus_tag="WIB30_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HEZ3798116.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEA"
BASE COUNT          202 a          149 c           77 g          127 t
ORIGIN      
        1 cagaaaacgg aaatttttaa aaattaaatt taaaatttcc cacccaaccc accctatgct
       61 accgcgcaaa ttcaaaaatc tcaaattccg acccaatcaa cacacccgat gccccatgcc
      121 aatagagata ccccacacca ataaaaaagt gccgaaaatc ggcactaaaa ctgacaattt
      181 tcggcattac tgacaaaaaa cggtaacccc ccgcttcctc aaaccacacc caaacgaaag
      241 aaaatgtaaa ataaaaacaa ccacataaag ataaacgcat aaattttcac ctcaaaacac
      301 aaacttgata ccaatcttgc tttataatac acaggtatcg caacaaaata ccgatggtca
      361 aatacattgc ataatgccga tggcgtaagc ttgaggcatt tcctttccaa ttaggagtaa
      421 ttttatgaac acccttcaaa aaggttttac ccttatcgag ctgatgattg tgattgccat
      481 cgtcggcatt ttggcggcag tcgcccttcc tgcttatcaa gactacacag cccgcgcaca
      541 agtttccgaa gccat
//
LOCUS       DAWHRA010000267          553 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWHRA010000267 DAWHRA010000000
VERSION     DAWHRA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.267"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>553
                     /locus_tag="WIB30_002127"
     CDS             <1..>553
                     /locus_tag="WIB30_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ3798117.1"
                     /translation="SGSKGWVVKLKDGQRVTVKPTVVLRTAFVTIRKYKDNGCGAETA
                     ILGINTADGGKLTKKSARPIVPADNTAVAQYSGHKQTTKGKSIPIGCMQKGNEIVCPN
                     GYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPDGKRSGKNNRCFSQKGVRTLLM
                     NDLDSLDITGPTCGMKRISWREVF"
BASE COUNT          156 a          144 c          154 g           99 t
ORIGIN      
        1 tcgggcagca aagggtgggt agtgaaattg aaggacggac agcgcgttac cgtcaaaccg
       61 accgtggtat tgcgtaccgc ctttgtaacc atccgcaaat ataaagacaa cggctgcggc
      121 gcggaaaccg ccattttggg catcaatacc gccgacggcg gcaagctgac gaagaaaagc
      181 gcgcgcccga ttgtgccggc cgacaatacg gctgtcgcgc aatattccgg ccataagcaa
      241 accaccaaag gcaaatccat ccctataggt tgtatgcaaa aaggcaatga aatcgtctgc
      301 ccgaacggat atgtttacga caaaccggtt aatgtgcgtt atctggatga aaagaaaaca
      361 gacggatttt caacaacggc agacggcgat gcgggcggca gcggtataga ccccgacggc
      421 aagcgttccg gcaaaaacaa ccgctgcttc tcccaaaaag gggtgcgcac cctgctgatg
      481 aacgatttgg acagcttgga cattaccggc ccgacgtgcg gtatgaaacg aatcagctgg
      541 cgtgaagtct tct
//
LOCUS       DAWHRA010000268          552 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWHRA010000268 DAWHRA010000000
VERSION     DAWHRA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.268"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            161..490
                     /locus_tag="WIB30_002128"
     CDS             161..490
                     /locus_tag="WIB30_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3798118.1"
                     /translation="MGSLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGARGAGGVIPPHATLIFEV
                     ELLKVYE"
BASE COUNT          165 a          140 c          146 g          101 t
ORIGIN      
        1 tccatgaaaa agcatggaac ctttacgaaa aaaataaaac agtccaacaa acacagccgt
       61 ttcaactgca ccggtgcggc tgacgtttga gcggcatttg acttcgccgg acggtatgtt
      121 acccttgccg ttttgaccaa caagcaacga aaggacaaat atgggcagcc tgattattga
      181 agatttgcag gaaggcttcg gaaaagaagc agttaaaggc aaagagatta ccgtgcatta
      241 cacaggttgg ctggaaaacg gaaccaaatt cgactccagc ctcgaccgcc gccagccgct
      301 gaccatcacg ctcggcgtcg gacaagtcat caaaggctgg gacgaaggct tcggcggcat
      361 gaaggaaggc ggcaaacgca agctgaccat cccttcggaa atgggctacg gcgcacgcgg
      421 cgcgggcggc gtgattcctc cgcacgccac tttgatattt gaagtcgagc tgctgaaagt
      481 gtacgaataa agctgcccgg gcaataccgt ctgacataat ggcttcaagc aacgtaaaca
      541 acgaaatcaa ag
//
LOCUS       DAWHRA010000269          550 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWHRA010000269 DAWHRA010000000
VERSION     DAWHRA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.269"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            428..>550
                     /locus_tag="WIB30_002129"
     CDS             428..>550
                     /locus_tag="WIB30_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798119.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLHLSLGY"
BASE COUNT          154 a          113 c          157 g          126 t
ORIGIN      
        1 cttgccgcct gcgctgcgga agagaagaga agagaagaga agagaagaga agagaagaga
       61 agagaagaga agagaaggtt ttttggggct ggattcattt tcgactccgt attcggtttt
      121 aactgattaa aaagaacgat tttcaatgat gttgcaggag cggactatat caggtttggg
      181 gcgatgtttc aacacaatat agcggatgaa caaaaaagag aacgatgctc taaggtgctg
      241 aagcaccgag tgaatcggtt ccgtactatc tgtactgtct gaggcttcgt cgccttgtcc
      301 tgatttttgt taatccgcta taaacaacgc ttcgtccaaa aaaacgattg aatttgcggg
      361 cagaagctgg acgaaaaccg ccgacagcct gccgcaaaag gcacacggtt tgcgctaggg
      421 cttaggcgtg tcgcgcgaaa tcaatgcggg cgggcatcat ttcctctacg gcggcatcag
      481 cggcggcggc gtgcattatt gggataacaa agatttcagc gaacagtgcc tgcacctgtc
      541 gctcggctat
//
LOCUS       DAWHRA010000270          550 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWHRA010000270 DAWHRA010000000
VERSION     DAWHRA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.270"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            291..470
                     /locus_tag="WIB30_002130"
     CDS             291..470
                     /locus_tag="WIB30_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798120.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNPDTDEKRQ"
BASE COUNT          134 a           99 c          153 g          164 t
ORIGIN      
        1 ttcaggtatt gttcgatcgg ctgccaatca atcacctggt ccaacttcaa tagtgggaaa
       61 cggtcgatgt gtttggcaat catggcttgg gcggtttgct ggaagaaggt gctcctaaat
      121 gtcttggtgg gaatttaggg gattttgggg aattttgcaa aggtctctat ttgtactgtc
      181 ttcggctcgc cgccttgtcc tgatttaaat ttaattcact atatttgccc gtgcgtttgt
      241 gataaattcg gcactgtatt tgatatgatt ttaacaaatc gggaggggca atgttttggt
      301 atgtgattgg cttttgcgcc tttgtcgtcg cgctgctgtc gctgtgggtc aatgccggcg
      361 cgttcggtat gcaggaagac gatactccgc aatcggatta tgaaaggcgt ttgggcttgg
      421 gtgcgaagct gaaaaacaaa aatccggata cggacgaaaa acggcaataa agacaacaaa
      481 tgccgtctga aaggttttca gacggcattt gctttggagc ggatatagtg gattaacaaa
      541 aatcaggaca
//
LOCUS       DAWHRA010000271          548 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWHRA010000271 DAWHRA010000000
VERSION     DAWHRA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.271"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            1..>548
                     /locus_tag="WIB30_002131"
     CDS             1..>548
                     /locus_tag="WIB30_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003796842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3798121.1"
                     /translation="MPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQAAELIPNQ
                     AMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRTALFAGYE
                     SLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGEETIRGKL
                     ANWERFIHESGDLDPLIIMAAA"
BASE COUNT          164 a          162 c          122 g          100 t
ORIGIN      
        1 attccctata acgatttacc acccctgccg ccaaaacagg atattgaaag caaaaccatc
       61 ctgaaacgtt gtatagccgc ccgtgcatcc cttgcccgtt taaagcaggc ggcagaattg
      121 ataccgaatc aagccatgct gattaacacc cttcctgtta tggaagcccg tgcaagttcg
      181 gaaattgaaa acatcgtaac cacaacggac aagctgtttc aatccctgca aatggatacg
      241 gaacggcaag accctgccac gaaagaagcc ctgcaatacc gcaccgccct gtttgcaggc
      301 tatgaatcac tgacgagccg ccctttatgc acacaaaccg ccatcatggt ctgcaacgcc
      361 atcaagcacc cctacgaaat ggccatccgc aaaacaggcg gcacagccct aaaaggaggc
      421 aacagcggaa atgttgtcta taccccgccc gaaggagaag aaaccatacg cggcaagctg
      481 gcaaattggg agcggtttat tcacgaaagc ggcgatttag acccgcttat catcatggcg
      541 gcggcaca
//
LOCUS       DAWHRA010000272          547 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWHRA010000272 DAWHRA010000000
VERSION     DAWHRA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.272"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            363..>547
                     /locus_tag="WIB30_002132"
     CDS             363..>547
                     /locus_tag="WIB30_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ3798122.1"
                     /translation="MSTFFQQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMF"
BASE COUNT          164 a          144 c          104 g          135 t
ORIGIN      
        1 cgcccatacc cgtatcaata tcgttgaact tccatttttt accctgcagc ggatgtgcgg
       61 ttaaatattt tggcggaaaa gcaaagaagt atacttcttt ttagttatgg ttgattccat
      121 aactaaaaaa gtatcgataa acccattttg cgaggtacgg ataatgtctg acagctttga
      181 aaacagcccc gaatacgata attggatcga atcgggcggt cgtgatgaag attacgaata
      241 ctactacaac aaatggcaac gcaggacgcg ctgaccatta aaccctgaca aaccggtttg
      301 agacctttgc aaaattcccc aaaatcccct aaattcccac caagacattt aggggatttc
      361 tcatgagcac cttcttccaa caaaccgccc aagccatgat tgccaaacac atcaaccgct
      421 tcccgctatt gaagttggac caggtgattg attggcagcc gatcgaacaa tacctgaatc
      481 gtcaaaaaac ccgttacctc agagaccacc gcggccgtcc cgcctatccc ctgttgtcca
      541 tgttcaa
//
LOCUS       DAWHRA010000273          519 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWHRA010000273 DAWHRA010000000
VERSION     DAWHRA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.273"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..272
                     /locus_tag="WIB30_002133"
     CDS             <1..272
                     /locus_tag="WIB30_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798123.1"
                     /translation="MEKDKAQAMLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKK
                     QAEVSRLKTERDLCKIPFPPDSRNPNTGFRLFSPQIPPNFTQIPP"
     gene            complement(314..>519)
                     /locus_tag="WIB30_002134"
     CDS             complement(314..>519)
                     /locus_tag="WIB30_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3798124.1"
                     /translation="ETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQ
                     SHLKAMCLNLLKAANRLSVPAAA"
BASE COUNT          134 a          145 c          125 g          115 t
ORIGIN      
        1 agatggaaaa agacaaagcg caagccatgc tgttgtcggc tcaaaactat gcacgcgagc
       61 tggaacaggc gcgtgcggaa gcgaaaaaat atgaagtcaa ggcgcacgcc gtcggcatgg
      121 ctttggcgaa aaaacaggcg gaagtcagcc gtctgaaaac ggaaagagac ctttgcaaaa
      181 ttcctttccc tcccgacagc cgaaatccca acacaggttt tcggctgttt tcgccccaaa
      241 tacctcctaa ttttacccaa ataccccctt aatcctcccc ggatacccga taatcaggca
      301 tccgggctgc cttttaggcg gcagcgggca cacttagcct gttggccgct ttcaacaggt
      361 tcaaacacat cgccttcaga tggctttgcg cactcacttt aatcagtccg aaataggctg
      421 cccgcgcata gcggaattta cggtgcagcg taccgaagct ttgttcgacc acataacggg
      481 tcttcgataa ataccggttg cgtttggttt gcgtttccg
//
LOCUS       DAWHRA010000274          510 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWHRA010000274 DAWHRA010000000
VERSION     DAWHRA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.274"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          141 a           88 c          125 g          156 t
ORIGIN      
        1 ttttaaaatt tttatattca catagatggg gatgatggga tttaggattc tgattttgtt
       61 tttgagagaa tgaaggaatt tgagattgtg ggcgattatc gggaaaaata gaatctttcc
      121 gccgtcattc ccgcgcaggc gggaatctag acattcaatg ctaaggcaat ttatcgggaa
      181 tgactgaaac tcaaaaaact agattcccac tttcgtggga atgacgaatt tcaggttgct
      241 gtttttggtt ttctgttttt gtgaaaataa caggatttta gcttgtgggt atttaccgga
      301 aaaaacagaa accgctccgc cgtcattccc gcgaaggcgg gaatctaggt ctgtcggtgc
      361 ggaaacttat cggataaaac ggtttcttca gattttacgt cctagattcc cactttcgtg
      421 ggaatgacgg ggttaaagtt tcaaaattta ttctaaatag ctgaaactca acgcactgga
      481 ttcccgcctg cgcgggaatg acgaagtgga
//
LOCUS       DAWHRA010000275          508 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWHRA010000275 DAWHRA010000000
VERSION     DAWHRA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.275"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(340..498)
                     /locus_tag="WIB30_002135"
     CDS             complement(340..498)
                     /locus_tag="WIB30_002135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798125.1"
                     /translation="MAENLPFPSFIHLLFLEFAFTGRGGAPRTPGFGKPPDSAPAELL
                     VEKLTSPF"
BASE COUNT          127 a          121 c          147 g          113 t
ORIGIN      
        1 cgcggtgcag gtttccgtgc ggatggattc gtcattcccg cgtaggcggg aatctagacc
       61 ttgggataac agcaatattc aaaggttata aaagacccgt cattcccgcg caggcgggaa
      121 tctagacctt agaacaacag taatattcaa agattatctg aaagtccgag attctagatt
      181 cccactttcg tgggaatgac gggatgtagg ttcgcgggaa tgacgcggtg caggtttccg
      241 tgcggatgga ttcgtcattc ccgcgcaggc gggaatccag actgtcgggc atctgcagcg
      301 gtttgctaaa aaacgcttta ccgtgatcag tgtgcaaagt taaaatgggg aggtaagctt
      361 ttcaaccagc aattcggcgg gcgcggaatc gggcggttta ccgaaccccg gcgttcgcgg
      421 cgcgccgccc cgtcccgtga aggcaaactc aaggaataaa agatgaataa aacttggaaa
      481 cggcaggttt tccgccatac cgcgcttt
//
LOCUS       DAWHRA010000276          498 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWHRA010000276 DAWHRA010000000
VERSION     DAWHRA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.276"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1..133)
                     /locus_tag="WIB30_002136"
                     /pseudo
     CDS             complement(1..133)
                     /locus_tag="WIB30_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(217..>498)
                     /locus_tag="WIB30_002137"
     CDS             complement(217..>498)
                     /locus_tag="WIB30_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798126.1"
                     /translation="IYSRQGESLIGIGIREAALPVPTAIDILNLFINERILVLGGDIY
                     IKKDNYFYQTYDNWYYEGSNLFNSINKAMHYLSQIKLENAYVSFVLKFI"
BASE COUNT          146 a           91 c           79 g          182 t
ORIGIN      
        1 tggcagagtt gataccgtct tttaccgcaa aagcactgtc ttgtaatgcg gttgcagccg
       61 caagttgtgc ttcgggactt aaatttccta aatgattaat accttgtaga gtctgctgag
      121 cggctgtatc tgagccggtc agtgtaggtt ggattaaaac ccagcatttt caacatcttg
      181 atgttttact ataaatctat tcttgtgctt cctttgttag ataaatttca acacaaaaga
      241 tacgtatgca ttctctaatt ttatttgaga taaataatgc attgctttgt tgatactgtt
      301 aaataaatta cttccctcgt aataccaatt atcatatgtt tgataaaaat aattatcttt
      361 cttgatataa atatctcccc ccaatacaag tattctttca tttataaata aatttaatat
      421 atctattgca gtggggacgg gtaatgcggc ttctctaatt ccaattccta tcaaagattc
      481 cccttgtctc gaataaat
//
LOCUS       DAWHRA010000277          491 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWHRA010000277 DAWHRA010000000
VERSION     DAWHRA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.277"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..456
                     /locus_tag="WIB30_002138"
     CDS             <1..456
                     /locus_tag="WIB30_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HEZ3798127.1"
                     /translation="LKDFTAARRREGKKLGEHLLQRLESMEEIIDALSEFFPTLLETH
                     KEKIRARLVEAVGDINNDRLQQEFALFIQKSDIDEEFSRLRTHIAEVRRIITEHKGSS
                     GKRLDFLMQELNREANTLGSKSIAAECTQASVELKVLIEQMREQVQNIE"
BASE COUNT          153 a          134 c          114 g           90 t
ORIGIN      
        1 ttaaaagact ttactgccgc acgccggcgc gaagggaaaa aattgggcga gcacctgtta
       61 caacgcctcg aaagcatgga agaaatcata gatgcattga gcgaattctt tcctaccctg
      121 ctggaaacac ataaagaaaa aatccgcgcc cgcctcgtcg aagcagtcgg cgacatcaat
      181 aatgacaggc tgcaacagga atttgccctc tttattcaaa aatccgacat cgacgaagaa
      241 ttcagccgcc tacgcaccca cattgccgaa gtgcgtcgta tcattaccga acacaaaggc
      301 agcagcggca aacggctgga cttcctgatg caggaattaa accgcgaagc caacactttg
      361 ggcagcaaat ccattgccgc cgaatgcacc caagcctcgg tcgagctgaa agtcctgatc
      421 gaacagatgc gcgaacaggt gcagaatatc gaataatcgg tttccccgat aagcaaatgc
      481 cgtctgaaag c
//
LOCUS       DAWHRA010000278          484 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWHRA010000278 DAWHRA010000000
VERSION     DAWHRA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.278"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          146 a          121 c          118 g           99 t
ORIGIN      
        1 caaaaattcc gatgccgtct gaaatttcgt cattcccgtg aaagcgggaa tccaaccccg
       61 tcggagcaga aacttacacc ccgtcattcc cgcaaaagcg ggaatccagt aaccgaaaaa
      121 ccacgggaat ttatcggaaa aacagaaacc cccgaccgtc attcccgcga acgcgggaat
      181 ccagtaaccg aaaaaccacg ggaatctatc ggaaaaaaca gaaaccctcg accgtcattc
      241 ccgcgaacgc gggaatctag aacgtagaat ctgagaaacc gttttactcg ataaatttcc
      301 atgccgaggg gtctggattc ccgcgttcgc gggaatgacg gcatattttt tgcatttgat
      361 ataaagggtc gtttgaattt tgttcagcaa gtgcaaagtg ttgcacataa aagggcgcag
      421 gatagaggca aagcgggcgt aggtcgggct gtagcaactg tatttttcac cccgtcgggc
      481 aaaa
//
LOCUS       DAWHRA010000279          484 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWHRA010000279 DAWHRA010000000
VERSION     DAWHRA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.279"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..377
                     /locus_tag="WIB30_002139"
     CDS             <1..377
                     /locus_tag="WIB30_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3798128.1"
                     /translation="ANAHECKHLPPLLEGLPKGTTVYADKGYDSAENRQHLEERQLLD
                     GIMRKACRNRPLAEAQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQS
                     HLKAMCLNLLKAANRLSAPAAA"
BASE COUNT          124 a          121 c          142 g           97 t
ORIGIN      
        1 ccgccaatgc ccatgagtgc aaacacctgc cgcctttgtt ggaaggactg cccaaaggta
       61 cgaccgtcta tgccgacaaa ggctatgaca gtgcggaaaa ccggcaacat ctggaagagc
      121 gtcagttgtt agacggtatt atgcgcaaag cctgccgcaa ccgtccgctg gcggaagcgc
      181 aaaccaaacg taaccgatat ttgtcgaaga cccgttatgt ggtcgaacaa agcttcggta
      241 cgctgcaccg taaattccgc tatgcccggg cggcctattt cggactgatt aaagtgagtg
      301 cacaaagcca cctgaaggcg atgtgtttga acctgttgaa agccgccaac aggctaagtg
      361 cgcccgctgc cgcctaaaag gcagcacgga tgcctgatta tcgggtatcc ggggaggatt
      421 aagggggcgt ttgggtagaa ttaggcggta tttggggcga aaacagccga aaacctgtgt
      481 ttgg
//
LOCUS       DAWHRA010000280          470 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWHRA010000280 DAWHRA010000000
VERSION     DAWHRA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.280"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>470
                     /locus_tag="WIB30_002140"
     CDS             <1..>470
                     /locus_tag="WIB30_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3798129.1"
                     /translation="VVDATIIQTAGSKQRQAIEVDEEGQISSQTTPSKDSDARWIKKN
                     GLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLSPLLEGIAEGTTVYADKGYDSAE
                     NRQHLKEHQLLDGIMRKAHRNRPLTEAQTKRNRYLSKTRYVVEQSFGTLHRKFR"
BASE COUNT          147 a          130 c          113 g           80 t
ORIGIN      
        1 gtcgttgatg ccaccattat tcagaccgcc ggcagcaaac agcgtcaggc catagaagtt
       61 gacgaagaag gacaaatcag cagccaaacc acaccgagta aggacagcga tgcccgttgg
      121 ataaagaaaa acggcctcta caaactcggt tacaaacaac atacccgtac cgatgcggaa
      181 ggctatatcg agaaactgca cattactccc gccaatgccc atgagtgcaa acacctgtcg
      241 cctttgctgg aagggatagc cgaaggtacg accgtctatg ccgacaaagg ctatgacagt
      301 gcggaaaacc ggcaacatct gaaagaacat cagttgttgg acggcattat gcgcaaagcc
      361 caccgcaacc gtccgctgac ggaagcgcaa accaaacgca accgatattt gtcgaagacc
      421 cgttatgtgg tcgaacaaag cttcggtacg ctgcaccgta aattccgcta
//
LOCUS       DAWHRA010000281          464 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.281, whole genome shotgun sequence.
ACCESSION   DAWHRA010000281 DAWHRA010000000
VERSION     DAWHRA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.281"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>464
                     /locus_tag="WIB30_002141"
     CDS             <1..>464
                     /locus_tag="WIB30_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ3798130.1"
                     /translation="SGSKGWVVKLKEGQRVTVKPTVVLRTAFVTIHKYTGTDKCGAET
                     AILGINTADGGKLTKKSARPIVPDTNTKVAQYSGDKQTANGKSIPIGCMEKDGGTVCP
                     NGYVYDKPVNVRYLDEKKTDDFPVTADGDAGGSGTFKEGKKPARNNRCFSGKG"
BASE COUNT          136 a          117 c          133 g           78 t
ORIGIN      
        1 tcgggcagta aggggtgggt agtgaagctg aaggaaggac agcgcgttac cgtcaaaccg
       61 accgtggtat tgcgtaccgc ctttgtaacc atccataaat atacgggtac ggacaaatgc
      121 ggcgcggaaa ccgccatttt gggcatcaat accgccgacg gcggcaagct gaccaagaaa
      181 agcgcgcgcc cgattgtgcc ggacaccaat acgaaggtcg cgcaatattc cggcgataag
      241 caaaccgcca acggcaaatc catccctata ggttgtatgg aaaaagacgg gggaaccgtc
      301 tgcccgaacg gatatgttta cgacaaaccg gttaatgtgc gttatctgga tgaaaagaaa
      361 acagacgatt tccccgtcac ggcagacggt gatgcaggcg gcagcggaac attcaaagag
      421 ggtaaaaaac ccgcccgcaa taaccggtgc ttctccggaa aagg
//
LOCUS       DAWHRA010000282          460 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.282, whole genome shotgun sequence.
ACCESSION   DAWHRA010000282 DAWHRA010000000
VERSION     DAWHRA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.282"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          150 a          137 c           99 g           74 t
ORIGIN      
        1 aataaagtgt aatccctttg atcataaggg aatgttgaca attagatcga ttctcctagg
       61 ctgactttcg caataaatgg ggttgtcaat aattgtgtat ggtcgtttgc cgtccggcgg
      121 ccgtcattcc cgcgcaggcg ggaatccagc aacacaacgc agcaggactt tatcggaaaa
      181 aaccgaaacc ccaccgaccg tcattcccgc aaaagcggga atccaaaaac gcaacgcaac
      241 aggaatttat cggaaaaaac cgaaacccca ccgccgtcat tcccgcaaaa gcgggaatcc
      301 agcaaccgaa aaaccacagg aatctatcag caaaaacaga aacccccacc gccgtcattc
      361 ccgcgcaggc gggaatccag caacacaacg cggcaggact ttatcggaaa aaacagaaac
      421 cccaccgccg tcattcccgc aaaagcggga atccagaccc
//
LOCUS       DAWHRA010000283          459 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.283, whole genome shotgun sequence.
ACCESSION   DAWHRA010000283 DAWHRA010000000
VERSION     DAWHRA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.283"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>459
                     /locus_tag="WIB30_002142"
     CDS             <1..>459
                     /locus_tag="WIB30_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3798131.1"
                     /translation="DYSTLCRYRNWLAQDDTLSELLKLINRQLTEKGLKVEKASAAVV
                     DATIIQTAGSKQRQAIEVDEEGQVSGQTTPSKDKDARWTKKNGLYRLGYKQHTRTDEE
                     GYIKKLHITPANTHECKHLLPLLEGLPKGTTVYADKGYDSAENRQHLKEHQ"
BASE COUNT          154 a          127 c          105 g           73 t
ORIGIN      
        1 gattacagca ccttatgccg ctaccgcaac tggctggcgc aagacgacac cctgtccgaa
       61 ttgctcaaac tgattaaccg acaactgacc gaaaaaggcc taaaagtaga gaaagcatcc
      121 gccgccgtcg ttgacgccac cattattcag accgccggca gcaaacagcg ccaggccata
      181 gaagtcgacg aggaaggaca agtcagcggc caaaccacac cgagtaaaga taaagatgcc
      241 cgttggacaa agaaaaacgg tttatacaga ctcggttaca aacaacatac ccgtaccgat
      301 gaggaaggct atatcaagaa actgcacatc accccagcca atacccatga gtgcaaacac
      361 ctgttgcctt tgttggaagg gttacccaaa ggtacgaccg tctatgccga caaaggctat
      421 gacagtgcgg aaaaccggca acatctgaaa gaacatcag
//
LOCUS       DAWHRA010000284          455 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.284, whole genome shotgun sequence.
ACCESSION   DAWHRA010000284 DAWHRA010000000
VERSION     DAWHRA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.284"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(305..>455)
                     /locus_tag="WIB30_002143"
     CDS             complement(305..>455)
                     /locus_tag="WIB30_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3798132.1"
                     /translation="GSVKWFYGQPVKRDNASADAVKADTAANGKQIDTKHLPSTASTR
                     KSTPN"
BASE COUNT           97 a           92 c          123 g          143 t
ORIGIN      
        1 cggaaactta tcgggaaaac ggtttcttga gattttgcgt tctggattcc cactttcgtg
       61 ggaatgacgg gatttaagtt tcaaaattta ttctaaataa ctgaaactca acgaactaga
      121 ttcccgcttt tgcgggaatg acgaatttta ggttgctgtt tttggttttc tgtccttgtg
      181 ggaatgatga aaatttaagt tttaggaatt tatcggaaaa aacagaaacc gctccgccgt
      241 cattcccgcg caggcgggaa tccagccttg tcggtacgga aacttatcgg gtaaaaagtt
      301 ttctctagtt tggtgtcgat tttcttgtcg atgctgttga cggcaggtgc ttggtgtcga
      361 tctgcttgcc gttggcggcg gtgtcggctt tgacggcgtc ggcgctggcg ttgtcgcgct
      421 taaccggctg tccgtagaac catttgaccg aaccg
//
LOCUS       DAWHRA010000285          442 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.285, whole genome shotgun sequence.
ACCESSION   DAWHRA010000285 DAWHRA010000000
VERSION     DAWHRA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.285"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            147..>442
                     /locus_tag="WIB30_002144"
     CDS             147..>442
                     /locus_tag="WIB30_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ3798133.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFVDEGGIVAVQIAGSNPQQMADAARYNVSLGAQVIDINMG"
BASE COUNT           98 a          123 c          130 g           91 t
ORIGIN      
        1 tgcccgcctg tgagtggcgg ggcagggaat ctgtgtgcgg attatgccat aaaacggtgt
       61 ccgacccaat cgcgggcgcg tccggagatt ggaaatcctg cttaaaaaat gtacaatggc
      121 gtactttttt gaaacgcggg cccattatgc acatcggcgg ctattttatc gacaacccca
      181 tcgcacttgc accgatggcg ggcattaccg acaaaccctt ccgccgcctc tgccgcgatt
      241 tcggcgcggg ttgggcggtg tgcgaaatgc tgaccagcga cccgacgctc agaaatacta
      301 gaaaaacctt gcaccgcagt gattttgtcg atgaaggcgg catcgttgcc gtgcagattg
      361 ccggcagcaa tccgcagcag atggcggatg ccgcgcgtta taacgtcagc ctcggagcgc
      421 aggtcatcga catcaacatg gg
//
LOCUS       DAWHRA010000286          436 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.286, whole genome shotgun sequence.
ACCESSION   DAWHRA010000286 DAWHRA010000000
VERSION     DAWHRA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.286"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..180
                     /locus_tag="WIB30_002145"
     CDS             <1..180
                     /locus_tag="WIB30_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3798134.1"
                     /translation="YLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCL
                     NLLKAANRLSAPICA"
BASE COUNT           97 a          104 c          127 g          108 t
ORIGIN      
        1 tatttgtcga agacccgtta tgtggtcgaa caaagcttcg gtacgctgca ccgtaaattc
       61 cgctatgcgc gggcagccta tttcgggctg attaaagtga gtgcgcaaag ccatctgaag
      121 gcgatgtgtt tgaacctttt aaaagctgcc aacaggctaa gtgcgcctat ttgcgcctaa
      181 aaggcggttc ggatgcctga ttatcgggta tccgggcagg attaaggggg tgtttgggta
      241 aaattaggcg gtatttgggg cgaaaacagc cgaaaacctg tgttgggatt tcggttgtcg
      301 tgagggaaag gaattttgca aaggtctcaa gctatgccgg ctgctgccgc ccacttcatc
      361 tgcgccaaat cctgccgccc accgccgaag ccctcatgcg ttcgcggtac agcgcgtatg
      421 tgctgcacct tatcga
//
LOCUS       DAWHRA010000287          431 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.287, whole genome shotgun sequence.
ACCESSION   DAWHRA010000287 DAWHRA010000000
VERSION     DAWHRA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.287"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            1..431
                     /locus_tag="WIB30_002146"
                     /pseudo
     CDS             1..431
                     /locus_tag="WIB30_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          147 a          112 c          103 g           69 t
ORIGIN      
        1 caactgaccg aaaaaggttt aaaagtagag aaagcatccg ccgccgtcat tgacgccacc
       61 attattcaga ccgccgacgg caaacagcgt caggccatag aagtcgatga agaaggacaa
      121 gtcagcggcc aaaccacacc gagtaaggac agcgatgcgc gttggatcaa gaaaaacggc
      181 ctctacaaac tcggttacaa acaacatacc cgcaccgatg cggaaggcta tatcgagaaa
      241 ctgcacatta tagtagatta aatttaaatc aggacaaggc gacgaagccg cagacagtac
      301 agatagtacg gcaaggcgag gcaacgccgt actggtttaa atttaatcca ctatattatg
      361 cgcaaagcct gccgcaaccg tccgctgacg gaggcgcaaa ccaaacgcaa ccgatatttg
      421 tcgaagaccc g
//
LOCUS       DAWHRA010000288          430 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.288, whole genome shotgun sequence.
ACCESSION   DAWHRA010000288 DAWHRA010000000
VERSION     DAWHRA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.288"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          117 a           86 c          120 g          107 t
ORIGIN      
        1 ttggacagcg gcaatattca aagattatct gaaagtccga gattctagat tcccactttc
       61 gtgggaatga cgggatgtag gttcgtggga atgacgggat ttgagattgc ggcatttatc
      121 ggaaaaaaca gaaaccgctc tgccgtcatt cccgcgcagg cgggaatcca gaccattgga
      181 cagcagcaat attcaaagat tatctgaaag tccgggattc tagattccca ctttcgtggg
      241 aatgacggga tgtaggttcg tgggaatgac gggatttgag attgcggcat ttatcggaaa
      301 aacagcaacc gctccgccgt tattcccgcg caggcgggaa tctagacctt gggataacag
      361 caatattcaa aggttagctg aagctttaga gattctggat tcccactttc gtgggaatga
      421 cggaatgtag
//
LOCUS       DAWHRA010000289          412 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.289, whole genome shotgun sequence.
ACCESSION   DAWHRA010000289 DAWHRA010000000
VERSION     DAWHRA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.289"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          139 a           50 c           82 g          141 t
ORIGIN      
        1 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atacgtccta gattcccact
       61 ttcgtgggaa tgacgagatg tgggtttttg tgcggatttg aaccggtaag ggtggtgtgg
      121 gattggtggt ttgcttagga tcttttggat tgtattttgt atatacattt acctcaaaaa
      181 tttccataac tgaaccttta cttgttgata aaagataaaa taaaattaga aactaaaagt
      241 gagaaaaaat taataataat agggatgtat aaatgtaaag gctccgtttc atagctaagg
      301 ttatctgaat atatggaaaa aaagtaaaag tccataaaag taaaatatat agataatgct
      361 aatgataata gaattatcac tttcctagag taaggataat attttttacg at
//
LOCUS       DAWHRA010000290          408 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.290, whole genome shotgun sequence.
ACCESSION   DAWHRA010000290 DAWHRA010000000
VERSION     DAWHRA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.290"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..282)
                     /locus_tag="WIB30_002147"
     CDS             complement(<1..282)
                     /locus_tag="WIB30_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3798135.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRL"
BASE COUNT          107 a           96 c           96 g          109 t
ORIGIN      
        1 aagcctgaga ttgccgccga ttaaatattg gctgtgccgc ttgatttgcc gccagcgttc
       61 cgaataagca gaatcattgt ccggcagccg ccatatggta tgcagatggt cgggcatcaa
      121 cacccatgcc aaaatttcaa acggataccg ttcgcgcacc gccattaccg cctgccgtaa
      181 agccaaacgc accgcatcat cggtcaaaat cttctgccgt ttattggtta caaccgtaaa
      241 aaagtaagtg ccgccattgc ggtaaaaacg acggtatttc atagtattat gctcggaatg
      301 attttgtagg tcggattctt gaattcgaca ttttgggcat tgctgcaatg gattgcaatg
      361 atgggaatgt taaaggtttt gtcggataca agtatccgac ctacgctt
//
LOCUS       DAWHRA010000291          408 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.291, whole genome shotgun sequence.
ACCESSION   DAWHRA010000291 DAWHRA010000000
VERSION     DAWHRA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.291"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            8..>408
                     /locus_tag="WIB30_002148"
     CDS             8..>408
                     /locus_tag="WIB30_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ3798136.1"
                     /translation="MSVMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARCGLLFAT
                     LKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAEYRAQNG
                     AFKSVDDLTKVKGIGPAVLAKLKDQASAGAPA"
BASE COUNT           88 a           95 c          130 g           95 t
ORIGIN      
        1 ggaaaaaatg tcggtgatgg cgggacggca tccgtacggc gtccggtcgg gtttgcggag
       61 gaacggcttg aaactttggg atattcattt tagaatgact cgttttatcg tcgcaagatg
      121 cggtttattg tttgcaaccc ttaaaggaaa aaccatgaag aaaatgttcg tgctgttctg
      181 tatgctgttc tcctgcgcct tctcccttgc ggcggtaaac atcaatgcgg cttcgcagca
      241 ggagttggag gcgctgccgg gcataggccc ggcgaaggcg aaggccattg cggaataccg
      301 tgcgcaaaac ggtgcgttca agtctgtaga cgatttgacc aaggtaaagg gcatcggccc
      361 tgcggtgctg gcgaagctga aggatcaggc ttctgccggc gcgcccgc
//
LOCUS       DAWHRA010000292          406 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.292, whole genome shotgun sequence.
ACCESSION   DAWHRA010000292 DAWHRA010000000
VERSION     DAWHRA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.292"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          103 a          109 c           99 g           95 t
ORIGIN      
        1 cggcactagc caaaaaaacc gaaaccgaca ggtctagatt cccgcctgcg ggggaatgac
       61 ggattttagg ttggggtcat ttattgggaa aagcagaaac cgctccgccg tcattcccac
      121 gaaagtggga atttaggatg cagggttggg gaaaccgtgt tatccgataa gtttccgcac
      181 cgacaggtct agattcccgc ctgcgcggga atgacgcatc catccgtacg gaaacctgca
      241 ccacgtcatt cccacgaaag tgggaatcta gttttttgag tttcagtcat tcccgataaa
      301 ttgccttagc attgaatgtc tagattcccg cctgtgcggg aatgacgcat ccatccgtac
      361 ggaaacctgc accacgtcat tcccacgaac ctacattccg ttattc
//
LOCUS       DAWHRA010000293          394 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.293, whole genome shotgun sequence.
ACCESSION   DAWHRA010000293 DAWHRA010000000
VERSION     DAWHRA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.293"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          127 a           65 c           75 g          127 t
ORIGIN      
        1 ggtttaaaag ttacccgaaa cctcaaaaaa aaaaggctgt gttttaatat agtgttgata
       61 gacgtacttg gcttccatgt attcaatcgt ggaaatctat atcttcgtcc tcgccgaaat
      121 agtctatgcc cgatatacaa ttttgataca caaacttgga aatatgggta tcgtcgccgg
      181 agcgatagaa tgcggacagt tatatatata tggttttttt agggaagcgg gcagatgaag
      241 ggcggatttt gaattaaccc catgcaaatt gacttttgcg ggcggtttta cctcccatat
      301 atttacaaaa gccaaatttt taaacatata tccttgatat atacacggcg taaatatata
      361 ctggaaacat ctttaaattt tttgaaattt taaa
//
LOCUS       DAWHRA010000294          385 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.294, whole genome shotgun sequence.
ACCESSION   DAWHRA010000294 DAWHRA010000000
VERSION     DAWHRA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.294"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          116 a           96 c           75 g           98 t
ORIGIN      
        1 accgattcac ttggtgcttc agcaccttag acaatcgttc tctttgagct aaggcgaggc
       61 aacgccgtac tggtttttgt taatccacta tacatcccgt cattcccacg aaagtgggaa
      121 tctagaaatt taatgttgcg gcactagcca aaaaaaccga aaccgaacgg actagattcc
      181 cgcctgcgcg ggaatgacgg cggagcggtt tctgtttttt ccggtaaata cccacaagct
      241 aaaatcccat tattttcaca aaaacagaaa accaaaaaca gcaacctgaa attcgtcatt
      301 cccgctcagg cgggaatcca gtgcgttgag tttcagctat ttagaataaa ttttgaaact
      361 ctaatcgcgt cattcccaca aaagt
//
LOCUS       DAWHRA010000295          375 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.295, whole genome shotgun sequence.
ACCESSION   DAWHRA010000295 DAWHRA010000000
VERSION     DAWHRA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.295"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(74..>375)
                     /locus_tag="WIB30_002149"
     CDS             complement(74..>375)
                     /locus_tag="WIB30_002149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3798137.1"
                     /translation="GAGASPRKAFQLRKPRPKGRRSTAGLSGSQATGRRSTAELGGSQ
                     ATGRRSTAGWAEATAERQGEAPPGLGGSHGRKAGEVPPVWAEAMVKGRRSTAGLQ"
BASE COUNT           51 a          138 c          102 g           84 t
ORIGIN      
        1 aaagaatcat aaaggcgaaa aaaccccgcc tttacgattc tttacggaca tcctccccct
       61 gcctgaacaa aggttactga agcccggcgg tgcttcgcct gccttttacc atggcttccg
      121 cccagaccgg cggtacttcg cctgcctttc ggccgtggct tccgcctaag cctggcggtg
      181 cttcgccctg cctttcggcc gtggcttccg cccaaccggc ggtgcttcgc ctgcctgtag
      241 cctggcttcc gcctagctcg gcggtgctgc gcctgcctgt agcctggctt ccgctcagac
      301 cggcagtgct gcgcctgcct ttcggccgtg gcttccgaag ctgaaaggct ttgcggggac
      361 tagcccccgc acccc
//
LOCUS       DAWHRA010000296          374 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.296, whole genome shotgun sequence.
ACCESSION   DAWHRA010000296 DAWHRA010000000
VERSION     DAWHRA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.296"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           70 a           85 c          116 g          103 t
ORIGIN      
        1 gggtactcat catcaacctg ccttgcattc ggttgtgttg tccggcaact gtccggttgt
       61 gtcgatgggt tgccctgccg ctccctgtgc tgcgcaacgg ttgtgtgcct tgggtgggcg
      121 cgggtggcgg ccgggcggtt tgttgctatg atacgggagt tccgatatac aagggtgggc
      181 ttcagcccac caatcaacga ccgaaaaaga aaaaacgttg ggctgaagcc cactctacgc
      241 aattacgcaa ctgttatcgt ggggatgacg ggattttagg tttttgtttt tctgtttttg
      301 cgggaaggat gggttttagg ttgcggtcat ttatcggaaa agcagaaatc gctccgccgt
      361 cattcccgcg aaag
//
LOCUS       DAWHRA010000297          345 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.297, whole genome shotgun sequence.
ACCESSION   DAWHRA010000297 DAWHRA010000000
VERSION     DAWHRA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.297"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          100 a           76 c           73 g           96 t
ORIGIN      
        1 atccactata gtttttttga atttcgggca acgcttgaat cttcattccg cgcaggcgga
       61 aattatcggt gcggtacggc aacttttttc gatatgaaaa gaccgtcatt cctgtaaaaa
      121 caaaaaatca aaaacagaaa attgaaattc gtcattcccg cgcaggcggg aatccaggac
      181 gtaaaatcta tagtggatta acaaaaacca gtacagcgtt gccttgactt agctcgaaga
      241 gaacgattct ctaaggtgct gaagcaccga gtgagtcggt tccgtactat ccgtactgtc
      301 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataaa
//
LOCUS       DAWHRA010000298          342 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.298, whole genome shotgun sequence.
ACCESSION   DAWHRA010000298 DAWHRA010000000
VERSION     DAWHRA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.298"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           88 a          103 c           82 g           69 t
ORIGIN      
        1 cacacaggcg ggaatccaga cccctgacgc ggcgggaatc tatcggaaat gactgaaacc
       61 ccgagattct agattcccac tttcgtggga atgacggttc agttgcgttc cgacaacacc
      121 gcaatctcga aatccgtcat tcccgcacag gcgggaatcc agacccctga cgcggcggga
      181 atctatcgga aatgactgaa accccgagat tctagattcc cactttcgtg ggaatggcgg
      241 ttcagttgca ttccgacaac accgtaatct tgaaatccgt cattcccgat aacagcgcaa
      301 tcttgaaacc cgtcattccc gcgcaggcgg gaatccagac ct
//
LOCUS       DAWHRA010000299          322 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.299, whole genome shotgun sequence.
ACCESSION   DAWHRA010000299 DAWHRA010000000
VERSION     DAWHRA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.299"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1..98)
                     /locus_tag="WIB30_002150"
                     /pseudo
     CDS             complement(1..98)
                     /locus_tag="WIB30_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(135..>322)
                     /locus_tag="WIB30_002151"
     CDS             complement(135..>322)
                     /locus_tag="WIB30_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3798138.1"
                     /translation="TIRDEADFACHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGI
                     YPHNWGGGNADFSIGYD"
BASE COUNT           87 a           78 c           70 g           87 t
ORIGIN      
        1 ttcgataaat atcggttacg tttggtttgc gtttccgtca gcggacggtt gcggcaggct
       61 ttgcgcataa tgccgtcctg cagccgatgc tctttcagtt ttccgtaggt cggattctcg
      121 aatccgacat tacttcaatc atatccaata gaaaagtccg cattgccgcc accccaatta
      181 tgcggataaa taccctgttt aacataacgg tgaaacgtag aaaaccccca atcggaaatt
      241 tgtcctacat agccatgttt gaccggattg aaatgcagat aatcaaaatg gcaggcaaaa
      301 tcggcctcat cgcggatagt at
//
LOCUS       DAWHRA010000300          317 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.300, whole genome shotgun sequence.
ACCESSION   DAWHRA010000300 DAWHRA010000000
VERSION     DAWHRA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.300"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           92 a           78 c           61 g           86 t
ORIGIN      
        1 aaaaaacagc aacctaaaat tcgtcattcc cgcgcaggcg ggaatccagt ccgttcggtt
       61 tcgcttgttt tgggtttcgg gtaacttcca cttcgtcatt cccgcgaaag tgggaatccg
      121 gttcgttgag tttcagtcat ttccgataaa tcaccgcaac attaaatttc tagattcccg
      181 cttttgcggg aatgacggcg gcgggatttc tgtttttccg acaaattcct aaaacttaaa
      241 atttcgttat ttccgcgaga acagaaaacc aaaaacagaa acctaaaatc tcgtcattcc
      301 cgcaaaaaca gaaaacc
//
LOCUS       DAWHRA010000301          312 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.301, whole genome shotgun sequence.
ACCESSION   DAWHRA010000301 DAWHRA010000000
VERSION     DAWHRA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.301"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           93 a           73 c           63 g           83 t
ORIGIN      
        1 cccactttcg tgggaatgac gcggaaaagt tgctgtgatt ccggataaat tttcgcaacg
       61 tttaatttcc gttttacccg ataaatgccc gcaatctcaa atcccgtcat tccccaaaaa
      121 caaaaaaatc aaaaacagaa atcccatcat tcccgcgcag gcgggaatcc aggtctgtcg
      181 gtgcggaaac ttatcggata aaacggtttc tttagatttt acgttctaga ttcccgcttt
      241 cgcgggaatg acggaatatt tttgaatttg ataaaaatgc cgtctgaaac ggtcaaacaa
      301 cgcttcagac gg
//
LOCUS       DAWHRA010000302          311 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.302, whole genome shotgun sequence.
ACCESSION   DAWHRA010000302 DAWHRA010000000
VERSION     DAWHRA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.302"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            67..>311
                     /locus_tag="WIB30_002152"
     CDS             67..>311
                     /locus_tag="WIB30_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3798139.1"
                     /translation="MSTFFQQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSL"
BASE COUNT           86 a          101 c           57 g           67 t
ORIGIN      
        1 gcggaaactt gttgaaaact ttgcaaaatc ccctaaattc ccaccaagac atttaggaga
       61 ttttccatga gcaccttctt ccagcaaacc gcacaagcca tgattgccaa acacatcaac
      121 cgcttcccgc tattgaagtt ggatcaagtg attgattggc agccgatcga acaatacctg
      181 aaccgtcaaa aaacccgtta ccttcgagac caccgcggcc gtcccgccta tccgctgctg
      241 tccatgttca aagccgtcct gctcggacaa tggcacagcc tctccgatcc cgaactcgaa
      301 cacagcctca t
//
LOCUS       DAWHRA010000303          298 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.303, whole genome shotgun sequence.
ACCESSION   DAWHRA010000303 DAWHRA010000000
VERSION     DAWHRA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.303"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(111..>298)
                     /locus_tag="WIB30_002153"
     CDS             complement(111..>298)
                     /locus_tag="WIB30_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3798140.1"
                     /translation="TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQDI
                     YPHNWGGGNADFSIGYD"
BASE COUNT           80 a           76 c           65 g           77 t
ORIGIN      
        1 gtttgcgcct ccgtcagcgg acggttgcgg caggctttgc gcataatgcc gttctgcaac
       61 cgatgctctt tcagttttcc gtaggtcgga ttctcgaatc cgacattact tcaatcgtat
      121 ccaatagaaa agtccgcatt gccgccaccc caattatgcg gataaatatc ctgtttgaca
      181 taacggtgaa acgtagaaaa cccccaatcg gaaatttgtc ctacatagcc atgtttgacc
      241 ggattgaaat gcagataatc aaaatgccag gcaaaatcgg cctcatcgcg gatagtat
//
LOCUS       DAWHRA010000304          295 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.304, whole genome shotgun sequence.
ACCESSION   DAWHRA010000304 DAWHRA010000000
VERSION     DAWHRA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.304"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>295
                     /locus_tag="WIB30_002154"
     CDS             <1..>295
                     /locus_tag="WIB30_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEZ3798141.1"
                     /translation="IYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVG
                     IENRPAIVDQKSRIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAED"
BASE COUNT           92 a           89 c           66 g           48 t
ORIGIN      
        1 catttaccgc taccttcgcc aagacaaaag caacggcagc acgttgtggc aacatctcag
       61 aatatgcagc aaaccctacc gcaaacgcta cggcagcaca tggaccagag gcaaagtacc
      121 caaccgtgtc ggcatagaaa accgacccgc tatcgtcgac cagaaatccc gtatcggcga
      181 ttgggaagcc gacaccattg tcggcaaagg acagaaaagc gcattattga ccttggtcga
      241 acgcgttacc cgctacacca tcatctgcaa attggatagc ctcaaagccg aagac
//
LOCUS       DAWHRA010000305          292 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.305, whole genome shotgun sequence.
ACCESSION   DAWHRA010000305 DAWHRA010000000
VERSION     DAWHRA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.305"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           83 a           54 c           66 g           89 t
ORIGIN      
        1 ggcgggaatc taggtttgtc ggcacggaaa cttatcgggt aaaacggttt ctttagattt
       61 tacgttctag attcccgcct gcgcgggaat gacgatgaaa agattgttgt cgcttcggat
      121 aaatttttgt cgcgttgggt tctagattcc cgcctgcgcg ggaatgacgg cggcgggttt
      181 ctgtttttcc gataaataca cacaaactaa aatttcgtca ttcccataaa aaacagaaaa
      241 ccaagtgaga ataacaattc gttgtaaaca aataactatt tgttaatttt ta
//
LOCUS       DAWHRA010000306          292 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.306, whole genome shotgun sequence.
ACCESSION   DAWHRA010000306 DAWHRA010000000
VERSION     DAWHRA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.306"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           68 a           68 c           77 g           79 t
ORIGIN      
        1 tgggtttcca tcatttccga taaactcctg ccgcgttgta tttccagatt cccgctttcg
       61 cgggaatgac ggcggatggg tttccgtacc gtggtgtccg gattcccgac ggtacgggaa
      121 tgacggcagt gcttccttgg cttagtttta ttttaatcga ctgtatggtg gattaacaaa
      181 aatcaggaca aggcaacgaa gccgcagaca gtacaaatag tacggaaccg attcacttgg
      241 tgcttcagca tcttagagaa tcgttctctt tgagctaagg cgaggcaacg cc
//
LOCUS       DAWHRA010000307          287 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.307, whole genome shotgun sequence.
ACCESSION   DAWHRA010000307 DAWHRA010000000
VERSION     DAWHRA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.307"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           73 a           73 c           58 g           83 t
ORIGIN      
        1 aacagcaact taaaacctcg tcattcccgc aaaagcggga atccagtccg ttcggtttcg
       61 gtcgtttccg ataaattccc gttgcttttc atttctagat tcccactttc gtgggaatga
      121 cggcggagag gtttttgttg tttcggagaa gtttctgcaa ccctagaatc tcgttatttc
      181 cacaaaaaac agaaaaccaa aacagcaact taaaacctcg tcattcccgc aaaagcggga
      241 atccggtccg ttcggtttcg gtcgtttccg ataaattcct gctgctt
//
LOCUS       DAWHRA010000308          280 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.308, whole genome shotgun sequence.
ACCESSION   DAWHRA010000308 DAWHRA010000000
VERSION     DAWHRA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.308"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           94 a           45 c           47 g           94 t
ORIGIN      
        1 tttaaaattt cggaaaattt aaagatgttt ccagtatata tttacgccgt gtatatatca
       61 aggatatatg tttaaaaatt tggcttttgt aaatatatgg gcggtaaaac cgcattattt
      121 tatgttgtta cctattttac accttgtctg cacacgattt ccaaacaacc taaaaagaaa
      181 cctgccgaaa ggcaggttta aagaggtcat tttaaatgtg aactaaagtt tacaccctcg
      241 gagggtatcg cgtatatcag acgtacttaa tatacactat
//
LOCUS       DAWHRA010000309          278 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.309, whole genome shotgun sequence.
ACCESSION   DAWHRA010000309 DAWHRA010000000
VERSION     DAWHRA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.309"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          114 a           58 c           43 g           63 t
ORIGIN      
        1 cctctaaaaa ataatcgctt gttttgacac cgatacactc atatagtgga ttaacaaaaa
       61 tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgcc
      121 gtactggttt aaatttaatc cactatacaa atacagaaac tcaagaaaat aaccttgtgt
      181 attgaccatc tcaagcaatt cagaaaaatc aagaaatttt ctgaccgtaa acaaacgttt
      241 ccctaaaaaa acgatgtctt caaaaatatc gaacaaat
//
LOCUS       DAWHRA010000310          268 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.310, whole genome shotgun sequence.
ACCESSION   DAWHRA010000310 DAWHRA010000000
VERSION     DAWHRA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.310"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..268
                     /locus_tag="WIB30_002155"
     CDS             <1..268
                     /locus_tag="WIB30_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3798142.1"
                     /translation="VITAEMKSSGVNKEIQGKKLSLWAKRQDGSVKWFCGLPVTRTDA
                     KADTVAAAAKTADNINTKHLPSTCRDASSVVCIETPPTAFYKNT"
BASE COUNT           79 a           88 c           57 g           44 t
ORIGIN      
        1 cgtcattacc gccgaaatga aatcaagcgg cgtaaacaaa gaaatccaag gcaaaaaact
       61 ctccctgtgg gccaagcgtc aagacggttc ggtaaaatgg ttctgcggac tgccggttac
      121 gcgcaccgac gccaaagccg acaccgtcgc cgccgccgcc aagaccgccg acaacatcaa
      181 caccaagcac ctgccgtcaa cctgccgcga cgcttcatct gtcgtttgca tagaaacacc
      241 acctacggct ttctataaaa atacctaa
//
LOCUS       DAWHRA010000311          264 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02972
            SAMN17286664-rid13897423.denovo.311, whole genome shotgun sequence.
ACCESSION   DAWHRA010000311 DAWHRA010000000
VERSION     DAWHRA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286664
            Sequence Read Archive: SRR13407380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243159.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:24:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 1,986
            CDSs (with protein)               :: 1,986
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 53 of 113
            Pseudo Genes (incomplete)         :: 64 of 113
            Pseudo Genes (internal stop)      :: 13 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286664-rid13897423.denovo.311"
                     /strain="NM02972"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2007"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           68 a           50 c           68 g           78 t
ORIGIN      
        1 agattcccgc ctgcgcggga atgacgaatt tcaggtttct gtttttggtt ttctgttttt
       61 gtgaaaataa caggatttta gcttgtgggt atttaccgga aaaaatagaa accgctccgc
      121 cgtcattccc gcgcaggcgg gaatccagac attcaatgct gaggcaattt atcggaaatg
      181 actgaaactc aaaaaactgg attcccactt tcgtgggaat gacgggatgt aggttcgtgg
      241 gaatgacgaa tttcaggttt ctgt
//
